LOCUS       DAKTIP010000001        51390 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAKTIP010000001 DAKTIP010000000
VERSION     DAKTIP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 51390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51390
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.001"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            40..1038
                     /locus_tag="O0305_000001"
     CDS             40..1038
                     /locus_tag="O0305_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0273188.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1270..2151
                     /gene="rbsU"
                     /locus_tag="O0305_000002"
     CDS             1270..2151
                     /gene="rbsU"
                     /locus_tag="O0305_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCY0273189.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2166..2570
                     /gene="rbsD"
                     /locus_tag="O0305_000003"
     CDS             2166..2570
                     /gene="rbsD"
                     /locus_tag="O0305_000003"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCY0273190.1"
                     /translation="MKKSAILNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2598..3512
                     /gene="rbsK"
                     /locus_tag="O0305_000004"
     CDS             2598..3512
                     /gene="rbsK"
                     /locus_tag="O0305_000004"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCY0273191.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKVAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSIGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3610..3744)
                     /locus_tag="O0305_000005"
     CDS             complement(3610..3744)
                     /locus_tag="O0305_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273192.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3763..4524
                     /locus_tag="O0305_000006"
     CDS             3763..4524
                     /locus_tag="O0305_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0273193.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(4958..6394)
                     /locus_tag="O0305_000007"
     CDS             complement(4958..6394)
                     /locus_tag="O0305_000007"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCY0273194.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6410..7201)
                     /locus_tag="O0305_000008"
     CDS             complement(6410..7201)
                     /locus_tag="O0305_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0273195.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDTQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAVGIQSEDGVDIVIHIGLNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7350..8054
                     /locus_tag="O0305_000009"
     CDS             7350..8054
                     /locus_tag="O0305_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0273196.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8162..8863)
                     /gene="lrgB"
                     /locus_tag="O0305_000010"
     CDS             complement(8162..8863)
                     /gene="lrgB"
                     /locus_tag="O0305_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCY0273197.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(8856..9299)
                     /gene="lrgA"
                     /locus_tag="O0305_000011"
     CDS             complement(8856..9299)
                     /gene="lrgA"
                     /locus_tag="O0305_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCY0273198.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9412..10152)
                     /locus_tag="O0305_000012"
     CDS             complement(9412..10152)
                     /locus_tag="O0305_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCY0273199.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNNASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10155..11909)
                     /locus_tag="O0305_000013"
     CDS             complement(10155..11909)
                     /locus_tag="O0305_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0273200.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(12156..12830)
                     /gene="scdA"
                     /locus_tag="O0305_000014"
     CDS             complement(12156..12830)
                     /gene="scdA"
                     /locus_tag="O0305_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCY0273201.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAALEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQSAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGELLVKMSELTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(12970..14694)
                     /gene="tarS"
                     /locus_tag="O0305_000015"
     CDS             complement(12970..14694)
                     /gene="tarS"
                     /locus_tag="O0305_000015"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0273202.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKNQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFKGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVQILLSKDIKKRDFKNIVKLNTA
                     HITYPLNK"
     gene            complement(14727..16415)
                     /locus_tag="O0305_000016"
     CDS             complement(14727..16415)
                     /locus_tag="O0305_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0273203.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(16437..17462)
                     /locus_tag="O0305_000017"
     CDS             complement(16437..17462)
                     /locus_tag="O0305_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0273204.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(17455..18171)
                     /locus_tag="O0305_000018"
     CDS             complement(17455..18171)
                     /locus_tag="O0305_000018"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCY0273205.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(18447..19616)
                     /locus_tag="O0305_000019"
     CDS             complement(18447..19616)
                     /locus_tag="O0305_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol--glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HCY0273206.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQNAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGQASQRICQTIFK"
     gene            complement(20044..21738)
                     /locus_tag="O0305_000020"
     CDS             complement(20044..21738)
                     /locus_tag="O0305_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0273207.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     FESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(21760..22785)
                     /locus_tag="O0305_000021"
     CDS             complement(21760..22785)
                     /locus_tag="O0305_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCY0273208.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(22778..23494)
                     /locus_tag="O0305_000022"
     CDS             complement(22778..23494)
                     /locus_tag="O0305_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCY0273209.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(24022..25065)
                     /locus_tag="O0305_000023"
     CDS             complement(24022..25065)
                     /locus_tag="O0305_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0273210.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(25089..25235)
                     /locus_tag="O0305_000024"
     CDS             complement(25089..25235)
                     /locus_tag="O0305_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273211.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(25237..26292)
                     /locus_tag="O0305_000025"
     CDS             complement(25237..26292)
                     /locus_tag="O0305_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCY0273212.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAYHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKVLLKPLDLDENEGEN"
     gene            complement(26310..27569)
                     /locus_tag="O0305_000026"
     CDS             complement(26310..27569)
                     /locus_tag="O0305_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCY0273213.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(27796..28074)
                     /locus_tag="O0305_000027"
     CDS             complement(27796..28074)
                     /locus_tag="O0305_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0273214.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(28097..28564)
                     /locus_tag="O0305_000028"
     CDS             complement(28097..28564)
                     /locus_tag="O0305_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0273215.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(28549..30645)
                     /locus_tag="O0305_000029"
     CDS             complement(28549..30645)
                     /locus_tag="O0305_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0273216.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            complement(30776..30874)
                     /locus_tag="O0305_000030"
     CDS             complement(30776..30874)
                     /locus_tag="O0305_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273217.1"
                     /translation="MYVKNASKKHKTQKYMQKFQLLYFKKLKSQKI"
     gene            complement(30984..31919)
                     /locus_tag="O0305_000031"
     CDS             complement(30984..31919)
                     /locus_tag="O0305_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0273218.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKPAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDHKLRTYE"
     gene            32281..33810
                     /locus_tag="O0305_000032"
     CDS             32281..33810
                     /locus_tag="O0305_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0273219.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(34130..35083)
                     /locus_tag="O0305_000033"
     CDS             complement(34130..35083)
                     /locus_tag="O0305_000033"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0273220.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYEEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(35394..35501)
                     /locus_tag="O0305_000034"
     CDS             complement(35394..35501)
                     /locus_tag="O0305_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273221.1"
                     /translation="MSVCTPTIERIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            35656..36801
                     /locus_tag="O0305_000035"
     CDS             35656..36801
                     /locus_tag="O0305_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCY0273222.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            36827..37000
                     /locus_tag="O0305_000036"
     CDS             36827..37000
                     /locus_tag="O0305_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273223.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIIVKNNNLIKTLIQTIAGYTVF
                     AVGLKYLTKRKNK"
     gene            37157..37513
                     /locus_tag="O0305_000037"
     CDS             37157..37513
                     /locus_tag="O0305_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCY0273224.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYAAK
                     DTKHNQAVVLQQLLNT"
     gene            37711..39186
                     /locus_tag="O0305_000038"
     CDS             37711..39186
                     /locus_tag="O0305_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0273225.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(39500..40642)
                     /locus_tag="O0305_000039"
     CDS             complement(39500..40642)
                     /locus_tag="O0305_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCY0273226.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            41083..42660
                     /locus_tag="O0305_000040"
     CDS             41083..42660
                     /locus_tag="O0305_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCY0273227.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            42686..44191
                     /locus_tag="O0305_000041"
     CDS             42686..44191
                     /locus_tag="O0305_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCY0273228.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            44303..45514
                     /locus_tag="O0305_000042"
     CDS             44303..45514
                     /locus_tag="O0305_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCY0273229.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            45700..47961
                     /locus_tag="O0305_000043"
     CDS             45700..47961
                     /locus_tag="O0305_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCY0273230.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDTIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPISHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            47991..49175
                     /locus_tag="O0305_000044"
     CDS             47991..49175
                     /locus_tag="O0305_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0273231.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            49490..49597
                     /locus_tag="O0305_000045"
     CDS             49490..49597
                     /locus_tag="O0305_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273232.1"
                     /translation="MCVRTRLVSSSSARLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(49779..>51390)
                     /gene="coa"
                     /locus_tag="O0305_000046"
     CDS             complement(49779..>51390)
                     /gene="coa"
                     /locus_tag="O0305_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Staphylocoagulase, like its paralog von Willebrand
                     factor binding protein Vwb, is a coagulase and a virulence
                     factor. It induces clotting, not by being an enzyme, but
                     by activating host prothrombin to generate fibrin; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCY0273233.1"
                     /translation="YEYGDNIYKEAKDRLLEKVLREDQYLLERKKSQYEDYKQWYANY
                     KKENPRTDLKMANFHKYNLEELSMKEYNELQDALKRALDDFHREVKDIKDKNSDLKTF
                     NAAEEDKATKEVYDLVSEIDTLVVSYYGDKDYGEHAKELRAKLDLILGDTDNPHKITN
                     ERIKKEMIDDLNSIIDDFFMETKQNRPKSITKYNPTTHNYKTNSDNKPNFDKLVEETK
                     KAVKEADDSWKKKTVKKYGETETKSPVVKEEKKVEEPQAPKVDNQQEVKTTAGKAEET
                     TQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTVQGEIVQGPDFLTMEQSGPSLSNNY
                     TNPPLTNPILEGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQATETTEASQYGP
                     RPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSYG
                     ARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPT
                     YKKPSKTNAYNVTTHADGTATYGPRVTK"
BASE COUNT        16336 a         9492 c         8224 g        17338 t
ORIGIN      
        1 ctgtatgatt aattacacaa taaagagaag gaatataata tgaaaaaagt gtcaattaaa
       61 gatgttgcta gagaagctgg tgtatcagtt acaactgtgt cacatatttt aaatcataat
      121 gatagtcgtt tttccgcaac aacgataaaa aacgtacatg ctgtttcaga acgtttaggc
      181 tatgccccta ataaacatgc aaaacaattg cgcggcagta aaattcaaac tattggcgtc
      241 attttgccta gcttaacaaa tccgtttttc tcagcactga tgcaaagtat tcatgaccat
      301 aaaccatctg atgttgattt atgcttttta acatctacag caactgattt gtatgacaat
      361 attaaacatt taattgatcg aggtattgac ggattaatta tcgcacaata catatcatcc
      421 ccggacgccc taaataacta tctaaagaaa catcatgtac cttatgtcgt actggatcaa
      481 aatgaccatc aaggctatac agattttgtt cggacaaatg aatatcaagg tggacaactt
      541 gcagcacaac atttaataga actcggtcac aaccatatga taattgttgc accatatgac
      601 atgatggcga atatgtcgac tcgtgtcgct ggatttgtcg atactttgcg cgcgaatcaa
      661 ttgccagaac cacaaatcgt ccatactgaa ttatctaagc gcggtgggct aaccattgtt
      721 gatgacatca tggttcaatc tgccactgca atcttcgcta ttaacgatga actcgctatt
      781 ggcattttac gaggactaat tgaacatggc atcagtatcc cgaaagatat ctcattaata
      841 ggttatgacg acattgatta tgcagcgtac gtctcgccac ctttaactac tgtggcacaa
      901 cctataactg atattggcaa aacatcttta accttattac ttcaacgatt acagcactta
      961 gataaatcca ttgatatgat tgaattacca acgactttaa aaattcgtgc aacaactggc
     1021 tatcatcttt caaactaact acgtatcttc cgaaatatac tcatcattgt taggccctta
     1081 gcgttgcttt aatgctgagg gtttttaatc ataattattt tactaagaaa ttaaaataat
     1141 aatgtatgaa tttttaaata tgatttaaac gttttcagtt tttatgaaaa cgcatgcatt
     1201 ttacaaataa aaatggtacg atggcactgg taaaacgttt tactaaaaac aaatcatgag
     1261 gtgtataaca tgagcattgt tgcattactt atcgggttag gccccttaat tggctggggc
     1321 ttcttcccaa cagtcgcttc aaagtttggt ggtaaacctg tacatcaaat tatcggtgct
     1381 actgtaggta cgttaatctt cgctattata ttagccgtag tcacatcaag tggcttacct
     1441 actggaacca atttgctatt cgccttatta tcaggtgcag gatggggatt cggacaaatc
     1501 attacattta aagcgttcga attagtcggc tcatctcgtg ccatgccagt cacaacagca
     1561 ttccaattat taggcgcatc tttatggggt gtctttgcat taggaaattg gccaggcatt
     1621 ggtcataaaa tcattggatt tacagcttta gtcgttattc taattggagc gcgtatgaca
     1681 gtttggagtg aacgcaaaga agcaagtaac gccaaaaatt tacgtcgtgc agtggtactt
     1741 ctgttaattg gtgaatttgg atactggtta tattcagctg caccgcaagc aacttctatt
     1801 gatggcctaa ctgccttttt acctcaagca atgggtatgg taattgttgc agtcatttat
     1861 ggctttatga atatgaaagc agagaatcca ttccgtaata aaattacgtg gttacaaatt
     1921 atttcaggtt tcttctttgc atttggtgct ttaacatatc ttatttcagc acaacctaat
     1981 atgaatggtt tagcaactgg atttattctt tctcaaacat ccgttgtgct tgctacatta
     2041 actggtattt atttcttaaa acaacataaa acgtcaaaag aaatggttat tacaatcatc
     2101 ggcttagtac tcattttagt agccgcttct gttacagtat ttataaaata aggagtgtag
     2161 atgtcatgaa aaaatcagct attttaaatg aacatatttc aaaagcaatc gcgacaattg
     2221 gtcattttga tttattaacg attaatgacg ctggcatgcc aattccaaat gatcatcgtc
     2281 gtatcgacct agctgtaact aaaaacttac cacgctttat tgatgtctta gctacagtgt
     2341 tagaagaaat ggaaatccaa aaaatatact tagcagaaga aataaaagaa cataacccta
     2401 cacaattgca acaaattaaa caattgattt catcggaaat cgaaatcatt ttcattcctc
     2461 acgaagaaat gaaaagtaac ttagctcacc cattaaataa aggtaatatt cgtactggtg
     2521 aaacaacgcc ctactctaat attgcattag aatcgaatgt tactttttaa aagttataac
     2581 ttgaaaggag cgtacacatg accaacaaag ttgttatttt aggttcaacg aatgtcgatc
     2641 aatttttaac agttgaaaga tatgcacaac caggcgaaac attacatgtt gaagaagcac
     2701 aaaaagcatt cggcggaggt aaaggtgcca accaggctat tgccactgca cgcatgcaag
     2761 cagacacaac atttattact aaaattggca ctgatggcgt tgctgatttc atcttagaag
     2821 attttaaagt agctcatatt gatacatcat atattatcaa aacagctgaa gcaaaaacgg
     2881 gccaagcctt tatcactgtg aatgcagaag gacaaaacac catctatgtt tatggtggtg
     2941 cgaatatgac gatgacacct gaagatgtta ttaacgcaaa agacgctata atcaatgcag
     3001 actttgtcgt tgcacaatta gaagtaccca tcccggctat tatatctgca tttgaaattg
     3061 ccaaggcaca tggtgtgacg acagtattaa atcctgcacc agcgaaagca ttacctaatg
     3121 aattattatc attaatcgat attattgtgc caaacgaaac agaagccgaa ttgttatctg
     3181 ggattaaagt aactaatgaa caatctatga aagacaatgc caattacttt ttatctatag
     3241 gcattaagac tgttttgatt acgctaggta agcaaggtac atattttgct actaaaaatc
     3301 aaagccaaca catcgaagct tataaagtaa atgcgattga tacaactgct gcaggcgaca
     3361 catttattgg tgcatttgtc agtcgcttaa acaagtcgca agataactta gcagatgcta
     3421 ttgattttgg taataaagcg agctcactca ctgtacaaaa acacggcgcg caagcatcta
     3481 ttcctctact agaagaagta aatcaagttt aaatgaatca aacacagcta tgatatgaag
     3541 gtttagcata taacatgcaa cattcgtata tcatggctgt gcttttttat ctttataaaa
     3601 catcatctat tagaaataat tttccactaa acctatgctt gttgactcat gtttagttat
     3661 aaatgaagtg acaatttttt gtaatctttt taacttccaa attatcgcat ataaatatgc
     3721 tatattaatg ataataatta tcaattaaaa ggaggttatg ctatgtctaa agaagctggt
     3781 catacatttt tagctaaatt aggaaaaact cgtctacgcc ccggtggtaa agaagcaaca
     3841 gattggttaa tacaacaagg ggcattttca caagataaac aagtgttaga agtggcatgt
     3901 aatatgtgca caacatctat ttatctagct catacatatg gctgtcacat tcaaggcgtt
     3961 gatataaata agaaagcatt agaaaaagca caggaaaaca tttcagcagc aggtcttgaa
     4021 tcatatattc aagttcaaca agcgaatgct gttaaattgc cctttgatga caatcaattc
     4081 gatatcgttt taaatgaagc aatgttaaca atgttaccca tcgccataaa ggaaaaagca
     4141 ttacgcgagt actaccgagt cttaaagcct gggggtatct tgttaacaca tgatattgtc
     4201 atcgttaatg aatcacatgc cacacatgtt gttaaatcat tatctgcagc aattaatgtc
     4261 aatgtctcac cgcagacgaa acttggctgg ttagatttat ataatcaagc tggttttaat
     4321 catgtgcatt atcatactgg tccaatgagt ttaatgacac caaaaggttt aatttatgac
     4381 gaaggtattg ttggaactat aaagattatc aacaatgctt tgaaaaaaga aaatcgacca
     4441 atgttttgta aaatgtttaa aacgatgact aaattgcgta aagatatgaa ttatattact
     4501 tttgtcgcta aaaaagagca ctaaatataa tgccactaac tgtactttgt atctatgttt
     4561 gactatcact ttaatttctt tgtgacacta atcatctact taacaatatc gttatcgttg
     4621 attagtaagt catcaatttt ggttaaagac tttcataaac actcaaacat taacactata
     4681 catagttagt ggcattattt ttttcttaaa attttaacat cacgggattg gaacagaaat
     4741 gatattttcg caaaatttat ttctgtcctt ccccaacttg cattacctgt agaatttctt
     4801 ttcgaatttc tctgtgttgg ggccccgcca acttgcatta cctgtagaat ttcttttcga
     4861 aattctttgt gttgggggcc cgccattata acactgtagg tcctactata ttaatttatg
     4921 tctcgggctc taaccctaaa atacttataa tataccttta taatttatct ccatttgatg
     4981 ctatcacttg ttgataccaa tagaacgaat cttttttcaa gcgttccatc gtgccatgac
     5041 catcattatc tcgatcaaca tatattaaac catagcgttt cttcatttca ccggttgtaa
     5101 atgaaacaat atcaatgatt ccccacggtg tataaccgat taaatctaca ccatcttgat
     5161 caactgcttc tatcgctgct gtaatatgtg cttttaaata ttcaatgcga taatcatcat
     5221 gaatatgtcc atctaccact tcatcaactg caccaaaacc attttccaca ataaaaagtg
     5281 gtaactgata acgatcatat aacacattca atgtatatct taagccatct ggatcaatcg
     5341 cccaacccca atcactcgtt gcgatatgcg gattctccac agaatgattc aaaccaccgt
     5401 tgacgatgtt gttttcaact gtagtatcaa catcatgttt tacagccgta gacatgtaat
     5461 aactaaagcc aatataatca accgtgccct gctgtaaaat actgtcgtcc ccttcttgcc
     5521 atccaacatc atatcctttt cgtgccaaca tttttttagc atagcttgga taataacctc
     5581 tcacttggac atccgggaaa aagaaacgta agcgattcgc aatttgtgct tccatcatat
     5641 ctttcggatg acacgaatat ggataaatgg gtacatgtga aatcattgtt ccaatcttaa
     5701 acttcggatt aatctcttta ccaagacgaa ctgctaaagc actggctaaa agttcatgat
     5761 gtgctacttg atacaagact tcttcaggat tatcattttc tgtcaatgct accccagaat
     5821 tcgtccataa aaagatagga tttgatgtgt ccatctgatt attaatttca ttaaacgtca
     5881 tccaatatgt aactttatct ttatatcttt caaatacaac acgcgcaaaa tgcacaaaat
     5941 aatcgacaac ttctctattt ctaaatccac cataatgttt cgctaaatga agtggcatct
     6001 caaaatgtga taacgtcaca acaggttcaa taccttgtgc aattaattca tcaaagatac
     6061 gatcataaaa ggcgagtcct tcttcatttg gcacatcttc atccccattc ggaaagatac
     6121 gtgtccacgc aatcgacgta cgtaaacatt tcaatcccat ttctttaaac aaggcaatat
     6181 cttccttata acgatgataa aaatcaatac cttcatgatt tggataatag tgattgggat
     6241 ctatagattc tgtaatctga cgtgctttgc catgtgcacc actcgtcata acatcaatta
     6301 cacttaaccc tttaccacct ttatcatatc caccttcaaa ttgatttgcg gcaagagcgc
     6361 caccccacat gaaattttgc ggtaattttg tcattattac aacacactcc tagtttttaa
     6421 atattttaaa aataacatct tcagtcgtaa tgatttcatt cattgtcagt tctacttttc
     6481 ctaaatcggc agagttgcta ataacgacaa taacgtcagc attgtagcct tgttgttgta
     6541 tatattgctg gtcgaattgc aacaacgttt gccctgcttc aacatgatca ttttgcttta
     6601 caaagcactt gaacccttta ccttccaagt caactgtatt caagccaata tgaatgacta
     6661 tgtccacacc gtcttctgat tgaataccaa ctgcatgctt agttggtaca atcatagata
     6721 ttaaaccatt gaacggtgcg attactttgg attcttcatg agctttgatt gctaagcctt
     6781 cacctaccat tttctctcta aaaattgaat ctcttacttc tttaagtaaa atacgacgac
     6841 cagcgattgg tgctttcact aaaattggtg ttgttacttt tacttgaggt gtataacttg
     6901 tatcttgact attaatattg tcagtattct cactctcttc gacattagca ttcttaatta
     6961 atcgctgtaa ttgctgatac acttcatcga catttccatt taattttatg actaactgcg
     7021 tatcattttc tgctacagat gtcacatcaa ctatttgttt cacttcattt gctgtagaag
     7081 gaattgtatg ttgcatatga attgtaatgc acttcgtatc atacgttgca tcaacaatgt
     7141 tttcgacacc acctacagct gtaagaatgt cttgggcttg ttctttatat ttattgctca
     7201 taaatattac cccctcaact tgatagtaga atgaaaatgt atttacttac ctccaattat
     7261 aattgtaccg gttcaatttg taaacgccga tacaattata atattttgtg ctataataat
     7321 tacagacaaa gtgaaacgag gacagaatat tgttaaagta tgaacatatt gctaagcaac
     7381 ttaatgcgtt tatacatcaa tctaatttca aacccggtga taaattgcca agcgtgacgc
     7441 aattaaaaga acgttatcaa gtaagtaaga gtactatcat taaagcatta ggcttattgg
     7501 aacaagatgg tttgatctat caagcacaag gcagtggtat ttatgtgaga aatattgctg
     7561 atgccaatcg tatcaacgtc tttaagacta atggtttctc taaaagttta ggtgaacacc
     7621 gaatgacaag taaggtactt gtttttaagg agattgcaac gccacctaaa tctgtacaag
     7681 atgagctcca attaaatgca gatgataccg tctactattt agagcgatta agattcgtgg
     7741 acgatgatgt tttatgtatc gaatattctt attatcataa agaaatcgtg aaatatttaa
     7801 atgatgatat tgctaagggc tctatcttcg actatttaga atcaaacatg aaacttcgta
     7861 ttggtttttc agatattttc tttaatgtag atcaactcac ttcaagtgaa gcttcattac
     7921 tacaattgtc tacaggtgaa ccatgtttac gttaccacca gactttttat acaatgactg
     7981 gcaaaccctt tgattcatct gacatcgtat ttcattatcg tcatgcacag ttttatattc
     8041 ctagtaaaaa gtaataaata cataaaaacg tctatatccc agttataaac tggagtatag
     8101 acgttttttt acgataataa caatggctca aattgctatt atcttgctta ggtttttcgt
     8161 tttagaagaa tattgctaca aagacaggca caactgctac aacaactaca ccaactaaca
     8221 ctaaagctat acttgccatt gattcttcta caggtcctaa ttctttggct ggtgctacac
     8281 ctaatgtgtg accacttgtt ccaagtgcta atcctcgggc aatagggtta gtaattcgga
     8341 aaagctttaa gaatttatta cctagggcat aaataatgac accatttaaa ataactgcta
     8401 atgatgttaa ttcttttata ccaccgatac cagctgatac tggtaacgca atcgctgtag
     8461 ttgctgcttg aggtaacatt gataaaataa catcattggc aaattgtgct aacttcgcaa
     8521 aagttaaaat aattaataac gctacaactg taccgatacc aatacctccg atgatacgat
     8581 gccaatgttt aacaagcact tcacgctttt tatataacgg aatcgcaaaa cagattgttg
     8641 ccggttctaa gaagaagtaa ataatgtctc cacctatttt gtaagtctta tacggaatgc
     8701 ctgttaaata gaggaaggcc acaccaaata ccatactgac aaatagcggt gcgaataaga
     8761 agaaacgatt agttttttca aataatatgg tcgctaagaa aaatggtata acggataaca
     8821 gtattccgaa gtaaggtgtg tttagtgcta agtggttaat catgagcttg tgcctcctct
     8881 attttgatct tttttgtgac tttgtcacct ttagatctcg aagtaacttt cataataatt
     8941 tgtgtgacat agccagtaca aataagtaat agtattgttg agacgattat tagtccaatg
     9001 attaaaaatg gtgcttggct aatgacacct aaagagttaa caactgagat accggctggt
     9061 acgaagagta agccaatgtt atttgttagt gtcgttccta ctttttcgac ttcgcctaac
     9121 ttaacagcac cagtacataa taatacaaat aataatacta aaccgattac tgatgcaggc
     9181 ataggaattg gcataaatga ttcaattatt ttcgatacaa agagtactaa agcaattaca
     9241 atgacttggt gaaaaaagtg tgctggtttt gatgcgtctt tttgttgttt cacgaccatt
     9301 gcctcctacg tttgatttaa ctaaagtata gatggctcac ttcgatttgc gtgattttta
     9361 gtccgaaata caaaatatca taggtaaaat gcataaaaaa aaggattact gttaaagtaa
     9421 tcctatcgac gctttaaaat ctttcataaa tgaacgtcca acttgcatct tgacaccatt
     9481 tgtcaatatt accatataag tatagttaaa ccattgttgc acttctttaa tgtgtttcgt
     9541 gttaataata tatgaacgat gaatacgtat aaaataagtg ggattcaatc gtttttcata
     9601 acgattcaat ggctctgttg tttcgtattt atgattcgtt gtatgtatgg ttgtaatacc
     9661 attatgtgtg ccaatcccaa taatattttg ttgctttaac atgtgaattt tatcgtcaat
     9721 ttcaacaggt aagctttgat caaaattcgc cgacatatca ttcgcaattg cacttgcgtt
     9781 attatcatct ttggctttag tcgcacgcac tttattgact gcttgttcaa tacgtttttg
     9841 accaaacggt ttcaaaatat agtctgtcgc atttaattca aatgcctgta ctgcgtattg
     9901 gtcatgtgca gttgcaaaaa taatcgcagg tggctctttc atcttttgaa tcttagctcc
     9961 taattcgatc ccattttcat ccattaaatt gacatctaaa aatataatgt catattgatt
    10021 gatcagtagt gcttccaatg tttcttttac attttctgcc tcattaattt cttcaaaacc
    10081 accaatttca tttaataaat atgttaattc attacgtgct aatggctcat catctatgat
    10141 taatgctttc atatttattc ctcctcttgt ctttcataag gaagtacaca ccaaaaagtg
    10201 gtaccgctcg atgtcgattc aaattgtaat gctgcggatt ttccaaataa tccttttagg
    10261 cgtaagttta aattttctaa agcactacca gttccagact ctgattctac agatgtttct
    10321 cccaacaaat gcattttatc tttagaaata ccctgaccat tatcttgtac aataatacgt
    10381 acatgtgttg cagtttcttt aatcactgac acgtcaatat cgttaccttg ctttcgattc
    10441 gtaaacgcat gtttgatggc attttcaact aaaatttgaa ttaaaaatgg tggtacaagc
    10501 acatcgcgat atttgtcttc aacattaata ttgatattaa atcttcctgg ataacgtgct
    10561 tgttcgagtg ataagtatgc acgcacttga cttaactctt tatctaaagt aatcgtatgt
    10621 tgcttagagc cttgtaaatt cgctctgaaa aaataactca attctaatag taactctcgt
    10681 gccttttcgc tatttattct aactaaagct gagatcgtgt taattgaatt gaagaaaaaa
    10741 tgtggactca cttgtgcctg taatgactta atctcagcat ctttcaataa cttactttgc
    10801 gtttcggctt caccaagttc aatttggcta ctaaaaatat ttgccaatcc ttctgcaagt
    10861 tgacgttcca caaaagttaa atcattaggg tttgtaaaat acatcttcaa tgtaccgacg
    10921 atagaaccat gcatctcaag tggtatcacg atagctgctc taagcgggca attcggatga
    10981 ctacaaccaa tctcttcttt agtatgaact tctttcaact ttcctgattt caatacatct
    11041 ttagacagac ttgttaatat ttcatttgtt ggtatgtgat gatcactacc tgcacctaca
    11101 tgcgataaga tttcattttt gcttgtaatt gctacggcag atactttcat taaattttta
    11161 ataatcatcg caatttgctg tgccgattct ctattcaatc cttctttaaa atacggcaat
    11221 gtctggttca tcaattgcag tacatcatgt gtttgaacag ccttcatttg ctcctcttgc
    11281 tttaatgttg aaatgataat agacataaaa atcgccgtac caacgctatt aacaataatc
    11341 attggtagtg caattaatga tatgaggtca accgcatatg ctttgtcgtg ggaaaatgtt
    11401 aaaatgctca acatttgaat catttccata acaattccaa tcatggcact tttcgcaata
    11461 ctcgggtaac gcttgcgtct ttgagcttgt aagccaaaat aaccagcaat tataccaata
    11521 aatatagatg agataagata aacttgtgca tccgccccac ccatatacac tctgaaaata
    11581 cctgaaataa cgccaacaaa tagacctaca aaagggccac caactaatcc tgcgacacct
    11641 atcgttaata cacgtgtgtt agctaaagat acatcatcat ctaaacggaa gtacacactt
    11701 cctgacaaac tatgttgatg atcgatgacg ataccagtta aattagacat taaggcaaac
    11761 aaactgaaaa taatacataa ttgccaacgt gctttccatg tacgtcgacg attcattaag
    11821 tttttaaaat atggaatatt catcaacaca taggccaaaa taataattaa acctacacgc
    11881 tcaagtaata acattgttag cgatagcacg atagcacctc agtgaattat aattatgttt
    11941 attttatcat aatagtgcat gtaaaattca tgaataaatt attaaaaaaa cacgctacta
    12001 tttaattatc tataccatta aattttcata tcaattttta tgctaagttg accatttttc
    12061 ttttcaaata atgctgttta tgtcactatt atgactttta tacatagcga aaaactgctg
    12121 gacttatttt tgccaacagt ttctaatcgc gttatttatg atactttttt aaataatacg
    12181 tgattctcta aatgtacgtg ttcatgtgtt aacacttcaa gtgcttttaa tctctgataa
    12241 acaagtcgcc aagtaccaca cgcttctatc ggaggttcat aactagatgt taattcgctc
    12301 atttttacta acaattctcc cgttgcaatg tggtctgaaa ctaaatcatc tatcacagta
    12361 ttaatatcgt ctactacctc accttgctca tatttaatga gttttggaaa atcgacatcg
    12421 tcttcttttt gcatatgctc taacatgcca tttttaaatg tatcgtatgt ttcttttaac
    12481 tcaactaaat atggatgatt aggtccatgt actttcgata acttcgtcac ataaggtgtt
    12541 aaatttttaa attcttctct tagaggttca tgatatgctg attgaatata ttgaataagt
    12601 gatgaaacat ttaaaaattt aggatttaac gaacctggtg tattcgtttg ttcaacgtcg
    12661 ttgagacgct gtaataattc gttcaaatct acatttttct tttctaaggc tgctgcttct
    12721 atacttactt gtccgccaca acaaaaatct attcccacac ttctaaaaat atccgctgct
    12781 ttcggataat cagttactac atctgctact atgtcatttt tatttatcat tatagataac
    12841 ctcctcattt ccagtgtata caatttaatg tatatgaaaa tgaaaatcag ggaaatgtca
    12901 taatcatgtc ataaacagca aaataaagcc ctcaaatcat gttggctcac aatgatttga
    12961 gggcatttat tatttattta gtggataagt gatatgtgca gtgtttaact taacaatatt
    13021 tttgaagtct cgctttttaa tatctttgct aagtaagatt tgcacttcgt tttctgatgt
    13081 ttggcttaac attcccatca ccatgtcgtc ttttagtacg acactagcca taccttcttc
    13141 aaatacataa ccacgatcca gaccagttaa aatgattttg tttttacctt ttaacttcaa
    13201 tgagattgaa tccgttaatg taatagctgt aaagtataac gaaatgttat taaagtcttt
    13261 tgtgaaatac ggtgttaatc tataaaaatc attattcaaa tgaattaatg cacttgtttc
    13321 tgcagtatat gcatatttat tacgttgatt accaacacgc acttcaaata cttccgtacc
    13381 tatttggact tctaagaaaa catcccaaat tttttcttta ggtaaatacg ctttaatatc
    13441 ttctaacata atgttaaaac gatattgttt attcgcattg acacttaaag gtacatacat
    13501 tgtgtgcttc ttgtttcgtg aatgtaattt aagtctgaca tcataatgtc cttcgccaat
    13561 cataaattca tttaacgtta gctctgtaac aattttatga ttatcaaatt taaatgctgt
    13621 cattttcact tttggcttag cgatatcaat gcctttaaag tatggttcgc cttcattgaa
    13681 ttgaatgttt aatttaccat ctacaatatc aaaacggtag tattgacctt gacggtaact
    13741 ttcctccaca gttctatagt tgtctatatc tttcgctctc gcgtagtgca acaatggtcg
    13801 taatttactc atcaccaatg catctacacg ttctggaact gcttgtataa agtctcctag
    13861 agcattaatc cattgtggtt gatcttctag tttaacttta agtgagaagc cattcgtacg
    13921 agaaaaacta aaatgacgat ttaaaaattc tgctaagatt tgatttttat gggcttcttc
    13981 taaatctgca tttaatattt ctacagcaat caatctcatg acttcataaa agtcttcagg
    14041 tgaaacatac gcactactca tatgttcacc ttcacgcttt gtagcataat aatacgcttt
    14101 atcacttaac acactgattc gatttgcatt taaatatgct ttcattgtaa ataattgatc
    14161 ttctgcactc tttaattctt ctggaaaata aatgtcatta tcttttagta atgctgttct
    14221 atagatttta gtcgggctta aagtatagat aattcttgaa tttaacaaag taacttcagg
    14281 tgctgtttct ttaaacatag attgtggtac accacgacca ttaacacctt tcattttaat
    14341 caataagaca tctgagtgat gttcatcaat aaatgctgct gcatccttta aagtctcttt
    14401 gtttatatag tcatcggagt ccacaaataa tacaaattca ccttctgctt gttttaacgc
    14461 cacatttcta ggtttacctg gaccaccaga atttgtttcg agttgactta ctttcaagtt
    14521 caatttattg cgatactttt caactatttg taacgtttga tctgttgaac agtcatcaac
    14581 tacaaccact tcaaattcag ttttcggaaa atcttgtttc gcaaggctat taagtaattc
    14641 tgttatatac ttttctgaat tgtatgttgg aactattact gaaaatttca tcattatacc
    14701 tctcccactt tgactactat ataaacttag ctaccaaata aatttctgac taaacgctca
    14761 cttgatcggc catcttgata tttaaaatgt ttatctaaga atggaatgac tttttctcct
    14821 tcataatctt cattgtccaa ggcgtccatt aatgcgtcaa atgattgcac aattttacct
    14881 ggaacaaatg attcatatgg ttcataaaaa tcacgcgtcg taatataatc ttctaaatca
    14941 aatgcataga aaatcattgg ctttttaaat actgcatatt catatattaa agatgaatag
    15001 tcactaatta ataaatctgt tatgaacagt atatcattaa cttctctaaa gtcagaaacg
    15061 tcaacaaaat attgtttatg tttgtctgca atattaagtc tatttttcac aaatggatgc
    15121 attttaaata atacaaccgc gttatttttt tcgcaatatc ttgctaaacg ttcaaaatca
    15181 attttgaaaa atgggtaatg tgctgtacca tgaccactac ctctaaatgt tggtgcgaaa
    15241 agaatgactt tcttaccttt aataattggt aattcatctt ccatctcttg tttgatctgt
    15301 gtcgcataag cttcatcaaa tagtacatca gtacgtggaa cacctgtagg cactacattt
    15361 ttctctttaa taccaaatgc ttcagcgtag aatggaatat cggtttcaga tgatacataa
    15421 gcttttgtat agctacgatg atttaatgaa tcaataaatg gtccaccctt tttaccagta
    15481 cgactaaagc caactgtttt aaaggcacca acggcatgcc atacttgaat aacttcttga
    15541 gaacgtctaa aacgcactgt ataaatcaat gggtgaaagt catcaacaaa gatgtagtct
    15601 gccttcccaa gtaaatatgg caatctaaac ttgtcgatga tgccacgtct atctgtaata
    15661 ttcgctttaa aaacagtgtg aatatcatac tttttatcta aattttgacg taacatttcg
    15721 ttatagatgt attcaaagtt tccagacatc gttggtctag agtctgatgt gaacaacacc
    15781 gtattccctt ttttcaagtg gaaaaatttc gtcgtattaa atatcgcttt aaaaataaat
    15841 tgtcttgtat taaatgattg tttgcggaaa tacttacgta attctttata tttacgaacg
    15901 atataaatac ttttaacttc cggagtcgtt acaacaacat caaggacaaa ttcattaaca
    15961 tcgctagaaa tttcaggtgt aacagtataa accgttttct tcgaaatgcc gccttttcta
    16021 aattctttta ggtaagtctg caataagaaa ttgattttac cattttgtgt ttctaattcg
    16081 ttgtattctt cttcttgttc tggctttaga ttttgatatg catcattaat cacatctggg
    16141 tttaactgtg caatataatc aagttcttgc tcattcacta ataagtactt atcttcaggt
    16201 aagtaataac cattatctaa gatagctaca ttgaaacgac aaacgaattg attcccatct
    16261 attttgacat cattcgcctt cattgtacgt gtctcagtta aatttcttaa tacaaaatta
    16321 ctatcttcta aatctaggtt ttcactatgt ccttcaacga ataactgaac acgttcccaa
    16381 tagattttat ctatatatat cttactttta accaacgtta attcatcctt ttctatttac
    16441 ataatccatt ttaatactgt tttaccccaa gatgtagaca ggtctgcttc aaaagcttct
    16501 gtaagatcat taattgttgc aatttcaaat tcttgacctt ttaacaacgc taatttatct
    16561 acaatatctg ggtattgaat gtataagtct acaacatctt ggaaatcttt tgaaccactt
    16621 cgactactac caatcaacgt tagtcctttt tccaatacta gacgtgtatt aacttctact
    16681 gggaactcac ttacacctaa cagtgcaatg cttccttctg gtgaaatgta atcgatcatt
    16741 tgatttatgg ctgattgact accgcgacca cccacacact caaatgcatg atcaaatgtt
    16801 aagccttcag gtattttatt aataaagaag acatcatcaa caaatgagaa gtgactcaat
    16861 ttataatctg ttttaccaaa gacatatatt ttagactctg gatataattt acgtaataaa
    16921 atggctgtaa tgtaacctaa gttaccatca ccccaaatac caaatgtatt tttatttgaa
    16981 atagatttct tttcaaaacg acgaatagca tgcaaactta ctgttacaag ctctgtatat
    17041 gaaataatac ttaaatcaat atcatcaggt agtggtacag ctctatcatg atttagcaac
    17101 acaaaatctt gcataaaccc atcatgtcca cttgatctga agtagctcga ttttaaatag
    17161 ttttcagcaa tgacatcgtc tttttctgtc ggcgtattcg gtaccataac tacttttgta
    17221 cctttattaa acacaccttt actgtcaaat acgacctcac caacaccttc atgaattaaa
    17281 gacattggca atttctgaga taagacattc tcatcacggc taccagtata atatctttga
    17341 tcggcagcac aaattgacat atataaagga cgtacaatga catagtcact gtaaatatct
    17401 acgttgttat acgtaacttc aaattgtcta ggtgcaacta attgatatac ttgattaatc
    17461 atcggcaata ccacctcgaa taatagcatt cgctactttt aaatcgtaag gtgttgttac
    17521 tttaatgtta tataactcac cttttacaag tcgaaccggt ttgtttgttt ctacaataat
    17581 cttacaagca tcagataaaa tactcttttg ctcatcactc aactgtgcat agctttcttt
    17641 taataaatta atattaaacg attgaggtgt ttgaccttgg tacatttcat tacgcactgg
    17701 aattgcatca atcgtttgat tatctttaga tgtaacaatc gtatctatag catcaatcac
    17761 tgtatctact gcaccgtact ctaaagcagc ttgaatattt tctttaataa tacgatgcgt
    17821 taaaaatggt ctaactgcat catgtgtcac aatgacatcg tcatcgttaa taccatttgt
    17881 tgattcaata tgtttaacga tattcataat tgtatcgtta cgatcgctac caccttgaat
    17941 gacttcaatt ctttcatcag aaattttgaa ttttctaagt gtatctttcg tatgcgtcat
    18001 ccattgttgt ggcgtcgcga taataatttt ttcaaaatca ttaattaaaa taaatttttc
    18061 taatgtatgg attaaaatcg gtttgttgtc taaatctaaa aattgtttag gtaaaggtac
    18121 gttacccatt cttgagccta tacctccagc tagaatacca gcgtatttca taaaatactt
    18181 cctccattca actatatcta tatttaatta tttaaatttc gttgcatttt ccaattgaaa
    18241 actcatttta aaatcaaaac tctaaatgtc tgtgtattac ttaaaattat acatattttg
    18301 cttatatttt agcatatttt gtttaaacct atattacatt atatcagacg ttttcataca
    18361 caaataataa catacaagca aacatttcgt ttattattta tatcacttaa ctaattaatt
    18421 tataattttt tattgttttt aagttatcac ttaaaaatcg tttggcaaat tcgttgtgac
    18481 gcttgtccat cttctaatga acagaatttt tgataaaata ccgttcgtgc ttcaatatac
    18541 tcatttgcag tctcatcgat ttgttttaat gcatcaatga gtgctgtttg attttcaaca
    18601 attggacctg gcaactcttt tttataatcc atgtaaaaac ctctaagctc atcgccatat
    18661 ttatctaagt catatgcata gaaaatttgc ggacgcttta atacaccgaa gtcgaacatg
    18721 acagatgagt agtcggtaac taacgcatcg ctgattaagt ataaatccga aatgtcttca
    18781 taatctgaaa cgtctttcac aaaatcatca tgttcatcaa tacgtgtcac aactaaataa
    18841 tgcatgcgta ataaaataac ataatcatca tccagcgctt gacgcaaagc ttctatatca
    18901 aagttaacat taaattgata tgaaccttct cgaatcgctt catcgtcacg ccaagttggc
    18961 gcgtacataa tcactttttt atctaatgga atatttaatc ttgtcttaat accattaata
    19021 tattcagtat cattgcgttt atgtgataat ttatcatttc ttggataacc tgtttccaaa
    19081 atcttatctc gactaacatg aaatgcattt tgaaatatcg atgtcgaata tggattaggt
    19141 gacactagat aatcccaccg ttggctttct tttttaaagc catcttggta attttgagta
    19201 tttgttccta gcattttaac gttactaata tccaaaccaa tcttttttaa tggcgtgcca
    19261 tgccatgttt gtaagtacgt cgttcgcggt gatttatata accaatctgg tgtacgtgtg
    19321 ttaatcatcc acgctttcgc tcttggcatc gctaaaaacc atttcattga aaactttgta
    19381 acatatggta cattgtgctg ttggaatatg tgttcatatc cttttttcac accccatatt
    19441 aattgggcat cgctatgttc agttaagtat tcatataatg ctttggggtt gtcgctgtat
    19501 tgtttaccat gaaagctttc aaaataaatt agattcttgt ttggcaattt tgatagtaat
    19561 ttaaaagtcg tatatatact atgttctatc aattttttaa ttgtattttt aatcatgtcg
    19621 tacctccgac gtgtttttgt aattatatta atatgtatga gcaagctcat tgtaaccatg
    19681 cctattatag catttcatca taaaatacat ttaaccatta cacttgtcgt taattatcat
    19741 acgaaataca tgattaatgt accactttaa cataacaaaa aatcgttatc cattcataac
    19801 gtatgtgttt acacatttat gaattagata acgattggat cgattatttt attttacaaa
    19861 atgacaattc agttggaagg tgattgcttt tgattgaatc gccttatgca tgaaaaatca
    19921 aaaggttatt ctcattgtat agtcctgctt ctcatcatga catgttgctc acttcattgt
    19981 cagaaccctt cttgaaaact atgccttatg actcatttgc atggcaagta atatatgcca
    20041 acattagcgt ctaaacaaat ctttgactaa acgttcactt gagcgaccat cttgatattt
    20101 aaaatgttta tctaagaatg gcacaacttt ttcaacctca taatcttcat tgtccaaagc
    20161 atccattaat gcatcaaagg actgtacaat tttacctgga acaaatgatt caaatggttc
    20221 atagaaatca cgcgtcgtaa tgtaatcttc taagtcaaat gcatagaaaa tcatcggctt
    20281 tttaaatact gcatattcat atattaaaga tgaataatca ctaatcaaca agtctgtaac
    20341 aaagagaata tcgttaactt cacgatgatc tgacacatcg ataaagtatt gtctatgttc
    20401 acgtgaaata ttaagtctat tttttacgaa cggatgcatt ttgaataaca caactgcatt
    20461 atgcttctcg cagtatcttg ctaaacgttc aaaatcaatt ttaaaaaatg gataatgtgc
    20521 cgtaccgtga ccattacctc taaatgtcgg tgcgaataga ataactttct taccttttat
    20581 aattggcaat tcatcttcca tctcttgttt aatttgtgtt gcataagctt catcaaatag
    20641 tacatcagta cgtggtacac ctgttggtac aacattttct tctctaattc caaatgcttc
    20701 agcataaaat ggaatatcgg tttctgatga aacatatgct ttcgtgtaac tacgatggtt
    20761 taatgaatcg ataaacggac cacctttttt acctgtacga ctaaagccaa ctgttttaaa
    20821 ggcaccaaca gcatgccaca cttgaataat ttcttgtgat ggtctaaagc gaaccgtata
    20881 aattaatgga tggaaatcat caacaaaaat ataatcggcc ttaccaagta aatatggcaa
    20941 tctaaacttg tctctccatt tgcgtctatc cgtaatattc tccttaaata ccgttttaat
    21001 atcataatca aaatctactt tttggcgtag taactcatca tatacatact tgaaattccc
    21061 tgataaattc ggacgcgaat ctgatgtgaa taatattgtt ttgcttcttt taatatgtag
    21121 taactttgta atattaaaaa tagctttaaa taagaaactt ctactttcaa atgaagcttt
    21181 atgaccttgt ttatgaagcc agtgtgcact tgtcgcaatg acccctgatt tctcttgagg
    21241 taaggtgatt tcaatatcaa atacaaattc gttaacgtca cttgctattt ccggagtaat
    21301 ggtatagacc gtatgttgtg atacgccacc tttacggaaa actttagcat catacgctaa
    21361 taaatagttc acttttccat tttgtgtagt cagctcattg tattcttcaa tttgctcttc
    21421 atttaatgcc gcataagctt gatcaataat atttggatta agttgtccaa tatactctaa
    21481 ctggtcatga taaacaaata aatatttatc cattggtaaa taatacccat cgtctaatat
    21541 tgcgacgttg aatctacata taaatgtatt accgtctatt ttcatttcat ttgcttctaa
    21601 tgtttttgtt tcggttaaat tcctcagaac aaattgtcct gatgttaaat cgacaccttc
    21661 actatgtcct tcaatatata actgaacacg ttcccagtat atgttatcaa tatgtattgc
    21721 ttgtttcgtt tttgtcattg ttattccccc atcggtttat taaatcgtcc atttcaatac
    21781 tgtttttccc caagatgtcg ataaatccat ttcaaacgct tggacgatat cttgcatcgt
    21841 acatacatta atttcatgtc cttttaataa tgctaacttt tcaactatgt ctgggtactt
    21901 acgatataaa tcaacaactt gctcaaaatc tttagagccg cttcgactac taccaatcaa
    21961 cgttaatcct ttttcaagta ctaatcgtgt attcacttcc acgggtaatt cacttacgcc
    22021 taacaaagca atactgcctt ctggtgaaat atgttcaact atttgttgaa gtgcaacttg
    22081 acttccttta cctccaacac attcaaatgc atgatcaatt ttaagatcat ctggtatttg
    22141 atttactgta aagatgtcat ctacaaatga aaaatgactt aatttatagt ctgtcttacc
    22201 aaatacataa gttttagctt ctgggtacaa cttacgtagc aaaatagcag taatataacc
    22261 taagttacca tcaccccaaa taccaaagct ggttttcaaa ggtatagatt tacgttcaaa
    22321 tcgttgtata gcatgataac ttactgacac taactctgtg tatgaaatcg tactcaaatc
    22381 aatgtcatta ggcagcggaa cgatacgatc atgtgccatc acaacgtagt cttgcataaa
    22441 accatcataa ccactagatc taaaataact agaggctaag taattctccg caataatatg
    22501 atgttgctct gtaggtgtat tcggtaccat tactactttc gtaccttttt caaatacccc
    22561 tttactatca aatacaactt caccaacagc ttcatgaact aatgacattg gtaatttttt
    22621 gcgtagtaca ttttcatctc ttcgacctgt gtaatacctt tgatcagctg cacaaataga
    22681 caagtataaa ggtcttacga tgacatgatt accataaata tcaacattat tatatgtgac
    22741 gtcgaactgt ctcggtgcaa cgagttgata tacttgatta atcatcggca atatcacctt
    22801 gaataatggc atttgctact tttaaatcat acggtgttgt cactttaatg ttgtatagtt
    22861 ctccacgtac caatttaact gcatgtccag attcgacaat gattttacat gcatctgata
    22921 agatttcttt ttgttcacta cttaaggcgc gataactatc ttgtaataat ttaatattaa
    22981 atgattgtgg tgtttggcct tgatacattt cattccttac agggatactg tgtatgttct
    23041 gtttatcttt agacattaca atcgtatcaa ttgcttcaat gactgtatct actgcaccat
    23101 attttgctgc tacttcaatg ttctctttaa taatacgttg agttaaaaat ggtcttacgg
    23161 catcatgagt tacaatcaca tcatcattat taattccatt tacattgcga atatggtcga
    23221 taatgttcat aattgtttca tttcgatccg taccacctgc aactactttg acacgttgat
    23281 ctgtaatgtt atattttttt aaaatatcct gtgtatggga aatccactgt gctggcgttg
    23341 cgataataat ctcattaaat tcactcacta aaatgaactt ctcaattgta tggattaaaa
    23401 tcggtttatt atcaatatct aaaaattgtt ttggtaatgg cacgttcccc attctcgaac
    23461 caatacctcc tgctaaaata cctgcataaa tcatgttgtc ctccattctg tcattacatc
    23521 atttccattt atacattact gacctatgcc cgcacataag cctaacctat tgctcacttg
    23581 cctcttttat taatccaaag atagttgtca caatagtgtg ataatttttt ataaaaatgt
    23641 atttttgtaa ctgaccattc taagttgttt tgccatgcag ttaatcatta actctgacga
    23701 tattaaattg ttaaaggtat taatgtttac tctttttcaa attcattatt actgccatca
    23761 ttttaccata tattataata aatttatctt attaagtggc tgtacttgat tttcacttta
    23821 aaaattatca aatattgcca tctcatttta agtatacaaa atgcaaaaca accgattcac
    23881 aagcatattt cacacaagta aaccggctat ttatcaacgt atattcgaag atgaattatt
    23941 tcgatagtat ctatagacca gacggcattc gcactttcat agctataact ataccagcgt
    24001 tttcgtcctc aaaggtgcat actaataaat cgtaaacatg actttatcaa atcgttcttt
    24061 cttgttaact aatttatcaa atgtctccgg gcctttttct aacggtaaaa aatgagaaat
    24121 aataggcttt acattaatat ctttcgtctt catataatgt aaggttgccg tccactcttt
    24181 gcccggaaaa ttactggaca aacagttcca agagccacat actgtcaact cgttacgcag
    24241 aattttttca aaatgaacgc gatcaatctc aatatcatca tatggtattc cgagtaatac
    24301 cacctcgcca ccttttttag gtagcgtcaa tatttgacca atcgtaactt tagcacctga
    24361 tgattctata gctaaatcga tttgattggc gtaatgattt tcgatgaatt tctcaagatt
    24421 ttcttctttt gaattgattg tttgatgtgc gcccaatgat gttgcaatat ctagtttatg
    24481 cgcatctata tctatagcga tgatatgtgc agcaccaaat attcgtgccc attgaatagc
    24541 taacaaacct atactgccac accccattac tgcaacagtc ataccaggtt gtatattcga
    24601 tttataaaac ccatgcgcaa caacggctga tggctcaacc attgctgctt caatgtaatc
    24661 aacattgtct ggaaccttta aaacattttg cgctggcaat ttgacatatt ccgcgaacga
    24721 tccaggttca tatgagccaa tgacgaataa cttttcacat cgtgcatatt caccttttaa
    24781 acaatactcg cattgataac aaggtattgc tgggcaacct gtcactttgt cgcccacatt
    24841 aacatgcgta acatcacttc caatggcatc tactacacct gaaaattcat gaccaaatgg
    24901 cataccttta atgtatggcc ccattttttt gtatcgtgac gtgtctgaac cacatatgcc
    24961 agtcgctcgt actttaataa taacgtcatt cgcactttca atgactggct tttcattatc
    25021 ctcataccgt aaatcttcca cgccatataa tttcaatgct ttcacttgta aatcacctca
    25081 aatttgattt aattcacaac ttttttcttt ttaaaaatac ctgtcgcaaa ataacctgca
    25141 atgacaatgg aattacttac gagtaaatgt tccatataaa aatcagtgat ttgtcttaat
    25201 ggcccaagca taaaagttag cattgattct accatattaa ttctctcctt cattttcatc
    25261 taaatcgagc ggcttaagta atactttaat accttcgccg gattttaaat gttgatacgc
    25321 ctcgtcccat ttcgaaatat catatatttt tgtcaccaaa gcttcagcat ttactaaacc
    25381 atccgccata agttgcaatg aaggttccca atctgctggc ttttgacttc tactaccaac
    25441 aactgttatt tctttttgaa tcactttttc catatcaaat ggaatttcag catccttaaa
    25501 aatacctatt tgactgtaga aacctttttt gcgtaaaata tccaaacctt gtcgtgctgc
    25561 tggaactgca cctgaacatt caacaacaac atctgcaccg taaccgtctg taattccatt
    25621 gatatacgtt tttaagtctg tttgttgtaa attgactaca taatccatgt gcaatgcttc
    25681 tgctttatct aatctgactt tgtcattgtc caatccagtt accacaacag ttgcgccttt
    25741 actttttaac acttgtgcta caagtaatcc gattggccca ggtcccatta caactgctac
    25801 atcgcctgaa ttgacttgaa tcttagaaac gccatgatat gcacatgcta atggttctgt
    25861 catagctgca gactgatacg atacttcgtc tggaatatga tgcaaacttt cttcacgtgc
    25921 aatgacataa ttagtaaatg cgccatcaac ttgtgttcca ataccttttc gatggttgca
    25981 taaattatag tcttttgatt tacagtattc acactcatta caaacataga atgtcgtttc
    26041 agatgtgaca cggtcaccaa ctttaaaatc tttaacgtct gctccaactt caacgatttc
    26101 accagaaaat tcatgaccta atgtcactgg aaaattaact ttataatgac cttcataagt
    26161 atgaatatct gtgccacaaa ttcctgcata atgtacttta atctttactt tatcatctag
    26221 cggtgttgca acttctttat caagaagttc taagttgcca tgtccttctc ttgtttttac
    26281 taaagctttc accacaaaca cctcgatttt taattgaata gactaaatag tttaaagata
    26341 agatagttaa cgatattacc accttgatca atacttgaaa tttcagatga accttttggc
    26401 atttgtacat tcgtaccttt cgccatatct gtgaaaatgg gtgctacgtc tgttgcaata
    26461 tatagtgaaa ttgcaatcat aatcgtaccc acaatgacag aatgaataat gtttcctctt
    26521 gctgcaccaa caataaacgc gacaacaaat ggtatcgttg ctaagtcacc aaaaggtagt
    26581 acttggtttc ctggtaaaat aacggctaat aaaacagtga taggtactaa aattaatgct
    26641 gtcgaaataa ctgctggatg acctaatgct acagccgcat ccaatccaat ataaatttca
    26701 cgttcgccaa aacgtttatt tagccatgtt cttgcagact ctgaaactgg cattaaacct
    26761 tccattaaga tttttaccat tctaggcatt aataccatta ctgcagccat tgacattcct
    26821 aaattaatga tgtctccagg tttgtaacct gctaacacac caatacctaa acctaaaatc
    26881 aagccgacaa atatagactc tccaaatgcg ccaaaacgtt tttgaattgt ttcaggatca
    26941 gcatctaact tattcagacc gggtactttt tgtaacaatt taactaagta aatacctggt
    27001 gcataagaaa ttgtacttcc tgtagcaatc gatacacctg gtaaatcaaa gaactcactc
    27061 atcatcggtg ctgtccaatc tgctactttc aaacagataa tttggaaaat aactgctgct
    27121 aataacgctt gccaaatact gcctgatacg gcataaacca ttgctgctgt aaacgtataa
    27181 tgccaaaaat tccaaatatc tacattcatc gtctttgtca ctttagttac tagcaataca
    27241 acgttaacta tgattccgag tggaataata aatgctgcga cagatgatgc ccaagcgata
    27301 gatgatgttg ctggccaacc tacatcaatc acattcagac tgacgcctaa atttttaacc
    27361 atcgcttgtg ctgctggccc taaattttta actaataaat cgatgactaa gaaaatccct
    27421 acaaaagcca cacctattgt taaaccagac ctaaatgccg ctccaatttt ctgcctaaag
    27481 aataggccaa gcaagaatat gacaaccggt aaaataacag ttgcacctaa atctaaaaat
    27541 ccccttacaa aatcagtgaa gtaactcata tttaaaccct ccctgttata tatgcattgt
    27601 cacgatactt tccgattgtg attacatttg acgttacagt catttcaacg acaacccttg
    27661 ctaaattcga ctgcagtcct tttgaattac agtcactgcg tttctatgtc atcaacaatc
    27721 atttgtcgtg atagtcattt atatgcaatt tgcatatatt aatatgttat cgacccacgt
    27781 tacatatcaa ttccgttatt tttgtaactc tgttaagatt tgttgttttg tttcttcaat
    27841 accaatacca gttaagaaat tacgtgcgtt gataactggg aatttatatt ctttttttgt
    27901 cattgcagtt gtaactaata aatctgcagt gtcttcataa ggtccaactt ctgtaatttt
    27961 gatttgttta atatctactt taatattgtg ttcctttgcc atttcttcaa ttgcattatt
    28021 tactactgtt gacgttgcaa tacctgcacc acacgctact aatacttgtt tcattttcaa
    28081 ttcctccaat taatttttag ttatattcca aataatcatt gattagtgtt gctaaaattg
    28141 tttcatcttt cgttcgtaga atctgctcca atttttcttc actttgaaaa atttgcatca
    28201 actgttgtaa cagcttaagt tgatcatcta ctttatccat tgctaacata aaaacgattt
    28261 tcacttctgt ctgttgatca agtgttccca tttcaataaa cggcacttct ttttctagaa
    28321 cagccacacc tatcgttcta tggttaatat gttcgacatc tgtatgcggt atagcgaccg
    28381 aacatagatg cgttggtaaa ccagtagcaa attctttttc tctgtcgatg actgcatctt
    28441 taaacgttga cttcacgaac ccattttgaa ataacacatc tgacatttgt gacaatacgg
    28501 attctttatc agttgccgac aaattgagca ttatattttc tttatgcact aattgctgtc
    28561 ccatccattt tccctcgctt ctttatttga ataatttttt aaaatctcat ttacatcaga
    28621 atttttgcga ctttgtatga tgcgcttaat tgcgtcattg tcttgcgcca catctctcaa
    28681 ttgtagtaac gctcttaagt gtgtcacttt atcaacagca gcaataggta caataatatg
    28741 gattgctgtg ccatctgaca tgtatattgg ttcttgtaat atcaacatac tcatcgctgt
    28801 tttatgtaca tgcttttcag agtatgcatg tggaatagca atattttgca tgataaccat
    28861 atacgaatca tcaaatacct gttgcatctc atgaatatat ttaatatcaa taaaatgatt
    28921 agcaactaac acatcacttg ctttagcaat agcttcatca atattttcaa catgatgcat
    28981 tcttttcacg tgccttgccg gtatcaagtc agctaaatct aatgtcttat tttgtgtgac
    29041 aatcgatcca ttaatggttg aaattgaatt ataattggca ataaaatctt ctaaaccatc
    29101 acgtagttct gtaatgtcat taactgtcgt tgtgcgttca attaatgcca ttaacttgtt
    29161 tatttcctta tcaatgtcag ccgattcctt attaatgtac ttcatcactt ctttacgtaa
    29221 ctttcgttgc tcattttcag ataaagctac ttttgtgata aataattttt tatgtgttag
    29281 gacaaacatt ggtgaaaaga cgatgtcata atctaatgtg taattttcaa atgttctaag
    29341 tgaaatcgca tctaagaaaa taatttctgg aaataagttt cgcaactcgt ataacatcat
    29401 ttgtgatact gacgtgcctt gtgtacacac gataatagct tttatcttgc catcgaagtt
    29461 ttcatcttga cgtctcaaac tacctccgaa caacatggtt aaatatgcta tttcattatc
    29521 aggcaacgat tttccgaaat attcagttaa cgattgacat gattgtttca ccatatgaaa
    29581 taaggattga taatttcctt gtaaaggatt tattaattca tcacgatccg ttaagttata
    29641 tttaatccta taaaaagcag gcgttaaatg taacaagagt tgctgtgata atttctcctt
    29701 atcttcaatg ttaataaaag tgatttgttc aaaatggtga atcatttgag cgatggccat
    29761 cgttaaattc gatatgctat ctgattcttg caaatcagtc cattgcacac ttgttgaaag
    29821 taagtgtaat gtcaaatata acttttccgc ttctggcaaa tccggctcat gttgcgtcat
    29881 aatctccgtt gcttgatatt ctttcgtatc cctcaaatac tgataattaa tatttaatgg
    29941 attcatcaca tgaccacttt gaattcgtct acgaatcaca caaaggacat aaggcaatga
    30001 actaagtgat ttgtctataa agcgactctt caaaaattgt tctacctgtt tgatcttgtc
    30061 tttttgatat gcgatatctt cgaatgttaa gttgagcgcc tttaaaactt cacttttagt
    30121 aatatcatga ttcaaccttt gatcaatcaa cttaatgaag aaacggcgaa cttcaaattc
    30181 atcaccaaca atttcataac catgttttcg agaatactta agtgacaaac catgattttc
    30241 caattgctct ttcacatgat ttatatcgtg aatgacagta tttttactga cttgtaaatc
    30301 aattgaaaaa tggtttagag acattgcgtt ttccttacta aaaagcatga gcattaaata
    30361 ataacgacgt gtttctatgc taaaaatgac attgttgccg tttaacattt gctgctccga
    30421 tacatctcgc ttgaataacg tcatgatttc agaacttaca ataaaatttc cttggcttgt
    30481 tctttcaagt tttggataac cctcttgttc aagccacaaa ttgatttttt gaatgcgata
    30541 tcctagttgt ctacgagaca aaccaaatat cgattcaagt tctttaccat gaatagtagg
    30601 attcaataca atttctctga gtatttgctg attatccccg ttcatgttcg attctccttt
    30661 gagcatctga gctcaattta tcatcaatcg tcacaccgat gtacacactt tgaacacata
    30721 ttttcaaaat gagcatgtac atcattgtga tgttttaaca acatttcaat tatatctata
    30781 ttttttgtga ttttaatttt ttaaaataaa gcaattgaaa tttttgcata tatttttgtg
    30841 ttttgtgttt ttttgaagca tttttaacat acatatctca atcattatca aattgtcatg
    30901 accattgtaa cccaatacaa aaaccctaag gacgcttata tcaggcgcct tagggttaac
    30961 tgtatctatt taattaagta ttattattcg tatgtacgta acttatggtc tatcaagttc
    31021 cacacttctt caacatcaac tgctgtagca aaataagcat tggcaggctt acctgtaaca
    31081 tgatttaaat cgacagccat agtgccataa gttagtggac tttgatgttc aatgtcgata
    31141 ttaacgggta ccattgtaaa caattctggt tgtaacaaat acaaaattgt acaagcatca
    31201 tgtattggac caccatccat attaaagtga gtcttgtatg tcttcttaaa gaattgcaat
    31261 aattctacga cgaactgtgc aacaggatta ttgatacttt caaagcgttc aatcacgtga
    31321 tcgtcggcta aaacttgatg tgttacatct aaaccaaaca catttatagt aatcccactt
    31381 tcaaaaacac gcttcgctgc ttcagcatct acccaaatat tgaattctgc tgtaggcgtc
    31441 caatttccaa atgtaccacc acccatcaaa gtaatagatt caatatgctc agcgattctt
    31501 ggctcacgaa tcaatgccgt tgctacattc gtaagaggac ctgtcgctac aattgttaca
    31561 ggtgtatcac tcgtcatcac tttgtttata atcacatctg atgctggcat tgcaactgct
    31621 tgacgtgatg gtgtcgacgg tagtttcgga ccatctaatc cagattcccc atgtatttca
    31681 gaagcaaagg cagctggttt aattaacggc ctatccgcac ctttcgctac tgctatatct
    31741 tggcgtccca taatatccaa tacgttcaag gcgtttgtcg tattcttgtc aactgattga
    31801 ttacctgcga ctgttgttac agctaatatc tctagtggac tgtcaattgc ccccgctaaa
    31861 attaatgcta ttgcatcatc gtgtcctgga tcacaatcca taataatctt tcttttcatt
    31921 tatatatcca cctttcttaa gttgttatcg atagcttatg tatatttatt tatgtggtga
    31981 atcatgttta ttttgaaaaa tagttttaac tttctcatat ttttggatac aaacactatt
    32041 tatctatttt atggcttata aatttatccg atatgcctta tcaacctacc tcgctaaaaa
    32101 taggatgtct acatatctat accgactttt gtcaactcat tttcacaaca atataaacag
    32161 caatttatat gattgttaca tgattcaaac aatttttatg aaaaatattt tcatacacag
    32221 aatatatatt gatattaaat ttctcaaaag ctatattgag aataattagg agggatgttg
    32281 atgaaatctt tatttgaaaa agcacagcag ttcggcaagt cctttatgtt acctatcgca
    32341 atcttaccag ctgcaggtct attgttgggt atcggtggtg cattaagtaa tccaaacacc
    32401 gttaaagcat accctatttt agatattacc ttattacaaa atatttttac attaatgtca
    32461 gctgcaggta gtattgtttt ccaaaattta ccggtcatct ttgcaattgg tgtcgcaatc
    32521 ggattatcta gaagcgataa aggtactgca ggtttagctg cgctgctcgg tttcttaatt
    32581 atgaacgcaa ctatgaatgg cttattaact atcacgggca cattggcaaa agatcagctt
    32641 gcacaaaatg gacaaggcat ggtgctcggt atacaaacgg ttgaaaccgg tgtttttggc
    32701 gggattatca caggtattat gaccgcaata cttcacaaca aatatcacaa agtggtatta
    32761 ccaccgtatt taggtttctt tggtggctct agatttgtcc ctattgtcac agcatttgcc
    32821 gcaatctttt taggtgtatt gatgtttttc atttggccaa gcatacaagc cggcatttat
    32881 catgttggtg gatttgtaac gaaaacaggt gccatcggta cttttgttta tggcttcatc
    32941 ttaagattgt taggtccact cggtttacac catatttttt acttaccgtt ttggcagacg
    33001 gcacttggtg gtactttaga agtcaaaggg cacttagttc aaggtacgca gaacatcttc
    33061 tttgctcaac ttggtgatcc agatgtgacg aagtattatt caggtgtgtc acgctttatg
    33121 tcaggccgtt ttattacgat gatgttcggc ttatgtggtg ccgcacttgc aatttatcac
    33181 acagctaaac ctgaacataa aaaagttgtc ggcggtttaa tgttatccgc tgcactcact
    33241 tcatttttaa caggtattac cgaaccttta gagtttagtt tcttgtttgt cgcacctatt
    33301 ctttatgtaa tccatgcctt ctttgatgga ttagcattta tgatggcaga cattttcaac
    33361 attacaattg gtcaaacctt cagtggaggc tttatcgatt tcttactctt tggtgtgcta
    33421 caaggtaata gtaaaacaaa ctacctatac gtcataccta ttggaattgt gtggttctgt
    33481 ttgtattaca tcgttttcag attcttaatt acgaaattta atttcaaaac acctggtcga
    33541 gaagataaag ctgcagcaca acaagttgag gctactgaaa gagcacaaac tattgttgct
    33601 ggtttgggag gcaaagataa cattgaaatc gttgactgtt gtgcaacgag actacgcgtc
    33661 acacttcatc aaaatgacaa agtcgataaa gtattactcg aaagtactgg tgccaaaggt
    33721 gtaatccagc aaggcactgg tgtgcaagta atttatgggc ctcacgttac agttatcaaa
    33781 aatgaaattg aagaattgct cggggattaa gactaaccga aatatcaaca gaactaatgg
    33841 caacgatgta cgaagtaaga agtgacatcg ttgcttttat ttttaatgtt acatttgaag
    33901 cattaagttc atcatgcact gtagtgagcc cgcaaatcgc ctctgctaga caatcatctt
    33961 aatgctatga ttaaagctta agtgccagat ttgaatttaa tttcaacaac gactttcact
    34021 acattaaaaa tagggctact cgacacatat agttgtatca aatagccctt tatacaattt
    34081 tttgggtaag gttttacaat ttttgggatg gtatagattt tataaaaagt tatttaagtt
    34141 cttctgcttc agccataata tcttttaatg ttttagctga atgtgcgaac ttgctttgtt
    34201 cttcgtcgtt taatgggatt tctacgacgt tgcgaatacc gtttctattg atgactgctg
    34261 gaacaccaat ataaacatct tcttcctcat attcgccttc taataatgct gatacagtca
    34321 atacggcatc ttcatttctg aaaatcgctt cagtaattct agctaatccc attgcaacac
    34381 cataataagt ggcaccttta gcttgaataa tgtcatatgc tgcatcacgt gtttgaacaa
    34441 aaatttgttc aatttgcgct ttgccctcag gacgttgttc aagtaatgtc ttcaaaggtt
    34501 gacccgcaat attagcgtgt gaccatactg gtaattcagt gtcaccatgt tcaccaataa
    34561 tttgagcatc gacgctacgt ggcgcaacat cgaacgcttc gcttaacaat aatctaaagc
    34621 gtgcagagtc taaaattgta ccagaaccta taacacgttc tttaggtaaa ccagagaatt
    34681 tccatgttgc atacgctaaa atatcaacag gatttgtagc taccaagaaa ataccatcaa
    34741 attttgatgc cattacttca ccaacaattg atttgaatat tttcaagttt ttagatacta
    34801 aatctaaacg tgtttctcca ggtttttgtg cagcaccagc acagatgaca actagatccg
    34861 catcatgaca atcactgtat tcgccagctt tcacacgaac tgttgttgga gaatatggtg
    34921 tggcatgttt taaatccata acatctcctc gaactttttc agtgtctaaa tcaatgatga
    34981 ctaattcatc aacaatgctt tggttcacta atgaaaatgc gtagcttgaa cctactgcac
    35041 cattacctat taatacaact ttgttccctt taaatttgtt cattacaaaa actcccttat
    35101 gattaattca ctaacataca tgtagcttca aatatgttag tttaatgctg cttattgacg
    35161 atacaaaagc aaataaacat ctcttttatt ttcaacgcat aacttaaaag gtcatgtgtc
    35221 atccgctttt aagtttgtga tttatttcac atataaaatg taacatgcat taagtactgg
    35281 gtcaatatta aattgtgatt tatttcacat tttattttaa tttttacacc tttttaattt
    35341 gtatacgatt acatcttaga tgtctttagt cttcgtactt cgccagtgat tatttacact
    35401 ttcacatttt tattatcatg tttacttttt tctaggaaaa caacaatgtt ttttgaatta
    35461 gtcaaataaa tgcgctcaat cgtcggtgtg caaacagaca attgtacaca atgcttattg
    35521 ataagtattt aaaaaattaa aaatgtcata caattatcaa atttgccatt ttatttatat
    35581 tttctcaaac caattaattg aatatcgaaa tttttagtag aataatcaaa atatacagat
    35641 taaaggagga gtatcatgct tacagaacaa gagaaagaca ttatcaaaca aacggtgcct
    35701 ttacttaaag agaaagggac agaaattacg tcaatctttt atccaaaaat gtttaaagcg
    35761 catcctgaac ttttaaacat gtttaatcaa acgaaccaaa aacgaggcat gcaatcttca
    35821 gcattagcac aagctgtaat ggccgcagcg gttaatatcg ataacttaag tgttattaaa
    35881 ccagtcatta tgccagtcgc atataaacac tgcgcactac aagtttatgc tgaacattat
    35941 ccaattgtgg ggaaaaattt attaaaagcc attcaagacg tgacaggatt agaagaaaat
    36001 gaccctgtca ttcaagcttg ggcaaaagca tatggcgtta ttgcggatgt gttcatccaa
    36061 attgaaaaag aaatttatga tcaaatgatg tggattggtt ttaaaccatt caaaattacc
    36121 aatattaaac aagaatctga agacattaaa tcatttacag ttgaaactga agaatatgac
    36181 tttagtgaat ttacaccagg ccaatacatc acagttgatg tttctagtga taaacttcca
    36241 tatagagcta aacgtcacta ttctatcgta tcaggtgaaa aaaaccattt aacttttggc
    36301 gttaaacgtg atgtcacaac agaacatgaa ggcgaagttt caacaatttt acacgatgag
    36361 attaaagaag gcgatatgat taatttagct gcgcctgtag gtggattcgt attagagaat
    36421 acgactgaac cacaactttt cttaggttca ggtattggtg ttacaccttt agtagctatg
    36481 tatgaagctg cctctgccaa aggtttagat acacagatgg ttcaagttgc tgaaaatgaa
    36541 caacatttac ctttcaaaga caacttcaac agtatcgcaa gccatcatga caacgctaaa
    36601 ttatatacac acttaaaaga taaacaaggc tatattggtg ctgaagaatt acaagtattt
    36661 ttagcaaata aacctgaaat ttatatctgt ggtggtacaa aattcttaca atctatgatc
    36721 gaagcactta aatctttaaa ttacgatatg gatcgcgtac actacgaaac atttattcca
    36781 agactaagcg ttgcagtata actgagaaaa gaggtttagt gctcatatga aacttaatca
    36841 acgttacgta aaagtatttg cattatattt cgtaagtatt gttactgcaa atattattgt
    36901 taaaaataat aatttaatta aaacattgat acaaaccata gccgggtaca cggtctttgc
    36961 agttggtttg aagtatttaa ctaaacgtaa aaataaatga catctatacc aaaacagcta
    37021 tgactttact ttgtcatggc tgttttttaa ttgggagtag gacagagatg atattttcgc
    37081 aaaatttatt tggtcgtccc accacaacat gcattgatgt atgctcactg aatttcataa
    37141 gaaaggagtt cacaagatga ccgtagatat tggacggatt tatgacaata aagataatac
    37201 cgacgctatt cgtatcctag tcgatagagt ctggccgaga ggtatttcga aaagaactgc
    37261 taacctagat tattggttaa aagacattgc cccttctact gagttgcgac aatggttcca
    37321 acatgatcct aaactttttg gagcttttaa agaaaaatat gaaaaagaat tacgtgatca
    37381 ggatgcgcaa aaagatgctt ttgaaaaatt aaaggatatt gtaaatcagc ataatcatgt
    37441 tctattgtta tatgcagcaa aagatactaa acataaccaa gctgtagtac tacagcagtt
    37501 gctcaatact tagttatata tgtttatcgt gaatcactat aaatgtcgct aacttcattt
    37561 ctaatattta tttttaaaaa gcataattac tacaattaat tgaactttaa taattactaa
    37621 acttgaacaa cattttactt taacaaaata aagttttaaa atattaattg ttgggtttta
    37681 catagtaatt agaaagagag tgttaatgaa atgaagttta aaagactagc aactatattt
    37741 tcagcagtat tagtgttatc aggatgtggc tcaatgcatt catctggcaa agacttaaat
    37801 atttcgttac cgttaaaaac aaagtctatt gcaccttatg aaacagatgt accagttaag
    37861 attggtgctg cagaatcatt atttaaaaca aatgatcaag gaaaaataga aaaagcatta
    37921 gtgaaatcgt atcatcaacc aaatgataca acgttagata tagaattaaa agataatatt
    37981 aaatttcaaa acggtcaaaa attgactgca gaaaaagtga aatctagcct tgaaaatagc
    38041 atgaaaaaaa gcgacttggt caaatattca ttaccaatat catcaattac cgctaaaggt
    38101 caaaaactga caattaaaac caactccgct taccctgaac ttgtatctga attagctaat
    38161 ccttttatgg caatttatga tacagatgct aaatcagatg ttaatcaaac tcctgttggt
    38221 acaggccctt accaaataaa agattataag caatctcgaa aaatatcatt gtcgaatttt
    38281 aaggactatt ggcaaggtaa accgaaactt gatcatatta ctgtgacgta ccaagaagac
    38341 ggcaataatc gcgtcagaaa tttagaatct caaaaagatg atttaataac tgatgtccca
    38401 gttaataaag ttcaagacat agaaaataat caaaatttaa aagtgtcaaa agaatctgga
    38461 tttagaactt ctttacttat gtataatcat actaataaaa aaatgactaa atccgttcgt
    38521 gaagcattag atcatatcat tgatagacaa ggtattgcag atcatattta tcaaggttat
    38581 gcgaaacctg caacgagtcc atttaatgat aaaattccat atattaaaga acctaagtta
    38641 accaaacaaa atatcgaaca agcaaaaatg ttattagcta aagatggtta tacaaaagaa
    38701 caccctttaa aaattaagtt aattacgtat gacggtcgtc cagagctatc gaaaattgcg
    38761 caagtattac aatcagatgc taaaaaagca aatattgaaa tcgacattaa aagtgttgat
    38821 gacatagaag gttacttaaa agaccgttct gcatgggatg caacgatgta tagtttcgga
    38881 acaattcctc gtggagatac aggttatttc tttaatcaag catataaaaa agatggtgct
    38941 attaataaag gagactacaa taatagcaac gtcgatgatt taattaatca attgaatcat
    39001 actgtagatg ttaaggaacg tcacaatatt tctaatgata ttattaaatt atcaagtcga
    39061 gatgtaccaa acagttatat tgcttacaac gatcaaatag tcgctgctaa ctctaaggtt
    39121 aaaaattata aagtaacacc tgaaggtatc tatttaatcg attatcgaac aacgattgaa
    39181 cgataaggga gtgtgggaca ttgatttatg tctcagccct gcttcaacta taaaaaccac
    39241 gccgaagtca tccaatatga ttatggcgtg gtttaatttg cttatatgat tgtattaatt
    39301 tctgtttgat gaatttcaat aaaatgatta ttacatactt tcagtgaatc atgttcattt
    39361 tatataaatc taattgtcac tatttgttta acgtgaaaca cttttttaac tccaaaaagt
    39421 attcctattc cactctttta aagacaaatt ataaaaatag cttatgtcac aaacatatga
    39481 gcaacataag ctttaattat tattcgtcta cttttttctg ttgttcttta aattctttct
    39541 gcagtaaatt cacttgtttt aaacctgccc ccattaaaat gaatacaagt atccacacaa
    39601 taacgattaa aataaatatt ttcaacgtat cactgccaag tacagttaaa gatgtatccc
    39661 aaatgccatg taaaacaacg gctgataaaa agaatattaa aaagcgttta tcaaaaatat
    39721 ctttgaattc aaagccatgc tgttctttcg caataactat tgcagcacca acaatcgctg
    39781 accaaactaa atgaccacca atcgcagtcc acgcacgtgt gaagacaata tctaataatg
    39841 ggacattttc tcctaaagcg aaattcaaaa tataacctgc tgattcaaaa actgcgaacc
    39901 ctgcaccaat agcagcaccg attaataatc cattcaaaat cttatttgtt ttcaatttat
    39961 tgacgaaata aacaataatg agtgcttttc cagtttcttc aactaatcct actaaaaatg
    40021 catcgaaaaa tgttaaagaa ccaaaccttt cgaattgatc actaaaaacg acaaatctat
    40081 ataataccat cgtacttagt aatgaaaata cgccgccaat aaagaacatg ataataactt
    40141 caaaaatgct aatattttta aacgcatttg attcataaaa gaagaacaaa cccgataatg
    40201 gtactgttaa agcccctata aaaatgagac ccggaatcgc gttagtgtta ttaaaaattg
    40261 ctgccatgac ccataatcca ataaatgtta ctgtgaaagc gatgaatact cgagaaaaga
    40321 gccatggctt cccccattct tctgatatgt cacaaattgc tggcgtagtt ttagcagtac
    40381 ccgctataaa tatttcatct gcttcgtttt tagtatgcgg cttaaatact tcagaaagca
    40441 tatcttttaa attaagattt aagctttcat cattgcccgc tagtctaccg attgatttgg
    40501 ttgcattatc aaataaactt cttgcttcgt tttttaaatc tgtattttgc gctgtttcat
    40561 gcgctttagc ttccatttgt ttatcgatag attcattatg tatgtcggac gtcttagttg
    40621 attgtgtact tgctgatgac atcccctctt cacctgtatt aggtgcaact gattgcgttt
    40681 gtgttgtctc ttctttttct gtatttcttc gatgattaag accttccata gacttaccta
    40741 ctttcacttt tcataataaa actattttaa tttaatattg atggattttc catattttaa
    40801 tataaatact ttgttcgatt gggcactttg tatttcatat ttaaaaatca aaacctattt
    40861 aactttttat attccgaaca atatttttat ataagtattt taatgatatt caattcatta
    40921 caaacccttg atattacaat tgttatcgac cgtcaaaatg tgtctatact gtgtctatta
    40981 tcagaataaa aagatatttc taattgattt taaacgtcgt tatgttatat ttcttgtaag
    41041 ggtttacaca aagtgtaaaa acgctatatt ttatcaaatg tgatggaggt gacttgcttg
    41101 aaacaaatca catggcacga cttacaacat atcattaaag atggtgatgt gattggttta
    41161 ccagcattag ctgtagccaa cttacccgcc gaagttctac gtgctgtgtt agcgcaacat
    41221 gacacatatc atacgcccaa tgatttaacg tttatattag cgaatgatat tcatagttta
    41281 ggtgccgcac cggatttaga tgattttata gaacgtcgca tgattaaacg tgtcattatg
    41341 agtattttaa cggcttcttc caaaacggca caagcaatga aaaataatga cattgaagct
    41401 tattttttac cacaaggtat cattgcaact cattatcgtc agagtaatca attattacct
    41461 ggagttatta ctaaaatcgg attaaacaca gctgttgatc ctagatacgg tggcggtaaa
    41521 gtaaatacac gaacaacgga tgatttagtt tcattagtaa ccatcaacga tgaaacatac
    41581 ttacattaca cattccctag cgttgatgtg gcactactga gaggaacata cgcagatcaa
    41641 caaggtaaca tttatttaac tcaagaagcg tacttgagcg agtgttatca tgtcgcatta
    41701 aacgcgaaag ccaatcatgg gaaagttatt gtacaagtta aagctttagt tgacgactat
    41761 caactaaaac cgaatgaagt tgttatccca ggaaatcttg tcgattatgt atacgtcaca
    41821 gaagatgaaa agaatcaccg ccaagtaatt caaagtcatt atttaccagc cttgtctgga
    41881 gaagaacgaa ttgatggaat acctgaaccc gcattacctt ttaatagtcg caaattgatt
    41941 ctccgacgtg ctgctcagtt tttaacttat ggcgatacaa ttagcatcgg ttatggcatc
    42001 aataatgaac tctctaattt attgcatgaa gaatgtgttg aacatgatgt gcaaccgatt
    42061 ttagatgttg gcattttcgg tggattcgtt gggagtcgtg aacattttgg tatgaattac
    42121 aatgcagatg tgcgcatgcc tcatgatcga gcatgggatt ttatttataa caatggtgta
    42181 tcagttgcct atcttagctt tgctgaggtt gatcaatacg gcaatgtcaa cgtgtcttac
    42241 ttcaatgacc gactaaatgg atgtggtggc tttatagaca ttacgcaatc tgtaaataaa
    42301 attatctttt caggtacttt tgtagctggc agtcatgtct catgccataa tcaacgatta
    42361 aacattgaaa ctgaaggaca aaaccagaaa tttgtatcag atgtgagcca tatcgacttt
    42421 aatgcacaat attcacaatc actcgagcaa gaaatctatt ttgttactga tcgtgcagta
    42481 ttcgaactca ccaatcaagg cttgaaacta attgaaattg caccaggtct tgatttgcat
    42541 aaagatattt tgaatcaaat ggcttttaaa ccaattattg ctgatcattt aaaattaatt
    42601 gataccagca tttacaaaga aaaatgggga caacttaaac aatcaattca taaagtatga
    42661 aagtgaagct aaagggggta tactaatgaa tttcgattgg ataaaaacgc gttcagactt
    42721 cgatgatgac aagcctgccg ttattgatca cgcaaaacaa acatcttgga cataccaaca
    42781 actcaatgca cgcgctgata atatggcaca ttatttaaca tctcaaggtg ttaaaaaagg
    42841 cgatgttatc ggtatttttg cgccaaatga tattgcaata ttagatttat tgtttgcttg
    42901 ttttaaaaca ggtgcggttt ttttaccatt gaattggcgg cttaacccaa aagaaattgc
    42961 agccattgtg gaagatgcac aattaaaact gctcttctat gctgaaaaac atttaagttc
    43021 actcaccgat attgaccaaa acttattgca tatggatatt gatgtggcgc aatatgatga
    43081 aatcgtaaat ccagattatc accaaccttt tcaagcaaca cctgttgaac cacaagatct
    43141 tgcagcatta atttatacaa gtggtacaac cggatcacct aaaggtgtga tgttctcata
    43201 tgaatcattt gttcataacg gtgcaaactt agagctgacg tataagttca attcaaacta
    43261 tattacgatt gtatcaacac caatgttcca tgttttaggg tttaacgata ctgtattacc
    43321 agtattaatg tcaggtggca cacttattct tcaacgttac tttaacggcg aagaattgaa
    43381 tgacatgatt gcacaatatc accctacatt tattatcatg attccgacaa tgtattatag
    43441 tacgctacgt gctagtaatt ttaatcctga aaattttaga gctatggatt atatcatcca
    43501 aggtggttca caaccattac caagtatcca agcagctttt aaacaatatg gcataaacat
    43561 tattaacggc tatggtttaa ctgaagcacc tcttgtactt gttaatacac cggaaaattc
    43621 aaaacgtaag ccaatgagta ttggtaaagc agtcatgttc gttgatgcac gtatccttga
    43681 tgataacggt gaggaagtac ctactggtga gattggcgaa cttgcaatta aggctaaaaa
    43741 tgtcacgcca ggatattgga ataaaccagc agagactgcc aaagcatttc atggtcgata
    43801 tttattaact ggtgacttag cgaagatgga caacgatggc gatatattta ttattgaccg
    43861 caaaaaagaa ttaatcataa ctggtggcga aaatgtctta ccatccgaag tcgaaaatgc
    43921 tttagctgag catccactag tagaccggtg tgtggtcgtt ggctatgatc atccaaaata
    43981 tggtgaatca attgctgcag ccattatact tcgcgaagat gaacctcatt acgctgaaat
    44041 tttaaatcaa catatgcgaa gtcgtttagc aggttataaa gtcccaagaa tgtatgtacc
    44101 agtgacacat atgccgttaa acagtacgca gaaaccagat aaacttgcga ttcgacaaat
    44161 gatgaatgac aaagtctcgc aaacacttta aaggtgataa aaatttttga catttagtgt
    44221 aagcgtttac aaataaagcg tgttgttttt gaattaaatg catttcacat tagtattcat
    44281 attattttta ggaggaattt atatgacatt tgaaaaagaa acggtcttaa aaacattatt
    44341 tcctgaagat gtacttagta ttgctaaagg tttaacagac ggtgaagtcg aatttttaca
    44401 acaagtagat tcattgctag aaagtaagta ccgtgaaaat attaatcaac attggataga
    44461 cgctactgta cccgaggact attttaaaga tctgggagaa ttaaattatt ttaacaatcc
    44521 attactttac aaggatcgtc caaacgccaa aatgcctagt caactatttc agtttttcat
    44581 gtcttaccta ctcgcgcgat ttgatatttc cttagctacc ctactcggtg ttcaccaagg
    44641 tttagggcat aacactttct atttcggagg tagcaaagaa caaattgcga aatatgtacc
    44701 taaattacag tcacatgaac tgcgtacatg ctttgcttta actgaaccag aacacggttc
    44761 ggacgttgcg ggaggtcttg aaacagtcgc tgaacgccaa ggcgatactt gggttatcaa
    44821 tggtgaaaag aaatggattg gtggtgcaca tgtatctgat gtcattccag tattcgcagt
    44881 aaataaagaa actggcaaac cccattgctt tgtagtcaga ccagaacaag atggcgtcga
    44941 tattgaagtc attgataata aaatcgcact tcgcattgtt cctaacgccc taattaaatt
    45001 aactaatgtc aaagtagatg aagcggatcg cttacaaaac ataacaagct ttaaagatat
    45061 tgccaaaatt ctttattcaa cgagagcagg cgttgcttat atggctacag gtggtatggc
    45121 tggcgcttta cgtgccacat tagattatgt cactgagcgt aagcaattcg gcaaaccaat
    45181 tagtaaatat cagttaatac aagaaaagct agcaatgatg caaggtaatt tagctcaagc
    45241 aatggcaaca tgtgctcaat tagctaatat gcaagcacat ggtgaatatg acgaggttgc
    45301 aacttcaacg gcgaagatga tgaatgcctt acgtttgcgt gagacagtag ctatgggccg
    45361 cggtattaca ggtggtaatg gcatactagc tgacgattat gatattgcac gtttcttctc
    45421 tgatgcagaa gcgatttaca cgtacgaagg tacacatgaa attaatgcct tagtaattgg
    45481 acgcgctttg actggagatt ctgctttcgt ataaatagca aataattata tgagatgcat
    45541 taatttcact aaaaaagact tattttaagc ataaagcttt ttccttaaat aagaggctaa
    45601 gatgactgtc aaagatactt aattaatttt ataaaatagc aacgttattc caattatctt
    45661 aatggttatc ttatcctcaa ctaaattgga ggaatcacta tgacaattaa taaagtaacc
    45721 gttcttggcg caggcacaat gggcgctcaa ctggcagcac tttttgtgaa tgctggactt
    45781 aaagtaaaac tattagatat tgtagtggac aaaaacgatc caaatctcat tgcgaaaaaa
    45841 tcttacgata aaattacaga taagaaacgg ccgctactat tcgacttaaa tctagcgagt
    45901 catttaacat atggtaattt tgatgatgac ttggtaaatg atgatgctga tttatatatc
    45961 gaagcagtca aagaagatat tgaaattaag catgctgttt ggcaacaagt tctacaacat
    46021 gctaaagaag atgctttatt cgctacaaat acatcaggta ttccaattaa tgcgattgct
    46081 caagcattta acgagaagga tcaagaacga ttctttggtc tacatttctt taacccacca
    46141 cgtattatga aattagtgga gttaatacct acgtcacaca cgaaggaatc tattatatta
    46201 gatgtaaaaa atttcgcgca aaatgtgtta ggtaaaggtg tcattgtcgt caatgatgtg
    46261 cctggctttg tcgcaaatag agtcggcacg caaacaatga atgatattat gtatcgcgcc
    46321 gagcaacaca agataagcat tgtagatgtg gatgctttaa ctgggcaagc gattggtcgt
    46381 cctaaaacag gtacatatgc gctatctgac ctagtcggtt tagatattgc agtgtctgta
    46441 attaaaggca tgcaacaagt acctgaagaa acaccttatt ttcatgatgt caaaattgta
    46501 aatacgttgt ttgacaatgg cgcactcgga cgtaaaacga aacaaggatt ttacaaaaag
    46561 gataaagaaa ctaaagctcg acttgtttac gatgttgaaa aacaagatta tgtacctgta
    46621 tcgcaaccac aattaccaat tttaaatgaa tttaataaag acttagtgca taaccttgat
    46681 accatattca atgcgcaaga cgaagcggga ctatttttat gggagacatt acgtaataat
    46741 ttctattact ctgctatcaa tgtacctaaa gctaccgatg atttccgaga catagaccgt
    46801 gcgcttgtct gggggttcaa ctggaaactt ggtccattcc aattatggga tgcaatggga
    46861 tacgaacgtg ttaaaacacg tatggaagac gaacttggag acttaccaca atggattagt
    46921 gatttagatg gtggctttta taaacaagat gagaccattg aatatgcaac acctatttct
    46981 cacttcgtaa aagatgaact ttgggataaa ggtgatgcca aactttccgt aactcatgat
    47041 gatcaactgt tactgaaatt acaaagtaaa aataatgtca ttaccgatga attcaacgat
    47101 gcgttagttg atgcgattga tttactggaa aatgaccatt acacaagtat ggttatttat
    47161 gcagatggta acaatttcag tgtgggtgct aaccttttct taatgaaaaa ggcgcatgaa
    47221 gacggtcttg tagatgatgt cgttgcacaa tcaattgata aattacatta tagctttaat
    47281 cgtttgaagt atagtttgaa accagtagtc acagctgttc aaggtcgtgc cttaggcggt
    47341 ggctgtgagc ttgtacttta ctcacctatt gttgtcgctg caagtgaaac atatatcggt
    47401 cttgttgaag caggtgttgg cttattaccg agtggcggtg gccttgcaga aatggctgat
    47461 cgcatattac gcacatcgca taagtttgat gacaaacaag cttccatgac aaaagtactg
    47521 acgaatatcg catttgcgaa agtctctaca aatgcctttg aggcacgtcg ttatggttat
    47581 ttacgtgata cagatacgat tattttcaat acagcacaac gtgtcgaagt tgcgctcaaa
    47641 cgtgcgaaat atgaagcaga aacaaactat attccgaatc ctagacatca atatatcgct
    47701 ttaggtgaag acttcaaagc attgatccaa ggacaattag atgcgcaaag acggggtcat
    47761 tttattagcg accatgatta tcatattgcc ttaaatatcg ccacaatttt agcgggtggt
    47821 gatttaccaa gaaatacatt tatcaatcaa cgttacattc aatcgttgga gaaaattggc
    47881 tttattgact tactaaaatc taaaaaatca tatgaaagaa ttgcacatat gttaaaaact
    47941 ggtaagccat tacgtaatta aaagatagtc attaagagag gatgataacc atgcaagaag
    48001 catacattgt agcttatggg cgttcagccg cagcgaaagc aaagcaaggc gcattattcc
    48061 acgaaagacc tgatgatgtc gcagccaaag tattacaagg cgtattgaaa cgtattgacg
    48121 gaaaattcaa taagaatatg attgaagatg tcattgttgg tacggctttt ccagaaggat
    48181 tacaaggcca aaacattgca cgaacgattg cattgcgtgc gggattatct gacacggtac
    48241 cgggtcaaac agtgaatcgc tactgctcat caggattaca aaccatcgcg attgcagcca
    48301 atcaaattat ggctggtcaa ggagatatac ttgtagctgg tggcgttgaa ttgatgagtg
    48361 ccgtaccaat gggtggcaac gagcccacaa acaatccaac cttacaatat gatgatatag
    48421 gtgcgtcata tcctatgggt ttaactgctg aaaatgtagc atcccaattt gacgtatcac
    48481 gtgaagatca agatgcttat gctgtcagaa gtcatcaacg tgcctatgac gcacaacgtg
    48541 atggtcggtt caaagatgaa attattccaa tacaagtaaa ctcagttgaa tatacaaacg
    48601 caggaccaaa agtacacaca aatatctttg accaagatga atttatacgc cctgacacca
    48661 cgatggaggc attagccaaa ttacgtacag tatttaaagc tgacggcact gtgactgcag
    48721 gaacatctgc cccactttct gatggtgcag gatttgtagt tttaatgtct ggagataaag
    48781 tgaaagaact cggcgtgaca cctattgcac gattcgttgg ttttaaggca gtaggcgttg
    48841 acccgaaaat tatgggtatt gggcctgcat atgcgattcc tgaagtattg tcactcagca
    48901 atctatctgt tgaagacatt gatttgatcg aattgaacga agcatttgct tctcaaacga
    48961 ttgcatctat taaagaagta ggtctagata tatcacgtac gaatgtgaat ggtggcgcta
    49021 ttgctttagg tcatccatta ggtgctacag gcgcaatgtt aaccgcgcgt ttacttaatg
    49081 aaatgggtag acgtcccgat agccgttacg gcatggttac gatgtgtatt ggtgtcggca
    49141 tgggtgcagc tgctatattt gaatatgtgc gttagaatgg ttgattttgg atgaagcgga
    49201 ttcgttttgt tattgaatga agtaggctga agttgaagcc agttgaagtt gaagcgggtt
    49261 gaagcaattt cgttttatta atgaagctgt gtgaaatata gtgattgaac aaaaaaagtg
    49321 gtttaatggg atggtggtta tttccgtttt agaatttaac atttacacgt ctaattttaa
    49381 tcattgtttt aaattttatg aatcgaagcc ctttgattta ataatatttg ctaatgctag
    49441 taacttatct gattgttcat gtttaaaata aagaaaacca ctcacatcag tgtgtgttcg
    49501 aactagactt gtaagttcca gttcggcacg actttctaaa gcaattatta ttgctgtgat
    49561 tgtcgtatat cacttagatg tgcgtggttt attttaatag gttagtaata tattaggtca
    49621 tgttatgttt aagactataa tgaataaata atttagaaat atgcttccga ttgttcgatg
    49681 ctttaattca gttagaagca tcatagaatg catgattact gttgtaaaga tacgtaatgt
    49741 tttgtattga ctgtatgtct ttggatagag ttacaaactt attttgttac tctaggccca
    49801 tatgtcgcag taccatctgc atgtgttgtt acattgtatg catttgtttt acttggcttc
    49861 ttgtatgtcg ggcgagctcc gtatgacact tgaccgtttg catgtgttgt tacgttgtat
    49921 gcatttgttt tgcttggctt gttttgtgtt gggcgagcgc catatgatac ttggccgttt
    49981 ccatgtgttg ttacgttata tgcgtttgtt ttgcttggct tgttttgtgt cggacgagct
    50041 ccgtatgata cttgaccatt tgcatgtgtt gttacgttat atgcatttgt ttctgatggc
    50101 ttattgaatc ttggtctcgc ttcatatcca aatgttccat cgttgtattc acggatacct
    50161 gtaccagcat ctctatattt aacatattta ggtgttttgt taaattgcgg tctcggacca
    50221 tattgagaag cttctgttgt ttcagttgct tgaggtttaa cttcaatatc acttgattct
    50281 ccttgagtac cttttaacgt tgattcagta ccttgtggtt ttatttcaag tttagatgag
    50341 ctaccttcaa gaccttctaa aatagggttc gttaacggtg ggtttgtata attattgctt
    50401 aatgatgggc cgctttgttc cattgttaga aaatcgggac cttgaacgat ttcaccttgt
    50461 accgttttat tttccatcgt tggatattcc ggacctttta caatttcacc tgtaattgtg
    50521 ccctgtggaa ttttaactaa tggttgtgca actggttgtg ttgtttcttc agctttacca
    50581 gccgtagttt taacctcttg ttggttatca actttaggtg cttgaggttc ttcaactttc
    50641 ttctcttctt ttactactgg cgattttgtt tcagtttctc cgtatttttt gacagttttc
    50701 tttttccaag aatcatctgc ttctttaact gcttttttcg tttcttcaac taatttatca
    50761 aaattaggtt tattatcact atttgtttta tagttatgtg ttgtaggatt atatttcgtt
    50821 atagatttcg gtctattttg tttagtttcc ataaagaaat catcaataat tgaatttaag
    50881 tcatcaatca tttctttttt aatacgttca tttgtaattt tatgtggatt gtctgtatct
    50941 ccaaggatta agtccagttt tgctcgtaac tctttcgcgt gctccccata atccttatca
    51001 ccataatatg atacaactaa tgtatcaatt tcagatacga gatcgtatac ttccttagtt
    51061 gctttatctt cttctgctgc attaaaagtt ttcaagtctg aattcttatc cttaatatct
    51121 ttaacttctc tgtgaaaatc atccagtgct ctctttaatg catcctgtag ttcattgtat
    51181 tctttcatcg aaagttcttc taaattatat ttatgaaaat tagccatttt taaatctgta
    51241 cgaggatttt cttttttata atttgcatac cattgtttat aatcttcata ttgagatttc
    51301 tttctctcca aaagatattg atcttccctt aatacctttt ccaacaacct atctttagct
    51361 tctttataaa tattatctcc atattcatat
//
LOCUS       DAKTIP010000002        49537 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAKTIP010000002 DAKTIP010000000
VERSION     DAKTIP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 49537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49537
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.002"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            46..570
                     /locus_tag="O0305_000047"
     CDS             46..570
                     /locus_tag="O0305_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCY0273234.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            597..1835
                     /locus_tag="O0305_000048"
     CDS             597..1835
                     /locus_tag="O0305_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCY0273235.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALGMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            1863..2492
                     /gene="upp"
                     /locus_tag="O0305_000049"
     CDS             1863..2492
                     /gene="upp"
                     /locus_tag="O0305_000049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCY0273236.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            2516..3643
                     /gene="wecB"
                     /locus_tag="O0305_000050"
     CDS             2516..3643
                     /gene="wecB"
                     /locus_tag="O0305_000050"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCY0273237.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            3711..4163
                     /locus_tag="O0305_000051"
     CDS             3711..4163
                     /locus_tag="O0305_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCY0273238.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            4184..4912
                     /gene="atpB"
                     /locus_tag="O0305_000052"
     CDS             4184..4912
                     /gene="atpB"
                     /locus_tag="O0305_000052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCY0273239.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            4955..5167
                     /gene="atpE"
                     /locus_tag="O0305_000053"
     CDS             4955..5167
                     /gene="atpE"
                     /locus_tag="O0305_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCY0273240.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            5365..5886
                     /locus_tag="O0305_000054"
     CDS             5365..5886
                     /locus_tag="O0305_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCY0273241.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            5886..6425
                     /locus_tag="O0305_000055"
     CDS             5886..6425
                     /locus_tag="O0305_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCY0273242.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLR
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            6447..7955
                     /locus_tag="O0305_000056"
     CDS             6447..7955
                     /locus_tag="O0305_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCY0273243.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            7986..8852
                     /locus_tag="O0305_000057"
     CDS             7986..8852
                     /locus_tag="O0305_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCY0273244.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            8874..10286
                     /gene="atpD"
                     /locus_tag="O0305_000058"
     CDS             8874..10286
                     /gene="atpD"
                     /locus_tag="O0305_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCY0273245.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            10306..10710
                     /locus_tag="O0305_000059"
     CDS             10306..10710
                     /locus_tag="O0305_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCY0273246.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            11048..11140
                     /locus_tag="O0305_000060"
     CDS             11048..11140
                     /locus_tag="O0305_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273247.1"
                     /translation="MLKSKFPSPLSTVDISFFEIIYIFSYQDIL"
     gene            11272..11505
                     /locus_tag="O0305_000061"
     CDS             11272..11505
                     /locus_tag="O0305_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCY0273248.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            11616..12881
                     /gene="murA"
                     /locus_tag="O0305_000062"
     CDS             11616..12881
                     /gene="murA"
                     /locus_tag="O0305_000062"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0273249.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRDAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            12915..13355
                     /gene="fabZ"
                     /locus_tag="O0305_000063"
     CDS             12915..13355
                     /gene="fabZ"
                     /locus_tag="O0305_000063"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCY0273250.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(13414..13854)
                     /locus_tag="O0305_000064"
     CDS             complement(13414..13854)
                     /locus_tag="O0305_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCY0273251.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            14048..14395
                     /locus_tag="O0305_000065"
     CDS             14048..14395
                     /locus_tag="O0305_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0273252.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSI"
     gene            14832..15527
                     /gene="sceD"
                     /locus_tag="O0305_000066"
     CDS             14832..15527
                     /gene="sceD"
                     /locus_tag="O0305_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCY0273253.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGTNGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKSSEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      15731..15833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15913..16602
                     /gene="tenA"
                     /locus_tag="O0305_000067"
     CDS             15913..16602
                     /gene="tenA"
                     /locus_tag="O0305_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCY0273254.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLSGDIGADALRHYLKAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            16595..17425
                     /gene="thiD"
                     /locus_tag="O0305_000068"
     CDS             16595..17425
                     /gene="thiD"
                     /locus_tag="O0305_000068"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0273255.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            17409..18200
                     /gene="thiM"
                     /locus_tag="O0305_000069"
     CDS             17409..18200
                     /gene="thiM"
                     /locus_tag="O0305_000069"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCY0273256.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            18202..18843
                     /locus_tag="O0305_000070"
     CDS             18202..18843
                     /locus_tag="O0305_000070"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCY0273257.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            18929..19801
                     /gene="yidC"
                     /locus_tag="O0305_000071"
     CDS             18929..19801
                     /gene="yidC"
                     /locus_tag="O0305_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCY0273258.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(20614..21261)
                     /locus_tag="O0305_000072"
     CDS             complement(20614..21261)
                     /locus_tag="O0305_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0273259.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNCHD"
     gene            complement(21289..22773)
                     /gene="cls"
                     /locus_tag="O0305_000073"
     CDS             complement(21289..22773)
                     /gene="cls"
                     /locus_tag="O0305_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0273260.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            22939..23232
                     /gene="csoR"
                     /locus_tag="O0305_000074"
     CDS             22939..23232
                     /gene="csoR"
                     /locus_tag="O0305_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCY0273261.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            23244..23453
                     /locus_tag="O0305_000075"
     CDS             23244..23453
                     /locus_tag="O0305_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCY0273262.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            23581..23718
                     /locus_tag="O0305_000076"
     CDS             23581..23718
                     /locus_tag="O0305_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273263.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(23982..25184)
                     /locus_tag="O0305_000077"
     CDS             complement(23982..25184)
                     /locus_tag="O0305_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCY0273264.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            25502..26572
                     /locus_tag="O0305_000078"
     CDS             25502..26572
                     /locus_tag="O0305_000078"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCY0273265.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            26587..27945
                     /locus_tag="O0305_000079"
     CDS             26587..27945
                     /locus_tag="O0305_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCY0273266.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLIYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLINDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            28462..29982
                     /locus_tag="O0305_000080"
     CDS             28462..29982
                     /locus_tag="O0305_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0273267.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(30351..31046)
                     /locus_tag="O0305_000081"
     CDS             complement(30351..31046)
                     /locus_tag="O0305_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0273268.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(31046..33703)
                     /locus_tag="O0305_000082"
     CDS             complement(31046..33703)
                     /locus_tag="O0305_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCY0273269.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            33841..33951
                     /gene="kdpF"
                     /locus_tag="O0305_000083"
     CDS             33841..33951
                     /gene="kdpF"
                     /locus_tag="O0305_000083"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCY0273270.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            33975..35651
                     /gene="kdpA"
                     /locus_tag="O0305_000084"
     CDS             33975..35651
                     /gene="kdpA"
                     /locus_tag="O0305_000084"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCY0273271.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            35670..37697
                     /gene="kdpB"
                     /locus_tag="O0305_000085"
     CDS             35670..37697
                     /gene="kdpB"
                     /locus_tag="O0305_000085"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCY0273272.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            37717..38277
                     /locus_tag="O0305_000086"
     CDS             37717..38277
                     /locus_tag="O0305_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCY0273273.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHVSTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            38485..38964
                     /locus_tag="O0305_000087"
     CDS             38485..38964
                     /locus_tag="O0305_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273274.1"
                     /translation="MSDYTKLTKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYKVDQLSVQYRTSFFIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEERTMSNLRGVESDV"
     gene            38957..40540
                     /locus_tag="O0305_000088"
     CDS             38957..40540
                     /locus_tag="O0305_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0273275.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            40527..41018
                     /locus_tag="O0305_000089"
     CDS             40527..41018
                     /locus_tag="O0305_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0273276.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLIAFNILNWLFWEI
                     LDNHLSIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            41022..41381
                     /locus_tag="O0305_000090"
     CDS             41022..41381
                     /locus_tag="O0305_000090"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCY0273277.1"
                     /translation="MIHGIGVDLIEIDRIQALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            41447..42595
                     /gene="alr"
                     /locus_tag="O0305_000091"
     CDS             41447..42595
                     /gene="alr"
                     /locus_tag="O0305_000091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0273278.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            42680..42850
                     /gene="mazE"
                     /locus_tag="O0305_000092"
     CDS             42680..42850
                     /gene="mazE"
                     /locus_tag="O0305_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCY0273279.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            42847..43209
                     /locus_tag="O0305_000093"
     CDS             42847..43209
                     /locus_tag="O0305_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0273280.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            43558..44559
                     /locus_tag="O0305_000094"
     CDS             43558..44559
                     /locus_tag="O0305_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCY0273281.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            44678..45004
                     /locus_tag="O0305_000095"
     CDS             44678..45004
                     /locus_tag="O0305_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCY0273282.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            45006..45485
                     /gene="rsbW"
                     /locus_tag="O0305_000096"
     CDS             45006..45485
                     /gene="rsbW"
                     /locus_tag="O0305_000096"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCY0273283.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            45460..46230
                     /gene="sigB"
                     /locus_tag="O0305_000097"
     CDS             45460..46230
                     /gene="sigB"
                     /locus_tag="O0305_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCY0273284.1"
                     /translation="MAKESKSANEISPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            46664..48814
                     /locus_tag="O0305_000098"
     CDS             46664..48814
                     /locus_tag="O0305_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCY0273285.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKKDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKAKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            48807..49262
                     /locus_tag="O0305_000099"
     CDS             48807..49262
                     /locus_tag="O0305_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCY0273286.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT        17481 a         7228 c         9323 g        15505 t
ORIGIN      
        1 cactttgcta tattcctttt ttatttttag gaagggtggg cttttatgaa agatttgaca
       61 atgttattag acgaattaaa agatatgtcc ttttttaata aaggagatat atgtttaatt
      121 ggatgttcaa catcggaagt cattggcgaa aaaataggga ctgtcggttc gatggaagtt
      181 gcagaaacta tttttaatgc attagatgtt gtgagtaaag agacaggcgt tacttttgct
      241 tttcaaggat gtgaacatat caacagagcg ataacaatag aaaaatctca gtataatcca
      301 ttaacaatgg aagaagtttc cgtcgttcca gatgtacatg ctggagggag tttggcaaca
      361 tacgcttttc agcatatgaa ggatccgatc gtggtcgaac atattactgt tccttgtgga
      421 attgatattg gtcaaacgtt aataggcatg catattaaac atgtatgtgt acctgtaagg
      481 acctcagtaa agcaagtagg acaggcaatt gtaactattg ccacatctag gccgaaaaag
      541 attggtggcg aacgtgctaa ataccaataa gtttttttga aagaagggga gattttatgt
      601 cttatatcac caagcaagat aaagttatcg cagaagcaat cgagagagaa tttcagagac
      661 aaaatagcaa catagagtta atcgcatcgg aaaattttgt atcggaagcg gttatggaag
      721 cacaaggttc agtgttgact aataagtatg ctgaaggcta tccaggacgc cgatattatg
      781 gtggctgtga gtttgtagat gttactgaaa gcatcgcaat tgatcgtgct aaagcattgt
      841 ttggagctga acatgtcaat gttcaaccac attcaggttc acaagcgaac atggctgttt
      901 acttagttgc attaggaatg ggcgacacag ttttaggtat gaatttgagt catggtggtc
      961 acttgacaca tggagcgcct gttaatttta gtggtaaatt ctacaatttc gttgaatatg
     1021 gagtagataa agacacagaa cgaatcaatt atgatgaagt tcgtaaatta gcgttagagc
     1081 ataagcctaa gcttattgtg gcaggagcat cagcatattc aagaacaatt gacttcaaaa
     1141 agtttaaaga aatcgcagat gaagtaaacg ctaagttaat ggtagacatg gcacatattg
     1201 caggattagt agcggcaggt ttacatccaa atccagtaga atatgctgat tttgtaacaa
     1261 ctacaacaca caaaacatta cgcggaccac gtggtggtat gattttatgt aaggaagaat
     1321 ataaaaaaga catagataaa acaattttcc ctggtattca aggtggacct cttgagcatg
     1381 ttattgcagc aaaagcagtt gcttttggag aagcgttaga aaataatttc aaaacgtatc
     1441 aacaacaagt ggttaaaaac gcaaaagttc ttgcagaagc attaattaat gaaggattta
     1501 gaattgtttc tggcggtaca gataatcact tagtagctgt tgatgtaaaa gggtctatag
     1561 gacttactgg taaagaagct gaagagactt tagattcagt tggtatcaca tgtaacaaaa
     1621 ataccattcc gttcgatcaa gaaaaacctt ttgtaacgag tggtatacgt ttaggtacac
     1681 ctgctgcaac aacgcgtgga tttgatgaaa aagcttttga ggaagttgca aaaatcatca
     1741 gtttagcatt gaaaaatagt aaagatgaag aaaaattaca acaagctaaa gaacgcgttg
     1801 cgaaattaac agctgaatat cctctatatc aataaacata cacaaagata ggagtaattt
     1861 ttatgagtaa agtacacgtt ttcgatcatc cattaattca acacaaacta agctatatta
     1921 gagatgtaaa tacaggtact aaagaattta gagaacttgt agacgaagtt ggtatgttga
     1981 tggcatatga agtaactaga gacttagagc ttcaggatgt tgatattgaa acgcctgtaa
     2041 ctaaaatgac ggctaaacga ttagctggta aaaaattagc tatcgttcca attttaagag
     2101 ctggtttagg tatgactgat ggtattttaa gtttagtacc tgcagccaga gttggacata
     2161 tcggtttata tcgagatccc gaaacattaa aggctgtaga gtattttgct aagttacctc
     2221 aagatattac tgaaagacaa atcattgttg ttgatcctat gttagcaaca ggcgcatcag
     2281 caattgaagc tattacttca ttgaagaaac gtggcgctaa aaatattcgc ttcatgtgtt
     2341 taattgcagc accagaaggc gtagaaaaga tgcatgaagc acatccagat gtagatattt
     2401 atattgctgc acttgatgaa aagctaaatg acaaagcata tatcacacca gggttaggcg
     2461 acgctggaga tagattgttt ggtactaaat agaagaatcg gagataagta gtttgatgaa
     2521 aaagattatg accatatttg gaacaagacc cgaagccata aagatggctc ctttagttaa
     2581 agctttggaa caagaaaaaa tgctcgagcc aattgttgtt gttacagcac agcatagaga
     2641 aatgcttgat tcagtattaa gtacctttga aatcaaacca aaatacgatt tgaatattat
     2701 gaaatcagga cagacacttt ctgagataac ttctaaatca atcactcaat tagaacaagt
     2761 gattcaatta gaaaaaccag acatggtact tgtacatggt gatacgatga cgacatttgc
     2821 aggtggttta gcggcatttt ataaccaagt gccgattgga cacgttgaag cagggcttag
     2881 aagttatgat aaatattcac cttttccaga agaggtgaat agacaattag ttggtgtatt
     2941 agctgactta cattttgcac ctactaaaaa tgctgcatcg catttgttaa gtgaagggaa
     3001 atattcagaa agtgttgttg tcactggtaa tactgcgatt gatgctatga aatatacagt
     3061 tgatgacaat tataaatcta acatcatgga taaataccat gacaaaaaat tcattcttat
     3121 gacagcacac cgacgagaaa atatcgggaa acccatggaa aatatattta aagcggttag
     3181 acgcttaata gatgagtata ctgatttagc actggtatat ccaatgcata aaaacccgaa
     3241 agttcgagag gtggctcaaa agattttagg tagtcatgac agaattgaat tgattgaacc
     3301 tttggacgta gtagatttcc ataattttgc taaaaaatct tattttattt tgacagattc
     3361 aggtggaatt caggaagaag cgccatcatt taataagcca gttttagttt tgcgaagtgt
     3421 tacagaacgt cctgaaggtg ttgaagctgg cacattaaaa gtcattggaa caaataagca
     3481 aaatgtgtat caagctgcaa aagaattaat cgatgatgag agattatatc atcaaatgtc
     3541 tgaagcatct aatccttacg gtgatggatt tgcttcagaa agaatagtta atcatattaa
     3601 gtattatttg aatttaatca ctgaaaaacc aagcgatttc tgaggaaaat agactttttg
     3661 tctaattgtg tctaaattgt gtctaactat gcttattttt ttacaacaat ttgaatagaa
     3721 ttgacaccac aaatcgccta tattatcatt tagtttgtat gagtggaacg gttttcatga
     3781 ttgatatcag tcacacggag gcatataaaa tgagtcgttt ctacaacatt tttaaacagt
     3841 tcattcaata ttatttttat ctaataataa tattgggagg attatacctt tatacacacc
     3901 atgcatttat cttaggatta atcattggtg ttactggttc tgcaatcaac acatgtattt
     3961 ttgaatgcta tttagctaaa gctaaaagac cagacactat gcatatttca actggaaata
     4021 tgtggcgata cttagttgca attattgcct gtatgatttg gtaccttaat aaagcgcatg
     4081 taagtatcat cggtataatt attggtttaa tgatttcata tgttgtagtt atcatacgtc
     4141 ctttactaaa ggtgagcaaa taaattaaga aagaggtgag attatggatc acaaatcccc
     4201 gctcgtgagt tggaatttat tcggttttga tatcgttttc aatttatcaa gtatattgat
     4261 gatacttgtt acggcgtttc ttgtttttct acttgctatc atttgtacgc gtaatttgaa
     4321 aaaaagacca actggcaaac aaaatttcgt tgaatggatt tttgatttcg tgaggggaat
     4381 cattgaaggt aacatggctt ggaaaaaagg tggtcaattc cacttcttag cagtaacgct
     4441 gattctgtac atttttatag ctaatatgtt aggtcttccg ttttctatag taacgaaaga
     4501 tcacacattg tggtggaaat caccgacagc tgatgcaaca gtgactttaa cgttgtctac
     4561 aacgataata ctgttaactc acttttatgg aattaaaatg cgtggtacga aacaatatct
     4621 taaaggttat gtacagccgt tttggccatt ggcaattatt aatgtttttg aagagttcac
     4681 ttcaacatta acgcttggtc tgcgtttgta cggtaacata tttgcaggtg agatactatt
     4741 aacattactt gctggcttat tctttaacga accagcatgg ggttggatta ttagtatccc
     4801 aggattaatt gtttggcaag cattttcaat atttgtagga acaatccaag catatatctt
     4861 tattatgctt tcgatggttt atatgtcaca taaagtggca gatgaacact aaaaatttca
     4921 ataattatat acaatcacag gaggaaatta aattatgaat ttaatcgcag cagcaatcgc
     4981 aattggttta tcagcattag gagcaggtat cggtaacggt ttaatcgttt caagaacagt
     5041 tgaaggtgta gcacgtcaac cagaagcacg tggtcaatta atgggtatca tgttcattgg
     5101 tgtaggttta gttgaggcat tacctatcat cggtgtagta attgcattca tgacatttgc
     5161 tggataatta acagataaaa gaggtcggga caaagcgcat aggacataat tcatgatgca
     5221 tatatagtaa tatctttgaa ctttattaaa tagttgagat atgaacgcac catgcctatc
     5281 gcataaattc agtaggtcct aacctcgtcg tttttttcta tataacacta gcgattattt
     5341 taatgaaagg agtgtcatga acccgtgact gaaacagcta acttattcgt tcttggtgca
     5401 gctggaggcg ttgagtgggg tactgtgatt gtacaggtcc taactttcat cgtgttactt
     5461 gcgttactta aaaagttcgc atggggtcca ttgaaagatg taatggataa acgtgaaaga
     5521 gatattaaca gagatatcga tgacgcagaa caagctaagt taaatgcaca gaaacttgaa
     5581 gaagaaaata aacaaaaact taaagaaaca caagaagaag ttcaaaagat tttagaagat
     5641 gctaaggttc aagcacgtca acagcaagaa caaattattc atgaagcaaa cgtacgtgca
     5701 aacggtatga ttgaaacagc acaaagtgaa atcaatagcc aaaaagaacg tgccattgca
     5761 gatattaata atcaagtatc tgaactatca gtgttaattg cttctaaagt tcttagaaaa
     5821 gaaatttctg aacaagacca aaaagcattg gttgacaagt atctaaaaga ggcaggcgat
     5881 aaataatggt aaaagtagct aacaagtatg ctaaagcatt atttgacgtg tcattagata
     5941 caaataattt agagactatt aatgaagaat taacagttat aaatgaagca gtaaaagata
     6001 aaattgagca attgagaatg gttgacagta atccaactca aacagcagaa caacgtcgtg
     6061 agttgattaa tggtgtattt actgacatta atccatacat taaaaatatg atgtacgtgt
     6121 tagcagataa tagacatatc tcattaatag ctgacgtatt caaggcgttc caaagcttat
     6181 ataacggaca ctacaatcaa gattttgcaa caattgagtc aacatatgaa ttgagtcaag
     6241 aagagttaga taagattgtc aaactagtaa ctcaacaaac gaagttatct aaagttattg
     6301 tagatacaaa aattaatcca gatttaattg gtggatttag agttaaagtc ggcacaactg
     6361 tattagatgg tagtgttaga aatgatcttg tccaattaca aagaaaattt agaagagtta
     6421 attaattata aagaggagtg acatagatgg ccataaaagc tgaagaaatc agtgcattac
     6481 ttcgctcaca aattgaaaat tatgagtcag aaatgtccgt aactgatgta ggtactgtat
     6541 tacaaattgg tgatggtatt gcattaattc acggattaaa tgacgttatg gctggtgagc
     6601 tagtagaatt ccataacggc gtacttggtt tagcccaaaa ccttgaagag tcaaacgtgg
     6661 gtgtggttat tttaggacca tacacaggta ttactgaagg tgacgaagtt aaacgtactg
     6721 gtcgtatcat ggaagtacca gtaggtgaag aactaatcgg aagagttgtt aatccattag
     6781 gacaacctat tgatggacaa ggaccgatta acacaactaa aacacgtcca gtagagaaaa
     6841 aagctactgg tgtaatggat cgtaaatcag tagatgagcc attacaaaca ggtatcaaag
     6901 caattgatgc tttagtacca attggtagag gtcaacgtga gttaatcatc ggtgaccgtc
     6961 aaacaggtaa aacaacaatt gcaattgaca caattttgaa ccaaaaagat caaggtacga
     7021 tttgtatcta tgttgctatt ggtcaaaaag attcaacagt aagagcaaat gttgaaaagt
     7081 taagacaagc aggcgcttta gactacacta ttgttgtagc agcatcagct tctgaacctt
     7141 ctccattatt atatattgca ccatattcag gtgtaacaat gggtgaagaa ttcatgttta
     7201 acggtaaaca tgttttaatc gtttatgatg atttaactaa acaagcagca gcttatcgtg
     7261 agttatcatt attattacgt agacctccag gtcgtgaagc atacccaggt gacgtattct
     7321 acttacatag tagattatta gaaagagcag caaaattaaa cgatgactta ggtggcggtt
     7381 caattactgc attaccaatt atcgaaacac aagctggtga tatttcagct tatgtaccaa
     7441 caaacgttat ttcaattaca gatggacaaa tcttcttaca atctgattta ttcttctcag
     7501 gtgtaagacc agcgattaat gccggacaat ctgtatctcg tgttggtgga tctgcacaaa
     7561 ttaaagcaat gaagaaagtt gctggtacgt tacgtcttga cttagcgtca tacagagaac
     7621 ttgaatcatt tgcacaattc ggttcagacc ttgatgaatt tactgcaagt aaattagaac
     7681 gtggtaaacg tactgttgaa gtcttaaaac aagatcaaaa caaaccatta ccagtcgaac
     7741 accaagtgtt gattatttat gcattaacaa aaggatattt agatgatatt ccagttgtag
     7801 atatcacacg ttttgaagac gagttaaacc actgggcaga atcaaatgct actgaactgt
     7861 taaatgaaat cagagaaact ggtggcttac cagatgctga gaagtttgac acagcaatta
     7921 acgaattcaa aaaaagcttt agcaaatctg aataataaac aagtttagta taaggtggtg
     7981 agatagtggc ttctcttaaa gaaatagata ctcgaataaa atcaaccaaa aaaatgaagc
     8041 agattacgaa agcgatgaac atggtatcaa gttcaaaact tcgtagagct gaaaaaaata
     8101 caaaacaatt cacaccatat atggataaaa tgcaagatgc aattactgca gttgcagggg
     8161 caagtagcaa tacaaaccat ccaatgctaa gacctcgtaa aattactaga agtggatatt
     8221 tagttatcac gagtgataaa ggtttagcag gtgcatatag tgcaaacgtg cttaaaaaat
     8281 tgattactga tattgaagcg aaacatcaag atagtagcga atacagtatt gtagttttag
     8341 ggcaacaagg tgttgatttc ctaaaaaata gaggttatga cattgagtat tctcaagtag
     8401 acgtacctga tcaaccttct ttcaaatctg ttcaagcact agctaaccat gctatagact
     8461 tatacagtga agaagaaatt gatgaattaa atatatacta tagtcattat gtcagcgttc
     8521 ttgaaaacaa gcctacatct agacaagtat taccattatc tcaagaggat tctagtaagg
     8581 ggcatggtca tttgtcttct tatgaatttg agccagataa agaatctatc ttaagtgtaa
     8641 tcttgcctca atatgttgag agtttgattt acggaacaat attagacgca aaagcaagtg
     8701 agcatgcaac acgtatgact gcgatgaaaa atgccactga taatgcaact gaacttattg
     8761 atgacttatc attagaatat aacagagcga gacaagcaga aattacgcaa caaattactg
     8821 aaattgttgg tggttccgca gcgcttgaat aatatttaaa ggaggaaaat agcatgggaa
     8881 ttggccgtgt aactcaagtt atgggtcctg taattgatgt tcgatttgaa cataacgaag
     8941 ttcctaaaat taataacgcc ttggttattg atgtgcctaa agaagaaggt acaatacaac
     9001 taacattaga agttgcgctg caattaggtg acgacgttgt tcgtacaatt gcgatggatt
     9061 caactgatgg tgtccaaaga ggcatggatg taaaagatac aggcaaagaa attagtgtac
     9121 ctgttggtga cgaaacatta ggtcgtgtat ttaatgtact aggtgaaaca attgacctta
     9181 aagaagaaat tagtgattct gttcgccgcg atcctatcca tcgtcaagca ccagcattcg
     9241 atgaactttc aacagaagtt caaattttag aaacaggtat taaagtagta gatttactag
     9301 caccttatat taaaggtggt aaaatcggat tgttcggtgg tgccggtgta ggtaaaacag
     9361 tattaatcca agaattaatt aacaacatcg ctcaagagca cggtggtatt tctgtattcg
     9421 ccggtgtagg tgaacgtact cgtgaaggta acgatttata cttcgaaatg agtgacagtg
     9481 gtgtaattaa gaaaacagcc atggtattcg ggcaaatgaa tgagccacct ggtgcacgta
     9541 tgcgtgttgc attatctggt ttaacaatgg ctgaatattt ccgtgacgaa caaggtcaag
     9601 acgtattatt attcatcgat aacattttca gatttacaca agctggttct gaggtatctg
     9661 cattattagg tcgtatgcct tctgcagtag gttaccaacc aacacttgct actgaaatgg
     9721 gacaattaca agaacgtatt acgtctacaa caaaaggatc agttacttct attcaagcgg
     9781 tattcgtacc tgccgatgac tatactgacc cagcgcctgc gacagcgttt gcccatttag
     9841 atgcaactac aaacttagaa cgtaaattaa ctgaaatggg tatttatcca gccgtggatc
     9901 cattagcgtc tacatcaaga gcattggaac catcaattgt aggtcaagaa cattatgaag
     9961 tagctcgtga tgtacaatca acacttcaaa aataccgtga attacaagat atcattgcta
    10021 tcttaggtat ggacgaatta tctgatgaag ataaacaaac agttgaacgc gcacgtagaa
    10081 ttcaattctt cttatctcaa aacttccacg tagcggaaca atttactggt caaaaaggtt
    10141 cttatgtacc tgttaagaca acagttgcaa actttaaaga tatcttagat ggtaaatatg
    10201 accatattcc agaagatgca ttccgtttag ttggtagcat ggatgatgtt attgcaaaag
    10261 ctaaagatat gggtgttgaa gtataacaat taggaggaat ggataatgaa tacattaaac
    10321 ctagatattg tcactcctaa tggttctgtt tacaatcgtg ataatgttga actcgttgtt
    10381 atgcaaacaa cagctggtga gataggtgtc atgagtggac atattccaac tgtagctgct
    10441 ttaaaaacag gctttgtaaa agtgaaattt cacgatggaa ctgaatatat tgctgtaagc
    10501 gatggctttg ttgaagttag aaaagataaa gtttcaatca ttgttcagac tgcagaaact
    10561 gcaagagaaa ttgatgttga aagagctaaa ttagccaaag caagagcaga gtctcacttg
    10621 gaaaatgatg acgacaatac tgatattcat agagccgaaa gagctttaga gagagcaaat
    10681 aaccgtttgc gtgtggctga attaaaatag taaataaagg gtcgaagatg tgatttcata
    10741 tcttcgaccc ttttttgaat tatattgatt taaagataca aaacatgaga ggggggaagg
    10801 aattgataaa gaaccattaa agatttatga tgtagtggtt ctttatcatt aaacacagct
    10861 aatgtgtatt taaaaatagg aatacatgag taaaactcat gtataagaaa tactaatttc
    10921 taaagaaaaa gtatttcttt atgttggggc cccgtcaact actgccaaat acaacactat
    10981 agagtctaga cattgattta tgtccgactc ccaagaatag ttttactttt ttacaatcac
    11041 taatagattg ctaaaatcaa aatttccttc accactatct acagtcgaca tttcattttt
    11101 tgaaattatc tacatttttt cataccaaga tattttatag ttatgatatt tatgtaaaaa
    11161 gaattatata gtaagttagc ttaaacttta ctaaaaacgg gtattaaact ttgtatcatt
    11221 atttaaattt ttcatgtaca atgtaataca gtaatcttat gaggtgataa aatggattat
    11281 atcggacaat atgcagttat ccatttagtg ttacatgttg tatgtatttg tattgcctat
    11341 tgggctttac aatcaattag attagatcaa ttttttaaaa aaggatacgc cactcaatta
    11401 caagtgtgta tgatatttgt tgctatttta ttaggcactg cagtaagcaa ttttattgta
    11461 gatttgttac aatactcgac gcaggtaaaa tatttaataa aataagtcta actctatgat
    11521 ttgtaatcaa aactagatat aattaaataa tgacttaaaa taattttaaa atagggaaat
    11581 gtaaagtaat aggagttcta agtggaggat ttacgatgga taaaatagta atcaaaggtg
    11641 gaaataaatt aacgggtgaa gttaaagtag aaggtgctaa aaatgcagta ttaccaatat
    11701 tgacagcatc tttattagct tctgataaac cgagcaaatt agttaatgtt ccagctttaa
    11761 gtgatgtaga aacaataaat aatgtattaa caactttaaa tgctgacgtt acatacaaaa
    11821 aggacgaaaa tgctgttgtc gttgatgcaa caaagactct aaatgaagag gcaccatatg
    11881 aatatgttag taaaatgcgt gcaagtattt tagttatggg acctctttta gcaagactag
    11941 gacatgctat tgttgcattg cctggtggtt gtgcaattgg aagtagaccg attgagcaac
    12001 acattaaagg ttttgaagct ttaggcgcag aaattcatct tgaaaatggt aatatttatg
    12061 ctaatgctaa agatggatta aaaggtacat caattcattt agattttcca agtgtaggag
    12121 caacacaaaa tattattatg gcagcatcat tagctaaggg taagacttta attgaaaatg
    12181 cagctaaaga acctgaaatt gtcgatttag caaactacat taatgaaatg ggtggtagaa
    12241 ttactggtgc tggtacagac acaattacaa tcaatggtgt agaatcatta catggtgtag
    12301 aacatgctat cattccagat agaattgaag caggcacatt actaatcgct ggtgctataa
    12361 cgcgtggtga tatttttgta cgtgatgcaa tcaaagaaca tatggcgagt ttagtctata
    12421 aactagaaga aatgggcgtt gaattggact atcaagaaga tggtattcgt gtacgtgctg
    12481 aaggggaatt acaacctgta gacatcaaaa ctctaccaca tcctggattc ccgactgata
    12541 tgcaatcaca aatgatggca ttgttattaa cggcaaatgg tcataaagtc gtaaccgaaa
    12601 ctgtttttga aaaccgtttt atgcatgttg cagagttcaa acgtatgaat gctaatatca
    12661 atgtagaagg tcgtagtgct aaacttgaag gtaaaagtca attgcaaggt gcacaagtta
    12721 aagcgactga tttaagagca gcagccgcct taattttagc tggattagtt gctgatggta
    12781 aaacaagcgt tactgaatta acgcacctag atagaggcta tgttgactta cacggtaaat
    12841 tgaagcaatt aggtgcagac attgaacgta ttaacgatta attcagtaaa ttaatataat
    12901 ggaggatttc aaccatggaa acaatttttg attataacca aattaaacaa attatacctc
    12961 acagacagcc atttttatta attgataaag tagttgaata tgaagaaggt caacgttgtg
    13021 tggctattaa acaagtatca ggaaacgaac cattctttca agggcatttt cctgagtatg
    13081 cggtaatgcc aggcgtatta attactgaag cgttagctca aacaggtgcg gtagctattt
    13141 taaatagtga agaaaataaa ggtaaaatcg ctttatttgc tggtattgat aaatgtcgtt
    13201 ttaaacgtca agtagtacct ggtgatactt taacgttgga agtagaaatc actaaaatta
    13261 aaggaccaat aggtaaaggt aatgctaaag ctactgtcga tggtcaactt gcttgtagtt
    13321 gtgaacttac atttgcaatt caagatgtaa aataaaacaa aaaaaacatt caaagattta
    13381 atgtgttggc ataatctttg aatgtttttt attttactct tctaattttt catcctttaa
    13441 ctttggttta gactgcatca ttcgattaaa tgattttttt aattcttcac cagataatcc
    13501 atcatcaata agttgttcta ataaactttc agcatactgt tgacgtaaag tgtaaatatg
    13561 acattcaaat acgatagaca ttgttgtttt aaaattttta atacttttca cagtagattc
    13621 aaataaagct ggatcattgg ctggacgcgt gatgacgaca cgaatatcca ataactgttc
    13681 attgtcttga tattccttaa atgtttcata atgctcatta gaaatgacaa tttcaagcaa
    13741 ccaacccgtg ccactttcct ctttattaat tattacgcca tcttctaatt catattctat
    13801 aattctgccg tgttcattca caatttgaaa ccttactgct ttaaaagttt tcatattccc
    13861 acatcccatt agtttaatat ttatgatttt tgaattattt agaagattta aggtcatttc
    13921 atgactaatt tctttaaata taacaaataa cgagatgcta tagctattaa ttatataaaa
    13981 tttgccattt tgaccatttt taagctagta attaagatat aggcatagga ggtgaatcac
    14041 acactacatg ctaaataaaa tcgtaattgt cgggagactg acgaaagacg cacaaatatt
    14101 tgaaaaggag gatagaaaaa ttgcaacgtt ttgtgttgca acgcaccgaa attataaaga
    14161 tgaaaatgga gaaatcgtct gtgattactt attctgtaaa gcatttggca agttagcttc
    14221 taatatagaa aaatatacta atcaaggtac attggttggt ataactggtc aaatgagatc
    14281 aagaaagtat gataaagacg gacaaacaca ctttgtcact gaattatatg ttgaaacaat
    14341 aaaatttatg tcccctaaat cccaaaataa tgaaattctc tcagatagta tttagatatt
    14401 gactctcaaa atatagataa tcatgactta ttagaaattt aaatccaatt atcctaaaca
    14461 tccttaatat acatttaatc catcctaaat tttttataga tctaattaac ttgaaatata
    14521 ctcacttaaa aacgacttac atgactagta ctgttgtgta ggtcgtcttt ttgtgtagtt
    14581 tttctaatag aaaatagaca tagattgcaa aacgattaca taaaaaattg caaataaaaa
    14641 aacattttta ctgtcatgac aattctcgat agaatcatat ctgaaattat gggggatttc
    14701 gttgtaatca ctgtgtaaaa aacaaggcta tttttttatt ttgttgtaac ctgcacggat
    14761 tttatagatg ttacagtagt ctctgtaaaa cttttagaga aacaaattac ttgtaggagg
    14821 attttaagat tatgaagaaa acattactcg catcatcatt agcagtaggt ttaggaatcg
    14881 tagcaggaaa tgcaggtcac gaagcccatg caagtgaagc ggacttaaat aaagcatctt
    14941 tagcgcaaat ggcgcaatca aatgatcaaa cattaaatca aaaaccaatt gaagctgggg
    15001 cttataatta tacatttgac tatgaagggt ttacttatca ctttgaatca gatggtacac
    15061 actttgcttg gaattaccat gcaacaggta ctaatggagc agacatgagt gcacaagcac
    15121 ctgcaactaa taatgttgca ccatcagctg ttcaagctaa tcaagtacaa tcacaagaag
    15181 ttgaagcacc acaaaatgct caaactcaac aaccacaagc atcaacatca aacaattcac
    15241 aagttactgc aacaccaact gaatcaaaat catcagaagg ttcatcagta aatgtgaatg
    15301 ctcatctaaa acaaattgct caacgtgaat caggtggcaa tattcatgct gtaaatccaa
    15361 catcaggtgc agctggtaag tatcaattct tacaatcaac ttgggattca gtagcacctg
    15421 ctaaatataa aggtgtatca ccagcaaatg ctcctgaaag tgttcaagat gccgcagcag
    15481 taaaattata taacactggt ggcgctggac attgggttac tgcataagcc atttatgcat
    15541 agctaattaa tagttatata agtaactttt agattggaat atatcgggga attctcgata
    15601 tgttccggtt tttttatttc gcaaatatat gtcattatag aattattatt taaagataat
    15661 aatacaactg agcaacaata attgaaaatg ttgaaaccgt ttgctaagat taatttgcaa
    15721 ttgaatacta aggaactact aggggagcct aatgatatgg ctgagatgaa ttgttcagac
    15781 ccttatgacc tgatttggtt agtaccaacg taggaaagta gttattgaag tgatggggct
    15841 agtatagcaa cattgccatc aaactacttt catgttggaa gtagtttttt tatttaggga
    15901 ggatttttta caatggaatt ttcacaaaaa ttgtaccaag ctgcaaagcc aatcattaat
    15961 gatatttatg aggatgactt tattcagaag atgttatccg gagatatagg agctgacgca
    16021 ttacgccatt acttaaaagc tgatgctgcg tatttaaaag aatttacaaa cttatatgct
    16081 ttgttaattc caaaaatgaa tagcatgaat gacgtaaaat ttttagtaga gcaaatagaa
    16141 tttatggttg aaggtgaagt acttgcgcat gatattttag cgcaaattgt tggtgaatca
    16201 tacgaagaaa tcattaaaac aaaagtttgg cctccaagtg gcgaccatta cataaaacat
    16261 atgtatttcc aagcgcatag tcgtgaaaat gctatttata caattgctgc aatggcacct
    16321 tgtccatata tttatgcaga attagctaag cgctctcaaa gtgatcataa gcttaataga
    16381 gaaaaagata cggcaaaatg gtttgatttt tatagtactg aaatggatga cataattaac
    16441 gtatttgagg cattaatgaa taaattagcc gaatcaatgt ctgacaagga attggaacaa
    16501 gtaaagcaag tatttttaga aagttgcata catgagcgtc gatttttcaa tatggctatg
    16561 acactagaac aatgggaatt tggaggaaaa gtaaatgatt aaacctaaaa tagcattaac
    16621 cattgcaggt actgatccaa caggtggtgc cggcgtaatg gctgatttaa aatcatttca
    16681 ttcatgtggt gtatatggta tgggcgtcgt tacaagtatt gttgctcaaa atacattggg
    16741 cgtacaacat attcataatt taaatcatca atgggtagat gaacaacttg atagtgtctt
    16801 caatgatacc ttacctcatg ctattaaaac ggggatgatt gctacagcag atactatgga
    16861 aacgattcgt cattatttaa tgcaacatga atctattcca tatgtaattg atcctgttat
    16921 gttggcgaaa agcggtgatt cattaatgga taatgacaca aagcaaaact tgcagcatac
    16981 gttattgcca ttagctgacg tagtaacacc gaatttacca gaagctgaag aaataacggg
    17041 actaaccatt gatagtgaag aaaaaattat gcaggctggc cgcatcttta ttaatgagat
    17101 tggtagtaaa ggtgtcatca ttaaaggcgg tcattcaaat gatactgata tagcaaaaga
    17161 ttatttattt actaacgaag gtgttcaaac atttgaaaat gaacgattta aaacaaaaca
    17221 tacgcatgga acagggtgta cattttcagc agttataacg gcagaacttg caaaaggtag
    17281 accattattt gaggctgtac acaaggctaa aaagtttatt tcaatgagta tacaatatac
    17341 gcctgaaatc ggccgtggta gaggtccagt gaatcatttt gcatatttaa agaaagaggg
    17401 attagacgat gaattatcta aataaaatac gtattgaaaa cccattaaca atttgttata
    17461 caaacgatgt agttaaaaat tttacagcga atggtttatt aagtattggt gctagccctg
    17521 caatgagtga agctcccgaa gaagctgaag aattttacaa agttgcacaa gcgctattaa
    17581 tcaatatcgg tactttaaca gcagaaaatg aacaagatat tattgcgatt gctcaaacgg
    17641 caaatgaggc aggcttacct attgtatttg accctgtagc tgttggtgct tctacatatc
    17701 gaaagcaatt ttgtaaatta ttattgaaat cagcgaaagt atcagtaatt aaaggcaatg
    17761 catctgaaat attagcgttg attgatgata cagcaactat gaaaggtaca gatagtgatg
    17821 ctaatcttga tgcggttgca atagcgaaaa aggcttacgc aacatataaa actgcaatag
    17881 taatcacagg taaagaggac gttattgttc aagataataa agccttcgta ttagctaatg
    17941 gatctccatt attagcacga gtaactggag ctggttgttt attaggaggc gttattgctg
    18001 gatttttatt tagagaaaca gaaccagaca tagaagcgtt aattgaagcg gtaagcgtat
    18061 ttaatattgc tgctgaggta gctgctgaaa atgaaaattg tggtggtcct ggtacgtttt
    18121 caccattgtt gcttgatacg ttatatcatt taaatgaaac aacctatcaa caacggattc
    18181 gtattcaaga ggtggaataa tatgtttaac caatcgtatc taaatgtgta ttttatttgt
    18241 gggacctccg atgttccgag tcatcgaact attcatgaag tattagaagc agcattaaag
    18301 gcagggatta cacttttcca atttcgagaa aagggtgaat cagctttaaa aggaaacgat
    18361 aaacttgtat tagctaaaga attacagcat ctctgtcatc aatatgatgt gccttttatc
    18421 gttaatgatg atgtatcctt ggcaaaagaa attaacgcgg atggtataca tgtcggtcaa
    18481 gatgatgcga aagtcaaaga gatagcacaa tatttcactg ataaaattat tggacttagt
    18541 attagtgatt tagatgagta tgcaaaatct gatttaactc atgttgatta tataggtgtt
    18601 ggaccaatct atcctacgcc gtcgaagcat gacgcgcata taccagtagg tccagaaatg
    18661 attgcaacgt ttaaagaaat gaatccgcaa ctaccgattg tagctattgg cggtatcaat
    18721 actaataatg tggcgccgat tgtagaagca ggtgccaatg gaatttcagt catttcagcc
    18781 atttctaaaa gtgaaaatat tgaaaagact gttaatcgat tcaaagattt ttttaataat
    18841 tagttaaatt tttccactta ttgtttcata cgtactcatt aatgtgatag aataaatcct
    18901 atgtgaatat aatgatagag gtggaaaaat gaagaaaaaa gcgttactac cattattttt
    18961 aggtattatg gtctttttgg ctggttgtga ctattctaaa cctgaaaaac gtagtgggtt
    19021 tttctacaat acattcgtag atccaatgaa aaatgtattg gattggttgg gaaataactt
    19081 attaaacgac aattatggtt tagctattat tatccttgta ttggtaattc gtattatttt
    19141 attaccattc atgttgtcaa actataaaaa tagtcatatg atgcgtcaaa aaatgaaagt
    19201 tgcaaagcca gaagttgaaa aaattcaaga aaaagtgaaa cgtgcgcgta cacaagaaga
    19261 aaaaatggct gcaaaccaag aattaatgca agtatataaa aagtatgaca tgaacccgat
    19321 taagagtatg ttgggttgtt taccaatgct aatccaatta ccaatcatca tgggattata
    19381 ctttgtactt aaagaccaac ttgtagatgg tttgtttaaa tatccacact tcttatggtt
    19441 cgatttagga cgtcctgata tttggattac aattattgcc ggtgttttat actttatcca
    19501 agcatatgta tcaagtaaaa cgatgccaga cgaacaacgt caaatgggtt acatgatgat
    19561 ggtcatttca ccaattatga ttatctggat ttcattaagc tcagcatcag cacttggttt
    19621 gtactggtca gtcagtgcgg cgttccttgt agttcaaaca cactttgcga acatttatta
    19681 tgaaaaagtc gctaaaaaag aagtacaacc tttcattgaa gcgtatgaaa gagagcacaa
    19741 cggcggcagc aataaaaaag gcaaaaacac acaagttgtg tctaaaaaga aaaagaaata
    19801 accttagcaa gtccaaataa tgtgcaccct aaaagttgaa gataatttta actttatagg
    19861 gcgcacatta tttttcgctt caatagcgaa acttagtgtc acattataca atatgtgata
    19921 aatagaaaca gcagtaagat gatttcaaat gtgaaagtca tcctactgct gtttttattt
    19981 gaaataatat taacgagttt tcagtatgta aaaagtggtt aataacagag ttgtcattct
    20041 tcatcgcaat gtcatcttga cacaatgatt catcagtgta gcagtatatg tcgcaaattt
    20101 ccacagcaat tcacttatac atgtaacagt gtgaaataat aacgtcacaa cggcataata
    20161 cacattgcaa cgtcccaaca acatatcata gaatcgtaac ggcatgacat caacaccaaa
    20221 cctggctata acaccataac agcgcaacgc cacatcatag cactacatca agtcgcatcg
    20281 tagcaccagc acatcaaagt aacatggcat caacatcaaa ccggtctata acaccataac
    20341 accgcaatgc cacatcatag cactacatca agtcgcatcg tggtaccagc acatcaaagt
    20401 aacatggcat caacgccaaa cctgtctata acaccataac accgcaatgc cacatcatag
    20461 cactacatca agacgcatcg tggtaccagc acatcaaagt agcatgacat gtcaccaagc
    20521 tcccacaaca acacatcaaa gtaacatggc atgacaccaa gcttccacaa caacacgcca
    20581 ttacatcgca tcatcatact acaacttcaa tgtctagtcg tgacaattcc attccgtaaa
    20641 aaactgtttc aagtacatca tcataaagtc gtgacgttct ttagcaatca tcttcgccgt
    20701 atctgtatgc attaaagatt ctaacttaag taatttttca aagaaatgtt taattgcaga
    20761 tggtggcaac tgttcaacta aatcatcatt aatcttatct agtgacatat gttctgtcca
    20821 cataggttca ccaaagtgtc ctgcaaattg aaatgttcgt gcaacaccta tagcgcctat
    20881 agcatcaaga cgatctgcat ccctgacaat ttgaccttct aaagataaag tgacatgatc
    20941 atttttgcca ttgcgatagc tcatattatt aataataaat aaaatgtgct cttgatcttc
    21001 ggttgatagt gataaagaag ataaaaatga cttcaattca acatattgtt tgttagcatc
    21061 tacaactttt tcgtcaacgg tatcatgaag caaacatgcg agttcaatga ctaaagtatc
    21121 attaacacct tcattttcag cgattgattt agctaaagca gtgacacgat atacatgcgc
    21181 aatatcatgg cctgtataat cattttgatg aatagattcc atatattttt tggcagcact
    21241 aatgacgtct gattgttgca ttaaagttac actcctcata tttctatttt ataagatagg
    21301 tgacaataat tgtgaaatac cttctttaaa tttaatccaa agacttcgct tagcataacg
    21361 tgcttttgtt aattcagaag atactgctaa atcatctata aaagcttgtt ttaatttttt
    21421 ggcaatttgt tggtcataaa taaaagcgtt gacttcgaaa ttcaatgtga aactgcgatg
    21481 gtccatatta gctgttccca cacttgcaat ttcatcatct ataacaagtg tttttgagtg
    21541 taaaaagcca ttgtcgtagt gaaatacttt aacaccggca tctaataagg atgctgcatt
    21601 ttttaaagta gcccaaaaaa caaacggatg gtcaggttta ttaggaatca tgatattgac
    21661 atcaacacca cctaatgccg caattttaat agaatctaaa aaggcttgat caggtatgaa
    21721 atagggagat tgaatataaa tcgatttttt agcagatgaa atcattttca aatagccgta
    21781 tttaatctgt tcccattctt cgtcaggacc actagaagct atttgaacgc caattgttcc
    21841 accagaattt acatctggga aataacgatc atcataggag atgtggtcac gtgtggcttg
    21901 tgaattccaa tctagaataa atcgtaattg caatgcattc actgcatccc cgacaattcg
    21961 taaatgcgta tctcgccaat agccgaattt ttttgattta cctaagtact catcaccaac
    22021 attaaaacca ccaacatatc caatttgccc atctattaca acaatttttc gatggtttcg
    22081 attgttcata cgcaagttaa ttaaaggtaa ttttgatggg aaaaatgctt cagcatgtcc
    22141 acctttattg cgaaacgggc gtaagccttt tttacgcagt ccacgagaac ccatgtcatc
    22201 ataaagaatt ttaacttcta caccttgttc cgctttttta ccaagttcat ttaaaatggt
    22261 acgacctaat tcatcatttt gaataatata gtactgaaaa tgaatataat cagtagcatt
    22321 acggatgtct tgtattaggt catcaaattt ttcttggccg tctgtgtata tttttaaatc
    22381 gttgtctgtt gttaaaaatg ctgcgttatt atataacaac atttgaatca tttctttaaa
    22441 ttttacaatt tgataattgg aatttgaaaa gttttcattt tttaaagcag ctaattgctc
    22501 atcaacgatt aactctaatc cttttttatc ttccttatca attttgaaaa tttggtcacg
    22561 ttgaatttgt cgtcctaata gtaagtataa aatgaagccg aataaaggca agaaaactaa
    22621 gactagtaac caagcccaga tagaattggc agaacgtctt tccatgaaaa taatggtaaa
    22681 ggcgaataat aagtttaaaa taaatgcacc aataaatatt gaatttaaaa taatattaga
    22741 atgcttgagt gcaatggata ataactctat catttgaaac ctcccatcga aaatcttaaa
    22801 cgagtagtat atcttattaa ttgtatcatg aagcaattta aatatcatga caatttagtt
    22861 tgaaaaacac actttgtgtt gacgttgtgt tttatgggat atagtatagt aaggaatgta
    22921 aatgaaggag tgaatgctat gactgaacaa gataatgcac atcattctga acaaataaaa
    22981 acgaatctta aatcacgttt aaatcgaatt gaaggacaag tgagagcgat taatcgcatg
    23041 attgaagaag atgtctattg tgatgatgtc cttacgcaaa taagagcgac acgttcggcg
    23101 ttaaacagtg ttgcgataaa gttattagaa caacatatga aaagttgtat tatgaataaa
    23161 gttaatcaag gtgctcagga agaggcaatg gaagagttat tagtgacttt tcaaaaattg
    23221 attaaagact aaaggagttt aagatgatac atcaaaatac gatttacaca gcgggaattg
    23281 aaacagaaga acaagtaagt caattgacag aacgcatttc aaatatgata ggtgttcatc
    23341 aagtgaatat taatataata gatggtcaag taactgtatc gtatgagaca ccagcaaatt
    23401 tgaatagtat tgaaaaagaa atctatgatg aaggatacaa aattgtattt tagggtataa
    23461 tgtagagtgc gctatagatt ttaattttga aaataaatta aaaattttgt aaatgatgta
    23521 gtaaaggtat gtcgaatagg gtaagataat attgaaagcg atccaaacga ggtgataata
    23581 atgaaacgtc ctgaaaagat tcaaaatgta gtcaaactat tgtcatcatt aggtgtgaat
    23641 attaaaaaaa ctaaatctcg tttagacatt attaatactt tgccagcatc taataaagta
    23701 agtcacgaat taaaataatt gactgtgatt gtgaatcaca caccgttaaa gctatcgtcg
    23761 gactattcga tgatagcttt tttgactgga tttactaaaa tgaaagcaag tactatataa
    23821 caaaatccct ctttagagtg tacattttac aatgtgtact tcaaagaggg attgttatta
    23881 tcgagtaata tctatatatg acattttaaa tcgacgacgt aaatagacta tataacgtca
    23941 tcttgaaaag ttatgatatt tgaaactcaa aatagtttaa attaattact ttttggatgg
    24001 tataaatcga catatcgttt tggttcatga taataaattg ataagactat acctattcca
    24061 gtcatcatac tccatagcgc actaccacca taactaataa atggtaatgg aataccagtg
    24121 attggtaaca actgaattgt cataccaata ttttgtaaaa tatggaacac aagtaaagtg
    24181 acgaaaccaa cgataaagat tttgttaaat tgatcttcaa ttttcgcagc taatcttatt
    24241 agatggaaga ttaaaaataa aaatattaag atcaatatga cagaaccgat aaagccaagt
    24301 tcctctccaa tcactgaaaa gataaagtca gtatgatttt caggtatata aacttcaccg
    24361 tgattgtatc ctttacctag taactgtcca gaaccgatag ctttaagtga ttcagttaaa
    24421 tgatagccat caccactact atatgtatag gggtcaagcc atgaattgat tcgtcccatt
    24481 tgatacagtt ggacacctaa taaattttca attaatgcgg gtgcatatag aatacctaaa
    24541 atgactgtca ttgcaccaac aatacctgta ataaagatag gtgctaagat acgccatgtt
    24601 ataccactta ctaacatcac acctgcaata atagcagcta atactaatgt agttcctagg
    24661 tcattttgca gtaatattaa aatacttggt actaacgaga caccaataat tttgaaaaat
    24721 aataacaaat cactttggaa tgatttattg aatgtgaatt gattatgtct agaaacgaca
    24781 cgcgctaatg ctaaaattaa aataattttc atgaattcag atggctgaat actgataggg
    24841 ccaaacgtgt accaactttt ggcaccattg ataataggtg taataggtga ctcaggaata
    24901 acgagcaagc ctattaataa tagacagatt aagaaataca ataaatatgt ataatgttta
    24961 atctttttag gtgaaataaa catgatgata cctgcaaaaa ttgcacctaa aatgtaataa
    25021 aaaatttgtc tgataccgaa attagcactg tattgaccac cgcccattgc cgagttaata
    25081 agcagaacac tgaaaattgc taaaacagct atagtggcta ctaataccca gtctactttg
    25141 cgaagccaat gcttatccgg ctgttgacga gatgaataat tcattgcaaa ctccttttat
    25201 actcactaat gtttatatca attttacatg actttttaaa aattagctag aatatcacag
    25261 tgatatcagc tatagatttc aatttgaatt aggaataaaa tagaagggaa tattgttctg
    25321 attataaatg aatcaacata gatacagaca cataagtcct cgtttttaaa atgcaaaata
    25381 gcattaaaat gtgatactat taagattcaa agatgcgaat aaatcaatta acaataggac
    25441 taaatcaata ttaatttata ttaaggtagc aaaccctgat atatcattgg aggaaaacga
    25501 aatgacaaaa gaaaatattt gtatcgtttt tggagggaaa agtgcagaac acgaagtatc
    25561 gattctgaca gcacaaaatg tattaaatgc aatagataaa gacaaatatc atgttgatat
    25621 catttatatt accaatgatg gtgattggag aaagcaaaat aatattacag ctgaaattaa
    25681 atctactgat gagcttcatt tagaaaatgg agaggcgctt gagatttcac agctattgaa
    25741 agaaagtagt tcaggacaac catacgatgc agtattccca ttattacatg gtcctaatgg
    25801 tgaagatggc acgattcaag ggctttttga agttttggat gtaccatatg taggaaatgg
    25861 tgtattgtca gctgcaagtt ctatggacaa acttgtaatg aaacaattat ttgaacatcg
    25921 agggttacca cagttacctt atattagttt cttacgttct gaatatgaaa aatatgaaca
    25981 taacatttta aaattagtaa atgataaatt aaattaccca gtctttgtta aacctgctaa
    26041 cttagggtca agtgtaggta tcagtaaatg taataatgaa gcggaactta aagaaggtat
    26101 taaagaagca ttccaatttg accgtaagct tgttatagaa caaggcgtta acgcacgtga
    26161 aattgaagta gcagttttag gaaatgacta tcctgaagcg acatggccag gtgaagtcgt
    26221 aaaagatgtc gcgttttacg attacaaatc aaaatataaa gatggtaagg ttcaattaca
    26281 aattccagct gacttagacg aagatgttca attaacgctt agaaatatgg cattagaggc
    26341 attcaaagcg acagattgtt ctggtttagt ccgtgctgat ttctttgtaa cagaagacaa
    26401 ccaaatatat attaatgaaa caaatgcaat gcctggattt acggctttca gtatgtatcc
    26461 aaagttatgg gaaaatatgg gcttatctta tccagaattg attacaaaac ttatcgagct
    26521 tgctaaagaa cgtcaccagg ataaacagaa aaataaatac aaaattgact aactgaggtt
    26581 gttattatga ttaatgttac attaaagcaa attcaatcat ggattccttg tgaaattgaa
    26641 gatcaatttt taaatcaaga gataaatgga gtcacaattg attcacgagc aatttctaaa
    26701 aatatgttat ttataccatt taaaggtgaa aatgttgacg gtcatcgctt tgtctctaaa
    26761 gcattacaag atggtgctgg ggctgctttt tatcaaagag ggacacctat agatgaaaat
    26821 gtaagcgggc ctattatatg ggttgaagac acattaacgg cattacaaca attggcacaa
    26881 gcttacttga gacatgtaaa ccctaaagta attgccgtca cagggtctaa tggtaaaaca
    26941 acgactaaag atatgattga aagtgtattg cataccgaat ttaaagttaa gaaaacgcaa
    27001 ggtaattaca ataatgaaat tggtttacct ttaactattt tggaattaga taatgatact
    27061 gaaatatcaa tattggagat ggggatgtca ggtttccatg aaattgaatt tctgtcaaac
    27121 ctcgctcaac cagatattgc agttataact aatattggtg agtcacatat gcaagattta
    27181 ggttcgcgcg aggggattgc taaagctaaa tctgaaatta caataggtct aaaagataat
    27241 ggtacgttta tatatgatgg cgatgaacca ttattgaaac cacatgttaa agaagttgaa
    27301 aatgcaaaat gtattagtat tggtgttgct actgataatg cattagtttg ttctgttgat
    27361 gatagagata ctacaggtat ttcatttacg attaataata aagaacatta cgatctgcca
    27421 atattaggaa agcataatat gaaaaatgcg acgattgcca ttgcggttgg tcatgaatta
    27481 ggtttgatat ataacacaat ctatcaaaat ttaaaaaatg tcagcttaac tggtatgcgt
    27541 atggaacaac atacattaga aaatgatatt actgtgataa atgatgccta taatgcaagt
    27601 cctacaagta tgagagcagc tattgataca ctgagtactt tgacagggcg tcgcattcta
    27661 attttaggag atgttttaga attaggtgaa aatagcaaag aaatgcatat cggtgtaggt
    27721 aattatttag aagaaaagca tatagatgtg ttgtatacgt ttggtaatga agcgaagtat
    27781 atttatgatt cgggccagca acatgtcgaa aaagcacaac acttcaattc taaagacgat
    27841 atgatagaag ttttaataaa cgatttaaaa gcgcatgacc gtgtattagt taaaggatca
    27901 cgtggtatga aattagaaga agtggtaaat gctttaattt catagagatt agtcgaggga
    27961 ccttttactt ataaaaatga tttgaattaa tactaaaaga ttacaaagaa gaggtggttt
    28021 tgtgtgtaaa tacaaaattg cctttttctt tttatgttaa atctataaat ttgaaactaa
    28081 atcaaggtta attctatgta cacactttat ataggaagta gtttgaatgt ttatataatg
    28141 ttttacaaaa agatgtagta ttataatgtc taatttcaca tgtgtttcag taaaatttgt
    28201 tgtggaatgt taacgatata cgtattttat aaaaaatttt ttataatgat tattcgaatg
    28261 atgcgtaacg cttacatctt atctaatgct agctttttga caaaaatatg acaatcaatt
    28321 aatgtgattc taataaatat tcgcaaattg ctttattgcg attaaatttt tttggtggta
    28381 ctatatagaa gttgatgaaa tattaatgaa cttatatgca aaagtatatt gagaaataaa
    28441 caggtaaaaa ggagaattat tttgcaaaat tttaaagaac tagggatttc ggataatacg
    28501 gttcagtcac ttgaatcaat gggatttaaa gagccgacac ctatccaaaa agacagtatc
    28561 ccttatgcgt tacaaggaat tgatatcctt gggcaagctc aaaccggtac aggtaaaaca
    28621 ggagcattcg gtattccttt aattgagaaa gtagtaggga aacaaggggt tcaatcgttg
    28681 attttagcac ctacaagaga attggcaatg caggtagctg aacaattaag agaatttagc
    28741 cgtggacaag gtgtccaagt tgttactgta ttcggtggta tgcctatcga acgccaaatt
    28801 aaagccttga aaaaaggccc acaaatcgta gtcggaacac ctgggcgtgt tatcgaccat
    28861 ttaaatcgtc gcacattaaa aacggacgga attcatactt tgattttaga tgaagctgat
    28921 gaaatgatga atatgggatt catcgatgat atgagattta ttatggataa aattccagca
    28981 gtacaacgtc aaacaatgtt gttctcagct acaatgccta aagcaatcca agctttagta
    29041 caacaattta tgaaatcacc aaaaatcatt aagacaatga ataatgaaat gtctgatcca
    29101 caaatcgaag aattctatac aattgttaaa gaattagaga aatttgatac atttacaaat
    29161 ttcctagatg ttcatcaacc tgaattagca atcgtattcg gacgtacaaa acgtcgtgtt
    29221 gatgaattaa caagtgcttt gatttctaaa ggatataaag ctgaaggttt acatggtgat
    29281 attacacaag cgaaacgttt agaagtatta aagaaattta aaaatgacca aattaatatt
    29341 ttagtcgcta ctgatgtagc agcaagagga ctagatattt ctggtgtgag tcatgtttat
    29401 aactttgata tacctcaaga tactgaaagc tatacacacc gtattggtcg tacgggtcgt
    29461 gctggtaaag aaggtatcgc tgtaacgttt gttaatccaa tcgaaatgga ttatatcaga
    29521 caaattgaag atgcaaacgg tagaaaaatg agtgcacttc gtccaccaca tcgtaaagaa
    29581 gtacttcaag cacgtgaaga tgacatcaaa gaaaaagttg aaaactggat gtctaaagag
    29641 tcagaatcac gcttgaaacg catttctaca gagttgttaa atgaatataa cgatgttgat
    29701 ttagttgctg cacttttaca agagttagta gaagcaaacg atgaagttga agttcaatta
    29761 acttttgaaa aaccattatc tcgcaaaggc cgtaacggta aaccaagtgg ttctcgtaac
    29821 agaaatagta agcgtggtaa tcctaaattt gacagtaaga gtaaacgttc aaaaggatac
    29881 tcaagtaaga agaaaagtac aaaaaaattc gaccgtaaag agaagagcag cggtggaagc
    29941 agacctatga aaggtcgcac atttgctgac catcaaaaat aatttataga ttaagagctt
    30001 aaagatgtaa tgtcttgagc tcttttttgt tttcaataat tgattctctg tagatatcaa
    30061 agtactaacg ttttaaaggt taaatattta attggattga gatctgtatg cggttatatc
    30121 attctgtgta aatatggttc tccaccaaat gtggtgagta tataatttaa agaactattt
    30181 ttaaattaag aataatcgaa cataaataaa ctttatgaaa tttcagtatc atgttcttat
    30241 aaaaaacaat agggcttttt gctgacgcta gtgcgcgata aataataagt tgaatataaa
    30301 aaagatcact gccaatcatt cgtttaatgg cagcgatctt ttttatttaa ttatttctct
    30361 ttccactgca acatttgata accaatgcgt ggatgtgttt taataatatc ttttgcgtcc
    30421 tcatgacatt gtgaaagttt ttgtcttaat gatgtcatat gcactcttaa actaggcatt
    30481 tcagtcttat taacatagcc atatattcgt tttaatatca tctcataagt gagtactttt
    30541 cctttatgat ttgacaatag ttctaacaag ctaaattcat ttggcgtcaa atgtacctcc
    30601 tgattattaa taacaacaga tttggagcca aagtcgatgc ttagcaaacc gttagtaaat
    30661 acaatgttag tttcttgatg tgacttagcg attctctcga tgactcgtat tcgtgcccga
    30721 agctcatcaa cattaaaagg tttagtcata tagtcattcg caccgttatc taaagcttga
    30781 ataattgttt gttcttcttg tcttgcactt attacaatga taggaatgtc agtatgttgc
    30841 ctgatttctg aaatcaaaca taatccatct ttatctggta aacctaaatc taataaaatg
    30901 acatctggtt tatcaatttg aattttaaag tgtgcttgtg tggcattgtc ggctgtagtt
    30961 acattgtaat aatctaaagt taatgcaaca tcaagtaaat gtgtgattgc gtgatcatct
    31021 tcaattatca atattttaga ttgcattata cgtctccttc gttaaagtct gtatatatat
    31081 tgaaatagaa tatactgccg tgtggttggt tcggtttata ttgtaagttt gattgatgtt
    31141 tgtgtaggat agtctgtact aaatataagc ctagtcccat gctttctttt tggttatctt
    31201 taaaatattt atttgatcct gtgtaaaaag gctcgaatat cttttgttgt tcttctaaac
    31261 taattccagg tccttcgtct ataacggcaa attcgatttg ttcatagcta gcataacgaa
    31321 tagataaatt gattttggtg tcagtagaag tgtgtttaac tgcattttca atcaaattga
    31381 ataaagcttg taaaatcaac ttactgtcaa tgtgtataaa ctgtaaattt actgaggatg
    31441 atacagttat acgctttttt aaatggcgac gttctaaaat catatcgatt tcttctacta
    31501 attcactaac gagataaggt tgcaatttta tctgaacatt tgatgactgt aattttgtta
    31561 atgataaaat atttgtcact aatagatata aatactgact ttcttgaaaa ctatgtacaa
    31621 gtaattgttc cttttctatg atagacatat ctttactatg tgatactaaa atatctaaat
    31681 ttcccataat tgttgttaac ggtgtacgta tgtcatgcga aattgatctt aaaaaatttg
    31741 aatgtgtcag ttgacgttca gcctgtaaca tggattctct cgtttgttta agtaacgtca
    31801 cattttcaac ggcgagagaa agttcattta acattgattc taatattgat gcatcatatg
    31861 gattaatcac ttgagaactt tggtaatcaa tggctagaat gcctttaatc ggagatgtgc
    31921 caattggtat caaccattta ttaatgcctg gaaatgtatc tgttgttgca ccagcttgtc
    31981 tttcattttt aattacccag cttaatgctt gttcatgctg ttgagtcgta ttatcgatat
    32041 ggttttgcaa tggtattgtt ttaattactt tcgattgatt gataacgtat atagtaattg
    32101 attgttgcaa taattgatta atttggtatc cagcatttat tagtaagttt tcaactgtat
    32161 aagtttgttt aatcgaatca ttaaattgaa ataataaatc tgtacgataa agttgctttt
    32221 tagtaatgga gtattggaat ttaatttgtt ttaataaagc actcgttaaa atacttgtta
    32281 aaatgctaac gataaatgta ataggatagt caaagcgata tacttcaaat gtatatctag
    32341 gttccgtaaa aaaataatta aaaacaaata cgttaataat tgctgctaaa aagccaatga
    32401 tgaaggaccg cgtccaaatg gataataaaa tgatgccgat gagaaaaatc attaaaataa
    32461 tcgtactaga ctcatgctta tcaagttgat aaatccacag tcccatcatt acacaaatta
    32521 tttgaatcaa aatcattttt aacatatcta tggcgaaacg tttgccttta ggacgatagg
    32581 gtgatttgtt cattttcaat tcaacaggta tttgtttgat tggaacgatt tcgattttat
    32641 agctatgttc aaaggacatt aaatggtcaa ttaaaggtgt attgaaaaag tctcgccact
    32701 tatttctaat atgttgtccg ataattaatt tggttacatc ttgattttta caccattcgt
    32761 ctaatcctaa tgcaacggtt tggctataaa ctacttttac ttttgctcct aatgattttg
    32821 caagcatgag atgttgatgc acttgctttt gactatcttt atattgcctg tttttttcga
    32881 atacatctat ataaatagca gtgaacttcg catgttcttt ttgagcaata tggaatgcct
    32941 ctttaattac tgcttcatta taaatgctcc cactaattgc cacagcaata tgaggtttga
    33001 gtgacgtttt atggttgtgt cggacttttt ctttatcact catcaagtcg gcaactgttc
    33061 ttaacgtcaa tgtacgcagt tcgcttaggt gggcatacgt aaagaaatta ctaaatgcta
    33121 catctagcct atccttttta tataccttgc cagcttttaa gcgtttaatt aattgttcag
    33181 gtgagatatc tacgacttct aatacatcgg cgctcattat gaaatagtcg ggtacacgtt
    33241 ctttaacatg tacaccggtc atcagttcaa tttgactact taaactttca atatgttgaa
    33301 tgttcaaagt ggtatgaaca tcgataccat gatttaaaat ttcttcaata tccatatatc
    33361 gtttctcatg acgatctcta gaaatattcg tatgtgctaa ctcatcgata agtactattg
    33421 tcggcgattc ttcgattatg cggtcgacat ctaaatattg aaaatgatga ctaccatgtt
    33481 tagtaaaatt ggttgtgatt ttaggtaatt gttcagctaa tgcatttgtt tcatcacgct
    33541 gatgcggttc aatatatcct attttaatat caacgttact ttgaaatagt tcaatggcat
    33601 ttgaaagcat ctcaaatgtt ttaccaacac ctggactgta accgatgtaa attgttaaag
    33661 atccacgctt ttttcctatg tttagcgatt cagtgtttga cataaccttc acctcgatag
    33721 caatatcata atattcttca caattaaatg ctataaaatt tatttctgca tacacatctt
    33781 aatgatttct taataactaa gtttgaaaat taatatttct ctcataaaca agttgttacg
    33841 atgttgttgt gttgtaagga ggttatggca atgctcatcg ttttattact ggtagttatt
    33901 gcattagttt tgtatttatt ttatgcctta attcgtagtg aaaaatttta gaaatatatt
    33961 aagtgagggt tatcatggaa ataattttgt ttctaacaat gatggttatg attacgtatg
    34021 tttttagtgg atatctatat cgcgtagcat tggtgcaatc gtcaagagta gatttaatat
    34081 ttactagatt tgaaaatatg tgttttaaaa ttatcggcac agatttagaa cacatgtcag
    34141 ctaaaacata tgtcaaacat tttctggcat ttaatggatt tatggggttt ataacatttg
    34201 tattgctaat agttcagcaa tggctttttt taaatccaaa tcataattta aaccaatcga
    34261 tagatttagc gtttaataca gcaatatctt ttttaacaaa tagtaattta caacactata
    34321 acggtgaatc agatgtgaca tatttaacgc aaatgattgt aatgacatat ttaatgttta
    34381 catctagtgc atcaggttac gccgtttgta tagcgatgtt gagacgttta actggattaa
    34441 ctaatatcat tggtaatttt tatcaagata ttgttcggtt tattgtccga gtacttttac
    34501 cattatcatg tttaattagt attttattga tgactcaagg tgtaccacaa acgttgcatg
    34561 ctaatttaat gattcggact ttaagcggac atattcaaca tattgcattt ggacctattg
    34621 catcacttga atcaataaaa catcttggta cgaatggtgg aggattttta gcaggaaatt
    34681 ctgcgacacc ttttgaaaat ccaaatattt ggagcaattt tatagaaatg ggcagtatga
    34741 tgttacttcc tatgtcaatg ttgttcttat ttggtcgcat gttaagtaga catggtaaac
    34801 gagtacatcg tcatgcgttg atattatttg tcgcaatgtt tttcattttt atagcaattc
    34861 ttacattaac tatgtggagt gagtatcgtg gtaatccaat actagcgaat ttaggcattt
    34921 atggaccgaa tatggaaggt aaagaggtac ggtttggagc aggtttgtca gcactattta
    34981 cagttattac gacggcattt acaacgggtt ctgttaataa catgcatgat agcttaacgc
    35041 ctataggtgg attaggacca atggtattaa tgatgctaaa tgttgtattt ggtggcgaag
    35101 gcgtaggact catgaattta ttgatatttg tcttactgac ggtgtttata tgcagtttga
    35161 tggttggtaa aacaccagaa tatttaaata tgccaattgg cgcccgtgaa atgaaatgta
    35221 ttgtcttagt ctttctcata cacccaattt taattttagt attttcagca cttgctttta
    35281 tgattcctgg agcaagtgaa agtataacga atccgtcttt tcatggtatt tcacaagtta
    35341 tgtatgaaat gacatcagct gctgcgaaca atggatcagg gtttgaagga ctgaaagatg
    35401 atacaacatt ctggaatatc tctacaggaa tcattatgtt gctttctcgt tatataccaa
    35461 ttattttgca attaatgatt gcatcaagtt tagtgaataa aaaatcatac catcaagata
    35521 aatatacaat agcgattgat aaaccttatt ttggcgtatc actaatcgta tttatcgttt
    35581 tactaagtgg tttgacattt attccagtac tattacttgg tcctattggt gaatttttaa
    35641 ctttaaaata aagtaaaggg atggaaaaaa tgcatcatgt aaataaatat ttcaatcaaa
    35701 caatggtgat agaagctttg aaaatgagtt tttataagct gaatcttaaa caattaataa
    35761 aaaatccgat aatgtttgtc gttgaggttg gaatgatttt aacgttgata ttaatatgtt
    35821 ttcctgacat atttggaact agctatctat cgcgaggtta tttgattaca atttttatca
    35881 tattattaat aactatattg tttgctaatt tttcagaagc ctttgctgaa ggtcgtggaa
    35941 aagcgcaagc tgatagttta agacaagcac aatcaaattt aactgctcga ttgatagaag
    36001 aaaatggtgc gtatcggata gtgaacgcca cagagttaaa agccggacaa aatattcggg
    36061 tagaaaatgg agaaacaatc cctgctgatg gcgttgtaat aaatggatta gcaaccgttg
    36121 atgaatctgc aattactggt gagtctgcac ctgtgataaa agaatcaggt ggcgattttg
    36181 atggagtcat tggcggtaca cttgtaacat ctgattggtt agaaatacgt gttgagagtg
    36241 aagcgggtac atctttttta gataaaatga ttgccttagt tgaaggggcg gaaagaaata
    36301 aaacaccaaa tgaaattgca ctttttacat tattaacaac gttaacaatt atatttttag
    36361 tcgttattgt aacgttatat ccaatagcat cttacctgca tttaatttta cctattgcga
    36421 tgctcattgc gttaacagta tgtttaatac caacgacaat cggtggtttg ttatcggcga
    36481 ttggtattgc aggcatggat cgtgttactc aattcaatgt attagcgaaa agtgggcgtg
    36541 ctgtagaagt atgtggagat gtcgatgtaa tgattttaga caagacaggc acaatcactt
    36601 atggcaatcg aatcgctagt gaatttttac cagtgaatca acaaatgctt gaaaaattga
    36661 tagtcgctgc ttatatgtca tcaatctacg atgatacgcc agaaggaaaa agtattgtac
    36721 gattagcgaa gcagatgtat ataaatgagc tgcctaaaga cattgatggc acatataagc
    36781 cttttacagc tgaaactaga atgagcggaa tcattacaaa tgaaatatcg gtattcaaag
    36841 gtgcacctaa tagtatgata aatcttgtca aacaacaaca aggcaatatt cctcttaata
    36901 ttgagtctct ctgtatggat gtatcgagta aaggtggcac accattaata gtcattgaaa
    36961 acaatgtcat gcttggcgtg atttatttaa aagatgtgat taaagatggt cttgtagaac
    37021 gatttactga attgagaaaa atggggattg aaacagttat gtgtacaggt gataatgctt
    37081 taacagcagc tacaattgca aaagaagccg gggttgatag atttgttgca gagtgtaaac
    37141 ccgaagataa aattaaagtc atcaaagatg aacaagcgaa aggccatatt gttgcgatga
    37201 caggagatgg tacaaatgat gcacctgcgc tagcacaagc gaatatagga ttagctatga
    37261 actcagggac aattagtgct aaagaggcag ctaatctaat tgatttagat tcgaatccta
    37321 caaagttaat agaggttgta aaaataggca aacaattatt aatgacgaga ggtgcattaa
    37381 cgacatttag tttggctaat gacgtagcaa aatactttgc aattttacct gcactaatga
    37441 tgtcaacaat tccagaaatg acttcactaa atattatgca tttatcgtca cctaaatctg
    37501 caattatatc agctttgata ttcaatgcat taatcattgt tgccttaatt ccaattgcca
    37561 tgaaaggtgt aaaagtaaag ggttacagca ttgatagaat ctttataaat aatatgttga
    37621 tttatggatt aggtggatta atcgtcccgt ttttaggcat taaattaata gatatgattg
    37681 tacaattttt tgtgtaaaga ggaaggagcc tgtaccatga atacgataag aaatagtatt
    37741 tgtttaacga taataactat ggtattatgt ggatttttat tcccgttagc tatcacgtta
    37801 attggtcaaa tattttttta tcaacaagca aatggcagtt taattacgta tgacaatcgt
    37861 atagttggat caaaattgat tggacagcac tggacagaga cacgatattt tcacgggaga
    37921 ccgagtgcag ttgattataa tatgaatcct gaaaaattat ataaaaacgg cgtatcttca
    37981 ggtggaagta atgaatcgaa tggaaacacc gaattaatag cacgcatgaa acatcatgtt
    38041 aaatttggga attcaaatgt aacaatagat gctgcaacaa gttctgggtc gggcttagat
    38101 ccgcatatta cggtcgaaaa tgcattgaag caagcacctc gtattgctga tgctcgtcat
    38161 gtatctactt caagagtggc cgacttgatt caacatcgta agcagcgtgg tgtgttaaca
    38221 aatgattatg tgaatgtgct tgaactaaat atcgctcttg ataagatgaa agattgatta
    38281 tagatgaaag gcatcatgtt gataaaatta tacatggtgt ctttttagca attcaagcac
    38341 tttctgctta attaaatttt tagatgtacc tttaggaaga ataaattagc atgaaatatt
    38401 ccatatgcta taattatcat catataaaaa taaaatgaaa tagattataa atccgtataa
    38461 ttttttattt ggggagaggg gtatatgtca gattatacta agttgactaa gagtcctaag
    38521 tcagcattgt tgtattacta cattacgaat ggcttagaat ttatattgag tgtagctgtg
    38581 tatgtcattt tttattttat atggttaaga tttgaatggc cacattactt actttatatt
    38641 ttgttggttt taggtgctct gagtacatta aaattattta ttaagccttt atggcaatat
    38701 cattgtcgct tttataaagt tgatcaacta agtgtacaat atcgtacatc attttttata
    38761 tataaagaag aaacgagtcg aattgaacgt ttacaatact tgtcgattaa atctaatcca
    38821 attagtaaag tgcttaattt gtataaagtt ggatttatga ctgcaggtca tactatttat
    38881 ttaccgatga tgtctcatga cgatgttaaa ataatcgaag agagaactat gtcgaatttg
    38941 agaggggttg aaagtgatgt ctgaacctca aaaattacat ccgatttcat attttaatgg
    39001 cataatcaat gctatcaaac aaaacatagt cgtctttttt attttcatta tctttcaatt
    39061 gaaagatttt gattatacga atcccgaatc atatttatgg ataggtattg tatttgtatt
    39121 ctttttaata tcatacattt ctcaaattgt cggaattatg aacacgcgat attggattga
    39181 ggataattat ttcattttaa caacaggtat ttttaataaa aagcgtaaag aattaaatat
    39241 aaaacgtatt cagtccgttg atatgacaca aggggtcgtc aatcaaatta ttggtggtgt
    39301 ggatttacaa atcaaaacac caagtgatgg tattgtctta agtgttattt cgaaaaaaca
    39361 aggtgaatat cttgaacgat atattgatca attgcaaaca gagttgaaga cagaagtggc
    39421 gcaaaagtct agtgctgaga agaatgacaa ctgtaatcat gacattgaag gtaatgtgga
    39481 agacatcgca gaaggtcgct tagatgaagt gcagaacact gatagtatgc ttaatcagaa
    39541 aaataaacgc attcctatat atcagatgaa ttttaaagaa tcactattta tggcaatgac
    39601 cagtggcgcc attggtgtaa cgttagcagc tttagtaccg atttacgggg ctgtaagaga
    39661 gttgatccca tggtcgaagc taaatcatga aattagtcaa tgggttcaaa ttgtaagtat
    39721 ttcagtatta atcatcattt tactagtatt aatagtggca tatattattg gaacgattat
    39781 aacaatgata agattttatg gcttttcggt gatgcttgaa aatgagcaac ttaaaattga
    39841 atatggtctc tttaacaaga agaaagtaac tgtaccaacg aaacgattgc aagctgttgt
    39901 tgaacgtcag tcatatatac gaagattatt tggctacaca gcaatacatt ttatcatcac
    39961 aagtgatttt gaaaataaaa aaatcgaaga tgatgacaat gattcaggta gtgtgccgat
    40021 attgccgttt attaaacgag acaaagcata tcaaattatc cagaatttag taccagattt
    40081 atcttatcaa caagttaatg aaggtatgcc tttgtcaggt ttccacagac actttttaat
    40141 acctagtatt attgtcatag ctatggcagg tataggctgt tatttttgga atcaattatt
    40201 attgttattt acgattataa caatagtcat tattggttta tttgtgatca aaggatatct
    40261 ttatgtcaaa catgcgggtt ttaaaatgac agaagaacaa attactatcc agaatttcga
    40321 gctattgaaa cgaaatattt attattttaa agcgcatcac attataggat ttgacacttg
    40381 gcaacataca ttacttaaac gcaacaattt aaataatttt agctttattg ttgctaaagg
    40441 tgtgggagcc caatcgatta acttaaaata cgcaagaagt gaagatgcaa ataaattgaa
    40501 atcatggtat ttaagaggtg aaaatgatga gggattataa ttttatgtca ccacacgcta
    40561 aaaaagtgat gaggttaagt gctgtgctgt tttggatacc catagcgctt gtacttttaa
    40621 tcgcatttaa tattttgaac tggctatttt gggagatttt agataaccat ttatccattg
    40681 ttagtagtgt gattataata ttacttgttg ctgttttcac gcttgttata gcgccagtct
    40741 atcgatataa gcactgcaga tatgcatttg aaaaacacca tttgcgtgta cgaaacggca
    40801 ttttattttt ggatgaaaag gtaataccat attttcgtat tcaaaatatt gatatcgatg
    40861 ttggtcctat aatgagacgc tatcgattag caactttgac cctttataca gcgggtggtc
    40921 aagctgaaat tgaattaata gatagaagtg aagcacgtag attgaaacga tggttaaata
    40981 acgagagaca tttagaaaac aataaaaatg aggaataatc aatgatacat ggaattggtg
    41041 tagatttaat cgaaatcgat cgaatacaag cgttatatag taagcaacca aaattggttg
    41101 agcggatttt aactaaaaat gaacagcaca aattcaacaa tttcacacat gagcaacgta
    41161 aaattgaatt tttagctggc aggtttgcta caaaagaagc gttcagtaaa gcattaggca
    41221 caggcttagg aaaacatgta gcttttaacg atatagactg ttacaacgac gaacttggca
    41281 aaccaaagat tgattacgaa gggtttatcg tacatgttag tatctcacac actgagcatt
    41341 atgcgatgag ccaagttgtt ttagaaaagt cagcatttta aatttgttat acaatgataa
    41401 ttaagatcta gttttaaaac gtgtgggaat tacaggagga agtaatatgt cggataaata
    41461 ttatagatct gcgtatatga atgtagattt aaacgctgtt gcatcaaatt tcaaagtatt
    41521 cagtacattg catccaaata aaacagtgat ggctgtcgtt aaagccaatg cctatggact
    41581 aggtagtgtt aaagtagcac gtcatttaat ggaaaatggc gccacatttt ttgctgtagc
    41641 aacgttagat gaagcgatag aacttagaat gcatgggatt actgctaaaa ttttagtctt
    41701 aggtgtgtta ccagctaaag atattgataa agcgatacaa caccgagttg ccttaacggt
    41761 tccgtctaaa cagtggttga aagaagcaat taaaaacatt tctggtgagc aagagaaaaa
    41821 gttatggttg cacattaaat tagatacagg aatgggacgt ttaggtatta aagatactaa
    41881 aacgtatcaa gaagtgattg aaatcattca acaatatgag caacttgtat ttgaaggcgt
    41941 gtttacacac tttgcctgtg ctgacgaacc aggagatatg acaactgaac aatatcaacg
    42001 ttttaaagat atggtcaatg aagcaattaa acctgaatat atacattgtc agaactcagc
    42061 aggctctcta ttaatggatt gccaattctg taatgcaata agaccaggaa tttcccttta
    42121 tggatattat ccatcagagt atgtacagca aaaagttaaa gtacacctta aaccaagtgt
    42181 gcaattaatt gctaatgtag ttcaaacaaa gacgctacaa gcgggtgagt ctgtaagtta
    42241 tggtgcaact tatacagcta ctgacccaac tacaatagca ttgttaccta ttggatatgc
    42301 agatggctat ttacgcataa tgcaaggtag ctttgtaaat gtaaatggtc atcaatgcga
    42361 agttattggt cgcgtatgta tggatcagac aattgttaaa gtgccagatc aagttaaagc
    42421 tggagattcg gtgattttaa tagataatca tagagaaagt ccacagtcgg tagaggtggt
    42481 agctgaaaag caacatacta ttaattatga agtgctttgt aacttgtcga gacgtttgcc
    42541 gcgaatctat catgatggtg atcaacgttt tgtaacaaat gaattgttaa aataatatgg
    42601 tcagtgcata taaaatttgt tatcattaga gtaattaaag gtcatttaat aacttttgga
    42661 atcaattgga ggttctcata tgttatcttt tagtcaaaat agaagtcata gcttagaaca
    42721 atctttaaaa gaaggatatt cacaaatggc tgatttaaat ctctccctag cgaacgaagc
    42781 ttttccgata gagtgtgaag catgcgattg caacgaaaca tatttatctt ctaattcaac
    42841 gaatgaatga ttagacgagg agatgtttat ttagcagatt tatcaccagt acagggatct
    42901 gaacaagggg gagtcagacc tgtagtcata attcaaaatg atactggtaa taaatatagt
    42961 cctacagtta ttgttgcggc aataactggt aggattaata aagcgaaaat accgacacat
    43021 gtagagattg aaaagaaaaa gtataagttg gataaagact cagttatatt attagaacaa
    43081 attcgtacac ttgataaaaa acgattgaaa gaaaaactga cgtacttatc cgatgataaa
    43141 atgaaagaag tagataatgc actaatgatt agtttagggc tgaatgcagt agctcaccag
    43201 aaaaattagg cgtctattat atgtattttt cagagataaa taaaatattg atataaaaga
    43261 caataacttt ataataatta taactatttc taaattctgt acgaagaatt ttcttataaa
    43321 caaagatttt agcaaatacc agttatgata ttcatatttt ttattataaa aggatgtctt
    43381 aagtttttta ggctttaggt attccatcct aaagtttttt tagcttaaaa gtatcatcta
    43441 cagcaaaatt gcaaacgaca aaattgataa gtgcaattaa ataaatgtta gtaagtgaat
    43501 cataattatc cttgcttaag catttgcttt gtaagggaag tgaggaggca actaatcgtg
    43561 gaagaattta agcaacatta taagggttta attgatgaaa gtttaacgtg ccaagataaa
    43621 gtagaattga taaaaaagtg tgagaaatac actgacgaag tgattcgtaa ggacgtcttg
    43681 cctgaagaca ttgtcgatat tcacaaaaac tatatattga cgttaaactt aacgcgtgaa
    43741 gatgtgttca agacattaga tgtcttacaa gaaatcgtta aaggctttgg ttatagttat
    43801 cgagattatc aaagattggt agataaactt caagttcacg ataaagagat agacttagct
    43861 tctagcttac aacaaacaat gcttaaaaca gatattccac aatttgatag tattcaaatt
    43921 ggcgttattt cagtggcggc acaaaaagta agtggagatt attttaattt aattgaccat
    43981 aacgatggca caatgagctt tgctgttgca gatgtcattg gaaaaggtat accagctgct
    44041 ttagcaatga gtatgataaa gtttggcatg gattcttatg gacactcaca attaccgagt
    44101 gatggtttaa aacgtttaaa tcgtgttgtt gaaaagaata ttaatcaaaa tatgttcgtc
    44161 acaatgtttt atggtttata tgaagaaatg aaccatttat tgtattgtag ttcagctggt
    44221 catgagcctg gatatattta tcgcgctgaa aaagaagaat ttgaagaaat ttcagttaga
    44281 ggtagagtgt taggaatcag ttcacaaaca cgatatcaac aacaagaaat tccaatatac
    44341 cttgatgatt taattatcat tttaacggat ggtgtgactg aagctagaaa tagtgaaggt
    44401 acctttatag ataaacaaaa acttttagaa tatattaaaa aacataaaca tatgcaccca
    44461 caagatattg ttcaaattat ctatgaagca attttaaagc ttcaaaaccc aaataaaaaa
    44521 gatgatatga ctattttgat tataaaaaga gtaaattaat ttaaaaaaga agattagaaa
    44581 ttatttcgat gggtatataa taatttgaaa tataaatatg gtggatacag cgcttaaaat
    44641 gaagataaat atttttaata agtaggagtg taatgaaatg aatcttaata tagaaacaac
    44701 cactcaagat aaattttacg aagttaaagt cggtggagaa ttagatgttt atactgtgcc
    44761 tgaattagaa gaggttttaa cacctatgag acaagatgga actcgtgata tttatgttaa
    44821 tttagaaaat gtgagttata tggattcgac aggtttaggt ttattcgtag gtacattaaa
    44881 agcattaaac caaaatgata aagaactata cattttaggt gtgtcagatc gtatcggtag
    44941 actatttgaa attactggtc ttaaggattt aatgcatgtt aatgaaggaa cggaggtcga
    45001 ataacatgca atctaaagaa gattttatcg aaatgcgcgt gccagcatcg gcagagtatg
    45061 taagtttaat tcgtttaaca ctttctggcg ttttttcgag agctggtgct acatatgatg
    45121 atattgaaga tgccaagatt gcagttagtg aagctgtgac aaatgcagtt aaacatgcat
    45181 acaaagaaaa taacaatgtg ggcattatta acatatattt tgaaatttta gaagataaaa
    45241 ttaaaattgt tatttctgat aaaggtgaca gttttgatta tgaaacaact aaatcaaaaa
    45301 taggtcctta cgataaagac gaaaatatag actttttacg cgaaggtggc ctaggtttat
    45361 ttttaatcga atctttaatg gatgaagtca cagtatataa agaatctggt gtgacaatca
    45421 gtatgactaa gtatataaaa aaagagcagg tgcgaaataa tggcgaaaga gtcgaaatca
    45481 gctaatgaaa tttcacctga gcaaattaac caatggatta aagaacacca agaaaataag
    45541 aatacagatg cacaggataa gttagttaaa cattaccaaa aactaattga gtcattggca
    45601 tataaatatt ctaaaggaca atcacatcac gaagatttag ttcaagttgg tatggttggt
    45661 ttaataggtg ccataaatag attcgatatg tcctttgaac ggaagtttga agccttttta
    45721 gtacctactg taatcggtga aatcaaaaga tatctacgag ataaaacttg gagtgtacat
    45781 gttccgagac gtattaaaga aattgggcca agaatcaaaa aagtgagcga tgaactaacc
    45841 gctgaattag agcgttcacc ttctatcagt gaaatagctg atcgattaga agtctcagaa
    45901 gaagaagtgt tagaagcaat ggaaatggga caaagttata atgcgttaag tgttgatcat
    45961 tccattgaag ctgataaaga tggttcaact gttacgctat tagatattat ggggcaacaa
    46021 gatgaccatt atgacttaac tgaaaaacgt atgattttag aaaaaatatt acctatatta
    46081 tctgatcgcg aacgagaaat catacaatgt acgtttattg aaggattgag tcaaaaagag
    46141 acaggtgagc gtatcggttt aagtcaaatg catgtatcac gacttcagag aacggcaatt
    46201 aagaaattac aagaagcagc acatcaatag aatttgttta ttaatgatac gttttataat
    46261 gaaaaatcca tataattatc ccttgattat taaattgaaa tcgaggggta tttttaattt
    46321 aattaagatt ttcgaattaa tacattatta gtgtagttta atgtgtatcc acataaatgt
    46381 cgcgatatag tattaataat ttaagtgaag aagatatcta attgtcgttt taaataggtg
    46441 ggttgctatt agaataaaaa aagtagtctt agattatgaa atttagaaat gatggtgtgt
    46501 cattttcaat aatcttagtg cgttttaaaa tatagtatga cctaatcatt cgttttaaat
    46561 gttttggaag tgaaaattac attaagtatc ataccttaat agaagtattt tagaatatgt
    46621 taaaataaat gagtaaattt aagaaaaagt gtgggttaag taaatggaca atcaattgat
    46681 taattcaatc atagagaaat atcaatttag taaaaaacaa attgaagcag tattaacact
    46741 gctagaagaa aaaaatacag taccatttat tgcgaggtat cgaaaagagc aaactggtgg
    46801 actagatgaa gttcaaataa agcaaattga tgacgaatac caatatatgg tcaatttaca
    46861 aaaacgtaaa gaagaagtta tcaaaaatat agaacagcaa ggattactta ctgaggaatt
    46921 aaagaaggat attttaaaac agaacaaatt acaacgtgtt gaagacctat ataggccttt
    46981 taaacaaaag aaaaagacaa gggcaactga ggcgaaacgt aaagggttag agccattagc
    47041 gatatggatg aaggcacgta aacatgaagt ctcaattgaa gaaaaagcac aacaatttat
    47101 aaatgaagaa gtgcaatcgg ttgaagatgc tatcaaaggt gcacaagata ttattgcgga
    47161 acaaatttca gataatccta aatatagaac aaaaatttta aaagatatgt atcatcaagg
    47221 tgtgttaact acatctaaaa agaaaaatgc tgaagatgaa aaaggtattt ttgaaatgta
    47281 ctatgcatat agtgagccaa ttaaacgcat tgctaatcat agagttttag ctgttaatcg
    47341 tggtgaaaaa gagaaagtat tatctgtaaa gtttgaattc gatacgacat cagtagagga
    47401 tttcattgca cgtcaagaaa tcaatcataa taatgtaaat cgcagttata ttttagaggc
    47461 gattaaagat agcttgaaac gcttaattgt cccttcgata gagcgtgaaa tccatgctga
    47521 tttaactgaa aaagctgaaa atcatgcaat agatgttttt agtgaaaact taagaaatct
    47581 attactgcaa cctccaatga aaggtaaaca aatattaggc gtagatccag catttagaac
    47641 aggttgtaaa ttagcagtca ttaacccatt cggtactttt atagcaaaag gtgtgattta
    47701 tccgcatcca ccagtttcta aaaaagaggc agcagagaag gattttgtac aaatggttaa
    47761 agcgtatgat gtgcaattaa ttgcaattgg caatggtact gcaagtcgtg aaacagaaca
    47821 atttgttgca gatttaatta aaaagcatca gttgccagta caattcatca ttgtcaatga
    47881 agcgggcgct tcagtatact cagcatcaga aattgctaga gatgaatttc ctgattttca
    47941 agtggaagaa agaagtgccg tatcaattgg acgacgcgtt caagatccat taagtgaatt
    48001 agttaaaatc gatcctaaat ctattggtgt cggacaatac cagcatgatg ttaatcaaaa
    48061 agcactagaa aatgcattaa ctttcgttgt tgaaacagca gttaaccaag taggtgttga
    48121 tgtcaataca gcatcatcat cattattaca atatgtgtct ggtctaagtt cgcaaattgc
    48181 taaaaatata attgcatata gagaagaaaa tggcgcaata aagcataata aagagttaag
    48241 caagattaag cgtttgggtg ctaaaacttt tgagcagagt ataggatttt taagaatagt
    48301 agacggttca gagccattgg ataatacatc gattcatccc gaaagctata aagtaacgta
    48361 tcagttatta gataaactcg gatttggtgg caatgattta ggtagtgatg cattaaaagc
    48421 taaattaaat tcattggata tggatgaact ggcgattgaa ttacaagtcg gtgtacctac
    48481 attagaggat attattaaat ccttgaaagc acctaatcga gatccaaggg acgagtttga
    48541 aacaccaatc cttaaatccg atgtgctatc aattgaagat ttacaagaag gaatgaaatt
    48601 gagtggtaca gttagaaatg tagtagattt tggagcattt gtagatatag gtgttaaaca
    48661 agatggactt gtccatgttt cgaaactttc taagaagttc gtaaaaaatc caatggatat
    48721 tgtcagtgtt ggtgatatcg ttgacgtttg gatttacagt attgacaaaa ataaagacaa
    48781 agtatcatta acgatgattg atccacatga ataatgacaa acttcagcga atggttgaga
    48841 atctttcgga agaaaaattt ggacgtacgt ttcgacattg tgcatatttt aataaaaggt
    48901 tacgtacaac aggcggacgt tatcttttaa agtcccatga tattgaaatt aatccaaaac
    48961 aatatgagca ttatggagaa gatgctgttg taaaaattat actgcacgaa ttgtgtcatt
    49021 atcacttgca tattgctggt aaaggttatc aacataaaga tcaagatttt aaacgattga
    49081 gccaacaggt tggagcgcct agattttgta acagcattga aagctatcaa caacgggcaa
    49141 attatgaata ttattgcact aaatgtcatg caaaatatat acgtatccgt aaagttgata
    49201 cgaatcgtat gcgttgcgga cattgcaatg gaaaattaag aatgaaaaga caattgaaat
    49261 aagtacaata caaatagagc taattcgtgt atatgtaaga ttatttatga gttgtatgga
    49321 atttatatag aatttatcag tgagttattt tgattggtaa acatagtaat tgaataagaa
    49381 gaaattgtgt ttataaaatg tttaaagccg agattgtcgt aatttaatac ttcgcaatct
    49441 cggcttttct acttatgata taggtaatgg cataaattgt ggcttgatag gatgaaatta
    49501 aaatgattag attaaaaatc ttggcgtatt ttagtaa
//
LOCUS       DAKTIP010000003        45498 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAKTIP010000003 DAKTIP010000000
VERSION     DAKTIP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 45498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.003"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            134..895
                     /gene="sufC"
                     /locus_tag="O0305_000100"
     CDS             134..895
                     /gene="sufC"
                     /locus_tag="O0305_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCY0273287.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            993..2300
                     /gene="sufD"
                     /locus_tag="O0305_000101"
     CDS             993..2300
                     /gene="sufD"
                     /locus_tag="O0305_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCY0273288.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2406..3656
                     /locus_tag="O0305_000102"
     CDS             2406..3656
                     /locus_tag="O0305_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0273289.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDVDQLINALKQTKEFFSYEF"
     gene            3646..4110
                     /locus_tag="O0305_000103"
     CDS             3646..4110
                     /locus_tag="O0305_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCY0273290.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4261..5658
                     /gene="sufB"
                     /locus_tag="O0305_000104"
     CDS             4261..5658
                     /gene="sufB"
                     /locus_tag="O0305_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCY0273291.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            complement(5726..6775)
                     /locus_tag="O0305_000105"
     CDS             complement(5726..6775)
                     /locus_tag="O0305_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0273292.1"
                     /translation="MPVYKDDNTGKWYFSIRYKDVYGNNKRKMQRGFSTKREAKRAEA
                     IFLNDVNEGYSDSKTFDYVFHHYLENSDLRPKTKRRKQNEYHKHFKAKFGHIKMNKIT
                     QNQCQEFRKYLIENVASTNSARTIWSGFKVVINYAKKYFGLRTDPTISIKPIPRVKPK
                     PKFMMREEFEERIKDIEEQDYRELFTLMFYTGLRIGEAMALVWTDYNKYKKEISINKT
                     MDISNRTIYPRPKTDSSEDIVPLPKFINTMLTERHQREKELNKYFDERSYFIFGGMAP
                     KHYSHVQKKFQKAFPHYNIHALRHSYASYLANNGVDIFVLQSLMRHAQITETMGTYSH
                     LYTQKKHDAIAIFDK"
     gene            complement(6838..7353)
                     /locus_tag="O0305_000106"
     CDS             complement(6838..7353)
                     /locus_tag="O0305_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273293.1"
                     /translation="MKRLLYLILASALVLGACGSNDGDKKEESKKAETKKENKDKKKE
                     TKDKAEVKKENANQNDNNNQVNNDNNTNVNDHQQTNNAPKQNQTQNNPTSNKNNNAPV
                     KDEFSSDTSYNAYQEAKRATEENKRQNGGHTAGIGGSWAVQDGQDYNSWKKAQNDFDN
                     FKRQNSEVIQQ"
     gene            complement(7357..7857)
                     /locus_tag="O0305_000107"
     CDS             complement(7357..7857)
                     /locus_tag="O0305_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273294.1"
                     /translation="MAGDKLTFKEILTETKMFSKLSNRKIDMYKKMTTDEKRKILNDF
                     KEGKELDIQLYKSENFKNTNEEYESKSAKSLNGQGIKEATDVTTYAYQKQNINPTLLK
                     VYNGLGTFTTNVDKQAKFVFYDTQLKQNFVSIAQRDELIKQNNRIIEQNNEVIDLLKQ
                     IANKGV"
     gene            complement(7908..8546)
                     /locus_tag="O0305_000108"
     CDS             complement(7908..8546)
                     /locus_tag="O0305_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCY0273295.1"
                     /translation="MAFKNSIKEIRLNNRLSKVEMARKLDVSEGTIRMWESGRTEPRM
                     GMVEKISSLFNVSKGYLLGEIEEIVLPEFDSEIEVPYFGKVSAGNFEEVAIENEKLKV
                     PPFAFNGRKPSECIALKINGDSMNKILANGSYIIVHDYRKSCDHKLNSNDILVLRLGG
                     EYTVKRVRRTETKLHLDPVSYSDEFKTNSYDLDSIDEIEVIGKVIYNYRIFD"
     gene            8737..8991
                     /locus_tag="O0305_000109"
     CDS             8737..8991
                     /locus_tag="O0305_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273296.1"
                     /translation="MNYIKQSLKLDEWRKRKGYTQSSFAEKLGISPSTYNIWENNPEM
                     IKPRDAFRIAKTLNISIDEIIFLKDESYFKYVLVEGKQMS"
     gene            9007..9798
                     /locus_tag="O0305_000110"
     CDS             9007..9798
                     /locus_tag="O0305_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0273297.1"
                     /translation="MQALQTFNFKELPVRTVEIENEPYFVGKDIAEILGYARTDNAIR
                     NHVDSEDKLTHQFSASGQNRNMIIINESGLYSLIFDASKQSKNENIRETARKFKRWVT
                     SDVLPAIRKHGIYATDNVIEQTLKDPDYIITVLTEYKKEKEQNLLLQQEIGELKPKAD
                     YVDEILKSTGTLATTQIAADYGISAQKLNKLLHEARLQRKVNKQWVLYSEHMGKSYTD
                     SDTITIVRSDGREDTVLQTRWTQKGRLKIHEIMTEFGYEANLGGA"
     gene            9799..10023
                     /locus_tag="O0305_000111"
     CDS             9799..10023
                     /locus_tag="O0305_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273298.1"
                     /translation="MTPEQKEKLNNIVLTLYAVKENKSQTYTHKDTLTVTYAGEIEHT
                     YEVDREKHLESMIEWAIDQIEQHFDLDEEE"
     gene            10063..10512
                     /locus_tag="O0305_000112"
     CDS             10063..10512
                     /locus_tag="O0305_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273299.1"
                     /translation="MNALYKTTLLTTMAVVTWKVWKIERNTRKPVINRNDFSKESTAE
                     TIERHSDPDSGIKLLKAFSDFTKQAEKQKPTLGEVYRRNKPELPTVTLDENGLFINDF
                     RVPYVLEEGVNVKKSMNNLYKVSLDFFAKSIIADNYEADNPENQQLF"
     gene            10526..10747
                     /locus_tag="O0305_000113"
     CDS             10526..10747
                     /locus_tag="O0305_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273300.1"
                     /translation="MMKNSLQAKELAVILSVSKSKAGQIIRELNKELEDEGYIAIRGR
                     IPVQLARKKFPYHDLSDERIMEELKKENE"
     gene            10740..10907
                     /locus_tag="O0305_000114"
     CDS             10740..10907
                     /locus_tag="O0305_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0273301.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIGTFI
                     FYKEYFYGVDD"
     gene            10908..11228
                     /locus_tag="O0305_000115"
     CDS             10908..11228
                     /locus_tag="O0305_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273302.1"
                     /translation="MTWFEEYVKPSVEWERKAEQAVLSDDEVKTITEYRKKYNNPHIY
                     MSAQNRNYLVEYLDRHTGDIVLHNLKLKKSSRRRVHQYLMVGQIVVPGEPKGTIYEAS
                     LIIR"
     gene            11322..11582
                     /locus_tag="O0305_000116"
     CDS             11322..11582
                     /locus_tag="O0305_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0273303.1"
                     /translation="MYYEIGDVCQKVINVDGFDFKLAVKKKDHSILVNILDLEDKFID
                     GINITNENDLYTALDILNQSIYEWIEENADDYDRLINLVMKW"
     gene            11592..11813
                     /locus_tag="O0305_000117"
     CDS             11592..11813
                     /locus_tag="O0305_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2483 family protein"
                     /protein_id="HCY0273304.1"
                     /translation="MKQTVTYIIRHRDMPIYITNKPTDNNSDISYSTNRNRAREFNGM
                     EEASINMDYHKAIKKTVTETIEYEEVEHD"
     gene            11806..12585
                     /locus_tag="O0305_000118"
     CDS             11806..12585
                     /locus_tag="O0305_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0273305.1"
                     /translation="MTEQTNQDVDILTQLDVKDISKQNANKFYKFAIYGKFGTGKTTF
                     LTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVVKMLPKIIEQLRENGKQIDVV
                     VIETIQKLRDITMDDIMDGKLKKPTFNEWGECASRIVSIYRYISKLQEHYQFHLAISG
                     HEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKAYQYVL
                     NAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN"
     gene            12616..13167
                     /locus_tag="O0305_000119"
     CDS             12616..13167
                     /locus_tag="O0305_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0273306.1"
                     /translation="MKITGQAQFTKETNQEKFYNGSAGFQAGEFTVKVKNIEFNDREN
                     RYFTIVFENDEGKQYKHNQFVPPYKYDFQEKQLIELVTRLGIKLNLPSLDFDTNDLIG
                     KFCHLVLKWKFNEDEGKYFTDFSFIKPYKKGDDVVNKPIPKTDKQKAEENNGAQQQTS
                     MSQQSNPFGSSGQFGYDDQDLAF"
     gene            13180..13845
                     /locus_tag="O0305_000120"
     CDS             13180..13845
                     /locus_tag="O0305_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273307.1"
                     /translation="MQYITRYQKDNDGTYSVVATGVELEQSHIDLLENGYPLKAEVEI
                     PDNKKLSIEQRKKIFAMCRDIELHWGEPVESTRKLLQTELEIMKGYEEISLRDCSMKV
                     ARELIELIIAFMFHHQIPMSVETSKLLSEDKALLYWATINRNCVICGKPHADLAHYEA
                     VGRGMNRNKMNHYDKHVLALCREHHNEQHAIGVKSFDDKYHLHDSWLKVDERLNKMLK
                     GRE"
     gene            13845..14627
                     /locus_tag="O0305_000121"
     CDS             13845..14627
                     /locus_tag="O0305_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCY0273308.1"
                     /translation="MVKSIFLQDGEEIFVDDEDYERVNQYIWTKSYVDNVRRIHTKTL
                     NVSLSGFVLENGFQKIKNNDFTKNNITSIGYQQRWARPTRNTSSIYKGVYLNRKTKKW
                     SAVIKIDSKSKYLGSFVNEWEAAKAYNSAVDKYWDGQGYKNHKNQNDSIFEYEYKTYK
                     DQKRRRRGKSKFKGVYLTQSGYVAQITYKRKTYHIGWSKNIYETALMFNKINFYLHGS
                     DVILNDVPMTDELKEFISNWEIPDKIKALKGEDNGRSIVDKT"
     gene            14599..15402
                     /locus_tag="O0305_000122"
     CDS             14599..15402
                     /locus_tag="O0305_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCY0273309.1"
                     /translation="MGEVSWIKLKVGMFDDSKIKYIEALPERDTIITIWVKLLTLSGK
                     YNEQGYIMLSENLPYNEEMLANEFSRPINSIRLAIQTFETLGMIEKVNGVIKVTNWEK
                     HQNIEGLEKIRAQNRLRKQKQRENNRKLLNGHVTSRDSHATEEDKELDKELERDKEKD
                     IDKNLSSINSATDVTHEQFEEWWKLYDKKKDKKMSFTKFKSCLKKHSFEQIMQGTREY
                     LKTITDKQYQKYPKTFLTNESYMNDYSEEIKETGIDQLERMKYDESYWD"
     gene            15412..16191
                     /locus_tag="O0305_000123"
     CDS             15412..16191
                     /locus_tag="O0305_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0273310.1"
                     /translation="MKPLFSEKINESLKKYQPTHVEKGLKCKRCGSEYDLYKFAPTKK
                     HPNGYEYKDGCKCEIYEEYKRNKQRKINNIFNQSNVNPSLRDATVNNYKPQNEKQVKA
                     KQTAIEYVQGFSTKEPKSLILQGSYGTGKSHLAYAIAKAVKSKGHTVAFMHIPMLMDR
                     IKATYNKNAVETTDELVRLLSDIDLLVLDDMGVENTEHTLNKLFSIVDNRVGKNNIFT
                     TNFSDKELNQNMNWQRINSRMKHNARKVRVIGDDFRERDAW"
     gene            16185..16343
                     /locus_tag="O0305_000124"
     CDS             16185..16343
                     /locus_tag="O0305_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273311.1"
                     /translation="MVTKEFLKTKLECSDMYAQKLIDEAQGDENRLYDLFIQKLAERH
                     TRPAIVEY"
     gene            16356..16577
                     /locus_tag="O0305_000125"
     CDS             16356..16577
                     /locus_tag="O0305_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3269 family protein"
                     /protein_id="HCY0273312.1"
                     /translation="MPKEKYYLYREDGTEDIKVIKYKDNANEVYSLTGAHFSDEKKIM
                     TDSDLKRFKGAHGLLYEQELGLQATIFDI"
     gene            16588..16992
                     /locus_tag="O0305_000126"
     CDS             16588..16992
                     /locus_tag="O0305_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCY0273313.1"
                     /translation="MSKYNAKKVEYKGIVFDSKVECEYYQYLESNMNGTNYDRIEIQP
                     KFELQPKFGKQRSITYIADFSLWKEGKLVEVIDVKGKATEVANIKAKIFRYQYRDVNL
                     TWICKAPKYTGQEWMVYEDLVKVRRKRKREMK"
     gene            16997..17182
                     /locus_tag="O0305_000127"
     CDS             16997..17182
                     /locus_tag="O0305_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0273314.1"
                     /translation="MQQQAYINATIDIRIPTEVEYHHFDDVDDEKDMLAKRLDDNPDE
                     LLKYDNITIRHAYIEVE"
     gene            17183..17542
                     /locus_tag="O0305_000128"
     CDS             17183..17542
                     /locus_tag="O0305_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273315.1"
                     /translation="MSIVKINGKPYKFTEHENELIKKNGLTPGMVAKRVRGGWALLEA
                     LHTPYGMRLAEYKEIVLARIMQREAREREIARQRRKEAELRRKKPHLFNVPQKHSRDP
                     YWFDNTYNQMFKKWQEV"
     gene            17543..17791
                     /locus_tag="O0305_000129"
     CDS             17543..17791
                     /locus_tag="O0305_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273316.1"
                     /translation="MPKTDNARKEYLNQFFRSKRYLYQDNERVAHTHVVNGTYYFHGH
                     IVPDWQGVKKTFDTAEELGIYIKQHGLEYEEQKQLTLF"
     gene            17806..18207
                     /locus_tag="O0305_000130"
     CDS             17806..18207
                     /locus_tag="O0305_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273317.1"
                     /translation="MKIKVKKEMLLDELIKWARENPELSQGKIFFSTGFSDGFVRFHP
                     NTNKCSTSSFIPIDIPFIVDIEKEVTKDTVFDKLFEMYEMEGGVYETVLYANTSIKEC
                     LYGRRLPTKAFYILNDDMTMTLIWKDGELVV"
     gene            18204..18653
                     /locus_tag="O0305_000131"
     CDS             18204..18653
                     /locus_tag="O0305_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273318.1"
                     /translation="MMPKYRVWDEYTGRIHDVVGFDFIETEVHYENYAEAEALIHARD
                     FKDVELMQSTGLKDKNNNEIYAGDIVEFEDEILEMPDDESVIGTINRAVISIDVVNGI
                     QLKDFMFEGAVSENDYFEYIDIKSFLRYDCEVKGNIFESSHLLEVTE"
     gene            18650..19015
                     /locus_tag="O0305_000132"
     CDS             18650..19015
                     /locus_tag="O0305_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0273319.1"
                     /translation="MNYETGVQLGVMDARLKKMRKQRDEYKKQRDELIGDIAKLRERN
                     EELENMWRTVKNELLGRYEFYRFRLNELQIESRANKAVAINMGAKINASAILYRMDKL
                     DGTNEFYEFLGQMEEDTNE"
     gene            19008..19256
                     /locus_tag="O0305_000133"
     CDS             19008..19256
                     /locus_tag="O0305_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0273320.1"
                     /translation="MNNREQIEQSVISTSAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQEND"
     gene            19249..19779
                     /locus_tag="O0305_000134"
     CDS             19249..19779
                     /locus_tag="O0305_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0273321.1"
                     /translation="MTNTLTIDQLQELLQIQKDFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQQADNMEEISGYLDDGDFNDYI
                     ERVEIDFNDSDVVDEFMSTIDEMYESPYSSNLFLPFALANNYYTIDQLIDAYKKKMKR
                     NHERQDGTADAGKGYV"
     gene            19816..20022
                     /locus_tag="O0305_000135"
     CDS             19816..20022
                     /locus_tag="O0305_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0273322.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKANSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAIIKVSQLFENIRKCGK"
     gene            20019..20192
                     /locus_tag="O0305_000136"
     CDS             20019..20192
                     /locus_tag="O0305_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RinB"
                     /protein_id="HCY0273323.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDFAKL
                     SDQSDLMRAEVSE"
     gene            20193..20555
                     /locus_tag="O0305_000137"
     CDS             20193..20555
                     /locus_tag="O0305_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273324.1"
                     /translation="MDWIFFYTNIVIFIACVYTMYRRIEVSKKIGELRRDIKENEKAL
                     DNYKKENRPIEYIVELNDGVYFRKKHTDAFAQRTTYIITNNIFEAKSYDNLLSAKIDA
                     EILNGRVLKYKPNLEEVG"
     gene            20556..20702
                     /locus_tag="O0305_000138"
     CDS             20556..20702
                     /locus_tag="O0305_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273325.1"
                     /translation="MMWFIIAIILLVILLFGVMLQAEQLKGEVKVKEREIEILRSRLR
                     HFED"
     gene            20717..21136
                     /locus_tag="O0305_000139"
     CDS             20717..21136
                     /locus_tag="O0305_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0273326.1"
                     /translation="MSLRKSTQRYLESELSNYNYFDKDIARVRDEVLNPWSQQDTNIG
                     GDRVQSNVSVTEIKAIRVVNDRRLSQLARMKSAIEVVYNHSTVETQKLMELYYFKKPR
                     TLNLTGVAQEINVSKSTAYDMRKDILVRLADELGIIH"
     gene            21323..21763
                     /locus_tag="O0305_000140"
     CDS             21323..21763
                     /locus_tag="O0305_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0273327.1"
                     /translation="MNEKQKRFADEYIMNGCNGKKAAITAGYSKKTAESLASRLLRNV
                     NVSEYIKERLEQIQEERLMSITEALALSASIARGEPQEAYSKKYDHLNDEVEKEVTYT
                     ITPTFEERQRSIDHILKVHGAYIDKKEITQKNIEINIGEYDDEG"
     gene            21750..23027
                     /locus_tag="O0305_000141"
     CDS             21750..23027
                     /locus_tag="O0305_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCY0273328.1"
                     /translation="MTKVKLNFKKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHG
                     VIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNK
                     VELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQ
                     IFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPA
                     YYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDV
                     KKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILP
                     TKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHC
                     IDSLRYSVERFYRPVRKRTNVSSKVDTIKSLGL"
     gene            23038..24573
                     /locus_tag="O0305_000142"
     CDS             23038..24573
                     /locus_tag="O0305_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0273329.1"
                     /translation="MLKVNEFETDTDLRGNINYLFNDEANVVYTYDGTESDLLQNVNE
                     VSKYIEHHMDYQRPRLKVLSDYYEGKTKNLVELTRRKEEYMADNRVAHDYASYISDFI
                     NGYFLGNPIQYQDDDKDVLEAIEAFNDLNDVESHNRSLGLDLSIYGKAYELMIRNQDD
                     ETRLYKSDAMSTFIIYDNTVERNSIAGVRYLRTKPIDKTDEDEVFTVDLFTSHGVYRY
                     LTNRTNGLKLTPRENSFESHSFERMPITEFSNNERRKGDYEKVITLIDLYDNAESDTA
                     NYMSDLNDAMLLIKGNLNLDPVEVRKQKEANVLFLEPTVYADSEGRETEGSVDGGYIY
                     KQYDVQGTEAYKDRLNSDIHMFTNTPNMKDDNFSGTQSGEAMKYKLFGLEQRTKTKEG
                     LFTKGLRRRAKLLETILKNTRSIDANKDFNTVRYVYNRNLPKSLIEELKAYIDSGGKI
                     SQTTLMSLFSFFQDPELEVKKIEEDEKESIKKAQKGIYKDPRDINDDEQDDDTKDTVD
                     KKE"
     gene            24580..25575
                     /locus_tag="O0305_000143"
     CDS             24580..25575
                     /locus_tag="O0305_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCY0273330.1"
                     /translation="MPNKNTQEYWEERGRKAIENELKRDKTKAEEIERILNMMIKRIE
                     KEINAFIVKYGDFAGVTLQEAQKIIDEFDVKAFQEEAKRLVENKDFSDRANEELKKYN
                     TKMYVSREQMLKIQIEFLIAYATAQTELSMREYFESTAYRVFSDQAGILGEGVQVAKE
                     VIDTIVDTQFHGVVWSERLWTNTEAMKQEVEEIIANVVIRGRHPNEYVKDMRKHLNKF
                     EGTARQKTAAIKSLLYTESARVHAQSSIDSMKEISPEGYYMYIAKIDNRTTKVCKGLN
                     GEIFKVKDAKIGVNFYPMHINCRSDCALLPKSMWPKKPSKKRKTKYFGGKVKSGD"
     gene            25648..25818
                     /locus_tag="O0305_000144"
     CDS             25648..25818
                     /locus_tag="O0305_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273331.1"
                     /translation="MSNTDKYLRDIARELKGIRKELQKRNETVIIDANLDSVRSAVLA
                     DKEKSKYNEPLF"
     gene            25953..26567
                     /locus_tag="O0305_000145"
     CDS             25953..26567
                     /locus_tag="O0305_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCY0273332.1"
                     /translation="MDIQEKLKLKLQFFAEESDGDNGKSKDNNDDEGKDKQDKKTNSE
                     EEIEKRLQEEYNKRLKEELSRRMKQKEKEKQEAVDEAKRLAKMNKDQIAEYEREQMEK
                     ELEQLRSEKQLNEMRSEARKMLSEAEVDSSDEVVNLVVTDTAEQTKLNVEAFSNAVKK
                     AVNEAVKINARQSPLTGGDSFNHSTKNKPQNLAEIARQKRIIKN"
     gene            26581..27555
                     /locus_tag="O0305_000146"
     CDS             26581..27555
                     /locus_tag="O0305_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0273333.1"
                     /translation="MEQTQKLKLNLQHFASNNVKPKVFNPDNVMMHEKKDGTLMNEFT
                     TPILQEVMENSKIMQLGKYEPMEGTEKKFTFWADKPGAYWVGEGQKIETSKATWVNAT
                     MRAFKLGVILPVTKEFLNYTYSQFFEEMKPMIVEAFYKKFDEAGILNQGNNPFGKSIA
                     QSIEKTNKVIKGDFTQDNIIDLEALLEDDELEANAFISKTQNRSLLRKIVDPETKERI
                     YDRNSDTLDGLPVVNLKSSNLKRGELITGDFDKLIYGIPQLIEYKIDETAQLSTVKNE
                     DGTPVNLFEQDMVALRATMHVALHIADDKAFAKLVPADAKPSSNPGEV"
     gene            27577..27864
                     /locus_tag="O0305_000147"
     CDS             27577..27864
                     /locus_tag="O0305_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273334.1"
                     /translation="MPEIIGIVKVDFTDLEDNRHVYMKGHVYPRKGYDPTDERIKALA
                     SVENKRNEQMIYIVNDKLTKKELVEIASVAGLQVDEKQTKAEIINAFESLE"
     gene            27873..28205
                     /locus_tag="O0305_000148"
     CDS             27873..28205
                     /locus_tag="O0305_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCY0273335.1"
                     /translation="MTTLADVKKRIGLKDEKQDEQLEEIIKSCESQLLSMLPIEVEQI
                     PERFSYMIKEVAVKRYNRIGAEGMTSEAVDGRSNAYELNDFKEYEAIIDNYFNARTRT
                     KKGKAVFF"
     gene            28202..28504
                     /locus_tag="O0305_000149"
     CDS             28202..28504
                     /locus_tag="O0305_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273336.1"
                     /translation="MRYEDRAVFQSEQAATYNPKTSKKENTLITYDAIPCNINPISRA
                     RKQLEFGDVKNDVSVLRIKESISYPVSHVLVNGIRYKIVDTRIYRHETSYYIEEVN"
     gene            28504..28851
                     /locus_tag="O0305_000150"
     CDS             28504..28851
                     /locus_tag="O0305_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0273337.1"
                     /translation="MNIDGLDALLNQFHDMKTNIDDDVDDTLQEKAKEYVVRAKLKAR
                     EVMNKGYWTGNLSRNIRYKKTGDLQYTITSHAAYSGFLEFGTRYMEAEPFMWPVYEVI
                     RKSTVEELKALFE"
     gene            28863..29246
                     /locus_tag="O0305_000151"
     CDS             28863..29246
                     /locus_tag="O0305_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273338.1"
                     /translation="MTPNLQLYNKAYETLQGYGFPVISRKEMQQEIPYPFFVIKMPES
                     NRSKYTFDSYSGDTNLVIDIWSVSDDLGHHDGLVKRCIDDLTPSVKTNDYDFEEDDTN
                     ITQLVDDTTNQELLHTSVTISYKTF"
     gene            29265..29846
                     /locus_tag="O0305_000152"
     CDS             29265..29846
                     /locus_tag="O0305_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HCY0273339.1"
                     /translation="MANMKNSNDRIILFRKAGEKVDATKMLFLTEYGLSHEADTDTED
                     TMDGSYNTGGSVESTMSGTAKMFYGDDFADEIEDAVVDRVLYEAWEVESRIPGKNGDA
                     TKFKAKYFQGFHNKFELKAEANGIDEYEYEYGVNGRFQRGFATLPEAVTKKLKATGYR
                     FHDTTKADALTGEDLTAIPQPKVDSSTVTPGEV"
     gene            29908..30273
                     /locus_tag="O0305_000153"
     CDS             29908..30273
                     /locus_tag="O0305_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCY0273340.1"
                     /translation="MNVEINGKSLELSFGFKFLREIDNRLGLKVEQASIGQGVSMLPV
                     GLESGNPVVIGEVLIAATSHLKKQAITINNIDEALDEIAENIGLEEFGSDILTELGKR
                     PMTRNLVEVVETEEKPAGA"
     gene            30303..30647
                     /locus_tag="O0305_000154"
     CDS             30303..30647
                     /locus_tag="O0305_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273341.1"
                     /translation="MSTLGITDLNVIEQMTLTEYNYRMYAKEYEMLTQEFERYKLAFA
                     IRDAAATKNVGTENKPKEEYVFNNANDVLPYEENIQRLNEGKDIRFSSERDEYEPQNN
                     EFFKVIAEFNKQ"
     gene            30664..34263
                     /locus_tag="O0305_000155"
     CDS             30664..34263
                     /locus_tag="O0305_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273342.1"
                     /translation="MTEYKIKATIEASVAKFKRQIDSAVKAVQKYKRTADKTKDVELS
                     ANDKKLQKTIKVAKKTLDSFGNEKAKAKLDAKIEDLKQKVLEASFELNQLDSKEVTPE
                     VKLEKQKLIKDIIETEAKLSELEKKRVNIDVNADNSKFNRVLKVSKASLEALNRSKAK
                     AVIDVDNSVANSKIKRTKEELKSIPNKTRSRLNVDTGLSIPTIYAFKKSLDALPNKKT
                     TKVDVDTNGLKKAYTYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILI
                     PVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQ
                     ASSATNEYKKALDGVKSAWTDIIKQNQSAIFTSLANGLNTVKTAMQSLQPFFSGISRG
                     MEEASQSVLKWAQNSGVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLF
                     QWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPVIGNIFKNVFVGINNLMNA
                     FSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYVQTNGPLIMQLIGNIARGLV
                     AFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAV
                     SNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIR
                     NAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVN
                     VWNVLKAVASVVWNAILKAITTAVTNVYTFIMIVWNQIVAYLQGLWNGIIAIATTVWN
                     LLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIM
                     TIAMTIWNAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIMTIAMTIWNAIWTFL
                     QTLWNTIVTVATKVWNAITTAISAALQAAWSFISNIWNTIWSFLSGILTTIWNKVVSI
                     FTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVIQGFVNVVNKVSQGMTN
                     AVNKIKSFIGDFVSAGADMIRGLIRGIGQMAGQLVDAAKNVAKKALDAAKSALGIHSP
                     SREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPTLNAPDISSITGNLS
                     NLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRNAKRNSEYYL"
     gene            34276..35223
                     /locus_tag="O0305_000156"
     CDS             34276..35223
                     /locus_tag="O0305_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0273343.1"
                     /translation="MDIELTKKDGTVIKLSEYGFIVNDIVIDSMQINTKYQDKESMNG
                     RILMGSNYISRDIVVPCFCKVKNRSDIAYMRDMLYSLTTDIEPMYLREIRRKEELNYR
                     FTQPTSDDYVKLDKNNFPDYEYSRHDQQIYVNGKQYKVIFNGVINPKQKGNKVSFELK
                     FETTELPYGESIGTSLELEENKKVGLWSFDFNIDWHAGGDKRQYTFENVSKDTVYYHG
                     SAPNDQFNMYKKITIILGEDTELFVWNLTHAEIMKIEGIKLKAGDRIVYDSFRVYKNG
                     VEISTETNIAQPKFKYGANKFEFNQTVQKVQFDLKFYYK"
     gene            35232..37133
                     /locus_tag="O0305_000157"
     CDS             35232..37133
                     /locus_tag="O0305_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0273344.1"
                     /translation="MTITIKPPKGNGAPVPVETTLVKKVNADGVLTFDILEDKYTYEV
                     INAIGKRWIVSHVEGENDKKEYVITVIDRKSEGDRQLVECTAREIPIDKLMIDRIYVN
                     VTGSFTVERYFNIVFQGTGMLFEVEGKVKSSKFENGGEGDTRLEMFKKGLEHFGLEYK
                     ITYDKKKSRYKFVLTPFANQKASYFISDEVNANAIKLEEDASNFATFIRGYGSYSGEE
                     TYEHAGLVMEARSALAEIYGDIHAEPFKDGKVTDQETMDKELQSRLKKSLKQSLSLDF
                     LVLREAYPEADPQPGDIVQIKSTILGLNDLVRIVEIKTIRDINNVIVKQDVTLGEFNR
                     EQRYMKKVNTAANYVSGLNDVNLSNPSKAAENLKSKVASIAKSTLDLMSRTDLIEDKQ
                     QKVSSKTVTTSDGTIVHDFVDKSNIKDVKTIGTIGDSVARGSHAKVNFTEMLGNKLNA
                     KITNLAKGGATMATVPIGKDAVENSIYRQAEQIRGDLIILQGTDDDWLHGYWAGVPIG
                     TDKTDTKTFYGAFCSAIEVIRKNNPASKILVMTATRQCPMSGTTIRRKDTDKNKLGLT
                     LEDYVNAQILACSELDVPVYDAYHTDYFKPYNPAFRKSSMPDGLHPNERGHEVIMYEL
                     IKNYYQFYG"
     gene            37148..39058
                     /locus_tag="O0305_000158"
     CDS             37148..39058
                     /locus_tag="O0305_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273345.1"
                     /translation="MSNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRN
                     KEVFAHDGEQIKYEHLNVTSNVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGY
                     GHKTLQDRLYHDYSTLDVFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYT
                     NAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYI
                     DGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPVENL
                     MIFRREYKPTERQLKNSLNFVEVRSADDIDKGIDKVLYQMDIPMEYTSDTQPMQGITY
                     DAGILYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNNNFKGDFQEAEGLDMY
                     YDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLP
                     IQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFLDVLPGHYNGALRQVLT
                     RNSTGRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLDFYI
                     TTEESNRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGV
                     GKWSLFEGKVVE"
     gene            39058..40881
                     /locus_tag="O0305_000159"
     CDS             39058..40881
                     /locus_tag="O0305_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCY0273346.1"
                     /translation="MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFA
                     VTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLK
                     HSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGK
                     DFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFDK
                     IVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESYEQSIDSVLS
                     AVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVD
                     NNTARATWYPDDSNDEYTKYKISGTWYPFYKKNDGDLTKQFVEETSNNALNQAKQYVD
                     NKFGTTSWQQHKLTEPNGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGY
                     LSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGT
                     TINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECL
                     LSKTSSTTLRIDNDVYFDLGKTSGSGTNANKVTITKIMGWK"
     gene            40881..41258
                     /locus_tag="O0305_000160"
     CDS             40881..41258
                     /locus_tag="O0305_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0273347.1"
                     /translation="MKITVNDKNEVIGYVNTGGLRNSLDVDDNNVPIKFKEEFEPRKF
                     VFTNGEIKYNSNFEKEDVPNASSQQSESDLSDEELRGMVASMQMQVAQVNVLTMELAQ
                     QNAMLTQQLTELKTNKTSTEGDV"
     gene            41261..41434
                     /locus_tag="O0305_000161"
     CDS             41261..41434
                     /locus_tag="O0305_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0273348.1"
                     /translation="MKMIYPTFKDIKTFYVWGYYKNEQIKWYVDKGLIDKEEYALITG
                     EKYPETKDEKSQV"
     gene            41475..41774
                     /locus_tag="O0305_000162"
     CDS             41475..41774
                     /locus_tag="O0305_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0273349.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRAQEKIYDK
                     LDRNFEELKRDKVEDEKNKEKNAKNIRDIKMWILGLIGTIFSTIVVALLRTIFGI"
     gene            41911..43809
                     /locus_tag="O0305_000163"
     CDS             41911..43809
                     /locus_tag="O0305_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0273350.1"
                     /translation="MGLPNPKTRKPTASEVVEWALYIAKKKIAIDVPGSGMGAQCWDL
                     PNYLLDKYWGFRTWGNADAMAQKSNYRGRDFKIIRNTKDFVPQPGDWGVWTGGWAGHV
                     NIVVGPCTKDYWYGVDQNWYTNNATGSPPYKIKHSYHDGPGGGVKYFVRPPYHPEKST
                     PAPKPEDDSDDNEKNNKKVPIWKDVTTIKYTISSQEVNYPEYIYHFIVEGNRRLEKPK
                     GIMIRNAQTMSSVESLYNSRKKYKQDVEYPHFYVDRHNIWAPRRAVFEVPNEADYIVI
                     DVCEDYSASKNEFIFNEIHAMVVAVDMMIKYEIPLSIENLKVDDSIWRSMLEHVNWNM
                     IDNGVPPKDKYEALEKALLNIFKNREKLLNSITKPTVTKSRIKVMVDNKNADIANVRD
                     SSPTANNGSASKQPQIITETSPYTFKQALDKQMARGNPKKSNAWGWANATRAQTSSAM
                     NVKRIWESNTQCYQMLNLGKYQGVSVSALNKILKGKGTLNNQGKAFAEACKKHNINEI
                     YLIAHAFLESGYGTSNFANGKDGVYNYFGIGAYDNNPNYAMTFARNKGWTSPAKAIMG
                     GASFVRKDYINKGQNTLYRIRWNPKNPATHQYATAIEWCQHQASTIAKLYKQIGLKGI
                     YFTRDKYK"
     gene            43822..45060
                     /locus_tag="O0305_000164"
     CDS             43822..45060
                     /locus_tag="O0305_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCY0273351.1"
                     /translation="MYKIKDVETRIKNDGVDLGDIGCRFYTEDENTASIRIGINDKQG
                     RIDLKAHGLTPRLHLFMEDGSIFKNEPLIIDDVVKGFLTYKIPKKVIKHAGYVRCKLF
                     LEKEEEKIHVANFSFNIVDSGIESAVAKEIDVKLVDDAITRILKDNATDLLSKDFKEK
                     IDKDVISYIEKNESRFKGAKGDKGEPGQPGAKGDTGKKGEQGTPGKNGTVVSINPDTK
                     MWQIDGKDTDIKAEPELLDKINIANVEGLENKLQEVEKIKDTTLNDSKTYTDSKIAEL
                     VDSAPESMNTLRELAEAIQNNSISESVLQQIGSKVSTEDFERFKQSLNSLYADKNHSH
                     TIKQIEGLENALSKKSDINHSHDERYLLSSNAFTKEEADKLYQPIGSSQPSLNIWTGS
                     ETEYNYLYQKDPNTLYLIKG"
     gene            45065..45460
                     /locus_tag="O0305_000165"
     CDS             45065..45460
                     /locus_tag="O0305_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273352.1"
                     /translation="MEGNFKNVKKLIYEGEEYTKVYAGNIQVWKKPSSFVIKPLPKNK
                     YPDSIEESTAKWTINGVEPNKSYQVTIENVRSGIMRVSQTNLGSSDLGISGVNSGVAS
                     KNINFSNPSGMLYVTISDVYSGSPTLTIE"
BASE COUNT        16952 a         6662 c         8987 g        12897 t
ORIGIN      
        1 tttaagttgt ttatattaag gtataagtaa gttataatta actgaacgca ttattacaaa
       61 gtctttttga ctacaaatta aaattattat aaactagtta agaaaacttt atattttacg
      121 gagggaatat aaaatggcat caacattaga aatcaaagac ctacatgtgt ctattgagga
      181 taaagaaatc ttaaaaggtg ttaacttgac aattaacact gatgaaatac atgcgattat
      241 gggaccaaac gggacaggta aatcaacttt atcatctgca attatgggac acccaagcta
      301 tgaagtaact aaaggagaag tacttttaga cggtgtaaat attttagaat tagaagttga
      361 tgaaagagca aaagcaggat tattcttggc aatgcaatat ccatcagaaa ttacaggtgt
      421 tacaaatgct gatttcatgc gttcagcaat caatgcgaaa cgtgaagaag gacaagaaat
      481 caacttaatg caatttatta agaaattaga taaaaacatg gattttctag acatagataa
      541 agacatggca caacgttatt taaatgaagg tttctcaggt ggagagaaga aacgtaacga
      601 aatcttacaa ttaatgatgt tagaacctaa gtttgcaatc ttagatgaaa tcgattcagg
      661 gttagacatc gatgcattaa aagttgtatc taaaggtatt aaccaaatgc gtggggaaaa
      721 ctttggtgca ttaatgatta cacactatca acgattatta aattacatta ctcctgataa
      781 agtacatgta atgtatgctg gtaaagtcgt taaatctggt ggtccagaat tagcaaaacg
      841 tcttgaagaa gaaggatatg aatgggttaa agaagagttc ggttcagctg aataatctta
      901 ttaatacagt atccatgaga tgttcatcta tatatgatga aaatgaacat ttatacgaaa
      961 tagtaaattt catcaagtag gaggaaaaag ttatgacaac tgatattttg aacatttctg
     1021 aagaacaact tgttgattat tctaaagccc acaatgaacc ttcttggatg acagaattac
     1081 gtaaaaaagc tttgaaatta acagaaactt tagaaatgcc aaaacctgat aaaacaaaat
     1141 taagaaaatg ggattttgat tcttttaaac aacacgatgt aaaaggtgat gtttatcaat
     1201 ctttatcaca attacctgag tcagtaagag aaattattga cgtagatcat tctaaaaact
     1261 tagtaattca acataataat acgattgcgt acacacaagt tgatgataat gcatcgaaag
     1321 atggcgttat cgttgaaggt ttagcagacg ctcttatgaa ccatagtgat ttagtacaaa
     1381 agtactttat gaaagatgca gtaacagtag atgaacatcg tatcacagcg ctacacacgg
     1441 cattagttaa tggtggcgta tttgtttatg ttcctaaaaa tgtagttgta gaacatccag
     1501 tacaatacgt tgtgttgcac gacgacgaaa atgcaagctt ttataaccat gttatcatcg
     1561 ttactgaaga aagcgccgaa gtcacatatg ttgaaaatta cttatcaaat gcatctggtg
     1621 aaggaaatca attaaatatt atttctgaag tgattgctgg tgcaaattca aatatcacat
     1681 atggctcagt ggactatatg gataaaggct ttacaggtca tatcattcga cgtggtatta
     1741 ctgaagcgga tgcctcaatt aattgggcac taggtttaat gaatgagggt agccaaatta
     1801 ttgataatac aacaaattta tttggtgatc gttcaacaag ttcacttaaa tcagtagttg
     1861 taggtacagg cgaacaaaaa attaatctaa catctaaaat cgtacaatat ggtaaagaaa
     1921 cagatggtta tatccttaaa catggtgtta tgaaagaaca tgcatcgtct gtatttaatg
     1981 gtatcggcta cattaagcat ggtggaacta aatcaattgc taatcaggaa tcacgtgtat
     2041 taatgttatc tgaacatgct cgtggtgacg cgaatcctat tttattaatt gatgaagatg
     2101 atgtacaagc tggtcatgct gcatcagtag gtcgtgttga tccagatcaa ctttactatt
     2161 taatgagtcg tggtatttct caaagagaag cggaacgtct tgttatacat ggtttcttag
     2221 atccagtagt acgtgaatta cctatcgaag acgttaaacg tcaattgaga gaagtaattg
     2281 aacgcaaagt ttctaaataa tattttgaaa ataaaagttt gtaatagata tagactgtcg
     2341 atattggtat aagactaata caacgtcagt atttaaatga ttaggatttt tatttaagaa
     2401 aggtcgtgaa tgaagtggcc gaacactcat ttgacgttaa tgaagtaatc aaggattttc
     2461 cgatattaga tcaaaaagtc aatggcaaac gtttagcata tcttgattca acagcgacaa
     2521 gtcaaacgcc tgtgcaagtg ttaaatgttt tagaagatta ctacaagcgt tataattcaa
     2581 acgttcatcg tggtgttcat acattaggat cattggcaac tgatggttat gaaaatgccc
     2641 gtgaaaccgt tcgtcgtttt attaatgcga agtattttga agaaatcatt tttacacgcg
     2701 gaacaactgc gtcgattaac cttgtagcac atagctatgg tgatgcaaat gttgaagagg
     2761 gcgatgaaat tgttgtcact gaaatggaac atcatgccaa tattgttcct tggcaacagt
     2821 tagcaaagcg taaaaatgcg acattgaaat ttataccaat gacagctgac ggtgaattaa
     2881 acatcgagga tattaagcaa acgattaatg ataaaacaaa gatcgttgct attgcacata
     2941 tatctaatgt gctcggtaca attaatgatg ttaaaaccat tgcagaaata gctcatcaac
     3001 atggtgcaat tatcagtgtt gatggggcgc aagcagcacc acatatgaaa cttgatatgc
     3061 aagaaatgaa tgctgatttt tatagtttta gtggtcataa aatgcttgga ccaacaggta
     3121 ttggcgtatt atttggtaaa cgtgagttac tacaaaaaat ggaaccgatt gagttcggtg
     3181 gcgacatgat tgattttgta agtaagtatg atgcaacatg ggctgattta cctactaaat
     3241 ttgaggcggg tactccatta attgctcaag caattgggct tgcagaagct attcgctatt
     3301 tagaacgcat aggttttgat gcaattcata aatatgaaca agaattaacg atatatgctt
     3361 atgagcaaat gtctgcaatt gaaggaattg aaatttatgg cccgccaaag gatcgtcgtg
     3421 caggtgtaat aacgtttaat ttacaagatg tacatccaca cgatgttgct acagccgtag
     3481 atacagaagg tgtagcggtt agagctgggc atcattgtgc gcaaccgtta atgaaatggt
     3541 taaatgtgtc ttcaacagct agagcgagtt tttatatata caacacgaaa gaagacgttg
     3601 atcagttaat aaatgccttg aaacaaacga aggagttttt ctcttatgaa ttttaataat
     3661 ctagatcaat tatatagatc tgtcattatg gatcattata aaaatcctag aaataaaggt
     3721 gtattagata acgggtctat gacagtagat atgaataacc cgacatgcgg tgaccgtata
     3781 cgactaacat ttgatataga agacggcatt ataaaagatg ctaagtttga aggtgaaggt
     3841 tgttcgattt caatggcaag tgcatcgatg atgacacaag ctgttaaagg tcattcactt
     3901 ggagaagcaa tgcaaatgag ccaagaattt acgaaaatga tgcttggtga agactatgtg
     3961 attacagaag aaatgggaga tattgaagca ttgcaaggtg tatctcaatt cccagctcgt
     4021 attaaatgtg ccacattagc ttggaaagca ttggaaaaag gtactgttgc taaagaaggt
     4081 aaagcagaag gtacgactga agaagaatag aatgctgtta atcatagata attttgatat
     4141 tagacatata aaagtataaa aatttttata agatgtcatg tcattgttat aatatggttt
     4201 acatcatgaa ttaaaaactt acgcacgccg ttgtaaatat atttttaagg agtgattgaa
     4261 atggctaaaa aagcacctga tgttggggat tataaatatg gattccacga cgatgatgtg
     4321 tccattttca gatcagaacg tggtttaact gagaatatcg ttagagaaat ttctaacatg
     4381 aaaaatgagc cggaatggat gttagatttc cgtcttaaat cattaaaatt gttttataaa
     4441 atgccaatgc ctcaatgggg tggcgactta tcagaattga atttcgatga cattacttac
     4501 tatgtaaagc cttcagaaca agctgaacgt tcatgggatg aagtgccaga agaaattaaa
     4561 agaactttcg ataaattagg aattcctgaa gctgaacaaa aatatttagc tggtgtttct
     4621 gctcaatatg aatctgaagt tgtttaccat aatatggaaa aagaacttga agaaaaaggt
     4681 attatcttta aagatacaga tagtgcttta caagaaaatg aagaattatt caaaaaatac
     4741 tttgcttctg tagtacctgc agcagataac aaatttgcgg cgttaaactc agcagtatgg
     4801 tcaggcggtt cgttcattta tgtacctaaa aatatcaaac tagatacgcc actacaagct
     4861 tatttccgta ttaactctga gaacatgggt caatttgaac gtacattaat cattgctgat
     4921 gaaggtgctt ctgtacatta cgtagaaggt tgtactgcac cagtttatac aactagttct
     4981 ttacactctg ctgttgtgga aatcattgtg cataaagatg cgcacgttcg ttatactacg
     5041 attcaaaact gggcgaacaa tgtatacaat ttagttacaa aacgtacttt tgtttatgaa
     5101 aacggaaata tggaatgggt agatggtaac ttaggttcta agttaacgat gaaatatcca
     5161 aactgtgttc ttttaggtga aggtgcaaaa ggtagtacat tatctattgc atttgctggt
     5221 aaaggacaag ttcaagatgc cggtgctaaa atgattcata aagcacctaa tacatcttct
     5281 acaattgttt ctaaatctat ttctaaaaat ggtggtaaag ttatttatcg cggtattgtt
     5341 cattttggac gtaaagcaaa aggtgctcgt tcaaatattg aatgtgatac attaatctta
     5401 gataacgaat caacatcaga tacaattcca tataacgaag tattcaacga tcaaatatca
     5461 ttagaacatg aagccaaggt ttcaaaagtt tctgaagaac aattattcta tctaatgagt
     5521 cgtggtattt ctgaagaaga agcgacagaa atgattgtta tgggattcat cgaaccattt
     5581 acaaaagaac ttccaatgga atacgcggtc gaaatgaacc gtttaatcaa gttcgaaatg
     5641 gaaggtagta ttggatagct ttaaaccgcg ttgttaagcc attcttgact tccggaaatg
     5701 gctattgata ccattttgat accatttact tgtcaaaaat ggctattgca tcgtgttttt
     5761 tctgagtata taaatggctg taagtgccca tcgtttcagt gatttgagca tgtctcatga
     5821 gtgactgtaa aacgaaaata tctacaccat tatttgcaag ataagatgca taagaatgtc
     5881 ttaacgcgtg aatgttataa tgggggaaag ctttttggaa tttcttttga acatgactat
     5941 aatgtttggg agccattcct ccgaaaataa aataactacg ttcatcaaaa tatttgttta
     6001 actctttttc acgttggtgt cgttcagtta acattgtatt gatgaattta ggtaaaggaa
     6061 caatatcctc tgaactatct gtttttggtc ttggatatat agttctatta gagatgtcca
     6121 ttgttttatt tatggatatc tcttttttgt atttattgta gtctgtccaa acaagcgcca
     6181 tagcttcgcc aatcctcaaa cctgtataaa acattaatgt aaataactct ctgtaatctt
     6241 gctcttcaat atctttgatt ctttcttcaa attcttcacg catcataaac ttaggttttg
     6301 gctttacacg cggaataggt ttaattgata ttgttggatc tgtacgtaat ccaaagtatt
     6361 ttttagcata attaattaca actttaaaac ctgaccaaat tgtacgagca gaatttgttg
     6421 acgctacatt ctctattaga tatttacgaa actcttggca ttgattttgt gttatcttat
     6481 tcatttttat gtgcccaaac ttagctttaa agtgtttatg atattcattt tgcttgcgtc
     6541 gttttgtttt aggtctcaaa tcgctatttt ctaaatagtg atgaaaaaca taatcaaaag
     6601 tttttgaatc gctatatcct tcgtttacgt cattcaaaaa aatagcctct gctctcttag
     6661 cttcacgctt agttgaaaaa ccgcgttgca tcttacgttt gttattaccg tatacatctt
     6721 tatatctaat agaaaaatac catttacctg tattatcatc cttatatact ggcattttgc
     6781 ttctccctcc tcaaaattgg caaaaaaata ataagggtag gcgggctacc cgaaatttta
     6841 ttgttgaatc acttcgctat tttgacgttt gaaattgtcg aaatcatttt gtgctttctt
     6901 ccatgaatta tagtcttgtc cgtcttgtac tgcccatgaa ccacctatac cggcagtatg
     6961 gccaccattc tgacgtttgt tttcttctgt tgctctttta gcttcttgat aagcgttata
     7021 agatgtgtca cttgaaaact catctttcac tggtgcattg ttgtttttat tagaagtggg
     7081 attattttgt gtttgatttt gtttaggtgc gttattagtt tgttgatgat cattaacatt
     7141 tgtgttgtta tcgttgttta cttgattatt gttatcgttt tgattagcat tttctttttt
     7201 cacttctgct ttgtctttag tttctttctt tttgtctttg ttctctttct ttgtttccgc
     7261 tttcttgctt tcctctttct tatcgccgtc gttgctacca catgcaccta atactaacgc
     7321 gctagctaaa attaaatata ataatctttt catgttttac actcctttat ttgctatttg
     7381 ttttaataaa tctatgactt cgttgttttg ctcgataatt ctattatttt gctttattag
     7441 ttcgtctcgt tgagctatag agacaaagtt ttgttttaat tgcgtatcgt agaatacgaa
     7501 tttagcttgt ttatctacgt ttgttgtgaa tgtacctaaa ccgttgtaga ctttcaatag
     7561 tgtagggtta atattttgct tttgatatgc gtaagtcgta acgtcggtag cttctttaat
     7621 accttgtccg tttaaacttt tagctgattt tgattcgtat tcttcgttag tgtttttaaa
     7681 attttcagat ttataaagtt ggatatcaag ttcttttcct tctttaaaat catttaatat
     7741 ctttcttttt tcatctgttg tcattttttt atacatgtca atctttctat tgcttaactt
     7801 actaaacatt tttgtttctg ttagaatttc tttaaaagtt aatttatctc ctgccatttt
     7861 caatttctcc ttcatttggt ttatattaaa gcgccacata gacgctatta atcaaaaatt
     7921 cgatagttat aaataacttt gcctatcact tcgatttcat caatagaatc taaatcgtaa
     7981 gaattagttt taaattcatc tgaatagctt actgggtcta aatgtagttt tgtttcagta
     8041 cgtctcacac gtttaactgt atattcacca cctagacgca atacaaggat gtcgttgctg
     8101 ttaagtttat gatcacaaga ctttctataa tcatggacaa ttatataaga accgttagcg
     8161 agtattttat tcatgctatc tccgtttatt tttagtgcta tacattcgct aggtttacga
     8221 ccgttaaaag caaatggtgg aacttttaat ttttcatttt caattgcaac ttcttcgaaa
     8281 tttccagcag aaactttacc gaaatatgga acctcgattt cgctatcaaa ttcgggtaaa
     8341 acaatttctt caatttctcc taagagataa cctttagaaa cattgaacaa acttgaaatt
     8401 ttttcgacca tacccattct aggttcagtt cttccgcttt cccacattct tatagtacct
     8461 tcggaaacat ctaattttct agccatctca actttagaca atctattgtt caatctgatt
     8521 tcttttatgg aatttttgaa agccattttg ttttccttcc ttatatataa tgttttttac
     8581 acttttatta tactatgaaa aatcgtaatt gcaaccctta aaatacgatt tacgaaaata
     8641 aaaatacgta aaattctaaa ataattacga aaaacacttg aaatcgtatt taaattacga
     8701 tatactttag tcagaactta acaaggaggt taaaaaatga actacatcaa acaaagtctg
     8761 aaattagatg aatggcgaaa acgaaaaggt tatacccagt cgtcttttgc tgaaaaactt
     8821 ggcatttcac catctactta taacatttgg gaaaataacc cagaaatgat taaacctaga
     8881 gatgctttta gaattgccaa aacattaaat atctctattg atgagattat ttttttaaaa
     8941 gatgaatcgt attttaaata cgttttagtc gaagggaaac aaatgtctta aaaggaggaa
     9001 tgaacgatgc aagcattaca aacatttaat tttaaagagc taccagtaag aacagtggaa
     9061 attgaaaacg aaccttattt tgtaggaaaa gatattgctg agattttagg gtatgcaagg
     9121 acagacaatg ccatcagaaa tcatgttgat agcgaggaca agctgacgca ccaatttagt
     9181 gcatcaggtc aaaacagaaa tatgatcatt atcaacgaat caggattata cagtttaatc
     9241 ttcgatgctt ctaaacaaag taaaaacgaa aacattagag aaaccgctcg gaaattcaaa
     9301 cgatgggtaa catcagatgt cctaccagct attcgaaaac acggtatata cgcaacagac
     9361 aatgtaattg aacaaacatt aaaagatcca gactacatca ttacagtgtt gactgagtat
     9421 aagaaagaaa aagagcaaaa cttactttta caacaagaaa tcggagaact aaaacccaaa
     9481 gcagactatg tagatgaaat cttaaagtca actggcacat tagccacaac tcaaatcgcg
     9541 gcagactacg gtatatcagc acaaaagtta aacaaactac tacacgaagc tagactacaa
     9601 cgaaaagtaa ataaacagtg ggtgctttac tcagaacaca tgggcaagag ttacacagat
     9661 tcagacacta taacaattgt gcgttctgat ggcagagaag acacagtttt acaaactaga
     9721 tggacacaaa aaggcagatt gaaaatacat gaaatcatga ctgaattcgg ttatgaagct
     9781 aatttagggg gagcgtaaat gacaccagaa caaaaagaaa agctaaacaa tatagtatta
     9841 acactttatg cagttaaaga aaacaaaagt caaacataca cacacaaaga tactcttact
     9901 gtgacatatg caggcgagat tgagcacact tacgaagtcg acagagagaa acaccttgaa
     9961 tcaatgattg agtgggcaat tgaccaaatc gaacagcact ttgatttaga cgaagaagaa
    10021 taacacacaa ttgaacaaac aacttaatag gaggaattac aaatgaacgc actatacaaa
    10081 acaaccctcc tcaccacaat ggcagttgtg acgtggaagg tttggaagat tgaacgaaat
    10141 acgagaaagc ctgtaatcaa tcggaatgat tttagtaaag agtctacagc agaaacgatt
    10201 gagcgacaca gtgatcctga ttcaggaata aaactactta aggcattttc cgacttcact
    10261 aaacaagctg aaaagcaaaa acctacacta ggagaagttt atagacggaa caaacctgaa
    10321 ttaccaaccg ttactttaga cgaaaacgga ctgtttataa atgattttag ggtgccttat
    10381 gtacttgagg aaggggttaa cgtaaagaaa tctatgaaca acctatataa ggtcagtttg
    10441 gactttttcg ctaaaagtat tattgcagat aattacgaag cagataaccc agagaatcaa
    10501 cagttatttt aaaggaggaa aagatatgat gaaaaatagt ttgcaagcta aagaacttgc
    10561 ggtaatttta tctgtttcaa aatccaaagc aggacaaata ataagagaac tgaataaaga
    10621 gcttgaagac gaagggtaca ttgcgatacg aggcagaata ccagtccaat tagctaggaa
    10681 aaaattccct tatcacgact tatcagacga gagaataatg gaggagttga aaaaagaaaa
    10741 tgagtaacat ttataaaagc tatctattag cagtattatg cttcacagtc ttagcaattg
    10801 tactcatgcc gtttctatac ttcactacag cgtggtcaat tgcaggattc gcaagtatag
    10861 ggacattcat attttataaa gaatactttt atggggtgga tgattaaatg acttggtttg
    10921 aagaatacgt taaacctagt gtggaatggg aaagaaaggc agaacaagct gttttaagtg
    10981 atgatgaagt taaaacgatc actgaatata gaaagaagta caacaacccg catatttaca
    11041 tgtcggctca gaacagaaat tatcttgttg aatatttaga tagacatact ggagacatag
    11101 tattacacaa tttaaaactt aagaaatcat ccagaagaag agtgcatcaa tatttaatgg
    11161 tcggccaaat agtagtgccg ggcgaaccaa aaggcacaat ttatgaagca tctctgataa
    11221 taagataaaa aaactgctac ttgcgccaac aagtaacaga gacaaacgat tagcaaaatt
    11281 aattcacgtt caatataaaa cgaaaaacgg aggaagtcaa gatgtattac gaaataggcg
    11341 atgtatgtca gaaggtaatt aatgtagacg gatttgattt taaattagca gttaagaaga
    11401 aggaccacag cattctggtg aatatcttag atttagaaga taagtttatc gacggcataa
    11461 acataactaa tgagaacgat ctatacacag cattagacat attaaatcaa tctatttacg
    11521 aatggattga agaaaacgca gatgattatg acagactaat taacttagtc atgaaatggt
    11581 aggaggttgc tatgaagcag actgtaactt atatcattcg tcatagggat atgccaattt
    11641 atataactaa caaaccaact gataacaatt cagatattag ttactccaca aatagaaata
    11701 gagctaggga gtttaacggt atggaagaag cgagtatcaa tatggattat cacaaagcaa
    11761 tcaagaaaac agtgacagaa acaattgagt acgaggaggt agaacatgac tgaacaaact
    11821 aatcaagatg tcgatatttt aacgcaacta gatgtaaaag acatcagcaa acaaaatgca
    11881 aacaagtttt ataaatttgc gatatacggc aagttcggga ctggtaaaac tacgttttta
    11941 acaaaagata acaatgcctt agtactagat ataaatgagg acggaacaac ggtaacagaa
    12001 gatggggcag ttgtgcagat taagaattat aagcatttta gtgcagtggt taaaatgttg
    12061 cctaaaatta ttgaacaact aagagaaaac ggaaaacaaa ttgatgttgt agtgattgaa
    12121 acaatccaaa agctacgtga tatcactatg gacgacatca tggacggaaa attaaagaaa
    12181 ccaacattta atgaatgggg cgagtgtgct tcacgcattg taagtattta tcgttatatt
    12241 tctaaattac aagaacatta tcaattccat cttgctataa gtggacacga gggaattaac
    12301 aaagacaaag atgatgaggg tagcactatc aatccaacaa tcacgataga ggcacaagat
    12361 caaataaaaa aagcggtcat cagtcaatct gatgtgttag caagaatgac aatagaagaa
    12421 catgagcaag acggcgaaaa agcttatcaa tatgttctta acgctgaacc atcaaactta
    12481 ttcgagacaa agataagaca ctcaagcaac attaaaatta acaacaaacg tttcattaat
    12541 ccaagtatta acgacgtagt acaagcaatc agaaatggaa actaataaaa aaactaaaaa
    12601 ggacggtatt taattatgaa aatcacagga caagcgcaat ttactaaaga aacaaatcaa
    12661 gaaaagtttt ataacggctc agcagggttt caagctggag aattcacagt gaaagttaaa
    12721 aatattgaat tcaatgatag agaaaataga tatttcacaa tcgtatttga aaatgatgaa
    12781 ggcaaacaat ataaacataa tcaatttgta ccgccgtata aatatgattt ccaagaaaaa
    12841 caattgattg aattagttac tcgattaggt attaagttaa atcttcctag cttagatttt
    12901 gataccaatg atcttattgg taagttttgt cacttggtat tgaaatggaa attcaatgaa
    12961 gatgaaggta agtattttac ggatttttca tttattaaac cttacaaaaa gggcgatgat
    13021 gttgttaaca aacctattcc aaagacagat aagcaaaaag ctgaagaaaa taacggggca
    13081 caacaacaaa catcaatgtc tcaacaaagc aatccatttg gaagcagtgg ccaatttgga
    13141 tatgacgacc aagatttagc gttttaaggt gtggtttaaa tgcaatacat tacaagatac
    13201 cagaaagaca atgacggcac ttattccgtc gttgctactg gtgttgaact tgaacaaagt
    13261 cacattgact tactagaaaa cggatatcca ctaaaagcag aagtagagat tccggataat
    13321 aaaaaactat ctatagaaca acgcaaaaaa atattcgcaa tgtgtagaga tatagaactt
    13381 cactggggag aaccagtgga atcaactaga aaattattac aaacagaatt ggaaattatg
    13441 aaaggttatg aagaaatcag tctgcgcgac tgttctatga aagttgcaag ggagttaata
    13501 gaactgatta tagcgtttat gtttcatcat caaataccta tgagtgtaga aacgagtaag
    13561 ttgttaagcg aagataaagc gttattatat tgggctacaa tcaaccgcaa ctgtgtaata
    13621 tgcggaaagc ctcacgctga cctagctcat tatgaagcag tcggcagagg aatgaacaga
    13681 aacaagatga atcactatga caaacatgta ttagcgttat gtcgcgaaca tcataacgaa
    13741 caacatgcga ttggcgttaa gtcgtttgat gataaatatc acttgcatga ctcgtggcta
    13801 aaagttgatg agaggcttaa taaaatgctg aaaggaagag aataatggtt aaatcgatat
    13861 ttttacaaga tggagaagaa atttttgttg atgatgaaga ttatgagaga gttaatcaat
    13921 atatttggac aaaatcttat gtagataacg ttagaagaat tcacacaaag acactcaacg
    13981 ttagcttaag tggatttgta ttagaaaatg gttttcaaaa aataaaaaat aatgatttta
    14041 ccaaaaacaa catcacttca attggttatc aacaacgatg ggcaaggcct acaagaaata
    14101 cttcgagtat ctataaaggt gtttatttaa atcgaaaaac aaaaaaatgg tctgctgtaa
    14161 taaaaattga tagcaaatct aaatatttag gtagttttgt taatgaatgg gaggcagcta
    14221 aagcatacaa cagcgcagta gataaatatt gggacggaca aggttataag aatcataaaa
    14281 atcaaaatga ctctatattt gaatatgaat acaaaactta caaagaccaa aaacgtcgta
    14341 gaagaggaaa aagtaagttc aaaggagtct atttaactca aagtggttat gtagcgcaaa
    14401 taacttataa aagaaagaca tatcatattg gatggtcaaa aaatatttat gagactgctc
    14461 tcatgtttaa taaaattaat ttttatttac atggttcaga cgtaatcctt aatgacgtac
    14521 ctatgacaga tgaacttaaa gaattcatat ctaactggga aataccggac aaaataaaag
    14581 cgctgaaagg agaagacaat gggagaagta tcgtggataa aacttaaagt tggcatgttt
    14641 gatgacagca aaatcaaata tatcgaagct ttacctgaaa gagatacgat cataactatt
    14701 tgggttaagt tgctaacttt atcaggaaag tacaacgaac aaggttacat tatgctatct
    14761 gaaaatttgc cgtacaacga agaaatgtta gcaaatgagt ttagccgacc tatcaactcg
    14821 ataaggttag caatacaaac ttttgagaca ttaggcatga ttgaaaaagt taacggtgtc
    14881 ataaaagtga caaactggga aaagcaccaa aacatcgaag gactcgagaa aatcagggcg
    14941 cagaacaggt tgaggaaaca aaagcaacga gaaaacaaca gaaaattgct aaatggtcac
    15001 gtgacgtcac gtgacagtca cgcaacagaa gaagataaag aattagataa agaattagaa
    15061 agagataaag aaaaagatat agataagaac ttaagttcaa ttaatagcgc aactgacgtt
    15121 acgcatgagc aatttgagga atggtggaaa ctttacgaca agaagaaaga taagaagatg
    15181 tcttttacta aattcaaatc atgcttaaag aaacattctt ttgaacaaat catgcaaggc
    15241 actcgagagt atttaaaaac tattacagac aaacaatatc aaaagtaccc caaaacattc
    15301 ttaactaatg aaagctatat gaatgattat agcgaagaga ttaaagaaac tggcatagat
    15361 caattggaac gtatgaagta cgacgaaagt tattgggatt agggggacat tatgaaacca
    15421 ctattcagcg aaaagataaa cgaaagcttg aaaaaatatc aacctactca tgtcgaaaag
    15481 ggattgaaat gtaagaggtg tggcagtgaa tacgacttat ataagttcgc tcctactaaa
    15541 aaacacccga atggttacga gtataaagat ggttgcaagt gtgaaattta tgaggaatat
    15601 aagcgaaaca agcaacggaa gataaacaac atattcaatc aatcaaatgt taatccgtca
    15661 ttaagagatg caacggttaa caactataag ccacaaaatg aaaaacaagt aaaagctaaa
    15721 caaacagcaa tagagtatgt acagggtttc tctacaaaag aaccaaaatc attaatattg
    15781 caaggttcat atggaactgg taaaagccac ctagcatacg ctatcgcaaa agcagtcaaa
    15841 tctaaagggc atacagttgc ttttatgcac ataccaatgt tgatggatcg tatcaaagcg
    15901 acatacaaca aaaatgcagt tgaaactaca gacgagctag tcagattgct aagtgatatt
    15961 gatttacttg tactagatga tatgggtgta gaaaacacag agcacacttt aaataaactt
    16021 ttcagcattg ttgataacag agtaggtaaa aacaacatct ttacaactaa ctttagtgat
    16081 aaagaactaa atcaaaatat gaactggcaa cgtatcaatt caagaatgaa acacaatgcg
    16141 agaaaagtaa gagtaatcgg agacgatttc agggagcgag atgcatggta accaaagaat
    16201 ttttaaaaac taaacttgag tgttcagata tgtacgctca gaaactcata gacgaggcac
    16261 agggcgatga aaataggttg tacgacctat ttatccaaaa acttgcagaa cgtcatacac
    16321 gccccgctat cgtcgaatat taaggagtgt taaaaatgcc gaaagaaaaa tattacttat
    16381 accgagaaga tggcacggaa gatatcaaag tcatcaagta taaagacaac gcaaatgaag
    16441 tttattcgct tacaggagcc catttcagcg acgaaaagaa aattatgact gatagtgacc
    16501 taaaacgatt caaaggcgct cacgggcttc tatatgagca agaactaggg ttacaagcaa
    16561 cgatatttga tatttagagg tggcacaatg agtaaataca acgctaagaa agttgagtac
    16621 aaaggaattg tatttgatag caaagtagag tgcgaatatt accaatattt agaaagtaat
    16681 atgaatggca ctaactatga tcgtatcgaa atacaaccga aatttgaatt acaacctaaa
    16741 ttcgggaaac aaagatcgat tacgtatata gccgatttct ctttgtggaa ggaagggaaa
    16801 ctggttgaag ttatagacgt taaaggtaag gcgactgaag ttgccaacat caaagcgaag
    16861 atattcagat atcagtatag agatgtgaat ttaacgtgga tatgtaaagc gcctaaatac
    16921 acaggtcaag aatggatggt atatgaggac ttagtgaaag tcagacgtaa aagaaaaaga
    16981 gaaatgaagt gatttaatgc aacaacaagc atatataaat gcaacgattg atataagaat
    17041 acctacagaa gttgaatatc atcatttcga tgatgtggat gatgaaaaag atatgctagc
    17101 aaagcgctta gatgacaatc cggatgaatt actaaagtat gacaacataa caataagaca
    17161 tgcatatata gaggtggaat aaatgagtat cgtaaaaatt aacggtaaac catataaatt
    17221 taccgaacat gaaaatgaat tgataaaaaa gaacggttta actccaggaa tggttgcgaa
    17281 aagagtacga ggtggctggg cgttgttaga agccttacat acaccttatg gtatgcgctt
    17341 agctgagtat aaagaaatcg tgttagccag aattatgcaa cgagaggcta gagaacgtga
    17401 aatagctagg caacgacgta aagaggctga gctaagaaga aagaagccac atttgtttaa
    17461 tgtaccacag aaacattcac gtgatccgta ctggtttgat aatacttata accaaatgtt
    17521 caagaagtgg caggaagtat aaatgcctaa aaccgataac gcacgcaaag aatacttaaa
    17581 ccaatttttc agatctaaga gatatctgta tcaggataac gagcgagtgg ctcatactca
    17641 tgtagtaaac ggcacttatt actttcatgg gcatatcgta ccagattggc aaggtgtgaa
    17701 aaagacattt gatacagcgg aagagctcgg aatatatata aagcaacatg gtttggaata
    17761 cgaggaacag aagcaactaa ctttatttta gaggagatgg aaacaatgaa aatcaaagtt
    17821 aaaaaagaaa tgctattaga cgagttaatt aaatgggcgc gagaaaatcc ggagctatca
    17881 caagggaaaa tatttttttc aacaggattt agtgatggat tcgttcgttt tcatccaaat
    17941 acaaataagt gttcgacgtc aagttttatt ccaattgata tccccttcat agttgatatt
    18001 gaaaaagaag taaccaaaga cacggtattt gataagttgt ttgaaatgta cgagatggaa
    18061 ggaggagtct atgaaaccgt attatatgca aacactagta taaaagaatg tttatatggt
    18121 agacgtttgc ctaccaaagc attctatatc ttaaacgatg atatgacgat gacgttaatt
    18181 tggaaagatg gggagttggt agtatgatgc cgaaatatcg agtgtgggac gaatatacag
    18241 gaagaataca cgatgttgta ggattcgact tcattgagac tgaagttcac tatgaaaact
    18301 acgcggaagc agaagcttta atacatgcaa gagattttaa agatgtagaa cttatgcaaa
    18361 gtacaggact taaagacaaa aacaacaacg aaatatatgc gggagatata gttgagtttg
    18421 aagatgaaat attagagatg ccagacgatg aatctgtaat aggaacaatt aatagagcag
    18481 taatatctat tgatgttgta aatggtattc aattaaaaga ttttatgttt gagggcgcag
    18541 tctccgaaaa tgattacttt gagtatatag acataaaatc cttccttaga tatgactgtg
    18601 aggttaaagg caacatattt gaatcatcac atttattgga ggtaacagaa tgaactatga
    18661 aacaggggtc caactaggtg taatggacgc taggttgaag aagatgagaa aacaacgtga
    18721 tgagtacaag aagcaacgag atgagcttat tggggatata gctaagttaa gagagcgtaa
    18781 cgaagagctg gagaacatgt ggcgcacagt caaaaatgaa ttgcttggaa gatacgaatt
    18841 ttaccgtttt agacttaacg aactacagat tgagagtaga gcgaacaagg cagtagctat
    18901 aaacatggga gctaaaatca acgcaagtgc tatattgtac cgaatggaca aattagacgg
    18961 aacaaatgag ttctacgaat ttttaggtca aatggaggaa gacactaatg aataaccgcg
    19021 aacaaatcga acagtccgtt ataagtacta gtgcgtataa cggtaatgac acagagggat
    19081 tgctaaaaga gattgaggac gtatataaga aagcgcaagc gtttgatgaa atacttgagg
    19141 gtttacctaa tgctatgcaa gatgcactca aagaagatat tggtcttgat gaagcagtag
    19201 ggattatgac ggggcaagtg gtctataaat atgaggagga gcaggaaaat gactaacaca
    19261 ttaacaattg atcagttaca agagttatta caaatacaaa aggactttga cgatagaata
    19321 ccaacactaa atttacgaga tagcaagatt gcgtatgtgg ttgaattctt tgaatggttt
    19381 aacacattgg aaacgttcaa gaattggaag aagaaaccag gtaaaccgtt agacgtacag
    19441 ctagacgagt tagcagacat gttagcgttt ggattgagta ttgctaatca acaagcagat
    19501 aacatggaag aaatttcggg ttatttagat gacggagatt ttaacgacta tatagaacga
    19561 gttgaaatcg attttaacga tagtgatgta gtagatgaat ttatgtcaac tatagatgaa
    19621 atgtatgaaa gtccatatag tagcaactta tttttaccgt ttgcattagc gaacaactac
    19681 tacactatcg atcaactcat tgacgcatac aaaaagaaaa tgaaaaggaa ccacgaaaga
    19741 caagatggaa cagcagacgc aggaaaagga tacgtgtaaa gacatcttag atcgagtcaa
    19801 ggaggttttg gggaagtgac acaatactta gtcacaacat tcaaagattc aacaggacgc
    19861 aagcatacac acataactaa agctaatagc aatcaaaggt ttacagttgt tgaggcagag
    19921 agtaaagaag aagcgaaaga gaagtacgag aaacaagtta aaagggatgc aattattaaa
    19981 gtgagtcagt tatttgaaaa tataaggaag tgtgggaaat gattaagcaa atactaagat
    20041 tattattctt actagcaatg tacgagttag gtaagtatgt aactgagcaa gtatatatta
    20101 tgatgacggc taatgatgat gtagaggcgc cgagtgactt cgcaaagttg agcgatcagt
    20161 ctgatttgat gagggcggag gtgtcagagt agatggactg gatatttttt tatactaaca
    20221 ttgttatatt cattgcatgt gtatatacaa tgtatagacg aattgaagtg agtaagaaaa
    20281 ttggtgaatt aagacgtgat ataaaagaga acgaaaaagc attggataat tataaaaaag
    20341 aaaacagacc aatcgaatat atcgtcgagt taaatgacgg tgtgtacttt cgaaaaaaac
    20401 atacagatgc gtttgcgcaa aggaccacat atattataac taataatatt ttcgaagcta
    20461 aatcatatga caatttatta tcagctaaaa tagatgccga aattctaaat ggccgtgtat
    20521 taaaatataa accaaattta gaggaggttg ggtagatgat gtggttcatc atagcaatta
    20581 tattattagt catcttattg tttggtgtga tgttgcaagc tgaacagtta aaaggtgaag
    20641 tgaaagttaa agagcgagag atagagatat taagaagtag attgagacat tttgaagatt
    20701 aacgggggtt aaacaaatga gtttgagaaa atcaacgcaa agatacttgg aaagcgaatt
    20761 aagtaattac aattacttcg ataaagatat agcgcgtgta agagatgaag ttttaaaccc
    20821 gtggagtcaa caagatacta atatcggtgg agatagggtt caaagtaatg taagtgtaac
    20881 cgaaataaaa gctattaggg tcgttaacga tagaagatta tcacaattag ctagaatgaa
    20941 gtcggctata gaggttgtgt ataatcacag cactgtagag actcaaaaac ttatggaact
    21001 ttattatttc aaaaaaccta gaacattaaa tctaactggt gtagctcaag aaataaatgt
    21061 aagtaaatct accgcttatg atatgaggaa agatatatta gttaggttag ctgatgaatt
    21121 aggtataata cattaagttt ggaaaaagtc tggaaaaata acgtcacttt cggtgttaat
    21181 atgatagcgt aagatattga ctatcttact gcgtttccct tatcgcaatt aggaataaag
    21241 gatctatgtg ggttggctga ttatagccaa tccctttttt aattttaaaa agcgtatagc
    21301 gcgagagttg gtggtaaatg aaatgaacga aaaacaaaag agattcgcag atgaatatat
    21361 aatgaatgga tgtaatggta aaaaagcagc aattacagca ggttatagta agaaaacagc
    21421 agagtcttta gcaagtcgat tgttaagaaa tgttaatgtt tcggaatata ttaaagaacg
    21481 attagaacag atacaagaag agcgtttaat gagtattaca gaagctttag cgttatctgc
    21541 ttctattgct agaggagaac ctcaagaggc ttacagtaag aaatatgacc atttaaacga
    21601 cgaagtggaa aaagaggtta cttacacaat cacgccaaca tttgaagagc gtcagagatc
    21661 tattgaccat atactaaaag ttcacggtgc gtatatcgat aaaaaagaaa ttactcagaa
    21721 gaatattgaa attaatattg gtgagtacga tgacgaaggt taaattaaat tttaagaaac
    21781 cgtctaatgt tttcaataga aacatattcg aaatactaac caattacgat aatttcactg
    21841 aagttcatta tggtggtggt tcaagtggta agtctcacgg cgttatacaa aaagttgtac
    21901 ttaaagcatt gcaagactgg aaatatccta ggcgtatact atggcttaga aaagtccaat
    21961 caacaattaa agatagttta ttcgaagatg tcaaagattg tttgataaac ttcggtattt
    22021 gggacatgtg cctttggaat aagactgata acaaagttga attgccaaac ggcgcagttt
    22081 ttttgtttaa aggattagat aacccagaga aaataaagtc gataaaaggc atatcagaca
    22141 tagtcatgga agaagcgtct gaattcacac taaatgatta cacgcaatta acgttgcgtt
    22201 tgagggagcg taaacacgtg aataagcaaa tatttttgat gtttaaccca gtatctaaac
    22261 tgaattgggt ttataagtat ttctttgaac atggtgaacc aatggaaaat gtcatgatta
    22321 gacaatctag ttatcgagat aataagtttc ttgatgaaat gacacgacaa aacttagagt
    22381 tgttagcaaa tcgtaatcca gcatattaca aaatttatgc gttaggtgaa tttgctacac
    22441 tagacaaatt ggttttccct aagtatgaaa aacgtttaat aaataaagat gagttaagac
    22501 atttaccttc ttattttgga ttggactttg gctacgttaa tgatcctagt gcttttatac
    22561 attctaaaat agatgtaaag aaaaagaagt tatacatcat tgaagagtat gttaaacaag
    22621 gtatgctgaa tgatgaaata gctaatgtca taaagcaact tggttatgct aaagaagaaa
    22681 ttacagcaga tagtgcagaa caaaaaagta tagctgaatt aaggaatcta gggcttaaaa
    22741 ggattttacc aaccaaaaaa gggaagggct cggttgtaca agggttacaa ttcttaatgc
    22801 aatttgaaat cattgttgat gaacgttgtt tcaagactat tgaagagttt gacaactaca
    22861 catggcaaaa ggacaaagat acaggtgaat ataccaatga accagtagat acatacaatc
    22921 attgtatcga ttcgttgcgt tattcagtgg aacgattcta cagaccggtt agaaaacgca
    22981 caaatgtcag ttcgaaagtt gacacaataa aatctctagg attataggag ggaacaaatg
    23041 ttaaaagtaa acgaatttga aacagataca gatctacggg gaaacataaa ttacttattt
    23101 aatgatgaag ccaatgttgt ttacacatat gacgggacgg aatccgattt attacaaaac
    23161 gttaatgaag taagtaaata cattgaacat cacatggatt accaacgacc tagattgaaa
    23221 gtgttaagtg attattacga aggtaaaact aagaacttag ttgagttaac acgacgcaaa
    23281 gaagagtaca tggcagataa ccgtgtagcg catgattacg catcttatat tagcgatttt
    23341 atcaacggct atttcttggg taatccaatt caatatcaag atgatgacaa agatgtatta
    23401 gaagctattg aggcgttcaa tgatttgaat gatgttgagt cacacaatag atctttagga
    23461 ttagatttgt caatttatgg taaagcttat gagttgatga ttagaaatca agatgatgaa
    23521 acgcgtttat acaagagtga tgcgatgagc acttttatca tatatgacaa cacagttgaa
    23581 cgtaacagta tcgcaggcgt tagatattta agaactaaac caatagacaa gaccgacgaa
    23641 gatgaagtgt ttactgttga tttattcact tcacacggtg tttatagata tcttaccaat
    23701 agaacaaatg gattgaagct cacaccacgt gaaaacagtt ttgaatctca ctcatttgaa
    23761 cgcatgccta ttacagaatt tagcaataac gaaagaagaa aaggggatta cgagaaagta
    23821 atcactttaa ttgatttgta tgataatgct gaatcagata cagctaacta tatgagtgat
    23881 ttaaatgacg ctatgttact tattaaaggt aatttaaatt tagatcctgt agaagttaga
    23941 aaacaaaagg aagctaacgt gttgttttta gaaccgactg tttatgctga tagcgaaggt
    24001 agagaaacag aaggttcagt tgacggtggt tatatttata agcaatacga tgtacaaggt
    24061 accgaagctt ataaagaccg tttgaacagt gatatacaca tgtttaccaa cacgcctaac
    24121 atgaaagatg ataactttag tggcactcaa tcgggcgagg caatgaaata taaattgttc
    24181 ggattagaac aacgtactaa aactaaagaa ggattgttca ctaaagggtt aagacgtcgt
    24241 gctaagttgt tagagacaat acttaaaaat acacggtcga ttgacgctaa caaagatttc
    24301 aatactgtta gatacgtata caacagaaac ttacctaaat cattgattga agaattaaaa
    24361 gcttatattg attctggcgg gaagattagc caaacaactt taatgtctct attctcgttc
    24421 ttccaagacc ctgaattaga agttaagaaa atcgaagaag atgagaaaga atctattaaa
    24481 aaagctcaaa aaggtattta taaagaccct agagacatca atgatgacga acaagatgat
    24541 gatacaaaag atactgttga taaaaaggaa tgattgtaat tgcctaacaa aaacactcaa
    24601 gaatattggg aagaacgcgg acgcaaagca atcgagaatg agttgaagcg tgataaaact
    24661 aaagctgaag aaatagaacg tatattgaat atgatgatta agcgcattga aaaagagatc
    24721 aatgcgttta ttgttaagta cggagatttt gcaggcgtta cattacaaga agcacaaaag
    24781 attattgatg agttcgatgt aaaagcgttt caagaagaag caaaaagatt ggtcgaaaac
    24841 aaggacttta gcgatagagc aaatgaagaa ttaaagaagt ataacactaa gatgtatgta
    24901 tctagagaac agatgttaaa gattcaaata gaattcttaa ttgcttatgc aacagctcaa
    24961 acagaattat cgatgaggga atatttcgaa tcaacagctt atcgtgtgtt cagtgatcaa
    25021 gcgggtattt taggtgaagg tgtacaagta gctaaagaag ttatagatac aatcgttgat
    25081 acacaatttc atggtgtcgt ttggtcagag cgattatgga ctaataccga agcaatgaaa
    25141 caagaagtag aagaaataat tgctaatgta gttattagag gtcgacatcc taatgaatat
    25201 gttaaagata tgcgcaagca cttaaataaa ttcgaaggca cagcacgaca aaagaccgca
    25261 gcaattaaat cattgcttta tacggaatcg gcacgtgttc acgcacaatc aagcattgac
    25321 agcatgaaag aaatttcacc ggaaggatat tatatgtata ttgcaaaaat cgataataga
    25381 acaactaaag tatgcaaagg gcttaatgga gaaatattca aagttaaaga cgctaaaatt
    25441 ggtgttaatt tctatcctat gcatatcaat tgtcgttcag attgcgcttt actacctaaa
    25501 tctatgtggc cgaaaaaacc aagcaagaaa cgaaaaacaa aatacttcgg agggaaagtg
    25561 aaaagcggtg attgatttaa aagtaaagct ttttaaaggc aagttagttt tgtatgacag
    25621 taaattaaat gtttggagga tactaatatg agtaatactg acaaatacct tagagacata
    25681 gcaagagaat taaaaggtat acgtaaagag ttacaaaagc gaaacgaaac agttattatt
    25741 gatgcaaact tagatagtgt aaggtcggca gtattagccg ataaagaaaa atcgaaatat
    25801 aatgaacctc tcttttaata gctagcactt aattgtgttg gctatttttt atgtccaaac
    25861 catgcttatg acaataaaag atgcaagtgt aacagcccga accatgtatg gcttaaaact
    25921 aatcaagagt aaataaatga ggtgtaaaaa ctatggatat ccaagagaag ttaaaactca
    25981 aattacagtt ttttgctgaa gaatcagatg gagataatgg aaaatcaaaa gataacaacg
    26041 atgatgaagg caaagacaaa caagacaaaa agactaattc agaagaagaa atcgaaaaaa
    26101 gactacaaga agaatataac aagcgtctta aagaagaatt aagtcgtcgt atgaagcaga
    26161 aagaaaaaga gaaacaagaa gctgttgatg aagctaaacg attagcaaaa atgaacaaag
    26221 atcaaatcgc tgaatatgaa cgcgaacaaa tggaaaaaga gctggaacaa ttacgttcag
    26281 aaaaacaatt aaacgaaatg cgttcagaag cacgaaaaat gttgagtgaa gcggaagttg
    26341 attcatcaga tgaggttgtc aatttggttg taactgacac tgctgaacaa actaaattga
    26401 atgttgaagc tttttctaat gcagtaaaaa aagcggttaa tgaagcggtt aagattaacg
    26461 ctagacaatc gccattgact ggtggagatt catttaatca ctcgactaaa aataaaccgc
    26521 aaaacttagc tgaaatagct agacaaaaaa gaattattaa aaattaacgg aggcatttaa
    26581 atggaacaaa cacaaaaatt aaaattaaat ttgcaacatt ttgcaagtaa caatgttaaa
    26641 ccaaaagtat ttaaccctga taatgtaatg atgcacgaaa agaaagatgg cacgttgatg
    26701 aatgaattca caacgcccat cttacaagag gttatggaaa actctaaaat tatgcaatta
    26761 ggtaagtacg aaccaatgga aggtactgag aagaagttta ctttttgggc tgataaacca
    26821 ggtgcttact gggtaggtga aggtcaaaaa atcgaaacat ctaaagctac atgggttaat
    26881 gctactatga gagcgtttaa attaggggtt atcttacctg taacaaaaga gtttttgaat
    26941 tacacttatt cacaattctt tgaagaaatg aagcctatga ttgttgaagc attctataaa
    27001 aagtttgatg aagcgggtat tttgaatcaa ggtaacaatc cattcggtaa atcaattgca
    27061 caatcaattg aaaaaactaa taaggttatt aaaggtgact tcacacaaga taacattatt
    27121 gatttagagg cattacttga agatgacgaa ttagaagcaa atgcgtttat ctcaaaaaca
    27181 caaaacagaa gcttgttacg taaaattgta gatcctgaaa caaaagaacg tatttatgac
    27241 cgtaacagtg atacgttaga tggtctacct gtggttaacc ttaaatcaag caacttaaaa
    27301 cgtggtgagt taatcactgg tgatttcgat aagttgattt acggtatccc tcaattaatt
    27361 gaatacaaaa tcgatgaaac tgcacaatta tctacagtca aaaatgaaga tggaacacct
    27421 gtaaacttgt ttgaacaaga catggtggca ttacgtgcaa ctatgcatgt agcattgcat
    27481 atcgctgatg ataaagcgtt tgctaagtta gttcctgctg atgcaaaacc atcttcaaat
    27541 ccaggagaag tttaataaat aattaggagt ggtaacatgc ccgaaatcat tggaattgtt
    27601 aaagtagatt ttacagattt agaagataac agacatgtct atatgaaagg gcatgtctac
    27661 cctcgcaaag gttatgatcc tacagatgaa cgtatcaaag ctttagctag tgttgaaaat
    27721 aaacgcaacg aacaaatgat ttacattgta aatgacaaat taaccaaaaa agaacttgtc
    27781 gaaatagcaa gtgttgctgg cttacaagtt gatgaaaaac aaacaaaagc tgaaattatc
    27841 aatgcttttg agtcactaga gtaggtggtt atatgactac gctagctgat gtaaaaaaac
    27901 gtattggtct taaagatgaa aagcaagatg aacaattaga ggaaattata aaaagttgtg
    27961 aaagccagtt gttatcaatg ttacctattg aagttgaaca aataccggaa aggtttagtt
    28021 acatgattaa agaagttgca gttaaacgct acaacaggat tggtgctgaa ggtatgacat
    28081 cagaagcggt tgacggacgt agcaatgcgt atgaattgaa cgatttcaag gagtatgaag
    28141 ctattattga taattacttt aatgctagaa cgagaactaa aaaaggaaag gctgtgttct
    28201 tttgagatat gaagatagag ctgtctttca atcagaacaa gcagcaactt acaatcctaa
    28261 aactagcaaa aaagaaaaca ctctaatcac ttatgatgcg ataccatgca atattaatcc
    28321 tatttctaga gcaagaaagc aacttgaatt cggcgatgta aaaaacgatg taagtgtcct
    28381 gaggataaaa gaatcaatat cttaccctgt tagccacgtg ttggttaatg gcattcgcta
    28441 caagatagtt gatacaagga tatacagaca cgaaacgtca tattatatcg aagaggtcaa
    28501 ttgatgaata tagatggatt agacgcactg ttaaatcaat ttcacgatat gaaaaccaac
    28561 atcgatgatg atgtagatga tactttacaa gaaaaagcca aagaatatgt agtacgagct
    28621 aaattgaaag ctagagaagt aatgaataag ggttattgga ctggtaattt atcacgcaat
    28681 atcagatata aaaaaactgg cgatttgcaa tacactatca catcgcatgc agcttatagt
    28741 ggtttcttag agtttggtac tcgatacatg gaggcagaac cttttatgtg gccagtatat
    28801 gaggtaataa gaaaatcaac tgtagaagaa ttgaaagcgt tgtttgaata ggagataaaa
    28861 gcatgacacc gaacttacaa ctttataata aagcttatga aacgctacaa ggatatggat
    28921 tccctgttat ttctcgtaaa gagatgcaac aagagattcc gtatcctttt tttgtaataa
    28981 aaatgccgga gtcaaacaga agtaaataca cgtttgatag ttattctggt gacacgaatt
    29041 tagttattga tatttggagt gtaagtgatg atttaggaca tcatgacgga cttgttaaaa
    29101 gatgtattga tgatttaaca cctagcgtta aaacaaacga ttatgacttt gaagaagatg
    29161 atactaacat cacacagtta gttgatgata ctaccaatca agaattgcta cacacatcag
    29221 taacgatatc ttacaaaaca ttttaaaaaa cggaggaata ttgaatggca aatatgaaaa
    29281 atagtaatga tcgtattatt ttatttagaa aagctggcga aaaagtagat gctactaaaa
    29341 tgcttttttt aactgaatac ggcttatcac atgaagctga tacagataca gaggatacga
    29401 tggatgggtc ttataacact ggtggttcag ttgaatcaac aatgtctggt actgctaaaa
    29461 tgttttatgg tgacgatttt gcagatgaaa ttgaagatgc agttgtagat cgcgtattgt
    29521 atgaggcttg ggaagttgaa agtagaatac caggcaaaaa tggagatgct actaaattta
    29581 aagcgaaata tttccaaggt ttccacaata aatttgaatt aaaagcagaa gctaacggta
    29641 ttgatgaata tgaatatgaa tatggagtga atggtcgttt ccaacgtgga tttgcaacac
    29701 tacctgaggc tgtaacaaag aaacttaagg cgactggata cagattccat gacactacaa
    29761 aagcagatgc gttaactggc gaagatttaa cagcaattcc acaacctaag gtagattcat
    29821 caacggttac accaggagag gtataaaaat agggcgttaa gccctattta ttttgtttaa
    29881 attaatcatg aatggagatt ttaagttatg aatgtagaaa ttaacggaaa gtcattagaa
    29941 ttaagttttg gttttaaatt tttaagagaa atcgataacc gattaggttt aaaagttgag
    30001 caagcttcta tcggtcaagg tgtatcaatg ttgcctgtag gtttagagag tggaaatcct
    30061 gttgtgattg gcgaagtttt aattgcagct acatctcact tgaaaaaaca agcaattact
    30121 attaataaca ttgatgaagc actagatgaa atcgcagaaa atattggact agaagaattt
    30181 ggttcggata ttttaacgga gttgggaaag cgacctatga cccgaaacct agtcgaagta
    30241 gtggaaacgg aagagaaacc agcgggagcc taataactta cgacagaatc gttataactt
    30301 gtatgtcaac acttggtatt acagatttga acgttattga gcaaatgaca ttaacagaat
    30361 ataactatcg aatgtatgcg aaagagtatg aaatgctaac ccaagaattc gaacgttaca
    30421 aacttgcgtt tgctattcgt gatgctgcag ctactaaaaa tgttgggaca gaaaataaac
    30481 ctaaagagga atatgttttt aacaacgcaa acgacgtatt gccttatgaa gaaaatatcc
    30541 aacggcttaa cgaaggtaaa gatataagat ttagtagcga acgtgatgaa tacgaaccac
    30601 aaaataatga attctttaaa gttatagcag aatttaataa gcaatagaaa gagaggtgtt
    30661 aatgtgacgg aatataaaat taaagcgact attgaagcta gtgtagccaa attcaaaagg
    30721 caaattgata gtgcggttaa ggcagtgcaa aaatacaagc gtacagctga taagactaaa
    30781 gatgttgaat taagtgctaa tgataaaaaa ttacaaaaaa ctatcaaggt tgctaagaag
    30841 actttagatt cgttcggtaa tgaaaaagca aaagctaaat tagatgctaa aatagaagat
    30901 ctgaaacaaa aagtattaga agcaagtttt gaattaaatc aattagattc aaaagaagtt
    30961 acaccagaag ttaagttaga aaaacaaaag ttaattaaag atatcattga aacagaagct
    31021 aagttatccg aactggaaaa gaaacgtgtc aatattgacg tcaatgctga taacagtaaa
    31081 tttaatcgag tgttaaaagt atctaaagct agtcttgaag cgttaaatag gtctaaagca
    31141 aaagctgtta tagacgtgga caatagtgtt gctaactcta aaatcaaacg tactaaagaa
    31201 gagcttaaga gtattccgaa caaaactaga tctcgactta atgtagatac agggctttct
    31261 ataccaacaa tctatgcatt taaaaaatcc ttagacgcat taccaaacaa aaaaacaacg
    31321 aaggtagatg tcgatactaa tggtttaaag aaagcttata cctacataat aaaagcaaac
    31381 gacaatttcc aaagacagat ggggaattta gctaatatgt tccgtgtgtt cggcactgta
    31441 ggttctaata tggttggtgg attattaact tcatctttta gtatcttaat acctgtaata
    31501 gcgagcgtag tacctgtagt gttcgcgcta ttaaacgcta tcaaagtatt aactggtggc
    31561 gtacttgctt taggtggtgc tgtagcaata gcgggagctg gatttgtagc gtttggcgca
    31621 atggctatca gcgctataaa aatgcttaac gacggaactt tacaagctag ttcggcaaca
    31681 aacgaataca aaaaagcttt agatggtgta aagtcagctt ggactgacat tataaaacaa
    31741 aatcaatcag ctatattcac atctttagca aatggtttaa acaccgttaa aacagcaatg
    31801 cagagcttac aaccgttttt cagcggtatt tcaagaggaa tggaagaggc gtctcaaagc
    31861 gtgcttaaat gggctcaaaa cagcggtgta gcatcaaggt tcttcaacat gatgaataca
    31921 actggtgttt cggtatttaa caaactatta agtgctgcag gcggtttcgg tgacggatta
    31981 gtcaatgtat tcacacaatt agcaccactg tttcaatggt cggctgattg gttggataga
    32041 ttaggtcaat ctttctctaa ctgggctaat agtgcagctg gagaaaattc gataactcga
    32101 tttattgaat acacaaaaac aaacttacct gtcattggta atatttttaa aaatgtgttc
    32161 gttggaatta ataatctaat gaatgcattc agcggatcat caactggaat ttttcaatct
    32221 cttgaacaaa tgacggctaa gtttagagaa tggtctgaac aagttggcca atctcaaggc
    32281 ttcaaagact ttgtcagtta tgtacaaaca aatggtccac taataatgca attaattgga
    32341 aacatcgcaa gaggattagt tgcattcgca acagcaatgg ctcctatagc tagtgcagta
    32401 ttacgcgttg cagtagcaat aactggttgg atagctaact tatttgaggc gcatccagct
    32461 acagcacaat tagtcggtgt catcatcact ttagttggtg catttaggtt tttaataccg
    32521 attattcttg ctgtatctaa ctttatgggt ggcggattaa taggtaggat cattgcatta
    32581 gtaagtaaat tcggtttatt aagagcggga ttaacaattt taaaaggtgc gttcatgtta
    32641 ttgaaaggac cattaaaaat tatatcagtt attttccaat tgttattcgg taagattgga
    32701 ttgattagaa atgctatcac aggactagta actgtttttg gtattctagg tggcccaata
    32761 acaatagtaa ttggtgtaat cgctgcatta atagctatat ttgttttatt gtggaacaaa
    32821 aacgaaggct tcagaaactt tattataaat gcttggaatg cgataaaaac gtttatggtt
    32881 aatgtttgga atgtattaaa agctgtagct tcggttgtat ggaatgctat tttaaaagct
    32941 atcactacag cagtaactaa tgtatacact tttataatga ttgtttggaa tcaaattgtc
    33001 gcatatttac aaggactctg gaacggtatt atcgctattg caacaacagt atggaacctt
    33061 ttagttacaa tcatcacaac tgttttcacg acgataatga caatagttat gacgatatgg
    33121 acagctattt ggacattctt aagtacaatc tggaacacga taattacaat cgctactacg
    33181 atttggaatt tgttagtcac tgtaataact actgtgttta caacaatcat gactatcgca
    33241 atgacaattt ggaatgctat ttggacattc ttaagtacaa tctggaacac gataattaca
    33301 atcgctacta cgatttggaa tttgttagtc actgtaataa ctactgtgtt tacaacaatc
    33361 atgactatcg caatgacaat ttggaatgct atttggacgt tcttacaaac gttgtggaac
    33421 actatagtta ctgtggcaac taaggtttgg aacgctatca ctacagctat atctgctgcg
    33481 ttacaagcgg catggagttt tatttctaat atatggaata cgatttggag tttcttatct
    33541 ggtatattaa cgacgatttg gaataaagtt gtaagtatat tcacacaagt tgtatcaact
    33601 atatcggaca aaatgtctca agcttggaac ttcatcgtga ctaaaggtat gcaatgggta
    33661 tctactataa caagtacgct aattaacttt gttaatagag ttattcaagg attcgttaat
    33721 gttgtaaaca aagttagtca aggtatgaca aatgcagtaa ataaaataaa aagctttata
    33781 ggagattttg tgtctgcagg tgctgatatg atccgtggtt taattagagg tattggacaa
    33841 atggctggtc aattagtaga tgcagctaaa aatgttgcta agaaagcttt agatgcagct
    33901 aaaagtgctt tgggtattca ctcaccttca cgtgaattca tggatgttgg tatgtattca
    33961 atgctaggtt tcgttaaagg tatagataat cattcaagta aagttatccg taatgtttct
    34021 aatgttgcag ataaagtagt tgatgcattt caacctacat taaacgcacc tgacatttct
    34081 agtattacag gaaacttaag taatttaggt ggaaatataa atgcgcaagt acaacacaca
    34141 cattctattg aaacatcacc gaacatgaaa actgttaaag ttgaattcga tgtcaataac
    34201 gatgcgctta ctagtattgt taacggcaga aatgctaaac gcaattctga gtattactta
    34261 taaaggaggt tacaaatgga catagaatta acaaaaaaag atggtactgt aatcaaatta
    34321 agtgaatacg ggtttatcgt taacgatata gtaattgata gcatgcaaat caacacaaag
    34381 tatcaagaca aagaaagtat gaacggtcgc atattaatgg ggagcaatta tatcagtaga
    34441 gatatagttg ttccttgttt ttgtaaagta aaaaatcgtt cagacattgc ttatatgcga
    34501 gatatgttgt attcgttaac tactgatata gaaccaatgt atttacgaga aatcagaaga
    34561 aaagaagagt tgaattacag gtttactcaa ccaacttctg atgattacgt gaaattagat
    34621 aaaaacaact tcccggatta tgaatattca agacacgatc aacaaattta tgtaaacggt
    34681 aaacagtata aagttatttt taacggagtt ataaacccta agcaaaaagg taataaagtt
    34741 tcttttgaac taaaattcga aactacagaa ttgccatacg gtgaaagtat tggaacaagc
    34801 ctagagttag aagaaaacaa aaaggttgga ttgtggtcgt ttgattttaa tatcgattgg
    34861 catgcaggcg gggataagcg ccagtataca tttgaaaatg ttagcaaaga tacagtttac
    34921 tatcatggta gcgctcctaa cgaccaattc aacatgtata aaaagataac aattattttg
    34981 ggcgaagata cagaattgtt tgtatggaat ttaacgcatg ctgaaataat gaaaatcgaa
    35041 gggatcaaac taaaagctgg agacagaatt gtttatgata gcttccgagt ttataaaaac
    35101 ggtgttgaaa taagtaccga aacgaatata gcccaaccaa aatttaaata cggagctaat
    35161 aaatttgagt ttaatcaaac ggtacaaaaa gttcagtttg atttgaaatt ttattataag
    35221 taggtgtcag aatgacaata actattaaac cacctaaagg taatggcgca cctgtaccag
    35281 tagaaacaac tttagtaaaa aaagttaatg ctgacggtgt attaactttt gatattcttg
    35341 aagataaata cacatacgaa gttattaacg caattggtaa aagatggatt gttagtcatg
    35401 tcgaaggtga aaacgacaag aaagaatatg taataactgt cattgatagg aaatcagaag
    35461 gcgacagaca actggttgaa tgtactgcta gagaaattcc tatagacaag ttaatgattg
    35521 ataggattta tgttaatgta acaggatctt ttacagtaga aagatatttt aacattgtgt
    35581 ttcaaggtac tggaatgctt tttgaagtcg aaggtaaggt taagtcttcg aagtttgaaa
    35641 atggtggtga aggcgataca aggttggaaa tgtttaaaaa gggattagaa catttcggtt
    35701 tagaatataa aataacgtat gacaaaaaga aaagcagata taagtttgta ttgacgcctt
    35761 ttgcaaatca aaaagcgtct tactttattt ctgacgaagt caacgccaac gctataaaac
    35821 ttgaagaaga cgcaagtaac ttcgccacct tcattagagg atacgggagt tattctggag
    35881 aagaaacata tgaacacgct ggacttgtta tggaggctag aagcgcatta gctgaaatat
    35941 acggtgatat acacgcagaa ccttttaaag atggcaaagt gactgatcaa gaaacaatgg
    36001 ataaagaatt acaatctaga ttaaaaaaat ctttaaaaca gtcactatct ttagattttt
    36061 tagttttaag agaagcctac cccgaagcag acccgcaacc tggtgacata gttcaaataa
    36121 agtctactat tttgggactt aacgacttag tacgtatagt agaaattaaa acgattagag
    36181 atataaataa tgtaattgta aagcaagatg taacgcttgg tgaatttaat cgagaacaac
    36241 gatatatgaa gaaagtaaat actgctgcta actatgtttc tggattaaat gacgttaacc
    36301 tttccaaccc tagtaaagcg gcagaaaact taaaatctaa agttgcatca atagctaaat
    36361 caacactcga tttaatgagt agaactgatt tgattgaaga taaacaacag aaggtaagct
    36421 ctaaaactgt aactacatct gacggcacta tcgttcatga ttttgtagat aaatcaaaca
    36481 ttaaagatgt aaaaacaatt ggaacaattg gcgattctgt agctagagga tcacatgcga
    36541 aagtgaattt tactgaaatg ttaggaaata agttgaacgc caaaataact aatcttgcta
    36601 aaggtggcgc cacgatggcg actgtgccta ttggtaagga tgcagtagag aatagtatat
    36661 atcgccaagc agaacaaata agaggcgacc taatcatatt acaaggtaca gatgatgact
    36721 ggttacacgg ttattgggca ggcgtaccga taggcactga taaaacggac actaaaacgt
    36781 tttacggtgc cttttgttct gcaattgaag ttatcaggaa aaataatcca gcttcaaaaa
    36841 tacttgtaat gacagctact aggcaatgcc ctatgagtgg tacaacgata cgccgtaaag
    36901 atacggacaa aaacaaacta gggttaactt tagaggatta tgtcaatgct cagatattgg
    36961 cttgtagtga attggatgta ccagtatatg atgcttatca cacagattat ttcaaaccat
    37021 ataatccagc atttagaaaa tctagcatgc ctgatggatt acatcctaat gaaagaggtc
    37081 atgaagttat tatgtatgag cttattaaaa attattatca gttttatgga tagtaaagga
    37141 ggaaaacatg agtaataaac taattacaga tttaagtaga gtctttgact acagatatgt
    37201 agatgaaaat gagtataact ttaaacttat ttcagacatg ctgacggatt ttaatttctc
    37261 tcttgaatac cacagaaata aagaggtatt cgcacatgat ggagaacaaa taaagtatga
    37321 acatttaaat gttacaagta acgtctctga ctttttaaca tatttaaacg gtcgatttag
    37381 caacatggta ctaggtcata acggcgacgg tatcaacgaa gtaaaagacg cgcgcgttga
    37441 taatacaggt tatggtcata agacattgca agatcgtttg tatcatgatt attcaacact
    37501 agatgttttc actaaaaagg ttgagaaagc tgtagatgaa cactataaag aatatcgagc
    37561 gacagaatac cgattcgaac caaaagagca agaaccggaa tttatcactg atttatcgcc
    37621 atatacaaat gcagtaatgc aatcattttg ggtagaccct agaacgaaaa ttatttatat
    37681 gacgcaagct cgtccaggta atcattacat gttatctaga ttgaagccca acggacaatt
    37741 tattgataga ttgcttgtta aaaacggcgg tcacggtaca cacaatgcgt atagatacat
    37801 tgatggagaa ttatggattt attcagctgt attggacagt aacaaaaaca acaagtttgt
    37861 acgtttccaa tatagaactg gagaaataac ttatggtaat gaaatgcaag atgtcatgcc
    37921 gaatatattt aacgacagat atacgtcagc gatttataat ccggtagaaa atttaatgat
    37981 ttttagacgt gaatataaac ccactgaaag acaacttaag aattcgttga actttgttga
    38041 ggttagaagt gctgacgata ttgataaagg tatagacaaa gtattgtatc aaatggatat
    38101 acctatggaa tacacttcag atacacaacc tatgcaaggt atcacttatg atgcaggtat
    38161 cttatattgg tatacaggtg attcgaatac agccaaccct aactacttac aaggcttcga
    38221 tatcaaaacg aaagaattgt tatttaaacg tcgtatcgat ataggcggtg tgaataacaa
    38281 ctttaaagga gatttccaag aggctgaggg tctagatatg tattacgatc tagaaacagg
    38341 acgtaaagca cttctaatcg gggtaactat tggacctggt aacaacagac atcattcaat
    38401 ttattctatc ggtcaaagag gtgtaaacca attcttgaaa aacatcgcac ctcaagtatc
    38461 aatgactgat tcaggcggac gtgttaaacc gttaccaata cagaacccag catatctaag
    38521 tgatattacg gaagttggtc attactatat ctatacgcaa gacacacaaa atgcattaga
    38581 tttcccgtta ccgaaagcgt ttagagatgc agggtggttc ttggatgtac tgcctggaca
    38641 ctataatggt gctctaagac aagtacttac cagaaacagc acaggtagaa atatgcttaa
    38701 attcgaacgt gtcattgaca ttttcaataa gaaaaacaac ggagcatgga atttctgccc
    38761 gcaaaacgcc ggttattggg aacatatccc taagagtatt acaaaattat cagatttaaa
    38821 aatcgttggt ttagatttct atatcactac tgaagaatca aaccgattta ctgattttcc
    38881 taaagacttt aaaggtattg caggttggat attagaagta aaatcgaata caccaggtaa
    38941 tacaacacaa gtattaagac gtaataactt cccgtctgca catcaatttt tagttagaaa
    39001 ctttggtact ggtggcgttg gtaaatggag tttattcgaa ggaaaggtgg ttgaataatg
    39061 gtagtagata atttttcgaa agatgataac ttaatcgagt tacaaacaac atcacaatat
    39121 aatccggtta ttgacacaaa catcagtttc tatgaatcag atagaggaac tggtgtttta
    39181 aattttgcag taactaagaa taacagaccc ttatctataa gttctgaaca tgttaaaaca
    39241 tctatcgtgt taaaaaccga tgattataac gtagatagag gcgcttatat ttcagacgaa
    39301 ttaacgatag tagacgcaat taatgggcgt ttgcagtatg tgataccgaa tgaattttta
    39361 aaacattcag gcaaggtgca tgctcaggca ttctttacac aaaacgggag taataatgtt
    39421 gttgttgaac gtcaatttag cttcaatatt gaaaatgatt tagttagtgg gtttgatggt
    39481 ataacaaagc ttgtttatat caaatctatt caagatacta tcgaagctgt cggtaaagac
    39541 tttaaccaat taaagcaaaa tatggctgat acacaaacgt taatagcaaa agtgaatgat
    39601 agtgcgacaa aaggcattca acaaatcgaa atcaagcaaa acgaagctat acaagctatt
    39661 actgcgacgc aaactagtgc aacacaagct gttacagctg aattcgataa aatagttgaa
    39721 aaagagcaag cgatttttga acgtgttaac gaagttgaac aacaaatcaa tggcgctgac
    39781 cttgttaaag gtaattcaac aacaaattgg caaaagtcta aacttacaga tgattacggt
    39841 aaagcaattg aatcgtatga gcagtccata gatagcgttt taagcgcagt taacacatct
    39901 aggattattc atattactaa tgcaacagat gcgccagaaa agacggatat aggcacgtta
    39961 gagaagcctg gacaagatgg tgttgatgac ggttcttcgt tcgatgaatc aacttataca
    40021 tcaagcaaat ctggtgtgtt agttgtttat gttgttgata ataatactgc tcgtgcaaca
    40081 tggtatccag atgattcaaa cgacgaatat acaaaatata aaattagtgg cacatggtac
    40141 ccgttttata aaaaaaatga cggcgattta actaagcaat tcgtcgaaga aacatcaaac
    40201 aacgctttaa atcaagccaa gcagtatgta gataataaat tcggaacaac gagttggcaa
    40261 caacataagt taactgagcc taacggccaa tcaatacaag ttaacttgaa taatgcacaa
    40321 ggcgatttgg gttatctaac tgcaggtaat tactatgcaa caagagtgcc ggatttacca
    40381 ggtagcgttg aaagttatga gggttattta tcggtattcg ttaaagatga tacaaacaag
    40441 ctatttaact tcacgcctta taactctaaa aagatttaca cacgatcaat cacaaacggc
    40501 agacttgagc aacagtggac agttcctaat gaacataaat caacggtatt gttcgacggt
    40561 ggcgcaaatg gtgtaggtac aacaatcaat ctaactgaac cgtacacaaa ctattctatt
    40621 ttgttggtaa gtggaactta tccaggtggc gttattgagg gattcggact aaccgcatta
    40681 cctaatgcaa ttcaattaag caaagcgaat gtagttgact cagacggtaa cggtggcggt
    40741 atttatgagt gtttactatc aaaaacaagt agtaccactt taagaataga caacgatgtg
    40801 tatttcgatt taggcaaaac atcaggttct ggaacgaatg ccaacaaagt tactataact
    40861 aaaattatgg ggtggaaata atgaaaataa cagtaaacga taaaaacgaa gttatcggat
    40921 acgttaatac tggcggttta cgcaatagtt tagatgtaga tgataacaat gtgcctatca
    40981 aattcaaaga agagtttgaa cctagaaagt ttgttttcac aaacggcgaa attaaatata
    41041 acagcaattt tgaaaaagaa gacgtaccga atgcatcaag ccaacaaagt gaatcagatt
    41101 tgagtgatga agaacttcgc ggaatggttg cgagtatgca aatgcaggtg gcacaagtaa
    41161 acgtattaac aatggaatta gctcaacaaa acgctatgtt aacacaacag ttgactgaac
    41221 tgaaaactaa caaaacaagt actgaggggg acgtttaatg atgaagatga tttatccgac
    41281 ttttaaagac atcaaaactt tttatgtttg gggttactat aaaaacgagc aaattaagtg
    41341 gtacgtagac aagggtttaa tcgataaaga agaatacgct ttaatcactg gagaaaaata
    41401 tccagaaaca aaagatgaaa agtcacaggt gtaatgcttg tggcttttta atttaacaaa
    41461 aagtaggtgg cgtaatgttt ggttttacca aacgacatga acaagattgg cgtttaacgc
    41521 gattagaaga aaatgataag actatgtttg aaaaattcga cagaatagaa gatagtctta
    41581 gagcgcaaga aaagatttat gacaaattag atagaaattt tgaagaatta aagcgcgaca
    41641 aggtagaaga tgaaaagaat aaagaaaaga atgccaagaa tattagagac ataaaaatgt
    41701 ggattctcgg tttgataggg actatcttca gtacgattgt cgtagcttta ctaagaacta
    41761 tttttggcat ttaaaggagg tgattaccat gcttaaaggg attttaggat atagcttctg
    41821 ggcgtgcttc tggtttggta aatgtaaata acagttaaga gtcagtgctt cggcactggc
    41881 tttttatttt gattgaaatg aggtgcatac atgggattac ctaatccaaa gactcgaaaa
    41941 cctacagcta gtgaagtagt agagtgggcg ttgtatattg ctaaaaagaa aatagctatt
    42001 gatgtacccg gttctggaat gggggcgcaa tgttgggact tgcctaatta cttgcttgat
    42061 aaatattggg ggtttagaac atggggaaat gctgatgcta tggctcagaa atctaattat
    42121 agaggtagag atttcaagat aattagaaat acaaaagatt ttgtaccaca accaggcgac
    42181 tggggtgttt ggactggtgg ttgggcagga catgtaaaca ttgtagtggg accatgcaca
    42241 aaagactatt ggtatggcgt agatcaaaac tggtatacaa ataacgcaac aggaagtccg
    42301 ccatataaaa ttaagcactc ttatcatgat ggaccaggtg gaggagttaa atattttgtt
    42361 agaccaccat atcatccgga gaaatctacg ccggcaccta aacccgaaga tgatagtgat
    42421 gataacgaaa aaaataataa aaaagttcca atttggaaag atgtaacaac tataaagtac
    42481 acaatttcta gccaagaagt taattatcca gaatatattt atcactttat agtagagggt
    42541 aatcgacgac tcgaaaaacc taaagggata atgattagaa acgctcaaac aatgagctcg
    42601 gtagaaagtt tatataacag tagaaagaaa tataagcaag atgtagaata tccacacttt
    42661 tatgttgata gacataatat ttgggctcct agaagagccg tatttgaagt tcctaatgaa
    42721 gctgattata tagttataga cgtatgtgaa gattatagtg cgagtaaaaa cgaatttatt
    42781 tttaacgaga ttcacgcaat ggttgtagct gtagatatga tgatcaaata tgagatacct
    42841 ctaagtattg agaatttaaa agtagacgat agtatttggc gttcaatgtt ggaacatgtt
    42901 aattggaata tgattgacaa cggtgttccc cctaaagata aatacgaagc attagaaaag
    42961 gcattactta atatatttaa aaacagagaa aaattattaa attctataac taaaccaaca
    43021 gtaacaaaat ctagaataaa agttatggta gataataaaa acgctgatat agcgaatgta
    43081 agagactcat caccaacagc taataatggc tcggcatcta aacaaccgca gatcataaca
    43141 gaaacgagtc cttatacatt caaacaagca ctggataaac aaatggcaag aggtaacccg
    43201 aaaaaatcta atgcttgggg ttgggctaac gctacacgag cacaaacgag ctcggcaatg
    43261 aatgttaaac gaatatggga aagtaacaca cagtgctacc aaatgcttaa tttaggcaag
    43321 tatcaaggtg tttcagttag cgcacttaat aagatactta aaggtaaggg aacattgaat
    43381 aatcaaggta aagcgttcgc agaagcttgt aaaaaacaca acattaatga aatttattta
    43441 atcgcgcatg ctttcttaga aagtggatat ggaacaagta acttcgctaa cggaaaagat
    43501 ggagtataca actacttcgg cattggcgct tacgacaaca atcctaacta cgcaatgacg
    43561 tttgcaagga ataaaggttg gacatctcca gcaaaagcaa tcatgggcgg tgctagcttc
    43621 gtaagaaagg attacatcaa taaaggtcaa aacacattgt accgaattag atggaatcct
    43681 aagaatccag ctacccacca atacgctact gctatagagt ggtgccaaca tcaagcaagt
    43741 acaatcgcta agttatataa acaaatcggc ttaaaaggta tctacttcac aagggataaa
    43801 tataaataaa gaggtgtgta aatgtacaaa ataaaagatg ttgaaacgag aataaaaaat
    43861 gatggtgttg acttaggtga cattggctgt cgattttaca ctgaagatga aaatacagca
    43921 tctataagaa taggtatcaa tgacaaacaa ggtcgtatcg atctaaaagc acatggctta
    43981 acacctagat tacatttgtt tatggaagat ggctctatat tcaaaaatga gccccttatt
    44041 atcgacgatg ttgtaaaagg gttccttacc tacaagatac ctaaaaaggt tatcaaacac
    44101 gctggttatg ttcgctgtaa gctgttttta gagaaagaag aagaaaaaat acatgtcgca
    44161 aacttttctt tcaatatcgt tgatagtggt attgaatctg ctgtagcaaa agaaatcgat
    44221 gttaaattgg tagatgatgc tattacgaga attttaaaag ataacgcgac agatttattg
    44281 agcaaagact ttaaagagaa aatagataaa gatgtcattt cttacatcga aaagaatgaa
    44341 agtagattta aaggtgcgaa aggtgataaa ggcgaaccgg gacaacctgg tgcgaaaggt
    44401 gatacaggta aaaagggaga acaaggcaca cccggtaaaa acggtactgt agtatcaatc
    44461 aatcctgaca ctaaaatgtg gcaaattgat ggtaaagata cagatatcaa agcagaacct
    44521 gagttattgg acaaaatcaa tatcgcaaat gttgaagggt tagaaaataa attgcaagaa
    44581 gttgaaaaaa tcaaagatac aactctcaac gactctaaaa cgtatacgga ttcaaaaatt
    44641 gctgaactag ttgatagcgc gcctgaatct atgaatacat taagagaatt agcagaagca
    44701 atacaaaata actctatttc agaaagtgta ttgcaacaga ttggctcaaa agttagtaca
    44761 gaagattttg agagattcaa gcaatcatta aacagtttgt atgcagataa aaatcatagt
    44821 catacaatca aacagattga aggattagaa aatgctttat caaaaaaatc agacataaat
    44881 cacagtcatg atgaacgtta tcttttatca tcaaatgctt ttacaaaaga ggaagcagat
    44941 aaactttatc aacctatcgg ttcttcgcag ccgtcactga atatttggac aggcagtgaa
    45001 acagaatata attatttgta tcaaaaagac cctaatacac tttacttaat taaggggtga
    45061 ttttatggaa ggtaatttta aaaatgtaaa gaagcttatt tacgaaggcg aagaatatac
    45121 aaaagtatat gctggaaata tccaagtatg gaaaaagcct tcatcttttg taataaaacc
    45181 cttacctaaa aataaatatc cggatagcat agaagaatca acagcaaaat ggacaataaa
    45241 cggagttgaa cctaataaaa gttatcaggt gacaatagaa aatgtacgta gcggtataat
    45301 gagggtttcg caaactaatt taggttcaag tgatttagga atatcaggag tcaatagcgg
    45361 agttgcaagt aaaaatatca actttagtaa tccttcaggg atgttgtatg taactataag
    45421 tgatgtttat tcaggatctc caacattgac cattgaataa ttttaaacga ctaatttttt
    45481 agtcgttttt tattttgg
//
LOCUS       DAKTIP010000004        38357 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAKTIP010000004 DAKTIP010000000
VERSION     DAKTIP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 38357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.004"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..555)
                     /locus_tag="O0305_000166"
     CDS             complement(<1..555)
                     /locus_tag="O0305_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0273353.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNE"
     gene            622..1092
                     /locus_tag="O0305_000167"
     CDS             622..1092
                     /locus_tag="O0305_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCY0273354.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            1239..2108
                     /locus_tag="O0305_000168"
     CDS             1239..2108
                     /locus_tag="O0305_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0273355.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMSIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            2129..2695
                     /locus_tag="O0305_000169"
     CDS             2129..2695
                     /locus_tag="O0305_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0273356.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            3126..5969
                     /gene="sdrC"
                     /locus_tag="O0305_000170"
     CDS             3126..5969
                     /gene="sdrC"
                     /locus_tag="O0305_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCY0273357.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTAPSENKTTKKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTKTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHVNQTTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDIFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVRGSFEQVAFAKRKNATTDKTAYKMEVTLGNDTYSEEIIVD
                     YGNKKAQPLISSTNYINNEDLSRNMTAYVNQPKNTYTKQTFVTNLTGYKFNPNAKNFK
                     IYEVTDQNQFVDSFTPDTSKLKDVTDQFDVIYSNDNKTATVDLMKGQTSSNKQYIIQQ
                     VAYPDNSSTDNGKIDYTLDTDKTKYSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
     gene            6336..10481
                     /gene="sdrD"
                     /locus_tag="O0305_000171"
     CDS             6336..10481
                     /gene="sdrD"
                     /locus_tag="O0305_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCY0273358.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVVNEKNSIGSAFTETVSHVGNKENPGYYKQTIYVNPSENSLTNAKLKVQAYHSS
                     YPNNIGQINKDVTDIKIYQVPKGYTLNKGYDVNTKELTDVTNQYLQKITYGDNNSAVI
                     DFGNADSAYVVMVNTKFQYTNSESPTLVQMATLSSTGNKSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGVQELGEKGVGNVTVTVFDNNTNTKVGEAVTKEDGSYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTKTDENGKYCFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDRDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDRDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DAGKHTPVKPMSTTKDHHNKAKALPETGNENSGSNNATLFGGLFAALGSLLLFGRRKK
                     QNK"
     gene            10875..14339
                     /gene="sdrE"
                     /locus_tag="O0305_000172"
     CDS             10875..14339
                     /gene="sdrE"
                     /locus_tag="O0305_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrE"
                     /protein_id="HCY0273359.1"
                     /translation="MINRDNKKAITKKGMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAENTSTENAKQDDATTSDNKEVVSETENNSTTENNSTNPIKKETNTDSQP
                     EAKKESTSSSTQKQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPV
                     NVSKEELKNNPEKLKELVRNDSNTDHSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVA
                     SNNVNDLIKVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKN
                     VIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKSRLTLYSY
                     IDKKTVPNETSLNLTFATAGKETSQNVTVDYQDPMVHGDSNIQSIFTKLDEDKQTIEQ
                     QIYVNPLKKSATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNSDQQLPQS
                     NRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQ
                     GTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQ
                     GTDSKEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPT
                     KVNGTTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDAN
                     EPGIKDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTT
                     ADDKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKD
                     VTVTLQNEKGEVIGTTKTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDA
                     DGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAALGSLLLF
                     GRRKKQNK"
     gene            14458..15930
                     /locus_tag="O0305_000173"
     CDS             14458..15930
                     /locus_tag="O0305_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0273360.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(16054..17544)
                     /locus_tag="O0305_000174"
     CDS             complement(16054..17544)
                     /locus_tag="O0305_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0273361.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(17826..18704)
                     /locus_tag="O0305_000175"
     CDS             complement(17826..18704)
                     /locus_tag="O0305_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCY0273362.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            complement(18717..19382)
                     /gene="bshB2"
                     /locus_tag="O0305_000176"
     CDS             complement(18717..19382)
                     /gene="bshB2"
                     /locus_tag="O0305_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCY0273363.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLANYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            complement(19396..19758)
                     /locus_tag="O0305_000177"
     CDS             complement(19396..19758)
                     /locus_tag="O0305_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCY0273364.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            20034..20792
                     /gene="nagB"
                     /locus_tag="O0305_000178"
     CDS             20034..20792
                     /gene="nagB"
                     /locus_tag="O0305_000178"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCY0273365.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            20869..21501
                     /gene="hxlA"
                     /locus_tag="O0305_000179"
     CDS             20869..21501
                     /gene="hxlA"
                     /locus_tag="O0305_000179"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCY0273366.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            21503..22051
                     /gene="hxlB"
                     /locus_tag="O0305_000180"
     CDS             21503..22051
                     /gene="hxlB"
                     /locus_tag="O0305_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCY0273367.1"
                     /translation="MAKFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            22164..22811
                     /locus_tag="O0305_000181"
     CDS             22164..22811
                     /locus_tag="O0305_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0273368.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEAALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQRAGMQSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            23313..24713
                     /locus_tag="O0305_000182"
     CDS             23313..24713
                     /locus_tag="O0305_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273369.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            25257..26633
                     /locus_tag="O0305_000183"
     CDS             25257..26633
                     /locus_tag="O0305_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0273370.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            26635..27774
                     /locus_tag="O0305_000184"
     CDS             26635..27774
                     /locus_tag="O0305_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCY0273371.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            27749..28114
                     /locus_tag="O0305_000185"
     CDS             27749..28114
                     /locus_tag="O0305_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCY0273372.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            28117..28386
                     /locus_tag="O0305_000186"
     CDS             28117..28386
                     /locus_tag="O0305_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273373.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(28587..28754)
                     /locus_tag="O0305_000187"
     CDS             complement(28587..28754)
                     /locus_tag="O0305_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCY0273374.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(29261..30091)
                     /gene="thiD"
                     /locus_tag="O0305_000188"
     CDS             complement(29261..30091)
                     /gene="thiD"
                     /locus_tag="O0305_000188"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCY0273375.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            30275..30931
                     /locus_tag="O0305_000189"
     CDS             30275..30931
                     /locus_tag="O0305_000189"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCY0273376.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            30932..31312
                     /locus_tag="O0305_000190"
     CDS             30932..31312
                     /locus_tag="O0305_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCY0273377.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTVAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            31445..31813
                     /locus_tag="O0305_000191"
     CDS             31445..31813
                     /locus_tag="O0305_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCY0273378.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            31934..33418
                     /locus_tag="O0305_000192"
     CDS             31934..33418
                     /locus_tag="O0305_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0273379.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLFVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(33560..34012)
                     /locus_tag="O0305_000193"
     CDS             complement(33560..34012)
                     /locus_tag="O0305_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0273380.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(34025..34789)
                     /locus_tag="O0305_000194"
     CDS             complement(34025..34789)
                     /locus_tag="O0305_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0273381.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            complement(35338..36090)
                     /locus_tag="O0305_000195"
     CDS             complement(35338..36090)
                     /locus_tag="O0305_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCY0273382.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRIVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAIS"
     gene            36258..37244
                     /gene="pta"
                     /locus_tag="O0305_000196"
     CDS             36258..37244
                     /gene="pta"
                     /locus_tag="O0305_000196"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCY0273383.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            37247..38083
                     /locus_tag="O0305_000197"
     CDS             37247..38083
                     /locus_tag="O0305_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0273384.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
BASE COUNT        14046 a         5835 c         6758 g        11718 t
ORIGIN      
        1 ttcattttta agaaaatcaa tagaagttgt atcgattaaa actacatttg aaccattact
       61 ttgtaaggac tcataatact cacgataatc tttttttaac tttaacagat attcatcttc
      121 tatttgatgc tcaaaactac ggttacgttt agcaattcta gattttaaca catcaaggtc
      181 tgcatctaaa aagataatca tattcggcat aatcatatct tcagttaaaa tatcataaat
      241 tttactgaat ttctgaaatt caacagaact caaagtattt ttagcaaata tcttattttt
      301 atgtatatga taatcactaa ctacaccttg atttagttgt gttacatctt gaaattgctt
      361 atatctattg cataaaaaga acatttcagt ttgaaaactc catttagaga tatcttcata
      421 aaagtctgat aaaaatggat tttctgtgat gatttctttt tcttcataaa aatctaaagt
      481 ttgacttaat ttgtgtgcaa gtgaagattt acctacgcca ataggacctt caattgctat
      541 aaaaggtttg ttcatattca catctccaaa tgttaaaata gacattgagt attgtaccat
      601 aagtgaggta tctggacata aatgacaaat gatatatatt ttatgacatt agcgattgaa
      661 gaagctaaaa aagcagctca actaggcgaa gtacctatag gtgctatcat cactaaagat
      721 gatgaagtta tcgctagagc acataattta agagaaacac tacaacaacc aacggcgcat
      781 gctgaacata ttgcaattga acgtgcagcc aaagtgttag gtagttggcg tttagaaggt
      841 tgcacattat atgtaacctt agaaccatgt gtcatgtgcg caggaacaat tgtaatgagt
      901 cgcattccaa gagtcgtcta tggcgcagat gatcctaaag gtggttgtag tggcagttta
      961 atgaatttat tgcaacaatc taattttaat catcgtgcaa ttgttgataa aggtgtactt
     1021 aaagaagcat gtagcacatt attaacaaca ttttttaaaa acttaagagc caataagaaa
     1081 tccaccaatt agacgatagt acatactaat taaaatttgt tagaattaca ttatatgata
     1141 aattaatgac aatataaatg ttaatagaaa agtatgtgcg ctgagtatat attcttattt
     1201 aagaacaaga ttttaaattt acgaaacgag gtaacataat gataaaacta atagccactg
     1261 atatggatgg cacgctactt aatgcagcac atgaaatttc tcaacctaat attgatgcga
     1321 ttaaatacgc tcaagaacaa gggataacgg ttgttatcgc gacaggtcga gcattttatg
     1381 aagcacaagc accagttgct gacacagatt taacagtacc atatatttgt ttgaatggtg
     1441 ctgaagtacg tgatgaaact ttcaatgtaa tgagcacttc acaccttaat aaatcgttag
     1501 tacacaaaat tacaaatgtt ttaaaagatg caggtattta ttatcaagta tacacgagtc
     1561 gtgcgattta tactgaagat ccacaaagag atttagacat ttacatagat attgctgagc
     1621 gtgcaggtca acatgcaaac gttgagcgta ttaaaaatgg tattcaaaga cgcatagata
     1681 atggtacgtt gaaagttgtt gataattatg atgctattga aaacatacct ggtgaattaa
     1741 ttatgaaaat attagcattt gatggaaatt tagaaaaaat tgacaaagct agtaaaattt
     1801 tagctgaatc tccgaattta gctatatcat catcttcgag aggaaatata gaaataacgc
     1861 attcagatgc acaaaaaggt attgcgctag aaacaattgc cgaaagatta gggattgaaa
     1921 tgaaagaagt catgtcaata ggtgacaatt taaatgactt atcaatgtta gagaaagttg
     1981 gctatccagt tgcgatggaa aatggtgcag aagaagttaa aaaaatagcg aaatatgtca
     2041 cagatacgaa tgaaaatagt ggtgttggaa aagctattat gaaattatta cgtgaacaac
     2101 aagtttaata aaaaaagagg ggtcaaatat gaaaggatta attattattg gcagtgcaca
     2161 agtgaattca catacaagtg cactagcaag atacttaact gagcatttta aaacacatga
     2221 tattgaagcg gaaatattcg atttagcaga aaaaccgtta aatcaattag atttttcagg
     2281 aacaacaccg tctattgatg aaatcaaaca aaatatgaaa gatttaaaag agaaagcaat
     2341 ggcggcggac tttttaatat taggaacgcc aaactatcat ggttcatatt ctggaatatt
     2401 gaaaaatgca ttagatcatc taaatatgga ttattttaaa atgaaacctg taggcttaat
     2461 aggaaatagt ggtggtattg ttagttcaga gccattgtca catttaagag taatcgtcag
     2521 aagtttacta ggcattgctg taccaactca aatagcaaca catgattctg attttgctaa
     2581 aaatgaagat ggttcatatt acttaaatga tagtgaattc caattacgag caagattatt
     2641 tgtcgatcaa attgtatctt ttgtgaataa tagtccatat gaacatttaa aataatatta
     2701 aaaaatgtgt aaatatacat taaaaatagg tatgtagctt aaggtatttc attgagaatg
     2761 cttttaagtc acatgcctat tttttgttct aatgcaccaa tagtaagcct ttgatattac
     2821 tgttgtgagc aatgttatta attaaaataa gatgtaatag cgaattgaaa acaagcttag
     2881 attaaaagtg agttttaaaa atatagataa ataatttaaa gcaattaaag aaaaaaagta
     2941 ttaaaaattg caattcttaa actgtgattc atattaatat ttctagcaaa taacattgta
     3001 aaataaagaa aaataattaa agtattgcac tttattgaaa tttatattac gatagtaatg
     3061 cagaaattta tatatgcaaa atattatatt tatcaaattt tgatatttta aaggagtatt
     3121 attaaatgaa taataaaaag acagcaacaa atagaaaagg catgatacca aatcgattaa
     3181 acaaattttc gataagaaag tattctgtag gtactgcttc aattttagta gggacaacat
     3241 tgatttttgg gttaagtggt catgaagcta aagcggcaga acatacgaat ggagaattaa
     3301 atcaatcaaa aaatgaaacg acagccccaa gtgagaataa aacaactaaa aaagttgata
     3361 gtcgtcaact aaaagacaat acgcaaactg caactgcaga tcagcctaaa gtgacaatga
     3421 gtgatagtgc aacagttaaa gaaactagta gtaacatgca atcaccacaa aacgctacag
     3481 ctaatcaatc tactacaaaa actagcaatg taacaacaaa tgataaatca tcaactacat
     3541 atagtaatga aactgataaa agtaatttaa cacaagcaaa agatgtttca actacaccta
     3601 aaacaacgac tattaaacca agaactttaa atcgcatggc agtgaatact gttgcagctc
     3661 cacaacaagg aacaaatgtt aatgataaag tacatttttc aaatattgac attgcgattg
     3721 ataaaggaca tgttaatcag actactggta aaactgaatt ttgggcaact tcaagtgatg
     3781 ttttaaaatt aaaagcaaat tacacaatcg atgattctgt taaagagggc gatatattta
     3841 cttttaaata tggtcaatat ttccgtccag gatcagtaag attaccttca caaactcaaa
     3901 atttatataa tgcccaaggt aatattattg caaaaggtat ttatgatagt acaacaaaca
     3961 caacaacata tacttttacg aactatgtag atcaatatac aaatgttaga ggtagctttg
     4021 aacaagttgc atttgcgaaa cgtaaaaatg caacaactga taaaacagct tataaaatgg
     4081 aagtaacttt aggtaatgat acatatagcg aagaaatcat tgtcgattat ggtaataaaa
     4141 aagcacaacc gcttatttca agtacaaact atattaacaa tgaagattta tcgcgtaata
     4201 tgactgcata tgtaaatcaa cctaaaaata catatactaa acaaacgttt gttactaatt
     4261 taactggata taaatttaat ccaaatgcaa aaaacttcaa aatttacgaa gtgacagatc
     4321 aaaatcaatt tgtggatagt ttcacccctg atacttcaaa acttaaagat gttactgatc
     4381 aattcgatgt tatttatagt aatgataata aaacagctac agtcgattta atgaaaggcc
     4441 aaacaagcag caataaacaa tacatcattc aacaagttgc ttatccagat aatagttcaa
     4501 cagataatgg aaaaattgat tatactttag acactgacaa aactaaatat agttggtcaa
     4561 atagttattc aaatgtgaat ggctcatcaa ctgctaatgg cgaccaaaag aaatataatc
     4621 taggtgacta tgtatgggaa gatacaaata aagatggtaa acaagatgcc aatgaaaaag
     4681 ggattaaagg tgtttatgtc attcttaaag atagtaacgg taaagaatta gatcgtacga
     4741 caacagatga aaatggtaaa tatcagttca ctggtttaag caatggaact tatagtgtag
     4801 agttttcaac accagccggt tatacaccga caactgcaaa tgtaggtaca gatgatgctg
     4861 tagattctga tggactaact acaacaggtg tcattaaaga cgctgacaac atgacattag
     4921 atagtggatt ctacaaaaca ccaaaatata gtttaggtga ttatgtttgg tacgacagta
     4981 ataaagatgg taaacaagat tcgactgaaa aaggaattaa aggtgttaaa gttactttgc
     5041 aaaacgaaaa aggcgaagta attggtacaa ctgaaacaga tgaaaatggt aaataccgct
     5101 ttgataattt agatagtggt aaatacaaag ttatctttga aaaacctgct ggcttaactc
     5161 aaacaggtac aaatacaact gaagatgata aagatgccga tggtggcgaa gttgatgtaa
     5221 caattacgga tcatgatgat ttcacacttg ataatggcta ctacgaagaa gaaacatcag
     5281 atagcgactc agattctgac agcgattcag actcagatag cgactcagat tcagatagcg
     5341 actcagattc agacagcgat tcagacagcg actcagactc agatagcgat tcagattcag
     5401 acagcgactc agactcagac agcgattcag actcggatag cgactcagac tcagatagcg
     5461 actcagattc ggatagcgac tcagactcag atagcgattc agattcagat agcgattcgg
     5521 actcagacag tgattcagat tcagactcag atagcgactc agattctgac agcgattcag
     5581 actcagacag cgactcagac tcagacagtg attcagattc agacagcgac tcagattcag
     5641 atagcgactc agactcagat agcgactcag actcagatag cgactcagac tcggatagcg
     5701 attcagattc agacagcgac tcagattcag atagcgattc ggactcagac aacgactcag
     5761 attcagatag cgattcagat tcagatgcag gtaaacatac tccggctaaa ccaatgagta
     5821 cggttaaaga tcagcataaa acagctaaag cattaccaga aacaggtagt gaaaataata
     5881 attcaaataa tggcacatta ttcggtggat tattcgcggc attaggatca ttattgttat
     5941 tcggtcgtcg taaaaaacaa aataaataat atgattaact taaccaggtc catgtggcct
     6001 ggttttttct gtttagaaat tcccagatat aaaacaaaaa atctttaagg aatagataca
     6061 atttaatttg ttacagaaat gtaattgtct atgaattaga aaagctaaga tattaatttc
     6121 aagggcaaat aatactattt tttatgatga ttaaataaag attacaaaac tcataaaaac
     6181 ttttatttaa acacaaatgt taaataacgt tttacgataa agaaaaataa ttaaagtatt
     6241 gtgctttatc taaaaatgta atactatatt gatatacatt tttgtattta aaacaattgt
     6301 attatttaaa aatttgatga attaggagta atctaatgct aaacagagaa aataaaacgg
     6361 caataacaag gaaaggcatg gtatccaatc gattaaataa attttcgatt agaaagtaca
     6421 cagtgggaac agcatcaatt ttagtaggta caacattaat ttttggtctg gggaaccaag
     6481 aagcaaaggc tgcagaaagt actaataaag aattgaacga agcgacaact tcagcaagtg
     6541 ataatcaatc gagtgataaa gttgatatgc agcaactaaa tcaagaagac aatactaaaa
     6601 atgataatca aaaagaaatg gtatcatctc aaggtaatga aacgacttca aatgggaata
     6661 aattaataga aaaagaaagt gtacaatcta ccactggaaa taaagttgaa gtttcaactg
     6721 ccaaatcaga tgagcaagct tcaccaaaat ctacgaatga agatttaaac actaaacaaa
     6781 ctataagtaa tcaagaagcg ttacaacctg atttgcaaga gaataaatca gtggtaaatg
     6841 ttcaaccaac taatgaggaa aacaaaaagg tagatgccaa aactgaatca actacattaa
     6901 atgttaaaag tgatgctatc aagagtaatg atgaaactct tgttgataac aatagtaatt
     6961 caaataatga aaataatgca gatatcattt tgccaaaaag tacagcacct aaacgtttga
     7021 atacaagaat gcgtatagca gcagtacagc catcatcaac agaggctaaa aatgttaatg
     7081 atttaatcac atcaaataca acattaactg tcgttgatgc agataaaaac aataaaatcg
     7141 taccagccca agattattta tcattaaaat cacaaattac agttgatgac aaagttaaat
     7201 caggtgatta tttcacaatt aaatactcag atacagtaca agtatatgga ttgaatccgg
     7261 aagatattaa aaatattggt gatattaaag atccaaataa tggtgaaaca attgcgactg
     7321 caaaacatga tactgcaaat aatttaatta catatacatt tacagattat gttgatcgat
     7381 ttaattctgt acaaatggga attaattatt caatttatat ggatgctgat acaattcctg
     7441 ttagtaaaaa cgatgttgag tttaatgtta cgataggtaa tactacaaca aaaacaactg
     7501 ctaacattca atatccagat tatgttgtaa atgagaaaaa ttcaattgga tcagcgttca
     7561 ctgaaacagt ttcacatgtt ggaaataaag aaaatccagg gtactataaa caaacgattt
     7621 atgtaaatcc atcggaaaat tctttaacaa atgccaaact aaaagttcaa gcttaccact
     7681 caagttatcc taataatatc gggcaaataa ataaagatgt aacagatata aaaatatatc
     7741 aagttcctaa aggttataca ttaaataaag gatacgatgt gaatactaaa gagcttacag
     7801 atgtaacaaa tcaatacttg cagaaaatta catatggcga caacaatagc gctgttattg
     7861 attttggaaa tgcagattct gcttatgttg taatggttaa tacaaaattc caatatacaa
     7921 atagcgaaag cccaacactt gttcaaatgg ctactttatc ttcaacaggt aataaatccg
     7981 tttctactgg caatgcttta ggatttacta ataaccaaag tggcggagct ggtcaagaag
     8041 tatataaaat tggtaactac gtatgggaag atactaataa aaacggtgtt caagaattag
     8101 gagaaaaagg cgttggcaat gtaactgtaa ctgtatttga taataataca aatacaaaag
     8161 taggagaagc agttactaaa gaagatgggt catacttgat tccaaactta cctaatggag
     8221 attaccgtgt agaattttca aacttaccaa aaggttatga agtaacccct tcaaaacaag
     8281 gtaataacga agaattagat tcaaacggct tatcttcagt tattacagtt aatggcaaag
     8341 ataacttatc tgcagactta ggtatttaca aacctaaata caacttaggt gactatgtct
     8401 gggaagatac aaataaaaat ggtatccaag accaagatga aaaaggtata tctggcgtaa
     8461 cggtaacatt aaaagatgaa aacggtaacg tgttaaaaac agttacaaca gacgctgatg
     8521 gcaaatataa atttactgat ttagataatg gtaattataa agttgaattt actacaccag
     8581 aaggctatac accgactaca gtaacatctg gtagcgacat tgaaaaagac tctaatggtt
     8641 taacaacaac aggtgttatt aatggtgctg ataacatgac attagatagt ggattctaca
     8701 aaacaccaaa atataattta ggtaattatg tatgggaaga tacaaataaa gatggtaagc
     8761 aggattcaac tgaaaaaggt atttcaggcg taacagttac attgaaaaat gaaaacggtg
     8821 aagttttaca aacaactaaa acagataaag atggtaaata tcaatttact ggattagaaa
     8881 atggaactta taaagttgaa ttcgaaacac catcaggtta cacaccaaca caagtaggtt
     8941 caggaactga tgaaggtata gattcaaatg gtacatcaac aacaggtgtc attaaagata
     9001 aagataacga tactattgac tctggtttct acaaaccgac ttacaactta ggtgactatg
     9061 tatgggaaga tacaaataaa aacggtgttc aagataaaga tgaaaagggc atttcaggtg
     9121 taacagttac gttaaaagat gaaaacgaca aagttttaaa aacagttaca acagatgaaa
     9181 atggtaaata tcaattcact gatttaaaca atggaactta taaagttgaa ttcgagacac
     9241 catcaggtta tacaccaact tcagtaactt ctggaaatga tactgaaaaa gattctaatg
     9301 gtttaacaac aacaggtgtc attaaagatg cagataacat gacattagac agtggtttct
     9361 ataaaacacc aaaatatagt ttaggtgatt atgtttggta cgacagtaat aaagacggca
     9421 aacaagattc aactgaaaaa ggtatcaaag atgttaaagt tactttatta aatgaaaaag
     9481 gcgaagtaat tggaacaact aaaacagatg aaaatggtaa atactgcttt gataatttag
     9541 atagcggtaa atacaaagtt atttttgaaa agcctgctgg cttaacacaa acaggtacaa
     9601 atacaactga agatgataaa gatgcagatg gtggcgaagt tgacgtaaca attacggatc
     9661 atgatgattt cacacttgat aatggatact acgaagaaga aacatcagat agcgactcag
     9721 attcggacag cgactcagat tcagacagag actcagactc agatagtgat tcagactcgg
     9781 atagcgattc agattcagac agcgattcag attcagatag cgattcagat tcagacagag
     9841 actcagatag tgattcagac tcagatagcg actcagattc agacagcgac tcagattcag
     9901 acagcgactc agactcagat agtgattcag actcagatag cgactcagat tcagacagcg
     9961 actcagactc agacagcgac tcagactcag atagtgactc agattcagat agcgactcag
    10021 attcggacag cgattcagac tcagatagcg actcagattc agatagcgat tcggactcag
    10081 atagcgactc agattcagat agtgattcag actcagatag cgactcagat tcagacagcg
    10141 actcagattc ggatagcgac tcagattcag acagcgactc agactcggat agtgattcag
    10201 actcagatag cgactcagac tcagatagcg attcagattc agatagcgac tcagactcag
    10261 acagcgattc agactcagac agcgactcag actcagatgc aggtaagcac acacctgtta
    10321 aaccaatgag tactactaaa gaccatcaca ataaagcaaa agcattacca gaaacaggta
    10381 atgaaaatag cggctcaaat aacgcaacgt tatttggcgg attattcgca gcattaggat
    10441 cattattgtt attcggtcgt cgtaaaaaac aaaataaata atacagctta taccaggtcc
    10501 gtgaggcctg gttttttatt tcaaaattta tgaaatgtat cttatgtttg agattaaata
    10561 gtgcaatttt ttaaaaaata taacataaat caacataaat ttaattcatc tttacattaa
    10621 aagtacaaac gaataagaaa agcaattaaa cacagtatca aaccatttaa aacaattgat
    10681 ataaaaaaca aacgaataat taaaaagtac tagaattaat ttattcaaac caaagtgtaa
    10741 aataacagtc ttagataaat aaatttattt aaagtattgt gctttatcta aaaatgtatt
    10801 acgatgggaa tacaaatttt tatatgtaaa aatattaatt ttttgcaaat attgatattt
    10861 taaaggagat ttatatgatt aacagggata ataaaaaggc aataacaaaa aagggtatga
    10921 tttcaaatcg cttaaacaaa ttttcgatta gaaagtatac tgtaggaact gcatcgattt
    10981 tagtaggtac gacattgatt tttggtctag ggaaccaaga agctaaagct gctgaaaaca
    11041 ctagtacaga aaatgcaaaa caagatgatg caacgactag tgataataaa gaagtagtgt
    11101 cggaaactga aaataattcg acaacagaaa ataattcaac aaatccaatt aagaaagaaa
    11161 caaatactga ttcacaacca gaagctaaaa aagaatcaac ttcatcaagt actcaaaaac
    11221 agcaaaataa cgttacagct acaactgaaa ctaagcctca aaacattgaa aaagaaaatg
    11281 ttaaaccttc aactgataaa actgcgacag aagatacatc tgttatttta gaagagaaga
    11341 aagcaccaaa taatacaaat aacgatgtaa ctacaaaacc atctacaagt gaaccatcta
    11401 caagtgaaat tcaaacaaaa ccaactacac ctcaagaatc tacaaatatt gaaaattcac
    11461 aaccgcaacc aacgccttca aaagtagaca atcaagttac agatgcaact aatccaaaag
    11521 aaccagtaaa tgtgtcaaaa gaagaactta aaaataatcc tgagaaatta aaagaattgg
    11581 ttagaaatga tagcaataca gatcattcaa ctaaaccagt tgctacagct ccaacaagtg
    11641 ttgcaccaaa acgtgtaaac gcaaaaatgc gctttgcagt tgcacaacca gcagcagttg
    11701 cttcaaacaa tgtaaatgat ttaattaaag tgacgaagca aacaatcaaa gttggcgatg
    11761 gtaaagataa tgtggcagca gcgcatgacg gtaaagatat tgaatatgat acagagttta
    11821 caattgacaa taaagtcaaa aaaggcgata caatgacgat taattatgat aagaatgtaa
    11881 ttccttcgga tttaacagat aaaaatgatc ctatcgatat tactgatcca tcaggagagg
    11941 tcattgctaa aggaacattt gataaagcaa ctaagcaaat cacatataca tttacagact
    12001 atgtagataa atatgaagat ataaaatcac gcttaactct atattcgtat attgataaaa
    12061 aaacagttcc aaatgagaca agtttgaatt taacatttgc tacagcaggt aaagaaacaa
    12121 gccaaaatgt cactgttgat tatcaagatc caatggtcca tggtgattca aacattcaat
    12181 ctatctttac aaaattagat gaagataagc aaactattga acaacaaatt tatgttaacc
    12241 cattgaaaaa atcagcaacc aacactaaag ttgatatagc tggtagtcaa gtagatgatt
    12301 atggaaatat taaactagga aatggtagca ccattattga ccaaaataca gaaataaagg
    12361 tttataaagt taactctgat caacaattgc ctcaaagtaa tagaatctat gattttagtc
    12421 aatacgaaga tgtaacaagt caatttgata ataaaaaatc atttagtaat aatgtagcaa
    12481 cattggattt tggtgatatt aattcagcct atattatcaa agttgttagt aaatatacac
    12541 ctacatcaga tggcgaacta gatattgccc aaggtactag tatgagaaca actgataaat
    12601 atggttatta taattatgca ggatattcaa acttcatcgt aacttctaat gacactggcg
    12661 gtggcgacgg tactgttaaa cctgaagaaa agttatacaa aattggtgac tatgtatggg
    12721 aagacgttga taaagacggt gttcaaggta cagattcaaa agaaaaacca atggcaaacg
    12781 ttttagttac attaacttac ccggacggta ctacaaaatc agtaagaaca gatgctaatg
    12841 gtcattatga attcggtggt ttgaaagacg gagaaactta tacagttaaa ttcgaaacgc
    12901 caactggata tcttccaaca aaagtaaatg gaacaactga tggtgaaaaa gactcaaatg
    12961 gtagttcggt tactgttaaa attaatggta aagatgatat gtctttagat actggttttt
    13021 acaaagaacc taaatacaac ttaggtgact atgtatggga agatactaat aaagatggta
    13081 tccaagatgc aaatgagcca ggaatcaaag atgttaaggt tacattaaaa gatagtactg
    13141 gaaaagttat tggtacaact actactgatg cctcgggtaa atataaattt acagatttag
    13201 ataatggtaa ctatacagta gaatttgaaa caccagcagg ttacacgcca acggttaaaa
    13261 atactacagc tgatgataaa gattctaatg gtttaacaac aacaggtgtc attaaagatg
    13321 cagataatat gacattagac agtggtttct ataaaacacc aaaatacagt ttaggtgatt
    13381 atgtttggta cgacagtaat aaagacggca aacaagattc aactgaaaaa ggtatcaaag
    13441 atgtgacagt tacattgcaa aacgaaaaag gcgaagtaat tggaacaact aaaacagatg
    13501 aaaatggtaa atatcgtttc gataatttag atagcggtaa atacaaagtt atttttgaaa
    13561 agcctgctgg cttaacacaa acagttacaa atacaactga agatgataaa gatgcagatg
    13621 gtggcgaagt tgacgtaaca attacggatc atgatgattt cacacttgat aacggatact
    13681 tcgaagaaga tacatcagac agcgattcag actcagatag tgactcagac agcgactcag
    13741 actcagacag cgactcagac tcagacagtg attcagattc agacagcgac tcagattcag
    13801 atagcgactc agattcggac agcgattcag actcagatag cgactcagat tcagatagcg
    13861 attcagactc agacagcgac tcagattcag atagcgattc ggactcagac agcgattcag
    13921 actcagatag cgactcagac tcagacagcg actcagattc agatagcgat tcggactcag
    13981 atagcgactc agattcagac agcgattcag actcagatag cgactcagac tcagacagtg
    14041 attcagattc agacagcgac tcagactcag atagcgactc agattcggac agcgactcag
    14101 actctgatag cgactcagac tcagacagtg attcagacag cgattcagac tcggatgcag
    14161 gaaaacatac acctgttaaa ccaatgagta ctactaaaga ccatcacaat aaagcaaaag
    14221 cattaccaga aacaggtagt gaaaataacg gctcaaataa cgcaacgtta tttggtggat
    14281 tatttgcagc attaggttca ttattgttat tcggtcgtcg caaaaaacaa aacaaataat
    14341 acaatatgac ccaggtcctt gtggcctggt ttttttataa ttacacatgc aatagatgta
    14401 tttttcatat aaaataaaca ataaagatac ggaataaaac ttatatgagg cgataatatg
    14461 aattacattt taggaacaat tttagaaagt aaaattacag gtgtagaaaa agcgcaaata
    14521 aatagattga agttgttcaa acaacacggc atatcttcaa aatgtgtata tgttaaatgg
    14581 aatccttatt catacacata tgcgaagcaa catcagattg aaaatgatgt atttacaatg
    14641 tatgactatt ttcaaaaagc aatcaattat aaaaagacaa agcaagttaa ctggatacag
    14701 tattgggaaa agtcatgtag gtacacattg aaatttgtgg aaaattcaaa tgatgtcaga
    14761 atatatgatg aagagcaatt tgtaatgtat gctcattttt tagataaaca gtatcatcaa
    14821 ttaaattatg tgaattattt tgatcataaa agaagaaaag taaaacgcga attgtatgat
    14881 ggaagaggct ttttaagttg ttctcgaatt ttaggtgaag gacaacggat tgtactcgaa
    14941 aattactata cacctaatgg ggaaatcgtc atccaaaaat attttgacga tataaaaggg
    15001 aaaaacacgc tcacaaaggt tatcttaaat gaagaccagc atcaacaatt ttttgataca
    15061 gaagatgaat tagttcaata ttttctccat caattatgta aaaataatga tcaaatcata
    15121 ttagatcgtc ctcatgaatt aggaaatgtt atagcgggat taaatcaaag tattccagtt
    15181 attgttgtac tccatagtac acatttatcc ggtgccggta atggtataaa aagtttttat
    15241 aaaacagtgt ttaataattt aacacgttat aaagcgattg ttgtatcaac agaaaagcaa
    15301 tgccaagata tttcacaata tattgaaaat aaaataccag ttatcaatat tccggttggc
    15361 tacgtggcaa atttaaagta tcaatttgac atcaatcaaa aggagaaaaa tcatatcata
    15421 tcaattgctc gcctcgttga aaataaacaa attaaacatc aaattgaagt aatcaagcaa
    15481 ttagtaacaa aacatcccaa tattcaattg aatatttatg gacatggaaa tggtttgtca
    15541 gaatatcgac aacttgtaga agattatcat ttatcggaac atgttaaatt tcatggtttt
    15601 aagacgcata ttaatgaaga gattgctaaa gcagaactga tgttatcgac aagtaaaatg
    15661 gaaggttttg gcttagcaat tttagagtcg ctttcagtag gtacaccagt gatcagttat
    15721 gatgtagatt atggtccatc agaactgatt caagatggat ttaatggcta tttagtacct
    15781 caaggtgaca tcaatcaaat ggttgaaaag gtcgaccaat tactaaataa tactcaaaaa
    15841 ttgcaacagt tttcaattaa tagcatagaa tctgcacaac agtacaatgc aactactatc
    15901 agtacaaagt ggcaaaatat tttaaactaa gtcaaaagaa aaaagcattt tcctagaatc
    15961 atctatttaa caaacctcgt tcatacgaac ttacatgttt agttaaatga cataggaaaa
    16021 tgctattttt gtaaaaataa aggtaaagta catttatata agacgaacaa attggtccca
    16081 ttgtttaatt aacgacgctt tactatattg ttgtgctttt gccaaactac cttttgacag
    16141 tcgttgctgt acttcaggat gatcaatcac atattttact ttatcaaata gggcatcttc
    16201 atcattttta gtaattaaat aaccattgaa atctgaagta atcagttcgt taggtccata
    16261 tttaatatca taactaataa ctggaacacc atgtgctaaa gattcaagta gcgctaaaga
    16321 gaaaccttcc atgttacttg ttattaaact caaataggca tcgctatatt cttggtctag
    16381 attgcttaaa aagccgcgta agtaaacatg attttccaat ccatattttt gtatcaattc
    16441 atttaatttt ttactttcag aaccaaaacc atacatatga agctctattt ttgggacata
    16501 cgatactaag cgtttaatta attcaatttg ttgatgtaat tgtttttcag gtgaataacg
    16561 agcaacggaa attaatttaa cactgcgctg atctaatgtt tggactggtg tatcaattgt
    16621 ttcactatag ccgacaggaa tattaacaac tggaatagta tggttaatac gtttttcaac
    16681 atctaatttt tgctgctcag tagaaacgat aattgcacga tatcgagata aattttcaaa
    16741 catcgcttta tatacatttt taaatggcga tgaatctaat gcatcaatat ttttaatgtg
    16801 tgtactgtga agcacagcta ctactgggat tgactcaggc gttaagttga aaataggtgc
    16861 tgtgtacaca ttacgatcac tgaaaaataa atccccatgt tgatatagtt gtttaatgaa
    16921 aaatgcgcct aattccgttt cattattaaa gaaatattgt ttgttagcat agtaaacaat
    16981 aattttttgt acttctggtt tgccatcctt gtaagaaaaa tacttttcta attttgtgtc
    17041 accttctgga ttatagaaaa attcacataa tgtttgttgt ttatcaacaa gaatcctact
    17101 acaacttaaa aagccacgca catcataaaa atcacgtttt acttttcgtc tttgactatc
    17161 aaaatgattt acataatcta atatacgata tttaggatct tgaaaatggg catacattaa
    17221 gaaacgctct tgatcatata ttctaaagtc atgactattt tcaacatgtt ttaaagtata
    17281 atgacattca tcagtccaat acgacaacca gtcaaatggt tcattgcgtt ctaaatatgt
    17341 tgcttcttgg aagaaatcat acatattaat atagtcagaa ctagtaatat aattttgggc
    17401 atttctatat aaatatctat tccatgacag aaatacacat tgcgctggtc ttcccatttc
    17461 tttaaataaa tttaaacgat taataattgc tttctctatc ccagttaaat taacacctaa
    17521 actattacct acaaaataat tcatttacaa caccacttat atctattttt tataattata
    17581 tcacataata tttaattact tcttttaact ggaagatgtg tttatttata aaacaacaaa
    17641 ttttgatatt tataatgata gtagttattc aatcaactac gaccaatata tcattgtaga
    17701 gcttaggata ttgatttatg actcaggcac atcaaatgag aagatttata aaagagatat
    17761 acaactctag aaggtataat aaaaacgcgc aactaatgtt acgcgtttga attaatcata
    17821 tgatattatt tgcgatactt taatttagcg aaagcatcat gttgatggat agactcttca
    17881 ttacgacatt cgatatcgaa accgtctaac caatcaaatt caactaagtc cgcggcaatt
    17941 aaacgaatta agtcttcgac aaaacgtgga ttttcatatg cacgctctgt cacacgtttt
    18001 tcatcaggac gttttaaaat agggtataga attgaacttg cattagcttc cattgcatct
    18061 aaaattttat ttttatagtc atcaactatg tcttgatctt tattaatata tgttttaaca
    18121 gtgacaacac cacgttggtt gtgcgctgaa tactcactta tttcttttga acaagggcat
    18181 agcgttgtga cagttgcttc aatagtaagt tctttacgtg taactttatc accgtcaatt
    18241 gctaatccat aagtgacatc ggcattacca actgctttaa tatttgtggt tggactatag
    18301 cgatcaaaga accatttccc agaaacatca acgcctgccg cattttgttt catattcgtt
    18361 tgtaaagtgc gtaacacctg ataaagtgta ttaaattcaa gttcaatacc attatcatag
    18421 tgcttttcaa cactttcgat tatacggctc atattaatac ctttttcgtc ttttgttaaa
    18481 cttgttgaaa aactaaatgt gccagctgtt tgatactggt caacaagtac agggtacact
    18541 aagtttttaa taccaacttc ttctatttca aataaaaaat ctttatgtgt actttgtaaa
    18601 tctgtcattt cgttcttagt agtaggtttc gtgccttcaa taggatctac ggaaccaaag
    18661 tgtttccaac gaccttctcg tgtcgataaa tcaaattcag tcattttttt cctccgttaa
    18721 gatttaaagt gatatgtcca atatggttcg actgttaaaa agctgtgttg tttaccatcg
    18781 atttcaggac ttgctaattg ttttaaaaat ggacctgttt gagaagcatg tgcttcaaat
    18841 gccttaattt taagttcttt aaaatctgta atatcatttt gaatatcagg ttctccaaga
    18901 gcttcggttg catcattact gaacgcaact aaagttaaac gagggcgttc ttctttaggc
    18961 atgcgttcaa ccgttcgaat tacagcgtct gctgttgctt cgtgatcagg atgtactgca
    19021 tatccaggat aaaatgaaat aatcaatgat ggatttgtat catcgattaa agatttaatc
    19081 ataccatcta tatgttcata gggttcaaat tcgacagttt tgtcacgtaa acccattttt
    19141 cttaaatcag taataccgat aactttacaa gcttcttcta gttcacgctc acgaatactt
    19201 ggtaatgatt cgcgtgttgc aaatggggga ttacctaaat ttctgcccat ttgtcctagg
    19261 gttaaacatg catatgttac aggtatgcct ttttggatat aatttgctaa tgtgcctgca
    19321 gatgagaagg tttcatcatc aggatgtgga aatattacta atacatgtct ttcgtcagtc
    19381 atgttgatgc ctcctctata aattaaatgg tcgctcacta atttgaagtg ctgcagcgag
    19441 ttgaccttcg taattaaaac ctgcaattaa aaattcatca tgctcattga cctcaaaatg
    19501 cgttagacct tgtacataaa cccaaccacc atttgatagt ttaagaccaa tgcgataagg
    19561 ttctttatta ccacctttta gttgtgcatg cgtatatgtt atttgtatgt ttcttaaaaa
    19621 agtaccagca ttaaaaacac gttgatcgaa atggttcgca taggccccat ttgtcgtttc
    19681 aacatgcaga tacacaggtt tatgttcaaa agaagcaagt aaatctataa cttcttgttc
    19741 tttaattggt tccaacacgt tcactcctta cactatcaat gtgtttatct ttctatttta
    19801 ctaaaaacta ttcgataatt gtatacgatt gctcaattat ttataaatta attttcatga
    19861 agggtaatta ctcaggatta cgtaatcata cagcattagt tttttacttt taaaaatcaa
    19921 aaatttgttg gaatttgaaa agtgttaaac attaaaaatg atgctatatt aatggtgtat
    19981 gaatgaattc ataagttttt aaaatgtatt aaatttgtgg aggcatgtaa acaatgaaag
    20041 tattaaactt aggatcgaaa aaacaagcat cattctatgt tgcatgtgag ttatataaag
    20101 agatggcatt taatcagcac tgtaaactag gtttagcaac tggtggtaca atgacagatt
    20161 tgtatgaaca acttgttaag ttattaaata aaaatcagtt aaacgtagac aatgtatcca
    20221 cgtttaattt agacgaatat gtaggtttaa ccgcatcaca tccgcaaagt tatcactatt
    20281 atatggatga catgcttttc aaacaatatc cttattttaa tagaaagaac attcatattc
    20341 caaatggaga tgccgatgat atgaatgcgg aagcgtcaaa atataatgac gttttagaac
    20401 aacaaggtca acgtgatatt caaattttag gtattggtga aaatggtcat attggattta
    20461 atgaacctgg tacgccgttt gatagcgtta ctcatatcgt tgatttgact gaaagtacta
    20521 ttaaggctaa tagtcgatat tttaaaaacg aagatgatgt tccaaagcaa gccatttcga
    20581 tgggacttgc taatattctt caagccaaac gtatcatttt actcgcattt ggtgaaaaga
    20641 aacgtgctgc tattacacat ttattaaatc aggaaatttc tgttgatgtt ccagccacat
    20701 tacttcacaa acacccgaat gttgagatat atttagacga cgaagcttgc ccgaaaaatg
    20761 ttgcgaaaat tcatgtcgat gaaatggatt gattgcaatg tttaattaag aaatgcctcg
    20821 ggaaaggttc caatagaaag ataaaaagca ttggaaggat gatttttagt ggaattacaa
    20881 ttagcaattg atttattaaa caaagaagac gcggctgagt tagcaaataa agtaaaagat
    20941 tatgtagata tcgtagaaat cggtacgcca atcatttaca acgaaggttt accagcagtt
    21001 aaacatatgg cagacaacat tagtaatgta aaagtattag cagacatgaa aattatagat
    21061 gcagctgatt atgaagttag ccaagcaatt aaatttggcg cggatgtaat tacaatacta
    21121 ggtgttgcag aagatgcatc aattaaagca gctattgaag aagctcataa aaataataaa
    21181 caattactag ttgatatgat tgctgttcaa gatttagaaa aacgtgcaaa agaactagat
    21241 gaaatgggtg ctgattatat tgcagtacac actggttatg atttacaagc agaagggcaa
    21301 tcaccattag aaagtttaag aaccgttaaa tctgttatta aaaattctaa agttgcagta
    21361 gcaggtggaa ttaaaccaga tacaattaaa gatattgtcg ctgaaagtcc tgatcttgtt
    21421 attgttggtg gcggaatcgc aaatgcagat gatccagtag aagctgcaaa acaatgtcgc
    21481 gctgcaatcg aaggtaagta atatggctaa atttagtgac tatcaattaa ttctagatga
    21541 attaaagatg actttgtcac atgttgaagc ggatgagttt tcaacttttg catccaaaat
    21601 actacatgct gaacatatat ttgtagctgg caaaggacgt tcaggattcg tggcgaatag
    21661 ttttgcaatg cgcttaaatc agctcggcaa acaggcacat gttgttggag aatcaacgac
    21721 acctgcgatt aagtcgaatg atgtatttgt aattatctct ggttcaggtt ccacggaaca
    21781 tttaagatta ttagcagaca aagcaaaatc agtaggtgct gacatcgtat taattactac
    21841 aaataaagat tctgcaatag gcaatctagc tgggacgaac atcgttttgc ctgcaggtac
    21901 aaaatatgat gaacaaggct cggcacaacc attaggaagt ttgtttgaac aagcatctca
    21961 attattttta gatagtgttg taatgggatt gatgactgaa atgaatgtta cggaacaaac
    22021 gatgcaacaa aatcatgcta atttagaata aaataaagat agtcgataat atgatgccta
    22081 ggcagaaata ttatcgatta tttttttatt taaataataa attatagtat aatatcaata
    22141 ataaacgaat aggggtgtta atattgaagt ttgacaatta tatttttgat tttgatggta
    22201 cgttggcaga cacgaaaaaa tgtggtgaag tagcaacaca aagtgcattt aaagcatgtg
    22261 gcttaacgga accatcatct aaagaaataa cgcattatat gggaatacct attgaagaat
    22321 catttttaaa attagcagac cgaccattag atgaagcagc attagcaaag ttaatcgata
    22381 catttagaca tacatatcaa tctattgaaa aggactatat ttatgaattt gcgggtataa
    22441 ctgaagccat tacaagtttg tataaccaag ggaaaaaact tttcgtggtg tctagtaaga
    22501 agagtgatgt attagaaaga aatttatcgg ctattggatt aaatcacttg attaccgaag
    22561 ctgttggatc cgatcaagta agtgcatata aaccaaatcc tgaaggcata cacacaattg
    22621 tgcaacgcta caatttaaat agccaacaaa cggtgtatat tggtgattca acgtttgatg
    22681 ttgagatggc acaacgtgct ggtatgcaat ctgcagctgt cacttggggt gcacatgatg
    22741 caaggtcatt acttcattca aatccggatt ttattattaa tgatccatca gaaattaata
    22801 ccgtattata aaacttgtta aaacagagaa taccatggtt aaaatgcata ttcataaata
    22861 ttagattata cttagaaata tttcgcttta gattaggaat ttaaaataaa tatttattaa
    22921 acattatgaa tttttaaaga gtaatgtctg actcgttgat aatttatttt tgtaaaaata
    22981 aattaaagta atgacaaagt tattgaagta aattgagtat aaacatttaa atacgatgtc
    23041 gaaaatggcg atagcatatc acttacatga agttatgtgc tatcgctatt tttagttata
    23101 attccaaaaa gttaatcgtt cgatgattta agaattatta ttgtttaatt caaatgtatg
    23161 agggtataaa atcattgaat ttaattcgat aaagcgaaat ttttgaaaaa acatactttt
    23221 gtatttatat aaaagtttaa attcttataa atttgacaaa actaattaac tccgtataat
    23281 tatgaaacat acaagaggga gtgtatgaat tcatggattt taataaagag aatattaaca
    23341 tggtggatgc aaagaaagct aaaaaaaccg ttgttgcaac cggtatcggt aatgcaatgg
    23401 aatggttcga ttttggtgtc tatgcatata caactgcgta cattggagcg aacttcttct
    23461 ctccagtaga gaatgcagac attcgacaaa tgttgacttt cgcagcatta gccattgcgt
    23521 ttttattaag accaattggt ggtgtcgtat ttggtattat tggtgacaaa tatggacgta
    23581 aagttgtatt aacatctaca attattttaa tggcattttc aacattaacc attggattat
    23641 tgccaagcta tgatcaaatt ggactttggg caccaatact attattgctt gcaagagtac
    23701 tacaagggtt ttcaacaggt ggagagtatg cgggggcaat gacatatgtt gccgaatcat
    23761 ctccagataa gcgtcgtaac tcattaggta gtggactaga aattgggaca ttatcaggtt
    23821 acatagctgc ttcaattatg attgctgtat taacattctt tttaacagat gaacaaatgg
    23881 catcatttgg ttggagaatc ccattcttac tcggtttatt cctaggatta ttcggcttat
    23941 atttacgtcg taagctggaa gaatcaccag ttttcgaaaa tgatgttgca acacaaccag
    24001 aaagagataa cattaacttt ttacaaatca tcagatttta ttacaaagat atatttgtat
    24061 gttttgtagc tgttgtattc ttcaatgtta caaactatat ggtaactgca tatttaccaa
    24121 cctatttaga acaagttatt aaattagatg caacgacaac aagtgtatta attacttgtg
    24181 tcatggcaat aatgattcca ttagcattaa tgtttggtaa gttagcggat aaaataggtg
    24241 aaaagaaagt atttctaatt ggtactggtg ggctaacatt attcagtatc atcgcattta
    24301 tgttattaca ttcacaatca tttgttgtaa tagtaatcgg tatatttata ttaggatttt
    24361 tcttatcaac ttacgaagcg acaatgccag ggtcgttacc aacgatgttt tacagtcata
    24421 taagatatcg aactttatca gtaacattta atatctctgt ttcgatattt ggtggtacga
    24481 cgccattagt tgcaacatgg ttagttacga aaactggaga tccattagca cctgcgtatt
    24541 atttaacagc aatcagtgtt attggctttt tagttattac attcttacat ttaagtacag
    24601 caggaaaatc tctaaaaggt tcgtatccaa atgtagataa cgagcaagat agagcttatt
    24661 atgcagaaca tccaaaagaa gcattatggt gggttaaaga acgtaagaat tagagatttt
    24721 aataaaaagt ataaatcaat cgtatataag cactttaaag ctagtaggtt ctgctaactt
    24781 taaagtgctt tttaaattga gaactgtaat tagccgtaat aaagtttttg tatatacata
    24841 aacccccact gcaatgatta tcgcaatggg ggaaagaggg gacttaaagc atatgtttag
    24901 ctttgaatac ttaaaattct cttgctattg aaatgttagg atgtaaatat gtcttagagt
    24961 attttgtcca acgcaattaa tattgagact ctaaccttca atattattat agagaacaca
    25021 aacttaaata gattgggtga cttatttgtg tcagttattg cgattgcgat aacttctttt
    25081 ctctatatac atatagtaac gtcttatcta ataaaaaaca tggtactaca gtatcaaatt
    25141 tatctagggc ttaagtttga tttttataat aggcaggttt acctgataaa aatacttatt
    25201 cattatataa tgttaacaat atgtatttta aagtttacat tgagtgaggg atattgatga
    25261 acgtaatttt agaacagttg aaaacacata ctcaaaataa acctaatgac atagcattac
    25321 atatcgatga tgaaacaatt acatatagtc aactaaatgc ccgcatcact agcgcagttg
    25381 aatctttgca gaaatattca cttaaccctg tcgttgctat taatatgaaa tcaccggtgc
    25441 aaagtattat ttgttattta gctttgcatc gtttacataa agtgcctatg atgatggaag
    25501 gtaaatggca aagtactata catcgtcaat tgattgaaaa atatggtatt aaagatgtaa
    25561 ttggagatac aggtctcatg cagaatatag actcaccgat gtttattgat tcaacgcaat
    25621 tacagcacta ccccaattta ttacatattg gttttacttc agggacaact ggactgccaa
    25681 aagcatatta tcgtgatgaa gattcatggt tggcttcttt tgaagttaat gaaatgttga
    25741 tgttaaaaaa tgaaaatgca atagcagccc ctggaccact atcgcactcg ttaacattat
    25801 atgcgttatt gtttgcttta agttccggtc gtacttttat aggacagacc acttttcatc
    25861 ctgaaaagtt acttaatcaa tgtcataaaa tatcatcata caaagttgct atgtttcttg
    25921 ttccaacgat gattaaatca ttattgttag tttacaacaa tgaacataca atccaatcat
    25981 tttttagcag tggagataag ctgcattctt ctatttttaa aaagataaaa aatcaagcaa
    26041 atgacataaa tttgattgaa ttttttggta catcggaaac cagttttatc agctataact
    26101 tgaatcagca agcaccagtt gaatcagtag gtgtgctatt tccaaatgtg gaattgaaaa
    26161 caacgaatca cgatcacaat ggtataggaa ctatttgtat aaaaagtaat atgatgttta
    26221 gtggctatgt aagtgaacaa tgtataaata atgatgaatg gtttgttact aatgataatg
    26281 gctatgtaaa agagcagtat ttatatttaa cgggacgtca acaggatatg ttaattattg
    26341 gtggtcaaaa tatatatcca gcacatgttg aacgcctttt aacgcaatct tcgagcattg
    26401 atgaagcaat tatcatcggt attccaaatg agcgttttgg tcaaataggc gtattgcttt
    26461 attctggtga tgtgacactt acacataaaa atgtaaaaca atttttaaaa aagaaagtga
    26521 aacgctatga aattccatcg atgattcatc atgtagaaaa gatgtattac actgcaagtg
    26581 gtaaaattgc tagagaaaaa atgatgtcga tgtatttgag aggtgaatta taatatgaat
    26641 caagcagtca tagttgcagc taaacgaact gcatttggga aatatggtgg cactttaaaa
    26701 catttagagc cagaacaatt gcttaaacct ttattccaac attttaaaga gaagtatcca
    26761 gaggtaatat ctaaaataga tgatgtagtt ttaggtaatg ttgttgggaa tggtggcaat
    26821 attgcaagaa aagcattgct tgaagcgggg cttaaagatt caatacctgg cgtcacaatc
    26881 gatcggcaat gtgggtctgg acttgaaagt gttcaatatg catgtcgcat gatccaagcc
    26941 ggagctggca aggtatatat tgcaggtggt gttgaaagta caagtcgagc accttggaaa
    27001 atcaaacgac cgcattctgt gtacgaaaca gcattacctg agttttatga gcgtgcatca
    27061 tttgcacctg aaatgagcga cccatcaatg attcaaggtg ctgaaaatgt ggccaagatg
    27121 tatgatgttt caagagaatt acaagatgaa tttgcttatc gaagtcatca attgacagcg
    27181 gaaaatgtaa aaaatggaaa tatttctcag gaaatattac ctataaccgt taaaggagaa
    27241 atattcaaca ctgatgaaag tctaaaatca catattccga aagataactt tggccgattt
    27301 aagcccgtga tcaaaggtgg gaccgttacc gctgcgaata gttgtatgaa aaatgatggt
    27361 gcagttttat tgcttattat ggaaaaagat atggcatacg aattaggttt cgagcatggt
    27421 ttattattta aagatggtgt tacggtaggt gttgattcta attttcctgg cattggtcca
    27481 gtaccagcca tttccaactt actaaaaaga aatcaattaa cgatagaaaa tattgaagtc
    27541 attgaaatta acgaagcgtt cagtgcacag gtagttgcct gccaacaagc tttaaatatt
    27601 tcaaatacgc aattaaatat atggggtggt gcattagcat caggtcatcc atacggtgca
    27661 agcggtgccc aattagtgac tcgattattt tatatgtttg acaaagagac tatgattgca
    27721 tctatgggga tagggggagg tctaggaaat gcagcattat ttactcgatt ctaaccagcg
    27781 attaaatgtg tcattttcta aggatagtgt ggctgcatat tatcagtgtt ttaaccaacc
    27841 ttatagaaaa gaagtaccac cattaatgtg tgcgtcatta tggccaaaat ttgatttatt
    27901 taaaaaatat gcaaatagcg aactgatttt aacaaaatca gcaattaatc aaactcaaaa
    27961 gatagaagta gacacaatat atgtagggca tttagaagat attgaatgcc gacagactcg
    28021 caatatcaca cgttatacaa tggctttaac attaactaaa aatgatcaac atgtcataac
    28081 ggttacacaa acttttatta aggcgatgaa gtagagatga agtttaatga gatatggata
    28141 aatgaatatt tggcgctcgt aaatgatgat aatccaatac ataatgagat tgtgccagga
    28201 caattagtga gtcaaatgat gctgatggct atgtcattag agacaaacca gtgtcaaatt
    28261 aactacgtta aacctatttt aataaatgaa aatatcgaat tcattgaaca acacgaacac
    28321 gaaattatag caattaatga cgatggagag attaaaataa aaatttcttt gagcacaaaa
    28381 aaataaccga tattagctgc atgaacgcat attaattagg agatgaaagg acagctaata
    28441 tcagttatgt attgttatta ttattgggaa cagagatgaa tataggttac gtttctttct
    28501 ttgcacgggg atgcattaat ctaaaataat aataacaact atatcaatgt ttaataaatt
    28561 ctggattatt ggaacgatta gtcaatttaa ctaactttca tatgatctat atcgtcttgt
    28621 aataaagaga gcaatttgaa tatttcagta tcactaaatg aatcgtcaca tttaattgaa
    28681 acatgctgaa acgttttggt tataatttca taaactggtg cgccttcatg gtgatactgt
    28741 cgataaataa tcataaccta tattacctcc tttgctactc tatggttata ttataaataa
    28801 catttttatg tgtgacatca accttaagta tcaacttttt atcagacata gaacgtatga
    28861 tttactaaga ctatttatgt ataaaagttc taaataaata tatatttata gagtcgcctg
    28921 gcagtcattt gggaaatata acatatatga ttagagaggc atctatcgca aaagaatgat
    28981 aatgatagag gtattgagca tatagatgag tttaagttca tcttgaaaat aaagggttat
    29041 ttagtcatag atgtagatgt ataggaaata tttgtatgta ttgttcgata tgtatgaaat
    29101 tttcaataaa agctaataac gcttatatgt aactttcaaa tttaaattat atacagagca
    29161 tgatgattat aaaaaaataa ccacatcaca taaattgagt tcatacccaa tttaagtggt
    29221 gtggctaata atgttgattt atagatgaac cgcctaatcg ttaaacctct gttacttcaa
    29281 catcgatatg ttcaatacgg ttgtatgcac cgtgatccac aggaccaaca aaatcattca
    29341 ttttccaacc gtttttaata gcagaagcga cgaaagcttt cgcgctaatc acagcttctt
    29401 tcggtgactt accgttagct aaatatgcag ttgttgccgc agcaaatgta caaccagcac
    29461 catggttata actttgttgg aacatgtctg ttgttagttg ataaaatgtt tgaccatcat
    29521 agtataagtc atacgattta tcttgatcta aagctttgcc acctttaatg atgacatgct
    29581 gtgcgccttt atcaaagata attgttgcag cctttttcat atcttcaatt gaatttaatt
    29641 tacctaatcc tgataattga cccgcttcaa ataagtttgg tgtcactacc gttgctttag
    29701 gtagtaaata tttaatcatc gcctcagtat ttccaggatt aagcacttca tcttcgcctt
    29761 tacaaaccat gacaggatct actacaaaat attgtgcatt agatgcctca tatacttctc
    29821 cagcacgttt gattatctcc tcagtaccta acatacctgt tttaatagca tcaggtccga
    29881 ttgataaagc cgtttcaagt tgtttttcaa atacatccat tggtaatggt gtaacatcgt
    29941 gtgaccatgt atctttatcc atagtaacga tggcagttaa agcgaccatg ccatacgtat
    30001 ctaattcttg gaacgttttc aaatctgctt gcatacctgc gccagcactt gtgtcagaac
    30061 cggcaattgt taaaactttc tttaaagcca ttgagcttca ctcctacata ataatattgt
    30121 attcatcata tcatttttaa cctaattgaa aaatattaag cattcaatat ttgatgattg
    30181 ttgaaatgaa tcattcatac tattgtaact tttgaaaatg tcattcactt tagataagtg
    30241 tgatatgtta aaatatgtcc tgaggtgaga ttgaatggaa tggtcgcaaa tttttcatga
    30301 cataacaacg aaacatgact ttaaagctat gcatgatttt ttagaaaaag aatattcgac
    30361 tgcaatcgta taccctgata gggaaaatat atatcaagcg tttgatttaa caccgtttga
    30421 aaatatcaaa gttgttatat taggacaaga cccgtatcat ggtccaaacc aagcacatgg
    30481 attagcattt tcagtgcaac ctaacgcaaa attccctcca tctttacgta atatgtataa
    30541 agaattagca gatgatattg gatgcgttag acaaacaccg catttacaag attgggcaag
    30601 agaaggcgtc ttgttattga atacagtttt aaccgtaaga cagggtgaag caaattctca
    30661 tcgtgatatt ggttgggaaa catttactga tgaaattatt aaagcagtgt ctgattataa
    30721 agaacatgtt gtctttattt tgtgggggaa acctgcacag caaaaaataa agcttatcga
    30781 tacatctaaa cattgtatta taaaatcagt gcatcctagt ccactgtctg catatagagg
    30841 attctttgga tcaaaaccgt attccaaagc gaatgcctat ttagagtcag taggaaaatc
    30901 accaattaat tggtgtgaaa gtgaggcgta gatgttgaat agagaaactt taatagcacg
    30961 aattgagcaa gaattagtac aagcagagca ggcacagcat gaccatgact ttgaaaaaca
    31021 tatgtatgcc atacatatat taacatcttt atatgcttca acatcaaata caccacatat
    31081 tggtgaacaa caaatgaatc gtcgtattgc taaccataat caaatgccac aatcacaaat
    31141 aacgcagcca actcatcaag tgacagttgc tgaaattgaa gcgatgggtg gtaaagtaaa
    31201 tacgcattca gcacatcatc ataataagtc atattcacaa ccttcaaacc aacaacaaag
    31261 attagcgaca gatgatgaca ttggcaatgg tgaatccata tttgattttt aaaaagcaac
    31321 aatgaaacat aattacttaa tagcttgtta agtatgtagg ttaataatca agacgcatat
    31381 acttttattc gagtgttcgg atttaaacat ttattaatac tgaattatat aaggagaggt
    31441 agcaatgaaa ttatttatta ttttaggtgc attaaacgcg atgatggctg tcggtacagg
    31501 tgcatttggt gcgcatggtt tacaaggaaa aataagtgat cactatttat cagtatggga
    31561 aaaagcaacg acgtatcaaa tgtaccatgg cttagcatta ttaattatag gtgtaattag
    31621 tggtacaact tcaatcaatg ttaactgggc tggctggtta atatttgctg gtattatttt
    31681 ctttagtgga tcattatata ttttagtatt aactcaaatt aaagttttag gtgcgattac
    31741 gccaattggt ggcgtattgt tcatcattgg atggataatg ttaatcattg cgacattcaa
    31801 atttgctggt taaattttaa aactttagat tacctatgta actaaacatt aaatttttaa
    31861 taaaaataat caagaaaaag agttacaaac tcatcttttg ggtatagaat accttcgagg
    31921 tgagttttta tttatggaaa aaaagaataa gcaaatagat agaggcgatt taaaacaaaa
    31981 cctatctgaa aagtttgtat gggcgattgc atatggttca tgtatcggat ggggcgcatt
    32041 catcttacca ggagactgga ttaagcagtc aggtccgatt gcagcatcaa ttggtatagt
    32101 tattggtgca ttattaatga tattaattgc ggttagttat ggcgcattag tagagagatt
    32161 tccagtatca gggggcgcgt ttgcctttag tttcttaagt ttcggcagat atgtgagttt
    32221 cttctcatca tggtttttaa cttttggtta tgtctgtgtc gttgctttaa atgcgaccgc
    32281 attcagttta ctagttaaat tcttattgcc agatgtctta aataatggga aactatacac
    32341 cattgcgggc tgggacgttt atattacgga aatcattatt gcgaccgtat tactatttgt
    32401 attcatgcta gtaacgattc gtggcgcaag tgtatctgga tcattacaat attatttctg
    32461 tgtggcgatg gtaatcgtcg tattattgat gttctttggt tcattctttg gtaataattt
    32521 tgcacttgaa aatttacaac cgttagctga acctagcaaa ggatggttag tgtctattgt
    32581 ggttattgta tccgtggcac catgggcata tgttggattt gataatattc cacaaacagc
    32641 agaagagttt aactttgcac caaacaagac atttaagctt atcgtgtaca gtttattagc
    32701 agcatcatta acttatgttg tcatgatttt atacactggt tggttatcaa caagtcatca
    32761 aagtttaaat gggcagttgt ggttaacagg tgctgttaca caaacagcat ttggttatat
    32821 tggattaggt gtattagcaa ttgcaattat gatgggtata tttactggtt taaatggatt
    32881 cttgatgagt tcaagtcgct tgttattttc tatgggacgt tcaggtatta tgccaacaat
    32941 gtttagtaaa ttacatagta aatacaaaac accatatgtc gcaatcatat tcctagtagg
    33001 agtgtcgtta attgcacctt ggctaggaag aactgcattg acttggattg tagatatgtc
    33061 atctactggt gtatccattg cctactttat tacatgtttg tctgcagcga aattattcag
    33121 ttataacaaa caaagtaata cgtatgcacc ggtttacaaa acgtttgcta ttatcggctc
    33181 atttgtatca ttcattttct tagcgttgtt attagtgcca ggttctcctg cagcactgac
    33241 tgcaccgtct tatattgcat tacttggatg gttaatcatc ggtttaatat tctttgtgat
    33301 tcgatatcct aaattgaaaa atatggataa tgatgaatta agtcgcttga ttttaaatag
    33361 aagtgaaaat gaagttgatg atatgattga agaacctgaa aaagaaaaaa ctaaataata
    33421 aaagaatcgc acaataaacc ttcttcattc ggaggcgtat cgtgcgattt tttgtattat
    33481 aaattgacat ttaagacgag gcagctgaac cttatatata attgctaaga gttagggctg
    33541 agccatttct aacaaatatt tataatcgtt taaaagattt cacgaaccca gaaacaatta
    33601 atttggaaat ttggtcggcg aataataaac ctaatgcgat ggcgcctgca ataagtgtaa
    33661 cctctagcat ggtattgatt gctgtactga aatttaataa gactaaattt tttgtagcat
    33721 cgtatgctaa gccaccaggt actaatggaa tgatacccgt taccataaaa atgatggcag
    33781 gttctttttg tttacgagcc atataatgac ttaacaagcc taatgctaaa ctaccaaaga
    33841 aactagagta tatagtgtgc acattaaagc cgttgaagaa taaggtgtaa accatccatc
    33901 cacacgtacc aacgaaacca catgatagat ataattttct aggtgcatca aaaatgacgc
    33961 agaagaacat tgaagctaaa aagctaaaga taaagtttaa gatccaaaac atagtctgat
    34021 actcctatac taaaattaat acgctaccaa cgccagcacc gatgccaaac gcagtaacca
    34081 atgcttctaa tgatttcgtt gtgaacatca acatgtgtcc accaaataaa tcttgtattg
    34141 cgtttgttat taatacacca ggaacaatag gcatgactgc cgcaatgata atagttgcca
    34201 agtcacctgt tggaataagt gtatgtccaa taacggcgat aatcccaata actaatgaac
    34261 caatgaattc tgggataaac tgtgcgtgta acttacgatc taaaatctca gtgactaggt
    34321 atcctagact acctgctaat atcgcagtta aaacatcaat caatctacca ccttgtaaat
    34381 ataagaaact cattgcaatc attgctgcag caaaaccttt aaagggaaga ctgctgtcac
    34441 gcttagcaac atatattttt tcaagttgcg tttttgcttc ggctaaagaa atttcattgt
    34501 ttgtaatttg acgcgaaatt ttattagctt gagaaatttt tattaagttt gtatctcgag
    34561 aggtaattct aaatattcta ggaaacgatt ccgaatgtaa cgtaaactgg atgacagtgt
    34621 ttgtaacaaa gctgttactt tcactgtaac caagtttttt tgcaatacgt gtcatggtat
    34681 cttctacacg cgtaccttct gcaccagatt ctaatagtat gcgagcagca agcatgacaa
    34741 cgtctttgat aagtacctct tgtttgtatt cttctgaatt tatgtccatt tcatcaccat
    34801 tgtttataag aatttaatac tcattatagt ttatacacta taaaataacc acatgagctt
    34861 tttgataagt tgttattgat tttaaaaaac taatatttga tactattgta ataataatca
    34921 tcggaatttt tagtagttaa aattctcttg aagtataaaa aggaaaaatt attgaaaaaa
    34981 attcgacaaa aatatgtttc aaatatttca aataggttaa aataatatta gttaaccatt
    35041 acaaaaattg tatagagtag cgactgtata atttctattg aggttaacgt ttatatgtag
    35101 tgatagtagt taaagttctc ccaaggaaga ctactcgggt acactttgct atgagcaaag
    35161 tgtactttgt tattgataat acattagcac atatatataa gtttaaacat acagatttca
    35221 actatttacc aaatcacgtc ttatgtatac gggaatatac tgaagataaa cgaaaaattc
    35281 caagcttaaa ccgataagct tggaattttt ttattaattt ataaacgtac caatgtatta
    35341 agaaatcgca aagaattgat cgaattcgtt tgtgttaata atatgtccta caaagaaact
    35401 accgaattca ccgtatcgtg ctgttgtttc atcaaagcgc atttcgtata caattttttt
    35461 gaattgtaat acgtcatctg agaacaatgt tacgccccat tcgaaatcat caaaccctac
    35521 agaaccagta ataaattgtt tgattttgcc agcatatttt ctaccaatca taccatggtc
    35581 atacattaat ttttggcgtt cttccatagt taacatgtac cagttataag tttcattacg
    35641 acgtttgttc attggataga aacaaatata atcagaatgt ggtaattctg ggtataatct
    35701 tgctttgata tgagggttct cataaggatc ttcatcagat ttaccagcta aataattgct
    35761 caattcaatg actgatacat atgaatatgt agggattagg aagtcagcaa tgcgcaattt
    35821 gttaaattca ttttcaatat gatttaaaga cttcatttca ggacgtaaga accataataa
    35881 caaatctgct ttttgaccag ttatattata aatagcttga tcaccagatt ttgatgatct
    35941 tacagttgct gtattttcta aaaatgattg aaattcagtg acaagtgcat cgcgttcgtc
    36001 ctttggaact atacgtaatg atgcccaatc aactgcataa aataaatgta gactatacca
    36061 accatctaat gtttcggctg cttgactcat gtttatcgct ccttttcaaa aatcatcagt
    36121 tctttataaa ttttatcata aagcaaaggg gtaacgataa aattatgatt acaaattggt
    36181 gacgtggcat tatgaaataa aatggcgtat aattataccg tgaatgatta ataagattta
    36241 tattacagga ggacattatg gctgatttat taaatgtatt aaaagacaaa ctttctggta
    36301 aaaacgttaa aatcgtatta cctgaaggag aggacgaacg tgttctaaca gctgcaacac
    36361 aattacaagc aacagattat gttacaccaa tcgtgttagg tgatgagact aaggttcaat
    36421 ctttagcgca aaaacttgat cttgatattt ctaatattga attaattaat cctgcgacaa
    36481 gtgaattgaa agctgaatta gttcaatcat ttgttgaacg acgtaaaggt aaagcgactg
    36541 aagaacaagc acaagaatta ttaaacaatg tgaactactt cggtacaatg cttgtttatg
    36601 ctggtaaagc agatggttta gttagtggtg cagcacattc aacaggcgac actgtgcgtc
    36661 cagctttaca aatcatcaaa acgaaaccag gtgtatcaag aacatcaggt atcttcttta
    36721 tgattaaagg tgatgaacaa tacatctttg gtgattgtgc aatcaatcca gaacttgatt
    36781 cacaaggact tgcagaaatt gcagtagaaa gtgcaaaatc agcattaagc tttggcatgg
    36841 atccaaaagt tgcaatgtta agcttttcaa caaaagggtc tgctaaatca gacgacgtga
    36901 caaaagttca agaagctgtc aaattagcac aacaaaaagc tgaagaagaa aaattagaag
    36961 caatcattga tggcgaattc caatttgatg ctgcgattgt accaggtgtt gctgagaaaa
    37021 aagcgccagg tgctaaatta caaggtgatg caaatgtctt tgtattccca agtttagaag
    37081 ctggtaatat tggttacaaa attgcacaac gtttaggtgg atatgatgca gttggtccag
    37141 tattacaagg tttaaattct ccagtaaatg acttatcacg tggctgctca attgaagatg
    37201 tatacaatct ttcaattatt acagcagcgc aagccttaca ataacgatgg atttagcgag
    37261 taaatatttt aatggcgtca actggcgata tatcgatcat tcttctggat tagaacctat
    37321 gcaatctttc gcattcgatg atacattttg cgaaagtgtg ggcaaagata tatcagataa
    37381 tgttgtgcgt acttggattc atcaacatac tgttattctt ggtattcatg attcaagatt
    37441 gccgttttta aaagatggca ttgattattt aacgaatgag attggttata atgccattgt
    37501 tagaaattct ggtggcttag gtgtcgttct agatcaaggt gtattaaata tatcgctgat
    37561 gttcaaagga caaacagaaa caacgattga tgaagcgttt actgtgatgt acctcttaat
    37621 tagcaaaatg ttcgaaaatg agaatgttga tattgatacg atggaaattg aacattctta
    37681 ttgcccagga aaatttgact taagtatcga tggtaagaaa tttgcaggca tatcgcaacg
    37741 aagagttaga ggcggtattg ctgtacaaat ttatctttgt gttgaaggct ctggttcaga
    37801 acgtgcattg atgatgcaaa cattttatga acatgcttta aaaggtgaag tgactaaatt
    37861 taaatatcct gaaattgaac catcttgtat ggcctcatta gagacattgc ttaacaaaac
    37921 gattactgtt caagatgtaa tgtttttact attatatgca atcaaagatc ttggcggtgt
    37981 attaaatatg acgccaatta ctcaagaaga atggcaaaga tacgatacgt attttgataa
    38041 aatgattgaa agaaacaaga aaatgataga tcaaatgcaa tagttaaata tttaacagcc
    38101 cctttcaagt tatcgtttgg ggctgttatt tttgaaagta tccataaaaa gcataaatat
    38161 gttattgacc taatatttaa attgaacatc attaaattaa aagtaatgat atgatggaaa
    38221 taataaattt aataagaaaa tttcatgttt tacgtattaa atagattgag aatagggtat
    38281 atataatgcg tatgactatg aaagaaacga ccttctattg aaatgttcta atactttagt
    38341 agtaaatcta tatattc
//
LOCUS       DAKTIP010000005        36991 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAKTIP010000005 DAKTIP010000000
VERSION     DAKTIP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 36991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36991
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.005"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1056
                     /gene="norB"
                     /locus_tag="O0305_000198"
     CDS             <1..1056
                     /gene="norB"
                     /locus_tag="O0305_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCY0273385.1"
                     /translation="GLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSF
                     FGGAVATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLL
                     SLNILITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGA
                     TASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLG
                     CKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIAN
                     APLEKVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGIL
                     SFVIILLLVPKQNDTQL"
     gene            1454..32719
                     /gene="ebh"
                     /locus_tag="O0305_000199"
     CDS             1454..32719
                     /gene="ebh"
                     /locus_tag="O0305_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
                     /protein_id="HCY0273386.1"
                     /translation="MNYRDKIQKFSIRKYTVGTFSTVIATLVFLGFNTSQAHAAETNQ
                     PASVVKQKQQSNNEQTENRESQVQNSQNSQNGQSLSATHENEQPNISQANLVDQKVAQ
                     SSTTNDEQPASQNVNTKKDSATAATTQPDKEQSKHKQNESQSANKNGNDNRAAHVENH
                     EANVVTASDSSDNGNVQHDRNELQAFFDANYHDYRFIDRENADSGTFNYVKGIFDKIN
                     TLLGSNDPINNKDLQLAYKELEQAVALIRTMPQRQQTSRRSNRIQTRSVESRAAEPRS
                     VSDYQNANSSYYVENANDGSGYPVGTYINASSKGAPYNLPTTPWNTLKASDSKEIALM
                     TAKQTGDGYQWVIKFNKGHAPHQNMIFWFALPADQVPVGRTDFVTVNSDGTNVQWSHG
                     AGAGANKPLQQMWEYGVNDPHRSHDFKIRNRSGQVIYDWPTVHIYSLEDLSRASDYFS
                     EAGATPATKAFGRQNFEYINGQKPAESPGVPKVYTFIGQGDASYTISFKTQGPTVNKL
                     YYAAGGRALEYNQLFMYSQLYVESTQDHQQRLNGLRQVVNRTYRIGTTKRVEVSQGNV
                     QTKKVLESTNLNIDDFVDDPLSYVKTPSNKVLGFYSNNANTNAFRPGGAQQLNEYQLS
                     QLFTDQKLQEAARTRNPIRLMIGFDYPDAYGNSETLVPVNLTVLPEIQHNIKFFKNDD
                     TQNIAEKPFSKQAGHPVFYVYAGNQGNASVNLGGSVTSIQPLRINLTSNENFTDKDWQ
                     ITGIPRTLHIENSTNRPNNARERNIELVGNLLPGDYFGTIRFGRKEQLFEIRVKPHTP
                     TITTTAEQLRGTALQKVPVNISGIPLDPSALVYLVAPTNQTTNGGSEADQIPSGYTIL
                     ATGTPDGVHNTITIRPQDYVVFIPPVGKQIRAVVYYNKVVASNMSNAVTILPDDIPPT
                     INNPVGINAKYYRGDEVNFTMGVSDRHSGIKNTTITTLPNGWTSNLTKADKNNGSLSI
                     TGRVSMNQAFNSDITFKVSATDNVNNTTNDSQSKHVSIHVGKISEDAHPIVLGNTEKV
                     VVVNPTAVSNDEKQSIITAFMNKNQNIRGYLASTDPVTVDNNGNVTLHYRDGSSTTLD
                     ATNVMTYEPVVKPEYQTVNAAKTATVTIAKGQSFSIGDIKQYFTLSNGQPIPSGTFTN
                     ITSDRTIPTAQEVSQMNAGTQLYHITATNAYHKDSEDFYISLKIIDVKQPEGDQRVYR
                     TSTYDLTTDEISKVKQAFINANRDVITLAEGDISVTNTPNGANVSTITVNINKGRLTK
                     SFASNLANMNFLRWVNFPQDYTVTWTNAKIANRPTDGGLSWSDDHKSLIYRYDATLGT
                     QITTNDILTMLKATTTVPGLRNNITGNEKSQAEAGGRPNFRTTGYSQSNATTDGQRQF
                     TLNGQVIQVLDIINPSNGYGGQPVTNSNTRANHSNSTVVNVNEPAANGAGAFTIDHVV
                     KSNSTHNASDAVYKAQLYLTPYGPKQYVEHLNQNTGNTTDAINIYFVPSDLVNPTISV
                     GNYTNHQVFSGETFTNTITANDNFGVQSVTVPNTSQITGTVDNNHQHVSATAPNVTSA
                     TNKTINLLATDTSGNTATTSFNVTVKPLRDKYRVGTSSTAANPVRIANISNNATVSQA
                     DQTTIINSLTFTETVPNRSYARASANEITSKTVSNVSRTGNNANVTVTVTYQDGTTST
                     VTVPVKHVIPEIVAHSHYTVQGQDFPAGNGSSASDYFKLSNGSDIADATITWVSGQAP
                     NKDNTRIGEDITVTAHILIDGETTPITKTATYKVVRTVPKHVFETARGVLYPGVSDMY
                     DAKQYVKPVNNSWSTNAQHMNFQFVGTYGPNKDVVGISTRLIRVTYDNRQTEDLTILS
                     KVKPDPPRIDANSVTYKAGLTNQEIKVNNVLNNSSVKLFKADNTPLNVTNITHGSGFS
                     SVVTVSDALPNGGIKAKSSISMNNVTYTTQDEHGQVVTVTRNESVDSNDSATVTVTPQ
                     LQATTEGAVFIKGGDGFDFGHVERFIQNPPHGATVAWHDSPDTWKNTVGNTHKTAVVT
                     LPNGQGTRNVEVPVKVYPVANAKAPSRDVKGQNLTNGTDAMNYITFDPNTNTNGITAA
                     WANRQQPNNQQAGVQHLNVDVTYPGISAAKRVPVTVNVYQFEFPQTTYTTTVGGTLAS
                     GTQASGYAHMQNATGLPTDGFTYKWNRDTTGTNDANWSAMNKPNVAKVVNAKYDVIYN
                     GHTFATSLPAKFVVKDVQPAKPTVTETAAGAITIAPGANQTVNTHAGNVTTYADKLVI
                     KRNGNVVTTFTRRNNTSPWVKEASAATVAGIAGTNNGITVAAGTFNPADTIQVVATQG
                     SGETVSDEQRSDDFTVVAPQPNQATTKIWQNGHIDITPNNPSGHLINPTQAMDIAYTE
                     KVGNGAEHSKTINVVRGQNNQWTIANKPDYVTLDAQTGKVTFNANTIKPNSSITITPK
                     AGTGHSVSSNPSTLTAPAAHTVNTTEIVKDYGSNVTAAEINNAVQVANKRTATIKNGT
                     AMPTNLAGGSTTTIPVTVTYNDGSTEEVQESIFTKADKRELITAKNHLDDPVSTEGKK
                     PGTITQYNNAMHNAQQQINTAKTEAQQVINNERATPQQVSDALTKVRAAQTKIDQAKA
                     LLQNKEDNSQLVTSKNNLQSSVNQVPSTAGMTQQSIDNYNAKKREAETEITAAQRVID
                     NGDATAQQISDEKHRVDNALTALNQAKHDLTADTHALEQAVQQLNRTGTTTGKKPASI
                     TAYNNSIRALQSDLTSAKNSANAIIQKPIRTVQEVQSALTNVNRVNERLTQAINQLVP
                     LADNSALKTAKTKLDEEINKSVTTDGMTQSSIQAYENAKRAGQTESTNAQNVINNGDA
                     TDQQIAAEKTKVEEKYNSLKQAIAGLTPDLAPLQTAKTQLQNDIDQPTSTTGMTSASI
                     AAFNEKLSAARTKIQEIDRVLASHPDVATIRQNVTAANAAKSALDQARNGLTVDKAPL
                     ENAKNQLQHSIDTQTSTTGMTQDSINAYNAKLTAARNKIQQINQVLAGSPTVEQINTN
                     TSTANQAKSDLDHARQALTPDKAPLQTAKTQLEQSINQPTDTTGMTTASLNAYNQKLQ
                     AARQKLTEINQVLNGNPTVQNINDKVTEANQAKDQLNTARQGLTLDRQPALTTLHGAS
                     NLNQAQQNNFTQQINAAQNHAALETIKSNITALNTAMTKLKDSVADNNTIKSDQNYTD
                     ATPANKQAYDNAVNAAKGVIGETTNPTMDVNTVNQKAASVKSTKDALDGQQNLQRAKT
                     EATNAITHASDLNQAQKNALTQQVNSAQNVQAVNDIKQTTQSLNTAMTGLKRGVANHN
                     QVVQSDNYVNADTNKKNDYNNAYNHANDIINGNAQHPVITPSDVNNALSNVTSKEHAL
                     NGEAKLNAAKQEANTALGHLNNLNNAQRQNLQSQINGAHQIDAVNTIKQNATNLNSAM
                     GNLRQAVADKDQVKRTEDYADADTAKQNAYNSAVSSAETIINQTTNPTMSVDDVNRAT
                     SAVTSNKNALNGYEKLAQSKTDAARAIDALPHLNNAQKADVKSKINAASNIAGVNTVK
                     QQGTDLNTAMGNLQGAINDEQTTLNSQNYQDATPSKKTAYTNAVQAAKDILNKSNGQN
                     KTKDQVTEAMNQVNSAKNNLDGTRLLDQAKQTAKQQLNNMTHLTTAQKTNLTNQINSG
                     TTVAGVQTVQSNANTLDQAMNTLRQSIANKDATKASEDYVDANNDKQTAYNNAVAAAE
                     TIINANSNPEMNPSTITQKAEQVNSSKTALNGDENLAAAKQNAKTYLNTLTSITDAQK
                     NNLISQITSATRVSGVDTVKQNAQHLDQAMASLQNGINNESQVKSSEKYRDADTNKQQ
                     EYDNAITAAKAILNKSTGPNTAQNAVEAALQRVNNAKDALNGDAKLIAAQNAAKQHLG
                     TLTHITTAQRNDLTNQISQATNLAGVESVKQNANSLDGAMGNLQTAINDKSGTLASQN
                     FLDADEQKRNAYNQAVSAAETILNKQTGPNTAKTAVEQALNNVNNAKHALNGTQNLNN
                     AKQAAITAINGASDLNQKQKDALKAQANGAQRVSNAQDVQHNATELNTAMGTLKHAIA
                     DKTNTLASSKYVNADSTKQNAYTTKVTNAEHIISGTPTVVTTPSEVTAAANQVNSAKQ
                     ELNGDERLREAKQNANTAIDALTQLNTPQKAKLKEQVGQANRLEDVQTVQTNGQALNN
                     AMKGLRDSIANETTVKTSQNYTDASPNNQSTYNSAVSNAKGIINQTNNPTMDTSAITQ
                     ATTQVNNAKNGLNGAENLRNAQNTAKQNLNTLSHLTNNQKSAISSQIDRAGHVSEVTA
                     TKNAATELNTQMGNLEQAIHDQNTVKQSVKFTDADKAKRDAYTNAVSRAEAILNKTQG
                     ANTSKQDVEAAIQNVSSAKNALNGDQNVTNAKNAAKNALNNLTSINNAQKRDLTTKID
                     QATTVAGVEAVSNTSTQLNTAMANLQNGINDKTNTLASENYHDADSDKKTAYTQAVTN
                     AENILNKNSGSNLDKTAVENALSQVANAKGALNGNHNLEQAKSNANTTINGLQHLTTA
                     QKDKLKQQVQQAQNVAGVDTVKSSANTLNGAMGTLRNSIQDNTATKNGQNYLDATERN
                     KTNYNNAVDSANGVINATSNPNMDANAINQIATQVTSTKNALDGTHNLTQAKQTATNA
                     IDGATNLNKAQKDALKAQVTSAQRVANVTSIQQTANELNTAMGQLQHGIDDENATKQT
                     QKYRDAEQSKKTAYDQAVAAAKAILNKQTGSNSDKAAVDRALQQVTSTKDALNGDAKL
                     AEAKAAAKQNLGTLNHITNAQRTDLEGQINQATTVDGVNTVKTNANTLDGAMNSLQGS
                     INDKDATLRNQNYLDADESKRNAYTQAVTAAEGILNKQTGGNTSKADVDNALNAVTRA
                     KAALNGADNLRNAKTSATNTIDGLPNLTQLQKDNLKHQVEQAQNVAGVNGVKDKGNTL
                     NTAMGALRTSIQNDNTTKTSQNYLDASDSNKNNYNTAVNNANGVINATNNPNMDANAI
                     NGMANQVNTTKAALNGAQNLAQAKTNATNTINNAHDLNQKQKDALKTQVNNAQRVSDA
                     NNVQHTATELNSAMTALKAAIADKERTKASGNYVNADQEKRQAYDSKVTNAENIISGT
                     PNATLTVNDVNSAASQVNAAKTALNGDNNLRVAKEHANNTIDGLAQLNNAQKAKLKEQ
                     VQSATTLDGVQTVKNSSQTLNTAMKGLRDSIANEATIKAGQNYTDASPNNRNEYDSAV
                     TAAKAIINQTSNPTMEPNTITQVTSQVTTKEQALNGARNLAQAKTTAKNNLNNLTSIN
                     NAQKDALTRSIDGATTVAGVNQETAKATELNNAMHSLQNGINDETQTKQTQKYLDAEP
                     SKKSAYDQAVNAAKAILTKASGQNVDKAAVEQALQNVNSTKTALNGDAKLNEAKAAAK
                     QTLGTLTHINNAQRTALDNEITQATNVEGVNTVKAKAQQLDGAMGQLETSIRDKDTTL
                     QSQNYQDADDAKRTAYSQAVNAAATILNKTAGGNTPKADVERAMQAVTQANTALNGIQ
                     NLDRAKQAANTAITNASDLNTKQKEALKAQVTSAGRVSAANGVEHTATELNTAMTALK
                     RAIADKAETKASGNYVNADANKRQAYDEKVTAAENIVSGTPTPTLTPADVTNAATQVT
                     NAKTQLNGNHNLEVAKQNANTAIDGLTSLNGPQKAKLKEQVGQATTLPNVQTVRDNAQ
                     TLNTAMKGLRDSIANEATIKAGQNYTDASQNKQTDYNSAVTAAKAIIGQTTSPSMNAQ
                     EINQAKDQVTAKQQALNGQENLRTAQTNAKQHLNGLSDLTDAQKDAVKRQIEGATHVN
                     EVTQAQNNADALNTAMTNLKNGIQDQNTIKQGVNFTDADEAKRNAYTNAVTQAEQILN
                     KAQGPNTSKDGVETALENVQRAKNELNGNQNVANAKTTAKNALNNLTSINNAQKEALK
                     SQIEGATTVAGVNQVSTTASELNTAMSNLQNGINDEAATKAAQKYTDADREKQTAYND
                     AVTAAKTLLDKTAGSNDNKAAVEQALQRVNTAKTALNGDERLNEAKNTAKQQVATMSH
                     LTDAQKANLTSQIESGTTVAGVQGIQANAGTLDQAMNQLRQSIASKDATKSSEDYQDA
                     NADLQNAYNDAVTNAEGIISATNNPEMNPDTINQKASQVNSAKSALNGDEKLAAAKQT
                     AKSDIGRLTDLNNAQRTAANAEVDQAPNLAAVTAAKNKATSLNTAMGNLKHALAEKDN
                     TKRSVNYTDADQPKQQAYDTAVTQAEAITNANGSNANETQVQAALNQLNQAKNDLNGD
                     NKVAQAKESAKRALASYSNLNNAQSTAAISQIDNATTVAGVTAAQNTANELNTAMGQL
                     QNGINDQNTVKQQVNFTDADQGKKDAYTNAVTNAQGILDKAHGQNMTKAQVEAALNQV
                     TTAKNALNGDANVRQAKSDAKANLGTLTHLNNAQKQDLTSQIEGATTVNGVNGVKTKA
                     QDLDGAMQRLQSAIANKDQTKASENYIDADPTKKTAFDNAITQAESYLNKDHGANKDK
                     QAVEQAIQSVTSTENALNGDANLQRAKTEAIQAIDNLTHLNTPQKTALKQQVNAAQRV
                     SGVTDLKNSATSLNNAMDQLKQAIADHDTIVASGNYTNASPDKQGAYTDAYNAAKNIV
                     NGSPNVITNAADVTAATQRVNNAETGLNGDTNLATAKQQAKDALRQMTHLSDAQKQSI
                     TGQIDSATQVTGVQSVKDNATNLDNAMNQLRNSIANKDDVKASQPYVDADRDKQNAYN
                     TAVTNAENIINATSQPTLDPSAVTQAANQVSTNKTALNGAQNLANKKQETTANINQLS
                     HLNNAQKQDLNTQVTNAPNISTVNQVKTKAEQLDQAMERLINGIQDKDQVKQSVNFTD
                     ADPEKQTAYNNAVTAAENIINQANGTNANQSQVEAALSTVTTTKQALNGDRKVTDAKN
                     NANQTLSTLDNLNNAQKGAVTGNINQAHTVAEVTQAIQTAQELNTAMGNLKNSLNDKD
                     TTLGSQNFADADPEKKNAYNEAVHNAENILNKSTGTNVPKDQVEAAMNQVNATKAALN
                     GTQNLEKAKQHANTAIDGLSHLTNAQKEALKQLVQQSTTVAEAQGNEQKANNVDAAMD
                     KLRQSIADNATTKQNQNYTDASQNKKDAYNNAVTTAQGIIDQTTSPTLDPTVINQAAG
                     QVSTTKNALNGNENLEAAKQQASQSLGSLDNLNNAQKQTVTDQINGAHTVDEANQIKQ
                     NAQNLNTAMGNLKQAIADKDATKATVNFTDADQAKQQAYNTAVTNAENIISKANGGNA
                     TQAEVEQAIKQVNAAKQALNGNANVQHAKDEATALINSSNDLNQAQKDALKQQVQNAT
                     TVAGVNNVKQTAQELNNAMTQLKQGIADKEQTKADGNFVNADPDKQNAYNQAVAKAEA
                     LISATPDVVVTPSEITAALNKVTQAKNDLNGNTNLATAKQNVQHAIDQLPNLNQAQRD
                     EYSKQITQATLVPNVNAIQQAATTLNDAMTQLKQGIANKAQIKGSENYHDADTDKQTA
                     YDNAVTKAEELLKQTTNPTMDPNTIQQALTKVNDTNQALNGNQKLADAKQDAKTTLGT
                     LDHLNDAQKQALTTQVEQAPDIATVNNVKQNAQNLNNAMTNLNNALQDKTETLNSINF
                     TDADQAKKDAYTNAVSHAEGILSKANGSNASQTEVEQAMQRVNEAKQALNGNDNVQRA
                     KDAAKQVITNANDLNQAQKDALKQQVDAAQTVANVNTIKQTAQDLNQAMTQLKQGIAD
                     KDQTKANGNFVNADTDKQNAYNNAVAHAEQIISGTPNANVDPQQVAQALQQVNQAKGD
                     LNGNHNLQVAKDNANTAIDQLPNLNQPQKTALKDQVSHAELVTGVNAIKQNADALNNA
                     MGTLKQQIQANSQVPQSVDFTQADQDKQQAYNNAANQAQQIANGIPTPVLTPDTVTQA
                     VTTMNQAKDALNGDEKLAQAKQEALANLDTLRDLNQPQRDALRNQINQAQALATVEQT
                     KQNAQNVNTAMSNLKQGIANKDTVKASENYHDADADKQTAYTNAVSQAEGIINQTTNP
                     TLNPDEITRALTQVTDAKNGLNGEAKLATEKQNAKDAVSGMTHLNDAQKQALKGQIDQ
                     SPEIATVNQVKQTATSLDQAMDQLSQAINDKAQTLADGNYLNADPDKQNAYKQAVAKA
                     EALLNKQSGTNEVQAQVESITNEVNAAKQALNGNDNLANAKQQAKQQLANLTHLNDAQ
                     KQSFESQITQAPLVTDVTTINQKAQTLDHAMELLRNSVADNQTTLASEDYHDATAQRQ
                     NDYNQAVTAANNIINQTTSPTMNPDDVNGATTQVNNTKVALDGDENLAAAKQQANNRL
                     DQLDHLNNAQKQQLQSQITQSSDIAAVNGHKQTAESLNTAMGNLINAIADHQAVEQRG
                     NFINADTDKQTAYNTAVNEAAAMINKQTGQNANQTEVEQAITKVQTTLQALNGDHNLQ
                     VAKTNATQAIDALTSLNDPQKTALKDQVTAATLVTAVHQIEQNANTLNQAMHGLRQSI
                     QDNAATKANSKYINEDQPEQQNYDQAVQAANNIINEQTATLDNNAINQAATTVNTTKA
                     ALHGDVKLQNDKDHAKQTVSQLAHLNNAQKHMEDTLIDSETTRTAVKQDLTEAQALDQ
                     LMDALQQSIADKDATRASSAYVNAEPNKKQSYDEAVQNAESIIAGLNNPTINKGNVSS
                     ATQAVISSKNALDGVERLAQDKQTAGNSLNHLDQLTPAQQQALENQINNATTRGEVAQ
                     KLTEAQALNQAMEALRNSIQDQQQTEAGSKFINEDKPQKDAYQAAVQNAKDLINQTNN
                     PTLDKAQVEQLTQAVNQAKDNLHGDQKLADDKQHAVTDLNQLNGLNNPQRQALESQIN
                     NAATRGEVAQKLAEAKALDQAMQALRNSIQDQQQTESGSKFINEDKPQKDAYQAAVQN
                     AKDLINQTGNPTLDKSQVEQLTQAVTTAKDNLHGDQKLARDQQQAVTTVNALPNLNHA
                     QQQALTDAINAAPTRTEVAQHVQTATELDHAMETLKNKVDQVNTDKAQPNYTEASTDK
                     KEAVDQALQAAESITDPTNGSNANKDAVDQVLTKLQEKENELNGNERVAEAKTQAKQT
                     IDQLTHLNADQIATAKQNIDQATKLQPIAELVDQATQLNQSMDQLQQAVNEHANVEQT
                     VDYTQADSDKQNAYKQAIADAENVLKQNANKQQVDQALQNILNAKQALNGDERVALAK
                     TNGKHDIDQLNALNNAQQDGFKGRIDQSNDLNQIQQIVDEAKALNRAMDQLSQEITDN
                     EGRTKGSTNYVNADTQVKQVYDETVDKAKQALDKSTGQNLTAKQVIKLNDAVTAAKKA
                     LNGEERLNNRKAEALQRLDQLTHLNNAQRQLAIQQINNAETLNKASRAINRATKLDNA
                     MGAVQQYIDEQHLGVISSTNYINADDNLKANYDNAIANAAHELDKVQGNAIAKAEAEQ
                     LKQNIIDAQNALNGDQNLANAKDKANAFVNSLNGLNQQQQDLAHKAINNADTVSDVTD
                     IVNNQIDLNDAMETLKHLVDNEIPNAEQTVNYQNADDNAKTNFDDAKRLANTLLNSDN
                     TNVNDINGAIQAVNDAIHNLNGDQRLQDAKDKAIQSINQALANKLKEIEASNATDQDK
                     LIAKNKAEELANSIINNINKATSNQAVSQVQTAGNHAIEQVHANEIPKAKIDANKDVD
                     KQVQALIDEIDRNPNLTDKEKQALKDRINQILQQGHNGINNAMTKEEIEQAKAQLAQA
                     LQDIKDLVKAKEDAKQDVDKQVQALIDEIDQNPNLTDKEKQALKYRINQILQQGHNDI
                     NNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAIKALANAKRDQINSNPDLTPEQ
                     KAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTHVWEVDEQPAVNEIFE
                     ATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTPSTATISDSLTAKVEV
                     TLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKINEINNSVTLTLEQKEAAI
                     AEVNKLKQQAIDHVNNAPDVHSVEEIQQQEQAHIEQFNPEQFTIEQAKSNAIKSIEDA
                     IQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSIDEISEQLEQFKAQMK
                     AANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTADEKQAAMNQINEIVLETIR
                     DINNAHTLQQVEAALNNGIARISAVQIVTSDRAKQSSSTGNESNSHLTIGYGTANHPF
                     NSSTIGHKKKLDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGISGLLASFWFFIAKRRR
                     KEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEEDVTVEEKDSLNNGESL
                     DKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLKDNEHSPLLFAKRRKDKEEDVETT
                     TSIESKDEDVPLLLAKKKNQKDNQSKDKKSASKNTSKKVAAKKKKKKAKKNKK"
     gene            complement(32778..33179)
                     /locus_tag="O0305_000200"
     CDS             complement(32778..33179)
                     /locus_tag="O0305_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCY0273387.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALEHARELNVQNALLYTDSKLIADSIEAGYVKNANFKPYFDQIEIFEKDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
     gene            complement(33720..34424)
                     /locus_tag="O0305_000201"
     CDS             complement(33720..34424)
                     /locus_tag="O0305_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCY0273388.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            34669..34863
                     /locus_tag="O0305_000202"
     CDS             34669..34863
                     /locus_tag="O0305_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCY0273389.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            34875..35126
                     /locus_tag="O0305_000203"
     CDS             34875..35126
                     /locus_tag="O0305_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCY0273390.1"
                     /translation="MKIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFILVDKENDANWETVLPKVEAVFE"
     gene            35164..36288
                     /locus_tag="O0305_000204"
     CDS             35164..36288
                     /locus_tag="O0305_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor C"
                     /protein_id="HCY0273391.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIQEVMDGVLEEVLTTYPESQLPVLVKHALEENHATNNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPVVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            36304..36741
                     /locus_tag="O0305_000205"
     CDS             36304..36741
                     /locus_tag="O0305_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0273392.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT        14723 a         6517 c         6628 g         9123 t
ORIGIN      
        1 ggactttcag cagcatgtat tatgcctgca actttgtcta ttattaagtc atattacatt
       61 gggaaagata gacaacgcgc tttaagttat tggtcaattg gctcatgggg cggctctggt
      121 gtttgttcat tttttggagg tgcagttgca acgcttttag gttggcgttg gattttcatc
      181 ctatcaatta taatttcatt aattgcactg tttcttatta aaggcacacc tgaaactaaa
      241 tctaaatcga tttctctaaa taaatttgac attaaaggtc tggttctttt agtcattatg
      301 ctcctcagtt taaatatttt aattactaaa ggatcagaat taggtgtaac ctcacttctt
      361 tttattactt tattagctat tgcaattgga tcttttagtt tatttatagt tcttgaaaag
      421 cgtgctacaa atcctttaat cgattttaaa ttatttaaaa ataaagctta cacaggtgca
      481 acagcttcaa actttttgtt aaatggtgtt gcaggaacat taatagtagc caacacattt
      541 gttcaaagag gtttaggata ttcttcattg caagcaggaa gtttatcaat cacttattta
      601 gtaatggtac taattatgat tcgtgttggt gaaaagttac ttcaaacact cggatgcaag
      661 aaaccaatgt taattggaac aggagttctt attgtcggag aatgtctcat ttcattaact
      721 ttcttgccag aaatattcta tgtcatttgt tgtattatag gttatttatt ctttggttta
      781 ggactaggga tatatgctac accatcaaca gatacagcaa ttgcaaatgc accgttagaa
      841 aaagtaggcg ttgctgcagg tatctataaa atggcttctg cattaggtgg agcatttggc
      901 gtcgcattga gtggtgcagt atatgcaatc gtatcaaata tgacaaacat ttatacaggt
      961 gcaatgattg cattatggtt aaatgcaggt atgggaatat tatcattcgt tatcattttg
     1021 ttacttgtgc ctaaacaaaa cgatactcaa ttatgataat tgagaattaa attgaaatca
     1081 tacaagtcgc tacaatatta aacaaaaata taaaccgatt cttatgtgtc attattttaa
     1141 atgaacatag ggattggttt tttattactc ttttacgcta ctttatttat aattattata
     1201 aattgtcaca aattcaattt accttacaat atattttgtg ttattatatt ctggagcata
     1261 aataaattgt tcaacacata gttgtaatgt gtttcaatac tttttggata gattgcgaaa
     1321 ttgtattgaa tcgtcatcgt tttaaatttt taaatgagaa tggaatgagc attacaatac
     1381 acaagcaatc aaaagtaaat acattcacaa cacaacagag acataacaac aagataagga
     1441 gtgaacaata gctgtgaatt atcgtgataa aattcaaaag tttagtattc gtaaatatac
     1501 agttggtaca ttttcaactg tcattgcgac attggtattt ttaggattca atacatcaca
     1561 agcacatgct gctgaaacaa atcaaccagc aagcgtggtt aaacagaaac aacaaagtaa
     1621 taatgaacag actgagaatc gagaatctca agtacaaaat tctcaaaatt cacaaaatgg
     1681 tcaatcatta tctgctactc atgaaaatga gcaaccaaat attagtcaag ctaatttagt
     1741 agatcaaaaa gtagcgcaat catctactac taatgatgaa caaccagcat ctcaaaatgt
     1801 aaatacaaag aaagattcgg caacggctgc gacaacacaa ccagataaag aacaaagtaa
     1861 gcataaacaa aacgaaagtc aatctgctaa taaaaatgga aacgacaata gagcggctca
     1921 tgtagaaaat catgaagcaa atgtagtaac agcttcagat tcatctgata atggtaacgt
     1981 acaacatgac cgaaatgaat tacaagcgtt ttttgatgca aattatcatg attatcgctt
     2041 tattgaccgt gaaaatgcag attctggcac atttaactat gtaaaaggca tttttgataa
     2101 gattaatacg ttattaggca gtaatgatcc aataaacaat aaagacttgc aacttgcata
     2161 caaagaattg gaacaagctg ttgctttaat tcgtacaatg cctcaacgtc aacagactag
     2221 ccgacgttca aatagaattc aaacgcgttc ggttgagtca agagctgcag agcctagatc
     2281 agtatcagac tatcaaaatg caaattcatc atattatgtt gaaaatgcta atgatggttc
     2341 gggctatcct gttggtacat atatcaatgc ttctagtaaa ggggcgccat ataatttacc
     2401 aactacacca tggaatacat tgaaggcctc tgactcaaag gaaattgctc ttatgacagc
     2461 gaaacaaact ggagacgggt accaatgggt tattaagttt aataaaggac atgctccaca
     2521 tcaaaatatg atcttttggt ttgcattacc agcagaccaa gtgccagtag gaagaactga
     2581 ctttgtaaca gttaattcag atggaacaaa tgtacaatgg agtcatggag caggagcagg
     2641 tgcaaataaa ccacttcaac aaatgtggga atatggagta aatgatcctc atcgttcaca
     2701 tgactttaaa ataagaaata gaagtggcca agtaatatat gactggccaa ctgtccatat
     2761 ttattcttta gaagatttat ctagagcgag tgattatttt agtgaagctg gagcgacacc
     2821 tgctactaaa gcttttggta gacaaaattt tgaatatatt aatggtcaaa aacctgctga
     2881 atcaccgggt gttcctaaag tttatacttt catcggtcaa ggtgatgcaa gttatacaat
     2941 ttcatttaaa acacaaggtc caactgttaa taaattgtac tatgcagcag gtgggcgtgc
     3001 tttagagtac aatcaattat ttatgtacag tcaactatac gtcgaatcaa cgcaagacca
     3061 tcaacaacgt cttaatggtt taagacaagt ggttaatcgt acatatcgca taggtacaac
     3121 taaacgtgta gaagtgagtc aaggaaatgt acaaacgaaa aaggtattag aaagtacaaa
     3181 cctaaatata gatgattttg ttgatgatcc tttaagttat gttaagacgc cgagtaataa
     3241 agtgttagga ttttattcga ataatgcaaa tactaatgct tttagaccgg gtggagccca
     3301 acaattaaat gaatatcaat taagtcaatt atttactgat caaaaattac aagaagcagc
     3361 aagaactaga aacccaataa gattaatgat tggtttcgac tatcctgatg cttatggtaa
     3421 tagtgaaact ttagttcctg ttaacttaac ggtattacct gaaatccaac ataatattaa
     3481 attctttaaa aatgacgata ctcaaaatat tgctgaaaaa ccattttcaa aacaagctgg
     3541 gcatccagtt ttctatgtat atgcaggtaa ccaagggaat gcttccgtga atttaggtgg
     3601 tagcgtaaca tctattcaac cattacgtat taatttaaca agtaatgaga attttacaga
     3661 taaagattgg caaattacag gtattccgcg tacattacac attgaaaact cgacaaatag
     3721 acctaataat gccagagaac gcaatattga acttgttggt aacttattac caggggatta
     3781 ctttggaacg atacgttttg gacgtaaaga acaattattc gaaattcgtg ttaaaccaca
     3841 tacaccaaca attacaacga cagctgagca attaagaggt acagcattac aaaaagtgcc
     3901 tgttaatatt tcgggaatac cgttggatcc atcggcattg gtttatttag ttgcaccaac
     3961 aaatcaaact acgaatggtg gtagtgaggc agatcaaata ccatctggtt atacgatact
     4021 tgcgactggt acacctgatg gggtgcataa tacaattact atacgaccgc aagattatgt
     4081 tgtattcata ccacctgtag gtaaacaaat tagagcagta gtttattata ataaagtagt
     4141 tgcatctaat atgagtaatg ctgttactat tttgccagat gacattccac caacaatcaa
     4201 taatcctgtt ggaataaatg ccaaatacta tcgaggcgac gaagtcaact ttacaatggg
     4261 tgtctctgat agacattctg gtataaaaaa tacaactatt acgacattgc cgaatggttg
     4321 gacatcaaat ttaacaaaag cggataagaa taatggctca ttatctatta caggtagagt
     4381 gagtatgaat caggcattta acagtgatat tacatttaaa gtgtcagcga cagacaatgt
     4441 caataatacg acaaatgata gtcaatctaa acatgtttca attcatgtag gtaaaattag
     4501 tgaagatgct catccgattg tattaggaaa tactgagaaa gttgtagtag tcaatccgac
     4561 tgctgtatct aatgatgaaa agcaaagcat aattactgcc tttatgaata aaaaccaaaa
     4621 tataagagga tatttagcat caactgatcc agtaactgtc gataataatg gtaatgtcac
     4681 attacattac cgtgatggct catcgacaac gcttgatgct acaaatgtga tgacatacga
     4741 accagttgtg aaacctgaat accaaactgt caatgctgct aaaacagcaa cggtaacgat
     4801 tgctaaagga caatcattta gtattggtga tattaaacaa tattttactt taagtaatgg
     4861 acaacctatt ccaagtggca catttacaaa tattacatct gatagaacta ttccaactgc
     4921 acaagaagtt agtcaaatga acgcaggcac gcagttatac catataactg ctacaaatgc
     4981 gtatcataaa gatagtgaag acttctatat tagtttgaaa atcatcgatg tgaaacaacc
     5041 agaaggcgat caacgtgtat atcgtacatc aacatatgat ttaactactg atgaaatctc
     5101 aaaagtaaaa caagcattta ttaatgcaaa tagagatgta attacgcttg ccgaaggtga
     5161 tatttcagtt acaaatacac ctaatggtgc taatgtaagt actattacag taaatattaa
     5221 taaaggtcga ttaacgaaat cattcgcgtc aaacctagct aatatgaatt tcttgcgttg
     5281 ggttaatttc ccacaagatt atacagtgac atggacgaat gcaaaaattg caaacagacc
     5341 aacagatggt ggtttatcat ggtctgatga ccataaatct ttaatttatc gttatgatgc
     5401 tacattaggt actcaaatta cgacgaatga tattttaaca atgttaaaag caacaactac
     5461 agtgcctgga ttgcgaaata acattactgg taatgaaaaa tcacaagcag aagctggcgg
     5521 aagacctaac tttagaacga ctggttattc acaatcaaat gcgacaactg atggtcaacg
     5581 tcaatttacg ttgaatggtc aagtgattca agtgttagac atcatcaacc cttcaaacgg
     5641 ttatggtggg caacctgtta caaattcaaa tactcgtgca aaccatagta actcaactgt
     5701 tgttaacgta aacgaaccgg cagctaatgg tgctggcgca tttacaattg accacgttgt
     5761 aaaaagtaat tctacacata atgcaagtga tgcagtttat aaagcacagt tatacttaac
     5821 gccatatggt ccaaaacaat atgttgaaca tttaaatcaa aatacaggaa atactactga
     5881 cgctattaac atttattttg taccaagtga cttagtgaat ccaacaattt cagtaggtaa
     5941 ttacactaat catcaagtgt tctcaggtga aacatttaca aatactatta cagcgaatga
     6001 taactttggt gtgcaatctg taactgtacc aaatacatca caaattacag gtactgttga
     6061 taataaccat caacatgttt ctgcaacggc accaaatgtg acatcagcaa ctaataagac
     6121 aatcaattta ttagcaactg atacaagcgg caatacagct acaacttcgt tcaatgtaac
     6181 agtgaaacct ttgcgtgata aatatcgagt tggtacttca tcaacggctg ctaatcctgt
     6241 gagaattgcc aatatttcga ataatgcgac agtatcacaa gctgatcaaa cgacaattat
     6301 taattcgtta acgtttactg aaacagtacc aaatagaagt tatgcaagag caagtgcgaa
     6361 tgaaatcact agtaaaacag ttagtaatgt cagtcgtact ggaaataatg ccaatgtcac
     6421 agtaactgtt acttatcaag atggaacaac atcaacagtg actgtacctg taaagcatgt
     6481 cattccagaa atcgttgcac attcgcatta cactgtacaa ggccaagact tcccagcagg
     6541 taatggttct agtgcatcag attactttaa gttatctaat ggtagtgaca ttgcagatgc
     6601 aactattaca tgggtaagtg gacaagcgcc aaataaagat aatacacgta ttggtgaaga
     6661 tataactgta actgcacata tcttaattga tggcgaaaca acgccgatta cgaaaacagc
     6721 aacatataaa gtagtaagaa ctgtaccgaa acatgtcttt gaaacagcca gaggtgtttt
     6781 atacccaggt gtttcagata tgtatgatgc gaaacaatat gttaagccag taaataattc
     6841 ttggtcgaca aatgcgcaac atatgaattt ccaatttgtt ggaacatatg gtcctaacaa
     6901 agatgttgta ggcatatcta ctcgtcttat tagagtgaca tatgataata gacaaacaga
     6961 agatttaact attttatcta aagttaaacc tgacccacct agaattgacg caaactctgt
     7021 gacatataaa gcaggtctta caaaccaaga aattaaagtt aataacgtat taaataactc
     7081 gtcagtaaaa ttatttaaag cagataatac accattaaat gtcacaaata ttactcatgg
     7141 tagcggtttt agttcggttg tgacagtaag tgacgcgtta ccaaatggcg gaattaaagc
     7201 aaaatcttca atttcaatga acaatgtgac gtatacgacg caagacgaac atggtcaagt
     7261 tgttacagta acaagaaatg aatctgttga ttcaaatgac agtgcaacag taacagtgac
     7321 accacaatta caagcaacta ctgaaggcgc tgtatttatt aaaggtggcg acggttttga
     7381 tttcggacac gtagaaagat ttattcaaaa cccgccacat ggggcaacgg ttgcatggca
     7441 tgatagtcca gatacatgga agaatacagt cggtaacact cataaaactg cggttgtaac
     7501 attacctaat ggtcaaggta cgcgtaatgt tgaagttcca gtcaaagttt atccagttgc
     7561 taatgcaaag gcgccatcac gtgatgtgaa aggtcaaaat ttgactaatg gaacggatgc
     7621 gatgaactac attacatttg atccaaatac aaacacaaat ggtatcactg cagcatgggc
     7681 aaatagacaa caaccaaata accaacaagc aggcgtgcaa catttaaatg tcgatgtcac
     7741 atatccaggt atttcagctg ctaaacgagt tcctgttact gttaatgtat atcaatttga
     7801 attccctcaa actacttata cgacaacggt tggaggcact ttagcaagtg gtacgcaagc
     7861 atcaggatat gcacatatgc aaaatgctac tggtttacca acagatggat ttacgtataa
     7921 atggaatcgt gatactacag gtacaaatga cgcaaactgg tcagctatga ataaaccgaa
     7981 tgtggctaaa gtcgttaacg caaaatatga cgtcatctat aacggacata cttttgcaac
     8041 atctttacca gcgaaatttg tagtaaaaga tgtgcaacca gcgaaaccaa ctgtgactga
     8101 aacagcggca ggagcgatta caattgcacc tggagcaaac caaacagtga atacacatgc
     8161 cggtaacgta acgacatacg ctgataaatt agttattaaa cgtaatggta acgttgtgac
     8221 gacatttaca cgtcgcaata atacgagtcc atgggtgaaa gaagcatctg cagcaactgt
     8281 agcaggtatt gctggaacta ataatggtat tactgttgca gcaggtactt tcaaccctgc
     8341 tgatacaatt caagttgttg caacgcaagg aagcggagag acagtgagtg atgagcaacg
     8401 tagtgatgat ttcacagttg tcgcaccaca accgaaccaa gcgactacta agatttggca
     8461 aaatggtcat attgatatca cgcctaataa tccatcagga catttaatta atccaactca
     8521 agcaatggat attgcttaca ctgaaaaagt gggtaatggt gcagaacata gtaagacaat
     8581 taatgttgtt cgtggtcaaa ataatcaatg gacaattgcg aataagcctg actatgtaac
     8641 gttagatgca caaactggta aagtgacgtt caatgccaat actataaaac caaattcatc
     8701 aatcacaatt actccgaaag caggtacagg tcactcagta agtagtaatc caagtacatt
     8761 aactgcaccg gcagctcata ctgtcaacac aactgaaatt gtgaaagatt atggttcaaa
     8821 tgtaacagca gctgaaatta acaatgcagt tcaagttgct aataaacgta ctgcaacgat
     8881 taaaaatggc acagcaatgc ctactaattt agctggtggt agcacaacga cgattcctgt
     8941 gacagtaact tacaatgatg gtagtactga agaagtacaa gagtccattt tcacaaaagc
     9001 ggataaacgt gagttaatca cagctaaaaa tcatttagat gatccagtaa gcactgaagg
     9061 taaaaagcca ggtacaatta cgcagtacaa taatgcaatg cataatgcgc aacaacaaat
     9121 caatactgcg aaaacagaag cacaacaagt gattaataat gagcgtgcaa caccacaaca
     9181 agtttctgac gcactaacta aagttcgtgc agcacaaact aagattgatc aagctaaagc
     9241 attacttcaa aataaagaag ataatagcca attagtaacg tctaaaaata acttacaaag
     9301 ttctgtgaac caagtaccat caactgctgg tatgacgcaa caaagtattg ataactataa
     9361 tgcgaagaag cgtgaagcag aaactgaaat aactgcagct caacgtgtta ttgacaatgg
     9421 cgatgcaact gcacaacaaa tttcagatga aaaacatcgt gtcgataacg cattaacagc
     9481 attaaaccaa gcgaaacatg atttaactgc agatacacat gccttagagc aagcagtgca
     9541 acaattgaat cgcacaggta caacgactgg taagaagccg gcaagtatta ctgcttacaa
     9601 taattcgatt cgtgcacttc aaagtgactt aacaagtgct aaaaatagcg ctaatgctat
     9661 tattcaaaag ccaataagaa cagtacaaga agtgcaatct gcgttaacaa atgtaaatcg
     9721 tgtcaatgag cgattaacgc aagcaattaa tcaattagta cctttagctg ataatagtgc
     9781 tttaaaaact gctaagacga aacttgatga agaaatcaat aaatcagtaa ctactgatgg
     9841 tatgacacaa tcatcaatcc aagcatatga aaatgctaaa cgtgcgggtc aaacagaatc
     9901 aacaaatgca caaaatgtta ttaacaatgg tgatgcgact gaccaacaaa ttgccgcaga
     9961 aaaaacaaaa gtagaagaaa aatataatag cttaaaacaa gcaattgctg gattaactcc
    10021 agacttggca ccattacaaa ctgcaaaaac tcagttgcaa aatgatattg atcagccaac
    10081 gagtacgact ggtatgacaa gcgcatctat tgcagcattt aatgaaaaac tttcagcagc
    10141 tagaactaaa attcaagaaa ttgatcgtgt attagcctca catccagatg ttgcgacaat
    10201 acgtcaaaac gtgacagcag cgaatgccgc taaatcagca cttgatcaag cacgtaatgg
    10261 cttaacagtc gataaagcgc ctttagaaaa tgcgaaaaat caactacaac atagtattga
    10321 cacgcaaaca agtacaactg gtatgacaca agactctata aatgcataca atgcgaagtt
    10381 aacagctgca cgtaataaga ttcaacaaat caatcaagta ttagcaggtt caccgactgt
    10441 agaacaaatt aatacaaata cgtctacagc aaatcaagct aaatctgatt tagatcatgc
    10501 acgtcaagct ttaacaccag ataaagcgcc gcttcaaact gcgaaaacgc aattagaaca
    10561 aagcattaat caaccaacgg atacaacagg tatgacgacc gcttcgttaa atgcgtacaa
    10621 ccaaaaatta caagcagcgc gtcaaaagtt aactgaaatt aatcaagtgt tgaatggcaa
    10681 cccaactgtc caaaatatca atgataaagt gacagaggca aaccaagcta aggatcaatt
    10741 aaatacagca cgtcaaggtt taacattaga tagacagcca gcgttaacaa cattacatgg
    10801 tgcatctaac ttaaaccaag cacaacaaaa taatttcacg caacaaatta atgctgctca
    10861 aaatcatgct gcgcttgaaa caattaagtc taacattacg gctttaaata ctgcgatgac
    10921 gaaattaaaa gacagtgttg cggataataa tacaattaaa tcagatcaaa attacactga
    10981 cgcaacacca gctaataaac aagcgtatga taatgcagtt aatgcggcta aaggtgtcat
    11041 tggagaaacg actaatccaa cgatggatgt taacacagtg aaccaaaaag cagcatctgt
    11101 taaatcgacg aaagatgctt tagatggtca acaaaactta caacgtgcga aaacagaagc
    11161 aacaaatgcg attacgcatg caagtgattt aaaccaagca caaaagaatg cattaacaca
    11221 acaagtgaat agtgcacaaa acgtgcaagc agtaaatgat attaaacaaa cgactcaaag
    11281 cttaaatact gctatgacag gtttaaaacg tggcgttgct aatcataacc aagtcgtaca
    11341 aagtgataat tatgtcaacg cagatactaa taagaaaaat gattacaaca atgcatacaa
    11401 ccatgcgaat gacattatta atggtaatgc acaacatcca gttataacac caagtgatgt
    11461 taacaatgct ttatcaaatg tcacaagtaa agaacatgca ttgaatggtg aagctaagtt
    11521 aaatgctgcg aaacaagaag cgaatactgc attaggtcat ttaaacaatt taaataatgc
    11581 acaacgtcaa aacttacaat cgcaaattaa tggtgcgcat caaattgatg cagttaatac
    11641 aattaagcaa aatgcaacaa acttgaatag tgcaatgggt aacttaagac aagctgttgc
    11701 agataaagat caagtgaaac gtacagaaga ttatgcggat gcagatacag ctaaacaaaa
    11761 tgcatataac agtgcagttt caagtgccga aacaatcatt aatcaaacaa caaatccaac
    11821 gatgtctgtt gatgatgtta atcgtgcaac ttcagctgtt acttctaata aaaatgcatt
    11881 aaatggttat gaaaaattag cacaatctaa aacagatgct gcaagagcaa ttgatgcatt
    11941 accacattta aataatgcac aaaaagcaga tgttaaatct aaaattaatg ctgcatcaaa
    12001 tattgctggc gtaaatactg ttaaacaaca aggtacagat ttaaatacag cgatgggtaa
    12061 cttgcaaggt gcaatcaatg atgaacaaac gacgcttaat agtcaaaact atcaagatgc
    12121 gacacctagt aagaaaacag catacacaaa tgcggtacaa gctgcgaaag atattttaaa
    12181 taaatcaaat ggtcaaaata aaacgaaaga tcaagttact gaagcgatga atcaagtgaa
    12241 ttctgctaaa aataacttag atggtacgcg tttattagat caagcgaagc aaacagcaaa
    12301 acagcagtta aataatatga cgcatttaac aactgcacaa aaaacgaatt taacaaacca
    12361 aattaatagt ggtactactg tcgctggtgt tcaaacggtt caatcaaatg ccaatacatt
    12421 agatcaagcc atgaatacgt taagacaaag tattgccaac aaagatgcga ctaaagcaag
    12481 tgaagattac gtagatgcta ataatgataa gcaaacagca tataacaacg cagtagctgc
    12541 tgctgaaacg attattaatg ctaatagtaa tccagaaatg aatccaagta cgattacaca
    12601 aaaagcagag caagtgaata gttctaaaac ggcacttaac ggtgatgaaa acttagctgc
    12661 tgcaaaacaa aatgcgaaaa cgtacttaaa cacattgaca agtattacag atgctcaaaa
    12721 gaacaatttg attagtcaaa ttactagtgc gacaagagtg agtggtgttg atactgtaaa
    12781 acaaaatgcg caacatctag accaagctat ggctagctta cagaatggta ttaacaacga
    12841 atctcaagtg aaatcatctg agaaatatcg tgatgctgat acaaataaac aacaagagta
    12901 tgataatgct attactgcag cgaaagcgat tttaaataaa tcgacaggtc caaacactgc
    12961 gcaaaatgca gttgaagcag cattacaacg tgttaataat gcgaaagatg cattgaatgg
    13021 tgatgcaaaa ttaattgcag ctcaaaacgc agcgaaacaa catttaggta ctttaacgca
    13081 tatcactaca gctcaacgta atgatttaac aaatcaaatt tcacaagcta caaacttagc
    13141 tggtgttgaa tctgttaaac aaaatgcgaa tagtttagat ggtgctatgg gtaacttaca
    13201 aacggctatc aacgataagt caggaacatt agcgagccaa aacttcttgg atgctgatga
    13261 gcaaaaacgt aatgcataca atcaagctgt atcagcagcc gaaaccattt taaataaaca
    13321 aactggaccg aatacagcga aaacagcagt cgaacaagca cttaataatg ttaataatgc
    13381 gaaacatgca ttaaatggta cgcaaaactt aaacaatgcg aaacaagcag cgattacagc
    13441 aatcaatggc gcatctgatt taaatcaaaa acaaaaagat gcattaaaag cacaagctaa
    13501 tggtgctcaa cgcgtatcta atgcacaaga tgtacagcac aatgcgactg aactgaacac
    13561 ggcaatgggc acattaaaac atgccatcgc agataagacg aatacgttag caagcagtaa
    13621 atatgttaat gccgatagca ctaaacaaaa tgcttacaca actaaagtta ccaatgctga
    13681 acatattatt agcggtacgc caacggttgt tacgacacct tcagaagtaa cagctgcagc
    13741 taatcaagta aacagcgcga aacaagaatt aaatggtgac gaaagattac gtgaagcaaa
    13801 acaaaacgcc aatactgcta ttgatgcatt aacacaatta aatacacctc aaaaagctaa
    13861 attaaaagaa caagtgggac aagccaatag attagaagac gtacaaactg ttcaaacaaa
    13921 tggacaagca ttgaacaatg caatgaaagg cttaagagat agtattgcta acgaaacaac
    13981 agtcaaaaca agtcaaaact atacagacgc aagtccgaat aaccaatcaa catataatag
    14041 cgctgtgtca aatgcgaaag gtatcattaa tcaaactaac aatccgacta tggatactag
    14101 tgcgattacc caagctacaa cacaagtgaa taatgctaaa aatggtttaa acggtgctga
    14161 aaacttaaga aatgcacaaa acactgctaa gcaaaactta aatacattat cacacttaac
    14221 aaataaccaa aaatctgcca tctcatcaca aattgatcgt gcaggtcatg tgagtgaggt
    14281 aactgctact aaaaatgcag caactgagtt gaatacgcaa atgggtaact tggaacaagc
    14341 tatccatgat caaaacacag ttaaacaaag tgttaaattt actgatgcag ataaagctaa
    14401 acgtgatgcg tatacaaatg cggtaagcag agctgaagca attctgaata aaacgcaagg
    14461 tgcaaatacg tctaaacaag atgttgaagc ggctattcaa aatgtttcaa gtgctaaaaa
    14521 tgcattgaat ggtgatcaaa acgttacaaa tgcgaagaat gcagctaaaa atgcattaaa
    14581 taacttaacg tcaattaata atgcacaaaa acgtgactta acaactaaaa ttgatcaagc
    14641 aacaactgta gctggtgttg aagctgtatc taatacgagt acacaattga atacagcgat
    14701 ggctaacttg caaaatggta ttaatgataa aacaaataca ctagcaagtg aaaactatca
    14761 tgatgctgat tcagataaga aaactgctta tactcaagcc gttacgaacg cagaaaatat
    14821 tttaaataaa aatagtggat caaatttaga caaaactgcc gttgaaaacg cgttgtcaca
    14881 agttgctaat gcgaaaggtg ccctaaatgg taaccataat ttagagcaag ctaaatcaaa
    14941 tgcaaacact actataaacg gacttcaaca tttaacaact gctcaaaaag ataaattgaa
    15001 acaacaagtg caacaagcac aaaatgttgc aggtgtagat actgttaaat caagtgccaa
    15061 cacattaaat ggtgctatgg gtacgttaag aaatagcata caagataaca cagctacgaa
    15121 aaatggccaa aactatcttg atgctacaga acgtaacaaa acaaactata acaatgctgt
    15181 tgatagtgct aatggtgtca ttaatgcaac aagcaatcca aatatggatg ctaatgcaat
    15241 taaccaaatc gctacacaag tgacatcaac gaaaaatgca ttagatggta cacataattt
    15301 aacgcaagcg aaacaaacag caacaaatgc catcgatggt gctactaact taaataaagc
    15361 gcaaaaagat gcgttaaaag cacaagttac aagtgcgcaa cgtgttgcaa atgtaacaag
    15421 tatccaacaa actgcaaatg aacttaatac agctatgggt caattacaac atggtattga
    15481 tgatgaaaat gcaacaaaac aaactcaaaa atatcgtgac gctgaacaaa gtaagaaaac
    15541 tgcttatgat caagctgtag ctgctgcgaa agcaatttta aataaacaaa caggttcaaa
    15601 ttcagataaa gcagcagttg accgtgcatt acaacaagta acaagtacga aagatgcatt
    15661 gaatggtgat gcaaaactgg cagaagcgaa agcggcagct aaacaaaact taggcacttt
    15721 aaaccatatt acgaatgcac aacgtactga cttagaaggc caaatcaatc aagcgacgac
    15781 tgttgatggc gttaatactg taaaaacaaa tgccaataca ttagacggcg caatgaatag
    15841 cttacaaggt tcaatcaatg ataaagatgc gacattaaga aatcaaaatt atcttgatgc
    15901 ggatgaatca aaacgaaatg catatacgca agctgtcaca gcggctgaag gcattttaaa
    15961 taaacaaact ggtggtaaca catctaaagc agacgttgat aatgcattaa atgcagttac
    16021 aagagcgaaa gcggctttaa atggtgctga caacttaaga aatgcgaaaa cttcagcaac
    16081 aaatacgatt gatggtttac ctaacttaac acaattacaa aaagacaact tgaagcatca
    16141 agttgaacaa gcgcaaaatg tagcaggtgt aaatggtgtt aaagataaag gtaatacgtt
    16201 aaatactgcc atgggtgcat tacgtacaag tatccaaaat gataatacga cgaaaacaag
    16261 tcaaaattat cttgatgcat ctgacagcaa caaaaataat tacaatactg ctgtaaataa
    16321 tgcaaatggt gttattaatg caacgaacaa tccaaatatg gatgctaatg cgattaatgg
    16381 catggcaaat caagtcaata caacaaaagc agcgttaaat ggtgcacaaa acttagctca
    16441 agctaaaaca aatgcgacga acacaattaa caacgcacat gacttaaacc aaaaacaaaa
    16501 agatgcatta aaaacacaag ttaacaatgc acaacgtgta tctgatgcaa ataacgttca
    16561 acacactgca actgaattga acagtgcgat gacagcactt aaagcagcta ttgctgataa
    16621 agaaagaaca aaagcaagcg gtaattatgt caatgctgat caagaaaaac gtcaagcgta
    16681 tgattcaaaa gtgactaacg ctgaaaatat cattagtggt acaccgaatg cgacattaac
    16741 agtcaatgac gtaaatagtg cggcatcaca agtcaatgcg gctaaaacag cattaaatgg
    16801 tgataacaac ttacgtgtag cgaaagagca tgccaacaat acaattgacg gcttagcaca
    16861 attgaataat gcacaaaaag caaaattaaa agaacaagtt caaagtgcaa ctacattaga
    16921 tggtgttcaa actgttaaaa atagttctca aacgttgaat acagcgatga aaggcttaag
    16981 agatagtatt gcgaatgaag caacaattaa agcaggtcaa aactacactg acgcaagtcc
    17041 aaataatcgt aacgagtacg acagtgcagt tactgcagca aaagcaatca ttaatcaaac
    17101 atcgaaccca acgatggaac caaatactat tacgcaagta acatcacaag tgacaactaa
    17161 agaacaggca ttaaatggtg cgcgaaactt agctcaagct aagacaactg cgaaaaacaa
    17221 cttgaataac ttaacatcaa ttaacaatgc acaaaaagat gcgttaacgc gtagcattga
    17281 tggtgcaaca acagtagctg gtgtaaatca agaaactgca aaagcaacag aattaaataa
    17341 cgcaatgcat agtttacaaa atggtatcaa tgatgagaca caaacaaaac aaactcagaa
    17401 atacctagat gcagagccaa gtaagaaatc agcttatgat caagcagtaa atgcagcgaa
    17461 agcaatttta acaaaagcta gtggtcaaaa tgtagacaaa gcagcagttg aacaagcatt
    17521 gcaaaatgtg aacagtacga agacggcgtt gaacggtgat gcgaaattaa atgaagctaa
    17581 agcagctgcg aaacaaacgt taggtacatt aacacacatt aataatgcac aacgtacagc
    17641 gttagacaat gaaattacac aagcaacaaa tgttgaaggt gttaatacag ttaaagccaa
    17701 agcgcaacaa ttagatggtg ctatgggtca attagaaaca tcaattcgtg ataaagacac
    17761 gacgttacaa agtcaaaatt atcaagatgc tgatgatgct aaacgaactg cttattctca
    17821 agcagtaaat gcagcagcaa ctattttaaa taaaacagct ggcggtaata cacctaaagc
    17881 agatgttgaa agagcaatgc aagctgttac acaagcaaat actgcattaa acggtattca
    17941 aaacttagat cgtgcgaaac aggctgctaa cacagcgatt acaaatgctt cggacttaaa
    18001 tacaaaacaa aaagaagcat taaaagcaca agtaacaagt gcaggacgtg tatctgcagc
    18061 aaatggtgtt gaacatactg cgactgaatt aaatactgcg atgacagctt taaagcgtgc
    18121 cattgctgat aaagctgaga caaaagctag tggtaactat gtcaatgctg atgcgaataa
    18181 acgtcaagca tatgatgaaa aagttacagc tgccgaaaat atcgttagtg gtacaccaac
    18241 accaacgtta acaccagcag atgttacaaa tgcagcaacg caagtaacga atgctaagac
    18301 gcagttaaac ggtaatcata atttagaagt agcgaaacaa aatgctaaca ctgcaattga
    18361 tggtttaact tctttaaatg gtccgcaaaa agcaaaactt aaagaacaag tgggtcaagc
    18421 gacgacgttg ccaaatgttc aaactgttcg tgataatgca caaacattaa acactgcaat
    18481 gaaaggtcta cgagatagca ttgcgaatga agcaacgatt aaagcaggtc aaaactacac
    18541 agatgcaagt caaaacaaac aaactgacta caacagtgca gtcactgcag caaaagcaat
    18601 cattggtcaa acaactagtc catcaatgaa tgcgcaagaa attaatcaag cgaaagacca
    18661 agtgacagct aaacaacaag cgttaaacgg tcaagaaaac ttaagaactg cgcaaacaaa
    18721 tgcgaagcaa catttgaacg gcttaagtga cttaactgac gctcaaaaag atgcagtgaa
    18781 acgtcaaatc gaaggtgcaa cgcatgttaa tgaagtaaca caagcacaaa ataatgcgga
    18841 tgcattaaat acagctatga cgaacttgaa aaatggtatt caagatcaga atacgattaa
    18901 gcaaggtgtt aacttcactg atgccgacga agcgaaacgt aatgcatata caaatgcagt
    18961 gacgcaagct gaacaaattt taaataaagc acaaggtcca aatacttcaa aagacggtgt
    19021 cgaaactgcg ttagaaaatg tacaacgtgc taaaaacgaa ttgaacggta atcaaaatgt
    19081 tgcgaacgct aagacaactg cgaaaaatgc attgaataac ctaacatcaa ttaataatgc
    19141 acaaaaagaa gcattgaaat cacaaattga aggtgcgaca acagttgcag gtgtaaatca
    19201 agtgtctaca acggcatctg aattaaatac agcaatgagc aacttacaaa atggtattaa
    19261 tgatgaagca gctacaaaag cagctcagaa atatactgat gcagatagag aaaaacaaac
    19321 tgcatacaat gacgctgtaa cagcagctaa aacgttatta gataaaacag ctggttcaaa
    19381 tgacaataaa gcagctgttg aacaagcatt acaacgtgtg aatactgcta aaacagcatt
    19441 aaatggtgac gagcgattaa atgaagcgaa gaacacagct aaacaacaag tagcgacaat
    19501 gtcacactta actgatgctc aaaaagcaaa cttaacatcg caaatcgaaa gtggtacgac
    19561 tgttgcaggt gttcaaggta ttcaagctaa tgccggtact ttagatcaag caatgaatca
    19621 attaagacaa agtattgctt ctaaagatgc gactaaatca agcgaagatt atcaagacgc
    19681 gaatgcagat ttacaaaatg catacaatga tgcggtaact aatgctgaag gtattattag
    19741 tgcaacgaat aaccctgaaa tgaatcctga tacaattaac caaaaagcga gccaagtgaa
    19801 cagtgcgaag tctgcattga acggtgatga aaaattagca gcagcaaaac aaactgcgaa
    19861 atcagatatc ggtcgtttga cagacttgaa caatgcacaa cgaactgcgg caaatgctga
    19921 agtggatcaa gcaccaaatc ttgcagctgt cacagcggct aaaaataaag caacatcgtt
    19981 aaacacagcg atgggtaatt tgaaacatgc acttgctgaa aaggataata cgaaacgtag
    20041 tgtcaattac acagatgcgg atcaaccaaa acaacaagcg tatgatactg cggttacaca
    20101 agcagaagca attactaatg ctaatggcag caacgcgaat gaaacacaag ttcaagcagc
    20161 actaaaccaa ttgaatcaag ctaaaaatga cttgaatggt gataataaag ttgctcaagc
    20221 aaaagagtca gcgaaacgtg cattagcttc atatagtaac ttgaataatg cgcaatcaac
    20281 tgcagcaatt agtcaaattg acaatgcaac gacagtagca ggcgtaactg ctgcacaaaa
    20341 tactgctaat gaattaaata cagcaatggg tcaacttcaa aatggtataa atgaccaaaa
    20401 cactgttaaa caacaagtga actttacaga tgctgaccaa ggtaagaaag atgcttacac
    20461 aaatgctgtt acgaatgctc aaggtatttt agataaagca cacggtcaaa atatgacgaa
    20521 agcacaagtt gaagctgcat taaatcaagt aacgactgct aagaatgctt taaacggtga
    20581 cgcaaatgta agacaagcaa aatcagatgc gaaagcaaac ttaggtacat taacacactt
    20641 aaataatgca caaaaacaag atttaacatc acaaatcgaa ggcgcaacaa cagtcaacgg
    20701 tgtaaatggt gttaaaacga aagcacaaga cttagatggt gcaatgcaac gattacaatc
    20761 agctatagca aataaagatc aaactaaagc gagcgaaaat tacatcgacg cagatccaac
    20821 taagaaaaca gcatttgata atgctatcac acaagctgaa tcttacttaa ataaagatca
    20881 cggtgcgaat aaagataagc aagctgttga acaagcaatt caaagtgtaa cgtctactga
    20941 aaatgctttg aacggtgacg cgaacttaca acgcgctaaa actgaagcta tacaagctat
    21001 cgataacttg acacatttga atacaccaca aaaaacagca ttaaaacaac aagtgaacgc
    21061 tgcgcaacgt gtatcaggtg taactgatct gaaaaatagt gctacatcac ttaataatgc
    21121 gatggatcaa ttaaaacaag caattgctga tcatgacaca attgtagcta gtggtaatta
    21181 cactaacgcg agtcctgata agcaaggtgc ttatactgat gcatataatg ctgcgaaaaa
    21241 cattgtaaat ggttcgccta atgtgattac aaatgcagca gatgttacag cagcaacaca
    21301 acgtgttaat aatgctgaaa caggtttaaa cggtgataca aacttagcaa ctgcgaagca
    21361 acaagctaaa gatgcattac gtcaaatgac acatttatct gatgcacaaa aacaaagtat
    21421 tactggtcaa attgatagcg cgacacaagt aactggcgtt caaagtgtga aagacaacgc
    21481 gacaaatctt gataatgcaa tgaatcaact tcgaaatagt attgcgaata aagatgatgt
    21541 aaaagcgagt caaccatatg ttgatgcaga tagagataaa caaaatgcat acaatacagc
    21601 agttacaaat gctgaaaata tcattaatgc aacgagtcag ccgacacttg atccatctgc
    21661 agtaacacaa gcagctaatc aagtgagcac taacaaaact gcgcttaatg gtgcacaaaa
    21721 cttagcgaat aaaaagcaag aaacgactgc taacatcaac caattaagtc atttaaataa
    21781 tgctcaaaag caagatttaa atacgcaagt gacaaatgca ccaaatatta gcacagtaaa
    21841 tcaagtgaaa actaaagctg aacaattaga tcaagcaatg gaacgtttaa tcaacggaat
    21901 ccaagacaaa gatcaagtga aacaaagtgt taactttaca gatgcagatc cagaaaaaca
    21961 aacagcatac aacaatgcgg taactgctgc tgaaaatatt attaatcaag caaatggtac
    22021 aaatgcgaac caatcacaag ttgaagcagc actttcaact gtaacaacta cgaaacaagc
    22081 gttgaatggt gatagaaaag taacagatgc taaaaacaat gcaaaccaaa cattatctac
    22141 gttagataac ttaaacaatg cacaaaaagg tgctgttact ggaaacatca atcaagcgca
    22201 cactgtagct gaagtaacgc aagccattca aaccgctcag gaactgaata cagcgatggg
    22261 taacttgaaa aatagcttga atgataaaga cactacactt ggcagtcaaa actttgcaga
    22321 tgcagatcca gagaagaaaa atgcatacaa tgaagcggtt cataatgctg aaaatatttt
    22381 aaataaatct acaggtacga acgtgcctaa agatcaagtt gaagcagcta tgaatcaagt
    22441 gaatgctaca aaagcagcgc ttaatggtac tcaaaacctt gaaaaagcta aacaacacgc
    22501 aaatacagca attgacggtt taagccattt aacaaatgca caaaaagagg cattaaaaca
    22561 attggtacaa caatcgacta ctgttgcaga agcacaaggt aatgagcaaa aagcaaacaa
    22621 tgttgatgca gcaatggaca aattacgtca aagtattgca gataatgcga caacaaaaca
    22681 aaaccaaaat tatactgatg caagtcagaa taaaaaggat gcgtacaata atgctgtcac
    22741 aactgcacaa ggtattattg atcaaactac aagtccaact ttagatccga ctgttatcaa
    22801 tcaagctgct ggacaagtaa gcacaactaa aaatgcatta aatggtaatg aaaacctaga
    22861 ggcagcgaaa caacaagcgt cacaatcatt aggttcatta gataacttaa ataatgcgca
    22921 aaaacaaaca gttactgatc aaattaatgg cgcgcatact gttgatgaag caaatcaaat
    22981 taagcaaaat gcgcaaaact taaatacagc gatgggtaac ttgaaacaag cgatagctga
    23041 caaagatgct acgaaagcga cagttaactt cactgatgca gatcaagcaa aacaacaagc
    23101 atataacact gctgttacaa atgctgaaaa tatcatttca aaagctaatg gcggcaatgc
    23161 aacacaagct gaagttgaac aagcaatcaa acaagttaat gctgcaaaac aagcattaaa
    23221 tggtaatgcc aacgttcaac atgcaaaaga cgaagcaaca gcattaatta atagctctaa
    23281 tgaccttaac caagcacaaa aagacgcatt aaaacaacaa gttcaaaatg caactactgt
    23341 agctggtgta aacaatgtta aacaaacagc acaagagtta aacaatgcta tgacacaatt
    23401 aaaacaaggc attgcagata aagaacaaac aaaagctgat ggtaactttg tcaatgcaga
    23461 tcctgataag caaaatgcat ataatcaagc agtagcgaaa gctgaagcat taattagtgc
    23521 tacgcctgat gttgtcgtta cacctagcga aattactgca gcgttaaata aagttacgca
    23581 agctaaaaat gatttaaatg gtaatacaaa cttagcaacg gcgaaacaaa atgttcaaca
    23641 tgctattgat caattgccaa acttaaacca agcgcaacgt gatgaataca gcaaacaaat
    23701 cacgcaagca acacttgtac caaacgtcaa tgctattcaa caagcggcga caacgcttaa
    23761 tgacgcgatg acacaattga aacaaggtat tgcgaataaa gcacaaatta aaggtagcga
    23821 gaactatcac gatgctgata ctgacaagca aacagcatat gataatgcag taacaaaagc
    23881 agaagaattg ttaaaacaaa caacaaatcc aacaatggat ccaaatacaa ttcaacaagc
    23941 attaactaaa gtgaatgaca caaatcaagc acttaacggt aatcaaaaat tagctgatgc
    24001 caaacaagat gctaagacaa cacttggtac actagatcat ttaaatgatg ctcaaaaaca
    24061 agcgctaaca actcaagttg aacaagcacc agatattgca acagttaata atgttaagca
    24121 aaatgctcaa aatctgaata atgctatgac taacttaaac aatgcattac aagataaaac
    24181 tgagacatta aatagcatta actttactga tgcagatcaa gctaagaaag atgcttatac
    24241 taatgcggtt tcacatgcag aaggtatttt atctaaagca aatggcagca atgcaagtca
    24301 aactgaagtg gaacaagcga tgcaacgtgt gaacgaagcg aaacaagcat tgaatggtaa
    24361 tgacaatgta caacgtgcaa aagatgcagc gaaacaagtg attacaaatg caaatgattt
    24421 aaatcaagcg caaaaagatg cattaaaaca acaagtcgat gctgcgcaaa ctgttgcaaa
    24481 tgtaaacacg attaagcaaa cagcacaaga tttaaatcaa gcaatgacac aattgaaaca
    24541 aggtattgca gataaagacc aaactaaagc aaatggtaac tttgtcaatg ctgatactga
    24601 taagcaaaat gcttacaaca atgcggtagc acatgctgaa caaataatta gtggtacacc
    24661 aaatgcaaac gtggatccac aacaagtggc tcaagcgtta caacaagtga atcaagctaa
    24721 gggtgattta aacggtaacc ataacttaca agttgctaaa gacaatgcaa atacagccat
    24781 tgatcagtta ccaaacttaa atcaaccaca aaaaacagca ttaaaagacc aagtgtcgca
    24841 tgcagaactt gttacaggtg ttaatgctat taagcaaaat gctgatgcgt taaataatgc
    24901 aatgggtaca ttgaaacaac aaattcaagc gaacagtcaa gtaccacagt cagttgactt
    24961 tacacaagcg gatcaagaca aacaacaagc atataacaat gcggctaacc aagcgcaaca
    25021 aatcgcaaat ggcataccaa cacctgtatt gacgcctgat acagtaacac aagcagtgac
    25081 aactatgaat caagcgaaag atgcattaaa cggtgatgaa aaattagcac aagcgaaaca
    25141 agaagcttta gcaaatcttg atacgttacg cgatttaaat caaccacaac gtgatgcatt
    25201 acgtaaccaa atcaatcaag cacaagcgtt agctacagtt gaacaaacta aacaaaatgc
    25261 acaaaatgtg aatacagcaa tgagtaactt gaaacaaggt attgcaaaca aagatactgt
    25321 caaagcaagt gagaactatc atgatgctga tgccgataag caaacagcat atacaaatgc
    25381 agtgtctcaa gcggaaggta ttatcaatca aacgacaaat ccaacgctta acccagatga
    25441 aataacacgt gcattaactc aagtgactga tgctaaaaat ggcttaaacg gtgaagctaa
    25501 attggcaact gaaaagcaaa atgctaaaga tgccgtaagt gggatgacgc atttaaacga
    25561 tgctcaaaaa caagcattaa aaggtcaaat cgatcaatcg cctgaaattg ctacagtgaa
    25621 ccaagttaaa caaacagcaa cgagcctaga tcaagcaatg gatcaattat cacaagctat
    25681 taatgataaa gctcaaacat tagcggacgg taattactta aatgcagatc ctgacaaaca
    25741 aaatgcgtat aaacaggcag tagcaaaagc tgaagcatta ttgaataaac aaagtggtac
    25801 taatgaagta caagcacaag ttgaaagcat cactaatgaa gtgaacgcag cgaaacaagc
    25861 attaaatggt aatgacaatt tggcaaatgc aaaacaacaa gcaaaacaac aattggcgaa
    25921 cttaacacac ttaaatgatg cacaaaaaca atcatttgaa agtcaaatta cacaagcgcc
    25981 acttgttaca gatgtcacta cgattaatca aaaagcacaa acgttagatc atgcgatgga
    26041 attattaaga aatagtgttg cggataatca aacgacatta gcgtctgaag attatcatga
    26101 tgcaactgcg caaagacaaa atgactataa ccaagctgta acagctgcta ataatatcat
    26161 taatcaaact acatcgccta cgatgaatcc agatgatgtt aatggtgcaa cgacacaagt
    26221 gaataatacg aaagttgcat tagatggtga tgaaaacctt gcagcagcta aacaacaagc
    26281 aaacaacaga cttgatcaat tagatcattt gaataatgcg caaaagcaac agttacaatc
    26341 acaaattacg caatcatctg atattgctgc agttaatggt cacaaacaaa cagcagaatc
    26401 tttaaatact gcgatgggta acttaattaa tgcgattgca gatcatcaag ccgttgaaca
    26461 acgtggtaac ttcatcaatg ctgatactga taaacaaact gcttataata cagcggtaaa
    26521 tgaagcagca gcaatgatta acaaacaaac tggtcaaaat gcgaaccaaa cagaagtaga
    26581 acaagctatt actaaagttc aaacaacact tcaagcgtta aatggagacc ataatttaca
    26641 agttgctaaa acaaatgcga cgcaagcaat tgatgcttta acaagcttaa atgatcctca
    26701 aaaaacagca ttaaaagacc aagttacagc tgcaacttta gtaactgcag ttcatcaaat
    26761 tgaacaaaat gcgaatacgc ttaaccaagc aatgcatggt ttaagacaga gcattcaaga
    26821 taacgcagca actaaagcaa atagcaaata tatcaacgaa gatcaaccag agcaacaaaa
    26881 ctatgatcaa gctgttcaag ccgcaaataa tattatcaat gaacaaactg caacattaga
    26941 taataatgcg attaatcaag cagcgacaac tgtgaataca acgaaagcag cattacatgg
    27001 tgatgtgaag ttacaaaatg ataaagatca tgctaagcaa acggttagtc aattagcaca
    27061 tctaaacaat gcacaaaaac atatggaaga tacgttaatt gatagtgaaa caactagaac
    27121 agcagttaag caagatttga ctgaagcaca agcattagat caacttatgg atgcattaca
    27181 acaaagtatt gctgacaaag atgcaacacg tgcgagcagt gcatatgtca atgcagaacc
    27241 gaataaaaaa caatcctatg atgaagcagt tcaaaatgct gagtctatca ttgcaggatt
    27301 aaataatcca actatcaata aaggtaatgt atcaagtgcg actcaagcag taatatcatc
    27361 taaaaatgca ttagatggtg ttgaacgatt agctcaagat aagcaaactg ctggaaattc
    27421 tctaaatcat ttagatcaat taacaccagc tcaacaacaa gcgctagaaa atcaaattaa
    27481 taatgcaaca actcgtggcg aagtagcaca aaaattaact gaagcacaag cacttaacca
    27541 agcaatggaa gctttacgta atagcattca agatcaacag caaacggaag cgggtagcaa
    27601 gtttatcaat gaagataaac cacaaaaaga tgcttaccaa gcagcagttc aaaatgcaaa
    27661 agatttaatt aatcaaacta acaatccaac gcttgataaa gcacaagttg aacaattgac
    27721 acaagctgtt aaccaagcta aagataacct acacggtgat caaaaacttg cagacgataa
    27781 acaacatgcg gttactgatt taaatcaatt aaatggtttg aataatccgc aacgtcaagc
    27841 acttgaaagc caaataaaca acgcagcaac tcgtggcgaa gtagcacaaa aattagctga
    27901 agcaaaagcg cttgatcaag caatgcaagc attacgtaat agtattcaag atcaacaaca
    27961 aacagaatct ggtagcaagt ttatcaatga agataaaccg caaaaagatg cttaccaagc
    28021 agcagttcaa aatgcaaaag atttaattaa ccaaacaggt aatccaacac tcgacaaatc
    28081 acaagtagaa caattgacac aagcagtaac aactgcaaaa gataatctac atggtgatca
    28141 aaaacttgct cgtgatcaac aacaagcagt aacaactgta aatgcattgc caaacttaaa
    28201 tcatgcacaa caacaagcat taactgatgc tataaatgca gcgcctacaa gaacagaggt
    28261 tgcacaacat gttcaaactg ctactgaact tgatcacgcg atggaaacat tgaaaaataa
    28321 agttgatcaa gtgaatacag ataaggctca accaaattac actgaagcgt caactgataa
    28381 aaaagaagca gtagatcaag cgttacaagc tgcagaaagc attacagatc caactaatgg
    28441 ttcaaatgcg aataaagacg ctgtagacca agtattaact aagcttcaag aaaaagaaaa
    28501 tgagttaaat ggtaatgaga gagtcgctga agctaaaaca caagcgaaac aaactattga
    28561 ccaattaaca catttaaatg ctgatcaaat tgcaactgct aaacaaaaca ttgatcaagc
    28621 gacgaaactt caaccaattg ctgaattagt agatcaagca acgcaattga atcaatctat
    28681 ggatcaatta caacaagcag ttaatgaaca tgctaacgtt gagcaaactg tagattacac
    28741 acaagcagat tcagataaac aaaatgctta taaacaagct attgctgatg ctgaaaatgt
    28801 attgaaacaa aatgcgaata agcaacaagt ggatcaagca cttcaaaata ttttaaatgc
    28861 aaaacaagca ttaaatggtg atgaacgtgt agcacttgct aaaacaaatg gtaaacatga
    28921 catcgaccaa ttgaatgcat taaacaatgc tcaacaagat ggatttaaag gtcgcatcga
    28981 tcaatcaaac gatttaaatc aaatccaaca aattgtagat gaggctaagg cacttaatcg
    29041 tgcaatggat caattgtcac aagaaatcac tgacaatgaa ggacgcacga aaggtagcac
    29101 gaactatgtc aatgcagata cacaagtcaa acaagtatat gatgaaacgg ttgataaagc
    29161 gaaacaagca cttgataaat cgactggtca aaacttaact gcaaaacaag ttatcaaatt
    29221 aaatgatgca gtcactgcag ctaagaaagc attaaatggt gaagaaagac ttaataatcg
    29281 taaagctgaa gcattacaaa gattggatca attaacacat ctaaacaatg ctcaaagaca
    29341 attagcaatc caacaaatta ataatgctga aacgctaaat aaagcatctc gagcaattaa
    29401 tagagcaact aaattagata atgcaatggg tgcagtacaa caatatattg acgaacagca
    29461 ccttggtgtt atcagcagca caaattacat caatgcagat gacaatttga aagcaaatta
    29521 tgataatgca attgcgaatg cagcacatga gttagataaa gtgcaaggta atgcaattgc
    29581 aaaagctgaa gcagagcaat tgaaacaaaa tattatcgat gctcaaaatg cattaaatgg
    29641 agaccaaaac cttgcaaatg ccaaagataa agcaaatgcg tttgttaatt cgttaaatgg
    29701 attaaatcaa cagcaacaag atcttgcaca taaagcaatt aacaatgccg atactgtatc
    29761 agatgtaaca gatattgtta ataatcaaat tgacttaaat gatgcaatgg aaacattgaa
    29821 acatttagtt gacaatgaaa ttccaaatgc agagcaaact gtcaattacc aaaacgctga
    29881 cgataatgct aaaacaaact tcgatgatgc caaacgtcta gcaaatacat tgctaaatag
    29941 tgataacaca aatgtgaatg atatcaatgg cgcaatccaa gcagtcaatg atgcaatcca
    30001 taatcttaat ggtgatcaac gactacaaga tgctaaagac aaggcaattc aatctattaa
    30061 tcaagcttta gctaataagc taaaagaaat cgaagcttca aatgcgacgg atcaagacaa
    30121 gcttattgcg aaaaataaag cagaagaatt ggcaaacagc atcatcaaca acattaataa
    30181 agcaacaagt aatcaggctg tatctcaagt tcaaacagca ggcaaccacg cgattgaaca
    30241 agtgcatgcc aatgaaatac caaaagcaaa aattgatgcc aataaagacg ttgataagca
    30301 agttcaagca ttaattgacg aaattgatcg aaatccaaat ctaacagata aggaaaaaca
    30361 agcacttaaa gatcgtatta atcaaatact tcaacaaggt cataacggca ttaacaatgc
    30421 gatgactaaa gaagaaattg aacaagccaa agcacaactt gcgcaagcat tacaagacat
    30481 caaagattta gtgaaagcta aagaagatgc gaaacaagat gttgataaac aagttcaagc
    30541 tttaattgac gaaatcgatc aaaatccaaa tctaacagat aaggaaaaac aagcacttaa
    30601 atatcgaatt aatcaaatac ttcaacaagg tcataacgac attaacaatg cgctgactaa
    30661 agaagaaatt gagcaggcaa aagcacaact tgcacaagca ttgcaagaca tcaaagattt
    30721 agtgaaagct aaagaagatg cgaaaaatgc aataaaagcc ttagctaatg cgaagcgtga
    30781 tcaaatcaat tcaaatccag atttaacacc tgagcaaaaa gcaaaagcgc tcaaagaaat
    30841 tgacgaagct gaaaaacgag cactacaaaa cgttgagaat gctcaaacta tagatcaatt
    30901 aaatcgagga ttaaacttag gtttagatga cattagaaat acacatgtat gggaggttga
    30961 tgaacaacct gctgtaaatg aaatttttga agcaacacct gagcaaatcc tagttaatgg
    31021 tgaactcatt gtacatcgtg atgacatcat tacagaacaa gatattcttg cacacataaa
    31081 cttaattgat cagctttcag cagaagtcat cgatacacca tcaactgcaa cgatttctga
    31141 tagcttaaca gcaaaagttg aagttacatt gcttgatgga tcaaaagtga ttgttaatgt
    31201 tcctgtaaaa gttgtagaaa aagaattgtc agtagtcaaa caacaggcaa ttgaatcaat
    31261 cgaaaatgcg gcacaacaaa agattaatga aatcaataat agtgtgacat taacactgga
    31321 acaaaaagaa gctgcaattg cagaagttaa taagcttaaa caacaagcaa ttgatcatgt
    31381 taacaatgca cctgatgttc attcagttga agaaattcaa caacaagaac aagcgcatat
    31441 tgaacaattt aatccagaac aatttacgat tgaacaagca aaatcaaatg caattaaatc
    31501 gattgaagat gcaattcaac atatgattga tgaaatcaaa gctcgtactg atctaacaga
    31561 taaagagaag caagaagcta ttgctaagtt aaatcaatta aaagaacaag caattcaagc
    31621 gattcaacgt gcgcaaagca tcgatgaaat aagtgagcaa ttggaacaat ttaaagctca
    31681 aatgaaagca gctaatccaa cagcaaaaga actagctaaa cgcaagcaag aagctattag
    31741 tagaattaaa gacttttcaa atgaaaaaat aaatagtatt cgaaatagtg aaattggcac
    31801 agctgatgaa aaacaagcag caatgaatca aattaacgaa attgtgcttg aaacaattag
    31861 agatattaat aatgcgcata cattacagca agttgaggct gcattgaaca atggtattgc
    31921 tcgaatttca gcagtacaaa ttgtaacatc tgatcgtgct aaacaatcgt caagtactgg
    31981 aaatgaatct aatagccatt taacaattgg ttatggaact gcaaatcatc catttaacag
    32041 ttcgactatt ggacataaaa agaaacttga tgaagatgat gacattgatc cacttcatat
    32101 gcgtcacttt agtaataatt tcggtaatgt tattaaaaac gctattggtg tggtgggtat
    32161 ctctggttta ctagctagtt tctggttctt cattgccaaa cgtcgtcgta aagaagatga
    32221 agaggaagaa ttagaaataa gagataataa taaagattca ataaaagaga ctttagacga
    32281 tacaaaacat ttaccacttt tatttgcgaa acgtcgcaga aaagaagatg aagaagatgt
    32341 tactgttgaa gaaaaagatt cgctaaataa tggcgagtca ctcgataaag ttaaacatac
    32401 gccgttcttc ttaccaaaac gtcgtcgtaa agaagatgaa gaagatgtgg aagttacaaa
    32461 tgaaaacaca gatgaaaaag tgttgaaaga taacgaacat tcaccactct tattcgcaaa
    32521 acgacgcaaa gataaagagg aagatgttga aacaacaact agtattgaat ctaaagatga
    32581 ggacgttcct ttattattgg ctaaaaagaa aaatcaaaaa gataaccaat ccaaagacaa
    32641 aaagtcagca tcaaaaaata cttctaaaaa ggtagcagct aaaaagaaga aaaagaaagc
    32701 taagaaaaat aaaaaataat ttgtttcttt gataaataga ggagcaccga ttgacatcac
    32761 atcagtcggt gctcctttta tttattcttt ttaattaatt tatacaatgc ctgttgagcg
    32821 tgttgattcg cttctttgtt ttgttctctc ggtatccatt taacaaataa taaatcaaaa
    32881 tctttttcaa atatttctat ttgatcaaaa taaggtttga aatttgcgtt tttcacataa
    32941 ccagcttcaa tgctatctgc aattagcttt gagtctgtat ataatagtgc gttttgaaca
    33001 tttaattcac gtgcatgttc tagtgcataa atacatgcag cccattctgc agtgtggtta
    33061 tccatttcgc ctaactcatg tgtatatgta taatgctgct catcttcttt gattacaatg
    33121 gcacatgtac ttatgcctgg atttcctttc gtcgcagcat caaaatttat tttcgccata
    33181 ataaacctac tttctattca atacttagtt aaagttacta ttactgtaat acaaaatatg
    33241 ttgggtaatc cattaaaaaa cacgcatcac ttaaataagt aacacgtgtt taaaatactc
    33301 gctgattcaa agatgatttt ctaatacgta gtactgtaat atacttccta aaaaaatcat
    33361 cttcaggctg ggacataaat caatgttcta tgctctacga tgttatattg gcagtagttg
    33421 actgaacgaa aatgcgcttg taacaagctt ttttcaattc tagtcagggg ccccaacaca
    33481 gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg ggtgtgggtc ccaacacaga
    33541 agatgacgaa aagtcagctt acaataatgt gcaagtttgg gatgggcccc aacaaagaga
    33601 aattggattc ccaatttcta cagacaatgc aagttggggt gggacgacga aataaatttt
    33661 gcgaaaatat tatttctgtc ccactccctt aaaacttatt cttttgtgta gtaagtgcgt
    33721 taatagcctt gatctaactt atcaatctta cctttacgat aaaatgattt agcaatatat
    33781 ccaaatggta cattgaaaac tgttgaagct aattttaata cgtacgttgt aataaatatt
    33841 tcaaatacaa ctgtaccagg taaacttccg ataaatgcga tagctacaaa taaagctgta
    33901 tcaattattg agcttaaaaa tgtactacca tatgcacgga tgaaaaacgt tttatcagaa
    33961 ctaaatactt ttttaattag tgaaaagata aatacatcaa tatgttgacc aataatatat
    34021 gcgacgattg agcctaaagc aatgcgtggc acaacatcaa agattgcgtg taatgctttt
    34081 tgtgccatat cttctggtgc aggaataaaa tgtaatgaca attgcataac aataatcata
    34141 attaatgttg atgaaaagcc taaccaaact gctctttttg caactctacg cccataaata
    34201 tcgtttaata tatctgttgc taaataaata gaagcaaaca tgacattacc taaagttgct
    34261 gaaataccaa agatttctac agttttaatc acttgtatgt tggcaatgat tgtgccaatt
    34321 gcaacccatg caattaaacc ttgtttacca aaaaagcgat acataagtac cataagcacg
    34381 aacgttgcaa taaacgtaac tagtcctaaa atttcattat acatattaaa atgtcctcct
    34441 aaattttgat catgcgggtg tttagaaacc gctcaataaa taactaactc attttactga
    34501 aaaatcgttt gcgatgcaat agcatttgaa tataaataca ttttacgtat agaattgtaa
    34561 aaggtttcat tcaaatctta gggtcaaaaa tgttataata tttttatgtc aaatttaaaa
    34621 cagtaacact tatttacaag gttgcaatat tttgaagtaa taaaggaagt gtcgcgtatt
    34681 ttaacttttt cagagcaaaa tgcactcgcg aaaatagatg atttaatgaa tacttattgc
    34741 aatcaatgtc caatcaaaac tcgtctgcgt aaattagagg ggaaaacgaa ggcgcatcat
    34801 ttttgtatca atgagtgttc aatagggaaa gaaataaaac aattaggaaa tgaacttcaa
    34861 taggaggaag tcaaatgaaa attatatcta tatcagaaac accgaaccac aacacaatga
    34921 agattacact tagtgaaagc agagaaggta tgacatcaga tacgtatact aaagttgatg
    34981 attcacagcc agcatttatt aatgacatct taaaggttga aggcgttaaa tcaattttcc
    35041 atgttatgga ctttatttta gtagataaag aaaatgacgc aaattgggaa acagtattgc
    35101 caaaagtaga ggctgtattc gaataaattt ttcatcaact agtattcggg gggaataaag
    35161 tatatggaaa ttttacgtat agagccaaca ccaagtccaa atacaatgaa agttgttttg
    35221 tcatatacaa gagaagacaa gttatctaat acttataaaa aagtagaaga aacacaacca
    35281 agatttataa atcagttgtt atctatagat ggtatcactt ccatttttca tgtcatgaac
    35341 ttcttagctg ttgataaggc accaaaagct gattgggaag tcatattacc tgatattaaa
    35401 gctgcttttt ctgatgcgaa taaggtttta gaatctgtaa atgaacctca aattgacaat
    35461 cattttggtg aaattaaagc tgaattatta acttttaagg gtataccgta tcaaattaag
    35521 ctaacttctg ctgaccaaga attaagagaa caattaccac aaacatatgt tgaccatatg
    35581 actcaagcgc aaacagcaca tgacaatatt gtttttatgc gtaaatggct agatttagga
    35641 aatcgctatg gaaatattca agaagtaatg gatggtgtcc tagaagaagt gctaactacc
    35701 tatccagaat cacagttacc cgtattggta aaacatgctt tagaagaaaa tcacgcaact
    35761 aataattatc atttctatcg acatgtctct ttggatgaat atcatgcaac tgataattgg
    35821 aagactcgat tacgaatgtt aaaccatttt ccaaagccga cttttgaaga tataccgctg
    35881 cttgatttag ctttatctga tgaaaaagta ccggttagac gtcaagcgat tgtattatta
    35941 ggtatgattg aaagtaaaga aattttaccg tatttatata aggggcttcg tgataaaagt
    36001 cctgttgtaa gaagaacagc aggggattgc ataagcgatt tagggtatcc agaggcacta
    36061 ccagaaatgg tgctactatt agatgatcca cagaaaatcg ttaggtggcg tgctgctatg
    36121 tttatctttg atgaaggtaa tgcagagcag cttcccgcac taaaagccca tattaatgac
    36181 aatgcgtttg aagttaaatt acaaattgaa atggccatat ctcgtattga aaacggagat
    36241 gaagcacttg gttcagtatg gaaacaaatg gcaaacagaa caatttaatt ggaggaatta
    36301 aatatgaatg catatgatgc ttatatgaaa gaaattgcgc aacaaatgcg tggcgaatta
    36361 actcaaaatg gttttacaag tttagaaacg agcgaagcag tatcggagta tatgaaccaa
    36421 gtaaatgctg atgacactac ttttgtagtt attaactcta catgcggctg tgcagctgga
    36481 ttagcaagac cagctgcagt agcagttgca acacaaaatg aacatagacc tacaaataca
    36541 gttacagttt ttgctgggca agataaagaa gcaactgcta caatgcgaga attcattcag
    36601 caagcaccat ctagtccttc gtatgcttta ttcaaaggtc aagatttagt ttattttatg
    36661 cctagagaat ttatcgaagg tagagatatt aatgacattg caatggactt aaaggatgcc
    36721 tttgacgaaa attgtaaata gtacacataa ataaatataa aggttaacac attttataat
    36781 attaaaaatg gtgtctgtca ttgaaaatag agaatatagt tgtattctat ttgttaaata
    36841 aagtccgttt ttaccaacta tattttctag aaatttaact gttttaatag gacatcaaac
    36901 ataatattca aatcatgtgt taacctcttt tttaaaattt tttagcatta aagttataga
    36961 tttgggtaaa caattaccaa ttggaaacat a
//
LOCUS       DAKTIP010000006        34309 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAKTIP010000006 DAKTIP010000000
VERSION     DAKTIP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 34309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34309
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.006"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            6..191
                     /locus_tag="O0305_000206"
     CDS             6..191
                     /locus_tag="O0305_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273393.1"
                     /translation="MNIILLDGATQRHPFFICQILAFLFPKFPHINLLLFNPILSTFS
                     NIIIHIDHFHANKKDDN"
     gene            complement(230..481)
                     /locus_tag="O0305_000207"
     CDS             complement(230..481)
                     /locus_tag="O0305_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCY0273394.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWVG
                     GKLFGQWGPELGSIYILPALIGSIILIAIVTLILRAMRK"
     gene            781..1044
                     /locus_tag="O0305_000208"
     CDS             781..1044
                     /locus_tag="O0305_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273395.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(1182..1754)
                     /locus_tag="O0305_000209"
     CDS             complement(1182..1754)
                     /locus_tag="O0305_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0273396.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTMNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(1935..2303)
                     /locus_tag="O0305_000210"
     CDS             complement(1935..2303)
                     /locus_tag="O0305_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0273397.1"
                     /translation="MKTILKTITYLVLTIIGAYAALFILKTIDSHGITDQFNPLVKKD
                     DSYVKTTEVSTRMDDQLRSYSQSVFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(2672..3283)
                     /locus_tag="O0305_000211"
     CDS             complement(2672..3283)
                     /locus_tag="O0305_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0273398.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV"
     gene            3483..4190
                     /locus_tag="O0305_000212"
                     /pseudo
     CDS             3483..4190
                     /locus_tag="O0305_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4229..5167
                     /locus_tag="O0305_000213"
     CDS             4229..5167
                     /locus_tag="O0305_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0273399.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKYLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRLPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(5433..5675)
                     /gene="rpsR"
                     /locus_tag="O0305_000214"
     CDS             complement(5433..5675)
                     /gene="rpsR"
                     /locus_tag="O0305_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCY0273400.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(5727..6230)
                     /gene="ssb"
                     /locus_tag="O0305_000215"
     CDS             complement(5727..6230)
                     /gene="ssb"
                     /locus_tag="O0305_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0273401.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(6251..6547)
                     /locus_tag="O0305_000216"
     CDS             complement(6251..6547)
                     /locus_tag="O0305_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCY0273402.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            6791..6982
                     /locus_tag="O0305_000217"
     CDS             6791..6982
                     /locus_tag="O0305_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273403.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(7068..8165)
                     /gene="ychF"
                     /locus_tag="O0305_000218"
     CDS             complement(7068..8165)
                     /gene="ychF"
                     /locus_tag="O0305_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCY0273404.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(8177..8380)
                     /locus_tag="O0305_000219"
     CDS             complement(8177..8380)
                     /locus_tag="O0305_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCY0273405.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            complement(8410..9291)
                     /locus_tag="O0305_000220"
     CDS             complement(8410..9291)
                     /locus_tag="O0305_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCY0273406.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIVIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEVQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            complement(9448..10293)
                     /locus_tag="O0305_000221"
     CDS             complement(9448..10293)
                     /locus_tag="O0305_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCY0273407.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRSSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGTKVDISIKKSVGKISFEFDSQEDFVRIIEQLN
                     RRYGK"
     misc_binding    10522..10891
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10957..12060
                     /locus_tag="O0305_000222"
     CDS             10957..12060
                     /locus_tag="O0305_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0273408.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            12057..13217
                     /locus_tag="O0305_000223"
     CDS             12057..13217
                     /locus_tag="O0305_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCY0273409.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            13186..15027
                     /locus_tag="O0305_000224"
     CDS             13186..15027
                     /locus_tag="O0305_000224"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCY0273410.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAFLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLYGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            15024..17252
                     /gene="metE"
                     /locus_tag="O0305_000225"
     CDS             15024..17252
                     /gene="metE"
                     /locus_tag="O0305_000225"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCY0273411.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            17295..18047
                     /locus_tag="O0305_000226"
     CDS             17295..18047
                     /locus_tag="O0305_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCY0273412.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIEKFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(18130..19311)
                     /locus_tag="O0305_000227"
     CDS             complement(18130..19311)
                     /locus_tag="O0305_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCY0273413.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            19850..20983
                     /locus_tag="O0305_000228"
     CDS             19850..20983
                     /locus_tag="O0305_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCY0273414.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLTSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISYSLGIL
                     VMSYIVSLLFKGHDHVMIISVSVATFCIMLIYFKNQRLRNKHE"
     gene            complement(21350..21979)
                     /locus_tag="O0305_000229"
     CDS             complement(21350..21979)
                     /locus_tag="O0305_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCY0273415.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(21979..22821)
                     /locus_tag="O0305_000230"
     CDS             complement(21979..22821)
                     /locus_tag="O0305_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273416.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMEILEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(22846..23559)
                     /locus_tag="O0305_000231"
     CDS             complement(22846..23559)
                     /locus_tag="O0305_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCY0273417.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGFGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKQLVDKEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAAANAILFFLIPFFASAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(23556..23759)
                     /locus_tag="O0305_000232"
     CDS             complement(23556..23759)
                     /locus_tag="O0305_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273418.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            24000..24389
                     /locus_tag="O0305_000233"
     CDS             24000..24389
                     /locus_tag="O0305_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCY0273419.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSVKSNHPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            24489..24713
                     /locus_tag="O0305_000234"
     CDS             24489..24713
                     /locus_tag="O0305_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCY0273420.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            24730..25431
                     /gene="tatC"
                     /locus_tag="O0305_000235"
     CDS             24730..25431
                     /gene="tatC"
                     /locus_tag="O0305_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCY0273421.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLIELIRWLFTFGLLFQL
                     PILFMGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(25523..27235)
                     /locus_tag="O0305_000236"
     CDS             complement(25523..27235)
                     /locus_tag="O0305_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCY0273422.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSESNAVKSDVRKLEDAKANDNQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHVDK
                     KSNTSANDQYHVSQLNDALEKAIKAIDDNQLSDADAALTHFIETWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQGAYIMVVAILIF
                     KFKK"
     gene            complement(27216..28445)
                     /gene="efeB"
                     /locus_tag="O0305_000237"
     CDS             complement(27216..28445)
                     /gene="efeB"
                     /locus_tag="O0305_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCY0273423.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(28442..29296)
                     /locus_tag="O0305_000238"
     CDS             complement(28442..29296)
                     /locus_tag="O0305_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273424.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDIIPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(29564..30109)
                     /locus_tag="O0305_000239"
     CDS             complement(29564..30109)
                     /locus_tag="O0305_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273425.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKE
                     FEGHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYDQK"
     gene            complement(30189..30473)
                     /locus_tag="O0305_000240"
     CDS             complement(30189..30473)
                     /locus_tag="O0305_000240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273426.1"
                     /translation="MDINQSNRHYNAYWRGPNKEADEKSAYNNVQVGDGPQHREIGSV
                     ISTDNASWRGPNIENFEKKFYKQCKLAGPQHREFRKEILQAMQVGEGQQI"
     gene            30583..31578
                     /locus_tag="O0305_000241"
     CDS             30583..31578
                     /locus_tag="O0305_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCY0273427.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(31638..32204)
                     /locus_tag="O0305_000242"
     CDS             complement(31638..32204)
                     /locus_tag="O0305_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0273428.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(32218..33279)
                     /locus_tag="O0305_000243"
     CDS             complement(32218..33279)
                     /locus_tag="O0305_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0273429.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNILPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(33293..34219)
                     /locus_tag="O0305_000244"
     CDS             complement(33293..34219)
                     /locus_tag="O0305_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0273430.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLENNGI
                     KSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
BASE COUNT        11030 a         6492 c         5012 g        11775 t
ORIGIN      
        1 tattcatgaa cataatactc ctggatggcg caacccaacg tcatcctttt tttatttgtc
       61 aaatactcgc ttttttattt ccaaaatttc ctcatattaa tttattactt tttaatccta
      121 tcttatctac attttcaaat attataatac atattgacca ttttcacgca aataaaaaag
      181 atgacaactg aatgccatct ttatttcaat ttccaattga atttttttat tatttacgca
      241 tagctcttaa aattaacgtt acgattgcaa ttaagataat tgaaccaatt aatgctggca
      301 agatgtaaat acttcctaat tcaggacccc attgtccgaa tagtttgcca cctacccatg
      361 atccaataat acctgcgata atattgccta aaataccacc tgggatatct ttacccataa
      421 tagcaccagc agcccatcca attaagccac cgacaattaa cattccaata aatccaaaca
      481 taattttcag tctccttttt ctatttattt tgcgttattc taagtagtac cccttattta
      541 caattctaaa acaaattcaa attattttta tccaaatatt tttaaaagta gtaattgaat
      601 atcaatttta ttcaatgtag ctatcgttat ttaaagtctc tgtaccgata atatcatata
      661 catttacatt attttttctg ccgaattcat agcttgatta ttttatgtta taggactaga
      721 atatacacat attattagag catctttgaa ttttaaatca agaagcgagg ttaatgaaca
      781 atgaatatgc atattttata taacttacga actaaacata atttagaaat tgacgaatta
      841 gcacagcaat taaatgagaa atatggtact aaatatgaag cacatcaaat ttgggaatgg
      901 gagaatcatc accatgaacc taaatttaaa gatgccatgc atttagctga cttctttgat
      961 gcaccatatg aaatgttttt agaaagtaag gttaaagaat atcagaaaca tttagaagaa
     1021 gtcgatattc gcatggataa atagatgcaa ataaaccctc acaacacgtt tggcatatat
     1081 cctttcaaat ctatactgga tatattacat tacgttgtga gggtatttta ttaattaata
     1141 tgaattaaga cattttacaa gcgttaatgc aacgaatctt tttagtgatc ttgctcactc
     1201 tttaatactt taccgttctt agcatcaaca gtaacttctt gttttttatt accttttttc
     1261 aaatcgatat tgtaaacaag tttgccatca tctttttcaa gtgaccattc tttaatatca
     1321 ccatcaaatt ctttttgtcc ttctttaatg gcttttttgt aatctatagc atcgctatat
     1381 ttaaagttat cattttcatt cattgtatct tctttttcag tctttttgtt aatcactttt
     1441 ttatttttat cagcaacaag tacttctgac tcttcaccag atttttgttg cgtcacttta
     1501 taagcccatt caccattaga attttcaaat gaaattcctt tcaacttttg gcctttgtaa
     1561 gtttcttcag cttttttcac agcatcttct gggcttgttt taacatcttt taaagcaata
     1621 acatcttttg ttgtattagt gtcttgatta gtatttgact ctgttgattt tgtttcatct
     1681 tttggagtat cattgccaca tgcagtaagc accaccgctg acattgataa cactgctaat
     1741 gattttaatt tcataatatc actctctctt cctatttttg aaactcataa caaaagctta
     1801 tatgctatat agattgtatt accccttgtt tttaatttta ttcataatta ttacaaatat
     1861 ttttaaatta atcgtcatgc gttactttcg ttcgtactcc ttttataaat gaaccatgta
     1921 atataagcat gctattatcg actcagtttg tctaatgctt tctttggtac ttcttccttt
     1981 tcaacttctt caaaagtttc tacatgatga cctttatgtg taattttcaa gtatctatgc
     2041 ggtttaacat caaatgtagc agtatacatt aattgcgtct ctttcccttc tttattaaaa
     2101 acactttgac tataacttcg gagttgatca tccattctag tagacacctc tgtcgtttta
     2161 acataagaat catccttctt tactaatggg ttaaattgat ctgttatacc atgggagtct
     2221 attgttttta aaatgaataa agcagcataa gcgccaatga tagtaagtac aagatatgtt
     2281 attgttttta aaatcgtttt caaaagatca cctctgacaa aatataaaat aggatgtatc
     2341 tgaaaacaac ttacataatt gtaattaaag tgtaagtatt aggagaaaat gatggtattt
     2401 gaatctgaaa gatacgaatc gtttttaaac taagttattt tatagagact ttattatgaa
     2461 attgaaagaa gttaatttta aggttgtcga atgtaaaaac atattgatta accataaaag
     2521 agaaattatt tttgtagatt tcggcataaa cgtcattaaa gaattttcta tatgatggtg
     2581 ctaatcataa caaagaaagc taggcacctt tagataccta actttcaaag tattagcgat
     2641 aaaacactat atatactaat ttagcttaat ttcaaacttg ttcaatgtca ttaacacttt
     2701 tcacaagttt gaaagttaca ttttttatat cggctgtatt aatttttaca ttttccctat
     2761 ctttttgtag ctctttatgc gactctaatg tatatttacc accatctttt gtatttatga
     2821 cgatgttacc tttaggcaaa tgttctccca tatacaagcc ataggaaata tgagcaaatc
     2881 ttatgatatg atccaattct tttaaagtga caacttcttt attaagagta atattcctct
     2941 taggtgtata cactctgcca gcaactgtgt atgttccttc taaatgtata cgaacttggt
     3001 tttcatttaa aggtctctct gaataaaccc aattcctaga tttattagat tttccattta
     3061 ttgtatcctt gctgtatcta tcgtaaagtt ttttaaccaa agctttttct gaattatttg
     3121 gtacttcttc aactttttgt aattgtttat cttgtacact tgaggaattt tgtgttgacg
     3181 cgtctgcttt tggatatatt ccaaatgttc ccccatagat gatacctagc gatagaatag
     3241 attttaatac gattgaattt tttgagtcat attttttgaa catatttaat tacctccttg
     3301 atgtaaagct ttatttgcta caattataaa aataatagac gtgttcatga attaaattca
     3361 tcttaactct tgattaactt taatttgcta ccactctgaa tttaataact ataaatcgtc
     3421 tacacataat tggacaaaat ctaagagaat aaaatttgtt aatttaaaat agcaagcaat
     3481 tcaaagttat atgtgtaata gataaaatag atatccctat agtgatgcgt tactagctaa
     3541 acataataac acattagaag ataatgaagt taaggagtta ctggattgtt tcgactatgt
     3601 aattaagtat aaaaatatcc aacgacaaaa cgtaattata aaatggtaaa agctatggta
     3661 cagtttcaaa ttgctaatga catgcgtatc ggtgagctac ttgcaataaa gagagtaaat
     3721 ataaactatg aagataaaac gctagatatc gacggtaaag ttaattggat aactgaaaaa
     3781 agacgggagc attcggagta aaggagacaa ctgaaagaag taatagctat aaggccacag
     3841 ggctcactac ccaaagcatc gacttactaa gaacacttat gcttggaatg ataagtttat
     3901 tgatagagag tacatattca caaatacgtc tggtagccct atcgactcga acaaaattag
     3961 ccacattatt aaaggggggc gctgatatta gttctattaa gaaacctata acgacgcata
     4021 cattacatca ttcgcatata tctacacttg ctcaattagg aattaactta aaagcaatgc
     4081 aagagcatgt aggtcattca gattataaaa aaaatctaga gatatacaca catgttacta
     4141 atcagatggc gaaagatatg atgaataaat ttgaacgatt ggggagttaa aattggaaaa
     4201 agatgataca ctagcagaaa ttaagcctat gctcaatttt gatgagcaaa tagcgaaatt
     4261 aaaacagatg aatatatttt ttaatattat tgacaccgaa aaagcaaatg aaattcttag
     4321 aaaaaataat tacttcttca aactagctta tttccgaaaa aatttcgaaa aaaagaatgg
     4381 cggctatttc atagaatttg cttatttatc agatttagca actatagata tgaaattaag
     4441 atacacaatg ttgcatttaa ctttagatat tgaacatagt ttaaagtatc tagtcttaaa
     4501 actaataaca gaaaataacc aagaagatgg ttataaaata atagatgagt tcttatgtat
     4561 tgataaatca tatagcaatt caaattttga cacaaattca agaacaccag aagaagttat
     4621 ggaaaccaaa atcaaaaata aaaacgaaat attcaagcat atgaataaac gaggacaact
     4681 acccgagaag ttgaataaat actatcaaaa tccacccgca tgggtttgca ttgaattcat
     4741 gcaactaggt caattcgttt cgtttctcaa cttctattac aagaagtaca atgacgaaga
     4801 attgagagtt gctaatattt taatgccttt agttaaaaat ataagaaaca aatcagctca
     4861 taaccaaccc atcatagcaa atctaaatta tgacagtaga ttacctcaat atttatttga
     4921 aaaagggaat aatataggca tatctagaaa catgttcgga ataaaaaatt tcatagatac
     4981 tttcgctacg ctagaattac ataatcaagt ttgtagtaat gcaattatcc aagcaagata
     5041 tcacgatttg gaccaacttc aaaagcgata taaaagaaac gaaagctatt ataataatgc
     5101 attagctatc aaaagatttt ttatagcttt agataaaatt attgacttca acagaccaaa
     5161 agtataaact atctagtgag gaaagagact tataggtctc gcgagttatt ttaattcgta
     5221 tgcaagaaaa agaagagcta tgcattttat ttaaaatgcg tagttctttt tttatgcatc
     5281 taaattcata ttatttttgc aatataaaca tatctttgtg caaattccga acacaaaaca
     5341 ttcacatcat ccttttttgc cctttttcta taccccaaaa cacaaaaagc cccgtaagcc
     5401 tatgcctacg gggtttgaca ataaattata tattattgtt cttctttaac atatggtaat
     5461 aatgccatat gacgagaacg tttgatagct gtagtcaaca tacgttgata tttagctgaa
     5521 gtaccagtta cacgacgtgg taaaatttta ccgcgttctg agataaaacg ttttaataat
     5581 tcagtgtctt tgtagtcgat atgtgtaata ccatttgctg tgaaatagca tactttttta
     5641 cgacgacgtc cgcctcttct tggtccacct gccatgattg tgtgcctcct ttgataattt
     5701 tttcgcttta atttcgttaa tttttattag aatggtaagt catcatcact tatatcaatc
     5761 ggtccgtttg catttgcaaa tggattatca gattgtttcg tgtttgatga attattgtac
     5821 gaattgtttt gtcctgattg ttgaccaccg aatccttgac cgtaatcttg gaattcattt
     5881 tgttgacgtt ggccaccatt ttgttgcgca tttttaggtt caaggaattg aacgctatca
     5941 cacacaactt cagtaacaaa cacacgacga ccttcttgat tttcataatt acgggattgt
     6001 aagcgaccat ctacaccagc taaactacct ttagataaat agttatttac attatctgct
     6061 tgtcttctaa aaacaacaca gttaataaaa tctgcttcgc gctccccttg agcattcgtg
     6121 aacgtacgat ttactgcaag agtgaatgtc gctacactca cacctgaggg agtggttctg
     6181 tattccggat ctttcgttaa acgacctact aatacaactc tatttagcat ttaaacgccc
     6241 cctctaatta ttacttgtct tcgtcttcac gaataaccat gtaacgaatg atatcgtcac
     6301 tgattttagc tagacgttgg aattcgtcag tagctttgtt gttatcagat ttaacacgta
     6361 cgatgttgta gaagccatct ttgaaatcat tgatttcata agctaggcga cgtttacccc
     6421 agtcttttgc ttctaaaact tctgcacctt cagtagctaa gataccgttg aaacgttcaa
     6481 ctaacgcttt tttagcatct tcctcaatgt ttgggcgtac gatgtacata acttcatatg
     6541 ttctcatttt atatttgcac ctccttgtgg tctatacggc ttatcaatct taaaacagat
     6601 aagcaaggaa taattttcat tactcacaat aaagaattat atcatgcgcc attacttttt
     6661 acaataataa ttcaaactac tcttcatatc atttttgata ttaattcatt tgaaactttc
     6721 tatgatattt ttaaaaatac acttcacaaa agcgaacata tgttctataa tagttgtgag
     6781 gtggtaagga atgaatttaa atattgattg gtctaaagat tttcaagaat tccaagagat
     6841 acttaatagt ggtattcatc ctgaatggct ttattgtgca aaggctaatc ttgttttaga
     6901 gcctgcttat actggcgaag gcaaacaatt tttcagcact caagatatta ttaacgcgag
     6961 taaaattatt ccattctttt aataaattat acgcataatt aaaagaggcg aacaattgat
     7021 tccgcctctt ctacatatat actatttaat taacttcact ctatcgttta tacattaaat
     7081 ctgaaatgaa cgatatcgcc atcttgcata atatattctt taccttctaa tcgttgtctg
     7141 cccgcttctt tagcgccact ttcgccacca tattgtacat agtcgtcata acttgttact
     7201 tcggcacgga taaatccacg ttcaaaatca gtatgaatga taccagcaca ttgaggtgca
     7261 gtcatacctt gtttaaatgt ccaagcacgt acttcttgca caccagcagt aaaatatgtt
     7321 gataatccta ataattcata agttgttcta attaatcgat ctaatcctgg ttcttcgata
     7381 cctaaatctt ctaagaacat ttctttatct tcatcatcta atgtagcaat ttcttcttca
     7441 atttttgcac taataacaat cacttcagag tcttcttgcg ctgcatattc acgaatcgct
     7501 tttactttat cattatcatc atcaccaatt tcatcttcac caacattagc gatataaagc
     7561 atttttttag aagtcagtaa ttgcgcttga ttcacccatt tttgatcttc ttcattaaag
     7621 tcaatactac gagcgggttt accattttct aaagcttctt taatagttgt taaaatacgt
     7681 acttccattt cagcagtctt atctttttga cgtgctaatt tttcaattct aggcaaacgt
     7741 ttctcaacag attctaagtc cgctagtact aattccatat taataacttc aatatcatca
     7801 atagggtcta ctcgaccagc aacatgagtt acgttatcat catcaaatgc acgaacgacc
     7861 tgacaaatcg catctacttc tctaatatgt gataagaatt tattacctaa cccttctccc
     7921 tttgaagcac ctttcacaat accagcgata tctgtaaatt caaatgtagt cggcaatgtc
     7981 tttttaggtt gaaccatttc ttctaattta agtaatctag catctggcac ttctactatc
     8041 cctacattag gatcaatcgt agcgaatgga tagttcgctg ctaaagcacc tgcttttgtt
     8101 attgcattaa ataatgttga tttaccaacg tttggcaatc caacgatacc tgctgttaaa
     8161 gccattaatc attctcctat ctttgtgtat catcatgaga ttcgatgatt tttttaagtt
     8221 ttttatcaaa cgtttgacgt ggaatcataa tacttctttg acaattttca catttaattc
     8281 ttatgtctgc acccattcta ataatcttaa aacggtttgt tccacacgca tgttgttttt
     8341 tcatttctac tatatcattt attccatatt ttgacgccat taaatatgac ctccatgtat
     8401 tataaactac taaacaccgt tttcactatg attataagca gtcataattg gttgaggtgt
     8461 tttaatacct tcttgtaaaa acattttttg tacttctttg cgaataattc gagctccagc
     8521 aaatccttca cctggaattg tttctgcaga tattctcaat attactcttg tatcttcaat
     8581 agcatcaata ccaataacaa ccggatcact aacaaataag taatatttac tacgtaaaga
     8641 agtaaatagt ttgtttagtt ttttatcaac attatcaatg ttttcttcga cagacactgg
     8701 aattttaacg atagctgtac catttgtaat tgagtagttc gttatttcac ccatactact
     8761 atttggtaaa attgttaatt ctcctgaaat tgtattgatt cgtgttgaac gaagtccaat
     8821 tgatttaact gttccctctg ctacagttgt accaccgtta tttatcttaa cataatcacc
     8881 tacatcaaat tggctttcaa atataataaa gaatcctgta attacgtctt taactatagt
     8941 ttgagcacca aaacctactg ctaagcctac gacaccagca ctggcaatta ccccttcaac
     9001 actaatgcca aatttactta aaatcgtcgt aataactata aaccaaacga tatacttcac
     9061 tacattttga acaagagata ttaaagtttt agagcgcttt ttgttactct ttttactttt
     9121 attttgaatc ttaaatccct gttcaatcat tttattcagt attttaataa ctatgagggc
     9181 tacgataata taaataacaa tcatagcgat tttagttgca atgttttcat atgtttctat
     9241 ttttgttaat ggctcaaata gagatgaaat aatattcatg acttgattca tgataagcgt
     9301 tactcccttc tatatgatat atcaatattg tattcatatt tttatatact tccaacctac
     9361 gaaagtctca atatatctag aatcaaatgt tcactgatta caatttattt acacttgcac
     9421 aaagagttat tatataaaat tgtgtaacta tttaccatac ctacgattta attgttcaat
     9481 tattctcaca aaatcttctt gtgaatcaaa ctcaaatgag attttaccaa ccgatttttt
     9541 tattgatata tctactttgg taccatactg ttctcgcaac tgtcgttctt gctgctttat
     9601 aaatttaggc ttagttatat ctactttgtc tgtttccgac tttgacgaaa cattttttaa
     9661 ttcattaaca tggttttcta aatatctgac actccacttt tctttaacaa cccgtttcgc
     9721 taacctaagc atttgttgtt catctttaat tgccaataac gttcgtccat gtgcacttgt
     9781 cagtcgccca tcttttacca tgtcagcaat ctttttcggc aaatgtaata acctcaacat
     9841 attcgctata tacgggcgcg acttactcga tcgtttcgct acttcttgtt gtgtaatttt
     9901 caaatctgtc atcaaacgtt gataactttc agcttcttca atcgcattta agtcttctcg
     9961 ttgtaaattt tcgatgaccg ccagttccat catatcttca tctgttaaat ctttgataat
    10021 cgctgatacg tattttagac cagcaatttt cgaagctcta aaccttcttt caccaactac
    10081 aatgtaataa ccttgaactg tttttctaag cacaattggt tgcaaaattc catattgctt
    10141 aattgaatct gccaaatcat ttaaatgatt ttcatcgaaa gtttttcggg gctgatacgg
    10201 attcggtttg atatccgata tatctatttg cttaatttgt tcatcttttg ttttagtaat
    10261 acgttgatct tcactttttg acaattcact cacatcactt tttatttgat aacaacagtt
    10321 aaatatattt tatgtattct aaatgagtca gacaaccatt aaccacactt tatttaaaaa
    10381 ataattcatt tttcaacttc aagataccaa ttacaatttc acaaacaaat taacttttaa
    10441 atcaaaattt tctgaataat tgttgacaaa tcttttttta ctctgtaagc taattaatat
    10501 cttataacag tttaatgaaa cgtaaacaca ataaagagga aagtaaaaca caccctgctt
    10561 atacagagag tctttagtag ctgagagaag attttgaaag cgtgtttgaa aatggccttg
    10621 gagtgttgat gccaatatga ggtgtctacg ggttcgcccg ttatagcgat acagtattaa
    10681 cattgatgtt aaatggcgta ctggattctt tacgcacgat tttttgttaa taagtatggg
    10741 atagcacatt actatatcct tacttactga ctttaattgt gataattgtt cagtaagcat
    10801 atttactttt aatgcgtact gaataaggtt atttcagcga tggaataaca aataaaggtg
    10861 gtaccgcgaa acataagctt tcgtcctttt tatccgattc attcgggtac gaaggacgga
    10921 agcttttttt attttttctt ataggagggt cttaatatga aggatacaca gttagcccaa
    10981 atcacattaa ccgatgattc aaccggtgct atagcgaatc caatccattt atctactgcc
    11041 tacaagcatc caaaactagg acaatcgaca ggttttgatt atacacgtac taaaaatcct
    11101 acacgctcaa catttgaaac ctgttttgcc aaacttgagc atggtattgc atcattcgct
    11161 acatcaagtg gaatgtcagc cattcaatta atatgtaatc tatttaaacc tcatgatgaa
    11221 attttagttt cattcgattt atatggtggc acatttagat tatttgaatt ttacgagcaa
    11281 caatacgata tcaaatttaa gtacgttgat tttacagatt atgaacaagt tgaaaaagaa
    11341 atcactgata aaacagttgc attattcatt gaaccaatat ctaacccaca aatgattgct
    11401 attgatgtaa agccatacta tcaactttgt aaagctaaag gcttattgtc aattatcgac
    11461 aatacttttt taacacctta tctttcaaca ccactagcag aaggtgctga tatagtctta
    11521 cattcagcca cgaaatatat tggcggacat aacgatgtac tagcaggtgt cgtaaccgtc
    11581 aaagatgaat cactcgcgca acagttgttt gattttcaca acatgactgg cgcaacactt
    11641 tcaccaatag atagttattt gttgttacgt ggacttaaaa ctttgcattt acgcattgag
    11701 cgtgcgcaat caaacgctag aaaacttgct aaaaaatgtc agtcacttca agcaattgac
    11761 gaagtactat atagcgggca aactggcatg cttagtttaa gacttaacaa ggcctatagc
    11821 gtcgctaaat tattagaaaa tttagacatt tgcatttttg cagaaagttt aggaggtact
    11881 gaaacattag tgaccttccc ttacacccaa acacatgttg atatgccaga tgctgaaaaa
    11941 gataaacgtg gcattgatga gtatttaatc cgcttatcac taggtgttga aaattatgaa
    12001 gacatcgaac gcgatatcat ccaagcatta gataaagctc agattggaga gattgtatga
    12061 cactttcaaa agagacagaa gtgatattcg attggcgtag aggtgtggaa tatcattcag
    12121 ctaacccacc actctatgat tcttcaacat tccatcaaac aagtcttggt ggcgatgtta
    12181 aatatgatta tgcacgaagt ggcaacccta accgtgaact tttagaagag aagttagcac
    12241 gattagaaca gggaaaattc gcttttgctt ttgcatcagg tattgctgct atttcagcag
    12301 tacttttgac tttcaaatct ggtgatcatg tcatcttacc cgatgatgta tatggcggta
    12361 cttttcgcct cactgagcaa attttgaatc gatttaatat tgaatttaca accgtagata
    12421 ctacaaagct cgaacaaatc gagggtgcca ttcaatcaaa cacaaaatta atttatatcg
    12481 aaacaccatc gaatccctgt tttaaaatta ctgatatcaa agctgtttct aaaatagccg
    12541 aaaagcatga actactggta gctgtggaca atacatttat gacaccgtta ggtcaatcac
    12601 ctttattact tggcgctgat attgtcattc atagtgctac aaaattttta agtggacata
    12661 gcgatttaat tgctggtgct gtcataacta ataatgaggc aattagtgac gctctttatt
    12721 taatacaaaa tggtacaggc aatatgttat ctgctcagga tagctggaca cttgctaaac
    12781 atttaaaaac ttttccaatc agatttaaac aatctgtcga aaacgctcaa aaaatcgtgt
    12841 catttttaat aaagcaagat gaaatttcag aagtttatta tccgggactc actactgctc
    12901 atttagaaca agctaaaaat ggcggtgccg ttattggctt tcgtttagct gatgagtcta
    12961 aagcacaaca atttgtcgat gcactgacat taccactcgt ttcagtgagt ctgggcggtg
    13021 ttgaaacgat cctttcacat ccagcaacaa tgtctcacgc tgcactacct gaagaagtga
    13081 gacaagaacg tggtatcact ttcggtctat tccgattaag tgttggtctc gaagatcctg
    13141 atgaactcat tgcagacatc aaatacgcat taaaggaggc attcaatgag tcaattcctc
    13201 acacaattga aagataatgt tttagtagct gatggcgcta taggaaccat tttatactct
    13261 gaaggattag acacctgtcc agaagcatat aatcttagcc atccagataa agttgaacgc
    13321 atccatcgtt catatattga agccggtgct gatgtcattc aaaccaatac ttatggtgca
    13381 aattttgaaa agttaaaacg attcggtctt gaagataaag ttaaagcaat acatcaagcc
    13441 gccgttcgca tcgcaaaaaa agcagcaaat aaagatacgt atatattagg cacagttggt
    13501 gggtttagag gtatcaaaca agaggatatc agcttacaaa ctattcttta tcatactgaa
    13561 attcaaatag acaccttaat tgaagaaggc gttgacgcgc tacttttcga aacgtattac
    13621 gacctagaag agttaacaaa tgtcatttca cgaacgagaa agaaatacga cattccaatc
    13681 attgctcaat taaccgcttc aaacacaaat tacttagtta atggtcaggc aatcaatgaa
    13741 ggattaaaac aactcgttca atgtggtgca aacatcgtgg gactcaattg tcatcatggc
    13801 ccgcaccata tgcaagagtc tttcacacat attgaattac cagagcacgc attcttatct
    13861 tgttatccaa atgccagctt attagatatt gaaaatagtg aatttaagta tagtgacaat
    13921 gcacaatatt tcggtcaagt tgctcaaaat ctaattcgcg aaggtgttcg tttaattggt
    13981 ggttgctgtg gtacaacgcc agagcacatc aaatttatta aagaatctat tcagacactt
    14041 aaacctgtta atgacaaaaa agtgattccg ataccaacga aagcactttt caatccatct
    14101 caaaataaag ttagacaatc attaacatct aaggttcaag aacgtccaac cgttattatc
    14161 gaattggata caccgaaaca tttagacacg gatagatttt ttgaaaatat cgctaaactt
    14221 gataaagcta atgtagatgc ggtaacactc gcagataatt cattggcaac tgtcagaatt
    14281 agcaatattg ctgctgctag cttaattaag caatattaca atattgaacc actcgtacat
    14341 attacatgtc gagaccgaaa cttaatcggc ttgcagtccc atttacttgg attatcgctc
    14401 attggcgtta acgaaatatt agccataact ggtgatcctt caaaagttgg tcacttacca
    14461 ggtgcaacca atgtctatga tgttaattct aaaggattaa ctgaactcgc tctaagattt
    14521 aatcaaggta ttaacactga cggtgatgcg ctgaagaaac gtacacactt caacatcgct
    14581 ggcgccttta accctaatgt tcgtaaatta tatggtgccg tcaaaagatt agagaaaaag
    14641 atagaaagcg gaatgtctta ttttataaca caacccgtgt acagcaaaga gaaaatcatt
    14701 gaaatttacc atgccactaa gcacttgaac aaaccatttt tcataggcat tatgcctatc
    14761 gcaagttaca aaaacgcact ctttttgcat aatgaagtgc caggaatcaa gatgtcagat
    14821 gaaattttac aacaatttga agcagttaaa gatgataaag ccaaaacacg agaactaagt
    14881 cttaagcttt caaaggattt aatcgatact gttcatgaat attttaatgg tttatacatt
    14941 atcacaccgt ttcaaaatgt cgaagattca ttagaacttg cagcatactc aaaatctatt
    15001 actgctcaca aggaggcaat attatgacaa caattaaaac atcaaactta ggattcccaa
    15061 gattaggtag aaaaagagaa tggaaaaaag ccatcgaaag ttattgggca aagaagattt
    15121 ctaaagaaga attagatcaa acattaactg atctacataa agaaaattta ttattacaaa
    15181 agtactatca cttagatagc atcccagttg gtgatttctc tttatatgac catatattag
    15241 atacgtcatt attattcaac atcatccctg aacgtttcca aggaagaact attgatgatg
    15301 atttattatt tgatattgct cgcggtaata aagaccatgt tgcaagtgca cttataaaat
    15361 ggtttaacac taactatcat tacatcgtac ctgaatggga caatgttgag cctaaagtga
    15421 gtcgcaatgt attattagat cgctttaaat atgcccaatc tttaaacgtt aacgctcacc
    15481 ctgtcattgt tggtccgatt acttttgtta aattatcaaa aggcggtcac caaacttttg
    15541 aagaaaaagt taaaacgtta ttaccacttt ataaagaagt gtttgaatca ttaattgatg
    15601 caggtgctga gtacattcaa gttgatgagc caatcttagt tacagacgac agcgaaagct
    15661 atgaaaatat tacacgtgaa gcttatgact atttcgaaaa agctggtgtt gctaaaaaat
    15721 tagtcattca aacatacttt gaacgtgcac atcttaaatt cttaagttct ttacctgttg
    15781 gtggtatagg tttagacttt gtccatgata atggctataa ccttaaacaa attgaagctg
    15841 gagattttga taaatcaaaa acattatacg ctggaattat tgatggtcgt aatgtatggg
    15901 caagtgacat tgaagctaaa aaagtcttaa tcgataaatt gttagcacac actaatgaac
    15961 ttgtcattca accatcatct tcattattac atgttccagt atctttagat gatgaaacat
    16021 tagatacaag tgttggcgaa ggcttaagct ttgcaactga aaaattagac gaattagatg
    16081 cattgcgtcg cctatttaat caaaacgata gtgttaaata tgataaatta aaagcacgtt
    16141 atgagcgttt ccaaaatcaa tcattcaaaa acttagatta tgatttcgaa agcgtgcgta
    16201 cttcaagaca atcaccattc gcgcaacgta ttgaacaaca acaaaaacgt ttgaacttac
    16261 cggatttacc aacaacaact attggatcat tcccacaaag ccgagaagtt cgaaaatacc
    16321 gtgcagattg gaagaacaaa cgcattacag acgaagcata tgaaacattc ttaaaaaatg
    16381 aaattgctcg atggattaaa attcaagaag acattggctt agatgtatta gttcacggtg
    16441 aatttgaacg taatgacatg gttgaattct tcggagaaaa attacaaggt ttcttagtaa
    16501 ctaaattcgg ttgggtgcaa tcatatggtt cacgtgccgt aaaaccacca atcatttatg
    16561 gtgatgtaaa atggacagcg cctttaactg ttgatgaaac agtttatgca caaagcttaa
    16621 cagataaacc agttaaaggt atgttaactg gacctgtaac aattctaaac tggtcatttg
    16681 aacgtgttga tttaccacgt aaagtcgttc aagatcaaat tgctttagca atcaacgaag
    16741 aagtattagc acttgaagct gcaggaatca aagttatcca agttgacgaa cctgcattac
    16801 gtgaaggctt accattacgc tctgaatatc acgaacaata tcttaaagat gctgttttat
    16861 catttaaact tgcaacgtct tcagttcgtg atgaaactca aatccataca catatgtgtt
    16921 attctcaatt cggtcaaatc attcatgcta ttcatgactt agatgctgat gttatttcaa
    16981 ttgaaacatc tcgtagccat ggtgatttaa ttaaagactt tgaagatatt aattatgatt
    17041 taggtattgg tctaggtgta tatgatattc atagtccacg tattccaaca aaagaagaaa
    17101 ttactacagc aatcaatcgt tcactacaac aaatcgaccg ctcattattc tgggtaaacc
    17161 ctgactgtgg tttaaaaacg cgaaaagaag aagaagttaa agatgcattg actgtgcttg
    17221 tgaatgctgt taaagctaaa cgccaagaat aatcgcagca tttatcatat aataaactaa
    17281 aaacgggtga ttttatgaca caatatcctt tatggcaaca attaaatact ttaaaacaag
    17341 caacttgggt tgatttaacg catacatttg atccggaaat accacgtttt agtgagtttg
    17401 aaaaaggcga agtttcaact ttattcacgg ttaaagatca tggtttctat gtgcaacgtt
    17461 ggaatattgt aacgcaatat ggtactcaca ttgatgcacc aattcacttt gtcgagaata
    17521 aacgttattt agaagacatt gatttaaaag aattggtatt gccgttaatt gtattagact
    17581 tttcaacaga agtagcgaac aacaatgatt tcatcgtaac acgtgcgcat atagaagcat
    17641 gggaaaaaga gcatggtact attgaacctg gcacttttgt tgcacttcgt accgattggt
    17701 caaaacgttg gcctaatatt gaaaagtttg aaaacaaaga tgcgaacgga caacaacatg
    17761 caccgggttg gggattagat gctttgaagt acttaattga agaacgtcgt gttgaagctg
    17821 ttggtcatga aacattcgat accgatgcat ctgtggacgt tgtaaaaaat ggtgatttag
    17881 ttggcgaacg ttatattctt ggtcaggata aataccaagt cgagttatta actaatttag
    17941 atcaattacc aactcgtggc gccatcattt acgctatcag ccctaaacca aaagatgcac
    18001 ctgggttccc tgttagagca tttgcaatta aaccatcaaa tgactaatat tacccaataa
    18061 atccataaac aaataaaaac aggttatgcc aacatgtaat taatactgtt gtgataacct
    18121 gttttcttat tatttatact ttgatacaac tgcagcgata gcttgaccgc caccaataca
    18181 caatgatgtt aaaccagttt caacttcatc attcagttga tgcaataatg tcactaatac
    18241 tctagcacca gatgcaccaa taggatgtcc taatgcaata gcgccacctt taacattcac
    18301 cttttcagga ggtaatttta attcacgatc aacagctaat gattgtgctg caaatgcttc
    18361 atttaattca aatacatcaa tatcgcttaa ttctttttta ctacgtttca aagccttttc
    18421 tacagcgcca actggtgcaa tacccataat agaaggatct acaccatgac ttccaaagcc
    18481 atcaagcact gccaatggtt cgatatttaa ttctttagct ttgtcttctg acatgactaa
    18541 catcatcgca gcaccatcat tgattcctga tgcattacct gctgtaactg taccgtcttt
    18601 tttgaaagct ggtcttaatc gacttaattt ttcgactgat acattttcac gtacaccttc
    18661 atccttagtg actacgattg gttcaccttt acgttgagga atcgatactg gaactatttc
    18721 actatcaaat tcaccatttt gctgtgcacg tactgctttt tgttgtgagt ttacagcaaa
    18781 tgtatcttgt tcttctcttg aaataccata ttgctctact aaattttcag cagtaatacc
    18841 catatgatat tgattaaata catctgttaa accatcatat accatgctat caaccattga
    18901 ttgatgtccc attttaaaac caaagcgact gttgttgaca agcattggtg attgagacat
    18961 attctccata ccgccagcta gcacgatgtc attttcacca gtcacaatag attgatatgc
    19021 taattgaatc gactttaacc cagaaccaca tactttattc accgtaaatg caggtactgt
    19081 ttctggcaag ccacctttca tagcagcaat tcgtgctgga ttttgtcctt gtcctgcttg
    19141 tagtacgtta ccgatgataa cttcatcaat ctcacttgga ttcaaacccg tctctttaat
    19201 aatatgttct attaaagtcg cacctaaatc ataggctggc acgtctttaa acgcacctcc
    19261 aaaaacgcca ataggtgtcc tgtatgctgc tgctaatacg actctcgtca tgtcatattc
    19321 atctccttat gatatataca attttattac taatacgtct aatgaaccaa tacgccacta
    19381 acatccaatt tatgaaacca attgctacgt atgttacatt ttcattcttg ttatgcattt
    19441 aacaaatcat attatacaat gatgtaattt gtttgggcaa tcggaaacgt aaacggtttc
    19501 actttaacat ttcaccctct taaatatgct ctatcatcct catctatagt acaaaattat
    19561 gctttgattt accacaaatt atcattaatc atttcgcgta atgactctaa ttaaattcta
    19621 tctatcattg catcgctatt cacagctggt ttcaccatca atttctcatc aattttttat
    19681 taccatgacg tctccagtca aatattaaat cgctgatgat tgttaattcc gcaattacct
    19741 tgcagtcacc tctccttcca cttcatcgcc aaatctgccc ctattcacta gaaaaaataa
    19801 atagtatact tagtaaatta attacacatc ttaaaggagt taaaatttta tggttaatgc
    19861 attaacacca aaacatttag ctgaaaagcg tgcaggattc catgagttgt tctttgattt
    19921 aattttcgtc tatgccattc aaaaaattgc acatgtgatt ttaacaacac aaaacggttc
    19981 catttcagct gatttatttt tcaaatacat cgtcatgagt ttgttcttat ggttaatgtg
    20041 gtctcatcaa acatttttta caaatcgatt tggtcaagtt acattcaaag acgtctcgtt
    20101 tatgatgttt aatatgttca tcatggtctt cctatctaac agcctatatc cggactttga
    20161 aaagacattc ttcccattct tcttatgcgt ggcaattatg tatttaagta tcggattaca
    20221 gtatttatta catatacgta cagggttaga ttatggcgat aagcgtacat gtcaagcttt
    20281 tgcgagcgta gcctttgtga tttcattttt atcatttcta tcattagtac taccacaaag
    20341 tatccattac atccctggat ttttaggcgt attcattgca gcgacaggac ttattccatt
    20401 tcagaaatat cttgtattat cacctgttaa tatgatgcat ttagtagaac gtttttcgtt
    20461 actaacaatt attatctttg gcgaggtact ggttggttta gcttcgtcat catttagcat
    20521 tgatcatttt agttacattt atatattcca atttatgatt ttaattagtc ttttcggtgt
    20581 atattggatc attactgaaa attatattaa tcataaatta acatctatcg gtttccgttt
    20641 atcgtatacg catctattaa tcaatatcgc tttaggtgtg attaatgctg caattgtttt
    20701 tagtaataac aataaactaa atgacttgtt tgaaattaat atgatgtata tcagtgtttt
    20761 aatattctat attggattat ggttaatcac gccttatttc cataatgaac tgacaaacgc
    20821 gaagtatatt tcatattcac taggtatact cgttatgtct tatattgtca gccttttatt
    20881 taaaggtcac gaccatgtaa tgattatctc agtatcagtt gcaaccttct gcatcatgct
    20941 gatttatttt aaaaatcaac gtctacgtaa taaacatgaa taaatagtat ttacctcggt
    21001 caaagtattg gaactgaatc caaaagtgtc gaacttcaca ttatactttt gtgacaaact
    21061 tcagttatgc tacatgatcg aggtttttaa atatttccga ggaaatcgtt agcctataca
    21121 atcatcaaat gatacttata aataacatat atcctctaat ccccactatc taaaaggaaa
    21181 taattcccac acaacataac cacctcaaaa caaataaatc aatatagata tgtcacctat
    21241 acttatcccc cacatttcat tccacaccaa aaagaacaga tgacaatcgt aaagatttat
    21301 cacctgctct aaatattatt tcaaatacta tcatcaccaa aatcgacaat tatagttctt
    21361 gtttgttaaa aattttaata gacaatatat aagaaacgct cgttataact ataccaatga
    21421 ccgcatatgc tatgacaact agccatcctt cagtgctaat tttttcaaat tctaatgcag
    21481 atgcaccact aaccatacca tatataaaga aaaagaccac gaaagaaata agtagtagta
    21541 tagatgcaca tatcataatg acattggagt tttcagcgcc aaatttaaat gtcaatggga
    21601 aaatgataga gtatgcccct atgataccaa aactcattaa acctgacata ataccaatca
    21661 tcacactttg ggtcacaatc gtagtcacaa ctaatccaat cattaaactt gcaccgaata
    21721 agattaaata aaaggcaaag tatgacttaa tataatcact acgtttaacc ggtaaagtag
    21781 atacataata catccatctt gagtcttttt catgtttaat attatcagta ataggtgtga
    21841 ttaacataac cccagccatt gccgaactca tcaacggatt aaatactgca aagtatcctg
    21901 cagctataat agcgactata aaataaatat atgtttgctt tctcgttgca taaaaactac
    21961 ttaggaacat acctttcatt atacttcacc tcgcattatg atttttgtag cgtcatcaat
    22021 gtgttttaaa ggctctgcat tttcaatttc tgcataatct ttaactaaaa tttgatattt
    22081 cccctttgac aatctagaag caatgattaa atgcttagga atttcaacat ctttatcttc
    22141 tgtcgtaaca attccatatt gtgctaaaag tatatctttc tgttcagtta aaatcattcg
    22201 tccatctttc ataaacacta atttatcagc taaatgttct atatcttcag aaatatgcga
    22261 tgatattaag atgcctccac cttgtgcgac aaaatcttct agtatttcca ttacttcttc
    22321 tcttccagaa acatccatac ctgcagttgc ttcatctaag attaataact tcacatcatg
    22381 agaaagcgca atcgttaaag ctatcttcat tcgcatccct cttgaaaaag ttttaatttt
    22441 agtttgtagt ggtaactcga aatatttgat taaatcaaag aatttttgac tattccaagt
    22501 catataaata gattgaaata ctttatcaat atctttaata gtcaatttat taggcactct
    22561 caaatcatca aacactacac ctatgtgttc tttatattcg acatcatcca cggtatgttc
    22621 ttctccaaaa aatgtgatac taccgttatc tttatgtcga ttgcctacta gaatattaat
    22681 taatgtcgat ttacctgagc catttttccc aattaatcca actgtctcat tagtagaaat
    22741 cgttaaagat gcatcttgca atttaaaatc cgaatctttg tattgtttgt tcacactatc
    22801 tacgtttaac aatgttgtca tatccatgct cctctttgtt taattttaat aaaaacgtct
    22861 tctcttcaat aagtaactaa aggcgttata tatgaaaata gcaatgataa gtagcaaact
    22921 aaagctttga ttaattccag tagtaattga ataaagccca attactacaa tggctagtat
    22981 caatattgat aagttgatcg catatgtttt aaataatgca attaattcta tatggcgttc
    23041 accttcatcc aatatttcca atcgcttttc agtgtaattc ttatctgcaa tttttggtat
    23101 tctgtcatca aaccttctat taaacagtgt aaattgtgta ttgaaaatag cactagcaaa
    23161 aaatggtatt aaaaagaata gtattgcatt tgctgcagcg tgccctacca caaaaataag
    23221 caatactaaa aaagcaataa ctgtctgcag aatacttaat atacttccat ttaaaacata
    23281 acgattcgca agcaactcat attgatctgt tgcatcgata tctacctctt tatctacaag
    23341 ttgcttatac ttcaatgcac gtctttgatt catcaaaaca atggcttcta ccaatatgat
    23401 aataatcgtc gcaacaatcg atattacaac gacattgtta tatgtcgcaa acgtcaagtt
    23461 ctcaatacca aatccatcaa aattacctaa aattccacct atgatacccc ctacaagtcc
    23521 acccagtaaa agatatccga tatatcttag tattttcatg cctcatcctc ctcaataata
    23581 aaaacagttt ccaccgtttc attgaaaatg cgagcaattt ttattgccgt taatactgat
    23641 ggcataaaat tgtttcgctc aattagcgat atggtttgtc ttgaaacgcc cgcttgttta
    23701 gcaagttgcg tttggtttaa gccatctcgt gctcgtaatt ctttcaatcg attacgcaca
    23761 tcgcatcaac tccttaatta cactgtaaat gatatgcgtc tttttgacaa ctatatttgt
    23821 caaatctaca ccaaaaaata tgattatcca cctatgtatg acattttgaa acaaacacct
    23881 caacgcctac aagtcataat tgtttacttt cgttacacct tcctgcataa ttaacagcat
    23941 tctaatttta gtatgatgca cgcattttca ctaaatcaaa ccattcaaag gagactatta
    24001 tggcatttac attatctgca attcaacaag cacatcaaca atttactggt gttgactttc
    24061 caaaactatt caaagctttt aaagatatgg ggatgactta caatatcgtc aacattcaag
    24121 atggcactgc aacatacgta catcaatcag aagatgatat cgttacgtca tctgtaaaaa
    24181 gtaatcatcc tgttgctcaa aaatcaaaca aaacaatagt tcaagacgtc ttaactagac
    24241 atcaacaagg gcaaacagat tttgaaacat tttgtgatga aatggctgaa gctggcattt
    24301 ataaatggca tatcgatatt caagcgggca cttgtactta tatcgacttg caagaccaag
    24361 ctgttatttc agaattaatc cctcaataaa ctatatttat agcaacattt taattatttc
    24421 ataaaatttt attgataatc attatcgttc ggtataaagt aaatactata tactacttat
    24481 gagtgaggtt gattatcatg ataactaaca cttttatttt aggcatcaca ggcccaacaa
    24541 gtcttgtcgt cattagcatt atcgctttaa ttatttttgg tccgaaaaaa ttaccacaat
    24601 ttggccgtgc catcggttct actttaaaag aatttaaatc tgcaacagaa gatttagata
    24661 aagagtctca cgatacaccc agtaaggaat cgaaacaaca gcgagagcaa tagcactgac
    24721 cacaccttac tggttcactt tagcgaacta cgccatcggt tagtaaaaat tttattgtcg
    24781 ttcgtcatta cggtcatcgt cgtatatgtt tcatcatttt ggtggatgac accattcata
    24841 acgtatatta cccgtgcaca tgtgtcctta catgcatttt cattcacaga aatgatacaa
    24901 atatacgtga tgattatatt tttcatcgcg ttttgtttca tttcaccagt catgttttat
    24961 caattgtggg cattcattgc acctggatta cataataatg aacgtcaatt tatttataaa
    25021 tatagttttt tcagcgtgtt attattctgt gctggcgtcg cttttgcatt ttatgttggc
    25081 tttccaatga tcattcaatt cgcgttaaaa ttatcattaa ctttaaacat ttcgcctgtc
    25141 attggcttta aagcatattt gattgaactc atacgttggt tattcacatt tggtcttcta
    25201 tttcaattac caatattatt tatgggtctt gcaaaattcg gtcttataga taccacatca
    25261 ttgaaacatt atcgtaaata tatttatttc gcttgctttg tattagcaag tatcattgca
    25321 ccacccgatt taacattaaa tatattactc acattgccac tcatattgct ctttgagttc
    25381 agtatgttca tcgttaagtt cacatgccga ggcaaaccgc caacacatta accttatacg
    25441 aatcaatgct gtgattatcg ttatactgca ataccgaata tttccaatat agcaacaaaa
    25501 aaacaggcac tcggcctgtt ttttattttt taaatttaaa gattaagata gcaactacca
    25561 taatataagc accttgtgct atcaatggtt cataacttgg ataaaagccc aaccagttaa
    25621 tcgttgggaa tccttcaata acatgtcttg gcatcgcacc taataattgt aacttttgaa
    25681 tacttacgcc aagcattttg aatcccataa taaaaataaa gatcgacaac actctaaata
    25741 tatagaaaat aggtattaat ttaactataa atctaaataa taatgcaaag atgattaaaa
    25801 taacgatagc taaagcaata ccaataataa aatctttggt cgctagctca cctatcatcc
    25861 ccatatagaa aatgataacc tcgacacctt cacgcaacac agatattaaa ccaatcgtcg
    25921 ctaacaatac caaattacca ttactaatcg cattagcata catattttta atcatgtcat
    25981 tccaacgttt tgcatttgaa cgtttgtgca tccaaacacc aacgataaac attaatatga
    26041 ccgcaacgat acctaatccc gcttccatac tttcacgaag aatgccacta ttccctaaag
    26101 tttctacaaa cgtaattgct aagataatac tcagtacaag tccggcaatt gcaccaccaa
    26161 tcacacttgc agtccctttc ttatctttta cattacgcgt catggtagtc aatgtcatta
    26221 caattaacaa cacttctagc ccttcacgta aaaagataat catcacatcg acgaagctat
    26281 aactatggcc aacaacctct ttaatttggt tatttaaatc tactaaacca tctttcacat
    26341 gtgctttatt atgttcgtct aatacacttt gataatatgg tattttatct tcaattttcg
    26401 tatacaaagc accgtcttta gtttgaattt gaccttcaac atacggccaa gtttctataa
    26461 aatgtgtaag cgcagcatca gcatccgaca attgattgtc gtcgatagct ttaatcgcct
    26521 tctctaacgc atcatttaat tgtgatacat ggtattgatc atttgcagac gtattacttt
    26581 ttttatcgac atgatcaata tttgatttaa aagttgtcca agcatgtgac acttttgccg
    26641 tatctaatgg tgacttatga attgcaattc taagttgtaa taatgcgact tcaatttgtc
    26701 catattgatt tgcgtcataa ttgcgaatca ctgtttcatt acttgtccaa atctgattca
    26761 aactattgtt caaagattct aattccgctt tatttttatc tttaatcgct tttgtcatcg
    26821 cagcatcttt agcatcgact tgctgttgca atagtttaat tttagaaccc gcatctttac
    26881 tagccaattt ctcttcataa gcaattaatg acttcgttaa ttgcgaaagt gtatcttttt
    26941 gattatcatt cgcttttgca tcttcaagct ttctcacatc tgatttgaca gcattacttt
    27001 cactattatc ttcaagcgat aatttcttaa ctgcacttac cacttgctca attgctttct
    27061 gcttattgtc attcgatatc gaattattag aaagtgcaga tttcgcatcc gttatcacac
    27121 tatatacatc actaatactt tgttgttctg ctgcctgact tttcagtaac ccaaagctac
    27181 acaccatagc agcagttatt agcattgcta caaatttagt caaataatgt ttcaccaagg
    27241 tatcctccct tactaacacc tggtaatact aaaaatgaag cagaacctct atgtgtaata
    27301 tattcattta atttatcatt actacctaaa ttattttgta tatcgataaa ttgttttgtc
    27361 gctttttgaa aagcaataaa aagtaagcct gtttcgaagt tacctgtgcg gtcatccgta
    27421 ccatccacat aattaaaggc tctacgtaaa attgacgtat ttgcttcttt cgctagcctc
    27481 gtatgggcat ctttatcaat aatatactcg ccatgactat ctttcgcttt taagtcaatt
    27541 tcatcaaact ctttcccacc tgttaacggt gcaccactat gtcgtttccg accaaatgta
    27601 gcctcttgtt cttccagcgc agtacgatcc cacgtttcaa tgtgtatttg aatacgtctg
    27661 acaacgcaat aagttccatg tttcgcccat ccgtcatcaa taaacacata atctttaagt
    27721 tgattattct tcctaggatt aattgttcca tctttaaatg ccattaaatt tctaggtgtt
    27781 tccttacctt tagcagagat aaaaccagat tgcgcccaac gtaccttaac aatatctcga
    27841 aatggacgaa ctaaattatg aaccgcatga aaggatactt gcgaatcatt tgagcatgct
    27901 tgaatcataa tatcaccatc gctgtaatcg tctattaact gatcattcgg aaaatgcggt
    27961 aaatctttaa aggcatcggg aattttacta gataacccaa ttttcttcat caaagactta
    28021 ctaatcccaa aggtaatcgt taacttgctt gcacctaatc ctatcgattc accggtatct
    28081 actggtggca ttaaaggatt gttactcggc ttgccaactg tatcaccatc catcatacga
    28141 tcagccataa ccgtccactt tttaaacatt gccttaattg catctctatc ttttgacttc
    28201 aaatctaacg caacgaaatt gcatgttttt tgcgtgggtg tggtaatgcc tggttgcact
    28261 ttaccataaa attcatacgc atctttttcc ggatcttctg gtgtattgaa cattgatttg
    28321 aaagaccaca tgctaccaac accacttgcg ccaattgcaa caccggcacc gccaatacct
    28381 aacattttca aaaatgtacg tcttgaaaat tgcgtactat cgttaacttg ttcataattt
    28441 gtcattcagt aatcacagcc attttactta gtggctctcc taatgcatta acagcatccg
    28501 caaatgcttt acggtctttc ttcgatactt tttcaaatga ctcataaccg ccgttgttat
    28561 ctttatattt atccaataat tgattcactt tatcgaagtt catttggata tcatcactta
    28621 attttttatc ttttttctct aaaataggtt taaataagtc ataaattttt tgtgcgcctt
    28681 caacgttcgc tttaaaatca tataaatctg tatgtgaata aatttcttct tcacctgtga
    28741 ttttagaagt tgcaacttca tttaataggt caacagaacc ttgtaacatt aattttggta
    28801 tgatatctaa tgtatcagct ttggcatgca attcttttgc atctttcaat aattgttgtg
    28861 catctttttt agtcacatca tcaattttct tatcttcgta taatgctttt tcaatcttat
    28921 gatatcctga ccattctttt tcctttttct cttctttcat atctgcaaga cgtgcatcaa
    28981 ttttaggatc taaatctcca aatgcttctg caactggttc agagcgttca taatacatac
    29041 gaacttttgg atataacgct tttgcttggg ccatatcatt attttcaatc gctttaacaa
    29101 atttttctgt accttttaaa aattcattta actgttcatc agtatattta tcatatgctt
    29161 ttgttgcttg ttttaattct gctttaacac catcatcttt tttcgatgtc tttgaatcat
    29221 ccttcttact atcgtcgttc ccacatgcag caattaataa cgttgatgct aataatagcg
    29281 ttgttaactt tttcattttg acacctcatt atagaaattc tattatcccc atagatgtgt
    29341 tcttataaat attattttcg cattccctac acgctaaaat aatacattac acttaaaacg
    29401 gctgttttaa agcatcctcc cataaacatc atctagttga taataattat caaaatcatt
    29461 atattgttat aaaataatac tgtcaacaac cttttatttt taaaaattaa ataaaagagc
    29521 gctgccattt taaatgtcat cactcttttg taagtctaat ttgtcatttt tggtcgtatt
    29581 ctgattttaa taaactatag atgtaactcg atgaataaat accatttagt aattcattgt
    29641 cacgtaacat accttctcta gtaaatccca gcctttcagg aatagcccgg cttttttcat
    29701 tattaactgc ggcactaatc tcaattcggt ttaagtcaag ctcttcgaaa caatacttta
    29761 tcaatgcctc aactgcttgt gtcataatcc catgaccctc aaattcttta tctaaatagt
    29821 accctaatga agtttttctg tgcatgtgat taatttcatg taaaccgatg acaccaacta
    29881 gcgttccttc ataccaaatg ccacactgaa atccattacc atcagcaaat tgcaaaagtc
    29941 ctcttttaat aaatgcacgc gtatctgatg gttgctcagt tgcatctacc caaggtaacc
    30001 attccctaag tgaatttctt gaacgattga ctaaattgaa aagcgcttct gtgtcatgag
    30061 cttctaaaat ttttaatgtt atttgttcat tcactttcat tccaaacata ccatcacatc
    30121 ctcattcatt tttcatataa ttctgtaaat tatcattata ataacatatt aatgttacgc
    30181 attgtatctt aaatttgttg tccttcccca acttgcattg cttgtagaat ttcttttcga
    30241 aattctctat gttggggccc cgccaacttg cattgcttgt agaatttctt ttcgaaattc
    30301 tctatgttgg ggccccgcca acttgcattg tctgtagaaa ttacggaccc aatttctcta
    30361 tgttggggcc catccccaac ttgcacatta ttgtaagctg acttttcgtc agcttctttg
    30421 ttggggcccc gccaataagc gttgtagtgc ctattacttt gattgatgtc caagtttcct
    30481 atcacacaac ttaatttaat cactacttta ttattcattc caaatttata tttaaaaata
    30541 cgacaaaaca gtgctaaaat gctattaaag gaggcactgc tcatggcatc attgaaaaat
    30601 aagcatttta tgattgggct atcactaaca tttatagtcg cattatttag ctttttagca
    30661 gctaaattgc caatcctaga taaggttggc gcattaacta tcgcgatatt aatcgctatt
    30721 ctatatcgtc actttagagg atatcctgaa caatatagct caggtattac gttttcatct
    30781 aaatatttat taagatttgc aatcatccta tatggactta aactcaatat attcgatatt
    30841 atcggtcaag gtagtaaact attagccatc gatgtaggtg tcgttatttt cagtattgta
    30901 atgatgctct ttgtcaacaa actgttgcat ggtgacaaaa acatcgcatt attactaggt
    30961 gtcggcacag gcgtttgtgg tgctgctgct attgccgcag tcgctccaat attcaaatca
    31021 cgtgaaaagg atacagctat tagtatcggt atcatcgcat tgattggtac gatattttca
    31081 cttatatata cagctatcta cgctatcttt tcaatgacga caaatgttta tggcgcttgg
    31141 tctggtgtta gtcttcatga aattgcacac gttgtcttag ctggtggctt tggtggtagt
    31201 gatgcactta aaattgcact tcttggtaaa cttggtagag tattcttact gattccatta
    31261 accatcgtac ttattttaat tatgcgtttc cgttcatcag aatcatctag caagggtcga
    31321 ataagcattc catacttttt aattggattt gtcattatgg ccttagtgaa tacatatgta
    31381 actattccgt cagcattgct aaatatttta aatactgtat caacaatttg cttattaatg
    31441 gccatggttg ctttagggtt aaatgttgca tttaaagatc ttaaaaatcg cgcacttaaa
    31501 ccgcttatga caatcattat tacctcaata tgcttgtcat cacttgcctt tatcgtcgtt
    31561 cattggctat atagttaata tgaaaagacg ctcaaacact tacaaattta gtgtgccgag
    31621 cgtcttattt ttatacttta aaagtcgtat tggttgtttt cttcttcaaa cacttgttgt
    31681 tgtactttgg cagttcgcat tgtggattgt gccaacgctt ttaaccgaaa tacaacatca
    31741 tcattgacaa tttgttgatt tgaaaaatct ttctcttcaa taaatacata cgtttgcatc
    31801 gtatgtgctt tcatgtaact caatattggt tttaaatgca tttcaggaat taaataatgt
    31861 ttactagaac ctgctgtcgc tacaagtcct attaccttgt cacgaaacgc attgactgga
    31921 agtagatcaa acacattttt caaagcacca gggatggaag cttgaaaaat tggaaaacca
    31981 ataaaaatca catcagcctg cattaacgac gtcgttaatt tatatacatc tcctgtagta
    32041 tctagataat tctttccaac gctaaattct aattcaagtt ctcgtaaatc catcaactct
    32101 atttgatgtc cctcattgat gacttctagt tcatttttta aatcatccat agcaattctc
    32161 gttttagaac ctactgtgga acctgacaat aatacaatat tcatgatgac gcccctccta
    32221 ttttgataaa tgctttttaa tcgccggtat aatgtcgttg ccaattaact caatattctt
    32281 cataacattt tcaaatggca caccgccaaa atcaagctgt gccataaaac gttgatgacc
    32341 atacaactcg tgttgataca atatcttttc aataatttgt tgcgggcttc caaccattag
    32401 cgcttctcga taatctgacg aattagcaaa ttgctgtttc ggataaccaa caccacgaat
    32461 aaatgacatc cctgtattca aatgtggata aaattctctc atagcatcct gagttgtttc
    32521 agctgtataa aacagactcg ctgtacttac tggtaaagac ttaggcgaag catcgaaacc
    32581 tgcttcagtt gccgcttgac gataagcatc tatagaacct ttaaagttca ttgctgggcc
    32641 accaagggtt gtaatcatca ttggcacacc ttgttttccc gctttaatag cacttgcagg
    32701 tggaccacca acagcacgcc atattggcaa tatattatcg attggtcttg ggaatatttt
    32761 catatttcta agttctggac gatattttcc agaccaagta atacgctcag ttttatttaa
    32821 ctctaaaagt aaacctaatt tttcttcaaa caattcatca tagtctttta aatcatagcc
    32881 aaacaagtca aaaatacctg ttcttgatgc tctgccagct acaatttcgg ctctaccatg
    32941 agaaatcaaa tctaatgtcg cgaagtcttc aaatactctt acaggatctg ttgcactaat
    33001 aatcgttgaa gaacttgaaa ctttaatatg ctttgtcgct tgggcaattg cacctaacac
    33061 aaccgtatgt gcctgtgttg taaaatgctc ctgatgactt tcaccaactg caaaaacatc
    33121 aatacctgct tcatctgcta atttacttgc ttcaataatt tcattaatac gttgctcata
    33181 actaactttt tcacctttca atggattcaa taaatgatca cctaaggaat acaaaccaaa
    33241 ctcaagaggc gtatttttat tcatttctaa tttggccatt aaacattctc ctttatattt
    33301 cgtgtaactt actttcaatt tgttcacgtt ctgcttctaa aaagtcaggc aagttcaatt
    33361 gacttcctaa ttgttcaaca ggtgtatcaa tagtaaatcc aggcgcttca gtcgcaaatt
    33421 catacataat tgaattatgg cgatagtata atgatttaaa gaaatagcga tcaattatac
    33481 ctgaattact ttgtggttgt tgttgtaatt tcttgtaaat tgcatctaag tcactcattt
    33541 gtggtgtatt aactgcaata tgatggatat aacctcgtcc aggtctttca cgttgtccct
    33601 gttgctcaat aactacaaaa tccgaatata aaccttgtgg tgctaatgtc agcacatcga
    33661 atgatttatt atctcttttg cgataaccta agacattttc caaaaattct accgtacgcg
    33721 ctgcatttct aactcttaat tcgactggcc ctattcctaa aatttgatat gcttgaggta
    33781 tttcactata agcgttatgt tgccattgat gtggtacttc aaaactatca ttaacaagca
    33841 atatgatttc taagtcgtct tcatctttaa agacaacacc ttcttgtcca agatagtaca
    33901 aacgttcaga cttaatacca ttattctcaa gacgtttttc aaaataatga agtgccgctt
    33961 gatttggtac taataatgaa aatcgataaa tcgtttcagt acctggctgc ttatgacccg
    34021 cattgggaat ttcaaaaaag cttaaaattg ttccggctgt acctacttcg tccccataaa
    34081 acaaatgata cattgaagga ttgtcttgat taaccgactt ttcaactaat cgtaatccaa
    34141 ggacatttgt gtaaaaatcc ttattacgtt ttgcatcttt tgtatacatg gatatgtgat
    34201 gatgccctac tatattcata ctatccctcc tattagtata aattttatac cagcttacat
    34261 aaaaaagata gttatatgcc atttacccct tgcaaataaa aatatgttt
//
LOCUS       DAKTIP010000007        33519 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAKTIP010000007 DAKTIP010000000
VERSION     DAKTIP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 33519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33519
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.007"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            92..442
                     /gene="sarV"
                     /locus_tag="O0305_000245"
     CDS             92..442
                     /gene="sarV"
                     /locus_tag="O0305_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCY0273431.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(544..1755)
                     /locus_tag="O0305_000246"
     CDS             complement(544..1755)
                     /locus_tag="O0305_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273432.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFLVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1748..2188)
                     /locus_tag="O0305_000247"
     CDS             complement(1748..2188)
                     /locus_tag="O0305_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCY0273433.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLREAHND"
     gene            2350..3114
                     /locus_tag="O0305_000248"
     CDS             2350..3114
                     /locus_tag="O0305_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0273434.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(3106..3357)
                     /locus_tag="O0305_000249"
     CDS             complement(3106..3357)
                     /locus_tag="O0305_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273435.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            3746..5011
                     /gene="femX"
                     /locus_tag="O0305_000250"
                     /gene_synonym="fmhB"
     CDS             3746..5011
                     /gene="femX"
                     /locus_tag="O0305_000250"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCY0273436.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMKTSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            5128..8295
                     /locus_tag="O0305_000251"
     CDS             5128..8295
                     /locus_tag="O0305_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCY0273437.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(8482..8799)
                     /gene="mspA"
                     /locus_tag="O0305_000252"
     CDS             complement(8482..8799)
                     /gene="mspA"
                     /locus_tag="O0305_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCY0273438.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(9044..9208)
                     /locus_tag="O0305_000253"
     CDS             complement(9044..9208)
                     /locus_tag="O0305_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273439.1"
                     /translation="MSLENQLAELKYDYVRLQGDIEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            9341..10249
                     /locus_tag="O0305_000254"
     CDS             9341..10249
                     /locus_tag="O0305_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCY0273440.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFVMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVYVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     TFIGMTTNLTIIISIIILYVYQAVFV"
     gene            10420..10551
                     /locus_tag="O0305_000255"
     CDS             10420..10551
                     /locus_tag="O0305_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273441.1"
                     /translation="MYGAYLIYEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(10668..11531)
                     /locus_tag="O0305_000256"
     CDS             complement(10668..11531)
                     /locus_tag="O0305_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCY0273442.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(11712..12599)
                     /locus_tag="O0305_000257"
     CDS             complement(11712..12599)
                     /locus_tag="O0305_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273443.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            12665..12760
                     /locus_tag="O0305_000258"
     CDS             12665..12760
                     /locus_tag="O0305_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273444.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            12786..14921
                     /locus_tag="O0305_000259"
     CDS             12786..14921
                     /locus_tag="O0305_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCY0273445.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYFTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            15034..16368
                     /locus_tag="O0305_000260"
     CDS             15034..16368
                     /locus_tag="O0305_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCY0273446.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(16560..16949)
                     /locus_tag="O0305_000261"
     CDS             complement(16560..16949)
                     /locus_tag="O0305_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273447.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            17306..17614
                     /gene="rpsJ"
                     /locus_tag="O0305_000262"
     CDS             17306..17614
                     /gene="rpsJ"
                     /locus_tag="O0305_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCY0273448.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            17642..18304
                     /gene="rplC"
                     /locus_tag="O0305_000263"
     CDS             17642..18304
                     /gene="rplC"
                     /locus_tag="O0305_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCY0273449.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            18331..18954
                     /gene="rplD"
                     /locus_tag="O0305_000264"
     CDS             18331..18954
                     /gene="rplD"
                     /locus_tag="O0305_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCY0273450.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            18954..19229
                     /gene="rplW"
                     /locus_tag="O0305_000265"
     CDS             18954..19229
                     /gene="rplW"
                     /locus_tag="O0305_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCY0273451.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            19262..20095
                     /gene="rplB"
                     /locus_tag="O0305_000266"
     CDS             19262..20095
                     /gene="rplB"
                     /locus_tag="O0305_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCY0273452.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            20162..20440
                     /gene="rpsS"
                     /locus_tag="O0305_000267"
     CDS             20162..20440
                     /gene="rpsS"
                     /locus_tag="O0305_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCY0273453.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            20469..20822
                     /gene="rplV"
                     /locus_tag="O0305_000268"
     CDS             20469..20822
                     /gene="rplV"
                     /locus_tag="O0305_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCY0273454.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            20846..21499
                     /gene="rpsC"
                     /locus_tag="O0305_000269"
     CDS             20846..21499
                     /gene="rpsC"
                     /locus_tag="O0305_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCY0273455.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            21502..21936
                     /gene="rplP"
                     /locus_tag="O0305_000270"
     CDS             21502..21936
                     /gene="rplP"
                     /locus_tag="O0305_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCY0273456.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            21926..22135
                     /gene="rpmC"
                     /locus_tag="O0305_000271"
     CDS             21926..22135
                     /gene="rpmC"
                     /locus_tag="O0305_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCY0273457.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            22159..22422
                     /gene="rpsQ"
                     /locus_tag="O0305_000272"
     CDS             22159..22422
                     /gene="rpsQ"
                     /locus_tag="O0305_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCY0273458.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            22454..22822
                     /gene="rplN"
                     /locus_tag="O0305_000273"
     CDS             22454..22822
                     /gene="rplN"
                     /locus_tag="O0305_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCY0273459.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            22858..23175
                     /gene="rplX"
                     /locus_tag="O0305_000274"
     CDS             22858..23175
                     /gene="rplX"
                     /locus_tag="O0305_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCY0273460.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            23202..23741
                     /gene="rplE"
                     /locus_tag="O0305_000275"
     CDS             23202..23741
                     /gene="rplE"
                     /locus_tag="O0305_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCY0273461.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            23764..23949
                     /locus_tag="O0305_000276"
     CDS             23764..23949
                     /locus_tag="O0305_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCY0273462.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            23981..24379
                     /gene="rpsH"
                     /locus_tag="O0305_000277"
     CDS             23981..24379
                     /gene="rpsH"
                     /locus_tag="O0305_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCY0273463.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            24404..24940
                     /gene="rplF"
                     /locus_tag="O0305_000278"
     CDS             24404..24940
                     /gene="rplF"
                     /locus_tag="O0305_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCY0273464.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            24971..25330
                     /gene="rplR"
                     /locus_tag="O0305_000279"
     CDS             24971..25330
                     /gene="rplR"
                     /locus_tag="O0305_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCY0273465.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            25351..25851
                     /gene="rpsE"
                     /locus_tag="O0305_000280"
     CDS             25351..25851
                     /gene="rpsE"
                     /locus_tag="O0305_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCY0273466.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            25868..26047
                     /gene="rpmD"
                     /locus_tag="O0305_000281"
     CDS             25868..26047
                     /gene="rpmD"
                     /locus_tag="O0305_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCY0273467.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            26064..26504
                     /gene="rplO"
                     /locus_tag="O0305_000282"
     CDS             26064..26504
                     /gene="rplO"
                     /locus_tag="O0305_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCY0273468.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            26504..27796
                     /gene="secY"
                     /locus_tag="O0305_000283"
     CDS             26504..27796
                     /gene="secY"
                     /locus_tag="O0305_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCY0273469.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            27813..28460
                     /locus_tag="O0305_000284"
     CDS             27813..28460
                     /locus_tag="O0305_000284"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCY0273470.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            28653..28871
                     /gene="infA"
                     /locus_tag="O0305_000285"
     CDS             28653..28871
                     /gene="infA"
                     /locus_tag="O0305_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCY0273471.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            28903..29016
                     /gene="rpmJ"
                     /locus_tag="O0305_000286"
     CDS             28903..29016
                     /gene="rpmJ"
                     /locus_tag="O0305_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCY0273472.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            29039..29404
                     /gene="rpsM"
                     /locus_tag="O0305_000287"
     CDS             29039..29404
                     /gene="rpsM"
                     /locus_tag="O0305_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCY0273473.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            29428..29817
                     /gene="rpsK"
                     /locus_tag="O0305_000288"
     CDS             29428..29817
                     /gene="rpsK"
                     /locus_tag="O0305_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCY0273474.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            29892..30836
                     /locus_tag="O0305_000289"
     CDS             29892..30836
                     /locus_tag="O0305_000289"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCY0273475.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            30853..31221
                     /gene="rplQ"
                     /locus_tag="O0305_000290"
     CDS             30853..31221
                     /gene="rplQ"
                     /locus_tag="O0305_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCY0273476.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            31759..32568
                     /locus_tag="O0305_000291"
     CDS             31759..32568
                     /locus_tag="O0305_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0273477.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPYDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTATEIFDNAEELTRIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            32565..33425
                     /locus_tag="O0305_000292"
     CDS             32565..33425
                     /locus_tag="O0305_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCY0273478.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKKKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            33415..>33519
                     /locus_tag="O0305_000293"
     CDS             33415..>33519
                     /locus_tag="O0305_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0273479.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIIL"
BASE COUNT        12177 a         5087 c         6361 g         9894 t
ORIGIN      
        1 aaatgctaag tttgatatat aataaattta agtaattgta taataatatg aattacaaac
       61 atctaagaag aaacatagga ggcatcatat tatgagtaat aaagttcaac gttttataga
      121 agcagaaagg gagttaagtc agttaaagca ctggttaaaa acaacacata agatttcaat
      181 tgaagaattt gtagtccttt ttaaagtgta tgaagctgaa aagattagcg gtaaagaatt
      241 gagggataca ttacattttg aaatgctatg ggatacaagt aaaatcgatg tgattatccg
      301 taaaatctat aaaaaagagc ttatttctaa attgcgttct gaaacggatg aaagacaagt
      361 attctatttc tatagtactt ctcaaaagaa attgttagat aaaattacta aagaaataga
      421 agtgttaagc gttacaaact aaaaacttaa aaagcatgcc aatcagtaca tcataattgc
      481 gtcttgggga cagacaaatg atgaatagag attggcatgc tttttatttt tgaatataaa
      541 tatttagttc atggcatttc tagttacatg acgtccatga attaagaagt aaacaagcat
      601 agtaatgatt gctaaagcgg ccataaagcc gaagatttca ctatatgaaa acatatgagt
      661 aaataaccca aggaatgatg gaccgaagcc gacacctgca tctagaccaa cgtaaaaagt
      721 agatgtcgcg ataccatatt taatcggggg tgagactttt atcgcaatag attgcattgc
      781 agatgataaa tttccatacc ctaaacctag gcaagcacca gcaagtaata ttaaccagct
      841 ttgatagctt gaaattaagc atacaaatga aaggaaaagc atgataaatg ctgggtagac
      901 aataatattt tcatttttat catccatcaa tctaccagca ataggtctag taattaacga
      961 tgctatagca tagcaaataa agaaatagct tgctgcagtg actaggtgtc gctctaaagc
     1021 aaatgcttgt aaataagtta ggatggacgc ataggtaacg ccaattaaaa gcataattac
     1081 agcaacagga atggcctctt ttgcaataaa ttgatgaata ctaaatcttg gtttatcaat
     1141 gacattagtt tcagttttgt tatttgttac ttcgaaatca acttttataa ataatgagat
     1201 aatgagtccg agtatgccta atatgacaca aataataaac agtaagtcaa ttgcgtattt
     1261 tgtaataagt aacatgccta gaaatgggcc aatcgctgta cctaatacta aacttaagga
     1321 aaataaactg atgccttcac tttttctatt aacaggggta acgtatgccg caatagtacc
     1381 tgttgcagtt gtcacaactg cagttgcgat accgtttatg agacgtacaa agattaaaaa
     1441 agctaaagat ccatcaataa aataaagtaa ttgcgtgata attaaagcaa ttaaaccaat
     1501 aaataataat cgtttaggtc cgatttgatt tacaaattta cctgtagcaa atcgaccaat
     1561 taaagacccg acaataaata gtcctacaac taatccggct aatgaatcgg aagcgtcgaa
     1621 agcttgctta ctatagccag caataactac aagtaacaaa tacatgcata ggtatacgat
     1681 aaagttgact aaaaagttaa ttgtaaaact tttggtgaaa ataggtgatt tatttaatga
     1741 gccttgatta gtcattgtgt gcctccctta ataatatttc atgaatttga ttcatcacat
     1801 ttatggtttc ttgcatttga tcttgcgaaa tgtttgtttt ggcaactact gcttgttgga
     1861 gcttttgtac atcaagacaa acaatctcat ataattcatg ccctttaggt gttaaagtta
     1921 aaaacttttg tcgtttatcc tctaaactgt tttctactgt tataaggtca ttttctatta
     1981 gagcttttaa agtttttctt gcagtaggct tttcaattgc acgtctatgt gaaatttcaa
     2041 tgagagtagt gggctgatgt ttagcgatat cgcgtaagat taaccattgg ccataatata
     2101 tattgtgttt ttctaaaatc ggctcggcta attttaaata ggggcgatat attgttataa
     2161 aactattgaa aaattcttgt gatagcatag tgacatctcc taagacaaaa tagttagctt
     2221 agctaacctt tttacaacaa tagtaattat aaaacgggag caattagaaa tcaatatata
     2281 attattaaga gcaaaaataa ttatactttg ttaaaataag cgtaattaca tgtaaatagg
     2341 gggatactaa tgatattgaa atttgatcac atcattcatt atatagatca gttagatcgg
     2401 tttagttttc caggagatgt tataaaatta cattcaggtg ggtatcatca taaatatgga
     2461 acattcaata aattaggtta tatcaatgaa aattatattg agctactaga tgtagaaaat
     2521 aatgaaaagt tgaaaaagat ggcaaaaacg atagaaggcg gagtcgcttt tgctactcaa
     2581 attgttcaag agaagtatga gcaaggcttt aaaaatattt gtttgcgtac aaatgatata
     2641 gaggcagtta aaaataaact acaaagtgag caggttgaag tagtagggcc gattcaaatg
     2701 gaaagagata cacataaaga tggtaaggta aagtggcaat tgctttatat aatgaatcag
     2761 gatgatgatg aaattaagcc accatttttt attcaatggg aagaaagtga ttccatgcgt
     2821 actaaaaaat tgcaaaaata ttttcaaaaa caattttcaa ttgaaactgt tattgtgaaa
     2881 agtaaaaacc gatcacaaac agtatcgaat tggttgaaat ggtttgatat ggacattgta
     2941 gaagagaatg accattacac agatttgatt ttaaaaaatg atgatattta ttttagaatt
     3001 gaagatggta aagtttcaaa atatcattcg gttatcataa aagacgcaca agcaacttca
     3061 ccatattcaa tttttatcag aggtgctatt tatcgctttg aaccattagt ataaatatac
     3121 gtaagtgcta tgagcgagaa tgcccatatg aataatgaca agcacaatgg aaagaatcgt
     3181 taatatatta tttaatcgtg atgacttaat taaaatgaaa aagattgata atataaatgt
     3241 gaaaaagata agtataaccc gtaaactaaa gtaattcacg gtgagaggtt gactcaatgt
     3301 cataatgatt gcaacgatgt tcataattat aaatagactt aaaataattg ttctcatatc
     3361 aaacacctca ttgttagatt attgacatta taacaggggt aattgtatat gaacattaat
     3421 gtggttgctt gaggaaaaat ttattcattg aagtcaagtt ggttcatttt agaaatgaat
     3481 atcgtgttag atgatgaaag tatattgaag tataggtaac tagttgaaaa gtattaattg
     3541 tacgataaca ttaaatttaa cacgaaacat agatataaaa tgattcacaa ttaaaatggg
     3601 taaatttgaa cttgctaaac tattaattgg agcatggaca tttcaaaaat aagagttcaa
     3661 atcttacaca agctctgaat cgacactata agatacaaac tgtataatta aaggtattgt
     3721 taaatagaag gagatatcat aaatcatgga aaagatgcat atcactaatc aggaacatga
     3781 cgcatttgtt aaatcccacc caaatggaga tttattacaa ttaacgaaat gggcagaaac
     3841 aaagaaatta actggatggt acgcgcgaag aatcgctgta ggtcgtgacg gtgaagttca
     3901 gggtgttgcg cagttacttt ttaaaaaagt acctaaatta ccttatacgc tatgttatat
     3961 ttcgcgtggt tttgttgttg attatagtaa taaagaagcg ttaaatgcat tgttagacag
     4021 tgcaaaagaa attgctaaag ctgagaaagc gtatgcaatt aaaatcgatc ctgatgttga
     4081 agttgataaa ggtacagatg ctttgcaaaa tttgaaagcg cttggtttta aacataaagg
     4141 atttaaagaa ggtttatcaa aagactacat ccaaccacgt atgactatga ttacaccaat
     4201 tgataaaaat gatgatgagt tattaaatag ttttgaacgc cgaaatcgtt caaaagtgcg
     4261 cttggcttta aagcgaggta cgacagtaga acgatctgat agagaaggtt taaaaacatt
     4321 tgctgagtta atgaaaatca ctggggaacg cgatggcttc ttaacgcgtg atattagtta
     4381 ctttgaaaat atttatgatg cgttgcatga agatggagat gctgaactat ttttagtaaa
     4441 gttggatcca aaagaaaata tagcgaaagt aaatcaagaa ttgaatgaac ttcatgccga
     4501 aattgctaaa tggcagcaga agatgaaaac atctgaaaag caagctaaaa aagcgcaaaa
     4561 tatgattaat gatgcgcaaa ataaaattgc taaaaatgaa gatttaaaac gagacctaga
     4621 agctttagaa aaggaacatc ctgaaggtat ttatctttct ggtgcactat taatgtttgc
     4681 tggctcaaaa tcatattact tatatggtgc gtcttctaat gaatttagag attttttacc
     4741 aaatcatcat atgcagtata cgatgatgaa gtatgcacgt gaacatggtg caacaactta
     4801 cgatttcggt ggtacagata atgatccaga taaagactca gaacattatg gattatgggc
     4861 atttaaaaaa gtgtggggaa catacttaag tgaaaagatt ggtgaatttg attatgtatt
     4921 gaatcagcca ttgtaccaat taattgagca agttaaaccg cgtttaacaa aagctaaaat
     4981 taaaatatct cgtaaattaa aacgaaaata gattaacgac tgaaatctga acgctcataa
     5041 gactgtcatt tgcgttcaga tttttttaca caatatagaa tggttgagta aaatattttt
     5101 gaatatagtg aaagaggggg aagtactgtg ataaaaaagc tattacaatt ttctttaggg
     5161 aataagtttg ctatcttttt aatggttgtt ttagttgtct tgggcggtgt atatgcgagt
     5221 gctaaattga aattagaatt actaccaaat gtacaaaatc cagttatttc agttacaaca
     5281 acaatgccgg gtgcaacgcc acaaagtacc caagatgaaa taagtagtaa aattgacaat
     5341 caagtaagat cattggcata tgtgaaaaat gttaaaacgc aatccataca aaatgcttca
     5401 attgtaacag ttgaatatga aaataataca gatatggata aagcagaaga acagcttaaa
     5461 aaagaaatcg ataaaattaa atttaaagat gaagttggtc aaccagaatt aagacgtaat
     5521 tcgatggatg cttttccggt tttagcatat tcattttcaa ataaagagaa tgacttgaaa
     5581 aaagtaacga aagtactgaa tgaacaatta ataccaaaat tgcaaacggt agatggtgtg
     5641 caaaatgcgc aattaaatgg gcagacgaac cgtgaaatca cccttaaatt taagcaaaat
     5701 gaacttgaaa aatatgggtt gactgctgat gatgtagaaa actatctaaa aacggcaaca
     5761 agaacaacgc cacttggatt gttccaattt ggtgataaag ataaatcaat tgttgttgat
     5821 ggtcaatatc aatctgttga tgcttttaaa aacataaata ttccattaac gttggcagga
     5881 ggacaagggc aatctcaatc ccaaagtgac aataaacaaa attcagccat gtcagacgtt
     5941 aatcaggcat caccacagca aaattcaaaa gcgtcagcac caaataatat aagtggtatg
     6001 ccgacagcga aactaggaga tttagctgat attacagttg gtgatgtgcg tacttctatt
     6061 tctaaaacga atggaaagga tgcggttaat ctacaaataa ctaaagctca agatgccaat
     6121 acagttcaag tagccaaaga tgtacaacgt aaaattgata cattcgttga tgaaaataaa
     6181 gattttaatg tcacaaaaac aatggatact gcaaagcctg ttgagaaatc actttatacg
     6241 atggttgaaa aagcatcatt aggtacaatc gtggcaatta tagttatttt gctgttttta
     6301 agaaacattc gtacgacggc aatttctatt atatcgattc cgttatcact tcttatggcg
     6361 cttattgctc tgaaattgag tgatgtttca ttgaatatac taacgttagg tgcattaaca
     6421 gtagcgattg gacgtgtgat agacgattcg attgtagttg ttgaaaatat ttatcgacgc
     6481 ttaacagatt cagaagaaca actaaaaggt gaaaatttaa ttatcagtgc gacaactgaa
     6541 gtatttaaac caataatgtc atcgacacta gttactatta tcgtcttctt accacttgtg
     6601 tttgtatcag gttcagtagg cgaaatgttt agaccttttg cattggctat tgcatttagt
     6661 ttattagcat cgttattagt gtcaattaca ctcgttccag cgttggcagc tacactattt
     6721 aaaaaaggcg ttaaacgtcg taataaacaa catcaagaag gattaggtgt tgttagtaca
     6781 acttataaaa aagtattaca ttggtcatta aatcataagt ggattgtaat tatattaagt
     6841 acattaattt tggttgcaac tattgtattt ggaggaccga gactaggcac tagctttatt
     6901 tcagcaggtg acgataaatt tttagctatt acttatacac cgaagcctgg tgaaacggag
     6961 caagcagtgt tgaatcatgc gaaagatgtt gaaaaatatt taaaacagaa aaagcatgta
     7021 aaaacaattc aatactcagt tggcggtagt agtccagtag atccaacggg tagtacaaat
     7081 agtatggcaa tcatggttga atatgataat gacacgccta attttgatgt agaagcggat
     7141 aaggttatta aacatgcaga tggctttaaa catcctggag agtggaaaaa tcaagattta
     7201 ggaacaggtg caggtaataa atctgtagag gttactgtaa aaggtccatc aatggatgcc
     7261 ataaaatcaa ctgtaaaaga tattgaacag aaaatgaaac aggttaaagg actagccaat
     7321 gtcaaatctg atttatcgca aacatatgat cagtatgaaa ttaaagtcga tcaaaataaa
     7381 gcggcagaaa atggtatttc tgcaagtcaa cttgcaatgc acttgaatga aaacttacca
     7441 gaaaaaacag ttacgactgt taaagaaaat ggtaaaactg ttgatgttaa agtcaaacaa
     7501 aataagcaaa cagactggtc agaagataag ttgaataata taactttgaa aaagccgact
     7561 ggtggtacga ttaaattggg agatatcgct acgttagtta aaacaacgac accaagtaaa
     7621 ttgacgcaag aacaaggaga ttatgcaacg acggtatctg ctaaagtaac aaataaagat
     7681 gtgggtggca caacacgaca agtgatgtct aaaataaata atttggacaa accgaataat
     7741 gtaaaggtta atatcggtgg tgcatcagat gatattaaca atgcaatgac acaattagcc
     7801 tttgcaatgt tagctgcaat cattatcgta tatttaatcc tagttattac atttaaaggt
     7861 ggcctagcac catttacaat tttattctct ttaccattta cagttatcgg tgtaattatt
     7921 gcactattaa tcacaggaga aacaatatca gtaccaagtt taattggtat gctaatgtta
     7981 attggaatcg tagtaacaaa tgccattgtg ttaatagacc gtgttattaa taatgagcaa
     8041 cagggcatgg agatgaaaga agcattaatc gaagcaggcg gtactagaat tagaccgata
     8101 ttaatgacgg cgattgcaac aattggtgca ttagttcctt tgttatttgg tcaagatagc
     8161 tcgattctta tttcgaaagg attagctgct acagttattg gtggtttaat ttcatcaact
     8221 ttattaacac ttgtagttgt accagtaata tatgaaattc tctttacttt aaaaaaacga
     8281 ttcactaaac gatagataat ataaaagcca taaaagcggt catgatattg actactttta
     8341 tggcttttta aaatttgaaa ttttagacct tatttcacgt gcgatttaat cgatgtgtta
     8401 ataaaacaat actggcattc aacatgtaat ccgataataa aataaggttg caattatgtt
     8461 attgcatatt ttgtttatta cttagaataa tacgatggtt aagatgaaat atatgacaaa
     8521 gataaataat gacattaaat tcaagatgtt tacgcctttt agatcttttt taagcatttt
     8581 aaatcctgtc acttcgacat taccgactaa gagtaataag acgataaata cccaccaatt
     8641 ctcttttgga aaactcatcg tcgttaatgc taagacgaat aataacaaca cacccataat
     8701 aattctcaaa atgcgagtaa atacaacttc cattttaaaa atactaataa aactaacaat
     8761 tagataaagt attgctagta aaatcagata aaattgcata tgataacttc ctctccgttt
     8821 caaagggttc atgtagtaca gcagcgtaga aaacataaat agcagtgttg ggatagatat
     8881 gcgtgtatgt tgttaatgaa acactaaatc ataacacgac aacatcttgt gattctagat
     8941 gtaattgagc gaaaaatgaa gcaattcatc aatataacgt attcagagca atgctagtca
     9001 aatttatgta acaaaaagca tttattgctt gaatgataga ttattaatct tgcatttgag
     9061 cacgaacgtt tctaatttca ttttcaatat ctttaagttg acgaacaagt gcggaagtat
     9121 ctaaattcaa agattcccgt ttttctatgt caccttgaag acgaacataa tcatatttaa
     9181 gttcggctag ttggttttct aaagacatac tataacactc ctttgaaata taattaagtg
     9241 catttaatgt agattatagc atgaaacagt agacttctaa aatatctgtg gagtatgtta
     9301 aagttataaa ttatgaagat tattgaaaga agggataaca gtgacagaac aatttactat
     9361 gattatttta ttaattgcgc ttggttactt tttgaagcgc attaatttta taaaagcaac
     9421 agatagtcaa gtcattgcta cattagttct taatgtcaca ctcccatctt tagttattgt
     9481 gaatttaaac agtgcagaat taaaattgtc attctccatt ttaccaattt taatgattat
     9541 atatggtatt gtggcgaaaa tgattgctgt ctggtttttt agaaaatatg ataatcacat
     9601 gcgtggatca gtaggtatga tgactggtgc tatgaatatt ggattgtttg catatccact
     9661 aattgaagtg atatggccta aaacgggact tatttatttt gttatggctg atataggtgg
     9721 tgcctttgtg atgtttggaa ttacttactt tgtaggtagc tactttagtg aaggtagtga
     9781 tcaattcgat tttaaatatt taggtaagaa attattacaa tcagtgccac tcgttacata
     9841 cattgtaatg tttatattga atataagtca catccattta ccacatgtgg caatcgattt
     9901 ctttagcata ctttcccatg caaatatgcc gttatcaatg attttgctag gtgtcatgtt
     9961 gaattttact ctggaaagaa agtatatacc tgctactatt aaatatttat gtttgcatta
    10021 tggattagca ttagttgcgg gattgttagt atatgtgttg ttaccagtat cagatgatat
    10081 gattaagacg acattgttgg tcacttggat gttcccggta ggtgttgcaa ttatttctta
    10141 tggtattcag tttaaatatc gaacactgac atttataggt atgacgacca atttgacaat
    10201 cattattagt atcattattt tgtacgtgta tcaggcagtg tttgtctagt ttacacattt
    10261 gaaaataaaa tccattttag tattcgtaga aaatgagagc cccctttacc acaatatgta
    10321 aatgtatatt gtgaaaaggg ggcttttatt tatgacatag ttgttcaact catacattca
    10381 agttgataat gcagtgcgtt atgcagctat ccacttcata tgtatggcgc atacttgata
    10441 tatgaatatc atcaagattt taaaagtatc gctaatttta aagtcttaaa acgcatgatt
    10501 aatgtcatga tgtgttattg cgttttgttt ttagagccac gtatttccta ggacatatag
    10561 aggaacattc aacgtaaaat tcccgaaaat taattttaat cattctataa gtgaatggtt
    10621 attttcagaa gtgattacat ttttacgtta catgagtatt taactttcta tttcaaatta
    10681 cctagaatta tagcagctat cacgataata ataatacctg cccaaatacc cgtcatctga
    10741 cgacgatctt ttctttctcc taaaatgaaa ataccgccta aggttgaaac gataacaagt
    10801 aactgtgata atgagaaact tgtagctaca ccaacttttg gttgagaata gaacatgaac
    10861 aagttaccaa ttccccaaat cacacctggc aatagattaa gtgctgttga tttaagtgat
    10921 gttttatgat tcatggatag gataaagcca ccaattgcca taccgacaga ttggaagaac
    10981 aatgcatcag ttccaccaac accaaagatg tcaccaagta caacgaaacc tacatatcca
    11041 actgtagata caattaaaat acccattgct tttttaaatt caggattatc tgattgacgt
    11101 tcatttttag ctttaagtga agtaagtgct acaccagtaa ctaataatat catggcgatt
    11161 aaaccaaaga taatttgagt cattgaagac cattcaccta aaaagataac gctgaataat
    11221 gttgtaccaa ctaattgcat acctgtagaa actggcatag tatttgatac accgattaaa
    11281 ctaatagatt taagctgatt cgcttgtcca aacgcccata atgcaccaga aataagaccg
    11341 acaataatta cagtaggatt attgaatttt gcgaaaccag taataagtaa acctaatcca
    11401 acaatgagtg cgcctagcgt cgtaccacga atttggttgt aaggtccacc gccgacgaac
    11461 acattaataa gaacgacact tccccagaat aaagcaggta aaagtgcgat taagaaatca
    11521 agaaattgca agactactca ctctcctata ctatttatgc aagtgctccc cttaattgac
    11581 acttgcatgt tattgtaaag cattctgccc taatagtaaa aaacaactac ttataatttt
    11641 caatatttac aaaaagtaga aaaacccaca atacatgcta atgatgtact gtagggtatt
    11701 tgcttggtgt tttattcagg gattgtttga tcttttacgt tacgtgattc gaatttgaat
    11761 ttaatgtact catagttgat atggaaacct agaccattaa atcgttcgat gagtttatta
    11821 tttttattac gaattttaaa ataaactttt ctaatatcaa aattatcaaa agcatattgc
    11881 aatgcatacg caagcaactc gaaagcgata cctttcaaac ggtaatctac atgagaactg
    11941 aagtatttaa tctcggcgat tgactgttgt gaatcaattt caaggtataa ataaccttta
    12001 agtaaacctt cgctaacaaa taaaaacaaa tgatgatggt cgtctaaact atcaatgata
    12061 tcttgtggtg actgtgcctg atatttaaat gtagttgtgt gtaatttgct gaaagcacga
    12121 taaaaccctt tgtgataagg aatgatattt ggttggtgca tatcttcttc taatcttgta
    12181 cgggcttcta tgtaatagtc agtgaagtta taacttgctt gaataacttt cattaatggt
    12241 ttgtattgtt gaatgccttc ttcaaaagaa aagttaaaga cggcatcgtc aggttggttc
    12301 gaagtcattg ctttaaatag cgtttcaaac gtatcgacag ataatacata attgtcagcc
    12361 acgaaagggc ctatgacttt gaatttattt ttatcgtatg caaaagacaa tatgattgct
    12421 ttgattgaat cgttttcttt aatcgaaaat acaccaggtg attcaatcgc atcggttatt
    12481 aatacatcta tttcattgta ctgttgagga agtttatata agtatgatgc tgattcataa
    12541 ttcgaatgtt caatgaacga ttttatttgt gtaatatcat ataactgaac tatgctcatg
    12601 tcattacctc cgtacttttt gttactttta ttatatagta tttcaactga aatgaaagtt
    12661 aatagtgata ttaacatgtt acaatacatt taacacatca tttgcaatca ttgaatttaa
    12721 atcaaagatt agtggaatag atgaagcacg ttcgaaataa aagaacgtat gagaaaggat
    12781 aatttatgaa atctttaata ttagctgaaa aaccatcagt cgcaagagat attgctgatg
    12841 ctttacaaat aaatcagaag cgtaatggtt actttgaaaa taaccaatat attgtcacgt
    12901 gggcgttagg tcatctagtg acaaatgcga cacctgaaca atacgataaa aatttaaagg
    12961 aatggcgatt agaagacctt ccaattatac ctaaatatat gaaaactgtt gttattggta
    13021 aaacaagcaa acaatttaaa acagtaaaag cgttaatttt agataataaa gtgaaagata
    13081 ttattattgc aacagatgct ggacgagaag gtgaactagt tgcaagattg attttggata
    13141 aagttggtaa caaaaagcca atccgtcgat tatggattag ctcagttact aaaaaagcta
    13201 ttcaacaagg ttttaaaaat ttaaaagacg gtcgtcaata taacgatttg tattatgcag
    13261 cgttagcgag aagtgaggca gattggattg ttgggattaa tgcaacgcgt gcactaacaa
    13321 caaagtatga tgcacagcta tccctgggac gtgttcagac accaacgatt caattagtaa
    13381 atacacgaca acaagagatt aatcagttca aaccacaaca atactttaca ttatcattaa
    13441 cggtaaaagg gtttgatttt cagctagaat caaatcagcg atataccaat aaagaaactt
    13501 tagaacagat ggttaataat ttgaaaaatg tcgatggtaa gattaaatct gttgctacta
    13561 aacataagaa gtcgtatccg caatcactgt acaatttaac agatttacaa caagatatgt
    13621 atagacgtta taaaattgga cctaaagaaa cattgaatac acttcaaagc ttatatgaga
    13681 gacataaagt cgtaacctat ccaagaacag attcaaacta tttaacaact gatatggtag
    13741 atactatgaa agaacgtatt caggcgacga tggcaacaac atataaagac caagcacgcc
    13801 cattaatgtc taaaacattt tcatcaaaaa tgtcgatatt taataatcaa aaagtatctg
    13861 atcaccatgc aattattcct acagaagtga gacctgtcat gtcagactta agtaatagag
    13921 aattaaagtt atacgatatg attgtcgagc gttttttaga agctttaatg cctccgcacg
    13981 agtatgacgc gataactgta actttagagg ttgcagggca cacatttgtt ttgaaagaga
    14041 atgtaacaac tgttttaggt tttaaatcta ttagacaagg tgaatctatt acagagatgc
    14101 aacagccttt ttcagaaggc gatgaagtga agatttcaaa aacaaatatt agagaacatg
    14161 aaacaacacc tccagaatat tttaatgaag gttcgttatt aaaagcgatg gagaaccctc
    14221 agaactttat tcaattgaag gataaaaaat atgcgcaaac tttaaaacaa acaggtggta
    14281 tcggcacagt tgcaacaagg gccgacatta tcgataaatt atttaatatg aatgccattg
    14341 aatcaagaga cggtaaaatt aaagtaacgt caaaaggtaa acaaatatta gaattagcac
    14401 cagaagaatt aacgtcgcca cttttaactg cacaatggga agaaaaatta cttttaattg
    14461 aacgtggtaa atatcaggcg aaaacattta ttaatgaaat gaaagatttt acgaaagatg
    14521 ttgtaaatgg gattaaaaat agtgatcgta aatataaaca cgataattta acaaccacag
    14581 aatgcccaac gtgtggtaaa ttcatgatta aagttaaaac taaaaatggt cagatgcttg
    14641 tgtgccaaga tccatcttgt aagacgaaaa agaatgtaca gcgcaaaaca aatgcaagat
    14701 gtccaaactg taaaaagaaa ttaacgttgt ttggtaaagg gaaagaagcg gtatatcgtt
    14761 gtgtttgtgg acattctgaa acgcaagcac atatggatca gcgtatgaag tctaaatcct
    14821 ctggtaaagt atctcgtaaa gaaatgaaaa agtatatgaa taaaaatgaa ggtttagaca
    14881 ataatccgtt taaagatgca ttaaagaact tgaatttata gataaaatcg aacaaagttg
    14941 aatcagaaaa acgaaaagtt cgcttttggt attgtttttt attaagaatg atattaaact
    15001 attaaggtat tttaaaaaaa ggagcatcca ttcgtgaaaa actatttcca gttcgataaa
    15061 tatggaacaa actttaaaag agaaatctta ggcggtatca caactttctt atctatggcc
    15121 tatattttag cagttaaccc gcaagtttta agtttagcag gtgttaaagg cgtatcagaa
    15181 gatatgaaaa tggaccaagg tgccattttt gtagcgactg cattagcagc atttgtaggc
    15241 tcgctattca tggggctaat agctaaatat ccaattgcgt tagcaccagg tatgggatta
    15301 aatgcattct ttgcatttac tgttgtgtta accatgggta ttccttggca agttggttta
    15361 acaggcgttt tattctcagg tatattcttt gcgatattaa ccgtcacagg gtttagggaa
    15421 gttattatta atgcaattcc ttatcaaatg aagatggctg tttcagcagg tataggatta
    15481 ttcattacat ttgtaggttt acaaagttcg ggtattatag ttaaaaatga atctacatta
    15541 gtaacattgg gacatttaac tgatccacca gtacttttgg cgatattcgg tatcgttatt
    15601 acagttattt tatatgcaat taaattgccg ggttctatct ttatcggtat gatcatcaca
    15661 gcaatcgttg gtatgttcac tggattgatt caaatgccat ctggtattgt gggtaagatt
    15721 ccaagtattg aaccaacgtt cggtgctgca tttgaagcgt ttaaagaccc aagtcaatta
    15781 ttaacgatac agttcttaat tgttatttta acgttcttat ttattgattt ctttgataca
    15841 gctggtacat tagtagctgt tgctactcaa gcaggtatta tgaaagataa taaattacca
    15901 agagctggta gagcattgtt ctcagattct ttagctacga ttgtaggatc agtttttgga
    15961 acaactacaa caacttctta tatcgaatca acttcaggtg ttgctgtagg tgctagaaca
    16021 ggatttgcta gtatcgtaac aggttgttgt ttcctattag cgctgttctt tagtccgtta
    16081 atcgctgttg taacaagtgc agttacgaca ccagccttag tggtggtagg tgtattaatg
    16141 gctgcaaact ttgctgagat aaactggaaa agttttgaag ttgcagtgcc agccttcatt
    16201 acaatcatta tgatgccttt atcatattca attgcaactg gtattgcgtg tggatttatt
    16261 ttctatccaa ttacaatgct tatctctaaa aaacataaag aagtacatcc gataatgtat
    16321 gtattaatgg tactattcat tctgtacttc atatttgttc atggttaaat ccagacacaa
    16381 aaaacgactg ccaatgattg gtagtcgttt ttttctttga agtaaggata aaataagaag
    16441 cgaaataaca taaagcgata ttatgaatgt ctttaaatat ccgatataaa tatacttctt
    16501 ttaaaattgt tggacgtgtt tcttctatat tatataccta attaaaaagg ttttaagaat
    16561 tattctctat gtccatcttt gtattgtgtg taaatgacac gagcattttt attaggtata
    16621 taggttggcg ctgataatac aactaggaca aatagaatca ttaggaaaaa taaaatccaa
    16681 tgaatcaaaa ttcctaagaa ggggataata gctaaaaaac tacctactat accaaatatt
    16741 ggaataatcg ctataggtct aatcgcattt tgtctattca ctaataatat aatagtaaga
    16801 attagataaa gtaatgcatt aaagagtaaa ggctgccatc caaaacttaa aataaacgtt
    16861 gcacctaaaa aaggaatgcc atagaaaaac tcacaaagta ttaaaaagat acctataagt
    16921 gctaaagata attttggtcc atttttcata ttcattcacc accgttctta tgactaatta
    16981 tatagaataa ttttcaatga taataataaa atgattcaag gtacgaattt acaataaaga
    17041 aggaaataaa aaatatctcg aaaatagttg aactgactaa gtatataaag taaaatataa
    17101 aagtatgtgt tagacagata gaaaaattat gtgaaaagcc ttgatttatc gatgtttttc
    17161 gtgtaatata actaacgttg gactttaaga aaagcgatga agcgaaaggt tactgacaca
    17221 cccggccgct ttgccatggc gctgtgtaag atagttttcg tggagaagtc tatcactaaa
    17281 tgtagacgaa taaggaggga aaattatggc aaaacaaaaa atcagaatca gattaaaagc
    17341 ttatgatcac cgcgtaattg atcaatcagc agagaagatt gtagaaacag cgaaacgttc
    17401 tggtgcagat gtttctggac caattccgtt accaactgag aaatcagttt acacaatcat
    17461 ccgtgccgtg cataagtata aagattcacg tgaacaattc gaacaacgta cacacaaacg
    17521 tttaatcgat attgtaaacc caacaccaaa aacagttgac gctttaatgg gcttaaactt
    17581 accatctggt gtagacatcg aaatcaaatt ataatagaca attttaggag gtggactttc
    17641 gatgaccaaa ggaatcttag gaagaaaaat tgggatgaca caagtattcg gagaaaacgg
    17701 tgaattaatc cctgtaacag tagtagaagc taaagaaaat gttgtattac aaaagaaaac
    17761 tgtagaagtt gatggataca acgcaatcca agttggattt gaagacaaaa aagcatacaa
    17821 aaaagatgca aaatctaata aatatgctaa taaaccagct gaaggtcacg ctaaaaaagc
    17881 tgacgcagca cctaagcgct tcattcgtga attccgcaat gtagacgtgg atgcttacga
    17941 agtaggtcaa gaagtctcag tagatacttt tgtagctggc gacgttattg acgtaacagg
    18001 cgtatcaaaa ggtaaaggtt tccaaggtgc aattaaacgc cacggacaat ctcgtggacc
    18061 tatgtcacac ggttctcatt tccacagagc accaggttct gtaggtatgg cttcagatgc
    18121 ttctagagta tttaaaggcc aaaaaatgcc aggacgtatg ggtggaaaca ctgtaactgt
    18181 tcaaaactta gaagtagttc aagttgacac agaaaacaaa gttatcttag taaaaggtaa
    18241 cgtacctgga cctaaaaaag gtttagtaga aatcagaact tcaattaaaa aaggtaataa
    18301 ataataagaa gtgaaaggag gaaatgcata atggctaatt atgatgtttt aaaattagac
    18361 ggaactaaat caggttcaat cgaattaagc gatgcagtat tcggtattga gccaaataat
    18421 agcgttttat tcgaagctat taatttacaa cgtgcttcat tacgtcaagg tacgcatgct
    18481 gttaagaatc gttcagcagt aagcggtggc ggacgtaaac catggaagca aaaaggaaca
    18541 ggtcgtgctc gtcaaggtac aatccgtgct ccacaatggc gtggcggtgg tatcgtattc
    18601 ggaccaactc caagaagtta tgcatacaaa atgcctaaga aaatgcgtcg tttagcttta
    18661 cgctcagcat tatctttcaa agctcaagag aatggcttaa ctgtagttga cgcattcaac
    18721 ttcgaagctc caaaaactaa agaattcaaa aatgtattat ctacattaga acaacctaaa
    18781 aaagtattag tagttactga aaacgaagat gtaaatgttg aattatcagc acgcaacatc
    18841 cctggcgttc aagtgacaac tgctcaaggt ttaaatgttt tagatatcac taatgctgac
    18901 agcttagtaa ttactgaagc tgctgctaaa aaagttgagg aggtgctcgg ataatggaag
    18961 caagagatat tcttaagcgc cccgtaatca ctgagaaatc ttctgaagca atggctgaag
    19021 acaaatacac tttcgacgtt gatactcgtg ttaacaaaac acaagtaaaa atggcagttg
    19081 aagaaatctt caacgtaaaa gttgcaagtg ttaatatcat gaattacaaa cctaagaaaa
    19141 aacgtatggg ccgttaccaa ggctatacaa acaaaagaag aaaagcgatt gtaactctta
    19201 aagaaggatc aatcgactta tttaactaat aaaacaatta ccattaagga ggtaagcgac
    19261 aatggctatt aaaaagtata agccaataac aaatggtcgt cgtaatatga cttcgttaga
    19321 tttcgcagaa atcacgaaaa ctacacctga aaagtcatta ttaaaaccgc taccgaaaaa
    19381 agcgggacgt aacaaccaag gtaaattgac tgtaagacac catggtggtg gacacaaacg
    19441 tcaataccgt gttatcgatt tcaaacgtaa caaagatggt atcaatgcaa aagttgattc
    19501 tattcaatat gatccaaacc gctcagcaaa catcgcttta gttgtatatg cagacggtga
    19561 aaaacgatat atcattgctc ctaaaggatt agaagtaggt caaatcgttg aaagtggtgc
    19621 tgaagctgac atcaaagttg gtaacgcatt accattacaa aacattccag ttggtacagt
    19681 agtacacaac atcgagctta aacctggtaa aggtggacaa atcgctcgtt cagctggtgc
    19741 aagtgctcaa gtacttggta aagaaggtaa atacgtatta atcagattaa gatctggtga
    19801 agttcgtatg atcttatcta cttgccgtgc tacaatcggt caagttggta acctacaaca
    19861 cgaattagtt aacgttggta aagccggacg ttcaagatgg aaaggtatcc gtccaacagt
    19921 tcgtggttct gtaatgaacc ctaacgatca cccacacggt ggtggtgaag gtcgtgctcc
    19981 tatcggtaga ccatctccaa tgtcaccatg gggtaaacct acgcttggta agaaaactcg
    20041 tcgtggtaaa aaatcatcag acaaacttat cgttcgtgga cgtaagaaaa aataatataa
    20101 tcaacttatt tgggtgtgcg gcttaaagct gcacgcacat aataagaagg gaggcgccca
    20161 aatggctcgt agtattaaaa aaggaccttt cgtcgatgag catttaatga aaaaagttga
    20221 agctcaagaa ggaagcgaaa agaaacaagt aatcaaaaca tggtcacgtc gttctacaat
    20281 tttccctaat ttcatcggac atacttttgc agtatacgac ggacgtaaac acgtacctgt
    20341 atatgtaact gaagatatgg taggtcataa attaggtgag tttgctccta ctcgtacatt
    20401 caaaggacac gttgcagacg acaagaaaac aagaagataa tatctattaa gtagaggagg
    20461 acatcctaat ggaagcaaaa gcggttgcta gaacaataag aatcgcacct cgtaaagtaa
    20521 gactagttct tgacttaatc agaggtaaaa atgctgctga agctattgca attttaaaat
    20581 taacaaacaa agcttcatca ccagtaattg aaaaagtatt aatgtccgct ttagctaatg
    20641 ctgaacataa ctatgacatg aacacagatg aattagtagt taaagaagca tatgctaacg
    20701 aaggaccaac attaaaacgt ttccgtccac gtgcgcaagg tcgtgcaagt gcgattaaca
    20761 aacgtacaag ccacattaca atcgtcgtaa gtgacggtaa agaagaagct aaagaagctt
    20821 aattaacttt taaggaggga atactgtggg tcaaaaaatt aatccaatcg gacttcgtgt
    20881 tggtattatc cgtgattggg aagctaaatg gtatgctgaa aaagacttcg cttcactttt
    20941 acacgaagat ttaaaaatcc gtaaatttat tgataatgaa ttaaaagaag catcagtttc
    21001 tcacgtagag attgaacgtg ctgcaaaccg tatcaacatt gcaattcata ctggtaaacc
    21061 tggtatggta attggtaaag gcggttcaga aatcgaaaaa ttacgcaaca aattaaatgc
    21121 gttaactgat aaaaaagtac acatcaacgt aattgaaatc aaaaaagttg atcttgacgc
    21181 tcgtttagta gctgaaaaca tcgcacgtca attagaaaac cgtgcttcat tccgtcgtgt
    21241 acaaaaacaa gcaatcacta gagctatgaa acttggtgct aaaggtatca aaactcaagt
    21301 atctggtcgt ttaggcggag ctgacatcgc tcgtgctgaa caatattcag aaggaactgt
    21361 tccacttcat acgttacgtg ctgacatcga ttatgcacac gctgaagctg acactactta
    21421 cggtaaatta ggcgttaaag tatggattta tcgtggagaa gttcttccta ctaagaacac
    21481 tagtggagga ggaaaataat aatgttacta ccaaaacgtg taaaatatcg tcgtcaacat
    21541 cgtcctaaaa caactggtcg ttctaaaggc ggtaactacg taacatttgg tgagtttggt
    21601 ttacaagcta caacaacgtc ttggatcaca tctcgtcaaa tcgaatctgc tcgtatagca
    21661 atgacacgtt acatgaaacg tggcgggaaa gtttggatta aaatcttccc acatacacca
    21721 tatactaaaa aacctttaga agtacgtatg ggtgctggta aaggtgcggt tgaaggctgg
    21781 atcgcagttg ttaaaccagg tagaatttta ttcgaagttg ctggcgtttc tgaagaagtt
    21841 gcgcgtgaag cactacgttt agcaagtcac aaacttccag taaaaactaa gtttgtaaaa
    21901 cgtgaggaat tgggtggtga aacaaatgaa agctaaggaa attagagact taaccacttc
    21961 agaaatcgaa gaacaaatca aatcttcaaa agaagagctt tttaacctac gctttcagtt
    22021 agctacaggt caattagaag aaactgcacg tattcgtaca gtaagaaaaa cgattgcacg
    22081 tctaaaaact gttgctcgtg aaagagaaat tgaacaaagt aaggctaatc aataattaag
    22141 taagaggagg ttacaaaagt gagcgaaaga aacgatcgta aagtttatgt aggtaaagtt
    22201 gtttcagaca aaatggacaa gactattaca gtacttgttg aaacttacaa aacacacaaa
    22261 ttatacggta aacgagtaaa atactctaaa aaatacaaaa ctcatgatga aaacaattca
    22321 gctaaattag gagacattgt taaaattcaa gaaactcgtc ctttatcagc aacaaaacgt
    22381 tttcgtttag tagagattgt tgaagagtca gtaattattt aatacaagtt tagagataag
    22441 ggaggtttaa ctaatgatcc aacaagaaac acgcttgaaa gtagcagaca actctggtgc
    22501 tcgtgaagtt cttacaatca aagtattagg tggatctggt cgtaaaacag caaacatcgg
    22561 cgatgttatc gtatgtactg ttaaaaatgc aacaccaggt ggcgttgtta aaaaaggtga
    22621 cgttgtcaaa gctgtaatcg tacgtactaa gtcaggtgtt cgtcgtaatg acggttcata
    22681 catcaaattt gatgaaaatg catgtgttat catccgtgat gacaaaggcc cacgtggtac
    22741 tcgtatcttc ggacctgttg ctcgtgaatt acgtgaaggt aacttcatga aaatcgtatc
    22801 attagcacca gaagtacttt aattaataac aaccaataca aataaggagg tgcccacatg
    22861 catatcaaaa aaggtgacaa cgttaaagtt atcgcaggta aagacaaagg taaagaaggt
    22921 aaagtaattg ctactctacc taaaaaagac cgtgtcgttg tggaaggtgt taacattatg
    22981 aaaaaacacc aaaaaccaac tcaattaaat cctgaaggtg gaatcttaga aacagaggca
    23041 gcaatccatg tttctaatgt acaattattg gaccctaaaa caaacgaacc aactcgtgta
    23101 ggttacaaat ttgttgatgg taaaaaagtt cgtatcgcta aaaaatctgg cgaagaaatt
    23161 aaatctaata attaataaca agttgaaagg aggatcccac tttgaaccgt ttaaaagaaa
    23221 agtttaacac tgaagttact gaaaacttaa tgaaaaaatt caattatagt tcagtaatgg
    23281 aagtaccaaa aatagataaa atcgttgtga acatgggtgt aggtgacgca gtacaaaatt
    23341 ctaaagtatt agacaatgct gttgaagaat tagaattgat cactggtcaa aaaccattag
    23401 taactaaagc taaaaaatca atcgcgactt tccgtttacg tgaaggtatg ccaatcggtg
    23461 cgaaagtaac acttcgcggt gaaagaatgt atgaattctt agacaaatta atttcagtat
    23521 cattaccacg tgtacgtgac ttccaaggtg tttctaaaaa agcatttgac ggacgcggta
    23581 actacacttt aggtgttaaa gaacaattaa ttttcccaga aatcgactat gataaagtaa
    23641 gtaaagttag aggaatggat attgttatcg taacgactgc taacactgat gaagaagctc
    23701 gtgaattgtt agctaacttc ggtatgccat tccgtaaata attatttaaa ggaggctaat
    23761 taagtggcta aaacttcaat ggttgctaag caacaaaaaa aacaaaaata tgcagttcgt
    23821 gaatacactc gttgtgaacg ttgtggccgt ccacattctg tatatcgtaa atttaaatta
    23881 tgccgtattt gtttccgtga attagcttac aaaggccaaa tccctggcgt tcgtaaagct
    23941 agctggtaat aaaaaagagt ctgaaaggag gcaacaatca atgacaatga cagatccaat
    24001 cgcagatatg cttactcgtg taagaaacgc aaacatggtg cgtcacgaga agttagaatt
    24061 acctgcatca aatattaaaa aagaaattgc tgaaatctta aagagtgaag gtttcattaa
    24121 aaatgttgaa tacgtagaag atgataaaca aggtgtactt cgtttattct taaaatatgg
    24181 tcaaaacgat gagcgtgtta tcacaggatt aaaacgtatt tcaaaaccag gtttacgtgt
    24241 ttatgcaaaa gctagcgaaa tgcctaaagt attaaatggt ttaggtattg cattagtatc
    24301 aacttctgaa ggtgtaatca ctgacaaaga agcaagaaaa cgtaatgttg gtggagaaat
    24361 tatcgcatac gtttggtaat aaaaaataag gaggtgccat aacatgagtc gtgttggtaa
    24421 gaaaattatt gacatcccta gtgacgtaac agtaactttt gatggaaatc atgtaactgt
    24481 taaaggtcct aaaggtgaat tatcaagaac tttaaatgaa agaatgacat tcaaacaaga
    24541 agaaaacaca attgaagttg taagaccatc tgattctaaa gaagatagaa caaaccatgg
    24601 tacaactcgt gctttattaa acaatatggt acaaggtgtt tctcaaggat acgtaaaagt
    24661 acttgaactt gttggtgtag gttaccgtgc tcaaatgcaa ggtaaagact taatccttaa
    24721 cgttggttat tctcacccag tagaaattaa agctgaagaa aacattactt tctcagttga
    24781 gaaaaacaca gtcgttaaag ttgaaggtat ttcaaaagaa caagttggag cattagcatc
    24841 taacatccgt tcagtaagac ctccagagcc ttacaaaggt aaaggtattc gttaccaagg
    24901 tgaatacgtt cgccgtaaag aaggtaaaac tggtaaataa tagataaact ctaaagaaag
    24961 gagtattgaa atgatcagta aaattgataa aaataaagtg cgtttaaaaa gacatgctcg
    25021 tgttcgtact aacttatcag gtacagctga aaagccacgt ttaaacgtat atcgttcaaa
    25081 caagcatatc tacgctcaaa ttattgatga taataaaggc gtaacattag ctcaagcttc
    25141 ttcaaaagac agcgacattg ctactacagc aactaaagtt gaattagcaa ctaaagtcgg
    25201 tgaagcaatt gctaaaaaag ctgctgacaa aggcattaaa gaaatcgtat ttgaccgtgg
    25261 aggatattta tatcacggac gtgttaaagc attagctgaa gcagcaagag aaagcggatt
    25321 agaattttaa tttaaaggag ggacaaatac atggctcgta gagaagaaga gacgaaagaa
    25381 tttgaagaac gcgttgttac aatcaaccgt gtagcaaaag ttgtaaaagg tggtcgtcgt
    25441 ttccgtttca ctgcattagt tgtagttgga gacaaaaatg gtcgtgtagg tttcggtact
    25501 ggtaaagctc aagaggtacc agaagcaatc aaaaaagctg ttgaagcagc taaaaaagat
    25561 ttagtagttg ttccacgtgt tgaaggtaca actccacaca caattactgg ccgttacggt
    25621 tcaggaagcg tatttatgaa accggctgca cctggtacag gagttatcgc tggtggtcct
    25681 gttcgtgccg tacttgaatt agcaggtatc actgatatct taagtaaatc attaggatca
    25741 aacacaccaa tcaacatggt tcgtgctaca atcgatggtt tacaaaacct taaaaatgct
    25801 gaagatgttg cgaaattacg tggcaaaaca gtagaagaat tatacaatta aggagggaaa
    25861 actagttatg gctaaattac aaattaccct cactcgtagt gttattggtc gtcctgaaac
    25921 acaacgtaaa actgttgaag ctttaggtct taaaaagact aacagttcag tagttgttga
    25981 agataaccct gctattcgtg ggcaaatcaa caaagttaag cacttagtaa cagtagaaga
    26041 aaaataatca aggaggtgcc gaaatgaaat tacatgagtt aaaaccggca gaaggttcac
    26101 gtaaagaacg caatcgtgtt ggacgtggtg ttgcgacagg taacggtaaa acaagtggtc
    26161 gcggacacaa aggtcaaaaa gctcgttcag gcggtggtgt aagaccagga tttgaaggtg
    26221 gtcaattacc attattccgt cgtttaccaa aacgtggttt tactaacata aatcgtaaag
    26281 aatatgctat tgttaactta gaccaactta ataaatttga agatggtact gaagtaactc
    26341 cagctttatt agtagaatct ggtgttgtta agaatgaaaa atctggtatc aaaatactag
    26401 gtaatggttc acttgataag aaattgacag tgaaagctca taaattctca gcttcagcag
    26461 cagaagctat tgatgctaaa ggtggagcac acgaggtgat ctaatgattc aaacccttgt
    26521 gaacttcttt agaacaaaag aggttcgtaa caagattttc ttcacactag caatgttagt
    26581 aatttttaaa atagggactt atataccagc tccaggagta aatcctgcag cttttgataa
    26641 tccccaaggt tctcaaggtg ccactgagtt attaaatact tttggtggcg gagccttgaa
    26701 acgattttct atttttgcaa tgggtattgt accctacatc actgcatcaa tcgtaatgca
    26761 attattacaa atggatattg tccctaaatt ctcagaatgg gcaaaacaag gtgaagtagg
    26821 tagaagaaag ttaaataacg ttactcgtta tttagcaatt tctttagcat ttatccaatc
    26881 tataggtatg gcattccaat ttaataatta tctcaaaggt gcgctgatta tcaatcagtc
    26941 aattatgagt tatttattaa tagcactagt tttgacagca ggaactgctt tcttaatatg
    27001 gcttggtgat caaatcactc agttcggtgt tggtaatggt atttctatta tcatatttgc
    27061 gggtatttta tcaacgttgc cagccagtct aattcaattt ggacaaactg cgttcgttgg
    27121 gcaagaagat acatcattag catggttgaa agtattagga cttttagtgt cactgatact
    27181 attaactgta ggtgctattt atgtacttga agccgttcgt aaaattccga ttcaatatgc
    27241 taagaaacaa actgctcaaa gattaggttc acaggcaact tatctacctt taaaagtgaa
    27301 ctcagccggt gttattcctg taatctttgc aatggcgttc ttcttactgc ctagaacatt
    27361 aacgttgttc tatccagata aagaatgggc acaaaacatt gcgaatgctg caaatccttc
    27421 aagtaatgtt ggtatggttg tatatattgt actaattata ctattcacat acttctatgc
    27481 atttgtacaa gttaatccgg aaaaaatggc tgataacctt aagaaacaag gtagctatgt
    27541 tccaggtatt agacctggtg aacaaaccaa aaaatatatt actaaagttt tatatcgttt
    27601 aacttttgta ggttctatct tcttagccgt tatttcaatt ctaccgatat tagcaactaa
    27661 gtttatggga ttaccacaat caattcagat tggtggtaca agtttactta tcgttattgg
    27721 tgtagcgatt gaaacaatga aatctttaga agctcaagta tctcaaaaag aatataaagg
    27781 ctttggtggt agataatttt aggagggcac ttatgaatat cattttgatg ggtttacctg
    27841 gcgcaggtaa aggaactcaa gcaagtgaaa ttgtcaagaa attcccaata ccccacattt
    27901 caactggtga catgttcaga aaagctataa aagaagaaac tgaattaggt aaagaagcta
    27961 agtcttatat ggaccgtggc gaattagttc ctgatgaagt gactgtaggt atcgttaagg
    28021 aaagaatttc tgaagacgat gcaaaaaaag gctttttatt agatggcttc ccaagaacaa
    28081 tcgagcaagc tgaggcatta aataatatta tgtctgagct tgacagaaac attgatgctg
    28141 tcatcaatat cgaagttccg gaagaagaat taatgaaccg tcttacaggt cgtcgaatct
    28201 gtgagtcatg tggtacaacg tatcatcttg tatttaatcc tccgaaggtc gaaggtattt
    28261 gtgatatcga tggtggtaaa ttgtatcaac gagaagatga taatcctgaa acggtagcta
    28321 atcgtttgag tgttaatatt aaacaatcta agcctatttt agatttctat gatcaaaaag
    28381 gtgtattgaa aaatattgat ggttcaaaag atattagcga cgttaccaaa gatgtcattg
    28441 atattttaga tcatttgtaa tagatcaact tttagcggtc tattttaagt gaggcatgat
    28501 ttaatcatat tagatagatg agagtggcaa ttaacgataa ttaattcccg taatttttgt
    28561 ttaacttata aaattaatca ctgatagatt cgtaacaata aagttgtcta ttgacgatta
    28621 ccttactatt gcaaaaaggg ggaagttaat caatggctaa acaagatgta attgaattag
    28681 aaggtactgt attagatact ttaccgaacg caatgtttaa agtagaatta gaaaatggtc
    28741 atgagatttt agctcacgta agtggtaaaa tcagaatgaa ttacattcgt attctacctg
    28801 gcgacaaagt aactgttgag atgtctccgt acgatttaac acgcggaaga attacttatc
    28861 gttataaata atcgtcactc cataatatag ggaggtataa aaatgaaagt aagaccatca
    28921 gtaaaaccta tttgcgaaaa atgtaaagtc attaaacgta aaggtaaagt aatggtaatt
    28981 tgtgaaaatc caaaacacaa acaaagacaa ggttaataaa agagaggtgt aaattaatat
    29041 ggcacgtatt gcaggagtag atattccacg tgaaaaacgc gtagttatct cattaactta
    29101 tatatacggt atcggtacgt caactgctca aaaaattctt gaagaagcta acgtatcagc
    29161 tgatactcgt gtgaaagatt taactgatga cgaattaggt cgcatccgtg aagttgtaga
    29221 cggttataaa gtcgaaggtg acttacgtcg tgaaactaac ttaaatatca aacgtttaat
    29281 ggaaatttca tcataccgtg gtatccgtca ccgtcgtggt ttaccagttc gtggtcaaaa
    29341 aacgaaaaac aacgcgcgta ctcgtaaagg accagttaaa acggtagcta acaagaaaaa
    29401 ataataggta aaggaggcaa attttaaatg gcacgtaaac aagtatctcg taaacgtaga
    29461 gtgaaaaaga atattgaaaa tggtgtagca cacatccgtt caacattcaa caacactatt
    29521 gtaactatca ctgatgagtt cggtaatgct ttatcatggt catcagctgg tgcattagga
    29581 ttcaaaggat ctaaaaaatc aacaccattt gcagcacaaa tggcttctga aactgcatct
    29641 aaatcagcta tggagcatgg tttaaaaaca gttgaagtaa cagttaaagg acctggtcca
    29701 ggtcgtgaat cagctattcg tgcattacaa tctgcaggtt tagaagtaac tgcgatcaga
    29761 gacgttactc cagtacctca taacggttgt cgtccaccaa aacgtcgtcg tgtataattt
    29821 atgatggtat tgttacaggt cactgagcaa acattttaaa ttaagtcgac gtatataagg
    29881 aggatattta aatgatagaa atcgaaaaac ctagaattga gacaattgaa attagtgaag
    29941 atgctaaatt cggtaagttc gttgttgaac cactagaacg tggctacggt actacactag
    30001 gaaactcctt acgtcgtatc ctactatctt cattaccagg tgcagccgtt aagtatattg
    30061 aaattgaggg agttttacat gaattctcag cagtagacaa tgtagttgaa gatgtttcta
    30121 caattattat gaacattaaa caattagcat tgaaaattta ctctgaagaa gataaaactt
    30181 tagaaattga tgtacgtgat gaaggcgaag taacagcaag cgacattaca catgatagtg
    30241 atgttgaaat tttaaaccca gagcttaaaa ttgcaacagt atctaaaggt ggtcacttaa
    30301 aaattcgtct agttgctaac aagggtagag gttacgcatt agcagaacaa aataatacta
    30361 gtgatttacc aattggtgta atccctgttg attcattgta ttcacctgtt gaacgtgtga
    30421 actatactgt tgaaaataca cgtgtaggtc aaagcagtga ttttgataaa ttaacattgg
    30481 atgtttggac taatggttca atcacaccac aagaatcagt ttcattagca gcaaaaataa
    30541 tgactgaaca cttgaatatc ttcgttggtc ttactgatga agcgcaaaac gctgaaatca
    30601 tgattgaaaa agaagaagat caaaaagaaa aagtattaga aatgtctatt gaagaattag
    30661 acttatctgt acgttcatat aactgcttaa aacgcgcagg aatcaattct gttcaagagt
    30721 tagctgacaa atctgaagct gacatgatga aagtgcgtaa tttaggtcgt aaatctttag
    30781 aagaagttaa atacaaatta gaagatttag gattaggatt aagaaaagaa gattgataaa
    30841 ggaggttaac tcatgggtta cagaaaatta ggtcgtactt ctgatcaacg taaagctatg
    30901 ttacgtgact tagctacatc acttattatt agtgaacgta ttgaaactac agaagctcgt
    30961 gcaaaagaag ttcgcagtgt tgttgagaaa ttaatcactt taggtaaaaa aggagattta
    31021 gcttctcgtc gtaatgcagc taaaacttta cgtaatgttg aaatcttaaa cgaagatgaa
    31081 actacacaaa ctgcacttca aaaattattt ggtgaaatcg cagagcgtta cacagaacgt
    31141 caaggtggtt acactcgtat ccttaaacaa ggccctcgtc gcggtgacgg tgctgaatca
    31201 gtaattatcg aattagtata agaatttata taaatacact aactactttt atataaagca
    31261 aatgagtgca acgataatgt ttgccacata caaatattgt ctagctcaga gtaccccatc
    31321 aactaaataa agatttaaag cgtgaactca acaaacttat gatggggtgc gcgctttttt
    31381 attgaataaa atatcggact tatgaacaga aatgttttta agtccgattt ctttatattt
    31441 acttttttga gtgcgaatct aaaaatatga tgaagctagt ttgatcaatt tcactagaac
    31501 tgaatgaagt gaaagtatta tcgttgaatc aaacgcgacc acccctatat tagtaaatca
    31561 tttattttat taacgtttat acataattgt tagagaggaa taaaagtcct tatcattagt
    31621 tgttaaacaa cactttaaag actagaaatg agtcagaaaa tactagtaat atatagagaa
    31681 cccagtcaat gtcatataca gcgatttata aatattgtaa aatatgatag atacacgaat
    31741 cgtaaaggag agagacaagt ggaggataag aattcagtta ttgtatttaa aaatgtttca
    31801 tttcaatatc aaagtgatgc atccttcaca ttgaaagatg tttcttttaa tatacctaaa
    31861 ggtcagtgga catctattgt tggtcataac ggttctggaa aatctacaat tgccaagtta
    31921 atgattggca tagagaaagt taaatctgga gaaatttttt ataataatca agctataact
    31981 gatgataatt ttgaaaagtt aagaaaagac ataggaattg tatttcagaa tccggataat
    32041 caatttgttg gttcaattgt aaaatacgat gtggcatttg gactcgaaaa tcatgcggtt
    32101 ccatatgacg aaatgcatag aagagtcagc gaagcactta aacaagttga tatgttagaa
    32161 cgtgcagatt atgaacctaa tgcattatcg gggggacaga agcagcgtgt ggctatagca
    32221 agtgtattag cacttaaccc ctctgtcatt atattagatg aggcgacttc tatgttagat
    32281 cctgatgcac gtcaaaattt attggattta gtgagaaaag ttaaatcaga acataatatt
    32341 acaatcattt ctattacgca tgatttatct gaggcgatgg aagcagatca tgttatcgtt
    32401 atgaataaag ggactgtcta taaagaaggc acagcgactg aaattttcga caatgcagaa
    32461 gagttaacaa gaataggtct agatttgcca ttcccaatca aaataaatca aatgctggga
    32521 caccaaacat cattcttaac ttatgaaggg ctggtggatc aactatgact atacggtttg
    32581 acaatgtaag ttatacctat caaaaaggga caccatatca gcatcaagct attcatgatg
    32641 ttaatacaga atttgaacaa ggtaaatatt acgccatcgt tggacaaacg ggtagtggta
    32701 aatcaacgtt gatacaaaat attaatgcgc tgttaaagcc gactactggg acagttacag
    32761 ttgatgacat tactattaca cataagacca aagataaata tattagacct gtaagaaaaa
    32821 gaattggaat ggtatttcaa tttcccgaat ctcaattatt tgaggacaca gtagagcgtg
    32881 aaatgatatt tggacctaaa aactttaaaa tgaatttaga tgaagccaaa aactatgccc
    32941 atcgtctgtt gatggattta ggcttttcaa gagatgtaat gtcacaatca ccatttcaaa
    33001 tgtcaggtgg acaaatgcgt aaaatagcga ttgtatcgat attggcaatg aatcctgata
    33061 ttatcgtggt tgatgaacct acagcaggac ttgatccaca aagtaaacga caagtaatga
    33121 gattactaaa gtcactacaa acagatgaaa ataaggcaat tatcctaatt tcacatgata
    33181 tgaatgaagt cgcgcgttat gcagatgaag tcatcgttat gaaagaaggt agtatagtgt
    33241 cgcaaacatc accaaaagag ctcttcaaag ataaaaaaaa attggcggat tggcatattg
    33301 gtttgccaga aattgttcag ttacaatatg actttgaaca aaaatatcaa acaaaattaa
    33361 aagatattgc cttaactgaa gaagcatttg taagcttgta taaggagtgg caacatgaaa
    33421 aataaattga ttatagggcg atatttacca attaattctt ttgttcatca tcttgatcca
    33481 agagcaaagt taatgtttgt ttttcttttt attatatta
//
LOCUS       DAKTIP010000008        32590 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAKTIP010000008 DAKTIP010000000
VERSION     DAKTIP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 32590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.008"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            318..7133
                     /gene="sasA"
                     /locus_tag="O0305_000294"
     CDS             318..7133
                     /gene="sasA"
                     /locus_tag="O0305_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCY0273480.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSAFTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSDSTSLSTSLSDSTSDSKSDSLSTSMSTS
                     DSISTSKSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTS
                     TSLSDSTSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQS
                     ESTSTSTSLSDSTSISKSTSQSGSVSTSASLSGSESESDSQSISTSASESTSESASTS
                     LSDSTSTSNSGSASTSTSLSNSASASESDLSSTSLSDSTSASMQSSESDSQSTSASLS
                     DSLSTSTSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGS
                     ASTSTSTSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDS
                     TSASTSGSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDS
                     KSMSGSTSVSDSGSLSVSTSLRKSESVSESSSLSCSQSMSDSVSTSDSSSLSVSTSLR
                     SSESVSESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTS
                     TSDSDSLSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNS
                     QFDSMSISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGS
                     TSVSDSSSTSTSTSLSDSMSQSQSTSTSASGSLSTSISTSMSMSASTSSSQSTSVSTS
                     LSTSDSISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSGS
                     TSTSISESLSTSGSGSTSVSDSTSMSESNSSSVSMSQDKSDSTSISDSESVSTSTSTS
                     LSTSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTS
                     TSRLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTL
                     LVGLGLMKRKKKKDENDQDDSQA"
     gene            7263..8474
                     /gene="secY2"
                     /locus_tag="O0305_000295"
     CDS             7263..8474
                     /gene="secY2"
                     /locus_tag="O0305_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCY0273481.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8485..10038
                     /gene="asp1"
                     /locus_tag="O0305_000296"
     CDS             8485..10038
                     /gene="asp1"
                     /locus_tag="O0305_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCY0273482.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            10028..11596
                     /gene="asp2"
                     /locus_tag="O0305_000297"
     CDS             10028..11596
                     /gene="asp2"
                     /locus_tag="O0305_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCY0273483.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11574..12533
                     /gene="asp3"
                     /locus_tag="O0305_000298"
     CDS             11574..12533
                     /gene="asp3"
                     /locus_tag="O0305_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCY0273484.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQYDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12523..14913
                     /gene="secA2"
                     /locus_tag="O0305_000299"
     CDS             12523..14913
                     /gene="secA2"
                     /locus_tag="O0305_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCY0273485.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTVSLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14931..16439
                     /gene="gtfA"
                     /locus_tag="O0305_000300"
     CDS             14931..16439
                     /gene="gtfA"
                     /locus_tag="O0305_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCY0273486.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16432..17790
                     /gene="gtfB"
                     /locus_tag="O0305_000301"
     CDS             16432..17790
                     /gene="gtfB"
                     /locus_tag="O0305_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCY0273487.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFALFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            18032..19939
                     /gene="sasF"
                     /locus_tag="O0305_000302"
     CDS             18032..19939
                     /gene="sasF"
                     /locus_tag="O0305_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCY0273488.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ITKEILSKQDLLDKVDKAIRQIEQLKQLSASSKEHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEAVKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSDLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            20107..20667
                     /locus_tag="O0305_000303"
     CDS             20107..20667
                     /locus_tag="O0305_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0273489.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20934..22793)
                     /locus_tag="O0305_000304"
     CDS             complement(20934..22793)
                     /locus_tag="O0305_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCY0273490.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKAQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            23004..25985
                     /locus_tag="O0305_000305"
     CDS             23004..25985
                     /locus_tag="O0305_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0273491.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(26097..27035)
                     /gene="manA"
                     /locus_tag="O0305_000306"
     CDS             complement(26097..27035)
                     /gene="manA"
                     /locus_tag="O0305_000306"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCY0273492.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(27049..29001)
                     /locus_tag="O0305_000307"
     CDS             complement(27049..29001)
                     /locus_tag="O0305_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCY0273493.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMGSTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWDSLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(29078..30952)
                     /locus_tag="O0305_000308"
     CDS             complement(29078..30952)
                     /locus_tag="O0305_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0273494.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAKHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRHVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(31192..31650)
                     /locus_tag="O0305_000309"
     CDS             complement(31192..31650)
                     /locus_tag="O0305_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273495.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHK
                     KTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDS
                     NSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN"
     gene            31908..32435
                     /gene="isaB"
                     /locus_tag="O0305_000310"
     CDS             31908..32435
                     /gene="isaB"
                     /locus_tag="O0305_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCY0273496.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT        11330 a         5118 c         6062 g        10080 t
ORIGIN      
        1 tagttgtaaa ttaactattt ataaataaaa ggtggatggt atagatatat taatacaata
       61 attattttaa aaacgaaaca attataatga ttaaacataa agccaattag ccaaatttat
      121 ataaaaaaag attatacgca taaacgagaa aaagtaattt taaaaatatg aaaacgatta
      181 catttagacg aataaaaatg aatttttaca aattttaaca aaatgagtat ttttgtatca
      241 aaaaagagtt gtattaaatt gaatagtggc atatattata atctaatgaa tgtaaagata
      301 atacaaggag ttattacatg agtaaaagac agaaagcatt tcatgacagc ttagcaaacg
      361 aaaaaacaag agtaagactt tataaatctg gaaaaaattg ggtaaaatcc ggaattaaag
      421 aaatagaaat gttcaaaatt atggggctac catttattag tcatagttta gtgagtcaag
      481 ataatcaaag cattagtaaa aaaatgacgg gatacggact gaaaactacg gcggttattg
      541 gtggtgcatt cacggtaaat atgttgcatg accagcaagc ttttgcggct tctgatgcac
      601 cattaacttc tgaattaaac acacaaagtg aaacagtagg taatcaaaac tcaacgacaa
      661 tcgaagcatc aacatcaaca gccgattcca caagtgtaac gaaaaatagt agttcggtac
      721 aaacatcaaa tagtgacaca gtctcaagtg aaaagtctga aaaggtcact tcgacaacta
      781 atagtacaag caatcaacaa gagaaattga catctacatc agaatcaaca tcctcaaaga
      841 atactacatc aagttctgat actaaatctg tagcttcaac ttcaagtaca gaacaaccaa
      901 ttaatacatc aacaaatcaa agtactgcat caaataacac ttcacaaagc acaacgccat
      961 cttcggtcaa cttaaacaaa actagcacaa cgtcaactag caccgcacca gtaaaacttc
     1021 gaactttcag tcgcttagct atgtcaacat ttgcgtcagc agcgacgaca accgcagtaa
     1081 ctgctaatac aattacagtt aataaagata acttaaaaca atatatgaca acgtcaggta
     1141 atgctaccta tgatcaaagt accggtattg tgacgttaac acaggatgca tacagccaaa
     1201 aaggtgctat tacattagga acacgtattg actctaataa gagttttcat ttttctggaa
     1261 aagtaaattt aggtaacaaa tatgaagggc atggaaatgg tggagatggt atcggttttg
     1321 ccttttcacc aggtgtatta ggtgaaacag ggttaaacgg tgccgcagta ggtattggtg
     1381 gcttaagtaa cgcatttggc ttcaaattgg atacgtatca caatacatct aaaccaaatt
     1441 cagctgcaaa ggcgaatgct gacccatcta atgtagctgg tggaggtgcg tttggtgcat
     1501 ttgtaacaac agatagttat ggtgttgcga caacgtatac atcaagttca acagctgata
     1561 atgctgcgaa gttaaatgtt caacctacaa ataacacgtt ccaagatttt gatattaact
     1621 ataatggtga tacaaaggtt atgactgtca aatatgcagg tcaaacatgg acacgtaata
     1681 tttcagattg gattgcgaaa agtggtacga ccaacttttc attatcaatg acagcctcaa
     1741 caggtggcgc gacaaattta caacaagtac aatttggaac attcgaatat acagagtctg
     1801 ctgttacaca agtgagatac gttgatgtaa caacaggtaa agatattatt ccaccaaaaa
     1861 catattcagg aaatgttgat caagtcgtga caatcgataa tcagcaatct gcattgactg
     1921 ctaaaggata taactacacg tccgtcgata gttcatatgc gtcaacttat aatgatacaa
     1981 ataaaactgt aaaaatgacg aatgctggac aatcagtgac atattatttt actgatgtaa
     2041 aagcaccaac tgtaactgta ggcaatcaaa ccatagaagt gggtaaaaca atgaatccta
     2101 ttgtattgac tacaacggat aatggtactg ggactgtgac aaatacagtt acaggattac
     2161 caagcggatt aagttacgat agtgcaacga attcaatcat tgggacacca acaaaaattg
     2221 gtcaatcaac agtgacagtt gtgtctactg accaagcaaa taacaaatcg acgacaactt
     2281 ttacaataaa tgttgtggat acgacagcac caacagtgac accaatagga gatcaatcat
     2341 cagaagtgta ttcaccaata tccccgatta aaattgctac gcaagataac agtggaaatg
     2401 cggtgacgaa tacagtgact ggattgccat ccggactaac atttgatagt acaaataata
     2461 ctattagtgg tacaccaaca aacattggta caagtactat atcaatcgtt tctacagatg
     2521 cgagcggtaa caaaacgacg acaactttta aatatgaagt aacaagaaat agcatgagtg
     2581 attccgtatc aacatcagga agtacacaac aatctcaaag tgtgtcaaca agtaaagctg
     2641 actcacaaag tgcatcaacg agtacatcag gatcgattgt ggtatctaca tcagctagta
     2701 cctcgaaatc gacaagtgta agcctatctg attctgtgag tgcatctaag tcattaagca
     2761 catctgaaag taatagtgta tcaagctcaa caagcacaag tttagtgaat tcacaaagtg
     2821 tatcatcaag catgtcggat tcagctagta aatcaacatc attaagcgat tctatttcaa
     2881 actctagcag tactgaaaaa tccgaaagtc tatcaacaag tacatctgat tcattgcgta
     2941 catcaacatc actcagtgac tcattaagta tgagtacatc aggaagcttg tctaagtcac
     3001 aaagcttatc aacgagtata tcagggtcgt ctagtacatc agcatcatta agtgacagta
     3061 catcgaatgc aattagtaca tcaacatcat tgagcgagtc agctagcacc tcggactcta
     3121 tcagtatttc aaatagcata gccaactctc aaagtgcgtc aacaagcaaa tcagattcac
     3181 aaagtacatc aatatcatta agtacaagtg attcaaaatc gatgagtaca tcagaatcat
     3241 tgagcgattc gacgagcaca agtggttctg tttctggatc actaagcata gcagcatcac
     3301 aaagtgtctc aacaagtaca tcagactcga tgagtacttc agagatagta agtgactcta
     3361 tcagtacaag tgggtcatta tctgcatcag acagtaaatc aatgtccgta agtagttcaa
     3421 tgagcacgtc tcagtcaggt agtacatcag aatcattaag tgattcacaa agtacatctg
     3481 attctgatag taagtcatta tcacaaagta ctagtcaatc aggttcaaca agtacatcaa
     3541 cgtcgacaag tgcttcagta cgtacttcgg aatcacaaag tacgtctggt tcaatgagtg
     3601 caagtcaatc cgattcaatg agcatatcaa cgtcgtttag tgattcaacg agtgatagca
     3661 aatcagcatc aactgcatca agtgaatcaa tatcacaaag tgcttttacg agcacatctg
     3721 gttcggtaag tacttcgaca tcgttaagta caagtaattc agaacgtaca tcaacatcta
     3781 tgagtgattc cacaagctta agtacatcag agtctgattc aataagtgaa tcaacgtcaa
     3841 cgagcgactc tataagtgaa gcaatatctg cttcagagag cacgtttata tcattaagtg
     3901 aatcaaatag tactagcgat tcagaatcac aaagtgcatc tgccttttta agtgaatcat
     3961 taagtgaaag tacgtctgaa tcaacatcag agtcagtgag tagttcgaca agtgagagta
     4021 cgtcattatc agacagtaca tcagaatctg gtagcacatc aacatcatta agtaattcaa
     4081 caagtggtag tacgtccatt tcaacatcga caagtatcag tgaatcaacg tcaacgttta
     4141 agagcgagag tgtttcaaca tcactgagta tgtcaacgag tacaagtttg tctgactcta
     4201 caagtttgtc aacatcatta agtgattcca caagtgatag taagtctgat tcattaagta
     4261 catcaatgtc gacaagtgat tcaatcagta caagtaaatc tgattccatt agtacatcca
     4321 catcattaag tggttctaca agtgaaagtg aatccgactc aacatcatca agtgaaagta
     4381 aatccgattc aacatcaatg agcataagta tgtctcaatc aacatcagga agtacaagta
     4441 cgtcaacgag tacaagtttg tctgactcaa cgagtacatc attgtcacta agtgcctcaa
     4501 tgaatcaaag cggagtagac tcaaactcag caagccaaag tgcctcaaac tcaacaagta
     4561 caagcacgag cgaatccgat tcacaaagca catcatcata tacaagtcag tcaacaagcc
     4621 aaagtgaatc cacatcgaca tcaacgtcac taagcgattc aacaagtata tctaaaagta
     4681 cgagtcaatc aggttcggta agcacatcag cgtcattaag tggttcagag agtgaatctg
     4741 attcacaaag tatctcaaca agtgcaagtg agtcaacatc agaaagtgcg tcaacatcac
     4801 tcagtgactc aacaagtaca agtaactcag gatcagcaag tacgtcaaca tcgctcagta
     4861 actcagcaag cgcaagtgaa tccgatttgt cgtcaacatc tttaagtgat tcaacatctg
     4921 cgtcaatgca aagcagtgaa tccgattcac aaagcacatc agcatcatta agtgattcgc
     4981 taagtacatc aacttcaaac cgcatgtcga ccattgcaag tttatctaca tcggtaagta
     5041 catcagagtc tggctcaaca tcagaaagta caagtgaatc cgattcaaca tcaacatcat
     5101 taagcgattc acaaagcaca tcaagaagta caagtgcatc aggatcagca agtacatcaa
     5161 catcaacaag tgactctcgt agtacatcag cttcaactag tacttcgatg cgtacaagta
     5221 ctagtgattc acaaagtatg tcgctttcga caagtacatc aacaagtatg agtgattcaa
     5281 cgtcattatc tgatagtgtt agtgattcaa catcagactc aacaagtgcg agtacatctg
     5341 gttcgatgag tgtgtctata tcgttaagtg attcgacaag tacatcaaca tcggctagtg
     5401 aagtaatgag cgcaagcata tctgattcac aaagtatgtc agaatctgta aatgattcag
     5461 aaagtgtaag tgaatctaat tctgaaagtg actctaaatc gatgagtggc tcaacaagtg
     5521 tcagtgattc tggctcattg agcgtctcaa cgtcattaag aaaatcagaa agtgtaagcg
     5581 agtcaagttc attgagttgc tcacaatcga tgagcgattc agtaagcaca agcgattcgt
     5641 catcattaag tgtatcgacg tcactaagaa gttcagaaag cgtgagtgaa tctgattcat
     5701 taagtgattc aaaatcaaca agtggttcga cttcaacaag tacatctggt tcattgagta
     5761 cctcaacatc attaagtggt tcagaaagcg taagcgagtc tacctcgcta agtgattcaa
     5821 tatcaatgag tgattctact agtacaagtg actccgactc attaagtgga tcaatatctt
     5881 taagtggttc cacaagtctt agcacttcgg attcattaag tgattcaaaa tcattgagta
     5941 gctcgcaaag tatgagtgga tcagaatcaa cgtcaacaag tgtgagcgat tcgcagtcaa
     6001 gctcaacaag taatagtcaa tttgactcta tgagcatcag tgcatcagaa agcgactcaa
     6061 tgtctacaag tgattcgtct agcatcagtg gatcaaattc aacgagtaca tcactttcaa
     6121 catctgactc aatgagcgga agcgtatcag tttcaacatc gacaagttta agtgactcaa
     6181 tatcaggttc aacaagtgta agtgactcga gctcaacaag cacatctaca tcattaagtg
     6241 attcaatgtc acaaagccag tcaacaagta caagtgcatc tggttcctta agtacatcga
     6301 tatcaacatc aatgtcaatg agtgctagta catcgtcatc acaaagcaca tcggtgtcga
     6361 catcattatc aacatcagac agtatcagtg attctacttc aataagtatc agtggttcac
     6421 aaagtacagt agaatcagaa tctacaagtg attcaacttc tatcagtgac tcagaatcat
     6481 tgagtacatc agattcagac tcgacatcga caagtacatc ggactcaaca agtggttcaa
     6541 cttcaacaag catatctgaa tcattaagta cgtctggttc aggttcaacg agcgtatctg
     6601 actcaacatc aatgagtgaa tctaattcat cgagtgtttc aatgtcacaa gacaaatccg
     6661 actcaacatc aattagtgac tcagaatcag tgtcaacaag cacatcaacg tcattgagca
     6721 catccgattc gacaagcaca tccgaatcac tgagtacatc tatgtctggt tcacaaagca
     6781 tttctgactc aacatcaaca agtatgtccg gctcaacaag tacatccgaa tctaactcaa
     6841 tgcatccgtc agactcaatg agtatgcatc atactcacag cacgagcaca tctcgcttat
     6901 caagtgaagc aacaacgagc acgagtgaat ctcagtctac attaagtgca acatctgaag
     6961 tgactaaaca taatggcaca ccagcacaaa gtgaaaaaag attgccagat acaggtgact
     7021 caataaaaca aaatggatta ctaggtggcg ttatgacatt attagttggt ttaggtttaa
     7081 tgaagagaaa gaaaaagaaa gatgaaaatg atcaagatga ttctcaagca taaattgaat
     7141 ttatagctgg ctaaaattaa aaagactgag gcaccaatca ctagaaaaat cgatatagga
     7201 ttttaataat tagtgatgaa cccttagtct ttttaatgtt tttgatataa aagaggtgca
     7261 acatgttaaa acttttacaa caatacgaat ataaaattat atataaacgt atgctataca
     7321 catgttttat cttgtttatt tatatattag gcacaaacat ttccatcgtt tcctataacg
     7381 atatgcaagt aaaacatgag tcatttttta aaatagctat ttctaatatg ggtggagacg
     7441 ttaatacttt aaacattttc actttaggat taggtccgtg gttaacatca atgattatct
     7501 tgatgctgat ttcttatcgg aatatggata aatacatgaa acaaacaagt cttgagaagc
     7561 attataaaga gcgcatctta acattaattt taagtgttat tcaaagttat tttgtgattc
     7621 atgaatatgt ttcgaaagaa cgtgttcatc aagacaatat ttacttaaca atattgattt
     7681 tggttactgg aacgatgtta ttagtttggt tagctgacaa aaatagtcgc tacggcatcg
     7741 caggaccaat gccgattgta atggttagta ttattaaatc aatgatgcat caaaaaatgg
     7801 aatatataga tgcaagtcat attgtgattg cgttgttaat catccttgtg ataatcacat
     7861 tgtttatctt attatttatt gaattagtag aagtgagaat accatatatc gatttaatga
     7921 acgtttccgc aacaaatatg aaatcttatt tatcttggaa agttaaccct gcaggcagta
     7981 ttactttaat gatgagtatt tcagcttttg ttttcttaaa aagtggcatt cattttattt
     8041 tatctatgtt taataaaagc atatcagatg acatgccaat gctgacattt gatagtccag
     8101 taggtatttc agtatattta gtgattcaaa tgttattggg ttatttttta tcgagatttt
     8161 taatcaatac taaacaaaaa tccaaagatt tcttaaagag tggcaattat ttttcaggag
     8221 ttaaacctgg taaggataca gaacgttatt taaattatca agcacgacgc gtatgttggt
     8281 ttggattggc attagttaca gtcattattg gtataccgct ttatttcaca ttgtttgtac
     8341 cgcatttatc tactgaaatt tatttctcag tacaactgat tgtattagtt tacatcagta
     8401 ttaatattgc agaaacaatt cgtacatatt tatattttga taaatataag ccatttttaa
     8461 accagtattg gtaaggaggt aattatgaaa tactttattc cagcttggta cgatgaccaa
     8521 cgatggtggc aagacacgac tgtgccgtat tatcaactac aaaataagac ggaatttgac
     8581 gatatgatta gtttaatggg aatgcacctt gaaaatgact tagattatca actgattgtt
     8641 ctcaatcatg caccaaattt aagaacattt ttacatcgat atgacttata tgagacaaag
     8701 tattcgtctg tgtttgatga aattcaagga ttcagtcacc atgcgccaca agcgattaat
     8761 tatcatcact taaaatggcc ggatgatgtt gagtttgtgt acacaccgta tttattaaaa
     8821 tgtgtgacga gtgaacagac ctatacaaat atttatttta gtcaagaagg gtattcaatt
     8881 tggtttgaag aatttgaaag agatcagtta caacggcgtt atatttttga tgacagaggt
     8941 tatttatcag cgatacgtta ttttgatgat cagggagagg cttcttacca agaatattta
     9001 acgattaatg gagattgtgt acttcatgaa gatttgaaaa atggcagagt cactgtttca
     9061 aaaagatatc aacatcacta tcaacaaaca gaatataaca atatggctca actaattgaa
     9121 gaaaaatttc aagcaatgat tgcacaacaa atacatgaag atgatcatgt gattgtggct
     9181 tcagatgcta ggcacaatcg acaaatagcc aatcatattc cagcgaaatc attaagttat
     9241 tcatttttta aaaatagaaa tgaaactgtg tcagatgagg aatatcaatc tatcgtaaag
     9301 aatgcccatt taattgttga tagtgtgcaa ctagaacgtg atttaattag tcatcaagag
     9361 aagtatcagc gggagaatac aatgattcga atcacaccat ttgaaacgag acaatcacct
     9421 aatataagta gtcaattgat ggaaacattt ataggtgtat ggatagatgg tatgagtgac
     9481 gctgatttgc aacaaatgat gcaacgactt gtggattata tcgcacagga agattattac
     9541 cgtttaattt tattatcgcg ccatcaaaat gacataccga tgtggcttcg tgaatgtatt
     9601 acgtcggtaa atgaggaata ccaagctaaa cagaatgcgg atgttaatgt ttcagcatta
     9661 atgacacctg aagatcaaga tgacatcatt gctgttaaga cgatacatgc tgaacatgat
     9721 gttgtagaag cattgcggac gttgcgactt gtgatagata tgtcaaaaga acctgacttg
     9781 tatttacaaa ttagtgcaat tagcgctggg attccacaaa ttaatggtca acaaacagat
     9841 tacgtctctg attatgacaa tggccgtatt ataaatacag ttgatgaatt agatgatgcg
     9901 ttaaattatt atttatttta tttgaaaaat tggaattatg cgtacgccta ttctttaaaa
     9961 ttaatagatg catatgcttc taagaatatt attaatcagc tcgatgagtt aatagaaggt
    10021 gaaaatgatg ccacgtaaat ttagagtttt gcaaattgga ggagacgatt tagaacctat
    10081 ttttcaacac aaaaaaggtg tgagttggga ttacttcgat attggattgt ttgaatttga
    10141 tagtggttat gtagaggcta ttgaagcgat tgttgaagca gaagggcgct ttgattttat
    10201 ctatattcaa gcaccatact cggagacatt aacgaattta ttacaaatga taagcgaacc
    10261 atacaatacg tatgttgatg aatcattttg gtcagttgaa tatgaacaag acgaaaatgt
    10321 ccaaaaatac gttgttcaac cattacatta ccggaatatt gaagaacgta ataataaatt
    10381 agaggcagtt agcttctcag gacagtatgg agataaggtt tctcctaagt tagcacttgt
    10441 gcatccgaat tttaaaggag atgtcgttta ccaaggtaat tcagagctca cattgagtgg
    10501 agaatttggg aaagaattta aacctatcgc atcttggcaa aataatctcg tttacgataa
    10561 agataaagtc attcaaatat ggccagaatt tgatattgat ggtgcggtcg agttgcaata
    10621 tacatttaga ttgattcaga ctggcgctga tggtgcatta attgaacaaa tcgtattgac
    10681 tgatgatatg ttagacagtc ctttagagat acctgcgaaa ccatttgatg cttatataag
    10741 tgtaactgtt aaggcgcgtg ggaacgggac ggtacattta ggacctatac acaaacgatg
    10801 gtctagatta gacatgggtc aatttttact tggtggtagt cgtttcgtag atagtcaacg
    10861 acaggaattt atttattatt tccaccctgg tgatatgaaa ccgccactaa acgtatattt
    10921 tagtggttat cgaacagcgg aaggtttcga aggatattat atgatgaaac gtatgaatgc
    10981 accgttttta cttatcggcg atcctcgtgt tgaaggtggt agtttttata tcggttcatc
    11041 tgaatatgaa caaggtatta tcaatgttat tgacgagaca ttagaaaagc tgaattttaa
    11101 atcacatgaa ttaatattat ctggtttatc tatgggttca tttggtgcct tgtattatgg
    11161 tgctcagtta aatcctcagg caattattgt tggtaaacca cttgttaata ttggaacgat
    11221 tgctgaacat atgcgattac ttagaccaga agagttcggt acagcattag acgtcctggt
    11281 gtctaatgaa ggtgatacat ctcaagcgtc gatacaggca ttaaatcaaa aattttggca
    11341 aacatttcaa aagaaatcat tgtcacaaac tgtttttgcc atagcatata tgcaacatga
    11401 tgattatgat ccacatgcat ttcaagagct attacctgta ttgacagcac accaagcaag
    11461 agtgatgaat cgcagtattc ctgggcgaca taatgatgat tcacctacca ttgcaagttg
    11521 gtttgttaat ttttacaaca ttattttgga agacaagttt gggagagttc aacatgctga
    11581 aaaacaaaac atttaaagtt acttggaatt atatttcaca aactacattt atgtatggct
    11641 ctaaagtatc attttcaaat ggtgaagtga cttttataaa tcctttgatg ccctctggat
    11701 taccgattca tgagtggttg atgttaaagc aattttcaaa atataaaagt gcgccaagtt
    11761 taccaatttt gcgtagagga cagcattata aattgcattt tgattttgat gcaacgcctg
    11821 ctggttcagt gtattttatc atcatttttt ataataaaaa tggcacaaaa ctaagtactg
    11881 agattgtaaa gtcaaatagt attacgattc aatatcctga tgaagcctat gcttataaaa
    11941 taaaaatgat gaatgcagca tcgacgagtc ttatatttag atgtttaaca atcacagaga
    12001 tgacgcatca atatgattta gagtataaaa gcatgcgggt gacgaagata ggcgatgacc
    12061 aatatggaaa tgatatgatc aatgtcatta ttgcagaacc atctgacacc tatccaacta
    12121 tttcgaatga ttttttgaaa ctgtttggtc atgtttggct cgttgaacgt tggatggacg
    12181 acaatatcga gggaaatatt aagcaactaa agaatgattt gcaaagtcaa gataccctaa
    12241 aggctattaa tttgattagt tatgggtcaa agtcaaatgt ttttgcaact tatgtcgctc
    12301 agcatttggg ttgcaaagtg tatcgtacaa gccatgaaga tgatgattta aaaggatggc
    12361 ttacagaaca tgtcccagga aatcatgaac taaaagatac aaatgtcgag gtttatttta
    12421 aagaagagca agacaaacat ctaaattata tgtcacgttt aatgaatcca gtacgatttt
    12481 tagattactt agatgtatcg aaactgaatg gtggggaagt aaatgaaaca taaactagat
    12541 gttacgataa acgaattaag actaaagtcg attagaaaaa tagtgaaacg catcaataca
    12601 tggagcgatg aagttaaatc atattctgat gatgcgttaa aacaaaagac aatagaattt
    12661 aaagaacgtt tagcatcagg agttgataca ctagatacat tgttacctga agcttatgca
    12721 gtggcacgtg aagcgagctg gagagtatta ggtatgtatc ctaaagaagt tcagttaatc
    12781 ggagcgattg tattgcatga aggtaacatt gcggagatgc aaacaggcga aggtaaaaca
    12841 ttaacggcaa cgatgccttt atatttaaat gctctttcgg gaaaaggaac atatttaatt
    12901 acaacgaatg attatttagc gaagcgagat tttgaagaga tgcaaccatt atatgaatgg
    12961 ttaggtttaa ctgtttcatt aggttttgtt gatattgtgg attatgagta tcaaaaaggg
    13021 gaaaaacgta atatatatga acatgatatt atatatacaa ctaatggacg tctcggtttt
    13081 gattatttaa ttgataattt ggctgatagt gcggaaggga aatttttacc acaattaaat
    13141 tacggtatta ttgatgaagt ggattcaatc attttagatg ctgctcaaac accattagtt
    13201 atttcgggtg caccaagatt acaatcaaat ctatttcata ttgtgaaaga gtttgtagat
    13261 acattgattg aagacgtgca ttttaaaatg aagaagacca aaaaagaaat atggctgtta
    13321 aatcaaggta ttgaagcggc acaatcatac tttaatgttg aagatttata tagcgaacaa
    13381 gcgatggtcc tagtgcgtaa tattaattta gcactgcgcg cacaatattt gtttgaatct
    13441 aatgtcgatt actttgtata taatggtgat attgttttaa ttgaccgtat tacaggtcgt
    13501 atgttaccgg gaactaagtt gcaagctgga cttcaccaag ctattgaagc gaaagaaggt
    13561 atggaggttt caacagataa aagtgttatg gcaacaatta ccttccagaa tttatttaaa
    13621 ctttttgaat cattttcagg tatgacagct acaggaaaat taggcgaatc agagttcttt
    13681 gatttgtatt caaaaatagt cgtacaagta ccaactgata aagcgattca acgtatcgat
    13741 gaaccagata aagtgtttcg ttcagttgat gagaaaaaca tcgcgatgat tcatgatata
    13801 gttgaacttc atgaaacggg gcgaccggtt ttactcataa cgagaactgc tgaagcggct
    13861 gaatactttt cgaaagtatt attccaaatg gatattccta ataatttact cattgcgcaa
    13921 aatgttgcaa aagaagcgca gatgatagct gaagcagggc aaattggttc catgactgtt
    13981 gcgactagta tggcaggtcg aggcacagat attaaacttg gtgaaggtgt cgaagcatta
    14041 ggtggattag ctgttattat tcatgaacat atggaaaata gccgtgtaga caggcaatta
    14101 cgtggtcgtt ctggtagaca aggggatccg ggatcatctt gtatatatat ttcactagat
    14161 gattatttag ttaagcgatg gagcgatagt aatttagcgg aaaataatca attatattca
    14221 ttagatgcac aacgattatc gcaaagtaat ttgtttaatc gcaaagttaa gcaaattgta
    14281 gttaaagcgc agcgtatctc ggaagaacaa ggggttaaag ctcgtgaaat ggctaatgaa
    14341 tttgaaaaaa gcattagtat acagcgagat cttgtatacg aggaacgcaa tcgagtttta
    14401 gaaatagatg atgctgagaa tcaagatttt aaagcgttag ctaaagatgt atttgaaatg
    14461 tttgtaaatg aggaaaaggt gctaacaaaa tcgcgtgttg tcgagtatat ttatcaaaat
    14521 ttaagtttcc aatttaataa agatgtggct tgtgttaatt ttaaagataa gcaagcagta
    14581 gtgacatttt tattagagca atttgaaaag caattagctt tgaatcgtaa aaacatgcaa
    14641 agtgcatatt attataatat tttcgttcaa aaagtctttt tgaaagcaat tgattcatgt
    14701 tggttagaac aagtcgacta tttacaacaa ttaaaagcaa gcgttaatca acgacaaaat
    14761 gggcaacgta acgctatttt tgaataccat agagttgcct tagattcatt tgaagttatg
    14821 acacgaaata tcaaaaaaag aatggtgaaa aatatttgtc aaagcatgat tacatttgat
    14881 aaagagggta tgccagttat acattttcca taaatgaaaa ggggtagcgc atgacaatat
    14941 ataatataaa ctttggtata ggctgggcga gtagcggtgt tgaatatgca caagcttacc
    15001 gtgctaaatt attgagacgt acaaatcaag acactaaatt tgtgttttta gattttatac
    15061 agtcagagaa catacagaca ttaacgcaaa acattgggtt taaagatcaa gaagttattt
    15121 ggttgtatca gtattttagt gatatttctt tagcaccaac aacgtacacg ttagatgaca
    15181 ttgaacgtga gcttggtcat gacattagtt ttagagaaag aaatggtaag atagtccgcc
    15241 tatattttaa tgggaaatca tcatttgtaa catgttatct gcaaaatgaa caaaaagata
    15301 ttgtggatcg tgcagaattt gtaattaata gtatgttagt acgtaaagat ttttatagtt
    15361 atacgcgcat attttctgag tattatgcac cagctgataa taaagcgaaa ttatatatgc
    15421 gacaatttta taatcaagat ggttccatcg cttataacga atatattgat ggagacagct
    15481 ctatatatgt gtttgaagat gcggtgttgt ataacaaaac ggaatttatt gcatactttt
    15541 tacaacgatt gaatttaacc cgagacgata ttgtcatatt agaccgcgct tctgatattg
    15601 gtcaggctgt tttgcagcat aaaggtgata gtaaagttgg tgttgtgatt catgctgatc
    15661 attatagtaa taatatgatg agtgagcagc atatactatg gaacaattat tatgaatatc
    15721 aattttcaaa agctaaatac attgatttct ttattacagc aacagatatt caaaatcata
    15781 tggtttgtcg acaatttgag caatatcaag gttatcgccc acgtgtttat actattcctg
    15841 tgggaagcat tgatgcatta tcatatccaa cattatcaag aaagccatat gcgatgatta
    15901 gtgcatctcg tttagcgaat gagaaacata ttgattggct tgtcaaagcg gttatcgtgg
    15961 ctaaacgaca agtgccagaa ttaacatttg atatttatgg agaaggtagc gagaagacgc
    16021 gactgcgtaa aattattgat acacaccgcg cacaagatta tattcgttta ctagggcatg
    16081 ttaaacttga tgaaatttat aatgattatg agttatttct ttctgcatca actagcgaag
    16141 gttttgggtt aacactaatg gaagcggtag gctcaggact aggtatgatt ggattaaatg
    16201 tgaattatgg taacccaacc tttattcgag atggggaaaa tggctacttg gtaccttttg
    16261 ataccgacga agatagtgtg gatgatgtta tcgcaaaact agctcatgcc attgtaatgt
    16321 attttaacaa tggtccccag acaccacatg acatctctta tgaagtggca cagcaattta
    16381 tgactcaaga tattatttta aaatgggaaa cattagttca agaggtgcta catgattaaa
    16441 ttatttgatt actttaacga tcattcacga aagttatatg agtcttttaa agccagtaag
    16501 ttggaagaag atttaacaat tgtactcaat gacaatggct ttttaccgga cgatgttata
    16561 tcaccgtatc aattttttgc agataatcat aatacagaaa atatgaaacc acgatttttt
    16621 aatcaggtta ctgtaccagc tttttgggaa attaaaggta acaacaattc ggctacaatt
    16681 aatgatatgg gacgtctacg aggtaaaata ttttatcaaa gtggagagcg ccctagaatt
    16741 gttagtcgtg tcgaatggtt tgatgaccaa caacgtgtcc gatttgtcga ttattattct
    16801 aagaatggta ttaaatttgc acaaactgtt tacgatttaa atcgtaaagc gatcttgaag
    16861 aaatatatga cagcagaagg taaagaagtg atttatgaaa attttgtaac atctgatgtc
    16921 atattggatt ggcaagggaa gtcttacttt ttcccttcaa agctcgcatt cgcattattt
    16981 tttatcaagc aacttgaaat tacggaacat catttcgtta ttaactcact agccctacca
    17041 ttttcagtgt tatataattt gccatcaaaa ggtagtgatg ttttggtatg gcaagaacac
    17101 tgtaatggta atgtcccggg aaatatgcaa ttgatgtgta aaggtgatat gaaacgacat
    17161 tgtaatatta ttattccaga taaaaacgaa tatgaaacga tgctcaatat agcagacgca
    17221 aaggttcaat ctcgtatttt acaaggcgga tatctatata actatcgtag tcgtaataga
    17281 tatactaaag aaattgtaat attaactaac tcggatcaac ttcgtaacat caaggtattg
    17341 gttgaaactt taccagattt taagtttcac atcgctgcta ttacagagat gtctgataag
    17401 ctaatgcaac ttgatcaata tgctaatgtg catttatacc catctattaa tattgacagg
    17461 gtaaatgaac tataccagtt atgtgatatt tatcttgata tcaatgaggg taatgaaatt
    17521 ttaaatgcgg tggaacaagc gtttgactat gagttgttga ttttaggtta tcgtcaaacc
    17581 gcacaccatg ctaaagtaac attatcagag cacttatttg agcataatga tgagataaca
    17641 atggaaagta aagaccaatt aatacaaatg ttagaaagtt tgaaagatca acaacagttt
    17701 cgagatgctt tattagcaca aaaagcacat gcacacgaaa tctcgcgtga acaattcgag
    17761 caagttttta aacaggcgct cgaaagctaa aaggaaaaag cagtctaaaa taaaacatca
    17821 tttaatataa aagatttgac gtaatatgat gttagcgaca tggacaatat gatattacgt
    17881 catgcttgtt ttgtttactt aaatataagc taagtaataa gtagataatt actaacaaca
    17941 ataactagat agataagtgt aaatttcttg taaacaggta tataatagta tgtaattctt
    18001 tgaaggaagt aagtgaggaa ggagtatgtt gatggctaaa tatcgaggga aaccgtttca
    18061 attatatgta aagttatcgt gttcgacaat gatggcgaca agtatcattt taacgaatat
    18121 cttgccgtac gatgcccaag ctgcatctga aaaggatact gaaattacaa aagagatatt
    18181 atctaagcaa gatttattag acaaagttga caaggcaatt cgtcaaattg agcaattaaa
    18241 acagttatcg gcttcatcta aagaacatta taaagcacaa ctaaatgaag cgaaaacagc
    18301 atcgcaaata gatgaaatca taaaacgagc taatgagttg gatagcaaag acaataaaag
    18361 ttctcacact gaaatgaacg gtcaaagtga tatagacagt aaattagatc aattgcttaa
    18421 agatttaaat gaggtttctt caaatgttga taggggtcaa caaagtggcg aggacgatct
    18481 taatgcaatg aaaaatgata tgtcacaaac ggctacaaca aaacatggag aaaaagatga
    18541 taaaaatgat gaagcaatgg taaataaggc gttagaagac ctagaccatt tgaatcagca
    18601 aatacacaaa tcgaaagatg catcgaaaga tacatcggaa gatccagcag tgtctacaac
    18661 agataataat catgaagtag ctaaaacgcc aaataatgat ggttctggac atgttgtgtt
    18721 aaataaattc ctttcaaatg aagagaatca aagccatagt aatcgactca ctgataaatt
    18781 acaaggaagc gataaaatta atcatgctat gattgaaaaa ttagctaaaa gtaatgcctc
    18841 aacgcaacat tacacatatc ataaactgaa tacgttacaa tctttagatc aacgtattgc
    18901 aaatacgcaa cttcctaaaa atcaaaaatc agacttaatg agcgaagtaa ataagacgaa
    18961 agagcgtata aaaagtcaac gaaatattat tttggaagaa cttgcacgta ctgatgataa
    19021 aaagtatgct acacaaagca ttttagaaag tatatttaat aaagacgagg cagttaaaat
    19081 tctaaaagat atacgtgttg atggtaaaac agatcaacaa attgcagatc aaattactcg
    19141 tcatattgat caattatctc tgacaacgag tgatgattta ttaacgtcat tgattgatca
    19201 atcacaagat aagtcgctat tgatttctca aattttacaa acgaaattag gaaaagctga
    19261 agcagataaa ttggctaaag attggacgaa taaaggatta tcaaatcgcc aaatcgttga
    19321 ccaattgaag aaacattttg catcaactgg cgacacgtct tcagatgata tattaaaagc
    19381 aattttgaat aatgccaaag ataaaaaaca agcaattgaa acgattttag caacacgtat
    19441 agaaagacaa aaggcaaaat tactggcaga tttaattact aaaatagaaa cagatcaaaa
    19501 taaaattttt aatttagtta aatcggcatt gaatggtaaa gcggatgatt tattgaattt
    19561 acaaaagaga ctcaatcaaa cgaaaaaaga tatagattat attttatcac caatagtaaa
    19621 tcgtccaagt ttactagatc gattgaataa aaatgggaaa acgacagatt taaataagtt
    19681 agcaaattta atgaatcaag gatcagattt attagacagt attccagata tacccacacc
    19741 aaagccagaa aagacgttaa cacttggtaa aggtaatgga ttgttaagtg gattattaaa
    19801 tgctgatggt aatgtatctt tgcctaaagc gggggaaacg ataaaagaac attggttgcc
    19861 gatatctgta attgttggtg caatgggtgt actaatgatt tggttatcac gacgcaataa
    19921 gttgaaaaat aaagcataat tatattgggg gaagagcatc tatatatttt tttaagtata
    19981 taagacgtct tatttcccct taatttattg tgaagtatat gcaaaatgca atgaatagat
    20041 tgtccatcat tttaacgtta taatgaattt aacgacttag aactacacaa gtaaaggaga
    20101 atgaagatgt ctcgaaaaac ggcgctatta gttttggata tgcaagaagg tatagcgagt
    20161 agtgtaccta gaataaaaaa tattattaaa gcgaatcaga gagcaattga agcagcaaga
    20221 caacatcgaa taccagtcat tttcatacgt ttagtgttag ataagcattt taatgatgtc
    20281 tcctcgagta ataaagtgtt ttcaacaatt aaagctcaag gatatgcgat tactgaagca
    20341 gatgcatcta cacgaatact tgaagattta gcaccactag aagatgagcc gattatttct
    20401 aagcgacgct ttagcgcatt tacaggtagt tacttggaag tttatttacg tgcaaatgat
    20461 attaatcatt tagtattaac gggtgtctct acaagtggag ctgtattgag cacggcatta
    20521 gaaagtgtag ataaagacta ttatattact gttttagaag atgctgttgg tgatagatca
    20581 gatgataaac atgactttat tattgaacaa attttatcac gctcatgtga cattgaatcc
    20641 gtagagtcat ggaaaagtag tttatagtta atataacgtc aattaaagct cggcagtaat
    20701 gtttgagaat aagtacattt gctcatattt ataaaatgtg tgagatggca attgaaacgg
    20761 atatgatgag gaacatttga acataaaata atatatttat ataaaacgac ccgaggcgtt
    20821 cgaactgaat gcctcgggtt taattgaata agaaatcaga cttatgaaca gaaatatgtt
    20881 taagtccgaa ctccttgttt atacttataa attttacggg tttaatataa tacttattta
    20941 cctgtaatat atgataattc ttcagcggca gctgcgttga tagttctatg agaaatgata
    21001 cctaatcctt taacattgga ttctgaaata acgatagaac catcactgtt aactttttca
    21061 acaaatgcta catgaccgta atgttgatct gcaccaaatt gtccagcctc aaatacaaca
    21121 gcagcatgac gttttggtgt atgacttact tgataatcac ggtattgagc tcgattattc
    21181 caattatgtg catcacctaa atcacctgag atagatgtac caaattgttt catacggtta
    21241 tatacgtacc aagtacattg gccatgtgga tatggcatac tatcagatac ctcacggaaa
    21301 ggtttgaatt catctgatga atcatcataa tccttgatag aacgttcata tttatctaaa
    21361 tctggcatgc gttcatcgtc aaactgagtt aattgatagt gtttaataat actgtttaat
    21421 ttcttagcat agtttggatc tgtagcatat gttttagata agtgtgatgt tgcatcttta
    21481 taagaatcgg cttccgattt ccatgttggt ttataaattg ttcgattgcc atcaatacca
    21541 tttttaataa ggtcagagta atcttttagt gattctttcg tgcttggata ttttcggaat
    21601 ccagcattaa tactatacaa ttgattacca tcagcttcta atgtgttaaa aggaacagaa
    21661 ttcccttcaa aagcaccttt gataccgaat aaattatggt ttggtgactt agctaaagca
    21721 ctacgacctg agtcagattc taagattgct tgggcaatca tgacagacgc ataaatatcg
    21781 ttatcttgac caatgcgatg tgcatcttta gcaattgatt tgacaaattg acgtgtatct
    21841 tttgagtcaa caacgttaaa ttgtccgcta tcatcattgt tagatatact aggatctgtt
    21901 tcgaataatg atgttgcacg tgtatccttt tgattaacat cgttattgaa tgattgagca
    21961 ggtttagatt tatgtttcaa ttcatcttgt gttggtaact gtggattctt tgtattagat
    22021 ttttcatttt tgtctttttt agattgagat gcataatctt tttgtgcttt ctttgcatct
    22081 tcactgtatt gatccaaaat agagtctaaa gccgaatctg acattgattg attatctttc
    22141 gatgaagatt tttgatttgc tttatcgtca cttgctggtt gactatttga ttgattaggt
    22201 tgtgttggct ttggcgaatt tggttgctta ttagatgtac ttggttttgt attgtttgat
    22261 ttaggtgctt tttgattgtc tgctttatct tgtttagatg attgcgtatc agtgtcattt
    22321 ttgatgctat tgtcactgtt tttattcgaa tcatttgttg acttttcgcc attacgaggt
    22381 tgttcgtaat cagaaatatc cgaatttaaa ttgaataagt tttggattaa agttgttaat
    22441 gagtaattat catcgtattt atttttggtt agcaattggt ttatattggt ttgtggtaaa
    22501 ttcttataaa taaaatcaat gatattgtta gagtctgaag tgctgtcgtc tatagtttta
    22561 aattttttgt cgttattgtc ttggttactt gtattatttt tgtctgcttt atcaatatct
    22621 ttacttgtag tatccttaga agtttcatcg tcattagatt tttttgaatc atgagatgtt
    22681 gtcttagctg tagtatcttt ttgaggtgta tcagcataag cggtaggtga aactaaagta
    22741 ggtaatacga gcgtagttga tagcaaataa attaaaattt tatttttagg catatttcgt
    22801 attctccctt gaaaaatata ataattaagt gtgataataa actatgattt gttataattt
    22861 atcgtatgct gaaaatagtt gataggtatc aatcgactaa atatcttcca gtaaattgat
    22921 tatactaatt cacaacgcaa aaataaatta atttacaaaa aatatataaa aaatatgaat
    22981 aattcctaca taggagtgtg acaatgaaga acgcatttaa attatttaaa atggatctga
    23041 agaaagtagc taagacgcca gctgtgtgga ttatcttagc aggcttagct attttgccat
    23101 cgttctacgc ttggtttaac ttatgggcaa tgtgggatcc atatggcaac acgggacaca
    23161 tcaaggtcgc agtcgttaat gaagataaag gcgacacaat cagagggaaa aaagttaatg
    23221 tcggtaatac gatggttaat acactcaaga aaaataaaag ttttgattgg cagtttgtaa
    23281 gtagagagaa agctgatcat gagataaaaa tgggtaaata ttttgcaggt atttacatcc
    23341 catctaagtt tacacatgaa attacaggga cactacgtaa gcagcctcaa aaagcagatg
    23401 tagaatttaa ggtgaatcag aagattaacg ctgttgcgtc taagctaaca gatactggtt
    23461 cgtcagttgt cgttgaaaaa gcgaatgaac aatttaataa aacagtaact cgagcattat
    23521 tagaagaagc taacaaagca ggtttaacta ttgaagaaaa tgtgccgaca attaacaaga
    23581 taaaaaatgc ggtatattca gcagataaag ctttacctaa gattaatgac tttgcgaata
    23641 aaattgtata tttgaataac caccaagcgg atttagataa atatgccaat gattttagaa
    23701 aactaggaaa ttataaaggt gatattttag atgctcagaa aaaattaaac gaagtcaatg
    23761 gtgctattcc gcaacttaat gaaaaggcta agttgatatt agctttaaat aattatatgc
    23821 cgaaaattga aaaagcgtta aattttgcag ctgatgacgt gccagcgcag ttccctaaaa
    23881 ttaatcaagg acttaacatt gcgagtcaag gtattgatca agctaatgga cagttaaatg
    23941 atgccaaagg cttcgtcaca caagttagaa gtagagtcgg tgattatcaa gatgcaattc
    24001 gacgcgcgca agatttaaat cgaagaaacc agcaacagat tcctcaaaat agcgcggcga
    24061 acaacgaaac atcaaatagt gcacctgcag ctggtaatgg tgtagcatca acgccaccaa
    24121 gtgcaccaag tggcgatact gcaccaaata ataatgttac gcaaaatacc gcaccaaata
    24181 gtaataatgc gcctgtatcg actacaccac aaagtacaag cgggaaaaaa gatggtcaaa
    24241 gttttgtaga tataacaaca acacaagtca gcacagctaa cgagaacaca caaaacatta
    24301 cagataaaga tgttaaatca atggaagcgg cattaacggg ctctttatta tcattatcaa
    24361 ataatttaga tacccaagcg aaagccgcac aaaaagatag tcaggcatta cgtaatattt
    24421 cgtatgggat tttagcatcg gacaagcctt ctgattttag agagtcttta gataatgtta
    24481 agtccggttt agaatacaca acgcaatata atcaacaatt tatcgataca ttaaaagaga
    24541 ttgagaagaa tgaaaatgtt gatttatcaa aagaaattga taaggtaaaa gcagctaata
    24601 atcgaattaa tgaatcatta aggttagtta atcaattaag caatgcatta aagaatggta
    24661 gttcaggaac tgctgaagct actaaattac tagatcaact ttcaaaacta gattcatcat
    24721 tatcatcatt tagagattat gttaaaaaag atcttaacag ctctttagta tcaatatcac
    24781 aacgtattat ggatgaattg aacaaagggc aaactgcatt atccaatgtt cagtctaaat
    24841 taaatacaat tgatcaagtc attaacagtg gacaagctat tttaaaaaat ggtaaaacac
    24901 gtattgatcg cttacaaaca gtattaccaa gtattgaaca acaatacatt agtgctgtta
    24961 aaaatgctca agcaaacttc tcgaaagtga aaagtgatgt agctaaagct gctaactttg
    25021 tgcgcaatga cttaccacag ttagaacagc gattaactaa tgcgacagca agtgtgaata
    25081 aaaatttacc aacgttatta aatggttatg atcaagcggt aggattacta aataaaaatc
    25141 agccacaagc gaaaaaggct ttatcagatt tagctgattt ttctcaaaat aaattgcctg
    25201 atgttgaaaa agatttgaaa aaagcgaata aaattttcaa gaaattagac aaagatgatg
    25261 cagtcgacaa attaatcgac acacttaaga atgatttgaa aaagcaagcg ggtattattg
    25321 caaatcctat taataagaag actgttgatg ttttcccagt taaggattat ggttcaggta
    25381 tgacaccatt ctatactgca ctgtcagtat gggtaggtgc actcttgatg gtaagtttat
    25441 taacggttga taataaacat aagagtctag agtcagtctt aacgacaaga caagtgttct
    25501 taggtaaggc aggattcttt ataatgcttg gtatgttgca agcactcatt gtatcggttg
    25561 gagatttgtt aatcctaaaa gcaggagttg agtcacctgt attatttgta cttataacga
    25621 ttttctgttc gattattttc aactcaatcg tatatacgtg cgtatcatta cttggtaacc
    25681 caggtaaagc cattgcaatc gtattgcttg tattacaaat tgcaggtggt gggggaacat
    25741 tcccaattca aactacgcca caatttttcc aaaacatttc gccatactta ccatttacgt
    25801 atgcaattga ttcattacgt gaaacagtag gcggtattgt tccggaaatc ctaattacaa
    25861 aattaattat attaacgtta tttggtatag gattcttcgt tgtaggttta attttaaaac
    25921 ctgtaacaga tccattgatg aagcgcgtat ctgaaaaagt tgaccaaagt aacgttacag
    25981 aataaaaatt aaatccacac attagggtta tagctcctta atgtgtggat ttttatgttt
    26041 ttagacagaa gagatagtaa tttctgtctt ttatgggacg gttgttatca ttgctattat
    26101 ccaggatgac ttactatagc actaatatta ccgacaaagt gaatatcctc gtcttccgta
    26161 gttaaaataa agctagaacc tttttggatg tcatagtgct tatcgtttac tgttaaagta
    26221 ccagtaccat cgataattgt aactaagcaa taagcatgtg gtttattgaa ttttaaatct
    26281 ccatgaatat cccatttata tactgcaaaa tattgattat ctacaaattg agttacagtg
    26341 tgtgtgtcga tgtgagttgt tataggagta gtatttggtt catgattgcc taattcaatc
    26401 acatctttac tttgctctaa gtgcaaatca cgcaattgac cattttgatc tcgtctatca
    26461 tagtcataaa tacggtatgt cgtatcggag gattgttgtg tctctaaaat taaaataccc
    26521 gaaccaatgg catggacagt gccagcagga acataataaa agtcaccggg cttaacaggt
    26581 atacgtttga aaagactgtc aaattcatga ttatcaatca tgtctattaa cgtctgttta
    26641 ttatgtgcat gtacgccata tataatttca gcacctgggg ctgcatctaa aatataccaa
    26701 cattctgttt tacctagttc gccttcgtgt tttaaagcgt agtcatcatc tgggtgaact
    26761 tgaacagata atttatcatt ggcatctaat actttagtta gcagagggaa actatctcgt
    26821 gaatcattat cgaataattc acgatgttgt gaccaaagtt gatctagggt catatccttg
    26881 tatggaccat tgataattgt attaggacca tttggatgtg cagaaattgc ccagcattca
    26941 ccagttgttt cattagggat atcatagtta aatgctttta atgcatgacc gccccaaatt
    27001 ctgtctttaa aaacgggttg taaaaataat gccatagtta aaactcctct atattttcat
    27061 taataagtta taaatttctg tagtactgtt tgcattaatt agtgattggc gtgtctcatc
    27121 attcattaac gctttagata agcgctgaag tatttttaaa tgtgtatcct gactgttgtt
    27181 tggtacggca attaagaata tcaattgagg tagactacca tctagactgt cccatttaac
    27241 accatgatta tttttcataa cagctacaat cggttgtttt acaacatcag actttgcatg
    27301 tggaatggcc acgttcatgc caatagctgt cgtagacccc atttcacgtt ctagtattgc
    27361 attttttaaa tgcgatgtgt gctctacata acggcaaatt ttaagtttat gaatcaacat
    27421 atcaattgct tcgtttcgag acatgtcgtg atcagtaatt atcatagttt gttgatcaaa
    27481 aacatgagaa ggtttattga gatgtgaatg tttcgcggtg ttatctacat tgtcaacctc
    27541 tgtatcatgt tgtgtaatat ctgtatcatg aagttgcgtg tgttgcgctg gtgcatctac
    27601 tgctataact ggtgtattgc gttttaataa tagtacagta gtcattgtga caagactacc
    27661 tactatcact gcaaagataa accataatac atgatcaata ccacctaata cagccacgat
    27721 tggacctcca tgtgcgactc tatcgccgac accaccaatg gctgcaatga ctgatgcaat
    27781 cattgcacca atgatgtttg caggtataat gcgcaatgga tcttgggctg cgaaaggaat
    27841 agcaccttca gtaataccaa atagtcccat agtgaaggaa gccttaccca tttctctttc
    27901 ggaatgattg aatttatact tttgaacaaa cgttgctaaa cctaaaccga ttggtggtgt
    27961 acatacagca actgcgacca tacccataac ggcgtaatta ccttcagcaa taagtgctga
    28021 gccaaataaa aatgctacct tgtttactgg accgcccata tcgaaggcaa tcatcgcacc
    28081 tataatcatc gcaagtataa taatattagc accttgcata ctttttaacc aggttgttaa
    28141 tgcctcaaaa atattagaaa ttggtgcacc gattaaaaat ataaatatca atcctacaac
    28201 gaccgatgaa ataatgggaa taataatgat aggcataatt ggtgccattg cttttggaac
    28261 tttaatatct ttaatccact ttgcgatata acctgctaag aaaccagcaa caataccacc
    28321 taaaaatcct gcgcctgcat cactgccata aaaactaccg tcagcagcga tagcgccgcc
    28381 aatcatacca ggaacaagac cgggcttgtc agcgatacta acagcgatat atccagctag
    28441 tattggaacc ataaatttaa aggctaaact accaatgttt tcaatggatt tccaaaatga
    28501 atcatctggg atgactaatc cttttgatgt cgtttcaccg cctagagtca gcgcgatggc
    28561 gataaggagt ccaccaacta cgataaaagg aaccataaac gatacaccgt tcattaaatg
    28621 ttgatacacc atttgaatac cacttttaga cttaccgcga tctttcgaat gataatttgt
    28681 ttcagattga taaataggcg catcttgatt aatgatacgt tgaattagac ctctcggatt
    28741 atgaatccct tcgcgaacat tttcattaat caaccgttta ccaacaaatc gggacagatc
    28801 aacttgttta tcagctgcaa ttatgacacc gtcagcttct tcgatgtctt gcgtagttaa
    28861 aacattttca gcaccaacac cgccctgtgt ctctacttta atatccacac ccatttcttt
    28921 tgctacctgc tcaagctttt cttgagccat atatgtatgt gcaatgccat ttgggcatga
    28981 ggtaatagct acaattttca taaaatcatc tccttttcta tattgtaagc gtattctcga
    29041 tactaaaaaa aagaataatt accgttacta gtggcaatta ttcttgtaag tattcaaata
    29101 actgttgctt taaactatga tcatctaaac tacataaatg gttcactgaa tcatcatcca
    29161 agttagcaat taattgcatc atttgttttg taaaagcttt gtctttatgc gaaatcgcta
    29221 agaaaaagac aagtttgaca tcgtgttgtc gccaaggaaa aacatctttt gtgcgaaaaa
    29281 taagcacatg tgattgtaaa actttttcag gatctccatg aggaatcgcc ataaaattac
    29341 ctatgtatgt agaagatgat ttctcacgct ctaaagctga ttcgatatat ccttctacaa
    29401 tcgcatgatg tgcttgtaat attttttgag cttcttcaaa aatttgcaca gtatgccgtg
    29461 atttttgttc agtatttacg acaaggaaat tgacagtgtc catatgatga tgtgcttgaa
    29521 ccggattttg cttttgcttc acaacgtgtc tgattttgtg acgatcatct tcagaaaata
    29581 atggtgcaac cttgatagtc gtcaggtgct taggaagtat gtttagcgtt tgtttaggaa
    29641 tatcatgggt cgttattaat aaatctacat tgtcaaagtg atagtgtgtt atattttcta
    29701 gtttaatcgt atttatcact gacaactctt cggataagtt atttatttta gtttctaaaa
    29761 aattcgacac acctagacca taataacaag caatgactac atttaattgt gttttggtac
    29821 gacgctcgat ggcagcttga aaatgaattg ttaaaaatgc aatttcatct tcgctcatct
    29881 ctatatcagt atcaattgct aatttatcaa tcgcttcaaa aagtgtgtta aacacaaagg
    29941 gatagagttt tttaatctct ataactaaag gattgtttaa ataaatgttt tgagtgatac
    30001 gtaaatatgc tttactaaaa tgattatata aattttgttg taaaatcgaa tcttcattga
    30061 aaggtacatg aatacgttgc tgcatcaatt cgattaagcg atcaatataa ctttgtataa
    30121 atatacgttc tatgccaata tcgagtttat taaaatgata agcaataaag aatgaaaaca
    30181 tattgattac tttttcgttc aagtcataac ctaatctttc gttgatttgc ttaatgcaag
    30241 attgagatat caattttgaa tcgtggttta acgtgtctag ctcacttaaa gattgcttac
    30301 gtttaatgat taaaatgaga tgaattaaaa gctgttgtat ttgaatatca gttgtttcaa
    30361 tactatgttg ttgaagtgtc tcttgtataa tatgcgaaat catcctttgg tgtgaatcag
    30421 gtaattcatt taaaattagg tcttcaacat gtacatgccc tgatgataat tgatttaaat
    30481 ggatgatggc attagtgata tcattatctg ttccatcgac cacaatacct tgttttttct
    30541 tgattgttaa agcgatatta aatttttgac accattgttg aattcgaacg aaatagtcgt
    30601 tgagcacagt tttagataat agataatctt gctctagttg ttgcaatgtc tgcgatttat
    30661 tgtgcattag cagttgatac gccatagata ataatagttt ttctttatat gattgaatat
    30721 gcgtgtaacg ttcagttatc gtttctaagg tatactgaga tgtatttaat tgatatcctt
    30781 tagatttaat acttacgatg atatcatcca taaaattgct attgataaca tggatatcat
    30841 tgcgaactgt gcggtttgaa acattgacat gtttagcaat ttcattagaa ctaatgtgct
    30901 ttgatggatt tttaataaaa aactggagta gttttaagtg tctatcaagc atttaacatg
    30961 tacctccttt ctaaattttt cgtgtaagcg tttttaaggg tgtattaata ttattaaaca
    31021 tgagagctta tacatacgtc aatgacatta aagcgaactt ttatatgatt ttaacagagt
    31081 gcgaattatg caaataaaga acagcagtaa gatatttcaa atagaaaaat atctcactgc
    31141 tgtttttctg aatttatgca tctggtactt gtggacgtat caggcaaatg attaattttt
    31201 aggtgattgt gcttgaggtg tttgtttaga aggcgtattg gtattatttg atttattagc
    31261 aggtggtgtg ttctgttgtt gattattttg gttgtttgtt gacgattgac tgccaccacc
    31321 gtgagtatta tttttatttg aatttgagtc ttttggctga gctggttgcg tattcgatgg
    31381 tactgttgaa tggttgttag gaacatttga cggtgaagat ggttgattgt cattagtaac
    31441 gttattgcca ttattttgat ttttatctgt tttgtctgca ctatcatctt gttgatcatt
    31501 ttcttcggtt tctgtctttt tatgcgtaga tttattttct tgttcttggt tattacttga
    31561 tgatgtagaa tgatttattg cagcataaat tgcgaaccca agtgctccga ttaatacgat
    31621 aattacgatg gtaataagaa gttttttcaa atataacgcc tccatgttaa tgaaaagtag
    31681 ttgatacgtg aagctaaata atttatttca atattgccat aatcatttta aaaaataaat
    31741 attagaaaag taggcgatat taatattttc attgtttttt gtgtacttca attagtataa
    31801 caaaactgtt atgtatttat aaatataatc attagtattt ttatggctga aaaagttata
    31861 ataaaagtgt aaggaataaa atatttgtat ggaaaagaga gataattatg aataaaacca
    31921 gtaaagtttg tgtagcagca acattagcat tgggcacact gattggagta acagttgttg
    31981 aaaattcggc gccaacaagt aaacaggcac aggcagcaat aaccccatat tatacttata
    32041 atggttatat tggtaataat gctaatttta ttctggataa gaattttatt aacgcgatta
    32101 agtatgataa tgtgaaattc aatggtatta aattagctaa aacgaatacg ataaaaaaag
    32161 tagagaaata tgatcaaact tttaaaggtg tttctgcaaa aggaaacgaa gcaagtcaat
    32221 tgcaatttgt agttaaaaat aatatttcat taaaagatat ccaaaaagct tatggcaagg
    32281 acttgaaaaa agaaaatggt aaaacaaagg aagctgatag cggtattttt tactatcaaa
    32341 atgctaaaaa gacattaggc atctggtttg tcgttgatca taatagagtt gtcgaagtaa
    32401 cagttggaca tacaccatac aaaacaagta aataaaataa tggcatatta aggctagagt
    32461 gtgaggagtg ataccgcatt ctagtctttt ttattaaata ataacgatta ttgcgtctta
    32521 catagttgtt tgaaattgat aaaaatgttt atttgattat gattatttaa atagttatct
    32581 cacatatttc
//
LOCUS       DAKTIP010000009        30555 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAKTIP010000009 DAKTIP010000000
VERSION     DAKTIP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 30555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.009"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            16..633
                     /locus_tag="O0305_000311"
     CDS             16..633
                     /locus_tag="O0305_000311"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCY0273497.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            661..990
                     /locus_tag="O0305_000312"
     CDS             661..990
                     /locus_tag="O0305_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCY0273498.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            1204..2130
                     /locus_tag="O0305_000313"
     CDS             1204..2130
                     /locus_tag="O0305_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCY0273499.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            2131..2934
                     /locus_tag="O0305_000314"
     CDS             2131..2934
                     /locus_tag="O0305_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCY0273500.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIEDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRIADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            2951..3298
                     /gene="yabA"
                     /locus_tag="O0305_000315"
     CDS             2951..3298
                     /gene="yabA"
                     /locus_tag="O0305_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCY0273501.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            3572..4297
                     /locus_tag="O0305_000316"
     CDS             3572..4297
                     /locus_tag="O0305_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCY0273502.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            4290..4538
                     /locus_tag="O0305_000317"
     CDS             4290..4538
                     /locus_tag="O0305_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCY0273503.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            4540..5379
                     /gene="rsmI"
                     /locus_tag="O0305_000318"
     CDS             4540..5379
                     /gene="rsmI"
                     /locus_tag="O0305_000318"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCY0273504.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            5664..7637
                     /gene="metG"
                     /locus_tag="O0305_000319"
     CDS             5664..7637
                     /gene="metG"
                     /locus_tag="O0305_000319"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCY0273505.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            7668..8441
                     /locus_tag="O0305_000320"
     CDS             7668..8441
                     /locus_tag="O0305_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCY0273506.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            8608..9144
                     /gene="rnmV"
                     /locus_tag="O0305_000321"
     CDS             8608..9144
                     /gene="rnmV"
                     /locus_tag="O0305_000321"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCY0273507.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHAELIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            9155..10048
                     /gene="rsmA"
                     /locus_tag="O0305_000322"
     CDS             9155..10048
                     /gene="rsmA"
                     /locus_tag="O0305_000322"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCY0273508.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            10148..10411
                     /locus_tag="O0305_000323"
     CDS             10148..10411
                     /locus_tag="O0305_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCY0273509.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            10721..11569
                     /locus_tag="O0305_000324"
     CDS             10721..11569
                     /locus_tag="O0305_000324"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCY0273510.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            11583..12407
                     /gene="purR"
                     /locus_tag="O0305_000325"
     CDS             11583..12407
                     /gene="purR"
                     /locus_tag="O0305_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCY0273511.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            12424..12804
                     /locus_tag="O0305_000326"
     CDS             12424..12804
                     /locus_tag="O0305_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCY0273512.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            12876..13178
                     /gene="spoVG"
                     /locus_tag="O0305_000327"
     CDS             12876..13178
                     /gene="spoVG"
                     /locus_tag="O0305_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCY0273513.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            13368..13499
                     /locus_tag="O0305_000328"
     CDS             13368..13499
                     /locus_tag="O0305_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273514.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            13522..14874
                     /gene="glmU"
                     /locus_tag="O0305_000329"
     CDS             13522..14874
                     /gene="glmU"
                     /locus_tag="O0305_000329"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCY0273515.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            15021..15986
                     /locus_tag="O0305_000330"
     CDS             15021..15986
                     /locus_tag="O0305_000330"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCY0273516.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            16136..16789
                     /locus_tag="O0305_000331"
     CDS             16136..16789
                     /locus_tag="O0305_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCY0273517.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            17100..17672
                     /locus_tag="O0305_000332"
     CDS             17100..17672
                     /locus_tag="O0305_000332"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCY0273518.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            17672..21178
                     /gene="mfd"
                     /locus_tag="O0305_000333"
     CDS             17672..21178
                     /gene="mfd"
                     /locus_tag="O0305_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCY0273519.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            21168..22694
                     /locus_tag="O0305_000334"
     CDS             21168..22694
                     /locus_tag="O0305_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0273520.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILLFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLYAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            22694..23887
                     /locus_tag="O0305_000335"
     CDS             22694..23887
                     /locus_tag="O0305_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0273521.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHNQFEDVYIDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVIFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            23884..24147
                     /locus_tag="O0305_000336"
     CDS             23884..24147
                     /locus_tag="O0305_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCY0273522.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            24165..24557
                     /locus_tag="O0305_000337"
     CDS             24165..24557
                     /locus_tag="O0305_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCY0273523.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            24662..25063
                     /locus_tag="O0305_000338"
     CDS             24662..25063
                     /locus_tag="O0305_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCY0273524.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            25243..26538
                     /gene="tilS"
                     /locus_tag="O0305_000339"
     CDS             25243..26538
                     /gene="tilS"
                     /locus_tag="O0305_000339"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCY0273525.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            26543..27082
                     /gene="hpt"
                     /locus_tag="O0305_000340"
     CDS             26543..27082
                     /gene="hpt"
                     /locus_tag="O0305_000340"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCY0273526.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            27339..29432
                     /gene="ftsH"
                     /locus_tag="O0305_000341"
     CDS             27339..29432
                     /gene="ftsH"
                     /locus_tag="O0305_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCY0273527.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHQQA
                     PNIEKPYDPNHPDNK"
     gene            29661..30542
                     /gene="hslO"
                     /locus_tag="O0305_000342"
     CDS             29661..30542
                     /gene="hslO"
                     /locus_tag="O0305_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCY0273528.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
BASE COUNT        11068 a         4098 c         6139 g         9250 t
ORIGIN      
        1 ttgaggatga ataaaatgtc agcttttata acttttgagg gcccagaagg ctctggaaaa
       61 acaactgtaa ttaatgaagt ttaccataga ttagtaaaag attatgatgt cattatgact
      121 agagaaccag gtggtgttcc tactggtgaa gaaatacgta aaattgtatt agaaggcaat
      181 gatatggaca ttagaactga agcaatgtta tttgctgcat ctagaagaga acatcttgta
      241 ttaaaggtca taccagcttt aaaagaaggt aaggttgtgt tgtgtgatcg ctatatcgat
      301 agttcattag cttatcaagg ttatgctaga gggattggcg ttgaagaagt aagagcatta
      361 aacgaatttg caataaatgg attatatcca gacttgacga tttatttaaa tgttagtgct
      421 gaagtaggtc gcgaacgtat tattaaaaat tcaagagatc aaaatagatt agatcaagaa
      481 gatttaaagt ttcacgaaaa agtaattgaa ggttaccaag aaatcattca taatgaatca
      541 caacggttca aaagcgttaa tgcagatcaa cctcttgaaa atgttgttga agacacgtat
      601 caaactatca tcaaatattt agaaaagata tgatataatt gttagaagag gtgttataaa
      661 atgaaaatga ttatagcgat cgtacaagat caagatagtc aggaacttgc agatcaactt
      721 gttaaaaata actttagagc aacaaaattg gcaacaacag gtgggttttt aagagcgggt
      781 aatacaacat tcttatgtgg tgtcaatgat gaccgtgtag atgaaatatt gtctgtgatt
      841 aatcaaacgt gtggtaatag agaacagttg gtttcaccta ttacacctat gggaggcagt
      901 gcggattcgt acattccata tccagttgaa gttgaagttg gcggtgctac tgtatttgtt
      961 atgccagttg atgcattcca tcaattttaa ttctataata caatcatcaa ttagagatac
     1021 ttaaaatagt gtattaataa gttattaaca attttgggtt gcttgactgc gactagttca
     1081 gatgccaata gatttgattt ttgtggttct aaaaataatc acaaatcatg gtcgctattg
     1141 ttgcagtaat tagttgctct ttggcaacct ttttatataa aagcaaaagg gagtttgtaa
     1201 tgaatggatg aacagcaaca attgacgaat gcatatcatt caaataaatt atcgcatgcc
     1261 tatttatttg aaggtgatga tgcacaaacg atgaaacaag ttgcgattaa ttttgcaaag
     1321 cttattttat gtcaaacaga tagtcaatgt gaaacaaagg ttagtacata taatcatcca
     1381 gactttatgt atatatcaac aactgagaat gcaattaaga aagaacaagt tgaacaactt
     1441 gtgcgtcata tgaatcaact tcctatagaa agcacaaata aagtgtacat cattgaagac
     1501 tttgaaaagt taactgttca aggggaaaac agtatcttga aatttcttga agaaccaccg
     1561 gacaatacga ttgctatttt attgtctaca aaacctgagc aaattttaga cacaatccat
     1621 tcaagatgtc agcatgtata tttcaagcct attgataaag aaaagtttat aaatagatta
     1681 gttgaacaaa acatgtctaa gccagtagct gaaatgatta gtacttatac tacgcaaata
     1741 gataatgcaa tggctttaaa tgaagaattt gatttattag cattaaggaa atcagttata
     1801 cgttggtgtg aattgttgct tactaataag ccaatggcac ttataggtat tattgattta
     1861 ttgaaacagg ctaaaaataa aaaactgcaa tctttaacta ttgcagctgt gaatggtttc
     1921 ttcgaagata tcatacatac aaaggtaaat gtagaggata aacaaatata tagtgattta
     1981 aaaaatgata ttgatcaata tgcgcaaaag ttgtcgttta atcaattaat tttgatgttt
     2041 gatcaactga cggaagcaca taagaaattg aatcaaaatg taaatccaac gcttgtattt
     2101 gaacaaatcg taattaaggg tgtgagttag atgccaaatg taataggtgt tcagtttcaa
     2161 aaagcgggaa aattagaata ttatacacct aatgatatac aagtagatat agaagactgg
     2221 gtagttgtcg aatctaaaag aggcatagag ataggtattg ttaaaaatcc attaatggat
     2281 attgctgaag aggatgttgt gttacctctt aaaaatatta ttcgcattgc tgatgacaaa
     2341 gatattgata aatttaattg taatgaacga gatgctgaaa atgcattaat actatgtaaa
     2401 gacattgtaa gagaacaagg tttggacatg cgtttagtca attgcgaata tacattagat
     2461 aaatcgaaag ttatttttaa ttttacggcg gatgatcgta ttgattttag aaaattagta
     2521 aaaatattag cgcaacattt aaaaacacgt atcgagttga gacaaattgg tgtaagggat
     2581 gaagccaaat tgcttggcgg tatcggacct tgtggtaggt cgttatgttg ttctacattt
     2641 ttaggggatt ttgaaccagt atcgattaag atggctaagg atcaaaattt atcattaaat
     2701 ccaactaaaa tttctggtgc atgtggtcgt ttgatgtgtt gtttaaaata tgaaaatgac
     2761 tattatgagg aagtacgtgc acaattacct gatattggtg aagcaattga aacgcctgat
     2821 ggtaacggga aagtagttgc tttaaatata ttagacattt ctatgcaggt gaagcttgag
     2881 ggacatgaac agccacttga atataaatta gaagaaatag aaactatgca ttaaggaggc
     2941 attattacat ttggatcgca atgaaatatt tgaaaaaata atgcgtttag aaatgaatgt
     3001 caatcaactt tcaaaggaaa cttcagaatt aaaggcactt gcagttgaat tagtagaaga
     3061 aaatgtagcg cttcaacttg aaaatgataa tttgaaaaag gtgttgggca atgatgaacc
     3121 aactactatt gatactgcga attcaaaacc agcaaaagct gtgaaaaagc cattaccaag
     3181 taaagataat ttggctatat tgtatggaga aggatttcat atttgtaaag gcgaattatt
     3241 tggaaaacat cgacatggtg aagattgtct gttctgttta gaagttttaa gtgattaatc
     3301 aagcacactc aaatagtgtt ataattataa atgaatatgg tttggataag tctgagacaa
     3361 tgcatgtttc aggctttaat tgtgtataaa gttttggtga ttgcataaga gatggcggta
     3421 ctaaatgtta ttattaagtg tgcacgcagt atcattagtt ataaaatgta gctgttaaaa
     3481 gtcaaaaata catcgaatgt agttaggcat ataatataaa aagagttttc aattactcaa
     3541 tagaaaaagg ttgtcttcat aggagttaaa aatgttaaaa gagaatgaac gatttgatca
     3601 actaatcaaa gaagatttta gtattattca aaatgatgat gttttttcat tttcaacgga
     3661 tgctttgttg ttagggcatt ttacaaaacc tagaacaaaa gatattgtgt tggacttatg
     3721 ttcaggcaat ggggtgatac ccttgttatt gtttgcgaaa catccacgac atatagaagg
     3781 tgttgagatt caaaaaacac ttgtcgatat ggcgcgacgc acatttcaat tcaatgatgt
     3841 tgatgaatat ttaacaatgc atcacatgga tttgaaaaac gttactaaag tatttaaacc
     3901 ttcacaatat actttagtaa cgtgtaatcc gccttatttt aaagagaatc agcaacacca
     3961 acatcaaaaa gaagcacata agatagcgag acatgagatt atgtgtacac ttgaagattg
     4021 catgattgca gcccgtcatt tattaaaaga aggtggcagg ctaaacatgg tacatcgtgc
     4081 agagagacta atggatgtct tgtttgaaat gagaaaagtg aatattgaac ctaagaaagt
     4141 cgtttttata tatagtaaag tagggaaatc agcacaaacg atagtagtag aaggtcgaaa
     4201 aggtggaaat caaggtttag aaatcatgcc cccattttat atttataatg aagatggtaa
     4261 ttatagcgaa gaaatgaagg aagtatatta tggatagtca ttttgtatat attgtaaaat
     4321 gtagtgatgg aagtttatat acaggatacg ctaaagacgt taatgcacgt gttgaaaaac
     4381 ataaccgagg tcaaggagcc aaatatacga aagtaagacg tccggtgcat ttagtttatc
     4441 aagaaatgta tgagacaaag tctgaagcat tgaagcgtga atatgaaatt aaaacttata
     4501 ccagacaaaa gaaattgcga ttaattaagg agcgatagta tggctgtatt atatttagtg
     4561 ggcacaccaa ttggtaattt agcagatatt acttatagag cagttgatgt attgaaacgt
     4621 gttgatatga ttgcttgtga agacactaga gtaactagta aactgtgtaa tcattatgat
     4681 attccaactc cattaaagtc atatcacgaa cataacaagg ataagcagac tgcttttatc
     4741 attgaacagt tagaattagg tcttgacgtt gcgctcgtat ctgatgctgg attgccctta
     4801 attagtgatc ctggatacga attagtagtg gcagccagag aagctaatat taaagtagag
     4861 accgtgcctg gacctaatgc tgggctgacg gctttgatgg ctagtggatt accttcatat
     4921 gtatatacat ttttaggatt tttgccacga aaagagaaag aaaaaagtgc tgtattagag
     4981 caacgtatgc atgaaaatag cacattaatt atatacgaat caccgcatcg tgtgacagat
     5041 acattaaaaa caattgcaaa gatagatgca acacgacaag tatcactagg gcgtgaatta
     5101 actaagaagt tcgaacaaat tgtaactgat gatgtaacac aattacaagc attgattcag
     5161 caaggcgatg taccattgaa aggcgaattc gttatcttaa ttgaaggtgc taaagcgaac
     5221 aatgagatat cgtggtttga tgatttatct atcaatgagc atgttgatca ttatattcaa
     5281 acttcacaga tgaaaccaaa acaagctatt aaaaaagttg ctgaagaacg acaacttaaa
     5341 acgaatgaag tatataatat ttatcatcaa ataagttaat cactttatcg attatatgaa
     5401 attttaaacg attttataaa cgcaagctgt aattttaaat ggtaagttat cattttgcat
     5461 tgatactgat aaaatgatgt tgactatgat aaaaaaatga tgacatcgac gttttttaat
     5521 gtaaaataaa tacattgaaa gtaataaata ccttaacatt gaataagatg aaaatgagat
     5581 gacgagataa atgttcgcgt ccgttgaaat gcatagaaat cttagatatt atttgaagtg
     5641 agacattacg aggaggaaca gttatggcta aagaaacatt ttatataaca accccaatat
     5701 actatcctag tgggaattta catataggac atgcatattc tacagtggct ggagatgtta
     5761 ttgcaagata taagagaatg caaggatatg atgttcgcta tttgactgga acggatgaac
     5821 acggtcaaaa aattcaagaa aaagctcaaa aatctggtaa gacagaaatt gaatatttgg
     5881 atgagatgat tgctggaatt aaacaattgt gggctaagct tgaaatttca aatgatgatt
     5941 ttatcagaac aactgaagaa cgtcataaac atgtcgttga gcaagtgttt gaacgtttat
     6001 taaagcaagg tgatatctat ttaggtgaat atgaaggttg gtattctgtt ccggatgaaa
     6061 catactatac agagtcacaa ttagtagacc cacaatacga aaacggtaaa attattggtg
     6121 gcaaaagtcc agattctgga cacgaagttg aactagttaa agaagaaagt tatttcttta
     6181 atattagtaa atatacagac cgtttattag agttctatga ccaaaatcca gattttatac
     6241 aaccaccatc aagaaaaaat gaaatgatta acaacttcat taaaccagga cttgctgatt
     6301 tagctgtttc tcgtacatca tttaactggg gtgtccatgt tccgtctaat ccaaaacatg
     6361 ttgtttatgt ttggattgat gcgttagtta actatatttc agcattaggc tatttatcag
     6421 atgatgagtc actatttaac aaatactggc cagcagatat tcatttaatg gctaaggaaa
     6481 ttgtgcgatt ccactcaatt atttggccta ttttattgat ggcattagac ttaccgttac
     6541 ctaaaaaagt ctttgcacat ggttggattt tgatgaaaga tggaaaaatg agtaaatcta
     6601 aaggtaatgt cgtagaccct aatattttaa ttgatcgcta tggtttagat gctacacgtt
     6661 attatctaat gcgtgaatta ccatttggtt cagatggcgt atttacacct gaagcatttg
     6721 ttgagcgtac aaatttcgat ctagcaaatg acttaggtaa cttagtaaac cgtacgattt
     6781 ctatggttaa taagtacttt gatggcgaat taccagcgta tcaaggtcca cttcatgaat
     6841 tagatgaaga aatggaagct atggctttag aaacagtgaa aagctacact gaaagcatgg
     6901 aaagtttgca attttctgtg gcattatcta cggtatggaa gtttattagt agaacgaata
     6961 agtatattga cgaaacaacg ccttgggtat tagctaagga cgatagccaa aaagatatgt
     7021 taggcaatgt aatggctcac ttagttgaaa atattcgtta tgcagctgta ttattacgtc
     7081 cattcttaac acatgcgccg aaagagattt ttgaacaatt gaacattaac aatcctcaat
     7141 ttatggaatt tagtagttta gagcaatatg gtgtgcttaa tgagtcaatt atggttactg
     7201 ggcaacctaa acctattttc ccaagattgg atagcgaagc ggaaattgca tatatcaaag
     7261 aatcaatgca accgcctgct actaaagagg aaaaagaaga gattcctagc aaacctcaaa
     7321 ttgatattaa agactttgat aaagttgaaa ttaaggcagc aacgattatt gatgctgaac
     7381 atgttaagaa gtcagataag cttttaaaaa ttcaagtaga cttagattct gaacaaagac
     7441 aaattgtatc aggaattgcc aaattctata caccagatga tattattggt aaaaaagtag
     7501 cagttgttac taacctgaaa ccagctaaat taatgggaca aaaatctgaa ggtatgatat
     7561 tatctgctga aaaagatggt gtattaacct tagtaagttt accaagtgca attccaaatg
     7621 gtgcagtgat taaataactg tatttttaaa aattaggaga gataattatg ttaatcgata
     7681 cacatgtcca tttaaatgat gagcaatacg atgatgattt gagtgaagtg attacacgtg
     7741 ctagagaagc aggtgttgat cgtatgtttg tagttggttt taacaaatcg acaattgaac
     7801 gcgcgatgaa attaatcgat gagtatgatt ttttatatgg cattatcggt tggcatccag
     7861 ttgacgcaat tgattttaca gaagaacact tggaatggat tgaatcttta gctcagcatc
     7921 caaaagtgat tggtattggt gaaatgggat tagattatca ctgggataaa tctcctgcag
     7981 atgttcaaaa ggaagttttt agaaagcaaa ttgctttagc taagcgtttg aagttaccaa
     8041 ttatcattca taaccgtgaa gcaactcaag actgtatcga tatcttattg gaggagcatg
     8101 ctgaagaggt aggcgggatt atgcatagct ttagtggttc tccagaaatt gcagatattg
     8161 taactaataa gctgaatttt tatatttcat taggtggacc tgtgacattt aaaaatgcta
     8221 aacagcctaa agaagttgct aagcatgtgt caatggagcg tttgctagtt gaaaccgatg
     8281 caccgtatct ttcgccacat ccgtatagag ggaagcgaaa tgaaccggcg agagtaactt
     8341 tagtagctga acaaattgct gaattaaaag gcttatctta tgaagaagtg tgcgaacaaa
     8401 caactaaaaa tgcagagaaa ttgtttaatt taaattcata aagttaaaag tgagaaagat
     8461 caccgccata aatgtaaacg atgctatatt cgtttaatat gctatggttc tttctcactt
     8521 ttttaaatta aaatatcgtg catgtggaat acgtgcgata gagatggtta gagctttgaa
     8581 attaagaatt gtaggaaggc gttttaaatg aaaatcaatg agtttatagt tgtagaagga
     8641 cgagatgata ctgagcgtgt taaacgagct gttgaatgtg atacgattga aacgaatggt
     8701 agtgccatca acgaacaaac tttagaagta attagaaatg ctcaacaaag tcgaggcgtt
     8761 attgtattaa cagatccaga tttcccagga gataaaatta gaagtacaat tactgaacat
     8821 gtcaaaggtg ttaaacatgc gtatattgat agagaaaaag ctaaaaataa aaaagggaaa
     8881 attggtgttg aacatgccga attaattgat attaaagaag cgttaatgca tgttagttca
     8941 ccctttgatg aagcttatga atcaattgat aaatctgtgc taatagagtt ggggttaatt
     9001 gttgggaaag atgcaaggcg ccgtagagaa attttaagta gaaaattgcg aatcggccat
     9061 tccaatggta agcagttatt gaaaaagtta aatgcatttg gttataccga agcggatgta
     9121 aggcaagctt tagaagatga atgaggaagt gaaaatgttg gataataaag atattgcaac
     9181 accatcaaga acgcgagcgt tgttagataa atatggcttt aattttaaaa aaagtttagg
     9241 acagaacttt ttgatagatg tgaatatcat taataatatc attgatgcaa gtgatattga
     9301 tgcacaaact ggggtgattg aaattggtcc aggcatgggg tcattgacag aacaattggc
     9361 cagacatgct aaaagagtat tggcatttga aattgatcaa cgtttaatac ctgtattaaa
     9421 tgatacacta tcaccttatg ataatgtgac ggtgattaat gaagatattt taaaagcgaa
     9481 tattaaagaa gctgttgaaa atcatttaca agattgtgaa aaaataatgg ttgttgcaaa
     9541 cctgccgtac tatattacga cgccaatttt attaaatttg atgcaacaag atataccaat
     9601 tgatggctac gtggtgatga tgcaaaaaga agtgggcgaa cgcttaaatg ctgaagtagg
     9661 ttcaaaagca tatggttcgt tatcaattgt cgtacaatac tatacagaga ctagtaaagt
     9721 attaacggta cctaaatctg tatttatgcc accacctaat gttgattcaa tagttgtaaa
     9781 actgatgcag agaactgaac cgttagtaac agtagataac gaggaagcat tctttaagtt
     9841 agcaaaagca gcatttgcac aaagaagaaa gacaattaac aataactatc aaaattattt
     9901 taaagatggt aaacaacaca aagaagtgat tttacaatgg ttggaacaag caggtattga
     9961 tccaagacgt cgcggtgaaa cgctatctat tcaagatttt gctaaattgt atgaagaaaa
    10021 gaaaaaattc cctcaattag aaaattaaat gattgacaaa gcaaagcact attgttaaaa
    10081 tttaaatttt gtttgacgaa aacgttgcaa atatggtatt atgtaacttg tagcgaggtg
    10141 gagcaatatg ccaaaatcaa ttttggacat caaaaattct attgattgtc atgtaggaaa
    10201 tcgtattgta ctgaaagcca atggaggccg taagaaaaca ataaaacgtt ctggaatttt
    10261 aaaagaaaca tatccgtcag ttttcattgt tgagttagat caagacaaac acaactttga
    10321 gagagtatct tatacataca ctgatgtgtt aactgaaaat gttcaagttt catttgaaga
    10381 ggataatcat cacgaatcaa ttgcacacta aataagacat atagagatgt tagacgtttc
    10441 ttagtataag aagtaaatat tatgataatt atttgagtgt tgggcattat gttcaatact
    10501 ctttttattt acaaaatgtt taacactgat gtttcgctta tagatttttc agtaaatgga
    10561 taattgtatt tataaacaca aatacaagta aatactaagt aattagatgg agaaaattac
    10621 ttttttatta aaaaaacact aaaaaacaaa ttaaaatgtc aaatattaat tctctttatg
    10681 ttaaaatcat catattaaga taacgaaaag agggcggaaa atgatatatg aaacggcacc
    10741 agccaaaatt aattttacgc tcgatacact ttttaaaaga aatgatggct atcatgagat
    10801 tgaaatgata atgacaacag ttgatttaaa tgatcgttta acttttcata aaagaaaaga
    10861 tcgaaagata gttgttgaga ttgaacataa ttatgtgcct tctaatcata aaaatctcgc
    10921 atatcgtgca gcgcaactat ttattgagca atatcaacta aagcaaggtg taacaatttc
    10981 tatcgataaa gaaatacctg tttctgctgg cttagctgga ggttcggctg atgcagcagc
    11041 aacgttaaga ggattgaatc gactttttga tataggggcg agtttggaag aattggctct
    11101 actaggcagt aaaatcggga cagatattcc gttttgtatt tataataaaa ctgcactatg
    11161 tactggaaga ggagagaaaa tcgagttttt aaataaacca ccttcagctt gggtgattct
    11221 tgctaaacca aacttaggca tatcatcacc agatatattt aagttgatta atttagataa
    11281 gcgttacgac gtacatacga aaatgtgtta tgaggcctta gaaaatcgag attatcaaca
    11341 attatgtcaa agtttgtcta atcgattaga gccaatttct gtttcaaaac acccacaaat
    11401 cgataaatta aaaaataata tgttgaaaag tggtgcagat ggtgcgttaa tgagtggaag
    11461 cggacctact gtgtatgggc tagcacgaaa agaaagccaa gcaaaaaata tttataatgc
    11521 agttaacggt tgttgtaatg aagtgtactt agttagacta ttaggataga agggttgaaa
    11581 agatgagata taaacgaagc gagagaattg tttttatgac gcaatatttg atgaaccatc
    11641 cgaataaatt gattccatta actttttttg tgaaaaaatt taaacaggcg aagtcttcaa
    11701 taagtgaaga tgtccaaatt ataaaaaata cattccaaaa agaaaagtta ggtacagtaa
    11761 ttactactgc tggcgcaagt ggtggtgtta cgtataaacc aatgatgagt aaagaagagg
    11821 cgactgaagt tgttaatgag gtcattactc tattagaaga gaaagaacgt ttgttacctg
    11881 gcggatattt atttttatca gatttggtag gtaatccatc gctactaaac aaagttggta
    11941 agttaattgc cagtatttac atggaagaaa aattagatgc tgttgttacc attgcgacaa
    12001 aaggtatttc attggcaaat gcggttgcta atattttaaa tttaccagta gtagtgatta
    12061 gaaaagacaa caaggtgact gaaggttcta cagtttcaat taattacgtt tcaggatctt
    12121 caagaaaaat agaaacaatg gtactttcga agagaacttt agcagaaaat tcaaatgttt
    12181 tagttgtcga tgattttatg agggctggtg gctctattaa tggtgttatg aatttaatga
    12241 atgagtttaa agcccatgta aaaggggtat cagtacttgt agaatcaaaa gaagttaaac
    12301 aaagattgat tgaagattat acttccttag tgaaattatc tgatgtagat gaatataatc
    12361 aagagtttaa cgtagaacct ggcaacagtt tatctaagtt ttcataaaag gagttttagt
    12421 attatgaaaa tcattaacac aacaagatta ccggaagcac ttggaccata ttcgcatgca
    12481 acagttgtga atggtatggt ttatacttct ggtcagattc cattgaatat tgatggacat
    12541 atcgtaagcg ctgatgttca agcacagaca aaacaagttt tagaaaattt aaaggttgtt
    12601 ttggaagaag caggatctga tttgaattct gttgcgaaag cgaccatttt cattaaagat
    12661 atgaatgatt tccaaaaaat aaatgaagtg tatggtcaat attttaatga acacaagcca
    12721 gcgcgtagtt gtgtagaggt tgcgcgtttg ccaaaagatg tgaaagtaga aattgaatta
    12781 gtaagtaaaa ttaaggaatt ataattttcg attaatatgt ttaatcaagc ttctaaataa
    12841 aacagagaga tatatactat aggggggctc actacatgaa agtgacagat gtaagactta
    12901 gaaaaataca aacagatgga cgaatgaaag cactcgtttc cattacatta gatgaagctt
    12961 tcgtaattca tgatttacgt gtaattgaag gaaactctgg cttgttcgtt gcaatgccaa
    13021 gtaaacgtac accagatggt gaattccgcg acatcgcgca tcctattaat tcagatatga
    13081 gacaagaaat tcaagatgca gtgatgaaag tatatgatga aacagatgaa gtagtaccag
    13141 ataaaaacgc tacatcagaa gattcagaag aagcttaatc aattttatat ttagcgatgt
    13201 aatacatttg caataagttg atttgatact gtcgataaag cataaagctt tgtcggcagt
    13261 ttttttagtt tgtattaatg tttttttatt tttaatgaaa ggctaataaa tatatacgtt
    13321 aacagattat gatgatatga aaattattga ttcattgctg ataaaaattg cgtttgaatg
    13381 atgctcgtat ttttgaagta agaaaaaagt tgtttttaaa attacaacga attaaaaaca
    13441 atgcctttta tatgttgaaa gagtattgca gattaaatta taataatgac gaagtgtaaa
    13501 atttaatggg ggttaatgtt catgcgaaga cacgcgataa ttttggcagc aggtaaaggc
    13561 acaagaatga aatctaaaaa gtataaagtg ctacacgagg ttgctgggaa acctatggtc
    13621 gaacatgtat tggaaagtgt gaaaggctct ggtgtcgatc aagttgtaac catcgtagga
    13681 catggtgctg aaagtgtaaa aggacattta ggcgagcgtt ctttatacag ttttcaagag
    13741 gaacaactcg gtactgcgca tgcagtgcaa atggcgaaat cacacttaga agacaaggaa
    13801 ggtacgacaa tcgttgtatg tggtgacaca ccgctcatca caaaggaaac attagaaaca
    13861 ttgattgcgc atcacgagga tgctaatgct caagcaactg tattatctgc atcgattcaa
    13921 caaccatatg gatacggaag aatcgttcga aatgcgtcag gtcgtttaga acgcatagtt
    13981 gaagagaaag atgcaacgca agctgaaaag gatattaatg aaattagttc aggtattttt
    14041 gcgtttaata ataaaacgtt gtttgaaaaa ttaacacaag tgaaaaatga taatgcgcaa
    14101 ggtgaatatt acctccctga tgtattgtcg ttaattttaa atgatggcgg catcgtagaa
    14161 gtctatcgta ccaatgatgt tgaagaaatc atgggtgtaa atgatcgtgt aatgcttagt
    14221 caggctgaga aggcgatgca acgtcgtacg aatcattatc acatgctaaa tggtgtgaca
    14281 atcatcgatc ctgacagcac ttatattggt ccagacgtta caattggtag tgatacagtc
    14341 attgaaccag gcgtacgaat taatggtcgt acagaaattg gcgaagatgt tgttattggt
    14401 cagtactctg aaattaacaa tagtacgatt gaaaatggtg catgtattca acagtctgtt
    14461 gttaatgatg ctagcgtagg agcgaatact aaggtcggac cgtttgcgca attgagacca
    14521 ggcgcgcaat taggtgcaga tgttaaggtt ggaaattttg tagaaattaa aaaagcagat
    14581 cttaaagatg gtgccaaggt ttcacattta agttatattg gcgatgctgt aattggcgaa
    14641 cgtactaata ttggttgcgg aacgattaca gttaactatg atggtgaaaa taaatttaaa
    14701 actatcgtcg gcaaagattc atttgtaggt tgcaatgtta atttagtagc acctgtaaca
    14761 attggtgatg atgtattggt ggcagctggt tccacaatca cagatgacgt accaaatgac
    14821 agtttagctg tggcaagagc aagacaaaca acaaaagaag gatataggaa ataatcattt
    14881 acgtatttaa aatggctagg ataaaaggat aatcctatgt aatattaatg taatctttat
    14941 gatttaatga ttcgcatagt aatggagtta cattttatat ataatagtaa ttgcgtaagt
    15001 aaataattgg aggactataa atgttaaata atgaatataa gaattcgtca ttaaagattt
    15061 tttcattgaa aggaaacgaa gcattagcgc aagaagttgc tgaccaagta ggaattgaac
    15121 taggtaaatg ttcagttaaa cgttttagtg atggagaaat tcaaattaat atcgaagaga
    15181 gtattcgtgg ttgtgacgta tttattattc aaccaacatc atatcctgtg aatctacatt
    15241 taatggaatt attaattatg attgatgctt gtaaacgtgc ttctgcagca acaatcaata
    15301 ttgtagtgcc atattatgga tatgcaagac aagatagaaa agcccgtagc cgtgagccaa
    15361 tcactgctaa attagttgca aacttaatcg aaacagctgg cgcaactcgt atgattgcgt
    15421 tagacttaca tgcaccacaa attcaaggat tctttgatat tccaattgac cacttaatgg
    15481 gtgtgccaat tcttgctaaa catttcaaag atgatccgaa tattaaccca gaagaatgtg
    15541 tcgttgtttc accagaccat ggcggcgtta cacgtgcacg taaattagct gacattttaa
    15601 aaactccaat tgcaattata gataaacgtc gtcctagacc aaatgttgct gaagtgatga
    15661 acattgttgg tgagattgaa ggacgtacgg caattattat tgacgatatt attgatacag
    15721 caggtacaat cactttagct gcacaagcat taaaagataa aggtgctaaa gaagtatatg
    15781 cttgttgtac acaccctgtt ttatcaggac cggctaaaga acgtatcgaa aattctgcta
    15841 taaaagaatt aatcgtaaca aactcaattc atttagatga agatcgcaaa ccatctaaca
    15901 ctaaagaatt atctgttgct ggtttaatcg cacaagctat cattcgtgta tacgaaagag
    15961 aatcagttag cgtattattt gactaatatt taaaaggcgt ttgacgaaca tattccaaac
    16021 gtgtataata gtttcgttcg tgattatacg aataaataaa cacttgcaag caacgatgat
    16081 gttgatgggt aagtgaggtg ctcgttttga gcaaaaatga aaggtggaaa tgagaatggc
    16141 ttcattaaag tcaatcatcc gtcaaggtaa acaaacacgt tcagatctta aacaattaag
    16201 aaaatctggt aaagtaccag cagtagtata cggttacggt actaaaaacg tgtcagttaa
    16261 agttgatgaa gtagaattca tcaaagttat ccgtgaagta ggtcgtaacg gtgttatcga
    16321 attaggcgtt ggttctaaaa ctatcaaagt tatggttgca gactaccaat tcgatccact
    16381 taaaaaccaa attactcaca ttgacttctt agcaatcaat atgagtgaag aacgtactgt
    16441 tgaagtacca gttcaattag ttggtgaagc agtaggcgct aaagaaggcg gcgtagttga
    16501 acaaccatta ttcaacttag aagtaactgc tactccagac aatattccag aagcaatcga
    16561 agtagacatt actgaattaa acattaacga cagcttaact gttgctgatg ttaaagtaac
    16621 tggcgacttc aaaatcgaaa acgattcagc tgaatcagta gtaacagtag ttgctccaac
    16681 tgaagaacca actgaagaag aaatcgaagc tatggaaggc gaacaacaaa ctgaagaacc
    16741 agaagttgtt ggcgaaagca aagaagacga agaaaaaact gaagagtaat tttaatctgt
    16801 tacattaaag tttttatact ttgtttaaca agcactgtgc ttattttaat ataagcatgg
    16861 tgctttttgt gttattataa agcttaatta aactttatta ctttgtacta aagtttaatt
    16921 aattttagtg agtaaaagac attaaactca acaatgatac atcataaaaa ttttaatgta
    16981 ctcgatttta aaatacatac ttactaagct aaagaataat gataattgat ggcaatggcg
    17041 gaaaatggat gttgtcatta taataataaa tgaaacaatt atgttggagg taaacacgca
    17101 tgaaatgtat tgtaggtcta ggtaatatag gtaaacgttt tgaacttaca agacataata
    17161 tcggctttga agtcgttgat tatattttag agaaaaataa tttttcatta gataaacaaa
    17221 agtttaaagg tgcatataca attgaacgaa tgaacggcga taaagtgtta tttatcgaac
    17281 caatgacaat gatgaatttg tcaggtgaag cagttgcacc gattatggat tattacaatg
    17341 ttaatccaga agatttaatt gtcttatatg atgatttaga tttagaacaa ggacaagttc
    17401 gcttaagaca aaaaggaagt gcgggcggtc acaatggtat gaaatcaatt attaaaatgc
    17461 ttggtacaga ccaatttaaa cgtattcgta ttggtgtggg aagaccaacg aatggtatga
    17521 cggtacctga ttatgtttta caacgctttt caaatgatga aatggtaacg atggaaaaag
    17581 ttatcgaaca cgcagcacgc gcaattgaaa agtttgttga aacatcacga tttgaccatg
    17641 ttatgaatga atttaatggt gaagtgaaat aatgacaata ttgacaacgc ttataaaaga
    17701 agataatcat tttcaagacc ttaatcaggt atttggacaa gcaaacacac tagtaactgg
    17761 tctttccccg tcagctaaag tgacgatgat tgctgaaaaa tatgcacaaa gtaatcaaca
    17821 gttattatta attaccaata atttatacca agcagataaa ttagaaacag atttacttca
    17881 atttatagat gctgaagaat tgtataagta tcctgtgcaa gatattatga ccgaagagtt
    17941 ttcaacacaa agccctcaac tgatgagtga acgtattaga actttaactg cgttagctca
    18001 aggtaagaaa gggttattta tcgttccttt aaatggtttg aaaaagtggt taactcctgt
    18061 tgaaatgtgg caaaatcacc aaatgacatt gcgtgttggt gaggatatcg atgtggacca
    18121 atttcttaac aaattagtta atatggggta caaacgggaa tccgtggtat cgcatattgg
    18181 tgaattctca ttgcgaggag gtattatcga tatctttccg ctaattgggg aaccaatcag
    18241 aattgagcta tttgataccg aaattgattc tattcgggat tttgatgttg aaacgcagcg
    18301 ttccaaagat aatgttgaag aagtcgatat cacaactgca agtgattata tcattactga
    18361 agaagtgatc agccatctta aagaagagtt aaaaactgca tatgaaaata caagacccaa
    18421 aatagataaa tcagtgcgca atgatttgaa agaaacgtat gaaagcttta aattattcga
    18481 aagtacatac tttgatcatc aaatactacg tcgcttagta gcgtttatgt atgaaacacc
    18541 ttcgacaatt attgagtatt tccaaaaaga tgcaatcatt gcagttgatg aatttaatcg
    18601 tattaaagaa actgaagaaa gtttaacagt agagtctgat tcgtttatta gcaatattat
    18661 tgaaagtggt aatggattta taggacaaag ttttataaaa tatgatgatt ttgaaacatt
    18721 gattgaaggc tatcctgtca cttatttttc attattcgct acaacaatgc cgataaaact
    18781 aaatcatatt attaaatttt catgtaaacc tgtccaacaa ttttatgggc aatatgacat
    18841 tatgcgttct gaatttcaac gatatgttaa tcaaaactat catatcgtgg ttttggtcga
    18901 aaccgaaact aaagttgaac gtatgcaagc gatgttaagt gaaatgcata ttccatcaat
    18961 aacaaaattg catcgctcaa tgtcatcggg gcaagcagtg attattgaag gcagtttatc
    19021 tgaaggattt gaactacctg atatgggatt agttgtcatt actgagcgtg agctttttaa
    19081 atcaaaacag aaaaagcaac gaaaacgtac gaaagctatc tcaaatgctg aaaaaattaa
    19141 gtcttaccaa gatttaaatg tgggagatta tattgttcat gtgcatcatg gtgttggtag
    19201 atatttaggt gttgagacgc tcgaagtggg gcaaacgcat cgtgattata ttaaattgca
    19261 atataaaggt acggatcaac tatttgttcc agtagatcaa atggatcaag ttcaaaaata
    19321 tgtagcttcg gaagataaga cgccaaaatt aaataaactc ggtggcagtg aatggaaaaa
    19381 aacaaaagct aaagttcaac aaagtgttga agatattgct gaagagttga ttgatttata
    19441 taaagaaaga gaaatggcag aaggttatca atatggggaa gacacagctg agcaaacaac
    19501 atttgaatta gattttccat atgaacttac gcctgaccaa gctaaatcta tcgatgaaat
    19561 taaagatgac atgcaaaaat cgcgtccaat ggatcgcttg ctatgtggtg atgttggtta
    19621 tggtaaaact gaagttgcag tgagagcagc attcaaagct gtaatggaag gaaagcaggt
    19681 tgcattttta gttcctacaa ctattttagc tcagcaacat tatgagacgt taattgagcg
    19741 tatgcaagat tttcctgttg aaattcaatt aatgagtcgt tttagaacgc ctaaagagat
    19801 aaaacaaact aaggaaggac ttaaaactgg atttgttgac atagttgttg gtacacacaa
    19861 attacttagt aaagatatac agtataaaga tttagggctg ttgattgtag atgaagaaca
    19921 acgatttggt gtacgccata aagagcgtat taaaacatta aaacataatg tagatgtact
    19981 aacattgact gcaaccccaa tacctagaac attgcatatg agtatgctag gtgtgcggga
    20041 tttgtcagtg attgaaacgc cgccagaaaa tcgtttccca gttcaaacat atgtattaga
    20101 acagaacatg agttttatca aagaagcttt agaaagagaa ctatcccgtg atggccaagt
    20161 gttttatctt tataataaag tgcaatccat ttatgaaaaa cgagaacaac tccagatgtt
    20221 aatgccagat gctaacattg cagttgctca tggacaaatg acagagcgcg atttagaaga
    20281 aacgatgtta agttttatca ataatgaata tgatatttta gtaacgacga cgattattga
    20341 aacaggtgtc gatgtcccaa atgcaaatac tttgatcatt gaagatgcag atcgctttgg
    20401 attgagtcag ttgtatcaat taagaggtcg tgttggtcgt tcaagtcgta ttggttatgc
    20461 atacttctta catccagcaa ataaggtact aactgagact gcagaagatc gattacaagc
    20521 gattaaagaa tttacggagt taggctcagg atttaagatt gcgatgcgtg atttgaacat
    20581 tcgtggtgct ggtaatttgt taggtaaaca acagcacggc tttattgata cagttggatt
    20641 tgatttgtac agtcaaatgt tagaagaagc tgtaaatgaa aaacgtggta ttaaggaacc
    20701 agaatctgag gtgccagaag tcgaagttga tttaaacttg gatgcatatt tgccaacaga
    20761 atatattgca aatgaacaag ctaaaattga aatttataaa aagctacgaa aaactgaaac
    20821 atttgatcaa attatcgaca ttaaagatga attaattgat cgtttcaatg attatcctgt
    20881 tgaagtagca cgtttgcttg atatagtgga aataaaagta cacgcattac attcaggtat
    20941 cacgttgatt aaagataaag ggaaaataat tgatattcat ttatctgtaa aagccactga
    21001 aaatattgat ggcgaagtgc tgttcaaagc aacacaacct ttaggtagaa caatgaaggt
    21061 tggtgttcaa aataatgcaa tgacaattac tttaacgaaa caaaatcaat ggcttgatag
    21121 tttgaagttt ttagttaagt gcattgaaga aagtatgaga atcagtgatg aagcataaag
    21181 aagcatttaa tggcgttgtc gtgttaactg ctgcattaat tgtcattaaa attctgagtg
    21241 ctgtatatcg aattccatat caaaatatat taggcgatac aggtttgtat gcatatcaac
    21301 aagtgtatcc aattgtagca ttaggaatga tattatcgat gaatgccatt cctagtgcaa
    21361 ttacacaaaa tatagggaag tatcatagtg acgaagcata tgcaaaagca gtcgcttata
    21421 tacaattagt tggtatatta ttatttattg ctatttttgt gtttgcgaac aatattgcac
    21481 atatgatggg tgatggccat ttaacaccaa tgattcaagc tgcaagttta agctttatat
    21541 ttataggtat gcttggcgtg ttaagaggtt attatcaatc tgcaaataat atgacagttc
    21601 cggctatttc ccaggttata gaacaagtta tacgagtagg tattatcatt gttactattg
    21661 ttatttttgt agacagaggt tggacgatat atgaagcggg aacaattgct attttagcat
    21721 caacgatagg ttttttaggt tcttcaattt atttagtagc gcaccgacct tttaagttta
    21781 aaatggtaaa taacactgca aagatcgttt ggaaacagtt cgcactttcg gttttgattt
    21841 tcgctatcag tcaattaatc gtaattttat ggcaagtgat tgatagtgtt actattatta
    21901 agtcacttca agcgatacgc gtgccattcg atgttgccat aactgaaaaa ggagtctatg
    21961 accgtggtgc atcatttatt cagatgggat tgattgtaac tacaacattt agttttgcgc
    22021 tcattcctct gttaagtgac gcaattaaaa tgaataatca ggtacttatg aatcgttatg
    22081 caaatgcgtc attaaagatt acgattttaa taagtacagc agcaggaata ggattaatta
    22141 atttattgcc tttaatgaac ggtgtgtttt ttaagacgaa tgatttaacc ttaacgttaa
    22201 gtgtttatat gattacggtc atttgtgtat cgttaattat gatggatatg gcattattac
    22261 aagcgcaaca tgctgtgaga cctatttttg ttggtatgac ggcaggattg gttattaaat
    22321 ttatacttaa tatcattttg attcgtttaa gtggcattat tggtgcgagc attagtactg
    22381 ttgtatcatt aattatattc ggtacgatta tccatattgc tgtcacgaga aaataccact
    22441 tatatgcgat gagacgattt tttatcaatg ttgttttagg tatggtattt atgtcgattg
    22501 ttgttcaatg cgtgttaaac atagtgacaa cacacggtag aatcactgga ctcattgaat
    22561 tattatgtgc agcagtatta ggtatcattg cattgttttt ctatattttt agatttaatg
    22621 ttttgacata taaagagtta acttatttac catttggttc aaagttgtat caaattaaga
    22681 aaggaagacg ttgatggcac ataccattac gattgttggc ttaggaaact atggcattga
    22741 tgatttgccg ctagggatat ataaattttt aaagacacaa gataaagttt atgcaagaac
    22801 gttagatcat ccagttatag aatcattgca agatgaatta acatttcaga gttttgacca
    22861 tgtttatgaa gcacataacc aatttgaaga tgtctatatt gatattgtgg cgcaattggt
    22921 tgaagctgct aatgaaaaag atattgtcta tgcggttccg ggtcatccta gagttgctga
    22981 gacaactaca gtgaaattac tggctttagc aaaggacaat actgatatag atgtgaaagt
    23041 tttaggtggg aaaagcttta ttgatgatgt gtttgaagca gttaatgtag atccaaatga
    23101 tggcttcaca ctgttagatg cgacatcatt acaagaagta acacttaatg ttagaacgca
    23161 tacattgatt acgcaagttt atagtgcaat ggttgctgct aatttgaaaa tcactttaat
    23221 ggaacgatat cctgatgatt accctgttca aattgtcact ggtgcacgaa gcgatggtgc
    23281 ggataacgtt gtgacatgcc cattatatga attggatcat gatgaaaatg cattcaataa
    23341 tttgacgagt gtattcgtac caaaaatcat aacatcgaca tatttgtatc atgactttga
    23401 ttttgcaacg gaagtgattg atactttagt tgatgaagat aaaggttgtc catgggataa
    23461 agtgcaaacg catgaaacgc taaagcgtta tttacttgaa gaaacatttg aattgttcga
    23521 agctattgac aatgaagatg attggcatat gattgaagaa ctaggagata ttttattaca
    23581 agtgttattg catactagta ttggtaaaaa agaagggtat atcgacatta aagaagtgat
    23641 tacaagtctt aatgctaaaa tgattcgtag acacccacac atatttggtg atgccaatgc
    23701 tgaaactatc gatgacttaa aagaaatttg gtctaaggcg aaagatgctg aaggtaaaca
    23761 gccaagagtt atatttgaaa aagtatttgc agagcatttt ttaaatttat atgagaagac
    23821 gaaggataag tcatttgatg aggccgcgtt aaagcagtgg ctagaaaaag gggagagtaa
    23881 tacatgagat tagataaata tttaaaagta tcacggttaa taaagcgacg tacgctagca
    23941 aaagaagtaa gtgatcaagg tagaattaca ataaatggta atgttgctaa agctggatcg
    24001 gatgttaaag ttgaagatgt gctgacgatt cgctttggtc aaaaattagt aacagttaaa
    24061 gtaactgcat taaatgaaca tgcatctaaa gataacgcga agggtatgta tgaaatcatt
    24121 gaagagcgtc gacttgaaga agcgtaaatt ggaggtgaca agcaatgaaa aataaagtag
    24181 aacatataga aaatcagtac acgtcgcaag agaacaagaa aaaacaacgt caaaaaatga
    24241 aaatgcgtgt tgttcgtagg cgtattacag tatttgcggg cgtattactt gcgataattg
    24301 ttgttttatc aatcttgctt gttgtccaaa aacatcgcaa tgatattgat gcacaggagc
    24361 gaaaagcgaa agaagcacag tttcaaaagc aacaaaatga agaaattgcg ttaaaagaaa
    24421 agttgaataa tctgaatgac aaagattaca ttgaaaaaat tgcgcgtgat gattattact
    24481 taagcaacaa aggtgaagtg atttttaggt tgccagaaga caaagattcg tctagctcaa
    24541 aatcttcgaa aaaataaatc caaattgatt caaaattatc cgagtataga cattgtgaaa
    24601 aaatccaaac aaggatataa taagggaaaa tcgaatcaaa tcgggaggat ttatttaaca
    24661 tatgtcaatc gaagttggaa ataagcttaa aggtaaagtc actggtatta aaaagtttgg
    24721 tgcattcgta gaattacctg aaggaaaaag tggtttagtt cacattagtg aagtcgcaga
    24781 taattatgtt gaaaacgtag aagagcacct ttctgttggt gatgaagtag acgtaaaagt
    24841 attatctatt gctgatgatg gaaaaattag tctttcaatt aagaaagcta aagaccgtcc
    24901 acgtagacaa catacgagta aaccaagtca tcaaaaacca gtgcaaaaag ccgaagattt
    24961 tgaaaagaaa ttaagcaatt tcttaaaaga tagtgaagat aaattaactt caatcaaacg
    25021 tcaaacagaa tctagacgcg gtggcaaagg ttcaagacgt taattaaaat aaataaagac
    25081 tgcttcgata aggaatatat ttagaatgat gcgtatcgaa taatcgattg cagcgttaga
    25141 caatctaaga ctgtttctta aataaggagc agtctctttt atttgtaatg atataactaa
    25201 gacttatacc atttttgaaa attgtaaaag tgaggtgatg ttatgcagtt aaatagtaat
    25261 ggttggcatg ttgatgacca tattgttgtc gctgtttcta caggtattga tagtatgtgt
    25321 ttattgtatc aactactaaa tgattataaa gatagttata gaaaactaac atgcttacat
    25381 gtcaatcatg gcgttaggtc agcttcaatt gaggaagcca gatttttaga agcatactgc
    25441 gaacgtcatc acatcgattt acatatcaaa aagttagatt tgtcgcatag tctcgaccga
    25501 aataacagca ttcagaatga agctcgaatt aaacgttacg aatggtttga tgaaatgatg
    25561 aatgtattag aagcggatgt attgctaacg gcgcatcatt tggacgatca attagaaact
    25621 attatgtatc gtatttttaa tgggaaatca acacgtaata aactaggatt tgatgagtta
    25681 tcgaagcgaa aaggttatca gatttatcga ccacttttag ctgtctctaa aaaagaaata
    25741 aaacaattcc aagagagata tcatattcca tattttgaag atgaatctaa taaagataac
    25801 aaatatgtta gaaatgatat tcgtaataga attattccag ctattgatga aaataatcaa
    25861 cttaaagtat cgcatttatt aaaattaaaa caatggcatg atgaacaata tgatattttg
    25921 caatattcag ctaaacaatt tattcaagaa tttgtgaagt ttgatgaaca gtcaaaatat
    25981 ttagaggttt ctagacaagc ttttaataac ttaccaaact cattaaagat ggttgtgttg
    26041 gactgcctat tatcaaagta ttatgagttg tttaatatta gtgctaaaac atacgaagag
    26101 tggtttaaac aatttagtag taagaaagca caattcagta ttaatctcac ggataaatgg
    26161 ataattcaaa tcgcatatgg taaattaata ataatggcta aaaataatgg cgatacatat
    26221 tttagagttc aaacaattaa aaagccaggt aattatattt ttaacaaata tcgattagag
    26281 atacattcta atttaccaaa atgtttattt ccgcttacag tgagaacacg acaaagtggc
    26341 gatacattta aactgaatgg gcgcgatggt tataagaaag tgaatcgcct gtttatagat
    26401 tgtaaagtgc cacagtgggt tcgggatcaa atgccaatcg tattggataa acaacagcgc
    26461 attattgcgg taggagattt atatcaacaa caaacaataa aaaaatggat tataattagt
    26521 aaaaatggag atgaatagcg ttatgcataa tgatttgaaa gaagtattgt taactgaaga
    26581 agatattcaa aatatctgta aggaattggg agcacaatta acaaaggatt atcaaggtaa
    26641 accattagta tgcgtgggta tcttaaaagg ctcagcaatg tttatgtcag atttaattaa
    26701 acgaattgat acccatttat caattgattt catggatgtt tctagttatc acggaggcac
    26761 tgagtcaact ggtgaagttc aaatcattaa agatttaggt tcttctattg aaaataaaga
    26821 cgtattaatt attgaagata tcttagagac tggtactaca cttaagtcaa ttactgaatt
    26881 attacaatct agaaaagtta attcattaga aatagttact ttattagata aaccaaaccg
    26941 tcgtaaagcg gacattgaag ctaagtatgt aggtaaaaaa ataccagatg aatttgttgt
    27001 tggttacggt ttagattatc gtgaattata ccgaaactta ccatatatcg gtacgttaaa
    27061 acctgaagtg tattcaaatt aattttttaa tcaatttcag ttattattac tatgcgtttg
    27121 agaaataata gtgtagactc aaaaatatga aaaatgtatt tcatatatat ttaattttag
    27181 acaagacata tgtcttgaaa agttgaaaaa tatagagatt gataaaacta atacgggtgt
    27241 gaatgacatt gatgttaagc tcaattacta gcttataaaa catgtcatat gttacaattt
    27301 ttgttagttt tattatggga agtaggagga aatgacgcat gcagaaagct tttcgcaatg
    27361 tgctagttat cgtaataata ggcgttatta tttttggtct attttcatat ttaaacggta
    27421 atggaaatat gccgaaacag cttacatata atcaatttac tgagaagttg gaaaaaggtg
    27481 accttaaaac tttagaaatc caaccacaac aaaatgtcta tatggtaagt ggtaaaacga
    27541 aaaatgatga agactattca tcaactattt tatataacaa cgaaaaagaa ttacaaaaaa
    27601 ttactgatgc tgctaaaaag caaaacggtg taaaattaac gattaaagaa gaagaaaaac
    27661 aaagtgtctt tgtgagtata ctttcaacat taattccagt tgtagtcata gcgttattat
    27721 ttattttctt cctaagccaa gcacaaggtg gcggtagtgg cggtcgtatg atgaactttg
    27781 gtaaatctaa agcaaaaatg tacgataata ataaacgtcg tgttcgtttc tctgatgtag
    27841 caggggcaga tgaagaaaaa caagaattaa ttgaaattgt tgatttcttg aaagataata
    27901 aaaaattcaa agaaatggga tctaggattc ctaaaggtgt cttacttgtt ggacctccag
    27961 gtactggtaa aacattactt gctagagcgg ttgcaggtga agctggcgca ccattcttct
    28021 ctattagtgg ttcagacttt gtagagatgt ttgttggtgt tggtgcgagc cgtgttcgtg
    28081 acttattcga taatgctaag aaaaacgcgc cttgtatcat ctttatcgat gagattgatg
    28141 ctgttggtcg tcaacgtggt gcaggtgttg gtggcggtca tgatgaacgt gaacaaaccc
    28201 taaaccaatt attagttgaa atggatggtt tcggtgaaaa tgaaggtatc attatgatag
    28261 ctgctacaaa ccgtcctgat atccttgacc cagccttatt acgtccaggt cgttttgata
    28321 gacaaattca agttggtcgt ccagatgtga aaggccgtga agcaattctt catgttcatg
    28381 ctaaaaacaa accacttgat gaaacggttg atttaaaagc aatttcacaa cgtacacctg
    28441 gtttctcagg tgctgattta gagaacttat taaatgaagc atctttaatt gctgtacgtg
    28501 aaggtaaaaa gaaaattgac atgagagata tcgaagaggc aacggataga gttatagccg
    28561 gacctgctaa gaaatctcga gttatttcta agaaagaacg taatattgtt gctcatcacg
    28621 aagctggtca tacaattatc ggtatggtac ttgatgaggc agaagtagtg cataaagtta
    28681 ctattgttcc acgtggacaa gcaggtggtt atgcaatgat gctacctaaa caagatcgtt
    28741 tcttaatgac tgaacaagag ttattagata aaatctgtgg tttacttggt ggacgtgtat
    28801 cagaagatat taactttaac gaagtatcaa caggtgcttc aaatgacttc gaacgtgcaa
    28861 cacaaatcgc acgctcaatg gttacgcaat atggtatgag taaaaaatta ggaccattac
    28921 agttcggtca tagcaatggt caagtattct taggtaaaga tatgcaaggt gagcctaatt
    28981 attcaagcca aatcgcatat gaaattgata aagaagttca acgaatcgtt aaagaacaat
    29041 acgaacgttg taaacaaatt ttattagagc acaaagaaca attaatttta attgctgaaa
    29101 cattattaac agaagaaaca ttagttgctg aacaaattca atcattattc tacgaaggta
    29161 aattacctga aattgattat gatgcagcta aagttgttaa agatgaagat tctgaattta
    29221 atgatggtaa attcggtaaa tcttatgaag agattcgtaa agagcaatta gaagatggac
    29281 aacgtgacga aagtgaagat cgtaaagaag aaaaagatat tgctgaggat aaaaaagaag
    29341 ctgataaatc tgatgaaaaa gatgaaccag cacatcaaca agccccaaat atcgaaaaac
    29401 cttacgatcc aaatcaccca gacaataaat aatcgattat attcagtacc tctttctatg
    29461 ataaagttat agaaagaggt acttttatcg tttttgaaaa tacgtattag attttaagtc
    29521 gttgaattgt tatagcagaa aataattgta aaacaagtta cttcattatt tagaatgatg
    29581 ggtgtagaat aagtacaatt gttgcatttt atgaagtaaa gtaatttttt aaatatagag
    29641 taatagagga gattgaaata atgacacacg attatattgt taaagcatta gcatttgatg
    29701 gagagattag ggcttatgct gctttgacaa ctgaaactgt tcaagaagca caaacgagac
    29761 attatacatg gccgacagca tctgctgcaa tgggaagaac aatgacagca acagctatga
    29821 tgggcgcaat gttgaaaggt gatcaaaaat taactgtcac tgtagatggc caaggaccta
    29881 ttggacgaat tattgccgat gcaaatgcta aaggcgaggt gcgtgcttat gtagaccatc
    29941 cacaaactca ttttccatta aatgagcaag gtaaacttga tgtaagacga gcggtaggga
    30001 caaatggatc tattatggtt gttaaagacg ttggaatgaa agactatttc tctggagcaa
    30061 gtccaattgt ttcaggagaa cttggtgaag attttactta ttattatgct acaagtgaac
    30121 aaacaccttc atcggtaggt cttggtgtat tggtaaatcc tgataatacg attaaagcag
    30181 caggaggatt tatcattcaa gttatgccag gtgccaaaga tgaaacaatt tcaaaattag
    30241 aaaaagcaat tagtgaaatg acaccagttt ctaaattaat tgaacaagga ttaacgccag
    30301 aaggattact aaacgaaatc ttaggtgaag accatgtgca aattttagag aaaatgcctg
    30361 ttcaatttga atgtaattgt agtcatgaga aatttttaaa tgctattaaa ggattgggcg
    30421 aggctgagat tcaaaatatg attaaagaag atcatggtgc tgaagcagta tgtcatttct
    30481 gtggaaataa atataaatat actgaagaag aattaaacgt gttgctagaa agtttagcgt
    30541 aatttaattt aaatc
//
LOCUS       DAKTIP010000010        29327 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAKTIP010000010 DAKTIP010000000
VERSION     DAKTIP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 29327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.010"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O0305_000343"
     CDS             complement(74..1954)
                     /gene="vraE"
                     /locus_tag="O0305_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCY0273529.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFKSALFLKRRSILSMMKDSIKTDATTAKVTTAEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVNDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNIINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVIEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O0305_000344"
     CDS             complement(1944..2702)
                     /gene="vraD"
                     /locus_tag="O0305_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCY0273530.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            2985..3497
                     /locus_tag="O0305_000345"
     CDS             2985..3497
                     /locus_tag="O0305_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCY0273531.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIVYRRKAKALVGGNQNWILFMGIVDILFGLLVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            complement(3631..4425)
                     /locus_tag="O0305_000346"
     CDS             complement(3631..4425)
                     /locus_tag="O0305_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCY0273532.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(4668..5684)
                     /locus_tag="O0305_000347"
     CDS             complement(4668..5684)
                     /locus_tag="O0305_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCY0273533.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            complement(5849..6424)
                     /locus_tag="O0305_000348"
     CDS             complement(5849..6424)
                     /locus_tag="O0305_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0273534.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVQSSLPFKLTGDVSCNHAPKRQQMIDFIVVTAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVG"
     gene            6649..7557
                     /gene="rarD"
                     /locus_tag="O0305_000349"
     CDS             6649..7557
                     /gene="rarD"
                     /locus_tag="O0305_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCY0273535.1"
                     /translation="MNSEYKKGIFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDMNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            complement(7970..9388)
                     /locus_tag="O0305_000350"
     CDS             complement(7970..9388)
                     /locus_tag="O0305_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCY0273536.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVVALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O0305_000351"
     CDS             complement(9626..10090)
                     /gene="bstA"
                     /locus_tag="O0305_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCY0273537.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPER
                     ARNLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            10257..10595
                     /locus_tag="O0305_000352"
     CDS             10257..10595
                     /locus_tag="O0305_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCY0273538.1"
                     /translation="MSVSDQIMKGLLDGAILGLIAQGETYGYEIMTKLKNQEFPEISE
                     GSIYPVLMRLNKKGYVDTTIKKSSNGGPRRKYYTITDSGLKELKVFKSKWQVLNKGMI
                     NLFGGENDVK"
     gene            10585..11352
                     /locus_tag="O0305_000353"
     CDS             10585..11352
                     /locus_tag="O0305_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273539.1"
                     /translation="MLSKQSQDFLEKLRVELLFRGKTEAEVEELLEELEDHLTMAEKN
                     NEDTSSIINTPIKSMADQLSPEISLTTGLYKYITLYFAFILAVIIIPRFLDLGTFDVT
                     IAFLLYIASIVILGVVIGMLILRNTLVRFGDQKITYVINVTYGIIVFLWMILGGLIIK
                     KYPIYSFFELSDKQSFIVGLVLLIALIGTCILIKQKIYALIVLVISLPSLIARIAAIF
                     SKEPDTFNLVSVSILIILNIAFIIYTFVNFRKLKKED"
     gene            complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O0305_000354"
     CDS             complement(11471..12109)
                     /gene="pcp"
                     /locus_tag="O0305_000354"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCY0273540.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHTLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(12261..13217)
                     /locus_tag="O0305_000355"
     CDS             complement(12261..13217)
                     /locus_tag="O0305_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCY0273541.1"
                     /translation="MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            13461..14432
                     /locus_tag="O0305_000356"
     CDS             13461..14432
                     /locus_tag="O0305_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCY0273542.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKRPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(14581..15096)
                     /locus_tag="O0305_000357"
     CDS             complement(14581..15096)
                     /locus_tag="O0305_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCY0273543.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            15476..16324
                     /locus_tag="O0305_000358"
     CDS             15476..16324
                     /locus_tag="O0305_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCY0273544.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREVTKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            16353..16907
                     /locus_tag="O0305_000359"
     CDS             16353..16907
                     /locus_tag="O0305_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCY0273545.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMVYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            16960..18672
                     /locus_tag="O0305_000360"
     CDS             16960..18672
                     /locus_tag="O0305_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273546.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLKDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIAALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTV
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            18669..19502
                     /locus_tag="O0305_000361"
     CDS             18669..19502
                     /locus_tag="O0305_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0273547.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLVKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            19549..20046
                     /locus_tag="O0305_000362"
     CDS             19549..20046
                     /locus_tag="O0305_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273548.1"
                     /translation="MINTERLNLMIPSSSHLIELYNICSHPQANIYTPKGLHNSKLDT
                     QRWIEKWRNHWQQYQFGYFVLVKKIDCSVIGICGYEYRQLKQETVLNLFYKLHPSFEG
                     QGYACEAITAITNFVNYIDQETVKVIRTNKCNQRSINLAERLKFKRDDTMDDIINQGD
                     IVFYK"
     gene            20164..21288
                     /locus_tag="O0305_000363"
     CDS             20164..21288
                     /locus_tag="O0305_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCY0273549.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRRYFKGNRDYEE"
     gene            21593..22411
                     /locus_tag="O0305_000364"
     CDS             21593..22411
                     /locus_tag="O0305_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCY0273550.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            22429..23043
                     /locus_tag="O0305_000365"
     CDS             22429..23043
                     /locus_tag="O0305_000365"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCY0273551.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            23036..24286
                     /locus_tag="O0305_000366"
     CDS             23036..24286
                     /locus_tag="O0305_000366"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCY0273552.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            24302..25315
                     /locus_tag="O0305_000367"
     CDS             24302..25315
                     /locus_tag="O0305_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCY0273553.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMRTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETRQFLTMQRQLAEQLKQIFDTHVADKMSVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATASQLKQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            25284..25862
                     /gene="hisB"
                     /locus_tag="O0305_000368"
     CDS             25284..25862
                     /gene="hisB"
                     /locus_tag="O0305_000368"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCY0273554.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            25859..26437
                     /gene="hisH"
                     /locus_tag="O0305_000369"
     CDS             25859..26437
                     /gene="hisH"
                     /locus_tag="O0305_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCY0273555.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            26430..27134
                     /locus_tag="O0305_000370"
     CDS             26430..27134
                     /locus_tag="O0305_000370"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCY0273556.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            27131..27889
                     /gene="hisF"
                     /locus_tag="O0305_000371"
     CDS             27131..27889
                     /gene="hisF"
                     /locus_tag="O0305_000371"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCY0273557.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTTGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            27886..28518
                     /locus_tag="O0305_000372"
     CDS             27886..28518
                     /locus_tag="O0305_000372"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCY0273558.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            28711..28890
                     /locus_tag="O0305_000373"
     CDS             28711..28890
                     /locus_tag="O0305_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273559.1"
                     /translation="MKSKLSNSGIDVTVKDVEKYMNRYNEVMKGKNGEKAKELCLSLL
                     PINIIVVFTFFVFIL"
BASE COUNT        10151 a         4443 c         5310 g         9423 t
ORIGIN      
        1 ttaaatcaat taatagttat actgcattgc ttaaaatcaa aatatctaat gaagcatctt
       61 ttaatcgtaa gtgttaaatg gttttcttaa tcaatttgtt tgaatgtaca aaagcaatta
      121 aagcaaaagt aatgtaaatg agggtgtata caacaattac tacaataacc ggcatgaagg
      181 aaatattacc catcaatttc ataaatgcta ttgcggcgaa tactgcatgt aatatagcaa
      241 tcaataatgg taaaccgaag ttgaaagtaa tttttaataa taatccctta agcatatctg
      301 tatgcgtaaa gcctatgcgt tttaatattc tgaagttact tagttcatcc tcagtttcat
      361 ccatttgttt aatataaata atacatccag ctgctactaa aaatgctaat cctaaaaatg
      421 atgtaacaaa tattagaata ccgttagtag catcgacctc ttttttcatt tcatcatacg
      481 tgatgacttt gtctccaaac tgttttgcaa ttgcttgagc tttttccttt tgtgatgttt
      541 gtttaatatc atatccataa aaagtatgaa cgttattttg tgttttcaac tgctgatact
      601 tttcaggact tacttcgatg acaggtgagt tgaagcttag atttaaagga taaaccttac
      661 ctttgtcttc ttgtgtaaca cggaaagttt cattcttagt accttttact actaaatctt
      721 tgtttaaatg gatattaata atgttaggca gcgattttgt atttgtaatg atggcattgt
      781 tgcctgttaa cttgttattt gcacttaaaa tagaattcgt gcgacctgaa tcactaccat
      841 tttccaaagt aataacctga tcattaacat tatctacagt aatagtttcg taagcatttt
      901 tagaaaatgt aatttgttgt tggcttagtt tagtttcaaa ttgtttagca tcttgagtag
      961 cgaccacgtt aaattcattt ggtgccatag atgtaagggt ttgatctgta tttgatttag
     1021 ataatgccgc aaaacacaat acagttactg taactgcaga aataattgca atgatagtta
     1081 aagacatggc atttttcttc attctgtaca taatagacga tgtgaataca acatcggtaa
     1141 tagatacgcg tccatttttt gattttttca atgttttaaa aataagtgac acggaacttc
     1201 tgaagaataa ataggcgcct acaaccgtta aaaataaaat gataaacggt gatgtcatag
     1261 ccatagttag tgctttgaac gtaccaaaca tttctgtcgc catataatag cctagtgcaa
     1321 tcatagcaat acctaatacg cctgaaataa cctctgcagt cgttacttta gcagttgtgg
     1381 catcagtttt aattgaatct ttcatcattg ataagatact acgtcttttt agaaataaag
     1441 cacttttaaa taaaatcagt acatacgcaa taattagcat gaaaatagtt aaaacaaggg
     1501 ccataggttc gaaatgtatc gataagttaa tcgataacga catcaattta gatactatgg
     1561 aaagcaataa ttgtgcacct gcaatgccac ataatacacc gacaacacct gtgattaaaa
     1621 atacgatcat ttgttcaagt gctaacattt tcaaaatgtt ttgtcgtgtt aaaccaatca
     1681 actgaaatag cgcaaattca cgtgtacggc gttttacgaa taaatgattg gcatacatta
     1741 aaaagatgac aataatgata aataaaaata ttgatccgac taaagcacct ttcttaatga
     1801 tggccatcga gtcgtcatta tttacacctt tagtaaactg taaggttgta aaactgaaat
     1861 ataagacgat gctaaaaaat aatgaaaata aatacattgc ataatgtttt aagttttgtc
     1921 gtaagttttt gaaaacgata tggttaaatg tcatttgaga caccacctaa tactgattga
     1981 agatgtacaa tgtcttcata aaaggcctgt ttagaacgtc cttcctgata aagttgtgta
     2041 tgaatttggc catcttttag catgatgaca cgttctgcaa aacttgctgc aaccggatca
     2101 tgtgtaacca tgacaatagt tgtatcaaac gatttattca tttcttccaa acgttgtaat
     2161 aggtcatttg cacttttcga gtcgagtgcg cctgttggct catctgcaaa tatgatttgt
     2221 ggtttgtgaa caaatgctct cgctgctgca gttctttgtt gttgaccacc agataattcg
     2281 ctagggtatt tatttcctag gtcataaata cctaatgctg tcgtgatcgc tttataattt
     2341 tcttccattg ttgccttcga cattttttga acagataaag gtaacataat gttttcttta
     2401 acggttaatg tcggcagaat actgtaatct tggaagatga aacctaatga ttctttgcgg
     2461 aatttggcaa gtgctttttg attaagttta ttaagctctt gtccgttagc aatcacgcta
     2521 ccgctagaaa tttggtcaat tgaacttagt acatttaata aggttgtctt acctgatcca
     2581 gaaggcccca taatcgcaac gaattcgcct ttttgtatgt caaagttaat atctttaagt
     2641 gcttgaaatg tgtgcttttt accgtatgtt tttgaaacat gttgcactga taatatcgtc
     2701 atagtctcac tccttttgta tttaatttca ttttaaataa tgtttggagt agtagccttt
     2761 atctaaactt acaattcaat gaatgaacct tacagagttg aaagctatcg ctacttagta
     2821 gatttttgag tgaggataca gattcatcgt acatattaga caaaagcaat ggtgctttct
     2881 aagtgatgat gtttgtgtaa attgagaaaa gggaatttaa ttattgtata ataaattttt
     2941 tgtaaaaatt aaaagagggt tttatttgaa aggaattgat tgttatggaa aaaggaaatc
     3001 aaggtattaa atggtctagt ttaataatgg gtgtattatt attaatgttg gcagtcgtta
     3061 tttttacatt tccaattgaa aatttttatg ctattacctg gttgattgga ctgtttgtat
     3121 taattaacgg tgtgattcaa atcgtttacc gtagaaaagc aaaagcttta gtaggtggta
     3181 accaaaattg gattctgttt atggggattg tagatattct atttggtcta ttagttattt
     3241 ttaatgttgg cgcaagttca gcattcttta tttatatgtt tgctttttgg tttattttta
     3301 gttctatctc tggattattt acgttttcgg gtagtggtag cttaaaacta atttcagtga
     3361 tttttaattt attaggtatt gttttcggtg tcattttatt atttaatcca ttaatgggta
     3421 tcgtctttat ttcgacgatg attgctattg catttgtatt cgtaggtgtc atttatgttg
     3481 tagatgcact tgcttaagta aaatgaagcg gttcaaaaga agggtgtgac atgaagtttg
     3541 tgtcatatcc tttttgttgt gtttatgaag cataaaaaag gggcgctacc tacaataagt
     3601 aagatacgcc catattttta tattttacta ttattgtttt tcaatacgat taatagttac
     3661 atttagtcca aaatattttt ctaaaaaatg tttatagtta tctttagtga catcaaattt
     3721 ttctgagcta ccattccttg ttaaagttaa atgattttca gacattgtag cacggccaaa
     3781 tgattgtggc attgtaatta ataaatgctg tacaaatatt gaatctggat gcgtttgatt
     3841 atattcgatg ttgctattaa aatcttctat ttgtttaggt ttgaattcag cttcatattt
     3901 tgtatgccaa tgatcatttt cgaatttttg aacatagaaa atatccttgt cttcgttgtt
     3961 aaaaatagcg cggaatgtac cactgatatc agtaattggt tgtgtatgct cagatgaagt
     4021 aataggaatg gcatgtagag gtaagtctcc aaagccaaca tcagttacat agaatacatc
     4081 atttatagaa acaacaagtg aagcatgtga accgttcaga ctacgaccgc caccgggtgt
     4141 gtgaatagta gctgacatta attcaggatt aaatcctttt tgttgtaaat aggctttgaa
     4201 aaatgtattt aattcataac aaaaaccacc acgtttatca tgaacaattt tattaaaaag
     4261 tgcatcgata tttatagata tcggcttact attttgaaca tcaatatttt caaaaggtac
     4321 agttaacata aaacgtgttg cataataatt taatgcttca atactcggtc gattataacg
     4381 agatgaatca atttgtaaat aattctctaa cttcgcaata ttcataagca tagcgcctcc
     4441 tgtattaaag attataatta aattttaaac agaaatactg aaattttaaa ttcgaaagca
     4501 ttgaattttg gataaataca ttttaaatag aaaaatacgc tctcaaaatg aagtcatctc
     4561 taaaagaaac gatttaaaga tgactactga gagcgtagca taatggaaga agtgtgcagg
     4621 gtgtctaaaa atgcaacaat acaaaggtag ttgcaagaca agttgcctta tctagaccat
     4681 ttgtgttcta tgcgaccaaa cttccaaatt aaacttgaaa taagccaagt aattaaaaat
     4741 aatgcaacta aaatatagcc taaataatca aattcgatcg aaccaatgaa tgcccaaaac
     4801 gcaccatgta aatctaactt atcagcaaga atttgtagca attcaatcat cccaatcact
     4861 aatgctgcca tgactgatat cgcagtaatc gttatattgt aatagatttt gcgaatagga
     4921 ttgaagaatg cccaattata ggcatacttc attacaacac catctaatgt atccaataaa
     4981 ctcatacctg atgcgaataa aattggtaaa gataagattc cgataaatga aatggcttgt
     5041 tgtgatgcgc ctgaagaaag agcgagtaac gcaatttcac tagctgtatc aaaaccaagt
     5101 ccaaataaaa agccaagtgg caatacgtgc caactacgcg tgattaattt gaaataaggt
     5161 cctacaaatc gagaaaccaa tcctctagat tcaagtaatg catcgacttc agcttcttca
     5221 atgtgttcac gacgtaattt agcgaacaag ttaattaaag agattaaaat aattagattc
     5281 aacacaccga taagcactaa aaagaaacct gaaactagtg taccaatcgt tccaccaata
     5341 tcttggaaat gcggtaattc atctttagcc cattttacag atacccctaa aaaaacagcc
     5401 attaaaaata cgacagatga atgtccaatt gaaaaataga aacccacacc agatggatct
     5461 ttgcgttgct gtaataattt gcgaaccgta ttatctattg cagcaatgtg atctgcatca
     5521 aatgcatgac gcaaacctaa tgtatatgca agaatcccca taccaaataa gatatgatgg
     5581 tcttttccag caatccataa aaaactaaac ccaataacgt gtaacaaaat gacaatagct
     5641 atgtatggta accagcttaa gcgctcattt ttaaaaacag tcaaaatata tccttctttc
     5701 ataacatgat atttaatcat actgtatgtt caatgggcac tctagtaata agtgttcata
     5761 taacaaaaat gttatgccaa attatttgtt atataaaaat atacatgtaa ccacaaaaga
     5821 ttttttgcga tatatataat ttgataaatt aaccaacaac aatgtaagat gtcactttgc
     5881 ttaacttggc atccttttta tgattttcaa attcaaaaaa atgagcaaaa tgaatctctt
     5941 taccagtttt taatatttca ataccatgca tggaacctaa gcacccatgt gtgatgctgg
     6001 aatggatatt gagactagca acctgattgt aatgattaga tagttcttga attaatattt
     6061 gaggtccgta tatgtcaaag cggccaggga cagaccaaat aaattctgtt gtaaccagtg
     6121 aacgtaataa ttcaatatct aatgctgctg taacaactat aaaatctatc atttgttgac
     6181 gtttaggcgc atgattgcat gacacatctc ctgttaactt aaaaggtaat gatgactgaa
     6241 cttccgtttt aaaatgtagt tggtgctgaa ataaagcttg ttccaatatt gatatagctt
     6301 taggaccaac gccatgcatc tccattagag atgacttcgt atattgtgat actgcttcta
     6361 atgtgtatat accttgtgaa tttagcgcgc gtgttgcagg ctttccaatt ttaggtaaag
     6421 tcatttgtat ccatccttta tgttgtatgg tagtctttct ggcaatatac cctgatttgt
     6481 acagaattat ttataaaatt aatattgttt agtggttggt gaataaatgt gggaaatata
     6541 aacaaaatga tatctttatc gaaatattat tagtaaaatc gttatattag cataaccaag
     6601 tgagcgtatg cagtaaatta aaaattagga aagtaaggag gtttgaaggt gaattcagag
     6661 tataaaaaag gcatattttt agcactcagt gcatacattc tgtggggaat actacctata
     6721 tattggcagt tcgttgatgc aataggcgca tttgaaattt tagcctttcg tattatattt
     6781 tcagcaatat tcatgatttt catactcgcg gttggacaaa aacaacgcaa tgcatttcaa
     6841 cgagatatga atcaattgtt aggcaagccc attcagctat tagcgattgt cgtagcaggc
     6901 tatgtcatta cattaaattg gggtacattt atttgggctg taacgaacgg tcacgtccta
     6961 caaacaagtt taggttatta tataaatcca cttgttagca ttttgctcgc acttatcttt
     7021 ttaaaagaaa gattcaataa atttgaatgg ctagccattt tattcgcatt catcggtgta
     7081 ttatatatga cgctcaagat tggagaattc ccaatcgtct ctattatatt agcgttatcc
     7141 tttggtacat acggattatt gaaaaaagta gtacatattg atgccatcag cagtattacg
     7201 attgaatgta ttgttaccgc acctgctgga ctaatatacg ttatttattt atggcagcaa
     7261 catcagatgt catttggatt gaacatgtca tcattttggt tgttattttc tggtgctatt
     7321 acggcaatac cactaatcct attctcagcc ggggcaaaac gtattccact ttcgctaata
     7381 ggatttattc aatacgttgg accaacaata atgtttgtac tcggcatatt tgttttcaaa
     7441 gagcctttta gtatagatca attaattacg tttatattta tttggacagg tattgtgtta
     7501 tatagtcttt ctcaatacat taaattgaag aaacatccgg tcgcaaaaac cctataaaat
     7561 acgaggaaag ataattatac gtgttttcaa tatcctttgt gtcatgatga ggtgagactg
     7621 aggtatgtgt gcagtaacag tgacagacgt ataaatttga aacattaaat atgaaataag
     7681 gcactttcgt tgaattcgca attaaaatga atttaaacga aggtgttttt tgtatgatga
     7741 tttatgaggg catacgtaat gttaatcaat gaagcggaat agtatagaaa tgtattttat
     7801 gtgatgttac gtaatgactt aaatctttat acaaattttc agtgaagcac gcaatgattt
     7861 aaaggataaa cgtattttaa aaagggagtc ttgatttagc aaaggttgat tttaaataca
     7921 aattaaaaag cgcccaaatc aatgagattc gagcgctaat ttaaatattt taaaatatac
     7981 caattacttt catccaaagt gatcctaaac caatccagat aataaagtag acgaaaccta
     8041 atattaagtt cattgtccac caacgttttt gagtcacgta acctgaagag aatagaatcg
     8101 gcgctggacc actactatag tgtgttgttg aagctaatag gttaccgaag aaacctaaca
     8161 ttaatgcact gaataatggt ggtgcaccgg ctgcgatggc aacgcctagt aatgctgcat
     8221 acatcgcact gatatgtgct gtagaacttg caaataagta atgtgaatag aagtagaaca
     8281 atattaaaat gaccaggact ataggccagc ttaagccacc aagacttgta gcaatggatt
     8341 tacttaacca aggaataaat ccaagcttgt ttaattggtc ggccattaac actaatactg
     8401 agaaccatac taatgtgttc caagcacctg tttcgtttaa aatgtcttgc catgttaaga
     8461 cgcctgtcaa taataacaat gctagcgcaa taaaggccgt taaagttgca tcaatatgaa
     8521 tgaaacttcc gacaatccat agtgttaacg caacgacaaa aataccaatc ataaattttt
     8581 cagctaaagc gattttaccc atagtcgcta attcattttc agcccaactc ttagcatttg
     8641 gtgtttcttt aacagttggt ggataaattt tataaataat aaaaggtaca acaattaagg
     8701 aaacaagtcc aggaactaaa gcagctagaa accaattcat ccatgtaatg tgaacattag
     8761 atgtgctaga tgctaaattt tgtgcaaggg ggttaccggc cattgcagtt aaaaacatag
     8821 ccgcagtaat taaattacct tggaattctg tgaaaacaag aaatgcaccc attttgcgtg
     8881 ctgatccgtc tttcggtttc gaaccaaatg attcagaaag tgatttgata attgggaaca
     8941 tgattccacc agcacgcgcg gtattacttg gtgtagcagg cgctagaatt aaatctacac
     9001 cgacgataga atatgctaat cctaatgttt ttttaccaaa taatttgacg aaatgaagtg
     9061 cgatacgtct accaagacct gttttcacaa atcctctcga aatgaaaaat gccatagcaa
     9121 ttaaccaaat gctattatta ccaaaaccag cgacagccgt tttcatgtca acaatgccaa
     9181 cgagtaccat gattgtaaat ccaattatag agacggcccc aattggcatc ggttgtgtaa
     9241 tacaagcaat gattgtcgcg acgaatattg cgaacatata ccatgctgtt ggatccacag
     9301 catccggttt aaaaggtgta agtgcccaaa taaggagacc tacaacgata gggagtataa
     9361 acttacgata tttaaccgtg ttttccatgt taaaacgtcc ttctttctat gttttataca
     9421 tatttcaatt taagaataaa gctaactaca aaagatgtac agtaataatt aaatataaaa
     9481 ttaaattaac gaaatcatta atataattat ttttcgagaa tcggtgaaga actggtatag
     9541 atggtgttta ttaaattcaa aaaagtgacc ttttaaacaa ataaaaaacc cgttaacgtg
     9601 ttgttaacgg ttaataagca catatttatt ttaaattttt taatatacga gacatctctt
     9661 caattttacc agcatgaagt gggatgtgaa tggcatttaa tactaataac tcttctaagt
     9721 tattacaacc gacaattggc tgtttcaatt caattgctaa atcatcttca gttagatttc
     9781 gtgcacgttc aggtaaagtt tggagacctt ctaaaatcct ttcaatactc ggtacttcat
     9841 ccttccattc atctggagac gaaccatttc cgaataaagg tctatagata tttaaatcaa
     9901 tattctcttt accagcaaca gctaaggccg attcaaaaat tgttaataca tgaccaaatt
     9961 gccaatgaat ggtattagaa aaaggcttat gataatcatc aagtaccttt tcaactttcc
    10021 aagcatcata cacatttact aaatatccta accccatatc aatgacatca aatactgttt
    10081 ttgtagtcat tattaattcc tcccccataa ataaaatata cttccgtaag tattacccta
    10141 ttaacaaagt tgttagtcat ttaaggaatg tgtacaacaa ctaccttaca ataaaagata
    10201 gttgacgaaa agttctcaat gtactatctt attatgtaag atagtaggtg attaaaatgt
    10261 cagtgtcaga tcagattatg aaaggtctct tagatggggc gatattaggt ctcattgccc
    10321 aaggcgaaac gtatggatat gaaattatga cgaaacttaa gaatcaagag tttccagaga
    10381 taagtgaggg tagtatttat ccagttttaa tgcgtttaaa caaaaaagga tacgttgata
    10441 cgacaataaa aaaatctagt aatggcggcc cgaggcggaa atactatacg attaccgatt
    10501 caggtttaaa ggaactaaaa gtatttaaat cgaaatggca agttttgaat aaagggatga
    10561 ttaacttgtt tggaggggaa aacgatgtta agtaaacaat ctcaagattt tttagaaaaa
    10621 ttacgtgttg aattgttatt tagaggtaag actgaagcag aagtagaaga gttattggaa
    10681 gagctcgaag atcatttaac tatggctgaa aaaaataacg aagatacaag ttcaattatt
    10741 aatacaccga ttaagtcaat ggctgatcaa ttatcacctg agataagtct tactaccggt
    10801 ttgtataaat atattacttt gtacttcgct tttattttag cagtcattat aattcctagg
    10861 tttttagatt tgggtacatt tgatgtgaca attgcatttt tactttatat agctagtatc
    10921 gttattttag gtgttgtcat tggtatgctg attctacgaa atacattagt ccgatttggt
    10981 gaccaaaaaa taacgtatgt tataaatgtg acttatggaa ttatcgtatt tttatggatg
    11041 attttaggtg gcttgattat taaaaaatat cctatctata gtttctttga gttaagtgac
    11101 aaacagagtt tcatcgttgg gttagtactt ttaattgcgc tcattggaac ttgtatttta
    11161 attaaacaga aaatttatgc gctcatcgtg ttggtaattt cattgccaag tttgatagca
    11221 aggatagcag ctatctttag taaagaacca gacactttta atttagtttc agtaagtata
    11281 ctcatcattc taaatatagc atttattatt tatacatttg taaattttag gaagttaaaa
    11341 aaagaagact aaacaatgtc gtttagctaa aatacatcat tatagtaaaa ctgtatacca
    11401 atcatccaaa agcaccctaa agttagactt aaactaacaa attagggtgc ttttgtttgt
    11461 gcacttataa ctattccgtt gtgcccaggg ctaggtgtaa atcctcatca ttagaaatag
    11521 cttcaatggc tgcagtcaaa cctgcaacta tcttttctaa tggcatagat ggtgtatcgg
    11581 gtttaccaat gacctgctct ggtatgtatg gcacatgaat aaatccgaat cgtaggtgag
    11641 ggtaatgctt atcttgtaaa taacctaagt gataaagtgt gtgattacaa acaaatgtac
    11701 ctgcgctatt tgaaagtgct ccaggaagtc cttgattaat aatactttga gtcattgctt
    11761 taactggtaa atttgaaaaa taagctggcg caccgtctaa gtgaatggct tgatcaatag
    11821 gttgaaaatc atcattatct ggaatacgtg catcatcaat attaatggcg acacgttctg
    11881 gggtaatggc atttctacca ccagcttgtc ctatagctag tacaacatca taatgattag
    11941 atgccaacgt tttatttata atattatcta ctttcttaaa agaggttggt agttttaatt
    12001 tatcgattgt atgtgtgcca ataatatctt ctagttgagt cacagcttcc catgagggat
    12061 tgatattttg attgtcaaaa ggcgcaaacc ctgttactaa aatgtgcatt ttaagacacc
    12121 ccgcagtatt aaaatttaat ttagtataac acgcgcataa ttaaaaacat atcaggttat
    12181 gtatgcgcat aaaaaacagc cttgaaaaac ctttataaat cggattttca aagcgtgttt
    12241 tgaaaggtat ttaattaaaa ctaagcatgt tgatgtaaat catcaaagtt tgttaaacgt
    12301 tgttgccact cattatcact aatattattt gcttgaacat aacgattacg ctcatgttta
    12361 gcacattcat aagagcatgc acctaaatat ttagtttcgt tttcttcaga aactaatatt
    12421 tgtttattac attctgggtt agcgcaatta atataacgtt cacatggttt gccatcaaac
    12481 caatccttac caataattgt tttttcaact tggttgatat caacactgat acggtcatca
    12541 aatacgtaca ttttaccgtc ccaatattca ccttttgttt caggatcttt accatatgta
    12601 gcaataccgc catgaagttg agctacatct tcgaaacctt cttttaaaag ccatccagaa
    12661 aatttttcgc atcgaatgcc accagtacag tacgtaacca cttttttatc tgcaaataac
    12721 gctttattct ctttaatcca gtcaggcaaa tctctaaaac gtgtgatatt tggacgaatt
    12781 gcacctcgga aatgacctaa atcaaattca taatcattac gtgcatcaat aatgactgtg
    12841 tcatcatctt caagagcttt tctaaattct acaggtgata aatattggcc agttgtgtgt
    12901 cttggatcga cgtcatcttc taaatctaaa gcaacgattt cttttcgagg acgtacatgc
    12961 attttcttaa aagcatgtcc ttcagcttca tcaattttaa acaccatatc tttgaatcgt
    13021 tcatcggcat gcatatgtgc catatattgt tcggtttctt cttttgtacc agataatgtg
    13081 ccgttaatac cttctgtaga aacaagaatt ctacctttta aatggtgtgc tttacaaaag
    13141 gctaagtgat cctgagcaaa ctgttcaggg tcatcaatcg tcatatattt ataatataaa
    13201 agaacttgat agttcatagg ttaatctcct tctataaaca tgttataagc atgttaattt
    13261 ttaaaataaa gcgtcaatac gcatatttgt ctacgtccat cattttaaca gagttgtatt
    13321 gtcattcaat atttctagct tgaaatcagc cactagaccg ataaaataga tagatgaaac
    13381 cgttatcatt ttatttgata gttatcttgt ttaaaataaa attgatagtt aaaacgaatg
    13441 aaatgaaagg aatgatcatg atgtcacaac aagatttacc tacattattt tatagcggga
    13501 agtccaatag tgctgttcca attatatctg aaagtgaatt acaaacaatt acagctgaac
    13561 catggcttga aatttccaaa aaaggattgc aactagaagg attgaacttt gatcggcagg
    13621 gacaactctt tttattggat gtattcgaag gcaatatttt caaaatcaat cctgaaacga
    13681 aggaaatcaa acgacctttt gtaagtcaca aagcgaatcc tgcagcaatc aaaatacata
    13741 aagatggccg attattcgtt tgttatttag gagattttaa atctacagga ggcatttttg
    13801 cagctacaga aaatggtgac aacttacaag atattattga agatctttca acagcatatt
    13861 gtattgatga catggtattt gattctaaag gtggatttta ttttacagat tttagaggat
    13921 actctaccaa tccactagga ggcgtttatt atgtttcgcc ggactttaga acagtgacgc
    13981 ctatcattca aaatattagc gtagcaaatg gtattgcttt aagtacagat gaaaaagtac
    14041 tatgggtaac agaaacaaca gccaatcgat tacatcgcat tgcacttgaa gatgatggtg
    14101 tgacgataca accatttgga gctaccatac cgtactattt tacaggtcat gaaggaccag
    14161 actcatgttg tattgatagt gacgataatt tatacgtagc aatgtatggt caaggtcgag
    14221 tgttagtttt taataaaagg ggttatccaa taggacaaat attgatacca ggccgagatg
    14281 aagggcatat gttacgttct actcatccgc aatttatacc tggaacaaat caactcatca
    14341 tttgttccaa tgatatagaa atgggcggag gatctatgct ttatacagtt aatggctttg
    14401 caaaaggtca tcaaagtttt cagtttcaat aagacctacc tgaatgaaag gcgtctaaaa
    14461 ttagggtgaa cagttagtgc ttgttacaca tgagttaaac gaaaaatgcc aactatacct
    14521 agggatgata tagttggcat ttcgtatata tattaaaaca atgaggattg tgtaatgaga
    14581 ttattctgag attgagaatt cagctgatgc ttcgaattta acatctttgc ctagcattac
    14641 gccaccagtt tcaagtgctt ggttaaagtt aatgccatat ttttctctat tgattgtacc
    14701 agtaacaata atacctgtta cttgagaacc atccatagga ttcttactta ctccgttgaa
    14761 ttcaacatcg aatgtttctt cgttagtgat gcctttaatt gttaaatcac caacaacttt
    14821 gctttcagat actgattttg tcacaaatgt aattttatca aattcgtcag taccaaagaa
    14881 atcaccagat tttaagtggt tatcacgtgc ttcgttttta gtgttaattg agcttggaat
    14941 aattgttgca gtagctttca aagtactgaa gtcattaata tctccttcaa cagctacatc
    15001 aaattgatca aatgaacctt tcactttaga aaccattaaa tgtttaattt ggaattctaa
    15061 actactgtgt gcaccatcaa aagtaaagtt agtcataata taaaacctcc atatttttat
    15121 ctcttagata tattttttat acttgatata catacaatac cacctaaaaa acaaagatgc
    15181 aagcatttta tttcgaattc gaatattttc tgtgatgtat ttaaagaatg acctataaaa
    15241 caccacacta ttgttatacc ctagtcaatt aattcaaaca taaagaagtg attacaagtc
    15301 cgtgaaattt agttaatgag ataaatctga ctattattat catttttata gtcactacta
    15361 ttgactttgc atcaagtgac tcgtactatt ttaatataat tctgaaaatt caaaatatag
    15421 aacattaaat atgtagataa tatttttttg aagttttaaa aaaaggaggc attttatggg
    15481 aaattatttg gtattgcaat cagattttgg acttagtgat ggcgctgtaa gtgcaatgta
    15541 tggagttgca tatactgtaa gtgatgatat acgtgttgaa aacttaacgc atgatatacc
    15601 accgtatgac atttgggtag cgtcatatcg tttatatcaa actgtaaaat actggcctaa
    15661 aggcacagtt tttgtatcag tggtagaccc gggtgtaggt agtgataggc ggagcattgc
    15721 ttgcttaaca tattcaggtc attacattat tacacctgat aatggttctt taagtcatat
    15781 taaacattac gaaggtatta aaaaagttat cgaaattgat gaagtgaaaa gccgattgcc
    15841 tcattcggaa gaaagtcata cttttcatgg tagagatgtt tatgcataca atggtgcgag
    15901 attagctgca aataagatag cgtttgaacg tctcggtcag gcaattaatg ttgatagtat
    15961 tgaggcatta gaaataagag aagtgacaaa aaatgaggat tctgttacag gaagcattga
    16021 tattttagat atcagatttg ggtcattatg gacaaatatt ccattggcat tcttaaaatc
    16081 cttggaaatt gttcatggta ataatttggt ggtaacaatt tatcatcaag ataagaaagt
    16141 ctatcaaaat attattaaat ttgcgcgatc gtttgcagat gttaatgtgg gtgaacctct
    16201 tgtgtatgtt aactcgctag taaatattgg tgtagcagtc aatcaagact cattttcaga
    16261 tttatatcat attggtactg gaaatgattg gacgattcat ttatctaaag caccatcaat
    16321 ataatgaatt ttttatgtaa ggagtaatga agatgaaaaa acaagatatt tcagttaaaa
    16381 ctgttgttgc aattggtata ggagcagcgg tatttgtaat tttagggcgt tttgttgtaa
    16441 taccaacagg ttttcctaat acgaatatag aaacatcata tgcattttta gcattaatat
    16501 ctgcaatatt tggacctttt gctggtttaa tgactggatt agttggacat gctattaaag
    16561 attttacgac atacggtagt gcctggtgga gttgggttat ttgctcggga attataggct
    16621 gtttatatgg ctggattgga ctaaaattaa atctttcttc aggccgattt tctaggaaat
    16681 cgatggttta ttttaatatc ggtcaaatta ttgcgaatat tatttgctgg gcacttattg
    16741 caccaacatt agatattttg atttataacg aaccagctaa caaggtttat acacaaggtg
    16801 ttatctctgc agtattaaat attatttcag ttggcattat tgggacaata ttattaaaag
    16861 catatgcttc atctcaaatt aaaaaaggta gtttacgtaa ggaatagtta ttttgttgaa
    16921 tcagatatgt aaatgaatat agaaaggtaa tgatatatca tgactgaacc aattatctcg
    16981 ttcaaagact ttagttttca atatcatagt caagcaacac ctacattaca gaatataaat
    17041 gttgatattt atccaggaga aaaagtatta gtagttggtg cttcgggtag tggtaaatcg
    17101 acttttgcaa attgcataaa cggattaatt ccatttaaaa ctaaaggtaa cataactggg
    17161 gaactatata taaataatca agatgcaacc gttagttgtt tacatgatag atctaatgtt
    17221 gttggtacag ttttaaaaga tacagatgga cagttcatag gcttaacagc agctgaagat
    17281 atggcctttt tattagaaaa taattgtgtt gaacaagatg atatgaagaa aaatgtaagt
    17341 tattgggctg aaaaagttgg catgatagaa catttaaatc accgaccgca agatttatct
    17401 ggaggtcaaa aacaacgcgt ttcattaggt ggtatattaa tccatcgtac gcctatttta
    17461 atattggatg agccactggc caatttagat cctgcgacag gacatgaaac gctgagattg
    17521 ttaaacaata ttcatgaaga aacaaagtca acgatgatta ttgtcgaaca tcgattagaa
    17581 gaatcattag atgatacgtt tgatagagtt cttcttttta aagatggaaa aatcatcgct
    17641 aatacaacgc ctagtgattt gctaaaatca tctaagctta aagaagcagg catacgtgaa
    17701 ccattatact gtacagcgct taaatatgca gaagtagatg tcgaatccat tgataactta
    17761 gcgaatttac gtgacgtttg tatgagtgaa catgttaaat ttaaagtgaa aaaatggata
    17821 gatgaaacat ctgcaaataa tgataataag tacaaatctg aaccgctatt agaattaaat
    17881 gaggtatgtg ttcaatatag tgactatagt aatagtgtac ttaataatgt tcaattaaat
    17941 gtttatagaa gagaaatgct tagtatagtt ggtcacaatg gtgccggcaa atcaacactt
    18001 gctaaagcaa tttgtggatt tttagatata acaggtaata tccaattttg taatagggga
    18061 tttaatcaat tgtcaattag cgaacgatct gaattcgtag gttatgtgat gcaaaatcca
    18121 aatcatatga tttctgaaaa aatgatttac gatgaagtag cattagggtt aagagcacga
    18181 ggtatgaaag aatcagatat aaagatacga gtagagaatg tgctcaaaat atgcggactt
    18241 tatgcatttc ggaattggcc aattgcagct ttaagctatg gtcaaaaaaa gagggtcact
    18301 atagcatctg ttttagtctt aaatccggaa ataatcatat tggatgaacc gactgctggt
    18361 caagatttct atcattataa tgagataatg tcatttttaa ttgaactaaa cagacagggg
    18421 aagacgatta ttatgattac gcatgatatg catttattgt ctgagtatag ttcaagaaca
    18481 gttgtattat caaaaggtca agtcgttgct gataccacgc cagtattggt tttaaatgat
    18541 aaaaaaatct gtgagattgc atcattgaga caaacatcgc tatttgaaat ggccgaatat
    18601 atagggatta gcgagccaca gaaattagta caattattta ttaaccatga taggaaggtg
    18661 agacgccaat gaatcaatat aatactatag gttttcaccc gggaaatagt cgtattcatc
    18721 aattaaatgc gactgttaaa cttttattct tattagttgt ttctatttct gcaatggtga
    18781 cttatgacac aagatattta attttaatta gtgcttcatc tattttattg gtcaaatatg
    18841 ctcatattga atggaaacaa gttcgctttg ttgttaaatt cattctgttt ttcacaatat
    18901 taaatattat tgccgtgtac atatttgacc ctgaatatgg tgtgaagatt tataatcagc
    18961 gtacagagtt agtcaatggt attggtcgat ttacgctaac atcacaggaa ttattctatc
    19021 tttttaatct aatattaaaa tatattagta cagttccttt agcgttaata tttttattca
    19081 caacgaatcc gagtcatttt gctgcaagtt taaatcagct aggtgtgaat tataaaatca
    19141 gttacgcagt ctcactagca ttaaggtata ttccagatat tcaagaaaca tattttaata
    19201 tttcacaagc gcaacaagca agaggatatg ataattcgaa aaaagcaaaa tttactagcc
    19261 gtgttaaagg tatcaaacgc attgtgttac ctttaatatt ttctagtatc gaaagaattg
    19321 acactattag tactgctatg gagttaagac aattcggaca gtataaaagg agaacctggt
    19381 acgtcaaaaa acaattaaaa aaagatgatt atgttgtttt gtgtttgacg ttaatacttc
    19441 tgatgttagt agttacatta ttctttttaa ataatagtcg atatttcaac ccgtggcatt
    19501 agtattcata taaatagtct ttaaatagaa ataggaggga gacatttaat gataaatact
    19561 gaaagattaa atttaatgat tccaagttcc tcgcatttaa ttgaacttta taatatttgt
    19621 agtcatccac aagcaaatat atacactccc aaaggtttac ataattccaa attagacaca
    19681 caacggtgga ttgaaaaatg gcgaaaccat tggcaacaat atcaatttgg ttactttgta
    19741 ttggtaaaaa aaatagattg tagtgttatt ggtatttgtg gatatgaata tcgacaatta
    19801 aagcaagaaa cagtattaaa tttattttat aaattacatc caagttttga aggacaaggg
    19861 tacgcatgtg aggctattac agcaatcaca aattttgtga attatatcga tcaagaaaca
    19921 gtaaaagtta tcaggacaaa taagtgtaac caacgttcaa taaatttagc agaaaggctt
    19981 aaattcaagc gagacgatac tatggacgac attatcaatc aaggagatat tgtgttttat
    20041 aaataaaata ctatgacatt atctaaaaaa taaaattaaa atcccaacag tttcttaaaa
    20101 agttatgagc cacataataa aaatgttata attgtgcata ataaaacata gggagaatgt
    20161 ttaatgcata aaaaatattt attgctattt tttctgttat tatcagttgc attgttatat
    20221 agctgttcca agcaaaatga gaaagctcaa aataaatctg ttaatccaaa tgagtatgca
    20281 aagcataaat cgaaaatgga tgaacattgg gaaacatatc acggccaagt ttatcatgtg
    20341 ttttatcacc cattaattac agacccaaaa gttgcgttta caggagatac acaccaagct
    20401 aagggcaaca atgattggat gattactgta agtgaattta aaaaatcatt gaacgaatta
    20461 tataaaaata attacatcat cgttaatcca catgatgtat acgacttatc atctcatcat
    20521 gtaaaagcca aaaaaataaa attgccgaaa ggtaaaaagc cactaatcat ctcaattgat
    20581 gatatgaatt actatagtta catgagacac catggttatg ctgaccgttt agtactcgat
    20641 aaaaataaac atgttgtttc tgaaactaca gacaaaaaag gccatgctac ttattctgat
    20701 gacaatgata tcgtgccaat tttaaataaa tttgtaaaag agcatccaga tttttcttta
    20761 aatggtgaaa aaggtgttgt tgctttaacc ggttatgagg gcgtattagg ttatcgcacg
    20821 aatgaattaa cgagtaaaga ttatacaaag aataaaaagg cagctgagga agttgttcgc
    20881 gcaatgaaac gggatggatg gtcatttgcg agtcattcat atggtcacat taattttgaa
    20941 aaaacatctt tagaaggtat taaacgtgat actaaaagat ggaaagatga agttgaacca
    21001 attgtaggta aaacagatat gtttgtattt ccacatggcg cacaggatag acatacacaa
    21061 gcgtatgatt atttggtaga tgaagcagag tttaagttta ttgcaggtgt cggtccaaac
    21121 aattttacag atatatcagc aacgaacgtt taccaagata gggttgcgat tgatggtcta
    21181 aacttatttg aatttaaata taaactcaaa ccgtttttta atcctgaaaa tgtatatagt
    21241 aaacaagata gacgttactt taaagggaat cgggattatg aagaatagtt aaatttaata
    21301 aaaagataga aagaaaataa ctttacataa taaggcaagg acaatatgtg caagaagtac
    21361 aacttcaata taaaaatctt ggttaagcat ttttataggc acaaatgtct tgcctttttt
    21421 attttaaaaa attttaaaat caatacaccg aattttctga aaaaggttgt aatgtattgc
    21481 taaaaatgat aagttattaa tcatattaca ctttagcgtg gtagagaagt aaagcaaaca
    21541 acgaaaatac caagtgattt taaatacagt attgaaagta aagggtggtc gagtgaataa
    21601 ttcagaacaa ttaattgcgt taaaagaatc ggaaacagca tttttaaaat attttaataa
    21661 agcggattat gagttagttg attttagtgt ggttgaaaag ttggactgga aacaattgaa
    21721 ccatgaggat ttgcagcaga tgggtgagcg aaatttttgg caacatgagc atcaaattta
    21781 tgcacttcga aatgatttta cagatcaatt attgcgttat tactcaatgt atccaactgc
    21841 agcaacaaaa gtggcataca caggtttaat cattcggaat aatgaagcgg ctgtacaagt
    21901 tggtttggaa aactatgcac catcattagc gaatgtgcaa cagagcttaa agctctttat
    21961 tcagtttatt caacagcagt tgagagacaa cgtgcatttt gttgtattgg gacattatca
    22021 attgcttgat gccttactcg ataaatcgtt acaaacacca gacatactat caatgattga
    22081 ggaacgtaat ttatcgggac tggttacata tttgtcgact gaacatccaa tcgtgcaaat
    22141 cttgaaagag aatacgcaac aacaattaaa tgtattggaa cactatattc caaatgacca
    22201 tccagcactc gttgaattaa aaatatggga acgttggtta cataaacaag gttacaaaga
    22261 catccattta gatattactg cgcagccacc tagatcctat tacacaggtt tatttattca
    22321 atgtcatttt gctgaaaatg aatctcgagt tttaacaggt ggttattaca aaggaagcat
    22381 cgaagggttt ggattaggat taacacttta agtaagggag tatgcacaat gttaagaatc
    22441 gccatagcca aaggacgtct aatggatagt ttaattaact atttagatgt aattgaatat
    22501 acgacattat cagaaacatt aaaaaataga gaacgccaat tattattaag tgtagataat
    22561 attgaatgca ttttagtaaa aggaagtgac gtgccaatct atgtggaaca aggaatggca
    22621 gacataggca ttgttggtag cgacatatta gatgagcgcc aatataatgt taataatttg
    22681 ttgaatatgc cttttggagc atgtcatttt gcggttgcag cgaaacctga aacgaccaat
    22741 tatcgtaaaa tcgcaacgag ttatgttcat actgctgaaa catattttaa atcaaaaggt
    22801 attgatgtcg aattgattaa attgaatggc tctgttgaat tggccggtgt tgtagatatg
    22861 gtagacggaa ttgtcgacat cgttcaaaca ggtactacgc taaaagcgaa cggactggtt
    22921 gaaaagcaac atattagtga tatcaatgca agattaataa ctaataaagc agcttatttt
    22981 aaaaaatcac aattaataga gcaatttatt cgctctttgg aggtgtctat tgccaatgct
    23041 taatgcacaa caatttttaa atcaattttc attagaagca ccattagatg agtcattgta
    23101 tccaattatt cgcgatattt gtcaggaagt taaagttcat ggagataagg ctttgaaaat
    23161 gtataatcta acattcgatc atacgaaaac agatcattta gaaattagtc atgaacaaat
    23221 taaagcagca tttgacacat tagatgaaaa aacaaaacaa gcattacaac aaagttatga
    23281 aagaattaaa gcatatcaag aaagtattaa acagacgaat caacagttag aagaatcagt
    23341 ggagtgttat gaaatatacc atccactaga aagtgtcggt atttatgtgc ctggtggtaa
    23401 agcaagttat ccttcaacgg tattaatgac agcgacgtta gcacaagtag caggtgtgga
    23461 aaatattgtc gttgtgacac cacctcaacc taatggtgtg tctcaagaag tattagcagc
    23521 atgctatatt acgcaagttg atcaagtatt tcaagttggt ggtgctcaaa gtattgctgc
    23581 gttaacttat ggcacagaaa cgatacctaa agttgataag attgtaggtc caggcaatca
    23641 atttgttgca tatgccaaga aatatttatt tggacaagta ggtattgacc aaatagctgg
    23701 accaacagaa atagcactta ttattgatga aacagcagat ttagatgcca tcgtatatga
    23761 tgtttttgca caagcagaac atgatgaatt agcacgtaca tatgccatta gtgaagatgc
    23821 gcaagtcctt aaagatttag aatcacgtat tgctaaagca ttgcctaatg tggacagata
    23881 cgacattgtt tctaaaagta tcgctaatca acactacctt atccatgcta gtaattttga
    23941 tgaagcatgc catgtcatga atacaatcgc gcctgaacat gcgtcgattc aaacagtaaa
    24001 tcctcaacca tatattgaaa aagtgaaata tgtgggtgca ttgtttattg gacattattc
    24061 gccagaggtc ataggagatt acgttgcagg tccaagtcat gtattaccta caaatagaac
    24121 agctagattt accaatgggt tatcggtcaa tgatttctta acacggaaca cggtcatcca
    24181 tttatcaaaa gatacgtttg aacaaattgc tgattcagca caacatattg ctcatgttga
    24241 agcattatac aatcaccagc agtctatttt aatacgtcag tcttagggga gtgtaattga
    24301 aatgatttat attgataaaa atgaaagtcc agttacgccg ttggatgaaa aaacaatgac
    24361 gtctattatt agtgcaacgc catataattt atatcctgat gcagcatatg aacaattcaa
    24421 ggaagcttat gctaagtttt acggattatc gcctgaacaa attattgcag gaaatggatc
    24481 tgatgaattg attcaaaagt taatgctgat catgccagaa ggtccggcat taacgctaaa
    24541 tcctgatttt tttatgtatc aagcatatgc ggcacaagta aatcgtgaaa ttgcatttgt
    24601 agatgcagga tcagatttaa cgtttgattt ggaaaccatt ttaacgaaaa tcgatgaagt
    24661 acaaccatca ttttttatta tgagtaatcc acataaccct tcaggcaagc aatttgatac
    24721 ggcattttta acagctattg cagataagat gaaagcatta aacggatact ttgtcattga
    24781 tgaagcatat ttagattatg gtacggcata tgacgtggaa ctggcaccac acatcttaag
    24841 aatgcgtaca ttatcaaagg cgtttggaat tgccggctta agattaggtg tcttaattag
    24901 tactgctgga acgataaagc atattcaaaa aatagaacat ccatatccat taaatgtatt
    24961 tacgctaaat attgcgactt atatttttag acatagagaa gagacaagac aatttttaac
    25021 gatgcaacga cagttagctg agcagttaaa acaaatattt gatacacatg ttgcagataa
    25081 aatgtcagtg ttcccatcaa atgctaattt tgtacttact aaaggctcag cagcgcaaca
    25141 attaggacaa tacgtatatg aacaaggatt taaacctcgc ttttatgatg agccggtgat
    25201 gaagggctat gtaagatact caattgcaac agcatcacag ttaaagcaat tagaagaaat
    25261 tgttaaagaa tggagtgcaa aatatgattt atcaaaaaca acgaaacaca gctgaaacgc
    25321 aactaaatat ttcaatatct gatgatcagt caccatcgca tattaataca ggtgtgggct
    25381 ttttaaatca tatgttaacc ttgtttacat ttcatagcgg tctgtcatta aacattgagg
    25441 cacaaggtga tattgacgta gatgatcacc acgtaactga agatatcggc attgtcattg
    25501 gccaattgtt acttgaaatg attaaagata aaaagcattt cgttcgttat ggaacgatgt
    25561 acattccaat ggatgaaaca ttagcacgtg tcgttgtgga tataagtggg cgcccatacc
    25621 tatcattcaa tgcatcatta agtaaagaaa aagttggtac gtttgatacg gagttagtag
    25681 aagaattttt tagagcggtc gtaatcaatg caagattaac aacgcatatt gatttaattc
    25741 gtggaggtaa tacacaccat gaaattgaag ctatattcaa agcgttttcc cgtgcattag
    25801 gcatagcgct aactgcaact gatgatcagc gtgtgccgtc atcgaaaggt gtgattgaat
    25861 gattgtcatc gttgattatg gattagggaa tattagtaat gtaaaacgcg ctattgaaca
    25921 tttagggtat gaggtggttg tctcaaatac ctcaaaaata atcgatcaag cagaaacaat
    25981 catattgccc ggtgtcggcc attttaaaga tgcgatgtca gagataaaac gattaaatct
    26041 caatgcaata ttggctaaga atactgataa gaagatgatt ggtatttgtt taggcatgca
    26101 attaatgtat gagcatagtg atgaaggcga tgcatctgga ttagggttta tcccaggaaa
    26161 tatttcgcgt atccaaacag aatacccagt gccacactta ggctggaata atttagtgag
    26221 taagcaccct atgttaaatc aagatgttta tttcgtacat tcttatcaag cgccgatgtc
    26281 agaaaatgta attgcatatg cgcagtatgg ggctgatatt ccggcaattg ttcaatttaa
    26341 caattatatt ggtattcaat tccatcctga aaaaagcggt acatatgggt tacaaatttt
    26401 gcgtcaggca atacaagggg gatttataaa tgattgaatt atggccagcg attgatttga
    26461 ttgggtcaac aagtgtgagg ttaacagagg gtaaatatga tagtgaagaa aaaatgtcac
    26521 gctcggctga agaaagtatt gcttactata gtcaatttga atgtgtgaat cgtattcata
    26581 tcgtcgactt gataggtgct aaggcacagc atgcccgaga gtttgattat attaagtcat
    26641 tgaggagatt aacaaccaaa gatattgaag taggtggtgg cattcgtacg aagtcacaaa
    26701 tcatggacta ctttgccgca gggattaatt attgcatagt tggaacgaaa ggtattcaag
    26761 atactgattg gcttaaagag atggcacata catttccagg tcgcatttat ttatctgttg
    26821 atgcctatgg agaagatatt aaagtgaacg gatgggaaga ggacacagag ttaaatttat
    26881 ttagttttgt gagacggtta tcggatatac ctcttggcgg cattatatat actgatattg
    26941 ctaaagatgg caaaatgtcc ggacctaact ttgaattaac tggtcaatta gtaaaggcaa
    27001 cgacgattcc tgtcattgct tccggtggta ttagacatca gcaagatatt caacgattag
    27061 cgtcgctaaa tgttcacgct gctattatag gaaaggctgc acatcaagca tctttttggg
    27121 aggggctaaa atgattaaaa aacgtatcat tccatgttta gatgtcaaag atggtcgtgt
    27181 cgttaaaggg attcaattta aaggattaag ggatattggg aatcctgttg atttagcaat
    27241 gtattacaat gaagcgggtg ctgatgaatt agtattttta gacatctcta agacggaaga
    27301 gggtcatagc ttaatgctag aagtgattga acagacagcg tcacgcttgt ttatccctct
    27361 tactgtaggg ggtgggattc aaagtctcga tgatattacc caattgctaa atcatggtgc
    27421 agataaagta tcattaaatt caagtgcttt aaaaaatcca cagctcatta aacaagcgag
    27481 tgataaattc ggtagacaat gcatctgcat agcaattgat agctattatg atcctgaaag
    27541 aaaagcacat tattgttgta cgactggtgg taaaaaaatg acaaatatta aagtatatga
    27601 ctgggtacag caagtagaac agttaggtgc aggtgagctc ctcgttacaa gtatgggaca
    27661 tgatggtatg aaacaaggct ttgatattga acacctagca aatattaagt ctcttgtaaa
    27721 tattccaatc attgcttctg gtggtggtgg caatgcacaa cactttgtag aattatttga
    27781 tcagacggat gtttctgcag gtttagctgc aagtatatta catgatcgag aaacgacggt
    27841 tcaatctatt aaagaagtga tacggcaagg gggtatagca gtaagatgac caaatataaa
    27901 attgatttta gcaaaggttt agtgccagca attttacaag ataatcaaac aaaacaagta
    27961 ttgatgttgg gttatatgaa ccaagaagct tttgataaaa cgatagaaga tggtgtggta
    28021 tgtttctatt cgcggtcgaa acaacggcta tggacaaaag gtgaaacatc tggtcatacg
    28081 caacgtgtta aagatattca tgtagattgc gacaatgaca ctattttaat tgatgtcata
    28141 ccaaatggac caacatgtca tacaggcagt caaagttgtt tcaacacaga agttccattt
    28201 tcagtgcaaa cattagcgca gacagttcaa gatagtgccc aatccaataa tgaaaagtca
    28261 tatacaaaat atttattaac agaaggtata gaaaagatta caaaaaaata cggtgaagaa
    28321 gcttttgaag tcgtaattga agcaattaaa ggtgacaaaa aagcatttgt aagtgaagta
    28381 gcagatgaac tttatcattt atttgtcttg atgcatgcgc ttggcgtcga tttttcagaa
    28441 attgaggcgg aattagcgcg tagacatcat aagcgcaata actttaaagg tgaacgacaa
    28501 aatatcgaac agtggtaaag caagtatgga ctaagatata aggaaaagga tcatggctta
    28561 tacacttaca aatattgtgg aaaacgtgac attttcaagt ttaaaatacg acaccaacat
    28621 attttaacta tgaatgctgt gatggtacta aagttgcgaa ctcgttatag ataagtagtg
    28681 gataatcaca atacgaaatc aaaaataatt ataaaaagta aattgagcaa ctcaggaata
    28741 gatgtcactg ttaaagatgt cgaaaagtat atgaatcgat ataatgaagt tatgaaggga
    28801 aaaaatggcg aaaaagctaa agagttatgt ttgtcgttac tacctattaa tatcatagtt
    28861 gtctttacat tctttgtatt tatactataa atacaaatat atctagcctg aaatagaaat
    28921 gtcatagcct atttaaaaga caatctccat tagaactaag atatgcatcc cgaaagttag
    28981 actaaaaaac taactttatg ggatgtattt ttatgctaat catcataaat tcgagattaa
    29041 gttaaaggta gttcaagagt aattaaacaa taaattaaaa atagtaggat acttactttg
    29101 agggaagaaa attaactgta tatatttagt ttaggaacaa gtattacggt ttatcctgat
    29161 acaattattg tggatgggat gatattttta ggtttaaaat acgacaccag caaacataat
    29221 aactgtaata gctcataaat ctccccatat agctaatcta aaaaaataat acatcattgg
    29281 aattaagccc caagcatgta aatattaaaa atcaaaaaag atatatg
//
LOCUS       DAKTIP010000011        28893 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAKTIP010000011 DAKTIP010000000
VERSION     DAKTIP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 28893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.011"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            235..873
                     /locus_tag="O0305_000374"
     CDS             235..873
                     /locus_tag="O0305_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCY0273560.1"
                     /translation="MYKINIIRSDSVYNNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQISPKDLRMSDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDRQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTN"
     gene            892..1149
                     /locus_tag="O0305_000375"
     CDS             892..1149
                     /locus_tag="O0305_000375"
                     /inference="COORDINATES: protein motif:HMM:NF021537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273561.1"
                     /translation="MKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYA
                     CGYHLVKYFLQKTNIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            1128..1868
                     /locus_tag="O0305_000376"
     CDS             1128..1868
                     /locus_tag="O0305_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0273562.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(2126..2779)
                     /gene="nreC"
                     /locus_tag="O0305_000377"
     CDS             complement(2126..2779)
                     /gene="nreC"
                     /locus_tag="O0305_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCY0273563.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(2802..3836)
                     /gene="nreB"
                     /locus_tag="O0305_000378"
     CDS             complement(2802..3836)
                     /gene="nreB"
                     /locus_tag="O0305_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCY0273564.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(3860..4312)
                     /gene="nreA"
                     /locus_tag="O0305_000379"
     CDS             complement(3860..4312)
                     /gene="nreA"
                     /locus_tag="O0305_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCY0273565.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(4332..5009)
                     /gene="narI"
                     /locus_tag="O0305_000380"
     CDS             complement(4332..5009)
                     /gene="narI"
                     /locus_tag="O0305_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCY0273566.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEHLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(5002..5577)
                     /gene="narJ"
                     /locus_tag="O0305_000381"
     CDS             complement(5002..5577)
                     /gene="narJ"
                     /locus_tag="O0305_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCY0273567.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(5570..7129)
                     /gene="narH"
                     /locus_tag="O0305_000382"
     CDS             complement(5570..7129)
                     /gene="narH"
                     /locus_tag="O0305_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCY0273568.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(7119..10808)
                     /locus_tag="O0305_000383"
     CDS             complement(7119..10808)
                     /locus_tag="O0305_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCY0273569.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(11117..12094)
                     /gene="cobA"
                     /locus_tag="O0305_000384"
     CDS             complement(11117..12094)
                     /gene="cobA"
                     /locus_tag="O0305_000384"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCY0273570.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVDYTENTPKSYDPMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            complement(12085..12399)
                     /gene="nirD"
                     /locus_tag="O0305_000385"
     CDS             complement(12085..12399)
                     /gene="nirD"
                     /locus_tag="O0305_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCY0273571.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKGKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(12403..14808)
                     /locus_tag="O0305_000386"
     CDS             complement(12403..14808)
                     /locus_tag="O0305_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0273572.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTLDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARRIFEV
                     EKV"
     gene            complement(14874..15605)
                     /locus_tag="O0305_000387"
     CDS             complement(14874..15605)
                     /locus_tag="O0305_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCY0273573.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            complement(15835..16371)
                     /locus_tag="O0305_000388"
     CDS             complement(15835..16371)
                     /locus_tag="O0305_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273574.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            complement(16604..17428)
                     /locus_tag="O0305_000389"
     CDS             complement(16604..17428)
                     /locus_tag="O0305_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0273575.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(17645..17827)
                     /locus_tag="O0305_000390"
     CDS             complement(17645..17827)
                     /locus_tag="O0305_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273576.1"
                     /translation="MVERYIKVLILYIFTTLLSSISVTSKCVPNKVIRFILRTAIGYS
                     VFAYGLHYFSNLKKNK"
     gene            complement(17911..18378)
                     /locus_tag="O0305_000391"
     CDS             complement(17911..18378)
                     /locus_tag="O0305_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0273577.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(18564..20114)
                     /gene="adcA"
                     /locus_tag="O0305_000392"
     CDS             complement(18564..20114)
                     /gene="adcA"
                     /locus_tag="O0305_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCY0273578.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADCYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
     gene            complement(20303..20374)
                     /locus_tag="O0305_000393"
                     /pseudo
     CDS             complement(20303..20374)
                     /locus_tag="O0305_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20409..20675)
                     /locus_tag="O0305_000394"
     CDS             complement(20409..20675)
                     /locus_tag="O0305_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0273579.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(20675..20926)
                     /locus_tag="O0305_000395"
     CDS             complement(20675..20926)
                     /locus_tag="O0305_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0273580.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSVMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(21091..21192)
                     /locus_tag="O0305_000396"
     CDS             complement(21091..21192)
                     /locus_tag="O0305_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273581.1"
                     /translation="MLRSVSDTNLLGQYEKAISISDMIEIVFLMYEV"
     gene            complement(21199..21798)
                     /locus_tag="O0305_000397"
     CDS             complement(21199..21798)
                     /locus_tag="O0305_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCY0273582.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVVYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(21817..22176)
                     /locus_tag="O0305_000398"
     CDS             complement(21817..22176)
                     /locus_tag="O0305_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0273583.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSR
                     RYYQQHDADYHEENMTN"
     gene            22431..23681
                     /gene="fmhA"
                     /locus_tag="O0305_000399"
     CDS             22431..23681
                     /gene="fmhA"
                     /locus_tag="O0305_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCY0273584.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKHNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(23778..24509)
                     /locus_tag="O0305_000400"
     CDS             complement(23778..24509)
                     /locus_tag="O0305_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0273585.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGMIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(24506..25225)
                     /locus_tag="O0305_000401"
     CDS             complement(24506..25225)
                     /locus_tag="O0305_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCY0273586.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(25206..25985)
                     /locus_tag="O0305_000402"
     CDS             complement(25206..25985)
                     /locus_tag="O0305_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCY0273587.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(26106..27545)
                     /gene="mdeA"
                     /locus_tag="O0305_000403"
     CDS             complement(26106..27545)
                     /gene="mdeA"
                     /locus_tag="O0305_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCY0273588.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRNLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            27719..27823
                     /locus_tag="O0305_000404"
     CDS             27719..27823
                     /locus_tag="O0305_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCY0273589.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(27977..28663)
                     /locus_tag="O0305_000405"
     CDS             complement(27977..28663)
                     /locus_tag="O0305_000405"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCY0273590.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
BASE COUNT         9043 a         5630 c         4094 g        10126 t
ORIGIN      
        1 tatattttat tatgattgtg aaaaagtgaa cataaggatt ttcccttatt tatgaagtga
       61 tttaccctat acaatgtccc tttaagatgc ttttagacac ttattcgcca tcattttaat
      121 catattattt aggaacactc acatactttt cttaattttt ttcaattagt tacaattact
      181 gcttgaaaat cacgttacgt tatcctttat gctgacaaca taaggaggtt tatcatgtat
      241 aaaatcaata ttattagatc agattccgtc tataataata tactcaaggc accaataaac
      301 gaccgtgatt caattttcac taaagaaata ttagtacctt ttaaaaagaa atttgaggta
      361 caacatatgc ctatttataa tgatgacaaa cagacaatga gtgcgattca atttttagat
      421 gcctttcaaa tttctcccaa agatttacgc atgtctgacc aaatgtcaat acaatacctg
      481 aataatgatt tctggagtaa ttgcgaaaaa tatttaaaag ttgctataga tcaattttca
      541 aattatagta tctcatctca agtttctaac tatcatttca cagtattact tggagatcgc
      601 caaaaaccac ttatgtatct aaataaaaat cgcggtggtg atggtggcat accaggttat
      661 attatgattt atttagtgcc gagtacaagt acaattaatt ctatgaaaag cttaattgca
      721 catgaagtaa atcataatat gcgctatcaa tatattgatt gggatggcgg aagtttgatt
      781 gaattgatta ttgcagaagg attagctgaa aactatgtag aatcattgta tggtaaagcg
      841 catattggac cgtgggtaac aaatactaat tgaagtcgtg ataacgtaaa aattaaaaat
      901 actatttata atcatttaca tttaaagcat atatttgaat cgatgcctta tctctatggt
      961 gatgatatta ataaacttca aggtaggcct atcgttggct tatctcatgc tgccgggtat
     1021 gcatgtggct atcacttggt aaaatacttt ttacaaaaaa caaacatacc tattgaagtt
     1081 gctacaacac ttccagcaca aaaaataata aatgaggtga ctgaattttg gcatacacat
     1141 acactttaaa agatattatt gaaattacag gtgtaactaa aagaacttta cattattacg
     1201 atgaaatagg attattagtt ccagataaaa atgataaaaa ttatcgcgtt tataaacagc
     1261 aagacttaga aaaattacaa aagattttaa tactcaagtc ttttgatttt gatatcgcta
     1321 aaataaaaca atacatttcg tatgataatg aacaattgcg aaagttatta tcagagcaaa
     1381 taagcaagtt agataaaaag atttctgact tacaattaat tagacgctct gtatgtgaat
     1441 ttattaaagg actctctcta atagatacca gcattttaaa caagacacta cagtcacaat
     1501 atgataaaga agcatctata aaatatggtc atacgaaagc atatcaatca tttattagac
     1561 gtaaagacag cttacaatcg caggatatca gacataaatt gacaactatc ttcaataaat
     1621 ttaatcatat gtctttgagt cattatccaa tccaagattg tagtgatctc gtatttgagt
     1681 ggaaggcatt tatgaacact atcgctgatt ttgatgatga aacattatgc tgtattgcta
     1741 aaacatatga agatgatacg cgtttcaaag attactttaa ttcatatgat aatcaaaatt
     1801 tagcatcata catttcagaa gctgttaatt attttttgag caatgtgaat aagagcgaca
     1861 atttttaatc ctcatacaga tgctacaaca ctgaatacac caactaaaat aattgttcaa
     1921 agtagaaaac taatcattta ttctctaaat caataactat attaaaagtt atacctttgc
     1981 aaagcgaatt agtataggtt accgaaagga gaaaggatta ggttccattc gatttataaa
     2041 aaaatatatt tcgaaacata caaaagcgcc agaagataat catttcctct cgcgctttta
     2101 tactgaatta tattaatttc aaactctaaa actctaataa tttctttttc aatgcatatt
     2161 caactagctc aggtttactc tttaagccaa gctttgtcat aatatgcgtc ttatgtgctt
     2221 ctactgtttt cacagataca aataatttct ctgcaatttc tttattcccg taacctttgg
     2281 caataagtgg caatatttct agttctcgtt ttgataagat tttaaaagga tctgttgtgt
     2341 ttgcagtgtc ctgatttgaa ttagatacaa attcattcac taaagatgtt gtaagtttca
     2401 tatctacata tgtttcacct ttatatacag ttcgaatagc taacaataat tgttcatcag
     2461 gtgcattttt caatatgtaa cctttcgcac cattacgcaa cacatggaac aaatactcct
     2521 catcatcaaa cattgttaat attagtattt tagtttcagg aaaactgtca gcaattttac
     2581 tcgtagcgat aagacctgac tcacctggtg gcatacttaa atccattagt aacacatcag
     2641 gtttatattc cattactttt tggtaagctt cgacgccatc tgcagccgtt gcaacaactt
     2701 ccatatcatt ttgataattt aaaatcatag agaaccccgt acggacaaca gcgtgatcat
     2761 cggcaatgac tattttcaat tttattcccc caatgtatgt ttcaaattgg aatgttcaat
     2821 gtaacattgg taccctcacc aattttcgtt tcaatattga cgctaccgct gactaactca
     2881 gctcgctcat tcattccata taaaccgagt ccagaacctt taggcttaga acttggatca
     2941 aaaccatttc ccgcatctat cacttctgct accaaatggc gcccagtttg acggatacct
     3001 acatttattt catttacatc agcgtatttc aacgcattta aaatagcttc ttgcactact
     3061 cgataaacaa ccgtttcaat atcactatca aagcgagtat ttttaatatt tgatgtatat
     3121 atgattttta ttccataatt ttcttcaaac tgtttaaaat atgatttaaa agctgcttca
     3181 aggcctagat catccaaaga agcgggtctt aattcaaccg acatattacg tatatcatca
     3241 attaatttag cgacaatata ttcaatattt tctgcgtctt ccaaaagctt agttgtatct
     3301 tcttgatatt ttaataatct caattgaaca tctacattga gcatttcttg aatcacacta
     3361 tcatgtaact ctctagaaat tcgctttctt tcattttctt gggctgagat tgttttacgc
     3421 atcatacgtt gttgatgcaa tttctcttgc tgttcaattt gtgatgaaac attttgaagc
     3481 gtaaatgcat gaattcccct gtcttgatca atcaactgat atgttgctgt aaatggcatc
     3541 actttttgat ctttcgtctt cataaatact tggaaattcg tagcttgtac ttgcatcgat
     3601 tctaagaaac agtctttaca tgattgtaca tcataggcat ttgtataccc ttcacatctg
     3661 tgacaaatcg cattcgccac agcactgtaa ttatcttctt cagataatat atctttagca
     3721 gcatcattca ttgcaataat tttaccgtta tcatcagcaa aaactatctt ttcgattgaa
     3781 tgctcataat attttttcaa taaagtatct aactgtatac tgtcctcatt aatcatgact
     3841 tacaccctaa ttcatctcat tatttatcat cattgaaaat accaaactta cgttgaatat
     3901 catcattatc aaatattttt ggtaaaggac gaccatctct ttgaccaaat aatagtacgc
     3961 catacacttg attcttatac caaagcggca ctgctaaaac tgctgttaat gattcgctca
     4021 ataaaattgg atagtcaatc ttttcttcag gccctaaagc taaaccaaca ttggctatta
     4081 ccatacgctt tcctgttttc ataacagttc cagctaatcc acgacctttt cttaaaataa
     4141 tcaatttaaa tcgattattt ttattacctg aaacatagtg ccattttatt ggagatgatg
     4201 gtttgttaga ttcatagaaa gcgattgccg caaaatcata accctcttct ttgcgtattt
     4261 tatctaatgt ctcttgaaat ctacgatctt caattattgc ttctggtgtc aaatcctttc
     4321 acctcttatg cttacacttt attcttacgg taaataatat atctgcgatt tatatatgtc
     4381 aaaggtacac tccaaacatg caccaaacgt gtaaatggcc aacaagccat aatagtgaaa
     4441 cctaacaata tatgcatttt aaatgcaatc ggcacaccac tcatcaatga cgcatctggt
     4501 tttaacataa ataattgtct aaaccaaatt gataatgaag ttctgtagtt aaagtctgga
     4561 tgttgtatat ttgttactaa tgttgcgtaa catcccataa atacgataag taataataag
     4621 aaatttacaa atatatccga cgctgaactt aatcttcgaa tacttttcgt agtaacacgt
     4681 ctcgctgtta ataaaaacat ccctatcaaa gttattatac caaagatgct accaatataa
     4741 acagcgccta tatgatataa atgctcagac acacccactg catccatcca tggtttcggt
     4801 attaacaatc caactacgtg tccaaaaaac actggaataa tacctaagtg aaataataaa
     4861 cttccccaca tcaacctttt tctttctatt aattcactag atttagctgt ccaagaaaat
     4921 ttatcataac gataacgtgc aatatgacct gcgacaaaga caactaaaca taaatacgga
     4981 aatataaccc ataaaaactg attaagcatg atgtttcact ccttttggtg atgtcaaaca
     5041 taatttcaat gtttttctaa gtgcttgaat cacataggca tatggattgt tatcttcacc
     5101 aagtgcattc gccatcacat atgttccatc ctcaataatc ataatgatta attgaatatt
     5161 ctcttcagct cttggatcat ttcgccattc tgccacttgc aaaaattgaa gcatcaacgg
     5221 tagataatca gaaagttcat tatctaccat ttctagtcca aacatttcat ataatacctt
     5281 taatttagct aacatttgcc cacgttcttt ttgcgtatca aatttgttat acgtcatata
     5341 taatggtgct tttttcgtaa aatcaaatgt atctgtataa atcgctttga tttctgataa
     5401 tgaaaatgtc atcattacat ttctaaatgc tactaaatct tcatatgctg gatgatcttt
     5461 cgaaattgtt tcttcaaaag tttttggatg aaaagttaat ttttctggaa aacataactg
     5521 ttgtgccata tatccaaaac tttcttgata ttttttaaaa ttatcgaaat taatcacgga
     5581 aaatccctcc atagaaattc tcattataaa tttcttgacc agttttccct gaacctactg
     5641 caacgccaca gccttcacag ttatctccaa aatgctcgcc gccgtaattg tatcctgtac
     5701 taccttgtgc gtgatacgta tctaaatagg tttctttgtg tgatgttgga ataacaaatc
     5761 gatcttcata tttggctagt cctaataaac gatacatgtc tttagtttgg cgctcggtta
     5821 tacctaatcg ctctaatcga gacgtgtcaa atggctgttg agtaacttga gatctcatat
     5881 aacttctcat cattgccata cgttgtaggg ctccttttac tggctctgta tctcctgcag
     5941 tgaaaatatt agctaagtat tcaataggta aacgcatttc ttcaatggct gggaaaatcg
     6001 catctggatt ttgagttgta tttttacctt caaaatagct cataattggg ctaagtggtg
     6061 ggcaatacca aaccatcggc atcgttctaa attcaggatg taacggaaat gcaagtttat
     6121 attcaattgc taacttataa attggagagt tttgtgcagc ttcaatccaa tcgtaaccaa
     6181 taccatcttt ttcagcttga gcaatgactt cttcgtcaaa tgggtttaag aatatatcta
     6241 attgtttttc atataaatct ttctcgtcta ctgctgaagc tgcttcatga actcgatctg
     6301 catcatataa taaaacacct aagtaacgca tacgtcctgt acaagtttca gagcataccg
     6361 taggcatacc cgcctcgatt ctcgggaaac agaaagtaca cttttcagct ttgttcgttt
     6421 tccaattgaa gtaaactttc ttatatggac aacctgtcat acagtaacgc catccacgac
     6481 atgcgtcttg gtcaactaat acaatgccat cttcatcacg tttatacata gcacctgaag
     6541 gacacgatgc aacgcaactt ggattcaagc aatgttcaca taaacgtggt aaatacatca
     6601 taaaagtttc gtcaaattgg aatttaatat cttcttctat tttttggatg ttaggatctt
     6661 ttggacctgt aacatgacca cctgctaagt catcttccca gttaggtccc cattcaattt
     6721 caatgttatc ccccgtaatt tctgaatacg ctctagcaac tggcgaatgc ttccctgatt
     6781 tcgcagttgt taaatgttca taattatagt tccatggctc ataataatct ttaattaatg
     6841 gcatatctgg gttataaaaa attttaccta aagcaatttt tgaaattcta cttccagatt
     6901 ttaattcaag tttcccttta cgatttagta cccaaccacc tttgtagtgt tcttggtctt
     6961 cccaacgttt cggataccct acacctggct tcgtttctac gttgttgaac cacatgtact
     7021 cagcacctgg acgatttgtc caagtgtttt tacatgtcac actacacgta tggcatccta
     7081 tgcatttatc taaatttaat accatcgcaa cttgcgcttt aatcttcaag ccaattaacc
     7141 tccttcatct ttctaactgc tacatataaa tccctttggt tcccaattgg tccataataa
     7201 ttaaagtgat aactaatttg tgcgtatcct ccgactagtt gtgttggttt caaatggatt
     7261 ctagtcggcg cgttgtgtga accaccacgt gtatctgtaa tttctgaccc aggcgtttga
     7321 atatgtttat cttgtgcatg atacataaac attgtacctt taggcatacg atgcgaaata
     7381 actgctcttg ccgttacaac accattacgg ttatacactt ctagccaatc attatcttgg
     7441 atatcgtgtt tttcagcatc ttcatttgat atccaaaccg ttggaccacc tctaaatagt
     7501 gtcaacatat gcttattatc ttgatacatt gagtgtatat tccattttcc atgaggcgtt
     7561 aaataacgca gtaccaaagc atctgtacca cctttaattt tcttatctct attcccaaat
     7621 accattggcg gcaatgtcgg tttatatact ggtaagctct ccccaaattg ttggaaaact
     7681 tcgtgatcca cataataact ttgacgtcct gttaatgttc taaaaggtac tagacgttct
     7741 atattcgttg taaatggtga atatcgtcga ccttgtttat ttgaacctgg gaatactgct
     7801 gtcggtatta cttctcgtgg ttgtgaagtt atatttaaaa acgaaatttt ctcagcagcg
     7861 cgttcgctag aaatatcttt taacggcatt ccagtttgtt cttcgagatc ttcatatgat
     7921 ttttgtgata atttaccatt cgtagcagat gaaatactta gtattgcatc agctacatta
     7981 cgtgctgtat caatacgtgg acgattcgct ctcacagaat catcatttgt atcactccac
     8041 gtacctaaca tactttttaa ttcttcatat tgttcactga caccgaaact tacaccatgt
     8101 gctccaactt tccctttttc aagtacagga ccaagcgtga catatttgtc gtaaatttta
     8161 gtgtagtcgc gttctacaat tgcaaagtta ggcattgtac gtccaggtac cgcttcaatt
     8221 tcacccttcg accaatcttt cactacgccg tatggtgttg aaatttcttg ctttgtatca
     8281 tgactaagtg gagttgtcac aacatcttta aacgttccag gtaaatagtc ttttgccatt
     8341 tctgaaaatg cttttgccaa cgttttataa atatcccagt ctgaacgcga ttcccataac
     8401 ggatcaatgg caggattgaa aggatgtaca tatggatgca tatccgttga tgataaatca
     8461 tgtttttcat accaagtcgc tgccggcaaa acaatgtcag aatataacgg tgttgccgtc
     8521 attctgaagt ctaaagagac cactaaatct aacttacctg ttgtttcttc acgccacgta
     8581 atttcttctg gcttttcatc ttcatttggt gtagctaata accctgattt tgtgccaagt
     8641 aaatgcttca taaagtattc ttgacctttt gcagaacttg aaattaagtt tgaacgccat
     8701 ataaataatg attttggatg attctttttc aaatcaggat cttctattgc aaattgtgtt
     8761 tgttttgatt tcacttcatc aattgcacgt tgcaaaatcg cttcatttga atctatacct
     8821 tcatctttag cttcttctgc aaacaacaaa ctatttttat taaattgtgg atatgatggt
     8881 aaccaaccaa gtctagctgc taaaacatta taatcagctg gatgttgatg ctttaactcc
     8941 tctgttttag ctaatggaga ttttaaacga tctacatttg actcttcata tttccattgg
     9001 tctgttgcaa aatagaacca acttgttccg ttttgcaaac gtggtggtcc ttgccagtct
     9061 ttcgcaaatg cgacagtact ccatccttca atcggacgac atttttcttg tcccacatag
     9121 tgagcccaac cgccaccgtt cacaccttga cagccacata acataactaa gtttaagatt
     9181 gaacgataaa tcgtatctga gttaaaccaa tggttaatac ccgcacccat gataatcatt
     9241 gaacgccctt cagtatcgat agcgttttgc gcaaattctt tcgctacttg aatgacaaca
     9301 ctttgtttta cgcctgaaat ggcttcttgc caagcaggtg tatattttga ttctgcatcg
     9361 tcgtatcctt ttgattctaa tttatgatca aaacgacgca cgccatattg acttgccatt
     9421 aagtcaaaaa ttgtagcaat acggactttg tcaccatttg ctaaagtgac ttgtcgagtt
     9481 ggaattggac gattgaatat cccatctcca tcactatcaa agtatgggaa ttgaattgtt
     9541 tctaattcgt atccaccttc tgtcattgat aatgtagggt taattttaga accatcttct
     9601 gtttctagtt ttaagttcca cttcttacct tcttcccaac gttgacccat tgtgccatta
     9661 ggtactacta aactatcgct gattgcatca tgaataactg gcttccattc gccttgctct
     9721 gttgtttgac ctaagtcact cgctcttaaa aatcgacccg ctttatatcc attttcatct
     9781 tcatccagca tgataagaaa cggcatatct gtatattgtt tagcgtaatt tataaagcgt
     9841 tcattaggtt gattaacata atgttcttgt aaaataacat gcgtcattgc ttgtgcaatt
     9901 gcagcatctg aaccaggatt cggtgctagc cagttatctg caaatttcac attttctgcg
     9961 taatctggtg ctactgaaat gacttttgta cctttatagc ggacttcagt cataaaatgt
    10021 gcatccggag tacgtgttaa aggtacatta gagccccaca taataatgta tgatgcgtta
    10081 taccagtcac ttgattcagg cacatctgtt tgctctcccc aaatttgtgg agaggcaggt
    10141 ggtaaatctg cataccagtc ataaaaacta agcatttcac caccaagcaa attgatgaat
    10201 cgagcacctg ctgcataact aatcattgac atcgctggaa taggtgtaaa tcctgcgatt
    10261 cgatctggac catatttttt tattgtatac agtaattgtg ctgcgattat ctctgtaacg
    10321 tctttccaat ttgaacgcac gtgccctccc atacctcggg cttgcttata ttgtttggct
    10381 ttgtcttcat tttcaacaat agacgcccat gcagcaacgc gattaccatt gttttcttct
    10441 aatgcttcag tccataaatc ccagagtttt ccacgaatat atggatattt gattcgaagc
    10501 ggactgtatt cataccaaga gaatgacgca cctcgtggac atcctctcgg ttcatattca
    10561 ggcatatccg gaccacaact tggatagtca gtttgttgat tttcccaggt aatcacacca
    10621 tttttcacaa atactttcca agaacatgag cctgtacagt taacaccatg tgttgttctt
    10681 acttctttat cgtggctcca acgttctctg tacatttttt cccattctct acttttactt
    10741 tctaggatcg accaattccc attaaatttt tctgttggct taaagaaatt caatccaaat
    10801 tttcccatat ttatatcctc ctacgtataa aaatacgatg tgtagatgtc gtgtttttaa
    10861 atactttaaa atgcccaaga ctattgcttt aattagattg tacatttttt cacaaacata
    10921 aaatattagg gaatcaccta attacttaag gaatttccct atcaataacg ggatttcatt
    10981 gaaataatac acaatcatgt atggtcatgc ttattgccaa tctaaatcgt tcaaatttgg
    11041 cacaacgaca aataaggctt caacacgaat atattctctc ggttgaaacc ttacttattc
    11101 atttattttt tataaattag tgacataaca ctgtattagc atctgcacga tcggttgaaa
    11161 tatatgttac attttcttgc tgcttaataa atgcatcata gtaatcatat tgcgacgaat
    11221 gatatgtgcc attcgatgta tcatttgggt ttagcaaaca gccataacct tcgtcatata
    11281 aatgttcaca gagcataagg gcgtcatgtt tagaaccact tactacataa aattgcttca
    11341 taggatcata tgatttagga gtgttttcag tataatcaac aacttcccct ataatacata
    11401 tacctggttt cgcctcaatt gaatagtgtt gcaattttga aataatatta cttaaacgcc
    11461 ccttaacaac aaactcgtta aaacacgatg cttgaaagac aatcgctatc gggtaatcaa
    11521 tatctgtgta ttgttgtatc tgtgtgataa ttttccctaa acgttttacc cccatataaa
    11581 ttgctaacgt gccaccattc actaaggaat tgacatccac ttcattttct tctgaatctt
    11641 taaagtgacc tgtagaaaat gtcacacttt tagcaactgt acgcattgtc aaacctgtct
    11701 gcatagtagc aactgctgcg ctcgctgatg tcacccctgg tacaatttca aacgcaatat
    11761 gatgttcatt tagtgtgtcg acttcttctt gcacacgacc aaatatcgct ggatcgccac
    11821 ctttaagtct aacaaccttg ttatatcgac gcgctgcttc cacgatacag tcatttattt
    11881 tttcttgctg aatatgtttt gcatacggct ttttaccaac atcgataatt tcagtagtca
    11941 aattcgcata ttgtaaaatt aacggattca ctaatcgatc atatagaatg acatccgctt
    12001 cacgtattaa acgctcagcc tttttcgtca aataattcgg attacctgga cccgcaccta
    12061 tcaagtaaac cttgccatat tcctctacag acatatatat acgttcccgt ctgtaacttc
    12121 tacctcataa acatctacac aaccttcatc aggttcttga acaatacctg tatttaaatc
    12181 aattttttga tcgtggagcg ggcaaaatac atattcccca ctcactgtcc cttcagacaa
    12241 tggtccttgt ttgtgtggac agatattgtg aatcgcatga attttgccac tttctgttaa
    12301 aaacaaccct acctctttgc ctttgacaat aacctttttt ccaattaggg gtgttaattc
    12361 atctatagtt gtcactttaa ttttttcttt tgtttccatg tattacacct tctccacttc
    12421 aaaaattcta cgtgcttgcg cattgctagt tattgcttcc caaggttcag cttcgactgc
    12481 ttttttagca tccataatgc gttcaaatag ttcattttgt ctttctgggt caagtaagac
    12541 ttcttttaca ttttcaaatc caagtcttct taaccatggc gctgttcttt cagcatatat
    12601 acctgtttcg cgataatatt gcatcaaagc accacataat ttgattactt catcttctgt
    12661 ttctacagtt gttaaaaatt cagctttttc aacttctgta ccaccattac caccgatata
    12721 gatttggaat ccattttcaa ctgagataat accaaaatct ttaacacctg attcaacaca
    12781 acttcttggg cagcctgata cacccatttt gaatttatga ggtgtatcga tgtattcaaa
    12841 tgttttttct aaacgaatgc caagtcgtgt cgtgtattgc gtaccaaatc gacaaaactc
    12901 tttaccaaca cagcttttaa ctgagcgtgt tttcttacca taagctgatg ctgaacgcat
    12961 acctaggtct tcccatatat ttggtaattc ttctttttta actccataca aaccaacacg
    13021 ttgtgaacct gtcactttaa ctagtggcac atgatatttc ttagccactt ctcctagacg
    13081 aatcagttgg tctgcatctg taacaccccc acgcatttga ggtataacag aaaatgtacc
    13141 atcattttga atattcgcat ggtaacgttc gttagcaaat cttgattctc tttcatcttc
    13201 atgatcatgt ggataaacca tgtttaaata atagttgatt gctggtcgac attttggaca
    13261 tccaccttta tttttaaagt ttaaaacatg tcgaacttct ttagatgttt ttaaaccttt
    13321 cgctcttatt tgcgttacta tttgatcgcg tgtcaaatca gtacaaccac atataccagc
    13381 aggttttgcg gcaacaaagt catctcctaa ggtgtgctgc aatatttgag caatttgcgg
    13441 tttacattta ccacatgaat tccccgcttt tgttttagcc gttacttctt caactgttgt
    13501 aaagccattt tccgtaatcg catttactat agtaccttta tcaacaccat tacaaccaca
    13561 aattgtttca tcatcagcca tatcagcaat tgatagcgat gcctcttctc cacctttagt
    13621 aagcaatgat acaagtgtgt aatcttcagt ggattcacct tttttcatca tgttataaaa
    13681 gcgtgaacca tcatcgatat caccatatag tactgcacca actacattac cgtcttttaa
    13741 aaagattttt ttatagttat tatcaacact attaaatatt tcaatacctt taatttctgc
    13801 attttctaca atttgaccag cactatacaa gtcacaccca gaaactttta atgacgtaaa
    13861 tgttgttgat cccttgtatc cgttcgtttc tttatttgtt aaatgatcag ctaatacttt
    13921 accttgttca tatagtggtg caacgagtcc ataaactttg ccgttatgtt ctgcacattc
    13981 accaactgca tatacattgc tatcacttgt ttgcatcaca tcattgacaa caataccacg
    14041 attaacatct agacctgatt ctttggctac ttctgtgtat ggtcgtatac ctactgccat
    14101 aacaactaag tctgccggaa tctcgcgtcc atcagccaat ttaacaccct caacatcatc
    14161 ttctcctaag atttcagttg tgttggcttg catttcaaac ttcatacctt gcttttctag
    14221 atctgcttta agcatatttc cagctttacg gtctagttgc atttccatca accattcagc
    14281 taaatgtaac accgttactt ccataccttg atctaataaa ccacgtgcac actctaaacc
    14341 tagtaatcct ccaccaatta caattgcttt ctttttagtc ttagcaatgt tcatcatttg
    14401 ttcagtatca tcaagcgttc tccaaccaat tacactcggt aatgtcgaac ctggaactgg
    14461 aatgacgaat gctttagaac ctgtcgcaaa aatcaattta tcgtatgata cttcaatacc
    14521 atttgcagta gtaactgatt gatttgctct atctacttca attacaggat catttgtaat
    14581 taactcgata ccatgttcct cataccactc atatggattc ataattgttt cttcaactgt
    14641 cattttattt tgtaaaatat ttgaaagcat gatgcggtta tagtttggat aaggttcttt
    14701 acctattacc gtaatatcat ataaatcgtt ggcgcgctct aatatttctt cgattgttcg
    14761 aatgcccgcc ataccgttac caatcattac tagtttttgc tttgccataa aatatgcccc
    14821 tttactccat aatatttatt tcaaaaaaag gtattaattt ttcgttagtg cttttatatt
    14881 ttcattggaa tcattaagct ttctaatcta tcgttaatga tttgctttaa aattgggtcg
    14941 aagttaattg aaggtgtgaa gtgtatatct gtattaataa ccatgtcatt catttgctgc
    15001 ttcactttgt taacaagtct tccgtcatat aaaaataatg gtacgacaat caatttttga
    15061 taccgtttcg agatgctttc taaatcatgt gtaaaactaa tctctccata tagcgttctc
    15121 gcatatgtcg gcttgctaat ttgcaaattt tgagcgcata tttgtaactc ttcgtgtgcc
    15181 ttagtaaact ttccattaat attgccgtgt gcaacaacca taactccaac ttgttgttcg
    15241 tcacctgcta atgcgtcaca aatacgttgt tcaattaatc gtctcattaa aggatgtgtg
    15301 ccaagtggct cgcttacttc tacctttatg tctggatacc gtcgtttcat ttcatgaacg
    15361 atattcggta tatccttgag ataatgcatt gcactaaaga ttagcaatgg tacaatttta
    15421 aaatggtcaa ccccactttg aatcaacgtc gtcattaccg tctctaaatc ctgatgctca
    15481 ctttctaaaa acgcaatatc atagtgatgt atatcatctt ttactaattc agaaataaat
    15541 gcttctaacg cttgattctg tcgtccgtgc ctcatgccat gtgcaacaat gatattccca
    15601 ttcacattta ccaacccttt cacacgtatt gtataccaaa tcattttgtt tttgtgaaaa
    15661 gaatcacatt ataatgtaaa atcagggaat tccctgatgc ctgtagtcat gcatattcct
    15721 tatacatttt ccctttttgt taaatcaaaa aaagcgaccg atatatgaat ccctactcaa
    15781 catttatttg agcaagcatt aatatatcgg tcgcttgtag tgtatattat tatcttaaaa
    15841 tggtggttgg cctaatattg tttcgtcaaa gcgctcgggt atcaatactt tgcgcatgat
    15901 cacacctaaa tcgccatcat cattttcatg ttcgctgtat atttcataac ctcttttttc
    15961 ataaatttta agtaaccacg gatgcaatct tgcagatgta cctaaagtaa ctgccgctga
    16021 ctttaacgta tctcgcaaaa atgcttcttc aacataagta agtaattggc taccatagcc
    16081 tttcccttca tactcaggat ttgtcgcaaa ccaccagaca aaaggataac ccgaaatact
    16141 tttcacactt ccccaaggat atctaaccgt aatcgtagat ataatttcat catcaattgt
    16201 catgacaaat gtagtatttt tatctatatt ttctttaaca gcatctaaat tagcattaac
    16261 cgaaggccaa tcaataccta gttctcttag aggcgtaaat gcttcatgca tgagttgttg
    16321 caatttttct gcatcttgtt cacttgcgag tcgaatcatc gtttttgtca tattaatccc
    16381 cactcttttt taaatgattt aaccatattt tatttttaaa ataaatatcc atcaaagtgt
    16441 atcaataaat ttatcacatg tcagaaagta tgcttcatct gaatacacca atactctcat
    16501 gaaacttatt aaaaattact ctctcaacgt aaaaaaccat tcaaattcat gaatggtttg
    16561 gaagaatgat tcattgttac gctatttaat cactacatct taattattgt tgctctaaac
    16621 gattacgctt accatttaag aaagcataaa cgagacctac aaaaataccg ccaccgacaa
    16681 agttacctaa gaaagcaaaa acgatatttt ttaaaacgtg taaccatgaa actgcatcaa
    16741 ggttaaagaa taccatacct gcatatagac ctgcattgaa cacaacgtgc tcatatccca
    16801 tgtatacaaa gaccacgaca ccacaagcta tgaagaatgc ctttgttaag ccgcctttga
    16861 attgcataga gatgaaaata ccaatattaa taaagaagtt acagaaaata ccttttgtaa
    16921 aaatattcaa ccatgttgaa tcaacagtct ttttctgaac taaagctgtt aaagcttgtg
    16981 tcatttcagg tgtcatgaca tgcgcaaatt tcattaagaa aaataacaca aacccaccta
    17041 aaatatttcc taagaaacaa tataataaaa tccaagtcat cttcttaaca gaaacgactt
    17101 tataatacca gcctactgta aagtacatga agttacttgt taataattca gagttagtta
    17161 atacaactaa aatcaaacct aaactaaacg caatggctcc cattaaattg ataagtccat
    17221 ctacgtgagt actcgcaaac tgcgtcttta ttccgaacat aaatacagta acgattgaga
    17281 gtaaaaatcc ggccatcatt gctttaagca tatatcggcc aggtgtttgc tgtaccatta
    17341 cttgcttcat ttgtactgaa gatgatattg aatcaacaat tgcgcctgtt gaatatgtat
    17401 cttcaactgt tttcttattt tctatcacag tacttccccc atttactttg atcaaataaa
    17461 atttactttc taattatatt agtaaatcaa tgtaattgat agattaaatt tcagaaaaat
    17521 ctttttattc tctcgaaaat gtagttttct ttcataaaaa cagcgagata aatcgcgtga
    17581 ttggctaata tctttatgat ttaagatatt acgacgctca ttttatctcg ctttatgtaa
    17641 agtattactt attctttttt aaatttgaaa agtaatgtaa accatatgca aatacactgt
    17701 aaccaattgc agtacgtaaa ataaacctta taactttatt aggtacacat tttgacgtta
    17761 ctgaaataga agatagtagt gttgtgaaaa tatataatat taaaactttg atataacgtt
    17821 ctaccatcga atcatccctc atttttataa tattacaaga ttattataaa ccgaatgaaa
    17881 caaaaaagtt tttacatatt ttcaacaact ttatttcaat aaagcatcaa gttgatctaa
    17941 agtttgattc ataccttgtt caacacccat gtttatgact tgttgagcgg cttcttttgt
    18001 tggaaatacc gatgttgatg tcactgtcgt tttcgttgta ttgctcttgg aaaatgacaa
    18061 agtaattttc atgcttggca tttttgtatc ttttgcacct tgaggtgtcg caaatgagtc
    18121 aatatattca attaaatatg gacgcttaac cgttttatat tctgctaatg tatagctggt
    18181 cattgtaggt gttttaatcg cataaaatgc atcaccgcct gaaacagcat taaagcgaaa
    18241 cactttggta ctagcgtctt ttggatgaaa ccatttttca aataatgctt ttgtcgtata
    18301 ggcatcaaac accttttcaa tcggtgcttc tatcgttctt gaaaaaataa ttttattatc
    18361 ttcaacttta atcgtcatct tcccactccc tatcttttat atttactcat cttaacatgc
    18421 attgagtaac atatgttacc aaatcatttg ttataaacta tagtaatatt aatcattccc
    18481 tctgttgaaa ttgataacta cgaaataaaa aacacactct attcagctat taacagttga
    18541 gtagagtgtg caaacctgtt gaattaatgc gctaacattt cttctttgat ttggtcttta
    18601 tttaattttg aaggataata tgttggccag ttatccattt cttttaataa cgcgtcatta
    18661 tcattaccca taaagatatg gaagtgttct gcctttttag gtgcaatgtt gtgatcgcta
    18721 aattggatga atttcggtaa gtctttatca ttaccatcga ccaatttaaa cataaatctc
    18781 acgccacggt tacctttagg atatttcaat gttttcttac cattgtattc atatttacca
    18841 gtgtgtttct taccatcttt agtaaatgta atttcatttc cttttatatc aatgttagta
    18901 atatcagttt tatatccttt gtcataataa gcttttaaat ctttagcaga tttcttcgga
    18961 tcattttcag ctttatgttc catcacttca tcaagcgtac cgtcttttaa gtaagggtaa
    19021 acagattgcc attcaccagc ataatcgctt aattcacggt ctttaacttg ctcatcttta
    19081 aaatacccat cagaaattgc tttgtcatgt ttactttcag ctttgtcgtc tttcacttta
    19141 acaccactgt ctaaagcttt accgatattt tcaatattag atttcattaa tgattgatac
    19201 gtaatattat cttttttctg ttgttcttta tttaaagact ccatgttgta gaattttaaa
    19261 ggcttcgcat ctgtttcttt acgaattgtt tcagtcactt tattcgcaac attatcttcg
    19321 taaagaatat atttagcatt gctatctcta atttctttaa caattttagt taattctttt
    19381 tgtgatggat cttcagcatt catgttttga ataccttttt gaacaaaacc ataacaatca
    19441 gctaagtatc caattgattc atgtgaaatg aatactgcat taccttgctt atcttttgta
    19501 acttgcttca tatcgttatc aattttctta agatcgtcgt ttaatttttt gtagtttttc
    19561 tcatagtcat ctttatgttt tggatctttt ttcactaatt catctttaat ttctttagcg
    19621 aaagtttggt taattttagg atctaaccat acgtgtggat catatccacc atgatgatga
    19681 tgatgttctt ctttctcatg atcatgtccc tcatgttcat gctcttcacc atgctcgtgt
    19741 tgatcagtta aaagctttgc tttatctaat ttatcctcta aagacagttt tttatcttta
    19801 tctttgatag tagatgcaac tttcttagca accggatcta aattatcccc tgtatacata
    19861 aacaagtctg acttgcttgc acttaatata tctttttgtg ttggttcata gctatgtaaa
    19921 tctgtccctg ctggatagat tgatgatacc ttcacgtgtt ttccaccaat ttgctctgca
    19981 aatgattgca atggataaac tgtcgtttta attgttacct tgccatcttt atcttttcca
    20041 tcatcattcc cacatgcggc taatgataaa gttacggctg gtacaagaag taacatacct
    20101 aatttctttt tcatagtcac cctcctaaat agtaatcttt acgttttaaa attatatact
    20161 catgctactt aaaatacaag cactgttttt ataaataata taaagtcgaa tcatacttta
    20221 ggtatttacc acttttcgtt ttatctaata catgactatt gtacaaatat gatacagaaa
    20281 actttatagt atttacgaac gtcataatat aagaaaaaaa gacaatctct atcatattgc
    20341 taatagaaat tgccttttca tattgtccta aaataactta ccctattttt atagtgtgac
    20401 ttttagattt aataatggta tttacatgat gctattttta tatgattgtc atctactgta
    20461 taaaccaatc tatgttcgtg attgatacgt ctactataat acccagtcag attcgatttt
    20521 aacttttcag gcttacctat accttccaat gcaccatttc tgtcaatact tttaagtaac
    20581 tcattaatct tcttcaccat ttttttatcg ttctgctgaa aatacttata atcttcaaag
    20641 gcttgaggcg aaaacgtaat atttaaccta gccattatac atctatatct ttcgttatag
    20701 ttttcccacg ttctagatct gcaattgatt gagctaagtg ttcagcatta tttgggttct
    20761 gttggaggta aagtgtttcc atcatggagt tataatctga ttctgacatg attactacgt
    20821 ttttatcatc agtagatgtt acagttacca taacactatc atcatttact tttgtcataa
    20881 gtgcctttaa attctgtcga gcgtatgaat aatttttaat aatcatattt atcgcctcct
    20941 tttaacacac ctcaattgta caaatatctg tacaataaaa caagcgacac agaaaaaaac
    21001 tactaatcaa gatttcgcac ggggaagaat caatacctat cctgtactga ttccctacta
    21061 aacaatgttt acatgataat ttccacctat ttagacttca tacattaaaa agacaatctc
    21121 tatcatatcg ctaatagaaa ttgccttttc gtattgtcct aataaattgg tatcacttac
    21181 tgatctcaac aattaagact atttgatttt atcttttaat aacttctcat aactttcata
    21241 atcatatgga tcttcaactt tctcgccatt aataaatgca gttggtgtcg tttttatatg
    21301 attatctttc gctatttttt tatctttctc tgcagctttc caagacttgc tatcttttgt
    21361 cttgtaatct tttataattt tattttccgt ctctttatct aaatgcagtt gtttaatatg
    21421 tttatctaat agttcttttg ttaaccattc tttattttca tcttgctggg cagcaaataa
    21481 ttgcttttga aaatctaaaa atgatttagg tgcatacatc aatactgcat gactcgcacg
    21541 cgaaccaaca attgagtctt tacctaagaa agctaaattg acaaattggt attccacttt
    21601 gtgattatct atataatttt tacgcaactt tggcatgact ttttcatcta attctttaca
    21661 ataaggacat ttatagtcgc catatacgac aactaatggt ttgccgtttt tcgaagatgt
    21721 cgttgctgat tcttttttac cgcaagctgt taaaattaac atgctcacta taaataatgt
    21781 tagtaatttt ttagtcataa taactcctat tcatatctag ttcgtcatat tttcttcatg
    21841 ataatccgca tcatgttgtt gataatatcg tctcgaatca aaatcttgct tatatgatac
    21901 acgtttatca ctaaaatcat atgcataata atgcacttca tcattactca aaggtttata
    21961 agctaaaatg ataattttat ccttatcata gacataaata ttagcatctt tgcgttcaaa
    22021 atgatcaaca cgatcaccgt tgcctttttt ggcaagttgt tcttgttttt gattttgaat
    22081 tttaactgct tcatctattt ctttcacata tttatcatta ccacatcctg atataaagac
    22141 tagtaataat aataccgtcg ccacaaatcg cttcaatgct cccactccta ctatcaactt
    22201 ccaactaact ttcaatataa ttaatactat acaccgtcaa acattaatgc gctacaacta
    22261 ctaccaatgc atttaatata aatatccaaa gaaataacag ttatgtatag tcaaagaaaa
    22321 atatatttca ttttctatgt gtatatgtaa tcagtttttg ataaaataaa aaaatagcat
    22381 tattaataaa ttcaaaaatt atagttcaat tcagagaggg atgggataaa atgaactttg
    22441 taacgttgac ttcggatgag ttcaatgcgt ttacaacaaa gcatttttca cattacacac
    22501 aatcagctat tcattacaat catagagttg atttaaaagg cgatgtgcat cttgtagggg
    22561 ttaaagatga caatggtcaa gtgattgcag gatgcttatt gacagaagca cgcacactta
    22621 aatttttcaa atatttttat acacatcgcg ggccagtgat ggattataca aatcaatcat
    22681 tagtagcatt tttctttaaa gcattaacgt catatttaaa gaaacacaat tgtttatatg
    22741 tccttgtaga tccatattta attgaaaatt tacgcaatgc agacggtgaa attgttaaat
    22801 cttatgataa ccgagcattt gttagaacaa tggataaatt aggttataaa caccaaggtt
    22861 tccctgtagg ttatgattca atgagccaaa tccgttggct gtcagtgtta gatttaaaag
    22921 ataagactga agaccaactt ttaaaagaaa tggattatca aacgagacgt aatattaaaa
    22981 aaacatatga tattggtgtc aaaactaaaa cgttaacgat tgatgaaacg caaacttttt
    23041 tcgacttatt ccatatggct gaggaaaagc acggtttcaa attccgtgag ttaccatact
    23101 ttgaagaaat gcaaaagtta tacgatgacc acgccatgtt aaagttggcg tatattgatt
    23161 taaacgagta tttaaaaacg ttacaattaa agcaacaaca attaacagct gaactctcag
    23221 gtgttgaaga agcattggaa gaaagtccta attcaaagaa aaataaaacg aaacgcacac
    23281 agctagaaca acagctaaat agcaataagc gtaaaatcga caatacaata gaacaaattg
    23341 aacaagatgg tgcagtctta aacttagctt ctgctttatt tatctacaat gagcatgaag
    23401 tttattactt atctagtggt tcaaacccta aatataatgc ttatatgggt gcctaccatt
    23461 tgcagtggga aatgattaag tttgctaaag cgcatcatat tgatcgctat aatttttatg
    23521 gtattaccgg tgacttttca gagtcatcag aagactatgg cgtccaacaa ttcaaaaaag
    23581 gttttaacgc acatgtagaa gagtatatcg gtgactttat taagcctatc aaaccattac
    23641 tatataaggt acaaacgtac ttaaatcata aacgtcgtta aaatatcgct ccaagattat
    23701 gacaacataa caaagaacca cgcatcatgt atatggcttc atcgccatac taaaacatga
    23761 tacgtggttc tatttgatta ttcttcattt ataacattta agaaacgtct taattcttct
    23821 gttttcggac gattgaacat ctcttctgga gccccttgtt ctccgatcat gccttcatga
    23881 ataaatacaa tgttattaga tacttcttta gcaaaacgca tttcatgtgt cacaatgacc
    23941 attgtcatgc cttcattagc caaatcttta ataaccttta aaacatcatt cacaagttca
    24001 ggatcaagtg cagatgttgg ttcatcaaac aacatcactt tagggttcat tgctagtgct
    24061 cttgcaatag caacacgttg ttgttgacca cctgataatg catgtggacg ttgatctttg
    24121 acatgtgtta aaccaacttt ctcaagtaac tcaagtgatt tcccacgtgc ctcatccttt
    24181 ttcaactttt taactgtgat aagaccttcc attacatttt ctaatgccgt cttatgcgga
    24241 aaaaggttat aactttgaaa taccatacca gactgtttac gaacttctat ttgtgatttt
    24301 ttatctttag atgtatatgt tttgccgtta acataaactg taccttcagt tggaatttct
    24361 aatgcattaa tcatacgtaa caatgttgtt ttacctgaac cagatcgacc gattaaggtt
    24421 acaacctcac cttgttcaac agataaatca atacctttga tgacttcaac atcattaaat
    24481 gatttatgga tattgttcaa ttgaatcatg agcgataccc tctttcaata taagattcat
    24541 agataccttg gataattgaa ataataaagc ataccaccca gtacattagc gcaactaaca
    24601 cataaattgt taaatattca tatgttgtag acgcaacttc ttgagctttt ctaaacattt
    24661 cagccactaa aataaatcct aataatgatg tatctttgat taaacttaaa aatgtattac
    24721 ctagtgcagg aatcgacact cgaattgcct gcggtaaaat gatacgttgt atcgtttgac
    24781 catacgtcat accgatggaa tatgcagctt ctgtttgtcc tttcggtata gaaataatac
    24841 cgccacgaat aatttccgaa gcatacgcac ctacatttaa tgataaacca ataatagcag
    24901 ccactacagg tgccaatgtc cattggttgt cagcgtcatt tgttaataat ctacctaatt
    24961 ctggaatacc ataaaatatg ataaatagtt gtactatcat tggtgttcct cgaataatag
    25021 atacatagac acgtgaaata cttcttaaaa ttttactcgt tgaaattcgc attaatgctg
    25081 taaataatgc aataatcaat cctaaaacaa atgtaactaa tgtaatagga attgaatatt
    25141 tgaccaaacc ttctagcata ggtccgaaag cttgttttgc agcatctagc gcatgttgtt
    25201 gttcgctatt tagatttaga aacatcttga ccaaaccatt tcttacctat tttagctaat
    25261 tcaccgtttt cctcgatttt tttcaagcca tcattgaatt tttgaactgt ttcatcatct
    25321 gcttttttag aaaatgcaaa tgcagatcta ctttgttcag cattaccttt gattgctttg
    25381 atcttagcat taggtttttg ttttttataa tccaagtatg acagactatc attaaatgta
    25441 ccatcaacac gcttagacaa taataaatcc attgattggt taaagccatc aacttttgta
    25501 atatcagcac ctttatcctt agctaattta ccataattag atgtgaatgt ttgtgctaac
    25561 ttcttacctt taacatcatc aaaatcttta atatcttttt cattttcacg aataactaaa
    25621 acagcacttg agaatgtgta aggcttagaa aatttatatt tcttttctct atcaggatta
    25681 atacctactt ggttcgcgat tacatcaaaa cgccctgcgt ctaaacctgc aaacatagaa
    25741 tcccaagaag tttcattaaa tttaagtttt aaaccttctt ctttagccac tgctttaata
    25801 acatcaatat cgtaaccagt taatttacca tctttatcgt ggaaactaaa tggtgcataa
    25861 gtcccctcag tcccaacatt aattgtcttg ctatctttac tagcttcctt gtctttcgac
    25921 gaattgtttc cgcatgcagc caatatgaaa acgaaagcta tcatcacaaa taaaagtctt
    25981 ttcatagaat atatctcctt attcttatta ttctaatcgg aataaaaata ttttaatctg
    26041 aaatgccgga taagtcaata tgttgaactt ttgctaaaaa gctttatata gctagcaact
    26101 tatatttatt gacgagaatc aacttctttt ttcttacctt gtaacataaa tgaacaaaag
    26161 aaactaacaa ctgtaatcaa taatgcaaat aaaaatccag cgtgataacc atgcaacatc
    26221 gtatcaactt taatttgatt catcatatca attttgttca tgccattata gtctgacata
    26281 gttggagaaa agtttttgct tgcatgggat aaaatagtaa ttaatgcagc tgttccgata
    26341 gaaccagcaa tttgttgtac tgtgtttgtc atagatgaac catgtgcatt catttctggc
    26401 tttaattgat tcatcgtatg ggtcattact ggaattaatc ctaacgcgat accaaccatt
    26461 cgaatcgcat aaactgttgc caacatgatt gttgatgttt gttcatccat tacaacaaaa
    26521 taagccgtag taataacaac aatcgccata cctactaaag caagatttct aggaccaaac
    26581 ttttcatata aagcacccga agtcatcgac ataaatgcca taacaattgc tcctggtaat
    26641 aaagttaatc cagaatctaa agctgaacga tgcaataaat tttgcacaaa gataggtaaa
    26701 atcgtttccg aaccaatcat cgataccatt gtgacaccca taataatgat accaactgta
    26761 aattgtttat ttttaaatac agcaaaattt aataatggtg ttgatagccg atattgacgt
    26821 aatacaaata atgcgactaa aacaacgcct ccaatcatag atactaatac gataggatta
    26881 tcaaaacctt tttctgaaat tgaactcgtt ccatacaata acccgccgaa acctagtaca
    26941 gaaaaaatga cagaaataaa atcaataggt actttaattg tagttccaac attttttatc
    27001 gttttaaatc caattaaaaa tgtcacagct gcaattggag cgacaacatg gaaaagcgat
    27061 ctccagttaa aatattcaac aaggtaacct gaaagagtag gtccaatagc tggtgctaaa
    27121 ccaattacca aaccaaatat ccccatatat ttaccgcgtt catgtggttg gaaaacatcc
    27181 aaaatcgttg tcatcatcaa tggcatcata atacctgcgc ctaacgcctg aataattcta
    27241 gcaactaata atgttgcaaa gtgcggactc aagccgccga gtaatgtacc taagaaaaag
    27301 atagaaatac ccactaaaaa cacttgtctt gttgtataac gttgtataac aagcgcggac
    27361 aaaggaataa caatcccatt agtcaataaa aaagctgttg tcagccattg aacttgtgta
    27421 tatgaaattt caaaatcttt cataatactt ggtaaagccg tcactaatag tgtttcattt
    27481 aataatccaa aaaatccacc catcagcata gtaataatca tcgttaattt ttgtttcttc
    27541 gacatttaaa gcttccccct caccatctaa aaaacgacac acatttttag atatccggta
    27601 attataccca ttttccaaaa aattagcacg actttattac aatttcttta aaacgtaatc
    27661 attactattt acatatgagt agttatcggt cataatatat ttacaaggag ggatagagat
    27721 gaaattagat ttacagactg ctcgtcgtaa tttaaatagc cctaacatta aaacaagaaa
    27781 gcgtgcttta aagattatta agcaacataa aagagcgaaa taattgtttt aatttgacta
    27841 tattgtatgt ctaactatat tttcgagtat ccctagctcg ttaatataaa ctctatactt
    27901 aactatttcc ttacattact tcacatattt aagagctgca tatacactca ctccttgtgt
    27961 acatgcagct ctttttttat aagtagtatt tatctataac ttctaaatca tccgttaatt
    28021 cataaacaag cggtgcacct gttttaattt cataattaat gatatcttca tctgacacat
    28081 cttcaagata tttaatcaat gcgcgaattg aatttccgtg tgcagaaact aataccgttt
    28141 gaccatctag caaatattgt gaaatatgat ctgtccaaaa tggtatcact cgaactaaag
    28201 tatctttcag actttcagaa taaggcatca tacgtttatc taaatgatta tatcgacgat
    28261 cagctaagta agcttcacgt tgttcttcgg tttcagcagg tggtttcaca tcataagaac
    28321 gacgccaaat atgtacttgt tcttctccaa attcttttct agcatcatct ttatttaagc
    28381 cttgcaatcc accatagtgg cgttcattta aacgccagct tttatataca ggaatccatt
    28441 gttgtttaga ttcagttaaa atataatgcg ttgtatctaa agcacgtgtt aataacgatg
    28501 taaaagctac atcgatggca atgttatttt ctcttacttt ttcacctgct ctagtcgctt
    28561 cattaatacc ttgttcagat aaattaacat cttcccatcc agtaaataag tttttagcat
    28621 tccactcgct ttgtccatga cgacataaaa ttaattttgg cataacttat aacctctttc
    28681 ctttaatctt taggacgtta tttcagggat attttaagat gatttaatct tagtaatttt
    28741 cttttcaata tattttaaaa ttcaatgatt aaatcaatta tcactagaca ttaaatacat
    28801 aaatcctatt ccccattttc attttttaat tcataaatga atcaataacc acctaataac
    28861 aaatcatatt atacaccttt gttcgctatt ttt
//
LOCUS       DAKTIP010000012        27208 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAKTIP010000012 DAKTIP010000000
VERSION     DAKTIP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 27208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.012"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1304
                     /locus_tag="O0305_000406"
     CDS             <1..1304
                     /locus_tag="O0305_000406"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCY0273591.1"
                     /translation="ALREQVAFRDDALRIAHEDLYETKSILENVILSTCNRTEVYAVV
                     DQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLRVTSGLDSIVLGETQI
                     LGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIADNAVSVSYAAVELAKK
                     VFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIENAMKLAAKHQVKYDEL
                     SSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVLIDIAVPRDIEPGISA
                     ITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNEWISMLGVVPVIRALR
                     EKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKDPIKQAKELSSDKKSN
                     EKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            1326..2141
                     /gene="ccsA"
                     /locus_tag="O0305_000407"
     CDS             1326..2141
                     /gene="ccsA"
                     /locus_tag="O0305_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCY0273592.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            2183..3109
                     /gene="hemC"
                     /locus_tag="O0305_000408"
     CDS             2183..3109
                     /gene="hemC"
                     /locus_tag="O0305_000408"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCY0273593.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            3131..3799
                     /locus_tag="O0305_000409"
     CDS             3131..3799
                     /locus_tag="O0305_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCY0273594.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            3802..4776
                     /gene="hemB"
                     /locus_tag="O0305_000410"
     CDS             3802..4776
                     /gene="hemB"
                     /locus_tag="O0305_000410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCY0273595.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            4824..6110
                     /gene="hemL"
                     /locus_tag="O0305_000411"
     CDS             4824..6110
                     /gene="hemL"
                     /locus_tag="O0305_000411"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0273596.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGNDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            6171..6272
                     /locus_tag="O0305_000412"
     CDS             6171..6272
                     /locus_tag="O0305_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273597.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            6306..7379
                     /locus_tag="O0305_000413"
     CDS             6306..7379
                     /locus_tag="O0305_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCY0273598.1"
                     /translation="MAMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKNNWLSIIVVSISILLIAIVMA
                     FVFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYIIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7602..8162)
                     /locus_tag="O0305_000414"
     CDS             complement(7602..8162)
                     /locus_tag="O0305_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCY0273599.1"
                     /translation="MNECAFGTKDPVYLDYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLQDCPSKPKHN"
     misc_binding    8220..8521
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8565..11195
                     /locus_tag="O0305_000415"
     CDS             8565..11195
                     /locus_tag="O0305_000415"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCY0273600.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            11208..12479
                     /locus_tag="O0305_000416"
     CDS             11208..12479
                     /locus_tag="O0305_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCY0273601.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            12749..13456
                     /locus_tag="O0305_000417"
     CDS             12749..13456
                     /locus_tag="O0305_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCY0273602.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            13453..14139
                     /gene="radC"
                     /locus_tag="O0305_000418"
     CDS             13453..14139
                     /gene="radC"
                     /locus_tag="O0305_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCY0273603.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            14207..14491
                     /locus_tag="O0305_000419"
     CDS             14207..14491
                     /locus_tag="O0305_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273604.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(14608..14805)
                     /locus_tag="O0305_000420"
     CDS             complement(14608..14805)
                     /locus_tag="O0305_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273605.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            14965..15438
                     /locus_tag="O0305_000421"
     CDS             14965..15438
                     /locus_tag="O0305_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCY0273606.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKSENAYQ"
     gene            15512..15631
                     /locus_tag="O0305_000422"
     CDS             15512..15631
                     /locus_tag="O0305_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273607.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            15831..16673
                     /gene="mreC"
                     /locus_tag="O0305_000423"
     CDS             15831..16673
                     /gene="mreC"
                     /locus_tag="O0305_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCY0273608.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            16673..17203
                     /gene="mreD"
                     /locus_tag="O0305_000424"
     CDS             16673..17203
                     /gene="mreD"
                     /locus_tag="O0305_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCY0273609.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    17230..17357
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            17370..17678
                     /gene="rplU"
                     /locus_tag="O0305_000425"
     CDS             17370..17678
                     /gene="rplU"
                     /locus_tag="O0305_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCY0273610.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            17684..18004
                     /locus_tag="O0305_000426"
     CDS             17684..18004
                     /locus_tag="O0305_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCY0273611.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            18016..18300
                     /gene="rpmA"
                     /locus_tag="O0305_000427"
     CDS             18016..18300
                     /gene="rpmA"
                     /locus_tag="O0305_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCY0273612.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            18655..19947
                     /gene="obgE"
                     /locus_tag="O0305_000428"
     CDS             18655..19947
                     /gene="obgE"
                     /locus_tag="O0305_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCY0273613.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            19960..20415
                     /locus_tag="O0305_000429"
     CDS             19960..20415
                     /locus_tag="O0305_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0273614.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            20429..21031
                     /gene="ruvA"
                     /locus_tag="O0305_000430"
     CDS             20429..21031
                     /gene="ruvA"
                     /locus_tag="O0305_000430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCY0273615.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            21063..22067
                     /gene="ruvB"
                     /locus_tag="O0305_000431"
     CDS             21063..22067
                     /gene="ruvB"
                     /locus_tag="O0305_000431"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCY0273616.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            22069..23094
                     /gene="queA"
                     /locus_tag="O0305_000432"
     CDS             22069..23094
                     /gene="queA"
                     /locus_tag="O0305_000432"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCY0273617.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLIEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSSRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            23117..24256
                     /gene="tgt"
                     /locus_tag="O0305_000433"
     CDS             23117..24256
                     /gene="tgt"
                     /locus_tag="O0305_000433"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCY0273618.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            24275..24535
                     /gene="yajC"
                     /locus_tag="O0305_000434"
     CDS             24275..24535
                     /gene="yajC"
                     /locus_tag="O0305_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCY0273619.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            24810..27089
                     /gene="secDF"
                     /locus_tag="O0305_000435"
     CDS             24810..27089
                     /gene="secDF"
                     /locus_tag="O0305_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCY0273620.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         9531 a         3726 c         5200 g         8751 t
ORIGIN      
        1 ttgcactaag agagcaagtt gcttttagag atgatgcctt acgaattgcc catgaagatt
       61 tatatgaaac taaatctatt ttagaaaatg tcatattatc aacatgtaat cgaactgaag
      121 tatatgctgt tgttgatcaa attcacacag gtcgttacta tattcaacga tttctagctc
      181 gtgcatttgg atttgaagta gatgatatta aagcaatgtc agaagtaaaa gtgggggacg
      241 aagcagtaga acatttattg cgtgtcactt ctggtttaga ttcaatcgta cttggagaaa
      301 ctcaaatttt aggtcaaata agagatgcat ttttcttagc gcaaagcaca ggtacgacag
      361 gaacaatttt taatcatcta tttaaacagg caattacttt tgcaaaaaga gcacataatg
      421 aaacagatat agctgataat gctgtaagtg tgtcttatgc tgcggtcgag ttggcgaaaa
      481 aagtatttgg caaattgaaa agtaagcaag ctatcattat tggtgcaggg gaaatgagtg
      541 aattatcact attaaatctt cttggttctg gaattactga tattacagta gtaaatagaa
      601 caattgaaaa tgctatgaaa ttagcagcaa agcatcaagt gaaatatgat gaactatcat
      661 cattaccaaa tttacttgaa agtgcagata ttgtgattag ttcaacgagt gcacaatctt
      721 atatcattac aaatgaaatg atagaaagaa ttgcagaaaa tagaaagcaa gattcactag
      781 tattgattga tattgcagtt cctcgagata ttgaacctgg tattagtgcc atcacaaaca
      841 tctttaatta tgatgttgat gacttaaaag gtttagttga tgcaaactta cgtgagcgac
      901 aattagcggc tgcaacaatt tcggaacaaa ttcctgcaga aatacatgca cacaatgagt
      961 ggattagtat gttgggtgtt gttccagtga ttagagcttt acgtgaaaaa gctatggcaa
     1021 ttcaagcaga aacgatggac agtattgatc gtaaattgcc agggttaagc gaaagagaac
     1081 gtaaaattat atctaaacat acaaaaagta tcatcaatca aatgttgaaa gatcctatta
     1141 aacaggccaa agaattaagt agtgataaga aaagtaatga aaaattagag ctatttcaaa
     1201 atatatttga tatcgaagct gaatgtcctc atgagcaagc gaagcaacaa aaagaaagca
     1261 aggtcaagga aatttcagcg cgacgtatct ttagttttga ataagcatat taatatggtg
     1321 ataatatgca agaaaacctg tttattcgat tcaatgaaat tatattatta atatacttaa
     1381 tcagtatcat ttgctatttt tatgattttg tacaaaaaag tcataagatt agaagtttag
     1441 gcatatattt attggggatt gtttgggttt tacaaacaat ctctttatct atttttatta
     1501 tacaaactag acatattcca ttagggtcta tttcagatgt attctatact ttaagttggc
     1561 tgattatttc aatctcttta attcttaacc ttatcaaagt tttgaatttc tctgtgtttt
     1621 tcttgaattt aatagggtta acacttttag ggatgaatac atttcaaccg acacattata
     1681 ctaataaggt tcagaaaatt gctgttgtag acgagttact gttagtccat atcggtctag
     1741 ctgtgttaag ttatgtcttc tttgccttag cttttgtcaa tgcattactg tatatcatgc
     1801 aataccgtaa tttgaaggaa aaacgttttg atcaaaaata ctttagaatt ggtagcgttg
     1861 ctacacttga atcaattgtg ttttactcaa cgttaagtgg gtggataatt ctcatattta
     1921 gtattatttt aggtacacag tggggtgtaa tttcagttgg tgaacgtata ttcattgacc
     1981 cgaaggtaat actctcttca attattacag tattatatgg aagttatatt ttacttagaa
     2041 tcaacaaatg gttgaattca agatatttaa tatactataa cattatttta ttttgcttaa
     2101 atatgattaa tttatttttt gcaacacatt ttgtgaattg agaaaataat ctagagcaat
     2161 ccgttcctag gaggaattga acatgcgtaa attagtcgtt ggctccagaa gaagtaaatt
     2221 agctttaaca caaagccagc aatttattga taaattaaaa gctgtcgagc caaatctaga
     2281 aattgaaatt aaagaaattg tcacgaaagg cgatcgtata gtagataaac aattgtctaa
     2341 agtcggaggc aaaggcttat ttgttaaaga aatacaacat gaactttttg aaaaaaatat
     2401 cgatatggca atacactcgc ttaaagacgt accaagtgta attccggaag gtttaacatt
     2461 aggttgtatc cctgatagag aattaccttt tgatgcgtat atttctaaaa cacatacacc
     2521 actatcccaa ttgccagaag gcagtattat tggtactagt tcattacgtc gtggtgcaca
     2581 aatattatct aagtatccta atttagagat taaatggatt agaggtaata tagatacacg
     2641 attagaaaag ttacaaactg aagattatga tgcgattatt ttagctgcag ctggtttaag
     2701 aagaatgggc tggtcagatg atattgtaac atcttatctt gatagagata cattgttacc
     2761 tgcaatcgga caaggtgctt tagggataga atgtcgtagt gacgatgaag aactattaac
     2821 attattaagc aaagtacata atgatgaggt tgcaaaatgt gtgactgctg aacgaacgtt
     2881 tttagcagaa atggatggta gttgtcaggt gccaattgca ggatatgcta caatctcaga
     2941 tcaaaacgaa atcgaattta caggtttaat tatgacccca gatggtaaag aacgatttga
     3001 atatacaatg aacggaacag atccggttga gttaggcaaa acagtgagta acaaattaaa
     3061 agagcaaggt gcttatgaaa ttataaaacg cttaaatgaa caacattaaa taatggagtg
     3121 acgtttaaat atgaagccag ttgtagttat gacacaaaca aatgacatgc aaagtgattt
     3181 agtatcaatt attcacaaac cctttattga tataaagcca ctaaattttg atattcattt
     3241 gttaaatcaa cgctatgact ggcttatttt ttcatctaaa aatgctgtga aattctttta
     3301 taaatatcta aaaggaatta atgttgataa cattgctgtg ataggaagta agacagcgca
     3361 atattgtgaa tcacttggca ttcgagttga ttttatgcca aacgactttt ctcaagaagg
     3421 atttttaaaa tcatttaatc aaactaacca aaaaatactt ttgccttcga gtgaattggc
     3481 gagaccattg ttattagcag cgttatctaa agataatgaa gttgttaaaa tagatttata
     3541 tacttcagtg cctaacaaac aaaatataca agatgttaaa gaaatgatag aacatcaaca
     3601 aatcgatgca ttaacatttt caagttcgtc ggcagtacgt tattatttta atgaaggatt
     3661 tgtaccaaaa ttcaagtcgt attttgctat tggagaacaa acagcacgga ccattaaatc
     3721 atatcaacaa ccagtaacaa ttgcagaaat tcaaacactc gaatcactaa ttgaaaagat
     3781 tttagaaagt aggggctaaa aatgaaattt gatagacata gaagattgag atcatcagcg
     3841 acaatgagag atatggttag agagaatcat gtaagaaaag aagatttaat atatccaatt
     3901 tttgtagttg aaaaagacga tgtgaaaaaa gaaattaagt cattgccagg tgtataccaa
     3961 atcagtttga atttacttga aagtgaatta aaagaagctt atgacttagg catacgtgcc
     4021 attatgtttt tcggtgttcc aaactcaaaa gatgatatag gtactggtgc atacattcac
     4081 gatggtgtta ttcaacaggc aacacgtatt gctaaaaaaa tgtatgatga cttattaatt
     4141 gttgcagaca cttgtttatg tgaatatact gatcatggtc attgtggcgt gattgatgac
     4201 catacacatg acgttgacaa tgataaatca ttgccactac ttgttaaaac agcaatttct
     4261 caagtggaag ctggtgctga tattattgcg ccaagtaata tgatggatgg ttttgttgct
     4321 gaaattcgtc gtggattaga tgaagccggc tattacaata ttcctataat gagttatggt
     4381 gtcaagtatg catcaagttt ctttggacct tttagagatg cagcagattc agcgccatca
     4441 tttggggata gaaaaacgta tcagatggac cctgctaacc gtttggaagc acttcgtgaa
     4501 ttagaaagtg atcttaaaga agggtgcgac atgatgattg ttaaacctgc tctaagttat
     4561 ttagatatag ttcgagatgt taaaaatcat acgaatgttc cagttgttgc atataatgtg
     4621 agtggagaat atagtatgac taaagcagcg gcacaaaatg gttggataga tgaagaacgt
     4681 gtcgttatgg aacaaatggt ttcaatgaaa cgtgcaggtg ctgatatgat tattacgtat
     4741 tttgcaaagg acatttgtcg ctatttagat aaataaggtt ttatatttat gattttccat
     4801 aaactgtagg aggaatttac tttatgagat atacgaaatc agaagaagca atgaaggttg
     4861 ctgaaacttt aatgcctggt ggtgtaaata gtccagtacg cgcatttaaa tcagtagata
     4921 caccagcaat ttttatggat cacggtaaag gttcaaaaat ttatgatatc gatggtaacg
     4981 agtatatcga ctatgtacta agttggggac cacttatttt aggacataga gaccctcaag
     5041 ttattagtca tttacatgaa gcaattgata aaggtacaag ttttggtgca tcaacattac
     5101 ttgaaaataa attggcgcag ctcgttattg accgagtacc ttcaatagaa aaagtgcgta
     5161 tggtgtcatc tggtacagaa gctacattgg atactttaag attagcacgt ggttatactg
     5221 gcagaaataa aattgtgaaa tttgaaggtt gctatcatgg tcatagtgat tcgttattaa
     5281 tcaaagctgg ttctggggtg gcaacattag gattgccgga ttctcctggt gtgcctgaag
     5341 gtattgctaa aaatacaatt acagttccat acaatgattt agatgcactt aaaatcgctt
     5401 tcgaaaaatt tggaaacgat attgctggtg taatcgtaga acctgttgct ggtaatatgg
     5461 gtgtcgtacc gccgattgaa ggttttttac agggattaag agatattacg actgaatacg
     5521 gcgcattgct aattttcgat gaagtaatga ctggtttcag agtcggttat cattgtgcac
     5581 aaggttactt tggtgtgaca ccagatttaa cttgcttagg aaaagttatc ggtggaggac
     5641 tacctgtagg tgcatttggt ggtaaaaaag aaatcatgga tcatatagca ccattaggaa
     5701 atatttatca agcgggtacg ttatcaggaa atcctcttgc aatgacaagt ggttatgaaa
     5761 cgttaagcca attaacgcca gagacatatg agtattttaa tatgttaggc gatatacttg
     5821 aagacggttt aaaacgtgta tttgctaaac acaatgtacc aataactgta aatagagcag
     5881 gttcaatgat tggttatttc ttaaatgaag gacctgtaac taattttgaa caagcgaata
     5941 aaagtgattt gaaattattt gcagaaatgt atcgagaaat ggcaaaagaa ggtgtgtttt
     6001 taccaccatc tcaatttgaa ggtacattct tatctacggc acacacgaaa gaagatattg
     6061 aaaaaacgat tcaagcattt gatacggctt taagtcgtat tgtaaaataa atatacggac
     6121 aaattgagag cctgaacttt gttcaggctc tttttaaatg tatataaggc atgggcggcg
     6181 acttgatagt gaaagtccat atatgctttg tagtcaaaac tgctagcgga tattgttatc
     6241 ttaacaaacg tgaagctcaa gcagcaattt agtcatttta ttttttattg aaagaagtgg
     6301 aaaacatggc aatgatatat agaaataatt tcattgtgtt cgttttatca ttttttatta
     6361 gtattctact gtattcatcg cacgtattac ttccatttat gtttggtcct attatcgcat
     6421 caatcatttg tgtaaaagtt ttcaaacttg atattaaatg gccattctta cttagtgaat
     6481 taggtattgt actattaggt gtgcaaatcg gatcaacgtt tacgaaaaat gtcgttatgg
     6541 atattaaaaa caattggctt tcgattattg ttgtatctat ttcgatatta ttaattgcaa
     6601 tagtaatggc atttgttttt aaaaaaattg cacgtattaa tacagaaaca gctattttaa
     6661 gtgttatacc aggagcacta acacaaatgc tggtcatggc tgaacaagac aaacgtgcta
     6721 atttgttagt tgttagctta acgcaaacat cacgaattat atttgttgtt gttttagtac
     6781 cgttcatttc atattttttt catgatggta acatgcatgc gaatggaaag ttaacaaaag
     6841 tcttgccttt atcacaagta ttaaacatag ggcaaatagt tattttagcg atagctatct
     6901 ttatagttta tctaattatg tctaaaataa agtttccaac atttcaatta ttagcaccac
     6961 tcattgtatt aattgtttgg aatttttcta caggtttaac atttacacta gatcattggt
     7021 tgttgaacat ggcacaacta atatatatga ttagaattgg agttcaaatt gcgcatttat
     7081 tgtcagattt aaaaggtaga ctagcaatcg caattacaat tcaaaatatt atgttgataa
     7141 ttggtgcgct aatcatggtt tatatcatac atttctttga taataatcct attaatgaat
     7201 tgtttcttgg tgcagcacca ggaggggtta atcagatagt tttagttgca ttagctactg
     7261 gtgccgatgt tgctatgatt acgagttatc atattttccg aatctttttc atcctattta
     7321 ttattgcacc aggaattaat tactttttaa aatatcgttc gaataagcgt gaagattaga
     7381 aactatcatc catctaagtt gaattatgct taggatagaa ttcattatta aggtttaagc
     7441 aaaggttgta catctatttt tagattaaga tatctaatat tagcagagca ttaacttgga
     7501 actttaactt atgatttgaa taaattttca cattaaaaaa cgagacagtc tattagttaa
     7561 aaatagatga tgtctcgttt actttatgat gctatagcaa attaattatg tttaggcttt
     7621 gatggacaat cttgtaaatg tgcatcatat aaaccggctg cttctaaaaa cgaaaatact
     7681 gttactggac ctaaaaattt aaacccgtat tgttttaaat ctttagatag ttgtgttgca
     7741 gtatcatcaa ctgtgatacg atcagaagca tgttcatact gcaaatcttt aggcttacca
     7801 tttacatatg accataaaaa tttactaaaa ctaccatatg cttgttcaat ttttaaatac
     7861 ccttgagctt gattaacaat tgcttctaat tttttacgat gatgaacgat atttggaaaa
     7921 gtcattaagc ggtcgatatc ttgagcggtc atttgtgcta ccttttctgg ttcgaaatca
     7981 taaaatgctt cttcataggc ttctttcttt tttaaaatag ttaaccaaga tagcccagca
     8041 tgttgtgatt ctaatgctaa aagtttaaac aatgccttgc tatcatagag cggttgtccc
     8101 catacatgat catgatagtc taagtagact ggatctttag taccaaatgc gcattcattc
     8161 attttattcc cacctataca ttgacttatt ttgtcaatca ttatactata agttaagagt
     8221 ttaatatagg atttgggaag agtaagttca aattatttta aagagagcat atgatttggt
     8281 gtgagtatgt aaatgatttt tactgaaggt agcccacaga gtatcttagc tgaacgtgat
     8341 taatagtagg ctaagacgtg tttgagcgtt aatcataatc tagagtgctt gaagagatga
     8401 agcatttcaa atttcacgat gatagtattg gtatgattat tccaattatg ttaaaagagt
     8461 gaatttgata ttaatcatcc ttcttgaata taggtggtac cacggaatat ccgtcctatt
     8521 tgtatatagg atggatattt ttattttttt aggaggtata gcaaatggaa atgaaaccaa
     8581 aatatgatcc tcgtgaagtt gaagcgggac gttatgaaga atgggtaaag aatggttatt
     8641 ttaaaccgtc agaagataaa tcaaaagaaa catatacaat tgttatcccg ccaccaaatg
     8701 taactggtaa attacattta ggacatgcat gggatacgac tttacaagat atcattacac
     8761 gtatgaaacg tatgcaagga tacgatacgt tatacttacc aggtatggat catgctggta
     8821 ttgcgacaca ggcaaaggta gaagctaaat taaatgaaca aggaataact agatatgatc
     8881 ttggtcgtga aaagttttta gaacaggcat gggattggaa agaagagtat gcgtcattta
     8941 ttcgtgcgca atgggctaaa ttaggtctag gtttagatta tagtagagaa cgttttactt
     9001 tagatgaagg tttaagtaaa gcagttaaaa aagtttttgt tgatttatac aataaaggaa
     9061 ttatttatcg tggcgaacgt attataaatt gggatcctaa agcacgtaca gctttatctg
     9121 atattgaagt aatacatgaa gatgttcaag gtgcgtttta tcattttaaa tatccttacg
     9181 ctgatggtga aggttttatt gaaattgcaa caacaagacc agaaacgatg ttaggtgata
     9241 cagcgattgt tgttaaccct aatgacgaac gatacaaaga tgtaatcggt aaaactgtta
     9301 tattaccaat cgtaggacgc gaactgccta ttttagcaga tgagtatgtt gatatagact
     9361 tcggttctgg tgctatgaaa gtgacaccag cacatgaccc taatgatttt gaaattggtc
     9421 aaagacatca attagaaaat attatcgtta tggatgaaaa tggtaaaatg aacgacaaag
     9481 cgggtaaata tgaaggtatg gaccgttttg attgtcgtaa acagctagtt aaagatttaa
     9541 aagaacaaga tttagttatc aagattgaag atcatgttca ttctgtaggt cattcagaac
     9601 gatctggcgc tgttgttgaa ccatatttat caacacaatg gtttgtgcgc atggaagact
     9661 tagcgaaacg ttcattagat aaccaaaaaa cagatgatcg tattgatttt tatccgcaac
     9721 gtttcgaaca tacatttaac caatggatgg aaaatattag agattggacg atttcaagac
     9781 aattatggtg gggtcatcaa attccggctt ggtatcataa agaaacaggc gaaatatatg
     9841 ttggagaaga agcgccaact gatattgaaa attggcaaca agatgaagat gtattagata
     9901 cgtggttctc aagtgcttta tggcctttct ctacgttagg ttggcctgat ttagaaagtg
     9961 aagactttaa acgatactac ccaacaaatg ccttagttac aggttacgat attatctttt
    10021 tctgggtagc acgcatgata ttccaaggct tagaatttac agatcgtcgt ccatttaatg
    10081 atgtattatt acacggttta gttcgtgctg aagacgggcg taagatgagt aaatcattag
    10141 gtaatggtgt ggatccaatg gatgttattg acgaatacgg tgctgatagc ttgcgttact
    10201 tcttagcaac aggttcatct ccaggacatg atttaagata ctcaactgaa aaagttgagt
    10261 cagtgtggaa ctttatcaat aaaatctgga atggggcacg tttcagttta atgaatatcg
    10321 gtgaagactt taaagttgaa gatatcgatt taagtggtaa cttatcatta gcagataaat
    10381 ggattctaac acgtttaaat gaaacgattg caacagttac tgatttaagt gacaaatatg
    10441 aattcggcga agttggacgt gcattatata atttcatttg ggatgatttc tgtgattggt
    10501 acattgaaat gagtaaaatt ccaatgaata gtaatgatga agaacaaaaa caagttacac
    10561 gttcagtatt gagttatact ttagacaata ttatgagaat gctacatcca ttcatgccat
    10621 ttgtaacaga gaaaatatgg caaagtttac cacatgaagg tgacacaatt gttaaagctt
    10681 catggccaga agtgcgtgaa tcattgattt ttgaagaaag taaacaaaca atgcaacaac
    10741 ttgttgaaat cattaaatct gtaagacaat cacgtgtaga agtaaatacg ccattgtcta
    10801 aagaaatacc tattttaatt caagctaaag ataaagaaat tgaaacaact ttatcacaaa
    10861 acaaagatta tttaatcaaa ttctgtaatc ctagtacctt aaatattagc actgacgtgg
    10921 aaattcctga gaaagcaatg acatcagttg taattgcagg taaagtggta ttaccattag
    10981 aagggctaat tgatatggat aaggaaatca gccgtttgga aaaagaatta gctaaacttc
    11041 aaagcgaatt agatagagta gataaaaagc tctctaatga aaactttgta agtaaagcac
    11101 ctgaaaaggt tataaatgaa gaaaaacgta aaaaacaaga ttatcaagaa aaatatgatg
    11161 gtgtgaaggc aagaattgaa caattaaaag cataggagtt agtaacaatg aattacctag
    11221 agagcttgta ttggatacac gaaagaacta aatttggcat caaaccaggt gttaaacgta
    11281 tggaatggat gctagcacaa tttaataatc ctcaaaataa cattaagggt attcatgtag
    11341 gtggcacaaa tggtaaaggc tctacagttg cttaccttag aacagcttta gttgaaaatg
    11401 gttatgaagt aggtacattt acgtcgccgt ttattgaaac atttaatgaa cgaattagtc
    11461 taaatggtgt gccaatatca aatgacgcta ttgtagaatt agtatcacgt attaaaccag
    11521 taagtgaaat gatggaacgt gaaacagatt taggtgttgc aactgaattc gaaataatca
    11581 cagcgatgat gtttttatat tttggtgaaa tacatcctgt tgattttgtc attgttgagg
    11641 ctggattggg tataaagaac gattcgacaa atgtctttac accggtttta tcaatcttaa
    11701 ctagtatcgg tctagaccat acagatattt taggtggtac ttatctagat attgctaggg
    11761 ataaaggcgc gattataaag cctaacgttc cagtgatata tgctgttaaa aatgaagatg
    11821 cattaaaata tgttcgtgaa cgcgcaattg aacaacatgc aaagccaatt gaattagata
    11881 gagaaattgt tgttgtatcg caaaatgatg aatttactta ccgttataaa gattatgaat
    11941 tagaaacaat cattttaagc atgttaggtg aacatcagaa acaaaatgct gcattagcca
    12001 taacagctct tattgaatta aatgaacaag gattaattga attagatttc aataagatga
    12061 tagacggtat tgaatcagtt cgttggactg gacgtattga gcaggtgcat gacaaacctt
    12121 taatcatttt ggatggcgca cataattcag agagtataga tgctctaatt gatacaatta
    12181 aacagtacca tgataaagaa aaagtagata ttttgttctc agcaataaac ggaaaaccga
    12241 ttaacgagat ggtcaaacat ttaagtttaa ttgcgcatac gttttatgca actgaatttg
    12301 attttccgaa agcgttacgc aaagaagaaa ttgtaggtag tattgaaaat gatgaaatac
    12361 aattagtaga tgactacgtt gaatttataa aaaattatca aggtgataca ttagtaatta
    12421 ccggtagtct gtatttcata agtgaagtta aatcaaaatt agactttgaa gcattataaa
    12481 actaaaacta aaatagccat gaagaatcta tttaatcaat aggtagagct tcatggttat
    12541 tttttatgct ctaaatcgta tgtgtttata aggttcaatt tcttgccatt attaattttg
    12601 ccgagcagta atgggttgtt aaagtatcca tcaattatgc ttagcaatat tgaaaaattt
    12661 aaatgaaaaa gcggttcaat tcttgaaaaa tagtgtatat ttacattttg gtaataacta
    12721 actataattt acataaggag gttgtcattt ggtagtattg ttatcttata gttgcagttg
    12781 tatttttagt tttctatatc aatttatatc tatagaagaa acgtcatttg attatttaca
    12841 tagaagatcg aaatgtgatt attgtaattc gtcactcaaa tggtatgaat taatgccgat
    12901 tattagtttt ttattattaa aagggcgatg tcgaaactgt cgaaagcgta tttccctaac
    12961 acatttctta ggggaaacct ttgctttaat acctatcgtc tttattaagt atgatttcac
    13021 atacgtaaat gctacgctat ttataactac atatgttttt ctgcttattt ttactatgac
    13081 cgatatcact tctttaatgt tagattgtcg cttaattata atttattgta tcgtttctct
    13141 ctcgttaagt atgatttatc cagtagcttt tatcattatt agtatgacca cgcatatatt
    13201 ctacttttta tttcgggcat atattggtta tggtgacgtt ttactaatat ctgcactttc
    13261 tttgtttttc cctctccaat tcactattta tgtcatttta tttacatttg tcattgctgg
    13321 tttagttgct ttaattacca tgatatttaa gccgattaaa ctattacccc ttgttccatt
    13381 tatatttatt tcctttttta tcaattcact tttttataat gatatccatc aatttttagg
    13441 aggcgtatat ttttgaaaat taaagaaatg gtaacttcag aaatgccaag agaacgtttg
    13501 ttaagccatg gtgcaaaaag tctttcgaat acagaattat tagctatatt aattaacacc
    13561 ggaagaaaag gattctcgag catagacatt agtaatgaac tgcttaaatc tgcttcgaat
    13621 ctgaatgaat tgaaaaaatc ttcaattaac gatttgataa aagttaaagg gattggatta
    13681 caaaaagcaa ttactttaaa agcagcattt gagttagggg aaaggatggg gagaagagct
    13741 gaaaataatc gtataaaaat aacgcaacca agtgatgttg ctgattatat gattccaaca
    13801 atgaaagatt taacacaaga acattttgtc attttattgt taaattcaaa aaatgtagtg
    13861 attaaagaaa cctgtgtttt taaaggtaca ttaaatagtt cgattgtaca tccacgtgaa
    13921 atttttagta ttgcggtgag agaaaatgcc aatgcaatca tcgcagttca taatcatcca
    13981 tccggtgatg taacgccctc acaagaagat atcataacaa caatgaggtt gaaggagtgt
    14041 ggtttgattt tagggataga tttattggat catattataa tcggtgataa tagatttacc
    14101 agtcttgtag aagcgggtta ctttgatgaa aatgattgaa gttgatagaa ttaatgacgt
    14161 atcttgtgta taatacctac gaagtacttt cattggagga aaaatagtga ctctatttat
    14221 tattatcggg gttctcgtgc caatggttta taccatgcag ttaaatatta aaaatgaacc
    14281 tgtaacaaag cgcaatcttt taataacatt agctttatct acgttaggta ttttagtaac
    14341 cgcgttagca ggtgtaatcg ttacgaaaca agcttttcct ttattaagtg tagcaattgg
    14401 ctcaattttt actggaatcg tttggggcct tttactaagt ggtagctacg cgctgatacg
    14461 atttttatct aacgcatttg ggcgtaagta atagtaatct gttcatcaag tagtatccgt
    14521 gcttgaaaac aaactaaaac tcctaatgtg gaactagtta aaaaattcta aactagtaca
    14581 ttaggagttt tgttatgcag aataaattta attgttaaat tgaaagtgcg gtaaaaatcc
    14641 actatttatt tgaaaaaaat cgagcaacca aattaaacct tgatatccta agtaaataca
    14701 taataaaaca agtccgacat aaattaaaaa tcgcaaaata gaaagtccaa ctctaaaaag
    14761 gaggatgact aatagtgcta ttaaaataat tgttagtata ctcaatgcta caaacctcct
    14821 aatacgcttt taaatcataa caaaattata agctatttta aatgaacgtg catcagtcct
    14881 aagatatatc atgattaagg ctgaagactg aggaaaatat acataataaa atatataatt
    14941 agatgaagac attggaggcg aactatgaga ttgatattta gtatgattaa aaacattata
    15001 gcagtaatag caattgtgat aattgtttat atcgccttga aatacgcacc ttttttaaaa
    15061 gaacaagatt ggaatcctgt aaatcacgat aatgatcaaa tgaatcaagt tacgcaaccc
    15121 actaatgatg cccaacatgt ttatgtaagt ggtgagaaat attcattaaa agaaaatgat
    15181 ttaataaaga atgtaccatt aagccaaatt aaaaatgtat ttaaaatgat agataaacaa
    15241 gagtttatgg ctgtgtctgg aatgaatcgc atggcttata atgatcaata tattataggt
    15301 caaagaggag acgaatttat tctttataaa tttggagatg agtcaatgcg tgtttacaat
    15361 actgaatttg aaatgcaaca agacttaaat gaattagggc aaaatttaca actaaaatct
    15421 gaaaatgctt atcaatagga aattattgca taagaaatat attttagtta tcctgtcact
    15481 agaataaatt taaaaggttg gtgtagttca aatggcagaa caatcaaaac agaaacaagc
    15541 taatgaacaa caaaaggccc agaacttatt cgcacgttgg agacaacttc agaattcgaa
    15601 cagtgaatcc tcgaacgata caaacaaata attttaaaat ttaaatcgat tatttaaact
    15661 actagtgact ggaatgtttg ctagtagttt ttttgttttt aaaatgccac aaatttttta
    15721 cggagttgtt cattttcaaa gtaattcgta gttagtgctt tatttcataa tgggagtaat
    15781 ataaaataga aaaggatata caatattttg acataataga ggtgttctgg gtgcttaagt
    15841 tttttaaaaa taacaaatta attgttgttt tatgtgcaat tatcgttttt attgcattaa
    15901 ttgggctgtc catacgttca caatctcaat cacctcctga acaatatata ggtgattctg
    15961 tgtcttttgg acaacgagtt gtgagttatc cagttaattt tgttgctggt acgattgggg
    16021 acttttttaa aaaaggagat tctaaagaat ctaaaaataa gattagccag ttagaatcta
    16081 agaaccaaca attagaagcg gaaaatgaaa aattaaaaaa agagcttgat ttaaaagata
    16141 tttcaaaatt tgatcctatt tctactacgg ttttggcaag aaatccggat cagtggatga
    16201 atacaattgt aattgataag ggatctaaat caggtataac ttcaaatatg gctgtgatga
    16261 catcacaagg ttttgttgga agagttacta aagttaataa attttcttca caagttgatt
    16321 taatctcaac taatacacgt gcgggtaaat tatctgtaaa tatacaacac ggttctaaaa
    16381 atatatttgg tttaattgat cgttatgatg aaaagaactc agaacttgta attagtgaca
    16441 ttaataatag agataatatc tcaaaaggtg ataaagtcgt tacaagtgga ttagctgatc
    16501 aactaccaag taatttatat ataggagaag tgactaaggt tcaaaatgat caatacggct
    16561 tagctaaaga agttagggtt aagactggtg cggacttaac agatttgagt catgtttatg
    16621 ttgcaaaaag agatcctaaa acaattcctg atgatgaaag cagggataaa taatgcgtac
    16681 actgtattat tttttgatag gtattttact attttatata gatactgcaa ttgggcttct
    16741 tattccaatg cacataggta agtttgaact tgtatttgta ccacacctta catttatgta
    16801 tattttaatg atggtagtgt atcgtggctt tggcgtatca ttattgctca gcatattttt
    16861 aggcgtaatg actgatgtat actttggtag tatttatgga gtgtacttat ttggctatat
    16921 attgttttta gcacttatag atcgattctt taaaattttt tacaaagatc attcgatgtt
    16981 attcattatt atattagcca gcaccttatt attagaagtc tatgtggcat taatatacgg
    17041 tatgttagga ttcattcaat ttgatattat tcattttgta gtctttagat tattgccaac
    17101 attaattatg aattttgtac tgttgattat gctttatccg ttgattataa agttccttaa
    17161 aaaaacaaac aatgacattg acatgaaacg tcgtcaatgg taagatgcaa tagttgagta
    17221 gttataagta gctacataca accgctcaaa tataggttca agaacatttt taatgaagaa
    17281 agcgattttc ccacagagtg caaacgcttg actcaataac gaaatgtcac ctatgtatgg
    17341 cgtgacttat tatacaggag gtgcaaagta tgtttgctat tattgaaaca ggtggaaaac
    17401 aaatcaaagt agaagaaggt caagaaatct tcgttgaaaa attagacgta aacgaaggag
    17461 atacttttac atttgataaa gtattatttg taggtggaga ttcagttaaa gttggagcgc
    17521 caacagttga aggtgcaaca gttactgcta ctgttaataa acaaggtcgc ggtaaaaaaa
    17581 tcactgtatt cacatacaaa cgtcgtaaaa attcaaaacg taaaaaaggc catcgtcaac
    17641 catacactaa attaacaatc gataaaatca acgcgtaatt attatgatta ctgttgatat
    17701 tacagttaat gatgaaggca aagtaacaga cgttattatg gatggccatg ctgaccatgg
    17761 tgaatatggt catgatatcg tttgtgctgg agcttcagct gtattgtttg gtagtgttaa
    17821 tgcgattata ggattgacat ctgagagacc agatatcaat tatgacgaca atggtggtca
    17881 ttttcatata agaagcgttg atacaaacaa cgatgaagcg caactaattc ttcaaacaat
    17941 gcttgtgtct ttacaaacta ttgaagaaga atataatgag aatattagat taaattataa
    18001 gtgaggtgca ttccgatgtt aaaattaaac ttacaattct tcgcatctaa aaaaggggta
    18061 agttctacaa aaaacggacg tgactctgaa tcaaaacgct taggtgctaa acgtgctgac
    18121 ggtcaattcg taacaggtgg ttcaatttta tatcgccaac gtggtactaa aatttaccct
    18181 ggtgaaaatg taggtcgtgg tggcgatgat acattattcg ctaaaatcga cggcgttgtt
    18241 aaattcgaac gtaaaggtcg cgacaaaaaa caagtttctg tatatgcagt agctgaataa
    18301 ttttgtctag ttaacaccag aagtgaatct tctggtgttt tttacttttt ataaaatgtt
    18361 tttcattatt attttcatta tgttattaaa aaatgggaat tttagacgtt atactaaatg
    18421 tgcaactgta taagagcccc taatcactaa actataaggg ggacaaagga atacagttgc
    18481 agcgtttaaa gaataaactg taccacaatt ggtgctgaga aatataagta ttttaaagca
    18541 aagatttata aaagtaactg cataagagcc cctaattatt tacaatatat aaggggctct
    18601 aatatgctat aattattggg aaaatgaaaa ttatatgtaa aagaggtgag atatatgttt
    18661 gtcgatcaag tcaaaatatc tcttaaagcc ggtgatggtg gtaatggtat taccgcatac
    18721 agaagagaaa aatatgtacc atttggtgga ccagctggcg gtgacggtgg taaaggtgct
    18781 tcagtcgtat ttgaagtgga tgaaggttta agaacgttat tagattttag atatcaacgt
    18841 cattttaaag caagcaaagg tgaaaatggc caaagtagta atatgcatgg taaaaatgcg
    18901 gaagatttag tattaaaagt tccacctggt acaattatta aaaatgttga aacagacgaa
    18961 gtgttagcag atcttgttga agatggtcaa agagctgtag tagcgaaggg cggtcgaggt
    19021 ggccgaggta attcacgttt tgcaacacct agaaaccctg cacctgactt cagtgaaaaa
    19081 ggtgaaccag gtgaggaatt agatgtatct ttagaattga aattattagc tgatgtagga
    19141 ttagtaggtt tccctagtgt gggtaaatcg actttattat ctatcgtttc aaaagctaag
    19201 cctaaaattg gggcatatca ttttacaacg attaaaccaa atctaggtgt tgtttcaacg
    19261 cctgatcaac gtagttttgt tatggcagat ttaccaggtt taattgaagg tgcatctgat
    19321 ggcgttggat taggacatca atttttaaga catgtagaga gaacaaaagt tattgttcac
    19381 atgattgata tgagcggttc tgaaggtaga gaacctattg aagattataa agtcattaat
    19441 caagaattag ctgcgtacga gcaacgttta gaagatagac ctcaaatcgt agtagctaac
    19501 aagatggatt tacctgaatc acaagataat ttaaacttgt ttaaagaaga aattggcgaa
    19561 gatgtgccag ttattccagt ttcaacaata acgcgtgata atattgatca attattatat
    19621 gcaatagcag ataaattaga agaatataaa gatgttgact tcacagttga agaagaggag
    19681 tcagttggca ttaaccgagt attatataaa catacaccgt cacaagataa atttacaatt
    19741 tcaagagatg atgatggtgc ttatgtggta agtggtaatg ctattgaaag aatgtttaaa
    19801 atgactgact ttaacagtga tccagcagta cgtcgatttg ctcgtcaaat gcgttcgatg
    19861 ggtattgatg atgcgcttag agaacgtggt tgtaaaaatg gtgatatcgt tagaattctt
    19921 ggcggagaat ttgaattcgt tgaataggag cgaaacatga tggacaataa agattataaa
    19981 aagttttatt taattagaga agatgtcttg cctgaatccg tggttaaaac attgaagatt
    20041 aaagatgcct taaaaagtga tccgacattg tccatttatg atgccgttaa acagtttgat
    20101 ctatctagaa gtgcttttta taaatataga gaaacgatat ttccagtaga cgataaaatg
    20161 cttgaccatc gagaatttac attaatttta tatgtaactg atattgttgg tatgttggca
    20221 cgtgtactag atgttatatc aaagttagaa ctatctgtat taacgattca tcaaagtatt
    20281 ccaatggaag aaaaagcaac aataacatta tcactgaatg ctaaatctaa agaaacttca
    20341 gtagaagatg ttattggcgc tttgagaaat ttagattatg tatcaaaagt agaattaatt
    20401 agtatgagta tgtaaggatg tgcctataat gtacgcgtat gtcaaaggta agttaacaca
    20461 tttatatcct acacacgtag ttgttgaaac tgctggtgtt ggttatgaaa ttcaaacacc
    20521 aaattcttat cgttttcaaa agcatctaga tcatgaagtt ttaattcata catctttaat
    20581 tgttcgtgaa gatgcacaat tattgtatgg atttagtagt gaagaagaga aagatatgtt
    20641 cttgagttta attaaagtta ctggtattgg tccgaaatca gctttagcta ttttagcgac
    20701 aagtacgcct aatgaagtaa aacgtgccat tgaaaatgaa aatgatacgt atttaactaa
    20761 attcccagga attggtaaga aaacggcaag acagattgtc ttagatttaa aaggtaaagt
    20821 gaaaattact gaagaagata gcgattcatt attacaagta gacgctactt cgacggtgca
    20881 agatcaattc gtgcaagaag caatgttagc gttagaagca ttaggttatt ctaaacgaga
    20941 gcttgcaaaa gttgagaaaa cgttaaataa aaataaatat gactcagttg atgaagctgt
    21001 taaggcaggt cttcaattag ttgtatctta attttaaata gattaatagg ggaagtgttg
    21061 tcatgaatga gcgtatggtt gatcaatcaa tgcatagtga agaaactgat ttcgaattgt
    21121 cgcttagacc tacgagatta cgacaatata ttggtcaaaa ttcaataaaa agtaatttag
    21181 aagtatttat taaagcggct aaacttcgtc atgaaccatt agatcatgta ttgctttttg
    21241 gcccccctgg attaggtaag acaacattat ctaatatcat tgccaatgaa atggaagtta
    21301 atatacgtac agtatcaggg ccttcattag aaagacctgg tgatttggct gcaattttat
    21361 caggacttca acctggagat gttttgttta ttgatgaaat acacagactg agtagtgttg
    21421 ttgaagaagt gttataccct gcaatggaag atttcttttt agatattatc attggtaaag
    21481 gcgatgaggc tagaagtatc cgtatcgact tacctccatt cactttggta ggtgcaacaa
    21541 cgcgagctgg cagcttaaca ggtccactaa gggatcgatt tggtgtgcac ttaagattag
    21601 aatattataa cgaatcagat ttaaaagaaa tcattattag aacagctgag gttttaggca
    21661 caggtattga tgaagaaagt gccattgaac ttgctaaacg ttctagaggg actccaagag
    21721 tagcaaatcg actattgaag cgggtaagag acttccagca agtgaatgaa gatgaacaaa
    21781 tatacattga aacaacgaag cacgcattag gtttacttca agttgatcaa cacggactag
    21841 attacattga tcataaaatg atgaactgta ttattaagca gtataatggc ggacctgttg
    21901 gtttagatac gattgccgta acaattggtg aagaacgtat tacaattgag gacgtttatg
    21961 agccatttct tattcagaaa ggctttttag aacgtacgcc acgtggcaga aaagcaacac
    22021 cattagctta tgaacatttt gcaaagtcga atgaggagag agaataacgt gaatattgaa
    22081 gaatttgact atgacttacc agaatcatta attgctcaaa cgcctttaaa agatcgtgat
    22141 catagtcgtt tattagtcat ggatagagaa actggtgaaa tgaaacattt acatttcaaa
    22201 gatatcattg agtattttag acctggtgat acattagtgc ttaacgatac gcgagtaatg
    22261 ccagctagac tttttggttt aaaagaagaa actggtgcaa aagttgaaat gttaatgtta
    22321 actcaaattg aaggtaatga ttgggaagtc ttactgaaac cagctaagcg tattaaagtt
    22381 ggtaataaat tgaattttgg taatggcaaa attatagctg aatgcataaa agaaatggat
    22441 caaggtggac gcatcatgcg tttacattat gaaggtattt tacaagaaag attagatgaa
    22501 ttaggggaaa tgccactgcc accatacatc aaagaacgtt tagatgatcc agatcgttat
    22561 caaacagttt acgctaaaga aagtggttca gcggcagcac caacagcagg attacatttt
    22621 actgatgagt tattaattga aattaaaaat aaaggtgtta atatcgcatt tgttacatta
    22681 catgttgggt taggtacgtt tagaccggtg agcgtagacg atgtgaatga ccacgaaatg
    22741 catagtgaat attatcaaat gacacaagaa acagctgatt tattaaatga tactaagtcc
    22801 aaaggacatc gcattatatc agttggtaca acttcaacac gtacacttga aacaattcga
    22861 cgcgatcatg ataaatttgt tgaaacgagt ggctggacta atatatttat ttatccagga
    22921 tttgatttta aagcaattga tggccagatt actaattttc atttaccaaa atcaacatta
    22981 gttatgctag tatcagcgtt tagtagtcgt gaaaatgttc tgaatgctta taaaacggca
    23041 gtaaatttag aatatagatt ctttagtttt ggcgatgcaa tgttaattat ataaaaagaa
    23101 tgtgaggatt ttgaatatgc ctgcagtaac atacgaacac attaaaactt gtaaacaatc
    23161 aggtgcgcgt ttaggtatcg tgcacacacc acacggttca tttgaaacac ctatgtttat
    23221 gccagttggt actaaagcaa ccgttaaaac aatgagtcca gaagagttaa gacaaattga
    23281 agcaaaaatc attttgggca acacatatca tttgtggtta caacccggaa atgatattat
    23341 caaacacgct gggggattac ataaattcat gaattgggat ggtccgattc ttacagattc
    23401 aggcggtttc caagtgttta gtttaagtaa tttacgtaaa attacagaag aaggcgtgga
    23461 atttagacat catactaatg ggtctaaatt atttttgagt cctgagaaat caatgcaaat
    23521 tcaaaatgat ttaggatctg atattatgat ggcatttgat gaatgtccac cgatgcctgc
    23581 tgaatatgat tatgtaaaaa aatctattga acgtacaaca cgttgggcga aaagatgtct
    23641 agatgcacac caaagacctg aagatcaagc attgttcggc attatacaag gtggcgaata
    23701 tgaagattta agagaacaaa gtgcaaagga tttagtagaa ttagattttc ctggttatgc
    23761 aatcggcggt ttgtcagttg gtgaacctaa accggttatg tataaaatgg ttgaacatac
    23821 agagcagttt atgcctaaag ataaaccaag atatttaatg ggtgtaggat ctccagatgc
    23881 gttaatcgaa tgtagtattc gcggcatgga tatgtttgat tgtgtcttac cgacacgtat
    23941 tgccagaaat ggtacttgta tgacatcgca aggtcgttta gttattaaaa atgcaaaatt
    24001 tgcagatgat ttaagaccgt tagatgagaa ttgtgactgt tatacatgtc aaaactattc
    24061 aagagcgtat atacgtcatt taatcaaggc agaggaaact tttggtattc gtcttactac
    24121 tattcataat ttacattttc tgctaaaatt aatggaagat ataagacaag ccattcgaga
    24181 agatcgtctt ttagatttca aagaagaatt cttcgagcaa tatggattaa atgttgagaa
    24241 cccaaaaaac ttttaagcaa gaggagcgta taaaatgcaa ttttcattac taatatatat
    24301 agtcgtaatt tttgcggtta tgtatttctt gatgatcaga ccacaacaaa aacgtgcgaa
    24361 acagcatcgt gagttgatta ataacattca atctggtcaa agaattacaa ctattggtgg
    24421 tattaaaggt actgttaaag cagtagatga aacaactgtt gttattacag ttaatggtca
    24481 tggtactgaa ttaactttcg aaaaacctgc tattaaacaa gttgaccctt cataatgaga
    24541 agtggtatta aaaaggatga aacgaatagc gtttcatcct ttttaagtta taagtagtat
    24601 gtagaatagc ttatttttat tccaaaaaaa gaaattgtga tacataataa ttgttaaatt
    24661 taaattaaat atatctatga ctatttttac tccctgctct agtatgttaa tatgtataag
    24721 gctaataaat tttgtaagaa agatttaact tgcgttataa aatagtattc aagtattcta
    24781 aatacatacg taaataatga ggtgttcatg tgaagaaaag tagtagaata attgcgttct
    24841 tgcttttagt cgtattgttg tttgccggaa tggctgcaac ttataaaagt gttataaaga
    24901 atgtcaattt aggacttgat ttgcaaggtg gatttgaggt gctttatcaa gtcgatcctt
    24961 taaataaagg cgacaaaata gataaaaaag ccttacaatc aacagcgcaa acattagaaa
    25021 atcgtgttaa cgttttaggt gtttcggaac ctaaaattca agttgaagaa cctaatagaa
    25081 ttagagtaca acttgctggt gtaacagacc agaatgaagc acgtaaaatt ttatcttctc
    25141 aagctaattt aacaattcgc gatgcagaag ataaagtaaa attaagcggt tctgatatta
    25201 aacagggatc tgccaaacaa gaatttaaac aggaaacaaa ccagcctaca gttactttta
    25261 aagtaaaaga caaaaacaaa tttaaaaaag taacggaaga aatctctaag aaacgtgata
    25321 atgtcatggt tgtttggtta gatttcaaaa aaggtgacag ctataaaaaa gaagcacaaa
    25381 agaaaaaccc taaatttatt tctgcagcaa gtgttgatca accaatcaat tctgatagcg
    25441 tagaaatctc gggtggattt aaaggacaag aaggcgttaa aaaagcaaaa caaatcgccg
    25501 aattgttaaa tgcaggttct ttaccagttg acttgaaaga aatttattca aactcagtag
    25561 gtgcccaatt tggtcaagat gcattagata aaactgtgtt tgcatcgttt attggtgtag
    25621 cattaattta tttattcatg cttggattct accgattacc tggtttagta gcgattattg
    25681 cattgacaac ttatatctat ctaacgttag tagcatttaa tttcatttcc ggggttctaa
    25741 ctttaccagg attagcggcg ttggtattag gtgtaggtat ggctgtagat gccaatatta
    25801 tcatgtatga gcgtattaag gatgaacttc gaataggtag aacgataaag caagcctttt
    25861 ctaaagcaaa caaaagttca ttcctaacaa tttttgattc taacttaaca acagttatcg
    25921 ccgcagcagt attattcttc ttcggtgaaa gttcagttaa aggtttcgcg acaatgttat
    25981 tattaggtat tctaatgatc tttgttacag ccgtgttctt atcaagattc ttattatcat
    26041 tacttgtttc atcaaatata ttcaaaaatc aattttggtt atttggtgtt aaaaagaata
    26101 aacgacatga tattaatgaa ggtgtagatg ttcatgacct taaaacttca ttcgagaaat
    26161 ggaattttgt taaattagct aagccattaa ttggagtaag tattttaatt gtagttgtcg
    26221 gtttagttat tctttatatc ttcaagttaa acttaggaat tgatttctca agtggtacac
    26281 gcgtagattt ccaatctaaa caagcaatta ctcaacaaaa ggtagagcaa gttgtaaaag
    26341 atagtggact taaagcggat caaattcaga tcaatggtaa agacaataaa gtagcaactg
    26401 ttcaatttaa agacgattta acgcgtgctc aagataataa attgagtgat aatattaaat
    26461 cgaaattcgg tgacacacct caaattaaca ctgtatcacc aattattgga caggaattag
    26521 caaaaaatgc aatgcttgca ttaatctatg catcgatagg tatcatcatc tatgtatcat
    26581 tacgatttga atggcgcatg ggtctttcat ctgtattggc attattacat gatgtattca
    26641 ttatagtagc gattttcagt ttatttagaa ttgaagtaga tttaacattt atcgccgctg
    26701 tattaacaat tgtcggttat tcaattaatg atacaatcgt aacgtttgac cgtgtacgtg
    26761 aaaacttaca aaaggttaaa gtgattacga caacagaaca aattgatgat atcgttaata
    26821 gatcaattag acagacaatg acacgttcaa ttaatacagt attaacagtt attgtagtag
    26881 tagttgctat actattcttc ggtgctccta cgatattcaa ctttacttta gcattattta
    26941 tcggattgat ttctggtgta ttctcttcaa tcttcattgc cgttccgcta tggggaataa
    27001 tgaaaaaacg tcagttgaaa aaatcgccga aacacaaatt agttgtatat aaagaaaaga
    27061 aatcgaacga tgaaaagatt ttagtttaaa atgaattaag cggtatgtga aacaataaag
    27121 agttcacata ccgcttttta ctttatttaa atttaacaaa atatatataa ataagttccc
    27181 gcattcattt ttaaaaaatg gtataatt
//
LOCUS       DAKTIP010000013        26667 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAKTIP010000013 DAKTIP010000000
VERSION     DAKTIP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.013"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            14..808
                     /gene="lpl8"
                     /locus_tag="O0305_000436"
     CDS             14..808
                     /gene="lpl8"
                     /locus_tag="O0305_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl8"
                     /protein_id="HCY0273621.1"
                     /translation="MALYMSVFLLIIFIVGCRNMKDEQKKEEQTNKTDSKEEQIKKSF
                     EKTLDMYPIKNLEELYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKQGGLSNEGMVLYL
                     DRNTRTAKGHYFVKTFYNKGKFPDRKNYKVEMKNNKIILLDKVEDTNLKKRIENFKFF
                     GQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDKAPVLKM
                     HIDGNLKGSSVGYKKLEIDFSKGGKSDLSVIDSLNFQPAKVDEDDE"
     gene            827..1612
                     /gene="lpl9"
                     /locus_tag="O0305_000437"
     CDS             827..1612
                     /gene="lpl9"
                     /locus_tag="O0305_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl9"
                     /protein_id="HCY0273622.1"
                     /translation="MKSIKRIGLCISLLILIIFVTSCDGDNKIIGDSKEEQIKKSFAK
                     TLDIYPIKNLEDLYDKEGYRDGEFKKDDKGTWLIRSEMKIQLKGENLESRGAVLEINR
                     NTRTAKGHYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKPIDDEKVKKEIEEFNF
                     FVQYGNFKELENYKEDEVSYNPEVPIYSAKYQLKNSDYNVEQLRKRYNIPTQKAPKLL
                     LKGSGNLKGSSVGYKNIEFTFIENKEENIYFTDSIYFNPSEDK"
     gene            1795..2607
                     /gene="lpl10"
                     /locus_tag="O0305_000438"
     CDS             1795..2607
                     /gene="lpl10"
                     /locus_tag="O0305_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl10"
                     /protein_id="HCY0273623.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTILTGFSKSNKPGVLDDE
                     GMVLYLNRNAKKATGYYFVNKVYDDISKNHNEKKYRVELKNNKIVLLDNVEDKKLKQK
                     IENFKFFSQYADFKDLKNYQDGNITTNENVPSYEAQYKMNNSDKNVKKLREIYPITTN
                     NSPNLKLYIDGDIKGSSVGYKKIEYKFSKDKGQETTLRDYLNFGPSEGENVE"
     gene            2597..3925
                     /locus_tag="O0305_000439"
     CDS             2597..3925
                     /locus_tag="O0305_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273624.1"
                     /translation="MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVI
                     NQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYA
                     EENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            3922..4245
                     /locus_tag="O0305_000440"
     CDS             3922..4245
                     /locus_tag="O0305_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273625.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            4264..4578
                     /locus_tag="O0305_000441"
     CDS             4264..4578
                     /locus_tag="O0305_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273626.1"
                     /translation="MTLIEPDMTLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(4868..5128)
                     /locus_tag="O0305_000442"
     CDS             complement(4868..5128)
                     /locus_tag="O0305_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273627.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVTGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            5247..6449
                     /locus_tag="O0305_000443"
     CDS             5247..6449
                     /locus_tag="O0305_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0273628.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKMLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(7108..7170)
                     /locus_tag="O0305_000444"
     CDS             complement(7108..7170)
                     /locus_tag="O0305_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCY0273629.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(7234..7302)
                     /locus_tag="O0305_000445"
     CDS             complement(7234..7302)
                     /locus_tag="O0305_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCY0273630.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(7354..7419)
                     /locus_tag="O0305_000446"
     CDS             complement(7354..7419)
                     /locus_tag="O0305_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCY0273631.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(7451..7516)
                     /locus_tag="O0305_000447"
     CDS             complement(7451..7516)
                     /locus_tag="O0305_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCY0273632.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            8037..9521
                     /locus_tag="O0305_000448"
     CDS             8037..9521
                     /locus_tag="O0305_000448"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCY0273633.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGITLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHIVHPTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYVITHNYFYAVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9534..12239
                     /locus_tag="O0305_000449"
     CDS             9534..12239
                     /locus_tag="O0305_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCY0273634.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPASYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHYFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVAKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVSENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAVAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVIQAPDYVVARLLANNEHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            12401..12763
                     /locus_tag="O0305_000450"
     CDS             12401..12763
                     /locus_tag="O0305_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0273635.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIIFKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(12992..13408)
                     /locus_tag="O0305_000451"
     CDS             complement(12992..13408)
                     /locus_tag="O0305_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273636.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            13446..14120
                     /locus_tag="O0305_000452"
     CDS             13446..14120
                     /locus_tag="O0305_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0273637.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            14157..14891
                     /locus_tag="O0305_000453"
     CDS             14157..14891
                     /locus_tag="O0305_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0273638.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            15069..15182
                     /locus_tag="O0305_000454"
     CDS             15069..15182
                     /locus_tag="O0305_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273639.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEILAHVYSVEPENLM"
     gene            15347..16684
                     /locus_tag="O0305_000455"
     CDS             15347..16684
                     /locus_tag="O0305_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCY0273640.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIVIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            16901..17815
                     /locus_tag="O0305_000456"
     CDS             16901..17815
                     /locus_tag="O0305_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCY0273641.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGAEVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENNNEQEN"
     gene            17799..18941
                     /locus_tag="O0305_000457"
     CDS             17799..18941
                     /locus_tag="O0305_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCY0273642.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            19235..20260
                     /locus_tag="O0305_000458"
     CDS             19235..20260
                     /locus_tag="O0305_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0273643.1"
                     /translation="MIEFRQVSKTFNKKKQKIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGK
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFEDTAINQYFKEKN
                     IQFEEVH"
     gene            20264..20923
                     /locus_tag="O0305_000459"
     CDS             20264..20923
                     /locus_tag="O0305_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273644.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            20960..21802
                     /gene="gmpC"
                     /locus_tag="O0305_000460"
     CDS             20960..21802
                     /gene="gmpC"
                     /locus_tag="O0305_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCY0273645.1"
                     /translation="MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            22134..23138
                     /gene="aaa"
                     /locus_tag="O0305_000461"
     CDS             22134..23138
                     /gene="aaa"
                     /locus_tag="O0305_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCY0273646.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(23323..23592)
                     /locus_tag="O0305_000462"
     CDS             complement(23323..23592)
                     /locus_tag="O0305_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273647.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            23741..24136
                     /locus_tag="O0305_000463"
     CDS             23741..24136
                     /locus_tag="O0305_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0273648.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYVGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            24126..24611
                     /locus_tag="O0305_000464"
     CDS             24126..24611
                     /locus_tag="O0305_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273649.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(24780..25562)
                     /locus_tag="O0305_000465"
     CDS             complement(24780..25562)
                     /locus_tag="O0305_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0273650.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(25559..26635)
                     /locus_tag="O0305_000466"
     CDS             complement(25559..26635)
                     /locus_tag="O0305_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCY0273651.1"
                     /translation="MILGLFCIAFIGAILFTFNNENFYNKPIGQIIDVKHVSSTPTKD
                     AQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFRTHEKVLLHISNKPS
                     DAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSIAILIAIYIHIQHSN
                     INLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSIGLTELIIYMADGKG
                     IKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEILQRTPHISMRRWALA
                     GRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISMNWSLEIARALTGGI
                     GIVLTIPITILFMEIFETLRRAKQ"
BASE COUNT         9307 a         3835 c         5203 g         8322 t
ORIGIN      
        1 gtatataaaa agaatggctt tatacatgag tgtatttctt ttaatcattt ttattgttgg
       61 atgtcgaaat atgaaagatg aacagaaaaa agaagaacaa acaaataaaa cagattcaaa
      121 agaagaacaa atcaaaaaga gttttgagaa aacattagat atgtatccaa ttaagaatct
      181 cgaggagtta tacgacaaag aaggataccg agatggcgaa tttaaaaagg gtgataaagg
      241 gatgtggacg atatatacag atttcgccaa aagtaataaa caaggtggat tgagtaatga
      301 aggtatggtc ttatacttag atagaaatac acggactgca aagggacatt attttgttaa
      361 gacattctat aataagggca aattcccaga tagaaaaaat tataaagttg aaatgaaaaa
      421 taataaaatt atcttattag ataaagtaga agatacaaat ctaaaaaaga gaatagaaaa
      481 ctttaaattt tttggacaat atgcaaacct taaagaattg aaaaactaca acaatggtga
      541 tgtctcaatt aatgagaatg ttccaagtta tgacgcaaaa tttaaaatga gcaataaaga
      601 tgaaaatgtt aagcaattaa gaagtcgtta taatattcct actgataaag caccggtatt
      661 aaaaatgcat attgatggta atttgaaagg aagttctgtg ggttataaaa agttggaaat
      721 tgacttttca aaaggtggaa aaagcgattt gtcagtaata gattctttga atttccagcc
      781 ggcgaaggta gatgaagatg atgaatgatg aggatggtgt gtaacaatga agtctataaa
      841 aaggattgga ttgtgcatta gtttgttgat tttaatcatc tttgttacat cttgtgatgg
      901 tgataataag atcattggag attcaaaaga agaacaaatc aaaaagagct ttgcgaaaac
      961 gttagatatc taccctatta agaatctcga ggatttatac gacaaagaag gatatcgaga
     1021 tggcgaattt aaaaaagatg ataaaggtac ttggctaatt agatctgaaa tgaaaatcca
     1081 attaaaagga gaaaatctgg aatctagagg agcagtttta gaaattaaca gaaatactag
     1141 aacggctaaa gggcattata ttgttagaga agttgttgaa gatagcgatg gaatgacaca
     1201 caatcataca aaaagatatc ctgtgaaaat ggaaaataat aaaatgattc cattaaaacc
     1261 aatcgatgac gaaaaagtaa aaaaagaaat cgaagaattt aacttctttg tacaatacgg
     1321 gaatttcaaa gaattggaaa actataaaga ggacgaagtg tcatataacc cagaagtacc
     1381 aatttactct gcaaaatatc aattgaaaaa cagtgattac aatgttgaac aattaaggaa
     1441 gcgatataat atcccgacgc aaaaagcgcc caaattatta ttgaaaggct caggtaattt
     1501 aaaaggttca tcagtcggat ataaaaatat tgaatttacc tttattgaaa acaaggaaga
     1561 aaatatttac ttcacagata gtatctactt taatccaagc gaggataaat aaatataatt
     1621 actaatataa aaaataacgt tagaacattt aataataaag ttaaaatgaa ttacatgtat
     1681 taaattgaat aagttagttt tgtttgataa cataaaagtt gaataatcac tattttaata
     1741 agttgtatga aatattcaaa tgtttataaa attaaaacag agagatgtga aatgatggag
     1801 tatataaaaa aaattgcttt gtacatgagt gtattacttt taatcatttt tattggggga
     1861 tgtggaaata tgaaagatga acagaaaaaa gaggaacaaa cgaataaaac agattcaaaa
     1921 gaagaacaaa tcaaaaagag ttttgcgaaa acgttagata tgtatccaat taagaatctc
     1981 gaggatttat acgacaaaga aggatatcga gatggtgagt ttaaaaaagg cgataaaggt
     2041 acgtggacta tacttacagg tttttcaaaa agtaacaaac caggagtatt agatgatgaa
     2101 ggcatggtgt tatatcttaa tagaaatgcc aaaaaggcaa caggttatta ttttgtaaat
     2161 aaagtttatg atgatattag caaaaatcat aatgagaaaa aatatcgtgt tgaactaaaa
     2221 aataataaga ttgttctttt ggataatgta gaagacaaaa aacttaaaca aaaaattgaa
     2281 aattttaaat tttttagtca gtatgctgat tttaaagatt taaaaaatta tcaagatgga
     2341 aatataacaa ctaatgaaaa cgtaccgagc tatgaagcac aatataaaat gaacaatagt
     2401 gataaaaatg taaaaaaact tagagaaatt tatccaatta caactaataa ctctccaaat
     2461 ctaaaattat atatagatgg tgatataaaa ggaagctcag taggatataa aaaaatagaa
     2521 tataaatttt caaaagataa aggtcaagag acaacattaa gagattattt gaattttgga
     2581 ccgtctgaag gtgagaatgt tgagtaggaa gtataaaata gatttaaaag caattaatca
     2641 aaatactgag tctacaagtg ctatttcgaa agcgtcatac gaagttgaga atgctaataa
     2701 taatggtcta tcaaaaagag acgtgattaa tcaatttaat gatttgaaaa aaatgaaaaa
     2761 attcccttct aatcttgaat atgttgatag ttacactgat tctcttactg gagtaacaac
     2821 ttctgctttt ttaaataaag atacaggcaa agtaactctc gggatgactg ggactaattt
     2881 acaagacgaa gcctttaaaa agttaaaaga aggtgaattt tcaagacaaa atgttaccaa
     2941 tgctttggaa acagttaaag atggatatgc agatcttaaa atattatatt ctcctgcatc
     3001 tgatcaaaac tatagatatg cgaatacaca agaatttata aataaaataa aaagtaagta
     3061 tgacattgat tttattactg gacattcact aggtggaaga gatgcggtag ttctaggaat
     3121 gagtaatggt attccgaaca ttgtggttta taatccagct cctatttcta taactagttt
     3181 gaatcctaat tccccagatg gaaaacgttt attagaatta tataaaaatt ataaaggtaa
     3241 tattactagg tttgttgcag aaaatgatgc attgacagaa aatctgaaga aatataagca
     3301 ttatgttttt ttcggtaatg ataaagtctt taaaaatggt aaaggtcatg aaatggaagg
     3361 ctttctgacc gaagaagaac aaaaagctat aaaaaaagaa cttaaaaaac tacaaggtta
     3421 tgcagaagaa aataataagt catttgtaaa gaattcaaat aatgctatct ctaaattagc
     3481 tagtatagaa ttacttagag caaatatgat gactacaaat ggaggaggat tatcttcttc
     3541 gcagcaaaaa gttttagaaa gtttaacagc tttaacaatt gcgcagtcat tcagtcaact
     3601 gatagacgat gaaattaatc aaatcaaaaa aatgtataat gaaaagaaaa agaaatttgg
     3661 aaaaaattgg gaagacgctc aaaaagctgg aaaagctgta ggtgaagatt tgagtgtaaa
     3721 tggagttctc aatgctttag atgaaggtca agtgaatgaa agtagtatgg taagagaacc
     3781 tgaacaaatg atatctgcaa aagaaagaca actttcaacg atagggtctt ctgtatcaaa
     3841 ttatattatg agagttagac tcagtattaa tgaaatcgtt gataaagatc aagtgcttgc
     3901 atcacaaata ggtggattac tatgatgttt aatcaaatta ataataaaaa tgaattagaa
     3961 gaatcatatg aatctgagaa aaaacgtata gagaatgaac tgcaaaattt aaatgaactt
     4021 aggcatagaa ctcgaaaaga aaatgagcgt agttatgatg tttttcaata tttgaagcac
     4081 gaaatgaatt atagtgaaga tgcccaaagg aaaatgacga gaaatataga agcgtatgag
     4141 caagaaatca atgagataat tagaaagcaa gaatggaaat tagaagaata taaagaagac
     4201 ttaaaaaagt cttatgaaaa gcagttagat aagctaagtg actgataatt tggaggagat
     4261 taaatgactc taatagaacc agatatgacc ttaagaatgc cagatataag tactacagta
     4321 gaaacactta atctcatatc taaaatggaa gcgcaaaaag aaaatattcg cacagttatt
     4381 gcacctgaac ataagcataa atacaaagat attgaaaacg gattaaaagg tgaagaaaaa
     4441 gtattaattg aacaaatggc gcaacattgc gaagctttta aagctaattt taaaggtgca
     4501 gctcaaggag attgggttaa aagtgccatg tctgagatag acagcattaa ggatgacctg
     4561 aaaaaaatta atagctaaac tacatatcat actactaaat aaagcgacca atgttcagta
     4621 tattcacaac tgacactagg ccgcttcttt ttaatttata ttattcccat gtaaagtaat
     4681 tcgaaccgaa tcgaaatatc acactctcaa ttgtaaaaaa acaacttaaa ttactgtccc
     4741 gcattaaaca tgaccacatt cgtaacagca acaataaaaa tcctcaagta ctaaaagcta
     4801 tgatcacttt atcaaaatca acgcaagtta acctctcccg aactacccgt tactcatcac
     4861 cttcacctta caaaccataa agcgctgcag ttaagtattg aatggcatag ccaattgaaa
     4921 tgaaaatagc aatcgttata atagcactgc caatggtata taaagtatgt ttcgtagata
     4981 atccttcagt ctttgaacca aaaacactga gtagaataaa tgcaatacca gtaacatata
     5041 aaactaaaat gacaaataac atgtttatac acctcgattc tctttcgtga gatatatctt
     5101 tgtctccatt ataaagtaaa gcgctcaata atttaattaa gtatactatt tttttattat
     5161 cgaagattgc gaaagaggaa acgctcctat ataatgtaaa acggaattat tcctatttac
     5221 aaataaattg aagtgagggt atatatatgg ctaaaattcc agttacggta ttaagtggtt
     5281 atttaggctc ggggaagaca acgttgttaa atcatatttt acaaaatcga gaaggtcgac
     5341 gtatcgcggt aattgtaaat gatatgagtg aagtaaatat cgataaagat cttgtcgcag
     5401 atggtggggg actatcgcgt acagatgaaa aattagtcga actttctaat ggttgtatct
     5461 gttgtacact tagagacgat ttattaaaag aagttgagcg tttagtgaaa aaaggtggca
     5521 tcgatcaaat tgttattgag tcaacaggga tttcagagcc agtacctgtt gcacaaactt
     5581 tctcatatat tgatgatgaa cttggcattg atcttacagc gatttgccgt ttagatacaa
     5641 tggttacagt tgtggatgct aaccgcttcg tacatgacat caactcagaa gatttattga
     5701 tggatcgtga tcaaagcgtt gacgaaacag atgagcgttc gattgctgat ttattaattg
     5761 accaagttga attttgtgat gtattgatta ttaataaaat tgatttaatt agtgaagaag
     5821 aactagcgaa gttagaaaaa atgttaagcg cattgcaacc gactgctaaa attattaaga
     5881 caacaaattc tgaagtagat ttaaaagaag tcttgaatac gcagcgtttt gattttgaaa
     5941 aagcgagcga gtcagcagga tggatcaaag aacttgagtc tggtgggcat gcatcgcata
     6001 cacctgaaac agaagaatat ggtatatcat cgtttgtata taaacgtcgt ctacctttcc
     6061 atgctaaaag gttcaatgat tggttagaaa gcatgccaaa taatgtcgtt cgatcaaaag
     6121 gtatcgtatg gctagcacaa tacaatcacg tagcatgttt attatctcaa gcagggtcat
     6181 cttgcaatat tcatccagtt acatattggg tggctagtat gtctgaagcg caacaaacac
     6241 aaatattagc agaacgtcaa gatgtcgcag ctgaatggga tccagaatat ggcgatcgtc
     6301 atacacaatt tgtcattatt ggtacagaat tagatgaaga aaaattaaca aaagaactcg
     6361 acgcatgctt agtcaatgcg caagaaattg atgcagattg gcaacaattt gaagatccat
     6421 atcaatggca aattagacca gcacgataag ttgaatgaag tataatcatt tttgaattgt
     6481 ggcttaattg tttgcaattt atgacaatta ataaaaagtt aaggtgttct taggatcttt
     6541 ttgttttcaa tctatctaaa gtgtgatagt tttgataaag cagaaatttg cattttatcc
     6601 atagggctag gacatgtatg tgtcttagtc ctttttatat ttacgttgat gaaaaatggc
     6661 aaaaccatcg taccaactgt taattgaagg tacaatcatt gtttaacagt cataaaaatg
     6721 agtgaagtaa atgcagctat caatgtacta ttaacactta cggtcacacg agtgtttcag
     6781 tctaaacgtg caacgcaatt ccgcacgtgg ccaatatatc ttgcgctaga tcatgaaaat
     6841 cattaagatt tcgcatgcca aatcatgaat gtctcagaac tcgagtatca aacataaaac
     6901 acgccacaaa aatataacgc gtcagactac aaagatcacg aatattccgt atgtcccact
     6961 aagaacgtcc tatacaaaaa acaccaagac catttatttg caacaaacta acaagactca
     7021 cctcacatca ataaatcaac acaaagcaaa gccaccatcc ctattggtat agtggcctga
     7081 gaattttaag tattcaattc gcttaaatta ttttgcgaaa atgtcgataa ttgctttgat
     7141 gattttaatg atagtaccta caatagccat cgttttgtcc tcctgtatgt tgatttaaca
     7201 tagtgatgag ttgttgatcg ttaatgtttg agattagttg ttacctaaaa atttaccaag
     7261 taaatcttta aagaatttga ataattttgc tacgaattcc atgtgaatgg cccccttcaa
     7321 ataagatgtt catatctatg agatttttat aacttactta ccagtgaatt tctcaattaa
     7381 tcctttaatg aatttaatga ttcctgcaat gatacccatg tgaaagacct cctttgtttg
     7441 ttatgaaatc ttatttacca gtgaattgtt cgattaagct tttgataact ttaatgatgc
     7501 cagcgatgat acccattaag attacctcct ttgcttatga gttaacttca ttgtacatag
     7561 ttatcttgtg cgtaattgat ttttttccta acttgaattg ttatgatcat aactgcgaat
     7621 tttttatttt aaaaggacgg gaaatactgc ctaactgtac aggtgcattt acaaaatctt
     7681 tacaatcatt ggtttaatag aggttaagct catgaatttg acatgaagca gaaatttaat
     7741 tatattttat gttataagtt taaaacgaat aacacgttag gtctcgtcta ggcaaagcat
     7801 atttatgaag atgaattgaa cagacagaag taacatttta tatacatatg tgcatgggag
     7861 cacgcttaaa gagaacactg caacaaggta tatcaacgta ccgtgatgca gtgtctagtt
     7921 aaactgaaac ctgcaatagt aaattttctg ccattatgta cagaatctac tattgtaggt
     7981 ttttattttt atacataagc aattatgcga gagattagaa ataaggaggt tatgcagtgt
     8041 taagttttca attgctattt tcactgtttg ttattgcgct tatcattgca ttgataagtg
     8101 gcttgttgtt tttagcacca gttatgccaa tgagatatat taaattacat ttatacatac
     8161 tagtcatgcc agtattattt gcagtcattg gctttttcgg tattcatggt caacatgtct
     8221 taggtccatt taaaatagat cgtttatctt ggttattagc tggctttgta atggcgcttg
     8281 gctttattat tcaaaagttt tcaatgcgat atttactagg tgatcatcat tatagacatt
     8341 actttccatt gttcactgcg attacgtcgt ttgcatcttt agcatggatg tctgaagact
     8401 taagactgat ggcactctgc tggggtatta cattattatg tttaacattg ctgatgaacg
     8461 ttaatcgttt ttggaaagtg ccacgtgagt ctgcgaaatt atcaagcatg acatttttat
     8521 gtggttggct tgcattcgtt ggagcaattg taactattta tattgcgact ggcgagtggc
     8581 gggtaccaca acatatagtt catccgacat ggtcattgtt gacgaatgta ctacttgtat
     8641 tagctgtcat gataccggca gcacaatttc cttttcatcg atggttgatt gaatctgtaa
     8701 cggcaccaac gccagtatcg gcaattatgc atgcaggaat tgtgaatgca ggtggtgtta
     8761 ttctaactcg ttttgcgccg atatttgata atggatttgc gttatcatta ttacttatcc
     8821 tttctagtat ttctgtattg ttaggatcgg gtattagctt agttcaagtt gactataaac
     8881 gccaattagt gggctctacg atgagtcaaa tgggctttat gttagttcaa tgtgcattgg
     8941 gtgtatattc agcagcgatt attcatttaa tattgcacgg tatttttaaa gcaacattat
     9001 ttttacaatc aggttctatc gtgaagcgat tcaatattcc aaaacaagca tctgctaaag
     9061 acgcttatgg ctggattgtc atgggacgtg tattagctat tatcgtggca tttctatttt
     9121 ggatgagtag tgacagaagt gcatatgaag tgttaagtgc actcattcta ggatggtcat
     9181 tacttgtatc ttggaatcaa atggtagcct ttagtaaagg acgcatggca cgtcttgttg
     9241 gtatgatttt gattgcaatc gtgacattta tctacgtcat cacacataat tatttttacg
     9301 ctgtattaca aaatataaca acacatgcga caacgccgcc tacagtgagt gtcatcatta
     9361 gtgttgttat tttaatcttt ggcagtttat taagtatttg ggtggcgcgg catcgatact
     9421 ctaaggcttt tgcggtattg tacgtgtggt tagttaatct aggtgaagca cgctcgaaag
     9481 cgatagaaag tcatccgaat tatttgaaga agtatttata ggaggtgaaa ggtatgacaa
     9541 cacagttaaa tatcaattca gtcattgaaa atgcgaaacg tgttattaca ccattatcac
     9601 caatttcgat ttttgcagca cgaaatccat gggaaggatt agaagcggat acgtttgaag
     9661 atgtcgcaaa atggttacgt gatgttcgag atgtggatat tttcccaaat aaagcattaa
     9721 tagaaagcgc tgtggcacgt ggtgaattag atgaaagtgt ctttaatcaa cttgttactg
     9781 atatgttact tgaacatcac tacaatatcc cgcaacacta catcaatctt tatattgata
     9841 acattaaaac attaaaagac gtacctgcat catatatgaa tcattcaaat gttgatgttg
     9901 ttgctgatct actattagaa aaatcaaaac gtgatatggc tgaatcatat catcactatg
     9961 atgtacgtcc gatgagtgat gcaataatag atgaacaagg tgagccactt agcgaacaag
    10021 tgaatcgtca aatgattaaa tggacgaaac tttatatcga tcaatttcta tcgagttgga
    10081 caatgccgaa gcgtgagcaa agtttttacc atgcatggtt gcatttagcg caacatgacc
    10141 atagttttac taaagcacag cgccaagtga ttaaaggctt acccaatgat cctgaaatga
    10201 cgatagagtc agtattaact catttttcaa tagatcagga agactaccaa gcttatgttg
    10261 aaggacatct tttggcgtta ccgggttggg caggtatgtt gtattaccgt tcacaacagc
    10321 attactttga acaacatttg ttaacggatt atttggcaat tcggttagtt gtcgaacaat
    10381 tgctagttgg tgatgagttt aagtcagtcg ctaaagattg tgaaagtaga tcggaaaatt
    10441 ggtttaagca aactgttgca tcatggtgtt actacagtga tatgcctagc gatgtattac
    10501 tacaacatga cgtcaatgaa attcaaacgt ttattcattt tgcagcaact atgaataaaa
    10561 atgtatttaa aaatttatgg ctaattgcct gggaaatgac atacgaatct cagttaaaac
    10621 aaaaaattaa agcaggtcat gaaagtgtgg cgggcgcatt agatgtaaac caagtaaatg
    10681 tctcagaaaa tgataacgct aatcagccac attcagtatt gttaaatgac acacaagcag
    10741 ttgatgaaaa taatagcgag ctaaatcaga tgggcacatc aacgaaagcg caaattgcat
    10801 tttgtataga tgttcgttca gaaccatttc gtagacatat cgaagcagca gggccctttg
    10861 aaacgattgg tattgcaggc ttctttggat tacctattca aaaagatgcc gtagacgaac
    10921 aattcaaaca tgattcatta cctgtcatgg taccgccggc atatcgcatt aaagaatttg
    10981 cagaccgcta cgatatgaat gtttatcgac aacagcaaca gacaatgtca tcgatgtttt
    11041 acacatttaa attgatgaaa aataatgtca tgcctagtct gttattgcct gaattaagtg
    11101 ggccattttt aagcttaagt accattgtca attcgattat gcctagaaaa agtcgcgcgt
    11161 ctttacaaaa aataaaacaa aagtggttga aaaagcctga aacaaagttg acgattgatc
    11221 gtgagtttga ccgaacatca gacttacctg ttggatttac tgagcaagag caaattgatt
    11281 tcgcgttaca agcgttgaaa ttgatggatt taaccgaagc atttgcgccg ttcgttgtgt
    11341 tagcaggtca tgctagtcat tctcacaata atccacatca tgcatcactt gaatgtgggg
    11401 cttgtggtgg cgcatcaagc ggttttaatg ctaagttatt agcgatgata tgtaatcgtc
    11461 caaatgtcag acaaggatta aaacaatcag gtgtgtatat tccagagaca actgtttttg
    11521 cggtagcaga acatcatacg tctactgata cgttggcatg ggtatatgtg ccagacacat
    11581 tatcttctat tgctttagat gcatatgaat cattgaatga cgcgatgccg atgatttctg
    11641 aacacgcgaa tcgcgaacgt ttggacaaac tgccaacgat tggtcgtgtg aatcatccag
    11701 tggaagaagc gcagcggttt gcgagtgatt ggagtgaggt acgtccagaa tggggattgg
    11761 ctaaaaatgc atcatttata attggacgac gccaattaac aaaaggcatt gatttagaag
    11821 ggcggacatt tttacacaat tatgattggc gtaaagataa agatggcaca ttattaaata
    11881 ccatcatttc tggtccggca cttgtggcac aatggattaa tttacaatat tatgcgtcga
    11941 cagttgcgcc gcatttttac ggaagtggga ataaagcgac acaaaccgtc acgtcaggtg
    12001 ttggtgtcat gcaaggtaat gcgagtgatc tgatgtatgg cttatcatgg caatctgtta
    12061 tggctgctga tcggacgatg tatcattcgc caattcgttt acttgtcgtt attcaggcac
    12121 ccgactatgt tgtagcaaga ctactcgcga ataatgagca tttcgctagg aaggtgtcta
    12181 atcattggct gcgtttaatg agcgttaatg aggaagggcg ttttaaaagt tggatttaac
    12241 gatgatatgt tgcgatgcta agtaactaat atatcatcat atctacaatg ttctagcttc
    12301 ttaaaatata ctaaaaatga taaacttaat gtaatagttg atgtcgtgat aacgatacaa
    12361 agatgaagta atcaatttta atattattgg agttagtgat atgaaaagaa cgaaaggtga
    12421 aatcgaagct gaaatcagta aagccattac gcaatgggaa aaagatttcc ttggcagagg
    12481 ttccttgtca gttaaatcag acattttaag agatatggtg attattagtt tacaaggtat
    12541 cttaacgcca gctgaatatc gtgtatgtag tacgaatgaa ggattattaa atattaaacg
    12601 aacacgttct gaattagttg aatccggtga gcaagatttg aatgatatca tttttaaaat
    12661 tacaggtatc aaagtgatga gcttccatag tgatttaagt acagttacag gtgaacgtat
    12721 tatcgtattc aaacttgagg ataatttgga aaagcatatt taagaaggtt aggtatgctt
    12781 ctgctggctt attaaagcta tagaagtatg cttggttaaa cagcgcaacg ccatatattt
    12841 gggtgtgctg tttttctatt acatgatgtg tttattacgt ggatgaatgc gtgggtctga
    12901 atatagcccg tgaaaggcgt atgtcataaa atttgcgaat ggtgacaagt cgacgcaagc
    12961 atatttgttg cgagacatgc ccactatcct tttacataaa gagtatatgt gtgacgtagg
    13021 catataatcg ataaagtatt cctaaaaaat taggtataat gaccattgtt gcaataagtt
    13081 tttcggcctt gaaaccgata acgcatagta taataggaat aaaatataat acaaatatcc
    13141 agtaaatcaa atttgaaaat gatggatgaa taggggtaaa gaatctaact aatgtaatga
    13201 ttaacgctat atagacaaaa ataactctat tgatacgcct taccccctct gtataataaa
    13261 tataaatcgt actaaattga aaatagcaaa atattgttat ttcaattata caatgtttta
    13321 tttgcaatat acataacaat atgaacttta taattttgat attgtcggta aagtgaaatt
    13381 tgtggcacat tgatattatg gataacattt gtaaaatgaa aatgatgata cgaggagatt
    13441 gtaagatgat agataaaaaa ttaacatcac cgaaaatgac agtgccttta tttttaatcg
    13501 cgctgattgt atttataggt atgttttaca gtgtagtgac aaatcaagaa tggcttaaaa
    13561 atatagatat gggatcatta acatggttta cagattattt cggtgagcca caacgtcagt
    13621 atgttaacaa tttgtttaat tactatatga cgtttagtgc ggaaattgga gatgtcaaag
    13681 gtgtcgtgtt gatttccatt atcgtcacaa tcatactgtt tattaaacag aggcatttag
    13741 cggtttggtt tgtgacatat ttggtttcag gtgtcatcat gaacaaatta attaaagata
    13801 ctgtattacg tccaagacca tataatcatt tagccgttga tacaggcttt tcatttccga
    13861 gtggacattc caacgccagc acattattat atttcgcctt aatgatcatt attatttcac
    13921 ttgctgctaa gacaataaca aaagtgttga gtgcgttggt tatgggaata ttatggctta
    13981 gcatattatt ttgtcgcctt tattttcatg cacattactt ttcagatgtc attggcggca
    14041 cgtcactagc aatcatttgg gtagcgttat tcttaatggt atacccatac tttattaatc
    14101 atcgacgaca acgcgtttag caaacatctt tagacatagt ggaggtatat agaaaaatga
    14161 gaattaaaac accgagtcca tcgtatttaa aaggcacaaa tggacatgcg atattattat
    14221 tacattcatt tacaggtaca aatcgggatg tgaagcatct tgcagctgag ttaaatgacc
    14281 aaggatttag ttgttatgca ccgaattatc caggtcatgg tttattgttg aaagatttca
    14341 tgacatataa tgtagatgat tggtgggaag aagttgagaa agcttaccaa tttttagtca
    14401 atgaaggtta tgaatctatc agtgcaacgg gtgtgtcttt aggtggatta atgacattaa
    14461 aattggcgca acactatcct ttgaaacgta tcgctgtcat gtcagcacca aaggaaaaga
    14521 gtgacgatgg cttaatagaa catttagttt attatagtca acgcatgtcg aatattttaa
    14581 atttagatca gcaagcatcg agtgcgcaat tagcagcaat tgatgattat gaaggtgaaa
    14641 ttacgaagtt tcaacatttt attgatgata tcatgacaaa tttaaatgtt attaaaatgc
    14701 cagctaatat attatttggt ggtaaagatg cgccatccta tgaaacaagt gcacatttta
    14761 tttatgaaca tttaggatca gtagacaaag aattaaatgg tctgaaggat tcgcatcatt
    14821 taatgacgca tggagaaggc agagatattt tagaagaaaa tgttattcgc tttttcaatg
    14881 ctttaacata attgaagatt ttaaaaatta acacttgagt gacgatgtta ttatatttgt
    14941 catttgagtg ttttttgtgg cgtgatagta tagggaactt agctaaagtt aatttgtagt
    15001 gtgatgtcgt ggaaatatta tggagttata ccagaagttg atttgtattg taatgtcgtg
    15061 gaaattttat gggcattgca cctgaagttg ttatgtggcg agatgttggg gatatagatg
    15121 gtgttgtatg tggcgagata ttggcgcacg tatacagcgt tgaaccagaa aatttaatgt
    15181 agcgtaatga caacacatta caagagttga ttatattatg ataaatccag gtgctaccgt
    15241 attaatgaaa caattttgat gcaggtgtat tgcatgttat ttacatacat gttagagtgt
    15301 tacttaaaca cgtagacgct ttaacgcttt aaaggagatg tgaatgatga aaagacaaca
    15361 atcacaatgg aagtcatcaa ctggatttat tttagctagt gcgggttctg caatcggtct
    15421 tggtgccatg tggaaattcc catatatggc agggatttat ggcggcggtg cctttctagc
    15481 tatgttctta atattcacca tttttgttgg gttgccatta ctcattatgg aattcactgt
    15541 tgggaaaatg ggacggacat atacaacaca aatatatagt aaattaactg gtaaaaaatg
    15601 gctcaatatc attggctgga acggtaattt ggcagtgttt attttatttg gcttctatag
    15661 tgttatcggt ggttggattg tcatttacat cggacaagta ttatggcaat tagttatatt
    15721 tcaacgcatc aatcatctcc aagaaatgaa ttttgaagcg gtaatatcaa atccttggtt
    15781 aaccgttcta gggcaaggta tattcatatt cgctacgatg attattgtca tgttaggtgt
    15841 tgaaaaagga ttagaaaagg catcgaaagt tatgatgcca ttgctgtttg tctttttaat
    15901 cgtcattgtg attaagtctt taacattaga tggcgcctta gaaggtgtga aatttatttt
    15961 acaaccaaga gtatcagaga ttactgctga tggcatcttg tttgcgctag gtcaatcatt
    16021 ctttacgtta tcattaggaa ctacaggtat gattacttat gcgagttatg cctctaaaga
    16081 catgacgatt aagtcatcag ctatttctat cgttgttatg aatatctttg tatctgtatt
    16141 ggcaggtcta gctatatttc cggctttaca tagttttggc tatgaaccac aagaagggcc
    16201 tggattatta tttaaagtac tgccaatggt ctttagtcaa atgcatctag gcacattatt
    16261 ctatttggga ttcttagtgc tgttcttatt tgcggcttta acgtcatcta tttctttatt
    16321 agaattaaat gtttctaact tcacgaagaa tgacaataca aaacgtaaaa aagtcgcagt
    16381 gatcggtagt attttagtat ttatcattag tattccagca accttatctt ttggtatctt
    16441 aaaagatgta agattcggtg cgggaacgat ttttgataat atggatttca tcgtttcgaa
    16501 tgtattgatg ccattaggcg cattaggtac tacgcttgtc gtaggacaat tattagataa
    16561 aaaattatta caacaatatt ttggtaaaga tcgatttaga ttattcagtg gttggtatta
    16621 cttaattaag tatgcgatgc ctgtcgttat tattttagtc tttatcgtgc aattatttag
    16681 ttaatatata gaagcatctg acacacaagt atttgtgttg ctcaggtgtt tttttgtttg
    16741 gaattgaaaa tttagtactg gttctgaatt aatcgaaact tagataattg caaaataaca
    16801 aaatgtcgtt tttaattctt atctgaaaag taagaattaa aattgagaca tgaagaaagg
    16861 tttcattcat ttgatcggca tgtcagatga gggtgcatct atgattactt atgatttaat
    16921 tggcaataca ccattagtac tgttagaaca ttatagtgat gataaagtta aaatttatgc
    16981 caagcttgaa caatggaatc ctggaggcag tgttaaagac agactcggga aatatttagt
    17041 agagaaggca attcaagaag ggcgtgtgcg tgcaggtcaa actattgttg aagcgactgc
    17101 tggtaataca ggcatagggt tagctattgc agcgaataga catcatttga aatgtaagat
    17161 ctttgcgccg tatggttttt cagaagaaaa gattaatatt atgatagcgc ttggtgcaga
    17221 agtttcaagg acgagtcagt ctgaaggtat gcatggggca caattagctg cacgttccta
    17281 tgctgaaaaa tatggtgccg tttatatgaa tcaatttgaa tccgaacata atccggatac
    17341 atattttcat acattgggac ccgaattgac ttcagcatta cagcaaattg attattttgt
    17401 ggctggtatt ggctctggcg gtacatttac aggtaccgca cgttatttaa agcaacatca
    17461 cgtgcaatgt tatgccgttg agccagaagg gtccgtgtta aatggagggc cagctcatgc
    17521 acatgacact gaaggtatcg gttctgagaa atggccgata tttttagaga gacgtcttgt
    17581 agatgggata tttacgatta aagatcaaga tgcctttcga aatgtcaaaa gtttggctat
    17641 aaatgaaggg ttgttagtag gcagttcttc aggtgcagca ttacaaggtg cattgaattt
    17701 aaaagcgcaa ttatctgaag gtacgattgt tgtcgtattt ccagatggta gtgatcgata
    17761 tatgtctaag caaatattta attatgagga gaataataat gaacaagaaa actaaattaa
    17821 ttcatggtgg gcacacaaca gacgattata caggtgccgt tacaacacca atttatcaaa
    17881 caagtacata tttacaagat gatattggtg atttacgtca aggatatgaa tattctcgta
    17941 ctgcgaatcc aacaagaagt tctgtagaaa gcgttattgc gacattagaa aatggcaaac
    18001 atggctttgc atttagttca ggtgttgcag caatcagtgc agttgttatg ctgttggaca
    18061 aaggagatca tattatttta aattcagatg tatacggcgg tacttatcgc gcattgacaa
    18121 aagtatttac acgatttggc attgaagtgg attttgtaga tacaacgcat acagattcaa
    18181 ttgtacaagc gatacgccca acaacaaaga tgttgtttat tgaaacacct tctaatccat
    18241 tattacgtgt tactgacatt aaaaagtctg ctgaaattgc gaaagaacac ggtttgattt
    18301 cagttgttga taacacattt atgacacctt attatcagaa tccattagat ttaggtatcg
    18361 atattgtctt acattctgca acgaaatatt taggtggaca tagtgatgtc gttgctggtt
    18421 tagttgcaac atcggatgac aagcttgcag aacgtttagc atttatttca aattcaacag
    18481 gtggcatttt aggacctcaa gatagctatt tacttgtgag gggtattaaa acattaggtt
    18541 tacgtatgga acaaattaat cgcagcgtta ttgaaattat taaaatgtta caagcacatc
    18601 cagctgtgca acaagtgttc catccaagta ttgaaagtca tttaaatcat gatgtccata
    18661 tggctcaagc ggatggccat acaggtgtga ttgcatttga agtgaaaaat acagaaagtg
    18721 ccaaacaatt gattaaagca acatcgtatt acacattagc tgaaagttta ggtgcagtgg
    18781 aaagtttaat ttcagtacct gcattgatga cacatgcatc cattccagca gatattcgag
    18841 ctaaagaagg tattacagac ggacttgtaa gaatttctgt aggtattgaa gatactgaag
    18901 atttagtcga tgatttaaaa caagcactag atactttata aataatagca gcactggcat
    18961 atattttgag ttcaacgttt gttgttcttg aatatgctag tgcttttttg tgcgatgtta
    19021 atatttagta attgcgttgt ggaggattgg tggacatttt tatggattta atttgataaa
    19081 tgtcatagta gtctcacaat tcgtcattgt cacatgaatt acttattttt taatttttta
    19141 gaaaattcgg caaattgtat tgacgatata tgcaatttta catataatgc tcttattcct
    19201 agtggattaa taagaatttg taggaggggc gatgatgatt gagtttcgac aggtaagtaa
    19261 gacctttaat aaaaagaagc aaaaaataga tgctttgaag gacgtatcat ttacggtcaa
    19321 tcgcaatgat atttttggtg tgattggata tagtggtgca ggaaaaagta cgttggtaag
    19381 actcgtgaat catcttgaag ctgcctcgaa tggacaagtg attgtagatg gacatgatat
    19441 tacgaattat agcgataaaa tgatgaggga tattaagaaa gatatcggta tgatatttca
    19501 gcatttcaat ttattaaatt cagctaccgt atttaaaaat gtagcaatgc cactcatttt
    19561 aagtaagaaa agcaaaacag aaattaagca acgagtaacg gaaatgcttg aatttgtagg
    19621 attgagtgat aaaaaagacc aatttcctga tgaattatct ggtgggcaga agcaaagggt
    19681 ggctattgca agagcgcttg ttactaatcc gaaaatactc ctatgcgatg aagcaacaag
    19741 cgcattggat ccagcaacga ctgcttcgat attgacgtta ttaaagaatg tcaatcaaac
    19801 ctttggcatt acaattatga tgattacaca tgaaatgcgc gttattaaag acatttgtaa
    19861 tcgtgttgct gtaatggaaa aggggaaagt ggttgaaaca ggaactgtta aagaggtgtt
    19921 tagtcatcct aaaacgacga ttgctcaaaa ttttgtgtct acagttatac agactgagcc
    19981 aagtacatca ttgattcgtc gattgaatga cgaacaagtt ggcgatttta aagattataa
    20041 aatcttcgtc gaggaaactc aggtgacaca accgattata aatgacttga ttcaaatttg
    20101 tggcagagag gttaaaattt tattttcatc tatgtcagaa atacaaggta acaccgtatg
    20161 ttatatgtgg cttcgattta atatggatca acaatttgaa gacacggcaa taaatcaata
    20221 tttcaaagag aaaaatattc aatttgagga ggtgcattaa catatgtttg gttctgattt
    20281 agacagtgca cagttattac aagcattgta cgaaacgcta tatatggtat ctattgcttt
    20341 atttttagga gcagtgattg gtattccatt aggtgtctta ttggtaatta ctcgaaaaca
    20401 aggcatatgg cccaatatag tgatacatca agttttaaat cctttaatca atattttaag
    20461 gtcactacca tttattattt tgttaattgc gattgtgcca ttcacaaaat tagtagtagg
    20521 tacttcaatt ggtacgactg ctgccatcgt gcctttaaca gtatatgtgg caccttacat
    20581 tgcaagactt gttgaaaact cattattgga agtagacgag gggattattg aagcggcgaa
    20641 agcgatgggc gcttcaccac tacaaatcat tagatatttt ttaattcctg aagctttagg
    20701 ttcgttagta ttagcaatta ccactgcgat tattggactt attggaagta cggcgatggc
    20761 aggagctgtt ggcggtggtg gtataggaga cttagcttta gtgtatggtt atcaaagatt
    20821 tgatacgacg gtcattatta ttaccgttat tgtattagtc attattgtcc aagtgattca
    20881 aacgctaggg aatgttctag ctagattcat acgtagacat taatgatata tagtgaagat
    20941 tttgaaagga attgatagaa tgaaaagatt gattgggtta gttatcgtag cacttgtatt
    21001 attagcagcg tgtggtggta acaatgataa aaaagtaaca attggtgtcg catcaaatga
    21061 cactaaggct tgggagaagg ttaaagaatt agctaaaaaa gatgatattg atgtggagat
    21121 taagcacttc tcagattaca atttaccgaa taaagcatta aatgatggtg atattgatat
    21181 gaatgcattc caacattttg catttttaga tcaatataaa aaggcgcata aaggaacaaa
    21241 gatttcagca ttaagtacaa cagttttagc accgttgggc atttactcag ataaaattaa
    21301 agatgtcaaa aaggttaaag atggtgctaa agttgtcatt ccaaatgatg tgtcaaacca
    21361 agcacgtgca cttaaactat tagaagcagc tggtttaata aaactgaaaa aagatttcgg
    21421 attagcaggc acggtgaaag atataacgtc aaatccaaaa catttaaaaa ttactgcagt
    21481 agatgcacaa caaactgcac gtgctttatc tgatgtcgat attgcagtta ttaataacgg
    21541 tgtagcaact aaagcgggta aagatcctaa aaatgatccg atatttttag aaaaatcaaa
    21601 ttcagatgca gtaaagccat atattaatat tgttgcagtt aatgacaaag acttggataa
    21661 caaaacatat gctaaaatcg tagaattgta tcattcaaaa gaagctcaaa aagcgttgca
    21721 ggaagatgtc aaagatggag agaaacctgt taatttatct aaagatgaga ttaaggcaat
    21781 agaaacgtca ttagcaaaat aaattatatt gcgtcctaca agcaaagttc atgcttatgt
    21841 ttgtagggcg ttattgttgg agaataaaat tatttccaat agagaaaggg attgtaatca
    21901 ttttatagtg aaatattatg aaattgtaat aatttagata ttgtaaaatc taataagttg
    21961 taataatttt aaggggtaat tataaaattt gatgatacag tatatgattt ttttgtaatc
    22021 ataatgtcat caaacatcaa cctattatac ataataaaat cgtataatga tgtagtattc
    22081 ataaattcgg ataaaagaat gttaggaaag ttaagcaaga ggaggatttt aaagtgcaaa
    22141 aaaaagtaat tgcagctatt attgggacaa gcgcgattag cgctgttgcg gcaactcaag
    22201 caaatgcggc tacaactcac acagtaaaac cgggtgaatc agtgtgggca atttcaaata
    22261 agtatgggat ttcgattgct aaattaaagt cattaaacaa tttaacatct aatctaattt
    22321 tcccaaacca agtactaaaa gtatctggct caagtaattc tacgagtaat agtagccgtc
    22381 catcaacgaa ctcaggtggc ggatcatact acacagtaca agcaggcgac tcattatcat
    22441 taatcgcatc aaaatatggt acaacttacc aaaacattat gcgacttaat ggtttaaata
    22501 atttctttat ttatccaggt caaaaattaa aagtatcagg tactgctagc tcaagtaacg
    22561 ctgcgagcaa tagtagccgt ccatcaacga actcaggtgg cggatcatac tatacagtac
    22621 aagcaggtga ctcattgtca ttaattgcat caaaatatgg tacaacttat caaaaaatta
    22681 tgagcttaaa tggcttaaat aatttcttta tatatccggg tcaaaaattg aaagtaactg
    22741 gtaatgcatc tacgaactca ggatctgcaa caacgacaaa tagaggttac aatacaccag
    22801 tattcagtca ccaaaactta tatacatggg gtcaatgtac atatcatgta tttaatcgtc
    22861 gtgctgaaat tggtaaaggt attagtactt attggtggaa tgctaataac tgggataacg
    22921 cagcggcagc agatggttac actatcgaca atagacctac tgtaggttct atcgctcaaa
    22981 cagatgtagg ttactatggt catgttatgt ttgtagaacg tgtaaataac gatggtagta
    23041 ttttagtttc agaaatgaac tattcagctg caccaggtat tttaacttac agaacggtac
    23101 cagcttacca agtaaataat tatagatata ttcactaaag tcttacgtat ataaatatat
    23161 aatgaattcc tattacattt cacaagctga aaagttggat tgaatcctaa attttatggc
    23221 ggggctatca aagtcgtgat tgttgtaata ggaatttttg tatatgaaaa aggattggtc
    23281 gaatgaactt catgttctat gttcgaccaa tccttttctg aattagtatt catgttttac
    23341 tttgcgaatg cttgtaaata atctagcacc gtttgttatt aaagtaacaa ctgccactgc
    23401 ttttttgaac ttacgtggtg acttaattga aatgtaaaag tctaacacgg ttcctacagt
    23461 gcttaaacct aatgaaagat atactaattt tttattttta gcatgatatt tatagccatt
    23521 gtagccgtcg actatgaaac ctgcgacatt tagtaaactt gataaacgtt gtgatttgga
    23581 acgttttgcc ataataataa ttccccctag tatcttctgt ttatatgaaa agttgggtgc
    23641 gctgaattgc taacgttttg cgctataact actcatatat gataacataa ttgtacagta
    23701 taatttgaaa aattgatttc acaaagttgg ggtgtcaaag atgattaaat gtgtctgttt
    23761 agttgaagaa acagctgata aaatattgct tgttcaagta aggaatcgcg aaaagtatta
    23821 tttcccgggt ggtaaaatag aagaagggga atcacaagta cacgcgctgt taagagaagt
    23881 aaaagaagaa ttaaatttaa cattaacaat ggatgaaatt gaatatgtcg ggacaattgt
    23941 aggtcctgca tatccacaac aggatatgtt aactgagtta aatggatttc gcgcattaac
    24001 caaaatcgat tgggaaaacg taactatcaa taatgaaatt acggatatac gctggattga
    24061 taaagataat gatgcgttga ttgcgcctgc tgtcaaagtt tggattgaaa cttatggtgg
    24121 taaacatgac aaataatgac accatcatgt tacgacatta tgtcccacaa gattattcga
    24181 tgttagaagc ttttcaatta agtgaaagtg atttgaagtt tgttaaaacg ccagaggaaa
    24241 atattacagc tgcaatgtct gataatgaaa ggtatcccat cgttgtaatg gatggcaggc
    24301 aatgtgtggc cttttttaca ttacatcgtg gaaaaggggt cgcaccattt agcgataacc
    24361 aagatgcagt atttttcagg tcatttagtg ttgatcaacg ttatcgtaat agaggaatag
    24421 gtaaagtggt aatggaaaaa ttggcgtcat ttatcacttc aacatttcag gatattaatg
    24481 agattgtgtt aacggttaat actgacaatc cacatgccat ggcactttat cgccaacaag
    24541 gatatcaata tatgggagat agtatgttcg tcggaagacc tgttcatatt atggcgttaa
    24601 ctataaaata aattaaattt aaaagcatct ttactcatcg tcgaccacaa caattaatga
    24661 tgaataaagg tgctttttgt tatagatcat cggacaattt actatagtaa aaagcgacct
    24721 agtgaacaat tgacatatat ccacaggtcg cttaacttaa gttatattgc tagttgcgat
    24781 taattgatag actcatcatt tttgcgctgt cgagatggtc tttttattaa aaatgccgta
    24841 atccaagccg taatcggaat actgattgca acggcaatac cgcctaaaat aatagaaata
    24901 aattcttggg caaatatttt cgagtttata atatgaccaa atgaatattt aagtttgaaa
    24961 aaccaaataa ataaagcaag ttggccacca aaaaaggcaa ggtaaatcgt gttcgcagat
    25021 gtcgctaaaa tttctctacc aacacgcatg ccagattgga ataattcgta ttgcgtaagc
    25081 gttggattca cttgatgcaa ttcataaatg ggtgaactaa tggtaattgt taaatctatc
    25141 acagctgcaa taacagcaag aataatagtg aacaccataa attgaaccat atcaatgcca
    25201 atattcattg aatacacata tgtttcatct tgttgttcgg ttgaaaagcc ttgtagatga
    25261 ccgaagtaga ccgataaata aatgagtgta atcaacaata ttgttgtaac gatagtgctg
    25321 ataaatgcag cttgtgtttt aacattgtaa ctattgagta cgaataaatt acaagcgcca
    25381 ataataatgc agaaaaagaa tgtgacgaca taaatcggta cgccaaaaat aatcaataca
    25441 atactaataa ttaaaatagc gaaatttaaa aatagggtta aataagagat gaatcccttt
    25501 ttacctccga aaattatcat cagaaagagg agcaataacg ccaatataaa tacagcattc
    25561 attgtttcgc cctccttaat gtttcaaata tttccataaa caatattgtg ataggaattg
    25621 taagtacgat acctatacca cctgttagtg cgcgcgcaat ttctaacgac caattcatcg
    25681 aaatagtata agtcactgta ttggcatttt ttaaaaagat taaaaacata ggtagtgcac
    25741 cggataaata tgagaataat aagatgttag tcattgttcc cataatatct tggccgatgt
    25801 ttcgcccagc aagcgcccat ctcctcattg aaatgtgtgg cgtacgctgt aaaatttcat
    25861 gcataccact agcaattgta attgcaacat ccataatagc gccaagtgaa cctattaaca
    25921 ctgaggctag gaagatatct ttcggtggta atgataaaaa gttcatcgtt tcatatttaa
    25981 tgcctttacc atctgccata tatatgatta attctgttaa acctatactc aaaaaagttc
    26041 cgataattgt actggctatg gtaatgagtg tacgcatatg ccagcctgta acgagcaata
    26101 aagtgagtat tgttgaacag atcatggcaa tggtcatgag taagaataaa ttaatattgc
    26161 tatgttgaat atgaatgtaa attgcgatta atatggcaat agaattcaag attaacgata
    26221 aaatcgattg cagtccgact ttgcgaccaa ccaataatac agttaataag aacaaaccag
    26281 tgatgataac cgttaaggta tcacgcttct tttctataat ataagcatca ctcggcttgt
    26341 tagaaatatg taataatact ttttcgtgtg tgcgaaatgc ctcagaatct gcttgcgatt
    26401 tgacgtactg atgattaatc gtcgtcgttt ctccagcaaa ttgaccattt aatattttga
    26461 cttttaattg atttttatat ttaatatcac gattattttg tgcatctttt gtaggtgtcg
    26521 aagaaacatg tttgacatct ataatttgac caattggttt gttgtaaaag ttctcattat
    26581 tgaatgtaaa taaaatagca ccaatgaatg cgatgcagaa caaacctaaa attatattaa
    26641 atggctttgt aaataaattt ctatatt
//
LOCUS       DAKTIP010000014        26428 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAKTIP010000014 DAKTIP010000000
VERSION     DAKTIP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 26428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.014"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            5..80
                     /locus_tag="O0305_000467"
     tRNA            5..80
                     /locus_tag="O0305_000467"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Val,seq:tac)
     gene            97..172
                     /locus_tag="O0305_000468"
     tRNA            97..172
                     /locus_tag="O0305_000468"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Thr,seq:tgt)
     gene            178..253
                     /locus_tag="O0305_000469"
     tRNA            178..253
                     /locus_tag="O0305_000469"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Lys,seq:ttt)
     gene            257..338
                     /locus_tag="O0305_000470"
     tRNA            257..338
                     /locus_tag="O0305_000470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:291..293,aa:Leu,seq:tag)
     gene            342..416
                     /locus_tag="O0305_000471"
     tRNA            342..416
                     /locus_tag="O0305_000471"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Gly,seq:gcc)
     gene            427..515
                     /locus_tag="O0305_000472"
     tRNA            427..515
                     /locus_tag="O0305_000472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:461..463,aa:Leu,seq:taa)
     gene            534..607
                     /locus_tag="O0305_000473"
     tRNA            534..607
                     /locus_tag="O0305_000473"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:568..570,aa:Arg,seq:acg)
     gene            617..690
                     /locus_tag="O0305_000474"
     tRNA            617..690
                     /locus_tag="O0305_000474"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:651..653,aa:Pro,seq:tgg)
     gene            708..783
                     /locus_tag="O0305_000475"
     tRNA            708..783
                     /locus_tag="O0305_000475"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:741..743,aa:Ala,seq:tgc)
     gene            805..878
                     /locus_tag="O0305_000476"
     tRNA            805..878
                     /locus_tag="O0305_000476"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Met,seq:cat)
     gene            907..980
                     /locus_tag="O0305_000477"
     tRNA            907..980
                     /locus_tag="O0305_000477"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:941..943,aa:Ile,seq:cat)
     gene            990..1079
                     /locus_tag="O0305_000478"
     tRNA            990..1079
                     /locus_tag="O0305_000478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1026..1028,aa:Ser,seq:tga)
     gene            1090..1165
                     /locus_tag="O0305_000479"
     tRNA            1090..1165
                     /locus_tag="O0305_000479"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1123..1125,aa:Asp,seq:gtc)
     gene            1204..1296
                     /locus_tag="O0305_000480"
     tRNA            1204..1296
                     /locus_tag="O0305_000480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1240..1242,aa:Ser,seq:tga)
     gene            1311..1384
                     /locus_tag="O0305_000481"
     tRNA            1311..1384
                     /locus_tag="O0305_000481"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1345..1347,aa:Met,seq:cat)
     gene            1394..1469
                     /locus_tag="O0305_000482"
     tRNA            1394..1469
                     /locus_tag="O0305_000482"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1427..1429,aa:Asp,seq:gtc)
     gene            1488..1560
                     /locus_tag="O0305_000483"
     tRNA            1488..1560
                     /locus_tag="O0305_000483"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1521..1523,aa:Phe,seq:gaa)
     gene            1568..1643
                     /locus_tag="O0305_000484"
     tRNA            1568..1643
                     /locus_tag="O0305_000484"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1601..1603,aa:Thr,seq:tgt)
     gene            1649..1729
                     /locus_tag="O0305_000485"
     tRNA            1649..1729
                     /locus_tag="O0305_000485"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Tyr,seq:gta)
     gene            1745..1818
                     /locus_tag="O0305_000486"
     tRNA            1745..1818
                     /locus_tag="O0305_000486"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1777..1779,aa:Trp,seq:cca)
     gene            1821..1893
                     /locus_tag="O0305_000487"
     tRNA            1821..1893
                     /locus_tag="O0305_000487"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1854..1856,aa:His,seq:gtg)
     gene            1905..1976
                     /locus_tag="O0305_000488"
     tRNA            1905..1976
                     /locus_tag="O0305_000488"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1937..1939,aa:Gln,seq:ttg)
     gene            1982..2055
                     /locus_tag="O0305_000489"
     tRNA            1982..2055
                     /locus_tag="O0305_000489"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2014..2016,aa:Cys,seq:gca)
     gene            2063..2137
                     /locus_tag="O0305_000490"
     tRNA            2063..2137
                     /locus_tag="O0305_000490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2096..2098,aa:Gly,seq:tcc)
     gene            2241..2315
                     /locus_tag="O0305_000491"
     tRNA            2241..2315
                     /locus_tag="O0305_000491"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2274..2276,aa:Gly,seq:tcc)
     gene            2354..2437
                     /locus_tag="O0305_000492"
     tRNA            2354..2437
                     /locus_tag="O0305_000492"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2388..2390,aa:Leu,seq:caa)
     gene            complement(2441..2554)
                     /locus_tag="O0305_000493"
     CDS             complement(2441..2554)
                     /locus_tag="O0305_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273652.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKETLFYKVSNTLCYVNNS"
     gene            complement(2530..3891)
                     /locus_tag="O0305_000494"
     CDS             complement(2530..3891)
                     /locus_tag="O0305_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCY0273653.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLP
                     EHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVK
                     TVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYT
                     IFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLR
                     KDLRLCNWPKFINRLNSVSKKSVSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGI
                     NNKIKLIKRISFGYRNFNNFKARIMMIFSLYKGEKRRQPSPIMDWPPNNKNSKSCILK
                     IVL"
     gene            4117..5025
                     /gene="rocF"
                     /locus_tag="O0305_000495"
     CDS             4117..5025
                     /gene="rocF"
                     /locus_tag="O0305_000495"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCY0273654.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            5214..6023
                     /locus_tag="O0305_000496"
     CDS             5214..6023
                     /locus_tag="O0305_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCY0273655.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            6025..6957
                     /locus_tag="O0305_000497"
     CDS             6025..6957
                     /locus_tag="O0305_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCY0273656.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            6984..8339
                     /locus_tag="O0305_000498"
     CDS             6984..8339
                     /locus_tag="O0305_000498"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCY0273657.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            8616..16052
                     /locus_tag="O0305_000499"
                     /pseudo
     CDS             8616..16052
                     /locus_tag="O0305_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(16371..17477)
                     /locus_tag="O0305_000500"
     CDS             complement(16371..17477)
                     /locus_tag="O0305_000500"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCY0273658.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVEDSSIEAGLRRVLAETSQYITNEFDFTEAEQAAYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(17477..17911)
                     /locus_tag="O0305_000501"
     CDS             complement(17477..17911)
                     /locus_tag="O0305_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0273659.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(17923..20055)
                     /locus_tag="O0305_000502"
     CDS             complement(17923..20055)
                     /locus_tag="O0305_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0273660.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLDQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMI
                     DKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRNKFLERIKKQFI"
     gene            complement(20090..21628)
                     /locus_tag="O0305_000503"
     CDS             complement(20090..21628)
                     /locus_tag="O0305_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCY0273661.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            22309..24114
                     /gene="glmS"
                     /locus_tag="O0305_000504"
     CDS             22309..24114
                     /gene="glmS"
                     /locus_tag="O0305_000504"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCY0273662.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            24346..25128
                     /locus_tag="O0305_000505"
     CDS             24346..25128
                     /locus_tag="O0305_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273663.1"
                     /translation="MLIQLDQIGRMKQGKTILKKISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATSGTVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDDEIRNEAHQLLKLVGMFAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPTLAMIYVTHFIEEITANFSKILLLK
                     DGQSIQQGAVEDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            25196..26053
                     /locus_tag="O0305_000506"
     CDS             25196..26053
                     /locus_tag="O0305_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0273664.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAEHFLAE"
BASE COUNT         9431 a         4399 c         4872 g         7726 t
ORIGIN      
        1 aatcggagaa ttagctcagc tgggagagca tctgccttac aagcagaggg tcggcggttc
       61 gaacccgtca ttctccacca tttattctta catattgccg gcctagctca attggtagag
      121 caactgactt gtaatcagta ggttgggggt tcaagtcctc tggccggcac catggaagag
      181 ccattagctc agttggtaga gcatctgact tttaatcaga gggtcagagg ttcgaatcct
      241 ctatggctca ccatttgcgg gtgtggcgga attggcagac gcactagact taggatctag
      301 cgccttacgg cgtgggggtt cgactccctt cacccgcata tgcagaagta gttcagcggt
      361 agaatacaac cttgccaagg ttggggtcgc gggttcgaat cccgtcttct gctccatttt
      421 tatagtgccg gggtggcgga actggcagac gcacaggact taaaatcctg cggtgagtga
      481 tcaccgtacc ggttcgattc cggtcctcgg caccattttc aataaaaaca tatgcgcccg
      541 tagctcaatt ggatagagcg tttgactacg gatcaagagg ttatgggttc gactcctatc
      601 gggcgcgtta attatacggg aagtagctca gcttggtaga gcacttggtt tgggaccaag
      661 gggtcgcagg ttcgaatcct gtcttcccga tatactgtaa ttattatggg ggcttagctc
      721 agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctagtctcca
      781 ccatattatt tacaaactat ataaggcggt gtagctcagc tggctagagc gtacggttca
      841 tacccgtgag gtcgggggtt cgatcccctc caccgccact atttattagt tgtaaaatta
      901 tatttaggac ctttagctca gttggttaga gctaacggct cataaccgtt cggtcgcagg
      961 ttcgagtcct gcaaggtcca tataattttg gaggaatacc caagtccggc tgaagggatc
     1021 ggtcttgaaa accgacaggg gcttaacggc tcgcgggggt tcgaatccct cttcctccgt
     1081 tttactaatg gtctcgtagt gtagcggtta acacgcctgc ctgtcacgca ggagatcgcg
     1141 ggttcgattc ccgtcgagac cgccatttaa ttttataatt aatagcgatt tacctataat
     1201 aatggaggaa tacccaagtc cggctgaagg gatcggtctt gaaaaccgac agggccttaa
     1261 cgggccgcgg gggttcgaat ccctcttcct ccgccattat tttttattat cgcgggatgg
     1321 agcagttcgg tagctcgtcg ggctcataac ccgaaggtcg gtggttcaaa tccgcctccc
     1381 gcaatatttt ataggtctcg tagtgtagcg gttaacacgc ctgcctgtca cgcaggagat
     1441 cgcgggttcg attcccgtcg agaccgccat cattacattt ttattatggt tcagtagctc
     1501 agttggtaga gcaatggatt gaagctccat gtgtcggcag ttcgactctg tcctgaacca
     1561 tttcttagcc ggcctagctc aattggtaga gcaactgact tgtaatcagt aggttggggg
     1621 ttcaagtcct ctggccggca ccatttatgg aggggtagcg aagtggctaa acgcggcgga
     1681 ctgtaaatcc gctccttcgg gttcggcagt tcgaatctgc ccccctccat ttattatttt
     1741 taataggggc atagttcaac ggtagaatag aggtctccaa aacctttggt gtgggttcga
     1801 ttcctactgc ccctgccatg gcggctgtgg tgaagtggtt aacacatcgg attgtggttc
     1861 cgacattcga gggttcgatc cccttcagcc gcccttatta ttaatgggct atagccaagc
     1921 ggtaaggcaa cggactttga ctccgtcact cgttggttcg aatccagcta gcccagttat
     1981 tggcggcata gccaagtggt aaggcagagg tctgcaaaac ctttatcacc ggttcaaatc
     2041 cggttgccgc ctccaggttt atgcgggagt agttcaactt ttagaacacg ttccttcccg
     2101 gaacgaggta taggtgcaaa tcctatcttc cgctccataa tttaataata atgcgggagt
     2161 atttcaactc ttagaataca ttccttcctg gaatgaggta taggtgtaaa tcctatcttc
     2221 cgctccataa tttaatattt gcgggagtag ttcaactttt agaacacgtt ccttcccgga
     2281 acgaggtata ggtgtaaatc ctatcttccg ctccataatt gcttccaaag ggaagttttt
     2341 tgtttaccat taagccggtg tggcggaatt ggcagacgcg cgggactcaa aatcccgttc
     2401 cacttgtgga gtgtcggttc gaccccgacc accggtataa ttaactgtta tttacataac
     2461 ataacgtatt agaaaccttg taaaacaagg tttcttttgg ttctccacca aatgtggtgg
     2521 gtatataatt taaagaacta tttttaaaat acaactttta gagtttttat tattaggcgg
     2581 ccagtccatt attgggcttg gttgtcttct tttttctcct ttgtacaagc tgaaaatcat
     2641 cattatacgt gctttaaagt tgttgaaatt tctgtaacca aaagaaattc gcttgattaa
     2701 ttttatctta ttattaattc cttctatagc accattatta aatgctgggt aataaattgt
     2761 atttcttaac atcctttgat gttttctata atatttaacc gctttccata cacccttact
     2821 cacagacttt ttactaactg aatttaaacg attaataaat ttaggccaat tacataacct
     2881 taggtctttt cgtaatcctt ggacaagttc gtaggagtgt cgtagtatat cgtcttttga
     2941 aagcatgaat tctacaatgt cagatgagcg tttataagcc ttaaaagatt tattccatct
     3001 gtatttacta aatatggttt tactagtatc catcaatagg actttccagt tattcttaaa
     3061 aattgtataa tcaggtcctt ttttattacg gtattcattc ataacttgta cacgatactt
     3121 attaagttct ctatttaaat gttgaacgat atggaatctg tcaaaaataa tagctgcatt
     3181 aggaaatagg tcgcgaaata atcggacata gggttcatac atgtcaatag taaccgtctt
     3241 aacttgttgt cgatttttta aatcgaatcg ctcgaaatag gcacgcaaga atcttgtagt
     3301 tctattttct aaaatatcta taacatcatg agtatcatta tctataaaaa tgaaactcat
     3361 tgatccagtt acatttttaa cgcttttaaa ttcatccata gcgatgtgtt ctggcaaaca
     3421 attaaagggt ttaattcgta atgaattagc cgctttagtt ctaatacgac ttacagtaga
     3481 tggagagata cagttatctt ctgcagtatc aatctctgtt ttaactttag tagcttcttc
     3541 tataactttt tgagtaacac atcttgagat aaaacacgac tcatcaacaa ttgacgtttt
     3601 agcagtaaaa gtgccattgc attctaagca tttaaaacgt tgtttagcta agttgaggta
     3661 tgtgttatac ccttgagttt taagtaaagt aatacgagaa acacgcttac catgtttatg
     3721 aatttgtcct tcatttttaa ctccacaaca ataacaggcc gaaggcttat acgttagtac
     3781 agcattaacc actgttgctt tttttccttt gataatgacg tcaatttctt ggtttatata
     3841 ttttatattt tcatctttta ttcttagtaa ttctaaggta tcattacaca taggcgcact
     3901 tgtctccttt agtttgggtt tggtcacttt aaattataga ggcaattgcg ctttttgtgt
     3961 attaaaaaaa gcaggaagtt ttaccttccc accataaaag atgaagaacc tttttttatg
     4021 aagtaataag ataaacagat tatatgtgta gctattgctt gataatggta agcgcataca
     4081 ttattcttta attacataga gcaaaggggg acgcttatga caaagacaaa agcaattgat
     4141 attataggtg caccatcaac atttggacaa agaaaattag gtgttgattt aggaccaaca
     4201 gcaattagat atgctggatt aatttcaaga ttaaagcaat tagaccttga tgtatatgac
     4261 aagggggata ttaaggtacc tgctgtgaac attgaaaaat ttcatagtga acaaaaagga
     4321 ttaagaaatt atgatgaaat tatagatgtt aatcaaaaat taaataaaga ggtttcagca
     4381 tcaattgaaa ataacagatt tcctctagtt cttggtggag atcattctat tgcggtaggt
     4441 tcagtatcag caataagtaa acattataat aatttaggtg ttatttggta tgatgcacat
     4501 ggtgatttaa atatacctga agagtcacca agtggaaata ttcatggtat gcctctaagg
     4561 attttgacag gcgaaggtcc caaagaactt ttagaattaa atagtaatgt aatcaagcca
     4621 gaaaacatcg tactaattgg tatgagagat ttagataaag gtgaaagaca atttatcaaa
     4681 gatcataata ttaaaacatt tactatgtca gatattgata aattggggat aaaggaagta
     4741 attgaaaata caatagaata tttgaagtca cgcaatgttg atggcgttca tttatcttta
     4801 gatgttgatg ctttagatcc gcttgaaacg ccaggcactg gtactagagt tttgggtggt
     4861 cttagttata gagaaagcca ttttgcattg gaattactgc atcaatcaca tttaatttcc
     4921 tcaatggatt tagttgaagt aaatccattg attgacagta ataatcatac tgctgaacaa
     4981 gcggtttcat tagttggaac attttttggt gaaactttat tataaataaa tgatttgtag
     5041 tgtataaagt atattttgct ttttgcacta ctttttttaa ttcactaaaa tgattaagag
     5101 tagttataat ctttaaaata aattttttct atttaaatat atgttcgtat gacagtgatg
     5161 taaatgattg gtataatggg tattatggaa aaatattacc cggaggagat gttatggatt
     5221 tttccaactt ttttcaaaac ctcagtacgt taaaaattgt aacgagtatc cttgatttac
     5281 tgatagtttg gtatgtactt tatcttctca tcacggtctt taagggaact aaagcgatac
     5341 aattacttaa agggatatta gtaattgtta ttggtcagca gataagtatg atattgaact
     5401 tgactgcaac atctaaatta ttcgatatcg ttattcaatg gggggtatta gctttaatag
     5461 taatattcca accagaaatt agacgtgcgt tagaacaact tggtagaggt agctttttaa
     5521 aacgctatac ttctaatacg tatagtaaag atgaagagaa attgattcaa tcggtttcaa
     5581 aggctgtgca atatatggct aaaagacgta taggtgcatt aattgtcttt gaaaaagaaa
     5641 caggtcttca agattatatt gaaacaggta ttgcaatgga ttcaaatatt tcgcaagaac
     5701 ttttaattaa tgtctttata cctaacacac ctttacatga tggtgcaatg attattcaag
     5761 gcacgaagat tgcagcagca gcaagttatt tgccattgtc tgatagtcct aagatatcta
     5821 aaagtttggg tacaagacat agagctgcgg ttggtatttc agaagtatct gatgcattta
     5881 ccgttattgt atctgaagaa actggtgata tttcggtaac atttgatgga aaattacgac
     5941 gagacatttc aaacgaaatt tttgaagagt tgcttgctga acattggttt ggcacacgct
     6001 ttcaaaagaa aggtgtgaaa taatatgcta gaaagtaaat ggggcttgag atttattgcc
     6061 tttcttttgg cattgttttt ctttttatct gttaacaatg tttttggaaa tatctttaac
     6121 actggtaatc ttggtcaaaa gtctagtaaa acgattcaag atgtacccgt tgaaattctt
     6181 tataacacta aagatttgca tttaacaaaa gcgcctgaaa cagttaatgt gactatttca
     6241 ggaccacaat caaagataat aaaaattgaa aatccagaag atttaagagt agtgattgat
     6301 ttatcaaatg ctaaagctgg aaaatatcaa gagaagtatc aagttaaagg gttagctgat
     6361 gacattcatt attctgtaaa acctaaatta gcaaatatta cgcttgaaaa caaagtaact
     6421 aaaaagatga cagttcaacc tgatgtaagt cagagtgata ttgatccact ttataaaatt
     6481 acaaagcaag aagtttcacc acaaacagtt aaagtaacag gtggagaaga acaattgaat
     6541 gatatcgctt atttaaaagc cacttttaaa actaataaaa agattaatgg tgacacaaaa
     6601 gatgtcgcag aagtaacggc ttttgataaa aaactgaata aattaaatgt atcgattcaa
     6661 cctaatgaag tgaatttaca agttaaagta gagcctttta gcaaaaaggt taaagtaaat
     6721 gttaaacaga aaggtagttt agcagatgat aaagagttaa gttcgattga tttagaagat
     6781 aaagaaattg aaatcttcgg tagtcgagat gacttacaaa atataagcga agttgatgca
     6841 gaagtagatt tagatggtat ttcagaatca actgaaaaga ctgtaaaaat caatttacca
     6901 gaacatgtca ctaaagcaca accaagtgaa acgaaggctt atataaatgt aaaataaata
     6961 gctaaattaa aggagagtaa acaatgggaa aatattttgg tacagacgga gtaagaggtg
     7021 tcgcaaacca agaactaaca cctgaattgg catttaaatt aggaagatac ggtggctatg
     7081 ttctagcaca taataaaggt gaaaaacacc cacgtgtact tgtaggtcgc gatactagag
     7141 tttcaggtga aatgttagaa tcagcattaa tagctggttt gatttcaatt ggtgcagaag
     7201 tgatgcgatt aggtattatt tcaacaccag gtgttgcata tttaacacgc gatatgggtg
     7261 cagagttagg tgtaatgatt tcagcctctc ataatccagt tgcagataat ggtattaaat
     7321 tctttggatc agatggtttt aaactatcag atgaacaaga aaatgaaatt gaagcattat
     7381 tggatcaaga aaacccagaa ttaccaagac cagttggcaa tgatattgta cattattcag
     7441 attactttga aggggcacaa aaatatttga gctatttaaa atcaacagta gatgttaact
     7501 ttgaaggttt gaaaattgct ttagatggtg caaatggttc aacatcatca ctagcgccat
     7561 tcttatttgg tgacttagaa gcagatactg aaacaattgg atgtagtcct gatggatata
     7621 atatcaatga gaaatgtggc tctacacatc ctgaaaaatt agctgaaaaa gtagttgaaa
     7681 ctgaaagtga ttttgggtta gcatttgacg gcgatggaga cagaatcata gcagtagatg
     7741 agaatggtca aatcgttgac ggtgaccaaa ttatgtttat tattggtcaa gaaatgcata
     7801 aaaatcaaga attgaataat gacatgattg tttctactgt tatgagtaat ttaggttttt
     7861 acaaagcgct tgaacaagaa ggaattaaat ctaataaaac taaagttggc gacagatatg
     7921 tagtagaaga aatgcgtcgc ggtaattata acttaggtgg agaacaatct ggacatatcg
     7981 ttatgatgga ttacaataca actggtgatg gtttattaac tggtattcaa ttagcttctg
     8041 taataaaaat gactggtaaa tcactaagtg aattagctgg acaaatgaaa aaatatccac
     8101 aatcattaat taacgtacgc gtaacagata aatatcgtgt tgaagaaaat gttgacgtta
     8161 aagaagttat gactaaagta gaagtagaaa tgaatggaga aggtcgaatt ttagtaagac
     8221 cttctggaac agaaccatta gttcgtgtca tggttgaagc agcaactgat gaagatgctg
     8281 aaagatttgc acaacaaata gctgatgtgg ttcaagataa aatgggatta gataaataaa
     8341 tactgtatta caaatgagcc gatgcgtatg catcggtttt ttgtgtttgt agaaataatt
     8401 tatagtacaa acgtaaaatg atataaacaa aataaaaaca aagtaatcaa tatgtaatat
     8461 aaaatacact ggtactcaat atataatgat gataaaatta attttaatta gatagagttg
     8521 ctttgtgttt ttaacgcaga tgctactact tatcttaaca gttgattaag tgaaatcatt
     8581 taacagcgag aataatcaac caggaggatg acttaatgaa tttattcaga caacaaaaat
     8641 ttagtatcag aaaatttaat gtcggtattt tttcagcttt aattgccact gttactttta
     8701 tatctactaa cccgacaaca gcgtctgcag cagagcaaaa tcagcctgca caaaatcaac
     8761 cagcacaacc agctgatgcc aatacacagc ctaacgcaaa tgctggtgct caagctaatc
     8821 ctacagcaca gccagctgca cctgccaacc aaggacaacc agcagtacaa ccagcaaacc
     8881 aaggtggaca ggctaatcca gcaggaggag cagcacaacc aaatacacaa ccagctggac
     8941 aaggtaatca agctgatccg aataacgctg cacaagcaca acctggaaat caagcaacac
     9001 cggcaaacca agcaggtcaa ggaaataacc aagcaacacc taataataat gcaacaccgg
     9061 caaatcaaac acagccagcg aatgctccag cagcagcgca accagcagca cctgtagcag
     9121 caaacgcaca aactcaagat ccaaatgcta gcaatactgg tgaaggcagt attaatacga
     9181 cattaacatt tgatgatcct gccatatcaa cagatgagaa tagacaagat ccaactgtaa
     9241 ctgttacaga taaagtaaat ggttattcat taattaacaa cggtaagatt ggtttcgtta
     9301 actcagaatt aagacgaagc gatatgtttg ataagaataa ccctcaaaac tatcaagcta
     9361 aaggaaacgt ggctgcatta ggtcgtgtga atgcaaatga ttctacagat catggtaact
     9421 ttaacggtat ttcaaaaact gtaaatgtaa aaccagattc agaattaatt attaacttta
     9481 ctactatgca aacgaatagt aagcaaggtg caacaaattt agttattaaa gatgctaaga
     9541 aaaatactga attagcaact gtaaatgttg ctaagactgg tactgcacat ttatttaaag
     9601 taccaactga tgctgatcgt ttagatttac aatttattcc tgacaataca gcagttgctg
     9661 atgcttcaag aattacaaca aataaagatg gttataaata ctattcattc attgataatg
     9721 taggtctatt ctcaggatca catttatatg tcaaaaatag agatttagca ccgaaagcaa
     9781 ctaacaataa agaatatact attaatactg aaatcggtaa caatggtaat tttggtgctt
     9841 cattaaaagc agatcaattt aaatatgaag taacattacc acaaggtgta acttacgtta
     9901 ataattcatt aactacaaca ttccctaatg gtaatgaaga cagtacagta ttgaaaaata
     9961 tgactgttaa ttatgatcaa aatgcaaata aagttacatt tacaagccaa ggtgtgacaa
    10021 cggcacgtgg tacacacact aaagaagttt tattcccaga taaatcttta aaattatcat
    10081 ataaagttaa tgttgcgaat atcgatacac ctaaaaatat tgattttaat gaaaaattaa
    10141 catatcgtac tgcttcagat gttgtaatta ataatgcgca accagaagtt acactaactg
    10201 cagatccatt ttcagtagcg gttgaaatga acaaagatgc gttgcaacaa caagtaaact
    10261 cacaagttga taatagtcat tacacaacag catcaattgc agaatacaat aaacttaaac
    10321 aacaagcaga tactatttta aatgaagatg cgaatcatgt taaaactgca aatcgtgcat
    10381 ctcaagcgga tattgatggt ttagtaacta aattacaagc tgcattaatt gataatcaag
    10441 cagcaattgc tgaattagat actaaagctc aagaaaaggt tacagcagca caacaaagta
    10501 aaaaagttac gcaagatgaa gttgcagcac ttgtaactaa aattaacaat gataaaaata
    10561 atgcaatcgc tgaaattaat aaacaaacta cagcacaagg tgtcacaact gaaaaagata
    10621 atggtatcgc agtgttagaa caagatgtga ttacaccaac agttaaacct caagcgaaac
    10681 aagatattat ccaagcagtt acaactcgta aacaacaaat taaaaagtca aatgcatcat
    10741 tacaagatga aaaagatgta gcaaatgata aaattggtaa aattgaaaca aaggcaatta
    10801 aagatattga tgcagcaaca acaaatgcac aagtagaagc cattaaaaca aaagcaatca
    10861 atgatattaa tcaaactaca cctgctacaa cagctaaagc agcagctctt gaagaatttg
    10921 acgaagttgt tcaagcacaa attgatcaag cacctttaaa tcctgataca acaaatgaag
    10981 aagtagcgga agctattgaa cgtattaatg cagctaaagt ttctggtgtt aaagcaattg
    11041 aagcgacaac gactgcacaa gatttagaaa gagttaaaaa cgaagaaatc tcaaaaattg
    11101 aaaatattac tgactctacg caaacaaaaa tggatgccta taatgaagtt aaacaagctg
    11161 caacagctag aaaagctcaa aatgctacag tttcaaatgc aacaaatgaa gaagtagcag
    11221 aagctgatgc agcagtagat gcagctcaaa agcaaggttt acatgacatc caagttgtta
    11281 aatcaaaaca ggaagttgct gatacaaaat caaaagtatt agataaaatc aatgcaattc
    11341 aaacacaagc aaaagttaaa cctgcagctg atacggaagt agaaaacgca tataatacac
    11401 gtaaacaaga aattcaaaat agcaatgctt caactacaga agaaaaacaa gctgcatata
    11461 cagaattaga tactaaaaag caagaagcaa gaacaaatct tgatgctgca aatacaaaca
    11521 gtgatgtaac aacagctaaa gacaatagta ttgctgcaat taatcaagtt caagctgcca
    11581 caactaagaa atcggatgca aaagcggaaa tcgctcaaaa agcaagtgaa cgtaaaacag
    11641 caattgaagc aatgaatgat tcgactactg aagaacaaca agcagcgaaa gacaaagtgg
    11701 atcaagcagt agttactgca aacgctgata tagataatgc tgcagcaaac aatgatgtgg
    11761 ataatgcaaa aactacaaat gaagctacaa tcgcagccat tacacctgat gcaaatgtta
    11821 aaccagcagc aaaacaagca attgcagata aagtacaagc tcaagaaaca gcaattgatg
    11881 gaaataacgg ctcaacaact gaagaaaaag cagctgctaa ataacaagtt caaactgaaa
    11941 aaacaacagc tgatgccgca atagatgcag cacatacaaa tgcggaagtt gaagcggcta
    12001 aaaaagcagc aattgctaaa attgaagcga ttcagccagc aacaacaact aaagataatg
    12061 cgaaagaagc aattgctacg aaagcgaatg aacgtaaaac agcaatcgct caaacgcaag
    12121 acattactgc tgaagaaatt gcagcggcta atgcggacgt agataatgct gtgacacaag
    12181 caaatagcaa cattgaagct gctaatagtc aaaatgatgt agaccaagcg aaaacgacag
    12241 gtgaaaatag tattgatcaa gtaacaccaa cagttaataa aaaagcaact gcacgtaatg
    12301 aaatcacagc aattttaaat aacaaattgc aagagattca agctacgcca gatgcaacag
    12361 atgaagaaaa acaagcagct gatgctgaag caaatactga aaatggtaaa gcaaatcaag
    12421 ccatttcagc agcaactact aacgcacaag ttgatgaagc taaagcaaat gcagaagcag
    12481 cgattaatgc ggtaacacca aaagttgtga agaaacaagc ggctaaagat gaaattgatc
    12541 aattacaagc aacgcaaaca aatgttatca ataatgatca gaacgctaca acagaagaaa
    12601 aagaagcagc tattcaacaa ttagcaacag cagttacaga cgcgaaaaat aatattacag
    12661 ctgcaactga tgataatggt gtagatcagg cgaaagacgc tggaaagaat tcaattcaaa
    12721 gcacgcaacc agcaacagcg gttaaatcaa atgctaaaaa tgatgttgat caagctgtga
    12781 caactcaaaa tcaagcaatt gataatacaa ctggtgctac aactgaagag aaaaatgcag
    12841 caaaagattt agttttaaaa gctaaagaaa aagcgtatca agatatctta aatgcacaaa
    12901 caactaatga tgttacgcaa attaaagatc aagcagttgc tgatattcaa ggtattactg
    12961 cagatacaac aattaaagat gttgcgaaag atgaattagc aacaaaagca aacgaacaaa
    13021 aagcgcttat tgcacaaact gcagatgcga ctactgaaga aaaagaacaa gcaaatcaac
    13081 aagtagacgc acaattaaca caaggtaatc aaaatattga aaatgcacag tcaatcgatg
    13141 atgtaaacac tgcaaaagat aatgcaattc aagcaattga cccaattcaa gcatcaacag
    13201 atgttaaaac gaatgcaaga gcggaattgc taactgaaat gcaaaataaa ataactgaaa
    13261 tacttaataa taatgagact actaatgaag aaaaaggtaa cgatattgga ccagttagag
    13321 cagcatatga agaaggttta aataatatta atgcagcaac tactacaggt gatgtaacta
    13381 ctgctaaaga tacagcagta caaaaagttc aacaacttca tgcaaatcct gttaagaaac
    13441 cagcaggtaa aaaagaatta gatcaagctg cagctgataa gaaaacacaa atagaacaaa
    13501 caccaaatgc atcacaacaa gaaattaatg atgcaaaaca agaagttgat actgaattaa
    13561 atcaagcgaa aacaaatgtc gatcaatcat caacaaatga atatgttgat aatgcagtta
    13621 aagaaggaaa agctaaaatt aatgcagtta aaacatttag tgagtacaaa aaagatgctt
    13681 tagctaaaat tgaagatgca tataatgcta aagtaaacga agcggataac tctaacgcat
    13741 cgacttcaag tgaaattgct gaagcgaaac aaaaacttgc tgaattaaaa caaactgcgg
    13801 atcaaaatgt taatcaagct acttctaaag atgacattga agttcaaatt cataatgact
    13861 tagataatat taacgattac acaattccaa caggtaaaaa agaatcagct acaacagatt
    13921 tatatgctta tgcagatcag aagaaaaata atatttcagc tgacactaat gcaacacaag
    13981 atgaaaagca acaagcaatt aagcaagttg accaaaatgt tcaaactgca ttagaaagca
    14041 ttaataatgg tgtggataat ggtgacgttg atgatgcatt aacacaaggt aaagcagcaa
    14101 ttgatgctat tcaagtagat gctactgtta aacctaaagc gaaccaagct attgaagtta
    14161 aagcagaaga tacgaaagaa tctattgatc aaagtgacca gttaactgct gaagaaaaaa
    14221 ctgaagcatt agcaatgatt aaacaaatta cagatcaagc taaacaaggt attactgatg
    14281 caacaacaac tgctgaagtt gaaaaagcga aagctcaagg acttgaagca tttgataaca
    14341 ttcaaatcga ctcaacagaa aaacaaaaag ctatcgaaga attagaaact gcactagacc
    14401 agattgaagc aggtgtaaat gtcaacgctg atgctacaac tgaagaaaaa gaagcgttta
    14461 cgaatgcttt agaagacatt ttatcaaaag caactgaaga tatttctgat caaactacaa
    14521 atgcagaaat cgctactgtc aaaaatagtg cgcttgaaca acttaaagca caacgtatta
    14581 atcctgaagt taagaaaaat gctttggaag caatcagaga agtggttaac aagcaaatag
    14641 aaataattaa aaatgcagat gcagatgcat cggcgaaaga aattgcacgt acggatttag
    14701 gtagatattt tgacagattt gctgataaat tagataaaac acaaacaaat gcagaagttg
    14761 ctgaattaca aaatgtaacg atacctgcaa ttgaagcgat tgtgcctcag aatgatccag
    14821 atgctaatga tactaataat ggtatagata ataatgatgc aacagcaaat tcaaatgcta
    14881 atgcaacacc agaaaatact ggacaaccta acgtatctga gacgacagct aatggcaaag
    14941 cagacgcttc tccgacaaca ccaaataata gtgatgcggc aacaggtgaa acaacagcaa
    15001 catcagcaac tgatgatgca aatgataaac cgcaagctaa taacaattct agtgtagatg
    15061 catcaacaaa cagtccgaca atggataacg atgtaactag taaaccagaa gtagaatcaa
    15121 ctaataacgg cacaactgat aaacctgtaa cagaaactga taatgcaacg ccagcagaaa
    15181 gcacaacaaa taacaatagt acaactacag caacaaacga gaatgcacca acaggatcaa
    15241 cagcaacggc tccaacaact gcgagcacag aagcagcatc atctgctgat agcaaagata
    15301 atgcatctgt aaatgattca aaacaaaatg ctgaagtaaa taatagtgct gaatctcaat
    15361 caactaatga caaggttgca caaccaaaat ctgaaaataa agctaaggca gaaaaagatg
    15421 gtagtgattc aacaaatcaa agtatggttg aatcaacaac tgaaacattg ccttcagcag
    15481 acataacaga accaaatgta ccttctaata catcaaaaga taaagaagaa agtacgacaa
    15541 atcaaactga tgcaggacaa cttaagtctg aaactaacgt tgcatctaac gaagcagata
    15601 aatcgccaag caaagctgat actgaagttt cgaataaacc atcaacatct gcatcttctg
    15661 aagcaaaaga aaaaatgact tcaactaatg ttagccaaaa agatgatacg gcaacagcag
    15721 atactaatga tacgcaaaaa tcagttggtt cagctgcaaa caataaagct acgcaaaacg
    15781 acggtgccaa tgcatctcca gctacagttt caaatggaag caatagtgct aatcaagata
    15841 tgcttaatgt aactaacact gacgaccacc aagctaagac aaaatcagct caacaaggaa
    15901 aagttaataa agctaaacaa caagctaaaa ctttaccaga tactggtatg tcacataatg
    15961 acgatttacc atatgctgaa ttagctctag gtgcaggtat ggcattctta attagaagat
    16021 tcactaagaa agaccaacaa actgaagaat aaaatttatt caagaatgtg aacatctagt
    16081 tatttcatag ggaaatattc cttactaagt aaccaagtct aacagttaat atgaatttgt
    16141 tttagtaatg accaatagcc attgaatagg tggctgtggc tcgaaaataa aacaaattat
    16201 taacgcaaaa atactccttt catacataac acaagttaag tgtctatgtt gaagggagta
    16261 ttttttatgt tcagaaattt aatattgtta attcaaggga actactacat tattactgat
    16321 tgttaacgaa taacgttgga aacttcttgt ttcaaacaaa gctcaataaa ttatgaaagt
    16381 gaattgtacg cttcaattat ttcatcggct aagccatcgt caactttagc atattcactt
    16441 aaaaacgctt ttaaaccgtg ttctttaatg taattatttt tctcaacagt ttcttggtct
    16501 gctgtatcat catacttcaa taacaatgca gctgtattta ataaaccagt gcgttctaaa
    16561 tctttattat aaagatatgt taatggtttt ataattctat ctttagggcc aattttacgt
    16621 aatgtacctc gtccgacacg tgttacttca tcagataaat aagaattgtt gaaacgatct
    16681 attatttttt caacataagc agcttgttcc gcttcagtaa aatcaaattc attagtaata
    16741 tattgactag tttcagctaa aacacggcgt aagccagctt caattgaact atcttcaact
    16801 gcatctaaaa ctgtagcttt acctgcaaat ttaccagcat acgctaaata tgcatgtcct
    16861 gtattcacag ttaataattt acgctcaata tatggtgtta aatcatcaac atatttaata
    16921 tggtttagtt ctggaccata ccatgcatct ttttcaacaa cccattcgta aaatggctca
    16981 accataacgt ctaatatatt ttcattcttt tgtaatggta caattctatc aactgctgag
    17041 ttagcaaaat gaatattgtt accaagaggg ccagtaatat caagtactgc ttttttcaat
    17101 gtatcagttg ccataatagc attctcacaa gcaacaatat taacatggtt tgttttttct
    17161 tttaagaaag gcgcaaaaga tttagcaata ataggtagta tgttaacacc aacagctgtt
    17221 gtaataatat cagcttctag aattgcttgt ttcaacgctt cagaaggttg cattgaatta
    17281 attgcatcaa cattattcac gcgcgtcgtt gttttagact catcagctaa aataacatcg
    17341 tattgatgat catgagctaa agcattaatg atttcttcat tgacgtctgc aaatgttact
    17401 ttaacattgt tgtctgcaag aatataacca atgaaaccac gacctatgtt accagcacca
    17461 aagtgaactg ctttcattat gcatctgcct cctcgaatac ttgtttaatt tcttctgcag
    17521 attttgcttg aacgatacga tccacatttt cttcttcgct aaatgtaatt gcaattttag
    17581 ataacaagtc taaatgttcg ccatctttac cagcaattcc cacgacaact ttaactactt
    17641 cgccatccca gtcaacgcct tcagggattt gtaataatgt taaacctgag tgaatcacat
    17701 ttgttttagc ttcatctgtg ccatgaggaa ttgctaagcc atttcccata aatgttgata
    17761 cgacttgctc acgatctttc attgcttgaa tataagcatc tgttacagca ccactatcaa
    17821 ctaaggcttt acctgctttt tcaattgctt cattttggct gttaacattt acatttaaaa
    17881 agatattgtc attactaaat aattcgctca ttttgtacac tcctaaataa attgtttttt
    17941 aattcgttct aaaaatttgt ttcttaataa tgtttcaagt tcctgtggat tttccataaa
    18001 agtatcaata gattctaatt tcaaacttaa aaattcggac aagtcactta ccagtgatgc
    18061 cataaaatca ttgtcagaaa taaacatgga aatcatatat ttaattgttt gattgtcatt
    18121 ttgaatacta ggcaatgtta acggttcttc taaaactgtg attagaatca tagggtgttt
    18181 aatcatattg tctcttaggt gtggtattgc aacaggatat ggacttaata tccatcctgg
    18241 attgtcaatc aatcgttgct ctagtagttc agcgaatgac gttggatcgt ttattacatc
    18301 gcattgatat agataatctg cgatatatgt ttgccactgt ttaacttcgg tactaactat
    18361 tttcactgaa tcaagcaaag ttaaacccga acgcatatga tgcaataatt gttctgattt
    18421 ttcgtcactt aaatgttttt cgaaaactga agttaagtat gaagtttgtt cattttcaaa
    18481 agatgtgttg ccatcaacat ctttcgtttc aacatgaaca tcatccttat caatcatgtt
    18541 tgatgattta tcatgcgtct cttggaacgt agactttgta tttaaaaact gtgcgacata
    18601 actgatatca ctatctggta ataatgggtt taccgttaaa tagggggagt cgatgtctaa
    18661 attcacagta gaaataatgc catcatattg acttaaatct agtgacttca aatcgctgac
    18721 tgatgcttgt gtaatacgct caatctcact aaaaacttga tctagacgag ttgataatag
    18781 acgactagtt cccataccac tgctgcaaac gactaatatg tttaaaaatc gattaccttg
    18841 gtttttaatc gagccaccaa agtgtaaaac tataaaagca atttcactct ctggaaaaat
    18901 caaatcaggc caacaatcat ttaaagctaa tcttacattt tcaaatagtc ttggatattt
    18961 gaacttaatc atgtctgtta acggattata ggtctcgata ttagcttgca aacgattgat
    19021 tgcaggatta aggtgtagcg tcagtccatc tactaaagta tccaaatctg caaatgtcat
    19081 accggaaatc atttctactt tgttaacaaa cgctttaatt ttgttttctt cacaacgatt
    19141 atttaatgat gtatcattaa gattttttcg tttagctcca cgtaaatgga tagtaatgaa
    19201 agcaatttct gcctgattaa acgtgacgtc atatatttga ccaagtttat cagcaagttc
    19261 gcttgctact ttgtgttcaa atgtgttttg tacagaatca taaatatcat cgtttaatgc
    19321 gacatactct ccatttttta tacgtgaaat ggagagcacg atatggacag ttaaagttaa
    19381 ataacttgat tcggtaagtt ggtagggtaa ggcacttagg tagtccatta atagtctttc
    19441 aacattaaaa atgcgctcta agtcaacaaa gtctttgtgg atttgattta atgaatgaaa
    19501 gacaaaatga ttttcaatta ctgaataaac gctagtacta tttaagttat tcaccattaa
    19561 ttgacttaaa aattcacgtt tctttgattc agtacctacc aagtaaatgc cttcgccacg
    19621 ctttcgagat aaagatagtt ggtacttatt taaatcaagc tctaaatcat ctaacatctt
    19681 tgctaaagtc tggacagaaa cgccaatttc ttgcgctaaa ctatattgtt taactggctc
    19741 cttggcttgt atcaaagcgt atataataat tactttctgc tcttcaacag ataagtcaat
    19801 ggtttgatgt tgtgcaatcg attgcttcaa atcgtttaaa tcagaatctg tgccagcaat
    19861 gcgtagccct tttttgtttg cgcgttctaa agttaatgaa aatgaagtta aatatgcttc
    19921 aacacctttt aattcacggt gaatagtacg agaggacacc gcaagttgct gagcaatgtc
    19981 gtgtatagtg atatattgac cgtggtactt aatcaatagg gctatcattt ctttttcacg
    20041 tgtactcaat aacatggcgg tatcctccat tttttaattt atttaattat tatgcttgat
    20101 catctttttt tagattattt aaaagttctt catatcttgg tgaattaagg aaattatcca
    20161 ctgaaatatg gatggcattt ggtgtttgtt taatagcacg atcagttagt tttttctgag
    20221 tgataactaa ttgagcatct tttggcaatt gattaatcgc agtatttgta actgtaatat
    20281 cattaatgcc cgccttttta aatttattac gtaacatgct tgcacccatt gcactagaac
    20341 ccatacccgc atcgcaggca aaaataacat gatttatatt attgtaattg tgtgcatcaa
    20401 catcttcagt gttgtaatta tccaatagcg cttcaggatc atcatctgat gaagatgttt
    20461 cactaacatt accactagca ttttcttctg tattaacatt tttatcagaa gatactaact
    20521 tagaagcaac gcttgatttt ttccctttag tattttccat ttgagctgtc gcagcttcta
    20581 aatcctgctt tggttctcta gtgaacttca taattaaagc agctacaacg aatgatacaa
    20641 gtgcagcaag gaagacaccg agtaacatgt gcaagaattc acctctaggt gcatttaaac
    20701 agtaaactat aaatgaacct ggtgacgcgg gacttttaaa tccaaatcct gttgcttgat
    20761 aagttgcaac accagtcatt ccacctaaaa taacagcgat aaataataaa ggacgcatta
    20821 atacatatgg gaaataaatt tcatgaatac cacctaagaa gtggataatt ccagcaccat
    20881 atgacgttgc ttttgcagtg ccttttccaa aaatcatata agcaagtaag atacctaaac
    20941 ctggtccagg gttagattca attgtgtata aaattgattg accagctttt gcagcttgat
    21001 ctgcaccaag cggtgtgaat acaccatggt taatcgcatt gtttaaaaat acaatttttg
    21061 caggctctac taaaatactt acaagtggaa gtaggtgtgc atgtactaat gcttcaactg
    21121 ccactgataa aatatgcata ataaatttca taagtggtgc taaaatttta aatcctgcaa
    21181 tcgtcatgat aaatcctaaa ataccagcag aaaagttatt aaataacatt tcaaaacctt
    21241 gcggcgttct aggttgaatc aattggtcgg tcttcttcat taaccaacca acaagtggac
    21301 ccataatcat tgcaccaagt aacattggtg tatcaggtaa tgcaacgatg acccccatag
    21361 ttgctgttgc tgcgatgata ccaccacgta aatcataaat taaacgacca ccactaaatg
    21421 cgatcaataa tgggattaaa taagtaatca ttggtcctgc taaagtagct aaatctttgt
    21481 taggtaacca tccattatct ataaaaatgg ccgcgataaa accccaagcg atgaaagcgc
    21541 caatgtttgg catgatcata ctacttaaga atgatccaaa tgcttgaaca cgacgaccaa
    21601 ttcctttttt ctcttcagtt tgtgacatgt ttacacctct tttttaatat atgtgactat
    21661 atatagtgta tagagaggtt acttgttact caatataaac aaaaatcaac tttgtcaaaa
    21721 taaatgtgac aaaattaaat aaagtgtcat caatgtgaca gtatagatat tttgaaaaag
    21781 taaaacaaaa aaattgtttt aggattttta aaattttatt gtgaaaatat ttgcaaaaca
    21841 aaacaacacc gttgtacaat aatgattaat ggaaaggggg aaagttcggc agtacagtta
    21901 aagcgcctgt gcaaataaat atttgtattt gaagattaaa ggttaatata tgagtggcct
    21961 ttatagagtg caatatatgt atttgtagac gaggaggata gtgatcgaat agatcggcgg
    22021 atgctatccc ggatgtggct cattcgttag cttattaagt aaaacattag ggtgacttaa
    22081 tggacaaagt taataagatc gccagaaatt gaatataaaa aatattaata tggaaagtac
    22141 agtgtgagca atttgtatag ttgtaaaaat aactatgctt aatttgttat ggatgaatgc
    22201 gatgatagca tgttcctatt tatattatga aagcagattg tcaatctaaa ttatcggcaa
    22261 taaatcataa tttacgcgta ctattccaat atggaggaaa atgtcgttat gtgtggaatt
    22321 gttggttata ttggctatga taatgccaaa gaattattat taaaaggttt agaaaaatta
    22381 gaatacagag gttatgactc tgcaggtatc gcagtagtaa atgatgataa tacaactgta
    22441 tttaaagaaa aaggtcgtat tgcagaatta cgtaaagttg ctgatagtag cgattttgat
    22501 ggacctgttg gaatcggtca cacacgttgg gcaacacacg gtgtaccgaa tcatgaaaac
    22561 tctcatccac atcaatcatc aaatggccgt tttactctag ttcataacgg tgttattgaa
    22621 aactatgaag agttaaaagg tgaatactta caaggtgtat cattcatttc agaaacagat
    22681 acagaagtta tcgttcaatt agttgaatac ttttcaaatc aaggactttc aactgaagaa
    22741 gcatttacaa aagttgtgtc attattacat ggttcatatg cattaggttt attagatgct
    22801 gaagacaaag acacaatcta tgttgctaaa aataaatcac cattattatt aggtgttggt
    22861 gaaggtttca atgttatcgc atcagacgca cttgcaatgt tacaagtgac aagcgaatat
    22921 aaagaaatcc atgaccatga aatcgttatt gttaaaaaag atgaagttat tattaaagat
    22981 gcagatggaa acgttgtaga acgtgattca tatattgctg aaattgatgc atcagatgct
    23041 gaaaaaggtg tttatgcaca ctacatgtta aaagaaattc atgaacaacc agcagtaatg
    23101 cgtcgtatta ttcaagaata tcaagatgca gaaggtaact tgaaaattga tcaagacatc
    23161 atcaatgatg ttaaagaagc agaccgcatt tacgttattg cagcaggtac aagctaccat
    23221 gcaggtttag taggtaaaga atttttagaa aaatgggctg gcgtaccaac tgaagtacac
    23281 gttgcatcag agtttgtcta caacatgcca ttattatctg aaaaaccatt gttcgtttat
    23341 atttctcaat caggtgaaac tgcagatagc cgcgccgtat tagttgaaac taataaatta
    23401 ggtcataaat cattaacaat cactaatgtt gcaggttcaa ctttatcacg tgaagcagac
    23461 cacacattgt tattacacgc gggtcctgaa atcgcagttg catctacaaa agcatatact
    23521 gcacaaattg cagtattatc aatcttgtct caaatcgttg caaaagagca tggtcgtgaa
    23581 gcagatattg atttattgag agaattagca aaagtaacaa cagcaataga agcaattgtt
    23641 gacgatgcac caattatgga acaaattgct acagatttct tagaaacaac acgcaatgca
    23701 ttctttatcg gacgtactat tgactataac gtaagtttag aaggtgcgtt aaaacttaaa
    23761 gaaatttctt acattcaagc agaaggtttt gctggtggag aacttaaaca tggtacaatt
    23821 gccttaatcg aagaaggtac accagttgta ggtttagcaa cacaagagaa agttaattta
    23881 tcaattcgtg gtaacgttaa agaggtagta gcacgtggtg cacatccatg tattatttct
    23941 atggagggtc ttgaaaaaga aggcgacact tatgtcattc ctcatgtaca tgaattgtta
    24001 acgccattag tatcagtggt tgcattacaa ttaatttcat actatgcagc attacacaga
    24061 gatttagatg ttgataaacc acgtaacctt gctaaatcag ttactgtgga ataattcact
    24121 tttttagaat caatcatgta ttaaaattaa agtatatggc acccttttag attaatcgac
    24181 tagaagggtg cttttttagg tcgacttagc ttttacttca tcttaatttg gcagaaatgc
    24241 gtaaaaatga agtgttttat ttatttaaat agtctgacaa ttaagggtgt tatgttaata
    24301 tgattttatg agaagtatgg agtagcaata aaggggtgac ctcgcatgtt aattcaatta
    24361 gatcaaattg ggcgaatgaa gcaaggaaaa acaattttaa aaaagatttc ttggcaaatt
    24421 gctaaaggtg ataaatggat attatatggg ttgaatggtg ctggcaagac aacacttcta
    24481 aatattttaa atgcgtatga gcctgcaaca tctggaactg ttaacctttt cggtaaaatg
    24541 ccaggcaagg tagggtattc tgcagagact gtacgacaac atataggttt tgtatctcat
    24601 agtttactgg aaaagtttca agagggtgaa agagtaatcg atgtggtgat aagcggtgcc
    24661 tttaaatcaa ttggtgttta tcaagatatt gatgatgaga tacgtaatga agcacatcaa
    24721 ttacttaaat tagttggaat gtttgctaaa gcgcaacaat atattggtta tttatctacc
    24781 ggtgaaaaac aacgagtgat gattgcacga gctttaatgg ggcaacccca ggttttaatt
    24841 ttagatgagc cagcagctgg tttagacttt attgcacgag aatcgttgtt aagtatactt
    24901 gactcattgt cagattcata tccaacgctt gcgatgattt atgtgacgca ctttattgaa
    24961 gaaataactg ctaacttttc caaaatttta ctgctaaaag atggccaaag tattcaacaa
    25021 ggcgctgtag aagacatatt aacttctgaa aacatgtcac gatttttcca gaaaaatgta
    25081 gcagttcaaa gatggaataa tcgattttct atggcaatgt tagagtaaat attttgcaaa
    25141 taataagtaa taatgacaaa atttaattaa gataaaatgg acagtggagg gcaatatgga
    25201 taacgtaaaa gcaatatttt tggacatgga tggaacaatt ttacatgaaa ataatcaagc
    25261 atcaacgtat acgaaagatg tcattaatca attgagagag aaaggatata aagtattttt
    25321 ggcaacagga cgttcgcatt ctgaaataca tcaacttgta cctcaagatt ttgcggttaa
    25381 tggcatcatt agttcaaatg gaacaattgg agaagtagat ggagaaatta tcttcaagca
    25441 tggtttatca ttggctcaag tgcaacaaat tactaattta gctaagcgcc aacaaattta
    25501 ttatgaggta tttccttttg aaggtaatag agtttcttta aaagaagatg aaacatggat
    25561 gcgagatatg attcgtagtc aagatcctat taatggcgta agtcatagtg aatggtcttc
    25621 aagacaagat gcgcttgctg gtaagataga ttgggtaact aagtttcctg aaggtgaata
    25681 ttcaaaaatt tatctattca gttctaattt agaaaaaata acagcattta gagatgaatt
    25741 aaagcaaaat catgtgcaac tacagattag tgtttcaaat tcatcaagat ttaatgcgga
    25801 aacaatggct tatcaaactg ataaaggtac aggcattaaa gaaatgattg cacattttgg
    25861 tattcatcaa gaagaaacgt tagttattgg agatagcgac aatgatagag caatgtttga
    25921 atttggtcat tatacagttg ctatgaaaaa tgcacgccct gaaatccaag cattaacttc
    25981 agatgtaacg gcatacacga atgaagagga tggcgcagca aaatatttag cagagcattt
    26041 tttagctgaa taataaaata ggtagttatt tattatttaa tttacaatag ttgatgagta
    26101 atgtacaaag agcagtaaag ttattttcta ttagaaaatg tcttactgct cttttgtatg
    26161 cttataaata tttgaatcat ctatatttaa ttggacaaac tctatgagaa taaatattgt
    26221 taaaactaat aagataggaa attcattgat tttgaataat atttcttgtt ttaaggttta
    26281 actattgaat tgtatacttc tttttttagt agcaacagat ccgtctttaa aaaaattcac
    26341 acctattgac tgtttttgtt cttgattata ttctctatga agtgttaatc cactataaaa
    26401 tcaaccggtt cgtttacata tttcccca
//
LOCUS       DAKTIP010000015        25384 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAKTIP010000015 DAKTIP010000000
VERSION     DAKTIP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 25384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.015"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            19..888
                     /locus_tag="O0305_000507"
     CDS             19..888
                     /locus_tag="O0305_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0273665.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVF
                     IKSFKKYIANEQL"
     gene            complement(947..3556)
                     /gene="clpB"
                     /locus_tag="O0305_000508"
     CDS             complement(947..3556)
                     /gene="clpB"
                     /locus_tag="O0305_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCY0273666.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(3759..5573)
                     /locus_tag="O0305_000509"
     CDS             complement(3759..5573)
                     /locus_tag="O0305_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0273667.1"
                     /translation="MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            complement(6120..6428)
                     /locus_tag="O0305_000510"
     CDS             complement(6120..6428)
                     /locus_tag="O0305_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCY0273668.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            6542..7366
                     /locus_tag="O0305_000511"
     CDS             6542..7366
                     /locus_tag="O0305_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0273669.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            7418..8734
                     /locus_tag="O0305_000512"
     CDS             7418..8734
                     /locus_tag="O0305_000512"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCY0273670.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEINAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(8905..9294)
                     /locus_tag="O0305_000513"
     CDS             complement(8905..9294)
                     /locus_tag="O0305_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCY0273671.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGRSPFFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(9623..10525)
                     /locus_tag="O0305_000514"
     CDS             complement(9623..10525)
                     /locus_tag="O0305_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCY0273672.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
     gene            complement(10691..14344)
                     /gene="addA"
                     /locus_tag="O0305_000515"
     CDS             complement(10691..14344)
                     /gene="addA"
                     /locus_tag="O0305_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCY0273673.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPKNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            complement(14345..17821)
                     /gene="addB"
                     /locus_tag="O0305_000516"
     CDS             complement(14345..17821)
                     /gene="addB"
                     /locus_tag="O0305_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCY0273674.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFNQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            complement(17981..18556)
                     /gene="lepB"
                     /locus_tag="O0305_000517"
     CDS             complement(17981..18556)
                     /gene="lepB"
                     /locus_tag="O0305_000517"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0273675.1"
                     /translation="MKKEILEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIVGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            complement(18572..19096)
                     /gene="lepB"
                     /locus_tag="O0305_000518"
     CDS             complement(18572..19096)
                     /gene="lepB"
                     /locus_tag="O0305_000518"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCY0273676.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDQDNNKHDSRQFGLIDKKDIIGNVSL
                     RYYPFSKWTVQFKS"
     gene            complement(19101..19676)
                     /locus_tag="O0305_000519"
     CDS             complement(19101..19676)
                     /locus_tag="O0305_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCY0273677.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            complement(20001..21332)
                     /locus_tag="O0305_000520"
     CDS             complement(20001..21332)
                     /locus_tag="O0305_000520"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCY0273678.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            21683..22888
                     /locus_tag="O0305_000521"
     CDS             21683..22888
                     /locus_tag="O0305_000521"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCY0273679.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            22878..24257
                     /gene="argH"
                     /locus_tag="O0305_000522"
     CDS             22878..24257
                     /gene="argH"
                     /locus_tag="O0305_000522"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCY0273680.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPSYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            24499..>25384
                     /locus_tag="O0305_000523"
     CDS             24499..>25384
                     /locus_tag="O0305_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0273681.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDG"
BASE COUNT         8391 a         4480 c         3678 g         8835 t
ORIGIN      
        1 tttgaaagag gtaagttcat gaatttagat tggtactata catttgtcat tttagcgaag
       61 acgttaaact atcgattagc tagtgaagaa attaatctca ctataccttc tatacataag
      121 caaattaaaa atttagaaca gcatctcaat gtgaaactat ttgaaactta taaaaatcaa
      181 attatcttga ctgaagatgg tcatacattt cttccaattg cgcaaagttt tattgaacaa
      241 tatgaaagtg gcatcaaaca tatccaactt aaaaaaacga tgtttcaatc gaaattaaat
      301 gttgtcgtgt cttcatatat cgcgacattc attatgccca aatttttaaa atctttcttt
      361 aacgagcatc cttttatcga tgtttctctt catgtcaaaa atgaaaatat tgaaaaagat
      421 attaataatc atacgtatga catcgggatt agcagaaatc aacctaaact aagagaagtt
      481 cattctgaaa aagtatgcga aggtaaaatt gtattgattg ctcccaataa agagaataat
      541 catcttctaa ctgaggcatc tttatttgaa aaatacaaaa taataagtga taaccatcca
      601 gaatattggt catcacttaa aaataatatt ttaaatattt atgaaaaagc acaatttcta
      661 tctatcaatg atgtgcatac ttcaattaaa ttaatcgaaa tgaatcaagg tatatcattc
      721 ctgcctcttt atataacaac aaatagtgat tacaatattt cggttattaa cacgaaaatt
      781 ttacaagcac ctatttcatt tacatatata tacagcaaaa aagaaagccc agaaatattg
      841 gtgtttatta aatcatttaa aaagtatatt gccaatgaac aattataata aatttcaaat
      901 ctaaaaaacc aagaatgcga ttaatcatca cattcttggt tcaattttat tcatgaattt
      961 tttcaacatt aaacgttaag ttattgtctg aatttaaatt aactttaatc gttgtacctt
     1021 ctgggaatcc ctctttaatc atcatacgtg ctaatggtgt ttcaatttgg cgttgtacaa
     1081 aacgttttaa tggtcttgca ccgtattgag gttcataagc ttcttgacct agccaagctt
     1141 tagcatcatc agaaacttca attgagattc gttgttctaa taatcttata tttaattgcg
     1201 ttaagatttt atctacaatc atactcatgt catcaataga taatggttta aataatacga
     1261 tatcatccat acgattcaaa atttctggtt tgaaatatgc atttaaactt gtcataacag
     1321 ctttttctgt tgattctgta atttcaccag tctcttttac gttttctaat aaaacttgag
     1381 atccaatatt acttgtcata ataataatag tatttttaaa atcaacgcta cgtcctttag
     1441 aatcagttaa acggccttca tctaaaattt gcaataatac attaaagacg tcagtatgcg
     1501 ctttttcaac ctcatctaat aaaataactg agtatggatt acgacgaacc gcttcagtta
     1561 attgaccccc ttcatcatgt ccaatatatc ctggaggtgc ccctatcaat cttgatactg
     1621 catgtttttc catatattca ctcatatcaa tacgaatcat atgtttttca gaatcaaata
     1681 atgatgcagc taatgattta gctaattcag ttttacctac tccagttgga cctaggaata
     1741 agaaactacc aataggtctg tttggatctt taatacctgc tcttgctcta actactgcat
     1801 ctgaaaccag gtcaaccgct ttatcttgac ctacaacacg tttatgcaag atgtcactta
     1861 agtgaagtaa tttttcacgt tctgtttcaa ctaattttga aactggtatg cctgtccatt
     1921 ggctgacaat atcgccaatt tcttcgtctg ttacaacttc acgaatcatt cgatctgtat
     1981 cttcaccttg ctcatcttgg aaattatcct ctaattctct aagttctttt tccaattgag
     2041 gaattgttcc atattgtagt tcagcagctt tttctaaatt gttatttgtt tgtgcatctt
     2101 ccaacgcttg tctactttca tctagttgcg cacgtttttc ttgtaaattt gctatttttt
     2161 ctttttctga ttctacacga gattgaagtg ctgcttgttt ctctttttca ttggcaagct
     2221 cttcttgtag ttcttgtaat ctctgtttgc tcgcattgtc agattcattt ttaagtgcgc
     2281 tttcttcaat ttctaattgc atgacacgtc tattaacttg atccaattca gttggatttg
     2341 atcccatttc cgtacgaatt gttgcacatg cttggtcaac taaatcaatc gctttatctg
     2401 gtaaaaaacg atcagtgatg taacgatcag acaattcagc ggcagcaact aaggctctat
     2461 cttgaatacg cacaccatga tacacttcat atcgttcttt taaaccacgt aaaattgaaa
     2521 ttgtatcttc aacatcaggc tcactaactg ctactttttg gaaacgacgc tctaatgccg
     2581 aatctttttc aatatattct cgatattcat ttaaagttgt tgcaccaata caatgtaact
     2641 ctcctcgtgc taacattggt tttagcatgt tgcctgcatc catggcacca tctgttttac
     2701 cagcacctac aagcatatgg atttcatcaa taaataatat aattctacca tcagactctt
     2761 taacttcttt taggactgct tttaatctct cttcaaattc accacgatat ttagcgcccg
     2821 ctactaatgc gcttaaatct aactcaaaaa cagttttatc taataatgat tctggcacat
     2881 ctttcttaac tatacgttgc gctaatcctt caacaattgc agttttacca acacctggtt
     2941 caccaatgag cacagggttg tttttagttt tacgacttaa aatacgaatc gtatttcgaa
     3001 tttcttcatc tcttcctata acaggatcca ttttaccttg tctaacttct tctactaagt
     3061 cgcggccata tttagctaat gcttcgtaat taacttctgg attttgtgat gtcacgtgat
     3121 ttccccctct tactttttta ataatttctt tgataacttc tactttatta tttatataat
     3181 gttttgttgt ttgatcaatg tccattgccg aacgtaaaat atgctccatt gaaatatatt
     3241 catcttcata ttctttcatg tatgattcag ccttagttat caattggttt gcttgttggc
     3301 taatatattg accatattgt atattgtcac cttctacaga tgcatacgtg tttagtttgt
     3361 cttcataagc tttgtttaat tgatctacct caatatttgc tcgttctaaa atacttttat
     3421 ataagctttc actttcattt aaggcagcgc ttaaaattgc ctcaatttct atattttgta
     3481 atttatgttg ctgactcagt tcaactgctt gttgtaaagc actttgaaca gcatatgtca
     3541 ttttatttat atccatataa tttcacctct aatttgtaga gttggtcaag ttgcactttt
     3601 gactttgact ttctttgacc ttaattatat tatagtccca atataatcat ttatcaactc
     3661 ttttacactt aaatttccta atatttgtaa atattttttt gaataaaaaa atctagctaa
     3721 tgttacgtta cacattaact agacttatta aacatatgtt atttcttttt aagtgctttt
     3781 aatatttcat cttttaaagc taggactcct ttgtactcta aatgtacacc gtctggtgca
     3841 aaatattcac tatgtccttg tgatcgctta taccaatcaa ttaatgtgac attggacttt
     3901 cgtttcgccg cgtcagctaa taatcgattt acatttgcct cataaattct tggaacacgt
     3961 gtattaacta aataaatctt ggcttttcca aattgattaa gtaaatcgtc gagctgtttg
     4021 acagtaaagt cgccatttgt acctaattct aatacgactt gatcagatga ttttttatat
     4081 tgtgaataat tcgctttaac taaaggtaag gtttgataca attgacgccc tacttttcca
     4141 tcaattctag acttaggaac tgatgactta aatgactcac cgatatcaac cattactgaa
     4201 tcaccgatta aaagaggttt aatattatta tatacgtcgg aagattcctt tttcttctca
     4261 cttactaagc catcaatgtg tatgttatca attggtgcta tccgcattaa ataattatct
     4321 tcaattgtgg tatcaaagct attcgctttt tctccaataa tgtctttacc atatttattg
     4381 aatgcaccta ctaaaatcaa cataaatgga attaacaagg ttactacaat tgccattctt
     4441 ataaattgtg gtatataaga aggtcgccaa tttaaagctt taataccttc ttttctaaat
     4501 ggagtttcta taaagcgata tgatagctct gcaaatataa ttgttaaact tatatctata
     4561 aagtacacat atacaggtat ctgtccgtct acatagtaac tatgtacgaa actaattact
     4621 gcaaaatgcc ataaatataa actataagac cttttcccga taaatactaa cactggattt
     4681 gaaaatatct tcgctatcca tgtagatgga tgaacgacac tagcaataat aaataacgtt
     4741 aatatggata ttaaatagaa accaccatca tatatccaat tcgtctcatc attaataatg
     4801 aaaaataata atataagtac tataaatgat aaactaccta tgctatcaat aacatatttt
     4861 acaactttag gtggatcatt tttcaattta aacggtggcc ataaaaaagc taaaataaca
     4921 cccagtaaca atgtctgtaa tctagtatct gtaccaaaat acactcgtga atgatcccca
     4981 ttaatactat agataaacat cattaaccct aaagaaatta ttgatactcc ccaaaaaata
     5041 aatcctattt tgtatcgctt tttaattgtt aacaataatg taaccaaaat aactgggaaa
     5101 aatatgtaaa actgttcttc aattgctaaa gaccataaat gctttaatgg cataaatgaa
     5161 aattgctcaa aataattaac atcttttgct atataccacc agtttgatac ataaaatatc
     5221 gcagcaataa tatcatgttt aaccctaatg atattatctg attttaataa taaggttgct
     5281 gtccctacaa ccattaataa aactatgact gctggtaata aacgttttaa acgacgtatc
     5341 caaaagcttt tcaatttaat gatacctgtg tcatcatact ctttgagtaa taagcttgta
     5401 attaaataac cagagatcac aaaaaatgta tccacaccta aaaagccacc tgtcaaccat
     5461 tgcttattta agtggtaaat aataattcct agaacagcga ttgccctcaa accatcgagc
     5521 cctggaatat atctcatttt cttatacttt gtaaaaccct ttgttttgtt catttttgca
     5581 ttcttccctt ttaaaactgt tcttcttaga tgcttaatta aatttagtta tgctgtttaa
     5641 aagaatattg aaatgcatat gtatattatt gaattacgac atcatcaaaa tcatattgac
     5701 taaaatactg ttaaattaaa aaaattacca atgatgattc ttacttcgaa atccaatttg
     5761 taatgcaact cggcaactta aaaactatga agtattatgt attgtaatat aactgtaata
     5821 taaattaaat ttattataaa aattttcaag aaaatattca actagaaaat gaattgtaca
     5881 ctcttggaag tgcaagtcac tgtcttaatt catatttttt gaaacaagtt agatataaat
     5941 tttcaaaata aaatcagaaa ctagaacata aataaggctc ccttcaaaat tttcattttt
     6001 caatgtctac tttgaaggga gcttattcac aatgaattat actctacaat gttatattga
     6061 ctgcgggccc aaacacagag aatttcgaaa agaaattcta caggcaatgc aagtttatgt
     6121 tagctcacac caagtgcaat cttagcgtaa cgtgacatca tatcttttgt ccaaggtgga
     6181 ctccatacga tattcacttc agtatcctga atttcaggaa tctctgctaa tactgtttta
     6241 acttgatcaa taatttgagg tcccattgga catcccattg atgttaaagt catatcaact
     6301 gtacatacgc cttcatcatc aacattcact ttgtatacta aacccaaatt aacgatatca
     6361 attcctaatt cagggtcaat taccatttct aatgcaccta agatactatc tttcaatgcc
     6421 tcttccatcc atatcacctc tttaatgtca tattattcat aatatatcaa atatccgaca
     6481 aaacgccaat aaaatgctat gatgtatcta tatgaactaa gcaacttatg aggagagaga
     6541 tatgcaacca catttaatat gtctagactt agacggaaca ttattaaacg ataacaaaga
     6601 aatttcatca tatactaaac aagtattaaa tgaattacaa caacgtggac accaaattat
     6661 gattgcgact ggcagacctt atcgtgcaag tcaaatgtat tatcatgaat taaatttaac
     6721 gacaccaatt gttaatttta atggcgctta cgtacatcac cctaaagata aaaacttcaa
     6781 aacttgccat gaaattttag atttaggcat cgcacaaaac attattcaag gattacaaca
     6841 atatcaagta tcgaatatta tagcagaagt gaaagattat gttttcatta acaatcatga
     6901 tccaagatta tttgaaggtt tttcaatggg taatccaaga attcaaactg gtaatttact
     6961 tgtccacttg aaagaatccc ctacctcaat tttaattgaa gccgaagaaa gtaaaatacc
     7021 tgaaatcaaa aatatgctta ctcattttta tgccgatcat attgagcatc gacgctgggg
     7081 cgcaccattc cctgtcattg aaattgtaaa acttggtatt aataaagcaa gaggcattga
     7141 gcaagttaga caatttttaa atattgaccg aaataatatt attgcattcg gtgatgaaga
     7201 taatgatatt gaaatgattg agtacgcgcg tcacggtgtt gctatggaaa atggtttgca
     7261 agaacttaaa gatgtagcga acaatattac attcaacaat aatgaagatg gcattggtcg
     7321 atatttgaat gatttcttta atttaaatat tagatattac tgttaattta taactaatca
     7381 ttttataata ttttaaaaca ataggaggta agttacgatg cccaaaatag tcgtagtcgg
     7441 agcagtcgct ggcggtgcaa catgtgccag ccaaattcga cgtttagata aagaaagtga
     7501 cattattatt tttgaaaaag atcgtgatat gagctttgct aattgtgcat tgccttatgt
     7561 cattggcgaa gttgttgaag atagaagata tgctttagcg tatacacctg aaaaatttta
     7621 tgatagaaag caaattacag taaaaactta tcatgaagtt attgcaatca atgatgaaag
     7681 acaaactgta tctgtattaa atagaaagac aaacgaacaa tttgaagaat cttacgataa
     7741 actcatttta agccctggtg caagtgcaaa tagccttggc tttgaaagtg atattacatt
     7801 tacacttaga aatttagaag acactgatgc tatcgatcaa ttcatcaaag caaatcaagt
     7861 tgataaagta ttggttgtag gtgcaggtta tgtttcatta gaagttcttg aaaatcttta
     7921 tgaacgtggt ttacacccta ctttaattca tcgatctgat aagataaata aattaatgga
     7981 tgccgacatg aatcaaccta tacttgatga attagataag cgggagattc cataccgttt
     8041 aaatgaggaa attaatgcta tcaatggaaa tgaaattaca tttaaatcag gaaaagttga
     8101 acattacgat atgattattg aaggtgtcgg tactcacccc aattcaaaat ttatcgaaag
     8161 ttcaaatatc aaacttgatc gaaaaggttt cataccggta aacgataaat ttgaaacaaa
     8221 tgttccaaac atttatgcaa taggcgatat tgcaacatca cattatcgac atgtcgatct
     8281 accggctagt gttcctttag cttggggcgc tcaccgtgca gcaagtattg ttgccgaaca
     8341 aattgctgga aatgacacta ttgaattcaa aggcttctta ggcaacaata ttgtgaagtt
     8401 ctttgattat acatttgcga gtgtcggcgt taaaccaaac gaactaaagc aatttgacta
     8461 taaaatggta gaagtcactc aaggtgcaca cgcgaattat tacccaggaa attccccttt
     8521 acacttaaga gtatattatg acacttcaaa ccgtcagatt ttaagagcag ctgcagtagg
     8581 aaaagaaggt gcagataaac gtattgatgt actatcgatg gcaatgatga accagctaac
     8641 tgtagatgag ttaactgagt ttgaagtggc ttatgcacca ccatatagcc accctaaaga
     8701 tttaatcaat atgattggtt acaaagctaa ataaatgaat catgcgattc aatctaatct
     8761 taattgtcta ggttagaatt atgttggact ggtactacta tccagtccat tttttatgtt
     8821 taacattttt agaatcaaaa aagacataag gtcttggact aataattgtc catgccttat
     8881 gtcatatact atatgtctta tcaattagcc aataccgaat aattttgata taggccctaa
     8941 cggtagaatg acacctaata ccattgtgat gataattaat gcaattgtta tccaaaacat
     9001 tgtgtgactt tgttcatgtc tctttctttt agcaatcgac acttccatca atccaactac
     9061 tgcaacacca cacagcattt tcaatgtaag caacatatga tttgccccgc cattcataaa
     9121 tgactgaatt aatatccaaa atcctgaaat taacgtcaac agcataaata agcgtaaaat
     9181 catgtgcaac ggtttgaaaa atggtgatct gccttgattt tttgaaatgt ttaagtatgt
     9241 agcgataaat aaaataatcg ctaataccca acttaatata tgtaaatgta acatactgat
     9301 tccccccact ttaattattt atattattaa attaaagctt cttgggatta atacccactt
     9361 gcttgtaatt taatcatgat ttgattatac acgaatatat attctaccac acttctatat
     9421 ttgagaggaa gaacatgaca ttttattcct tattagaata ttgtgaatct gctgtaaaat
     9481 aatcaactac ttcttaatat caatatttca ctttcatctc aaaatggtaa cattataaat
     9541 aatttatctt taacaccttt ttagaaaagc aagaaaaaac taaccaatct atataaagac
     9601 tggttagctt tttaaatgat aattatttag cgatataagt tgtcagcgtt ccaatattat
     9661 caatagtcac tttaacttca tcacctggtt gtaaaaattt aggtggttgc atacctgcac
     9721 caacgcctgc tggtgtacca gttgcaataa tatctcccgg atgtagtgca acatattttg
     9781 aaatttcttc tattaattca tcaattttaa gaatcatttc gccagtgtta ccatcttgtc
     9841 taatttcatt gttaactttt gtaacaatat ttacattttc aggtaatggt agttcgtctt
     9901 tagtaacgat ataaggaccc attgggcaac cgccagttaa actttttgat aaaaatgctt
     9961 gatcttgttc actttgtgct ttgcgatcag tgatatcgtt aataattgta tagccgtaaa
    10021 cataatctaa agctaatgct tttggaatct tttcaccaga cttaccaata acaataccta
    10081 attcaccttc ataatctaat tgatcagtaa tatctttatg atttggaatt gttgcattat
    10141 ctcctgttaa agatgacgct gcttttgtaa atacatataa tttttccact tcatgattta
    10201 attcgttcgc atgatctttg taatttctac caaaagcaat cacattattc ggaggtgtta
    10261 ctggtggtaa aaattcaatg tcattaaatg aaattttata gtcttcagct ttgccgctat
    10321 cttctgctgc tacaactgct ttacgtactt gttcttgaaa atctaaagta tgattttgtt
    10381 gtaaaccagc taacaatgtt ttaggatgga aatctccttc tgcaaagtca gcaaatactt
    10441 gtgttaaatc ccatacagca tcttcgcgtt ttactttaac gccatatgaa gttttgtcat
    10501 tatacttgaa tgataagaat ttcattcatt ctcaactcct catctttatc ttaattcaca
    10561 ttataacttt tttcgttatc aaataacaaa taaataagta agacaatttt gaaaatgagt
    10621 tgtgttcatt ctgctacaag gactttgcac ttaatcgaaa ttatttttta ttcttttgaa
    10681 aatcaaaata ctatagttgc aatgtaccaa atttgaagaa gtataaataa cctttaactt
    10741 ctttattaag aatcgtttga agcgtatttt gataatattt catctgtatc ttatatttat
    10801 tttttaattg tgtaccaatt tcttcatctg tcatcccacg gcgacgatta aatgcatcgg
    10861 ttttatagtc tacaaaataa tgcacaccat ctttaacaaa gattaagtca atcatacctt
    10921 gaataattga gacgtcttcg tctccttgtg gcaattggtc aactaatgct tggttaacta
    10981 caaacggtaa ttcacgataa acttgctctg cttcagcaat aatcgaatat aactcactat
    11041 tgataaatgt cattatttca tccatacgga tatctttttt cgcatctgct tcgataatat
    11101 gtttatcgat taatccatcg atatactgat gtaactcaac ttcagatatg cgttcttttt
    11161 tgaatggtaa atgttgcatc actgtatgca ttaacgtacc aatttcattc gcttttcgtt
    11221 taccttgttc acttagaaat ttaggtcgtt catacgttga aaaaccgata cgatattgcc
    11281 ttactcgttc gtaacttgtg ccactttctt ctgtttcata ttgtcttttc aattcagaaa
    11341 cagattgttt tgagggcttt ttagtatcat ttacatatgg atatcgataa tcaagttggt
    11401 gtttaatttg tgctttaaca tcttcattac cattttgcat agtttctaat tgattaaccg
    11461 aacgatattc atcattatct aaaatggttt ctgtagacac atcttcaaag tacacaattg
    11521 aaatatttac attcggacga ctactatctt caatttgtgc tatatctttt tcaaatttta
    11581 aatcatctgg aattgacgca gattgatgtt tagataaaat actataaata agatggaacg
    11641 gatttggtga agttaatcgt tcattgacag caatgtgctc accagaaata gacaattgct
    11701 ctagttctag taatgattta tcatttttca ctctaccaat taaataaagt tgttctttcg
    11761 ctcttgttaa tgctacatag actaatcgca tttcttctga cacaagttct ttttcggcaa
    11821 cagctctata tgcaaccgaa gctaaagatg gaaatgccat ttctttatcc acatcaaaat
    11881 aatccattcc gagaccaaat tgctgattta aaataactgg ttgtttcaaa tcacgtttat
    11941 taaaatcttt tgacaatcca gaataaatga caaatggaaa ctctagacct ttactactat
    12001 gaattgtcat cattctaacg acattatcgt ttggaccaac tacattttcc tcaccaaaat
    12061 ctttgcctct ttcaatcaat tcatcgataa aacgaataaa ttgatataaa cctctaaaac
    12121 ttgaattctc aaactcgata gctttattaa ataaaccata aagatttgca cgtcgtccac
    12181 gtccaccaat aagtccacta aagtattgaa taacataatg atcattataa aatttatcaa
    12241 ttaactgata caccggatga tctttactat attgttggta actttgaata tctgataaaa
    12301 acatttttaa tttatcaact aaaattgcat ctgctgcttc gtcattaatg taatttacaa
    12361 tcgattgata gaagtagtca tcatttggac tcaatattct aatttgagct aattcgtctt
    12421 ctttgaactg atatataacg gagcgcatta acccaactaa ataaatatct tgtaatggat
    12481 tatctatcgc tcttaaaaat gataatacta agcggacttc tgtttgttca aagtaacctt
    12541 cacgactatt cacatggaat ggaatatctt catttttaaa ggcttgttgt aaattgcgag
    12601 cttgtccaaa gctgcgttct agaataacga tatccttgta tgtcgcactt ctatagcttc
    12661 ctgttttcat atcataaact ttttgatgtt ctaagatatc tttaacttgt tctactataa
    12721 aatgcgcttc ttgttcacta ccagttaaat cactatgttc ttgatccgct tcaacaagga
    12781 cttttaagtt tactggatgg tccgattcat catatggtgc accataatac aactgtgccg
    12841 cttcatcata ttttacttca ccgacttgtt catccatcat atgtttgaat atatagttag
    12901 tcgttgacag tacttctttt cgagaacgga agttttgcga caaatcaatt cgacgtccag
    12961 tgccatctcc atctatagta aagcgttgat acttttcaat aaataaactt ggatcagctt
    13021 gtctaaattt ataaatggat tgcttaacat ctccaaccat aaataaatta ccattatgtt
    13081 catcacccgt tttgatgcaa gatagtattt tctcttgaac tcggttcgta tcttgatact
    13141 catcgaccaa tatttcttgg aagtgttgac ggtatgattc ggcaatttct gaaggcgaac
    13201 catcctcatt agttaaaatt tgtaatgcaa aatgttcata atcagaaaaa tccaaaatat
    13261 ttttgctacg cttttttcga ttgaattctg acataacatc tttcacaata cgcgcaaggt
    13321 actttactcg tggcgccaat tgttgcatat cagctttcaa atcatcagct tctcttgaaa
    13381 agtaatcact cttcacttta tcaattaatg atttatattt tttatactga agtttagcat
    13441 cttctaaggc atcgaccatc atttcatttg cttctttaat tttcgctgtt acattaggca
    13501 aacgcgcgcc aaattcgtga ccagttaaat aaggtatatc aataaagcca ccttctaaaa
    13561 cacgccccat taaacgtcgt tcatcttcta taacagctaa atgtttatcg acgctatcca
    13621 tcatactaaa caaatcatac gccttattta aagcatcata agcagctgtg ataaatactt
    13681 tagataagtc tgttagtagt tgaataagtt gcgcttgttg tgcttcttct tcgtatggtg
    13741 tcaccaattg atccaaccaa tttttaggat ttggatttgc aacgctaaag aaatacaatt
    13801 gtttaataat cattcgaaac tgatcatcac ttctatctga agacaattgc tctgttaatt
    13861 caataaaagc aggatcaagg atatcgtaat gttgttctat gacctcatct atcgtttgtt
    13921 ctaataataa aatattttca gcttcactgc ttgttctaaa gttcgggtca atatttaata
    13981 catcataatg ctgttgaatt aatttcaagc aaaaactatg gagtgtagat atttgtgctt
    14041 gatgaatttt gatgcgttgg tttttcaagt gtgcatttgc aggatcagca atcgatgcct
    14101 cttgaatacg ttggtctaca cgatgcttca tttcacgtgc gcttaagttt gtaaacgtta
    14161 cgactaaaag tcgatcgaca tcaatgccat cacgtaaaat cttttggata atacgctcaa
    14221 ctagtacagc tgttttacct gaacctgccg cggctgcaac aagtacatct tgtccagttg
    14281 cgtaaatact ttgccattgc gcgtcagtcc aaatcacgcc ttgtggtttc tctggaattg
    14341 tcatctattg ctcaccccca aattcatcat taatgttaat attttgaatt gcttcaattg
    14401 gatttattgt ttcatctaca gttcgatatc gcttactatc aatcatgcca tctacatgac
    14461 ataccgattg ataactacaa aaagcacatg gcaatttttg tttgtacttt aatggtgcaa
    14521 cttcagtatg tccatccata atatttgaag ctgtttctat aaaattctct ttgttatgtt
    14581 ggatgaattt ataaatcgtt gcttcatctg ccacttggct gcctcgttta ctcaaagagc
    14641 catctttatt caaaccaact ggtacaatat ctgaagtgaa tttaggttct aaacgaatat
    14701 ccaatgcatc aataacagtt tggtctgcat taactaaacc actcaactta aactttttaa
    14761 ttaaatcttg ttctagttta tcttcatcaa tatcagacca tgatttaaat ttaattctag
    14821 gttcatgtac atggaagtat aataatccac ctggtttcac aatatctgtt aatccaaggc
    14881 gttgtttatt ttgtaaaacg atatccatgt atgtcatcat ttgcatttgc ataccataat
    14941 atactttcgt taaatcaagt gtcgcactac cttcagagga tttatagtca atgatattaa
    15001 caaaacttgt atcattcttt gtatacgtat cgatacggtc aatttgccct ctaatattaa
    15061 ttggaatacc ttgagttgtc gttaatgttt gtgcaattaa ttcgtcattt gttcttggtt
    15121 tccttctaaa acttgtctca aaatgttttg gcataaactt tgaatacgtg ccttgatatt
    15181 ttaatgcgct tagtgttgtt tctacaatag cgccaatgcg tcttgataaa taacgatagt
    15241 aagctgaaga atttaataaa ttaaactgaa ctttaggtaa aatttcttcc aatgcttcat
    15301 tcgttaattg tcttattttt ttcaggtcta attgtttaaa atcgccatta atacgttcag
    15361 atatatattt taaaacagaa tggaaaatat cacctaaatc aaagttttga agttcatact
    15421 tcgttcgctc atttagtttc agaccatgtg acgcatagtg tttaaatggg cattgttgat
    15481 aaccttcaaa acgggataca ctggcattga tttccttacc atataaatct ttagacaacg
    15541 tttcacctaa ttttacagtt tcattgtcaa acgttaatgc tgacattaaa taatctaaac
    15601 cttgatttaa atgatcgcta tctctaatta cttgataagc atctaaccaa ctatcagcca
    15661 caatttcatc atataaccaa gcacgcaatg cttcaaataa tgtaattttg gtttgcttag
    15721 catgttgcat tagtgacaat gggttaactt catggtattg aggaatgtta gtaatttcca
    15781 attggttgaa caatgattga atttgattta aaaatgggct gatctcctta tcatcaccac
    15841 ttgatcccat tagactgtaa gaaaatgtaa catctccctt agctctagtc atagcaacat
    15901 aacaaacaaa tgcttcatcc atctgtaaaa tatctgatgt tggacttaac tcgacattag
    15961 cctgctgttc aaagtatttc ttttcttcat ctgtaatcaa gcttgacgca gttactggtt
    16021 gtggcatcgt tccatcattc atacctacta agtaaacatg ttgcttatta tcgactttag
    16081 ccaaatccat cgtaccaata cttacttggt ccaatgtttg cggaatcata acaaattcta
    16141 attgttctaa accaatatca aatacttcta agaaacgttc catcgacatt ggttcatctc
    16201 caaatactag aactaagtca tcaaggattt gaattaagcc attccatatt tgatcaattt
    16261 cctccgcctt ttcatgatta ccatttaaat caagttcatc tcgctctgtc atcaattgat
    16321 ttggcagttc gaaatattcc atactttcat aaaaagcagt tgcaaaatct tttacagttt
    16381 ccgcttgtga catttgcttt tcaaaatgta aaattttatc aatgacatct ttctttaact
    16441 taacgacttg ttcaaatgtg ttacgttcat cttcggtcag tttatgcgct ttacgcccca
    16501 ttttgctaaa atgttcgaca ttaaatagct catcatctaa ccaacgttta ccgtatatac
    16561 cacgttcaag tacaaaattt tcaagtaaat caactaagta tgcacttttt agatatgatg
    16621 ccgttaacac atcagtcttc aataagcgta gcattggatt cacttgccaa ttagattgaa
    16681 taacttcaat caatgaacga atcatttcca tgaccggatg atgtgtcatc gaacgctttg
    16741 tatcaatatt ataaggaata ttataaagcg gtaatatgga atcaaataaa taagcataag
    16801 attcatcacg atataaaata gcaatatctt gatatcgtaa ttgcttatca cgaatatcaa
    16861 cgatgatacg tcgcgcaatt tcatttattt cctctctcat agtcgcagat tctaaaatat
    16921 tgatatgacc ttgacatgcc actcgattga tttgaagtgc atcaaattct tgttcaagat
    16981 gctttaaatc ttgattattg aagcgatata attggttgaa atattgacgt tcaatagaaa
    17041 tattgagttc atttgctatt tcttcaatat gtcgtaacac ttccgatggt tttctaaata
    17101 aactaaattg atcgtggtta ccatctgtcg tcaatataat tgtgacactc ttcgcatatt
    17161 taattaatcc tttgattatt aaatactcaa tcgttgaaaa gttgtgaaaa ccatcaatat
    17221 atatatcagc acgttttagc cactctgatt tcggcataca atcaataaaa tattgtaatg
    17281 aatcctcacc cgtaataaac tcgttttgaa tgcgttgttc gaactcacgg tatattaaag
    17341 caatatcctc taacttattt ttagttcgag tttgcatatt tttatcagca ataaagtgtt
    17401 ctaaatgttc aggcgttact gcatattttt taaaatcttg aatttgttct gttaattttt
    17461 cactaaaccc ataatatttt gcttgtgatt gataaagttt taaatacttt tgttgttctt
    17521 gaacaatgtt ataaatcatc atttccgttg cagctttaga taacttttgt tcgctataac
    17581 taccaacttc ttggaaaata cgatgactta atcgttcaaa atgcaacact tctgttctta
    17641 aactaccatt taattccgga tcattgacaa aggcttgttc taattgaaat gtactttgag
    17701 ttggcgcaat taaaatgatt ggatctccaa gcggatctgc tttcattttt tgttttattt
    17761 cggtcaacat tttcgtagac ttacctgttc ccgctctacc taaataagca tgtaatgtca
    17821 tgacaaaccc tcacttcctt tgacacatat tttaacacaa agtgattgtg attgatttat
    17881 tataagttca tttcgaactt agcttttatt gtggtactaa caaaataaaa catcgtttat
    17941 cagtattaaa accgacaaac gatgttgtat ttgtttcata ttaattttta gtattttcag
    18001 gattgaaatt atgtttaaat tcactaaatg gccagaacct aaatgaaact ttaccaacaa
    18061 tttggtcttc atcaatgagg ccaaacgcac ggctatcttt acttacttca cgattatctc
    18121 caagcactaa atatttacct tttggaatga catttgattt aggattcgca ttcggtaaat
    18181 ctttaacttg gaaagtccca gtaatgtaat caccttgttt atgttttaaa ttgtagttta
    18241 aatatggttc atcttgtttt ttaccattga catataatgt atcatttttg tattctactt
    18301 tatcaccagg aacaccgatg acacgtttaa catagtcatc atttttgttt gcatggaaga
    18361 caactacatt acctttttcc aaaccacctg ttttatatcc aacaatgttt acagctactc
    18421 gctcgccatc tttcaaagtt ggatccattg attcaccttt aattgtatat ggcgtaacaa
    18481 taaatttacc tactataaat aaaatgacaa aagcgactgc aattgaaata atccattcca
    18541 atatttcttt tttcaatttt gacacctctt tttaagattt gaactgaaca gtccattttg
    18601 aaaaaggata gtatcgtaaa ctaacattac caataatatc ctttttatcg attaaaccaa
    18661 attgtcttga atcgtgcttg ttattatctt gatcatttag cacaacaaaa ttgtttggcg
    18721 gaataatatc accatctaat tctttaaaat tgcgcaaact aaaatcttta atttttctgt
    18781 tcttggcata agatgcgtca accggtcggt catcacggta taattgtccc tgacgaaacg
    18841 ccattgattg accaggtttg gcaataattc gactagtata tatctcgtta ccacgcctat
    18901 atgtaatgat atcaccatta ttcaattgat taaatgtaac tttaatttta tttacaataa
    18961 cacgatcccc tttgttaagg gttggtgaca tatcattatt cggaatgaca tgaccaacta
    19021 ttacaaaagt ttgtacgaac agtacaatga taatagcaag tatcaatgaa atcaaatatt
    19081 ttacaacttt tttcacgatg tcactccttt ttcgatccca taaaatgctg ttctaacttt
    19141 tttccaacaa tccaaaatac aaccaacaca actaacatta atatccctct taaaggatgc
    19201 gttaaaattg tagtaatttc cttacctaaa taacctaaaa taattgttga aactaacttt
    19261 gatgatgcca aaacaatgaa ataatattta ggtctaatat gagatagact cgctacaaaa
    19321 tttattaatg tatttggcgt aaaaggaaaa caaagtaaaa taaacaatgg gattaatcct
    19381 tggcgatcaa taaaactaat caagcgttga acagcagtac gttgtttaat tcgctgcatc
    19441 ctctcagtgt tcaccaatcg tttacagatc aaatagactg taaatgttcc agaaattaat
    19501 ccaagccaac taatcaatat acctaaaata ggtccataag cttgaatgtt aattaaaata
    19561 tagagtgcta aaggaaatac tggaattata gctctaatat ataacaatat aaatccaggt
    19621 aaataaccaa actgtcgaaa tatctcaaac cattcttcta cttgatgaaa cgacaaatca
    19681 tcaatccctt tctttggttg aagataatta ttcttacatt ataaagttaa ttactaagta
    19741 aagaaacatt aacatgtaat catgctaata aatcagtctc tagtagtata acaatattat
    19801 tgtaaggcag atattatttt atctttaatt ttctcttaac atcttgtcac attagctagt
    19861 aacctagttc ttgcatataa ataaaaacga ggttgtcatt acatttttac atttgtaatg
    19921 acaacctcga aaatatgctt taaatagtta acgtctagtt ctatcttttc aaacttcact
    19981 aatctttgaa gtaatgtatt ttataaacgt tcttctaatt cttttttcaa gtcttcaaaa
    20041 ccaggtttac ctaataatgc gaacatgttt tgtttatatg cttctacacc aggttggtta
    20101 aatggattta cgcctaattg gtatccactc attgcacaag caagttcgaa gaagtatacg
    20161 acgtaaccga aagtttcttc atctaattgt ggaatgttca ctaccatgtt aggaacacca
    20221 ccatcagtat gcgctaataa tgtaccttcg aatgcttttg tgttaacttc gtcgattgtt
    20281 ttaccagcta aataatttaa tccgtctaga tcatcactat ctttttcaat agtaatatca
    20341 tatttaggat gatttacttt tacaactgtt tcgaataaga agcgacggcc ttcttgtaca
    20401 tattgaccta aagaatgtaa atcagttgtg tagttggcac ttgaaggata gatacctttg
    20461 aagtctttac cttctgattc accaaataat tgtttccacc attcattaaa gtattgcata
    20521 gatggttcat agttaatcaa catttctgtt gtataacctt ttgcatataa aatgtttcga
    20581 attgtcgcat attggtatgc aatgttttct tctaatttat ctgaagataa ttcttcacgt
    20641 gcttttgcag caccaatcat catagcttcg atgttaattc cagctgttgc aattggtaat
    20701 aatcctactg ctgttaaaac agaatatctt ccacctacat catcaggtac gataaacgtt
    20761 tcataacctt cgtttgtagc caactgtttt aaagcacctt tttctttatc cgttgttgca
    20821 aatatacgtt tttgtgcttc ttctttaccg tatctttctt caactaattg tttgaacaat
    20881 ctaaatgcaa ctgctggttc tgtagttgta ccagatttag aaataacgtt tacagagaaa
    20941 tctttgtctg ctaaataatc aactaactct ttcgtatatg ttgatgataa gtgattacca
    21001 acaaatacaa tttcagggta ttcattgctg tttctaaatg atgacgttaa catttcgatt
    21061 gctgcacgtg cacctaagta agaaccacca ataccgatga ctactaaaac atcagaattt
    21121 tctttaatgc gttttgatgc ttcaacaatt cttgaaaatt cttctttgtc gtaatcaact
    21181 ggtaaatcaa cccagcctaa gaagtcacta cctgcaccag taccttcatg aattgttttg
    21241 tgaattgatt taacaatttc ttgttgttgt tttaattcgt gttcaccgaa aaattctaac
    21301 gttttactaa aatctaattg aatatgagtc atctataaaa gcctcctgtt tcatttaata
    21361 tcttaatttt actaagttta cgtttgcgat tcaattcaca agtttgtact taatcaataa
    21421 aattgatatt tatgtgacgt ttcacaccta tttttcaaca aacatttcaa actggcgtca
    21481 aacactcaaa cattttttat acaatttgca tgttttttaa aatactttta aacattgata
    21541 aataagcatt tacacttaca actctaacta tctctatcgc attttatgaa taaatatgta
    21601 aaataatggt tttattttca ttaaaagttt gctatcatgt gttcatacga aatattcaga
    21661 taactacaat gaggtgaatt ttatgaaaga gaaaattgtt ttagcatatt caggaggact
    21721 agatacaagt gtggccgttc aatggcttat cgacaaagga tacgacgttg tagcttgttg
    21781 cctagatgtt ggtgaaggta aagatttaga catcgtttat aaaaaagctt tagatatggg
    21841 agcagttgaa tgtcatatta ttgatgcaac aaaagaattt agtgatgagt atgtaagtta
    21901 tgcaatcaaa ggaaatttaa tgtatgaaaa tgcatatcca ttagtttcag ctttatcaag
    21961 accattaatc gctaaaaaat tagtagagat tgctgagaaa acaaattcag taggtattgc
    22021 acatggttgt actggtaaag ggaatgacca agtacgtttc gaagttgcca ttaaagcatt
    22081 aaacccatca ttgaaagcat tcgcacctgt acgtgagtgg gcatggagtc gtgaagaaga
    22141 aatcgattat gcaattaaac ataatatccc tgtatcaatc aaccatgatt caccttattc
    22201 tatcgatcaa aatctatggg gcagagcgaa tgaatgtggt attttagaag atccttatgc
    22261 tgcgccacca gaggatgcgt tcgatctaac aaatgcttta gaagaaacac cagatactgc
    22321 tgatgaaatc attttaacgt ttgataaagg catcccagtt caaattgatg gcaaaacata
    22381 tgaattagac gatttaattt taacgttgaa tgcattagct ggtaagcatg gtatcggaag
    22441 aattgaccat gtagaaaata gacttgtagg tatcaaatca agagaaattt atgaggcacc
    22501 tgctgcagaa gttattttaa aagcgcataa agcattagaa acgattacgt taacgaaaga
    22561 tgtcgcacac tttaaaccaa tcattgagaa gcaatttgct gaacaactat acaatggact
    22621 ttggttctca cctttaactg atagcttgaa attatttatt gatagtactc agcaatacgt
    22681 aagtggtgat gtcagaatta aattattcaa aggtaatgcc atcgtgaatg gtagaaaatc
    22741 accttacaca ttatatgatg aaaaattagc aacttataca aaagaagatg catttaatca
    22801 agacgctgct gttggcttta tcgatatcta tggtttacct actcaagtaa atgcgatgtt
    22861 gcatggaggc tatagcaatg agcaataaag cttggggcgg tagatttgaa gtacaacctg
    22921 aagagtgggt tgacgacttt aacgcatcca ttacttttga tcaaacgctc atagatcaag
    22981 atatcgaagg cagcattgca catgcaacta tgcttgcgaa tcaaggcatt attagtcaac
    23041 aagacagcga acaaattata caaggactaa aatctattca acatgattat catcaagatc
    23101 aaattcaatt tagtgcatca ttagaagata ttcatttaaa tattgaacat gaattaatta
    23161 aacgtatcgg tgatgctggt ggtaagttgc atactggacg cagtagaaac gatcaagttg
    23221 caacagacat gcacttgtac actaagaaac aagtgcaaga tatcatcgca ttaattaagt
    23281 cgttacaaag tgtaattgta gacatcgctt ccaataatgt tgatacaatt atgcctagtt
    23341 atactcattt acagcgtgca cagccaattt catttgcaca tcatattatg acttattttt
    23401 ggatgttaca acgagaccaa caacgatttg aagatagttt aaaacgaatc gatattaatc
    23461 ctttaggtgc agcagcctta agtggtacca cataccctat cgatagacac gagacaacag
    23521 cattgttgaa ctttggcagt ctctatgaga atagcctaga tgctgttagt gacagagact
    23581 atattattga aacattgcat aatatttctt taacgatggt tcacttatca cgctttgcag
    23641 aggaaattat tttctggtcc acagacgaag ctaaattcat tacattatca gatgcatttt
    23701 caactggctc atctattatg ccacaaaaga aaaatcctga tatggcagaa ttaattagag
    23761 gtaaagttgg tcgaacgact ggtcatttaa tgagcatgct tatgacttta aaaggattac
    23821 ctctagcata taacaaagat atgcaggaag ataaagaagg tttattcgat gctgtccata
    23881 caattaaagg ttctttacgt attttcgaag gtatgattca aacgatgaca attaataaag
    23941 aacgactcaa tcaaactgtt aaagaagatt tttcaaatgc aacggaacta gcagattatt
    24001 tagtaactaa aaatattcca tttagaactg cacatgaaat tgtaggaaaa atcgtcttag
    24061 aatgtataca acaaggtcat tatttattag atgttccttt agcaacatat caacaacatc
    24121 attctagtat tgatgccgat atttacgatt atttgcagcc tgaaaattgt ttaaaacgac
    24181 gtcaaagtta cggttcaaca ggtcaatcat cggtcaaaca acaacttgat gttgctaaac
    24241 aattactatc acaataaata cgttaatcta cctacccaca atgtctatta aaattacatt
    24301 gtgggtattt taatgctctc ttcgtcttgt tgaacatcac atttttaaga ttcctaaaat
    24361 gtttgataat tcttttaaat ttatattaca aaaatgttat aaattgtaaa agaaatgtgt
    24421 aaagcgtttt cacaagcagg tttttgtagt attttaaaat tgttagacta caaataaaga
    24481 gatgaaagga taaagactat gactaactct tcgaaaagct tcactaaatt tatggctgct
    24541 tctgctgttt ttactatggg atttttatca gtacctactg ctggcgctga acaaacaaat
    24601 caaattgcaa ataaacctca ggctattcaa tggcatacaa atttaacgaa tgagcgattc
    24661 actactatcg cacatcgtgg cgcaagtggc tatgcacccg agcatacgtt tcaagcatat
    24721 gataagagtc ataatgagtt aaaagcatct tatatcgaaa ttgatttaca acgtaccaaa
    24781 gatggccatt tagttgctat gcatgatgaa actgttaacc gtacaacaaa tggacacggt
    24841 aaagttgagg attataccct tgatgaatta aaacagttag atgcaggaag ttggtttaat
    24901 aaaaaatatc caaaatacgc aagagcaagt tataaaaatg ctaaagtacc cactttagat
    24961 gaaattttag aacgttatgg cccgaatgca aactattata ttgaaacaaa gtcacctgat
    25021 gtatacccag gaatggaaga acaattatta gcttcattga aaaagcatca ccttttaaat
    25081 aacaataaat taaaaaatgg acatgtaatg attcaatcat tttctgacga aagtttaaag
    25141 aaaattcatc gtcaaaataa gcatgtgcca ttagtaaaat tagttgataa aggtgaacta
    25201 caacaattta acgaccaacg cttaaaagag atacgctctt atgcgattgg attaggtcct
    25261 gattatacag atttaactga acaaaatacc catcatttaa aagacttagg atttatagta
    25321 catccttata cagtgaatga aaaagctgat atgttacgat taaataaata tggcgttgat
    25381 ggtg
//
LOCUS       DAKTIP010000016        24587 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAKTIP010000016 DAKTIP010000000
VERSION     DAKTIP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.016"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(63..776)
                     /locus_tag="O0305_000524"
     CDS             complement(63..776)
                     /locus_tag="O0305_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273682.1"
                     /translation="MNKERNIIIAKNIRKFLNDSNMSQKKLAELINIKPSTLSDYLNL
                     RSNPSHGVIQRIADVFEVGKSDIDTTYKDDNDITSIYNKLTPPRQENVLNYANEQLEE
                     QNSKGDNVVDINSYKQEKTPVNVNGCVSAGVGERLHDETLFTEMVKGPIPTHDLALKV
                     NGDSMEPMFKDGEIIFVEKTHNIKNGQIGIFIIEEEAYVKKVFVEDDRLTLVSLNKDY
                     DDLHFYRNESVRLIGKVIL"
     gene            739..912
                     /locus_tag="O0305_000525"
     CDS             739..912
                     /locus_tag="O0305_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0273683.1"
                     /translation="MFLAIIIFLSLFILLTSFFLIIRNFHIIVKFFTKKTYLMLTIRK
                     FRIILGLRKAVTT"
     gene            909..1172
                     /locus_tag="O0305_000526"
     CDS             909..1172
                     /locus_tag="O0305_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273684.1"
                     /translation="MKTLKELRTDYGLTQKELGDLFKVSSRTIQNMEKDSTNIKDSLL
                     SKYMSAFNVKYDDIFLGNEYENFVFTNDKKKSIILAFKEKQTS"
     gene            1188..1403
                     /locus_tag="O0305_000527"
     CDS             1188..1403
                     /locus_tag="O0305_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCY0273685.1"
                     /translation="MNIQEATKLAMEKGISIRRENQDVYGILPTNLQRYQCLVVSRHY
                     KKKRQTAAGRWQPSADDLIADDWILDY"
     gene            complement(1392..1721)
                     /locus_tag="O0305_000528"
     CDS             complement(1392..1721)
                     /locus_tag="O0305_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273686.1"
                     /translation="MTNEAKFVLLQLYSIYLDRIDEGMSKRSASYFGSDESSFNAFFL
                     DFNFEDYIDAVLELKHRDFVIASAEDGGFLEMALSREGIAYSESESKKDYKTLMDLIR
                     DLKKLII"
     gene            1772..2524
                     /locus_tag="O0305_000529"
     CDS             1772..2524
                     /locus_tag="O0305_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCY0273687.1"
                     /translation="MQALQTNSNIGEMFNIQEKENGEIAISGRELHQALEVKTAYKDW
                     FPRMLKYGFEENTDYTAIAQKRATAQGNMTHYIDHALTLDTAKEIAMIQRSEPGKRAR
                     QYFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVG
                     ELAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITH
                     SDGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            2540..2716
                     /locus_tag="O0305_000530"
     CDS             2540..2716
                     /locus_tag="O0305_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273688.1"
                     /translation="MNEEKLKMILLLLEDIPRDEWNRLVNEVNNQYSYQADKVGLASD
                     NCQQIANNYKHYGF"
     gene            complement(2713..2943)
                     /locus_tag="O0305_000531"
     CDS             complement(2713..2943)
                     /locus_tag="O0305_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273689.1"
                     /translation="MHKRLLTQYLDKEIVTSLDLHLINGEVIKVQEHMKDAESKTLHI
                     IYPKDRVVSLDHVLYFDINVKGEKNNDSPYPS"
     gene            3002..3322
                     /locus_tag="O0305_000532"
     CDS             3002..3322
                     /locus_tag="O0305_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCY0273690.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDESFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            3319..3480
                     /locus_tag="O0305_000533"
     CDS             3319..3480
                     /locus_tag="O0305_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCY0273691.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPLLYFTTAWSIAGFASIGTFI
                     YYKEYFYEE"
     gene            3575..3835
                     /locus_tag="O0305_000534"
     CDS             3575..3835
                     /locus_tag="O0305_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001794769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0273692.1"
                     /translation="MTKNYKDMTQDELRGLLAEKNAELFDLASEIDEETEFDVLLFSN
                     VGISNGDFTPSSHCVIGNVVDIANLLKRRAVYRDIADVIKMR"
     gene            3875..4135
                     /locus_tag="O0305_000535"
     CDS             3875..4135
                     /locus_tag="O0305_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0273693.1"
                     /translation="MYYKTGDVCRKIINVDGFDFQLRVKKRAYSVEMVVLDHEGNSID
                     GLLVSDENDLYTALDILKQSVYEWIENNTDEQDKLMNLVMKW"
     gene            4144..4407
                     /locus_tag="O0305_000536"
     CDS             4144..4407
                     /locus_tag="O0305_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273694.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            4416..6359
                     /locus_tag="O0305_000537"
     CDS             4416..6359
                     /locus_tag="O0305_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0273695.1"
                     /translation="MKINKLTISNFAGIKEEKFNFDGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQKLPVEQVDMAREKALQKFNASKSKELETIQTS
                     INHIISEGKKIKLIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDKVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            6361..7281
                     /locus_tag="O0305_000538"
     CDS             6361..7281
                     /locus_tag="O0305_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCY0273696.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            7362..7979
                     /locus_tag="O0305_000539"
     CDS             7362..7979
                     /locus_tag="O0305_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0273697.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYFGGL"
     gene            7980..8450
                     /gene="ssb"
                     /locus_tag="O0305_000540"
     CDS             7980..8450
                     /gene="ssb"
                     /locus_tag="O0305_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0273698.1"
                     /translation="MLNRTILVGRLTRDPELRTTQNGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIELNDDDLPF"
     gene            8480..9373
                     /locus_tag="O0305_000541"
     CDS             8480..9373
                     /locus_tag="O0305_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0273699.1"
                     /translation="MTGWISIDRSIQNHWLFKEKRTFSKFEAWIYLLMEANHSKAKVP
                     IGNQIVTVERGQRLTSILTLSDLFNWSRFKVKTFLDLLESDGMLEVKTTSKYTLITIV
                     NYDFYQSEQGRNQHQNDIKPTSKQHQSNINPTSKQHQTNTNNNDNKDNNEKNVNNEKK
                     KTTAFDFFQDNGFGFITSYNLDDLNYYLDSFENDSDEIVTASLKIAKDRNKVTWGYAK
                     SILNTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPE
                     VDENLEKDREAFIKRLNSKWE"
     gene            9380..9598
                     /locus_tag="O0305_000542"
     CDS             9380..9598
                     /locus_tag="O0305_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273700.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGLKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            9607..10011
                     /locus_tag="O0305_000543"
     CDS             9607..10011
                     /locus_tag="O0305_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCY0273701.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFSTKGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELEFYFTPPKSWSKKKKTQAIGQLKVTKPDIDNLMKTVLDACN
                     NYLWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            10024..10392
                     /locus_tag="O0305_000544"
     CDS             10024..10392
                     /locus_tag="O0305_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273702.1"
                     /translation="MARKARIVTINDKPYRFTKSEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKRKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            10396..10638
                     /locus_tag="O0305_000545"
     CDS             10396..10638
                     /locus_tag="O0305_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCY0273703.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQQGLEYEEQKQLTLF"
     gene            10647..11018
                     /locus_tag="O0305_000546"
     CDS             10647..11018
                     /locus_tag="O0305_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0273704.1"
                     /translation="MKVNYETGFQIGVMEARLKKMRKQRDEYKKQRDELIGDIAKLRE
                     RNEELENMWRTLKNELFGRYEFYRFRLSELQIESRANKEVAIYRRAEINLSVILCRMD
                     KLDGTNEFYEFLGQMEDDTNE"
     gene            11011..11247
                     /locus_tag="O0305_000547"
     CDS             11011..11247
                     /locus_tag="O0305_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0273705.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEIRE
                     AINAQWVEYPEDWASEVLREVRELEYEEEQENEN"
     gene            11237..11479
                     /locus_tag="O0305_000548"
     CDS             11237..11479
                     /locus_tag="O0305_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273706.1"
                     /translation="MKINESLKKLKEKGYKENEDKAIFNLADGTLEIYIDHDEKTIIT
                     EFHDLKVFVSEDLKDKSMESVMYELAGIDEEDKEND"
     gene            11472..12005
                     /locus_tag="O0305_000549"
     CDS             11472..12005
                     /locus_tag="O0305_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCY0273707.1"
                     /translation="MTNILQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     DMSNFGRSPAGIDGKYARLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            12042..12287
                     /locus_tag="O0305_000550"
     CDS             12042..12287
                     /locus_tag="O0305_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273708.1"
                     /translation="MSDMLEIFLIGFGVYLFYRIAIIFLKSKKTIHTNIYEMLMLATI
                     FMISTFAYKHQKTHILIAFLVMFFMSKLKQVQGSYEE"
     gene            12284..12490
                     /locus_tag="O0305_000551"
     CDS             12284..12490
                     /locus_tag="O0305_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0273709.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     EKQVKRDAVIKVGQLFENIRECGK"
     gene            12487..12873
                     /locus_tag="O0305_000552"
     CDS             12487..12873
                     /locus_tag="O0305_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273710.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGITALLLVVALPIYTVASYQNK
                     EVHQGTITDKYNKRQDKEDKFYIVLDDKQVIENSDLFFKGKFDSADIQARLKVGDKVK
                     VKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            12870..13019
                     /locus_tag="O0305_000553"
     CDS             12870..13019
                     /locus_tag="O0305_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0273711.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            13019..13219
                     /locus_tag="O0305_000554"
     CDS             13019..13219
                     /locus_tag="O0305_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0273712.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            13247..13663
                     /locus_tag="O0305_000555"
     CDS             13247..13663
                     /locus_tag="O0305_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0273713.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            13895..14194
                     /locus_tag="O0305_000556"
     CDS             13895..14194
                     /locus_tag="O0305_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0273714.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            14324..14668
                     /locus_tag="O0305_000557"
     CDS             14324..14668
                     /locus_tag="O0305_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273715.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            14665..16326
                     /locus_tag="O0305_000558"
     CDS             14665..16326
                     /locus_tag="O0305_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0273716.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            16342..17529
                     /locus_tag="O0305_000559"
     CDS             16342..17529
                     /locus_tag="O0305_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0273717.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGENDEKEKDENTLKGGDEDE
                     SGD"
     gene            17513..18250
                     /locus_tag="O0305_000560"
     CDS             17513..18250
                     /locus_tag="O0305_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0273718.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            18274..19419
                     /locus_tag="O0305_000561"
     CDS             18274..19419
                     /locus_tag="O0305_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0273719.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            19439..19723
                     /locus_tag="O0305_000562"
     CDS             19439..19723
                     /locus_tag="O0305_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273720.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            19713..19997
                     /locus_tag="O0305_000563"
     CDS             19713..19997
                     /locus_tag="O0305_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCY0273721.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            19981..20343
                     /locus_tag="O0305_000564"
     CDS             19981..20343
                     /locus_tag="O0305_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCY0273722.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            20340..20744
                     /locus_tag="O0305_000565"
     CDS             20340..20744
                     /locus_tag="O0305_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCY0273723.1"
                     /translation="MSVKVIGDKALERELENRFGTQAMLKVQDRALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIENGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            20741..21145
                     /locus_tag="O0305_000566"
     CDS             20741..21145
                     /locus_tag="O0305_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273724.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDERAYSYIVQIDVFVKFNEKYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSTRVYEGIFYEEE"
     gene            21149..21793
                     /locus_tag="O0305_000567"
     CDS             21149..21793
                     /locus_tag="O0305_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0273725.1"
                     /translation="MVVKQASAPKAYFNITGLGFAKLTKEGAKLEYSDITKTRGLQKI
                     GVETGGDLKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEEYDEYGVYKET
                     QGKQNNYVAIWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHSGNGEKGEEAFLKKILGEEYTGNVTEDNGETL"
     gene            21820..22059
                     /locus_tag="O0305_000568"
     CDS             21820..22059
                     /locus_tag="O0305_000568"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCY0273726.1"
                     /translation="MKSVNIPDSFKTLKVGNTYDLNVVVEPSNQSKLLKYTTDQTNIV
                     SINSDGQVTAEAQGIATVKAYVGNMSDTITINVEA"
     gene            22109..22459
                     /locus_tag="O0305_000569"
     CDS             22109..22459
                     /locus_tag="O0305_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273727.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFAVKDLKERMHAPDGMNALREQVVFITQG
                     QQTEETRNFIQNMK"
     gene            22510..22647
                     /locus_tag="O0305_000570"
     CDS             22510..22647
                     /locus_tag="O0305_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273728.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            22704..>24587
                     /locus_tag="O0305_000571"
     CDS             22704..>24587
                     /locus_tag="O0305_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0273729.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQ"
BASE COUNT         9569 a         3496 c         4808 g         6714 t
ORIGIN      
        1 tcattcatag tttcaaagtc taagactgca atatcatatt ttttcatttg caagtgctcc
       61 ttttataaaa taacttttcc aattaacctc acactttcat ttctataaaa gtgtagatcg
      121 tcgtaatctt tatttagtga aactagagtc aatctatcat cttcaacaaa gactttctta
      181 acgtacgctt cttcttcaat gatgaatata ccaatttgtc cattctttat attgtgagtt
      241 ttctccacaa atatgatttc gccatcttta aacataggtt ccatagaatc accatttact
      301 tttaacgcta aatcgtgtgt ggggataggt cctttaacca tttcagtaaa tagcgtttca
      361 tcgtgtaaac gttctcctac accagcagag acgcaaccat tgacgttaac tggagttttc
      421 tcctgtttat atgaattaat atctacaacg ttatctcctt tagaattctg ttcttccaat
      481 tgttcatttg catagttaag tacgttttct tggcggggag gtgtgagttt gttgtatatg
      541 gaagtgatgt cgttatcgtc tttgtatgta gtatctatgt cgcttttacc aacctcgaaa
      601 acatcagcta tcctttgtat aacgccgtga gaggggttgg aacgtaaatt taaataatcg
      661 cttaaagtag atggttttat gttaatgagt tcagcaagtt tcttttgaga catatttgaa
      721 tcgttgagaa attttctaat gtttttggct ataataatat ttctttcttt gttcatatta
      781 cttacctcct tttttcttat tatacgaaat tttcatatca tagtaaagtt ttttacgaaa
      841 aaaacgtatt taatgttgac aatacgaaaa tttcgtatta tattaggttt acgaaaggcg
      901 gtgacaacat gaaaacatta aaagagttga ggactgatta cggattgact caaaaagagt
      961 taggagattt atttaaggtc tcatcacgta caattcaaaa tatggaaaaa gactctacaa
     1021 acattaaaga tagtttactt tctaagtata tgagtgcttt taatgttaaa tatgatgata
     1081 tttttttagg taatgaatac gaaaatttcg tatttacgaa tgataaaaag aaatcaatta
     1141 ttttagcatt taaagaaaaa caaacatctt aataggagga ataacaaatg aacattcaag
     1201 aagcaacgaa gctagcgatg gagaaaggaa taagtataag gagagagaat caagatgtgt
     1261 atgggatatt accaactaat ttgcagcgtt atcaatgcct agtcgtatct agacactata
     1321 agaaaaaaag acaaaccgcc gccggaaggt ggcagcctag cgcagacgat ttaatagcag
     1381 atgattggat tttagattat taattttttc aaatctctaa ttaaatccat aagtgttttg
     1441 taatcttttt tggattctga ttctgagtag gcgatacctt ctcgagaaag agccatctca
     1501 agaaaaccgc catcttcagc agaagcaatt acaaaatctc tatgctttaa ttcaagaact
     1561 gcatcgatat agtcttcaaa attaaaatct aaaaagaaag cgttaaatga ggattcatca
     1621 ctaccgaaat aagatgcaga acgtttagac ataccttcgt caattctatc aaggtaaatt
     1681 gaataaagtt gtaaaaggac aaatttagct tcattagtca taagtcattc acctccttaa
     1741 taggagtata gcagaaagga gcataaacaa tatgcaagca ttacaaacaa attcgaacat
     1801 cggagaaatg ttcaatattc aagaaaaaga aaatggagaa atcgcaatca gcggtcgaga
     1861 acttcatcaa gcattagaag ttaagacagc atataaagat tggtttccaa gaatgcttaa
     1921 atacggattt gaagaaaata cagattacac agctatcgct caaaaaagag caacagctca
     1981 aggcaatatg acacactata ttgaccacgc actcacacta gacactgcaa aagaaatcgc
     2041 aatgattcaa cgtagtgaac ctggtaaacg tgcaagacaa tacttcatcc aagttgaaaa
     2101 agcatggaac agcccagaaa tgattatgca acgtgcttta aaaattgcta acaacacaat
     2161 caatcaatta gaaacaaaga ttgaacgtga taaaccaaaa attgtatttg cagatgcagt
     2221 agctactact aagacatcaa ttttagttgg agagttagca aagatcatta aacaaaacgg
     2281 tgtaaacatc gggcaacgca gattgtttga gtggttacgt caaaacggat tccttattaa
     2341 acgcaagggt gtggattata acatgcctac acagtattca atggaacgtg agttattcga
     2401 aattaaagaa acatcaatca cacattcgga cggtcacaca tcaattagta agacgccaaa
     2461 agtaacaggc aaaggacaac aatactttgt taataagttt ttaggagaaa aacaaacatc
     2521 ttaaaaggag gaataacaaa tgaacgaaga aaaattaaag atgattctct tacttcttga
     2581 agatatacca agagacgaat ggaatcgatt agtaaatgaa gtaaataatc aatacagtta
     2641 ccaagctgac aaggtaggac ttgccagtga taactgtcaa caaatagcaa ataactataa
     2701 gcactatgga ttttaagatg gataaggact atcgttattc ttttcacctt taacgttaat
     2761 gtcgaaatac aaaacatgat ctaaactgac aactctatct tttggataaa tgatgtgtag
     2821 agttttgctt tcagcatctt tcatatgttc ttgtacttta ataacttcac cattaattaa
     2881 atgtaaatct aaagaagtaa cgatttcttt atctaaatat tgagtgagca atctcttatg
     2941 catacttatc acctcctttc actaggagat aacaacatta tacacgaaag gaaagataga
     3001 aatgccacac attttaaacg taacagttcc aatacctgaa acacatgtac ttatcacaaa
     3061 agatgaatat gatgagctaa ttggttattc attagaccct gtatggaaca tgagtgactt
     3121 aaagaagaaa ttaaaaattg catctgatga gactatcaag gacagattac tatttcatcc
     3181 tagatttgaa aaagaactaa gagcgcaagg aattgtgcat tacccagatg agagttttaa
     3241 tcgctggaga tttaacgcaa gaaagatgaa taaattcgtc gatgagcatt tcaatgaaat
     3301 atataaggag agaataaaat gagcgacaca tataaaagct acctattagc agtgctatgt
     3361 ttcacagtct tagcgattgt gcttatgcca ttgctgtact tcactacagc gtggtcaatt
     3421 gcaggattcg caagcattgg aacattcata tactacaaag aatactttta tgaagaataa
     3481 aaaaactgct acttgcgtca acaagtaaca gtgacaaacg attaacaaaa ttaattcatt
     3541 ttcaatataa aacgaaacaa ggaggaagtc aaccatgact aaaaattata aagatatgac
     3601 tcaagacgaa ttaaggggtt tattggctga aaagaatgca gaattgtttg atttagcgag
     3661 cgaaatcgat gaagaaactg aatttgacgt tttacttttt tcaaatgtag ggattagcaa
     3721 cggagatttt acaccgtcgt cacattgtgt gattgggaat gttgttgata ttgctaattt
     3781 attgaaacgt agagctgttt accgagatat cgctgatgtt atcaaaatgc gttaaaaatt
     3841 ctagctatag ataatgacaa ggaggacgag aacaatgtat tacaaaacgg gtgacgtatg
     3901 tcgaaaaata attaatgtag atggctttga ttttcaatta agagttaaga aacgagcgta
     3961 tagcgtcgaa atggttgttc tagaccatga aggaaattca attgacgggt tactagtttc
     4021 tgatgagaac gatctataca cagctttaga tattttgaaa caaagtgttt atgaatggat
     4081 tgaaaataac acagatgaac aggacaaact aatgaactta gtcatgaaat ggtaggtata
     4141 agcatgagag atacagaaag aaatatattg aatattttta agacgttatt cgacgaatat
     4201 actttgtcaa accaacgagc attattggaa attgaacgta atcatcacgg atacttatcg
     4261 attaatttct tgcactatca cgacagttac aaaacaaaca ataagcttgt gcagatacat
     4321 gaaatcaatc cagacagcca tgaacgaata aaaaatttaa ttatcgaggt actaagaggt
     4381 catcggaaga ttaaaaaagg agcatgagga aggatatgaa aataaataaa ttaacaatat
     4441 cgaactttgc cggaatcaaa gaagaaaaat ttaattttga cggtaaagat gcaaaaatat
     4501 acggcaataa tgcgactggt aagactacaa cagcaaccgc attacaatgg ttgcttttcg
     4561 ataaaggttt ggacggttca accaaatcat ttaaccctgt acctttaaac gaaaaaaacg
     4621 aagaaaatta tgagttaatt ccgactgttt tcgcagaatt tgaaatcgac ggaaaaatta
     4681 cgacttttaa aaaagagtca catcctaaat acacaataaa tcaaaagacg aatcgcaagg
     4741 aatactcacg aagtcgaacg aagaaacaat atatcaatga tgaatcaata aaagtaaagg
     4801 attataaagc tcgtattgat gaacttattg atgaagatgt attcaagtta attacgaacc
     4861 ctcaagcatt taacttacta gattggaaga agcgaagaag tttgttgttt gaaattgcta
     4921 aaccaatcaa tgatgaggat gtcattaaaa caaatgatga ttttaaagaa ctaaataata
     4981 ttcttggaga tcatgaaatt gaaacaaaga aaaagattct tacggacaag ataaaacaga
     5041 ttaacaaaga tatcaaagat attccgatac gtattaacca aacacaacaa aataagcagg
     5101 atgtaccaga attcgataat gatagatacg caattatcaa acaagaaatt gagcaacttg
     5161 aaaatgagcg tatagatatt caaaacggta aggaagaaat taatttacgt aatcaattag
     5221 ctgataaaca atcagaattg aaacgcatag aagacaataa cagcgcaagt aatgagaaca
     5281 aaatccatgc tttaacaaat gaattacacg ttgaaaatgg aacggtagca aaccttaaaa
     5341 cgagattaaa gcaaaacaaa caacaaatca cgcatgaaga aaatagacgt aatcaattat
     5401 tagaaaatca taaaggatta aaaagtgatt tagaaaaagc taaaaatcaa aaatttgaat
     5461 atcttgatga caatgtatgt agttgttgtg gtcaaaagtt accagttgaa caagtggata
     5521 tggcaagaga aaaagcattg cagaaattca atgctagcaa atcgaaagaa ttagaaacaa
     5581 tacaaacatc tatcaatcac attatttcag aaggcaagaa aataaagcta atcatcgaga
     5641 agttagagga tgacaataat aatcttcaaa ttaaaatcaa cgaagcagaa gagcgttcag
     5701 caagaataca aaacaaaatt aataagttga aaacaactca cgttgacgtt acgcaaactg
     5761 acgaatacaa agcagtaatg ttagagataa atgagattaa tcaaaaacgc tctaacatca
     5821 ggaaaactat tcaagataaa gtttcaggaa tagatgacaa aataagcgaa cttactcaag
     5881 aaaaatcaga aattgaagtg tcaagatcaa tcgaaaaatc aaataaacat ctagatgatg
     5941 ttatttctga attaagaaat gaagaagaca gattattgga tgaaaaagaa aagtattcac
     6001 atgaccttta tatcttaaaa gaatttacaa caacaaaagt caaaatgctt actgaaaaca
     6061 tcaataatga atttgaaatt gctgaattta agctattcaa taccttagtt aacggcgaat
     6121 tagaagaaac atgttccaca acggttaacg gcgtcgaata cgacagcggt ttaaataacg
     6181 cctcaagaat taatgttggc ttagatatca tcaatacact gtcaaaacat tttaaagtta
     6241 cagcgccaat atttattgat aatgctgaat cagtaacaga gcttatcaaa acagaatcac
     6301 aacaaattca attgatagta aatgaacaag ataaaaaatt aagaatggag actatataaa
     6361 atgactgaaa ataataaatt acaaactatt gaacaacaat tagtacaaga aaagaacgta
     6421 tctgacaacg tattaaacaa agtgagagtt ttagagtcac aaggcaattt ggaattgcca
     6481 aatgattatt caccaagtaa tgccatgaaa caagcatggt tacaaatcag ccaagataac
     6541 aaattaatga gttgtaacga tacaagcaaa gcaaatgcct tattagacat ggtaacgcaa
     6601 ggtttaaatc cagctaaaaa tcaatgctac tttattcctt acggcaacaa aatgcagtta
     6661 caacgtagct atcacggtaa tgtaatgatg ttaaaacgtg atgcaggtgc tcaagatgtt
     6721 gttgctcaag tgatttataa aggcgataca ttcaagcaag aaatgggaga aacaggacgt
     6781 atcaaagcga ttaaacacga acaagacttc tttaacatcg acaaagaaaa cattatcggt
     6841 gcgtactgca caatcgtatt taatgatgga cgagataact atattgaagt catgactatt
     6901 gaacaaatta aacaagcatg gatgcagtca tcaatgatta aagatgaaaa agcattacaa
     6961 aattctaaaa cacataataa tttcaaagaa gaaatggcta aaaaaacagt tatcaataga
     7021 gctgctaaac gttatatcaa cacatcaaca gatagcaatc ttttcaaata cgcacaagaa
     7081 tccgaacaac gtcaacgcaa agaagtgttg gacgcagaag ttgaagaaaa tgcaaatcaa
     7141 gaacaattgg actttgaaca accagttctt gaagaagcac aatacacaga attagaaaat
     7201 gataagccta ttgatgtatc tgactttgaa gaaataaaag aacctgcaac agaaaaagaa
     7261 agcgaagaag agccatttta attgaaacaa tagcaactgg ttcaagtggt aactgctacg
     7321 tcttaaatga tggacgtact acgttactac ttgaggcagg tataaaattt gaacgtgttc
     7381 aaaagcattt caaatataaa acaagacata tagcagggtg tcttatcaca cacgaacatg
     7441 gtgatcatgc aaagtataca aagcagtttg tcgacaatgg tgtaatcagc tatatgactg
     7501 ctggaacaca acaagctatg aattttgaaa gtcatcgctt atgcacgatt aaggcaaagc
     7561 aagagctgcg aataggtaca tggtcaattc taccgtttga catcgaacat gatgctaacg
     7621 agcctgtggc tttcttatta caaagcacac taggttataa ggttctgtat gttactgata
     7681 caaagtattt gaaatacaaa tttaatggca ttacgcacat gatgctagaa gttaattata
     7741 tctatgaaca aatgcaagaa aacataaaaa acggcagtgt gcacagcaca ttagcaaaca
     7801 gaattatgga gtctcatttt agcttagaac atgctatcgg aatgttaaaa gcaaatgatt
     7861 taactagact cgaagaaata catttaattc atttaagtag tcaaaattca aatgcaaaat
     7921 acattaaaag tgaaatacaa aaagtgacgg gcgcgcccgt ttattttgga ggtttataaa
     7981 tgctaaacag aacaatatta gttggtcgtt taactagaga cccagaatta agaaccactc
     8041 aaaatggtgt aaatgtagca tcattcacat tagcagttaa ccgtacattt acaaatgcac
     8101 aaggcgagcg cgaggcagac tttattaata tcatcgtatt taaaaaacaa gcagagaacg
     8161 ttaataaata cctatctaaa ggatcgttgg cgggcgtaga tggtaggtta caaacgcgga
     8221 actatgaaaa taaggaaggt caacgtgtat acgttacgga agttattgct gatagtattc
     8281 aatttttaga accgaaaaac tcaaatgaca ctcaacaaga tttatatcaa caacaagtac
     8341 aacaaacacg tggacaatcg caatattcaa ataacaaacc agtaaaagat aatccgtttg
     8401 cgaatgcaaa tggtccgatt gaactaaatg atgatgattt accattctaa tttaaccggt
     8461 ttgaaagtga ggtgtgtata tgactggttg gataagtatt gatcgctcaa ttcaaaatca
     8521 ttggctattt aaagaaaaga gaacattttc aaagtttgaa gcatggatat atttactcat
     8581 ggaagcgaat cattcaaagg caaaagtgcc tataggaaac caaattgtaa ccgttgaaag
     8641 aggacaaaga ttaacatcta ttttgacctt gtctgacctt tttaactggt cacgatttaa
     8701 agtgaaaacc ttccttgact tactcgagag tgatggaatg ttagaagtca aaacaacatc
     8761 aaaatatacc cttataacca ttgtcaatta tgacttttat caaagtgagc agggcaggaa
     8821 ccaacatcaa aacgacatca aaccaacatc aaaacaacat cagtcaaaca tcaacccaac
     8881 atcaaaacaa catcaaacca acacaaacaa taatgataat aaagataata atgaaaagaa
     8941 tgtgaataat gagaagaaga agacaaccgc cttcgacttc ttccaagata acggattcgg
     9001 tttcataact tcttacaatt tagacgattt aaattattat cttgattcat ttgaaaatga
     9061 ttcagatgaa atagttaccg catcacttaa aatcgctaaa gacagaaaca aagttacttg
     9121 gggatatgct aaaagcattt tgaatacatg gcttaatgca aacttgaaat ctattgaaca
     9181 agtacgtgca tttgaaaagc aacaacttga aagcaaaaaa caaaattata aacctttcgt
     9241 taaacaatca aaagaaaaaa cacccaaatg gctcacagac agcacgagag aaacgaaaac
     9301 gccggaagta gatgaaaacc ttgagaaaga cagagaagct tttattaagc gtctaaatag
     9361 caaatgggag tgattgaaaa tggatgcatt tgataaatac tatctatttg atcatgacgg
     9421 caacaaaatg ttttcagtta caccacattt taaagatgga cggcatttag ttgttggatt
     9481 aaaacacaca aaattcaatg gtcgacgttg gtacttagat gattatgaat taaaaacact
     9541 tattgataat gaacaaatgg agttaggaca ccaaacaagc ttatttgaat atatatgagg
     9601 gattacatgg agatagaaat taaatttaat gaagtgttta atgcgccgat gggctcgcct
     9661 cgaccgcgtt ttagcacaaa aggtagatat gcacacacat atatgcctac aaaatataca
     9721 gaacataaaa aatatttaca aaatcaaatg ccaaagctaa atctagaaaa tgcattaaaa
     9781 attgaattag agttttactt tactccacct aaatcatggt cgaagaaaaa gaaaactcaa
     9841 gcgattggac aattaaaagt aactaaacca gatatcgata atttgatgaa gacagttcta
     9901 gacgcttgta acaattattt gtggaaagac gataaccaaa ttacagaaat aactagctca
     9961 aagcgttatg gaattgagcc caaaataatc atacgaatag aagaaatata agaggtggat
    10021 aaaatggcga gaaaagcaag gattgtaaca ataaatgata aaccatatag gtttaccaaa
    10081 tctgaaatgg aattaataga aagtcacggt ataaccgctg gaatggtttc taagagagta
    10141 aaagacggtt gggaactaca tgaagcaatg gacgcaccag aaggtacgcg tttaagcgag
    10201 tacagagaaa agaaaacaat agaaagactg gaacaagcta gactcgaacg caaattggaa
    10261 agaaagcgaa agaaagaggc tgagctaaga agaaagaagc cacatttgtt taatgtacca
    10321 cagaaacatc caagaggacg ttatgcgtgc tacctgatgg aaaacgacat attcgtgaaa
    10381 gttaagaagt agatcatgac agataacgca cgcaaagaat acctaaatca attctttgga
    10441 tctaagagat atctgtatca ggataacgaa cgagtggcac atattcatgt agtaaacggc
    10501 acttattact ttcatgggca tatcgtacca ggttggcaag gcgtgaaaaa gacatttgat
    10561 acagctgaag agcttgaaac atatataaag caacagggtt tggaatatga ggaacagaag
    10621 caactaactt tattttagag gaggttatga aagtgaacta tgaaacagga ttccaaatag
    10681 gcgtaatgga agctaggttg aagaagatga gaaaacaacg tgatgagtac aagaagcaac
    10741 gtgacgagct tattggggat atagctaagt taagagagcg taacgaagag ctggagaaca
    10801 tgtggcgcac gcttaaaaat gaattgtttg gaagatacga attttaccgt tttagactta
    10861 gcgaactaca gattgagagc agagcgaaca aggaagtagc tatatataga agagctgaaa
    10921 tcaacttaag tgttatattg tgccgaatgg acaaactaga cggaacaaat gagttctacg
    10981 aatttttagg gcaaatggag gatgacacaa atgaataacc gcgaacaaat cgaacaatca
    11041 gttatcagtg ctagcgcgta taacggcaat gacacagagg gattgctaaa agagattgaa
    11101 gacgtgtata agaaagcgca agcgtttgat gaaattcgcg aagctattaa tgcgcaatgg
    11161 gttgagtatc cagaagactg ggcgtcagag gttttgagag aagtaagaga gcttgaatat
    11221 gaggaggaac aggaaaatga aaattaatga atctttgaag aaattaaaag aaaaaggata
    11281 caaagaaaat gaagataaag ctatttttaa tttagctgat ggtacgctag aaatttacat
    11341 cgaccatgac gaaaaaacaa taattacaga attccatgat ttaaaggtat tcgtgtcaga
    11401 agatttaaaa gataaaagta tggagagcgt tatgtatgaa ttggctggaa ttgatgagga
    11461 ggacaaggaa aatgactaac atcctacaag taaagctatt atcagaaaac gctagaatgc
    11521 cagaacgaaa tcataagacg gatgcaggtt atgacatatt ctcagctgaa actgtcgtac
    11581 ttgagccaca agaaaaggca gtgatcaaaa cagatgtagc tgtaagcata ccagagggct
    11641 atgtcggact attaactagc cgtagtggtg taagtagtaa aacgcattta gtgattgaaa
    11701 caggcaagat agacgcggga tatcacggca atttagggat taatatcaag aatgataatg
    11761 aaacgttaga gagtgaggat atgagtaact ttggtcggag tcctgctggg atagatggaa
    11821 agtatgcccg actacctgta acagataaaa ttttatgtat gaatggtagt tatgtcataa
    11881 acaaaggcga caaactagct caattggtta ttgtgcctat atggacacct gaactaaagc
    11941 aagtggagga attcgagagt gtttcagaac gtggagcaaa aggcttcgga agtagcggag
    12001 tgtaaagaca tattagatcg agtcaaggag gttttgggga agtgagcgac atgttagaaa
    12061 tatttttaat agggtttggc gtttatctct tttatcgcat agcaattatt tttcttaaga
    12121 gtaaaaagac tatacacaca aacatatatg aaatgttaat gcttgctact atctttatga
    12181 tatctacatt tgcttataaa catcaaaaga cgcatatctt aatagcattt ttagtaatgt
    12241 tttttatgag taagctcaaa caagttcaag ggagctatga ggaatgacac aatacttagt
    12301 cacaacattc aaagattcaa caggacgtaa acatacacac ataactaaag ctaagagtaa
    12361 tcaaaggttt acagttgttg aggcagagag taaagaagaa gcgaaagaga agtacgagaa
    12421 acaagttaaa agggatgcag ttattaaagt gggtcagttg tttgaaaata taagggagtg
    12481 tgggaaatga ttaaaaaact taaaaatatg gatgggttcg acatctttat tgttggaata
    12541 ctgtcattat tcggtataac cgcattgcta cttgttgtcg cattgcctat ctatacagtg
    12601 gctagttacc aaaacaaaga agtacatcaa gggacaatta cagataaata taacaaaaga
    12661 caagataaag aggacaaatt ctatattgta ttagatgata aacaagtcat cgaaaactca
    12721 gacttattct tcaaaggaaa gtttgatagc gcagacatac aagctaggtt aaaagtaggt
    12781 gataaagtaa aagttaagac gattggatat agaatacact ttttaaattt atatccggtc
    12841 ttatacgaag taaagaaggt agataaaaaa tgattaagca aatactaaga ttattattct
    12901 tactagcgat gtatgagtta ggtaagtatg taactgagca agtatatatt atgatgacgg
    12961 ctaatgatga tgtagaggcg ccgagtgatt acgtctttcg agcggaggta agtgagtgat
    13021 gtggattact atgactattg tatttgctat attgctatta gtttgtatca gtattaatag
    13081 tgatcgtgca agggagatac aagcgctcag atatatgaat gattatctac ttgatgaagt
    13141 agttaaaact aaaggataca acgggttaaa agaatacagg attgaattaa agcgaatgaa
    13201 taacgatatt aaaaagtaat ttatattatc ggaggtatta cattgaatga taaagattga
    13261 gaaacacgat atcaaaaagc ttgaagaata cattcagcac atcgataact atcgaagaga
    13321 gttgaagatg cgagaatatg aattacttga aagtcatgaa ccagataatg cgggagctgg
    13381 caaaagtaat ttgccgggta acccgattga acgatgtgca ataaagaagt ttagtgataa
    13441 caggtacaat acattaagaa atatagttaa cggtgtagat agattgatag atgaaagtga
    13501 tgaggatacg cttgagttat taaggtttag atattgggat tgtcctattg gttgttatga
    13561 atgggaagat atagcacatt actttggtac aagtaagaca agtatattac gtagaaggaa
    13621 tgcactgatc gataagttag caaagtatat tggttatgtg tagcggactt ttaccctatg
    13681 taagtccgca ttaaaacagt ttattatgtt agtatcagat taatatttaa agttattaaa
    13741 tgctaatacg acgcatgaac aagaggcgca tcactatgtg atgtgtcttt ttatttatga
    13801 ggtatgaaca tgttcaaact aatagtaaat acattactac acatcaagta tagatgcgtc
    13861 ttgatactac ttaagttata taaggtgaaa cattatgatg actaaagacg aacgcatacg
    13921 attctataag tctaaagaat ggcaaacaac aagaaaaaga gtgctagaaa gagataatta
    13981 tgaatgtcaa caatgtaaga gagacggcaa gttaacgaca tatgacaaaa gcaagcgtaa
    14041 gtcgttggat gtagatcata tattatcgct agaacatcat ccggagtttg ctcatgactt
    14101 aaacaattta gaaacactgt gtattaaatg tcacaacaaa aaagaaaaga gatttataaa
    14161 aaaagaaaat aaatggaaag acgaaaaatg gtaaataccc ccgggtcaaa aaaatcaaaa
    14221 gcgatcaaaa tacttgggga acggggaggg gctcgacttc gcgataattt taaaaatcca
    14281 tgtatacccc ccctcttata accattttaa ggcaggtgat gaaatggaga ttatagttga
    14341 tgaaaactta gtgcttaaag aaaaagaaag gctgcaagta ttatataaag acatacctag
    14401 caataaatta aaagtagttg atggtttaat tattcaagca gcaaggctac gtgtaatgct
    14461 tgattacatg tgggaagaca taaaagaaaa aggtgactat gatttattta ctcaatctga
    14521 aaaggcgcca ccatatgaaa gggaaagacc agtagccaaa ctatttaatg ctagagatgc
    14581 tgcatatcaa aaaataatca aacaattatc ggatttattg cccgaagaga aagaagacac
    14641 agaaacgcca tctgatgatt acctatgatt agtaataaat acgttgatga atatataaat
    14701 ttgtggaaac aaggaaagat aattttaaat aaagaaagaa ttgatctctt taattatcta
    14761 caaaaacata tatattcacg agatgatgta tattttgatg aacagaaaat cgaggattgt
    14821 atcaaattta ttgaaaaatg gtattttcca acattaccat ttcaaaggtt tatcatagct
    14881 aatatatttc ttatagataa aaatacagat gaagctttct ttacagaatt tgctattttc
    14941 atgggacgtg gaggcgggaa aaacggtcta ataagtgcta ttagtgattt tctttctacg
    15001 cccttacacg gagttaaaga atatcacatc tccattgttg ctaatagtga agatcaagca
    15061 aaaacatcgt ttgatgaaat cagaaccgtt ttaatggata acaaacgaaa taagacgggt
    15121 aaaacgccaa aagctcctta tgaagttagt aaagcaaaaa taataaaccg tgcaactaaa
    15181 tcggttattc gatataacac atcaaacaca aaaaccaaag acggtggacg tgaggggtgt
    15241 gttatttttg atgaaattca ttatttcttt ggtcctgaaa tggtaaacgt caaacgtggt
    15301 ggattaggta aaaagaaaaa tagaagaacg ttttatataa gtactgatgg ttttgttaga
    15361 gagggttata tcgatgcaat gaagcacaaa attgcaagtg tattaagtgg caaggttaaa
    15421 aatagtagat tgtttgcttt ttattgtaag ttagacgatc caaaagaagt tgatgacaga
    15481 cagacgtggg aaaaggcgaa cccaatgtta cataaaccgt tatcagaata cgctaaaaca
    15541 ctgctaagta cgattgaaga agaatataac gatttaccat tcaaccgttc aaataagccc
    15601 gaattcatga ctaagcgaat gaatttgcct gaagttgacc ttgaaaaagt aatagcacca
    15661 tggaaagaaa tactagcgac taatagagag ataccaaatt tagataatca aatgtgtatt
    15721 ggtggtttag actttgcaaa cattcgagat tttgcaagtg tagggctatt attccgaaaa
    15781 aacgatgatt acatttggtt aggacattcg tttgtaagac aagggttttt ggatgatgtc
    15841 aaattagaac ctcctattaa agaatgggaa aaaatgggat tattgaccat tgtcgatgat
    15901 gatgtcattg aaattgaata tatagttgat tggtttttaa aggctagaga aaaatatggg
    15961 cttgaaaaag tcatagctga taattataga actgatattg taagacgtgc gtttgaggat
    16021 gctggcataa aacttgaagt acttagaaat ccaaaagcaa tacatggatt acttgcacca
    16081 cgtatcgata caatgtttgc gaaacataac gtaatatatg gagacaatcc tttgatgcgt
    16141 tggtttacta ataatgttgc agtaaaggtt aaacccgatg gtaataaaga atatattaaa
    16201 aaagatgaaa atagaagaaa aaccgatggg ttcatggctt ttgttcacgc attatataga
    16261 gcagacgata tagtagacaa agacatgtct aaagcgcttg atgcattaat gagtatagat
    16321 ttctaataga ggaggtgaga catgagtatt ctagaaaaga tatttaaaac taggaaagat
    16381 ataacatata tgcttgattt agatatgata gaagatctat cacaacaagc gtatgtgaaa
    16441 cgtttagcga ttgatagttg tattgaattt gttgcgcgag ctgtcgctca aagtcatttt
    16501 aaagtattgg aaggtaatag aattcaaaag aatgatgttt actacaagtt aaatataaaa
    16561 ccaaatactg acttatcaag cgatagtttt tggcaacaag ttatatataa actaatttat
    16621 gataacgagg ttttaatcgt agtaagtgac agcaaagaat tacttatcgc agatagcttt
    16681 tacagagaag agtacgcttt gtatgatgat atattcaaag atgtaacggt taaagattat
    16741 acttatcaac gtactttcac aatgcaagag gtcatatatt taaagtacaa caacaataaa
    16801 gtgacacact ttgtagaaag tctattcgaa gattacggga aaatattcgg aagaatgata
    16861 ggtgcacaat taaaaaacta tcaaataaga gggattttga aatctgcctc tagcgcatat
    16921 gacgaaaaga atatagaaaa attacaagcg ttcacaaata aattattcaa tacttttaat
    16981 aaaaatcaac tagcaatcgc gcctttgata gaaggttttg attatgagga attatctaat
    17041 ggtggtaaga atagtaacat gcctttttct gaattgagtg agctaatgag agatgcaata
    17101 aaaaatgttg cgttgatgat tggtatacct ccaggtttga tttacggaga aacagctgat
    17161 ttggaaaaga acacgcttgt atttgagaag ttctgtttaa cacctttatt aaaaaagatt
    17221 cagaacgaat taaacgcgaa actcataaca caaagcatgt atttgaaaga tacaagaata
    17281 gaaattgtcg gtgtgaataa aaaagaccca cttcaatatg ctgaagcaat tgacaaactt
    17341 gtaagttctg gttcatttac aaggaatgag gtgcggatta tgttaggtga agaaccatca
    17401 gacaatcctg aattagacga atacctgatt actaaaaact acgaaaaagc taacagtggt
    17461 gaaaatgatg aaaaagaaaa agatgaaaac actttgaaag gtggtgatga agatgaaagc
    17521 ggagattaaa ggcgtcatcg tttccaacga agataaatgg gtttacgaaa tgcttggtat
    17581 ggattcgact tgtcctaaag atgttttaac acaactagaa tttagtgatg aagatgttga
    17641 tattataatt aactcaaatg gtggtaacct agtagctggt agtgaaatat atacacattt
    17701 aagagctcat aaaggcaaag tgaatgttcg catcacagca atagcagcaa gtgcggcatc
    17761 gcttatcgca atggctggtg accacatcga aatgagtccg gttgctagaa tgatgattca
    17821 caatccttca agtattgcgc aaggagaagc gaaagatcta aatcatgctg cagaaacatt
    17881 agaacatgtt ggtcaaataa tggctgaggc atatgcggtt agagctggta aaaacaaaca
    17941 agaacttgta gaaatgatgg ctagggaaac gtggctaaat gctgatgaag ccattgaaca
    18001 aggttttgcg gatagtaaaa tgtttgaaaa cgacaatatg caaattgtag caagcaatac
    18061 acaagtgtta tcgaaagatg tattaaatcg tgtaacagct ttggtaagta aaacgccaga
    18121 ggttaacatt gatattgacg caatagcaaa taaagtaatt gaaaaaataa atatgaaaga
    18181 aaaggaatca gaaatcgatg ttgcagatag taaagtatca gcaaatggat tttcaagatt
    18241 ccttttttaa tacaaaaaat aggaggtcat aaaatgacta taaatttatc ggaaacattc
    18301 gcaaatgcga aaaacgaatt tattaatgca gtaaacaacg gtgaaccgca agaaagacaa
    18361 aatgaattgt acggtgacat gattaaccaa ctatttgaag aaactaaatt acaagcaaaa
    18421 gcagaagctg aaagagtttc tagtttacct aaatcagcac aatctttgag tgcaaaccaa
    18481 agaagtttct tcatggatat caataaaaac gttaactata aagaagaaaa acttttgcca
    18541 gaagaaacaa ttgatagaat ttttgaagat ttgacgacga atcatccgtt attagctgat
    18601 ttaggtatta aaaacgctgg tttgcgtttg aagttcttaa aatctgaaac ttctggcgta
    18661 gccgtttggg gtaaaatcta tggtgaaatt aaaggtcaat tagatgctgc gttcagtgaa
    18721 gaaacagcaa ttcaaaataa attgacagcg tttgttgttt taccaaaaga tttaaatgat
    18781 tttggtcctg cgtggattga aagatttgtt cgtgttcaaa tcgaagaagc atttgcagtg
    18841 gcgcttgaaa ctgcgttctt aaaaggtact ggtaaagacc aaccaatcgg cttaaaccgt
    18901 caagtacaaa aaggtgtatc ggtaactgag ggtgcttatc cagagaaaga agaacaaggt
    18961 acgcttacat ttgctaatcc gcgcgctaca gttaatgaat tgacgcaagt gtttaaatac
    19021 cactcaacta acgagaaagg taaatcagta gcggttaaag gtaatgtaac aatggttgtt
    19081 aatccgtccg atgcttttga ggttcaagca cagtatacac atttaaatgc aaatggcgta
    19141 tatgttactg ctttaccatt taatttgaat gttatcgagt ctacagtcca agaagcaggt
    19201 aaggttttaa cgtacgttaa aggtttatat gatggttatt tagctggtgg tattaatgtt
    19261 cagaaattta aagaaacact tgcgttagat gatatggatt tatacactgc aaaacaattt
    19321 gcttacggca aagcgaaaga taataaagtt gctgctgttt ggaaattaga tttaaaagga
    19381 cataagccag ctttagaagg taccgaagaa acactataaa attttatgag gtgataaaat
    19441 ggtgaaattt aaagttgtta gagcttttaa agacatagag cacaatcaac acaagtacaa
    19501 agtaggggag ttgtatccag ctgaagggta taacaatcct cgtgttgaat tgttgacaaa
    19561 tcaaatcaaa aataagtacg acaaagttta tatcgtacct ttagataagc tgacaaaaca
    19621 agaattatta gaactatgcg aatcattaca aaaaaaagcg tctagttcaa tggttaaaag
    19681 tgaaatcgtc gacttattga atggtgaaga caatgacgat tgatgatttg cttgtcaaat
    19741 ttaaatcact tgaaaagatt gaccataatt cagaggatga gtacttaaag cagttgttaa
    19801 aaatgtcgta cgagcgtata aaaaatcagt gcggagtttt tgaattagag aatttaatag
    19861 gtcaagaatt gatacttata cgcgctagat atgcttatca agatttatta gaacacttca
    19921 acgataatta cagacctgaa ataatagatt tttcgttatc tctaatggag gtatcagaag
    19981 atgaagaaag tgtttaaaaa acctagaatt acaactaaac gtttaaatac tcgtgttcat
    20041 ttttataagt atactgaaaa taatggtcca gaagctggag aaaaagaaga aaaattatta
    20101 tatagctgtt gggcgagtat tgatggtgtc tggttacgtg aattagaaca agctatctca
    20161 aacggaaccc aaaatgacat taaattgtat attcgtgatc cgcaaggtga ttatttaccc
    20221 agtgaagaac attatcttga aattgaatca agatatttca aaaatcgttt gaatataaag
    20281 caagtatcac cagatttgga taataaagac tttattatga ttcgtggagg atatagttca
    20341 tgagtgtgaa agtgataggt gataaagcat tagaaagaga attagaaaat cgtttcggaa
    20401 ctcaagcgat gttaaaagtt caagataggg cgctaattgc tggtgctaaa gtaattgttg
    20461 aagaagtaaa aaaacaacta aagccatcaa aagatacggg cgcattaatt aatgaggtaa
    20521 gttttagtaa acctgaatgg ataaacggaa aacgtacaat tactgttcat tggcgaggtt
    20581 ctaaagaccg ttataaaatc gtacatttaa ttgaaaatgg acacgttcaa aaagggacag
    20641 gtaaatttat caaacctaaa gctatgggtg gcgttaatag agcaataaga caagggcaaa
    20701 ataagtattt tgagacgcta aaaagggagt taaaaaaatt gtgattgata ttttgtacaa
    20761 agttcatgaa gtgattagtc aagacagaat tattagagag cacgtaaata tcaataatat
    20821 taagttcaat aaatacccaa acgtaaaaga tacggatgta cctttcatag ttattgacga
    20881 tatagacgac ccaataccta caacttatac tgacggagat gagcgcgcat atagttacat
    20941 tgtacaaata gatgtatttg tgaagtttaa cgaaaagtat aacgcgagaa tcataagaaa
    21001 taagatatct aatcgtattc aaaagttatt atggtctgaa ctgaaaatgg gaaatgtatc
    21061 caacggaaaa cctgaatata tagaagaatt taaaacatat agaagcactc gtgtctacga
    21121 gggcattttt tacgaggagg aataataaat ggtagtaaaa caagcaagtg cgccgaaagc
    21181 gtattttaac attactggtt taggtttcgc taaattaaca aaagaaggcg caaagctaga
    21241 atatagcgat attacaaaaa ctagaggatt acaaaaaatc ggtgttgaaa ctggaggaga
    21301 cttgaaaaca gcatatgctg atggtggtcc aatcgaatca gggaacacag acggagaagg
    21361 taaaatttcg ttacaaatgc atgctttccc taaagagatt cgcaaaatag tattcaatga
    21421 agagtatgat gaatacggtg tttataaaga aactcaaggt aaacaaaaca actatgtagc
    21481 aatttggttc agacaagagc gtcgagacgg cacatttaga acggttttat tacctaaagt
    21541 catgtttaca aatcctaaaa tcgatggcga aacggctgag aaagattggg atttctcaag
    21601 cgaagaggtt gaaggtgagg cacttttccc tttaattgat aataaaaagt cagtacgtaa
    21661 atatatcttt gattcagcaa acatgacaaa tcatagtgga aatggtgaaa aaggcgaaga
    21721 ggctttctta aagaaaattt taggcgaaga atatactgga aacgtgacag aggataacgg
    21781 cgaaactttg taacgcaacc ggcttcatcg gaaactgaga taaagtcggt taatatacca
    21841 gatagtttta aaacactaaa agttggcaac acatatgatt taaatgttgt agtagagcca
    21901 tctaatcaaa gtaagttatt gaaatacaca acagatcaaa cgaatattgt atcaatcaat
    21961 agtgatggtc aagtcactgc ggaagcacaa ggtattgcca cggttaaagc atatgttggt
    22021 aatatgagtg acactataac aataaatgta gaagcataag aggggacaac ccctctattt
    22081 tatttgaaaa taaggagagt attataaaat ggcaaaatta aaacgtaaca ttattcaatt
    22141 agtagaagat ccaaaagcaa atgaaattaa attacaaacg tacttaacac cacacttcat
    22201 ttcatttgaa attgtatacg aagcaatgga tttaatcgat gatattgagg acgaaaatag
    22261 cacgatgaag ccaagagaaa tcgctgacag attgatggat atggttgtaa aaatttacga
    22321 taaccaattc gcagttaaag acctaaaaga acgtatgcat gcacctgatg gaatgaatgc
    22381 acttcgtgaa caagtggttt tcattacgca aggtcaacaa actgaggaaa ctagaaattt
    22441 tatccagaac atgaaataaa gcctgaagat ttaacatata aagcaatgtt gaaaaatatg
    22501 gatactctca tgatggactt aattgaaaat ggtaaagacg ctaacgaagt tttaaaaatg
    22561 ccatttcatt atgtgctttc catatatcaa aataaaaaca atgacatttc tgaagaaaaa
    22621 gcagaggctt taattgatgc attttaacct taaccgtttg gttagggtta tttttttgaa
    22681 cttttttaga aaggaggtaa aaaatgggag aaagaataaa aggtttatct ataggtttgg
    22741 atttagatgc agcaaattta aatagatcat ttgcagaaat caaacgaaac tttaaaactt
    22801 taaattctga cttaaaatta acaggcaaca acttcaaata taccgaaaaa tcaactgata
    22861 gttacaaaca aaggattaaa gaacttgatg gaactatcac aggttataag aaaaacgttg
    22921 atgatttagc taagcaatat gacaaggtat ctcaagaaca gggcgaaaac agtgcaggag
    22981 ctcaaaagtt acgacaagaa tataacaaac aagcaaatga actgaattat ttagaaagag
    23041 aattacaaaa aacatcagcc gaatttgaag agttcaaaaa agctcaagtt gaagctcaaa
    23101 gaatggcaga aagtggctgg ggaaaaacca gtaaagtttt tgaaagtatg ggacctaaat
    23161 taacaaaaat gggtgatggt ttaaaatcca ttggtaaagg catgatgatt ggtgtaactg
    23221 cacctgtttt aggtatcgca gcagcatcag gaaaagcttt tgcagaagtt gataaaggtt
    23281 tagatactgt tactcaagca acaggcgcaa caggcagtga attaaaaaaa ttgcagaact
    23341 catttaaaga tgtttatggc aattttccag cagatgctga aactgttggt ggagttttag
    23401 gagaagttaa tacaagatta ggttttacag gtaaagaact tgaaaatgcc acagagtcat
    23461 tcttgaaatt cagtcatata acaggttctg acggtgtgca agccgtacag ttaattaccc
    23521 gtgcaatggg cgatgcaggt atcgaagcaa gtgaatatca aagtgttttg gatatggtag
    23581 caaaagcggc gcaagctagt gggataagtg ttgacacatt agctgatagt attactaaat
    23641 acggcgctcc aatgagagct atgggctttg agatgaaaga atcaattgct ttattctctc
    23701 aatgggaaaa gtcaggcgtt aatactgaaa tagcattcag tggtttgaaa aaagctatat
    23761 caaattgggg taaagctggt aaaaacccaa gagaagaatt taagaagaca ttagcagaaa
    23821 ttgaaaagac gccggatata gctagcgcaa ctagtttagc gattgaagca tttggtgcaa
    23881 aggcaggtcc tgatttagca gacgctatta aaggtggtcg ctttagttat caagaatttt
    23941 taaaaactat tgaagattcc caaggcacag taaaccaaac atttaaagat tctgaaagtg
    24001 gctccgaaag atttaaagta gcaatgaata aattaaaatt agtaggtgct gatgtatggg
    24061 cttctattga aagtgcgttt gctccagtca tggaagaatt aataaaaaag ctatcagtcg
    24121 ctgtcgattg gttttcgaat ttaagtgatg gttctaaaag atcaattgtt attttcggtg
    24181 gtattgctgc tgcaattggt cctgtagttt ttgggttagg tgcatttata agcacaattg
    24241 gtaatgcagt aactgtatta gccccactat tagctggtat tgcaaaggct gatggattaa
    24301 ttagtttttt atcgactaaa gtacctatat taggaactgt cttcacagct ttaactggtc
    24361 caattggcat tgtattaggt gttttagctg gcctagcagt cgcatttaca attgcttata
    24421 agaaatctga aacatttaga aattttgtta atgatgcaat tgaaagtgtt aaacaaacat
    24481 ttagtaattt tattcaattt attcaacctt tcattgattc cgttaaaaac gtctttaaac
    24541 aagcggtttc agcaatcgtt gatttcgcta aagatatttg gagtcaa
//
LOCUS       DAKTIP010000017        24090 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAKTIP010000017 DAKTIP010000000
VERSION     DAKTIP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.017"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(48..167)
                     /locus_tag="O0305_000572"
     CDS             complement(48..167)
                     /locus_tag="O0305_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273730.1"
                     /translation="MGGCPSVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            complement(247..387)
                     /locus_tag="O0305_000573"
     CDS             complement(247..387)
                     /locus_tag="O0305_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273731.1"
                     /translation="MSKAYRLYNNIQDDTKQPMAHAVGCFFRCVCHGQHFDVGIPLQA
                     WE"
     gene            complement(391..990)
                     /locus_tag="O0305_000574"
     CDS             complement(391..990)
                     /locus_tag="O0305_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCY0273732.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARTEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            1170..2807
                     /locus_tag="O0305_000575"
     CDS             1170..2807
                     /locus_tag="O0305_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0273733.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIALSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            3415..4767
                     /locus_tag="O0305_000576"
     CDS             3415..4767
                     /locus_tag="O0305_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCY0273734.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTNDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
     gene            complement(4829..6016)
                     /locus_tag="O0305_000577"
     CDS             complement(4829..6016)
                     /locus_tag="O0305_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273735.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(6412..7188)
                     /gene="fetB"
                     /locus_tag="O0305_000578"
     CDS             complement(6412..7188)
                     /gene="fetB"
                     /locus_tag="O0305_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCY0273736.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            complement(7181..7843)
                     /locus_tag="O0305_000579"
     CDS             complement(7181..7843)
                     /locus_tag="O0305_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0273737.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLFSPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVNNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNTEELNQ
                     HE"
     gene            complement(8091..9167)
                     /locus_tag="O0305_000580"
     CDS             complement(8091..9167)
                     /locus_tag="O0305_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0273738.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAIEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
     gene            complement(9522..9788)
                     /locus_tag="O0305_000581"
     CDS             complement(9522..9788)
                     /locus_tag="O0305_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCY0273739.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            complement(9788..10045)
                     /locus_tag="O0305_000582"
     CDS             complement(9788..10045)
                     /locus_tag="O0305_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCY0273740.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            10404..10859
                     /locus_tag="O0305_000583"
     CDS             10404..10859
                     /locus_tag="O0305_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCY0273741.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(11027..12604)
                     /locus_tag="O0305_000584"
     CDS             complement(11027..12604)
                     /locus_tag="O0305_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCY0273742.1"
                     /translation="MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            12799..13548
                     /locus_tag="O0305_000585"
     CDS             12799..13548
                     /locus_tag="O0305_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273743.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVIISLAIFRLMHEDAFIPMIRSQIVASA
                     KYKWHILISFTLSVVLFLLLNHFNIFALNVDSFTVFDIYPLIKGYGSISLITIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSIILETLFYYPYINTWWEFIPALILAG
                     SATYLYLKSYRNFMVSYFYQLAVILVLHIFM"
     gene            complement(13772..14965)
                     /locus_tag="O0305_000586"
     CDS             complement(13772..14965)
                     /locus_tag="O0305_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273744.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSFLKKPTPR
                     PASSL"
     gene            complement(14977..15726)
                     /locus_tag="O0305_000587"
     CDS             complement(14977..15726)
                     /locus_tag="O0305_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273745.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(15719..16534)
                     /locus_tag="O0305_000588"
     CDS             complement(15719..16534)
                     /locus_tag="O0305_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273746.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSESQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            complement(16531..17400)
                     /locus_tag="O0305_000589"
     CDS             complement(16531..17400)
                     /locus_tag="O0305_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273747.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(17397..18332)
                     /locus_tag="O0305_000590"
     CDS             complement(17397..18332)
                     /locus_tag="O0305_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273748.1"
                     /translation="MFKFILKRIALMFPLMIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(18345..19943)
                     /gene="cntA"
                     /locus_tag="O0305_000591"
     CDS             complement(18345..19943)
                     /gene="cntA"
                     /locus_tag="O0305_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCY0273749.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(20086..21387)
                     /gene="cntM"
                     /locus_tag="O0305_000592"
     CDS             complement(20086..21387)
                     /gene="cntM"
                     /locus_tag="O0305_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCY0273750.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKAKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMVILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQHLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(21380..22198)
                     /gene="cntL"
                     /locus_tag="O0305_000593"
     CDS             complement(21380..22198)
                     /gene="cntL"
                     /locus_tag="O0305_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCY0273751.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(22209..23030)
                     /gene="cntK"
                     /locus_tag="O0305_000594"
     CDS             complement(22209..23030)
                     /gene="cntK"
                     /locus_tag="O0305_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCY0273752.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(23897..>24090)
                     /locus_tag="O0305_000595"
     CDS             complement(23897..>24090)
                     /locus_tag="O0305_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273753.1"
                     /translation="TVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
BASE COUNT         7671 a         4641 c         3565 g         8213 t
ORIGIN      
        1 tctttggcaa ctaaatagat aactcatatt ttttaaaagc gaatttatta tcgctcaatt
       61 ggttgtttta tagcaaaaaa gtgtcgaaaa tgtgaaatat tattacaaaa gtcgaatttt
      121 tgtaataatg cacacgcatc cattgttaca gacggacaac cgcccatccg ccactcacaa
      181 agtcatcctt atattttaat atcttcatca ttcattgtag acacccttgc cttttgctaa
      241 cattttctac tcccaagcct gtaacggaat tccaacgtca aagtgttgcc catgacagac
      301 acacctaaaa aaacaaccaa cagcatgagc cattggttgt ttcgtgtcat cttgtatgtt
      361 gttgtataac cggtatgctt tactcattaa ttacttatca tgcatccctt taccttctag
      421 tatttggtta atatactttt caacacgctt ttgtcttgtt tcactgcgtt tagcttgtcc
      481 aatatgatat atatattgat gttgtctgcc tggcgttaat ttataaaatg aagactctaa
      541 ctgtggcatt tcttcgaact tagcttctaa ctctttagga ataacatatt cctctgtttt
      601 cttcatttca acttttttac cagctttttc agctttaatt gcttcggcta gataatattt
      661 aatttcttcg gtacgtgctt gaatctctgt taaattttca aatcgtaact gacgagctgc
      721 ttgcaccttt tcagtctgtt gaatgagtgt atgatattta tcctccaaaa tggcaccttt
      781 atgaaataat agtgcaacat aatttttgaa gccatggata agtactacat ttttattatt
      841 caacgtgtaa caaggatgca tccatttata cgcttcctct aattctgttt cattaaagat
      901 taacgctctc aaatatttat attcttcttg ccattgactc tctttattta aaaatgcttc
      961 aacttgttca tttgattgtt tctcactcat agtcatcact tccattttgt ttttcaatac
     1021 acattgtatt atatatccta attttataaa aaactaattt tacaaatgct tttgcgttct
     1081 tacaaaaaat gcatttgact attattataa taagcgtata attgtcgcat attatttttt
     1141 gtatttttgg caataacgaa ggagtattta tgaataaaga caagcaattg cacaacgaca
     1201 aaatcaatct atcccaatta gtcttattag ggttaggctc tttaatagga tctggttggc
     1261 tatttggtgc gtgggaagca tcatcaatag ctggaccagc agcaatcata tcatgggttc
     1321 ttggattcct agtcattgga accattgcct ataactacat tgaaatcggc acaatgtttc
     1381 ctcaatcagg tggcatgagt aactatgccc agtatacaca tggctcatta ttaggcttta
     1441 ttgctgcttg ggcgaattgg gtgtctttgg tgacaataat acctatcgaa gctgtgtcag
     1501 ctgttcaata tatgagttct tggccgtggc attgggcgaa accaatgaga tatttaatgg
     1561 aaaatggctc tattagcaca tacggattgc tagctgtata tctcatcatt gttatttttt
     1621 cattattaaa ctattggtcc gtaaaacttt taacatcatt tacgagttta atttctgtat
     1681 ttaaattagg cgtacccatg ttaaccatca tcatgttgat gctatcagga ttcgacactt
     1741 caaattacgg ccattcggca agcacattta tgccttacgg aagtgcaccg atttttgctg
     1801 caacaacagc atcagggatt attttttcat tcaattcatt ccagacaatt attaatatgg
     1861 gttcagaaat taaaaatcct gaaaaaaata tcgcaagagg catcgctatc tcactgtcaa
     1921 tcagtgcagt gttgtacatc attttacaaa gtacgtttat cacttctatg cctcaatcaa
     1981 tgttacaaca tagtggatgg aatggcatca acttcaattc accatttgct gatttagcta
     2041 tcttattagg aattaattgg ctcgcaattt tactatacat tgaagctttt gtatcaccat
     2101 tcggtactgg cgtgtcattt gtcgccgtta caggtcgagt tttacgagca atggagaaaa
     2161 atggacatat ccctaaattt cttgggaaga tgaatgaaaa atatcatatc ccacgtgtag
     2221 caatcatctt taatgccatc attagtatga ttatggttac attatttaga gattggggta
     2281 cgctagcagc agttatttct actgcaactt tagtagccta tttaactggc ccaacgacag
     2341 tgattgcatt aagaaaaatg ggaccaacaa tgactcgtcc atttagagca aaaattttaa
     2401 aagtaatggc accattatca tttgtattag cttcattagc tatatattgg gcaatgtggc
     2461 caacaacggc tgaagttatt ttaatcatta tacttggatt accaatctac ttcttctatg
     2521 aatatcgtat gaattggcgt aatacaaaga aacaaattgg tggtagctta tggattattg
     2581 tatatttaat cgcgctatca atactgtcat ttataggaag caaagaattt aaaggcttaa
     2641 atatgattca ctatccattt gactttatcg ttattattat tgtggcactt atcttctatt
     2701 acatcggtac aacgagttca tttgaaagcg tctatttccg tcgcgcaaca cgaatcaata
     2761 cgaagatgcg tgagtcacta aataatgaat caaagtcatc tcattaaata ttttaaaata
     2821 cagtgccata tatgatttca acacacacac attaaccaac cattgatttc aacatcttgg
     2881 ttggtttttt attttgaaaa tcggttataa ataactaaca taacaagatg atgatcaggc
     2941 tgggacataa atcaatgttc tatgctctac gaagttatat tggcagtagt tgactgaacg
     3001 aaaatgcgct tgtaacaagc ttttttcgat tctagtcagg ggccccaaca cagagaattt
     3061 cgaaaagaaa ttctacaggc aatgcaagtt ggggtgggac gacgataaag aaatactttt
     3121 tctatagaaa ttagtatttc ttatgcatga gttttactca tgtattcata tttttaagta
     3181 cacattagct gtggctaatg tataagaacc actacataat aaatcatttg tggctcttta
     3241 tcatttctgt cccactcccg tagaagtaca tcatataatg ctgaaaatgg tttgagttaa
     3301 aacagatatc aagctcgtct gattcagtca caaaattgtc ttgttatact tgtcacctat
     3361 catctataga ccgtggtatg attaaattgg ggatgataaa ggaggttaat aaatatgaag
     3421 attaatacta caggtggtca aattcatggt attacacaag atggtttaga tatcttctta
     3481 ggcattcctt atgcagaacc accagttcat gacaatcgct ttaaacattc tacgttaaaa
     3541 acacaatggt cagagccaat tgatgcaact gaaatacaac ccatcccacc gcaaccagac
     3601 aacaaattag aagatttttt ctcctcacaa tctacaactt ttactgaaca tgaagactgt
     3661 ttatatctaa atatttggaa acaacataat gatcagacga agaaacctgt catcatttat
     3721 ttttatggtg gtagttttga aaatggtcat ggtacagccg aactctatca accggcacat
     3781 ttagtacaaa ataacgacat tatcgttatt acatgcaatt atcgtttagg cgcattagga
     3841 tatttagact ggtcatattt taataaagat tttcattcca ataatggcct ttcagatcaa
     3901 atcaatgtca taaaatgggt gcatcaattt attgaatcct tcggtggcga cgctaataac
     3961 attactttaa tgggtcagtc tgcaggcagt atgagcattt tgactttact taaaatacct
     4021 gacattgagc catacttcca taaagtcgtt ctactaagtg gcgcactacg attagacacc
     4081 cttgagagtg cacgcaataa agcacaacat ttccaaaaaa tgatgctcga ttatttagat
     4141 acagatgatg ttacatcatt atcgacaaat gatattctta tgctgatggc gaagctaaaa
     4201 caatctcgag gaccttctaa agggcttgat ttaatatatg cgcctattaa aacagattat
     4261 atacaaaata attatccaac aacgaaacca atttttgcat gttatacaaa agatgaaggc
     4321 gatatttata ttactagtga acagaaaaaa ttatcgccgc aacgctttat cgacattatg
     4381 gaattaaatg atattccttt aaaatacgaa gatgttcaga cggcgaagca acaatcttta
     4441 gcgattacac attgttattt caaacagccg atgaagcaat ttttacaaca actcaatata
     4501 caagattcaa acgcacaact atggcttgct gaatttgcat ggcatgatac ttctagcgca
     4561 cactatcgca gtgcttacca tattctagat atggtatttt ggttcggaaa cttacaaata
     4621 ttagcagcac atcaatatcc aacaactgct caccttaaat ttttaagtcg acaaatgcaa
     4681 aatgatttag ctaattttgc taaaagcggt aaaatgcctt ggccgatgta tcataatgaa
     4741 cgtcgctatt atcgcacata tcaataacgt tatcgcatct aaaaaagcac acctgaattt
     4801 gatagcaggc gtgcttttca tatgagaatt atgatgattt aaatgtttta gcataatgat
     4861 tatttttcaa tgtgaaaaca acgattaatc ctaatgcgcc aatgatggca ctcgtatatg
     4921 tgatagcttg aacagaaaca tgtgtcatga ccaagcctcc aataatgcct ccaacaccaa
     4981 taccagcgtt taaactagac atgttccaac tcattacttg gcttgtgtcg ccttcaacat
     5041 gttgaatcac accgctttgc actgctggat tagtactcca ttgcatgata ttccaaataa
     5101 atagtcctgc taacaataga cctgaaccag gtaagattaa attcataagt aacatcatga
     5161 cgataaaaat agaaacagaa atcattaacc aacgcttact tgtaatttta tcggagaata
     5221 taccacctaa tgatgttcca ataacgccag cgattccatt tactagaagt gctaatgaaa
     5281 cgaatgacat atcatgacca ttagataaaa taagtggatt tataaagacg aatgtcactg
     5341 agtttgcaat caatactaaa aacgtaataa ttaaatattt tgctacttca gcaggtctta
     5401 atattttcga agtaacatga ttttcatgag atggtgtctc atgattcaca gggcctcgtt
     5461 gtatttcctg atccttcggt aaatagatca tcatcaagaa gccaacaata atactcacaa
     5521 taattaagaa tagaaatgta tagcgccaac ctactaaatc gccgataacc gttccaattg
     5581 gtacaccaaa aacattagca ccactaaacc ctgtatagac aagtccaatc attttaccac
     5641 gatttttagg tgctgataac atcgctgtaa tagctaatac ttttacgata attagtgctg
     5701 ctgctgcaga tgagataatt ctaccaacta ctaatattga aaaatttggc gctacagcaa
     5761 taatgccatt accaatgata aatataagta atgtccataa taatacaggc cttgatgaaa
     5821 aacggttcgt taatttaacc agaataggtc cacatatcgc aaatgttaaa gcgtacattg
     5881 tcactaattg accaacgaca gcttctgata catgtaagtc ctgactcatc aagttcataa
     5941 ttcctgcaac catcatttca accattccta cgataaaaat acttaaaatg aatgtaaata
     6001 gtcgcatgat tgacaattta aaactccttt caaacaaaat ttccgatatt catttccctt
     6061 agaataacga tttattaaaa aatgtgtgca acacaatttt atatataacc gttctattat
     6121 ataatatttt gctttaattt tctgtgctta attggaaaat tattgaaatt gtttttcagt
     6181 atttgaaaaa ttaacacaag tatgtcattt aataattatg tataattact tcagtttcaa
     6241 caataactcc aaaaaagcac gcacgcttta tatgtcagaa cagataactg actacaaagt
     6301 atgcgtgcct ttatgaatat tttcgctgcg ctaatgaata gcgatactat atgtgcgcgt
     6361 cacttcattt ccaattaaaa ctaatgccta aatctgatgc agtaaaatct atcatgattc
     6421 actcttaaca tcagtatttc gtgctactaa ttgatgtctt gcattgaaaa attgaccata
     6481 gcttaaatat gtcgcaatca aagcagacat aatggtcgca gttgtatgaa taaacacgac
     6541 taacaattga aatttaatcg cttgtaaagg tggtacgcca ccaataatta agcctgtcat
     6601 cataccagga atcgacacaa gcccatatgt tttaaccgaa tcaatagttg gcactatagc
     6661 taaacgaata ctttcacgta ttgcaccttt agaagccaat ttaggtgtag ctgcaagtga
     6721 taatttagat tcaatattag taccatcttg tacgaatgca cgatctaaat tctggtaagc
     6781 taaattaatt gcaatcaagc cattatttgc aagcatgccg ccgataggta taacttcatt
     6841 tgcggtaaat tgaatggccc ctgtcgcaat agtacctgca agcggtaatg ccgttccaat
     6901 gaagatagct agaaatgata tccaaaacac atgatgcatc actggtgatg ctcgactaat
     6961 tgtattccat gatgcattaa taataatgac caatacacat aaaataagca gccatttatc
     7021 gtttatttta aaaatataat gcagcaaaaa tcccaaaatg attaattgca caactgctcg
     7081 taatgtcgca acaattaaat ctttaataat atgtaaacct tctttatatg aaatgataat
     7141 cggaatgact aaaagcaatg ccgtcaatcc aagtgccgta ttactcatgt tgattcaact
     7201 cctctgtatt agaaatttga ccatcaacaa ttgttatacg cttttgaaag tgtcgcatac
     7261 tttggtcatc gctgtgggta atccacataa ttgccacgcc ttgatctgct aatttaaaaa
     7321 tgatattttc tatcttttct ttattattaa cgtctaatgc actggtcgat tcatctaata
     7381 aaagaatatc cggtgtatac atcagttggc gcgctatagc aattctttgc cgctcaccac
     7441 ccgacatatt ttccacttcc gaacttaatt gataatgtcc caaaccgaca tctttaatta
     7501 attgctttgc acgttttcta tcaaatttat cattacgtgc aagtgatggg aatatcatgt
     7561 tatcttcaat cgtttcacca aacaagtcac tttgctgcat caaataactg attcgttgac
     7621 gcaattcttc cgggtcataa tcattatagg gtttaccttt aaaataaagt tctccactag
     7681 ttggactaaa caaattacat atttgctttt gaaatgtact tttaccacta cctgatggac
     7741 ctataatggc aatactctcg cctttatcta cttttagact gatatgatct agtatgattc
     7801 tatcgctcgc tttatacact aaatctttaa tttctaacaa catttgcacc gctcctcatg
     7861 taatatatca ggtatattat tcccgttttt ttggttttta gtgcaattac atttaagaaa
     7921 aaatcatttt cttgtaatat caaaatttag gcataattga ttttcagcat ttaaattaaa
     7981 aacaaaaaat cctattaaca tggatactgt ctaatgacta agaattaaaa ttagacaatt
     8041 gtttaacaac ctaccatatt aaaaggattc tttattattt atggatttga ttaccacatg
     8101 atatttttat aactttcatc attcaatgaa cggacaattt cagtaacaag acggatagaa
     8161 ttttcataat catctacatt gagcactgaa acattagaat gcatgtatcg cagcgtaaca
     8221 ccgattgtca tcgttggaat accttcattt gcgacatgaa tacttcccgc atctgtacct
     8281 ccacctggtg tcgtatccca ttgtacttcg atgttatgtt ccttagctac atctttaata
     8341 tgctttcgca aaccttggtg agcaatactt gtagcatcca tcataatgac aactggacca
     8401 ccgcctagtt tactatcgct cgtttgacct gacatacctg gggtatcata agcaatacct
     8461 acatcgacag ctatcgccaa gtctggttta atcgtattcg ctgccacttt cgcaccacgc
     8521 aaaccaactt cttcttgcac tgtggcacca ctgtataagt taatgccaat attttcatct
     8581 tttaaacgtt ttaatacctc aatagctaat gcacagccat agcgattatc aaatgctttc
     8641 gcagttaaat atttatcatt tgcaagcact tcaaattcac tatatggcgt aaccatattg
     8701 cctacttcaa cgccagcttc ttcagcttct tccttgctac taacaccaat atctataaac
     8761 atatttttga tttccattgg ctttttacgt tcttcaggcg ttaagacatg tggcggttta
     8821 gaaccgatga tacctctaat ttctttgccc gaatctgttg taatcgttac tttttgagat
     8881 agcatgactt gattccacca tccaccaact ggcgtaaatg aaataaaacc atgtttatca
     8941 atctttgtta ccataaaacc aacttcatcc atatgaccag aaatcataat tgagtattga
     9001 ccattctcag catttttctt tccaaaaatg ccacccaagt tatcttcaat aatttgatca
     9061 ctgacaggct ctatatagtt acgcattgct tctttaactt gcatttcata accagcaatg
     9121 ccgtttacat ctgttaaatg tttaagtaat ttgactgact gattcatgtt attcatcctc
     9181 ctgtatttca acttaaaact attgtatcac taactttcag aaaaacgttc cctaccgctt
     9241 tatcattttc aggcttaaat atttttaaaa ttttgtgagg aatccgctta aaaacctcat
     9301 atttctaaaa tttaatattc cgaaaaagtc acaatttaaa attccttatt ataatacgtt
     9361 attcttttat tgttactaat ttcaatctgt ttagtcttgc gctattataa aattaaaaag
     9421 tgtagagata acaacgcatt aacgtcgcta ttttccctac acttaaatac tagtttcttt
     9481 aattaactta tcattaaagt tattcagata ttgaatattc attaatcata atgtgaccat
     9541 gccgatagta ttaatacttc tttatttcga tcatctacgg tatagacgac cctgtgctga
     9601 tggttaattc ttcttgaata tcgctctaaa tatttaggct ctaatttttc aaaagattgt
     9661 gttattttat acggatcatt ttttaaagtc tcaacaattt ctaaaaatga cttctttaaa
     9721 taagaatgtt ttattttctt taaatctgat ttcgctgaat ttttaatctt aaccgtgtaa
     9781 ttgctcatta aagattatcc caatcaatat catctatatt tgttgtacca ctattatctt
     9841 tttctctttc tcttactttg tccattgtac cagtagattc aagatatatt gtctcttgta
     9901 tacttttcca atcttctaaa cctataatca cagcattatt ttcggcatta ttgccactaa
     9961 tataaattgg ttcgtgatta ttattaacat tttttagtaa ttgataaaaa tcttttctcg
    10021 cttctgtagg gctagtgata atcatataaa ccctcctttg ttttctgtta cgctaattat
    10081 agcgtacgca cttgagtacg tcaatattac caacgagttc atcgtattct taatacctat
    10141 atgctatcgt tcacaaactt ttgttctttt cttccaacat accgcctata atgctcaaaa
    10201 agaaatcagc agtggaacac actacgttcc ctaccgcttt atcattttca ggcttaaata
    10261 tttttaaaat tttgtgagga atccgcttaa aaaaactcat atcttcaaaa aaaatgacaa
    10321 aatttaaatt tttgaataac atcatttttt agtaaatgtt ataatttctt tataatacaa
    10381 atatcgaaag aaggcgactg actttgaaaa aattatgttc attaattgta gtagcattag
    10441 tttgtatcat tgcattatca gcttgtggta aagaacaaac caaaacatat gaaggtgatg
    10501 ttagtggtaa acatgtacta acttctatca catataaaga tgataaagtt ttaaaacagt
    10561 ctacaatcaa cacaattaaa tatgacgatc taggtatgga taaagacgaa gccaaaaagt
    10621 tattcgccaa atctgaaagt attttcaaag accttaaagg cgtaaaatac aaagtagact
    10681 ataaagataa aaaagcaatt gaacacttag acatagatta cacagaagtt gacatgaaaa
    10741 aattaaataa acgtcttggt gtttcgacta aagaaaataa agatattagt tttgaaaaac
    10801 ttgaaaagca attaaagcac agaggtttaa aagaaaaaga taaaatggac gacaaatagt
    10861 ttataactta aaaatgccct cagataagac taaggttaca aaccttaatt catattctga
    10921 gggctttaat atttgaagtt cttgtgtgac cagcatccac tactaatata aaattatttg
    10981 cagtaacgct aaaatccgct gctttcaatt tcccgaaata attaagttaa ctaatgagtt
    11041 ttaatttata atcatgtatc gtttgtaact caccatcgac ttttcgatat acaatatgat
    11101 cagcagtaat ttctgtagga ctggatacgc caacagctgc tgcaatattg aataagcctt
    11161 catgcaaact tgttacatag tttgtgacac gatattgctt ttctccaaca atcaatgctt
    11221 tttctttctt cgcatctgtc gttgcaacac ctacaggaca cgtattcatg tgacattgtt
    11281 gactcattat acaaccgaca ctaatcatca tcccacgtgc gatatttaca aaatctgcac
    11341 ctaaacctag tgcaatcgca attttatctg gtgtcactaa cttaccagat gccgccaatt
    11401 tcactttatc tcgaatacca tatttttcta acatgccaga cacaataggt agagctgtaa
    11461 atagcggtaa gccaacacca tcttgtaatt cttggaatgt tgcaccagta ccaccttcac
    11521 caccatcaat cgtaataaag cttggatact tatctagttc caccatcgta cgtacaagtg
    11581 tttcaatttc tgaaactttg cttactacaa ttttgaatcc tactggtttt tgacctaatt
    11641 gctgcaactg atcgacgaaa cgaatcaaat cttcagcatt atgaataaat tcgtaacggt
    11701 taggtgaatt gattgtttta taaggttcaa catttcggat tttagcaatt tcttcgttta
    11761 ccttttcagc ttccatatga ccaccacgag tcttagcacc ttgtgccaac ttcagctcaa
    11821 atgcgcgtac gttagataac tgtgcaacct ctttaaataa accttcacta aaattacctt
    11881 ctttatcacg aacaccaaat aaaccgggac caatttggaa aatgatatcc ccattacctt
    11941 ttaaatgata ttctgataag ccaccttcac ctgtattcat ccaagtgccc gctttagcta
    12001 gacctttaga taaagctgta atggcatttt ttcctaaagc gccataactc ataccagatt
    12061 gtcctacgat acgttttaaa ataaatggat gttttaaatg ttcacctaat tttattgcat
    12121 ggtcatcact taagtaatac ggatcaatct ttgtcggcac acgatattct tcacgactaa
    12181 ataaacgctc attcgcgatt ttataaatga atgttgataa caatgttgta ttatctactg
    12241 aaatctcatt acgttgcatc ggaaacattg tgttctgtat gtaaaagccg tcttgataat
    12301 ctttagtagt accgaagctg gtcatacgag agttatattt tccagccaaa acgatatttt
    12361 tataatcatt acgtgaaaaa ggtttccctt cattatcccc agaaaataaa tactgacgta
    12421 attccggtcc cattttttct gaaatatatc taatacgtgc tagtaaagga taattcctta
    12481 atacactatg ttgtgattgt cttttatctt taattaacca aataagcccg ataacaataa
    12541 ccgtaagcat gaatcctaca acgataatgt taactataaa ttgcatgact gtaagaaacg
    12601 tcattacaat acctcccccc aaaatttcaa ttcaatattt atgatacacc ttacaaaaca
    12661 aaacacaatg gaagcgcttc attttataaa acaattttat gatatgtttt tcattttaaa
    12721 ttttaatgta taaaacatac aatacaaagt aatatgtgct aaagtatcta tataatacaa
    12781 ctatttaaga ggtatactat gtcaaataca aataaacatt acatagaaga agaatacgct
    12841 accgaacaat cgcgtttttt caaacgtgat attggattta ttttccttta catatttttg
    12901 gttaacatct tgccgatcat tatcgtcttt ttaacttttg ggttagtagc tgtattcact
    12961 caacaagctc cgtggggttt ctcttttcct atttcaatga acattagcta tttactcgca
    13021 gttattattt cactagctat ttttcgtctg atgcatgaag atgcgtttat tccaatgata
    13081 cgttcgcaaa tcgttgctag tgctaaatat aaatggcata ttcttatatc atttacacta
    13141 agtgtcgtac tctttttact tttaaatcat ttcaatattt tcgcactaaa tgtagattcc
    13201 tttactgtct ttgacattta cccgctcatt aaagggtatg gttccatcag tttaataact
    13261 atattttcgt taaccattat attaaatact ttgatttgtc aacttatttt ccgtcatatt
    13321 ttaattcaag aattaagcag attaatacct ttaaagatta caatagtatt gtccattatc
    13381 cttgaaactc tgttctacta tccatacatt aatacctggt gggaattcat tccagcattg
    13441 atactagcag gtagtgcgac ttacttatat ttaaaatcat accgtaattt tatggtgagc
    13501 tatttctatc aattagcagt tatactcgtt ttgcatattt tcatgtaaaa tttttcagag
    13561 tattatccat tatccataca catcaaaaag cacacttaaa agtcagtatc aaaactcact
    13621 tttaagcgtg ctttatttag atatactgtc gttctaaaaa cgagccatat agaatgtttc
    13681 atgtcctagc atgaagcaca tgaatcgcca tgctcaatat accttttaaa atttaaatat
    13741 tcattaactt aatatgcttt aattcacttc attaaagact actcgctgga cgtggtgttg
    13801 gctttttcaa gaaactcata atcattatta caatgactaa cataccacaa attagataca
    13861 tccatttaaa tcctactaaa tcggaaatag gtcccatcaa tgcgccacct aacgatatac
    13921 ctaagtctgc acaggcaata aataaaccta acaacatatt acgacctact tttggtaaga
    13981 cgaagcttaa gtatgatgtt aatgttgggt acaccattgc ttgcgtcatt cctattaata
    14041 tcgcactacc atagaaaata attgcaccta cttgcggacc aaatgccact acaaatgacg
    14101 cgattactaa taatgatagt acagatacca tatatttagg atgccacata ccatctgacg
    14161 gaatgtattt ccttaagtaa aatcttgccg caacaactgc gatggcctgt attgtcaaaa
    14221 agattcccgc attcgcgaat cctaaactca ctgtgtataa cggtacaaat gtactaactg
    14281 caccaaatac aatcgatgca acaatcatga taataccact gttcaacaac tctttatttt
    14341 tgaaaaattg cgcaaaaaca gttacagcgt taaacggcat tttttcaatc ttatctgacg
    14401 tatcgggttc ctgttcagca aaggtcacgc gatagccaaa gaatgttgtt gttaatgcga
    14461 taaagataat gacaattgca aatagtgaaa tattatttgc attccaaata cctacggcaa
    14521 ctaatggtcc gattaagttt ggaatcgttg aaaatagcga gtacaatgat acaccttcag
    14581 aacgatgttc ctctggtaat gcatcaataa tacctagctg taaagacata gaaaagaatg
    14641 ccgtacacac accttgcata acacgtgcta cgaaataacc ttctaagcca gtaaaaccat
    14701 aaataattaa agctatggca ttgataatca aaataattct taatacttta atgggaccga
    14761 cacgagcaat aatttgtcct gcccatggtc gaaataccat tgctgttaac atgtatgccc
    14821 ccataacgat accgataacc gtattcgttg cgcctaaatc atgccctcgt aaaggtataa
    14881 acacgtttaa gattgcattg gcactaaaga acatcaatgt taatatatat aatctcaaaa
    14941 agggccaagc cattgcacct ttcattatct cactccttag aatgatagtt gtttttctat
    15001 taattctctt gtataagcat tgtcactttt atgcaatgcg cttgtcggaa tttgttcttc
    15061 tatttttccg tttttaaaaa taattaattg atcacataaa tacgtggcag cttgaatatc
    15121 atgtgtgata aaaatataac tcaactgacg cgtttcacgt aaatgaatca ataaatctaa
    15181 tatttgtgtt tgaattgaca tgtcgagtga actaatggct tcatcaaaca aaatatattt
    15241 agggttaata catattgcac gcgcaatcgc aacacgttgc gcctctccac ctgataacat
    15301 attaggatat ttatccatgt atgccttaga tagaccgact tcttccaaca atgtaattgc
    15361 ttggacttcc ataacttctt taggttgtcc atcacattga cacatcactt caaataagat
    15421 ttctctaaca gtctgaaatg gatgtaatga tgacgtataa tcttgaaata cagcaccaat
    15481 ttgatgacgt ctcactttct tcttatgcat cggttgatca tttaaggtta cacaaccttt
    15541 atccggtttc tcaataccta atatcatacg actcaacgtc gatttaccgc taccactttc
    15601 tccgataatc gcaatcgtcg caccgattgg acactcaaat gacacacctt tcacgatagg
    15661 tgttcgacga cgcttaaaaa catgtgcgct ttgatatgac ttttcaacat ctttaatttt
    15721 aatcatgtac atcacccctc atcacatgtt taaaatgatc attaatcttc ttcttcgttg
    15781 ataataaata cttcgtataa acatgttctg gatgatgcaa gactgattca cgtgtccctt
    15841 gttcaataag ctgaccattt ttcatcacaa caacacggtc tgcaatcttg ttaataaccg
    15901 ttaaatcatg tgaaatgaaa atcatcgcac agtcaaagtg ttttttaata tctataaatg
    15961 cttccagtac atcatattgt gtaattgtat ctaaagccgt tgtcggctca tcagcaatga
    16021 ttaactttgg tttcaaagct aacgctaaag caatcatcaa tcgctgtaac attcctcctg
    16081 ataacatgta agggtatgat tttaatatac gtttaggatc tttcaaactt aaataatcca
    16141 tatattcaat caatgtcttt tcaatttctt gtgtagacat tgacgtatgt actttcatag
    16201 tctcaaacat ttgtttaccg acagtagttg atgggtcaaa ggcacgacta ccttgttgca
    16261 tgaccatcgc aatgtcttta ccacggtact ttttcaattg cgattcagat aatgacaaca
    16321 ttgatgtacc atcaaagata atttcacctg tcaccccgag tcgttcggga ttcaaaccaa
    16381 taatcgattt acaagtgatt gatttaccac taccactttc tccaataacg cctaaagttt
    16441 cacccttagt taatgtaaaa ttcacatcac tcacgagtgg ttgatctgtc caggtatctg
    16501 taatcgtcaa atgtttaact gttaacaatg tcatgattgc accactcctt ttttcacaga
    16561 acgaagttta tctttagaag agatgcgggg atcaatagca atttgtaaag catcggataa
    16621 gaagttaaat gccatcacta taatcactat ggcaatacct ggcgcaaaca tcatttcagg
    16681 atgtgtaaac atcacttttc tagcttcgtt aagcatcatg ccccactctg cagtaggcgc
    16741 tttgacacct aatcctaaaa atgaaaagcc agatatttgc aagatcattg aacacatcga
    16801 gctactagag atgatagcaa tatctgctaa tgttaatggc ataatatgtt tgtgaataat
    16861 tttcatatca ttcataccga ttgttttagc aaatcttaca tggtcagaag cagtgtactg
    16921 cataacactt gtacgtataa cacgacagaa ccatgcccaa cgcgtcaaaa taaatgccat
    16981 gataatattt tcggcaccca ttccaaacaa tgcaattaat gctaacgtta caacataact
    17041 tgggaatgcc aacataacat cacacgcacg catgattaag gcgtcaacaa acccttggaa
    17101 atatcctgat aagaatccta aaatagatcc aataagtaca gaaacaaata gcgcaacaaa
    17161 gacatataac aaacttggtc taatcgcata aattaaccta gttaaaatat ctctacctaa
    17221 atggtcagta cctagtagat gttgaaaact catgccagca aatttgtttg ctgtatcgat
    17281 atggttagga tcataaaatg tcacaagtgg tgctgctaat cctaaaaaga catataatac
    17341 aataatgcct aaagcaatta ctgcaccttt atcttgtaat aatcgtttta aaattatcat
    17401 cgtgcgccct cccttaatct tggatttaat agcgcattaa tgatatctgc taatgtatta
    17461 aatacaataa ataataccgc tacaattaat acatatgctt gaatgactgg aaaatcgtgt
    17521 tcaagtattg cttttaaact taattgacct agtccaggcc atgcaaagat atactcgata
    17581 acaactagtc cacccattat cattggtata gacatacaaa agattgatac cgcaacttgt
    17641 aaagcattac gcaacacatg caacattaat gtgatagatt tcacaccgct tgctcttaaa
    17701 taaagtacat aatcttcatt taattgttcc accatcgagc gtctaacatt tctaaagtaa
    17761 ataccagcat aggcaatcgt aataacgatc actggcaata tgtaactttc tggacctgtt
    17821 aatccagaag tcggcaatat gtttaacttc actgaaacgt aaataataag tattgaagct
    17881 atccaatatg atggtaatgc agttagaaag aaagccactg aacgtatcgc acgatcagtg
    17941 aactttcctc tttttaatgc actaactaca cctaaaataa ttgatgtaat catcaccata
    18001 acacttgaaa ttattgttaa tttcaatgta ttcataaatg ctggaccaat acgttcagca
    18061 actgggtcac ctgtaatgta gcttgtacca aaattaaatt gcatcgcttc aagtaaccaa
    18121 tttttatatt gaattaataa tggatcattg aaaccgtact tctcattcgt ttctgcaatc
    18181 aactctggtg ttacatttgg cgtcccttgt gcatgtaaaa ttgtcacagc tggattttca
    18241 tttgtaatat acgtcaatag aaatgtcata aaacttacta caatcatcaa tggaaacatg
    18301 agcgcaatac gttttaagat aaatttgaac atctaattgc tcctttattt atactgcatt
    18361 tcattgaatg gtaattcata ctgtgattgt gtgaatgata ctttttctaa atcttttggt
    18421 gcaacaactg tcatactacc gtgtgaaata gggataaaga taccttcatc atcaatttgt
    18481 ttcaaaatgt ttttataagc gtctgaacgc tctttaccgt tttggatttt aaatgcgtca
    18541 tcaatgctgt tgtatatttt atctttgttc tcaatgcctg atgttgcact ttcataacca
    18601 tttttcgctt taaatgctgc aatagtactt tgtggatcgt acaataatcc ccaagtttgg
    18661 ttgaacatta agtcataatc accagaagta cgacgttcag caattttatc tgatgtttcg
    18721 ccattgatgt ttaacttaat acccattttc ttaaattctg cttgtaagta ttctgcttgt
    18781 tctttttgac ttgaagaacc tttgtcatag tacattgcca tttcaaggtt tttaccatct
    18841 ttttgacgaa catcgctgtc tttacctttc ttccaaccag cttcatctaa taatgattct
    18901 gcttttttaa ggtcatactt acgtgttggc atatcgaaat taatgtctgt tacatttttc
    18961 gcaaataatt gagttgctgg tttttcttga ccatctaaaa tttctttggc aattttatct
    19021 ctgtttacca tatgaccaat cgcttgtctg actgttttgt cactcacagc gttatctttt
    19081 ttaccagaat tgacaactaa cattttcgta ttcataggtt gactacgctt aacttgatag
    19141 tcacctgtat ctttcaattg ttttaaagag tctttgtcta agctatctgt acctctatca
    19201 tctgtgaagg caaagttcgt ttcacctttt ttcattgata ggaatgctgt ttcaccagca
    19261 ggcattactt ttgcttgtac tttgttaagt ttagactttt cgccccagta ttgatcattt
    19321 ttgttaaagt ctgcagactc atcttttttg tgttcaccta atttaaatgg accagtacca
    19381 tcgaactttt taacgccatc ttttgttgta ccgtttttaa agtcttttgg agacacaaat
    19441 acatatggac gaggcatcgc taattcagcc aatgcaggtt gatatgcttc tttcaaattc
    19501 aattcaaccg tgtacttatc tttaacttta acattgtcaa ttaatgtcga aatctttaac
    19561 caagaatgca attttttgtt ttcttgaact gcgtcaatat ttttcttaac tgcgtcagca
    19621 tcaaatggcg taccatcatg gaatttaacg tcatctctca aatggaacgt gtatgtcttc
    19681 ccatcttcag acacatccca ctttttagct agtaaaggct taataccatc tttcgtgtta
    19741 cgtacaagcg gctcgtatat catactttca gcagacattg atccaccgta aacatgcgga
    19801 ttcatatcac cgatatcttt aaccgtcgta tacgttaatt gcttgttttc ttttttctcc
    19861 tctaaacctt tattaccgcc acaaccagtt aaaattagcc ctgatgcaag taacattgca
    19921 ctcattttag ttagttttct catttgcttt tcctctttct aaattgataa gttgcattac
    19981 ttaatcaaat cgtaatgatt atttttctca attatttgct tatttttatg taaatgtcaa
    20041 acataattca ataatttttt tctattataa aacttcaaac ccttattatg aaagcgttct
    20101 attgatttcc aaaaattttg tgacgagtgc tttatctcct tcaaatagat ttgtattaaa
    20161 ttgagaagat aagtgttgat cttgatgcat atccttgtac gcctggcaac ttgcttcata
    20221 gcgagttagg aactgatcaa tcattggcgt tttgatacct agcattttcc caatatgctg
    20281 aatcatcgcc gttctgtaat aatcttcact tggcattctt ggaatttgaa caacatcctg
    20341 ttcattttta tagacttgct taaatggtac agctgaaaaa tcaaagtaat gtccgttttc
    20401 gtctggctgt gaaaatggat caatgaggat tgcggtatat cttacataaa gcagatattc
    20461 ttgtaagata tctggcaaga tttcgaaatg ctcaatatca ccttcatcca aagtttcagg
    20521 acgtactgga taattttcct tcaccataaa ttgaagcagg ttgactgacg gcactctaaa
    20581 tgcttgtaaa ataaccatca tttccttcca cattaaacgc atttcacgga ttagtgtcat
    20641 cgttatcggt ccttcaggaa ataacttata cacataaacc ggtacatctg ttccttcgaa
    20701 aatggctttc aatgaaaagt cattcataaa tagtggtggg tgcacataaa gcgaactgtt
    20761 tcgcgtttca gcatgcagtg gcgattcaac gacttctaat tgaatcttca attgctcagc
    20821 taaagcagag attcgttgac acattgttga gtttgaatgt gtcgatccca tgtacaattt
    20881 cttttttaca cctgttgtca acacatgatt aggcgcttct ttatcaacaa tacgtgtatc
    20941 gccaagataa gttgagaatg aaatcacttc gatatcttta ttaaatttag acataaattg
    21001 ttcgacaatc atttgcgaac caaatgtcgg tgatattaaa atgacatgtt tgacactttg
    21061 caaagtttct aacgacaatt gctgtagtgt gtcataataa gcatctgctg tgcatgccat
    21121 gacaaccgtt tcgtattcac ccttaacgtt tttaacatct ttatacaaac gattaatttc
    21181 aaacttacct tccagatgtt gatgcgcctc gttttgtatt ttgacttcaa attgtttcgc
    21241 ttttttatac gcttgatata agcgttttga tttttctgat gttgaggcac gtccaaccat
    21301 atcaatctca taatctgatt ttaaatagca aatattcgct aattggattg cgaccggacc
    21361 agtgcctatc attaataatt tagacatccg taatacctac ctttatagct gcttttttat
    21421 aaagtgctat atcaaaaatt tgctgtggtc tcatatgttt attcacacat tgccacttat
    21481 cttccgctgt ttcttgtgac ggataattaa atattgcttt gatgccatca ccaaagcgca
    21541 ttgcaactac gacattttca tttgttaaat catataattc ttctaaaatg ctgtacttta
    21601 aaggaattgt cgagctgaat atgatatgcg tcacatcttt gatatcttta agttcagata
    21661 ccttttgatc cgtaattgtt atatcttcat ttggtgctaa gacgttaacg attctgcgcc
    21721 ctaggtcaac ggcttgtgga tcaatatcaa taccgataac tgaagcacct gtttcttttg
    21781 ctacttgaat taacgtcatt ggatatgcac ctgaaccaac taacaataat ttgtcattgc
    21841 tagctatact acattgtcca aactcttcat caatacaatg ttctatattg tcaaagtaac
    21901 ctgtggtaga cgcctgtccg tctaatagac gtctcgctct aatgacttcc acttgtttaa
    21961 cacactgcgc tgtaatatat tgcagtgatt ttattaattg tgctttttct tcaacgtcac
    22021 gccaagcatt aaattgttgt tcataaatag gatttaaaat aaattcacta tagtcatcca
    22081 tcaatgtttc taatgcttcg atatattgat cgtcttgtaa tacacgctcg taatgcgctt
    22141 caaacttttc taaatattgt tgtaatatca atttgatttc attattaaag ttattcattg
    22201 taggctcctt attctatata tgcttttcct gtagctacag ttgtaacctg tcctttaatt
    22261 gaagtttgat atcccaattg atgacatcgc tttgatgtca ctaaaatact gccccctggc
    22321 tgatgtactg taaaatcttt gcatgcgtca ttacgttgat aattattaaa aaccccaatt
    22381 gatgctgtac cagaaccaca gctattttcc caaattaaac tttgaatttc tggtatatag
    22441 attaatggct gtaaaaattg acgttgttca tcaaaaagca tcatacctac tgttttatat
    22501 ttgtgactcc attgttgctc acgcacaaac gcttcaacca aatgttgaat ttctgttgtt
    22561 acttgtttaa ctggaatcac ataatgtaca tatgtttcat aaataatttc tattgctttc
    22621 catgaatgat tacccatatt aattgttgtt ggcacaacac gatgggcttg tggcatttga
    22681 acttcatagt attggcaatc atgaattgcg cattgcacta aatccgaaca gccagacacc
    22741 ttcaccttaa actgttggtc tttaagcaaa tgactttcct gcaaatgatg tatatatgac
    22801 atcgtcgcat taccgcaaaa ttcattaccg ctcataacta agtgaaaatc attaccatca
    22861 tcattttgtg ttgattctat aaagcctacc tgttcacagc atacatgtgt tgcggccatc
    22921 aactgattgg cgatagatgc atattcacta gcatcatgtt ttgaatgaac aagtatcgtc
    22981 atattccccg aaggattata cttagaaaat tctataacct gccgattcat acgacactcc
    23041 tttagatgta tttttagagc ctctgtaatt ttaattacta gaggctctta tgcagttgtt
    23101 gcgaagcaaa caactgtatt cctctaaacc tgtaattttt tagaagatga acctaatagc
    23161 tctatctagt aggtaagtca tgctaattcg caaaagcgaa aaccatggct cttatgcagt
    23221 tgttgcgaag caagcaactg tattcctcta aaactgtaat tttttagaag ctgaacctaa
    23281 tagctctatc tattaggtaa gtcatgctaa cccgcaaagt ggaaagcatg acttagtaat
    23341 cggttgttgt atcaaaatgc aaccgtaaac cttatttaaa gttgcttctt gtggctctac
    23401 aagcgaatgc agttgtccgt taactacatt cacttagatg cttttactgt tttcacctca
    23461 actaaaactt caaaaaagtt gcagaaaaca aatcgtaact attacgattt gattatatac
    23521 tatattagtt gagaatcatt gtcaataaag gttgtaaatg aatgcacatt tatattttga
    23581 taatccttcc ataaacttcg tattctcagc acattcaagt ctttttgaaa atttttatca
    23641 ataattgagt agaagttccg ttctagtcag ttcaaaattt tacgtaagtt gtctctgcgt
    23701 attgctttag tgagaataac ccacacttgt ggaaacgtcc ttttgttaat ctacaatgga
    23761 tacccttgtc tttcgctaac atttcctatt accaatcctg taacggattt tcatcgtcaa
    23821 gttgttgccc atacaatacg caccaaaaaa gctttgtaca atttcgtaca aagctttaaa
    23881 aatttaaata aaaatatcat tgttttaatg aactagccat atcagatttt atttcgtttg
    23941 ctaatacttg taattcaggg aagtaattat tagcaaagcc aattattatt ttatttttta
    24001 caaacgtgtt aatctctcga tcttctggaa tcgtattttc tattaaattc aaaagctcac
    24061 tatgacttat gttctctata cttactgtat
//
LOCUS       DAKTIP010000018        23708 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAKTIP010000018 DAKTIP010000000
VERSION     DAKTIP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23708
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.018"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..219
                     /locus_tag="O0305_000596"
     CDS             <1..219
                     /locus_tag="O0305_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCY0273754.1"
                     /translation="YEFYCRLRDELKNSDLMIEHTNKAGASNIVKNPLSIELTKTVQT
                     LNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            209..1900
                     /locus_tag="O0305_000597"
     CDS             209..1900
                     /locus_tag="O0305_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCY0273755.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVKKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            1905..3143
                     /locus_tag="O0305_000598"
     CDS             1905..3143
                     /locus_tag="O0305_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCY0273756.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            3127..3900
                     /locus_tag="O0305_000599"
     CDS             3127..3900
                     /locus_tag="O0305_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCY0273757.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            3912..5075
                     /locus_tag="O0305_000600"
     CDS             3912..5075
                     /locus_tag="O0305_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCY0273758.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVKKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            5144..5422
                     /locus_tag="O0305_000601"
     CDS             5144..5422
                     /locus_tag="O0305_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCY0273759.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            5434..5766
                     /locus_tag="O0305_000602"
     CDS             5434..5766
                     /locus_tag="O0305_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273760.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            5763..6164
                     /locus_tag="O0305_000603"
     CDS             5763..6164
                     /locus_tag="O0305_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273761.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            6165..6560
                     /locus_tag="O0305_000604"
     CDS             6165..6560
                     /locus_tag="O0305_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCY0273762.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            6595..7236
                     /locus_tag="O0305_000605"
     CDS             6595..7236
                     /locus_tag="O0305_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0273763.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            7328..7783
                     /locus_tag="O0305_000606"
     CDS             7328..7783
                     /locus_tag="O0305_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCY0273764.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            7841..8191
                     /locus_tag="O0305_000607"
     CDS             7841..8191
                     /locus_tag="O0305_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273765.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            8233..8391
                     /locus_tag="O0305_000608"
     CDS             8233..8391
                     /locus_tag="O0305_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273766.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            8405..14605
                     /locus_tag="O0305_000609"
     CDS             8405..14605
                     /locus_tag="O0305_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCY0273767.1"
                     /translation="MNEKVEGMTLELKLDHLGVQECMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENSRIMEKVRLNSGQISEDKAKKLLKIETDLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKSKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSGWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRAKWDNISSTAWSNAKSVWKGTSKWFSNSYKSLKDWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKDWLDQLKGNIGKKAGEFGATAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFEYPIWQRFGRYTGGLNFNGGRHYGIDFGMPSGTNVYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHFQLMQGSHPGNDTAKDPEKWLKSLKGSGVRSGSGVNKAASAWAGDIRRAAKRM
                     GVNVTSGDVGNIISLIQHESGGNAGITQSSALRDINVLQGNPAKGLLQYIPQTFRHYA
                     VRGHNNIYSGYDQLLAFFNNRYWRSQFNPRGGWSPSGPRRYANGGLITKHQLAEVGEG
                     DKQEMVIPLTRRKRAIQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKG
                     NKLTDALIQTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            14605..15429
                     /locus_tag="O0305_000610"
     CDS             14605..15429
                     /locus_tag="O0305_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0273768.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            15438..17021
                     /locus_tag="O0305_000611"
     CDS             15438..17021
                     /locus_tag="O0305_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0273769.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            17021..17311
                     /locus_tag="O0305_000612"
     CDS             17021..17311
                     /locus_tag="O0305_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273770.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            17327..19237
                     /locus_tag="O0305_000613"
     CDS             17327..19237
                     /locus_tag="O0305_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273771.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            19237..20703
                     /locus_tag="O0305_000614"
     CDS             19237..20703
                     /locus_tag="O0305_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCY0273772.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSKIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSRISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRDVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            20703..21092
                     /locus_tag="O0305_000615"
     CDS             20703..21092
                     /locus_tag="O0305_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCY0273773.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            21085..21249
                     /locus_tag="O0305_000616"
     CDS             21085..21249
                     /locus_tag="O0305_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCY0273774.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            21295..21594
                     /locus_tag="O0305_000617"
     CDS             21295..21594
                     /locus_tag="O0305_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0273775.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LDRNFEELRRDKEEDEKNKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
     gene            21730..22032
                     /locus_tag="O0305_000618"
     CDS             21730..22032
                     /locus_tag="O0305_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0273776.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDEESVSSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAESKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            22043..23497
                     /locus_tag="O0305_000619"
     CDS             22043..23497
                     /locus_tag="O0305_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0273777.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMLATGE
                     RLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNK
                     AKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGVVASEVKAPVKNEKNPPVPAGYT
                     LDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLPNNTTITYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDIAGNRISSFGKFSAV"
BASE COUNT         9056 a         3227 c         4793 g         6632 t
ORIGIN      
        1 tatgaatttt attgtcggtt aagagatgaa cttaaaaata gtgatttgat gatagagcat
       61 acaaacaagg ctggtgcgag caatattgtt aagaatccat taagcataga actgacaaaa
      121 acagttcaaa cactaaataa cttactcaag tctatgggtt taactgcagc acaaagaaaa
      181 aagatagttc aagaagaagg tggattcggt gactattaaa gttttaaatg aaccttcacc
      241 aaaactatta acaacatggt atgcagagca agtcactcaa gggaaaataa aaacaagcaa
      301 atatgttaaa aaagaatgtg agagacacct tagatatcta gaaaatggag gtaaatgggt
      361 atttgatgaa gaattagcgc accgtcctat tcgattcata gaaaagtttt gtaaaccttc
      421 caaaggatct aaacgtcaac ttgtattaca accatggcaa cattttatta ttggcagttt
      481 gtttggttgg gttcataaag aaacaaaact gcgcaggttt aaagaagctt tgatatttat
      541 ggggcgaaaa aatggtaaaa caactactat atctggtgtt gctaactatg ctgtttctca
      601 agatggagaa aacggcgctg aaatccatct tttagcaaac gtaatgaaac aagctagaat
      661 attattcgat gaatctaagg cgatgataaa agctagccca aagcttgata aaaatttcag
      721 aacattaaga gatgaaatcc attatgacgc aacgatatca aaaattatgc cccaagcatc
      781 agatagcgat aagttagatg gattgaatac acacatgggg atttttgatg aaattcatga
      841 atttaaagac tataaattga tttcagttat aaaaaactca agagctgcaa ggttacaacc
      901 tcttctcatc tacattacga cagcagggta tcaattagat ggtccacttg ttgatatggt
      961 agaagcggga agagacacct tagatcaaat catagaagac gaaagaactt tttattattt
     1021 agcatctttg gatgatgacg atgatattaa tgattcgtcg aactggataa aagcaaatcc
     1081 caacttaggt gtctctataa atttagatga gatgaaagaa gagtgggaaa aagctaagag
     1141 aacaccagct gaacgtggag attttataac caaaaggttt aatatctttg ctaataatga
     1201 cgagatgagt tttattgatt acccaacact ccaaaaaaat aatgaaattg tttctttaga
     1261 agagctggaa ggcagaccat gcacgattgg ttatgattta tcagaaacag aggactttac
     1321 agccgcgtgt gctacttttg cgttagataa tggtaaagtt gcagttttat cgcattcatg
     1381 gattcctaag cacaaagttg aatattctaa cgaaaaaata ccctatagag aatgggaaga
     1441 agatggctta ttaacagtgc aagataagcc ttatattgac taccaagatg ttttaaattg
     1501 gataattaag atgaatgagc attatgtagt agaaaaaatt acttatgata gagcgaacgc
     1561 attcaaacta aatcaagagt taaaaaatta cgggtttgaa acggaagaaa caagacaagg
     1621 agctttgacc ttgagccctg cattgaagga tttaaaagaa atgtttttag atgggaaaat
     1681 aatatttaat aataatcctt taatgaaatg gtatatcaat aatgttcagt tgaaactaga
     1741 cagaaacgga aactggttgc cgtctaagca aagcagatat cgtaaaatag atggctttgc
     1801 agcattttta aacacatata cagatattat gaataaagtt gtttctgaca agggtgaagg
     1861 aaacatagaa tttattagta ttaaagatat aatgcgttaa ggaggtgaat gttatcgcaa
     1921 aagagaatat tgtcacacgc ataaagaaaa aattgataga caattggatt gatcagtcag
     1981 cttctaagct ttatgacttt agcccatgga aaaataaatc tttttggggt gtaatcaata
     2041 atacgcttga aactaatgaa acgatatttt cagctattac aaagttatct aattcgatgg
     2101 ctagtttgcc cttgaaaatg tatgaagatt ataaagtagt taatacagaa gtatctgatt
     2161 tacttacagt gtcaccgaat aattctctga gcagttttga ttttattaat caaattgaaa
     2221 caatcagaaa tgaaaaaggt aatgcatatg tgctaattga acgagacatc tatcatcaac
     2281 catcaaagct tttcttatta aatccagatg ttgttgaaat gttaattgaa aaccaatcac
     2341 gtgaacttta ttattccatt catgctgcaa ctggaaataa attgattgtt cataatatgg
     2401 acatgttgca ttttaaacac atcgtggcat ctaatatggt gcaaggcatt agtccgattg
     2461 atgtgttgaa gaatacaact gattttgata atgcagtaag aacctttaat cttacagaaa
     2521 tgcaaaaacc tgattctttc atgcttaaat atggttccaa tgtaggtaaa gaaaaaaggc
     2581 agcaagtgtt agaagatttc aaacagtact atgaagaaaa cggtggaata ttattccaag
     2641 agcctggtgt tgaaatcgaa ccgttaccta aaaaatatgt ctctgaagat atagtggcaa
     2701 gcgagaattt aacaagagaa agagtagcta acgtttttca attgccctca gtattcttaa
     2761 atgcaagatc aaatacaaat ttcgcgaaaa atgaagagtt aaacagattt tacttgcagc
     2821 ataccttatt gccaatcgtc aaacagtatg aagaagaatt taatcggaaa ctacttacta
     2881 aaacagacag agaaaaaaat aggtatttta aatttaacgt taaatcttat ttaagggctg
     2941 atagtgcaac acaagcagaa gtgtacttta aagcagttcg tagtggttac tacactataa
     3001 atgacattag agagtgggaa gatttaccac cagttgaagg tggagataag ccgctaataa
     3061 gcggtgattt atacccaatt gacacgccac ttgaattaag aaaatctttg aaaggtggtg
     3121 ataaaaatgt caatgaaagc taagtatttt caaatgaaaa gaaaatcaaa aagtaaaggt
     3181 gaaatattta tttatggtga tattgtaagt gataaatggt ttgaaagtga tgtaactgct
     3241 acagatttca aaaataaact agatgaacta ggagacatca gtgaaataga tgttcatata
     3301 aattcatctg gaggcagtgt atttgaaggg catgcaatat acaatatgct aaaaatgcat
     3361 cctgcaaaaa ttaatatcta tgtcgatgcc ttagcggcat caattgctag tgttatcgct
     3421 atgagtggtg acactatttt tatgcacaaa aatagttttt taatgattca taattcatgg
     3481 gttatgactg taggtaatgc agaagaatta agaaagacag cggatttact tgaaaaaaca
     3541 gatgctgtta gtaattcagc ttatttagat aaagcaaaag atttagatca agaacactta
     3601 aaacagatgt tagatgcaga aacttggctt actgcagaag aagccttgtc tttcggcttg
     3661 atagatgaaa ttttaggagc taatgaaata gctgctagta tctctaaaga gcaatataag
     3721 cgtttcgaga acgtcccaga agatttaaag aaagatgtag acaaaatcac taaaattgat
     3781 gatgtagata catctgaatt ggttgaaaca cctaaagaaa gtatgtcact agaagaaaaa
     3841 gaaaaaagag aaaaaattaa acgcgaatgc gaaattttaa aaatgacaat gaattattag
     3901 gaggaaatga aatgccgaca ttatatgaat taaaacaatc cttaggtatg attggacaac
     3961 aattaaaaaa taaaaatgat gaattgagtc agaaagcaac agatccaaat attgatatgg
     4021 aagacatcaa acaactagaa acagaaaaag caggtttaca acaaagattt aacattgttg
     4081 aaagacaagt gcaagacatt gaagagaaag aaaaagcgaa agttaaagat aaaggagaag
     4141 cttatcaatc tttaagtgat aatgagaaga tggttaaagc taaggcagag ttttatcgtc
     4201 acgcgatttt accaaatgaa tttgaaaaac cttcaatgga ggcacaacgt ttattacacg
     4261 ctttaccaac aggaaatgat tcaggtggag ataagctctt accaaaaaca ctttctaaag
     4321 aaattgtttc agaaccattt gctaaaaacc aattacgtga aaaagctcgt ctaactaaca
     4381 ttaaaggttt agagattcca agagtttcat acactttaga cgatgatgat ttcattacag
     4441 acgtagaaac agcaaaagaa ttaaaagcaa aaggtgatac agtcaagttc actactaata
     4501 aattcaaagt atttgctgca atttcagata ctgtaattca tggatcagat gtagatttag
     4561 taaactgggt tgaaaacgca ctacaatcag gattagcagc taaagagcgt aaagatgcct
     4621 tagcagtaag tcctaaatct ggattagaac acatgtcatt ttataatgga tctgttaaag
     4681 aagttgaggg agcagacatg tatgatgcta ttattaacgc tttagcagat ttacatgaag
     4741 attatcgtga taacgcaaca atttatatgc gatatgcaga ttatgtcaaa attattagtg
     4801 ttctttcaaa tggaacaaca aatttctttg acacaccagc agaaaaagta tttggcaaac
     4861 cagtagtatt tacagatgca gcagttaaac ctattgtggg agatttcaat tattttggaa
     4921 ttaactatga tggaacaact tatgacactg ataaagatgt taaaaaaggc gaatatttgt
     4981 ttgtattaac agcatggtat gatcagcaac gtacattaga cagtgcattc agaattgcaa
     5041 aagcaaaaga aaatacaggt ccattaccca gctaagcccc aaaaggttaa tgtaacagct
     5101 aaggctaaat cagctgtaat atcagccgaa taggggtgat gaaatgagtt tagaagaaat
     5161 taaattgtgg ttgagaattg actataattt cgaaaatgat ttaattgaag gtctcattca
     5221 atcggctaag tctgaattac tattaagtgg ggttccagat tatgacaaag atgacttgga
     5281 atacccgctt ttttgtacag cgattaaata tatcattgca agagattatg aaagtcgtgg
     5341 atactcaaat gaccaatcta gaagcaaggt gtttaatgaa aaaggattgc aaaaaatgat
     5401 tttgaaatta aaaaagtggt aggtgatttt taaatggaat ttaatgaatt taaagatcgc
     5461 gcgtattttt ttcaatatat aaacaaagga ccatatccag atgaagagga aaaaatgaaa
     5521 ttgtatagtt gcttttgtaa aatttataat ccttctatga aagatagaga aattttaaaa
     5581 gcgactgaat caaaatcagg attaaccata attgtcaggt cttctaaaac tgaatatcta
     5641 ccacaaacaa atcacttagt taaaattgac agtgcattat attccgataa attattcaac
     5701 attgtagaaa taagaattga tacaccagat attggctata atacagtggt tttatcagaa
     5761 aaatgagtgt agaaattaaa gggatacctg aagtgttgaa taaattagaa tcggtatacg
     5821 gtaaacaagc aatgcaggct aagagtgata aagctttaaa tgaagcatct gaatttttta
     5881 taaaggcttt aaagaaagag ttcgagagct ttaaagatac gggtgccagt atagaagaaa
     5941 tgactaaatc taagccttat acaaaagttg gcagtcaaga aagggctgtt ttaattgaat
     6001 gggtaggccc tatgaatcgc aaaaacatta ttcacttgaa tgaacatggt tatacaagag
     6061 atggaaaaaa atatacacca agaggttttg gagttattgc aaaaacatta gctgctagcg
     6121 aacgtaagta tagagaaatt ataaaaaagg agttggccag ataaatgaat atattaaaca
     6181 ccataaaagg aattttatta tctgatgcag agctcaaaac acatataaat tctagaatat
     6241 actattacaa agtcactgaa aacgctgaaa cttccaaacc ctttgttgtt attacacctg
     6301 tttatgattt gccttcagac tttatgtctg ataaatatct cagtgaagaa tacttaattc
     6361 aaatagatgt agaatcttca aataatcaga aaacaattga tataacaaaa cgaataagat
     6421 acctgttata tcaacaaaat ttaattcaag catctagtca gttagatgct tattttgaag
     6481 aaactaaacg ttatgtgatg tcgagacgat atcaaggcat acccaaaaat atatattata
     6541 aaaatcagcg catcgaatag gtgtgctttt taatttttaa ggaggaaata agcaatggca
     6601 gaaggacaag gttcttataa agtaggtttt aaaagattat acgttggagt ttttaaccca
     6661 gaagcaacaa aagtagttaa acgcatgaca tgggaagatg aaaaaggtgg tacagttgac
     6721 ctaaatatca caggtttagc accagattta gtagatatgt ttgcatctaa caaacgtgta
     6781 tggatgaaaa aacaaggtac taatgaagtt aagtctgaca tgagtatttt caatattcca
     6841 agtgatgatt taaacacagt tattggacgt actaaagata aaaatggtac atcttgggta
     6901 ggagagaata caagagcacc gtatgtaaca gtaattggcg aatcggaaga tggtttaaca
     6961 ggtcagccgg tatatgtagc cttacttaaa ggtactttta gtttagattc aattgaattt
     7021 aaaacacgag gtgaaaaagc agaagcccca gaacctacaa aattaacagg tgactggatg
     7081 aatagaaaag ttgatgttga tggaacgtca caaggtattg tatacggtta tcatgaaggt
     7141 aaagaaggag aagcagaatt cttcaaaaaa gtattcgttg gatacacgga cagtgaagat
     7201 cattcagagg attctgcagg ttcgttaccc agctaatccc caaaatgttg aagtagcagt
     7261 taattcaaaa tctgcaacag tttcagcaga ataggggctt tcaaaataaa tcaaaggaga
     7321 ataatttatg actaaaactt taaaggttta taaaggagac gacgtcgtag cttctgaaca
     7381 aggtgaaggc aaagtgtcag taactttatc taatttagaa gcggatacaa cttatccaaa
     7441 aggtacttac caagtggcat gggaagaaaa tggtaaagaa tctagtaaag ttgatgtacc
     7501 tcaattcaaa accaatccaa ttctagtctc aggcgtatca tttacaccag aaactaaatc
     7561 aattatggta aataccgatg acaatgttga gccaaacatt gcaccaagca cagcaacgaa
     7621 taaaatattg aaatatacaa gtgaacatcc agaatttgtt actgtagatg aaaatacagg
     7681 agcaattcac ggtgtagctg aaggtacttc agtaatcact gctatgtcta ctgatggaag
     7741 cgataagtca ggacaaattt cagtgacagt aacaaacgga tagggattta aggcgcagta
     7801 tatctgcgtc ttttttattt gaataaaagg agctaataca atgattaaat ttgaaattaa
     7861 agatcgtaaa acaggaaaaa cagagagcta tacaaaagaa gatgtaacaa tgggcgaagc
     7921 agaaaaatgc tatgagtatt tagaattagt aaatcaagag aataaaaaag aagcacctaa
     7981 cgcaacaaaa atgagacaaa aagagcgaca gttattagta gatttattta aagatgaagg
     8041 attgactgaa gaagatgttc tgaacaagat gagtactaaa acttatacaa aagccttaca
     8101 agatatattt cgagaaatca atggtgaaga tgaagaagat tcagaaactg aaccagaaga
     8161 gatgggaaag acagaagaac aatctcaata aaagacattt tatcgaacat taagaaaata
     8221 caacgtttct gtatggagca gtatgggtgg acattaactg aagtcagaaa acagccgtat
     8281 gtaaaacttt tagaaatact taatgaagag aataaagaag agactgaaga aaaacaaagt
     8341 gaacaaaaag tcattacagg tacggattta agaaaacttt ttggaagcta gaaaggaggt
     8401 taatatgaat gaaaaagtag aaggcatgac cttggagctg aaattagacc atttaggtgt
     8461 ccaagaatgc atgaaaggtt taaagcgaca attaggtgtt gttaatagtg aaatgaaagc
     8521 taatctgtca gcatttgata agtctgaaaa atcaatggaa aaatatcagg cgagaattaa
     8581 ggggttaaat gataggctta aagttcaaaa aaagatgtat tctcaagtag aagatgagct
     8641 taaacaagtt aacgctaatt accaaaaagc taaatccagt gtaaaagatg ttgagaaagc
     8701 atatttaaag ttagtagaag ccaataaaaa agaaaaatta gctcttgata aatctaaaga
     8761 agccttaaaa tcatcgaata cagaacttaa aaaagctgaa aatcaatata aacgtacaaa
     8821 tcaacgtaaa caagatgcgt atcaaaaact taaacagttg agagatgcag aacaaaagct
     8881 taagaatagt aaccaagcta ctactgcaca actaaaaaga gcaagtgacg cagtacagaa
     8941 gcagtccgct aagcataaag cacttgttga acaatataaa caagaaggca atcaagttca
     9001 aaaactaaaa gtgcaaaatg acaatctttc aaaatcaaat gataaaattg aaagttctta
     9061 cgctaaaact aatactaaat taaagcaaac agaaaaagaa tttaatgatt taaacaatac
     9121 tattaagaat catagcgcta atgtcgcaaa agctgaaaca gctgttaata aagaaaaagc
     9181 tgctttaaat aatttggagc gttcaataga taaagcttca tccgaaatga agacttttaa
     9241 caaagaacaa atgatagctc aaagtcattt cggtaaactt gcaagtcaag cggatgtcat
     9301 gtcaaagaaa tttagttcta ttggagacaa aatgacttcc ctgggacgta caatgacgat
     9361 gggcgtatct acaccaatta ctttagggtt aggtgcagca ttaaaaacaa gtgcagactt
     9421 tgaaggccaa atgtctcgag ttggagcgat tgcgcaagca agcagtaaag acttgaaaag
     9481 catgtctaat caagcagttg acttaggagc taaaaccagt aaaagtgcta acgaagttgc
     9541 taaaggtatg gaagaattgg cagctttagg ctttaatgcc aaacaaacaa tggaggctat
     9601 gccaggtgtt atcagtgcag cagaagcaag tggtgcagaa atggctacaa ctgcaactgt
     9661 aatggcttca gcgattaact ctttcggttt aaaagcatct gatgcaaatc atgttgctga
     9721 tttacttgcg agatcagcaa atgatagtgc tgcagatatt cagtacatgg gagatgcatt
     9781 gaagtatgct ggtactcctg caaaagcatt aggagtttca atagaggaca cttccgcagc
     9841 aattgaagtt ttatctaact caggtttaga gggttctcaa gcaggtactg ccctaagagc
     9901 ttcatttatc aggctagcta atccaagtaa aaatacagct aaggaaatga aaaaattagg
     9961 tattcatttg tctgatgcta aaggtcaatt tgttggcatg ggtgaattga ttagacagtt
    10021 ccaagataat atgaaaggca tgacgagaga acaaaaacta gctacagtgg ctacaatagt
    10081 tggtactgaa gcagcaagtg gatttttagc cttgattgaa gcgggaccag ataaaattaa
    10141 tagctatagt aaatccttaa agaattccaa tggcgaaagt aaaaaagcag cagatttgat
    10201 gaaagataat ctcaaaggcg ctctggaaca attaggtggc gcttttgaat cattagcaat
    10261 cgaagtcggt aaagatttaa cgcctatgat tagagcagga gcggaaggtt taacaaaatt
    10321 agttgatgga tttacacatc tccctggttg ggttagaaaa gcttcagtag gattagcact
    10381 ttttggtgca gcaattggac ctgcagttct tgctggaggg ttattaatac gtacagttgg
    10441 aagtgctgct aaaggatatg cgtcattaaa tagacgtatt gctgaaaata caatcctttc
    10501 aaatactaat tcaaaagcaa tgaaatcttt aggtcttcaa acattatttc ttggttctac
    10561 aacaggaaaa acgtcaaaag gctttaaagg gttagccgga gctatgatgt ttaatttaaa
    10621 acctataaat gttttgaaaa attctgcaaa gctagcaatt ttaccgttca aacttttgaa
    10681 aaacggttta ggattagctg caaaatcttt atttgcagta agtggaggcg caagatttgc
    10741 gggtgtagcc ttaaggtttt taacaggacc tataggtgct acaataactg ctattacaat
    10801 tgcgtataaa gtttttaaaa ccgcatatga tcgtgtggaa tggttcagaa acggtattaa
    10861 cggtttagga gaaactataa agttttttgg tggtaaaatt attggcggcg ctgttagaaa
    10921 gctaggagag tttaaaaact atcttggaag tatcggcaaa agcttcaaag aaaagttttc
    10981 aaaagatatg aaagatggtt ataaatcatt aagcgacgat gaccttctca aagtaggagt
    11041 caacaagttt aaaggattta tgcaaaccat gggcacagct tctaaaaaag cgtctgatac
    11101 tgtaaaagtg ttagggaaag gtgtttcaaa agaaacagaa aaagctttag aaaaatatgt
    11161 gcattattct gaagaaaata gcagaatcat ggaaaaagta cgtttaaact cgggtcagat
    11221 atcagaagac aaagcaaaaa aacttttgaa aattgaaacg gatttatcta ataaccttat
    11281 agctgaaata gaaaaaagaa ataaaaagga actcgaaaaa actcaagaac ttattgataa
    11341 gtatagtgca ttcgatgaac aagaaaagca aaacatttta actcgaacta aagaaaaaaa
    11401 tgacttgcga attaaaaaag agcaagaact caatcagaaa atcaaagaat tgaaagaaaa
    11461 agctttgagt gatggtcaga tttcagaaaa tgaaagaaaa gaaattgaaa agcttgaaaa
    11521 tcaaagacgt gatatcactg ttaaagaatt gagtaagact gaaaaagagc aagagcgtat
    11581 tttagtaaga atgcaaagaa acagaaatgc ttattcaata gacgaagcga gcaaagcaat
    11641 taaagaagca gaaaaagcaa gaaaagcaag aaaaaaagaa gtggacaagc aatatgaaga
    11701 tgatgtcatt gctataaaaa ataacgtcaa cctttctaag tctgaaaaag ataaattgtt
    11761 agctattgct gatcaaagac ataaagatga agtaagaaaa gcaaaatcta aaaaagatgc
    11821 tgtagtagat gttgttaaaa agcaaaataa agatattgat aaagaaatgg atttatccag
    11881 tggacgtgta tataaaaata ctgaaaagtg gtggaatggc cttaaaagtt ggtggtctaa
    11941 ctttagagaa gaccaaaaga aaaaaagcga taaatacgct aaagaacaag aagaaacagc
    12001 tcgtagaaac agagaaaata taaagaaatg gtttggaaat gcttgggacg gcgtaaaaag
    12061 taaaactggc gaagccttta gtaaaatggg cagaaatgct aatcattttg gcggcgaaat
    12121 gaaaaaaatg tggagcggaa tcaaagggat tccaagcaaa ttaagttcag gttggagctc
    12181 agccaaaagt tctgtaggat accacactaa ggctatagct aatagtactg gtaaatggtt
    12241 tggaaaagct tggcaatctg ttaaatcgac aacaggaagt atttacaatc aaactaagca
    12301 aaagtattca gatgcttcag ataaagcttg ggcgcattca aaatctattt ggagaggcac
    12361 atcaaaatgg tttagcaatg catataaaag tgcaaagggc tggctaacgg atatggctaa
    12421 taaatctcgc gcgaaatggg ataatatttc tagtacagct tggtcgaatg caaaatccgt
    12481 ttggaaagga acatcgaaat ggtttagtaa ctcatacaaa tctttaaaag attggactgg
    12541 ggatatgtat tcaagagccc acgatcgttt tgatgcaatt tcaagttcgg catggtctaa
    12601 cgctaaatca gtatttaatg gttttagaaa atggctatcc aaaacatatg attggattag
    12661 agatattggt aaagacatgg gaagagctgc ggctgattta ggtaaaaatg ttgctaataa
    12721 agctattggc ggtttgaata gcatgattgg cggtattaat aaaatatcta aagccattac
    12781 tgataaaaat ctcatcaagc caatacctac attgtctact ggtactttag caggaaaggg
    12841 tgtagctacc gataattcgg gagcattaac gcaaccgaca tttgctgtat taaatgatag
    12901 aggttctgga aacgccccag gtggtggagt tcaagaagta attcacaggg ctgacggaac
    12961 attccatgca ccccaaggac gagatgtggt tgttccacta ggagttgggg atagcgtaat
    13021 aaatgctaat gacactctga agttacagcg tatgggtgtt ttaccaaagt ttcatggagg
    13081 tacgaaaaag aaagattggc tagaccaact taaaggtaat ataggtaaaa aagcaggaga
    13141 atttggagct acagctaaaa acacagcgca taatatcaaa aaaggtgcag aagaaatggt
    13201 tgaagcagca ggcgataaaa tcaaagatgg tgcatcttgg ttaggcgata aaatcggcga
    13261 tgtgtgggat tacgtacaac atccagggaa actagtaaat aaagtaatgt caggtttaaa
    13321 tattaatttt ggaggcggag ctaacgctac agtaaaaata gctaaaggcg catactcatt
    13381 gctcaaaaag aaattagtag acaaagtaaa atcgtggttt gaagatttcg gtggtggagg
    13441 cgatggaagc tatctatttg aatatccaat ctggcaaaga tttggacgct acacaggtgg
    13501 acttaacttt aatggcggtc gtcactatgg tatagacttt ggtatgcctt ctggaacaaa
    13561 cgtttatgcc gttaaaggtg gtatagcaga taaggtatgg actgattacg gtggcggtaa
    13621 ttctatacaa attaagactg gtgctaatga atggaactgg tatatgcatt tatctaagca
    13681 attagcaaga caaggccaac gtattaaagc tggtcaactg atagggaaat caggtgctac
    13741 aggtaatttc gttagaggag cacacttaca tttccaattg atgcaagggt cacatccagg
    13801 gaatgataca gctaaagatc cagaaaaatg gttgaagtca cttaaaggta gtggcgttcg
    13861 aagtggttca ggtgttaata aggctgcatc tgcttgggca ggcgatatac gtcgtgcagc
    13921 aaaacgaatg ggtgttaatg ttacttcggg tgatgtagga aatattatta gcttgattca
    13981 acacgaatca ggaggaaatg caggtataac tcaatctagt gcgcttagag acatcaacgt
    14041 tttacagggc aatccagcaa aaggattgct tcaatatatc ccacaaacat ttagacatta
    14101 tgctgttaga ggtcacaaca atatatatag tggttacgat cagttattag cgttctttaa
    14161 caacagatat tggcgctcac agtttaaccc aagaggtggt tggtctccaa gtggtccaag
    14221 aagatatgcg aatggtggtt tgattacaaa gcatcaactt gctgaagtgg gtgaaggaga
    14281 taaacaggag atggttatcc ctttaactag acgtaaacga gcaattcaat taactgaaca
    14341 ggttatgcgc atcatcggta tggatggcaa gccaaataac atcactgtaa ataatgatac
    14401 ttcaacagtt gaaaaattgt tgaaacaaat tgttatgtta agtgataaag gaaataaatt
    14461 aacagatgca ttgattcaaa ctgtttcttc tcaggataat aacttaggtt ctaatgatgc
    14521 aattagaggt ttagaaaaaa tattgtcaaa acaaagtggg catagagcaa atgcaaataa
    14581 ttatatggga ggtttgacta attaatgcaa tcttttgtaa aaatcataga tggttacaag
    14641 gaagaagtaa taacagattt taatcagctt atatttttag atgcaagggc tgaaagtcca
    14701 aacaccaatg ataacagtgt aactattaac ggagtagatg gtattttacc gggcgcaatt
    14761 agttttgcgc ctttttcatt agtattaagg tttggctatg atggtataga tgttatagat
    14821 ttaaatttat ttgagcattg gtttagatct gtgtttaatc gcagacatcc ttattatgtt
    14881 attacttctc aaatgcctgg tgttaaatat gcagtgaata cagctaatgt tacatctaat
    14941 ttaaaagatg gttcttcaac tgaaattgaa gtaagtttaa atgtttataa agggtattct
    15001 gaatcagtta attggaccga tagcgagttc ttattcgact ctaattggat gtttgaaaat
    15061 ggaattcctc ttgatttcac acctaaatat actcatacat caaatcaatt tactatttgg
    15121 aacggttcta ctgatacgat aaatccacga ttcaagcacg atttgaaaat attaattaat
    15181 ttaaatgcga gtggaggatt tgaactggtt aactatacaa caggtgatat ttttaagtac
    15241 aacaaaagta tagataaaaa cactgatttt gttttagatg gtgtgtatgc atatcgagat
    15301 ataaatagag tgggaattga tacaaataga ggcattataa cattagcgcc aggtaaaaat
    15361 gaatttaaga ttaaaggaga cgtcagtgat attaaaacta catttaagtt tccttttatt
    15421 tataggtagg tgatttaatg gattatcatg atcatttatc agtaatggat tttaatgaat
    15481 tgatttgtga aaatttacta gatgtagatt atggttcttt taaagaatat tatgaactga
    15541 atgaagctag gtacatcacc tttacagttt atagaactac tcataatagt tttgtttttg
    15601 atttattgat ttgtgaaaac ttcataattt atcatggtga aaaatataca attaagcaga
    15661 cagcgccaaa ggttgaaggt gataaagttt ttattgaagt tacggcatat cacataatgt
    15721 atgaatttca aaatcactca gtggaatcaa ataagcttga tgacgacagt agcgaaactg
    15781 gtaaaacgcc agaatactct ttagatgagt acttaagata tggatttgca aatcaaaaaa
    15841 cttcggtcaa aatgacctat aaaataattg gagattttaa gcgaaaagta ccgattgacg
    15901 aattaggtaa caaaaacggc ttagaatact gtaaagaagc ggtagacctg tttggctgta
    15961 taatttaccc aaatgataca gagattggtt tttattctcc tgaaacattt tatcaaagaa
    16021 gcgagaaagt gattcgatat caatataata ctgatactgt atctgcaact gtcagtacat
    16081 tggaattaag aacagctata aaagtttttg gaaaaaagta tacagctgag gaaaagaaaa
    16141 attataatcc tattagaaca actgacatta aatattcaaa tggttttata aaagaaggta
    16201 cttatcgtac cgaaacaatt gggtctaaag ctactattaa ctttgattgc aagtatggta
    16261 atgaaacagt tagatttaca ataaaaaagg gctctcaagg tggaatatat aagttgattt
    16321 tagacggcaa gcaaattaag caaatttctt gttttgctaa gtcggttcag tctgaaacaa
    16381 tagatttaat aaaaaatatt gataaaggca agcacgtttt agaaatgata tttttaggag
    16441 aagaccccaa aaatagaatt gatatatctt caaataaaaa agctaagcct tgtatgtatg
    16501 ttggaactga aaaatcaaca gtcttaaatt taattgctga taattcaggt cgcaatcaat
    16561 acaaagcaat tgtcgactac gtcgcagata gtgcaaagca gtttgggatt cgatatgcta
    16621 atacgcaaac aaatgaagat atcgaaacac aggataagct gttagaattt gcaaaaaagc
    16681 aaataaatga tactcctaag actgaattag atgttaatta tataggttat gaaaaaatag
    16741 agccaagaga tagcgtattt tttgttcatg aattaatggg atataacact gaattaaagg
    16801 ttgttaaact tgataggtca catccatttg taaacgcaat agatgaagtg tctttcagca
    16861 atgaaataaa agatatggta caaattcaac aagcacttaa cagacgagtt attgcacaag
    16921 ataatagata taactatcaa gcaaatcgta taaatcattt atacactagt actttgaatt
    16981 ctcctttcga gacaatggat atagggagtg tattaatata atggcaacag aagaagttaa
    17041 aatcaaagcg ctacttgaaa acgataaaca gtactttcca gctacacact ggaaagctat
    17101 aaatgggata ccttatgcag gcagtagtga tattgatgga ttgcctcaag acggtatcat
    17161 ttcggtagat gataaaaata aattagataa attaaaaata ggcgaagcag gaattattca
    17221 aaatagcatt gtacagaaat ccccaaacgg taaattgtgg aaaataacag ttgacgatag
    17281 tgggaaactt ggtacagtgc tattttatta gaaaggaagg tgcattatgg aaaatttgta
    17341 tttaataaag gatttgggag ctttagcagg tcgagattat agagctaaag aaattcaaaa
    17401 cctgcaaaga atagagcaat ttgcgcttgg cttgacaaca gagtttaagt tgcatcagaa
    17461 agctaaaaca atgcaacact tcgctgagca aatttattat aatggtagat cgcaagcagc
    17521 agtaaacaaa tctttacaaa gtcaaattaa cgcacttgtt gtggcaccac gtaataacag
    17581 tgctaatgag attgttcaag ctcgagttaa tgtaaacggc gaaacctttg acacattaaa
    17641 agaacattta gacgattggg aaaccaaaac tcaaattaat aaagaggaaa ctataagaga
    17701 attaaataag accaaacaag aaattcttga tatcgagtat cgttttgaac ctgataagca
    17761 agagttttta tttgtgacag aacttgcacc tcttacaaat gcagtaatgc aatccttctg
    17821 gtttgataat agaacaggca tagtatacat gacacaagct agaaataatg gctatatgct
    17881 aagtcgttta agacctaatg gtcaatttat agacagctca ttgattgtag gtgggggtca
    17941 tggtacacat aacggttata gatatattga tgatgagtta tggatttata gttttatctt
    18001 aaatggtaat aatgagaata cattagttcg tttcaagtat acgcctaatg tggaaattag
    18061 ctatggcaag tatggtatgc aagatgtatt tacaggacac ccagaaaaac cctacatcac
    18121 ccctgtcata aatgaaaaag aaaataaaat tctatacaga attgagagac ctagaagtca
    18181 gtgggaactt gaaaactcaa tgaattatat agagataaga agtttagacg atgttgataa
    18241 aaatattgat aaagttttgc ataaaatcag tatccctatg agactaacaa acgaaaccca
    18301 accaatgcag ggtgtgactt ttgatgaaaa atacttgtat tggtatacag gagacagtaa
    18361 tccaaataat agaaactatt taacggcttt cgatttagaa acaggagaag aagcgtatca
    18421 ggttaatgct gactatggtg gaacactaga ttcatttcct ggcgaatttg cggaagcaga
    18481 aggtttgcaa atatactatg acaaagatag tggtaaaaaa gctttgatgc taggtgttac
    18541 tgtcggtggt gatggaaata gaacacatcg tattttcatg attgggcaaa gaggtatttt
    18601 agaaatactt cactcaagag gcgttccttt tatcatgagt gacacaggtg gtagagttaa
    18661 acctttacca atgaggcctg ataaacttaa gaatcttggg atgttaacag agccaggtct
    18721 ttactattta tacactgatc atacagttca aatcgatgat ttcccattac caagagaatg
    18781 gcgtgatgca ggttggttct tggaagttaa gccaccacaa actggcggtg atgtaattca
    18841 gatattgacg cgtaatagtt atgcaaggaa tatgatgact tttgaaaggg tgctttctgg
    18901 aagaactgga gacatttcgg actggaatta tgtgcctaaa aatagtggta aatgggagag
    18961 agtaccttca ttcatcacaa aaatgtcaga tattaacata gtaggcatgt cgttttattt
    19021 aactacggat gatacaaaac gttttacaga ttttccaact gaacgtaaag gggtagctgg
    19081 ttggaactta tatgtagaag cttcaaacac aggtggcttt gttcataggc tagttcgtaa
    19141 tagtgttaca gcatctgctg agatactatt gaaaaattat gatagtaaaa caagttcagg
    19201 gccatggact ttacacgaag ggagaattat aagttaatga gtaatttaga gaaatctgta
    19261 gctataaatt tagaaaacac agcgcattat gaaaatattt caaatctaga tataactttt
    19321 agaacaggag agagtgattc ttctgttctt ctttttaata tcattaaaaa taatcaaccg
    19381 ttattactga gtgaagaaaa tatcaaagca cgaatagcga ttcgaggtaa aggagtaatg
    19441 gtagttgctc cactagaaat attagatcca tttaaaggta ttttaaaatt tcaattacct
    19501 aatgatgtaa ttaaaagaga tggaagttat caagctcaag tttcggttgc agaattaggt
    19561 aattcagacg tggtagttgt agagagaact atcacattta acgttgaaaa aagtttgttt
    19621 agcaagattc cctctgaaac aaaactacac tatattgttg agtttcaaga attagaaaaa
    19681 actattatgg atcgcgcgaa agcaatggac gaggctataa aaaatggtga ggattatgcg
    19741 agtctgattg aaaaagctaa agaaaaaggt ctatcagata ttcaaatagc aaaatcttca
    19801 agtattgatg aattaaagca acttgctaat agccgtatat ctgatttgga aaataaagcg
    19861 caagcatatt caagaacatt cgatgagcaa aagcgatata tggatgagaa acatgaagcc
    19921 ttcaagcagt cagtgaatag tggtggttta gtcacaagtg gttctacttc aaattggcaa
    19981 aaagctaaga ttactaaaga tgatggtaag ataatgcaga ttactggatt tgattttaat
    20041 aatccagaac aaagaatagg tgattcaacc caatttattt atgtttcgca agctataaat
    20101 tatccaagag atgttagtac taacggtact gtcgaatatt tagtagtaac ttcagattac
    20161 aagcgtatga cttatcgacc gaacggtaca aataaagtgt ttgttaaaag aaaagaagcg
    20221 ggttcatggt ctgagtggtc agaattagct attaatgatt acaatacacc ttttgaaact
    20281 gttcaaagtg cccaatcaaa agctaatatg gccgaaagta acgctaaatt atacgcagat
    20341 gacaagttta ataaaaggta ttcggttatt tttgatggaa cagcaaatgg tgtgggctct
    20401 acattgtact taaatgagag tttagaccaa tttattttat taatttttta tgggactttt
    20461 ccaggtggtg actttacaga gtttggcagt ccttttggag gaggaaagat ttcattgaat
    20521 ccctcaaatc ttccagatgg tgatggaaat ggtggaggtg tttatgagtt tggattaact
    20581 aaatctagtc gtacatcttt aactatatca aacgatgtct atttcgactt aggaagtcaa
    20641 agaggctctg gtgcgaacgc aaatagaggg acaattaaca aaattatagg agtgagaaaa
    20701 taatgcaaat attagttaac aagcgtaatg agataatttc atacgctatc attggcggct
    20761 ttgaagaagg tattgatatt gaaaatttac cagaaaattt ctctcaagtt tttagaccta
    20821 aagcctttaa atattcaaat ggggaaatag tttttaacga agattattca gaagaaaaag
    20881 atgacttgca tcaacagatt gacagtgaag aacaaaacac agtcgcttct gatgacatct
    20941 tacgaaaaat ggttgctagt atgcagaaac aagttgttca aagtacaaag ttatcgatgc
    21001 aagttaataa gcaaaatgca ctaatggcaa aacaacttgt gacacttaat aaaaaattag
    21061 aagaggttaa aggagagact gaaaatgctt aaattaattt caccaacatt cgaagatatt
    21121 aaaacatggt atcaattgaa agaatatagt aaagaagata tagcgtggta tgtagatatg
    21181 gaagttatag ataaagagga atatgcaatt attacaggag aaaagtatcc agaaaatcta
    21241 gagtcatagg ttataatctt atggcttttt aatttgaata aagtgggtgg tgtaatgttt
    21301 ggatttacca aacgacacga acaagattgg cgtttaacgc gattagaaga aaatgataag
    21361 actatgtttg aaaaattcga cagaatagaa gacagtctga gaacgcaaga aaaaatttat
    21421 gacaagttag atagaaattt cgaagaacta aggcgtgaca aagaagaaga tgaaaaaaat
    21481 aaagagaaaa atgctaaaaa tattagagac atcaagatgt ggattctagg attaataggg
    21541 acgattctaa gtacatttgt tatagccttg ttaaaaacta tttttggcat ttaaaggagg
    21601 tgatcaccat gcttaaggga attttaggat atagcttttg gtcgtgtttc tggtttggta
    21661 agtgtaagta atatttaaga gtcagtgctt cggcactggc tttttatttt ggataaaagg
    21721 agcaaacaaa tggatgcaaa agtaataaca agatacatcg tattgatctt agcattagta
    21781 aatcaattct tagcgaataa aggtataagt ccgataccag tagatgaaga aagtgtttca
    21841 tcgattatct taacagttgt tgctttatat actacatata aagataatcc aacatctcaa
    21901 gaagggaaat gggcgaatca aaaattaaag aaatataaag ctgaaagtaa atatagaaaa
    21961 gcaacagggc aagcgccaat taaagaagta atgacaccta cgaatatgaa cgacacaaat
    22021 gatttagggt aggtgttgac caatgttgat aacaaaaaac caagcagaaa aatggtttga
    22081 taattcatta gggaagcagt tcaatcctga tttgttttat ggatttcagt gttatgatta
    22141 cgccaatatg ttctttatgt tagcgacagg cgaaaggctg caaggtttat atgcttataa
    22201 tatcccgttt gataataaag caaagattga aaaatatggt caaataatta aaaactatga
    22261 cagcttttta ccgcaaaagt tggatattgt cgttttcccg tcaaagtatg gtggcggagc
    22321 tggacacgtt gaaattgttg agagcgcaaa tttaaacact ttcacatcat ttggtcaaaa
    22381 ctggaacggt aaaggttgga ctaatggcgt tgcgcaacct ggttggggtc ctgaaactgt
    22441 gacaagacat gttcattatt atgacaatcc aatgtatttt attaggttaa acttccctaa
    22501 caacttaagc gttggcaata aagctaaagg tattattaag caagcgacta caaaaaaaga
    22561 ggcagtaatt aaacctaaaa aaattatgct tgtagccggt catggttata acgatcctgg
    22621 agcagtagga aacggaacaa acgaacgcga ttttatacgt aaatatataa cgcctaatat
    22681 cgctaagtat ttaagacatg caggacatga agttgcatta tacggtggct caagtcaatc
    22741 acaagatatg tatcaagata ctgcatacgg tgttaatgta ggcaataaaa aagattatgg
    22801 cttatattgg gttaaatcac aggggtatga cattgttcta gaaatacatt tagacgcagc
    22861 aggagaaagc gcaagtggtg ggcatgttat tatctcaagt caattcaatg cagatactat
    22921 tgataaaagt atacaagatg ttattaaaaa taatttagga caaataagag gtgtaacacc
    22981 tcgtaacgat ttactaaatg ttaatgtatc agcagaaata aatatcaatt atcgcttatc
    23041 tgaattaggt tttattacta ataaaaatga tatggattgg attaagaaaa attacgactt
    23101 gtactctaaa ctaatagctg gtgcgattca tggtaagcca attggtggag tggtagctag
    23161 tgaggtcaaa gcgccagtta aaaacgaaaa gaatccgcca gtgccagcag gttatacact
    23221 cgataagaat aatgtccctt ataaaaaaga acaaggcaat tacacagtag ctaatgttaa
    23281 aggtaataat gtaagagacg gttattcaac taattcaaga attacagggg tattacccaa
    23341 caacacaaca attacgtatg acggtgcata ttgtattaat ggttatagat ggattactta
    23401 tattgctaat agtggacaac gtcgctatat tgcgaccgga gaggtagaca tagcaggcaa
    23461 ccgaataagc agttttggta agtttagtgc agtttgataa ttgtatatga tgaatcttag
    23521 gcaggtactt cggtacttgc ctattattta aaattaataa acagttaatt tttacatgaa
    23581 tatattaaat tttaaaaaaa caaacgtttt tagtatataa attattttgt gttcgtattg
    23641 tgtgctatga ttaaaaagtt gttatggtca actatatcgt ggttttatgt ttattatcaa
    23701 tcaaaata
//
LOCUS       DAKTIP010000019        23703 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAKTIP010000019 DAKTIP010000000
VERSION     DAKTIP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.019"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(249..728)
                     /gene="rlmH"
                     /locus_tag="O0305_000620"
     CDS             complement(249..728)
                     /gene="rlmH"
                     /locus_tag="O0305_000620"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCY0273778.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(1096..3414)
                     /gene="adsA"
                     /locus_tag="O0305_000621"
     CDS             complement(1096..3414)
                     /gene="adsA"
                     /locus_tag="O0305_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCY0273779.1"
                     /translation="MKALLLKTSVWLVLLFSVMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHHSGKEAATNVSASAQGTADDTNSKVTSNAPSNKPSTVVSTKV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSKSGKDTQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(3642..4442)
                     /locus_tag="O0305_000622"
     CDS             complement(3642..4442)
                     /locus_tag="O0305_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0273780.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAAHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(4832..5620)
                     /locus_tag="O0305_000623"
     CDS             complement(4832..5620)
                     /locus_tag="O0305_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCY0273781.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(5621..6955)
                     /locus_tag="O0305_000624"
     CDS             complement(5621..6955)
                     /locus_tag="O0305_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273782.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLNSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(6948..8774)
                     /gene="walK"
                     /locus_tag="O0305_000625"
     CDS             complement(6948..8774)
                     /gene="walK"
                     /locus_tag="O0305_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCY0273783.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(8787..9488)
                     /locus_tag="O0305_000626"
     CDS             complement(8787..9488)
                     /locus_tag="O0305_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0273784.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(10108..10180)
                     /locus_tag="O0305_000627"
     tRNA            complement(10108..10180)
                     /locus_tag="O0305_000627"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10145..10147),aa:Asp,seq:gtc)
     gene            complement(10188..10262)
                     /locus_tag="O0305_000628"
     tRNA            complement(10188..10262)
                     /locus_tag="O0305_000628"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10227..10229),aa:Glu,seq:ttc)
     gene            complement(10692..11975)
                     /locus_tag="O0305_000629"
     CDS             complement(10692..11975)
                     /locus_tag="O0305_000629"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCY0273785.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(12253..13653)
                     /gene="dnaB"
                     /locus_tag="O0305_000630"
     CDS             complement(12253..13653)
                     /gene="dnaB"
                     /locus_tag="O0305_000630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCY0273786.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHVFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(13685..14131)
                     /gene="rplI"
                     /locus_tag="O0305_000631"
     CDS             complement(13685..14131)
                     /gene="rplI"
                     /locus_tag="O0305_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCY0273787.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(14128..16095)
                     /gene="gdpP"
                     /locus_tag="O0305_000632"
     CDS             complement(14128..16095)
                     /gene="gdpP"
                     /locus_tag="O0305_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCY0273788.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(16110..17048)
                     /locus_tag="O0305_000633"
     CDS             complement(16110..17048)
                     /locus_tag="O0305_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCY0273789.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(17339..18307)
                     /locus_tag="O0305_000634"
     CDS             complement(17339..18307)
                     /locus_tag="O0305_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCY0273790.1"
                     /translation="MTNYTVDTLNLGEFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(18367..18465)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18670..18999)
                     /locus_tag="O0305_000635"
     CDS             complement(18670..18999)
                     /locus_tag="O0305_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCY0273791.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(18996..19691)
                     /locus_tag="O0305_000636"
     CDS             complement(18996..19691)
                     /locus_tag="O0305_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCY0273792.1"
                     /translation="MTTHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIVL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMEQ"
     gene            complement(20341..21627)
                     /gene="serS"
                     /locus_tag="O0305_000637"
     CDS             complement(20341..21627)
                     /gene="serS"
                     /locus_tag="O0305_000637"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCY0273793.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(21717..21933)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22006..23520)
                     /gene="hutH"
                     /locus_tag="O0305_000638"
     CDS             complement(22006..23520)
                     /gene="hutH"
                     /locus_tag="O0305_000638"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCY0273794.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSTLTTRQSEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYDEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         6999 a         4699 c         3592 g         8413 t
ORIGIN      
        1 aatttactat atttagttgc agaaagaatt ttctcaaagc tagaactttg cttcactata
       61 agtattcagt ataaagaata tttcgctatt atttacttga aatgaaagac tgcggaggct
      121 aactatgtca aaaatcatga acctcattac ttatgataag cttcttaaaa acataacagc
      181 aattcacata aacctcatat gttctgatac attcaaaatc cctttatgaa gcggctgaaa
      241 aaaccgcatc atttatgata tgcttctcct cgcataatct taaatgctct gtacacttgt
      301 tcaattaaca caacccgcat catttgatgt gggaatgtca ttttgctgaa tgatagtgcg
      361 tagttactgc gttgtaagac gtccttgtgc aggccgtttg atccgccaat gacgaaaaca
      421 aagtcgcttt gcccttgggt catgcgttgg ttcaattctt gggccaatcc ttcggaagat
      481 agcatctttc cttgtatttc taatgtaatg actgtggatt gtggtttgat tttggctagt
      541 attcgttggc cttctttttc ttttacttgc tcaatttctt tgtcactcat attttctggt
      601 gctttttcgt ctggaacttc tatgatgtct atcttggtgt atgggcctaa acgtttttca
      661 tattctgcta tggcttgctt ccaatatttc tcttttagtt tccctacagc taaaatggtg
      721 attttcatgt cgtttggtcc tccaaattgt tatcaacttt ccagttatcc acaagttatt
      781 aacttgttca cactgttccc tcttattata ccaatatttt ttgcagtttt tgatattttc
      841 ctgacattta tgcccgattt ttagtgtata acctgtgtat ttgtggataa ctctgtgggc
      901 attgtgggta tttctcttta aattgtacac aattctggat aactatcccc atttctgtgg
      961 ataaacacct tgatgtctta ttacttatcc tcgtttttat atcccatatt tatcaacggt
     1021 tatcgctttt tttcataaaa aaacactacc gtacctctta tttaagaaat acagtagtat
     1081 tattttcaat atattttagc tagcttttct acgtcgtaca gtcgcgatta aacctatacc
     1141 tgctaataat acaaacatcg cttctgggct tgaactttga ttagttccag tttttggtaa
     1201 ctgtttctca tgcgcgctac ctttaggcac tgacattcta gccaattgtt tcccactctt
     1261 gcttgataca gtagctcctt ctattgtgcg accagaacct tctgtaccgc tactaacagt
     1321 tcctctatgc gctagcaaca atacaacttt acctggagct ggttttttcg ctggatccat
     1381 cactttgtcg tcaccaattg gttgtgtatc tttaccagac ttactacctt tgctaccttg
     1441 ttgtccttta gctggttgtt cacttactgc tggtttacct aataacatac gttgtggttc
     1501 tgtcgtatca tacttagcta agttagctgt ttttaaataa cttgctagta cttgatctaa
     1561 tgaaatacct tcttctctag gaccaccgaa catactatat ccgtcgccac ctgatgctgt
     1621 gaagtcattc atcgttacgt gatatacacg ttttaaatca atattttcaa acttacctgt
     1681 ctctttattt aaaatttgaa tagcattaat tcgtttgcca gacggtttat ttatatcata
     1741 gtaaacacgg attgaatcag agatatgtag taaaccgcca ttcgctgtta acactgtctt
     1801 accgtccttt tgtgttgttg gtgcgcctaa actatgttcg aaagccgtcc agacgtctga
     1861 accttttaca tcaatttgcg caatcgtatt tccaaatggt aatactgaga ttaaatcata
     1921 gcgtgtcacc ttaccttttg cgatagaggc acgaatacct ccaccatttg tcacggcaaa
     1981 gtcagtcttt ttagagaaat tcttaacgcc atacgcttcc atagcatctg caatcgcgtt
     2041 tcctaaattt gtttcacgcg ttctaacgtc atctctttct cctttgaaat caatggtatt
     2101 gtttggaata attacctctg cagtttgtgc tctaaatgtt tgatcagctt gattaatttg
     2161 ttcagctaat gctttgttcg gtgttacatt ttcaacgtct ttaacattaa tcaatgacgg
     2221 tttaatattc gatacctctc cattgcgata attaaatgta atcttaccga tattcgcaag
     2281 tgctgtacct gtttgtgcca atgcatcatt gttataaatt tgaccatttt gaagtactgt
     2341 atgtgaatga ccatcaataa ctgtaatacg tttcttcaat tgtggatttt gacttaattg
     2401 tttcactaag taatcaccac gccatgtttc ttgtgttgaa ggatcaattc ctaaatgtga
     2461 tataacaaca aatgtatcta cgtctttata aatacgcatc atttccgctg tcacactttg
     2521 taatggatct ctaaattcaa cgcctttaat gccttcaggt cttgttttcg tctttgtttc
     2581 tggtgtcgtt acaccaataa ttccataacg aataccattt tttgttacaa tcgttgaagg
     2641 cttaaacgcg cgttttccat ctttataaac gttagtactt agcatcggga agtctaacat
     2701 accctctaac tttttcaact gatcgtatcc aaagtcaaat tcatggttac cgactgccat
     2761 agcatcataa cctactgcat tcattgcttt agccatttct tcacctttag actggtttga
     2821 aagtggtaaa ccttggaagg cgtctcctgc gtctaacatt aaatcaggct tttcttgttc
     2881 ttttactgtt tttaatttag ccataccgat gacacgccct ttttcttcgg ctagtcggcc
     2941 atggatatca tttgtatgta atattttatg tgttgttgtt tgtggtgcat tagcagcaaa
     3001 cattcgtggt gaaagtgatg ctgttttagc atttgataat ttgaacgttg ctgtgtgagt
     3061 tggtttttgt gttgaggctt gttgtgtatc tacgtcgcgt gtttcgttta cttttgttga
     3121 aactactgta gatggtttgt tagatggtgc gttggatgtt actttgctgt ttgtatcatc
     3181 agctgttccc tgcgctgatg ctgatacgtt ggttgccgct tctttgccag aatgatgagc
     3241 cgcttccttt gttggcggta cttgttgctt atctgttgtt gctttgtcta tcgttgttac
     3301 tgcatgtgct ttcattggtg tatgctgctc agccgcgttc gagacttgcc ataatcccat
     3361 tacactaaaa agcaaaacga gccatacact tgttttaagt aataaagctt tcatttcgtt
     3421 ccctccatta actgtcatac accaatatat tatcataatc cttatattaa aatgattgat
     3481 tttatgtaaa ttatttataa attacattta atatcgaatt attttacaca tttcaatcaa
     3541 gccgattcaa ctcaacaaca gccatttccc accaacaaca taaaaaagca cccgataaag
     3601 tcgtctcaca gcaatgcgga actttatcgg atgactctca tttatattgt atatattggc
     3661 gttggaatag ctttatccgt atcacatagc aatacttctt tttccgtatc aatatcgtgt
     3721 tcgttcaata cttggccaac actcatacgc gccaaatctt tcatgttatt gtcttgtgat
     3781 aaatgcgata ggtaaatacg tttcgtgtta cctgtaatca cgtctgtcat tgcatgagcc
     3841 gcatcctcat tagatacatg acccatatcg cctaaaatac gttgtttcgt cttccatgga
     3901 taacgacaca ttctcaacat atcgacgtca tgattactct caaaaataaa cgcatcgctg
     3961 ccacgtatca tacctttcat acgatcagac acgtaacccg tatccgttaa aatcgtaaac
     4021 ttcttatagt tattatggaa aatataaaat tgcggatcta ttgcatcatg tgacacgtta
     4081 aacgattcaa catcgaaacc tgcaatagat tttgtttcat aaggattaaa aatgaatttc
     4141 tgatccatag ggatgcgact atctttcttt tcaattgcct gccaagtctt ttcattcgca
     4201 taaattggca attgatattt acgcgccaaa acacctaatc ctttaatatg atcaatatgt
     4261 tcatgggtta ctaaaatacc atttaaatct tgaatattac ggtcaatttg actaaacaat
     4321 tcttccattt tctttccagt caaaccaaca tcaactaata gactaccttt ttcattttct
     4381 acaaaagtgg cgttacctgt actaccactt gctaatacac tcatgcgtat caagcggctc
     4441 atcctttctc tttaaacagt caatctctct atatgactgc aatatacatc agtgcaaata
     4501 tacgattttc cctacagaaa ctatcagtat attagctgtt ttgtatacct ttcctattat
     4561 gtaactttag ttcatcgacc ggtttcttat atttaaattt tgataatcac gtgcaatgaa
     4621 tttcttaatt attataacaa tgattagaca aatactaaag tttttaaaat aacagttaca
     4681 ctaattttac aatgcggttc tacttaaaat atgaataaat gcgcacgttg gagacgcaaa
     4741 aacatcagcc ataataaacc gtaccagcaa aaaagctagc accgcttctc acatatgatg
     4801 agcttgcaat gctagcttat tacgattcat attaatgatt aataatttta gggttattat
     4861 ttgtcgcttc gacatagtaa gtattcgttt tatccttacc gtcatgcttc actttaattt
     4921 cccagtttgg ttgtaacaat tgaacattcg tttcattcac gactgagtag taaccgagtc
     4981 tagcattaat gacttcatca ccttttttca agtaacgatt ataatataat gcctcgattg
     5041 cttttctcac accaatcact tgatgcttct tatctgcacc atctgtgggc ttaatgtcat
     5101 ccatcattga ttgtttataa ctagtcgctt tgttatcttc tatattaaag ttcaacatcg
     5161 ctttactgtt atttaaaatc ggaaaatcat catacgtttg ttcatatttt acagaatctg
     5221 aactaatctc gcttaattga tattcagcac ctttaaatac gcgcttgtta acataatctt
     5281 tcaaatcttt taagttattg tcactgacct ttaccgattg actaatgttc gcattcaata
     5341 attttccacc atctgatgtg gtcaaatccg aatcgccttt agctttagaa ctaaagtctt
     5401 ttgatcgccc tgtaatttgc tctaatttta tacctttaac tgatttattc aatatactag
     5461 tcggtacttt aatttcttct tgctgaaaat taacttcatt gttactctcg acttcattaa
     5521 tgtgtgagcg attgacttta ttaacataaa tcgatactaa cacgatgttg acaagaataa
     5581 acacgaaaat gaaaagtgtc tttgtcagtt tccagttcat ttattcaagc ctcccatcgt
     5641 tataaacata ccattcgcca tcatattcta cataccaacg cggtacgagt tcactgttaa
     5701 tctgcacttc aatgtgatta tgatctgaat tatcctgcat ttcgtaaccg atagcgatgt
     5761 ttgttacttt ttcaaaatta atatcactat tgttcgctaa gcttgaacgt acagactcta
     5821 atttcggcaa cgatttatta tcctcactat ttaaaacaac gtcggtgcgt aataacgaac
     5881 gacgatagtc aaagacgcct ttttcacccc aagtgacttg aatttgatta gaaccttctt
     5941 tattaaacgt tggataacca ttaaggaaac gttgatatgt taactcgcct gactgattat
     6001 tcgtactaaa caatctaaag tcttcgttta agaaaccacc atgaccatta ataaaatcaa
     6061 aggttcctgg aatcgtttct tccattttgc tggaactcgc ttcatcttcg gacaggtttt
     6121 tataatgata tttttcattt ttatcgttat agtttgcgac acctgtatta ttgttgtagg
     6181 ttgtaacacc actctttgaa ctacgaacga tggttgaatc gtcaaatagt atagcattca
     6241 ttttctcaac actaatcgtg ttaaatacca tgcgatatgt ttttaacttt tcaggtttac
     6301 ttggtgcaaa aacatgcgtc gtacgatcaa ttgtatcttt gtttgtgatg atatctgtgt
     6361 atggttgcat atcttttttc actgctgcta atgcatctaa aaaatgatca tttttcgttg
     6421 tagttgttaa ttttacgtaa tcgtggcgat ctttgcttat agcataaagc acgatattat
     6481 catcagcatc atgatctatg accaaacgat tgaaattgaa atgatttggt actttcgcat
     6541 tcatgttcag tacttgacca agatatgttg ataacggtaa atcatacgta aaatcgaata
     6601 atataaaatc actgttcaaa tcaggaatca tcaagttatg atcacgacga acatgttcca
     6661 cggacttcac ttctttattt ttcaaaggtt tcgtcagttt attcacatta gataccgtcg
     6721 caatcgttcc ttctggatga tcatttttcg aatgaataat ctgaaatggc gtaatagttg
     6781 tatccatttt ggctgtcata ggtgtcgtta aaggtttcgt ttcactcttc ttactatctg
     6841 tattgtcgac atttgcaata tcaggagaaa agttccatac catatatgtc aatacgacac
     6901 tcatcaagac gagtagtgct aaaatgacag atttaatatg ctccttatta ttcatcccaa
     6961 tcaccgtctt caatgacttc acatggaagt gtgataaaga tagatgtacc ttgaccttct
     7021 acactgtttg cccaaatacg accattgtgc gcttccacaa tctctttcga aatggctagt
     7081 cctaatccag taccacccat tttacgcgta cgtgccttat ctacacgata gaatcggtcg
     7141 aatatcttat cgactttatt gataggaata ccaatgccat tatctttaat acgaatcgtc
     7201 attcgattat aaagtggatt ttgtttcacg tggaactcga cacgtttatc gcctctagaa
     7261 tatttcatcg catttgtaat gacattatca aatacttgcg tcattttatc aggatcaaat
     7321 tctgtgaaaa tcgtcttttt cggaatatct cgaataaatg ttgtatcttt cgcagacatt
     7381 tcatgtcgat taataatttt attaatgaac atgttaaagt cgataatttc tttgttgatt
     7441 tgatcagact cattatccat tttagataac tgtagcaagt cattgaccag tcgaatcatt
     7501 cgttctgttt cttcacgggt aacagataaa aattgtggcg caagttcctc atctttccat
     7561 gcaccttctt caagtgcttc aatgtaacta ttcatagaag ttaaaggtgt acgtaactca
     7621 tgtgatacat tggcaacaaa ttcacgacgc tcacgttcaa cttgttgttg ttcagttacg
     7681 tcatgtaaca cagcgatata accagttaca aatcctgttt cctgcacaat cgtactaaag
     7741 ttaacacgtg cgattagacc ttcttcttca tttaaatcta ataagaaact atcattattc
     7801 tcttgaattt cttccagttt aaattcatct tcaagactta atacacttaa catgtaatat
     7861 ccgatgatgt cttctttcgc cataccaagc atcttgagtg ccatatcatt gacgatacga
     7921 atacgtccac ggcggtctgt tgcaataata ccatcactca tatgggtgat aactgagtcc
     7981 agtctacgtt tctcactttc agtattagcc tgcgcttctt gtacacgttt agacaagtta
     8041 ttaaatgcta aagctaattc gccaatttca tcattaccat aaatcttcac acgttgcgta
     8101 tagttacctc tggacatttc gaccgtctgg ttacgcatat cggtgattgg tttggtaatc
     8161 gttcgcgcta taaagaatcc taggatgact gtgattaata atgaaatagc tgtaccaaca
     8221 atgaatattt gatttatatt atttaattgg ttataaacgt cattaatttt tgattcgata
     8281 taaatattac caattacctt tttatcgact ttaactggga tattatatac ccagacacgg
     8341 tccttaccac cgccataatc ttttaaaatt aaatgatcgt ttgattgtcc tagtgatagt
     8401 gctttttgga cagaactatc attcgctttt tgattgatta gactacggtt agactgcttc
     8461 gtcgtcgcaa taataatttg gtctttatct ataaaacgaa tttctccaat ttcttgacgg
     8521 ttggcatact cacttaataa attttgaata tctttttgtg catttacgga gcccttttcg
     8581 tcatatactt tttcaatact aatttctaat tgtttcgcgt actgcgtaat attcttctta
     8641 aaattatcaa gcagctcttt ttcaaggtta tttgtaaaat ataacccgat aatttgcata
     8701 ccaatgataa tcagtaatac ataaacaatt acaagtttag tatgaaggga ttgtagttgt
     8761 tttagccact tcattcgttt cgacctctac tcatgttgtt ggaggaaata tccaacgcct
     8821 ctacgcgtca caatatattc aggatgtgac ggatcatctt caatcttttc acgtaaacga
     8881 cgaatcgtta catcgaccgt acgtacatcg ccaaagtaat catagcccca tactgtttgt
     8941 aataaatgtt cacgtgtcat tacttgtccc atatgttttg ataaataatg gaacaattca
     9001 aattcacgat gtgttaattc aatatcttcg ccacgttttt taatagaata tgcgtctgga
     9061 taaatcacaa tatctttaat tgtgatttca ttcgttacat ttccagtgtc ttgtgctggt
     9121 tgtgagtaat gacgacgtaa gttcgctttc acacgtgcga ttaattcacg cgtactaaac
     9181 ggtttcgtta catagtcatc tgcacctagt tctaaaccaa gcactttatc aatttctgaa
     9241 tctttagcag taagcattat tattggcatt tcgtattttt tgcgcacttc acgacatact
     9301 tccataccat cacgaccagg taacatgata tctagtaata cgatgtctgg ttcttcttca
     9361 taaattaagt cgactgcatc attaccatcg tatgcacagt acacatcgta tccttctttt
     9421 tttaagttaa attctaaaat atcagcaatc ggtttttcat catcaactac aacaactttt
     9481 ctagccattt gcataaacct cttttcttaa atcattaata ttattttatc attttttgtt
     9541 ctttatcgtt tatgtttgtg taaaaaatca caggatgtcg cttactattt ttgttattgt
     9601 gcgcattttc cgtcttataa cgtatagtaa cctattatat aatttaccat ttataagcta
     9661 ttaattctat tttccaatat aaacttatat attactattt tttattagtt tctcacggcg
     9721 gattgtgaca atgatattta acacaatgat gaggtctcta ttccaataat aaaattagga
     9781 taatggcaac ttctagttgc acgtcattgc tcagaaaata aatatcatta ttcgttattc
     9841 gttactttaa caattgatgt tatggaggac tggtggcata tcacaacgaa gaaatatgtt
     9901 gacagtagca tatgtttgta cgtctaataa agattttatt gtctacgttt atctttcata
     9961 tccaaccact aagttacttg aggttcatac attttaaaga aaatggacga acagataaat
    10021 caatatgatt atcattcgtc catatatctt ttattaatat aatattaaac gcaaaaaaat
    10081 gcctatcctc ttggataggc atttgtacgg tctcgacggg aatcgaaccc gcgatctcct
    10141 gcgtgacagg caggcgtgtt aaccgctaca ctacgagacc taaaaaatgg tgactcctac
    10201 gggactcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaaggag
    10261 ccgttcttaa gcacaagata gattatagca cagccaatct tgccatgaca agatattttt
    10321 tacataattt tgctattatt ttttgaagaa tgttataagt gaaaaattgc attttattgg
    10381 cagtctattc attataataa ttacgctatt tttgaaattt ttctatttta ggtgctttca
    10441 tttacactta cgtctactct attttaatag ctattttcga catcagactt cttacattca
    10501 agtatttcat cttaattttc gttaaatatc atctaatctt ctaaattatg actagcaaat
    10561 atcgccttta cgttagtatt ttttatctaa ataatcgaat cgatacagaa gcaagcataa
    10621 caaaagcctc ctaccaataa agatagaagg ctcatgtatt tataatcatt gtgtatgact
    10681 tatataaagt tctaccacaa ttcttttaat aggtttgttt gttctctatc tggaccaact
    10741 gagaagatag aaatttgtac attacataat tctgaaatac gctctaaata tttacgtgca
    10801 ttttcaggta attcttctaa agtacgcaca tttgttacgt cttctgtcca acctggtaac
    10861 tcttcaaaga ttggtttaca acgttttaat tgatctaagt ttgctgggta ctcagtaatt
    10921 tctttaccgt ctaattcata agctgtacag attttcactg tgtctaggcc tgttaaaaca
    10981 tcgattgagt taatagataa atctgtaata ccacttacac gacgagagtg acgtaataca
    11041 actgaatcaa accaacctac acgacgtgga cgtcctgttg ttgtaccgta ttcacgacca
    11101 acctctctaa tatgatgtcc atcttcatcg aataattcag tagggaatgg accatcacca
    11161 acacgtgatg tataagcttt acatacacca attacctttg aaacgaatgt aggacctaca
    11221 cctgtaccaa cagtaacgtt acctgcaatt ggattacttg atgtaacgaa tggatatgta
    11281 ccatggtcga tatctaacat tacaccttgc gcaccttcga aaagtacctt ttcatctgct
    11341 acaaatgcat cgtctaagat ttttgatgtg tctgttacaa attcttttag acgttgacct
    11401 gctgcataat attcttcaaa gatatcatca aatgatggac atgtttcgtt aaacatacct
    11461 ttgaaatatg cttgtttata ttcaatgttt gattttaata atctttcgaa tgtttctttt
    11521 tcaagtaaat ctgccatacg aataccgata cgttgaactt tgtctacata tgctggaccg
    11581 ataccttttt tagttgtacc aatcttattg tcaccacgta aacgttcttc atattcatct
    11641 tgtgctaagt gatatggtaa aatcacttgc gcacgattag atatacgtaa attacttgta
    11701 ggaatgccac gttcatttaa tccgtctaat tctttcaata gtgcaactgg atcaacaacg
    11761 actccgttac cgattaccgc taatttgtct ttgtaaaaga taccagatgg tactaaatgt
    11821 aatttatatg tttctccgcc aaattgaatg gtatggcctg cattattacc acctgaaaaa
    11881 cgcgcgataa catctgactg ttctgccaag aaatccgtta tttttccttt tccttcgtct
    11941 ccccattgtg tcccaactac tacgattgat gacatgtgag cacctccaag tttttctcat
    12001 ttaaccattt gatattctac caatatcgag accataatgc aaataaaaat cgaacattga
    12061 ttttcggcct ttttaaaaga aaaatcattt taacaaatcg ttttcaacgt cctaaaaacc
    12121 ctttaacaga ggcgttgaca gtactaattt atcactttca atacacaaac ttaacatata
    12181 tcaacccgtt cagaacaaaa taaaaaccgt acaattttat catcttaatg attattgtac
    12241 ggaaaaactt ttttacatca tatctgcatg tgcataatcg atatcggtaa atttattata
    12301 ttgtttcata aaatgtaact taactgtgcc tgttggaccg ttacgttgct tagcaatgat
    12361 aatttcaatt tcaccgtttt catcattcgt ttgtggctcg aaaccaccat catcgtcatc
    12421 atcttcatcg ccgccacggt tatagtaatc atcacggtat aagaatgcaa cgatatcggc
    12481 atcttgctca atcgaaccag attcacgaat atcactcatc attggacgtt tatcttgtcg
    12541 ttgttcaaca ccacgagata actgacttaa tgcgataact ggacattcta attcacgggc
    12601 taatgctttt aatgtacgag agatttcaga aacttcctgt tgtctgttat cggacgcacg
    12661 tgaaccacta ccttgaatca actgtaagta gtcaatcaca atcatgtcta agccatgttc
    12721 ttgctttaat cgacgacatt tagaacgtaa atcattaatt cgaatacccg gtgtatcatc
    12781 aataaaaatc ttcgtacgtg ataatttacc taccgctata gtaaaacgac tccaatcttc
    12841 ctcagtcata gtacccgttc ttaagcggtt tgagtcaaca tttccagaac tacaaatcat
    12901 acgtgtggct aactgatcag cacccatctc tagcgagaaa ataccaactg tatacatatc
    12961 ttcatgcgtt gcaacttttt gtgcaatatt aagtgcgaac gcagtcttac ctacagatgg
    13021 acgcgctgca aggataatta aatcatttcg gttgaaccct gctgtcattt ggtctaaatc
    13081 tcgatatcct gtaggtatac ctggtgtttg accactattt tgatcaagct cttcagctgt
    13141 ttcatacact tgtcctaaga cgtctcgaat gtctttaaag ccatcgcttt cacgagaaga
    13201 tgatagctct aaaattcgac gttctgcatc acttaaaatc gcatctagtt caagttcatc
    13261 attatatcca tcattggcaa tactatctgc agtttgaatc aatctacgtt ttaatgcatg
    13321 cttagaaacg atatcagtat aatactgaac atttcgcgtc gttggtacat ttgtagataa
    13381 ctctgcaaga tattgcgggc cacccgcttc attcaacgta ccttccgtcg ataattgatc
    13441 catcaatgtt acaacatcaa tttctttatt atcttcattt aagtgcatca ttgcacggaa
    13501 aacatgttga tgggcacccc tataaaacga ctcaggaagc aaaacttcct gagtagtatt
    13561 aatcaattct ggatctataa taattgaacc taagacagac tgttcagctt cattgttatg
    13621 cggcatttga ttttgctcat acattctatc catgaatggt tacacctctt atttcaatcc
    13681 aactttattg ttcaactgtg tgtacgcgaa ttgtaccttc aacttcttta tctaatttaa
    13741 caggtacatt cgtatatcct agggaatgaa ttccatttgg taaatccatt ttacgtttat
    13801 caattttaat atcatgttgt gcttttagtg cttcggcaat ttgttttgta cttactgacc
    13861 caaacaattt accaccttca ccagtttttg ctgatacttc aacttcaatg tttgataacg
    13921 tttcttttaa tgctttagca tcttcaattt cttgttggcg ttcttgtttt gcacgttttt
    13981 tctgtaactc taattgttta aggttacctg gtgttgcttc tacagcataa ttctttttca
    14041 ataagaagtt atttgcataa cctactggta cttctttaac ttcacctttt ttacctttac
    14101 ctttaacatc ttgtgtaaaa attactttca tgcatcttca ctcctactta attgttctgt
    14161 aattgcttgt tgtaattgtg ctatcgcctc ttcgactgtc acacctttaa gttgtgttgc
    14221 cgcattggtt aaatgtccac cgccaccaag tgcttccatt gttaactgga catttactga
    14281 accgagtgaa cgcgcagata taccaatcag attatcttca cgtctcgcaa caacatatga
    14341 tgcttcaata ccttctaaac ttaacagttc atctgctgct tgtgcaactg ttactggatg
    14401 ataaatttta tcgtctgaac catgcgcaat ggctatgcca ttatcttcaa cttttacagt
    14461 tcgaattaat tcagatcgat taatgtaagt atccacatca tcttttaaga aatgttgcgt
    14521 taaaatcgta tctgcaccat gtgcacgtaa ataactcgct gcatcgaatg ttcttgatcc
    14581 tgttcgtaat gtaaagtttc ttgtatctac aataatacct gcatacatca ctgttgattc
    14641 aagacgtgtt aaacgttgtt ctgttggttg atattccagt aactctgtta ccaattcagc
    14701 tgtcgaactt gcgtatggtt ccatatatat caacaatgga ttagagatga agctttcacc
    14761 acgtctatga tgatcgataa caactttacg gtttgcttta tttaagacat tttcatctaa
    14821 aaccagttcc ggtttatgcg tatcaacaat cactacggtt gtcttagatg tcatcatatc
    14881 ccaagcatca tctgatgtaa taaatcgctc tcttaactct ggctttttat ctatttcgtt
    14941 catcacgcgt cgtaatgttg gatcaatgtc agtctcattt aatacgatgt atgcttctaa
    15001 attattcatc attgcaaatc tagacacacc gattgctgca ccaattgcat ctaagtcagg
    15061 acgtttatgt cccatgataa tgactttgtc accctctgca aggatatctt ttaacgcatg
    15121 tgagatcaca cgtgctctta cacgagtacg tttctccatc gggtcagtct taccgccata
    15181 gaaacgcaca ttaccattaa tacttttaat tgcaacttgg tcgccaccgc gtcctaatgc
    15241 taagtctagg cctgattgtg ataattcacc taagtcgatt aaattttcag taccttcacc
    15301 aacaccgata cttaatgtta attgggcacg ataaccaaca cttttttcac gtaattgact
    15361 caagatatca aatttagatt cttctaagtc agctaatatt ttttgattta aataggctac
    15421 gaattgatcg gaactgtatc ttttgaaaaa tatattatac tcagttgccc atcgactaat
    15481 gacacgcgtt accattgagt tgatttccga acgctgcgta tcattcatat tttgcgtaat
    15541 ctcatcgtag ttatctaaaa ataatgtcgc aatgattggt ttagaatttt catatagttc
    15601 atttgtttgt acttgttcag ttatatcaaa gaaatagagg cagtgatcat tctcagaata
    15661 acgtacttgg aaatgatact gattatattc tatttcaacg gatttcactc tatctaattg
    15721 ctttaaaatg tttggaaata cttcatttac agattcagaa atgacattcg cttccatatg
    15781 atctgtcata aattggttaa cccattcgat gtgatcattt tcatctaaaa caatgatacc
    15841 aattggtaaa tgtttgattg ctttattatt tgttgttgaa atttgagcac tcaaaccatc
    15901 tacataacta tccattttca ttaaagcttg tctgaataaa atgatgctaa caataatcat
    15961 cacgacaaga acgatagatg caattagtgc tataagacta ttaaagataa accatacacc
    16021 cattaaaaca attgctgtga tgatcatgat gacaaatggt attagtaaag ctttcttagt
    16081 ggactgccga ttcattattc cacctctatt cactttttag aattattttt catgattcgc
    16141 ttcaaattca aacttaaatc gataacacca agtagtccta caatatgtgt cgtaggtgtc
    16201 agtattgtac cgataaccaa tagtaaaatc gttactgcat tcggcaaacc tttcgcttta
    16261 ccaaagaaat gaataacact taaaccttga atatacatta ctaatgataa cacaagttgg
    16321 aagtttaaaa gaatgctctg gaacacactc ggttgacctg taaataataa acatatgata
    16381 acaataatgt atatccataa taaaataccg ctcatttgcc acgcgaaaag tggcttaaat
    16441 acaggtgtag cgattttaaa ttttcgtaaa atcggaaatg taacgattaa gttaattaag
    16501 acgattaaaa atgtaatgat aatgatgaaa cctggtaatt gaacggtcgc ttgtctaaac
    16561 ccttcttcta atatttgggt catattcgca tcggcaccgc tcatcgtaat cgcttcatgt
    16621 aatgtttgct tgaaaggttt tactatgctc gctgatggtg gaatccttcc gaatgtttgt
    16681 agtaacataa aagcgattaa tgaaattaag ctcatcgcta ctgttgttac gtataatatt
    16741 ctttctttag acgttctttc tttgagcaat tgaccaataa ttaaacttgc aattaagact
    16801 aatatgatgg cacttaaaac gaaagtatta cctaaaacag ttgttataat tactgtaata
    16861 agtgcactaa tcccgaaaga ttgtattgat ttattccata aaacgatacc tggtatggtt
    16921 gcaaataatg caaatattag tcctaaacca ggcactagat ataaagctaa agcgacaaat
    16981 accaacgcaa tcgttgcaat tattgttgct ttaggttgta tttttgaaaa cacataagcc
    17041 actcccatat ttttaactat agctattatt ttaacctctt taatgaaaat taacaattta
    17101 tagattgtat gcttctattt catttaattg aataataact ttcatgtttt ataagtaatt
    17161 aacatactca tttgaatcgc ttttgtgtgc tttcattttc aacatgatta tttaatccca
    17221 ctacatagca atcaagcttg atttagattt acaatacatt tccactctca tgtactctag
    17281 atgtttttga atatgataac tgtgatttag tggcttcatt ctttgaaaat atatattatt
    17341 acttacgctt aaaatgcttt aaatttaaga aatgatataa gttaggtgcc caggtactaa
    17401 agtttagtag gaatccatca tgcccaacat tatcaggcac gaagaaatga cgatgatatt
    17461 taaaacgttc acctaatgca cgaacttgat catccggata tagcaaatca tctatgaacc
    17521 ccatcgttaa cacttttgtt tctaaatttt taaaaacatg cgttacgtct gtgcgacctc
    17581 ggtcaatgtt gtgactatcc aatacatcta gcagtgtcag ataacaattc aaatcaaaat
    17641 gttctttaaa tttattacct tgatgttgtt ggtatgcgac tacttcatcc ggcgtgaaac
    17701 gttcatcata actttttgat gatcgatatg tcaaaaaacc taattggcgt gcaatactta
    17761 gaccttcctt accaccaaga tgaatggctt gccttgcaat ttcattgaaa gctctactat
    17821 aagatgatgt tcgacttgtt gcagcaagga taatggcttt atctacttca aactgttgat
    17881 tgtagagtag ttccattgct tgcatacctc caagacttcc ccctattaaa atattaatct
    17941 tatcataacc aagggcttgt atacctcgtt cattcgctct gactatatct cttaatgtta
    18001 attttttagg aaaatgaggg tcgtttaaag gtgaacttga accgaaagga ctaccaataa
    18061 catcaaatgt taaaaattga taatcgtgaa tgggtatata tcccccatca ataatttctc
    18121 gccaccaacc cggataatca tctgttccat atgttaaatg attgccagtt aatgcatgac
    18181 aaactacaac taatggttgt ccatgataac cgacatgctc atatctcaaa cgcaagttat
    18241 ctatgacttc cccagattct gtaataaatt cccctaaatt taaagtatct actgtgtaat
    18301 ttgtcattgt tctttcctcc ttaaacaaaa aaacttctca ccctattgaa aagtaagaag
    18361 tctttatact tatcattcga gtaactcgtt ggttttagca ccgtgctata aagtcggttg
    18421 ctgaagtatc acagggccaa gtccctcaac ttctcacaat aagaaatatg aaattgttat
    18481 gtgttagttg agattcagtg atgaattact tttatcattt aaaatgttgt tatcattgtc
    18541 atgcgttagc caaatcgctt acgtatacac gattcccaat cttaacatag acgatttgta
    18601 tatcagaatt ttctgattac taacagttta cctaagttta aatatctgtt caatgatttt
    18661 cagttatttt taaaagaaaa atcgtaatgc tgccatgata acaatcccac taataattgt
    18721 aatagttaaa ctacgcgtga ttatagataa aataaccgtc ggaatgagcg cgataatgta
    18781 agggatgttt aatgtatacc cctcaccatg aggcgtctgt tgaataatgc tgtcaatgac
    18841 aagtgccgta aatagtgtga ttgggataaa tgatagccat cgaaccacga catcaggcaa
    18901 ttgcactttt gaaatcatga taaaaggtat aattcgaatt aatagcgtta cgataccaca
    18961 caataaaata agtattaaca tgttcatatg agttatcatt gttccatcat cactcctaac
    19021 gctgctgaaa ttgtggctgc aattaatatt gctagatatg aaggcataaa catacttagc
    19081 gataacatca ttactatgac ggcaataatg agtactatgt aaattcttaa tcgcgattta
    19141 gtaattgatt caaattgcgc aatggccaaa aagataaaca tagccgtgat agcaaaatct
    19201 aaccctagcg tttgcggatt tgagatatat tcgccaaata aagccccagc tacacatgaa
    19261 attgcccaaa ataaatatgc tgtgatgtta agaccatgca tccaacgatc attgatagct
    19321 tctcctttta aataaggtgt aatggcgacg ccaaacgttt cgtcagttac taatgaacct
    19381 aatccaacac ggttccaaaa cccatatgtc ttgaagtttg gtgcaagcga catacttaaa
    19441 aggaacattc ttgaatttac gataaataca gttagtacaa tcgctgatat aggtgtacct
    19501 gctataaaca acgcgcacat aataaattgc gcagcaccgg catatataac aagacataac
    19561 aagacaattt ctaaaatact aaagttttga gacgaagcca caataccaaa tgaaatacca
    19621 acaccggcat aacccaataa tgttgggata cactcttgca cgccttgtct aaaacttaaa
    19681 tgtgttgtca tctcaattac ctcctttgcc actttatatt tatataatta cgaaaaaata
    19741 atatacctag tctaatacag ttctataccc taagcaataa cattagacat cagtttgtct
    19801 gaggttagac ataccggagt ctttagtcag cttcatatta actttttatt tttgagaatt
    19861 ttcaattttt tatttaagac tacctccata ttttctatgg atttgtagtt gtttttaagt
    19921 atcaatttta taaattttta tatctgatga tgagtctggg atattgattc atgtaccact
    19981 cccttataat catcccctcc ccctacccta ctccatcgat ataactcata ctacatatca
    20041 acgaaatcag tattttatcg cttcctttcc tatattggtg atgctcaaac ttgttacgtt
    20101 ttagattgtt ttagttcatc ataattatcc cgtattgttg ctataatgaa atgcgttcac
    20161 cccattaaac cacaaactta atttattgtt gttatgtgca ttggctcact attatacttt
    20221 tacagcacaa aaaaagtggc gacagcttcg tcaccacttt ttaaaatatt atttaaagta
    20281 tcttgccctt gctttaagta tacgtagata tatacttttt aaagcttgta gctaaagcct
    20341 ttatttaact ggttttgaaa tttgtgtttt accacccata aatggtacta atgcttctgg
    20401 aattgttact gttccatctt cattttggta attttcaaca atagcagcaa atgtacgtcc
    20461 aactgctaaa ccactaccat ttaatgtatg tgctaattct ggtttagctg ctttgtcacg
    20521 cttgaagcgg atgttagcac gacgcgcttg gaaatccgta cagtttgagc atgaactaat
    20581 ttctttataa tcattgtagc ttggtaacca aacttctaaa tcatatgttt tgcttgcact
    20641 aaatccaata tcacctgtac ataaaataac acgacggtat ggtaaaccta actcttctag
    20701 aattgcttct gcgtttgttg tcatttcttc taaagcattc catgaatctt caggttgttc
    20761 aaaacgtacc atttccactt tatcgaattg atgtaaacga attaatcctc ttgtatctct
    20821 acctgctgat cctgcttcac tacggaaaca tgcagattga ccagtgaatt tttcaggaag
    20881 tacacctggt tgaataattt cattacggta gaaattcgtt aatggtactt cagcagttgg
    20941 aattgtatat aatccttctt tttctacttt aaataaatct tcttcaaatt taggtaattg
    21001 acctgtacca tacattgtat ctgcgttcac aagctgtggt accatcattt ctgtataacc
    21061 atgttgtgtt gtatgttttg taatcatata gttcattaaa gcacgctcta attgcgcacc
    21121 ttcatttgtt aaatatacaa aacgcgcacc tgaaactttt gctgcacgat caaaatcagc
    21181 cattttcaat tcttctacaa tatcccaatg tgctttgggt tcaaatgaaa actcacgtgg
    21241 tgtaccccac tttttaactt caacgttatc ttcatcagat tcaccttgag gtacatcatc
    21301 acttattaaa tttggaatac gacaaaggat acctgtcatt ttattatcaa tttcatttaa
    21361 ttgactatct ttttctttaa tatcgtcacc taatgtgcgc atttcagcaa tcacatcatc
    21421 agcattttct ttattacgtt tttttaatgc gatttcttcg cttactttat tacgacgtgc
    21481 tttcatttct tctgttgcac taattaattt acgtcgttgc tcatccaatt ctaaaatttc
    21541 atctacaact tttggatcat ctccacgtaa ttcaattttg ctcttaactg tgtcaggctc
    21601 atttctgaat aatctaatgt ctaacattaa ccttcatcct ttcccaaata attatcattt
    21661 attatggaat gacgtacgtc tttatttttt agaaaataaa aaaagaccac atccctacaa
    21721 gggacgtggt ctacgcgttg ccaccctatt taacaattta agttataaag atacactaaa
    21781 cctaaattgc acttcactaa aataacggtt atcaccgatt gttcttttaa attaagtagg
    21841 tagattcata tatatgttga ttcttgttca cactaaccac aagctctctg atatcgaaca
    21901 ctatatatta cttgtcctac gaacaatgtc ttattaagtt atttttaata tagcaaacta
    21961 tatttgcttt ttcaagtaac gatttcaaac atcactcatg tcgatttagt gacatgcagt
    22021 cgtttgataa attgattgct ttaaatactg tgcaaccgct tcaatatctt tatgaaattg
    22081 acgatcatgt gtaatggatg gcacgatact tcgaaactca tcatacttgc gacgtgtttt
    22141 tggtgataat ccttcaacac cttttaactc tgctgcttgt aatgcaataa cacattcgat
    22201 tgccagcaca cgtcttgcat tttcaataat ttgataacca tgtctagcag ctgtagttcc
    22261 catagatacg tgatcttctt ggttcgcaga tgaagtgata gaatcaacac tcgctggatg
    22321 cgctaaagtt ttattttcag aaacgagact tgcagcagca tattgcataa tcatcgcgcc
    22381 actttgcaat cctggctctg gactaagaaa tgctggtaaa tcaccattta attgaggatt
    22441 tactagtcgc tctagacgac gttccgatac gtttgctaat tcacttacac ctaatttaag
    22501 atgatctaat gcaaaagcaa taggttgtcc atggaagtta ccacctgaaa taacaaacgt
    22561 ttcatttgct tcctcaaata taagtggatt atcattagcc gcattcattt caaattctaa
    22621 ttgctgttta acataattga atacttgaaa actcgcgcca tggatttgtg gtatacaacg
    22681 caacgtatat gcatcttgta cacgtatttc tgattgtcgc gtcgttaatg ttgatccttc
    22741 taaccaatca cgcatacgcg ctgccacatt aatctgttct tgaaaattac gaactgcgtg
    22801 cacatcatgt cgatatgcat ctataatgcc attaagagac tgatgcgtta atgcagcaat
    22861 ccattcagat tggtaaccta aatcttctgc ttctatataa ctaatgacac cttgagctgt
    22921 catagcttgc gtaccattaa tcaatgctaa accttcttta gcctgaaggt tcaaaggttg
    22981 tctatttaat tctcttaata catcgtcact atccttttct tcccctctgt acaatacttt
    23041 cccttcacca attaatgcta atgctaaatg tgataatggc gctaaatctc ctgatgcacc
    23101 gagagagcct tgctgtggga ttatcggtat aatacgttca tttataaaaa attgtaattg
    23161 tctcactaat tctaaagtgg cacctgaatg accttttaat aatgtattca atcgtaaaat
    23221 catcatgact aatgctactt cttttgaaaa tggctcacct agtccacagg catgtgagcg
    23281 tatcagattc acttgtaatt cattatattg cgtcgggtct atacgtacat cactaaataa
    23341 cccaaaacct gtagtaatac cgtaaaccgt ttcctcattt tcaataatac gttcaactac
    23401 cgctctactt tttttgacac gttctaacgc atcatcaata atttcaatct ttgattgttg
    23461 ttgtaaaaat gatttaatat cctcaattgt tagtgtttca ccatctaaat ataaagtcat
    23521 atatgttacc cccttgttta tattaagtaa cccatccttc ttgaagtata cgttttcatt
    23581 tttattgaaa caatggtttt acgtacattt ataacctatt atcagagcac tattgtagtg
    23641 cgttaaagga tattaagatt gttgtaagca tatttaataa tttatctatt gacgaattgc
    23701 ata
//
LOCUS       DAKTIP010000020        23637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAKTIP010000020 DAKTIP010000000
VERSION     DAKTIP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 23637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.020"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(366..500)
                     /gene="graF"
                     /locus_tag="O0305_000639"
     CDS             complement(366..500)
                     /gene="graF"
                     /locus_tag="O0305_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCY0273795.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            652..1947
                     /locus_tag="O0305_000640"
     CDS             652..1947
                     /locus_tag="O0305_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273796.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            2372..3544
                     /locus_tag="O0305_000641"
     CDS             2372..3544
                     /locus_tag="O0305_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCY0273797.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQQINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSAIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            3598..4140
                     /locus_tag="O0305_000642"
     CDS             3598..4140
                     /locus_tag="O0305_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCY0273798.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISNAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            4294..4560
                     /locus_tag="O0305_000643"
     CDS             4294..4560
                     /locus_tag="O0305_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCY0273799.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            4563..6281
                     /gene="ptsP"
                     /locus_tag="O0305_000644"
     CDS             4563..6281
                     /gene="ptsP"
                     /locus_tag="O0305_000644"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCY0273800.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
     gene            complement(6518..6751)
                     /gene="nrdH"
                     /locus_tag="O0305_000645"
     CDS             complement(6518..6751)
                     /gene="nrdH"
                     /locus_tag="O0305_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCY0273801.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHHIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            6949..8310
                     /locus_tag="O0305_000646"
     CDS             6949..8310
                     /locus_tag="O0305_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCY0273802.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEIGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            8307..9326
                     /locus_tag="O0305_000647"
     CDS             8307..9326
                     /locus_tag="O0305_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCY0273803.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFGISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            9459..10121
                     /locus_tag="O0305_000648"
     CDS             9459..10121
                     /locus_tag="O0305_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCY0273804.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(10470..12167)
                     /locus_tag="O0305_000649"
     CDS             complement(10470..12167)
                     /locus_tag="O0305_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0273805.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(12167..12385)
                     /locus_tag="O0305_000650"
     CDS             complement(12167..12385)
                     /locus_tag="O0305_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCY0273806.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(12414..12536)
                     /locus_tag="O0305_000651"
     CDS             complement(12414..12536)
                     /locus_tag="O0305_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273807.1"
                     /translation="MNDMMNLVINQIVIKLHYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(12866..13417)
                     /locus_tag="O0305_000652"
     CDS             complement(12866..13417)
                     /locus_tag="O0305_000652"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0273808.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            13782..14408
                     /locus_tag="O0305_000653"
     CDS             13782..14408
                     /locus_tag="O0305_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCY0273809.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            14579..15691
                     /gene="pdhA"
                     /locus_tag="O0305_000654"
     CDS             14579..15691
                     /gene="pdhA"
                     /locus_tag="O0305_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCY0273810.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            15695..16672
                     /locus_tag="O0305_000655"
     CDS             15695..16672
                     /locus_tag="O0305_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0273811.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            16763..18055
                     /locus_tag="O0305_000656"
     CDS             16763..18055
                     /locus_tag="O0305_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0273812.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            18059..19465
                     /gene="lpdA"
                     /locus_tag="O0305_000657"
     CDS             18059..19465
                     /gene="lpdA"
                     /locus_tag="O0305_000657"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0273813.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNGIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            19633..19908
                     /locus_tag="O0305_000658"
     CDS             19633..19908
                     /locus_tag="O0305_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCY0273814.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            20052..20591
                     /locus_tag="O0305_000659"
     CDS             20052..20591
                     /locus_tag="O0305_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273815.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            20604..21698
                     /locus_tag="O0305_000660"
     CDS             20604..21698
                     /locus_tag="O0305_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273816.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            21691..22488
                     /locus_tag="O0305_000661"
     CDS             21691..22488
                     /locus_tag="O0305_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273817.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINL
                     LLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
                     KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGN
                     KVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG"
     gene            22494..23303
                     /locus_tag="O0305_000662"
     CDS             22494..23303
                     /locus_tag="O0305_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273818.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            23303..>23637
                     /locus_tag="O0305_000663"
     CDS             23303..>23637
                     /locus_tag="O0305_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0273819.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVP"
BASE COUNT         8205 a         3349 c         4298 g         7785 t
ORIGIN      
        1 tgtcaatagg ttgtcttcat gaaatttcgt ttaattcgat ttaaaattta taatattagc
       61 attggattta aattgaagat gtagtaggaa tgttagtaat taaagatata aaaatatgtg
      121 acatgtaata atattgagct gataaatgaa gagggatact tatcaatcat acctctttaa
      181 caacagtgaa gaacccgtgc ataatggctt acgaattata gtttataagg aagaagaggg
      241 atacatgcgc cgagcacatg cataaaagcc cctaacaact aaaagttgta aggaaggaga
      301 gggatacatg cgccgagcac atgcataaaa tcccctaaca actaaaagtt gtaaggggat
      361 ttaaattaat ttagtgtatc ttggatatct tgttttgttt gattaatatc ttctgttttt
      421 tcttcttttt tatcttttaa tttttcttca acttctttag ctttttctgc tgctttttta
      481 ttttgatttt cattagacat gattaattcc tcccaaattg gataattatt tatatataaa
      541 tcttacccgg ttgtactttc gttaaacttt tctaagtcta tagcactatt tattcattta
      601 tctaaagaca acaacattag attaatatat aatgattttg aggtgaacat aatgtctttt
      661 cttaggaaac acgccgaaat tatttttagc tatttaatcg gtatcgtttc actcttcact
      721 ggtctcatta ttttaattaa cttgccatta attaaacaat taaatggtgg taaaaaagtt
      781 gatacacatg ttcataatgt gtgggaattt ctgaatgcat ttttcagtga aattattaaa
      841 gtaatgagtc gatttatagg taatttccct atagttagtg caattgtgat aattatattc
      901 ggtattttag ttatgttgat tggtcataca ttacttagaa ctattaagta tgactatgat
      961 atttctatct ttttcttagt tatcggtatc atgtacttta ttattactct tatattaatg
     1021 actcaagttt atggattctt tgcagtgatt ttcattattc catttacaat tcatatagga
     1081 tatatcgtct ataaagatga attgaatcag gaaaatgtaa aaaatcattt catgtggata
     1141 attgtgagtt atggtataag ttacttaatt acacaaattg cattgtatgg cagaattgat
     1201 gctaatgaaa tagagtcaat tgatatctta agtgtcaatg ctttctttat aattatgtgg
     1261 ttacttggtc aaatggctat ttggaatttc ttgttcttgc gccgagcttt acctttaaca
     1321 aagcaagaat taggtgaaga ggagccagaa ttatcaagaa caagtaaagg gaatgtcacg
     1381 aatcaaacta aaattcactt gaaacaactc caagataaga ctacagaata tgcacgtaag
     1441 acaagaagaa gtgtcgattt agataaaatt agagctaaaa gagataaatt caaaaagaaa
     1501 gttaatgata ttatcgatat tcaagaagac gatattcctg attggatgag aaaaccgaaa
     1561 tgggttaaac caatgtatgt cgaactattt tgtggtgtcg tcatcttttt attcacattt
     1621 ttagaattta ataatcgtaa tgcattattt gtatctggtg attggaaatt atcacagaca
     1681 caatatgtta ttgaatgggt tacattatta attctgttat tcattattat cgcatatatc
     1741 gctacaacgt taactttcca cttgaaaggt aagttttatt atttacaatt atttatgggg
     1801 agcattttat tctttaaatt gttaacggaa tttataaata taatgattca tggactatta
     1861 ctttcagtgt tcattacgcc aacattacta ttaatgttat tggcaatcat catttcttat
     1921 tcgttacaat tacgagagcg accataatta aaagcattat aaaagtacta tctattaaac
     1981 attttgatgt gtacgctata agttagatat atctctaact tacttagata caggtcaatg
     2041 aagtttatgg atagtacttt ttttgtaact agatttgatt gattcaggtg atgtgaatta
     2101 agtattgaaa attgtataca aagttttagt gcaaataaaa tagttgaaaa gttatccatt
     2161 tgtaaaatca agaaaactag taaatagttg aagcgactta tggaatttgc gaaacgatat
     2221 atagtatttc ctttgtagaa atttaacata tatcattcga attactaatt tgttaaaatc
     2281 aacagtaaga ttagaagtag atgatattga aatttggcaa acaatttaat ctatataaaa
     2341 ctacaacgaa acacagaaag gaagttgtca gatgaaaata gcaactctga acaaaggcaa
     2401 agaaacaaaa tattttaatg gatatccttt aattgaagaa gaggatatct attcacaaga
     2461 tcatttaaaa gaaggagata tttttcaaat tgtgactgat aaatcacaat atgtcgcaac
     2521 ggcttatgtt ggtcgccaac ataaaggatt aggttgggtt ctaacatacg ataaagctca
     2581 acaaatcaac acagctttct ttgtaaaatt gtttaatact gcattagcag aacgtgatta
     2641 ttattttaat atagatggaa caaatgcttt tagattattt aatgctgaag gtgatggtgt
     2701 tgggggatta acaatcgaca attacgatgg tcatttgttg attcaatggt actcaaaagg
     2761 tatttataaa tttaaatatg ccattcttga agcggttaga aaagtatttg attataaatc
     2821 tatttacgaa aaagtaagat ttaaagacag cgaatatagt ggtggttttg ttgaaggaga
     2881 tgcacctgag tttccaattg ttatcgaaga aaacttcaca ttttataatg tagaccttga
     2941 agatggtttg atgacaggta tctttttaga tcaaaaagaa gtgcgcaaga aattaaggga
     3001 tcaatatgcc aaagaacgcc atgttttaaa cttatttagt tatacaggtg ctttttctgc
     3061 aatagcagca agtgaggcat cttcaacaac aagtgtagat ttggctaatc gttctcgtag
     3121 tttaactgaa gaaaattttg gattaaatgc tattgatcct aaatcccaat atatttatgt
     3181 catggacact tttgatttct ataaatatgc tgcacgacat ggacatagtt atgacacgat
     3241 cgtgattgat ccacctagct ttgcgcgtaa caaaaaacgt acattttcag tgcaaaaaga
     3301 ttatgacaaa ttaattaatg gcgccttaaa tatcttatca tctgaaggaa cattattgtt
     3361 atgtacaaac gcaagtgtat atccattaaa gcaatttaaa aatactatta aaaagacgct
     3421 tgaagagagt ggcgttgatt atgaattaac tgaagttatg ggattaccaa aagattttaa
     3481 aacgcatcca cattataagc catctaaata tttaaaagct gtttttgtaa atattagaca
     3541 ttaatttgat aaaaaggggt atagtaactt attgagaaaa agaagggtga taatattatg
     3601 ggattcaaaa acaatttaac atcaaattta acaaataaaa tcggtaattc agtctttaaa
     3661 atagaaaatg ttgacggaaa aggtgcaatg ccaacgacga ttcaagaatt gagagaaaga
     3721 cgacaacgtg ctgaagcaat tgtaaagaga aagtctttaa tgtcatcaac aatgagcgtt
     3781 gttccaattc cgggtttaga ttttggtgtt gatttaaaat taatgaaaga tattatcgaa
     3841 gatgttaata aaatttatgg tttagatcat aagcaagtta atagccttgg ggatgatgtg
     3901 aaagaaagaa ttatgtctgc agcagcaatt caaggtagtc aatttattgg taaaagaatt
     3961 tcaaatgcat ttttaaaaat tgtaattaga gatgtagcta aacgtactgc tgcaaaacaa
     4021 acaaaatggt ttcctgttgt aggacaagct gtgtctgcat ctattagtta ctattttatg
     4081 aataaaattg gaaaagatca cattcaaaaa tgcgaaaatg ttattaaaaa tgtcatgtag
     4141 gtgctataat agttttgcaa tttgcaaatt ttactgaaac cggttttaaa cgaattgaat
     4201 ttaaagcatg gttttggtaa agttaatgta taaaactaag ttagtattgt aataatattg
     4261 aagattctaa ctatacgaag gagaaatgta attatggaac aaaattcata tgtaatcatc
     4321 gacgagactg gtattcacgc tagaccagca acaatgttag tacaaacagc ttcaaaattc
     4381 gattctgata ttcaattaga atataacggt aagaaagtaa acttaaaatc aatcatgggt
     4441 gttatgagcc ttggtgttgg taaagatgct gaaattacaa tttatgctga cggtagtgat
     4501 gaatctgacg ccattcaagc aatcagtgac gtcttatcaa aagaaggatt gactaaataa
     4561 tcatgtctaa attaattaaa ggtattgccg catctgatgg tgtcgcaatt gctaaagctt
     4621 atttattagt tgagccagac ttaacattcg acaaaaatga aaaagtcact gatgttgaag
     4681 gagaagttgc aaagttcaat agcgctatcg aagcttctaa agttgagtta actaaaatta
     4741 gaaataatgc agaggttcaa ctaggtgctg ataaagctgc tatctttgat gcacatttat
     4801 tagttttaga tgaccctgaa ttaattcaac caatccaaga taagattaaa aatgaaaacg
     4861 ctaatgctgc tacagcatta acggatgtaa caacacaatt tgttacaatt tttgaatcta
     4921 tggataacga atacatgaaa gaacgtgcgg ctgatattcg cgacgtttct aaacgtgtgt
     4981 tatcacatat tttaggtgta gaattaccga atccgagtat gattgatgaa agcgttgtta
     5041 ttgtagggaa tgacttaacg ccatctgata ctgctcaatt aaataaagaa ttcgtacaag
     5101 gttttgctac aaacattggc ggaagaacaa gtcactctgc aattatgagt cgttctttag
     5161 aaattccagc aattgttggt acaaaatcaa ttactcaaga agttaaacaa ggcgacatga
     5221 ttatcgtaga tggattaaat ggtgatgtaa tcgttaatcc aactgaagat gagttaatcg
     5281 cttatcaaga taaacgtgag cgttattttg ctgacaagaa agaattacaa aaactacgtg
     5341 atgctgatac tgttacagtt gatggtgttc acgcagagct tgctgcaaat attggtacac
     5401 ctaatgattt gccaggtgtt attgaaaatg gtgcacaagg tatcggctta tatagaactg
     5461 agtttttata tatgggtcgt gaccaaatgc ctacagaaga agaacaattt gaagcttata
     5521 aagaagtatt agaagcaatg ggcggtaaac gtgttgttgt acgtacttta gatataggtg
     5581 gagataaaga attatcatac ttaaacttgc ctgaagaaat gaatccattc ttaggttacc
     5641 gtgcgattcg tttatgcctt gcgcaacaag atattttcag accacagcta cgtgcattat
     5701 tacgtgcatc agtttatggt aagttaaata tcatgttccc aatggttgca acaattaacg
     5761 aatttagaga agctaaagct atattattag aagaaaaaga aaaccttaaa aatgaaggtc
     5821 atgacatttc ggatgatata gaattaggaa tcatggtaga gatacctgca acagcagcat
     5881 tagctgatgt ctttgctaaa gaagtagatt tcttcagtat cggtacaaat gatttaattc
     5941 aatacacatt agctgctgac cgtatgtcag agcgtgtatc atatctatac caaccatata
     6001 acccttcaat cttacgttta gttaaacaag ttattgaagc gtcacataaa gaaggtaaat
     6061 ggacaggtat gtgtggtgaa atggctggag atgaaacagc tattccatta ttgcttggtt
     6121 taggtttaga tgagttctct atgagtgcaa cgtctattct gaaagcaaga agacaaatta
     6181 atggtttaag taaaaatgaa atgactgaac ttgctaaccg tgcagtcgac tgtgcaacgc
     6241 aagaagaagt tattgaatta gttaacaact acgtaaaata attaattctt tcgaaagaca
     6301 gaattataaa taaacccgaa gtattcattg taagtaatta tgaatacttc gggttctttt
     6361 ttatgattta tgttaatttc aatggaatgt tgttgctaac taattaaaca actatgtgtc
     6421 attaacaaaa tgtagttctt taattaatgt tagttaattt aaaaatcgat atttaattta
     6481 tgatagctat aaatgatgaa gttgatttag atgaaaatta atcttggata tttaatactt
     6541 tgttgatttc atcaagatca acatggtaca ttggattgcc attcaacaaa ataaacggag
     6601 ttgaaaaagc atcaaaatct atcatttcgt ttcgatattg ttgattgttg atatttctct
     6661 cttcaaaatc aatgtgatgc tcatttagat aattttttac aaatgtacaa ggtggacaat
     6721 cattctgcgt ataaacgatt atttctgaca tgtatcatgc tccttcgtgc tttattctaa
     6781 tgtaatatac acaattatac attgaaattc aaatttgtga acacattgtg aactgacata
     6841 aattatacac aatttcatag cgcataatgt tttaaaaata cacattatag caattataat
     6901 taagttcatt gtgttgtcat caaaaaaaga aaaaggtgat gtttttaaat ggatacagtt
     6961 gaaatcagtc ggtttttgac agctatgact ttagcagttc atatcatttt tgcaacgatt
     7021 ggtgttggta tgcctttaat gttcgcaatt gcagaatttt taggtattcg caaaaatgat
     7081 cttcaatata tagctatggc caaaagatgg gctaaagctt atacaattac tgtagcagtg
     7141 ggagttgtta caggtacaat tataggactt caattatcat tgatttggcc tacatttatg
     7201 gaaatgggtg gacacgttat tgcacttcca ttatttatgg aaacatttgc gttcttcttt
     7261 gaagctattt tcttaagtat atatttatat acttgggatc gttttaaaaa taaatggaca
     7321 catttcttaa ttagtatacc agtaattatt ggtggctctt tctcagcatt cttcattact
     7381 tcagtgaatt catttatgaa tacgcctgca ggttttgagt tgaagaatgg aaagatggtc
     7441 aatgttcaac ctatagaagc gatgtttaac ccatcgttta tagttcgatc atttcacgta
     7501 attactacag ccggtatgac gatggcattt gttattgcat caatagcagc ttttaaatta
     7561 ttgcgtaatc gtcaacctaa agatactgtc taccataaga aagctttgaa aatgtctatg
     7621 atagttggat tcttttcaac attactttct atgttggcag gggatttatc tgcaaaattt
     7681 ttgcataaat tccaacctga aaaattagca gcttatgaat ggcatttcga tacatcttcc
     7741 catgccaaat tattattatt tggtgtgtta gatgaaaaga ctcagcaagt taaaggtgcg
     7801 attgaattac ctggactatt gagtttctta gcagataata gtgtcaaaac taaagtacaa
     7861 gggttaaatg attttccaaa aagtttacat ccgcctatga tcgtccatta tttctttgat
     7921 ttaatggtaa cgatgggaat tttatgtttt gtcatttcag gtgtttatgt cttaacttta
     7981 atgtttaaaa agcttagaaa gttttctact cataaatgga tgctttatgg aatattatta
     8041 acaggcccag cttcaatgct agctatagaa tttggatggt tcttaacaga gattggtaga
     8101 cagccttgga ttgttcgtgg ttatatgcgc gtggcagaag cagcaacaca agcaggcgga
     8161 ataaccttcg ttacaatttt atttggcata ttgtacatca ttttaatgta tacatgtgca
     8221 tacgtattaa ttcgtatgtt taaaaataaa ccggcgtatg aagatgtaaa tcgtttagcc
     8281 aagaagcaag gaggagaaat agaaaaatga tttatgcatt tataggtata acagtgttat
     8341 ggttgttttt attttgctat atcattattg cttctataga ttttggggca ggtttcttcg
     8401 cattgcattc aaagttaact ggtgatgaaa agaaaattaa tcacttaatt tcacgttatt
     8461 taaacccagt ttgggaagtt acgaatgtat tctttgtttt cttcttcgta ggattcgtag
     8521 gtttctttcc agagtcaatc aaatatttag gcacggtatt gttaatacca ggttcaatag
     8581 cactgattat gatatcgttg agaaacagtt tttatgcatt tgaaaattat ggtcaagata
     8641 caaaattagc atggatgatc atgtatgggg taagtggatt attaattcca gcttcattat
     8701 ctactgcttt aactattaca gaaggtggct atattaatgt tcgaaacaat gttatcgatc
     8761 tagattgggt gcagttatta ttaagcccat ttgcttggtc tgtagtattc ttggcaatta
     8821 tttcagtttt atatatttca tcaggatttt tgacatatta tgctaaaaaa gcaaatgacg
     8881 aaccagcata taatttaaca agacaatggc acatattttt agggccgccg atgattatca
     8941 tttgtttatt cgtatttcta tcattacgca ttcaaaattc tgaacatttt tattcagctg
     9001 tttttgacta ttggtggatg tttggtataa gtttcttatt ctttgcatta gcttcattgt
     9061 taacattctt taagaaaaaa catggtttgg cttttgtatt tgttatttta caaatgatgt
     9121 tcgcgttctt tggctatgga attagtaaat tgccatacct tttatatccg tttgtaaaaa
     9181 ttacagatgc atatgttaat ccagaaatgg gctggacatt agtgattgtc tttattttag
     9241 gtttactttt attacttcca tcgttaatat tattattaag attatttgtt ttcgacaaag
     9301 aatatgttga aggaaagaaa tcataagttg agacgatacc ccaactttca tttacttgca
     9361 ttactgcatt tagataattt agttggggta ctcatttata ataattttaa atttatattt
     9421 tcaatgaatt gatgaaaatc atttaatgga gggcgagtat gggtaaagaa tatgtagtca
     9481 tcggtctagg tcgttttgga ggtagtatcg ttcgtgagtt gaatgcatta gacatggatg
     9541 taatggccat cgaccatgat gaaaatagag tgaatgaata tagtgatatc gctactcatg
     9601 cggttgttgc agacactaca gatgaagcag ttatgaaaag tttaggtatc cgtaactttg
     9661 atcatgtcat tgtggcaatt ggtgaaaata ttcaatcaag tacgttgacg actttaattt
     9721 taaaagagtt aggtgtaaaa aaagtaactg ctaaagcaca aaatgattat catgcaaaga
     9781 ttttaaataa aattggagca gatacggttg tgcaccctga gcgtgatatg ggtagacgta
     9841 ttgcgcataa tgttgcgagt gcaagtgtac ttgattatct tgagttggca gacgagcatt
     9901 ctattgtaga attgaaagca actgaaaaga tggcggggca gtctatcatt gatttagata
     9961 taagagcaca atatggaatt aacattattg caattaaacg aggcaaagag ttcatcattt
    10021 caccaaatcc aaatattaat ttagaaatag gtgatatttt aatcatgatt ggacatgata
    10081 atgatttaaa tcgctttgaa aaaaatattg cgacgagata atcgggagtg ggacagaaat
    10141 gatattttct caaaatttat ttcgtcgtcc caccccaact tacattgtct gtagaaattg
    10201 ggaatccaat ttctctttgt tggggcccac accccaactc gcattgcctg tagaatttct
    10261 tttcgaaatt ctctatgttg gggcccctga ctagaattga aaaaagcttg ttacaagcgc
    10321 attttcgttc agtcaactac tgccaatata actttgtaga gcattgaaca ttgatttatg
    10381 tctcaagctc atcatatata aagaaaaagc tactaacaat gggtggtgag tttaattaaa
    10441 acttcacttc aaagttagta gctttttgat tatttattgt ttgattcttt ttgttcgttt
    10501 accttcataa tgactggtaa aatcattggt tttctagctg ttttttcaaa taaataaggt
    10561 tgtaatgttt caatgataga agatttaatc tgatgccatt gaatatcttt attttgattt
    10621 aacttactaa taacatcagt tttgattttg cgttgtgcat cataaattaa ttgacctgat
    10681 tccctcatat atacaaatcc tcgagaaata atgtctggac cagaaagtaa tttatttgta
    10741 ttaaaatcaa tactaacaac aacgataact aaaccttctt cagataatag cttacggtct
    10801 cttattacaa cattaccgat atcaccgata ccactaccat caacaagtac attaccagat
    10861 ggaatgcgac cagctttacg tgctgaatcg tgtgttaaag ctaagacatc tccaatatca
    10921 aagatgaaga cattatcttc ttcaacgccg cattcaacac cagtctcacc atgtgctttt
    10981 aacatacggt attcaccatg aataggtaag aaatatttcg gcttgattaa tcgaagcatt
    11041 aattgttgat caccttgaga accatgccct gaagtatgga tgttagaaat cttgctatgg
    11101 ataacatctg caccagcttt atacaaggaa ttaatagttc tgttaatact ttttgtatta
    11161 cctgggatag gtgatgaact aaatacaacg gtatcttcag gtataatttt aatttgctta
    11221 tgagtaccat tagcaattct agataatgct gccattggtt caccttgtga accagtacat
    11281 agtatcaata actcatgctt cggtacggta ttaattttat taggttcaat aaatgtttca
    11341 ggtggtgctt taatataacc aagttccata cctattttaa tattgttttc catcgaacga
    11401 ccgaacgtaa caattttacg gttatttttg atagcagctt caactgcttg ttgaactcgg
    11461 taaatattag aagcgaaggt agcaaatata atacgacctt tacaattacg gaagatctta
    11521 tctacgtttt gaccaacttc acgttcgctt aaagtaaaat caggcacaag tgaatttgtt
    11581 gagtctgaaa gtaaacatag aacgccttct tcgcctaatt gagccatttt agcaatgttt
    11641 gctggtttgc ctacaggtgt aaaatcaaat ttaaagtcac cggtatgaac tacttttcct
    11701 tcaggtgtat ctacgatgac gccataagtt tcaggaatac tatgtgtagt taagtagaaa
    11761 gaaatcgtaa agtgcttaga tttaatcaca ctgtcctcat tgatttcatt tagtttagca
    11821 gtacgtaata aatgatgttc ttcaagttta ttacggatta aacctaatgc taaaggacca
    11881 ccataaatag gtatattaag ttgttttaat aggaagggca caccgcctat atggtcttcg
    11941 tgaccatgtg ttataaatag gccaacaatt ttatcttggt tttgaactag atatgtgtag
    12001 tcaggtataa cataatcaat ccctaataag ttatcatcag ggaatttgat acctgcatcg
    12061 ataatgacaa tttcgtcttt atactcaact gcataagtat ttttaccgat ttcacctaga
    12121 cctccaagtg catatacacc tacttcattt ggatgtaatt gtttcattat ttagcaatct
    12181 ccacattaaa gtgttctgag ttttcttttt cgtaatctaa atgtgcgccc tctaatttag
    12241 tgataaattc gatattaaaa ttacgatctt tcaagtaacg acgtacttgt tcttctgttt
    12301 gagcttcaac ataaagtgat tgtgtatttt cacgcacaat tacctcgtct ctgttatgtt
    12361 gataaaaaac tttaaatact gccatgttaa aaattcctcc taagaatgtt tgtttaattt
    12421 attgttaacc ctagtaaaat cgtattggag tatatatcga taaattcatt ccaatcatct
    12481 ctataatgta acttaataac gatttggtta ataactaggt tcatcatgtc gttcatttta
    12541 aaaaattagt gaaataacac taaaatttca gttaaatctt attttacatg atgaatgata
    12601 ataaataaag cagtttatct cactagaaat ctaagtattt cgaataaatg tgcagttcta
    12661 tttattaaaa taaatgtaga gattataaat taatgttaag aaagaattaa aggattttat
    12721 atgaaacggt aaaatttaaa tgaggggata aaaagtttgg tagaaaggtg aatatattta
    12781 actgttgttt attcgaaaaa gttgtaaaag gaaaagctta ggacatcata tttatcctaa
    12841 gctttgataa ttagaaaaat gtgttttaaa cttctactgc atctgtatgt ggttgtaatg
    12901 ggtgatcttt gtcaatgtga tcatagaaca ttacaccatt taaatggtca atttcatgtt
    12961 ggaaaacaat tgctggatat ccttttagtc gtaattgtat atcattacct tcgatgtctt
    13021 tggctttaat tgtaattcta ttatgacggt gaactagacc agcaacatta tcatcgacac
    13081 taaggcaacc ttcaccagtt ggtaaataag cttcttgaac gctatgactt acaatttttg
    13141 ggttcacaag catatagtca taagatttgc cactgccatc atctggtatt aaaacagcaa
    13201 tcatacgttt agaaatatta atttgaggtg cagccaaacc aacgcctgaa cgtaaaccat
    13261 atcgtttcgc gatttcctca tcttgactat ttactaaaaa ctctctcatg gcgattaatg
    13321 tttctttttc ttctttagtt aatggtaatt ctaactcagc tgctttttga cgcaaagttg
    13381 gatgaccatc tctaatgatg tctttcattg ttaacatata ttgcaccttc cttattttaa
    13441 tttgttttag ttgaatgaca gtaaaaaggt tgttaagata ctcatacatt tttatgtgta
    13501 aatatctaca aagttaacca actactgcca atgtttattt tagatagtat atgtaaattt
    13561 tcagatatgc taattgctta aaaaatgatt aaagtgttgg tttcaagcaa tgatacttta
    13621 gaaatttatt tatcatcttg actttaaaaa ttatattata aatgacgtaa ctgtcaacag
    13681 atatacttag taatgaagat gtgtaatgta attgtttaaa attgatttcc aagcagattt
    13741 tatttatcat ttaatttaaa tagcaagtgg aggtacaagt aatgaaattt ggaaaaacaa
    13801 tcgcagtagt attagcatct agtgtcttgc ttgcaggatg tactacggat aaaaaagaaa
    13861 ttaaggcata tttaaagcaa gtggataaaa ttaaagatga tgaagaacca attaaaactg
    13921 ttggtaagaa aattgctgaa ttagatgaga aaaagaaaaa attaactgaa gatgtcaata
    13981 gtaaagatac agcagttcgc ggtaaagcag taaaggattt aattaaaaat gccgatgatc
    14041 gtctaaagga atttgaaaaa gaagaagacg caattaagaa gtctgaacaa gactttaaga
    14101 aagcaaaaag tcacgttgat aacattgata atgatgttaa acgtaaagaa gtaaaacaat
    14161 tagatgatgt attaaaagaa aaatataagt tacacagtga ttacgcgaaa gcatataaaa
    14221 aggctgtaaa ctcagagaaa acattattta aatatttaaa tcaaaatgac gcgacacaac
    14281 aaggtgttaa cgaaaaatca aaagcaatag aacagaacta taaaaagtta aaagaagtat
    14341 cagataagta tacaaaagta ctaaataagg ttggtaaaga aaagcaagac gttgatcaat
    14401 ttaaataatt aatataatac agatggtagg aaacaactaa tacagttcct attatctgta
    14461 tcttttttta ttaaaacaga actttttcaa atggtttaac agtcccattt atttgtggta
    14521 caattagtaa ggataaaatg aatttctata caattatggg aaaggtatgg tgaattgaat
    14581 ggctcctaag ttacaagccc aattcgatgc agtaaaagtt ttaaatgata ctcaatcgaa
    14641 atttgaaatg gttcaaattt tggatgagaa tggtaacgtc gtaaatgaag acttagtacc
    14701 tgatcttacg gatgaacaat tagtggaatt aatggaaaga atggtatgga ctcgtatcct
    14761 tgatcaacgt tctatctcat taaacagaca aggacgttta ggtttctatg caccaactgc
    14821 tggtcaagaa gcatcacaat tagcgtcaca atacgcttta gaaaaagaag attacatttt
    14881 accgggatac agagatgttc ctcaaattat ttggcatggt ttaccattaa ctgaagcttt
    14941 cttattctca agaggtcact tcaaaggaaa tcaattccct gaaggcgtta atgcattaag
    15001 cccacaaatt attatcggtg cacaatacat tcaagctgct ggtgttgcat ttgcacttaa
    15061 aaaacgtggt aaaaatgcag ttgcaatcac ttacactggt gacggtggtt cttcacaagg
    15121 tgatttctac gaaggtatta actttgcagc agcttataaa gcacctgcaa ttttcgttat
    15181 tcaaaacaat aactatgcaa tttcaacacc aagaagcaag caaactgctg ctgaaacatt
    15241 agctcaaaaa gcaattgctg taggtattcc tggtatccaa gttgatggta tggatgcgtt
    15301 agctgtatat caagcaacta aagaagcacg tgaccgcgca gttgcaggtg aaggtccaac
    15361 attaattgaa actatgacat atcgttatgg tcctcataca atggctggtg acgatccaac
    15421 tcgttacaga acttcagacg aagatgctga atgggagaaa aaagacccat tagtacgttt
    15481 ccgtaaattc cttgaaaaca aaggtttatg gaatgaagac aaagaaaatg aagttattga
    15541 acgtgcaaaa gctgatatta aagcagcaat taaagaggct gataacactg aaaaacaaac
    15601 tgttacttct ctaatggaaa ttatgtatga agatatgcct caaaacttag cagaacaata
    15661 tgaaatttac aaagagaagg agtcgaagta agccatggca caaatgacaa tggttcaagc
    15721 gattaatgat gcgcttaaaa ctgaacttaa aaatgaccaa gatgttttaa tttttggtga
    15781 agacgttggt gttaacggcg gtgttttccg tgttactgaa ggactacaaa aagaatttgg
    15841 tgaagataga gtattcgata cacctttagc tgaatcaggt attggtggtt tagcgatggg
    15901 tcttgcagtt gaaggattcc gtccggttat ggaagtacaa ttcttaggtt tcgtattcga
    15961 agtatttgat gcgattgctg gacaaattgc acgtactcgt ttccgttcag gcggtactaa
    16021 aactgcacct gtaacaattc gtagcccatt tggtggtggc gtacacacac cagaattaca
    16081 cgcagataac ttagaaggta ttttagctca atctccaggt ctaaaggttg ttattccttc
    16141 aggcccatac gatgcgaaag gtttattaat ttcttctatt agaagtaatg acccagtcgt
    16201 atacttagag catatgaaat tgtatcgttc attccgtgaa gaagtacctg aagaagaata
    16261 tacaattgac attggtaagg ctaatgtgaa aaaagaaggt aatgacattt caatcatcac
    16321 atacggtgcg atggttcaag aatcaatgaa agctgcagaa gaacttgaaa aagatggtta
    16381 ttctgttgaa gtaattgact tacgtactgt tcaaccaatc gatgttgaca caattgtagc
    16441 ttcagttgaa aaaactggtc gtgcagttgt agttcaagaa gcacaacgtc aagctggtgt
    16501 tggtgcagca gttgtagctg aattaagtga acgtgcaatc ctttcattag aagcacctat
    16561 tggaagagtt gcagcagcag atacaattta tccattcact caagctgaaa atgtttggtt
    16621 accaaacaaa aatgacatca tcgaaaaagc aaaagaaact ttagaatttt aatacatttt
    16681 aaaagttaac gaagttagcg tattttagtc tcattgatta aaatgaaatg tttaatttac
    16741 gaaatcttag gagggcaaaa acgtggcatt tgaatttaga ttacccgata tcggggaagg
    16801 tatccacgaa ggtgaaattg taaaatggtt tgttaaagct ggagatacta ttgaagaaga
    16861 cgatgtttta gctgaggtac aaaacgataa atcagtagta gaaatcccat caccagtatc
    16921 tggtactgta gaagaagtta tggtagaaga aggtacagta gctgtagttg gtgacgttat
    16981 tgttaaaatc gatgcacctg atgcagaaga tatgcaattt aaaggtcatg atgatgattc
    17041 atcatctaaa gaagaacctg cgaaagagga agcgccagca gagcaagcac ctgtagctac
    17101 tcaaactgaa gaagtagatg aaaacagaac tgttaaagca atgccttcag tacgtaaata
    17161 cgcacgtgaa aaaggtgtta acattaaagc agtttctgga tctggtaaaa atggtcgtat
    17221 tacaaaagaa gatgtagatg catacttaaa tggtggtgca ccaacagctt caaatgaatc
    17281 agctgcttca gctacaagtg aagaagttgc tgaaactcct gcagcacctg cagcagtaac
    17341 attagaaggc gacttcccag aaacaactga aaaaatccct gctatgcgta gagcaattgc
    17401 gaaagcaatg gttaactcta agcatactgc acctcatgta acattaatgg atgaaattga
    17461 tgttcaagca ttatgggatc accgtaagaa atttaaagaa atcgcagctg aacaaggtac
    17521 taagttaaca ttcttacctt atgttgttaa agcacttgtt tctgcattga aaaaataccc
    17581 agcacttaac acttcattca atgaagaagc tggtgaaatc gttcataaac attactggaa
    17641 tatcggtatt gcagcagaca ctgatagagg attattagta cctgttgtta aacatgctga
    17701 tcgtaagtct attttccaaa tttcagatga aattaatgaa ttagctgtta aagcacgtga
    17761 tggtaaatta acagccgatg aaatgaaagg tgctacatgc acaatcagta atatcggttc
    17821 agctggtgga caatggttca ctccagttat caatcaccca gaagtagcaa tcttaggaat
    17881 tggccgtatt gctcaaaaac ctatcgttaa agatggagaa attgttgcag caccagtatt
    17941 agcattatca ttaagctttg accacagaca aattgatggt gcaactggcc aaaatgcaat
    18001 gaatcacatt aaacgtttat taaataatcc agaattatta ttaatggagg ggtaaaacat
    18061 ggtagttgga gatttcccaa ttgaaacaga tactatagta atcggagcag gtcctggtgg
    18121 atacgttgca gcaattcgtg cagctcaatt aggacaaaaa gtaacaatcg ttgagaaagg
    18181 taatcttggt ggtgtttgct taaacgtagg atgtattcct tcaaaagcat tactacatgc
    18241 ttctcaccgt tttgttgaag cacaacattc tgaaaactta ggtgttattg ctgaaagtgt
    18301 ttctttaaac ttccaaaaag ttcaagaatt caaatcatca gttgttaata aattaactgg
    18361 tggtgttgaa ggcttactta aaggtaacaa agttaacatc gttaaaggtg aagcatattt
    18421 cgtagataac aatagcttac gtgttatgga cgaaaagagc gcacaaacat acaactttaa
    18481 aaatggaatc attgcaacag gttcaagacc aattgaaatt cctaatttca aattcggtaa
    18541 acgtgttatc gactcaacag gtgctttaaa cttacaagaa gtaccaggta aattagttgt
    18601 agttggtgga ggatacattg gatcagaatt aggtacagca tttgctaact ttggttcaga
    18661 agtaaccatc cttgaaggtg ctaaagatat cttaggtggc ttcgaaaaac aaatgacaca
    18721 acctgttaaa aaaggtatga aagaaaaagg tgttgaaatc gttactgaag ctatggctaa
    18781 atcagctgaa gaaacagata acggagttaa agttacttat gaagctaaag gcgaagagaa
    18841 aacaatcgaa gctgattatg tattagtaac tgtaggtcgt cgtccaaaca cagacgaatt
    18901 aggcctagaa gaattaggtg ttaaattcgc tgaccgtgga ttattagaag ttgataaaca
    18961 aagccgtacg tctatcagca atatctatgc aattggtgat atcgttccag gtttaccact
    19021 tgctcacaaa gctagctatg aagctaaagt tgctgctgaa gcaattgatg gtcaagctgc
    19081 tgaagttgat tacattggta tgccagcagt atgctttact gaaccagaat tagctacagt
    19141 tggttattca gaagcgcaag ctaaagaaga aggtttagca attaaagctt ctaaattccc
    19201 atatgcagca aatggtcgtg cattatcatt agatgatact aacggatttg ttaaacttat
    19261 tacacttaaa gaagatgata ctttaatcgg tgctcaagta gttggtactg gtgcatcaga
    19321 tattatctct gaattaggtt tagcaattga agctggtatg aatgctgaag atatcgcatt
    19381 aacaatccat gcacatccaa cattaggtga gatgactatg gaagcagcag aaaaagctat
    19441 cggataccca atccatacaa tgtaataact gattatctat aaagattcag tcattaaaag
    19501 ctgtagcata tgctacggct tttttgtttt aggtaaagta atgtaaggaa attgatttga
    19561 gatatcgtta acatgtgaca tgcatgttat actagcgatg ctaacaaaag aattgaaatg
    19621 gagggttcaa caatggaata tgagtatcca attgatttag actggagtaa tgaagagatg
    19681 atttcagtga taaatttctt taatcatgta gagaagtatt atgaatccgg cgtgacggca
    19741 ggcgacttta tgggtgcata taaaagattt aaagaaattg tgcctgctaa agcagaggaa
    19801 aaacaaattt ttaatacttt cgaaaaaagt agtggctata atagttacaa agcagttcaa
    19861 gatgtaaaaa ctcactctga agaacaaaga gtaacagcta aaaaataatt cgttcgaaat
    19921 taacacaatt taataggaat ttttctttaa aactattgct aataaagcta tattttgata
    19981 cctttatcaa gtgttaaaca aaatgtttga taaaagtaaa cttaatatag cttttttagg
    20041 tggaaaaata aatgaacata ggtaataaaa ttaaaaatct tagaagaatt aaaaatttaa
    20101 cgcaagaaga acttgctgaa cgtacagact tatcgaaagg ctacatttca caaatagaaa
    20161 gtgaacatgc ctcaccaagt atggaaactt tcttaaatat tatagaggtg ttaggaacga
    20221 cgccaagtga attttttaaa gacagtgaaa atgaaaaagt attatacaag aaggaagaac
    20281 aagttattta tgatgagtat gatgaaggtt atatattaaa ttggttagtt tcaaagtcaa
    20341 atgaatatga tatggagcca ttaatattaa ctttaaagcc tggagcatca tataaaaatt
    20401 ttaatccatc agagtctgat acgtttattt attgtatgtc aggtcagata acacttaatt
    20461 taggcaaaga gatatatcaa gcacaagaag aagacgtttt gtattttaaa gcacgagata
    20521 atcatcgttt gtcaaacgaa tcaaacaatg aaacacgaat acttattgta gcgacagctt
    20581 catatttata ggggggatct tatttggaac cgttattatc attaaaatca gttagtaaaa
    20641 gctatgatga tcttaatatc ttagatgaca tagatattga tattgaatca ggatactttt
    20701 atacattatt aggtccttca ggttgtggta aaacaacaat tttaaaatta attgcagggt
    20761 ttgaatatcc tgacagtggt gaagtgattt atcaaaacaa accaattggt aatttaccac
    20821 caaataaacg taaagtgaat acagtctttc aagattatgc attatttcca cacttaaacg
    20881 tctatgataa tatcgctttt ggtttgaaat taaaaaaatt atcaaaaacc gaaattgatc
    20941 aaaaagtaac tgaggcatta aaattagtaa aactttcagg ttatgaaaaa agaaatatta
    21001 atgaaatgag tggcggacaa aagcaacgtg ttgcaattgc acgtgctatc gtaaatgaac
    21061 cagaaatatt attgttagat gaatctttat ccgcattaga tttgaaattg cgtactgaaa
    21121 tgcaatatga attacgagaa ttgcaatcta gattaggtat tacatttata tttgtaacac
    21181 atgatcaaga agaagcgtta gcattaagtg actttctttt tgtattaaaa gatgggaaaa
    21241 ttcaacaatt tggcacacca acagatatat atgacgaacc agtgaatcga tttgtagctg
    21301 attttattgg agaatctaat attgttgaag ggcgcatggt tagagattat gtcgtgaata
    21361 tttatgggca agatttcgaa tgtgtcgata tgggtattcc tgaaaataaa aaagtagaag
    21421 tcgttattcg accagaagat atatcattaa tcaaagctga agaaggatta tttaaagcaa
    21481 ctgttgattc tatgttattt agaggggtcc actatgaaat atgttgtata gacaataaag
    21541 gttatgaatg ggtaatacaa acgactaaaa aagctgaagt aggcagtgaa gttggtcttt
    21601 attttgatcc tgaagccatt catattatgg ttcctggaga aacagaagaa gaatttgata
    21661 aacgtattga aagctatgag gaagtagaca atgcgtaata ctaataaatt tctcttaatc
    21721 ccgtatttac tatggatggt tatatttatt attgtaccag ttgtattact catttatttt
    21781 tcatttttag atatcaatgg acattttagt ttcacgaatt atcaacaaat ttttactaca
    21841 aaatatttga aaatgtttgc atattcaatt ttatatgccg cttcaataac aattattacc
    21901 ttgactatca gttatccagc tgcctattat attactcgtt cgaaatttca aaatatctta
    21961 ttaatgataa tgattattcc aacatggata aatttattgt taaagacata tgcttttata
    22021 ggtttattaa gtcatgatgg cgtgattaat caatttttcc acttatttaa tttaccatca
    22081 ttcaatttgt tatttacaac tggtgcgttt ttagtagtgg caagttacat ttatatacca
    22141 tttatgattt tacctatatt taatagcatg aaagcaattc ctaataattt attgcaggcc
    22201 tcaagtgatt taggtgccag tcctttctat acttttagaa aagtaatcat gccgttaaca
    22261 aaagaaggtg ttatgactgg gattcaagta acctttattc catcactttc attatttatg
    22321 attacaagat taattgcagg taataaagtc ataaatatag gtacggcaat agaggaacaa
    22381 tttttaacaa ttcaaaatta tggtatggga tcaactatag ctatattctt aattgtattt
    22441 atggcattca ttttaatcat tacaaaatca tctaatggga gagggtgaat catatgaaat
    22501 ggtatggaaa gctgtatatc gggatacttt tagcgatttt atacatccca atattctttt
    22561 taatgttcta ttcattcaat tcggctggta atatgattca ctttgaacat tttacattag
    22621 agcattatca atcattattt caaaatgatc atttaatgtc ggtcattttt aatacgatag
    22681 ctgtagcact tttagcagcc tcaatttcta cagttattgg tacatttggt gccattgcta
    22741 tttattattt aagaaataaa aagtttaaag taactttact aacattgaat aatgtcttga
    22801 tggtatcatc cgacgttgtc ataggtgcat cattcttaat tatgtttaca acgattggcc
    22861 attttactgg tcttggttta ggattttgga cagttctaat atctcatatt gcattttgca
    22921 tacctatagt tgtgattatc gtcttaccac aactgtatga aatgaataat aatatgttaa
    22981 atgctgcaag agatttagga gcgactgaac cacaattatt aagcaacatt attattccta
    23041 atattttacc ttctattata ggaggattct ttatggcttt aacttattca ctagacgatt
    23101 tcacagtaag tttcttcgtt actggtaatg gctttagtgt gttatcagtt gaagtttatg
    23161 ctatggcgag aaaaggaatt agtatggaaa ttaatgcgat ttcaacatta ttgtttgctg
    23221 ttattgtatt aggaatacta ggatattatt tgattcaata cgtgataaat aagaaaaaac
    23281 taatcaagcg aggtgtaaaa taatgaaacg atttttacaa ctcattatag gtgcattagt
    23341 tgtgggtatg ctttgtctta ctttaagtca ttggtttaaa tctaaagaac aagtgcatac
    23401 aaatcaaaaa atttacgtat acaattgggg cgaatatatt gatccagagt taattaagaa
    23461 atttgaaaaa gaaactggca ttcaagtcgt ttatgaaact ttcgattcaa atgaagcgat
    23521 ggaagccaaa attcgcaatg gcggtacaca ttatgatgtt gcttttccta gtgaatatac
    23581 agttcaaaaa ttgaaaagag atcatttatt gttaccaata gatcataata aggtacc
//
LOCUS       DAKTIP010000021        22978 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAKTIP010000021 DAKTIP010000000
VERSION     DAKTIP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 22978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22978
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.021"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(157..1155)
                     /gene="sirC"
                     /locus_tag="O0305_000664"
     CDS             complement(157..1155)
                     /gene="sirC"
                     /locus_tag="O0305_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCY0273820.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1152..2147)
                     /gene="sirB"
                     /locus_tag="O0305_000665"
     CDS             complement(1152..2147)
                     /gene="sirB"
                     /locus_tag="O0305_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCY0273821.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2163..3155)
                     /gene="sirA"
                     /locus_tag="O0305_000666"
     CDS             complement(2163..3155)
                     /gene="sirA"
                     /locus_tag="O0305_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCY0273822.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3386..4366
                     /gene="sbnA"
                     /locus_tag="O0305_000667"
     CDS             3386..4366
                     /gene="sbnA"
                     /locus_tag="O0305_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCY0273823.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4363..5373
                     /gene="sbnB"
                     /locus_tag="O0305_000668"
     CDS             4363..5373
                     /gene="sbnB"
                     /locus_tag="O0305_000668"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCY0273824.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5394..7148
                     /gene="sbnC"
                     /locus_tag="O0305_000669"
     CDS             5394..7148
                     /gene="sbnC"
                     /locus_tag="O0305_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCY0273825.1"
                     /translation="MQNHTAVNTAQAIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRVSKQPLTAAEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTVPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7141..8397
                     /gene="sbnD"
                     /locus_tag="O0305_000670"
     CDS             7141..8397
                     /gene="sbnD"
                     /locus_tag="O0305_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCY0273826.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMPKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVVGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            8387..10123
                     /gene="sbnE"
                     /locus_tag="O0305_000671"
     CDS             8387..10123
                     /gene="sbnE"
                     /locus_tag="O0305_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCY0273827.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSE
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPTHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDSVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10032..11882
                     /gene="sbnF"
                     /locus_tag="O0305_000672"
     CDS             10032..11882
                     /gene="sbnF"
                     /locus_tag="O0305_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCY0273828.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11857..12633
                     /gene="sbnG"
                     /locus_tag="O0305_000673"
     CDS             11857..12633
                     /gene="sbnG"
                     /locus_tag="O0305_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCY0273829.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12633..13835
                     /gene="sbnH"
                     /locus_tag="O0305_000674"
     CDS             12633..13835
                     /gene="sbnH"
                     /locus_tag="O0305_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCY0273830.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERKILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTNQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFDIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13839..14603
                     /gene="sbnI"
                     /locus_tag="O0305_000675"
     CDS             13839..14603
                     /gene="sbnI"
                     /locus_tag="O0305_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCY0273831.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14799..15206
                     /locus_tag="O0305_000676"
     CDS             14799..15206
                     /locus_tag="O0305_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273832.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15256..15882
                     /locus_tag="O0305_000677"
     CDS             15256..15882
                     /locus_tag="O0305_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273833.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELDSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            16094..16870
                     /locus_tag="O0305_000678"
     CDS             16094..16870
                     /locus_tag="O0305_000678"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCY0273834.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17014..17136
                     /locus_tag="O0305_000679"
     CDS             17014..17136
                     /locus_tag="O0305_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273835.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            17206..18186
                     /locus_tag="O0305_000680"
     CDS             17206..18186
                     /locus_tag="O0305_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0273836.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNNHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            18149..18841
                     /locus_tag="O0305_000681"
     CDS             18149..18841
                     /locus_tag="O0305_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCY0273837.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19051..20217
                     /locus_tag="O0305_000682"
     CDS             19051..20217
                     /locus_tag="O0305_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCY0273838.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHNDVYVIVGNHGPMIE
                     QLDARVNVIIIEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRIVFTAHGWAFTEGVKPAKKFLYLVIEKLMSLITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENEVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20198..21436
                     /locus_tag="O0305_000683"
     CDS             20198..21436
                     /locus_tag="O0305_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0273839.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMSVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21426..22856
                     /locus_tag="O0305_000684"
     CDS             21426..22856
                     /locus_tag="O0305_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCY0273840.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         7664 a         3562 c         4458 g         7294 t
ORIGIN      
        1 ggtgttattt gtatataaag tattaatatg taattaagaa agtcgacgat tgttaacaat
       61 aacaacaact atatgtataa aaaaacctcg caacggttag ttaactgaat catttagcta
      121 acttcgttgt gaggtcattt tgttttaata atatcgttat aactttttca cggttaataa
      181 taagtatatg aagaatgggg caccaaaagc agcaataaat acacctgctg gcacttcttt
      241 aggcaagaat aaggtacgcc caattaagtc tgcaataaca attgatatgg caccaatcat
      301 tgctgacatt agtaactttt tagcataact tccgcgaacg attgttttcg cgatatgtgg
      361 tgcgattaaa ccgacaaacc caatgttacc tactaaactg attgccatag atacgagtat
      421 agtagaagtg attaattgga ttagtttcat acgttgtaca tgtaagccta agccaatcgc
      481 tacagggtca tcaagtatag atattttcat ttttggtata acaagaaata acaacggcac
      541 aacagctaaa ataaccatac ccaaaatgat tgtatcttta aacgtagcac cgtaaagact
      601 tccgactagc catgtataag ctttggcagc agataattgc ttcgttgtaa tgagtaatcc
      661 ttggacaagc gcaataaaca acgtttgcat cgaaataccg atgattatga gtgttgtcgg
      721 gcgtatttgt cctttcgttt gaaacactaa tagtatcatc attgcaactg cgccacctaa
      781 tactgcaaat agtggaagta aatgtattgt taaatggctg aaaaatgcaa taaagacaac
      841 agcacttaag ctagcaccac ctgtgatacc gataatatca ggtgaggcaa ttggattttt
      901 taatacattt tgcaacatta aaccactcat tcctagtgcg gcacctgcta aaatcgcaag
      961 tgtaatgcga ggtaagcgta atacttctaa agtgaattga tccatactgt catttggatt
     1021 tataaagtac atcagtacgc gttgtaatgg tataaagctt gaaccaatca tcatacttac
     1081 cactgaaacg atggctaaaa agattaacgc gaagatgaga tggtaattgt cttttttatt
     1141 aatcttttcg gtcataagcg ttgacgtcct ttcttcataa tatagattaa gacaatagcg
     1201 ccaatgacag cggtaacgac accgataggc aactctagtg gcttaattat tatacgagca
     1261 acaatgtctg aaatgatcat taggattgct ccagctaatg cagtaaaagg aattaaatac
     1321 ttatagtttg gtggtaataa tcgtttgcta atattcggta cgataagacc cacaaagacg
     1381 attgatccag ctacggctac cgaaataccg gctaacatac tgatgagcat aataatcatc
     1441 catttgatta attttatgtt ttgaccgagg ccggttgcaa tgtcgtcact tgtcatcaag
     1501 atgttgatgt gtgcagccat gctaaatgca attaaaataa gtatcaatac aagcggaata
     1561 atccatggga tatcccaaat attacgtaat gaaacggagc cacttaacca aaataatagg
     1621 ccttgtaagt ctgtttcgtt cataataagt atgccttgag taaaggctgt aaatagcatc
     1681 gcaatcgcag cacctgccaa aatgacacgg tgaggtgaga atagtgtttg tctaaacata
     1741 cctagtgcaa caactaatac agtaacaaca atagccccca aaaatgcaat aactacaatc
     1801 attttaaaag attgaatttg gataaatgta atactaaaaa tgacaaaaaa tactgcgcct
     1861 gcattgacac cgaaaagccc tggtgaggct attgggtttc gtgtaagtgc ttgcatcaac
     1921 aaacctgaga cagcaagggc agcaccagtc aataacgcaa tgattgttct cgacgcccgt
     1981 gcaccagtga caacatcatg taaatcgttt tcactatcaa agttgaataa cgcctgtatc
     2041 accgtacctg gtgacacaag cgtatttcca atcattaaac ttaagatagc tactattgca
     2101 agacataaac cagcaataac gatttggtat tttggtttaa gtagcatcgt aaaactcctt
     2161 aattattttg attgtttttc aatatttaac ttttcatata aatcgtcaat aagttttaat
     2221 gaagatttat atccgccagc taagttccaa gtgatttcat ctaaatcatc agatacttgg
     2281 ttgtttttaa ctgcgtctaa atttttccac tctttacttg aagtccattc gctttcagtc
     2341 tttttaacta atgcagcatc tttcgcattt ggatctgatt ttactacaaa aatatgatca
     2401 gcgttcatta atggaatgct ttctttagat gtaagttgga taatatcttt accattatca
     2461 acttgttttt gtaagtcttt attacgtttg aatcctaaat catttaagat ttcaccagca
     2521 tatccaccag cataaattct tgtatgatca gcacggaagt taacaactga agctttcaat
     2581 ggccatgcat ctttatactt tgcttttgca tctttttgga atgcagctac tttatcatcg
     2641 tactttttaa gtaaatcttc agcttctttt tctttcccta aagctttccc cattaactta
     2701 gttgtatctt tgaatttgaa aactgtatca gtagaaactg ttggtgcgat tttagataat
     2761 tgatcgtaaa ctttttcatt tctaactttt gacgcgacaa ttaagtccgg ttttaattta
     2821 gagatttcct ctaagttagg tgcaggttct tgacctacaa tcttagtatc ttttaaatca
     2881 ttttttatgt attcgaattt cggtttttgt gtccatgatt ctacagcacc tacaggttta
     2941 acacctaaag atacagcgac gtcagtggca ccttgatata gcgtaacaac acgctttggt
     3001 ttccctttaa tttcagttgt acccattgca tgtttaattg aagttgtttc cttatctttg
     3061 ttatcagatg attgtttatt tgaattccca ctacatcctg ctaaaacaag taggaaagca
     3121 agcgtaacaa caagcatttt aattacttta ttcattgact aattagcctc cttcgtgatg
     3181 tatgacaatg agaatcatta tcacgattta gtatgaatta aattttttcc taagtcaata
     3241 aaatatttat gatttacatg caacttataa ttatttgaca tataaatgca taaaaaatat
     3301 aatcctaatt acttgatagt gagaatcatt atcaattagg taacacacaa tattatagaa
     3361 ttttaaattt gaggaggaag cgcttttgat tgaaaaaagt caagcatgtc acgattcatt
     3421 gttagattct gtagggcaaa cacctatggt tcaacttcat caactatttc cgaaacatga
     3481 agtgtttgca aagttagagt atatgaatcc tggaggcagc atgaaagatc gacctgccaa
     3541 gtacatcatt gaacatggta ttaaacatgg tttaatcact gagaatacac atttaattga
     3601 aagtacttct ggtaatttag gcattgcgtt ggcaatgata gctaaaatca agggattaaa
     3661 actcacgtgt gttgttgatc ctaaaatatc accaacaaat ttgaaaatta ttaaaagtta
     3721 tggtgccaat gtagaaatgg ttgaagaacc tgatgcacat gggggttatt taatgactcg
     3781 tattgcaaag gtgcaagaac tgttagccac tattgacgat gcatattgga ttaatcaata
     3841 tgcgaatgag ttaaattggc aatcccatta tcatggtgca ggcacagaga ttgttgaaac
     3901 aattaagcaa cctatagatt attttgtcgc gccagtcagc acgacaggta gcattatggg
     3961 tatgagtaga aaaataaaag aagtgcatcc aaacgcacaa attgttgctg ttgatgcgaa
     4021 agggtcagtc atttttggtg acaaacctat taatagagaa ttacctggta tcggtgctag
     4081 tcgtgtaccc gaaatattga atagatcaga aattaatcaa gtgatccatg tagatgatta
     4141 tcaatctgct ttgggctgtc gaaaactgat tgattatgaa ggcatatttg ccggaggttc
     4201 aacaggttcg attattgcag cgattgagca gttgataacg tcaattgaag aaggtgcaac
     4261 aattgtcacg attttaccag atcgaggcga tcgttactta gatttagttt attcagatac
     4321 atggttagaa aaaatgaaat caagacaagg agttaaatca gaatgaatag agagatgttg
     4381 tatttaaata gatcagatat tgaacaagcg ggaggtaatc attcacaagt ttatgtggac
     4441 gcattaacag aagcattaac agcccatgcg cacaatgatt ttgtacaacc gcttaagccg
     4501 tatttaagac aggatcctga aaatggacac atcgcagatc gaattattgc aatgccaagt
     4561 catatcggtg gtgaacacgc aatttcaggt attaagtgga taggtagtaa gcacgacaat
     4621 ccatcgaaac gtaatatgga gcgtgcaagt ggcgtcatta ttttgaatga tccagaaacg
     4681 aattatccaa ttgcagttat ggaagcaagt ttaattagta gtatgcgtac tgcagcagtt
     4741 tcagtgattg cagcaaagca tttggctaaa aaaggattta aagacttaac aatcattgga
     4801 tgcgggctaa tcggagacaa gcaattacaa agtatgttag agcaattcga tcatattgaa
     4861 cgcgtgtttg tttacgatca attctctgaa gcatgtgcac gctttgttga tagatggcaa
     4921 caacagcgtc cggaaattaa ttttattgcg acagaaaatg ctaaagaagc agtatcaaat
     4981 ggtgaagtag tcattacatg taccgtaacg gatcaaccat acattgaata tgattggtta
     5041 caaaagggtg catttattag caacatttct atcatggatg tgcataaaga agtctttatt
     5101 aaagctgaca aagtcgtagt agatgactgg tcacaatgta atcgagaaaa gaaaactatt
     5161 aaccaattgg tgttagaagg taaattcagc aaagaagctc ttcatgctga actaggacaa
     5221 cttgtgacag gtgacatacc aggacgtgaa gacgatgatg agatcatatt acttaatccg
     5281 atgggtatgg ctatcgaaga tatttcaagt gcttatttta tttatcaaca ggcacaacaa
     5341 caaaatattg ggacaacatt gaacctatat taagaatgcg aggtgtctga acattgcaga
     5401 atcatacagc agtcaataca gcacaagcga taatattaag agatttagtt gatgcattat
     5461 tatttgaaga tatagccgga attgtatcga atagtgagat tactaaagaa aacggacaaa
     5521 cgcttttgat atacgaacgt gaaacacaac aaataaagat acctgtttat tttagtgctt
     5581 taaatatgtt tcgttacgaa agttcacaac caattacgat agagggaagg gtgtctaagc
     5641 aacctttaac ggcagctgaa ttttggcaaa caattgctaa tatgaattgt gatctaagtc
     5701 atgaatggga agtggctcgc gttgaagaag gactgactac tgctgccaca cagcttgcta
     5761 aacaattatc agaattagat ttagcgtcac atccttttgt gatgtcagag cagtttgcaa
     5821 gtttaaaaga tcgtccattt catccattag ctaaagaaaa aagaggatta agagaagcgg
     5881 attatcaagt gtatcaagct gaattaaatc aatcatttcc tttaatggtt gcagcagtta
     5941 aaaagacaca tatgattcat ggcgatactg caaatatcga tgaattagaa aatttgacag
     6001 tacctataaa agaacaagcg acagacatgt taaatgatca agggttatca atagatgact
     6061 atgtactatt tccggtacat ccttggcaat atcagcatat tctgccgaac gtctttgcga
     6121 aagagattag tgaaaagttg gttgtactat taccgttaaa atttggagat tatctgtcgt
     6181 cttcaagtat gcgttcatta attgatattg gcgcaccgta taaccatgtc aaagtaccat
     6241 ttgcaatgca gtcattaggc gcattaaggc taacgcctac gcgttacatg aaaaacggag
     6301 aacaagcaga acaattatta cgtcagctta tagaaaaaga tgaagcacta gctaagtatg
     6361 tcatggtttg tgatgaaaca gcttggtggt catatatggg tcaagataat gatattttca
     6421 aagatcaatt aggtcatcta actgttcagc taagaaagta tcccgaagtg ctagccaaaa
     6481 atgatacgca acagctagtg tcaatggcag cactcgcggc aaatgatcgc actttatatc
     6541 aaatgatttg tggaaaagat aatatttcta aaaatgatgt catgacgtta tttgaagata
     6601 tcgcgcaagt ctttttaaag gtaacactat catttatgca atacggcgca ttaccagagt
     6661 tgcatggtca aaatatattg ttgtcatttg aagatggacg tgtacaaaaa tgcgtgttac
     6721 gtgatcatga tactgtcaga atttataaac catggctaac agcacatcag ctttcattgc
     6781 cgaagtatgt cgtcagagaa gatacaccta atacgctaat taatgaggat ttggaaacat
     6841 tctttgctta ttttcaaaca ttagctgtat cggtaaatct atatgccatt attgatgcaa
     6901 ttcaagattt atttggtgta agtgagcatg aacttatgtc gttgttaaaa caaattttaa
     6961 aaaatgaagt ggcaactatt tcctgggtta caactgatca gctagctgtc agacacattt
     7021 tatttgataa acagacgtgg ccattcaaac aaattttatt accattgcta tatcaacgtg
     7081 atagtggtgg aggtagtatg ccttcaggtt taactaccgt accaaatcca atggtgacat
     7141 atgattaatc agtctatatg gcgcagtaac tttcgcattt tatggctcag tcagtttata
     7201 gcgattgctg gactgacagt acttgtgcca ttattgccaa tttatatggc atcactacaa
     7261 aatctatcag tcgtagaaat acagttgtgg agtggtatag cgattgctgc tccagctgta
     7321 acgacgatga tagcttcgcc gatatggggg aagctaggtg ataagatcag ccgaaaatgg
     7381 atggtgttaa gagcgttact tggtttggcg gtatgcttat ttttaatggc attgtgtacg
     7441 acaccattac agtttgtact tgtgaggtta ttgcagggac tatttggtgg tgttgttgat
     7501 gcatcaagtg cgtttgcgag tgcagaggcg ccagctgaag atcgtggaaa ggtattagga
     7561 agactgcaaa gttcagtcag cgcagggtct cttgtggggc cattaattgg cggtgttaca
     7621 gcttcgatat taggttttag tgcgttactg atgagtattg ccgttattac ttttattgtc
     7681 tgtattttcg gtgcattaaa attgattgaa acgacacata tgccaaaatc acaaacacca
     7741 aatattaata aaggtattcg ccgttcattt caatgtctat tatgcacaca acaaacatgt
     7801 cgatttatta tcgttggcgt tttagcaaac tttgctatgt atggcatgct aactgcatta
     7861 tcaccacttg cttcatcagt gaatcataca gcgatagatg accgtagtgt gattggattt
     7921 ttacagtccg cattttggac ggcttcgata ttaagcgcgc ctttatgggg acgctttaat
     7981 gataaatcat atgttaaatc agtatatata tttgccacga ttgcatgtgg ttgtagtgcg
     8041 atactgcaag gtttagcgac gaatatagag tttttaatgg ctgcaagaat acttcaagga
     8101 ttaacatata gtgcattgat tcaaagtgtc atgtttgttg tcgtgaatgc gtgtcatcaa
     8161 caacttaaag gcacatttgt tggaacgacg aacagtatgt tagttgttgg tcaaattatt
     8221 ggcagtctta gtggcgctgc cattacaagt tatactacac cagctactac gtttatcgtt
     8281 atgggcgtag tatttgcagt aagtagttta tttttaattt gttcaaccat cactaatcaa
     8341 atcaacgatc acacattaat gaaattatgg gagttgaaac aaaaaagtgc aaaataaaga
     8401 attaatacaa catgcagcgt atgcggctat cgaacgcatt ttaaatgaat attttagaga
     8461 agaaaattta tatcaagtac cacctcaaaa tcatcaatgg tctatacaat tatcagagct
     8521 cgaaacttta acgggtgaat ttcgctattg gtctgcgatg gggcatcata tgtatcatcc
     8581 agaggtatgg cttatcgatg gaaaaagtaa aaaaataaca acttataaag aagcaattgc
     8641 gcgtattttg caacatatgg ctcaaagtgc agataatcaa acggcagtgc aacaacatat
     8701 ggcgcaaatt atgtctgaca tcgataatag cattcatcgc acggcacgtt atttgcaaag
     8761 taacacaata gactacgtag aggatcgtta tatcgtttca gaacaatctt tatacttagg
     8821 tcatccattt catccgactc ctaagagtgc aagtgggttt tcagaagcag atttagagaa
     8881 atatgcaccc gaatgtcata catcattcca attgcattat ttagctgtgc atcaagatgt
     8941 tctgctcacg cgctatgtag aaggtaaaga agatcaggtt gagaaagtgt tgtatcaatt
     9001 agcagacata gatatatcag agatacccaa agattttatt ttattaccaa cacatcctta
     9061 tcaaatcaat gtgttgcgac agcatccaca gtatatgcaa tatagtgaac aaggtttaat
     9121 aaaagacctt ggcgtttccg gtgattcagt gtacccgacg tcttcggtta gaactgtatt
     9181 ttcaaaagca ttaaacattt atttaaaatt accgatacac gttaaaatca ctaattttat
     9241 acgtacgaat gaccttgaac agattgaacg gacaattgat gccgcgcaag ttatcgcatc
     9301 agtcaaagat gaggttgaaa caccccattt taaattgatg tttgaagaag gatatcgtgc
     9361 attgttaccg aatccattag ggcaaacagt tgaacctgaa atggatttat taacaaatag
     9421 tgccatgatt gttcgtgaag ggataccgaa ttaccatgct gataaagata ttcatgtatt
     9481 ggcgtcatta tttgaaacga tgcctgattc accgatgtct aagttatcac aagtgattga
     9541 gcaaagtggt ttagcaccag aagcatggct tgaatgttat ttgaatcgta cattattgcc
     9601 gatattaaag ctgtttagta acacaggcat tagtctagaa gcacatgtac aaaatacatt
     9661 aattgaatta aaagatggca tacccgacgt atgctttgtc agagatcttg aaggcatttg
     9721 tctatctaga acgattgcta ctgaaaaaca gcttgtgcca aatgttgtgg cagcatcaag
     9781 ccctgttgta tatgcacatg atgaagcatg gcatcgtctt aaatattacg ttgtagtaaa
     9841 tcacttagga catttagtat caactattgg taaagcgact agaaatgaag ttgtgttatg
     9901 gcaacttgta gcgcatcgtc ttatgacttg gaaaaaagaa tacgcgaata acgcagtatt
     9961 tgttgactgt gtagaagatt tatatcaaac gccgaccatt gcggctaaag cgaatttgat
    10021 gagtaaattg aatgattgtg gtgcaaaccc tatttataca catataccaa atccaatttg
    10081 tcataacaag gaggtatcgt attgtgaatc aaacaattct taatcgtgta aagactagag
    10141 tgatgcacca actggtatca tcacttattt atgagaatat tgttgtgtat aaagcgtcat
    10201 atcaagacgg tgtcggtcat tttacaatag aaggacatga ttcagagtat cgttttactg
    10261 ctgaaaagac acatagcttt gatcgtatac gtatcacatc accaattgag cgtgtcgtag
    10321 gagatgaggc agatacaaca acagactata cacaattatt gagagaggtt gtatttacat
    10381 ttcctaaaaa tgatgaaaag ctagaacaat ttattgtcga gttattacag acagaattaa
    10441 aagatacgca aagtatgcag tatcgagaat caaacccacc agcaacacct gagacattta
    10501 acgactatga attttatgcg atggaagggc atcagtatca tccaagttac aaatcacgtt
    10561 taggatttac gttgagtgat aatttgaaat ttggtcctga ttttgtacca aacgttaaac
    10621 tgcagtggtt agctatcgac aaagataaag tagaaacgac ggtatcaaga aatgttgtag
    10681 ttaacgaaat gttacgtcaa caagttggcg ataagactta tgaacatttt gtacagcaga
    10741 ttgaagcatc tggcaaacat gtaaatgatg ttgagatgat acctgtacac ccatggcagt
    10801 ttgaacatgt catccaagtt gatttggctg aagaaaggct taatggcaca gtactatggt
    10861 taggggaaag tgatgagcta tatcaccctc aacaatcgat tcgtacgatg tcgccaatag
    10921 acacgacaaa atattattta aaggtaccaa taagtataac gaacacttca acgaaacgag
    10981 tgttggcgcc tcatacaatt gaaaatgcag cgcaaattac ggattggtta aagcagatac
    11041 agcaacaaga tatgtattta aaagatgaat taaagacagt ttttctaggg gaagtcttag
    11101 gacagtctta tttaaataca caactttcgc cttataaaca aactcaagtt tatggtgcgt
    11161 taggtgttat atggcgtgaa aatatatatc atatgttaat cgatgaagag gatgcgatac
    11221 catttaatgc actttatgca agtgataagg atggtgtacc attcattgaa aattggatta
    11281 aacaatatgg ttctgaagct tggacaaagc aatttttagc tgtagcgatt cgtccaatga
    11341 ttcatatgct ttattatcac ggtattgcct ttgaatcgca tgcacaaaat atgatgctca
    11401 ttcatgaaaa tggttggcct acacgtattg ccttaaaaga tttccatgat ggtgttcgtt
    11461 ttaagcgtga gcatttaagt gaagctgctt cacacctgac attaaagcca atgccagaag
    11521 cacataaaaa agtgaatagt aattcattta ttgaaacaga tgacgaacgt ttagtacgcg
    11581 actttttaca tgatgcattt ttctttatta atatcgccga aatcatctta tttattgaaa
    11641 agcaatatgg tatcgatgag gagctgcaat ggcaatgggt taaaggcatc atcgaggcgt
    11701 atcaagaagc atttccagag ttgaataact atcaacattt cgatttgttt gaacctacga
    11761 ttcaagttga aaagttaacg acacgtcgat tattaagtga ctccgagtta agaattcatc
    11821 atgttacaaa tccattaggt gtaggaggta tcaatgatgc aacaactatc tctgaaacat
    11881 agattaaaca atggtgattc agtttatggc atttttaatt ctataccgga cccattgatg
    11941 atcgaggtta tcgcagcaag cgggtatgac tttgttgtga ttgatacaga acacgtggcg
    12001 attaatgatg agacactagc gcatttaatt cgtgcagctg aagcagcgca tattatacca
    12061 attgtacgtg tcactgcagt gatagataga gatatcatta aagtgttgga tatgggtgcg
    12121 agaggtatta ttgtgccaca cgttaaagat cgtgagacag ttgagcatat tgtgaaatta
    12181 agtcgttatt acccgcaagg attaagaagt ttgaatggtg gtcgcatggc aagatttgga
    12241 cgtacaccat tacttgatgc aatggagatg gctaatgagc atattatggt gattgccatg
    12301 atagaagatg ttgaaggggt tatggccatt gacgatatag cacaagtcga aggtttagac
    12361 atgatagtcg aaggtgccgc agatttatcg cagtcacttg gcataccatg gcaaacgcgt
    12421 gatgatcaag taacatcaca tgttcaacat atttttgaag ttgtgaatgc acatggtaaa
    12481 catttttgtg cattaccacg tgaagatgaa gatattgcaa aatggcaggc acaaggtgta
    12541 caaacattta ttttaggtga tgatcgcgga aaaatatatc gccatttaag tgcatctcta
    12601 gcgacgtcta aacagaaagg ggatgaaggc taatgcgtat agttcaacct gttattgaac
    12661 aattaaaagc acaatctcat ccagtttgtc attatatcta tgatttagtc ggactggaac
    12721 atcatttgca acatattaca tcgtcattgc cgagtaattg tcaaatgtac tatgcaatga
    12781 aagcaaatag tgaacgaaaa atcctagata caattagtca gtatgttgaa ggattcgaag
    12841 ttgcatctca aggtgaaata gcaaaaggtc ttgcttttaa accagcaaat catattattt
    12901 ttggtggccc tggtaagaca gacgaggaac taagatatgc agtaagtgaa ggtgttcagc
    12961 gtattcatgt tgaaagtatg catgaattac aacggctaaa tgccatctta gaagatgaag
    13021 ataagacaca acacatttta ttgcgtgtta atttagcagg accatttccc aatgcaacgt
    13081 tgcatatggc aggacgccca acacaatttg gtatttctga agacgaagtt gatgatgtca
    13141 ttgaagctgc gctcgcaatg ccaaagattc atctagatgg atttcatttt cattctattt
    13201 ctaacaattt agactcgaat ttacatgtcg atgtagtgaa actttatttt aaaaaagcaa
    13261 aggcatggtc tgaaaaacat cgatttccac tcaaacatat caatcttggt ggtggcatag
    13321 gcgttaacta tgcagattta actaaccaat ttgaatggga taattttgta gaacgtttta
    13381 aaacacttat cgttgagcaa gaaatggaag atgtgacatt gaactttgaa tgtgggcgct
    13441 ttattgtggc acatattggt tactatgtga cagaagtgct agacattaag aaagtacatg
    13501 gtgcttggta tgccatatta agaggaggta cgcaacaatt tagactgccg gtatcttggc
    13561 aacataacca tccttttgac atttatcgct ataaggacaa tccatattca tttgaaaaag
    13621 tttcaatttc gagacaggac acaacgttag tcggtcaatt atgtacaccg aaagatgtct
    13681 ttgctagaga agtacagata gacgcaatca gtacaggcga cgttattgtt ttcaaatatg
    13741 caggtgcata cggatggtct atttcacatc acgatttctt aagccatcca catcctgaat
    13801 ttatttattt aacgcaaaca aaggaggatg aataactatt gaatcatatt catgaacatt
    13861 taaaattggt accagtagat aagattgatc ttcacgaaac attcgaacct ttaagattgg
    13921 aaaaaacgaa aagtagtatt gaagcagatg attttatacg tcatcctatt ttagtgacag
    13981 cgatgcaaca tggtagatat atggttatag atggtgtgca tcggtataca agtttgaaag
    14041 cgttaggatg taagaaagtt ccagtgcaag aaatccatga aacacaatat tcaattagta
    14101 catggcaaca taaagttcca tttggtgtgt ggtgggaaac gttacaacaa gaacatcgct
    14161 tgccatggac tactgagaca agacaagaag cgccatttat tacaatgtgt catggtgata
    14221 cagaacaata tttgtataca aaagatttag gcgaagcaca ttttcaagta tgggaaaagg
    14281 ttgtcgcaag ttatagtggt tgttgttctg tagagagaat tgcacaaggt acatatcctt
    14341 gtctttctca acaagatgta ctcatgaagt atcagccatt gagttataag gaaattgaag
    14401 cggttgttca taaaggggaa actgtgccag caggtgtgac acgctttaat atttcaggac
    14461 gatgtcttaa tcttcaagta ccactggcat tacttaaaca agatgatgat gttgaacaac
    14521 tgcgcaattg gaagcagttt ttagcagata agtttgccaa tatgagatgc tatactgaaa
    14581 aagtatactt ggtggagcaa tagttttact gtgatgttga gggaaatatg atgatttagc
    14641 gtattgatag cgaaaatata ataaaacaat atagtgtgga gaacttttga tattttataa
    14701 atattgaagt tctccatttt tgtattttgc atataaaaat taaataaaat aaggtatatt
    14761 aaggtaaagt ataaatttta aataaatggg gaatgagtat gagctcaatt ataggaaaaa
    14821 tagcaatttg gataggcatc gtagctcaaa tatattttag tgtcgttttt gttaggatga
    14881 tatctattaa tattgctgga ggatctgatt acgaaacaat ttttttatta ggattaatat
    14941 tggctctttt cactgtttta ccaaccatct ttactgcgat ttatatggaa agttactctg
    15001 taatcggagg tgcacttttt attgtttatg ctattattgc actgtgttta tataatttcc
    15061 tttcgtcaat tttatggctg attggtggta ttttgctgat ttggaataaa tactcaaaag
    15121 atgaatcgac agacgaaaat gaaaaagttg atattgaaag tacagagaat caatttgaat
    15181 ctaaagataa aatcactaaa gaataaagag aatatttaag gtaaagtata aattttaaat
    15241 aaatggggaa tagacatgga aaaaaatgta gaaaaatcat tcataaagat aggtttatat
    15301 tttcaaatag cttatatagt actcatggct ataactttat gtgggtttgt aatttgctat
    15361 ggactaattt tcggcctttt ctatttatta tcaggtagca gagctgatta tttaatagta
    15421 acaatagtta tatcggcaat aatttctata tttgtaatta tactttcaat cgtacctgtc
    15481 atcgtattgg catctgactt atttaaagaa aggatttcaa aaggtgtcat attaattgta
    15541 ttggctatta tcgctttagt attatgcaac tttgtatctg caatactctg gtttgtttca
    15601 gccatatcta ttttaggtag aaaaaaatta gtagctgcag cagatactac cactattcaa
    15661 aaaagtaaag ggaacgcaaa tcaagcatca cataaagaca cgtgtaaaaa ggaacttgat
    15721 agtcaagaca tgatggaaca tcctgaggtt aaaaatccca cgactaaaaa ccttgaagga
    15781 tttaacgaag aaatacataa agatgaagct acaactaaag ttgtcagtga taacacggaa
    15841 ccgcctattg aatcaaaaga ccatgtctcg aaaaaagatt gatgacaaac taatcgagag
    15901 acttaaaaaa ataatattca acataagaac ttttaaaacg acatttaaac gcattgccaa
    15961 tcactaatgg tagtgcgttt aactatacct taaatatctg aatattttgt taaatggagc
    16021 tacctttgtt gtactattca aatgaagagg agtaaaatgt aattaaagga aagaaatttg
    16081 aggagtgatc tttatgacaa acaacaaagt agcattagta actggcggag cacaagggat
    16141 tggttttaaa attgcagaac gtttagtgga agatggtttc aaagtagcag ttgttgattt
    16201 caatgaagaa ggggcaaaag cagctgcact taaattatca agtgatggta caaaagctat
    16261 tgctatcaaa gcagatgtat caaaccgtga tgatgtattt aacgcagtaa gacaaactgc
    16321 cgcgcaattt ggcgatttcc atgtcatggt taacaatgcc ggccttggac caacaacacc
    16381 aatcgataca attactgaag aacagtttaa aacagtatat ggcgtgaacg ttgcaggtgt
    16441 gctatggggt attcaagccg cacatgaaca atttaaaaaa ttcaatcatg gcggtaaaat
    16501 tatcaatgca acatctcaag caggcgttga gggtaaccca ggcttgtctt tatattgcag
    16561 tacaaaattc gcagtgcgag gtttaacaca agtagccgca caagatttag cgtctgaagg
    16621 tattactgtg aatgcattcg cacctggtat cgttcaaaca ccaatgatgg aaagtatcgc
    16681 agtggcaaca gccgaagaag caggtaaacc tgaagcatgg ggttgggaac aatttacaag
    16741 tcagattgct ttgggcagag tttctcaacc agaagatgtt tcaaatgtag tgagcttctt
    16801 agctggtaaa gactctgatt acattactgg acaaacaatt attgtagatg gtggtatgag
    16861 attccgttaa taatcatcca ctaatgataa ataaatcctt attgttaagt ttaatcactt
    16921 agcagtaagg attttttagt gcacttagaa gggagtgtat tggtagaaaa ttaataagcg
    16981 aagttcttaa gtgagttatg atgtcacagt ctaatgcatc agttgaaagc attattagta
    17041 ttaacacacc caagatatta taaaacatca caaaaacacc actatctaat ttatctcaat
    17101 aaaaattcac aaagttatct cattttattt ttataaataa aaaatatcga taaaaagctt
    17161 acaatacttt atgtttttat gatatatttt taatgtataa atgaggtgga agatttggaa
    17221 agagttttga taactggtgg ggctggtttt attgggtcgc atttagtaga tgatttacaa
    17281 caagattatg atgtttatgt tctagataac tatagaacag gtaaacgaga aaatattaaa
    17341 agtttggctg acgatcatgt gtttgaatta gatattcgtg aatatgatgc agttgaacaa
    17401 atcatgaaga catatcaatt tgattatgtt attcatttag cagcattagt tagtgttgct
    17461 gagtcggttg agaaacctat cttatctcaa gaaataaacg tcgtagcaac attaagattg
    17521 ttagaaatca ttaaaaaata taataatcat ataaaacgtt ttatctttgc ttcgtcagca
    17581 gctgtttatg gtgatcttcc tgatttgcct aaaagtgatc aatcattaat cttaccatta
    17641 tcaccatatg caatagataa atattacggc gaacggacga cattaaatta ttgttcgtta
    17701 tataacatac caacagcggt tgttaaattt tttaatgtat ttgggccaag acaggatcct
    17761 aagtcacaat attcaggtgt gatttcaaag atgttcgatt catttgagca taacaagcca
    17821 tttacatttt ttggtgacgg actgcaaact agagattttg tatatgtata tgatgttgtt
    17881 caatctgtac gcttaattat ggaacacaaa gatgcaattg gacacggtta taacattggt
    17941 acaggcactt ttactaattt attagaggtt tatcgtatta ttggtgaatt atatggaaaa
    18001 tcagtcgagc atgaatttaa agaagcacga aaaggagata ttaagcattc ttatgcagat
    18061 atttctaact taaaggcatt aggatttgtt cctaaatata cagtagaaac aggtttaaag
    18121 gattacttta attttgaggt agataatatt gaagaagtta cagctaaaga agtggaaatg
    18181 tcgtgaaaat gacattgaag ctgtccataa taataagggt tatgcctatc aaagaaaatt
    18241 agacaaacta gaagaagtga gaaaaagcta ttacccaatt aaacgtgcga ttgacttaat
    18301 tttaagcatt gttttattat ttttaacttt accgattatg gttatattcg ccattgctat
    18361 cgtcatagat tcgccaggaa accctattta tagtcaggtt agagttggga agatgggtaa
    18421 attaattaaa atatacaaat tacgttcgat gtgcaaaaac gcagagaaaa acggtgcgca
    18481 atgggctgat aaagatgatg atcgtataac aaatgtcggg aagtttattc gtaaaacacg
    18541 cattgatgaa ttaccacaac taattaatgt tgttaaaggg gaaatgagtt ttattggacc
    18601 acgcccggaa cgtccggaat ttgtagaatt atttagttca gaagtgatag gtttcgagca
    18661 aagatgtctt gttacaccag ggttaacagg acttgcgcaa attcaaggtg gatatgactt
    18721 aacaccgcaa caaaaactga aatatgacat gaaatatata cataaaggta gtttaatgat
    18781 ggaactatat atatcaatta gaacattgat ggttgttatt acaggggaag gctcaaggta
    18841 gtcttaattt acttaataag ttcaaataaa agttatattt taaagattgt gaccaattgt
    18901 tacagtataa cgaggaatcc cttgagacag tatcaaatgg cattaagaaa tatgtgccat
    18961 cattgatttg catggctata aatactattc atctgatgag atagccatgt taagaaattg
    19021 aaagtatagc attaaagggg tttgtaacag ttgaaaatta tatattgtat tactaaagca
    19081 gacaatggtg gtgcacaaac acatctcatt caactcgcca accatttttg cgtacacaat
    19141 gatgtttatg tcattgtagg caatcatgga ccaatgattg aacaactaga tgcaagagtt
    19201 aatgtaatta ttatcgaaca tttagtaggt ccaattgact ttaaacaaga tattttagct
    19261 gtcaaagtgt tagcacagtt attctcgaaa attaaacctg atgttatcca tttacattct
    19321 tccaaagctg gaacggtcgg acgaattgcg aagttcattt cgaaatcgaa agacacacgt
    19381 atagttttta ctgcacatgg atgggctttt acagagggtg ttaaaccagc taaaaaattt
    19441 ctatatttag ttatcgaaaa attaatgtca cttattacag atagcattat ttgtgtttca
    19501 gatttcgata aacagttagc gttaaaatat cgatttaatc gattgaaatt aaccacaata
    19561 cataatggta ttgcagatgt tcccgctgtt aagcaaacgc taaaaagcca atcacataac
    19621 aatattggcg aagtagttgg aatgttgcct aataaacaag atttacagat taatgccccg
    19681 acaaagcatc aatttgttat gattgcaaga tttgcttatc caaaattgcc acaaaatcta
    19741 atcgcggcaa tagagatatt gaaattacat aacagtaatc atgcgcattt tacatttata
    19801 ggcgatggac ctacattaaa tgattgtcag caacaagttg tacaagctgg gttagaaaat
    19861 gaagtcacat ttttgggcaa tgtcattaat gcgagtcatt tattatcaca atacgatacg
    19921 tttattttaa taagtaagca tgaaggtttg ccaattagca ttatagaagc tatggctaca
    19981 ggtttgcctg ttatagccag tcatgttggc ggtatttcag aattagtagc tgataatggt
    20041 atatgtatga tgaacaacca acccgaaact attgctaaag tcctggaaaa atatttaata
    20101 gacagtgatt acatcaaaat gagtaatcaa tctagaaaac gttatttaga atgttttact
    20161 gaggagaaaa tgattaaaga agtggaagac gtttataatg gaaaatcaac acaatagtaa
    20221 attactaaca ttgttactta tcggtttagc ggtttttatt cagcaatctt cggttattgc
    20281 cggtgtgaat gtttctatag ctgactttat cacattacta atattagttt atttactgtt
    20341 tttcgctaac catttattaa aggcaaatca ttttttacag tttttcatta ttttgtatac
    20401 atatcgtatg attattacgc tttgtttgct attttttgat gatttgatat ttattacggt
    20461 taaggaagtt cttgcatcta cagttaaata tgcatttgta gtcatttatt tctatttagg
    20521 gatgatcatc tttaagttag gtaatagcaa aaaagtgatc gttacctctt atattataag
    20581 cagtgtgact ataggtctat tttgtattat agctggtttg aacaagtccc ctttactaat
    20641 gaaattgtta tattttgatg aaatacgttc aaaaggatta atgaatgacc ctaactattt
    20701 cgcgatgaca cagattatta cattggtact tgcttacaag tatattcata attacatatt
    20761 caaggtcctt gcatgtggta ttttgctatg gtctttaact acaacggggt ctaagactgc
    20821 gtttatcata ttaatcgtct tagccattta tttctttatt aaaaagttat ttagtagaaa
    20881 tgcggtaagt gttgtgagta tgtcagtgat tatgctgata ttactttgtt ttacctttta
    20941 taatatcaac tactatttat tccaattaag cgaccttgat gccttaccgt cattagatcg
    21001 aatggcgtct atttttgaag agggctttgc atcattaaat gatagtgggt ctgagcgaag
    21061 tgttgtatgg ataaatgcca tttcagtaat taaatataca ctaggttttg gtgtcggatt
    21121 agtggattat gtacatattg gctcgcaaat taatggtatt ttacttgttg cccataatac
    21181 atatttgcag atctttgcgg aatggggcat tttattcggt gcattattta tcatatttat
    21241 gctttattta ctgtttgaat tatttagatt taacatttct gggaaaaatg taacagcaat
    21301 tgttgtaatg ttgacgatgc tgatttactt tttaacagta tcatttaata actcaagata
    21361 tgtcgctttt attttaggaa ttatcgtctt tattgttcaa tatgaaaaga tggaaaggga
    21421 tcgtaatgaa gagtgattca ctaaaagaaa atattattta tcaagggcta taccaattga
    21481 ttagaacgat gacaccactg attacaatac ccattatttc acgtgcattt ggtcccagtg
    21541 gtgtgggtat tgtttcattt tctttcaata tcgtgcaata ctttttgatg attgcaagtg
    21601 ttggcgttca gttatatttt aatagagtta tcgcgaagtc cgttaacgac aaacggcaat
    21661 tgtcacagca gttttgggat atctttgtca gtaaattatt tttagcgtta acagtttttg
    21721 cgatgtatat ggtcgtaatt actatattta ttgatgatta ctatcttatt ttcctactac
    21781 aaggaatcta tattataggt gcagcactcg atatttcatg gttttatgct ggaactgaaa
    21841 agtttaaaat tcctagcctc agtaatattg ttgcgtctgg tattgtatta agtgtagttg
    21901 ttatttttgt caaagatcaa tcagatttat cattgtatgt atttactatt gctattgtga
    21961 cggtattaaa ccaattacct ttgtttatct atttaaaacg atacattagc tttgtttcgg
    22021 ttaattggat acacgtctgg caattgtttc gttcgtcatt agcatactta ttaccaaatg
    22081 gacagctcaa cttatatact agtatttctt gcgttgttct tggtttagta ggtacatacc
    22141 aacaagttgg tatcttttct aacgcattta atattttaac ggtcgcaatc ataatgatta
    22201 atacatttga tcttgtaatg attccgcgta ttaccaaaat gtctatccag caatcacata
    22261 gtttaactaa aacgttagct aataatatga atattcaatt gatattaaca atacctatgg
    22321 tctttggttt aattgcaatt atgccatcat tttatttatg gttctttggt gaggaattcg
    22381 catcaactgt cccattgatg accattttag cgatacttgt attaatcatt cctttaaata
    22441 tgttgataag caggcaatat ttattaatag tgaataaaat aagattatat aatgcgtcaa
    22501 ttactattgg tgcagtgata aacctagtat tatgtattat tttgatatat ttttatggaa
    22561 tttacggtgc tgctattgcg cgtttaatta cagagttttt cttgctcatt tggcgattta
    22621 ttgatattac taaaatcaat gtgaagttga atattgtaag tacgattcaa tgtgtcattg
    22681 ctgctgttat gatgtttatt gtgcttggtg tggtcaatca ttatttgccc cctacaatgt
    22741 acgctacgct gctattaatt gcgattggta tagtagttta tcttttatta atgatgacta
    22801 tgaaaaatca atacgtatgg caaatattga ggcatcttcg acataaaaca atttaagtac
    22861 cggtaatgct atactttaga aaattaagat taagaagaaa aggcaatttc ttattgaaaa
    22921 atggaagttg tcttttttaa ttctctttaa aagcgggaaa caaaagcagt taaatgcc
//
LOCUS       DAKTIP010000022        21779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAKTIP010000022 DAKTIP010000000
VERSION     DAKTIP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.022"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..981)
                     /locus_tag="O0305_000685"
     CDS             complement(<1..981)
                     /locus_tag="O0305_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0273841.1"
                     /translation="MAIKYSALNLVPIREGEDEQTAINDMVNLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNV"
     gene            1293..2435
                     /locus_tag="O0305_000686"
     CDS             1293..2435
                     /locus_tag="O0305_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0273842.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            2436..3554
                     /gene="mnmA"
                     /locus_tag="O0305_000687"
     CDS             2436..3554
                     /gene="mnmA"
                     /locus_tag="O0305_000687"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCY0273843.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            4016..4684
                     /locus_tag="O0305_000688"
     CDS             4016..4684
                     /locus_tag="O0305_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0273844.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            4686..7163
                     /locus_tag="O0305_000689"
     CDS             4686..7163
                     /locus_tag="O0305_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCY0273845.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSEEVMSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            7506..10136
                     /gene="alaS"
                     /locus_tag="O0305_000690"
     CDS             7506..10136
                     /gene="alaS"
                     /locus_tag="O0305_000690"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCY0273846.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNDEEVSQVEAGETVYFMLTETPFYAISGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            10199..10459
                     /locus_tag="O0305_000691"
     CDS             10199..10459
                     /locus_tag="O0305_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCY0273847.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            10463..10891
                     /gene="ruvX"
                     /locus_tag="O0305_000692"
     CDS             10463..10891
                     /gene="ruvX"
                     /locus_tag="O0305_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCY0273848.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            10906..11214
                     /locus_tag="O0305_000693"
     CDS             10906..11214
                     /locus_tag="O0305_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCY0273849.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            11499..12137
                     /locus_tag="O0305_000694"
     CDS             11499..12137
                     /locus_tag="O0305_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCY0273850.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            12140..13063
                     /locus_tag="O0305_000695"
     CDS             12140..13063
                     /locus_tag="O0305_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0273851.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            13075..14343
                     /locus_tag="O0305_000696"
     CDS             13075..14343
                     /locus_tag="O0305_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCY0273852.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            14343..14966
                     /gene="udk"
                     /locus_tag="O0305_000697"
     CDS             14343..14966
                     /gene="udk"
                     /locus_tag="O0305_000697"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCY0273853.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            14994..15470
                     /gene="greA"
                     /locus_tag="O0305_000698"
     CDS             14994..15470
                     /gene="greA"
                     /locus_tag="O0305_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCY0273854.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            15796..16530
                     /gene="pxpB"
                     /locus_tag="O0305_000699"
     CDS             15796..16530
                     /gene="pxpB"
                     /locus_tag="O0305_000699"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCY0273855.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            16520..17530
                     /locus_tag="O0305_000700"
     CDS             16520..17530
                     /locus_tag="O0305_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0273856.1"
                     /translation="MSIKILQPGLFSTVQDLGRIGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLADDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            17532..17981
                     /locus_tag="O0305_000701"
     CDS             17532..17981
                     /locus_tag="O0305_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCY0273857.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMIGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            17995..19356
                     /locus_tag="O0305_000702"
     CDS             17995..19356
                     /locus_tag="O0305_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0273858.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            19356..20108
                     /gene="pxpA"
                     /locus_tag="O0305_000703"
     CDS             19356..20108
                     /gene="pxpA"
                     /locus_tag="O0305_000703"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCY0273859.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            20121..21350
                     /locus_tag="O0305_000704"
     CDS             20121..21350
                     /locus_tag="O0305_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCY0273860.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
     gene            21639..>21779
                     /locus_tag="O0305_000705"
     CDS             21639..>21779
                     /locus_tag="O0305_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCY0273861.1"
                     /translation="MGYRTLKSIFHEHNESKMKEEYIKRFNSLASFNTNINIIPMENG
                     KKV"
BASE COUNT         7864 a         3009 c         4252 g         6654 t
ORIGIN      
        1 aacattcttg aaccgacgat aagagtctaa ttgcatatct ttatcataaa tataacttat
       61 tgccataatt tcgttgactt caccataagt ttccataaaa tcttgaattt tttgttgaac
      121 agtattttct gaacctatta atgatatttt ctgtctttcc atagccatcg cgtattctct
      181 cggcgttaat atattttgta gttcatgtgt tggcggttga acgggctgca ttctgccacg
      241 agtgatacta accattactt gtgccataga tgaagctaaa tattgtgctt cgtcatcatt
      301 ttcagcaacg attgtattta aacatacaat aacataaggt tcgtctaata catcagaagg
      361 ttcaaataac gttttgtaaa tttcgatagc ttctttcatt tgttgaggtg caaaatgtcc
      421 agcgaacaca tatggaagcc ctttgcgagc agctaaatgt gcagaatctg ttgaagaacc
      481 aagaatgtat aaaggcacat ttttacctac tgctggataa gcacgaacat atgcttgttg
      541 gtgagcaggg ccgaaatatt gttgtaataa tgaaacctct tctggaaatt tataaacacc
      601 atcatgttga tctcgtctta atgcacttgc ggtcatcata tctgttccag gtgcacgtcc
      661 taatcctaaa tcgacacgat ttggaaataa tgttgccatc gtgccaaatt gttccgcaac
      721 gattaatgga gcatgattag gtaacatgat gcctccagaa cctacacgta tgtgtttcgt
      781 atgttctaac gtatgttgaa ttaataaagc agttgctgaa cttactaggt tgggagcgtt
      841 atggtgttca gcaatccaat atctttcata tgataattcg tctaaatgtt gtgcgagatt
      901 aaccatatca ttaattgctg tttgttcatc ttcaccttct cgaataggga caaggtttaa
      961 tgctgaatat ttaatcgcca tttttatatc gtccttcctt ttttatgata taagaagtat
     1021 accaatcgaa taaccagata aatattaaat tgctcataac caaaataata gtacgttaca
     1081 ttttacaaat aaaaaaatat ctgcaaatac taaaatttat aaaaaagaca agaataaagc
     1141 gataaactta aaaaagccta tcgaaaagca tgtgtagaaa atggtaaaat ataattaaaa
     1201 acattataaa taaagttaaa cagaaaagta gaaaaatagt atgtaaggtg ctattattgg
     1261 aacattgata aaattgtgag gagtgtccaa acatggaaat ttatgcagat tatgctgcga
     1321 ccacaccagt aaaacctgaa gtagtagatg cgatgatgac gatttacaat tcgcattatg
     1381 gtaatccatc atcaatacat gctaagggaa gagatgcgcg taaatattta gatgagtcaa
     1441 gacgtcaaat tgcacaatta cttggtgcag atacacatga aattatattt acgagtggcg
     1501 caacagaatc gaataatacg gcaattaaag gcatagtaaa agcaaatgaa caattgggta
     1561 atcacattat cacatcgaag attgaacatc attctgtgtt acatgttttt gaacaattag
     1621 agcgagaagg ttttgatgta acatatctag atgttgatga tacaggtgct attgatttag
     1681 atcaacttga agaaacaatt acagataaga cgattttagt atctattatg tttgtaaata
     1741 atgaagttgg cacagtgcaa caaatttatg atattcaaga tattattgct gagacaaatg
     1801 catatttcca tgttgatgcg gttcaagcaa ttggacatct tgatgttaag tttgacgaat
     1861 ttgagatcga cgcaatgagt attactgctc ataagtttgg cggaccaaaa ggtgtcggtg
     1921 cattactagt taaagaccat gttacactag attatcctca acttggcggt gaacaagaat
     1981 tgaaacgccg tgcaggcact gaaaatcttg cgcaaattgt tgggatggct aaagcattac
     2041 aattagcaga aaagaatcga gacgataaca atattcattt aatgaattta aaagaacaat
     2101 ttttagttaa attacaagaa agagcgattc catttgaatt aaatggttca atgacggatg
     2161 ctacaggcca tattgtaaat ctatattttc cgtttgttga agttgaaacg atgttaacat
     2221 tgttggatat ggcacaaatc tatgtgtcgt ctggatctgc atgtacagca ggctctacac
     2281 aaccgtcgca tgttttagat gcaatgttcg aagatgagga gcgatcaaat cattcgattc
     2341 gatttagttt taacgaattg actactgaaa atgaaattaa tgcaattgta gctgaaattc
     2401 ataaaatata ttttaaattt aaggaggagt cataattgtc aaataaagat atacgtgttg
     2461 tcgttggtat gtcaggcggt gtagatagtt ctgtaacagc ccacgtctta aaagaacaag
     2521 gttatgatgt cattggcata tttatgaaaa actgggatga cactgacgaa aatggcgtat
     2581 gtactgcaac tgaagattac aacgatgtta ttgaagtgtg taatcaaatt ggcattccgt
     2641 attacgctgt taattttgaa aaagaatatt gggataaagt ctttacgtat ttcttagatg
     2701 aatacaaaaa aggtcgtact ccaaatccag acgttatgtg taataaagaa attaagttta
     2761 aagccttttt agatcatgcg atgaatttag gtgcagatta tgtagcaaca ggacattacg
     2821 cacgcataca tcgtcatgaa gatggtcatg ttgaaatgtt acgtggtgta gataataata
     2881 aagatcaaac atacttcttg aatcaattat ctcaacaaca actttcaaaa gtgatgttcc
     2941 caattggcga catcgaaaag agtgaagtgc gtcgaattgc tgaagaacaa ggacttgtta
     3001 ctgctaagaa aaaagattct acaggcattt gttttatcgg cgaaaaaaac tttaaaacat
     3061 ttttatcaca atatttacct gcacaaccgg gtgatatgat aacacttgat ggtaagaaaa
     3121 tgggtaaaca tagtggtttg atgtattaca caataggaca aagacatgga ttaggtatag
     3181 gtggagatgg cgatccttgg tttgttgtcg gtaaaaacct aaaagataat gttttatatg
     3241 tagaacaagg attccatcac gatgcattat acagtgatta cttaattgct tcagactatt
     3301 catttgtaaa tccagaagat aatgacttag atcaaggttt tgaatgtaca gctaaattta
     3361 gatatcgcca aaaagatacg aaagtttttg tgaaacgtga aaacgaccat gcactacgtg
     3421 ttacttttgc tgagccagta agagcaatca cacctggaca agcagttgtt ttttatcaag
     3481 gtgatgtgtg tcttggtggt gcaacaattg acgatgtgtt caaaaatgaa ggtcaattaa
     3541 attatgttgt ataaacaatg gcaacaataa attacttatt tgaagtttca acgttgaaaa
     3601 tgacgaaaga cagtttttga tgagaataat tcatgaggat agagtctggg acatcacaat
     3661 gtcctaggct ctacaatgtt atattggcgg gaccacaaca tagagaattt cgtaaagaaa
     3721 ttctacaggc aatgccagtt ggggataacg aatttaattt tgttaaaata tcatttctgt
     3781 cccactccct atgcatgaat ctaattatgt attcttattt ttaagtacat aatagtggtg
     3841 gctaatgtgg aagaaccatt acataataaa ccgttaatgg ttcttaagca tttctattcc
     3901 attcccgctt tttcatgaat gaagatgata ttagattata ttttattcgt tgttaagtga
     3961 ttcgagacat acaatttatc aagatgttat aattgatgag aaatgaggtt cgtaaatgat
     4021 agatcaacaa acaatttatc aatacataca aaatggaaaa atagaagaag cgttacaagc
     4081 attgttcgga aatatcgaag aaaatcctac aattattgaa aattatatta atgctggtat
     4141 cgtacttgct gatgcgaatg agattgaaaa ggcagagcgt tttttccaaa aagctttaac
     4201 aatagatccg aagaatggcg tcgtattttt taatctagca aatgtatatt ataatcagca
     4261 acgttatcaa gaagctatta aattatatca acaagcatta caaacagaga ttgaacaagt
     4321 tgattgtaat tatatgatcg gtatggcgtt taatcagtta gaatcattta agctggcatt
     4381 gccgtattta atgactgctg cggaactaga taaagacaaa gatgcagaag ttcaatttca
     4441 atatggtctt gtattatgtc aattagaaat gtttaatgaa gccataactc aacttaaaca
     4501 tgtattaacg attgataaaa atcatgttga tgcaagatac aatttgggct tagcgttatt
     4561 tatgaaaaat gaagatattg atgaagcaat aactcatttt aaagaagctg tgactatcga
     4621 ccctaaacac ttattaagtc agcatgcgct gaaaacattc actaaaatga aagaggagga
     4681 gtaacatgtc agaccctaca ctatttgatt attcaatgat caaaggtact gttgaagcga
     4741 tattgtttca aaacagtgat aatttttata cagtgcttaa agtagatact attgaaacaa
     4801 atgaagactt tgatacaatg ccaactgttg tagggtttct tccgaatatt gttgaaggcg
     4861 atgtctacac atttaaaggg caagttgttg atcatccacg ttatggtaaa caattaaaag
     4921 cagagacttt tgaaaaagaa atgccacaaa cgaaagaagc aattataagt tatttatcta
     4981 gtgatttgtt taaaggtgta ggtaaaaaga cagctcaaaa tattgttaat acattaggtg
     5041 acaatgcgat aaatgatata ttagatgacc attcagtgct agaaaaagtt tcgggattat
     5101 ctaaaaagaa acagaaacaa attgcagaac aaatttcagc aaatcaagaa tctgaaaaaa
     5161 taatgattcg tttacatgat ttagggtttg gtcctaaact ttcaatggca atttatcagt
     5221 tttatctcgg cgatacactg actattttag atcgaaatcc atatcaattg atttatgata
     5281 ttaaaggtat tggttttaat aaagcagatc aacttgctag aaatattggt atcgcttata
     5341 acgataatga acgattaaaa gctgcgttgc tctatacgtt agaagaagaa tgtattaaac
     5401 agggacatac atatttgcct atcaatgttg ttatagattt aacggttgat gtattaaatt
     5461 atcaagatga agaggtcatc gaacctgaaa aattagacga aatgttgcaa tacttaaatg
     5521 aggaaaaacg actaattatt gacaatgaac aagttgcaat tccaagtttg tattattctg
     5581 aaatcaaaag tgttcaaaac ttattcagaa taaagacgca tacaaataaa cttacagaaa
     5641 ttgaacagtc agatttacaa atgcatattg gtgaaataga agatgcgaat caagtgaact
     5701 atgcagcttc acaaaaagaa gctttgcaaa cggctatcaa ttctaaggtg atgctattaa
     5761 ccggtggacc gggaacagga aaaacgacag taattaaagg tattgttgaa ttatatgctg
     5821 agattcatgg cttatcatta gattatgatg attatgtcaa tgatgattat ccagttgtat
     5881 tagcagcgcc aacaggaaga gcgtctaaga gactacaaga gtcaactgga ttagaagcaa
     5941 tgacgattca tcgtttaata ggttggaatc aagacacaaa gccagaggat atattagaaa
     6001 atgaaataaa cgcacgactc ataatcattg atgagatgtc tatggtagat acttggttgt
     6061 tccaccaatt tttaagtgca gttcctttag atgcacaatt gatatttgta ggtgatgagg
     6121 accaattgcc ttctgtaggt cctggtcaag tatttaaaga tttaattgaa tcgaaagcta
     6181 taccacgagt aaatctgact gaagtatatc gtcaacaaga tggttcaagc attatagaat
     6241 tagcacatcg aatgaagttg ggtcagaaaa ttgatattac acagcgtttt catgatagaa
     6301 gttttattaa ctgtcaggca aaccaaatac caacagttgt tgagaaggta gttactagtg
     6361 ctgttaataa agggtatact atggctgata ttcaagtgct tgcaccaatg tataaaggaa
     6421 atgccggtat taaacgttta aatcaagttt tacaagatat tttaaaccct aaaaagaaag
     6481 acacacgaga aattgagttt ggtgatgtag tctttagaaa aggtgacaaa gtattacaac
     6541 ttgttaatag gccaaatgat aacatattca atggtgacat aggagttatt gttggtattt
     6601 tttgggcgaa agaaaatgcc cttaataaag atgtacttgt tgtggatttt gaaggtaatg
     6661 aaattacatt tacgaaacaa gatatgatgg aattaacaca tgcttattgt acatcaatcc
     6721 ataaatcaca aggttcagaa tttcctattg ttatcatgcc tattgttaaa cagtatttta
     6781 gaatgttaca aagaccgata ttatatactg gtttaactag agcgaaaacg agtttagttt
     6841 tacttggtga tccggaagca tttgatattg gattgaaaac aaatggtcaa gcgcgtttaa
     6901 cacaattatg tacattgttg aaaaattatt ttaattcaga agatgaagaa atgctggaaa
     6961 acacagcaac taatgataca ggagcttcac aaacgacaat agatgatcaa gtagaagcgc
     7021 caatgtcatc gaatgatagt gaagaagtta tgtctgattc aacaaaaaca gatacaaatg
     7081 tattaacgga agctacaatg tttaaaattg acccaatgat taatatggga gaaatcacgc
     7141 cgtatgactt tatagaacgt tgacaacaag cgtaaatgta actaaaatag aattaaattc
     7201 gtataaagat gagtagttaa tgatttgttt atcagagatg tactggttgg tgaaaagtac
     7261 aacaaacatt agtgaacgct actttatgca caactgtata gtcgaattaa agtgataaat
     7321 gtgttgaatt gcatttataa ctagggtggt accgcgataa cgttcgtccc ttttgaggat
     7381 gggcgttttt tattttttaa taataaaaaa tgatattgat ttatactagt tttgcaaatc
     7441 gactatctgt gtcatcaatt tttatggaat ggtaatttaa aaacagtaaa taggatgtgt
     7501 aaatgatgaa aaagttaaaa gcgagtgaaa ttagacaaaa atatctagat ttctttgttg
     7561 aaaaaggaca tatggttgaa ccttctgcac cattagtgcc aattgatgat gatacattat
     7621 tatggattaa ttcaggtgta gcaacattaa agaaatattt tgatggacgt gaaacaccta
     7681 aaaagccaag aattgtaaac tctcaaaaag ctattcgtac aaatgatatt gaaaatgttg
     7741 gtttcacagc gcgtcaccat acattctttg aaatgttagg taacttctca attggtgatt
     7801 attttaaaca agaagcgatt gaatttgctt gggaattttt aacgagtgat aaatggatgg
     7861 gtatggagcc agataaattg tacgttacga ttcatccgga agatatggaa gcatacaaca
     7921 tttggcataa agatattggg cttgaagaaa gtcgtattat tcgcattgaa ggtaacttct
     7981 gggatattgg tgaagggcct tcaggaccga acactgagat tttctatgat cgcggagaag
     8041 catatggaca agacgatccg gcagaagaaa tgtatccagg tggagaaaat gaacgctatc
     8101 ttgaagtatg gaacttagta tttagtgaat tcaatcataa taaagatcat agttacacac
     8161 cattacctaa taaaaatatt gatactggca tggggcttga gcgtatggcc tcagtttctc
     8221 aaaatgtacg tactaactat gaaacagatt tatttatgcc tataatgaat gaaatcgaaa
     8281 aagtatcagg taaacaatat ttagtaaaca acgaacaaga tgtggcattt aaagtaattg
     8341 ctgaccacat tcgtacgatt gcatttgcaa tttctgatgg tgcattacct gccaatgaag
     8401 gtagagggta tgtattacgt cgattgttac gtcgtgccgt tcgttttagt caaacgttag
     8461 gaatcaatga gccatttatg tacaaacttg ttgatattgt tgcagacatt atggaaccat
     8521 attatccaaa tgttaaggaa aaagcagatt tcattaagcg tgttataaag tctgaagaag
     8581 aacgattcca tgaaacatta gaagatggtt tagcgatttt aaatgaatta attaaaaaag
     8641 ctaaagcgac aacaaatgaa attaatggga aagatgcatt taaattgtat gatacgtatg
     8701 ggttcccaat tgaattaact gaagaaatag cagtgcaagc aggattgaaa gttgatatga
     8761 caacattcga gtcagaaatg caacaacaac gtgatcgtgc acgtcaagca cgtcaaaatt
     8821 ctcaatcaat gcaagttcaa agtgaagtat tgaaaaatat tacatctgca agtacttttg
     8881 ttggttatga tactgcgaca gctcaaacaa cactaacaca cttgatatac aatgatgaag
     8941 aagtttcaca agttgaagcg ggtgaaacag tatacttcat gttaacggaa acaccatttt
     9001 atgcaatcag tggtggacaa gttgcggata caggtattgt ttataatgac aattttgaaa
     9061 ttgctgttag tgaagtaacc aaagcaccaa atggtcaaaa cttgcataaa ggagtagtac
     9121 aatttggcca agtaaatgtt ggcgctacag tgtctgctga agtgaaccaa aatgatcgac
     9181 gtgacattca aaagaaccat agtgcaacac atttattaca tgcagcgttg aaatcagtac
     9241 tgggtgatca tgttaaccaa gctggttcac tagtagaagc agatcgttta cgttttgatt
     9301 tctctcattt tggtccaatg actaatgatg aaattgatca agttgaacgc ttagtaaatg
     9361 aagaaatttg gaaaggtatt gacgttaaca ttcaagaaat ggatattgct tcagctaaag
     9421 aaatgggcgc aatggcatta ttcggtgaaa aatatggtga tgttgtgcgt gtagtaaata
     9481 tggcaccatt ttcaattgaa ttatgtggtg gtattcatgt ccgcaatact tctgaaattg
     9541 gcttattcaa aatagtaagt gagtcaggta caggagctgg tgtgcgtcgt attgaagcat
     9601 taacaggtaa agcagctttc ttatatttag aagatattca agagaaattt aatacgatga
     9661 aatcacagct gaaagtgaaa tctgatgatc aagtagtcga taagttaaca caattacaag
     9721 atgaagaaaa agcattatta aaacaattag agcaacgtga caaagaaatc acatcactta
     9781 aaatgggtaa tattgaagat caagttgaag aaatcaatgg ctataaagta ttggttactg
     9841 aagtggatgt accaaatgcg aaagcaattc gctcgacaat ggacgatttt aaatctaaac
     9901 tacaagatac aattatcatt cttgcaagta atgttgatga taaagtatcg atggttgcaa
     9961 ctgtccctaa atctttaaca aataacgtta aagccggtga tcttatcaaa caaatggcac
    10021 caatcgttgg tggtaaaggt ggcggtcgtc cagatatggc tcaaggtggc ggtacacaac
    10081 ctgaaaatat ctcaaaatca ttaagcttta ttaaagatta cattaaaaat ctataattta
    10141 aagccataat attgtatgat gatagtgagt tatatttgta attaaaggag tgtcgcaaat
    10201 ggaaaacttt gataaaacaa tgaaattcga ctatgaagaa cttccaacgc aagatgtaag
    10261 agatgtttta aataatgttt atcgaacatt agatgaacga ggatataatg ccgtaaacca
    10321 aattgtaggt tatttattat caggtgaccc tgcgtatatt ccacgccaaa atgaagcaag
    10381 aaatcaaatt cgtcatattg atagagatgt tattatggaa gagcttgttt cttactattt
    10441 aaaagagcaa aataaataat ttatgttaca acataaaatt ttaggactcg atgtcggtag
    10501 tagaacggta ggaattgcaa ttagtgatat aatgggttgg acggcacaag gattagacac
    10561 actccgaatc aatgaagaaa ataatgaatt aggtattgac caattagtag acattattaa
    10621 aaaacataat gtgggtacag tcgtaatagg actacctaaa aacatgaata attcaatagg
    10681 atttcgaggc gaagcttcgt taacatataa agaaaaatta ttagaagctt atccttctat
    10741 tgagattgtg atgtgggatg aaagattaag cacaatggct gctgaacgat cattattaga
    10801 agcagatgtt tcaagacaaa aaagaaaaca agtgattgat aaaatggcag cggtatttat
    10861 tttacaaggc tatttagatt cactacatta aaaggagatt ttactatgac tgaacataat
    10921 catgattcac aactagaaat taataacgaa gaagaattat taactttatt cgatgaagag
    10981 ggaaatgaag ttttataccg aaaagtttta gaattttatc atcctgaatt caaaaaagag
    11041 tatgttatct tagctgaaga aggtgctcaa tcagatgaag acgatatgat tgagcttgta
    11101 ccaatgatca atgaaccaga tgagtcaggt gacggtggta agttagtacc aatcgaaact
    11161 gatgaagaat gggacatgat tgaagaagtt gtaaatactg aaatggaaga ataattaaaa
    11221 agattttact cttgctaacc ttaaagatgg ggaggcaaga gtatttttta agtcaagcaa
    11281 tgtataaata attaacgctt ataattttat tgaaaatatg gtaaaatatc taaagttaaa
    11341 ttttatatga atgattcgct tctataaata aaatagcttt gcgtctaaca cgaaatcagt
    11401 catttaattg gttggcaaaa ttataatcaa ttgtgaaggc tgatttattt tattgtcata
    11461 atcatattga cttattttac ataaaggaag tttaaaacat ggatgaccta aataaaaaat
    11521 atttaataga tttacatcaa catcaaaata gttcaatcga agttttgcgt gaatttgccg
    11581 aggtaaatga agtgccaatt gtagatcgtt taacattaga tttaattaag caattaattc
    11641 gtatgaataa tgttaaaaat attttagaaa ttggtacagc aatcggctat agttctatgc
    11701 aattcgcttc tatatctgat gacattcatg tcacaacgat agagcgtaat gaaacgatga
    11761 ttcaatatgc taaacaaaat ttagctactt atcattttga aaaccaggtt cgaattattg
    11821 aaggtaatgc tttagagcaa tttgaaaatg taaatgacaa agtttatgat atgatattta
    11881 ttgatgcagc aaaagcgcaa tcaaagaaat tttttgaaat atatacacca cttttaaagc
    11941 accaaggtct cgtaattaca gataatgttt tatatcacgg ttttgtatcg gatattggga
    12001 ttgttagatc gagaaatgta agacaaatgg ttaaaaaggt tcaagattat aatgagtggt
    12061 taataaagca accaggatat acaacgaatt ttttaaatat agacgatgga ttagcgattt
    12121 caattaaagg agaatgacta tgacagaatt acttgtcaca cccaagtcat taagccatat
    12181 ggaaacactt attgatttag gtgcagatgc ttttgtaatt ggcgagcaaa agtttggttt
    12241 aagacttcca ggggaattca atagacaaca aatgacagag gctgtcgcat tagctcacaa
    12301 aaacgataaa aaagtatatg cggcagtgaa tggattattt cataattatc atttagatgc
    12361 tgtagaagat tatattaatt ttttacatga aatacgagtt gacagaatta ttttcgggga
    12421 tccagcagta gtaatgtatg tgaaggcgca agaaaatcca ataccacttc attggaatgc
    12481 agagacgtta gtaactaatc attttcaatg taattactgg ggtaaaagag gcgcatcaag
    12541 agctgtctta gcacgtgagc tgaatttgga tgaaattatt aatataaaag aaaattcaaa
    12601 tgtggaaatt gaggtgcaag ttcaaggaat gacttgtatg tttcaatcaa agcgtatgct
    12661 actaggcaat tattatacgt tccaagatcg ccaaatgaaa attgaacgtc gcaatgatga
    12721 acaatcttta ttactttatg atgaagaaag acaaaataat tacccagttt acgaagatta
    12781 caatggtacg cacattatgt caccaaacga catttgttta attgaagaat tagcaccatt
    12841 ttttgaggcg ggtatagatt catttaaaat cgatggtatt ctacaaacgg aagaatatat
    12901 taatgtggta acggaacagt atcgtcaagc gatagatttg tacaatgaag atcctgaaat
    12961 ctatgaagat gagaaattta tgttgatgga tccaattgaa gaaattcaac ctgatcatcg
    13021 accatttgac gaaggtttct tatataaaca aacagtatat taaggaggtt aatcatgaag
    13081 acaatagaag agattaaatc aactcctaaa acagttatga agaaaccaga attattagca
    13141 cctgctggaa acttagaaaa gttaaaaata gcagtacatt atggcgctga tgccgtattt
    13201 ttaggtggtc aagaatatgg attacgttca aatgctgata atttcacgat ggaagaaata
    13261 gctgaaggtg ttgaatttgc gaaccgttac ggtgccaaaa tttatgttac gacaaatatt
    13321 attgcacatg atgagaatat tgaaggtcta gaatcatatt tgcgtaattt ggaaaagact
    13381 ggtgcgacag gtatcattgt tgcagatcct ttaattatcg aaacatgtaa agaagttgcg
    13441 ccaaaacttg aaattcattt atctactcaa caatcacttt ctaattacaa agctgtagaa
    13501 tattggaaag aagaaggatt ggatcgtgtt gtattagcac gtgagaccgg cgcgatggaa
    13561 atgcgtgaaa tgaaggaaaa agtagatatt gaaatcgaag catttattca tggtgctatg
    13621 tgtatcgcct attcaggtag atgtacatta agtaatcata tgactgcaag ggattccaac
    13681 agaggcggtt gctgtcaaag ttgccgttgg gattatgaat tattagaagt tgatgataat
    13741 ggtgaacttg atgtttttta taatcaaggt gaagttacac cgtttgcgat gagtcctaaa
    13801 gatttaaaat taatcgaatc aattcctcaa atgatggata ttggtgtgga ctcattaaaa
    13861 attgaaggac gtatgaagtc aattcattat attgcaacag ttgtctcagt atatcgtaaa
    13921 gtcattgatg cgtatgcggc agatcctgac aactttaaga ttaatccgga atggttaata
    13981 gagttagata aatgtgcaaa tagagacact gcaccagcat tctttgaggg aacacctggt
    14041 tatgaagaac agatgtttgg tcaacaacaa tctaaaaaat caccatttga tttttgtggt
    14101 ttggtattag actataatga agatacaaaa attgcgacta ttcaacaacg aaataacttt
    14161 aaaccaggtc aagaaataga attctttggt ccagaaattg aaacatttac acaagtagta
    14221 gaagcaattt atgatgaaga aggtaacagc ttagacgcgg cgcgccatcc attacaaatc
    14281 gttcaaatta aagtggatcg cccgatatat ccaaacaaca tgatgagaaa ggaaattggc
    14341 taatgaaagc tactacaatc attggcatag ctggtggatc tggctcagga aaaacaactg
    14401 taactaacga aattatgaaa aacttagaag gtcatagtgt cgctttactt gctcaagatt
    14461 actattataa agatcaaaag cacttgactt tcgacgagcg cctagaaacc aattatgacc
    14521 atccatttgc attcgataat gatttattaa ttgaaaatct taaagacttg aaaaatggta
    14581 aagcagtaga agtaccgaca tatgattatg ctagtcatac aagaagtgac attaccattg
    14641 attttaaacc taaagatgtt attatcgtag aaggcatttt cgctttagaa aataaggtat
    14701 tacgtgatat gatggatgtt aaaatatatg ttgatacaga tgcagacttg agaatattac
    14761 gccgtttaac acgagatact aaagagcgtg ggcgttcaat ggactctgtt atcaatcaat
    14821 atttaagtgt tgttagacct atgcatgacc aatttattga accgactaag aaatatgctg
    14881 atataattat tcctgaaggt gggagcaata aagttgcaat agatattatg acaacaaaaa
    14941 ttcagtcttt agttagcaag caatagtata attcagtaaa ggaagatgac aatatggaaa
    15001 atcaaaagca atatccaatg actcaagaag gttttgaaaa attagagcgt gaacttgaag
    15061 aattaaaaac agttaagcgt cctgaagttg tagagaaaat taaagttgca cgttcatttg
    15121 gtgacttatc agagaactct gagtatgatg cagcaaaaga tgaacaagga ttcatcgaac
    15181 aagatattca aagaattgag catatgttaa gaaatgcatt aatcattgaa gatactggag
    15241 ataacaacgt tgttaaaatt ggtaaaacag taacgtttgt agaattacca ggtgatgaag
    15301 aggaaagtta tcaaatcgtt ggttcagctg aatcagatgc atttaatggt aagatttcaa
    15361 atgaatcacc aatggctaaa gcgttaattg gtaaaggttt agatgatgaa gttcgtgttc
    15421 cactacctaa tggtggcgaa atgaacgtaa aaattgttaa tatccaataa atgatcaaat
    15481 agattattta attgcgcgat tgaagtgtgt taatgatact tcggtcgtgc ttttttatat
    15541 tcaaaatcaa aaaatcggac agatgaaaag taaaaatttt ccatcagacc gaatgatagc
    15601 agaacctaaa ttagtgtaag agttacaata ttaactactc cattttaaaa aagcaaaata
    15661 tttccgagaa aacaaattga ttatcatttg ttattgtatg cgatttgaat tacttattaa
    15721 acaacttgtg cttctcaatg atacaatgaa aaggtattca atactggttt attgcaatac
    15781 aaagggggat ttaaagtgga tgtaagattt attaacgagc agacaattat gatttatttt
    15841 gaaaataaaa tatccgaaga gacatatcgt aatgtcacag caatggtacg gtggatacga
    15901 gaaaaagaaa tattagaaat tcaagatatt gtcccttcgt atcgagcagt tctaatatat
    15961 tttgatgaac aagctataac atcttctaaa ttaattgaaa atttagaact taacaaattt
    16021 aatgaaaaaa atgttcatgc tgtgaatcaa accaatcgta ttattaaaat acctgttcaa
    16081 tatggtggta catatggacc agatattgaa gaagtagcaa agcataatcg aataactgtt
    16141 gagcaagtta ttgaaaaaca tacaagtaaa ccttatttaa tatatatgct aggatttatg
    16201 ccaggatttc catacttagg cggacttgat gaacaactac atacacctag acgtaatcag
    16261 ccaaggttaa aaattcatgc aggttctgta ggtattgcga ataatcaaac aggactatat
    16321 ccatcagatt ctcctggcgg atggcaaatt attggacgca caccgttaaa agtatttagc
    16381 agcgagcgtg aaccaatgtc aatgtacgaa gcaggagaat ggatacaatt ttatgcaata
    16441 gatgaacaga aatttataca aatcgaaaga gatatatcag atggcaactt taatgttgat
    16501 gactgggtgg tgattgaaaa tgtcaattaa gattttacaa ccaggtctct tttcaacggt
    16561 acaagatcta ggaagaatag gttatgaaca tattggattt tcaggcgcag gtgccatgga
    16621 tcaatttagt tttaaggttg cgcagtcatt aattaacaat gatggtccag cgattgaata
    16681 tactttgatt ggtcctacca ttcaatttaa tacgcaaaat acatttgtta taaccggtgg
    16741 tagtattaat gcttcgctaa ataataaaac tatatcaatg aattctgtca tattagctga
    16801 gaaaggtgac attttaaaaa taggtgctat aaccaaaggt gcacgcggtt atcttacttt
    16861 tggtcattct atcaacgtac cttctattgc tgaaagttat gcaacacata cccgaagcag
    16921 tataggagga ttcaaaggta gaaagttatt agctgatgat gtaataacag tgaaaataaa
    16981 taatgacttt aaagaaaaca ttggaaagac gattcattta caggacgatt tattgccaga
    17041 aaataatatt atacacattc ttcaaggacc tcaattcgag gcattttctg aagaggctag
    17101 agcgaaaatt gtaaatcatc catatttaat tactgaacaa tcagaccgta tgggctatcg
    17161 tttggaaggt gacagcgttg caccaattaa tcaagcagat atcatttctg aaccggttgc
    17221 tcttggtagt gttcaggtac caaatgatgg tcaacctatt attctgctta atgataaaca
    17281 aacgataggt ggttatacca aaattgcaac tgtatgcaaa tttgatctgc caaagttagc
    17341 acagatgaaa ccacaagata caattcaatt taaatggata tcattccaag aagctgtaga
    17401 taagaatcgg gaacaaatgt ctctgtttaa tgaaattctt aagagtcatc aaaagactcc
    17461 aatttttgat acatctagct tgcgacatac ttcgaaaaaa ttagcaacaa ttttaaaagg
    17521 ggatttgtaa aatgaatatt gaaaaaatcg aacaaataat caaattagtg aaggaaaatg
    17581 atgttaagaa atttaaatat aaaaattttg aagatgaaat agaaattgac ttcactgact
    17641 cgaatcattt ggctgcacac agtaatcaat caaatcaaag tatgaacaat aatgatttga
    17701 cagcttcaaa agcgaatgat aactccgatg tttcgacaaa tgattatcat gacattaaat
    17761 caccaatgat aggtacattc tttttacaag atagtaaaga attaactgaa ccaattgtga
    17821 atgtcggtga caaagttaac aagggagata ttataggata tgttgaagcg atgaaagtat
    17881 taaacgaggt aacaacagat gttgctggag aaattactga aatagtagct gatcatggca
    17941 caaatgttga atacgaccaa gttttggtac gtattaagta aagggggtaa gtaaatgctt
    18001 cgttgtttaa ttgcgaacag aggtgaaatt gctgtaagga ttataagagc ttgcagagaa
    18061 tatgggattg aaactgtagc agtttatgca aaaggggacg agcaaagctt gcatgtacat
    18121 ttagcagatc aagctatatg tattggggaa gctaatgctt tagatagtta tttaaatatt
    18181 gaccgcatca tatctgctgc acaaatcact ggtgctaatg caattcaccc aggatatggc
    18241 tttttatcag aatcaacaaa atttgctcaa accgttgagg aacaaggcat agcgtttata
    18301 gggcctacta aaaaaacgat ggaaatgatg ggggataaaa taacagcaag acaaacagtt
    18361 catcatgcag gtgttcctgt gattccagga tcaaatggtg ctgtgaatca tgtgtctgaa
    18421 attgagaatc ttgccaaaga catcggttat ccagttgtta tcaaagctgc cagtggcggt
    18481 ggtggtaaag gtatacgtat tgtaaagaaa gctgaagatt tagaaaaggc atttaaagaa
    18541 gctaaaagtg aaggaaaaaa atactttgat gatgatcgtg tttatgttga ggcttttata
    18601 cctgttgcaa aacatgttga agttcaagtt atgggagatg ggcaggataa ttatgtacat
    18661 ttaggggaac gtgattgttc tgtacaacgt aaaaatcaaa aattaatcga agaatctcct
    18721 tgtgcagcat taactgaaga aagacgacaa caaatatgta acgatgctgt taaagttgca
    18781 cgtgcagcaa attatagaag tgcaggtacg attgaatttt tagtaactga taccgcacat
    18841 tactttattg aaatgaatgc tcgaatacaa gttgaacata ctgttacaga gatgcgagca
    18901 gaacgtgatt tggttgcagc acaactgtat ttattggaac ataatcattt acctttttca
    18961 caaagcgata ttcaatttaa tggtcatgtg atagaagcga gaataaatgc cgaaaatcct
    19021 gaaaagaaat ttcaaccaac accaggtaaa gtgacagcgt tacatttacc tcaaggcttt
    19081 aatgtgcgag tcgattcttt actatatcac ggttatcaag tttcaccata ttatgattct
    19141 ttagtagcta aagtaattgt aaaatctcat gatagagctt cagcaattga taagttgaaa
    19201 gtcaccttag atgaaatggt gatagatgga ttttctacaa ctgccgattt tttatatgca
    19261 gttttaaatt atccactata tcgtgatgga gatgcaaaag atgttgatat taaattttta
    19321 gaaaaacatc aaattgttaa aggggtggaa ttataatgcg agttgatttg aattgtgatt
    19381 taggcgaagc atttggaaat tattcctttg gtggtgatca tcaaattatt ccgttaatta
    19441 caagtgcgaa tgttgcttgt ggttttcacg ctggtgatga aaatgtaatg aatgaaacgg
    19501 taaaacttgc caaagcacat aatgttgcag taggtgcaca tcctggttta cctgatttga
    19561 aaggctttgg cagacgaaat atagatatct ctaacgacga gatttataat ttgatgattt
    19621 atcaattagg tgcattacaa gggttttgtc gcattcatca agttaaaatt aatcatgtta
    19681 aaccgcatgg tgcattgtat cagatgggtg caaaagacag agaaatagca aacgttatag
    19741 cacaagctgt ttatgacttt gatccatcac tagtgttagt aggattagca aattcatatc
    19801 taatttcaga agcaaagaat gtcggattaa ttacagcttc tgaagtgttt gctgatagac
    19861 gatacgaaga tgatgggcag ctcgttagta gaaaagaaag tgatgctgtg attactgata
    19921 ctgacgaagc acttaagcag gttttaaaga tggtgaagga aaataaagtt atttcaaaaa
    19981 acaataagga agtaacgtta caagcagata caatttgtgt gcatggtgat ggagaacatg
    20041 cattattatt tgtttcgaaa attagagaaa ttttaatgaa agaaggcatt gatattcaat
    20101 ccttataggg ggctaaaaaa atggggagaa atttaaagct taaaaaagaa agtgactttg
    20161 aattcacaaa aaatcataaa aggttattat taggttctgt atttttgatg gcaacttctg
    20221 caattgggcc agcattttta acgcaaacag cagtatttac atcacaattt tttgcaagtt
    20281 tcgcatttgc catattactg tctatcatca ttgacattgg tgcacaaatt aatatatggc
    20341 gcatattagt tgtaactggt ttaagaggtc aagaaatatc aaataaagtt gttcctgggc
    20401 ttggtactgt tatctcaata ttgattgcat ttggtggtct agcttttaat attggtaata
    20461 ttgctggtgc cggtttaggt ttaaatgcaa tttttggatt agatgtaaaa tggggcgcag
    20521 ctattactgc aatctttgca atattaatct ttgtaagtaa aagtggccaa aaaattatgg
    20581 acgttgtttc aatgattctt ggtattgtga tgattttagt tgtggcatat gtgatgtttg
    20641 tttctaatcc accttatggt gatgcttttg tgcatacatt tgcgccagaa catccaatga
    20701 aattagtctt gcccatcatt acgttagttg gtggaactgt aggtggttat attacctttg
    20761 caggtgcaca tcgtatatta gactctggca ttaaaggtaa gcaatattta ccatttgtaa
    20821 atcaatcagc aattgctggt attttaacta caggtattat gagaacgtta ctattcctag
    20881 cggtattagg agttgttgta acaggtgtga cactaagttc tgaaaatcca ccagcgtcag
    20941 tttttgaaca cgcaattgga ccaattggaa agaatatttt tggtattgtg ttatttgctg
    21001 cagctatgtc atcagtaatt ggctcagcat acacaagcgc aacattttta aaaacacttc
    21061 ataaatcact taacgaaaga agtaatttaa ttgtgattgt gtttatcgtt atttcaacaa
    21121 tgattttctt atttattgga aaaccaatca gccttttaat tatagcaggc gcgataaatg
    21181 gatggatttt acctattacg ttaggtgcaa ttttgattgc aagtaaaaag aaatcaattg
    21241 ttggtgatta taaacaccca aattggatgt tcatctttgg tatcgttgca gtacttgtta
    21301 caatattgac aggtatattc tcattcaaag aagtacttca gttattttaa aagacatttc
    21361 gactaatagc atcactgtaa atgttgttag tattaggcaa gatatatttt gatttccggt
    21421 gattcattta taaagcataa ttagatatat tttaagggag agtggtgcat gacaaatgta
    21481 ctgctctttt tgttgtgatg aaaaatcttt ttctattgat tgagaaaata gttatttata
    21541 tagtttttaa agaaaaaaag tgataatatt tagctaatct aatgaaaatt gatttattgg
    21601 acaaaaaata cattttaaat gagtagagga ggctgcaaat gggttacaga actttaaaaa
    21661 gtatttttca tgaacacaat gaaagtaaaa tgaaggaaga gtatattaaa agatttaatt
    21721 ctttagcttc tttcaatact aatattaata tcatacctat ggaaaatgga aaaaaagtt
//
LOCUS       DAKTIP010000023        21473 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAKTIP010000023 DAKTIP010000000
VERSION     DAKTIP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.023"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            506..907
                     /locus_tag="O0305_000706"
     CDS             506..907
                     /locus_tag="O0305_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0273862.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(1027..1590)
                     /locus_tag="O0305_000707"
     CDS             complement(1027..1590)
                     /locus_tag="O0305_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0273863.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1587..2540)
                     /locus_tag="O0305_000708"
     CDS             complement(1587..2540)
                     /locus_tag="O0305_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCY0273864.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2650..3831
                     /locus_tag="O0305_000709"
     CDS             2650..3831
                     /locus_tag="O0305_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0273865.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            4119..6536
                     /locus_tag="O0305_000710"
     CDS             4119..6536
                     /locus_tag="O0305_000710"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCY0273866.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6558..6869
                     /locus_tag="O0305_000711"
     CDS             6558..6869
                     /locus_tag="O0305_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0273867.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            7195..13755
                     /gene="sasC"
                     /locus_tag="O0305_000712"
     CDS             7195..13755
                     /gene="sasC"
                     /locus_tag="O0305_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCY0273868.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQATNQALVNHNNGSIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVSNANNNDVVSNNTALNVPTKTNENGSGGHLT
                     LKEIQEDVRHSSNKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAVGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRIHGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVFVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSAAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNGLKRRAQT
                     ILDENRNNVPLNKRVSQAYIDSLTNQMQHTLIRSVDAENAVNKKVDQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDVTEDEIQDALNQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNADVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAETQHNTIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDTRNVVNDKAREAITNINATTGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKPAALAQI
                     NQHYNAKLAEINATPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAATNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVTPDHIVRAAARDAVKQQYEAKKREIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVETNGIATLKGVQPHIVIKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     FNDKKQAIEANIQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQTIH
                     DLDVHPIKKPDAEKTINDDLARVTALVQNYRKVSNRNKADALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKV
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKEIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(13872..15140)
                     /locus_tag="O0305_000713"
     CDS             complement(13872..15140)
                     /locus_tag="O0305_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0273869.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKHISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHASME"
     gene            15547..17208
                     /locus_tag="O0305_000714"
     CDS             15547..17208
                     /locus_tag="O0305_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCY0273870.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNIHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            17205..17900
                     /locus_tag="O0305_000715"
     CDS             17205..17900
                     /locus_tag="O0305_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0273871.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYIENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLTDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            17917..18339
                     /locus_tag="O0305_000716"
     CDS             17917..18339
                     /locus_tag="O0305_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0273872.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFKEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            19089..20498
                     /gene="sapep"
                     /locus_tag="O0305_000717"
     CDS             19089..20498
                     /gene="sapep"
                     /locus_tag="O0305_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCY0273873.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            20502..21350
                     /gene="dat"
                     /locus_tag="O0305_000718"
     CDS             20502..21350
                     /gene="dat"
                     /locus_tag="O0305_000718"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCY0273874.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
BASE COUNT         7802 a         3227 c         3936 g         6508 t
ORIGIN      
        1 atcacatttt atacacattt gatttgtaag aaatgttttg atttatacaa atcatatctt
       61 gaaaaataac caatttagcc tcattcggtt tgatttaatt tgttaaattt aaggctaact
      121 aattaattta gtttaatcaa ttttcatgag agttatatgt aataaaaatt caatgcgtat
      181 cttttttgaa gaaatatatg tagaattgtt gcaatttaat ggtaatattg atatattttc
      241 tttgtatata aattataaaa ttaatatgta atagagtgat ttgttttatg tactattatc
      301 ttatttctaa atattaactc tattgattat tggtttttat acttatttaa ttttattcaa
      361 ctttgacaat tgaatagaaa gcaagtttat ttacacttgt agttttatgc ataagttagc
      421 acatacaagt tttgggattg ttgggatgtt tgttaatact tgtatagtag ctaaatatgt
      481 gattattaat tgggagatgt ttagcatgaa aaaagtaaat aacgacactg tatttggaat
      541 tttgcaatta gaaacacttt tgggtgacat taactcaatt ttcagcgaga ttgaaagcga
      601 atacaaaatg tctagagaag aaattttaat tttactaact ttatggcaaa aaggttctat
      661 gacgcttaaa gaaatggaca gatttgttga agttaaaccg tataagcgta cgagaacgta
      721 taataattta gttgaattag aatggattta caaagagcgt cctgttgacg atgaaagaac
      781 agttattatt catttcaatg aaaagttaca acaagagaaa gtagagttgt tgaatttcat
      841 cagtgatgcg attgcaagta gagcaacagc aatgcaaaat agtttaaacg caattattgc
      901 tgtgtaagtt taatagcata aaaagaggtt ttcattaagt tgaaaacctc tttttgttgt
      961 tggcattaat ttttcaaatg ttgactactc aatcctaaat tataaatagt atagcgcagc
     1021 aaatgcttaa gaaatttttt ctatggcaca aatgaatgga gcatgattac gttggtttaa
     1081 aaattgatat tgcaaaactt gcgcatgctt ttgatccaaa gtactcaagt aatcaagcaa
     1141 tgcatgcttc tcaatttgtc cttcgctatg accatgatat ataacaagta caataatacc
     1201 ttcaattgac attaatgata gcaatgaatt aatagcttgg attgtcgtgt caggctttgt
     1261 cacgatagat ttatcacctt taggcaaata acctaggtta aagatggctg catcaatatg
     1321 acctttatgt gcatcattta tatgatgttc aatattttca tgtccatctt ttattaaaga
     1381 aacatgattg aaatccttaa ctttatcacg tgtattttcc aaagctaaat cttgaatgtc
     1441 gaaaccataa acatgtcctt ctggtacttg ttcggctaaa aataaagtgt cattgccgtt
     1501 accgcaagtt gcgtctacaa caatactttc tggtgttata tgttgtttaa taagtgtttt
     1561 tgaaaaaggg agtatacgtt ctaatttcat tgcttcacct tagacttgta acgcaagcct
     1621 tgataagaat ttctacgtgc taattcagca tcgatgccat ttaatacttc ccatttatta
     1681 acactccaca ttggacctac catgatatct attggaccat caccggtaat tcggtgaacg
     1741 atcatttcag ggggaatcac ttctaattgg tccacaacta ggtttgtgta ctcttcttga
     1801 gtcataaaag ttaataaacc tttatcgtat tgttttacca tcggtgtacc ttttaacaaa
     1861 tgaagtaaat gaattttaat accttgtaca tccatttgtg caacttcttt ggcagtagcc
     1921 atcatcatgt catagtcttc gccaggtaag ccattaatga tgtgtgtaca tacattgata
     1981 ttatgcttac gtaattttgc cacaccatca taataagttt tcatatcatg ggcacgattg
     2041 attaaatcag atgttgactg atggattgtt tgtagtccta attcaaccca taagtatgtt
     2101 cgttgattca aatctgctaa atattcgaca acatcgtctg gtagacagtc aggacgcgta
     2161 ccaatagata atcccacaac acccggttct ttaagtacag gttcgaattt ttcttttaat
     2221 acttcaaccg gtgcatgtgt atttgtaaat gcctgaaaat aagcaatata ttttccttcg
     2281 tgccatttct catgcatctt ttctttaatt tctttaaatt gtactgcgat tgaatctgca
     2341 cgattacctg caaagtctcc gctacctgca gcagaacaaa atgtacatcc accatgtgct
     2401 acagtgccat cgcggttagg acagtcaaac ccgccatcca atgcaacttt aaatattttt
     2461 tgtccaaatt tattttttaa atggtaattc catgtgtgat aacgtttgtt ttcaaaagcg
     2521 tattggaaat gattgcccat atgtcatttt cctttctata aaaaaagagt tctaagtaca
     2581 gattttaaca tattttaatg ttatagtgtt tattatagtt tgacaaaaaa gagagaggaa
     2641 ctatgaaata tgaatatacc taaatcagtc tggtggctag taattggcat ggcgttaaat
     2701 attactggtt ccagtttttt gtggccttta aatacaattt atatgaaaca agaacttgga
     2761 aaaagtttaa ctgttgctgg tttagtgcta atgataaatt catttggcat ggttattgga
     2821 aacttattag gtggttcact atttgataaa ttaggtggat acaagacgat tttaattgga
     2881 actttcactt gtctttgtag tacaacgcta cttaatttct ttcacgggtg gccttggtat
     2941 gctgtatggc ttgtaatgtt agggtttggt ggcggaatga ttattcctgc gatatacgct
     3001 atggctggag cagtgtggcc aaatggcgga agacaaacgt ttaatgcgat atacttagcg
     3061 caaaatattg gtgtggctgt cggtgctgca atgggcggct ttgtcgcaga atttagcttt
     3121 aactatatct ttttagccaa tcttattatg tatgttgtgt ttgcgcttgt cgcggtaacg
     3181 caatttaata ttgaaattaa tgcgaaagtt aaatatccaa ctcatttaga tattactggt
     3241 aaaaagaata aagcaagatt tatttcatta gtactaattt gtgcaatgtt tgcaatttgt
     3301 tgggttgcat atattcaatg ggagtctaca atcgcttcat ttacacaatc tattaatatt
     3361 tcaatggcac aatatagtgt tttatggaca attaacggaa taatgatttt agtagcgcaa
     3421 ccattaatta aaccgattct ctatctgtta aaaggaaact taaagaagca aatgtttgtc
     3481 ggcatcatca tttttatgtt gtcgttcttt gtcacgagtt ttgccgaaaa ctttacaata
     3541 tttgttgtcg gtatgattat tttaactttt ggagaaatgt ttgtatggcc agcagttcca
     3601 actatagcca atcagttagc gccagatggt aagcaaggac agtaccaagg ttttgtgaat
     3661 tcagctgcta cagtaggaaa agcatttggt ccatttcttg gtggtgtatt agttgatgcg
     3721 tttaatatgc gcatgatgtt tatcggtatg atgctactac ttgtatttgc attaatatta
     3781 ttaatggttt tcaaggagaa taatacgcaa cctaaaaaaa tagatgcata atgagtaaat
     3841 agaattaacg ttatagactt gaaataaatg tcgttataac ataatattaa tttgtataat
     3901 ttaatttcgt ttggagcttt tctacagaaa gctagtgatg ctgagagcta gtgttaagga
     3961 ctaaatgtaa atcgtattaa ttttaaattg aatgaatgac atctcttact attaaaatga
     4021 gtgcacaatt tttgtgaaat agggtggtaa cgcggcaaat gtcgtcccta tgtaaataga
     4081 atagttagag gtgtcttttt tattgaatag gaggaaatgt gttgaattat aaccacaatc
     4141 aaattgaaaa gaaatggcaa gactattggg acgaaaataa aacatttaaa acaaatgata
     4201 acttaggtca aaagaaattt tatgctttag acatgtttcc atatccatca ggtgctggtt
     4261 tacatgttgg acatcctgag ggctatacag caacagatat catttcaaga tataaaagaa
     4321 tgcaaggata taatgtatta catccgatgg ggtgggatgc attcggatta ccagcagagc
     4381 aatatgcttt agacactggc aacgacccac gtgaatttac aaagaaaaat atccaaactt
     4441 ttaaacgaca aattaaagaa ttagggttca gttatgattg ggatcgtgaa gttaatacaa
     4501 cagatccaga atactataaa tggacacagt ggattttcat acagttatat aacaaaggtt
     4561 tagcatacgt tgatgaagtt gcagttaact ggtgtccagc attaggcact gttttatcta
     4621 acgaagaagt gattgatggt gtctctgaac gtggtggaca tccagtttat cgtaagccga
     4681 tgaaacaatg ggtacttaaa atcacagaat atgcagatca attattagca gatttagatg
     4741 atttagattg gcctgagtct ttaaaagata tgcagcgcaa ttggattgga cgttctgaag
     4801 gggccaaagt ttcatttgat gtagataata cggaaggaaa agtagaagta tttacgacta
     4861 gaccagatac aatctatggt gcatcattct tagtcttaag tcctgaacat gcattagtta
     4921 attcaattac aacagatgaa tataaagaaa aagtaaaagc ttatcaaaca gaagcttcta
     4981 aaaagtcaga tttagaacgt acagatttag caaaagataa atcaggtgta tttactggtg
     5041 catatgcaac taatccttta tctggtgaaa aagtacaaat ttggattgct gattatgtat
     5101 tatcaacata tggtactgga gcaattatgg cagtaccagc gcatgatgac agagattatg
     5161 aatttgctaa aaagtttgat ttgccaatca ttgaagtcat cgaaggtgga aatgttgaag
     5221 aagcagcata cactggtgaa ggtaaacata ttaattctgg tgaacttgat ggtttagaaa
     5281 atgaagcggc aattactaaa gctattcaat tattagagca aaaaggtgct ggcgaaaaga
     5341 aagttaatta caaattaaga gattggttat tcagtcgtca gcgttattgg ggcgaaccaa
     5401 ttcctgtcat tcattgggaa gatggaacaa tgacaactgt tcctgaagaa gagctaccat
     5461 tgttgttacc tgaaacagat gaaatcaagc catcagggac tggtgagtct ccactagcta
     5521 atattgattc atttgtaaat gttgtagatg aaaaaacagg tatgaaagga cgtcgtgaaa
     5581 caaatacaat gccacaatgg gcaggtagtt gttggtatta tttacgttac atcgatccta
     5641 aaaatgaaaa tatgttagca gatcctgaaa aattaaaaca ttggttacct gttgatttat
     5701 atatcggtgg agtagaacat gcggttcttc acttattata tgcaagattt tggcataaag
     5761 tcctttatga tttggctatc gtacctacta aagaaccttt ccaaaaatta tttaaccaag
     5821 gtatgatttt aggagaaggt aatgagaaga tgagtaaatc taaaggaaat gtaatcaatc
     5881 ctgatgatat agtacagtct catggtgcag atactttgcg tctttacgaa atgtttatgg
     5941 gacctttaga tgctgcaatt gcatggagtg aaaaaggatt agatgggtct cgtcgattct
     6001 tagatcgcgt atggcgttta atggtaaatg aagatgggac attgagttca aaaattgtaa
     6061 ctacaaataa taaatcttta gataaagttt ataaccaaac tgttaaaaag gtaacagaag
     6121 actttgaaac attaggattt aatactgcta ttagtcaatt aatggtattt attaatgagt
     6181 gttataaagt tgatgaagtt tataaacctt acattgaagg cttcgttaaa atgttagcac
     6241 ctattgcacc acatatcggt gaagaattat ggtcaaaatt aggacatgaa gagtctatta
     6301 cgtaccaacc ttggccaact tatgacgaag cactacttgt agatgatgaa gtagaaatcg
     6361 ttgttcaagt gaatggtaaa ttgagagcta aaattaaaat tgctaaagat acatcaaaag
     6421 aagaaatgca agaaattgcc ttatctaatg acaatgttaa agcgagtatt gaaggtaaag
     6481 acatcatgaa agtcatcgct gttcctcaaa aattagtcaa tattgtagct aaataatgtt
     6541 ttaaggagga ctttgaaatg aagtcaatta ctacagatga attaaaaaat aaacttttag
     6601 aatctaaacc agttcaaatt gttgatgttc gtactgatga agaaacagca atgggatata
     6661 ttcctaatgc aaagttaatt ccaatggata ccattccgga taatttaaat tcatttaata
     6721 aaaatgaaat atattatatt gtatgtgctg gtggagttcg aagcgctaaa gttgtagaat
     6781 atttagaggc aaatggcatt gatgccgtaa atgtcgaagg cggcatgcac gcatggggcg
     6841 atgaaggttt ggaaataaaa agtatttaaa gtagtgacat aatttaaaat aatattacat
     6901 ttgtaatgac accaagtaac gtttcggttg cttggtgttt tttggtatga attactttct
     6961 gttacaaaac aatctaaagc gttcttgtta tgttttatta agattttaat tacaaaacgg
     7021 aaactaaatt gtaataaaat aaaactttat tttataaaat gatgatgata aaattgagtg
     7081 aacttaaaat attgtacaaa ataatatagc tataaatata atatgaggga gcgtatattt
     7141 ttagcataat tcttaacaac acagcagaga acagacaacc aggaggaaaa tgaaatgaat
     7201 ttgttaaaga aaaataaata tagtattagg aagtataaag taggcatatt ctctacttta
     7261 atcggaacag ttttattact ttcaaaccca aatggtgcac aagccttaac tacggataat
     7321 aatgtacaaa gcgatactaa tcaagcaaca cctgtaaatt cacaagataa agatgttgct
     7381 aataatagag gtttagcaaa tagtgcgcag aatacaccta atcaatctgc aacaaccaat
     7441 caagcaacga atcaagcatt ggttaatcat aataatggta gtatagtaaa tcaagctacg
     7501 ccaacatcag tgcaatcaag tacgccttca gcacaaaaca ataatcatac agatggcaat
     7561 acaacagcaa ctgagacagt gtcaaacgct aataataatg atgtagtgtc gaataatacc
     7621 gcattaaatg taccaactaa aacaaatgaa aatggttcag gaggacatct aactttaaag
     7681 gaaattcaag aagatgttcg tcattcttca aataaaccag agctagttgc aattgctgaa
     7741 ccagcatcta atagaccgaa aaagagaagt agacgtgcgg caccggcaga tcctaatgca
     7801 actccagcag atccagcggc tgcagcggta ggaaacggtg gtgcaccagt tgcaattaca
     7861 gcgccatata cgccaacaac tgatcctaat gccaataatg caggacaaaa tgcacctaac
     7921 gaagtgctgt catttgatga caatggtatt agaccaagta ccaaccgttc tgtgccaaca
     7981 gtaaacgttg ttaataactt gccgggcttc acactaatca atggtggcaa agtaggggtg
     8041 tttagtcatg caatggtaag aacgagcatg tttgattcag gagataataa gaactatcaa
     8101 gcacaaggaa atgtaattgc attaggtcgt atacatggaa ctgatacgaa tgaccatggc
     8161 gattttaatg gtatcgagaa agcattaaca gtaaatccga attctgaatt aatctttgaa
     8221 tttaatacaa tgactactaa aaacggtcaa ggcgcaacaa atgttattat caaaaatgct
     8281 gatactaatg atacgattgc tgaaaagact gttgaaggcg gtccaacttt gcgtttattt
     8341 aaagtacctg ataatgtgag aaatctcaaa attcaatttg tacctaaaaa tgacgcaata
     8401 acagatgcgc gtggcattta tcaactaaaa gatggttaca aatactatag ctttgttgac
     8461 tctatcggac ttcattctgg gtcacatgtt tttgttgaaa gacgaacaat ggatccaaca
     8521 gcaacaaata ataaagagtt tactgtaaca acatcattaa agaataatgg taattctggt
     8581 gcttctctag atacaaatga ctttgtatat caagttcaat tacctgaagg tgttgaatat
     8641 gtgaacaatt cattgactaa agattttcca agtaacaatt caggcgttga tgttaatgat
     8701 atgaatgtta catatgatgc agcaaatcgt gtgataacaa ttaaaagtac tggaggaggt
     8761 acagcaaact ctccggcacg acttatgcct gataaaatac tcgatttaag atataaatta
     8821 cgtgtaaata atgtgccgac accaagaaca gtaacattta acgagacatt aacgtataaa
     8881 acatatacac aagatttcat taattcagct gcagaaagtc atactgtaag tacaaatcca
     8941 tatactatcg atatcatcat gaataaagat gcattacaag ccgaagttga cagacgtatt
     9001 caacaagctg attatacatt tgcgtcatta gatatcttta atggtctgaa acgacgcgca
     9061 caaacgattt tagatgaaaa tcgtaacaat gtaccattaa ataaaagagt ttctcaagca
     9121 tatattgatt cattaactaa tcaaatgcaa catacgttaa ttcgaagtgt tgatgctgaa
     9181 aatgcagtta ataaaaaagt tgaccaaatg gaagatttag ttaatcaaaa tgatgaattg
     9241 acagatgaag aaaaacaagc agcaatacaa gttatcgagg aacataaaaa tgaaataatt
     9301 ggtaatattg gtgaccaaac gactgatgat ggcgttacta gaatcaaaga tcaaggtata
     9361 cagaccttaa gtggggatac tgcaacaccg gttgttaaac caaatgctaa aaaagcaata
     9421 cgtgataaag caacgaaaca aagggaaatt atcaatgcaa caccagatgt tactgaagac
     9481 gagattcaag atgcactaaa tcaattagct acggatgaaa cagatgctat tgataatgtt
     9541 acgaatgcta ctacaaatgc tgacgttgaa acagctaaaa ataatggcat caatactatt
     9601 ggagcagttg ttcctcaagt aactcataaa aaagctgcaa gagatgcaat taaccaagca
     9661 acagcaacga aaagacaaca aataaatagt aatagagaag caactcagga agagaaaaat
     9721 gcagcattga acgaattaac tcaagcaacc aaccatgctt tagaacaaat caatcaagca
     9781 acaacaaatg ctgatgttga taacgccaaa ggagatggtc taaatgccat taatccaatt
     9841 gctcctgtaa ctgttgttaa gcaagctgca agggatgccg tatcacatga tgcacaacaa
     9901 catatcgcag agatcaatgc taatcctgat gcgactcaag aagaaagaca agcagcaatt
     9961 gacaaagtga atgctgctgt aactgcagca aacacaaaca ttttaaacgc taataccaat
    10021 gctgatgttg aacaagtaaa gacaaatgcg attcaaggaa tacaagcaat tacaccagct
    10081 acaaaagtaa aaacagatgc aaaaaatgcc atcgataaaa gtgcggaaac gcaacataat
    10141 acgatattta ataataatga tgcgacgctc gaagaacaac aagcagcaca acaattactt
    10201 gatcaagctg tagccacagc gaagcaaaat attaatgcag cagatacgaa tcaagaagtt
    10261 gcacaagcaa aagatcaggg cacacaaaat atagtagtga ttcaaccggc aacacaagtt
    10321 aaaacggata ctcgcaatgt tgtaaatgat aaagcgcgag aggcgataac aaatatcaat
    10381 gctacaactg gcgcgactcg agaagagaaa caagaagcga taaatcgtgt caatacactt
    10441 aaaaatagag cattaactga tattggtgtg acgtctacta ctgcgatggt caatagtatt
    10501 agagacgatg cagtcaatca aatcggcgca gttcaaccgc atgtaacgaa gaaacaaact
    10561 gctacaggtg tattaaatga tttagcaact gctaaaaagc aagaaattaa tcaaaacaca
    10621 aatgcaacaa ctgaagaaaa gcaagtggct ttaaatcaag tggatcaaga gttagcaacg
    10681 gcaattaata atataaatca agctgataca aatgcggaag tagatcaagc gcaacaatta
    10741 ggtacaaaag caattaatgc gattcagcca aatattgtta aaaaacctgc agcattagca
    10801 caaatcaatc agcattataa tgctaaatta gctgaaatca atgctacacc agatgcaacg
    10861 aatgatgaga aaaatgctgc gatcaatact ttaaatcaag acagacaaca agctattgaa
    10921 agtattaaac aagctaacac aaatgcagaa gtagaccaag ctgcgacagt agcagagaat
    10981 aatatcgatg ctgttcaagt tgatgtagta aaaaaacaag cagcgcgaga taaaatcact
    11041 gctgaagtgg cgaagcgtat tgaagcggtt aaacaaacac ctaatgcaac tgacgaagaa
    11101 aagcaggctg ctgttaatca aatcaatcaa cttaaagatc aagcaattaa tcaaattaat
    11161 caaaaccaaa caaatgatca ggtagacaca actacaaatc aagcggtaaa tgctatagat
    11221 aatgttgaag ctgaagtagt aattaaacca aaggcaattg cagatattga aaaagctgtt
    11281 aaagaaaagc aacagcaaat tgataatagt cttgattcaa cagataatga gaaagaagtt
    11341 gcttcacaag cattagctaa agaaaaagaa aaagcacttg cagctattga ccaagctcaa
    11401 acgaatagtc aggtgaatca agcagcaaca aatggtgtat cagcgattaa aattattcaa
    11461 cctgaaacaa aagttaaacc agctgcacgt gaaaaaatca atcaaaaagc gaatgaatta
    11521 cgtgctaaga ttaatcagga taaagaagca acagcagaag aaagacaagt agcactagat
    11581 aaaatcaatg aatttgtaaa tcaagccatg acagatatta cgaataatag aacaaatcaa
    11641 caagttgatg atacaacaag tcaagcgctt gatagcattg ctttagtgac gcctgaccat
    11701 attgttagag cagctgctag agatgcagtt aagcaacaat atgaagctaa aaagcgcgaa
    11761 attgagcaag cggaacatgc gactgatgaa gaaaaacaag ttgctttaaa tcaattagcg
    11821 aataatgaaa aacgtgcatt acaaaacatc gatcaagcaa tagcgaataa tgatgtgaaa
    11881 cgtgttgaaa caaatggcat tgctacacta aaaggtgtac aacctcatat tgtaattaag
    11941 cctgaagcac aacaagcaat aaaagcaagt gcagaaaatc aagtagaatc aataaaagat
    12001 acaccacatg caacagttga tgaattagat gaagcgaatc aattaattag cgacacactc
    12061 aaacaagcgc aacaagaaat agaaaataca aatcaagatg ctgctgttac tgatgttaga
    12121 aatcaaacaa tcaaggcaat agagcaaata aaacctaaag taagacgtaa acgagctgcg
    12181 cttgatagca ttgaagaaaa taataaaaat caactcgatg caatccgaaa tacgttggat
    12241 actactcaag atgaaagaga tgttgctatt gatactttaa ataaaattgt aaatacaatt
    12301 aaaaatgaca ttgcacaaaa caaaacgaat gcagaagtgg atcgaactga gactgatggc
    12361 aacgacaaca tcaaagtgat tttacctaaa gttcaagtta aaccagcagc gcgtcaatct
    12421 gttggtgtaa aagccgaagc tcaaaatgca ctaatcgatc aaagcgattt atcaactgaa
    12481 gaagaaagac tagctgctaa acatttagta gaacaagcac ttaatcaggc tattgatcag
    12541 atcaatcatg cagataagac tgcccaagtt aatcaagata gtataaatgc tcaaaatatt
    12601 atttcaaaaa ttaaaccagc gacaacagtt aaagcaacag cattacaaca aattcaaaat
    12661 atcgctacaa ataaaattaa tttaattaaa gcaaataacg aagcgacaga tgaagaacaa
    12721 aatattgcaa tagcacaagt tgaaaaagag ttaattaaag ctaaacaaca aattgctagt
    12781 gcagtgacta atgcagatgt ggcatattta ttgcatgatg agaaaaacga aattcgtgaa
    12841 atcgaacctg ttattaacag aaaggcgtct gctcgagaac aattgacaac attattcaac
    12901 gataaaaaac aagcaattga agcgaatatt caagcaacgg tagaagaaag aaatagtata
    12961 ttagcacagt tacaaaatat ttatgacact gctattggac aaattgatca agatcgtagc
    13021 aatgcacaag ttgataaaac agcatcatta aatctacaaa caatacatga tttagatgta
    13081 catcctatta aaaagccaga tgctgaaaaa acgattaatg atgatcttgc acgcgtcact
    13141 gctttagtgc aaaattatcg aaaagtaagt aatcgtaata aggctgatgc attaaaagct
    13201 ataactgctt taaaattaca aatggatgaa gaattaaaaa cagcacgcac taatgctgat
    13261 gttgatgcag ttttaaaacg atttaatgtt gcattaagcg atatagaagc agtaattact
    13321 gaaaaagaaa atagcttact gcgaattgat aacattgctc aacaaacata tgcgaaattc
    13381 aaagcgatcg caacaccaga acaattagct aaagtaaaag tattaattga tcaatatgtt
    13441 gcagatggca atagaatgat tgatgaagat gcgacattaa atgacatcaa acaacacacg
    13501 caattcattg ttgatgaaat tttagcaatt aaattaccag ctgaagcgac gaaagtatca
    13561 ccaaaagaaa ttcagccagc tccaaaagtt tgtacgccta ttaaaaaaga agagacacat
    13621 gaatcgcgca aagttgaaaa agaacttcca aatacaggtt ctgaaggaat ggatttacca
    13681 ttgaaagaat ttgcactgat tacaggtgcg gctttgttag ctagaagacg tactaaaaac
    13741 gaaaaagaat cataattaac gaaatgacct taatataacg acacttttta gtgactcagt
    13801 tatattaagg tcatttttat ttgaatgaaa aagttgatct actaaagaga atcgaaccaa
    13861 attttatact attattccat tgatgcatgt gagtaatgtc cggcatataa tccagcgaca
    13921 tgtcctgtta cgagtgcact tgtaatatta taaccaccag tataaccatg tatatctaat
    13981 acttcaccac ataaaaataa tcccggaact aatttagaca tcattgtttt aggttgaatt
    14041 tctttaagtg acacaccacc acctgtgaca aatgccttat ctataggtaa tgtcccgttc
    14101 accttaaata caaacccttt aaacatattt actaagtcgt tcaattgttg atttgataag
    14161 tgatgtgatg tggtattttc atcgattcct gcttgttcca gcatgaacag taagtagcgc
    14221 tcttcaatta gaccatgcaa actgttttta atgattttat ctggtgtgtc cgataataat
    14281 gatgtgatgt gttgttttaa ttgttcatgg tttaattcag gaaatgcatc gattgccata
    14341 gaaatgtgct gtgtcttttg atttttttgt tctttataaa caaactgact acatcttaat
    14401 gcagctggac cactgatacc aaaatgagta aataacatat ccatttgatg actgatgtgt
    14461 tttttaccat ttttcttaag tactgacaat tcaacatctt ttaaacttaa accttttaga
    14521 cgattggatt tgatgaaagg ttcagctgat gtaattggaa cttcggtcgg gaataactca
    14581 gtaatggtat gacctaaatc ttgtgcgaac ttataaccat caccagttga accagtttga
    14641 gggacacttg taccacctgt agcaatcact agcgaatggc tttcataact attattttga
    14701 gtatgtacag tgaaagtttg gtctgtatta acttcgattc tactaacagc ttcttcttct
    14761 ttaatcgtta catgttggcg ttcgatagtt gtcactaatg tatcaaccac gtcttgtgct
    14821 ttgttggaaa ctggaaacat acgcccgtga tcttcttctt ttaatttaac acccctagac
    14881 tcaaaaaaat ctatgatgga ttcattatca aaaattgaaa agggactata taaaaatttc
    14941 ccatttccag gaatgttctt aataatttca gcatatggta atcgattagt tacgttacat
    15001 ctaccgccac cagatatttt gagtttacga cctagacctt tctttttttc aatgagtaac
    15061 acactgctac tttgttcgct tgcagctact gccgccatta agccgctagg tccgcctccg
    15121 ataataattg tttgatacat atacgcttcc tccaaatttg ttactggaca ttcaattgct
    15181 attttatagt acttttttaa actttgctat atctcttgtg ttatgagatg acaattatgt
    15241 tgaatatatt tataatgtga acttcataaa aaatgaagat gtatgtgtat gataagaatg
    15301 atttttctac tgttatgcga tgttcatctc aaatagtagt ataatgttta tatgaaattt
    15361 cgtaatattc taatcaaaat ctagtattca atgacaatgt gttgataatt ttgttatgat
    15421 aattcattga atatacgaca atgaaataaa gttttgtgat atattcataa taatgaaaaa
    15481 tgattttaca gagaaacagc ttttagttta caaaacttta ataaacatga gatagggaga
    15541 ttcgtaatga gtgaaagtaa agaaatggtg cgtggaacct ttttaattac cattagtata
    15601 ttaattacca aggtattggg cgtacttttt atcattccat tcaactattt aattggtgga
    15661 caagaaaata tggcgccgtt cacatacgct tatgcaccat ataatattgc aattgcagtt
    15721 gcgacagcag gtgtaccatt agcagcttcg aaatatgttg cgaaatataa tgctattggg
    15781 gcatataaag taagtcagaa attttataaa tcgagtttta ttgtaatgag tattaccggt
    15841 gttttaggtt tcttggtact gtatttctta gcaccttata tttctgaatt aacattagct
    15901 agaaacattc atgataaaaa cggttggtca gtcgatgata ttacttggat tataagaatc
    15961 attagtatgg ttgttatctt tattcctgta ttagcgacat ggagagggat attccaaggt
    16021 tataaatcta tggggccaac agcagtgtca gaagtaactg agcaaattgc acgcgtgatt
    16081 ttcatattaa tcggaagtta tttagtacta aatgtctttg atggctctat tttattagcc
    16141 aatggtattg ctacttttgc agcagcagtt ggtgcgatta ttggtatatt cacactctgg
    16201 tattactgga gaaagcgtaa gcataatatt gatcgtatgg tcgaatctga ttacacagat
    16261 atagatgtgt catatggcaa gatgtataaa gaaattattg cttatagtat accgttcgtt
    16321 attgtaagtt tgaattatcc attatttaac ttagtagatc aatttacaca taacggtgct
    16381 ttatctttag ttggtatacc ttcacagtta caagatatat tctttaatat gctaaatatg
    16441 tcaactaata aaattgtaat gattccgaca tcattaagtg ctggttttgc agtgagttta
    16501 attccttata ttacaaaaac atttgcagaa ggtcgcttac atgaaatgca tcatcaaatt
    16561 agaacatcta ttggtgtatt aatgtttata actgttccag ctagtattgg aattatggcc
    16621 ttggcacaac cattattcac agttttctat ggttacgatc caattgtttt aggtcatgac
    16681 cctaaccatg atggtagtcg cttattattc tattatgcgc ctgtagcaat actaatttca
    16741 ctattaagtg taacggcatc aatgttacaa ggtatagata aacagaaatt aacagtttat
    16801 gttattttag cttctgtagt tatcaagtta gcgttaaact atccattaat tatgttattc
    16861 catacacctg gtgcaatttt aagtactagt atcgcattat tatttgcaat tggatgtaat
    16921 ttctatattc ttaaaaaata tgcgaagttt aaattcagct atagttggat tcattttgca
    16981 aaaatattct tgtattcatt tattatgatg ctaggtgtag aattagtatt tttccttgca
    17041 aatctattct tagaacctac taaacttggt tacttaatca ttattatttt aggtgttaca
    17101 gtaggtattc taatttacgg tacaatcaca attaaaacgc gtcttgcgga cgaattctta
    17161 ggtgaaattc ctgaaaaatt aagacgtaga gttaggtttt tacgatgaga atagataaat
    17221 ttttagcaaa tatgggcgtc ggtacacgta atgaggtcaa acagttactt aaaaaaggcc
    17281 ttgtaaatgt caacgaacaa gtaattaaat caccaaaaac acatatagaa ccagaaaacg
    17341 ataagataac agttcgtgga gaattaatag aatacattga aaatgtctat attatgttaa
    17401 ataagccaaa aggttatata tcagcaactg aagatcatca ttcaaaaacc gttattgatt
    17461 taatacctga ataccaacat ctaaatattt ttccggttgg tcgtcttgat aaagatacag
    17521 aagggctttt attgataacg aatgatggtg attttaatca tgaattaatg agtccaaaca
    17581 aacatgtttc taaaaaatat gaagttattt cagcaaatcc tatcactgaa gacgacattc
    17641 aagcttttaa agagggtgta acactgactg atggcaaggt taagcctgca atattaactt
    17701 atattgataa tcaaacgtca cacgtaacaa tttatgaagg aaaatatcat caagttaaac
    17761 gaatgtttca tagcattcaa aatgaggtat tacatttaag acgtatcaaa attgctgatt
    17821 tagaattgga cagcaattta gattcaggag aatatcgttt attaactgaa aatgattttg
    17881 acaaattaaa ttataaataa aagggagacg attgatatgg caaaaggtaa tttatttaaa
    17941 gcgattttag gtataggtgg cgctgtagca gctgtacttg ttacacgtaa agatagtcgt
    18001 gacaagctga aagcagaata taataaatat aaacaagatc ctcaaagcta taaagataat
    18061 gctaaggata aagcgacgca attaggaaca attgctaatg aaacaattaa agaagtaaaa
    18121 acaaatccga aagaatatgc taatagatta aaaaataatc caaaagcatt tttcaaagaa
    18181 gaaaaatcaa aatttaccga atatgacaat aagactgacg aaagtattga aaaaggtaaa
    18241 tttgatgatg aaggtggcgc agcaccaaat aataatttac gtatcgtcac tgaagaagat
    18301 ttaaaaaaga ataaaaatgc attatctgat aaagaataag atgtgagtag gacagaaatg
    18361 ataagggagt gggacagaaa tcatatttta gcaaaattta tttcgtcgtc ccaccccggc
    18421 aaggttgact agaattgaaa aaagcttgtt acaagcgtat tttcattcag tcaactactg
    18481 ccaatataat attgtaaact ataggacatt tattagtgtt tcaggttctt tatcgttagt
    18541 catagctaat atgcatatag aaagaagaat acatgcgtga aactcattca taagaaatac
    18601 taatatctaa agaaaacgta tttctttatc ttttcgaaat tctctgtgtt ggggccctta
    18661 ccccaacttg cacattattg aaagctgact attggccagc ttctgtgttg gggcccttac
    18721 cccaacttgc acattattga aagctaacta ttggccagct tctatgttgg ggccccgcca
    18781 acttgcattg tctgtagaat ttcttttcga aattctctat gttggggccc cggggcgcat
    18841 tttcgttcgg ttaattactg tcaatattaa attgtgtgcc tagtacattg acttatatct
    18901 cgagctcttg aatatgtgta ttttaaatat ataaattaaa aaaggaacaa agcagctatt
    18961 tatgatttgt gtcatatcaa tagcatcgtt ttgttccttt ttattatttt ctgacatctt
    19021 attgtagttt ttatatcttt tcatgtaaaa tagaatttaa cttatagaaa aaaggaagtc
    19081 gatttcgaat gtggaaagaa aaagttcaac aatacgaaga tcaaatcatt aatgacttaa
    19141 aaggattatt agcaattgaa agtgtgagag atgatgcaaa agcatcagaa gacgcaccag
    19201 ttggtccagg tcctcgtaag gcattagact acatgtatga aattgcacat agagatggat
    19261 ttacaacaca tgatgtggat catattgcag gaagaattga ggcaggtaaa ggaaatgacg
    19321 tattaggtat cttatgtcat gttgacgttg ttcctgctgg tgatggatgg gatagtaatc
    19381 cgttcgagcc ggttgtaaca gaagatgcta tcatagctag aggtacactt gatgacaaag
    19441 gtccaacaat tgctgcttat tatgcaatta agatattaga agatatgaat gtggattgga
    19501 agaaacgtat tcatatgatt attggtacgg atgaagaatc tgattggaaa tgtacggatc
    19561 gctattttaa aacagaagaa atgccaacat taggttttgc accagatgca gaatttccat
    19621 gtattcatgg tgaaaaaggc attacaacat ttgatttagt tcaaaataaa cttactgaag
    19681 atcaagatga acctgattat gaattaataa cttttaaatc tggtgaacgt tacaacatgg
    19741 tacctgatca tgcagaagca agagtgcttg ttaaagaaaa tatgacagat gttattcaag
    19801 actttgagta ctttttagaa caaaatcatt tacaaggtga tagtactgtt gatagtggca
    19861 ttctagtttt aacagttgaa ggtaaagcgg ttcatggtat ggatccatct atcggtgtga
    19921 atgcgggtct ttacttacta aaattcttag catcattaaa tcttgataat aatgcacaag
    19981 cgtttgtagc atttagtaat cgctacttat ttaattcaga ttttggtgaa aagatgggaa
    20041 tgaaattcca tacagatgtc atgggtgacg tgacaactaa cattggtgtt attacatatg
    20101 ataatgaaaa cgcaggtctt ttcggtatca acttacgcta cccagaagga tttgaatttg
    20161 aaaaagctat ggatcgtttt gcaaatgaga ttcaacaata tggctttgaa gtgaaattag
    20221 gtaaagtcca accaccacat tatgttgata aaaatgatcc ttttgtacaa aagttagtta
    20281 ctgcatatag aaatcaaaca aatgatatga ctgaacctta tactataggt ggcggtactt
    20341 atgcgagaaa cttagacaag ggtgtagcat ttggcgcaat gtttagtgat tctgaagatt
    20401 taatgcatca gaaaaatgaa tatatcacta aaaaacagtt atttaacgca actagtattt
    20461 acttagaagc aatttattca ttatgcgtgg aggaataata tatggaaaaa atttttttaa
    20521 atggtgagtt tgtaagtcca agtgaagcaa aggtttcata caacgacaga ggatacgtat
    20581 ttggcgatgg tatttatgaa tacattcgag tatataatgg taagttattt acagtaacag
    20641 aacattatga aagattttta cgtagtgcca atgagattgg tttagattta aattattctg
    20701 tagaagaatt aattgaacta tctcgtaaat tagttgatat gaatcaaatt gaaactgggg
    20761 caatttatat tcaagcaacg cgtggtgtag ctgaaaggaa tcatagcttc ccgacacctg
    20821 aagtagaacc agcaattgtt gcttatacaa agagttatga tcgtccttat gatcatttag
    20881 aaaatggtgt gaatggtgtt accgttgaag atatccgatg gttacgttgc gacattaaaa
    20941 gcttgaactt attaggaaat gtattagcaa aagaatatgc tgtgaaatat aatgcagttg
    21001 aagcaattca acatcgaggt gaaactgtaa ctgaaggatc ttcaagtaat gcttatgcaa
    21061 ttaaagacgg tgtgatttat acacatccga ttaacaacta tattcttaat ggtattacac
    21121 gaattgtaat taaaaaaatt gccgaagact ataacatccc atttaaagaa gaaacgttta
    21181 ctgtagattt cttgaaaaac gcagatgaag ttattgtttc aagtacttca gctgaggtta
    21241 cacctgttat taaattagat ggtgaaccag ttaatgatgg taaagttggc ccaattacac
    21301 gtcaactaca agaaggattt gaaaagtata tagagtcaca cagtatttaa aattctttca
    21361 tcatattttt agattaaatt acttaaattt atcaactttc gtaagtgaat tatgttataa
    21421 tttaaaacga agcttaaaag tgttgtttag aaaacacata ttagtaaaaa gtg
//
LOCUS       DAKTIP010000024        21043 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAKTIP010000024 DAKTIP010000000
VERSION     DAKTIP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 21043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21043
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.024"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            71..1102
                     /locus_tag="O0305_000719"
     CDS             71..1102
                     /locus_tag="O0305_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCY0273875.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            1445..2563
                     /locus_tag="O0305_000720"
     CDS             1445..2563
                     /locus_tag="O0305_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0273876.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            2538..3461
                     /locus_tag="O0305_000721"
     CDS             2538..3461
                     /locus_tag="O0305_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCY0273877.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVIAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            3472..4692
                     /locus_tag="O0305_000722"
     CDS             3472..4692
                     /locus_tag="O0305_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0273878.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     TDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(4797..6329)
                     /locus_tag="O0305_000723"
     CDS             complement(4797..6329)
                     /locus_tag="O0305_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCY0273879.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVVNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(6369..7250)
                     /locus_tag="O0305_000724"
     CDS             complement(6369..7250)
                     /locus_tag="O0305_000724"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCY0273880.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            7408..8268
                     /locus_tag="O0305_000725"
     CDS             7408..8268
                     /locus_tag="O0305_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCY0273881.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(8546..9346)
                     /locus_tag="O0305_000726"
     CDS             complement(8546..9346)
                     /locus_tag="O0305_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0273882.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            9486..10154
                     /locus_tag="O0305_000727"
     CDS             9486..10154
                     /locus_tag="O0305_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCY0273883.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     IMED"
     gene            complement(10281..11594)
                     /locus_tag="O0305_000728"
     CDS             complement(10281..11594)
                     /locus_tag="O0305_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0273884.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
     gene            12012..14084
                     /gene="lip2"
                     /locus_tag="O0305_000729"
     CDS             12012..14084
                     /gene="lip2"
                     /locus_tag="O0305_000729"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCY0273885.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
     gene            complement(14326..15153)
                     /locus_tag="O0305_000730"
     CDS             complement(14326..15153)
                     /locus_tag="O0305_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0273886.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMM"
     gene            complement(15226..16425)
                     /locus_tag="O0305_000731"
     CDS             complement(15226..16425)
                     /locus_tag="O0305_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0273887.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNTSTKEGYITKEDL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLINWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGDHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            16521..16613
                     /locus_tag="O0305_000732"
     CDS             16521..16613
                     /locus_tag="O0305_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273888.1"
                     /translation="MESIFKIKLMNGICRSENMNMKKKNKGEKI"
     gene            16727..17728
                     /locus_tag="O0305_000733"
     CDS             16727..17728
                     /locus_tag="O0305_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCY0273889.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEDFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEKVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            17762..18094
                     /locus_tag="O0305_000734"
     CDS             17762..18094
                     /locus_tag="O0305_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCY0273890.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            18095..18895
                     /locus_tag="O0305_000735"
     CDS             18095..18895
                     /locus_tag="O0305_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCY0273891.1"
                     /translation="METLKSNKARLEYLINDMHRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LSAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEAFNRDAE"
     gene            18885..19829
                     /locus_tag="O0305_000736"
     CDS             18885..19829
                     /locus_tag="O0305_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCY0273892.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLHRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITAALRNDSTTKNNNIGETEDVLNRTD"
     gene            19807..20829
                     /locus_tag="O0305_000737"
     CDS             19807..20829
                     /locus_tag="O0305_000737"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0273893.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELTDADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFSNVTVEAL
                     VDMILS"
BASE COUNT         7174 a         3284 c         4153 g         6432 t
ORIGIN      
        1 tggcctctaa gtattcaata tttaaatatg gggattgaat ataaaattat cgtaatgggg
       61 gtcaatggtt atggatttat tgataggtac tttattttta tttttggtct tagtgatttt
      121 tacattattt acatataaag cgcctaatgg tatgcgtgcc atgggagcat tagctaatgc
      181 agcaatcgca acatttttag tggaagcatt taataaatat gttggtggcg aagtattcgg
      241 tattaaattt ttagaagagc taggagacgc tgcgggaggt ctaggtggtg tcgctgccgc
      301 tggattaaca gcattagcta tcggtgtgtc accagtatat gcattagtta tagcagccgc
      361 gtgcggtggt atggatttat taccaggttt ctttgcgggt tatatgattg gatatgtgat
      421 gaaatataca gagaaatatg tgccggatgg tgtcgactta attggatcga ttgtcatctt
      481 agcgccatta gctcgtctta ttgcagtatt attaacgcca gtagtgaata gtacattgat
      541 tcgaattggt gatattatcc aaagtagtac gaatacgaat ccaattatca tgggtatcat
      601 tttaggtggt attattacgg ttgtcggcac agcgccattg agttcaatgg cattgacagc
      661 attattaggt ttaacgggtg tacctatggc tattggtgcc atggcagcat ttagttcggc
      721 atttatgaat gggacgctat tccatcgctt aaaattaggt gatcgtaagt ctacgattgc
      781 agtaagtatt gaacctttat cacaagcaga tattgtatca gccaatccaa ttccaatcta
      841 tattacaaat ttctttggtg gtgcgattgc tggtttaatt attgctatgt caggtttaat
      901 taacgatgcg acaggtacag ctacaccgat tgcaggattt ttagttatgt ttggatttaa
      961 tcatccgacg acaattgtga tttatggtgt agtaatggcg attgtaggtg cgcttgcagg
     1021 ttatcttggt tcaattgtat ttaaaaaata tccaattgtt actaagcaag acatgattaa
     1081 tcgaggtgca gtagacgcat agcatcatca tattgaatag taaaaacaaa taaaacatag
     1141 taacgtgatt cagtcgatgt aacagtcgat aatgagtcac gtttttttat agaaaaatac
     1201 aagacataaa aatgtcataa tttattgtcg acaaatatca tactgtataa acatttatca
     1261 ttttctcaag taccttttac acgatggaat gaacttactt tttacgaaat tatgcgtatt
     1321 ttataaacaa atatcattga tataacggta aatgtaagcg tttacaacag aaataacagc
     1381 atgctacgat atttttgtaa attcactgat tcaagtattt taagtcaata tgaggaggga
     1441 tgttatgagc gattctgaga aagaaatttt aaaaagaatt aaagataatc cgtttatttc
     1501 acaacgtgaa cttgctgagg caattggatt atctagaccc agcgtagcaa acattatttc
     1561 aggattaata caaaaggaat atgttatggg aaaggcatat gttttaaatg aagattatcc
     1621 tattgtttgt attggcgcag cgaatgtaga tcgtaagttt tatgtgcata aaaatttagt
     1681 tgcagaaaca tcaaatcctg taacgtcaac acgctctatt ggtggcgtag caagaaatat
     1741 tgctgagaac ttaggtaggc ttggcgaaac ggtcgctttt ttatctgcta gtggacaaga
     1801 tagtgaatgg gaaatgatta aacgattgtc cacaccattt atgaatttgg atcatgttca
     1861 acaatttgaa aatgcgagta caggttcata tacagcttta attagtaaag aaggcgacat
     1921 gacatatggc ttagcagata tggaagtgtt tgactacatt acgcctgaat ttttaattaa
     1981 gcgttcacac ttattgaaaa aggctaagtg cattattgta gatttgaatt taggcaaaga
     2041 ggcattaaac ttcttatgtg cctataccac gaaacatcaa atcaaattag ttatcaccac
     2101 ggtttcttcc ccaaaaatga aaaatatgcc tgattcatta catgctattg attggattat
     2161 cacgaataaa gatgaaacag aaacatactt aaatttaaaa atagaatcta ctgatgattt
     2221 aaaaatagct gctaaacgct ggaatgattt aggtgttaaa aatgttattg tgacaaatgg
     2281 cgtgaaagaa ctcatttatc gaagtggtga ggaagaaatc attaagtcag ttatgccatc
     2341 aaatagtgtg aaagatgtta caggtgcagg cgattcattc tgtgctgcag tagtgtatag
     2401 ctggttaaat gggatgtcta ctgaagatat attaattgct ggtatggtta acgcaaagaa
     2461 aacgatagaa acgaaatata cagttaggca aaacctagat caacagcaac tttatcacga
     2521 tatggaggat tataaaaatg gcaaatttac aaaagtatat tgagtattct cgagaagttc
     2581 agcaagcacg ggagaacaat caaccgattg tagcattaga atcaacaatt atttcgcatg
     2641 gtatgccgta cccacaaaat gttgaaatgg caacaacagt agagcaaatt atcaggaata
     2701 atggtgccat tccagcaacc atagccatta tagatggcaa aattaaaatt ggtttagaaa
     2761 gcgaagattt agaaatactg gcaactagta aagacgttgc taaagtatct agaagggatt
     2821 tagcagaagt tattgcgatg aagtgtgttg gtgctactac tgtagcgacg acgatgatat
     2881 gtgctgcaat ggctggtatt caattttttg ttacaggagg tattgggggc gtccataaag
     2941 gtgcagaaca tacgatggac atttcagcag acttagaaga actgtctaaa acaaatgtca
     3001 ctgttatctg tgcaggtgcc aaatcaattt tagacttacc taagacgatg gagtatttag
     3061 aaacaaaagg cgttccagtt attggatatc aaacgaatga attgccagca ttcttcactc
     3121 gcgaaagcgg tgttaagtta acaagttcgg ttgaaacgcc agaacgactt gctgacattc
     3181 atttaacaaa acagcagtta aatcttgaag gtggcattgt tgttgctaat ccaattccat
     3241 atgagcatgc cttatcaaaa gcatatattg aggcaatcat aaatgaagct gttgttgaag
     3301 cggaaaatca aggtattaaa ggtaaggacg ccacaccgtt cttgttaggg aaaattgtag
     3361 aaaaaacgaa tggtaaaagt ttagcagcaa atataaaact tgttgaaaac aatgcggcgt
     3421 tgggtgctaa aattgctgtc gctgttaata aattattgta ggtgatgata catgaatatt
     3481 ttattcgcta tcacagggat agcatttgca ctatttgttg cgtttttatt cagttttgat
     3541 cgtaaaaaaa tagacttcaa aaagacgtta ataatgatat ttattcaagt gttgatcgtg
     3601 ttatttatga tgaacacaac gattggtttg acaattttaa ctgcactagg ttcatttttt
     3661 gaagggctaa taaatattag taaagcaggc ataaattttg tttttggaga tatacaaaat
     3721 aaaaatggct ttacgttctt tttaaacgta ttactgccat tagtttttat ttctgtatta
     3781 ataggcatct ttaattatat taaggtatta ccatttatta tcaaatatgt aggtatcgct
     3841 attaataaaa taactagaat ggggcgctta gaaagttatt ttgctatttc aacagcaatg
     3901 tttgggcaac cagaagtata tttaacaata aaagatatta ttccaagatt atctagagcg
     3961 aaattatata caattgcgac gtctggtatg agtgctgtta gtatggcaat gctaggttca
     4021 tatatgcaga tgattgaacc caagttcgta gttacagcag taatgttaaa tatttttagt
     4081 gcgcttatca tcgccagtgt aatcaatccc tataaatctg atgatactga tgttgaaatt
     4141 gataacttaa cgaaatccac agaaactaaa acattgaatg gaaaaacagg aaaacctaag
     4201 aaagttgcct ttttccaaat gattggtgat agtgcgatgg atgggtttaa aatcgctgtt
     4261 gtagtagccg taatgttgtt agcatttatt tcattaatgg aagcaattaa tatcatgttt
     4321 ggtagtgttg gtttgaactt taaacagctt attggctatg tgtttgcacc aatcgcattc
     4381 ttaatgggga ttccatggag cgaagctgtt ccagctggct ctttaatggc gactaaatta
     4441 attacaaatg agtttgtagc aatgcttgat tttaaaaatg tcctgggtga tgtatcagct
     4501 cgaacacaag gtatcatttc agtttactta gtaagcttcg ctaattttgg tacggttggt
     4561 atcatcgtag gttcaattaa aggcattagt gataaacaag gagaaaaagt tgcatccttt
     4621 gcaatgaggt tgctacttgg ttcaactcta gcttcaatca tttcaggatc aatcattggc
     4681 ttagtattgt aaatgaatcg aagtacctaa attaaattca tggcaaagct aaaccccgtc
     4741 accaagttgg cgcaacagcg catcataact tagtgacggg gttttatcat aacaatctac
     4801 tttttcgtag ccgtttttga aatgtatgtt gatggtttat ctttttcaaa aattgttaat
     4861 cccgttatat cttttttatg ttttgaaggg acaatgaagc taagtatata agcaaagaca
     4921 aaagcaactg taaatgaaat ggtagataca tagaaaggtg agttaccttt gccaacacca
     4981 ttatagacat aagcaaagat gatacccaat attaatccac aaataacacc gaatgtattc
     5041 gtacgtttag tgaaaatacc aactgcaaat acaccagcca atggaacgcc gaataatcca
     5101 gtcacaaaca agaataaatc ccataagtca tttgaattag aagcaattaa gtatagtgac
     5161 attccaaaac cgaaaatacc tgcaatgata ataatgaaac gtgcaaagtt aacttcgtgt
     5221 cgctcgctac cttttccgaa gaagcgttgc ttaatgtcga ttgaaataca agcagatata
     5281 gaatttaaac tagatgaaat ggtagactgt gcagcggcga aaatggctgc aataagtaat
     5341 cctgctacaa atggtggcat ctcagtcaaa atgaaatatg gcactacaga tgatgtattg
     5401 aagccttttg gtaaaacagc ttcatgtgta taaaatgaat acagcattgt acccatacca
     5461 taaaataagg gtgctgaaat taaagctagg ataccatttg tccataacga tttatttgtt
     5521 tcttttaaac tatcagaagc ttgataacgc tgcacgacgt cttgactcgc tgtgtattga
     5581 tacaagttgt tgaaaatatt tcctaggaaa ataattggaa tggcagctgc cgcagtattt
     5641 agtttccaat tgtctgcact aattaatttt ttgtgctcaa tcgcatctgc aaagacagtg
     5701 ccgaaaccgc ctttaatgtt cacaacacct agaataataa taactaaagc gccgcctaat
     5761 aaaatgacgc cttgaatgaa atcactccaa accacacctt cgaaaccacc taaaaatgta
     5821 tataaaatac atagtaaacc aacgagtgat gcaacgatat aagggttcat gtctgataca
     5881 gatgtgattg ctaatgttgg taagtagata acaattgcaa cacgccctaa atggtaaacg
     5941 acaaataata atgagccaat gacacgtatg ctagggccaa atctagcttc taaatattca
     6001 tatgcagatg ttacctttaa ctttttaaag aaagggacat agaaataaat aagtaatgga
     6061 ataattgcga cgatagcaat gttaccagcg atatatgacc aatctgttaa aaatgctttc
     6121 tctggtgtcg acataaatgt aatcgcactt aacgtagtag cataaattga aaagccaact
     6181 acccaagatg gcaagcgacc acttgcggta aagaaactat tggtactttg gctcgcgcgc
     6241 ttggtaaaat aaacgccaat gaacaacata gctagtagat aaatgataac ggcaacccag
     6301 tttagtgtgc caaatccaac ttctttcatg ggcaacatcc cctttacaat gtattgattc
     6361 tttgatgtct ataaatcgta ttttgcaatg agttgatcta atgtttgtcg atgtgcttcg
     6421 ttaaaaggtt tgaaaggtct tttcggtaat cctgcatcaa tgccacgatg acgtaatatt
     6481 tctttcaatg ttggataaat ccccattgat aacactgttt cgataatgtc gtttgaatca
     6541 tgttgcagtt ggtaagcttc ttgaatttga ccttgtcgtg ctaagtcgaa gatttttctt
     6601 gcacggcgac cattaacgtt atatgtagaa ccaattgcac catctacgcc agaaatcgta
     6661 gcttgaacta acatttcatc aaagccagat aagattaatt tgtctgggaa tgcttttcta
     6721 atacgttcga gtaggaagaa gtttggcgct gtatatttaa caccaacaat tttttcatga
     6781 ttaaatagct cgctgaattg ttcaatagaa atattcacac ctgttaaatc tggtattgca
     6841 taaataatca tattgttctg agttgcttcg ataatatcga aatagtaatc tctaatttct
     6901 tcaaaagtaa atggatagta gaatggtgtt acggcagaaa gtgcatcata accgagttct
     6961 gtggcatatt ttccaagttc aatggcttca tttaaatcta acgaacctac ttgagcaatc
     7021 aatttcactt tatccccaac tgcctctttg gcaaccttga aaacttgctt cttctgctct
     7081 gtatttaata aaaagttttc gcctgagcta ccatttacat aaagaccgtc taattcttca
     7141 gtttcaatgg cattttgagc aatttgttta agtccttgtt catttacttg accattttca
     7201 tcaaaaggaa cgagtaacgc tgcatataaa ccttttaaat ctttgttcat tatgaagtcc
     7261 ctccaaaaat catttgataa tatagtttac agctataatt gtaaacgcta tcataaaatg
     7321 taacaatatc tttttgaaaa ttgtagtcat atttatgtat aattaatgaa aatgtttttc
     7381 aaaatcaata gaaatggagt gagtaaggtg tattacatcg caatcgatat tggaggcact
     7441 caaattaaat cggcagttat tgataagcaa ttgaatatgt ttgactatca acaaatatca
     7501 acgccggaca acaaaagtga gcttattact gacaaagtat atgagattgt aacaggatat
     7561 atgaagcaat atcagttgat ccaacctgtc ataggtattt catcagcagg cgttgttgat
     7621 gaacaaaaag gcgaaattgt atacgcaggg ccaaccattc cgaattataa aggtactaat
     7681 tttaagcgat tattaaaatc actgtctcct tatgtcaaag taaaaaatga tgtaaacgct
     7741 gcattactag gcgaattgaa attacatcaa tatcaagcag aacggatctt ttgtatgacg
     7801 cttggtacag gcattggggg tgcgtacaag aataatcaag gtcatattga taatggtgag
     7861 cttcataagg caaatgaagt tgggtattta ttgtatcgtc caactgaaaa tacaacgttt
     7921 gagcaacgtg ctgcaacgag tgcattgaaa aagcgcatga ttgccggagg atttacgaga
     7981 agcacacatg tgccagtatt gtttgaagca gctgaagaag gtgatgatat tgcaaaacaa
     8041 atattgaatg agtgggcaga agatgtagca gaagggattg cccaaataca ggtcatgtat
     8101 gatccagggc ttatattaat tgggggcggt atatctgaac aaggagataa tctcattaaa
     8161 tatatcgagc cgaaagttgc acactattta ccaaaagact atgtttatgc accaatacaa
     8221 acgactaaga gtaaaaatga tgcagcatta tatggctgtt tgcaatgata gttgaaagaa
     8281 ggagtcattc taaaatagaa tttgaaaccg ttacgagaga tgagagctgt tgttagttcc
     8341 acacatcaca ctctatctag gaccaatcta aactatatca accaacagtg tgccacgggc
     8401 aaattaaatt gaagaagctg agatattaaa attttagaaa atgtaaaaaa atatttggta
     8461 ttgaaattaa aaaagcacct agcaactcgt tgggacaatc acgatgattg tctacagttg
     8521 caggtggatt tgaatatact actagttatt tgttgtctag gataatagat ttagtatgtt
     8581 gataagtttg actcagattc gtattttcta ataaatgata actcacgata tcgattaaaa
     8641 agagtgtcgc aatttgtgtg ttgataaatt gatggtcggt attacgcgat tgatccgttg
     8701 ttaaaagtac taaatctgca caatctgtaa gtttactacc ttcaaaattt gtgatggcaa
     8761 cgacatatgc accatgagat ttggcgactt ccgctgcaga aattaattcc gaagtattac
     8821 cactatttga catagcaata aacatatccg aatgagatag tagggatgcc gatattttca
     8881 ttaaatgtga atcggtagta acattacctt ttagccccat acgaatcata cgataataaa
     8941 attcagtcgc tgataaacca gagctaccta gtccagcaaa gagtatatgt cgacttgatt
     9001 gaagtttgtc gataaaggtt tggataatgt cgttatcaat aaattcacca gtttgttgaa
     9061 tgatttgttg atgatattta tgaattcttt gaataattgg gctattttca ataactgtct
     9121 ctgtcatttc ttgttgaata ttaaatttta aatcttggaa attctcataa tccagcttat
     9181 gactaaagcg tgtcatcgtt gctggtgatg taccaatcgc atgggctaag gagttaatcg
     9241 ttgaaaaggc atcgctataa ccattttgtc ttatataatt gacgatgcgt ttatcagttt
     9301 ttgtaaataa atgttgataa cgttgaacac gattctcaaa tttcattgtg tcaccccttc
     9361 atcttaatga ttactattat atatgaaaaa tattttcaag atagtaaaaa gcattgataa
     9421 aaattatctt aatgatatat tgtaaatgac tttacgtgaa aaaacgactt atggagtgag
     9481 gaataatgtt accacatgga ttaatagtat cttgtcaggc actaccagat gaaccattgc
     9541 attcatcttt tattatgtcg aaaatggcat tagctgcgta tgaaggtggt gctgttggta
     9601 ttcgcgcaaa tactaaggaa gacattttag caattaaaga aacggtagat ttaccagtta
     9661 ttggcattgt gaaacgtgac tatgatcact cagatgtttt cattactgca acgtcaaaag
     9721 aagttgatga actgatagaa agccaatgtg aagtcattgc attggatgca acgttacagc
     9781 aacgtccgaa agaaacgtta gacgaattag tatcatatat tagaacacat gcaccgaacg
     9841 ttgaaatcat ggctgatatc gcgaccgttg aagaagctaa aaatgccgca cgacttggct
     9901 ttgattatat tggcacgacg ttacatggct atactagtta tacgcaagga caattacttt
     9961 atcaaaatga cttccaattt ttaaaagatg tactacaaag tgttgatgca aaagttattg
    10021 cggaaggtaa tgtcattaca ccggatatgt ataaacgtgt gatggactta ggcgttcatt
    10081 gttcagtcgt tggtggtgcg ataacacgac caaaagaaat tacgaaacgt tttgttcaaa
    10141 ttatggaaga ttaaatgata acgataaaaa aacgagatga ccatcattaa ttaaaggcac
    10201 ctaattatct taggtggctg aatgaatgta atgggttcat ctcgttttgt ttgtttatga
    10261 tagtgatttt attttcaact ttatccaaaa ataagtaaag cgacggggat ggtgattaat
    10321 agcgacaacg ccacgcgtaa aaaccaaatg atgatgagtt tccagacagg tattttaatt
    10381 tcagttgcta gtatacatgg cactaatgct gagaaaaaga taatggctga tacgcttact
    10441 acaccgacga caaatttagt actcattgca gctttagtta ctaacaaaga tggtagaaac
    10501 atctctacaa tagaaatcgc tgacgctttt gctagtaaag cctgatcagc aattgggaaa
    10561 atataaataa atggatagaa gatatagcca agccaatcaa tgaatggtgt atagttcgct
    10621 acaatcagtc ctaaaaaacc aatcgataat atagaaggta aaataccaac agtcatttct
    10681 aaaccgtctt tcaaattgtc ccaaacgttc ttcacgagag atggtgttaa tgcattttgt
    10741 ttcatcgcct ctgcatatgc agttttcagt ctgcttcctt caatagcaac ttcttgttct
    10801 ccttcttgtc cgttataata ttctgttgat tcattgctga ttggcggtag ccatgcagta
    10861 attgcagtca cgacaaatgt gatgactaaa gttatccaaa agtataaatt ccaatgcggc
    10921 attaatccta aagttttagc aacgataatc ataaaagttg ctgaaactgt tgaaaagcca
    10981 gtcgcaataa tcgtggcttc tcgtttgttg tacatccctt gcttatagac acgattagta
    11041 atcaataatc ctaaggaata actgccgaca aacgaagcca ctgcatcgac agcggatttt
    11101 cctggtgttt taaaaatagg tctcataata ggctccatat aaacaccgac aaattctaat
    11161 aagccatagc ccactaataa agaaagcgca attgcaccta ctggaattaa gatacttaat
    11221 ggcatcatta atttttcaaa caaaaacgga ccatagttag ctttaaatag tattgatgga
    11281 ccgattttaa atacatacat tataccgatc attgcacctg caactttaaa taatgtaatg
    11341 accaagtttg tgattgaagt cataaaagta cgtctcacta ttggtaacgc tgtaccaatt
    11401 aaaatcataa tcagtgcaac atagggcata agtggaccta tgattgagcg aatggctaga
    11461 tgaacatgat cgacgaaaat agtgttgtta ccattaatcg taaaaggaat aaagaaacat
    11521 agtatgccca ctaaactata gacaaaaaaa cgccatgcac ttggttgttg tgcattagaa
    11581 tgatattgat tcattaaagc aacccctttg tttaaatgaa tacacaaaac tgtatgatgc
    11641 atcttcccct taatgagatg aatcattatt ttaatttaga aaaatctgaa aacttactat
    11701 aattgtatag tttgaattat tttcatacca atacaaatta actaattata tatagattga
    11761 aactatatta cttaataaaa tatttatctt aaatgttgtt gtgttgattc aacaccacaa
    11821 ctaaaagtgt ttataaatta tttggaaata cacatatttg taaatgatta gtatcgattt
    11881 aatatcgtat tattaaattt ttattaattt tgtagtctta atcaaaaaat aatatatgtc
    11941 atgttatatt gaaggtgcag ttgtttttca ttctcaagag ggggtcaaaa aaatactttt
    12001 gaggtgatta tatgttaaga ggacaagaag aaagaaagta tagtattaga aagtattcaa
    12061 taggcgtggt gtcagtgtta gcggctacaa tgtttgttgt gtcatcacat gaagcacaag
    12121 cctcggaaaa aacatcaact aatgcagcgg cacaaaaaga aacactaaat caaccgggag
    12181 aacaagggaa tgcgataacg tcacatcaaa tgcagtcagg aaagcaatta gacgatatgc
    12241 ataaagagaa tggtaaaagt ggaacagtga cagaaggtaa agatacgctt caatcatcga
    12301 agcatcaatc aacacaaaat agtaaaacaa tcagaatgca aaatgataat caagtaaagc
    12361 aagattctga acgacaaggt tctaaacagt cacaccaaaa taatgcgact aataatactg
    12421 aacgtcaaaa tgatcaggtt caaaataccc atcatgctga acgtaatgga tcacaatcga
    12481 caacgtcaca atcgaatgat gttgataaat cacaaccatc cattccggca caaaaggtaa
    12541 tacccaatca tgataaagca gcaccaactt caactacacc cccgtctaat gataaaactg
    12601 cacctaaatc aacaaaagca caagatgcaa ccacggacaa acatccaaat caacaagata
    12661 cacatcaacc tgcgcatcaa atcatagatg caaagcaaga tgatactgtt cgccaaagtg
    12721 aacagaaacc acaagttggc gatttaagta aacatatcga tggtcaaaat tccccagaga
    12781 aaccgacaga taaaaatact gataataaac aactaatcaa agatgcgctt caagcgccta
    12841 aaacacgttc gactacaaat gcagcagcag atgctaaaaa ggttcgacca cttaaagcga
    12901 atcaagtaca accacttaac aaatatccag ttgtttttgt acatggattt ttaggattag
    12961 taggcgataa tgcacctgct ttatatccaa attattgggg tggaaataaa tttaaagtta
    13021 tcgaagaatt gagaaagcaa ggctataatg tacatcaagc aagtgtaagt gcatttggta
    13081 gtaactatga tcgcgctgta gaactttatt attacattaa aggtggtcgc gtagattatg
    13141 gcgcagcaca tgcagctaaa tacggacatg agcgctatgg taagacttat aaaggaatca
    13201 tgcctaattg ggaacctggt aaaaaggtac atcttgtagg gcatagtatg ggtggtcaaa
    13261 caattcgttt aatggaagag tttttaagaa atggtaacaa agaagaaatt gcctatcata
    13321 aagcgcatgg tggagaaata tcaccattat tcactggtgg tcataacaat atggttgcat
    13381 caatcacaac attagcaaca ccacataatg gttcacaagc agctgataag tttggaaata
    13441 cagaagctgt tagaaaaatc atgttcgctt taaatcgatt tatgggtaac aagtattcga
    13501 atatcgattt aggattaacg caatggggct ttaaacaatt accaaatgag agttacattg
    13561 actatataaa acgcgttagt aaaagcaaaa tttggacatc agacgacaat gctgcctatg
    13621 atttaacgtt agatggctct gcaaaattga acaacatgac aagtatgaat cctaatatta
    13681 cgtatacgac ttatacaggt gtatcatctc atactggtcc attaggttat gaaaatcctg
    13741 atttaggtac atttttctta atggctacaa cgagtagaat tattggtcat gatgcaagag
    13801 aagaatggcg taaaaatgat ggtgtcgtac cagtgatttc gtcattacat ccgtccaatc
    13861 aaccatttgt taatgttacg aatgatgaac ctgccacacg cagaggtatc tggcaagtta
    13921 aaccaatcat acaaggatgg gatcatgtcg attttatcgg tgtggacttc ctggatttca
    13981 aacgtaaagg tgcagaactt gccaacttct atacaggtat tataaatgac ttgttgcgtg
    14041 ttgaagcgac tgaaagtaaa ggaacacaat tgaaagcaag ttaaattcat cttctgaatt
    14101 taatatgcta tgtaaatcgt gctgttatca cggcacatca gatataagta gcatcacagt
    14161 gttgaattta aaaatagtaa agtgaaataa agcgcctgtc tcattagcga aaactaaagg
    14221 gacaggcgta tctgtttatg agcttaataa attgtatgaa taatatggtt gatcgaataa
    14281 ctgtttatca tgatgataaa ttgagttttt taaaataatg atatattaca tcattgttat
    14341 agcgtttaag aaatcaacaa ctttacgata aatagtgatt gcttcgtcat taggtctacg
    14401 atcaaaatca tgctcgtttt tattcacgcg ttcaaatgtt gaatgtggaa catgattcat
    14461 gatatgttcg ctttcctcaa cgggaacatc ataatcgcca ttacaatgcg caatgaaaac
    14521 aggtggaagt gttttaagtt catctggtgc aatattatat tttgaattag tataatcagc
    14581 aatgttaatc atatttatcc atttacctgt gccacgtgca taaacgtaga ttaaaaaacg
    14641 ttgtgcgatt tgatcttgaa caaccggtgt tggtgaagtg agttgtgcaa tcattgtttc
    14701 gtttacgctt tgagctattt ttgcgtaata actattagtt gttttaaaag gttcagtgtt
    14761 gatgcgacta taaccataaa aatcaataac accatcaata tctctgtctc gtgcaattaa
    14821 tagacttaaa tatgcacctg atgatctgcc aaaggtaaaa atagggcaat tagaatattg
    14881 tgattgaatc gcatcgaatg atgcgtagac atcctcaata atgcaatcga gacttacttc
    14941 tggtaataaa cgataactta gttgaattaa atcgtaatgt tccgtaagga tatcgatata
    15001 ctgtggggat aaatcgttag ctttaccgaa cattaatcca ccaccgtgga tgtagacaat
    15061 agcacctttt gttggttgat tttttgcttt aataattgtg taaggtaatg caaatgcatc
    15121 tttagtaatt actttatctt taatttcagt cacgatttaa taggctcctt atttttgata
    15181 ttgatgtcat tataacactg tcttaaattt ccatgaaaaa tagtcttaag acgatgagtc
    15241 atgataattc tgttccaatt gacgtaaagc gtcacgggta tgcttcttta gaccttcccc
    15301 ataatccatc attttaacaa tatctttaaa agcagcatgt ggaatggcta aatcttctaa
    15361 atctgccata gaaaattcaa gattgatatc atgtggtcgc tgttcagcaa gtttatgcac
    15421 aaagtcaggt tctgtgacaa aaggcgaaga catgccgacc atatctgcat gttgtaaagc
    15481 atctaaagca gactctggag aattaatccc gccacttgca attaaaggga tacgacctgc
    15541 taaatgttca tagacaattt ggttaactgg tcgaccgaaa tgatcacctg gtgtacgaga
    15601 cgtattttga taaatatgtc gaccccagct agcgattgct aagtattgga tgtttgaaac
    15661 gtccatgacc caattgatta attggttgaa ctcgtcaatg gtatatccta aatcactgcc
    15721 tctggtttct tctggcgttg ctcgaaatcc taaaataaaa ttgtcaggtg cttctttatc
    15781 aatcacttct tgtaccgcac gcataacttc taaacataat cttgcacgat tttttaatga
    15841 gtcggcaccg taatggtctg tacgtttatt cgaaaaagtt gagaaaaatg tttgaatcag
    15901 caaacgttgt gcaatcgaaa tttccacacc atcaaaacct gctttaatcg cgcgtaatgt
    15961 agcatcgcga tactgctgaa tgatgctatt gattttctca tgagacatgg cgataacatc
    16021 gtgttcaatc ggtgaatgca atgtcatagg gcttggtcca tacacctttc caaaatttaa
    16081 aatggcttga tttgaaaaac gaccagcatg cgctagctgg ataatagcga ggctaccatg
    16141 ttgtttcatc gtagatgcca tgttagttaa tccagggata caagcatcat gatcaatatt
    16201 aaagccatat tcaaacaatt gaccataagg ttcaatgtaa gcagcgccgg tgacttgcat
    16261 tccagctgaa ttagagcgac gtgcagcata agccaagtct tcttttgtaa tatagccttc
    16321 ttttgttgat gtgtttacgg tcattggtga taatacaaag cgattcgaaa ttttgatgcc
    16381 attaggtaag tggattgatt gtaaaagtgg tttgtatcgg tacatactat gattcctttt
    16441 ctattcaata ttgttttcaa agtaccatgg aaagaatgaa taatcaatga tgaacagtct
    16501 tgatagaata gaattggtac atggaaagta tttttaaaat taaactaatg aatggcattt
    16561 gtaggtctga aaatatgaat atgaaaaaga aaaataaagg cgaaaagata taaaagttaa
    16621 ttgaaaaacg ttatcatata cgtgggtata tgaagaggga atggtattaa gaacgctaaa
    16681 atgttatgtc ggtttgacat gacaggataa gtttggagat gacggattgg ttaaattaag
    16741 cgtattagac tatgccttaa tagatgaagg taaggatgca caaaaggcat tgcaagattc
    16801 agtgacactt gcaaaattag cagatcgact tggctttaag cgaatttggt ttacggaaca
    16861 tcataatgta ccagcgtttg cgtgtagtag tccagaactt ttgatgatgc atacattggc
    16921 gcagacaaat cacatacgag ttggctctgg tggtgtgatg ctgccgcact atcgacctta
    16981 taaaattgct gagcatttta gaatgatggc agcgttatat ccaaatcgta ttgatttagg
    17041 tattggtaat aatccaggta ctactatggt aaagcaagct ttagatggaa taaatcctac
    17101 atatgatagt tacgatgaat cgatttcgtt attacgtgat tatcttacaa taaaggataa
    17161 accaagtgcg catacgttag gtgtccaacc acacattgat cattttccag aaatgtggtt
    17221 attaagtagt agcgcaacat ctgccaaaat agctgccgaa ctaggtatag ggctttctgt
    17281 tggaacattt ttgctaccag atataaatgc gatacatgca gcgaaggata acattgatat
    17341 ttacaaaaaa catttccaag catcaacgat taaaatggac gcaaaggtga tggcatctgt
    17401 atttgtcatt gtagctgata acgaagcgga agtagcagca ttacaacatg ccttagatgt
    17461 ttggttatta ggtaaattac aatttgcaga atttgaagat tttccttcag tagacacagc
    17521 acaaaagtat aagcttaatg atcgagacaa agagatgatt caagcacatc aagcacgcat
    17581 cattgcaggt acacaagaaa aggttaaagc acaattagat gatttcattg ctacgtttga
    17641 agttgatgag gtgttagtag caccgcttat tccaggtatt gaacagcgtt gtaaaacatt
    17701 aaaattactc gcggaaattt atttgtagca ttttaaatag aagagaaagg atgaagataa
    17761 gatgaaaaag ttagccaatt atttatgggt agaaaaagta ggagatttgt atgtgtttag
    17821 tatgacacct gaattgcaag atgatattgg gacagtaggt tatgttgaat tcgtaagtcc
    17881 agatgaagtt aaagtggatg atgaaattgt gagtatcgaa gcatcgaaaa cggtcattga
    17941 tgtgcaaacg ccattgtcag gaacgattat tgagcgaaat acaaaagcgg aagaagaacc
    18001 gacaatttta aactctgaaa aaccagaaga aaattggttg ttcaaattgg atgatgtcga
    18061 taaagaagca ttcctagcat taccggaggc ttaaatggaa acgttaaaat caaataaagc
    18121 gagacttgaa tatttaatca atgatatgca tcgagagaga aatgacaatg acgtattggt
    18181 aatgccatct tcatttgaag atttgtggga attatatcga ggcttagcaa atgtcagacc
    18241 ggcattacct gtaagtgatg aatatttagc tgtacaagat gctatgttaa gtgatttgaa
    18301 tcgtcaacat gttacggatt tgaaggattt gaagccgata aaaggtgaca atatctttgt
    18361 ttggcaaggt gatatcacga cgttaaaaat cgatgctatt gttaatgctg caaatagtcg
    18421 ttttctagga tgtatgcaag ctaatcatga ctgcattgat aatattattc atacaaaagc
    18481 gggtgttcaa gttcgacttg attgtgcaga gatcattcga caacaagggc gcaatgaagg
    18541 tgtaggtaaa gccaaaataa cacgtggata taatttgtca gcaaagtata taattcatac
    18601 ggttggtccg caaatacgtc gattgcctgt ttcaaagatg aatcaggact tgttagctaa
    18661 atgttatctt agctgtctta aattggctga tcaacatagt ttaaatcatg tcgctttttg
    18721 ctgtatatct acaggtgtat ttgcttttcc tcaagatgaa gcagcagaaa ttgctgttcg
    18781 aacagtagaa agctatctca aagaaacaaa ttcaacattg aaagtcgtgt tcaatgtatt
    18841 tacagataag gatttacaac tgtataagga ggcatttaac cgtgatgcag agtagtaagt
    18901 ggaatgcaat gtctctgtta atggatgaca agacaaagca ggctgaagta ttgcgtactg
    18961 cgattgatga agcagatgcg atagtgattg gaattggtgc aggcatgtct gcatctgacg
    19021 gatttacata tgtaggagag cgttttacgg aaaatttccc agattttatt gaaaaatatc
    19081 gcttctttga tatgttgcaa gcgagtttac atccttatgg cagttggcaa gagtattggg
    19141 catttgagag tcgttttatt acattaaact atttagatca acctgtaggt cagtcttacc
    19201 tcgctttaaa atccttggtg gaaggtaaac agtaccacat tataactacg aatgcagata
    19261 atgctttcga tgtagctgat tatgatatga ctcatgtatt tcatatacaa ggggagtata
    19321 tactgcaaca gtgtagtcag cattgtcatg ctcaaacgta tcgcaatgat gatttaattc
    19381 gtaaaatggt tgttgcgcaa caagatatgc ttataccttg ggagatgatt ccaagatgtc
    19441 caaaatgtga tgccccaatg gaagtgaata aacgtaaagc ggaagttggg atggttgaag
    19501 atgctgaatt tcatgcgcaa ctacatcgtt ataatgcttt tctagagcaa catcaagatg
    19561 ataaagtgtt gtatttggaa attggaattg gttatactac accacaattt gtgaagcatc
    19621 cttttcagcg tatgacacgt aaaaatgaaa atgcccttta tatgacgatg aataaaaagg
    19681 catatcgcat tccgaattca attcaagaac gtaccataca tttaactgag gatatctcaa
    19741 cattgattac agcagcactc cggaacgaca gcacaacgaa aaataacaac attggagaga
    19801 cagaagatgt acttaataga accgattaga aatggagaat atattactga tggtgcgatt
    19861 gcactcgcta tgcaagttta tgttaaccag catatctttt tagatgaaga tattttattc
    19921 ccttattatt gtgatccaaa agtggaaatt ggacgttttc aaaatactgc tatagaagtg
    19981 aatcaagatt atatagataa acacagtatt caagtagttc gccgagatac tggtggtggc
    20041 gctgtgtatg ttgataaagg tgccgttaat atgtgttgta ttttagaaca agacacttca
    20101 atttatggtg attttcaacg attttatcaa ccagctataa aggcgttgca tacattaggt
    20161 gcaacagatg tggtacaaag cggtagaaat gatttaacat tgaatggtaa aaaagtgtca
    20221 ggcgccgcaa tgacattaat gaataatcgt atttatggcg gttattcgct attacttgat
    20281 gttaattatg aagcaatgga taaagtgtta aagcctaatc gcaaaaagat tgcatcgaaa
    20341 gggattaaat ctgtgcgcgc acgtgttggt catcttagag aagcactgga tgaaaagtat
    20401 cgtgatataa ccattgaaga atttaaaaat ttaatggtga cgcagatttt gggaatcgat
    20461 gacattaaag aggcgaaacg atatgaatta acggatgcag attgggaagc gattgatgaa
    20521 ttagctgata aaaagtataa aaattgggat tggaattatg gcaagtcacc caaatatgaa
    20581 tacaatcgaa gtgaaagatt atcttcaggt acggtagaca taacaatttc tgttgaacaa
    20641 aatcgtatcg cagattgtcg tatttatggg gatttctttg gacaaggtga tataaaagat
    20701 gtggaagaag cattacaagg aacaaaaatg acaagagaag atttaacgca tcagttaaag
    20761 caattagaca tcgtttatta ttttagcaat gttacggtag aagcattagt ggatatgatt
    20821 ttaagttaat attgttattt tatgtatgct gaatcattgg aagtgtttgc ttgctcttga
    20881 aaaggtgaca atagtgtttg gtgaaggttg aacatatgag tggaaattat tgcctttaac
    20941 tattcaaagt atgatatata tatggttttt gtttctaaat gattgggtat ttgaaaatag
    21001 atgagtttaa tattttaagg aatataatga tgtttacttt tat
//
LOCUS       DAKTIP010000025        20891 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAKTIP010000025 DAKTIP010000000
VERSION     DAKTIP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20891
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.025"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            56..550
                     /locus_tag="O0305_000738"
     CDS             56..550
                     /locus_tag="O0305_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCY0273894.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            complement(912..1880)
                     /locus_tag="O0305_000739"
     CDS             complement(912..1880)
                     /locus_tag="O0305_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCY0273895.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVSESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(1935..2975)
                     /locus_tag="O0305_000740"
     CDS             complement(1935..2975)
                     /locus_tag="O0305_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0273896.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPHAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAITAKKAIESGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            complement(3000..4076)
                     /locus_tag="O0305_000741"
     CDS             complement(3000..4076)
                     /locus_tag="O0305_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCY0273897.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            complement(4150..4989)
                     /locus_tag="O0305_000742"
     CDS             complement(4150..4989)
                     /locus_tag="O0305_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0273898.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            complement(4991..6259)
                     /locus_tag="O0305_000743"
     CDS             complement(4991..6259)
                     /locus_tag="O0305_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCY0273899.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYIINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            complement(6262..7533)
                     /locus_tag="O0305_000744"
     CDS             complement(6262..7533)
                     /locus_tag="O0305_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0273900.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            complement(7546..8643)
                     /gene="ugpC"
                     /locus_tag="O0305_000745"
     CDS             complement(7546..8643)
                     /gene="ugpC"
                     /locus_tag="O0305_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCY0273901.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            complement(9026..9604)
                     /locus_tag="O0305_000746"
     CDS             complement(9026..9604)
                     /locus_tag="O0305_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0273902.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGIQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            9813..10439
                     /locus_tag="O0305_000747"
     CDS             9813..10439
                     /locus_tag="O0305_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCY0273903.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            10629..11402
                     /locus_tag="O0305_000748"
     CDS             10629..11402
                     /locus_tag="O0305_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0273904.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGDMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDKKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPKNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKISEH"
     gene            complement(11700..13706)
                     /gene="ggt"
                     /locus_tag="O0305_000749"
     CDS             complement(11700..13706)
                     /gene="ggt"
                     /locus_tag="O0305_000749"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCY0273905.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVLEPFASGIGGGGYLLYYEQSTGSITAFDARETAPEHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDPTTYEASGGSDDTREGTVMGGEVLVIRKQPLPYRQMYDSDGF
                     RLYFNDVQLPLLADQVRWMHDKYWVDESVVRIIFPEVSAHIEDLRSYENAGENYIDIA
                     WLARKYAYQVTLKDDGLYLTDDTYTSVKRNTNAYYRYDRDSITR"
     gene            complement(13744..15519)
                     /locus_tag="O0305_000750"
     CDS             complement(13744..15519)
                     /locus_tag="O0305_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0273906.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(15536..16699)
                     /locus_tag="O0305_000751"
     CDS             complement(15536..16699)
                     /locus_tag="O0305_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273907.1"
                     /translation="MAQLNSKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(16705..18183)
                     /locus_tag="O0305_000752"
     CDS             complement(16705..18183)
                     /locus_tag="O0305_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0273908.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALMVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            18140..19732
                     /locus_tag="O0305_000753"
     CDS             18140..19732
                     /locus_tag="O0305_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0273909.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLNNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVIFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQTFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(19997..>20891)
                     /locus_tag="O0305_000754"
     CDS             complement(19997..>20891)
                     /locus_tag="O0305_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCY0273910.1"
                     /translation="PKEGKKIEIALKDDWSIFNVEEAQISCILNELFKDDENLDRRVL
                     EDLVGAKIINDKKYTNKNLIVANNDWDGFCESLKYKNRFHNNMINLENLAFFLDITTN
                     YYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLNSKWISVLYLSTKEQV
                     SIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIKAGDDGFRKYFVNYQT
                     LKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESILQDGTFNFVFFDQSLF
                     ECVNYERKRVSDVKYTLTKLS"
BASE COUNT         6664 a         3989 c         3182 g         7056 t
ORIGIN      
        1 actttatgtt gtcaaaatca acgcttatac atatgacata atgacggagg acatcatgat
       61 tctatctata ttacttatct ttttctgtat cagacttgtc agcttaaaga tatctattaa
      121 tcactcaaaa caattaaaag cagacggtgc agttgaatat ggcgttaaaa attcgaagtt
      181 tttagcaata acacacgttt taatttatgt attggctggt gtagaggcat ttattaataa
      241 agatacattt agctttgcaa atggtattgg cttagttata ttaatctttg cttatatcat
      301 gttatttatg gttattaaaa ctttaggtgg tatttggaca ttaaaattat tcattttacc
      361 taaccatcct attattaaat ctggattata taaaattaca aaacacccaa attacttctt
      421 aaacatcatt cctgaattaa tcggtgtatt attattaaca cacgcaacat atacaactat
      481 tttattagta ccgtatgcgt atttcttata tgtacgtatt aaacaagaag agaaattaat
      541 gaatatttaa aaagatgaaa gcctgtcatc actttagtct acatcgacgt ccatctttaa
      601 agtgcattct gaaatattat attcagaaac ctttaaagta tgtcgtgtct atgaccattg
      661 tgacaacagg ctttttttta ttatcatata cctcactatt gaaaaggtta tatactcttt
      721 tcaatggtga ggattgttac tgtgaatggg tatgaccatc gctatatact tatataaagt
      781 acaccttctt tacaaacaat gacttgattt aaggtgtgaa atttttggca atggcttggc
      841 gacatatgca tttattcaac actgccacca gtcttacttc agacaaactt cagactaacc
      901 tcgagttcgt attatgccca ccacatgtct gctggcttgt cgtaaatatt aacagatttc
      961 aaagtttgac aagctttttg gaaaccttct tctactgaca taataggatc ttcatgttca
     1021 atacttaata cataatcata tccattaata ataagttgac ttatgatatc tgcccataca
     1081 tatggactat gtccataacc aactgtacgg aatgtccatg ctcttgtcgc aacgttacca
     1141 tatggttgca tatcagttag accatacata tttacatttt cttgattaat atacgtatct
     1201 ttagcatgga agtgatgaat tgcatttgct tggcctaata tgcgaatcgc agcaattggg
     1261 tcaatacctt gccaccatag atgactagga tctaagttag caccgatata ttcatttgta
     1321 gcctcacgta acttcaacat tgtatatggt gtatgcacta aaaatcctgc atgcaactct
     1381 atggcaattt ttacatcttg ctcttttgca aactcagcta aatcttgcca atatggtatc
     1441 aacttttcat tccactgata atcataaatt tcagagtagg ctgttggcca aggtgtaaca
     1501 ggccaattag gctttttagc ggtatcatct gatcctgcaa tgccagaaaa tgtattaaca
     1561 actggcacgt ctaatagatt tgctaaacgg attgttttac gtaacgtttc atcggcttct
     1621 ttcgcttcta tcggatctgg agaaattgga ttgttatgac aactgaaacc acttatttgt
     1681 aagcctctgt ctgtgattga cttcataaat gcttggcgct tgtcttcatt ttctaacaac
     1741 tcatctaact tacaaaattt atctcctggg ttaccacctg ttccaacttc aatcatatcc
     1801 aatccagatt ctgagacata atctaacata tcttcaaaat ttttatcgta aaataatact
     1861 gaaaatacac ctattttcat tgaaaagcac tccttctttt tatattgaga attttgtatc
     1921 atatatgata atcattaaag ttgtatgctc ttgttatcaa gatcgctacg ataaatcgct
     1981 tctataaggg catttacatt gcgtgcttct tccggtttca ccacaatctc ttctatccct
     2041 aaacacgcat tgacaaagtt acgcgcctgt ctctcaccag caatgtcttc gttatgctca
     2101 acattagctt tgctttcaaa aatagttcca aagcggggct catatatttc aaatggaaat
     2161 aaattgatac cgccatcttc tcctgataaa ctaacgtgaa ccttatcttc tttgatattt
     2221 gcagaccacg aacattcaaa ctgcatgctt gctcgattgg caaatgtcat ataactagta
     2281 acatgatcat cgacatcaaa tttagtatga tcaaatgttc cccaatcatt aatttgattc
     2341 ggttgtttgc tcaattgatt atatgttttt cctagcactt catgcggcac catatcttta
     2401 cctagtagcc acaaagataa gtctaacaag tggcaaccat aatcgattaa actaccgcca
     2461 ccttgcaacg ctttattggt aaaaacaccc cagccaggca ctttacgcct acgcatcgct
     2521 tgtacacgtg ctactaaagg tttaccaacc acacctgatt caattgcttt tttagcagta
     2581 attgccacat ctgtgtgacg ataatgatat gcgacagtta ataatttgtg atttttatta
     2641 gccgcttcaa tcatgcgatc acactcttcc gtcgtcatcg ccattggctt ttcacacaat
     2701 acatggacac catggttcaa tgcttctata gaaagatcag catggaattt attaggtgta
     2761 caaatgacca ccgcatcaac aagtttaaac agctcgctag gtgtctcaac tgcatgaggt
     2821 atattaaagc gcttcgcaac atcaatcatc tgcactgtat taatatcttg tactgcaact
     2881 aatgagactg tgtctttgag tttcagcaat gctggaatat gacggtcttg tgcaatacca
     2941 ccaacaccta tcacaccaac ttttaatttt gtcatgatgt gcctccttac cgtatgatgt
     3001 tattcaaagt aaattgcttt gcctgatttt gcagactgat aaatcgcttc aagaattttt
     3061 gtaactacca ttgcttgttc cggtttcaca actggttcag tatcatttac aactgcatca
     3121 atccaagctt ttgcttcttc ttcagcttca tccacttcat taccttcata aaagtcgacg
     3181 cctttgtttt ccaattcaac gtgtttggta taaagtgtac ccatgtcttc accatgaata
     3241 cgtagaccat ctttcatatc agcacctgct ttagttcctg ataatgaaca ttttgcctca
     3301 tccacttcta aagaattaat cgcccaagcg gattctaaaa tgatcgtcgc tccattcttc
     3361 attttaataa atccaaacgc agaatcttca actgtaaatt catctggatt ccatgaaccc
     3421 caagcgtttg ccgcatgatg ctgtttattt aatttatgga atgttgaacc catcactgat
     3481 tctggttcat aattatccat catccataac gttaaatcta aagcgtgtgt accgatatcg
     3541 attaatggtc ctccaccttg agcttcttcg tctagaaaga caccccatgt tggtactgct
     3601 cgacgacgaa tggcatgtgc ctttccgaag taaatgtctc ctaagtcgcc acgttgcgct
     3661 gcttgatgta aaaattgact atctgctcgg aaacgatttt gataacctat tgttaatttt
     3721 ttacctgttg atttagctgt atctatcatt ttttgagctt ctgctgtcgt tttagccatt
     3781 ggtttttcac acatcacatg tttaccagca tgcaaccctg ctacagtaat ttcacaatgc
     3841 gagtcatttg gcgtacaaac atggataaca tcaatcgtgt catcttttaa caatgctttg
     3901 taatcatcat aaacctttgc attgtcagtt ccgtatgctt ctgccgcact cgctgctttc
     3961 gaaatgtcta cgtcacaaaa tgcgatcatt tcaacatttt caactttttg taaacttggc
     4021 atgtgcttgc cattcgcaat accaccacac ccaatgattc caactttaat cgtcatcata
     4081 acgtcctcct tttaatcttt atcgtcatta gtggttcttt atcaattctg ccccactcct
     4141 aatttcaaac taaccttttg tcgcacctgt tgttaaacct gatactaaat agcgttgcaa
     4201 gaacaagaat acgattgcta taggtactgc aatcataatt gcccctgctg caaacactgt
     4261 gaaattattt gcatacttat cattaataaa gttgaacaat ccaactgcta atgtgaattt
     4321 ttcaggactt cttaatagta ttttaggtaa tataaagtcc ataaatggcc ccataaaatt
     4381 gaacaaagca acaactgcta aaatcggctt agctaatgga agcataattt gtaagaaaat
     4441 acgcatatgc cctgcaccat caattttggc agattcatca agttcttttg gaatcgtatc
     4501 gaagtaacct ttcactaaaa aggcattcat cggtattgat ccaccaatat ataccagtgt
     4561 tagtccaaat aaagaatcta ataatccaat tgtatttagc aaaatataga ttgcgaccat
     4621 tgccattaat acagggaaca tttgtaaaat caaaaatgta atcagcccgt atttacgacc
     4681 aacaaagcga tatctagaaa aagcatatgc tgttaacgtg acaaatatca cactaaacag
     4741 tgcatttgca gatgctacga taagcgtatt tttataccaa gtcaggtatt gactactgtc
     4801 atcgaatagt aagaatgcat aatttttaaa tgttgcattg tctggtatca ttttggcacc
     4861 atacaagttc gtacctggat taagggaaat gccaaatgtc cacagtagtg gatataaaat
     4921 gatgacaaac atcatcgcta taaaactgta aataccgatt gcttttaata tgtttttctt
     4981 ctttgtcatc ttataaacct ccctcatctt taaacgtact tgtacgtctg aattgaataa
     5041 atgcgacaat agcaacaata aatccaataa ttaatgacac aactgcaccc atgttgaagt
     5101 tgttaaactc aaatgtcaga ttatacaccc aagatatcaa gatatctgta ctaccagcat
     5161 tctgccctga cactggtgga ccgcctttat taaatagata aataagatta aaattattga
     5221 aattacctgc atattgcata attaacaatg gcgctgtggc gtaaatgaca tgcgggaatg
     5281 tgatgtttct aaacttttgc caactagacg caccatccat atctgctgct tcgtaccaat
     5341 ctgatgaaat actttgcagt actccagtga acagtgcaaa gacaaatggg aatccaagcc
     5401 atacttgaat gccgattaat gccacttttg cccaaaacgg atcacttaac catgctggtg
     5461 ctacacctaa taaaggttgc aaaatatcat tatttatcgc accaaattca tcattaaata
     5521 acgctacaaa tattaaaatt gtcacaaatg atggtacagc ccaaggtagg attaacacag
     5581 tacggataaa tttcttacct ttgacgacag ggtgatttac aataattgcc aaaaacagcc
     5641 ctaatgcaat ttgaagtgtc gttgcaacaa gcgtccatac taatgtccaa gtaataacac
     5701 tgaaaaatgt tttacgccaa acgccaattg tgaataacgt tttaaagtta tctaaaccaa
     5761 cccattctaa tgtgtgtctc ggaggcgcgt tgtataaatt gtaatttgta aatgctactc
     5821 caaacataaa tattaatgga aatacaacta caaatatcaa taagaatgta cctggtgaga
     5881 ttagtaagta tgggaacgtc ttgtcccatg ttgccaccat acgcgccttc ggatccttta
     5941 tttcctcatt gcgattaaat cgttcagcat tacgatatgc atctaaaata ttaatgatat
     6001 atagcatgat tgcgaaagca acgagtaaga tagaaataat accttgtgca agtaagacac
     6061 gagaatcgtc taacttaggt actgtcccta atgtgaacaa tccccaaaaa ccaatattta
     6121 aaaagctata aaaaacagaa ataaaactga tgaaaaagat aaaaaatatc gtccctttaa
     6181 tgggtctttt attataaaac tgtcccaaac caggtataac agataataat gccgctaatt
     6241 tagggttacg tttcgtcata actaatctcc tttcttatca ttttgtgatg gatgaagaat
     6301 cttaatattt tgcgttatat cattcgtcgc ctcatctaac gcttgtttag gattcttacc
     6361 atttgaaata aatatgcttg cattgcccat cggttcccaa acttgtcgca tttcaggaat
     6421 attaggcatc ggttcagcat gacgtgcttg cttttcaaac acttttaaat ttggattaga
     6481 tgatttcacg tcaacacgtc cagtaatttc gctcatttca tctgtatatt tttgtaatgt
     6541 atctttacta gtgatataca gcattaaatc tttagcccaa tacttatgtt tactatattc
     6601 agataaatac caaccacgta cacctagaaa tggtttcata ggtttgccac catctgtagg
     6661 taatgttgtt actcctaaat ctttaccaaa cgtttcttga tattcattaa tgttccacgg
     6721 tccagtgaca aattgtccta cttttccttc tttaaaaaga ccaatcatga catcatgtgt
     6781 tgctgcctta ggaagatacc ctttgtcgta ccatttttgt aatcgttcag cattcttgac
     6841 gacatgtttt gaatttagtc ctagctgatg aatatcatat tcactgccat ttttcttgaa
     6901 aatataatca tcattgccga ataaaaacgg ataattaaaa tagaaatttt tagcatcaaa
     6961 taacataccg tattgtttct ttttactatc agttagtttg gcagcattag cttcaacttc
     7021 ttctaacgtt tgcggtgcat ttttcactaa ttttttatta taaaaaagtg cggttgtttc
     7081 aacgatagct ggcaatgcta gttgcttatt gtcataattc atcgctttaa gtgcttgctt
     7141 attgaaacct ttcaactcat cttttgataa tttgatttca gcagctaagc cttgtagata
     7201 ggcacttcca gtattatcat gtgctaagaa aaagatatct ggaccttttc ctgcaggagc
     7261 gtctagcgaa atattttcta gttgatcatt ttgaccaata tttacaagct ttactttgat
     7321 gccagttttt ttagtatatt gatccgtaat ttttttataa aacgccattt gcttgtcgcc
     7381 atccacccac atcgtaagtt ggttaggctt gtctttagaa ttatctttat tcaatgcttt
     7441 atcaatatct tctttcgaac gattagggcc acatgcagtt acgattaata acattaccac
     7501 ggctaacgtg atacatttta aaattttaga catgaatact cccccttaga cgatacgatt
     7561 tcctgttttt tcatcaaaaa agtgacactt attcatatca aatgctagtg taatcttgtc
     7621 gttcgccatc acttgagttc ttgaatctaa tttagaaatc aattccattc cttggaatgt
     7681 gctatgtacc ataatttctg aacctaacag ttcggataca actacttcag attcaaattg
     7741 tgtctctgga gaagtttgaa taaatattgg ttcttcatga atgtcttcag ctcgaatacc
     7801 taaaataatt tccttgtcta agtagccagc agcttttaac ttttcaaatt ttttattatg
     7861 taatttaaaa tggtgtgttc ctaccttcaa tccgtccatt tcaactgtag cattcaacat
     7921 attcattgct ggtgagccga taaattgcgc cacaaatatg caattagggg catcatatat
     7981 ttctctaggt gtgccgactt gcataatgtc gccatctttc aaaacaacaa ttcgactagc
     8041 catcgtcaat gcttcagttt gatcatgtgt aacataaatt gtcgtagtat taagtcgctt
     8101 atgtaatttc aatatttctg tgcgcatttg tactcgaagc ttcgcatcaa gattcgataa
     8161 tggttcatcc attaaaaaga ctttcgcatc cctaacaata gctctgccca aagcaacacg
     8221 ttgacgctgt ccgccagata acgcttttgg tttacgacca agatactcag ttaatcctaa
     8281 tatttcagct gcttcattaa ctttttgttc aatctctttt ttatttactt tacgtagctt
     8341 tagcccaaat gccatatttt caaaaacagt catatgtgga tataatgcat agttttgaaa
     8401 taccatcgca atatctctat tctttggttc aacatcgttc atgcgttccc catcaatata
     8461 aaaatctcca gatgtgatag actctagtcc agcaaccatt cgtaatgttg ttgatttacc
     8521 acatcccgat ggtccaacaa atacaatgaa ttctttgtca gtaatatgta gattaaaatc
     8581 tttcactaca gtattgttgt tatcatacgt ctttttaata tgctctaact ttagttctgc
     8641 catgttatgc ctcccatact ttgtttacag tttgattgtg caatacactt cgtgaaaacg
     8701 tttgcacaaa tacttacata aatcatatat tcagtcatta ttaatgtcaa tcataaatta
     8761 taaacgttta cattactgtc agaattaaac ttttatacag aaaattgata tagtgcacaa
     8821 ctattttcta gtgtgacaat aatgtttttt atgtgcgata tttcactgcc aatgctaaca
     8881 accttgtctt acattttaag taatatctaa atcaaacaag tatgacttag cattacgatt
     8941 ttcattgaaa aaagcaacca ttatgtaagt atcatactca cacaatgatt gcttaatttt
     9001 ttagactgcg ggcacccttc tataactaca aatcatatag gctacgttcc ccatcaggta
     9061 actgatcgat aaacggtttt ggatcttctg catatgcatt acctacgcca cccttcattc
     9121 tttgaaaatg taaatgtgcg cctgtcgttt gtatacctgt attgcctgaa tatgcaataa
     9181 tatcacctgc tttgactcga tcacctactt tgacattata tttgtctaag tgtagatacc
     9241 actggtgata ttctccattg tcttcggcaa tctgtaatac cttgccgccc aattcattat
     9301 taaagatacg tgttacttta ccattcgtcg gcgctttaat tggtgtacct tttggcaatg
     9361 caaaatcaat gccataatgc ttaccatcaa atggtgaatg ttgatattta ccaaaagtct
     9421 cagtaatccg actccctcta aaaaagtctt cccattttga gtgtccttgt cctttatgat
     9481 tttgtgtcaa tttatcggta tattggttaa accatttttg aatgtcatct atataaataa
     9541 tcgtaaataa tagtgaaaga aaaatgataa ttgttgccaa aatacgtttt ggtcgctttg
     9601 tcatagtggc atccatcctt catatatttt gggtattaag tatatattct aaatcaacta
     9661 tttcataatt aaatgcactc atactttagt agtactgcca aatatagatg aagaaaatga
     9721 tggtaatttg aatattttga ttgatattaa attttatatc gagtataata attatactta
     9781 agaaaataac agaaacagag gagaattttc aaatggcaaa agtattatat attacagcac
     9841 acccttttaa tgaactggta tcaaattcta tggcggctgg taaagcattt atcgaaacat
     9901 atcaacaaca acatcctgat gatgaagtga aacatattga tttatttgaa acttatattc
     9961 cagttattga caaagatgta ttaactggtt ggggaaaaat gagtaatggc gaaacgttaa
    10021 ctgatgatga acaaatgaaa gtttcaagat tgagtgacat tttagaagaa tttttaagtg
    10081 cagataaata tgtatttgta acgcctatgt ggaacctttc tttcccacct gtagttaaag
    10141 catatattga tgctatttca attgcaggta aaacttttaa atattctgct gaaggaccac
    10201 aaggtttact aactgacaaa aaagtattac acattcaatc acgtggtgga tattatactg
    10261 aaggacctgc tgctgatttt gaaatgggtg accgttattt aagaacaatt atgacattct
    10321 taggtgtgcc atcatatgaa acaatcatta ttgaaggaca caatgctgaa cctcataaga
    10381 ctgaagaaat taaagcaact agtattaata atgctgaaaa attagcaaca actttttaaa
    10441 tttttagtta cactgtgtga cgtctcaaac gtcatgcagt tttttattgt atctcttttc
    10501 cgctttttat aaatatctat tattttcata aatttaaaat ttgatattca ctttaatact
    10561 gatgtaaata tttttcaaaa tatattatta tagtgttaat gaagtatttt gagaaacggt
    10621 gggaatgtat gaaagcacat aaaattttct ggttaaactt agcagcaatt attattattt
    10681 caattgtagt cagtggcgat atgtttttgg caatgaagtg ggaacaaatt catttgaaag
    10741 atggtcttaa aaaagtacta agtacgtatc ctattaaaaa cttagaaacg ctttatgaaa
    10801 ttgatggtca cgataatccc cattatgaaa ataatgatca agacacatgg tatatagaat
    10861 cctcttattc agttgttgga tcagatgagc ttttaaaaga agaccgtatg ttgttaaagg
    10921 tagataagaa cacacataaa ataactggtg aatatgacac aactacaaac gataaaaaaa
    10981 atgctactga ctcgacatac aaaagttatc cagtaaaagt agttaataat aaaatcgttt
    11041 tcacaaaaga cgtaaaagat cccgcgctga aacaaaaaat cgaaaacaat caatttttga
    11101 ttcaaagtgg tgatttaaca agcattttaa atagcaatga tttgaaagtc acacatgatc
    11161 ctaccactga ttattataat ttatctggta agttgtcgaa cgataatcca aacgttaaac
    11221 aattaaaacg tagatataat attcctaaaa acgcatcaac aaaggtggaa ttaaagggaa
    11281 tgagtgattt aaaaggcaat aatcatcaag atcagaaact ttatttttat ttttcaagtc
    11341 ctggaaaaga ccaaatcatt tataaagaaa gccttactta taataaaata agtgaacatt
    11401 aatacttatg ctgtaattat agaaacatcc aaatcatcta ttaaaatcct atattataaa
    11461 aacacctcac ataactcgtt caactgtacc aaaccacatt acattagatt ttaggctaac
    11521 tattgtgatg tacatcaaaa acgaatttgt gaggcgttgt atattttaca aaggtgacta
    11581 gcgtttcgta tagcatttcc aacattacta cactcaagcg tcacgctaaa gttcgaaatc
    11641 gaatcctttc attcaacaaa agctcatatc cactacaaac ttcatatcaa gcgtataaac
    11701 tatcttgtga tactatctcg atcatatcta tagtatgcat ttgtgttccg tttcactgaa
    11761 gtatatgtat catcagttaa gtataaaccg tcatccttca atgttacttg ataagcatat
    11821 ttccgtgcta accaggcaat atctatataa ttttctcctg cgttttcata acttcttaaa
    11881 tcttcaatat gtgcactaac ttcagggaaa atgattctaa caacactttc atcaacccaa
    11941 tatttgtcat gcatccatcg cacttgatct gccaataaag gtaactgcac atcattgaaa
    12001 tatagacgaa agccgtcact atcatacatt tgccgatatg gtaatggctg ttttctaatc
    12061 actaacacct cgccacccat tacggtgcct tctctagtat catcacttcc acccgaagct
    12121 tcatacgttg ttgggtcaac ctgtagtcca tgtacatctc caatataagc atctggttta
    12181 tgttccattg catgtccatg tgcaatcaat gctaatattg tagattgtga aaattgaggc
    12241 tcccattcaa tgcgattagg atggctacta taaattctag gttcatctat agcctgctga
    12301 atatccatgc caaacactaa tacattgatt aatgtttgcg caacactagc aatgatactt
    12361 atggcaccag gtgcacctac tgttaatatt ggcttcccgt gatacatcac aatcgttgga
    12421 gccatgttac ttagtggtcg tttatatggt gcaatttcgt taataccacc atctactaca
    12481 tcaaagccat ccattgtcgt attcaataac acaccgtagc ctggaatcgt gatacctgaa
    12541 ccataaatca taccaattga tgtcgtaaat gaagcaatat taccttcctt atcaatcaca
    12601 caaaaatgag ttgtttcggt atgattttct tcaacatctg tatgactaat gcaatcagac
    12661 acaacaccat gctctatatc aatatttgct ttattgctat caatgagcgt actgcgtgct
    12721 tttaaataat catcatcaat taatgactgt acaggcacct catgaaaatt atcatccgcc
    12781 aagtattgcg cacgatcact atatgctaaa tgcatcgctt gtatcaaatg atgcaagtaa
    12841 tcaacagatc ttggacccat agatggtaaa tcgacatgtt ctaataactt caatatttga
    12901 attaccgtga taccgccaga actagatggt cccattgaat aaatgtcata gtctttaaat
    12961 gttgcactga ttggcgcttt aatctgaatg tcatatttgg ctagatcctc taaagtgatt
    13021 gtcccaccac atgctttgac aacattgact aattgtttcg caatgtcacc tttataaaat
    13081 gcattaaacc cttgttctct taatatttga aatgtcttac ctaattcggg ttgtacaatc
    13141 caatcacctt cacgccaata ttgattttca tgcgtaaata cttgtgccgt ttcatgatac
    13201 tttgtcaatc gtgcgtgttg ctggcgcgaa tatttttcag tagcccaatt ggctgcatga
    13261 ccttcaatgg ctagttcaat tgcaggatta attaaatctt ccaatgacaa tttagcataa
    13321 cgcttgtgaa tataatcaaa cagctttgga attgctggca cagcgacagt tttaccatgt
    13381 gtagtcatat caaaaaatga tttatattcg cctgaatcat ctagataaaa ttgtttgtct
    13441 acatgttcag gtgctgtctc acgtgcatca aacgcagtta tactgccagt actttgctca
    13501 taatatagca aatacccgcc accaccaata cctgatgcaa atggttctaa cacattcaat
    13561 gccagttgaa ttgcaatcac tgcatccatg gcgttgccac cttgatctaa tacatcctta
    13621 ccaattttag ccgcaagagg atgtgatacg gaaattaacc cttctttaga tgtttttgtc
    13681 tgtttgtcat ttaagttaat gaccatacta tatcctccta ctttctgtta aatatttaaa
    13741 acattattga ttaatggctt tttctacttt ttctaaatct tgacgttgct cgttaccagt
    13801 atcgacaagt ggtgtaatcg gtgatgcaat tttaaattta tcgccacgat aaaacttaat
    13861 aaattgatcc tgatctatcg cattaactac tgcttgtctc aagtttggat gcgtcttaaa
    13921 tatacctttt ttaatattta gcattaaaaa gactgacttg cgtccatttt tgcgaataat
    13981 gcttaaattt ttatccgact taattaaatc aaaatgtttt tgattcacat ctgccaacat
    14041 atcaattgaa tgatttctaa gttctgacaa tgcattattc gggtcaccat taaacttcaa
    14101 tgtaatattt ttaattttag ctggtccata actacctttt tctgtttcgt tgaatcctgg
    14161 attacgttga aacgttgctt gatatgcatt tttctgtgtc ataatgtatg cgccacttgc
    14221 atacagcgca tttttcccat ctgaatttgc aggaattgta ctgctatccc catatccttt
    14281 tggatattct tgatttactt gattaacaaa ttttttagat aaaatgcctg ccgaagagtg
    14341 tgttaagtaa tttacctctc gaggcatcga ttgatctgtc gtaattttaa caatttgata
    14401 aataccgtct ttattattta ctttttgacc atctgtcgtt aacgatttga cgttataagc
    14461 tttaatcaac ttatcataga ttgatttatc gtccttgtct ttctctttac gcaactgatc
    14521 gatgtcctca tcttttaata tcttgatgtc atttatatgt ttgtgcatat tgtaagtatt
    14581 attgttaggc acagactttt tatcacgtgc tctatctaaa gaaaacttaa catcttcagc
    14641 cgatacacgc tctccagtat tacgtgcttg tccattgacc actttcgcaa aataatcatc
    14701 atctcttaac aagaaataaa atgctttatt gtccttattc acagcataat catgacttaa
    14761 cgaacctttc gttgttaaat gatcattttc atctaataat aataaccttg tgtacatatt
    14821 catattaatt gaatatactg acggcgcaat tgaacgtatt ggatccaatg taggaatttc
    14881 accatcttgt tgtgtcatca caagtggccg cgtatctcgt tctctactat tgttgtaatc
    14941 aaattgttgc catattaatg cacgtgaatt tggcaatcca acactatttt tatctaacac
    15001 tttattgtca tatactaaat tcttttttga tccatataaa ggcgccatat accctttatc
    15061 aaatacaact tcatcttcaa tttgcttata tgtttgttta acatctgctt cattttgagt
    15121 agaagcttta tttaacaact ggtctacatg tttatctttc aataaactat ttgatcctgt
    15181 agaactaaat aatgccgtca tagcatagtt cgggtcacca aacactgtca tccagtcatc
    15241 aatttggata tcataattgc cggcttgacg ttgtgtacga tagctaccat aatctggttg
    15301 gatattcatc ttcacgttaa atcctgcatt ttccaattga tctttaacga tattcatatc
    15361 attttcataa cttgcttgtc ctaggaaatg tattgttggt cgctcgcctt tcacttcaac
    15421 tttcgatgac ttttgagcca cttctgattt cgtagggaca ccacaaccac ttaataccaa
    15481 cgctaaaact ataattgcga tactaatgat tttcttcaca tctatcccta cctttttaat
    15541 gaattcttgg atctagtgca tcacgcactg catcacctat aaaattaaat gctaaaacga
    15601 cgaacataat acaaacacca ggtacaatag ctaaattact gtgcgtttcc aagtagttac
    15661 taccggtacg taaaatgttg ccccattcag ctacatcagg tgcaacacca agtcctagga
    15721 aacttaaact acttgttgtt aatacaacca cacctatatt taatgaaaaa cgtacaatca
    15781 taggcgcaat cgcattcggt aaaatataac gccatatgat attccaagtg ttttcaccag
    15841 tgatacgtgc tgcatctaca tattccatgc gtttaatttc taaaacactg gcacgcattg
    15901 tccgtgcaaa tgatggtata ttaccgatac ttaaagcaat aattaaattt ggaatacttg
    15961 ctccaaatga tgcaataatt gccaccgcta acaataatga tggaattgca aacactacat
    16021 ctaaaattcg cattattaaa ttatcaatat gattaaaata acctgcgata gtgcctagta
    16081 acacaccaaa aataactgca ataactactg aaataattga aattgaaaat gtcagcttcg
    16141 ttcctacaac tacgcgtgta aataagtctc taccgaaatc atcagtacca aacggatagg
    16201 ctagactcgg tccatgtaac agtgcattga actgattttt agtagccaat gtcgtatcaa
    16261 atgtaaattg tgacacaatt gataatgtca gcatgtagac taaaataagt aacccgataa
    16321 tcgcaatacg atgtctagta gtttttcgta taaacgattc ccacccgtta taactatgta
    16381 tttgcgatgt acgttggtaa cgtctaatac ttacaaacat taataatgta aatacgttgc
    16441 ctgttaatgt catcaacaat aacaacactt cgacgatacg tcgccatagg tcatgatgct
    16501 tccatgtttg ttccgttgtt aaaataataa ttaaaatgat ggttaaaacg attagcaatg
    16561 tttcagcaat atagaacgta tcggccacat aacctttaaa aagatttaat gcactcgtta
    16621 atataactaa aatataagtt gctatggcgt aacttgcgaa taattttaag gaagctatct
    16681 ttgaattaag ttgtgccata tgcctcactt cctttcgttg atttcactac gtaattttgg
    16741 atcgattaaa gcataaaata tatcaataat taagtttgct aaagatatta caattgatat
    16801 atatacgacc ccacccatga ctgctggaat atcaggtatt agttgttttt ggacgatata
    16861 acgcccgata ccattaatgt taaatacttg ttccgtcact gctgaaccgc ctagtaactc
    16921 tgccactaga agaccaacta acgttacaat tggaataatg gcatttttca aaatatgttt
    16981 aataacaact tgtgtcgtcg ataatccttt tgcataagca gttaaaacat aatcgctgcg
    17041 cattacttca agtacagaag accttgtcat acgcgtgata gaagcagcaa tacttgttcc
    17101 aatgacaagt acaggtaaaa tcaacgatat tggatgttct ggcatataag atggtggcaa
    17161 aatatccaat ttcaatgaga acgctaaaat gaataatagc ccttgccaga aacttggaat
    17221 agataaacca attaatgcaa ttatcattaa cgtgatatca agccaactat ttcgcttcat
    17281 cgcactgata ataccaattg gtattgcaat aattaatgcc accattagcg ctaatactgc
    17341 gacaattatt gtaattggaa ttctttcgcc aactgcttta gtcacaacct cattcccttt
    17401 gtaagtcgta cctaagtcaa aggtaaaaac acccttgatg gtatcccaca attgaataaa
    17461 ataaggttcg ttaagatgat gtaatacatt gaattgatgt atctgtgcct ttgttgcatt
    17521 ttgtcccagt atgctataag ccgcatcaag cggtgaaaaa tacagaatgg taaacacact
    17581 gacaataaca ccaatgatga caatcacagc catgacaatt cgttcaaaaa tatatctaac
    17641 taatggctgt aaataaaaag tcaataagat gaacatcggc aaggccaata tcactttgat
    17701 catgatgaac ttatgaaata atacattttc aaagtatgtt gaaaaatgtg cttgttcaat
    17761 attctttgaa ctcgtattag aactttgtgc cttgaatatt tttaatgctt ctttatgtat
    17821 ttgtgtggat gacttttgct gcgataaata tttatatttt tgatgtaacg cctgttcaat
    17881 ttctgaaatt tcagaattat tagcgtaaaa atttttcctc ttagcagaaa agaaaaactt
    17941 tatcactgca tataaaaata ttggcaagct taataccgat aatacaaact tgtaaaatgc
    18001 cttgtgatac aacgacttaa aagattctaa ataagtaatt atgataatca ctccttgtaa
    18061 aataatcttg agtagattac tatgatatac aaaagtatag aataaattta cacatttgtg
    18121 aatagggagg cacaacatca tgtcaaattt attagaagtc aacagtctga atgtacaatt
    18181 caattatgat gaaactacag ttcaagcggt aaaaaacgtc tctttcgaat tacgaaaaaa
    18241 acatatccta ggtattgttg gtgaatcagg atcaggaaaa agtattaccg ctaaatctat
    18301 tttagggcta ctaccagatt atccagatca cacattaaca ggagaaatta tttttaatgg
    18361 gcaatcgtta aataatttat caacttcagc gttacaacaa attcgaggta aggatatttc
    18421 aatgattttt caagatccac tctcttcgtt gaatccaaga ttaacgattg gcaaacaaat
    18481 tacagaagta atatttcaac ataaacgtgt atctaaatct gaagcaaagt cgatgacaat
    18541 agacatttta gaaaaagtag gtataaaaca tgcaactcga caatttgatg cttatccaca
    18601 tgaactttct ggtggtatgc gtcaacgtgt catgatagca atggcattga ttttaaagcc
    18661 acaaatttta atcgcagatg aaccaacaac ggcattagat gccagtacac aaaatcaatt
    18721 actgcagtta atgaagtccc tttatgagta cacagaaaca tctattattt ttatcactca
    18781 cgatttaggc gctgtgtatc aattttgcga cgatgtgatt gtaatgaaag atggaagtgt
    18841 cgttgaaagt ggcacggttg aaagtatttt taaatcgcca caacatacct atacaaaacg
    18901 cttaatagat gcgattcctg atattcatca aacgcgtccg ccaagaccgt taaacaatga
    18961 tattttatta aaattcgatc gcgtgagcgt ggattacaca tcaccgagtg gcagcctata
    19021 ccgagcagtt aatgatatta acttggctat tagaaaaggc gaaacattag gcattgtcgg
    19081 tgaatcaggg tcagggaaat cgacattagc taagacggtc gtcggtctaa aggaagtgtc
    19141 agaaggcttt atttggtata acgaattacc attaagttta tttaaagatg atgaattgaa
    19201 atctttacga caagagatac aaatgatttt tcaagatcca ttcgcatcta ttaatccaag
    19261 atttaaagtc attgatgtga ttaaacgacc actaatcatt catgggaaag tcaaagataa
    19321 tgatgacatt attaaaactg tcgtatcgtt gttagaaaag gttggcctag atcaaacttt
    19381 cttatatcgc tatccacacg aattatctgg tgggcaacgt cagcgtgtaa gtatcgcgag
    19441 agcacttgct gttgaaccta aagtgattgt ttgcgacgag gcagtgtccg ctttagacgt
    19501 ttcaattcaa aaagatatca tcgagttatt aaaacaatta cagttagact tcggcatcac
    19561 ttatttattc atcacacatg acatgggtgt tatcaatgaa atatgtgatc gcgttgcagt
    19621 tatgaaaaat ggcgaaatcg ttgaactgaa taacacagaa gatattatca aacatccgca
    19681 gtcagactat gcaaagcaac ttatttcaga agtagcagtt attgctaaat aaaagtcatg
    19741 cgttgtgcaa ctttatcact gtatggtctg aaataaattg cgcgacttct gatgtgttat
    19801 gttagatgac tttgtcatac taactatatt gtgctaagtt agggctcggc accatatcaa
    19861 gttttaggtg ctttgccatg atttaagagt cacccccata ctttgggcat tttaacgcca
    19921 gaataaatcc cccgccacta tgtgaagtgc gggggattat ttatatttta ttagaatatt
    19981 cagatttttg agtgtgtcaa cttagcttag tcaatgtata tttaacgtca cttactcttt
    20041 ttctttcata attaacacat tcaaataaac tttgatcaaa aaacacaaag ttaaaagtac
    20101 catcttgtaa tatgctctca tacattatcc cgtcatattt aaggcttcga atataatcag
    20161 ctaaatattg aaatggcaaa taatctattc cttgttcatc gcttggattt gttattcctt
    20221 tatgaatctt ttttaatgtt tggtaattta caaaatactt tctaaatcca tcatcgccag
    20281 ctttgattgc attactagtt aaattagtta aattcgcaat tttcaatttc tcttttgtca
    20341 cgtttttttg taacttaacc ttacctatat aaataatgtc attatgctta ggtttaactt
    20401 cttctatact gacctgttct tttgtactaa ggtataatac gcttatccat ttagaattca
    20461 atcttcctgc cgttgcaaat ccctttggtg gtgacattag ttcacttttc tctgtaatga
    20521 acttaactat tctagatcta tataatggtt caaatctttc tctaaattcc tcaatactat
    20581 agtaattagt agtgatatcg agaaagaacg ctaaattctc taaattgatc atattattat
    20641 gaaatctatt tttatacttc aagctctcac aaaatccatc ccagtcatta tttgctacaa
    20701 ttagattttt atttgtatat tttttatcgt ttatgatttt agcgcctact aaatcttcca
    20761 acactcgtct atctaaattt tcatcatctt taaaaagttc atttaaaata caacttattt
    20821 gagcttcctc aacattaaat atactccagt cgtcttttaa tgctatttca atctttttac
    20881 cttcttttgg g
//
LOCUS       DAKTIP010000026        20650 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAKTIP010000026 DAKTIP010000000
VERSION     DAKTIP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 20650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20650
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.026"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            687..1982
                     /gene="purB"
                     /locus_tag="O0305_000755"
     CDS             687..1982
                     /gene="purB"
                     /locus_tag="O0305_000755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCY0273911.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
     gene            2091..2393
                     /locus_tag="O0305_000756"
     CDS             2091..2393
                     /locus_tag="O0305_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273912.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2565..3257
                     /locus_tag="O0305_000757"
     CDS             2565..3257
                     /locus_tag="O0305_000757"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCY0273913.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKMAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3254..5446
                     /gene="pcrA"
                     /locus_tag="O0305_000758"
     CDS             3254..5446
                     /gene="pcrA"
                     /locus_tag="O0305_000758"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCY0273914.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVLSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5450..7453
                     /gene="ligA"
                     /locus_tag="O0305_000759"
     CDS             5450..7453
                     /gene="ligA"
                     /locus_tag="O0305_000759"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCY0273915.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7466..8665
                     /locus_tag="O0305_000760"
     CDS             7466..8665
                     /locus_tag="O0305_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCY0273916.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGSTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8754..10292)
                     /gene="putP"
                     /locus_tag="O0305_000761"
     CDS             complement(8754..10292)
                     /gene="putP"
                     /locus_tag="O0305_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCY0273917.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIIRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10659..10961
                     /gene="gatC"
                     /locus_tag="O0305_000762"
     CDS             10659..10961
                     /gene="gatC"
                     /locus_tag="O0305_000762"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCY0273918.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10963..12420
                     /gene="gatA"
                     /locus_tag="O0305_000763"
     CDS             10963..12420
                     /gene="gatA"
                     /locus_tag="O0305_000763"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCY0273919.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12433..13860
                     /gene="gatB"
                     /locus_tag="O0305_000764"
     CDS             12433..13860
                     /gene="gatB"
                     /locus_tag="O0305_000764"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCY0273920.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13900..14046
                     /locus_tag="O0305_000765"
     CDS             13900..14046
                     /locus_tag="O0305_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273921.1"
                     /translation="MIADLDAIEGLFISIEVKLLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            14629..15576
                     /locus_tag="O0305_000766"
     CDS             14629..15576
                     /locus_tag="O0305_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0273922.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            15657..17018
                     /gene="rlmD"
                     /locus_tag="O0305_000767"
     CDS             15657..17018
                     /gene="rlmD"
                     /locus_tag="O0305_000767"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCY0273923.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPLAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            17188..17718
                     /locus_tag="O0305_000768"
     CDS             17188..17718
                     /locus_tag="O0305_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0273924.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            17965..19035
                     /gene="dinB"
                     /locus_tag="O0305_000769"
     CDS             17965..19035
                     /gene="dinB"
                     /locus_tag="O0305_000769"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCY0273925.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(19103..19657)
                     /locus_tag="O0305_000770"
     CDS             complement(19103..19657)
                     /locus_tag="O0305_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCY0273926.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKIHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            19872..19964
                     /locus_tag="O0305_000771"
     CDS             19872..19964
                     /locus_tag="O0305_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273927.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(20046..20546)
                     /gene="ftnA"
                     /locus_tag="O0305_000772"
     CDS             complement(20046..20546)
                     /gene="ftnA"
                     /locus_tag="O0305_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCY0273928.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
BASE COUNT         7625 a         2973 c         3782 g         6270 t
ORIGIN      
        1 atcgaaagtt tcatgaaaat atattagtcg attttgaaag aagcgtagtc aatttatgtc
       61 tttctataaa aatttactat taaaaaaatc tctgcgtatt tttctataat cagtttcgta
      121 tttatacgat tctggagttc aatattaatg tcttatattt aaaatgccac acctatgcaa
      181 tagaatatat attctttttg tgagtagaaa atttgttata ttagaagcga ataattttaa
      241 aacatattat gaaatgtaag aagaaatatt atgaaactag gaaaagtaga ttgttgaaaa
      301 ttccatacaa taggagaagt aaagtgaata aattctggta ttactaaagc tattgattgt
      361 cctaattgta ataaaaagtt ggatagtgta gttgagggga gaaaaactaa aatgcaaaaa
      421 tatattctca atggatcatt ataaatacca attagattaa agtattgcga acatttacaa
      481 ttagaaatca gtattaatct gccatagata attgtattaa tgtaaaaaat aaaatagaat
      541 gagaatatac ggaaattttt tatacattta taaccaattc tgttaaactg tgaagagtag
      601 agttaaaaac gaactttttg tttattttta ttaaaaaatg ttctgttttt gaaaaatcga
      661 gtatacatac gataggagtt aaaaaaatga ttgaacgcta ttctagagaa gaaatgtcta
      721 atatttggac tgatcaaaat cgctatgaag catggttaga agtggaaatt ttagcatgtg
      781 aagcatggag tgaattagga catataccga aagctgacgt acaaaaaatt cgtcaaaacg
      841 caaaggtaaa tgtcgaacgt gcacaagaaa ttgaacaaga aacgcgccat gatgttgtag
      901 cctttactag acaagtttct gaaacgctag gtgaagaacg taagtgggta cattatggtt
      961 taacttctac tgatgttgta gatacagctt taagtttcgt tattaaacaa gcaaatgaca
     1021 ttattgaaaa agatttagaa agatttatcg acgttttagc tgaaaaagca aaaaattata
     1081 aatatacatt gatgatggga cgtacacatg gagtgcatgc agaaccaact acatttggtg
     1141 tcaaaatggc attgtggtac acggaaatgc aacgtaactt acaacgtttc aaacaagtaa
     1201 gagaagaaat tgaagtaggg aaaatgagtg gtgcagtagg tacttttgct aacattcctc
     1261 ctgaaataga aagttatgtg tgtaaacatt taggtattgg cacagcgcct gtatcaacgc
     1321 aaacattgca acgcgatcga catgcatact atattgcgac attagcatta atcgcgacat
     1381 cactagaaaa atttgccgtt gaaatacgta accttcaaaa aaccgaaacg agagaagttg
     1441 aagaagcatt tgcaaaaggc caaaaaggtt catctgcaat gcctcataaa cgaaatccaa
     1501 ttggttctga aaatatcaca ggtatttcaa gagtcattcg tggttatatt actacagctt
     1561 atgagaatgt gccattatgg catgaaagag atatttctca ttcttctgct gagcgtatta
     1621 tgttaccaga tgttacaata gcactagatt atgcattgaa tcgtttcaca aatattgtgg
     1681 accgtttaac tgtatttgaa gataatatgc gtaataacat agataaaaca tttggtctga
     1741 ttttctcaca acgtgtctta ttagcattaa ttaataaagg tatggttcgt gaagaagcat
     1801 acgacaaagt acaaccaaaa gcgatgatat cttgggaaac gaaaacaccg ttccgtgaat
     1861 taatcgaaca agatgagtct attacaagtg ttttaacaaa agaagaattg gatgaatgtt
     1921 tcgatcctaa acatcatttg aatcaagttg acactatttt cgaacgcgct ggattagcat
     1981 aaaattaaat aaagtaacga ttcagattta ataaaatata aatcgctaca ctttaataga
     2041 ttaatgcgtt aaaatgacat aaagaataaa attatagggg ttgtttcgat atgcaaattg
     2101 aaaaattacg aggtgcagcg ttagatgaat tgtttgatgc aatactaacg ttagaaaata
     2161 gagaagaatg ttaccaattt ttcgatgatt tgtgtactgt aaatgaaatt caatcactgt
     2221 ctcaaagatt acaagttgct aaaatgatta agcaaggtta tacctatgca acgattgaac
     2281 aagaatctgg agcatcgact gcaacgattt ctagagtgaa gcgttcatta caatggggta
     2341 atgatgctta tacaatgatt ttagatcgta tgaatattga aacaaatgaa taataaatga
     2401 acgataaaca atggttatct atctgcacta ataaggtaga taatcattgt tttttcacga
     2461 aaaaatttac agagtaaaag aacttaaatt tcatattaag tctttagaac tcgacactta
     2521 aaaatgctat aatcatatgt atgttaaaaa aggagtttcg gaaaatgtat gacattaaaa
     2581 aatggcgcca tatttttaaa ttagacccag ctaaacatat ttcagatgat gatttagatg
     2641 cgatttgtat gtctcaaaca gatgcaatta tgattggtgg aactgatgac gttactgaag
     2701 ataatgtcat tcatttaatg agcagagtaa gaagataccc attgccttta gtacttgaaa
     2761 tatcaaacat cgaaagtgta atgcctggtt ttgattttta ttttgtacct acagtactga
     2821 acagtacaga tgttgtattt cacaatggta cattattaga agcgcttaaa acatatggac
     2881 atagtataga ttttgaggaa gtaatatttg aagggtatgt cgtgtgcaat gctgatagca
     2941 aaatggcaaa acataccaaa gcaaatacag atttaacaac agaagattta gaagcatatg
     3001 cccaaatggt caatcatatg tatcgattac cggttatgta tatagagtat agtggcattt
     3061 atggcgacgt atcaaaggtt caagctgtct cagaacatct aacagaaacg caactttttt
     3121 atggtggcgg tatttcctca gaacaacaag cgacagagat ggcagctatt gcagatacaa
     3181 ttatcgtcgg tgatattatt tataaagata ttaaaaaagc tttaaaaaca gtaaaaataa
     3241 aggagtctag taaatgaatg cgttattaaa tcatatgaat acagagcaaa gtgaagctgt
     3301 aaagacaaca gaaggaccat tgttaattat ggcaggtgct ggttcaggga agacacgtgt
     3361 tttaacacat agaattgctt atttattaga cgaaaaagat gtctcaccat acaatgtttt
     3421 ggctattact tttacaaata aagctgcaag agaaatgaaa gaacgtgttc aaaaattagt
     3481 aggtgatcaa gcagaagtta tttggatgtc aacattccac tcaatgtgtg ttcgtatttt
     3541 acgtcgtgat gcagatcgaa ttggtataga acgcaatttt acgataattg atcctacaga
     3601 ccaaaaatct gttattaaag acgtcttaaa aaatgaaaat attgatagta aaaagtttga
     3661 acctcgtatg tttatcggtg cgatcagtaa tttgaaaaat gaacttaaaa cacctgcaga
     3721 tgctcaaaaa gaagccacag attatcactc gcaaatggta gcaacggttt atagtggata
     3781 tcaacgccaa ttgtcacgta atgaagcgtt agattttgat gaccttatta tgacaacgat
     3841 taacttattt gagcgtgtac cagaagttct agaatattat cagaacaaat tccaatatat
     3901 tcatgtagat gagtatcaag atactaataa agcacaatac acattagtta aattattagc
     3961 aagtaagttt aaaaacttat gtgttgtagg tgactcagat cagtcaattt atggttggcg
     4021 tggtgctgat attcaaaata tcttatcatt tgaaaaagac tatccagaag cgaatacaat
     4081 ctttttagag caaaattatc gttcgacgaa aacgatttta aatgcggcta acgaagtgat
     4141 taaaaataat tctgaacgta agccaaaagg actgtggact gcaaatacga atggtgagaa
     4201 aattcattac tatgaagcaa tgacggaacg tgatgaagcg gaatttgtaa tacgagaaat
     4261 tatgaagcat caacgtaatg gtaagaaata tcaagatatg gcaattttat atagaacgaa
     4321 tgcacaatca cgtgtacttg aggaaacatt catgaaatct aatatgccat acacaatggt
     4381 tggtggccaa aagttctatg accgtaaaga aatcaaagat ttattaagtt atttgcgtat
     4441 aattgccaat agtaatgatg acattagttt gcaacgtatt attaatgttc caaaaagagg
     4501 tgtaggtcct tcatctgttg aaaaagttca aaactatgca cttcaaaaca atatcagtat
     4561 gtttgatgca cttggagaag ctgattttat cggattgtca aaaaaggtga cacaagagtg
     4621 tcttaatttt tacgagttaa tccaaagctt gataaaagaa caagaatttt tagaaattca
     4681 tgaaatagtt gatgaagtat tacaaaagtc tggctatcga gaaatgcttg aacgtgaaaa
     4741 tacattagaa tctcgaagta gattagaaaa catcgatgaa tttatgtcag taccaaaaga
     4801 ctatgaggaa aataccccat tagaagaaca gtcattaatt aactttttaa cggatttatc
     4861 gttagtagct gatattgatg aggcagatac tgaaaatggc gtaacactaa tgacgatgca
     4921 ctcggctaag ggccttgaat ttccaattgt ctttataatg gggatggaag aatctttatt
     4981 cccacatatt agagcgatta agagtgaaga tgatcatgaa atgcaagaag aacgtcgtat
     5041 ttgttatgta gcaattacaa gggctgaaga ggtgttatat atcactcatg cgacatcaag
     5101 aatgttattt ggtcgccctc agtcaaatat gccatccaga tttttaaagg aaattccaga
     5161 atcactatta gaaaatcatt caagtggcaa acgacaaacg atacaaccta aggcaaaacc
     5221 ttttgctaaa cgcggattta gtcaacgaac aacgtcaacg aaaaaacaag tattgtcatc
     5281 tgattggaat gtaggtgaca aagtgatgca taaagcctgg ggagaaggca tggtgagtaa
     5341 tgtaaacgag aaaaatggct caatcgaact agatattatc tttaaatcac aagggccaaa
     5401 acgtttgtta gcgcaatttg caccaattga aaaaaaggag gattaaggga tggctgattt
     5461 atcgtctcgt gtgaacgagt tacatgattt attaaatcaa tacagttatg aatactatgt
     5521 agaggataat ccatctgtac cagatagtga atatgacaaa ttacttcatg aactgattaa
     5581 aatagaagag gagcatcctg agtataagac tgtagattct ccaacagtta gagttggcgg
     5641 tgaagcccaa gcctctttca ataaagtcaa ccatgacacg ccaatgttaa gtttagggaa
     5701 tgcatttaat gaggatgatt tgagaaaatt cgaccaacgc atacgtgaac aaattggcaa
     5761 cgttgaatat atgtgcgaat taaaaattga tggcttagca gtatcattga aatatgttga
     5821 tggatacttc gttcaaggtt taacacgtgg tgatggaaca acaggtgaag atattaccga
     5881 aaatttaaaa acaattcatg cgataccttt gaaaatgaaa gaaccattaa atgtagaagt
     5941 tcgtggtgaa gcatatatgc cgagacgttc atttttacga ttaaatgaag aaaaagaaaa
     6001 aaatgatgag cagttatttg caaatccaag aaacgctgct gcgggatcat taagacagtt
     6061 agattctaaa ttaacggcaa aacgaaagct aagcgtattt atatatagtg tcaatgattt
     6121 cactgatttc aatgcgcgtt cgcaaagtga agcattagat gagttagata aattaggttt
     6181 tacaacgaat aaaaatagag cgcgtgtaaa taatatcgat ggtgttttag agtatattga
     6241 aaaatggaca agccaaagag agtcattacc ttatgatatt gatgggattg ttattaaggt
     6301 taatgattta gatcaacagg atgagatggg attcacacaa aaatctccta gatgggccat
     6361 tgcttataaa tttccagctg aggaagtagt aactaaatta ttagatattg aattaagtat
     6421 tggacgaaca ggtgtagtca cacctactgc tattttagaa ccagtaaaag tagctggtac
     6481 aactgtatca agagcatctt tgcacaatga ggatttaatt catgacagag atattcgaat
     6541 tggtgatagt gttgtagtga aaaaagcagg tgacatcata cctgaagttg tacgtagtat
     6601 tccagaacgt agacctgagg atgctgtcac atatcatatg ccaacccatt gtccaagttg
     6661 tggacatgaa ttagtacgta ttgaaggcga agtagcactt cgttgcatta atccaaaatg
     6721 ccaagcacaa cttgttgaag gattgattca ctttgtatca agacaagcca tgaatattga
     6781 tggtttaggc actaaaatta ttcaacagct ttatcaaagc gaattaatta aagatgttgc
     6841 tgatattttc tatttaacag aagaagattt attaccttta gacagaatgg ggcagaaaaa
     6901 agttgataat ttattagctg ccattcaaca agctaaggac aactctttag aaaatttatt
     6961 atttggtcta ggtattaggc atttaggtgt taaagcgagc caagtgttag cagaaaaata
     7021 tgaaacgata gatcgattac taacggtaac tgaagcggaa ttagtagaaa ttcatgatat
     7081 aggtgataaa gtagcacaat ctgtagttac ttatttagaa aatgaagata ttcgtgcttt
     7141 aattcaaaaa ttaaaagata aacatgttaa tatgatttat aaaggtatca aaacatcaga
     7201 tattgaagga catcctgaat ttagtggtaa aacgatagta ctgactggta agctacatca
     7261 aatgacacgc aatgaagcat ctaaatggct tgcatcacaa ggtgctaaag ttacaagtag
     7321 cgttactaaa aatacagatg tcgttattgc tggtgaagat gcaggttcaa aattaacaaa
     7381 agcacaaagt ttaggtattg aaatttggac agagcaacaa tttgtagata agcaaaatga
     7441 attaaatagt tagaggggta tgtcgatgaa gcgtacatta gtattattga ttacagctat
     7501 ctttatactc gctgcttgtg gtaaccataa ggatgaccag gctggaaaag ataatcaaaa
     7561 acataacaat agttcaaatc aagtaaaaga aattgcaacg gataaaaatg tacaaggtga
     7621 taactatcgt acattgttac catttaaaga aagccaggca agaggacttt tacaagataa
     7681 catggcaaat agttataatg gcggcgactt tgaagatggt ttattgaact taagtaaaga
     7741 agtatttcca acagataaat atttgtatca agatggtcaa tttttggaca agaaaacaat
     7801 taatgcctat ttaaatccta agtatacaaa acgtgaaatc gataaaatgt ctgaaaaaga
     7861 taaaaaagac aagaaagcga atgaaaattt aggacttaat ccatcacacg aaggtgaaac
     7921 agatcctgaa aagattgcag aaaaatcacc agcctattta tctaacattt tagagcaaga
     7981 tttttatggt ggtggagata caaaaggtaa gaatattaaa ggtatgacga ttggtttagc
     8041 tatgaatagt gtttattact ataaaaaaga aaaagatgga tcgactttta gtaaaaaact
     8101 agatgatagc gaagttaaaa agcaaggtaa acaaatggct agtgagatat tatcaaggtt
     8161 acgtgaaaat gatgatttaa aagatatacc aattcatttt gcaatttata agcaatcaag
     8221 tgaagattca atcacaccag gtgaatttat cactcaagcg actgcagaaa agagtcaaac
     8281 aaagcttaat gaatggcata atatcaatga aaaatcagct ttattacctt cttcaacagc
     8341 agcagattat gatgaaaatt taaataataa tttcaagcaa tttaatgata atttgcaatc
     8401 atatttttct aatttcacac aagcagtagg gaaagttaaa tttgttgata aaaagccaca
     8461 acgattagta gtagatttac caatcgatta ctatggacaa gctgaaacaa ttggtattac
     8521 acagtacgtt actgaacaag cgaataaata tttcgataaa atcgataact atgaaattcg
     8581 tattaaagat ggtaaccaac cacgtgcttt aattagtaag acaaaagatg acaaagaacc
     8641 gcaagttcat atttacagta attaattgta aattaaagat aataaaaagg taccaatgtt
     8701 agtcataatt ttgattaaca ttagtacctt tttaatttgt taagacttat gaattatttt
     8761 tctctaacga tgtcacgaac tttgtttaag tcagtttcaa caaatgcacc aggtttttta
     8821 gtaagtttac ttacaacata tgtaacgatt acacttacaa taaatccagg aataatttca
     8881 tataagccga atatttcgtt gatatgtgcc aatggtttaa tccacgcaat ccaaacgata
     8941 acgactaagg cacctgaaac cattccactt acagcaccgg cacgtgtcaa acctttccag
     9001 taaagtgcaa atagcacaag tggactgaac gatgcaccaa atccggccca agcgttacct
     9061 actaagttta gaattgtgtc gtttggattc catgcaatcg cgatggcaac aattgctaca
     9121 actaatacag ataatcttcc aatcataaca aattcttttt ggtgcgtttt agctttttct
     9181 tcaccacgaa ttaatttata aaagtcttcc gttagtgaac tagatgttac aagtaattgt
     9241 gaagaaatcg tgctcataat tgcagctaga atcgcagcaa gtaagaaacc acctacaaga
     9301 ggatggaaga gtacttgact catcacgatg aataatgtct caggatcttc tagtttaata
     9361 tgataagcag gtacgaatgc aatacctgtt aaaccaacag ccacagcgcc taataaacca
     9421 acagccatcc agctaatacc taaacgtcta gctttaggta gcatcttgtg tgatttaata
     9481 gacataaacc ttataatgat atgaggttga ccgaaataac ctaatcccca tgaaaataga
     9541 gagataattc ctataaatga taacccttta aataaattta aatttgtagg tttcatagct
     9601 gctacatcat gaaacgttcc ccagccgttt aaattcatca tagcaacaat agggaccata
     9661 accatcgcaa ttaacataat gacaccttgg aagaaatctg taattgatac agctaaatat
     9721 ccaccaaaga aagtatagaa aatgacaatg aaagcaacta atattaaacc gaaatgataa
     9781 tctaatccaa aagcactttc aaatagttta ccaccagata cgaaaccaga atgtgtatat
     9841 aatgtaaaga atactacgat aatcaatcca gaaataatct ttaacacatt atttttatcg
     9901 tttaaacgat ttttaaagaa atctggtaat gtaattgcat ctccagctaa ttcggtataa
     9961 acacgaagtc taggagcaac aacaaagtaa tttatataag cacctaatgt taaaccgatt
    10021 gtaatccaca tagctgatag accagtgcta tagacagaac caggtagccc cataatcatc
    10081 catccactca tatctgaagc tccagctgat aatgcagtaa tatacggtcc aatactacgt
    10141 ccacctaaca tgtactcgct taggttacca gttgcttgct tgtaaccgta aaagccaata
    10201 acgattagta tcaagaagta gacggcaatc ataatataag tttgccaatt ggcatctact
    10261 tgttgactta atgctgtccc cattgtaagc atgataaaca ctccctttat ttaatttata
    10321 atttagttac ttgaaaacat tatacataaa gataaatgtt tagaaaagta ggaaaattaa
    10381 atttattctt tttgaaatac ttgcatacca atatagaaag cgctttcgta ttttttggat
    10441 atgagcaaat ttttataatt aaaattgcat aatcataata ttgaattggg ttgtctaata
    10501 ttaaaagagt gttgttttca taaaaaatga tttgataaac gaatgataat gtttatacag
    10561 tttatatgaa attaaagtag cacctcataa atacttagat ttttaattgg aaatttgata
    10621 caatttagtg atgaatgact taaaggaggc ttttattaat gacaaaagta acacgtgaag
    10681 aagttgagca tatcgcgaat cttgcaagac ttcaaatttc tcctgaagaa acggaagaaa
    10741 tggccaacac attagaaagc attttagatt ttgcaaaaca aaatgatagc gctgatacag
    10801 aaggcgttga acctacatat cacgttttag atttacaaaa cgttttacgt gaagataaag
    10861 caattaaagg tattccacaa gaattagctt tgaaaaatgc caaagaaaca gaagatggac
    10921 aatttaaagt gcctacaatc atgaatgagg aggacgcgta agatgagcat tcgctacgaa
    10981 tcggttgaga atttattaac tttaataaaa gacaaaaaaa tcaaaccatc tgatgttgtt
    11041 aaagatatat atgatgcaat tgaagagact gatccaacaa ttaagtcttt tctagcgctg
    11101 gataaagaaa atgcaatcaa aaaagcgcaa gaattggatg aattacaagc aaaagatcaa
    11161 atggatggca aattatttgg tattccaatg ggtataaaag ataacattat tacaaacgga
    11221 ttagaaacaa catgtgcaag taaaatgtta gaaggttttg tgccaattta cgaatctact
    11281 gtaatggaaa aactacataa tgaaaatgcc gttttaatcg gtaaattaaa tatggatgag
    11341 tttgcaatgg gtggttcaac agaaacatct tatttcaaaa aaacagttaa cccatttgac
    11401 cataaagcag tgccaggtgg ttcatcaggt ggatctgcag cagcagttgc agctggctta
    11461 gtaccattta gcttaggttc agacacaggt ggttcaatta gacaaccggc tgcatattgt
    11521 ggcgttgtcg gtatgaaacc aacatacggt cgtgtatctc gatttggatt agttgctttt
    11581 gcatcttcat tagaccaaat tggtccattg actcgaaatg taaaagataa tgcaatcgta
    11641 ttagaagcta tttctggtgc agatgttaat gactctacaa gtgcaccagt tgatgatgta
    11701 gactttacat ctgaaattgg taaagatatt aaaggattaa aagttgcatt acctaaagaa
    11761 tacttaggtg aaggtgtagc tgatgacgta aaagaagcag ttcaaaacgc tgtagaaact
    11821 ttaaaatctt taggtgctgt cgttgaggaa gtatcattgc caaatactaa atttggtatt
    11881 ccatcatatt acgtgattgc atcatcagaa gcttcgtcaa acctttctcg ttttgacgga
    11941 attcgttatg gttatcattc taaagaagct cattcattag aagaattata taaaatgtca
    12001 agatctgaag gtttcggtaa agaagtaaaa cgtcgtattt tcttaggtac atttgcatta
    12061 agttcaggtt actatgatgc ttactataaa aaatctcaaa aagttagaac attgattaaa
    12121 aatgactttg ataaagtatt cgaaaattat gatgtagtag ttggtccaac agcgcctaca
    12181 actgcgttta atttaggtga agaaattgat gatccattaa caatgtatgc caatgattta
    12241 ttaacaacac cagtaaactt agctggatta cctggtattt ctgttccttg tggacaatca
    12301 aatggccgac caatcggttt acagttcatt ggtaaaccat tcgatgaaaa aacgttatat
    12361 cgtgtcgctt atcaatatga aacacaatac aatttacatg acgtttatga aaaattataa
    12421 ggagtggaaa tcatgcattt tgaaacagtt ataggacttg aagttcacgt agagttaaaa
    12481 acggactcaa aaatgttttc tccatcacca gcgcattttg gagcagaacc taactcaaat
    12541 acaaatgtta tcgacttagc atatccaggt gtcttaccag ttgttaataa gcgtgcagta
    12601 gactgggcaa tgcgtgctgc aatggcacta aatatggaaa tcgcaacaga atctaagttt
    12661 gaccgtaaga actatttcta tccagataat ccaaaagcat atcaaatttc tcaatttgat
    12721 caaccaattg gtgaaaatgg atatatcgat atcgaagtcg acggtgaaac aaaacgaatc
    12781 ggtattactc gtcttcacat ggaagaagat gctggtaagt caacacataa aggtgagtat
    12841 tcattagttg acttgaaccg tcaaggtaca ccgctaattg aaatcgtatc tgaaccagat
    12901 attcgttcac ctaaagaagc atatgcatat ttagaaaaat tgcgttcaat tattcaatac
    12961 actggtgtat cagacgttaa gatggaagag ggatctttac gttgtgatgc taacatctct
    13021 ttacgtccat atggtcaaga aaaatttggt actaaagccg aattgaaaaa cttaaactca
    13081 tttaactatg tacgtaaagg tttagaatat gaagaaaaac gccaagaaga agaattgtta
    13141 aatggtggag aaatcggaca agaaacacgt cgatttgatg aatctacagg taaaacaatt
    13201 ttaatgcgtg ttaaagaagg ttctgatgat taccgttact tcccagagcc tgacattgta
    13261 cctttatata ttgatgatgc ttggaaagag cgtgttcgtc agacaattcc tgaattacca
    13321 gatgaacgta aagctaagta tgtaaatgaa ttaggtttac ctgcatacga tgcacacgta
    13381 ttaacattga ctaaagaaat gtcagatttc tttgaatcaa caattgaaca cggtgcagat
    13441 gttaaattaa catctaactg gttaatgggt ggcgtaaacg aatatttaaa taaaaatcaa
    13501 gtagaattat tagatactaa attaacacca gaaaatttag caggtatgat taaacttatc
    13561 gaagacggaa caatgagcag taaaattgcg aagaaagtct tcccagagtt agcagctaaa
    13621 ggtggtaatg ctaaacagat tatggaagat aatggcttag ttcaaatttc tgatgaagca
    13681 acacttctaa aatttgtaaa tgaagcatta gacaataacg aacaatcagt tgaagattac
    13741 aaaaatggta aaggcaaagc tatgggcttc ttagttggtc aaattatgaa agcgtctaaa
    13801 ggtcaagcta atccacaatt agtaaatcaa ctattaaaac aagaattaga taaaagataa
    13861 tttaaatcat caaactatga agatttaaaa aataaaccct tgattgctga cttagatgca
    13921 atcgagggtt tatttatatc tatagaagtc aaattactat taactttatt cattgtacat
    13981 gttaatggta aaaatattaa ttttattaat gcgttagctt taattatatt aaggcaaact
    14041 gtataataaa aaggtataaa acatttttgt ataaagacaa cattatattt acaacatcat
    14101 tttaaaggta aaatagcata actgacgaag tctatataat gaagaacggc aaaaaatgct
    14161 gaataaataa caagctttgt acatattgag atagtatttg tttaagatac aagttggtct
    14221 ttaacgatat taagaatgat gaaataagac tgagcctggg tcataaattc aatgtcctag
    14281 gcactacaat gttatattgg ctgtagttga ctgaaagaaa atacgcttgt aacaagcttt
    14341 tttcaattct agtggggccc caacatagaa gctgactttc tgtcagctta caataatgtg
    14401 caagttggcg gggcccccaa acaaagaagc tgactttctg tcagcttaca ataatgtgca
    14461 agttggggtg gggccccaac atagagaatt tcgaaaggaa attctacaga caatgcaagt
    14521 tggggtagaa cgaaataaat tttgttaaat attatttctg tcccactccc tattagacga
    14581 aacaaagatg aagttaaaat atatgaattt taagtagagg gataagatat gaggaaacgt
    14641 gctagaatca tttataaccc gacatcaggt aaagagctat ttaaaagaga attacctgat
    14701 gccttaataa aattagaaaa agcgggatat gaaacgagtg catatgcaac cgagaaaata
    14761 ggtgatgcca cacttgaagc agaaagagct atgcatgaaa attatgatgt attaatcgct
    14821 gcaggtggtg atggaacatt aaatgaagta gttaatggta tcgcagaaaa gcctaatcgt
    14881 cctaagctag gtgtcattcc tatgggtact gttaatgact ttggacgtgc attgcatata
    14941 cctaatgaca tcatgggggc acttgatgtc atcattgaag gtcattctac taaagtagat
    15001 attggtaaaa tgaataatcg atactttatt aatttagctg caggcggaca attgacgcaa
    15061 gtctcttatg aaacaccgag taaattgaaa tctattgttg gtccatttgc ttattacatc
    15121 aaaggtttcg aaatgttacc tcaaatgaaa gctgtagatt taagaattga atatgatggt
    15181 aatgttttcc aaggagaagc attattattc tttttaggtt taacaaattc aatggcagga
    15241 tttgaaaaat tagtgccgga cgctaagtta gatgacggct attttacgtt aattatagtt
    15301 gaaaaatcaa accttgcaga acttggtcat attatgactt tagcttcaag aggagaacat
    15361 actaagcatc ctaaagttat ttatgaaaaa gcaaaggcaa ttaatatttc atcattcaca
    15421 gacttacaac taaatgtaga tggagaatat ggtggtaaat taccagcgaa tttcttaaat
    15481 ctagaacgtc atatagatgt ctttgcacct aatgatattg taaatgaaga gttaataaat
    15541 aatgatcatg ttgatgacaa tttaatcgaa gaataaacag aaatagtgaa atatataatg
    15601 gcaggttagg aagcgcataa ttttgttcct aacctgttca ttttggaggt aaaattttgc
    15661 aagcaatagc taaaaatgac ataaaaacgg gaacagttgt tgatttaacg catgaaggcc
    15721 atggtgtcgt aaaaatagat cgctttccta ttttcatacc acaagcttta attaatgaac
    15781 aaattgaata taaaattatc aaagtaaaaa agaactttgc gataggtaag ttgcttaata
    15841 taaatacgag aagtgagaat cgtgttgcac cgccatgtat atattatgaa cgttgtggtg
    15901 ggtgccaact tcaacactta tcatacgaag cacaacttga aatgaaaaag gaacaagtga
    15961 ttaatttgtt tcagcgaaaa gcgcattttg ataattctaa gattaacgat acagttggca
    16021 tgacagatcc ttggagatat cgaaacaagt cccagattcc agttggtaag aatgaacaga
    16081 atgaagttat tatgggcttt tatcgtcaaa gaagccatga cattattgac atggaaagtt
    16141 gtttaataca agattcacaa catcaagaag tgatgaatga agttaaatct attttaaaag
    16201 atttaaatgt aagtatttat caagaacaat tgaaaaaagg tttaatgaga catttggttg
    16261 taagaacagg atatcataca gacgaaatga tgattatttt tgtaactaat ggtaaaaagt
    16321 ggccacaaaa aaatgctgtt gttgagaaga tattagatgc atttcctaat gtgacaagca
    16381 ttaaacaaaa cattaatgat agccactcta atgttattat gggacgtcaa tctataacgc
    16441 tatatggtaa agatacaatt attgatcaat taacagatag tacatttaaa ataagtgatc
    16501 agtcattcta ccaaattaat tctgaacaaa cagagaaatt atataataaa gcaattgagt
    16561 atgcgcaatt gacaggtaat gaagttgtat tagataccta ttgtggtatt ggaacaatag
    16621 ggctatatat ggcaccactt gcaaaacatg tatacggtgt tgaagtagta ccttctgcaa
    16681 ttgaagacgc acaacaaaat gcgactataa accaatgcaa taatacaact tttgtctgtg
    16741 ggaaagctga agaagttatt ttacaatgga aagcacaagg gattaaacca gatgttgtaa
    16801 tggttgatcc gccaagaaag ggctgtgacg aaacgtttat tcagactttg cttacattag
    16861 aacctaagcg tattgtctat atttcatgta atcccgcaac acagcaacgt gatgcattat
    16921 tattagctga aaaatatcaa cttgaagagg taacaccggt agatatgttt ccacaaacaa
    16981 cacatgttga gacagtggca ttattcaatt tgaaatagcg cacatgattt gagataaaat
    17041 ttcgccttaa taagtgaaga aagaagtcgt atattaaaat tttattacag ccaatttact
    17101 ttcttgatat gaacttttta aattaaatgt gacattgtat actatattta aagaagaata
    17161 agaatgtcat gatcgggagg gttggtaatg cataaaattg atttaacgac aaataatttt
    17221 caaatgcgaa ggtttattat cttgcaactt gttattgcgt tattcgtgat tttatttacg
    17281 tataagtggg cgttaggtgt cacagcggtt gtcgatcaaa atatcatcat caatcttgta
    17341 tatgggtttg ctggattcat tattttattg attttacatg aacttatcca tagagcatta
    17401 tttcttttat ttaaaaaaga tagcaaacct atgtttaata taaaaaaaga cagaatctta
    17461 ttccaaacag cagatgcttg cttcaacaaa tggcaattct caattattat gctgtcacca
    17521 ctaatcctat taagtactgg cttgcttatt ttaattaagg tatttggtta ttcatcactt
    17581 atttttatgt ttagtatgca taccgcttat tgctttatag atattctgct tgttgcatta
    17641 acaatatcta gtagttttaa atatgttcaa caagatgaag atagtattta tttgtatcat
    17701 caaaagccga ctcaataata aattttaata tgatgatacc aacaaaatga atttgcttga
    17761 attatttcag attacctatg ttttcttact caatcgtctt catcatcatg cttaaatagc
    17821 aattgatttt ataacaactt gtagttatct caatatagtt atcgctaagt gcaagattta
    17881 tgtaaaaaaa tcgtacacag cgtataatta tgttgagata tttttgtgcg ttataaaagt
    17941 aaaaattagt agggaggtga gcacttgact gagagacgaa ttattcatat agatatggac
    18001 tatttttttg cacaagtcga aatgagagat aaccccaaat tgaaagggaa accagtcatt
    18061 gttggtggta aagcaagcag taggggtgtt gtctctacgg catcctatga agcacgaaaa
    18121 tacggcgtcc attcagcaat gcctatgtca caagcgcata aattatgtcc aaatggatac
    18181 tttgtcacaa gtaattttgg tgcttaccga gaaacatctg cgcagattat gtctattttc
    18241 cgaagttata cagataaggt agaaccgatg tcattggatg aagcgtattt agatattaca
    18301 gaattagtga gaccagacct tcctgcttcg aaaattgctc agtatattag aaaagatatt
    18361 ctagagcaga cacatttaac agcatctgca ggtgtttctt ataacaaatt tttagctaaa
    18421 ttagcgagtg gtatgaataa acctgatggt atgactgtga ttgattatca aaatgtccat
    18481 gatattttga tgacattgga tattggagat tttccaggcg taggtaaagc ttccaaaaaa
    18541 gtaatgcatg ataatggtat ttttaacggt agagatttat atgagaaaac ggaatttgaa
    18601 ttaatacgtt tgtttggaaa aagaggtcgg ggtttatata acaaggcacg cggtattgac
    18661 catagtgaag tgaaatcatc aagagtaaga aaatcagtag ggactgaacg cacatttgca
    18721 acagacgtga atgatgatga agagatttta agaaaagtat gggaattgtc aggtaaaaca
    18781 gctgaacgtc taaataaatt acagaagtca gctaaaactg taacggttaa aattaaaact
    18841 tatcaatttg aaacgctatc taaacagatg agtttaagag attcggttag ttctgaagaa
    18901 gatatttata atattgcata tttactttat aacgatttaa aagaccctga tgtaccaatt
    18961 cgacttattg gtgtcactgt aggtaattta gaacaatcaa cttataaaaa tatgacgata
    19021 tatgacttta tataaaataa agctccctgc aaagtttaca tttttacaat gcttactttt
    19081 gaagggagta ttttatttag tcctagcctt tatcttttag atttttacca taaatattag
    19141 ttacatatgt taaattttca taatttttca gtagcctaaa agtaatcatt gcgcatgctt
    19201 tggcatcatt caatgcatca tgatgaccat gaaaatctaa ttgataaaac tccatcatat
    19261 gttttaaacc gtatcgatac gaatcaacgg ttcttttagc aagttgataa ctacaaaagt
    19321 aagttaaatt tggagttggt aaaccaatat tttgaatgct ttgatgtaag acgttcatat
    19381 caaatgccgc gttatgtgcg acaacaggta aatctgcaat aaattgcatc atatatggaa
    19441 atacgtagtc aaacgtaggt gcattttcaa catcttctgg ttgtatgcca tgaattttaa
    19501 tattttgttg tgaaaaatag tcttgcggat tcacaagagt atgaaatgtt tctgttattt
    19561 gactatcaat gactttaacc attccgacag aacaaatact tgtacgttta ccatttgctg
    19621 tttcaaaatc aagtgcaaca aacgcatctt gtatcatgta gtcaatcctt tcaattaatt
    19681 aaatgtatat attaatagac ttttagtcat ttgcttacaa acgatacata atattatacg
    19741 tgaaggtata acgtgaaatc tatcatttgg atgtaactct gattaaaaag taaatgatga
    19801 tatgctttaa aatcgtcaaa ttaattcaac atcaaattta gagttattaa cattagtagt
    19861 ttaaaaaata aatgactaaa aatacaataa ttagccttga aaatgaaaaa acgcagatca
    19921 atgattcaga aaatgaatct agtgatctcc gtaaagcaaa ataatataga cattgggtta
    19981 ttgtaagtga cccaaatgcc tatcatgtcg tatgatattt atctgtctat tgtagtgatg
    20041 tttaattatt cttcgtcgaa tgtacgagcg ccaagttctt tttcgtaaag atataatgca
    20101 ttgctgtcat cgccgatacg agttaaataa ttgatgtgag tttcaaacat tgattcttct
    20161 tcgacttgtt catctaagaa ccagtttaag aatgagatag ttgcataatc tttatcttga
    20221 cgagcgattt cagataagtt atagaaacgt cttgttactt cttgttcttg agataagctg
    20281 tctttgaaag tttctagtat gcttgaaaag tcaatttttg gtgctgaaac tgctctgaat
    20341 tctgcatgtg cacctctgtc gttaatatag ttataaatct tttgtccatg gaaacgttct
    20401 tctttagctt gttgaatgaa gaagtttgca aatccttcgt acgattcttt atcacagtat
    20461 gctgccattg ccatatatgc gtgtgctgca aagtactcat ggttcatttg atcatttaat
    20521 gcttctaata aatttttact taacattttg atacacctct tatttgtaaa ttaaaattac
    20581 tatgaattaa acttagtata acaaactcat tctcaattgt aaataataat aattataatt
    20641 taatgcctta
//
LOCUS       DAKTIP010000027        19867 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAKTIP010000027 DAKTIP010000000
VERSION     DAKTIP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19867
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.027"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(178..768)
                     /locus_tag="O0305_000773"
     CDS             complement(178..768)
                     /locus_tag="O0305_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCY0273929.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
     gene            complement(922..2184)
                     /gene="clpX"
                     /locus_tag="O0305_000774"
     CDS             complement(922..2184)
                     /gene="clpX"
                     /locus_tag="O0305_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCY0273930.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(2335..3636)
                     /locus_tag="O0305_000775"
     CDS             complement(2335..3636)
                     /locus_tag="O0305_000775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCY0273931.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(3684..3794)
                     /locus_tag="O0305_000776"
     CDS             complement(3684..3794)
                     /locus_tag="O0305_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273932.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(3799..4728)
                     /locus_tag="O0305_000777"
     CDS             complement(3799..4728)
                     /locus_tag="O0305_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273933.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLADFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLK
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(4747..5355)
                     /locus_tag="O0305_000778"
     CDS             complement(4747..5355)
                     /locus_tag="O0305_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCY0273934.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(5497..5853)
                     /gene="rplT"
                     /locus_tag="O0305_000779"
     CDS             complement(5497..5853)
                     /gene="rplT"
                     /locus_tag="O0305_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCY0273935.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(5900..6100)
                     /gene="rpmI"
                     /locus_tag="O0305_000780"
     CDS             complement(5900..6100)
                     /gene="rpmI"
                     /locus_tag="O0305_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCY0273936.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(6129..6656)
                     /gene="infC"
                     /locus_tag="O0305_000781"
     CDS             complement(6129..6656)
                     /gene="infC"
                     /locus_tag="O0305_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCY0273937.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(6685..6810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(6885..8378)
                     /locus_tag="O0305_000782"
     CDS             complement(6885..8378)
                     /locus_tag="O0305_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0273938.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(8804..10741)
                     /gene="thrS"
                     /locus_tag="O0305_000783"
     CDS             complement(8804..10741)
                     /gene="thrS"
                     /locus_tag="O0305_000783"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCY0273939.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(10939..11061)
                     /locus_tag="O0305_000784"
     CDS             complement(10939..11061)
                     /locus_tag="O0305_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273940.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(11154..12074)
                     /gene="dnaI"
                     /locus_tag="O0305_000785"
     CDS             complement(11154..12074)
                     /gene="dnaI"
                     /locus_tag="O0305_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCY0273941.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(12074..13474)
                     /locus_tag="O0305_000786"
     CDS             complement(12074..13474)
                     /locus_tag="O0305_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCY0273942.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(13475..13945)
                     /gene="nrdR"
                     /locus_tag="O0305_000787"
     CDS             complement(13475..13945)
                     /gene="nrdR"
                     /locus_tag="O0305_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCY0273943.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(14155..15180)
                     /gene="gap"
                     /locus_tag="O0305_000788"
     CDS             complement(14155..15180)
                     /gene="gap"
                     /locus_tag="O0305_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0273944.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(15350..15973)
                     /locus_tag="O0305_000789"
     CDS             complement(15350..15973)
                     /locus_tag="O0305_000789"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCY0273945.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(15989..16861)
                     /gene="mutM"
                     /locus_tag="O0305_000790"
     CDS             complement(15989..16861)
                     /gene="mutM"
                     /locus_tag="O0305_000790"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCY0273946.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(16877..19507)
                     /gene="polA"
                     /locus_tag="O0305_000791"
     CDS             complement(16877..19507)
                     /gene="polA"
                     /locus_tag="O0305_000791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCY0273947.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSCNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
BASE COUNT         6007 a         3808 c         2680 g         7372 t
ORIGIN      
        1 aacataattt aaaatccaat taagtacgat gcttaaataa attataatct ttctaattta
       61 cttttgagtt tttgacaaaa tgatgaacgt tgaaaaatgt tatcaattgt gtactaatta
      121 aaagtttcgt gcttacatta tttttaaaat ttttgtataa gttttgacgt acaaaaacta
      181 tgaaatatac ggttcaatta aattccatat ttgttgttgt ttattatttt gaattgatga
      241 ataacttaca attgtatcgt ctgggtccat atctaattgt gtcttaatat ttttaatatg
      301 cttttgaacc ttaccttttg gaattttgtc ttctttagtg catataacta aagtaggaat
      361 atcaaaatgt ttcaaataat tgtacattaa gatatcatct tgtgttggat catgtcttaa
      421 atcaactaat tgaataacta attgcaaatt ctctctctta gttatatatt cctcaatcat
      481 tttcccaaat ttttcacgtt gtgttttact tactttagca tatccatacc ctggaacatc
      541 cacaaaaata agttgttcat ctatattata aaaatttaac gtttgcgttt tgccgggttg
      601 ctgtgatgta cgtgccatat tttttctgcc aatcatacta ttgataaatg tagacttacc
      661 tacattagat cgaccgctca gtgcaacttc agacaattct gtttctggat attgttcttc
      721 ttttactgca ctaatgatta attctatatt attaggatta actttcattt tatatcctca
      781 cttttttata ttctctttca attctaccat tatataatat ttgaaccttt cggtatacat
      841 tataaaaaag ctccgatcaa agttaaacta tttgagcgtc aactttgatt ggagcttttc
      901 acttttataa cacatcaatg attaagctga tgttttacta ttattaatta aattgccttc
      961 tgcgtcgtat agttctggtt cagtttcttc attaattgtt tgtgctgtaa taactacctt
     1021 cgttacattt tcgttagaag gcacatcaaa cataatatcg attaacgatt cttctatgat
     1081 tgaacgtaaa ccacgcgcac ctgtttttct ttcaattgct ttttcactaa ttgctgataa
     1141 agcttcttca gtgaactcta aatccacatc atctaattcc agcattttag tatattgttt
     1201 cacaagtgca tttttaggtt gcgttaagat gtttttcaac gcagttacat ctaatgtttc
     1261 taaattagct acaattggca cacgtccgat aaattcagga atcaaaccat aggcttgcaa
     1321 atcttctggg cgaatttgtg ctaataatgc ttgttcgtca tatttatcag cttcattgct
     1381 tgagaaacca ataacttttt caccaagacg gcgcttaatc acttcttcaa taccatcaaa
     1441 ggcaccacca agaataaata agatatttgt tgtatcaatt tgaatcattt cttggtttgg
     1501 atgtttgcgt ccaccttgtg gcggaacact tgcagtcgta ccttctaaga ttttaagcaa
     1561 tgcttgttga acaccttcac ctgaaacgtc acgtgttata gatgtgtttt cagatttacg
     1621 tgcaatttta tcaatttcat ctacataaat aatacctttt tcggctttat caatgtcaaa
     1681 gtcagctgct tgaattaatc tcaacaagat attttcaaca tcatcgccta cataaccagc
     1741 ttcagttaaa cttgtcgcat ctgcaattgc aaatggtaca ttcaacgtct tggctaaggt
     1801 ttgagctaat aatgttttac cactacctgt tggcccaatt aatgcaatgt tacttttttg
     1861 taattcaaca tcatcttctt ttggtcctaa ttgttgaata cgcttatagt ggttataaac
     1921 agctacagct aaagattttt tagctttttc ttgaccaata acatattcgt ttaaatgatc
     1981 cataatttct ttaggagtag gtaattctgt catcgcttca gaagtgtttt gagctaattc
     2041 ttcttcgacg atttctgagc ataattcaat acactcatta caaatatata caccacttcc
     2101 tgctacaagt ttttttactt gatcttggtc ttttccgcag aaagagcatt tcaaattttc
     2161 ttcatcttca ttgaatttaa acattctttt tacaccccta ttcgttaaag actatactag
     2221 attggatgtt acaatgcaac atattaacat acaaactttt tgcttaaaga atagtagcag
     2281 atacataagc tatgcatctg ctactcattt ttgtaattta atatttaatg aagattaatc
     2341 ttctttagtt ccttcaacga actttgcgtt atctcttaat aaatcgataa ctttttggat
     2401 acgaacatca tttttaatga tatcagtatt acctaaagta tttttgatat cttcaactga
     2461 gatattaaat tgtttactca ttttttctaa ttctttatcg atatcttcat cagtagcttc
     2521 gattttttca gcttcagcga tcgcagttaa agttaagtta gttttaacac gttgttctgc
     2581 atcgtctttc atttgctctc ttaattgagt ttcatcttga cctgagattt ggaagtacgt
     2641 ttgtaaatct aaaccttgtt gttgaattct ttgtgcaaat tcagacacca tacgatctaa
     2701 ttcagtatta accattgctt caggaatatc gattgttgta ttatcagtag cttttgtaat
     2761 cgcttcttct ttttcaacat tttcagcatc tgtagctttt tgttcagcta aacgtttacg
     2821 taagttttct ttgtactcgt ctactgtatt tgcttctgca tctaattcat tagcaatttc
     2881 atctgttaat tctgggactt ctttaaattt aatttcgtta acttttgttt tgaaagttgc
     2941 ttctttaccg gctaattctt cagcatggta ttcttctggg aatgttacga caacatcttt
     3001 ttcttcgtca actttcatac cttctaattg ctcttcgaaa ccaggtatga atgaacctga
     3061 accgatttct aaatcgtaac cttcagcttg tccaccttcg aattcttctc cgtcaactga
     3121 accactaaag tcgatgttaa ctgtgtcgcc attttcaaca acaccatctt ctttaacgac
     3181 catttcagct aaatgtccta agctgtggtc aatcgcttct tgtaactcat catcagataa
     3241 ttcagtttct tgtttttcaa tttcaagacc tttatagtct cctaatttaa cttctggctc
     3301 aactgtaact gttgcttcaa aaatgaaatc tttacctttt tcaatttgag taacacttac
     3361 ttctggttgt gcaactggtt taatatcagt ttcgtcaatt gcttcaccat aagcatctgg
     3421 taataaaatg tcgatagcat cttgatataa tgcttctaca ccaaagcgtt gttcaaaaat
     3481 tggacgtggc actttacctt tacggaatcc aggtacgtta atttgtttaa ccactttttt
     3541 gaatgcttga tctaacgctt tgtttacttt ttctgcagga acagtaacag ttaataaacc
     3601 ttcgttacct tccttttttt cccaagttgc tgtcatgtat atatacctcc atgattaact
     3661 aatttatttt ttcaacttcc ctattatatc ataggtctat tccctataca aacattgaaa
     3721 tcacaacgtt tatatatttg taaatcaact tttttcgtca aaactatatt aaaatgctga
     3781 gtattatttt gcaacaattt aactattatt attcaaatct aattgctgaa taaaattaaa
     3841 tgcatcgata ctttgtaatt cacatttaag tcctaacata tttttgaaat aacattcgta
     3901 tgcattaatc catttattcg tttcaaacaa agtttcaata tcaattggat aaatcatgat
     3961 agagtgatta ttcataatat ggtgtgcttc ttcagcgata tgtaaagcac catcatttaa
     4021 tgtttccata acgttaggta taactttttc tttaagtgtt gtatgttcta gcccttttaa
     4081 attagctggt acaaaagtta catccatacc atacttttca attgtcagtt cttgtgtaca
     4141 atttgcgaac cttaaatact caatcattaa actaatgagg ttataactgt acgtattaga
     4201 ttttaatata tataataccg tttcttgaaa ttgaaaatga ccattgtcta ttaacttcaa
     4261 aatcaagtgc gtctgttccc tcattgataa cgtatcaaaa tcagctaatg actgagtcaa
     4321 tcgtttttca tcttcaatta attttgactt agcaaattct ttaagaggat gtaatgccat
     4381 tcttgttttg tgatctttga cttcatcaat aatttgatga attacttcta ccgcttcaaa
     4441 atattgtccc aaaccaatca aacttttcac gtaataaatc atcaatgcat catattgttg
     4501 tatgccagtt ttcaataaga caattgtttc ttctcttaat tcaagaaatg accccgtctc
     4561 atataacata cggcatttca tcattgctat ttcttcagtg agttcaaatt gacgttcata
     4621 ttgcacaata tattcataca tcaggtcata gttttggtct tgttcagcac gttttatatc
     4681 tttatataac tttttcgaag agttaggaaa ctgaataata tctgacatat atgtcacctc
     4741 gtttgattat aaatcttgaa ggattaatgc actccacgtt tcgaaatcat catatgactc
     4801 tatgttgcct ttttcaaccc attttattcg taaagtatct gtttctttag cttctacatc
     4861 attcgtactt acagtgattt taaatacaac accaatatgt accttgccca cttcattatt
     4921 atcgtcatta ataaaaccga tatattccat attttgtgaa tcttgctcac ttaaacctac
     4981 ttcttcttct aattctctct gtgcattaac tctcaatact tcgttaatag attctgctcc
     5041 tggaacatca ttcatatgac cgcctacacc tattgaagat tgtccatgca atcgagcttc
     5101 tccaccgcca gataatcgtt catacactaa tatctcgcca tgctcatttt caagtaaaca
     5161 ataagaaatg agttgtttat atgatggatc ctcttccata tcgccgcgtc gcttaacttc
     5221 atattgactt agcgcatcaa aaatattttg accttctggt ttattcttat ttaaaaaccc
     5281 attgaaagta ttcttttcat tattaaaaat aatttctcta ggtactacaa tgatttgttc
     5341 atcaaattta gacatcgtat aaaactcctc actttaatgt ttgttctctt aaatgcattt
     5401 tagcaaatat gtcatatttt agcgattaaa tgtggcattt tgctatttaa aaaataaaaa
     5461 agagatgaca atgccatctc ttttttataa gtgttattat tttaaagcat ctttagcttt
     5521 agttactaat tgagcaaatg ctttttcgtc agaaattgcg atttctgata acattttacg
     5581 gttaatgtcg ataccagctt ttttcaaacc gttcattaaa cgtgagtagc tcatttcatg
     5641 ttgacgagct gctgcgttga tacgtgtaat ccataattta cggaagtcac gtttacgttg
     5701 acgacggtca cggaaagcat attgacctga tttcattact tgttgcttag ctactttgta
     5761 taatgtatgt tttgaaccga agtaaccttt agctaattta atcgtttttt tacgacgcgc
     5821 tcttgttact gttccacctt taactcgtgg cataaaaaat tcctccttag gtaattccta
     5881 ccgatttctg tatttgttct tattttttgt atgctaataa ttgttttaca cgtttcatat
     5941 cgctcttaga cactaatcta gctttacgta attgacgttt ttgtttagtg ctcttgtttg
     6001 cgaataagtg agatgtgaaa gctcttgaac gttttaattg acctgaagca gttcttttaa
     6061 cacgtttagc tgctccgcgg tgagttttca tttttggcat aatataagtc ctcctgtaat
     6121 agctttaatt atttttcagc tgttggcgct aacatgataa acatttgacg cccgtccatt
     6181 ttaggttttt gttcaactgt tgctatatct ttgcattcat ctgcatattt ttctagcaca
     6241 cgttgaccaa tttccttatg cgtaatggca cgccctctga aacgaataga tactttacat
     6301 ttatcgcctt tagttaagaa tttacgtccg tttttcaact tcgtttggaa atcatgttcc
     6361 tcaattgttg gacttaaacg aatttcttta acattgataa ttttttgttt ctttttcatt
     6421 tctttttctt ttttctgttg ttcgaatttg aatttaccgt aatccataat tcttgcaact
     6481 ggtggtttcg cattcggtgc aacgaccact aagtctaaat ctacacgttc agccatttct
     6541 aaagcttcac gctttgattt aacaccaatt tgttcaccat cttgaccgat taaacgtaat
     6601 tcttttgcac gaattttgtc attgatttga gtttgatctt ttgctatggt tgacacctcc
     6661 aaaattttta cgaaatttgc accaagcaaa aaggaagagc aggtataaaa tacccgctct
     6721 tccttataca cagttatgtg taatgtgatt aacctgccaa ctgctttatg cgtcgctaca
     6781 ggtgagaagc gggtgcttct acttggttcg tttcgtattc aacgttatta atcatatcaa
     6841 caattcacat ttaagtcaac actataactg taattatttt tattttaacc ttttatttca
     6901 tccattgaca cgtcttgacg taaatctact tgttctaatg gaattttttt cgttttatat
     6961 cgaagcttat gataaataaa gaatgctaaa aatactggaa ttcccatata cgtaattaag
     7021 aagcgactaa aattaaaatc tcctgtttta ataaagtcaa catcttgacc aataattact
     7081 acaatacata aaaagccagc aaataatggt ccgaatggga ataatttagc agtatatttt
     7141 aatttagatt tgtcataatt ttgtttatca aatgctcttc taaatcgata atgacttact
     7201 gctataccta cccaagcaat aaaaccagtt aaaccacttg ctgcaacgat atattcgtat
     7261 gcaccttttg taaggctttg taatacgaaa attatcacta caatgattgc tgtaacgagt
     7321 aacgacatat atggcacacc gtttttattt gttttaccaa atgcttcaaa cgctaattta
     7381 tctttactca ttgaatatag cattcgagtt gaagcataca tacctgagtt acctgctgat
     7441 aacacagacg ttaaaatgac tgcattcata aatgatgctg caaacgcaaa tccagcattt
     7501 ttaaacacta atgtgaatgg agacgttgct acattatcac taccccccat taatgcacta
     7561 ctatcataag gaattaacat accgataaca aaaatggcta aaatgtaaaa taataaaatt
     7621 ctccagaata cttgtttaat tgctttcggc acagcacgtt caggattttc tgattcacca
     7681 gccgtaatac caattaactc agtaccttgg aatgagaaac cagcgattaa gaatacacct
     7741 agaattgata acaaacttcc tcctaagttg ccaccaagaa ttggaccttc acctttatta
     7801 aatatttcga atcctacaac atgaccaccc atgattccga caatcgttaa taaaccaatt
     7861 gcaatgaaaa caataactgt aaccactttt atcaatgcca accagtattc actttcacca
     7921 tagacgcgaa ctgataacga attcagacta aaaattataa ctaagaacaa cgcactccat
     7981 gcccaagcgg gtatgccttg caatggtgtc caatattgaa tgacttgtgc tgcaatcgta
     8041 atatctgctg ctacagtcac tacccagtta aaccaatagt tccaaccaag cgcaaaccct
     8101 aaagatggat caacaaatct tgtagcatat gtactaaatg aacctgatac tggcaaatac
     8161 gtagccattt cgccaagtga cgtcattaag aaaaatacca ttattccgat aattgcgtat
     8221 cctattaatg cacccaaagc acctgcatca tgaattgctc caccagaagt tacaaataaa
     8281 cctgtaccaa tacaaccccc aatcgcaatc atagaaatat gacgatcttt aagtcccctt
     8341 ttgacaacat tgttactttc attttgaact tttgacatat aagctccctt tccatgtttt
     8401 agaggccaag aaataaataa cctttattca ctttcatacc cttttttaca gaacatagtg
     8461 atattaaaag taaaaaagtg gctacatacc tttaagatat gcaaccactt tggcaattat
     8521 ctatataagg tagcacatca cattctgtga cagtacagtt cctattcgaa aactgtccca
     8581 atagatataa tttatggttt atatctattt cggcatcttt acctttcact tgttcaactt
     8641 atgtaccata aatacttctg acaagttact aattaaacat gcaacctcta actcaattta
     8701 atattttaac taacttgtaa tatacaggat tcatcacgca taatcaaccc tgtaaaactt
     8761 gatacgcaat aaaagtttta aagcatttta ttgcgacaac tgtctatcta tgttttttca
     8821 aacgaatttc atcaactaga ttccagataa attcatcttt ttcaactgtt tcttggtctt
     8881 gcgatccata ttgacgcaca ttcacttgat tattttcaac ttccttatcc ccaactacga
     8941 tttgataagg tattttttgc atttgagctt ctctgatttt ataacccatt ttttcattac
     9001 ggtcatcaat acttacacga acgccttgag atttcaattc atcttgtaat tggcgcgcat
     9061 aatcataatg taaatcaacg ttaactggaa tgatttgaac ttgttttggc gctaaccaag
     9121 ttgggaatgc acctttagtt tcttcagtta aaaatgctac aaatcgttcc attgttgata
     9181 caacaccacg atgaataaca actggacgat gatgttcacc atcttgacca atataagtta
     9241 aatcaaaacg ttctggtaat aagaaatcaa gttgtgctgt tgataatgtc tcttctttac
     9301 ccatcgctgt tttaacttga acatctagtt tcggaccata gaatgccgct tcaccaatcg
     9361 cttcttcgta cgataagcca agctcatccg ctgcctcttt aagcatattt tcagctttat
     9421 tccacatatc atcatcatca aagtactttt ctttatcttc agggtctcta taacttaatc
     9481 taaagctata atcctcgaaa ccaaagtctt tatacacatc aataatcatg tttacaacgc
     9541 gtttgaattc ttctttaatt tgatcaggtc gaacaaagat atgtgaatca tttaaagtca
     9601 taccacgaac acgttgtaat cctgatacag caccacttgc ttcatatcta tgcatcgttc
     9661 ctagctcagc gatacggata ggtaattcac gatatgaatg tggtttattc gcataaatca
     9721 tcatatgatg tggacagttc attggacgta ataccataga ttcagtttca tctaactgca
     9781 ttggtgggaa catatcttct tgatagtgat cccagtgacc agatgttttg tataaatcaa
     9841 cattagcaag tactggtgta taaacgtggt catatcccat gctaacttct ttatcaacaa
     9901 tgtaacgttc aatttcacgt ctaattgttg caccgttagg taaccataat ggcaaaccag
     9961 caccaactaa ttggctattt gtgaatagtt ctaactcttt accaatttta cgatgatcac
    10021 gttctttacg ctcttctaac atttgtaaat gtgctttcaa ttctttttta tcaaagaaag
    10081 cagtaccgta tatacgttgt aacattttgt tgttactatc tccacgccag tatgcacctg
    10141 ctgtagataa tagtttaaac tctttaattt tagctgttga tggaacgtga actccacgac
    10201 ataaatcagt aaaatcacct tgactatata atgttacatt ttcatcttca ggaatcgcgt
    10261 cgattaattc taatttgtat tcatcattgc tgaataactc tttcgcttca tctcgtgaaa
    10321 ccacttttcg ttcgattttc atattttcgt taacgatttg tttcattgtt ttttcaattt
    10381 gttcaaagtc atcagatgag atgttttggt caatgtcgaa gtcatagtag aatccacctt
    10441 ctattacagg acctacacca aatttaacat taccatataa ccttttaatc gcgtgtgcca
    10501 ttaaatgtgc agtagaatga cgtaatacct ctaacgcttc ttcactacct ggtgtcacaa
    10561 tttcaattga tccatcagtt tcaagcggtt tagttaaatc tacaagttgc ccgttaaatt
    10621 tgccggcaac agctttttta cgtaatccag gactaattga ttgtgctata tcttcagtag
    10681 tagtaccttt atcaaacgcc tttttattac catctggaaa ttgaatatta atttgttcca
    10741 tgttaaaccc tcctatttgc tttataaata cttttatctg acctcgttaa aaaattgaac
    10801 aacaatcatc gttttaacat taaacctatt aacaataaac agggaattta tgacaccaac
    10861 aaacatttat gtctgaaaaa aacaaaaaat cccgccccta tacaagggac gagatcgtcg
    10921 tggtaccacc ctagttatct aatacaattt atgtattaaa tatctctgct taagataacg
    10981 gtcttgatcc gggtattcat tacaaatacc ataaatgaag tagtaatcat ctaatttatt
    11041 aaccatattc tcatcaaaca atggctttct gtgtaatatt gattagatca tcttgtcttc
    11101 aatcatttaa acgatttaga tttgtattca ttatacacca atcattttaa aattcaattg
    11161 tttctgaaat tttctcctga taaaaagtat ggtgttgaca aagatttgac acgttcaata
    11221 atacgtgctg ctttagtctt ctcttcacca tcacgagtca tcgctaaatg atgttccaat
    11281 tcactatagt caaaattaga actaaagaat gttggtaatt catgaaccat tcgataatgt
    11341 agcaaaggtc caattacctc atctctcacc catggagtca cttcttcagc cccaatatca
    11401 tcaagcatta aaatgtttgc ttctcttacg cgatgtaatt tcttttcaaa agaaccatct
    11461 ttaaagccac cttttaatgt tctaataaat tccggtaaat aaataattgt cgaacgtacc
    11521 ttcttagatt tgagctgatt cgcaattgca cctagaataa aagatttacc tgtcccaaat
    11581 ggaccataaa ggtaaaggcc tttcacttgt tccccattag ttattgctgt acaaatatca
    11641 tctgctgcca tagctacatc aagacggtct cgatgattca tataaatatc tttcaatttg
    11701 gcatttaaag tatctcgttg catatgatga gatgtaatta gctcagcttc aaagcgttct
    11761 tcgtcgtact tgattttaca tgggcattgt aaatagcgta ttttaattcg gttattatca
    11821 acatataact caggcacatg cccctttacg aaatttggac aatcagcaaa tttatgaccg
    11881 tcataatgtt tttgttgatc tttatactct tgtaacacat ttaagtcttc atcaatcata
    11941 gcattcgtta attcagctcg atgcgcttcc aaaaattgct taacatctgg gtcattgatt
    12001 acttcttttt ttatcttttc tattcttttt tcaaagtcct gcgacgtgtt aattatactt
    12061 ttaaattgct tcattattga ctgtcctcct cccatttttt agataattta tctagaaatg
    12121 cttgtcgatc ttgctctaat tgttgatcat ctacgctatt atctttagcc gaatcttctt
    12181 cactaggttt atctctattt tctaaccatt taggtgtttt ttcttttgaa atacgattac
    12241 gctgcccata gtatgaacca cgcttttggt aatttccgct agaaccctca tttttaggtt
    12301 gattaacttt tttagcgtaa ttatatgctt ctttagctgt cttaatacct tttttcttcc
    12361 aatttgatgc tatttccaaa atatacgctt taggaagttt catatcttct tttaacatga
    12421 caaattgcaa caaaatatta atgacgccaa aagacatttt ttcacgttca attaattctt
    12481 caaccattgt cttttgcgat atagttggtt ctgattcaga ccaagaagct aacatatcaa
    12541 ttggactcgt ttgttcaagt aactcaaacc attcatcact ttgtggcttt ggattcactt
    12601 ctgaagattt gcccgccgaa gatgatgtag caggagattt cacctgtaat ttaggcattt
    12661 gattttcgtg ttccattaag taatacgagc gtgcttgttt acgcatttct tcaaaggata
    12721 actgttgtcc acttgtaatt gaatttaaaa taacatgctt catgccatct gctgttaaac
    12781 catataaagt cgcgagttgt gtaattaaac gctttgcatc tttggtaaca atgtcttgac
    12841 taataaaatg tttacctaac atttgtctca acatttcaaa gtcaaaagat tcatttgata
    12901 aatcgatacc ttggtacggt tcattaatcg gaatatcact tgtatcgata tctatttttg
    12961 tagacggcac tttaaaaaca tcagtaaatt gtcttgttac ctgtttaaat tcactcaaat
    13021 caatttgttg atactcaaag tatttcttca actcatgaaa tcgacgatgc tcgacttcac
    13081 tatataaaaa gattgacaac attggatcat taaaaaataa atgtgctgaa ggcggttgaa
    13141 ttaattggta aacaaattgt gtttcttgtt catcatgttt gacaaacgcc tttaacaatc
    13201 caatcgcttc aagtaagtcc atttgttgtc taaactctag taaattaatt ttaagttcat
    13261 tcataaaaat ataatgagaa agaatcaatg tttcattatg actttcttta acgaattgag
    13321 tcataaaatg atataaaccc actgcttgcg ttccaattag cggtgtatac agtcgattca
    13381 atacctctaa atgattcgta tttaaatcaa agtgttgcat aactttgaat tgatcctttg
    13441 gtcttaagcc gaattcgaag gcttgtcgtc ccatttaagc atcactccgt ttgttttcgc
    13501 ttaaaatccc ttgcatcgat gctaacaatt gatcaacatc tttaaattct ttatagactg
    13561 atgcaaatct aacatatgaa acttgatcaa catgcattaa caagttcata acgtgttcac
    13621 ctatatctcg tgaagacact tccgtatgac cttcatctcg taattgccat tcaaccttgt
    13681 tagttatgtc ttcaagttgt tgatatctaa ctggtcgttt ctcacaagaa cgcacaagtc
    13741 cattaagtat cttttctctt gaaaactgct ctcttgtgcc atcttttttc acaactataa
    13801 gctgactaac ttcgatatgt tcaaatgtag tgaaacgtgt tccacaattt tcacattctc
    13861 ttcgtcttcg aatggcattt aattcatcgg catgccttga atctacaact ttagattgtg
    13921 tagaattaca tttcgggcat ttcattacat caccctcttt attttgatta tgcctaatta
    13981 tactataaat ctagagatga aaaaagaatc cctcaattta attcatttaa ccaaataatg
    14041 aaacaataaa aaacattata tcgttactta ttaagtaatt tgcatgacaa tattattgta
    14101 ttaaaaataa aaaacctaac tccgaagtca gagttaggct ataaattaat tgtattaact
    14161 tgcacttaca gtttcttttg atgtcaaaag tgctccaatt tgctcagcaa catctacaac
    14221 tctatttgaa taaccccatt cattatcata ccaagcaata acttttactt tattccctga
    14281 catgaccatt gttgattttg catcaataat agctgaattt ggattagtat taaaatcaac
    14341 agacactagt ggttgatgtt cgacttctat gataccttct aaacctgcat tttcaaaagc
    14401 ttggtttact tcttctgcag ttacttcttt ttctaaatca acaactaaat caacgagcga
    14461 tacattcttt gttggtacac gtaatgccat gccgtgtaat ttaccttcta attctggtaa
    14521 tacttctttt aaagctttcg ccgcaccagt agaagtagga ataatgcttt cattacatga
    14581 acgtgcacgt cttaaatctt tatgtggatt atcaatattt ttttggtcat ttgtaatagc
    14641 gtgaacagta gtcattaaac cattaactat tccaaactga ttatttaaaa cttttgcaac
    14701 tggaccaatg caattagtag tacatgaagc attactaaaa atgtcaaatg cttctatatc
    14761 taattggtta tcatttacgc ctttaactac catttgaaca tgtccacctt ttgaaggacc
    14821 agttaacaaa acttttttgg cacctgcttt aatatgtgcg atggctttat caccatgatt
    14881 aaatttacca gttgcatcta tagcaatatc gatatctaat tctttccatg gcaagttttc
    14941 aggattgcga tcagcaacca atttaatttt atgatctcca acttgcaatc cattttcaat
    15001 cggttcaact tttagattat attttccatg tgtcgtatcg taattgatta aatgtgcaat
    15061 tgtttcgggt ggataactag catttatcgc tactacattt aaatttttat tttgtaatgc
    15121 aatacgtaat accattcttc caattctacc cataccatta attgcaatat tcgttgacat
    15181 taaaagcacc ccttgatctt tatgtgttat actttatgga attagtatag catattatat
    15241 gaaatttgaa agcgttttcc gttgatttat ttatcttttt tataaaaaat agtcattact
    15301 tacatacatc aacgcgtacg taaagtaatg acttatttat agtgtaatat taatcttctt
    15361 ctccgtaatt cggcttttca atataacctt cttcttctaa caatctctca aggttttgtt
    15421 ttaattcaag tttatcccct aaattatcga taacatgatc ggccattcgg ctttttttat
    15481 caatagaaat ttggctatag acacgtgctt tcgcatcttc taatgacaaa ttattacgtt
    15541 gcattaaacg atccatttgt atactttcag aagtgtatac aacccacact tcgtctactg
    15601 tattttccaa ttcattttca aataataatg gaatatccat gattacatta tatccttgtt
    15661 ttaaatattc ttgcttttct tcttccataa tatctcgcac gataggatgt atgatagcat
    15721 ttaattctaa gcgtttttct ggatgattaa acactagatc acccatataa cgacgattca
    15781 tctcaccatt ttcatcaatt gcttcatcac caaagacttc tcgtacttga gctaaacctt
    15841 tactcccctt tttaacagct tccctggctg ctttatcagc atctactact ttaaaaccga
    15901 atacggataa gagttctgat actgttgatt ttcctgaggc gattccacct gttagaccaa
    15961 taactttcgg cataatttca ctctttcttt atttttgaca tactggacaa taatgactat
    16021 ttcttgtcgc gatgattttt gtttcaattt gacttccaca cactttgcat accggctgct
    16081 tatatacatt aagatgcaat tgcatctcac cagtttttcc atcagcatga cgataatctg
    16141 aaatacttgt accgccatat ttaatacctt cttctagtac ttctctaaca taataaaaaa
    16201 ccatttcttg ttgttggtgt gttaagtctt ttactttttt atctggtaaa acacctgcac
    16261 gaaacaacgc ttcacatgcg taaatatttc cacaacctgc gattacttta tgatccaaaa
    16321 tcacttgttt gattggttta ttcttattag actgttgatg aattcgattt aaataatacg
    16381 tcaatgcttc atttgaaaaa ggttcaggcg ctatttctaa aaatgaagga taagatgcta
    16441 cagacgcaac atttctaatt tctccaaaac gacgtatatc tgaataaatt aactttttgt
    16501 catttgacaa ctcaaaaata acatgccaat gcttacgata attaggtatc ataatatctt
    16561 caagttcatc tacaatgaaa aaaccgcccg ccatacctaa atgactaatt aatgtacgtt
    16621 gttctcgttt attatctagc tgaaaaacga tatatttact tcttcgttct acatttgtaa
    16681 tggtatagcc ttccgataaa gttttaaaag tatctaattc aattcctttt ataattgttt
    16741 ccttgccttg agctttacct tcgattactt tatccgaaaa tataacgtgt tcaatttttt
    16801 gatttataac gtagggttca attcctcttt ttacatgttc tacttctggt aattcgggca
    16861 taccattaac ctcactttat tttgcatcat accaggttgc accataactt gagtctactt
    16921 ttaatggaac atctaattgc aatgcatttt ccattatctc ttctacaaat tcactaaatg
    16981 aatctacttc tgacttaggt acttcaaaaa ttaattcatc gtgtacttgt aataatagtt
    17041 tagcttgata tgttgtctct ttcatttttt gagcaaattt aaccattgcc agtttaatga
    17101 tatctgcagc gctgccctgt attggcgtat tcatagcagt acgttcagca aagccgcgta
    17161 aattaaagtt acaactcgta atatcaggaa tatagcgtcg acgatgtagc aatgtttcca
    17221 cgtaacctaa agctttggca tcttttacaa tatcagacat atattgtttt acacctggga
    17281 aactagctaa ataatcatca atgaatgctt ttgctttttt acgagtaata cctaaacttt
    17341 gacttaaacc ataatcactt atcccataaa caattccaaa gttaactgct tttgcttgac
    17401 gacgcattaa actatcgact tgatcagctt ctacaccaaa tactttcata gcagttgctg
    17461 tatgaatatc atcgccgttg ataaatgctt ctttcatact ctcatcttgt gtaatgtgtg
    17521 ctaatacacg caattcaatt tgagaataat ctgctgataa tataacgcta tcttttgaag
    17581 ttggtttaaa ggcttttctt attttacgcc cttcttcaag tctaaccgga atattttgta
    17641 aattaggatc tacacttgat aaacgtccag tttgcgctaa agtttgatta aaacgtgtat
    17701 ggatacgttg atcatcacta attacctttt gcaatccttc aacataagta gactgtaact
    17761 tagataattg acgatattct aaaatataat caatgatagg atgttcacct tgcaattgct
    17821 ctaagacatc tacagcagta gaatatcctg ttttcgtctt tttaataaca ggtaattgta
    17881 atgtctcaaa caatacaaca cctaattgct taggagaatt tatattaaaa tcttcaccag
    17941 ctgcatcatg gatatttcga atcaagacgt ctaatttttc ttgaatttct ttttccattt
    18001 cttctaaatc atgaacatct gtaaatatac caatttcttc catttcactt aaaatcttag
    18061 ctagcggtag ctctaaatca gccaagagtt ctacctgatt gtattcttct aattgtttat
    18121 ccatatttgg tttcgcaaag taaattgcat cagtaataga agcaacatat ggatttaaaa
    18181 catcatcttc aggtacctta aatttcttac cttttccata tatactcaca tcgtctttca
    18241 caaaactttg accgtacaat gaaacaactg attgaacatc actaatcgta cgagatggat
    18301 caattatata actggccaac ataatatcga aagaaatatt ttgaatatca atccccaatc
    18361 tatgtgatgc tacatatgtt tttttagcat catatacgac ttttttcgaa ttcggatttt
    18421 ctaaccatga aactagttcg acataattat ttatgtcatc cgcattaatt acaatatgtt
    18481 tctcacctgt aaataaagag aattttaaaa tattatttcg caaatagtta ccaccgtcta
    18541 attcgaaatg gatggccgct tctttcaatg aagtaaaatc aatattatca aaagacgttt
    18601 caatttcaaa tgtcttttct attgcatctt caacgcttgc tgattgatca atgtcagcca
    18661 acaattgttt gaattctaac ttcttaaaca attcgatttt ttcttgttgt tcatcttgat
    18721 gagtcattaa cgtatcttca agttttactt caatcggact atctacatta atcgttgcta
    18781 attctttact cattaatgca tcttctttgc tattttgaag tttttctttt aactttttac
    18841 ctgaaatttc atctaaatgt tcatagacac cttctactgt gtcaaattgg tttagcaatt
    18901 ttattgctgt tttctctcca acacctgcaa cacctggtat attatcagaa gtatctccca
    18961 ttaatccttt catatcaata atttgattag gtgttaaccc gttgtatttt tccgcaataa
    19021 agtcaggtgt ataatgatca acatcagtaa cacctttttt agtgtaataa atggttacat
    19081 tatccgttgc aagttgtgtt aaatctcggt ctcccgtaat aataattgtc tgaaagcccg
    19141 ctttatctgc ttctttactt aaagttccga taatatcatc tgcctcatag ttatctaatt
    19201 cataacgttt aatatgataa gcatctaata attggcgaat ataaggaaat tgctcactta
    19261 gttcaggcgg cgttttctgg cgtccacctt tatattcact atatttttca tgtctgaaag
    19321 tcgttttacc tgcatcaaac gctactaaaa aatgatttgg cttttcttct tttaaaatct
    19381 tctctagtaa cattgcaaaa ccatatactg cattggtatg aatgcctgct ttgtttgata
    19441 acaaaggtaa tgcataaaaa gctctaaaac ttaagctatt accatcgatt aatactaatt
    19501 tattcacaat tttaacctcc agaactaatt tatatatatt ttagcatatt cgactgtgat
    19561 gtcgtaatta aagaaactat ctcatagagt atttatacac gacctaattt caaataaaac
    19621 aagtgcctat atagacttag ctaaaaaata aaaaagacat aatcatgatt gttcttgatg
    19681 agcactccat aaagtcaggt taaaagtcca acttttgaga tgcacattat catttcatga
    19741 ttatgtctac tttttaatat ttttatgctg ctattttaaa tttttatagg taagaataag
    19801 ttatgaatcg tctgaaccaa atggaatata tttaactttg gtcattttcg gtaattcttc
    19861 ataatta
//
LOCUS       DAKTIP010000028        19578 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAKTIP010000028 DAKTIP010000000
VERSION     DAKTIP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.028"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            177..899
                     /locus_tag="O0305_000792"
     CDS             177..899
                     /locus_tag="O0305_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCY0273948.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            complement(1085..1360)
                     /locus_tag="O0305_000793"
     CDS             complement(1085..1360)
                     /locus_tag="O0305_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273949.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(1515..2579)
                     /locus_tag="O0305_000794"
     CDS             complement(1515..2579)
                     /locus_tag="O0305_000794"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCY0273950.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2797..3663
                     /locus_tag="O0305_000795"
     CDS             2797..3663
                     /locus_tag="O0305_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCY0273951.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRLDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            4005..4283
                     /locus_tag="O0305_000796"
     CDS             4005..4283
                     /locus_tag="O0305_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCY0273952.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            4298..4666
                     /locus_tag="O0305_000797"
     CDS             4298..4666
                     /locus_tag="O0305_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273953.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKVEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            4654..4950
                     /locus_tag="O0305_000798"
     CDS             4654..4950
                     /locus_tag="O0305_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCY0273954.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTQQ"
     gene            5284..6390
                     /locus_tag="O0305_000799"
     CDS             5284..6390
                     /locus_tag="O0305_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCY0273955.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            6384..7724
                     /locus_tag="O0305_000800"
     CDS             6384..7724
                     /locus_tag="O0305_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCY0273956.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            7822..8712
                     /locus_tag="O0305_000801"
     CDS             7822..8712
                     /locus_tag="O0305_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0273957.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(8859..9689)
                     /locus_tag="O0305_000802"
     CDS             complement(8859..9689)
                     /locus_tag="O0305_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0273958.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWLEFEIRQYTHSNITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETGIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(9709..10719)
                     /locus_tag="O0305_000803"
     CDS             complement(9709..10719)
                     /locus_tag="O0305_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0273959.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELSHHDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIA
                     QYPNRFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKT
                     AENLDVPIYLHPAPVNSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSG
                     IFDRYPKLNMIIGHWGEFIPFFLERMDEALFAEHLNHPVSYYFKNNFYITPSGMLTKP
                     QFDLVKKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGLSP
                     NN"
     gene            complement(10817..11521)
                     /locus_tag="O0305_000804"
     CDS             complement(10817..11521)
                     /locus_tag="O0305_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0273960.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            complement(11514..12071)
                     /locus_tag="O0305_000805"
     CDS             complement(11514..12071)
                     /locus_tag="O0305_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0273961.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDTYTKFKTDMLLQSLSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVGRND"
     gene            12202..12501
                     /locus_tag="O0305_000806"
     CDS             12202..12501
                     /locus_tag="O0305_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCY0273962.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            12521..13342
                     /locus_tag="O0305_000807"
     CDS             12521..13342
                     /locus_tag="O0305_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCY0273963.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            13359..13736
                     /locus_tag="O0305_000808"
     CDS             13359..13736
                     /locus_tag="O0305_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0273964.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(13886..14116)
                     /locus_tag="O0305_000809"
     CDS             complement(13886..14116)
                     /locus_tag="O0305_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0273965.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(14336..14836)
                     /locus_tag="O0305_000810"
     CDS             complement(14336..14836)
                     /locus_tag="O0305_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273966.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            complement(15034..15603)
                     /locus_tag="O0305_000811"
     CDS             complement(15034..15603)
                     /locus_tag="O0305_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0273967.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            15758..15886
                     /locus_tag="O0305_000812"
     CDS             15758..15886
                     /locus_tag="O0305_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273968.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSENSEFCDILFFIVLSYR"
     gene            16013..17068
                     /locus_tag="O0305_000813"
     CDS             16013..17068
                     /locus_tag="O0305_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0273969.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            17676..18377
                     /gene="isaA"
                     /locus_tag="O0305_000814"
     CDS             17676..18377
                     /gene="isaA"
                     /locus_tag="O0305_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCY0273970.1"
                     /translation="MKKTIMASSLAVALGVTGYAAGTGHQAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            18653..19141
                     /locus_tag="O0305_000815"
     CDS             18653..19141
                     /locus_tag="O0305_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0273971.1"
                     /translation="MAINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNNGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDKGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KD"
BASE COUNT         6591 a         2962 c         3554 g         6471 t
ORIGIN      
        1 cacagagacg tatgtgttgt ttagtgtttt aaaattcaag tttaaaaaat gcttttagat
       61 acataaggga ttttcgaaat ataaatgcat taaaatgaat ttctaacttt tttgactgac
      121 atttaatatg aatcatatat aatagaagtt aatgattaaa tgtgggaggt taaatcatga
      181 ttaatgctga acctattatc agtaaaatga aaaatcaaaa aattaattat gacaaggtgt
      241 tgaagaaatt aatcgggcag tgggaaagag aagcaatcag acctaaaatc ttattgcata
      301 gttgttgtgc accttgtagt acatatacat tagagttttt aacacaatat gcagacattg
      361 caatttattt cgcgaattca aatattcatc cgaaaaatga gtacttacga cgtgctaaag
      421 tgcaagaaca atttgtggaa gattttaatc gcaagactgg tgcgaatgtg aagtatattg
      481 aagcacctta tgaaccgcat aagtttgtga aaatggtgaa ggataaagaa ttagctgatg
      541 aaaaagaagg cggcttacgt tgtaccgctt gttttgaaat gcgtttggat attgtagcga
      601 aagcagctgt agaacatggc tatgattatt ttggcagtgc aatcacatta tcacctaaaa
      661 agaacgcaca attaatcaat gaacttggta tggatgtcca aaaaatatac gatgtgaact
      721 atttgccaag tgattttaag aaaagtaaag gttatgagcg ttccatagaa atgtgtaatg
      781 actataatat ctttaggcag tgttattgtg gatgtgtctt tgcagcgatg caacaaggta
      841 ttgattttaa aacagttaat aaagaagcga aagcattttt agaacaatat ccagattaat
      901 cattggaaat gagataaaag gcaagaacgt atacatgcca tatcccatta aaaattatca
      961 aatagaaacc cgagatttta gtgaaatgaa tcatactaaa atctcgggtt atttatataa
     1021 tcgtaaatat tgtagatata atgaataact ggatcctgag acataaaaca atgtctcact
     1081 ctaattacat cattttatga acaaatctta taattgctaa aatgacaatg acaagtgttg
     1141 aaataagtga aaacacttta aactttttat agtcattttt cgtttcattt tctcctgttt
     1201 tcatcatttc acgtagctct tgctcgattt ctaaatcaag atctgtttta cgataaccat
     1261 tcaaaccttg acgttcatct tcttcaggtt gattttgttg ctgtgtgtct ttgttgtcag
     1321 aagtcgctac tgttttttta ttatctgttt ctttagtcat aacaaacgcc tccgttataa
     1381 aacgctatat ttaatgatat gtgatattta agccattatt ttgaaaaatg gtgataaata
     1441 tatcttcttg agttatatca taattttaat aagacgattc agcaagtttt aaagtattat
     1501 ttgactatgt tggattaggc atctagtcct ataatatcac tgacattgtc aaaatgatga
     1561 tcttttaagt aacgtgcgat gcctttgttc attttcttag ttaaacctgg gccttcaata
     1621 acaagtgatg aataaatttg aataagtgac gcaccgtgac gcatcatttt gattgcatct
     1681 tcagtactga atacgccgcc tgtacctata attaaaaatt caccatttgt ttgctgataa
     1741 gcatacttaa tcaattttaa attacgttca aataatggac gaccactcaa accgccttct
     1801 tcgactttat tagcagaagt taaaccatct cgttgtcgcg ttgtgtttgc taagatgata
     1861 ccgtcaaatg tctcagtaat cgctggtaat agtgctttta agccatcgaa atccatatca
     1921 gacgttagtt ttaaataaat tggcactgtt acatcatgtt gttttttaaa tgctgttaaa
     1981 gcttggcata acattgaaaa ttcatcttta tcatggaagt tttgaagatt ttcagtattt
     2041 ggagaactga tgttgactgt gaaaaatgaa acgtcgtgtt taaacgtatc aataaccttt
     2101 atataatctt gataacgcgc ttcataaggt gtcattttat tcacaccaac attgatacca
     2161 acaggtactt gataagcatt tttacgcaaa tgacttagtg ctttgttcat accaatatta
     2221 ttgaagccca ttcgatttat caaggcgtca tcttctaata atctaaacat gcgtggttga
     2281 gggttacccg gttgaggttt aggtgtgata ccacctaatt ctaaagcacc gaatccaagg
     2341 tgttccaatg cttttggtac ttcgcaagat ttgtcgaaac cagctgctaa gccaattgga
     2401 ttgtcgtacg tattaccttg tatcgtttgt gataacgttg gattcttata agtaaatagt
     2461 ttatcgacga ctgggaataa aaccggaaac ttttgtaacg tttttaatgc atcgatagtt
     2521 agtccgtgtg ctttttcggg ttcgattttg aataagaaag gtttaattaa tttgtacatg
     2581 agtatgctcc tatttcatta tatttgaggc ttactatcct caacttaata tatgtgaaat
     2641 atattctttt aatagactag catttccata cataatttcc tagttaaaac taaaaagttt
     2701 tgaaaattga cgcaagtttg aataacgttt ttaagattaa atcatcctaa ttaggcaata
     2761 ttatagtata aagtaagtag attggaaggt gtttgtatga atgaacaatg gttagagcat
     2821 ttacctttaa aagatattaa agagatttca ccagtgagtg gtggtgatgt aaacgaagca
     2881 tatcgagtcg aaacagatac ggatacattt ttcttacttg tccaacgtgg acgtaaagaa
     2941 tcattttatg ctgcagaaat tgcaggttta aatgaatttg aacgtgcagg tatcacggca
     3001 cctagagtaa ttgcaagtgg cgaggttaac ggtgatgcgt atttagtgat gacgtattta
     3061 gaagaagggg cttcagggag tcaacgccaa ttagggcaac tcgtagctca attacacagt
     3121 cagcaacaag aagaaggcaa atttggcttc tcattacctt atgaaggtgg cgatatttct
     3181 tttgataatc attggcaaga cgattggtgt accatttttg tcgacaaacg cttggaccat
     3241 ttgaaagatg agttgctaaa tagagggcta tgggatgcca acgatatcaa agtatatgac
     3301 aaagtgcgac gtcaaattgt ggcggaatta gaaaagcatc aaagtaaacc gtctttatta
     3361 catggtgacc tatggggtgg taattatatg ttcttacaag atggtcgtcc ggcgttattt
     3421 gatccagcgc cattatatgg tgacagagaa ttcgatatcg gtattacaac ggtatttggt
     3481 ggttttacga gcgaatttta tgatgcgtat aataaacatt atccactcgc aaaaggtgca
     3541 tcctatagac ttgaatttta tcgtttatat ttattgatgg tccatttatt gaaatttggt
     3601 gagatgtacc gtgatagtgt tgcgcattct atggataaga ttttacaaga tacaacaagt
     3661 tagttaagac gttagattga gataaataga taatatgcac agatattttt acaatgagaa
     3721 gcgatacagc tgcctcaata aaaatatttg tgcgttttta ttgttggaaa ataaaatttt
     3781 aatcgctatt gttaatttct gtaatgtaaa acaaggttga gttacaataa aagtgatttt
     3841 ataacttttt gttcaataaa attctaggaa tgatacatat ttattgatac aataattttg
     3901 aatataatca taaaacaata tttaagtata attgaatgtt tgaatatcat atattgatac
     3961 agtttctaat aattttaaaa taatttaaat ggagagaggt gtaaatgatg agtacagttc
     4021 aaagtgatat ttttaagacc aatagtgcat catcatctat taaaagcgct gttgaaacat
     4081 gtaataatgt gtcgaaaccg gataaagatg aaagtacaac agtaagtgga aataataatg
     4141 ctcatagtgt gatagatgat ttgatgagta agaatcaatc tgttgctgaa gcaatacgaa
     4201 ctgcgagcga taatatacaa aaagttggtg aggcttttga ccaaactgac gtaatgattg
     4261 gtaatgaaat tggtaaaaat taaaacgtgg tgaaatgatg tcgaataaac tggatgaaat
     4321 caataaaata atcacagcga aacatgagca aatggatgac ttatatgatg aaaagcgaga
     4381 ggttaaagca ttgatagatg aaagtgatgc gcttaatcat tcgatagatc aattatatca
     4441 acatttaggt gagcgttatt atagtagcaa tatggctagt cgtatggaac agttccgcga
     4501 tgaatttcat tttgcgaaac gacgttcaac ggaagcgtta tacgagcagc aacagcaaat
     4561 tcaacatggc attcgtaaag tggaagaaga gatgattgac ttggaaatgc gaaggaatgt
     4621 tgaaattgag acggtgacaa aggaggaaaa taaatggaaa caataggaag cattatttat
     4681 ttaaaagaag gttcgcaaaa gttaatgatt attaatagag gaccaattgt agaaattgaa
     4741 aatcaaaagt atatgtttga ctattctgca tgtaaatatc cgattggtgt tgtagaagat
     4801 gaaatttatt attttaacga ggaaaatata gattcagtta tttttaaagg ttattctgat
     4861 caagatgagg ttagatttca agagttgttt gaaaatatga aacaaaattt ggatagtgaa
     4921 atacaacgtg gagaagttac acaacaataa agaaatactt tttctttatt ggggtgggac
     4981 gacgaaataa attttgtaaa aatatgattt ctgtctcacc gccaaaatgt tatctccaag
     5041 tactgaggct ggatgatacc atgaaatgtc attcataatc atttgaacta aacgtagcag
     5101 ccttaaattt taaaaaaaga cacataccaa cttccgaaat gtagatgaat tctctacaat
     5161 aacggaagtt tttcttttaa tattgaaatt tctcaaggat aggtctatac tttataaatc
     5221 gtaattatta cgatttataa tcaaaaacaa taacttgaaa tagatcattg agggagtgtt
     5281 aatatgcaac atcataaagt ggctattatc ggtgccggtg ctgcaggtat aggtatggcc
     5341 attaccttaa aagatttcgg tataacagat gtcattattt tagaaaaagg aacagtagga
     5401 cattcattta aacattggcc gaaatcgacc cgtacgatca cgccatcatt tacgtctaat
     5461 ggatttggca tgcctgatat gaatgcaatt tccatggata cttcaccagc atttacattt
     5521 aatgaagaac atatttccgg agaaacatat gctgaatatt tacaagtggt tgccaaccat
     5581 tacgagctga atatctttga aaatacagtt gtcacaaata tatctgtaga tgatgcatat
     5641 tatacgattg caacgacaac agagatatat cacgcggatt atatctttgt cgcaacaggt
     5701 gattataatt tccctaaaaa gccatttaaa tatggtattc attatagtga aattgaagac
     5761 tttgataact ttaataaggg gcaatatgtg gttatcggag gtaatgaaag tggctttgat
     5821 gctgcatatc aacttgcaaa aaatggctct gacatcgcac tttatactag cacaaccggt
     5881 ttaaatgatc cggatgctga tcctagtgtt agattgtcac cttatacacg tcagcgacta
     5941 ggtaatgtca ttaagcaagg tgctcgcatc gaaatgaatg tacattatac agttaaagat
     6001 attgatttta acaatggaca gtatcatatc agttttgata gcggacaaag tgtgcttaca
     6061 cctcatgaac caatactagc aactggcttt gatgcaacaa aaaatccaat cgttcaacaa
     6121 ttatttgtga caacaaatca agatattaaa ttaacaacac atgatgaatc gacacgttat
     6181 ccgaatattt ttatgattgg tgcaacagtt gaaaatgata atgccaaatt atgctatatc
     6241 tataaattta gagcgcgatt tgcagtactt gcacatcttt taacacagcg ggaaggctta
     6301 ccagctaaac aagatgtcat tgaaaattat caaaaaaatc aaatgtattt agatgattat
     6361 tcatgttgtg aagtgtcatg cacatgttag aagtgaaata tgatatgaga actgggcatt
     6421 atacgcccat acctaatgaa cctcattatt tggttattag tcatgcggat aaacttaccg
     6481 caacagaaaa agcgaaatta agattattaa tcataaaaca gaaattagat atttcattgg
     6541 cagaaagtgt agtttcttcg cctatagcga gtgaacatgt gatagaacaa ttgacactat
     6601 ttcaacatga gcgacgacat ttaagaccta aaataagtgc gacattttta gcctggttgt
     6661 tgatattttt aatgtttgca ttgccaatcg gtatcgctta tcaattttca gattggtttc
     6721 aaaatcagta tgtgtcagca tggatagaat atttaactca aacaacattg ctcaatcacg
     6781 atatattaca gcatatatta tttggtgatt atggtgtgct atcacttgga acatattcgc
     6841 tcgtatgggc attgccggtt gtaatattga ttagtttatc aactgctata attgatcaaa
     6901 caggactcaa atcatggatg atatgggcaa ttgaaccgtc aatgttatgg ataggattac
     6961 aaggtaatga tatcgtgcca ctattagaag ggtttggatg taatgcagca gctatttcac
     7021 aagcagcaca ccaatgccat acctgcacga agacacagtg tatgagttta ataagctttg
     7081 gtagttcttg tagttatcaa ataggtgcga cattatctat ttttagtgta gctggaaagt
     7141 catggctatt tatgccgtac ttaatattag tacttttagg tggcatctta cataatagga
     7201 tatggttgaa aaagaatgat caacaactta gcgttccgct accttatgat aggcaattac
     7261 atatgccaaa tatacgtcaa atgttgctac aaatgtggca aaatatacaa atgtttatcg
     7321 ttcaagcgct acctattttt atcacaatct gtcttattgt tagtatttta tcactaacgc
     7381 caattttgaa tgttttatca caaatattta cacctatatt atcgttatta ggcatctcgt
     7441 cagaattgtc accagggatt ttattttcaa tgattcgaaa agacggcatg ctcttgttta
     7501 atttgcatca gggcgcctta ttacaaggaa tgacagcaac acagttacta ctacttgtgt
     7561 tttttagttc aacatttaca gcgtgctcgg tcacaatgac gatgcttttg aaacatttag
     7621 gtggtcagtc agcactaaaa ttaattggaa agcaaatggt gacatcattg tctttagtta
     7681 ttggtgtagg catcattgtt aaaatagtaa tgctgattat ttaaaaaaaa tgaactataa
     7741 ctgaatatag agtcatgtca gtcaatagga gatctatctt ggaatatgct attcatatga
     7801 agtataagag gagagtcgca gatgaaaata gttattatag gtgggttttt aggtggcggt
     7861 aaaacgactg tcttaaatca tttgctcgct gaatcattaa aggaatcgct gaaaccagca
     7921 gtcatcatga atgaatttgg gaaaatgagt gttgatggtg ccttagtatc tgaagacata
     7981 cctttaagtg aactgacaga ggggtgtatc tgttgtgcaa tgaaagcaga tgtatcagaa
     8041 cagttacatc aattatattt aaaagagcaa ccagacattg tatttattga atgtagtggg
     8101 attgcagaac cggtctctgt cttagatgct tgtttaacgc ctattttagc tccgtttaca
     8161 acaattacac atatgattgg tgtaatagac gcaagcatgt ataaacacat taaatcattc
     8221 cctaaagaca tccaaggctt attttatgag caattagcat attgttctgt cttatttgtt
     8281 aataaaatag attcagcaga tgttgaaaca acgagcaaac tattgaaaga tttagaagtt
     8341 attaacccag aggccgatat acaagtcggt atgcatggca gcgtcacttt gccaatatca
     8401 gttagacaaa tgacagcaac ttctgacaat aaacataagt ctttacatca aatgattaat
     8461 catcaatttg tgcaatcacc agtcaaatgt actaaagcag agtttataaa acgtttagca
     8521 tgccttccgt ctcatattta taggttgaaa gggtttatga catttgaaga caccgcacat
     8581 acgtatctca ttcaatttac acaaggacaa tatgaattaa cacctgtagc attttcaaaa
     8641 aaagtgccag aatatttggt gcttatcgga aagggtattt caaaagaaga ctatcaatgt
     8701 ttggaacagt agtgttttca gtggaagaga atggttaaca tgccttcatg tataataacg
     8761 agttgatttg aacgtttaag cgtaaataaa aataagcttg gtcagccatc aaatataatt
     8821 tgaaaactgt ccaagctgtt ttattagaga acaatcaatt aaccccacat atttaataat
     8881 acatcagcaa agccttcagg tttttgaata taacctaagt gaccgcctgg aatatctaca
     8941 ataggtatgc cagtttcttt atttatataa aagttaacat cttgtgggaa ggagcctcta
     9001 gaatctgtcc catttagtag ggtgatttta tcgctgtatt ttgtgaaatc atccaaagta
     9061 atatttgaat gcgtatattg tctaatttca aattctaacc agaacatcgt acgtttgtac
     9121 tgttctatac gtccttcttc agtatcagca ggttgagaca tcatttttgc atcaattggt
     9181 gcgatattta atgtttcgcc aaatgttttc atgccttttt ctaagccttc tgttaaaatt
     9241 tgatgcacaa tgtcatcatt tttatctttc caataagtac tgtctggtaa aaatgtatta
     9301 attggtggtt cgtgaaatgc aatcttttta acgacttcag ggtaatcttt taacacatgc
     9361 atcgcaacga ttgaacctga acttgaacct aatatataga caggttcatc acttaatgac
     9421 tttgcaagtt cggcaatgtc ctgtgcgtcg cgtttgacac gataatcact gtcagggttt
     9481 gaagcggaat cagggagtgg ttcagttaac tcgctttctc cataatcacg acgatcaacg
     9541 gctacaacag taaaatggtc ttttaactgt tctgcaagag gcagaaaaat gtctccggta
     9601 ccgtttgcac caggaataaa gatgagcacg ggtccttgtc cgacttggtg gtatcgtaat
     9661 ttagcgcctt gtaattctaa agtttccata ttcaatgacc tccatttgtt aattgttagg
     9721 tgataaacct aataatttag caccatttgt ataacttatt ttctcttttt cttcatctgt
     9781 taaacccagt tcatctaaaa atacacctaa tttttcaggc tcaatatatg gataatcagc
     9841 agcataaaga attctatcaa tacctacttc tttcttgact aaatcaaact gtggcttcgt
     9901 taacatgcca ctcggtgtga tataaaaatt atttttaaag taatagctta cagggtggtt
     9961 caaatgttca gcgaataaag cttcatccat acgttctaag aagaatggga taaactcacc
    10021 ccaatgtcca ataatcatat ttaactttgg ataacgatca aaaataccag ataatactag
    10081 atgtattgca tgaatgccga catcaatgtg ccaaccataa ccaaaacaag caaatgttgc
    10141 cgcagttact tcaggataat ttcctttata gtatgattga taaatgtcac tgttaactgg
    10201 cgcgggatgt agataaatcg gtacgtctaa attttcagct gttttgaaaa taatgtcata
    10261 tttgtcttga tcaagaaaac catcttgtgc acgtcccata atgagcgcac ctttgaatcc
    10321 taaatcattg atgcaacgtt cgaattctcg cgctgcggct tcaggctcat tgataggtaa
    10381 agttgcaaag cctacaaagc gattgggata ttgtgcaata tagtttgcca attgatcatt
    10441 tgctttttga cataattcaa tggctttctg accaaccaaa tttgaaggag aaccatttcc
    10501 ataagataag acttgaattt gaacgtcttg attattcata aattggatac gttcatcatg
    10561 atgtgataat tcgtcggcat ttgtaaaacc tgtctttttt tcaaggcctt ctaacattac
    10621 tttcatcggt acacctttag gatctgctga tatcgcattc atcgtttctt tttgaatatc
    10681 ttcaatgaca taatgttctt caaacgtaat acttttcatt tacttcgcct ccatattgta
    10741 ttgcatgttt attgcatcta ttgcagaagc attttttata tacctctaat ttcaatgttt
    10801 gtaacataaa attgatctac caaggcatct ctccatcgcc attaataaat gtacctgttg
    10861 ggccatctgc accaatcgtt gctaattgaa tgattggctt gattccttca gaaacgtgtt
    10921 tggaattatt actaaaatca ccaactaaat cagtatttgt agcgcctgga tcagcagcat
    10981 tgatttgcat gttaggtaat cctttagcgt attgtagcgt tagcattgtt actgccgatt
    11041 tagacgaaca ataagctaat gaattcactt tagattcagc tgtttcgggg tttgtaacca
    11101 ttccaaatga acctaaacca cttgatacgt tgacgacaac aggttgttca gatttttcta
    11161 agagagggac gaatgtattc atcattcgta cgataccgaa tacattcgtt tgatatactt
    11221 cttcaacgtc acgaggtgtc aatttggaag gtgctgaaaa ttgaccagat atacctgcat
    11281 tgttaatgag gatatcaaga cggccttctt tttcagcaat catgttataa gcatttttga
    11341 ctgagtagtc acttgtaaca tctaattgta cataatgaac acctaatttt tgtgatgctt
    11401 gttgtcctct tacatcattc cgagaaccta tataaacttt gtaacccaat gctttaagtg
    11461 cctctgcact tgcatagcct aaccctttat tgcctcctgt gattaacaca attttagtca
    11521 ttacgtccca cctcatctaa ataaatgttt aataaataat ttctgtacgc ttcaattgaa
    11581 atatggcgat gctctatttg gaaggcaaat acactagttg ataatgattg caacagcata
    11641 tctgttttga acttcgtgta agtgtcgtca tcgcttttaa ataagtcata ataaaaatca
    11701 aataattctt gataaaatgc gctttggtaa aaacgtaatt tattgttgcc tgcttcaata
    11761 cattgcagta gtgccttatt atcgatttta aattgtaaaa gataatctaa cgacacttgc
    11821 ataacctcat aattagaatg atagtcatct ttaatttgct taaaatgagt gataaaaata
    11881 tcaaggtctc tttgtatgac gtagtagcat aaatcgcttt tatctttgaa atgtcgatac
    11941 aatgtcccca taccgatacc tagttcttta gcaatacgat tcatactaat gttttcaacg
    12001 ccttcttcat caaaaagttt gtgcgctatt tcttcaattc gttgcctatt ctcttttgca
    12061 tcttttcgca tgattacacc tacttaaaat tctctaaaat tgacaaacgg ataactctcc
    12121 gtttattata aaacgtgtta agaaagttag caatgaattt gcaataacta ttaaatatca
    12181 taaaagaaaa gagtgttgat aatgtcttta aataaagagc aaagacgcat cacagctgaa
    12241 gagttgcaag cacatttcga agcatctacc ttatcggttc aaatgattgc tgaaaaactg
    12301 aatgtcacta cagaagatgt ggaaaaagta ttagctatga cagcgccact aggcattttt
    12361 agtcatcaat tacaacgatt tattcattta gtatgggatg tcagagatgt aataaacgac
    12421 aatattaaag gaaatggaca aacaccagaa ccatatacgt atttaaaagg tgaaaaagag
    12481 gactattggt ttttaagata aaaagaggag tggatctaaa atgaaagata ttttagtaat
    12541 aggcgcaaca gggaagcaag gtaatgcagt tgtaaaacag ttgttagagg atggatggta
    12601 tgtaagtgca ttaactagaa ataagaataa tcgtaaactt tcagacatag gacatccgca
    12661 tttatcaatc gttgaaggtg atctgagtga taatgttagt ttgcaatcag cgatgaaagg
    12721 taagtatggc ttatacagta ttcagccaat cgttaaagat gatgttagcg aagaattaag
    12781 acaaggtatg aagataattg aaatagctga gcaagaaaat attcaacata ttgtgtatag
    12841 tactgcggga ggggttaatc gaaatcgaac tggcccacac tttgaagtac tagcaaaaat
    12901 agaaaataga ttaatggaat ctaatataaa tgcaacagtt atcaaaccat cattctttat
    12961 ggataatttt ttacgcattg ctaaagtcga agatgaacgt attacattac cagaatttat
    13021 taatccgaat ataaaattca caatgatttc ttctattgat atagctaaaa ttgcatcgta
    13081 tatttttgca catccacaaa gctttacgca tcaatcaata gagattggtt ccgatgaagt
    13141 tacattaagt gaagcagcaa caatttttag tgaagtgaca ggtaaatcta ctgttataga
    13201 aggagaattt gttagtggtg ttgcagaaaa gcaatggttg gaagaaaaag gttatgaagt
    13261 agactttgaa ttaatggctg aaataaatcc aacaagatta tcattaagtg attggcttaa
    13321 agtccaaaac tataataagt aatgaatggg gtgcaatcat gattcaatca atgtggttta
    13381 atttgcatgt gcaagattta gaaaagagcg cacagtttta taaagcgtta ggatttaaaa
    13441 taaatagaaa cccacaaatg ttagataaaa tggtcggcat tcaaataggt caaacaaccg
    13501 taattttaat agaaaacaag cattttcaaa atgtaagtca gcaaagcctt aatactgaac
    13561 caaatgaagt gatgatttct ctaggtgtga acacaaatga agaagttgac cagttggtga
    13621 ataaagtgaa agaagcgggt ggcacagtcg ttcaagaacc aacagtaagc caagggtttt
    13681 atggagcaat gttcaaagat cttgatggac accattttaa ttttttggtc tgctaaaatg
    13741 aaattctcaa aagaataaat aaaaagccca tacagagatg aagcgtaatg tgcaccatgg
    13801 tgcgacacat tagtgtcttc aatcagtatg ggctttgttt atgaggtcag ttggcacctt
    13861 aagtgcaata cctcgtgagc aaatattaat tttgaatata accgtattgt tggcgaagat
    13921 cataaacttt atctggtgtg acatcctgac cgattggatc aaccactttt attgtggaaa
    13981 atgttgttaa tacttgtcct cggatcatgc ttaagtagtc ttgacgcaat gcatgttgtt
    14041 cttgtttttc agctacagta agtggttgta ctttttcttt atttgcaagt tcattaattc
    14101 tatctaaaat tttcatattg tcatatcctc cttagaatca agtgttgata tattttgaga
    14161 ttatttttat atggtaattt atcaacacat taagtaatgt ttttagtttc tcggtctttt
    14221 gaccagcaga tttttggaat taaaataagg gatttgagac acgattttac tagtagtacg
    14281 atggcaaagt gatgttattg aagtgtcgtt tgatatacat gtctgcaatg ttgttttata
    14341 agacaggatg acgtaactga gcaaagaaat gagcattgtc gaatgacatc gtgtgtctta
    14401 ttaaaattgc aatgggcata gcataggtat attcacagtc gattttcttg atttctacat
    14461 tatcataata cgtagtgtaa aagttatacg acagtcgata gtcgattcgc tttttaccac
    14521 gagcaattat tatgtgttgc ttcaacgtcg actttaattt atttgtaatg tatgtcacta
    14581 cacccagact actctgatta atatttaata tattatgcgt gcgttcaaac ccctgaggtt
    14641 catctgtctc gctcacgtca atccctccta tcttagtatt taaacaacgc atatcaacta
    14701 tgcaacatat gttgtttaga catactatac tactatgtcg tacgtttgcc aataaacacg
    14761 cacatatcat ttgttgccta aacaacatat tgcctgaagt gttgtttaag tacgcaaaaa
    14821 caaaaatgct tgtcactaaa atctatatgt tttaatgaca agcttatttt cattcagtta
    14881 acgactgcta atatgacatt gtagagtata ggacgttgat ttatgtccta gacacggcta
    14941 atcaatcgta atacgttaaa ttttattgtg tgtacaagta tgaacctaag tcacatagtg
    15001 atttcaagat gcatgactaa ataccaagtt ttattaaaaa tgtgctatat gatgaattaa
    15061 aaatggaagg ttgcgatcga taaacacatc ggctgtctct tgactcgtgt taaaccaatg
    15121 taatgctaag cctgagatac cagctgctaa aaaactcgca ctatttacaa tgatatctcg
    15181 ttgatgctcc ggatagctgt tttctaacat aatcgcaaaa atatcttcta attcattatt
    15241 aatacgttga tgtgctttgt tgcaaaatgc ttcactattt aattcgcaag actttgcagc
    15301 atctttgata taactcgcaa ttgaaatgaa aatattacgc agaacctttt cattaatgac
    15361 attagaaatg ctcaaattat cattcaagtc ttttaaaatg gttacagata atgtgtagtc
    15421 taggaggtct tctttatcag tgaaatgagc gtaaaatgtt gcacgattta gcgtagcaat
    15481 atcagtgata tctttaactg taatttgaga taatttcttt tctctagaaa ttttaagaaa
    15541 agcatcgaca agcaattgtt tggttctaac gattcttgga tctatggatt tactttccat
    15601 catttgttca ctacctaaca tctgttgttt taacacttat tatcagtatc ttctattata
    15661 ttgtcaattg tgttgatgat atgtaagtgc tgatacgctc ctaacaaata atgtaccttt
    15721 ttggtaagca tttgaatgtc tttcttctat tgtattaatg gattttaaaa atgaagataa
    15781 aataaaagtg tattgtaaca atatgtttga aaaattgcca atttctttat ctgaaaattc
    15841 agaattttgt gatatactat ttttcatagt cttgagttat agatgatact gctagcctaa
    15901 atataacaac atcggcatgc acatcttaga atttaagcta gtggcagctt ataacttaag
    15961 gctctttatt atttgcatac agatgtatat acataaatgg aaggatgata ttatgacatc
    16021 aattaaaaca attacaccca aagattttat atttagagtt ttatcaggtg tcgccatagg
    16081 tattgttgcg ggtttagttc ctaatgcgat acttggagaa atttttaaat actttatgga
    16141 ttatcatccg atttttaaaa cgttattagg cgttgtcgtt gcaattcaat ttacagtacc
    16201 tgctttgatt ggtgcattgg ttgccatgaa atttgaccta tcgccattag caattgctgt
    16261 tgtggcaagt gcagcatatg taggaagcgg tgcagcgcag tttaaaaatg gtgcttggat
    16321 gattacgggt attggtgatt taattaatac aatgattaca gcagcgattg cagtgttatt
    16381 cattttactt attcaacatc gcgtaggtag tatggcattg attgtattcc caacagttgt
    16441 tggcggtatt tcaggtgcaa ttggtgtttt aatattgccg tatacgaaaa tgattacaac
    16501 agccatcggt aatatggtta atggttttac agaattacaa ccgattgtca tgagtatttt
    16561 gatttcaatg gtatttagtc ttattattat ttcgccacta tcaactgtgg ccattgcctt
    16621 tgcgataggt attacaggtt tagcagctgg atcagcgtca attggtatct ccgcgactga
    16681 agcagtattg ataattggta caagtaaagt gaatcgttta ggggtgccgt tatccgtctt
    16741 ctttggaggc gttaaaatga tgatacctaa tatggtgaaa tatccaattc ttatgttacc
    16801 aatattaacg acagccattg tatcaggttt ggtatcagca ttagtaggta ttcatggaac
    16861 gaaagagtca gctggatttg gttttatagg aatggtagga ccgattaatg catttaaatt
    16921 tatggaggtt gattctgctt ggttgagtgt cttactaatt gtagtagcgt tctttgttgt
    16981 accatttgta acagcttggc ttgcagatat catttatcgt aaagtgtttc gtttatatac
    17041 taatgatatt tttaaattca tgggataata acagtatgtt ttttgaaaat atgagaccaa
    17101 aaattattta atgttattgt gaattgcgag aatgaagttg aacaatataa ttgagctgca
    17161 gtttatttgc gctaataaaa gataagacaa ttcacaaaag atgtgtgttt taaaactttg
    17221 aatatacaac aataatagca atcaatacat gtgtagaaac atatttgttg attgcttttt
    17281 aattgcgttt aatgtacgaa aataagagaa gttacatgtc agatatatta cagctatgta
    17341 acaaaaatac aatctgtaat attacgaaag ctgtaatagt tgagctaaat atagtaaaaa
    17401 tcttatgtgt taaagactat ttcaagggtt gattggattc taaagggcac atatttcaca
    17461 ttacagtata aaacagccat atttcaacat tacaaataac acttgatatt gtaatgtttt
    17521 gtaaagaaag tgtaatttac tggctggttt tttgtgatat agtgagtacc gttgaaagaa
    17581 ataaaagaca atttaaactt gcactacgac attcaaattc aaaatataat tactaaaaac
    17641 ataaacttac aattattact ggaggatttt ttactatgaa aaagacaatt atggcatcat
    17701 cattagcagt ggcattaggt gtaacaggtt acgcagcagg tacaggacat caagcacacg
    17761 ctgctgaagt aaacgttgat caagcacact tagttgactt agcgcataat caccaagatc
    17821 aattaaatgc agctccaatc aaagatggtg catatgacat ccactttgta aaagatggtt
    17881 tccaatataa cttcacttca aatggtacta catggtcatg gagctatgaa gcagctaatg
    17941 gtcaaactgc tggtttctca aacgttgcag gtgcagacta cactacttca tacaaccaag
    18001 gttcaaatgt acaatcagta agctacaatg cacaatcaag taactcaaac gttgaagctg
    18061 tttcagctcc aacttaccat aactacagca cttcaactac ttcaagttca gtgagattaa
    18121 gcaatggtaa tactgcaggt gctactggtt catcagcagc tcaaatcatg gctcaacgta
    18181 ctggtgtttc agcttctaca tgggctgcaa tcatcgctcg tgaatcaaat ggtcaagtaa
    18241 atgcttacaa cccatcaggt gcttcaggtt tattccaaac tatgccaggt tggggtccaa
    18301 caaacactgt tgaccaacaa atcaacgcag ctgttaaagc atacaaagca caaggtttag
    18361 gtgcttgggg attctaattc cttcataata aataaaagta atgtttagac attaaatata
    18421 tagctcttcc catagaaatt ggggagagtt tttttattaa ttaaaaatac attttttaaa
    18481 aagtggatat tgaagttagg gaaatttatt ggactttatt gtattactgc taaaatactt
    18541 gtctttatta agtgccaagt ggaatgaaac ttcatttgag gctgtactga gtacactaga
    18601 tacaaactaa acgcacattt tataattaaa ttaaatgaaa aaggagataa aaatggctat
    18661 aaacataatt gaatataata ggtcttataa ggaagagcta atcgagttta ttttatcgat
    18721 tcaaaaaaat gaatttaata taaaaattga tagagatgat caacctgatt tggagaacat
    18781 cgaacataac tatttgaata atggagggca attttggctt gcaataaaca atcatcaaaa
    18841 tattgttggt actataggtt tgattagact agataataat atgtctgcac ttaaaaaaat
    18901 gtttgttgat aaaggttata ggaatttaaa aataggtaaa aaacttttag ataaagttat
    18961 aatgacatgt aaagaacaaa atatcgatgg tatttattta ggaacaattg ataaatttat
    19021 tagtgcacag tatttttatt ctaataatgg cttccgtgaa attaagcgag gagatttacc
    19081 aagtagtttt cctaaattag atgtagataa taggttttat tacagaaatt taaaagatta
    19141 attcgatgca ataataattg atatgacaat cttagtaata ttcattttaa aagtgacaca
    19201 tagcgtttaa atataaagtc tgacgagata tatcgtttgg tttacattag aggataggct
    19261 gcatttcgct tttttttcaa tacatgttaa tttattcatt atataaattt tgaaaaagta
    19321 tattcgaata gcatatctta atgattctgt tacagtaata tgcataaaat ataaaacgaa
    19381 ccttttttgt aaaaatataa tgaaaataac agttattttg ctaaaaattc aagaatctat
    19441 gataatctat gttatgtagt aaaatcgaca aatttatatg tggtttgcag taatgaaatc
    19501 aactgtaata attgttttct acttaataaa aaatttaaaa ctatcttaat taagggttaa
    19561 atcaattaat gaaaatca
//
LOCUS       DAKTIP010000029        19020 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAKTIP010000029 DAKTIP010000000
VERSION     DAKTIP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.029"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            55..930
                     /gene="rsgA"
                     /locus_tag="O0305_000816"
     CDS             55..930
                     /gene="rsgA"
                     /locus_tag="O0305_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCY0273972.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            931..1575
                     /locus_tag="O0305_000817"
     CDS             931..1575
                     /locus_tag="O0305_000817"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCY0273973.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            1582..2223
                     /locus_tag="O0305_000818"
     CDS             1582..2223
                     /locus_tag="O0305_000818"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCY0273974.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(2604..2792)
                     /locus_tag="O0305_000819"
     CDS             complement(2604..2792)
                     /locus_tag="O0305_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCY0273975.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            3235..3609
                     /locus_tag="O0305_000820"
     CDS             3235..3609
                     /locus_tag="O0305_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0273976.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            3624..5270
                     /gene="fakA"
                     /locus_tag="O0305_000821"
     CDS             3624..5270
                     /gene="fakA"
                     /locus_tag="O0305_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCY0273977.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            5460..7520
                     /gene="recG"
                     /locus_tag="O0305_000822"
     CDS             5460..7520
                     /gene="recG"
                     /locus_tag="O0305_000822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCY0273978.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEINVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            7723..8295
                     /gene="fapR"
                     /locus_tag="O0305_000823"
     CDS             7723..8295
                     /gene="fapR"
                     /locus_tag="O0305_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCY0273979.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            8300..9286
                     /gene="plsX"
                     /locus_tag="O0305_000824"
     CDS             8300..9286
                     /gene="plsX"
                     /locus_tag="O0305_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCY0273980.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            9279..10205
                     /gene="fabD"
                     /locus_tag="O0305_000825"
     CDS             9279..10205
                     /gene="fabD"
                     /locus_tag="O0305_000825"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCY0273981.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            10198..10932
                     /gene="fabG"
                     /locus_tag="O0305_000826"
     CDS             10198..10932
                     /gene="fabG"
                     /locus_tag="O0305_000826"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCY0273982.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            11175..11408
                     /locus_tag="O0305_000827"
     CDS             11175..11408
                     /locus_tag="O0305_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCY0273983.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            11524..12255
                     /locus_tag="O0305_000828"
     CDS             11524..12255
                     /locus_tag="O0305_000828"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCY0273984.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            12402..15968
                     /gene="smc"
                     /locus_tag="O0305_000829"
     CDS             12402..15968
                     /gene="smc"
                     /locus_tag="O0305_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCY0273985.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIYGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETYLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            15968..17218
                     /gene="ftsY"
                     /locus_tag="O0305_000830"
     CDS             15968..17218
                     /gene="ftsY"
                     /locus_tag="O0305_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCY0273986.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            17205..17537
                     /locus_tag="O0305_000831"
     CDS             17205..17537
                     /locus_tag="O0305_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCY0273987.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            17563..18930
                     /gene="ffh"
                     /locus_tag="O0305_000832"
     CDS             17563..18930
                     /gene="ffh"
                     /locus_tag="O0305_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCY0273988.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
BASE COUNT         7105 a         2497 c         3762 g         5656 t
ORIGIN      
        1 attttccagt ctttgatata ataaatgcag taaaattaat cgagaggtgc cattttgaag
       61 acaggtcgaa tagtgaaatc aattagtggg gtatatcaag tagacgttaa tggcgaacgt
      121 ttcaatacaa aaccacgagg attatttaga aagaaaaaat tttcaccggt agttggtgat
      181 atagtggaat ttgaagttca aaacattaac gaaggttata ttcatcaagt gtttgagcgg
      241 gaaaatgagt tgaaaagacc acctgtaagt aatatagata cactagtaat tgtaatgagt
      301 gctgtcgagc caaatttttc aacgcaatta ttagatcgat ttttagttat tgcacattcg
      361 tatcagttaa atgcgagaat tttggtgact aaaaaagata aaacaccaat tgaaaagcag
      421 ttcgaaataa atgagttgtt gaaaatatat gaaaatattg gctatgagac tgaatttatt
      481 ggaaatgatg atgatcgaaa aaaaattgta gaagcttggc cagctggact tatagtactt
      541 agtggtcaat caggtgtcgg taagtccact ttcttaaatc attatcgtcc agaacttaat
      601 cttgagacaa atgatatatc aaaatcatta aatcgaggaa agcatactac aagacatgtc
      661 gaactattcg aacgtcaaaa cggttatatt gcagacacac ctggattcag tgctttagat
      721 tttgatcata tagataaaga tgaaataaaa gattattttc ttgaattaaa tcgatatggt
      781 gaaacatgta agtttaggaa ttgtaatcat atcaaagaac ctaattgtaa tgttaagcat
      841 caattagaga tagggaatat tgcgcaattt agatacgacc attatttaca actatttaat
      901 gaaatttcaa atagaaaggt tagatattaa atgacaaaac tatatccatc attattatct
      961 gttgattttt tggatttaca acatgaatta aaacgacttg aagaagcagg cgtcgacgga
     1021 gttcattttg atgttatgga tggtcaattt gtgcctaata tatctattgg tttaccaata
     1081 ttagatgcag taagaaaagg cacaacatta cctatagacg tacatttgat gattgaaaat
     1141 ccagaaaagt atattgcatc atttgcagaa catggtgccg atatgatttc aattcatgtc
     1201 gaatcaacgc ctcatattca tcgtgctatt caaatgatta aacatttaga taaaaaagct
     1261 ggtgtagtaa ttaatcctgg tacaccaata tcacaaattg aacctatttt agacattgtt
     1321 gattatgtac tagtgatgac agttaaccca gggtttggtg gtcaatcatt tattgatcaa
     1381 tgcgtagaaa aaatagcggg tcttaatgct attaaaatgg aacgtcaatt aaactttgat
     1441 attgaagttg atggaggcgt aaataccgat acagcgaaag tttgtgttga aaatggtgct
     1501 acaatgctag taacaggttc atttttcttt aaacaagagg attataaaaa agtcacacaa
     1561 gcattgaaag gttgagtgta tatgcatata aatttattat gttctgatcg acacttgccg
     1621 caagatattt gggccaaaag taatgaaggt aaatggggcg gcgttgatag aggtgctttg
     1681 attttattga agcatcaaat tatccctttt ttctcagtgg gagactttga ttcagtcagt
     1741 aaagaagaac gccaacttct aacagaacag ttacaaatca aaccagttca agctgaaaaa
     1801 gctgatacgg atttagcttt agcggttgat aaagctgttg cacttggatt tgatagtatt
     1861 acaatttatg gtgcaacagg cggacgatta gatcactttt ttggggcaat tcagttatta
     1921 ttgaaaaaag catattataa acatgatgtt catatagaag ttatcgatca acaaaataaa
     1981 attgaattat tgcctaaagg tcaacataca gttgaaaaag ataagagtta tccgtacatt
     2041 tcatttatac cgatgactga tgatgtagaa ctttctctag caggttttaa atataattta
     2101 gctagacaaa tgcttaatat aggttctact ttaactattt caaatgaaat tgagtctttg
     2161 caagcgaaag taactgtaca tgatgggttg attttgcaaa ttagaagtac agatttaaat
     2221 taagtatatt caaatttgtg aggattttta ataaagtgca tgagttattt aataaagtga
     2281 cgtttataga tagaatttat aacataataa tagatgaatc acgctgtaat taatgaagat
     2341 tatggcgtga tttattattg aaaggaaaat tgtaggtcct agtacattga tttatgtgcc
     2401 aaaatcgata ttgaacccat aagtccaatt tgacagagaa ccattattaa acgtattaac
     2461 caaattatat tgatagttcg aatttcacta tgaaccatta tgtattgttt ggtaacaaaa
     2521 aaagaactaa gaggttgagc tcttagttct taagtaaatg tgtcctccac ataaggtcac
     2581 attttttatg tgcaatatat ttattaaact ctagttactt taccagattt taaagcacgt
     2641 gcagaaaccc aaactttttt aggtttaccg tcaactagga ttctaacttt ttgaaggtta
     2701 gcgttccatc tacgtttagt agagtttaaa gcgtgtgaac gtctgttacc agtcgaagct
     2761 ttacgacctg ttacgaaaca ttgtttaccc atatgagtac ctcctttaat aaatataaat
     2821 acacataact acttatatac ttaattaaga tagcatagtt tcatttgaaa aacaatgaat
     2881 aattttcaca taaaagtcaa aaatactgag ttttgtgata taattgtaga ctgtgaagtt
     2941 atgtagtatg atattttaga gaaataagtg aatgaaacat ttaaaatttt tatttaatga
     3001 tactacatct attaaaagaa aacgcctata gataatcgtc atgagtgtaa attcagttta
     3061 ctcaaaatat aataggtgat ttaagcgtaa gtcattctga taagttgtac cattgatatt
     3121 ttataaaaat aatcaatgaa acttaaagcg tttatgacta cactaagtgg taaaatttta
     3181 aaagtgatta gggaaaacga ataataaact ttgaaattta ggagggacaa agatatgaca
     3241 ttagagattt caaatgatta cggtaaaatt gatatttcaa acgaagtgat tgcttcggtt
     3301 gtaggtggaa aggccgttga atgttatggt attgtaggta tggcatctag acaacaagtt
     3361 agagatggta ttgcggaaat actaggacat gaaaattatg ctaaaggcat caaagtaact
     3421 gaaaataatg gcgtagtgga tatagatatg tacattattg taagttacgg tgtgaaaata
     3481 tctgaagttg ccaataatgt acaatcaaca gtgaaatata ctttggaaaa atcacttaat
     3541 gtatcagtaa attcaatcaa tatatatgta caaggtgtac gtgtgaataa tacaggcaag
     3601 aaagcttagg aggacaactt gaaatgatta gcaaaattaa tggtaaatta tttgccgata
     3661 tgattataca aggggcacaa aatttatcta acaatgcaga tttggtagat tctttgaatg
     3721 tgtatccagt gccagatggt gatacaggaa caaatatgaa tcttactatg acttcaggtc
     3781 gcgaagaagt agagaataat ttgtcgaaaa atatcggcga attaggtaaa acattctcga
     3841 aaggtttact aatgggtgca agaggtaact ctggtgtcat cttgtcacaa ttattcagag
     3901 gattttgtaa aaatattgaa agtgaatctg aaattaattc aaaattgtta gctgaaagtt
     3961 ttcaagctgg tgttgaaacg gcatataaag ctgttatgaa accagttgaa ggtacaatac
     4021 ttacagttgc aaaagatgct gcgcaagctg caatagaaaa agcaaataat actgaagatt
     4081 gtatagaatt aatggagtac attattgtaa aagccaatga atcacttgaa aacacaccaa
     4141 acttattagc tgtacttaaa gaagttggtg ttgttgatag tggcggtaaa ggtttgttat
     4201 gcgtttacga aggattctta aaagcgctta aaggtgaaaa agttgaagcc aaagttgcaa
     4261 agatagataa agatgaattt gtacatgatg aacatgattt ccatggtgta attaatactg
     4321 aagatattat ttatggctat tgtactgaaa tgatggttcg ttttggaaag aataaaaaag
     4381 cctttgatga acaagaattc aggcaagata tgagtcaatt tggtgattct ttattagtca
     4441 ttaatgatga agaaattgtg aaagttcacg tgcataccga atacccaggt aaagtgttta
     4501 attatggtca acaatatggt gaattaatta aacttaaggt tgaaaatatg agagaacagc
     4561 accgtgaagt gattcgaaaa gaacagcaca cagctaaacc gaaaatggaa acggttgaaa
     4621 cagcaattat tactatttct atgggtgaag gtatttcaga gatatttaaa tcaatgggtg
     4681 ccacacatat cattagtggt ggacaaacga tgaatccttc tacagaagat atcgttaaag
     4741 tcattgaaca atcaaaatgt aaacgtgcaa ttattttacc gaataataaa aatatcttaa
     4801 tggcaagtga acaagcagcg agtattgttg atgcagaagc tgttgttatt ccaacgaaat
     4861 ctattcctca aggtataagc gcactattcc aatatgatgt ggacgcaaca cttgaagaaa
     4921 ataaagcgca aatggctgat tcagtaaata acgttaaatc tggttcatta acgtacgctg
     4981 ttcgtgatac gaaaattgat ggcgttgaga ttaaaaaaga cgcgtttatg ggcttgattg
     5041 aagataagat tgtaagcagc caaagtgatc aattaacaac ggttactgag ttgttaaatg
     5101 agatgttagc agaagatagt gaaatattga ctgtgattat tggtcaagat gcagagcaag
     5161 cagttacaga taacatgata aactggatcg aagagcaata tccagatgta gaagtggaag
     5221 ttcatgaagg tggacaacca atttatcaat atttcttttc agtagaataa aaatttaaaa
     5281 taaaaaacta ccaatgataa atcatcagtt ggtagttttt tattttgcta ttttagtgat
     5341 attgcgggtt aaaagtatcg ttctcgagtt gctaacaatg tcatgttcaa cttagtcatg
     5401 ataaaataaa taacatacta aatgatacgt aaaatcaaat aaaacatagg tgatttattt
     5461 tggctaaagt aaacttaata gaaagtccat attctctttt acaattaaaa ggtataggtc
     5521 ctaagaaaat agaagtattg caacaactaa atattcatac agtggaagat cttgttcttt
     5581 atttgccaac tagatatgaa gataatacag tgattgattt gaatcaagca gaagatcaat
     5641 ctaacgttac gatagaagga caagtatata cagctccagt agttgcattt tttggaagaa
     5701 ataaatcaaa attaaccgtt catttaatgg taaataatat tgctgtcaaa tgtatttttt
     5761 tcaatcaacc gtatttaaaa aagaaaatcg aattaaatca aactataact gttaaaggta
     5821 agtggaatag ggttaaacag gaaattactg gtaatagggt tttctttaat tcacaaggga
     5881 cacaaactca agaaaacgca gatgttcaat tagaaccagt ctatcgtatt aaggaaggta
     5941 ttaaacaaaa gcaaatacga gaccaaatta gacaagcgtt aaatgatgtg acaattcatg
     6001 aatggttaac tgatgaacta agagaaaaat ataaattaga gaccttggac tttactttga
     6061 acacattaca tcatcctaaa agtaaagagg atttattacg tgctcgtaga acctatgcat
     6121 ttactgaact gtttttattc gaattacgta tgcaatggct aaatagatta gaaaagtcat
     6181 ctgacgaagc aattgaaatt gattatgaca tagaccaagt taaatcattt attgatcgtt
     6241 taccttttga actaactgaa gcacagaaat ccagtgttaa tgaaattttt agagatttaa
     6301 aagcaccaat acgtatgcat cgattacttc aaggtgatgt aggttcagga aaaacagtag
     6361 ttgctgcaat ttgtatgtat gcgttaaaaa ctgctggtta tcaatcagca ttgatggtac
     6421 caactgaaat tttagcagag caacatgctg aaagtttaat ggctttattt ggagattcta
     6481 tgaacgttgc attgttaact gggtcagtaa aaggtaagaa acgaaagata cttttagaac
     6541 aacttgaaaa tggtacgatt gattgtttaa ttggaaccca tgctttgatt caagatgatg
     6601 tgattttcca taatgttggt ttagtaatta cagatgaaca acatcgattt ggtgtgaatc
     6661 aacgccagct tttaagagaa aaaggtgcaa tgacgaatgt gttatttatg acagcaacgc
     6721 cgataccaag aacactagca atatcagttt ttggtgagat ggatgtgtct tcaattaaac
     6781 aattaccaaa aggtcgtaaa cctatcatta ctacttgggc aaagcatgag caatacgata
     6841 aagttttgat gcaaatgacc tcagagttga aaaaaggtcg tcaagcatat gtcatttgcc
     6901 cgctaataga aagttctgag catctcgaag atgttcaaaa tgttgtcgca ttgtacgagt
     6961 ctttacaaca gtattatggt gtttcccgtg tagggttatt gcatggtaag ttatctgccg
     7021 atgaaaaaga tgaggtcatg caaaagttta gtaatcatga gataaatgtt ttagtttcta
     7081 ctactgttgt tgaagtaggt gttaatgtac cgaatgcaac ttttatgatg atttatgatg
     7141 cggatcgctt tggattatca actttacatc agttacgcgg tcgtgtaggt agaagtgacc
     7201 agcaaagtta ctgtgtttta attgcatccc ctaaaacaga aacaggaatt gaaagaatga
     7261 caattatgac acaaacaacg gatggatttg aattgagtga acgagactta gaaatgcgtg
     7321 gtcctggaga tttctttggt gttaaacaaa gtggattgcc agatttctta gttgccaatt
     7381 tagttgaaga ttatcgtatg ttagaagttg ctcgtgatga agcagctgaa cttattcaat
     7441 ctggcgtatt ctttgaaaat acgtatcaac atttacgtca ttttgttgaa gaaaatttat
     7501 tacatcgtag ttttgactaa ttgccatgct gatttgtcaa tttgagtgca acacttcgtt
     7561 aattgagtga tatgacactt gaactattta aatgtaaagt ggtattttaa caatttataa
     7621 attttcgact aaataatagc taaatattac agttatttgt tgagtcggtt aaatagaaag
     7681 tgttatgata tgtgaggaat gtttaagact aggtactaaa aaatgagggg tgagacgttg
     7741 aaactaaaga aagataaacg tagagaagca atcagacaac aaattgatag caatcccttc
     7801 atcacagacc atgaactaag cgacttattt caagtgagta tacaaacaat tcgtttagat
     7861 cgcacttatt taaacatacc agaattaagg aagcgtatta aattagttgc tgaaaagaat
     7921 tatgaccaaa taagttctat tgaagaacaa gaatttattg gtgatttgat tcaagtcaat
     7981 ccaaatgtta aagcgcaatc aattttagat attacatcgg attctgtttt tcataaaact
     8041 ggaattgcgc gtggtcatgt gctgtttgct caggcaaatt cgttatgtgt tgcgctaatt
     8101 aagcaaccaa cagttttaac tcatgagagt agcattcaat ttattgaaaa agtaaaatta
     8161 aatgatacgg taagagcaga agcacgagtt gtaaatcaaa ctgcaaaaca ttattacgtc
     8221 gaagtaaagt catatgttaa acatacatta gttttcaaag gaaattttaa aatgttttat
     8281 gataagcgag gataaaatta tggttaaatt agcaattgat atgatgggtg gcgacaatgc
     8341 gcctgatatc gtattagaag ccgtacaaaa ggctgttgaa gactttaaag atctagaaat
     8401 tatacttttc ggtgacgaaa aaaagtataa tctgaaccat gaacgaatcg aatttagaca
     8461 ttgttctgaa aagattgaaa tggaagatga gcctgttaga gcgattaaac gtaaaaaaga
     8521 tagctcaatg gtaaaaatgg ctgaagctgt gaaatctggt gaagcagatg gatgtgtgtc
     8581 agcaggtaat actggtgctt taatgtcagc tggtttattc attgttggac gtattaaagg
     8641 tgtagctaga ccggctttag tagtaacatt gccaacgatt gatggaaaag gttttgtctt
     8701 tttagacgtt ggtgcaaatg ctgatgctaa acctgaacac ttattacagt atgcgcaact
     8761 aggggatatt tatgctcaaa aaattagagg tattgataat ccgaaaatct cattattaaa
     8821 tataggaacc gagccagcta aaggtaatag tttaacgaaa aaatcatatg agttattaaa
     8881 tcatgatcat tcattgaatt ttgttgggaa tattgaagcg aagacattaa tggatggcga
     8941 tacagatgtt gtagttaccg atggctatac tgggaacatg gtccttaaaa atttagaagg
     9001 tactgcaaaa tcaatcggta aaatgttaaa agatacgatt atgagtagta ctaaaaataa
     9061 attagcaggt gcaatattga agaaagattt agctgaattc gctaaaaaga tggattactc
     9121 agaatacggt ggttccgtat tattaggatt ggaaggtact gtagttaaag cacacggtag
     9181 ttcaaatgct aaagcttttt attctgcaat tagacaagcg aaaatcgcag gagaacaaaa
     9241 tattgtacaa acaatgaaag agactgtagg tgaatcaaat gagtaaaaca gcaattattt
     9301 ttccgggaca aggtgcccaa aaagttggta tggcgcaaga tttgtttaac aacaatgatc
     9361 aagcaactga aattttaact tcagcagcga acacattaga ctttgatatt ttagagacaa
     9421 tgtttactga tgaagaaggt aaattgggtg aaactgaaaa cacacaacca gctttattga
     9481 cgcatagttc ggcattatta gcagcgctaa aaaatttgaa tcctgatttt actatggggc
     9541 atagtttagg tgaatattca agtttagttg cagctgacgt attatcattt gaagatgcag
     9601 ttaaaattgt tagaaaacgt ggtcaattaa tggcgcaagc atttcctact ggtgtaggaa
     9661 gcatggctgc agtattggga ttagattttg ataaagtcga tgaaatttgt aagtcattat
     9721 catctgatga caaaataatt gaaccagcaa acattaattg cccaggtcaa attgttgttt
     9781 caggtcacaa agctttaatt gatgagctag tagaaaaagg taaatcatta ggtgcaaaac
     9841 gtgtcatgcc tttagcagta tctggaccat tccattcatc gctaatgaaa gtgattgaag
     9901 aagatttttc aagttacatt aatcaatttg aatggcgtga tgctaagttt cctgtagttc
     9961 aaaatgtaaa tgcgcaaggt gaaactgaca aagaagtaat taaatctaat atggtcaagc
    10021 aattatattc accagtacaa ttcattaact caacagaatg gctaatagac caaggtgttg
    10081 atcattttat tgaaattggt cctggaaaag ttttatctgg cttaattaaa aaaataaata
    10141 gagatgttaa gttaacatca attcaaactt tagaagatgt gaaaggatgg aatgaaaatg
    10201 actaagagtg ctttagtaac aggtgcatca agaggaattg gacgtagtat tgcgttacaa
    10261 ttagcagaag aaggatataa tgtagcagta aactatgcag gcagcaaaga gaaagctgaa
    10321 gcagtagtcg aagaaatcaa agctaaaggt gttgacagtt ttgcgattca agcaaatgtt
    10381 gccgatgctg atgaagttaa agcaatgatt aaagaagtag ttagccaatt tggttcttta
    10441 gatgttttag taaataatgc aggtattact cgcgataatt tattaatgcg tatgaaagaa
    10501 caagagtggg atgatgttat tgacacaaac ttaaaaggtg tatttaactg tatccaaaaa
    10561 gcaacaccac aaatgttaag acaacgtagt ggtgctatca tcaatttatc aagtgttgtt
    10621 ggagcagtag gtaatccggg acaagcaaac tatgttgcaa caaaagcagg tgttattggt
    10681 ttaactaaat ctgcggcgcg tgaattagca tctcgtggta tcactgtaaa tgcagttgca
    10741 cctggtttta ttgtttctga tatgacagat gctttaagtg atgagcttaa agaacaaatg
    10801 ttgactcaaa ttccgttagc acgttttggt caagacacag atattgctaa tacagtagcg
    10861 ttcttagcat cagacaaagc aaaatatatt acaggtcaaa caatccatgt aaatggtgga
    10921 atgtacatgt aatatatttg agctaaagct cattgacgca gtggttgact ggtcatccaa
    10981 tggagaattg tctgacctag tcaactttgc gggggaaatt ctaagcaacc tagataaggt
    11041 tccagaattt ctccctaaga aacactaatc aataaattga taagtgtttc taaaatttct
    11101 acttgttttt tagaatttaa aatgggaaaa tatagtagtc tatgtatagg catttttaaa
    11161 ggaggtgaat cgacgtggaa aatttcgata aagtaaaaga tatcatcgtt gaccgtttag
    11221 gtgtagacgc tgataaagta actgaagatg catctttcaa agatgattta ggcgctgact
    11281 cacttgatat cgctgaatta gtaatggaat tagaagacga gtttggtact gaaattcctg
    11341 atgaagaagc tgaaaaaatc aacactgttg gtgatgctgt taaatttatt aacagtcttg
    11401 aaaaataata aatcttacat ctgggtcgtc agtattgtcg actcagtttt tttctttaat
    11461 tatcaatagt tttaacgtaa aattaaagat gattcaagag caacacataa aggagataaa
    11521 ataatgtcta aacaaaagaa aagtgagata gttaatcgtt ttagaaagcg ctttgatact
    11581 aaaatgacag agttaggctt tacttatcaa aatattgatt tataccaaca agcattttcg
    11641 cattcgagtt ttattaatga ttttaatatg aatcgtttag accataatga gcgtttagag
    11701 tttttgggtg atgcggtatt agaattgacg gtttcacgat atttatttga taaacatccc
    11761 aacttgccag aagggaattt aacaaaaatg cgtgccacta ttgtatgtga gccctcactt
    11821 gtaatatttg cgaataaaat tggattgaac gaaatgattt tacttggtaa aggtgaagag
    11881 aaaacagggg gacgtacaag accatcatta atatcagatg cattcgaagc atttattggg
    11941 gcattgtatt tggatcaagg actagatata gtttggaaat ttgctgagaa agtcattttc
    12001 ccacatgtag aacaaaatga gttattaggc gtggtagatt ttaaaacaca attccaagaa
    12061 tatgtgcacc agcaaaataa aggtgatgta acctataatt taataaaaga agagggaccg
    12121 gcacatcatc gtctattcac ttcagaagtt attctgcaag gggaagcaat agctgaaggt
    12181 aaagggaaaa cgaaaaaaga atcagaacaa cgtgctgctg aaagtgccta taagcaatta
    12241 aaacaaatta aatagaaatt tatatgatac gacatatagg tgttcgattt aggatttaag
    12301 gaagaatttt taatatttta tggaacaact tcgaaattta atatacaaat actaaaatat
    12361 cgtatacatg tgttctttaa aattgtgata aggagtttag gatggtttat ttaaaatcaa
    12421 tagatgccat tggatttaag tcttttgcag atcaaaccaa tgttcaattc gataaaggtg
    12481 taactgcaat tgttggtcca aatggaagcg gtaaaagtaa tattacagat gctattaaat
    12541 gggtgttggg tgaacaatcg gctaaatcat tacgtggctc aaaaatggaa gatattatct
    12601 tctcaggtgc agaacatcgc aaagctcaaa attatgctga agtacagtta agattagata
    12661 atcattctaa aaagctcagt gttgatgaaa acgaagttat tgtaacaaga agattgtatc
    12721 gaagtggtga aagtgagtac tacataaata atgaccgtgc aagattaaaa gatattgccg
    12781 atttattttt agattctgga ttgggaaaag aagcgtatag cattatctcg caaggtagag
    12841 ttgatgaaat actaaatgct aaaccaattg atagacgtca aattattgaa gaatcggctg
    12901 gtgtacttaa atataaaaaa cgtaaggctg aatcattaaa taaacttgac caaacagaag
    12961 ataatttaac gagagtagaa gacattttat atgatttgga aggtcgcgta gaacctctaa
    13021 aagaggaggc agctatagct aaagaatata agacactttc acatcaaatg aaacatagtg
    13081 acattgtagt tacagtgcac gatattgatc aatatacaaa tgacaataga caattagatc
    13141 aacgtttaaa tgatttacaa ggccaacaag caaataaaga agctgacaag caacgtttaa
    13201 gccaacaaat tcaacaatat aaaggtaaac gtcatcaact tgataatgat gttgaatcgc
    13261 ttaattatca attagtaaaa gctacggaag cctttgaaaa atatacggga caattaaatg
    13321 ttttggaaga acgtaagaaa aatcaatctg aaacaaatgc acgatatgaa gaagaacaag
    13381 aaaatttaat ggagctttta gaaaatatat caaatgagat ttctgaagct caagatactt
    13441 ataagtctct gaaaagtaaa caaaaagaac tcaatgctgt cattcgtgaa cttgaagaac
    13501 aactatatgt ttcagacgaa gcacatgatg aaaaattgga agaaattaaa aacgaatact
    13561 atacattaat gtcagagcaa tcagatgtta acaatgatat tcgtttttta aagcatacta
    13621 tagaagagaa tgaggctaaa aaatcaagac tagattctcg attagttgaa gtttttgagc
    13681 aattgaaaga tattcagggt caaataaaaa cgacaaaaaa agaatatcaa cagaccaaca
    13741 aagaactttc tgctgtagat aaagaaatta aaaatataga aaaagacctc actgatacaa
    13801 aaaaagcaca aaatgaatac gaagagaaat tgtatcaagc atatcgatat accgaaaaaa
    13861 tgaaaacacg tattgatagt ttggcaacgc aagaggaaga atatacttat tttttcaatg
    13921 gcgtcaaaca tattttgaaa gctaaaaata aagaattaaa gggtatttat ggtgcagttg
    13981 cggaaattat tgatgtgcca tctaaattaa ctcaggcaat tgaaacagca ttaggtgctt
    14041 cattacaaca tgtcattgta gattcagaaa aagatggacg ccaggctatt caatttttaa
    14101 aagaacgtaa tttaggtcgt gcgacgtttt taccattaaa tgttatacag agtagagtgg
    14161 tagcgactga tattaaatct attgctaaag aggcaaacgg atttattagt atcgcttcgg
    14221 aagcagttaa agtagcacca gaatatcaaa atattatcgg gaatttatta ggtaatacga
    14281 ttatcgttga tcatttaaag catgcaaatg aattggcacg tgcgattaaa tatcgaactc
    14341 gtattgttac tttggaaggt gatattgtaa atcctggtgg ttctatgact ggtggtggcg
    14401 ctcgtaagtc aaaaagtatt ctgtctcaaa aagacgagtt gacaacaatg agacaccaat
    14461 tagaagatta cttgcgtcaa acagaatcat ttgaacaaca atttaaagag ttgaagataa
    14521 aaagtgatca attaagtgaa ctgtattttg aaaaaagtca aaagcataat acacttaaag
    14581 agcaagtgca tcattttgaa atggagctcg atagattaac tacacaagaa acacaaataa
    14641 aaaatgatca tgaagaattc gaatttgaaa aaaatgatgg ttatacgagt gacaaaagtc
    14701 gacaaacttt gagtgaaaaa gaaacttatc tagaaagtat taaagcatct ttaaaacgac
    14761 tagaagatga aattgaacgc tacacaaaac tttctaaaga aggtaaggaa agcgttacta
    14821 aaacacaaca aaccttacat cagaaacaat ctgatcttgc tgtggttaaa gagcgtatta
    14881 aaacacaaca acagacaata gatcgattaa ataatcaaaa tcaacaaact aaacatcaat
    14941 taaaagatgt taaagaaaaa attgcattct ttaattcgga tgaagtgatg ggcgaacaag
    15001 cttttcaaaa tattaaagat caaattaatg gtcaacaaga aacgagaaca cgcttatcag
    15061 atgaattaga taaattgaaa caacaacgta ttgagttgaa tgaacaaatc gatgcgcaag
    15121 aagctaaact acaagtttgt caccaagata ttttagctat cgaaaatcac taccaagata
    15181 ttaaagctga acaatcaaag ctagatgtat taattcatca tgcgatagat catttaaatg
    15241 atgaatatca attgactgtt gaacgtgcga aatctgaata tacgagtgat gaatcgattg
    15301 acgcattacg taaaaaagtt aagttaatga agatgtcgat tgatgaacta ggtcctgtaa
    15361 acttaaatgc aattgaacaa tttgaagagt taaatgaacg ttatacattt ttaagtgaac
    15421 aacgtacaga tcttcgtaaa gctaaagaaa cattagagca aattataagt gaaatggatc
    15481 aagaggttac tgaaagattt aaagaaactt tccatgctat tcaaggacat tttacagctg
    15541 tgttcaaaca attgtttggt ggaggcgatg cagaattgca attaactgaa gccgattatt
    15601 taacagctgg tattgatatt gtggtacaac caccgggtaa aaagttgcaa catttatcgt
    15661 tactgagtgg tggtgagcgt gcattaactg ctattgcttt actatttgca attttaaaag
    15721 taagatctgc accttttgtt atattagatg aggttgaagc tgcactagat gaagcaaatg
    15781 ttattagata cgcaaaatat ttaaatgagt tatcagacga aacacaattc attgttatta
    15841 cacaccgtaa aggaacaatg gaatttgcag ataggttata cggtgtaaca atgcaagaat
    15901 caggtgttac taaacttgtg agtgtgaatt taaatacaat agatgatgtg ttgaaggagg
    15961 agcaataatg agctttttta aacgcttaaa agataagttt gcaacaaata aagaaaatga
    16021 agaagttaaa tccttaacag aagaacaagg tcaagacaaa ttagaagata cacattctga
    16081 aggttcaacg caggacgcaa atgatttagc agaaaatgct gaagtgaaaa agaagccacg
    16141 caagttgagt gaagcggatt ttgatgacga tggcttaata tcaattgaag attttgaaga
    16201 aattgaagct caaaaaatgg gtgctaaatt taaagcagga ctcgaaaaat ctcgtcaaaa
    16261 tttccaagaa caattaaata atttgatagc gagatatcgt aaagtagatg aagacttttt
    16321 tgaagcttta gaagaaatgt taatcactgc agacgtcggt tttaatacag tgatgacgtt
    16381 aactgaagaa ttacgtatgg aagcacaacg acgtaatatt caagatactg aagatttgcg
    16441 tgaagtcatt gttgaaaaaa tcgtagagat ttaccatcaa gaagatgata attcagaagc
    16501 tatgaactta gaagatggtc gtttaaatgt cattttaatg gttggtgtga atggtgttgg
    16561 taaaacaaca acaattggaa aattagctta ccgatataaa atggaaggta aaaaagtaat
    16621 gttagctgcg ggcgatactt ttagagcggg tgctattgat caattgaaag tttggggcga
    16681 acgtgttggt gtagacgtaa ttagccaaag tgaaggttct gatccagctg ctgttatgta
    16741 tgatgcgatt aatgccgcta aaaacaaagg tgttgatatt ttaatctgtg ataccgctgg
    16801 acgtttacaa aataaaacaa atctaatgca agaattagaa aaagttaagc gtgtaattaa
    16861 tcgagcagtg ccagatgcgc ctcatgaagc attactatgt ttagatgcta caactggtca
    16921 gaatgcgttg tcacaagcta gaaactttaa agaagtaaca aatgttacag gtattgtatt
    16981 aacgaaatta gatggtacag ccaaaggtgg tatcgtatta gccattcgta atgaattgca
    17041 catcccagtt aaatatgtag gtttaggtga gcaattagat gacttacaac catttaaccc
    17101 tgaaagttat gtctacggct tattcgctga tatgattgaa caaaatgaag aaataacaac
    17161 agttgaaaat gatcaaattg taacagaaga aaaggacgat aatcatgggt caaaatgatt
    17221 tagttaaaac gttacgaatg aattatttgt ttgattttta tcaatcctta ttgacgaata
    17281 aacaacgtaa ttatttggaa ttattttatc ttgaagatta ttctttaagt gaaatcgcag
    17341 atacttttaa tgtgagtaga caagcagttt atgataatat aagaagaact ggcgatttag
    17401 ttgaagatta tgaaaagaaa ttggaattat accagaaatt tgagcaacgc cgagaaatat
    17461 atgatgaaat gaaacaacat ttaagtaatc cagaacaaat acaacgttat attcaacaat
    17521 tagaagactt agaatagata taaattgagg gaggacatcg atatggcatt tgaagggtta
    17581 tcagaacgct tgcaagcgac gatgcaaaaa atgcgtggta agggtaaact tactgaagct
    17641 gatataaaga taatgatgcg tgaagtaaga ttagcgttac ttgaggctga cgtaaacttt
    17701 aaagtggtaa aagaatttat taaaacagta tcagaacgcg cattaggttc cgatgtaatg
    17761 caatcattaa caccagggca acaagttatt aaaatagttc aagatgaatt aacgcagttg
    17821 atgggtggag aaaatacgtc gattaatatg tcaaataaac cacctactgt tgttatgatg
    17881 gttggtttac aaggtgctgg taaaacaaca actgcaggta aattagcatt attgatgcgt
    17941 aaaaaataca acaaaaaacc tatgttagtt gcagcagata tttatcgtcc agcagcgata
    18001 aatcaattac aaacagtagg gaaacaaatt gatattcctg tatacagtga aggagatcaa
    18061 gtaaagccac aacaaattgt aactaatgca ttaaaacatg ctaaagaaga acatttagac
    18121 tttgtaatca ttgatacagc aggtcgatta cacatcgatg aagcattgat gaacgaatta
    18181 aaagaagtaa aagacattgc taaaccaaac gaaattatgt tagttgtcga ttcaatgacg
    18241 ggtcaagatg ctgtcaatgt tgcagaatct tttgacgatc aacttgatgt cacaggtgtt
    18301 accttaacta aattagatgg tgatacacgt ggtggtgcag ctttatctat tcgttcggtg
    18361 acacaaaaac caattaaatt tgttggtatg agtgaaaagt tagatggttt agagctattc
    18421 catcctgaac gtatggcatc acgtatttta ggtatgggtg atgtgttaag tttaattgaa
    18481 aaagcgcaac aagatgtgga tcaagaaaaa gcaaaagatt tagagaaaaa gatgcgtgag
    18541 tcatcgttta ctttagatga ttttttagaa caacttgatc aggtgaaaaa tctaggacca
    18601 ctggatgata ttatgaaaat gattccaggt atgaataaaa tgaaagggct agataagctt
    18661 aatatgagtg aaaagcaaat tgatcatatt aaagcgatta tccagtcaat gacgccggct
    18721 gaaagaaaca atccagacac attgaatgta tcacgtaaaa agcgtattgc taaagggtct
    18781 ggtcgttcat tacaagaagt caatcgtttg atgaaacaat ttaacgatat gaagaaaatg
    18841 atgaaacagt tcactggtgg cggtaaaggt aaaaaaggta aacgcaatca aatgcaaaat
    18901 atgttaaaag gtatgaattt accgttttaa tgtttaatat aacacgctga tttttgttgc
    18961 aatgagatgt gcatcccgaa agttatacta aagaatataa ctttatgggg tgcatatttt
//
LOCUS       DAKTIP010000030        18728 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAKTIP010000030 DAKTIP010000000
VERSION     DAKTIP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.030"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..282)
                     /locus_tag="O0305_000833"
     CDS             complement(<1..282)
                     /locus_tag="O0305_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCY0273989.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAID"
     gene            544..729
                     /locus_tag="O0305_000834"
     CDS             544..729
                     /locus_tag="O0305_000834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273990.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
     gene            complement(952..1530)
                     /locus_tag="O0305_000835"
     CDS             complement(952..1530)
                     /locus_tag="O0305_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0273991.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0305_000836"
     CDS             complement(1794..2174)
                     /gene="blaI"
                     /locus_tag="O0305_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0273992.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(2164..3920)
                     /gene="blaR1"
                     /locus_tag="O0305_000837"
                     /pseudo
     CDS             complement(2164..3920)
                     /gene="blaR1"
                     /locus_tag="O0305_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            4027..4872
                     /gene="blaZ"
                     /locus_tag="O0305_000838"
     CDS             4027..4872
                     /gene="blaZ"
                     /locus_tag="O0305_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0273993.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            complement(5398..5817)
                     /locus_tag="O0305_000839"
     CDS             complement(5398..5817)
                     /locus_tag="O0305_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0273994.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            5941..6912
                     /locus_tag="O0305_000840"
     CDS             5941..6912
                     /locus_tag="O0305_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0273995.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            complement(7084..7176)
                     /locus_tag="O0305_000841"
     CDS             complement(7084..7176)
                     /locus_tag="O0305_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0273996.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(7333..7680)
                     /locus_tag="O0305_000842"
     CDS             complement(7333..7680)
                     /locus_tag="O0305_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0273997.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(7699..8316)
                     /gene="cadD"
                     /locus_tag="O0305_000843"
     CDS             complement(7699..8316)
                     /gene="cadD"
                     /locus_tag="O0305_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCY0273998.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(9432..9626)
                     /locus_tag="O0305_000844"
     CDS             complement(9432..9626)
                     /locus_tag="O0305_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0273999.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYC
                     LLPLIVNLSIYGFYVFVKRK"
     gene            9736..10428
                     /locus_tag="O0305_000845"
     CDS             9736..10428
                     /locus_tag="O0305_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274000.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKS
                     KNIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEAN
                     DKNAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKN
                     YVEYKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAV
                     CGTAFAAGCSVG"
     gene            complement(10596..10745)
                     /locus_tag="O0305_000846"
     CDS             complement(10596..10745)
                     /locus_tag="O0305_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274001.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIV
                     FYEDK"
     gene            complement(11292..11608)
                     /locus_tag="O0305_000847"
                     /pseudo
     CDS             complement(11292..11608)
                     /locus_tag="O0305_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(11601..12584)
                     /locus_tag="O0305_000848"
     CDS             complement(11601..12584)
                     /locus_tag="O0305_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0274002.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENRTSRSSKIERPE
                     VRKSNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQ
                     EFPEQLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLK
                     KAIHDRGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
     gene            13010..13753
                     /locus_tag="O0305_000849"
     CDS             13010..13753
                     /locus_tag="O0305_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0274003.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            complement(13980..14165)
                     /locus_tag="O0305_000850"
     CDS             complement(13980..14165)
                     /locus_tag="O0305_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0274004.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(14170..14508)
                     /locus_tag="O0305_000851"
     CDS             complement(14170..14508)
                     /locus_tag="O0305_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274005.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            complement(14754..15362)
                     /locus_tag="O0305_000852"
     CDS             complement(14754..15362)
                     /locus_tag="O0305_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0274006.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            complement(15848..16747)
                     /locus_tag="O0305_000853"
     CDS             complement(15848..16747)
                     /locus_tag="O0305_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0274007.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            complement(16846..18312)
                     /gene="msr(A)"
                     /locus_tag="O0305_000854"
     CDS             complement(16846..18312)
                     /gene="msr(A)"
                     /locus_tag="O0305_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0274008.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
BASE COUNT         6470 a         2836 c         2547 g         6875 t
ORIGIN      
        1 atcaatggca cgatataaat agctccattt tccttttatt ttgatgtacg tctcatcaat
       61 acgccatttg taataagctt ttttatgctt tttcttccaa atttgatata aaattggggc
      121 atattcttga acccaacggt agaccgttga atgatgaacg tttacaccac gttcccttaa
      181 tatttcagat atatcacgat aactcaatgc atatcttaga tagtagccaa cagctacagt
      241 gataacatcc ttgttaaatt gtttatatct gaaatagttc atacagaaga ctcctttttg
      301 ttaaaattat actataaatt caactttgca acagaacctc atttactata tgggcatctc
      361 tttcattatt aatacaaagc tttgtatctc cgatattagg caaaggtgta aacgaattag
      421 gtgatactat aggattcaac attatattaa tcgtttcttt aataatgatt atcttcttat
      481 tttttataaa tcgtagtaaa aatatctcct atgattatat aaaagttcaa agaggtgaag
      541 ccaatgaata aattagaaaa atggtctttg gtatttatta tttttacatt tctctcttca
      601 ttagcacttt tatatttcaa atataatttt agttcggtta tgccagtcct aagtctttct
      661 attttaacta tactaatatt gttgacgagt attattactg tagtttcagt taatgatgaa
      721 gatacttaaa acgttcatta gtgaaacagg tattatatta aaaaagcgta cgaatttgta
      781 cgcttttttc ttgtgttatt attaaaatct cataacagga aaatattatg agattttaga
      841 cgctgtcaga acgtctcttt tacatcgagt gacattgaat caaagtcttt gtatgttaat
      901 gatatataaa attatttagt agattcatta tagtaagcta gattagttgc tctacttgtt
      961 tttattctct tttgcctcta atttattgag ataacgataa atagttgtac gtgaaacttg
     1021 ccactgctct gcaattgttt taattggcgt accattatca taaagtgtct taagtaagtt
     1081 taaatcttgt ttatttaatt tttcaggtct acctccatat cggcctctag cacgtgctgc
     1141 aattcgaccg gctgatgatc gctctaaaat taaattacgt tcaaactctg caaatgctgc
     1201 gaataaatga aataataatt gtcctgtaga acttgattta tccatcgtta tattttcttc
     1261 taaactatga aaactcacac ctcgttcgtt aagttcatta actaacgtga ttaaatcctc
     1321 catgttccgt cctagtctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     1381 ttcaattgct ttatctaaac caggtcgctt actttttgaa ccactcatat ggtcattaaa
     1441 tatcttttca caaccatatg tatttaatcg atcctcctgt aaattcaaat tttgtaatcc
     1501 agttgaaact cttgcataac ctattttcaa aatacagacc tcctatatac acctttagtg
     1561 taccataact tgattacgag gtcttttgtg aacatagatt ttttgttggg tttttgaaca
     1621 cataattcta tgtaatactt acttatttag acaaaaaaca agagtgtaca gaaaatggtt
     1681 attttctgga cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt
     1741 aataagagtc aagtatggtt tacatatgta ttatgtttga aacatacctc taattacttt
     1801 ttactaatat catttaaaat gtctcgcaat tcttcaattt ctttgttatt taattcttca
     1861 tttttcgcaa aattcagcac taaacttttc atatcccctc catacagttt attaagaaag
     1921 gttttagcag ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata
     1981 ttttctgatt tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta
     2041 atcgttttat cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat
     2101 actgattttt tatcccatat tatattcata acatcccatt cagccataga tatttcaact
     2161 tgcttattgg tcatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc
     2221 atttttccca gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt
     2281 tgtaattacg taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc
     2341 tgttttccca tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg
     2401 attctttgct tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac
     2461 ttgttcaaga ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa
     2521 attttcatta ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg
     2581 attactaata cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg
     2641 ttccttattc caagactcga aaagataatg tttatgattc catgacatac gtgaattttt
     2701 atcacttata atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc
     2761 aggagaatat ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat
     2821 gctgtacata acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg
     2881 gtaatcgctt tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa
     2941 ctggctttga attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt
     3001 tgactgcttt ttcagatcgg cttcttttat attaattaat ctacttttga gtaatgattt
     3061 tttaccatta aatgaatggc ttaatatatt tttgttggaa aatggggttt ttaatacgga
     3121 gtccataata gattccgcat aagttttaaa ttcatttttg ttaatattat taaggacaaa
     3181 tctatcggct tctacttcat tgtcatgaat tatctttctt tttacaatat gtactaatgg
     3241 attgtaactc ataataatag agaagatatt aaagataatt aaatgtaaag tatctctatt
     3301 tttagcatga gcatattcat gcagtattat atattttagt cttttatcaa ttacactttt
     3361 aaaatatgaa ctaggaatca aaataatata tttcccatac caaaaagtta ttggagattg
     3421 aatagcctct gcttttcgaa tcacaatatt tttttatatt gatggttgaa aagtatcgta
     3481 tctattttat ttttttcatt ttcgtttaga taaagtgact gtttctttaa atatttaaga
     3541 tataataagg ctttcaaaaa tttaaaactt aaaataataa ctaaaactat ccaaataact
     3601 gtgcagatat tatcaattga atcccaatta aacttatgga tatctgttgt gaactcttga
     3661 ataggtttgg tggtatttat gttatggttc aaatcgtgtg acttactttc aactgtgggc
     3721 gcttgattat tcacattatt aaattttata aaagagaatt taattgggat aaaaggaatt
     3781 aatccagcaa gcatagttag ataccaaact ttataattta aactataatt aaaatagcgt
     3841 tttaatatgt acctaaaaaa tactaacaat agaaatataa aacaaaagct tactacgctc
     3901 attattagta atttagccat tttgacacct tctttcaaat atttataata aacaattgac
     3961 atcaatatta caattgtaat attattgatt tataaaaatt acaactgtaa tatcggaggg
     4021 tttattttga aaaagttaat atttttaatt gtaattgctt tagttttaag tgcatgtaat
     4081 tcaaacagtt cacatgccaa agagttaaat gatttagaaa aaaaatataa tgctcatatt
     4141 ggtgtttatg ctttaaatac taaaagtggt aaggaagtaa aatttaattc agataagaga
     4201 tttgcctatg cttcgacttc aaaagcgata aatagtgcta ttttgttaga acaagtacct
     4261 tataataagt taaataaaaa agtacatatt aacaaagatg atatagttgc ttattctcct
     4321 attttagaaa aatatgtagg aaaagatatc actttaaaag aacttattga ggcttcaatg
     4381 acatatagtg ataatacagc aaacaataaa attataaaag aaatcggtgg aatcaaaaaa
     4441 gttaaacaac gtctaaaaga actaggagat aaagtaacaa atccagttag atatgagata
     4501 gaattaaatt actattcacc aaagagcaaa aaagatactt caacgcctgc tgctttcggc
     4561 aagactttaa ataaacttat cgcaaatgga aaattaagca aaaaaaataa aaatttctta
     4621 cttgatttaa tgtttaataa taaaaacgga gacactttaa ttaaagatgg tgttccaaaa
     4681 gactataagg ttgctgataa aagtggtcaa gcaataacat atgcttctag aaatgatgtt
     4741 gcttttgttt atcctaaggg ccaatctgaa cctattgttt tagtcatttt tacgaataaa
     4801 gacaataaaa gtgataagcc aaatgataag ttgataagtg aaaccgccaa gagtgtaatg
     4861 aaggaatttt aatattctaa atgcataata aatactgata acatcttata gtttgtatta
     4921 tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt
     4981 attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa
     5041 aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt
     5101 atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc
     5161 aagcaaagtg acaatagcat tttacaagta ttttttattg ttcgattagg gtaaacctaa
     5221 ataatacata aattatatta caatatgttt attatagagg ctaaagtatc ttagaaattt
     5281 ggatagtagg tttttcggga ataaaagtat agttggcttt aatatataat taagctaagc
     5341 tatcatttat atttactcga ttataattcg cacttcaact tttttataac gtaaaaatta
     5401 aaacgtagtt ttatttatct ttagtaatat ctttttaaac actacagttt cttcattagt
     5461 taaattagcg aagtaatgat tattaagttg ataggctttc ttttctaatg ttggccataa
     5521 gtcttttgct ttatttgtac tgtttattag cacagttctt ctattttcat tatcagtttg
     5581 acgtgttatt agatcctttt tggacatctt atctaatatt ccagatattg ttgaaggttc
     5641 taaatttaaa gtattaccaa tcgctttagg tgtattcaaa ttactattaa tcaagcaatt
     5701 caaaacacca tattctgctg gagtaatatt atagggactt aataaacttt taaaatgttt
     5761 aaaaactcta ttctgagaag tactcaacaa ataatttata caattatcta attccacaac
     5821 taaaacctct cttaaatcat tagtattgac tcgcttatca tatcatgata agttttgtag
     5881 gaaaatagtt ttgcaacaaa aacatacttc tacaaaacaa ttgtgaaaga aggctaataa
     5941 atgttcaatg cggtagtagt gagaaaaagt gcatcaaatg ttagttataa agtagaaaaa
     6001 gttgatgaat ctcatttatc gaagggtgat gtattagtaa aagtggtata ctcttcaata
     6061 aattataagg atatgctagc tttacaatat aaaggcggtg taattagaga ttatcctatg
     6121 attcctggta tagattttgc aggtattgtt gaatcaagtt cgaatgataa gtttaaagaa
     6181 ggcgataatg tattagttac tggttatgat gttggcgtga cacatacagg tggattttca
     6241 gaatatgcac aagtgcctgg agagtggatt gttcctattc cccaagaaat gtctattaaa
     6301 gagtctatgg tatatggcac agccggtttt acggcagcat tatcaatttt tgaactagaa
     6361 aaatatggaa tgaatatcaa atcacaacct gaaattttag tgtctggggc tacaggtggt
     6421 gtgggcagtg ttgctataca aattttacat aaaattggat atgaaaatat cactgctatc
     6481 gtaagaaaag aaaagcaaat agaaatcgca aaaaaattag gtgcaaaaca agtaataata
     6541 attgaagatg ataataaacc gcttaaaaaa ggtatttttg attatttctt agatacagtg
     6601 gggagtaatg taacattata cggtttaaaa cgtctaaatt atcaaggtgt tgctactgta
     6661 tgtggaaatg tagcaggaaa ttcacttaac gctaatatat taccgtttat tcttagaggt
     6721 attaaattaa ttggcattga ttctgtatat gttgatcata atatccgtga agatatatgg
     6781 agtaagttag caaatgaatg gaatatagga aattcacttc tatacaatga aattggattt
     6841 gaagaattct ataaaacgat agatcagctt ttaaaaggac aacatttagg aagaactatt
     6901 ttaaaagtat aagtgtgagt attcattaat acatttacta ttaattcaat cattttgcat
     6961 ttctctacta accagtgtta taatttatat aacgaaaatg attaacaatt aatgcaccaa
     7021 tccccacact attgcagtag tgtggggatt ttttcgatgc tggcttataa agtcgcctat
     7081 taattatttg ttgcgtttac taagccaata agtataatac gcaacagcac agccactgat
     7141 gactggtgct atgatgtgaa cgaaaatgat tagcaaagta atgcacctcc tctcttcgtc
     7201 aattgacgcc tgagagatag gcgactctat tattatacca tctgtgaata tacaagcata
     7261 tctacttccg ataagtcgtc ttatgttata taactttgtt ttttatagta tcaagacaag
     7321 aagaaactcg tttcaaaaac cctcatgatt tttagagaaa aactctctta tttcatcgtc
     7381 tttaataaaa taatatgcca tctttccatc tttataaaaa tcaagtacct catttttata
     7441 taaaagcctt aaatgatgtg aagttgaagc aacactcatt ttcaatatca aagaaatatc
     7501 acaaacacac aactcatctt ctttaatcaa agataatatg attttcaatt tcttctcatc
     7561 acaaattttt tctaaaatgt taagtaattt cttagattta tcatcttcta aaaaacttaa
     7621 agcattgtta actttatcct catgtacaca gatcacatca caagtatttt cataactcat
     7681 ataaaatcac accctaaatt aaaatataaa tcctaaaatt gtttgaatag tgtcattttc
     7741 aataataata aataaaccta aagctatata aataatagcc ataatccaac gactaaattt
     7801 ctcaacaatt tctccaattc ctggaatatt agctaatttt tgtgcagtaa acaccaagaa
     7861 gaaaattaaa attaaaaaga caaacagagt aagtaataaa ttagtaacac ttaatgtcac
     7921 aaaatacgga acaaataaac caatattatc ggcgccacaa cttgctatcg taacaattgc
     7981 aaccgtacca actaatttag acaatccttt ttcattcaat tcttttttag ctctcttttc
     8041 tccttcacaa tcatcataaa tagccacttt aattccaaga taaattggta ttaaacccaa
     8101 taaacctaat atccactttt ctggaacata atttaataca aaagctaaaa acaaactaac
     8161 taatattaaa ataatagacc ctaaatattg accaacataa atatctctat attcttttct
     8221 agtctttgct ctagcaaaaa atattaataa aattactaat aaatcaactg ctgtagcaat
     8281 ataaagaaca gcagcagcaa caaccgtttg aatcataata cacctcattc aaaaatattt
     8341 ttgaatgtat tataacttat atttttaaat aatcataggt gctttattcc aattgcttta
     8401 ttgacgttga gcctcggaac ccttaacaat cccaaaactt gtcgaatggt cggcttaata
     8461 gctcacgcta tgccgacatt cgtctgcaag tttagttaag ggttcttctc aacatcaata
     8521 aattttttcg gcataaatgc gtgtctatgc gtagacatgg agacacttca gaattatgga
     8581 gtgattacat gagtttaact atttttcttt attcagtttt aggaagttta ttagcaaatt
     8641 atatctacga aaaatcttca aaataaataa ctttttcggt tctactttgt acactttatc
     8701 tacctggtag acctaattgt tattgcttta caggtctaaa attcgtcgtt ataatctagt
     8761 taaatcccgt aagggcgcac ttatacgtcg gataaatcga catgtgcaaa aacgtaatcg
     8821 accttcgtcg atttttttat ttcaaaatta aagaaacggc ttgagatatt ccgagcagtt
     8881 ataaaaatta atttgcgttg aatattgcta ctacattctt gaaaaagtga tgtagattta
     8941 tagcccataa cttttttgaa ttacatcgac gatacggcgt cgaaagaaaa ataaatagac
     9001 cgtatgaaag aaaaagctac ctcactttat ctggggtagc ttttattttt tatgccctta
     9061 ttttgaattt taagagggca ttttaaagat ttaggggtga ttcatatagt tttatatcta
     9121 aaacttatat atgcttttaa aacgcaaata tgagccaaat aaatatatgc tattttcaaa
     9181 aacaaaaatt tgagcaaaaa caataactat ttttagacac tgcccattta catgcaaatt
     9241 aaaaattagc ataaaattct ttaaaacttc cccacgtttt tctaaattcc tgtagggcgt
     9301 ttttatcaaa aatcaagaca agaagaaact cgttcacact cgtttcaaaa aaacaagaaa
     9361 tctaatctat acacctaccg ttttccaacc ccctcaaagc atacaaatta aaaacaaaat
     9421 tattcataat cttactttct tttaacaaaa acataaaaac cataaataga caaattaact
     9481 atcaaaggca ataaacagta taaaaaaacc acgaataaat cttcatcaac atctaaaata
     9541 aaaaagaata aaacaattaa caaaatacta attaacaaac ctaaaagtgg caaacttttt
     9601 atatattttt ctaaattttc attcataaaa tagactcctt tcaaaaatat gtttataaat
     9661 atattaaaat aaatattgat tttataaagc gcttacttat acaattaaaa tatcttatta
     9721 tcttggaggt atcaaatgaa aattataaaa ttattttcaa taatttcctt gttcctactg
     9781 gtatttagcg cgaatttttc taatgcttac gcaaatgaag aacaaaaaag ttctttacta
     9841 gaaaatcaaa aagaaaaatt gaaaagcaaa aacatcgaag tatcacaaat gaccaagaaa
     9901 gaaataagtt ttctagaaca attgcaaaat aaaaatgaag atatgcaaaa attcgaaaag
     9961 caaatgaaaa aagaaggatt taaaaaaata aagctagaag acaaaaattc taaattaaaa
    10021 tatctagcta cagaagcaaa tgataaaaat gcatatggct acatagctaa taacgcttat
    10081 aaaaatgaaa aaacaaaaga aattgtagtt tctgaaacaa tatatgataa ctacaacaaa
    10141 aaaatcacaa aattcattgc cgaaaaaaga agtatgactg aaaacaaaga gggaaaaata
    10201 cttattaaca aaaattacgt tgaatataag gctgaaaaag ttgataaaaa cagaccggta
    10261 acatatgcag gattcaaatg gaacggcaaa gcattcgctt gtagtatggc tggactttat
    10321 gcatgtgctc actactgtgg agtatgggca atcgtaaatc caatagccgg cggtgtatgt
    10381 gaagcagttt gcggaactgc atttgcagct ggatgctctg taggttgatt ttattccaat
    10441 tgttttattg acgttgagcc tcggaaccct taacaatccc aaaacttgtc gaatggtcgg
    10501 cttaatagct cacgctatgc cgacattcgt ctgcaagttt agttaagggt tcttctcaac
    10561 atcaataaat tttttcggca taaatacgat gttccttatt tatcctcata aaaaacaata
    10621 gctgaaatat ataaccaatt actgtgtgtt tgaatttcta atacatgaat atcattcttt
    10681 aaaaacctat taacttgttt atctaattga cgatgagtaa aagtagcgaa agttttaatc
    10741 ttcatgatat ctctcctttt ataaaaatag caatccttat tacaatatta aacactttta
    10801 ttttggattt taagaggcat tttgaagact tagaggcaaa tcatataggt tttgtactta
    10861 aaaatctata cagtctctta aaacgcaaat atgagccaaa taaatatatg ctaatttcac
    10921 aaacaaaaat ttcagcaaaa tcagtgacta attttttaga tactgcccat ttacatgcaa
    10981 atttaaaaat tagcataaaa tactttaaaa cttccccatg tttttctaaa ttcctgcagg
    11041 gcttttctta tcaaaaactt agaaatttta atttaactct aaaatctccg taagagcata
    11101 agggaaattt gctatcattc aaaaatgata gatctggcaa ggccagacat tccaagggtt
    11161 ttaagttatt ttaaagtgat atcatacaaa gctgtagtgt cacacttttg tgacactaca
    11221 tacagcattt gtcacaaaaa taagacagca cttttttaca gaaaaacaat aaaaaaagac
    11281 atcagttatc actaatgtct taaataataa ttttgctttt cgaaattcca aattgcggta
    11341 attgaatgtg ctttttttct tcgtctgtcg ttttatcttc atcacttgta ttaatcaaat
    11401 taccatcttc ggcatcgtct aagtttaaaa ttttatgctt ttgtttaagc aatccaccat
    11461 aacttaacat acgttttcga tacaaacctt tttctaaatc acttacaatt tcttgatttt
    11521 tttcgtcgtc atcagttaaa aagtcagatg atttaacgga atattttgat gtctcttcga
    11581 gtgttataaa atcatttgaa ttattgatta aagatattat attgtttcat gtttttacgg
    11641 cgcatataaa gggcatgtgt ttcttcaagt tcagttagaa ttgtttgcat taaataagct
    11701 tgcattgact tgattgtttc cttttggcct ttaactcccc tatcatgtat agctttttta
    11761 agtcttttta aagaagttaa tatttcatcc tcaatgtcct ctaacatgta atgcatatct
    11821 tcatgccttg aattaaatga tttttttgct ttaagaataa cagcctttat gatttctaaa
    11881 tctctataat catattttaa taaataatta gttagttgtt ctgggaattc ttgcaatagc
    11941 atttctttat catcaaaatt atgtgtaaat tgatttgaat gatttgaatg attactaatt
    12001 tcatttttgt actttatatc attcaagtca ttcatatcat tattctcagt atcattaaag
    12061 tcaatgtcat taaagtcagt atcattagtg ttcgattttc gaacttctgg acgttcgatt
    12121 ttcgaacttc tggacgttcg attttcgaac ttctggacgt tcgattttcg aacttccttc
    12181 tcgccgtaag gttcaacatc gttttcttct ttttctattt tgtaaatatc acgttctgtg
    12241 acaataggtt tcattaaata tagaatattt ggtttattta aaccacgtcg tttttgaatg
    12301 agtagaccat cgttttctaa cccttttttt atttttgtta acttttcttt tttacaattt
    12361 aagatttcca ttagcttttc atttgaataa acaaagtaaa tgttaccatc ttcatcaacc
    12421 cagctatttt taatagataa atttaagcga tcacgtaaga tagcataggc tattttcata
    12481 tcattagtta gatttttata attttcactg gtaaagaata ctttagggat ttgataaaat
    12541 ctttctttgt aattttcttg cactgtaaaa tattgatttt gcatgacaaa aaacctcttt
    12601 ccatatttta gtttggtaag aggtaataaa acgtttttat ttagctttac atttatttaa
    12661 tgatttaggt gcttgcacta agatattatt cattatataa tacaagtatc gaaaattata
    12721 taaataaaca gtttctaaac gttttattac caaaccccga ctattaccag tagttggggt
    12781 ttttgttata ttaagtttat tatagcactt taaataaact tataaaaggg aaaatatagc
    12841 tttatcaagg gtttataaac ccttgataaa tatcataaac ctttcataaa cccttgataa
    12901 aaaggttgat ggatgtttat ttttatttat taatcacttt attattaagc attataagct
    12961 tataatattt tatcaagggt ttataaacat tttataaagg gttgatgata tgaaaagtgt
    13021 gaaaaagttg agtgaagaat taggggttag taagcaaacg atttttaata atattaagcg
    13081 attgaatata gagacaataa aacaggaaaa cacttcgttt attaaagaag ataccgatat
    13141 agaaaaaatc attcaaagag taaatgaaaa taaaaagaaa tatggttttg aaagtactac
    13201 tgaagacaaa caaaaaaaag ggtcggataa tattaatttt gaatcgaaaa gcgatgctca
    13261 aatagtagaa atccttaaaa atcagattga tactttaaat aatcaaattg aaaaacaaga
    13321 aagtcgtcat gaaacaacaa ttgaatttta cagaaaagag ttgcaagaaa gatcaaaatt
    13381 gcttgaaaat cagcaagttc tggcattaga gagtaataaa aagattcaaa aattagaaaa
    13441 tcaattagaa aaagaaaaac accttaatta tccctttgat acatctacta acactagaca
    13501 aaacgttgat gcacaagaaa aaacatatac tactaatcct gtaaatatta acagagatca
    13561 ggaagaaact aaagaaacag agattcaata taaggatata tctggaagtc aaagcgacga
    13621 aagtacacaa ggtgaagaag caaaaaaaga agacgttcca gctaatctaa atgacaatga
    13681 tagtgatata gaagaaaaga gtgaagaaac ggaacctaaa aaggggtttt ggagtcgttt
    13741 gtttggtagc taaaataatt gattaggcac taaaagttat aaaattttag tgtcttttat
    13801 atagtgagaa cttctggtaa tcatcgatta tgtaaactaa agtatttcaa ataattaact
    13861 gtattaatac tctcatcatg attgcgttat aaacgttcgt tattgagaca aaatttaata
    13921 gtactactac acaaaaagag agtgcgaatt cgcactctct ttttgtgata aatgaatggt
    13981 taagaaaata attcatccac agttgtatca aacaatcttg ccaacttaaa tgctaatgtc
    14041 aatgtaggat catatttatt gttttctatt gcattaatag tttgcctaga cacttttaat
    14101 tctttactta aagtttcttg agaaattcct ttttgttttc tatattctct aattctattt
    14161 ttcattaatt taacctttag atgtatattt tttatttgta gtatagtaat taaacagcat
    14221 aagaaatagt gctacaatta taaagaaaat ttctggatat tgagaaaacc aagatccttc
    14281 aatacttggt gacaaacctg taaatttaag cactagtgta aataacatac aatatacaat
    14341 ccaaataaaa cttttagcca gtatttcaat aaacatttta cgttgtcttt catcgtatcg
    14401 atcttgttgt ccttttttat ctaatgtttc tgaaggtaaa tatttgttag ttatagccgt
    14461 ccataaacaa gaaaaaatta aaataataat aaaaggaatt aaattcatta taacacctca
    14521 taaatgtaaa aagtatttga catttatgag tgtaaatatt tttgatacaa atgtcaaata
    14581 ctttttacat tataatatat tttaaaacac cttattagta aaacaattac tatatagaaa
    14641 gctgcgaact cgtactcttt tttcttgtat aattattaaa atctcaattc aataatatgt
    14701 tatggaattt gagacactgt gagagacgct catttgcatt gacaatcaat ttatcaagat
    14761 aatcccttat catgtttaat tctataaact gtttgtcttg tgatattaac ttctttcgca
    14821 atcttactaa tcgctttgcc ttcttctaac atttccacca cgcgatgata aataacacgt
    14881 ttctgtgggt ctttagcatt tggtgaataa agtaaagggc gccctttata tacgcctttt
    14941 tccttcgcaa cttgaatccc ttgcgcttgt cgacgtttac tttcatttct ctcttgttct
    15001 gaaaccattg ctaatatttg aatgattaaa tctttcataa acttatccag taatggattg
    15061 ccaattactt cattcatcat aggtaagctg gtaatcatta actgaacgtc tttttctttt
    15121 aaataattca cagtttcaat aatttcatca tagttacgtc ctaaacgatc aatagattcc
    15181 acaacgaatc gatctcccat tctgacaaaa ttgagtgctt cttgaaatac aggtctattt
    15241 tccactgact ttcccgactg tttctctgta aagattttct ccacaccaaa cgtctttaaa
    15301 ttatccaatt gtctttctaa attttgatct atcgatgata ctctcgcata acctataatc
    15361 attcatcttc actccataat aaatatacac cctaatcata cgttttaatt gttataattt
    15421 caacaattct ttttgtatgg ttagggtgta ctcaaagtgt acatatctat aattaaaact
    15481 tactaaaaat aaatatagat tttttctttg taacagaacc gttcatttga aggtcatctc
    15541 tatcataaat atttaaatta actaaaaatt ttaatcatct gtttaatact tatgaaatgt
    15601 atttacggta ggtattaaaa ttgatgaaat ttatataagt gctttcaatg aaaatgaccg
    15661 tttatttatc ctcctgattt aaggagttag aaatttcgaa aacgattctt tccatttgtt
    15721 gcatttgttc catgtccaga tatttaatac attatagaaa accaaagacc atcactaatt
    15781 aaacgcagtt ttaaaatttc atcaggattg ccttcgtctt taaaatgggc ctcttttaat
    15841 ataagttcta ctctttcata cctaactctt tcaatgccat cgtttcatac gccgattctc
    15901 ctgattccat agcaaactca gcgataaaca ttgggaatac tgcattgagt tctattatat
    15961 gctctttcat tcgtggccat atttcacctc cagctttacc gtatgctgtt ataagttgct
    16021 ctaatccttc gtcgtcgaat acacgatggt gtcctataaa gtccattgat gggtcggagt
    16081 gggttgcttc agtccagtct atgagacctg tgacgtttgc ttggttatct accattatat
    16141 gtcctggatg taagtcccca tgtatcatgg tcgcatgtcg aggccatagt tcgtcgtttt
    16201 ctaaccattg tttccatctg ttccataatt catctgatac gccataagtt tctttaactt
    16261 tattcattct tctttgaaag tcatttttta tttcttgtat ggttttgata tttatatgct
    16321 gaacgttaat gttttcttct ggtatgttgt gtaaatctac gagtgtttca gctaatgtgt
    16381 taataaagtt ttctggtaat ggtttgtgtt caatttccca tacataattt tgtatttctg
    16441 gatctattgt ggctgcgggt ttacctgtaa gttttgggta agcaataagg tcttttgcat
    16501 gtactttcca cttcggtatt tcaaatgaaa cattcttctg taagaagtct accgtttgtt
    16561 tttcgggttt tgttcgttta taaacgtcag gtcttcttgg tagtctcaaa acccattcta
    16621 ctccatgttt atcttttcca aatgcaactt gaaaatcaag tcccgattca ttcaatgaga
    16681 ttgtttgagg ttggatgtgt aattgatatt tttctgcaca tttaataatt tcattatgtc
    16741 gagtcatttt attggatcct tatttctatt atatttttct tgtgaacgta ttttttatat
    16801 tgatataaat gaacctaata ttcaatataa attagtgtat agtttttaag ttatatcatg
    16861 aagagattgt cctgttaatt cccatttttt atctgataca tgtttaacaa accttgtatc
    16921 atgtgatgta aacaaaatga ttccaggata tttattcata aacatttcta atgcttctaa
    16981 tgttttaata tctaaaaaat tagttggttc atccaaaatt aacatattcg ctttcgttga
    17041 aaataatact gctaacgata atttcgttct ttccccacca ctcaaaacat tacaagaacg
    17101 atcaagtgct tcatttaaac ccaagttatt taaaatagct ctactgaatg attctgatga
    17161 atccgtttca tccattaaat attgcaataa tgaaacgtca cgcatgtctt cataagcaag
    17221 ttgacgatag tatgccattt gcactttagg agaacaatca attccctcta tttggtggta
    17281 aatagcttca agtaaagttg tcttacctac accatttgca cctacgagcg ctatattttt
    17341 gccatatggt atttggaatc gtacttgtgt tagcagtttt tgacttcctt taaccaatgt
    17401 tagattttgt gcaatgattg gataattatt atggatatca taaattttat tttgtgggaa
    17461 attgaattca tgataacttt gtggtttttc aacttcttcc aaatgttcca ttcttttttc
    17521 aatatgctta gcttgttttt gagcagcctt ctcaaccgtg cctttttgtt ttgatgcact
    17581 taaacgatct ggtgctatac ttttgttttt ttgttttgat gatgcttgtg ccatttgttg
    17641 cgcttgattt cgtttagctt tactggcttg ggacaatctt tgtttttcac ttatatactg
    17701 ttcatattga cgttgttgtt ctaactgttc ttgttcatat tgattttgat actgtgtgta
    17761 attaccttta aacactctta ttgtgccatc ttcttgtata tcccaaattg tgtcagcaat
    17821 ttggtctata agtgctctat catgacttac tataattaaa gtaccatagt aatattttaa
    17881 aatatttttc agataatctt taccaatttt atccaagtga tttgtaggtt catctaaaag
    17941 taatatcgga ctataatttg atatgacatt agctaattta tattttgctt tttcaccacc
    18001 actcatactg tcggttgtat gcataggtat atggagttca ctcattaaac taccgtctaa
    18061 cgtgttaaaa tcattttcca catccatgtt aagctgttca aaatattgaa tttcgccaac
    18121 cgtttcaact gttccagatt ctggttttgt tttttgagca atcatattta gtaatgtggt
    18181 tttgccagta ccattacccc caataagtgc tattttttca aattggtaag cgtaaagatg
    18241 accgatgtta agtgatcgta aatctgtcaa tttatgattg atttggttaa atttaattgt
    18301 atattgttcc atatttatac actccttgta tatttaaagg acgtgtacgc aaaaaaatga
    18361 gacaggctac tcccgtctca ttcacattac actttcaaac acttaaaaat ttattatgat
    18421 gttttagtat ttgaaagcgt ttattattcc tatcgaagga taaaaaatga cataagtcat
    18481 cctatgagtg tgtattgcgg acaagccttt ttgaattaat ttagttttgt attcaaaaag
    18541 gtttatttga gtctcataga agctgtcatt ttaatcctcc ttcatcgata aaatagtaat
    18601 gttaatataa cattttgttg gtttttttac aatatatctc taagtagaaa gattatataa
    18661 caaaagtata aaaatacaat tttagattga ttatacatct ccttaattta gagtgtactc
    18721 ataaaata
//
LOCUS       DAKTIP010000031        18627 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAKTIP010000031 DAKTIP010000000
VERSION     DAKTIP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 18627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.031"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            185..2707
                     /gene="mprF"
                     /locus_tag="O0305_000855"
     CDS             185..2707
                     /gene="mprF"
                     /locus_tag="O0305_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCY0274009.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGEFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2910..3419)
                     /gene="msrA"
                     /locus_tag="O0305_000856"
     CDS             complement(2910..3419)
                     /gene="msrA"
                     /locus_tag="O0305_000856"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0274010.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3556..4539
                     /locus_tag="O0305_000857"
     CDS             3556..4539
                     /locus_tag="O0305_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0274011.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4627..4812)
                     /locus_tag="O0305_000858"
     CDS             complement(4627..4812)
                     /locus_tag="O0305_000858"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCY0274012.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4960..6222
                     /locus_tag="O0305_000859"
     CDS             4960..6222
                     /locus_tag="O0305_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCY0274013.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6359..7450)
                     /locus_tag="O0305_000860"
     CDS             complement(6359..7450)
                     /locus_tag="O0305_000860"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCY0274014.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7615..8646
                     /locus_tag="O0305_000861"
     CDS             7615..8646
                     /locus_tag="O0305_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0274015.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGN
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9138..10544
                     /locus_tag="O0305_000862"
     CDS             9138..10544
                     /locus_tag="O0305_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCY0274016.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITELMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            10541..11107
                     /locus_tag="O0305_000863"
     CDS             10541..11107
                     /locus_tag="O0305_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0274017.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            11113..12111
                     /gene="trpD"
                     /locus_tag="O0305_000864"
     CDS             11113..12111
                     /gene="trpD"
                     /locus_tag="O0305_000864"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCY0274018.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALEKYHQMRGE"
     gene            12113..12895
                     /gene="trpC"
                     /locus_tag="O0305_000865"
     CDS             12113..12895
                     /gene="trpC"
                     /locus_tag="O0305_000865"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCY0274019.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12895..13527
                     /locus_tag="O0305_000866"
     CDS             12895..13527
                     /locus_tag="O0305_000866"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCY0274020.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13520..14734
                     /gene="trpB"
                     /locus_tag="O0305_000867"
     CDS             13520..14734
                     /gene="trpB"
                     /locus_tag="O0305_000867"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCY0274021.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGDDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEDGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMVERGLAND"
     gene            14727..15455
                     /locus_tag="O0305_000868"
     CDS             14727..15455
                     /locus_tag="O0305_000868"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCY0274022.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            15777..17039
                     /gene="femA"
                     /locus_tag="O0305_000869"
     CDS             15777..17039
                     /gene="femA"
                     /locus_tag="O0305_000869"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCY0274023.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            17058..18317
                     /gene="femB"
                     /locus_tag="O0305_000870"
     CDS             17058..18317
                     /gene="femB"
                     /locus_tag="O0305_000870"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCY0274024.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         6596 a         2646 c         3389 g         5996 t
ORIGIN      
        1 tatgaatcag tatagataac catattgttc tgtttgagat agaaatattt tctatttact
       61 tcttaataca gatatcaata tgacaaaagt gttatataaa tcaaaggtaa atgatatgct
      121 atttttatgg tttatatgat atattttgat ttataacaga aataattaga attgatgtga
      181 aaaaatgaat caggaagtta aaaacaaaat attttcaatc ttaaaaatta cgtttgctac
      241 agctttattt atttttgtag caatcacatt gtatcgggag ttatctggta ttaactttaa
      301 agatacgttg gttgaattta gtaagattaa ccgtatgtcc ttagtgttac tatttattgg
      361 tggtggggca tcgcttgtta ttctatcaat gtatgatgtg attttatcta gagctttaaa
      421 aatggatata tccttaggca aagttttaag agtaagttat atcatcaatg cattgaatgc
      481 gattgtaggt ttcggtggct ttattggtgc aggcgttaga gcaatggttt ataaaaacta
      541 tacgcatgat aaaaagaaat tagttcactt tatatcctta atacttattt caatgttgac
      601 aggtttaagc ttattatcat tgctaattgt attccatgtt ttcgatgcat ctttaatctt
      661 agataagatt acatgggtaa gatgggtatt atatgtagtg tcatttttct taccattatt
      721 cattatttat tcaatggtta gaccacccga taaaaacaat cgttttgtag gattgtactg
      781 cactttagtg tcgtgtgttg aatggttagc agctgcagtt gtattatatt tctgtggtgt
      841 aattgttgac gctcatgtat cattcatgtc ctttattgca atatttatca ttgctgcatt
      901 atcaggttta gtcagcttta ttcctggtgg tttcggcgct ttcgatttag ttgtattact
      961 aggatttaaa actttaggtg tccctgagga aaaagtatta ttaatgctac ttctatatcg
     1021 ttttgcgtac tattttgtac cggtaattat tgcattaatt ttatcatcat ttgaatttgg
     1081 tacatcagct aagaagtaca ttgagggatc taaatacttt attcctgcta aagatgttac
     1141 gtcattttta atgtcttatc aaaaggatat tattgctaaa attccatcat tatcattagc
     1201 aattttagta ttctttacaa gtatgatctt ttttgtaaat aacttaacga ttgtttacga
     1261 tgctttatat gatggaaatc acttaacgta ttatattcta ttggcaattc atactagtgc
     1321 ttgtttatta cttttactga atgtagttgg tatttataag caaagtagac gtgccattat
     1381 ctttgctatg atttcaattt tattaatcac agtggcgaca ttcttcactt acgcttcata
     1441 tattttaata acatggttag ctattatttt tgttctgctt attgtagctt tccgtagagc
     1501 acgtaggttg aaacgcccag taagaatgag aaatatagtt gcaatgcttt tattcagttt
     1561 atttatttta tatgttaacc atatatttat tgctggaacg ttatatgcat tagatattta
     1621 tacgattgaa atgcatacat ctgtattgcg ctattacttc tggcttacga ttttaatcat
     1681 cgctatcatc ataggtatga ttgcatggtt gtttgattat caatttagca aagtacgtat
     1741 ttcttctaaa attgaagatt gcgaggagat tattaatcag tacggcggta attatttgag
     1801 tcacttgata tatagtggtg acaagcagtt tttcactaat gaaaataaaa cagcattttt
     1861 aatgtatcgt tataaagcaa gttcattagt ggttcttgga gatccgttag gtgatgaaaa
     1921 tgcctttgat gaattgttag aagcattcta taattacgct gagtatttag gctatgatgt
     1981 tatattctat caagttacag atcaacacat gcctttatat cataatttcg gtaaccaatt
     2041 tttcaaatta ggtgaagaag caattattga tttaacgcaa ttttcaactt caggtaaaaa
     2101 acgccgtgga tttagagcga ctttaaataa attcgatgaa cttaatattt cgttcgaaat
     2161 tattgaacca ccgttttcaa ctgaatttat aaatgaactt caacatgtaa gtgatttatg
     2221 gctagataat cgtcaggaaa tgcatttctc tgttggtgaa tttaatgaag aatacttatc
     2281 taaagcgcca attggtgtaa tgcgaaatga agaaaatgaa gtaattgcat tttgtagttt
     2341 aatgccaaca tactttaatg atgccatttc agtcgattta attagatggt tgccagagtt
     2401 agatttacca ttaatggatg gtctatactt gcatatgtta ctttggagta aagaacaagg
     2461 ttatacaaaa tttaatatgg gtatggcaac gttatcgaac gttggtcaat tgcattattc
     2521 atatttaaga gaacgacttg caggccgtgt ctttgaacat ttcaacggtc tatatcgttt
     2581 ccaaggatta cgtcgttata aatctaaata taatccgaat tgggaaccac gctttttagt
     2641 ttatcgtaaa gataattcgc tttgggaatc actttctaaa gtaatgcgtg taatacgtca
     2701 caaataatta aaatccaagt gctaagaggt atacagttat gcctgatgcg cttggatttt
     2761 ttggtttcta taaagaatga tttataaaaa acggtacaga taagaatatt taagtttcct
     2821 atctgtaccg tattttaata ttgaaatgag ctaaagttaa gtcgcctaac ttttattatc
     2881 agtccaatca gtttcatcaa gtataaatgt tattgcttat ttttgtattc ttggcgtatt
     2941 ttttgttctt ctgcatagcg ctctggattt ttcttataaa aatcttggtg atagtcttcg
     3001 gctttgtaaa attgtgacgc tggtaatatt tttgttgcaa ttgccttatc agcattaatc
     3061 gtatttttaa gctgctcgat ataagtctca gcgagttctt tttgatgatc attagtgtag
     3121 aaaatagctg tttgatattg aggaccacgg tcttgatatt gaccacctgt atctaatggg
     3181 tcaatgactg agaaaaatat ttctaataac ttattgtatg agaataatgc aacatcatat
     3241 tgaatttcaa cagtttctaa atgaccactc gtacctgatt ttacttgttc gtaagtagga
     3301 ttttcaatat gtccgcccat atatccagaa gttacttttt ctatgccgtc aaaggtgtca
     3361 aatggtttcg tcatacacca aaagcaacct ccggcaaaat aagctgtatt aatattcatt
     3421 tttgacatcc tttcattaga ccttagtacg atttattaag aaatcacttg ctttttgaat
     3481 tgtttttata taacgttaat atgtgattat tatagtacac aaaaatctat ttttgaagat
     3541 ataaggtagg taaatatgga taaagaaact aatgacaacg aatatagacg tcaaagtgaa
     3601 catcgcactt cggcgcctaa gcgaaaaaag aagaagaaaa ttaggaaatt acctatcatt
     3661 cttctgattg ttgtaatttt acttatcgca ttagttgtat atattgtaca tagttacaat
     3721 agcggtgtag aatatgccaa gaaacatgcg aaagatgtta aagtacatca atttaatgga
     3781 ccagtaaaaa atgatggtaa aatttctatt cttgtactcg gtgcagataa agcacaaggt
     3841 ggacaatcaa gaacagattc tatcatggtt gttcaatatg actttatcaa taaaaagatg
     3901 aaaatgatgt ctgtcatgcg tgatatttat gcagatattc caggatatgg aaaacacaaa
     3961 attaattcag catacgcttt aggtggtcca gagctactta gaaaaacact tgataaaaat
     4021 ttaggaatta atcctgaata ttatgcagta gttgatttta ctggatttga gaaaatgatt
     4081 gatgaattaa tgcctgaagg tgtaccaatt aatgtcgaaa aagatatgtc gaaaaatatt
     4141 ggtgtatctt tgaaaaaggg taaccatagg ttgaatggta aagaattact tggttatgca
     4201 agattccgtc acgaccctga aggtgacttc ggacgcgtgc gacgtcagca acaagtgatg
     4261 caaacattga aaaaagaaat ggttaatttt agaacagttg ttaaattacc aaaagttgca
     4321 ggtattttaa gaggctatgt gaatacaaac attcctgatt cagggatttt ccaaacaggt
     4381 ttgagttttg gtatccgagg tgaaaaagat gttaagtcat tgactgtgcc aatcaagaac
     4441 tcatacgaag atgtcaatac aaatactgat ggtagtgcat tacagattaa taaaaacaca
     4501 aataaacaag ctattaaaga ctttttagat gaagattaaa aataaacaag gcgatttcta
     4561 tcatactcag atagaagtcg ccttgttcgt tttcaaagaa ttaattggca tgcatcttat
     4621 gaagaattat tgatctgact ttcttacgcc agccacacca taatggtttg gtttcatttc
     4681 ttctataaca acgtgaattg cttgtctatt tgcccccgtt gttttttcta cggcgtcagt
     4741 tacttcgcta actaaatttt ttaattgttc atccgaacga ccttctaata attttacatt
     4801 gacgattggc atcatccaaa cctcctaatc attattaatt tgagtatatc atgtaatact
     4861 taaaaattca tttgcaaaat aacagaacat ttgttctaaa atagttgaaa tagaacacgt
     4921 gttcgtatat aataagattc aagtaaggga ggaataacag tgtataatta tcatttatta
     4981 gaagataggg atgttctatg tattgaccaa aaaagttttt ttgcgagtgt ttcttgtatt
     5041 gaaaaggggc tagatccatt agaaacaaag ctagctgttg ttgcagatac taagcgtcag
     5101 ggttctgtaa tattggctgc gacacctaaa ttaaaagaat taggcatcaa gacagggtcg
     5161 cgattgtttg aaataccaca tagaaatgat atttacatta tcaatccaag tatgcgtaaa
     5221 tatcttaatg tttcagttgc tatttctaag attgcattgc gttatattcc acctgaagat
     5281 ttacaccaat atagtattga cgaatttttt atggatgtta ctgatagcta tcatagattt
     5341 agttctacag tacatgcatt ttgcgaaaga cttaaacgtg aaatttatga agaaacaggc
     5401 atttattgta ctgtgggcat tggttctaat atgttattaa gtaaaattgc tatggatgtt
     5461 gaagcgaagc atagtcaaaa tggtatagct gaatggcgat atcaagatgt accaacgaaa
     5521 ttatggccaa ttcagccctt gcgagatttt tggggtatta atcgtcgaac agaagccaaa
     5581 ttgaataaaa gaggaatttt tactatagga gatttagcga aatatccata taaattttta
     5641 aaaaaagagt tcggtatttt aggtgttgat atgcatctac atgcgaatgg gatagatcag
     5701 agtaaagtac gtgaaaagca caagatcagc aatccatcga tatgcaaaag tcaaatatta
     5761 atgagagatt atcattttga tgaagcaaaa gtagtaatgc aagagttaat tgaagatgtt
     5821 gctagcagag ttcgagcaag aaaaaaagtg gcaagaacga tacattttgc ctttggctat
     5881 agtgatgaag gcggtgtaca taagcaatat actttgaaag atccaacaaa cttagaaaaa
     5941 gatatttata aagtagtaat gcatttcgca gataaattat gtaataaaca agcactatat
     6001 cgtacgctaa gtatatcttt gagtcaattt attaatgagg atgagcgaca gttaagtctg
     6061 tttgaagatg aataccaacg caaacgtgac gaatgtctag ctaaaacgat agaccaatta
     6121 catttgaaat acggcaaagg tattgtgtcc aaagcagtat cgtttacaga agcaggtaca
     6181 aaacacggca gattaggttt aatggctgga cataaaatgt aatgactata cggtttaagt
     6241 aatatataac tgtgattcgt ataaaataag tctctaaaga taaatatttc atatatcaca
     6301 atagattttc acaataatat ctaagaatac atggaattta tcaaaagaga cttaataatt
     6361 attggatata acaatcaaaa tcactcaatg cttgcatacc gcgttctcgg tcagtagggt
     6421 ttttgaaact aatttttaaa gcaccgtata tatcttcgcg tacttctaag attcttaagt
     6481 tgcttataga tatgttatgt aaactcagga tataagtcac tttacttatc atacctgatt
     6541 catccggaat gtctacatat agatcatacg cagtatttag tccacctagt tgtttagcgg
     6601 gtagtgcgtc gcgatacgat ttagcttggg caaaaaatga taacaatttt tcagaatcat
     6661 tgctttcaat tagtctttct aaatcttgaa actgactttt tagctgtcga atcatttcta
     6721 aaatatacgt tttattactc aaggtgatat ctttccacat ttgtgcatta ctactagcta
     6781 tacgagtgat atcacgaaaa ccaccagctg caagtttatt aactaaatga tgttcttgac
     6841 cgttcttttg actaacatga actaaactag atgcaacgat atgaggtaaa tgacttacga
     6901 cgcttgttac gtagtcgtgt tcttcagcag tagttacaat aaatttagca agagtaggtg
     6961 ataacagttc ttttaacgtg tttgctgctt gctcatttct tggctcattg tagactaaaa
     7021 tataataagc gttttcaaat aagtgctttt tagcatttag tacaccagat ttatgactac
     7081 cagccattgg atgaccactg actaaatgaa tattatgctt taataaattg cattcgtgtt
     7141 gctgtatcat tgctttagta ctaccagtat cagaaacaat aacaccaggt ttagttggca
     7201 tatctataag ctcgctaaga tatttatttg tgatagcaac aggtgttgca taaataatta
     7261 catcggcttt tttaatagct tcactataat ttaaacattt ttcattaata atgccgattg
     7321 atttagcttt atctaactga gaagtatctg catcgtatgc aataatatta gtattagggt
     7381 tatggtattt tatattgcta gcaagacttc caccaattaa tccaagccca acaaataaaa
     7441 ctgttgtcat ataaatcacc ttatttcgaa attttcagaa taataacatt gtaaatgagc
     7501 tgttgacaca gtgcaatagt aaataaaaat cgataatagc attaatagat aaacggagat
     7561 aaatcatcta caataaagag tatagtaaca caatggcaac ggaggggtaa atcaatggaa
     7621 ccaatattag aaatgattaa aacattaaca ggtattaata gtccttcagg agacacagaa
     7681 gaagcaattc aatttgtcga aaaatatgca aaagacttgg gttatcaaac aacactaaca
     7741 aataaaggtg cgttattaat aacagtgcca ggcaaaaatg atgaagtaca acgctgtatt
     7801 actgctcatg ttgatacttt aggtgcaatg gttaaagaaa ttaaagaaga tggtcgctta
     7861 gcaatagaat taattggagg attcacgtat aacgcgattg agggtgaata ttgccaaatt
     7921 aaaactgatg ctggtcaaat atatacagga acaatttgtc tgcatgaaac aagtgttcat
     7981 gtatatagaa ataatcatga aatacctaga gatcaaaagc atatggaaat aagaattgat
     8041 gaagtaacta catcagaaga agatacaaag agtttaggta tttcagtagg taattttgtt
     8101 agctttgatc cacgtacagt tatcacgtca tcaggtttta ttaaatctcg tcatttagat
     8161 gataaagcta gcgtagcgat gatactacaa ttactaaaga aattaaaaga agagcaaata
     8221 atattaccac atacaacgca attttatatt tctaataacg aagaaatagg ttacggtgca
     8281 aatgcatcaa ttgattcgaa aatcaaagaa tatattgcat tagatatggg cgcgttggga
     8341 gacggtcaag catcggatga atatacagtt tctatttgtg ccaaagatgc ttcaggtcca
     8401 tatcataagc aattgaaatc gcacctagtt aatctttgca aaataaataa cattccatat
     8461 aaagtagaca tatatccata ttatggttca gatgcttcag cagctttaca tgctggtgcg
     8521 gatatcagac atggtttatt tggcgctggc attgaatcat ctcatgcaat ggaacgaaca
     8581 catattgatt ctattaaagc gacagagaaa ttactatatg catattgctt atcaccaatt
     8641 gagtaaacaa ttagtgttga caaatgtgaa cgacctatgt aatataatga actataaaaa
     8701 taattagaat tttctaaaga aatagtagca gatatgaaac gtagcaaata gaaagctaat
     8761 gggtgatggg aattagcacg ccatatcttg tgaattggac tttggaaaac aattgaatga
     8821 gttttgaaag tgaacatgaa ttatgttaac taaggtggca ccacggtaac gcgtccttac
     8881 aggtatatgc gttatgtggt gtctttttat ttagacaaaa tgtagtagtt aattaaaggt
     8941 agcaacagaa agttagtgga tgatgtgaac taacaccgag attaatgaaa ttgggttttg
     9001 tctgcaacag aaaaattata tatagtaaag agtgaactat gaatatttcg aatattcggt
     9061 taatttaggt ggtaccacgc gtcagcgtcc tttatattga taaggatgct ggcgcttttt
     9121 tgaaaggagc gtatagaatg gatatatttt ataaaaaaat aaaagcaaat gtaacgcccg
     9181 aagttttagc acaacttcat tccaagaaga tcattttgga aagtacaaat caacaacaaa
     9241 ctaaaggtcg ctattcagtt gttatttttg atatttatgg cactttaact ttagataatg
     9301 atgtattatc agtaagtact ttaaaagaat cgtatcaaat cactgaaaga ccgtaccatt
     9361 atttaacgac taaaataaat gaagactacc ataatattca agatgagcaa cttaagtcat
     9421 taccatttat atctggatat gttgggacgt gtagctttga tttagtaaga catgaatttc
     9481 ctaaattgca atcaatacaa ttagaagatc acaagcagca cgatgtaagg ttatatatgg
     9541 ttgaacaagt ttatgtattt gaccattaca aagatgagtt atatatcatc gcgacgaatc
     9601 aattttcaaa ttcaacaaaa tcagatcttg agaatcgagt tattaagtct atcgaagact
     9661 taactaaaat ccaaccattc atgcctacac aagattttga ttttaaaact aaagaaattc
     9721 aatcaaacat ttctgaagaa agatttatcg aaatgattca gtatttcaaa gagaaaataa
     9781 cagaagggga tatgttccaa gttgtgccat caagaattta caaatatgcg catcatgcta
     9841 gtcagcattt aaatcaactt tcgtttcaac tgtatcaaaa tttaaaacga caaaacccaa
     9901 gtccatatat gtattatctt aatatcgatc aaccatatat tgtcggtagt tctcccgaaa
     9961 gtttcgtaag tgtcaaagat caaattgtaa caactaatcc tattgcaggt acgattcaac
    10021 gtggtgagac gacacaaata gataatgaga atatgaaaca actacttaat gatccaaaag
    10081 aatgcagcga acatcgtatg ctagttgatt taggacgtaa tgatattcat agagtaagta
    10141 aaatcggtac ctcaaaaatt actgaattaa tggttattga aaaatatgaa catgttatgc
    10201 atatcgtaag tgaagtcaca ggtaaaataa atcaaaattt atcgccaatg acagttattg
    10261 cgaatttatt accaacaggt accgtttcag gtgcaccaaa attacgtgca attgaaagaa
    10321 tatatgaaca atatccacat aaacggggcg tttatagtgg tggtgttgga tacataaatt
    10381 gtaatcataa cttagatttt gcattagcaa ttcgaacgat gatgatagat gagcagtata
    10441 tcaacgtaga agctggttgt ggcgttgtat atgattctat tcctgaaaaa gaactgaatg
    10501 aaacgaaatt gaaagctaaa agcttattgg aggtgagccc atgatcttag ttgtagataa
    10561 ttatgattcc tttacatata acctagtgga tattgttgct caacatactg acgtcattgt
    10621 tcaataccct gatgatgata atgtgctgaa tcaatcggtg gacgctgtta ttatatctcc
    10681 tggtccaggg catccattag acgatcaaca gttaatgaaa atcatatcaa cctatcaaca
    10741 caaacccatt ttaggtattt gtttaggggc tcaggcactg acttgttact acggtggaga
    10801 agtcattaaa ggcgacaagg ttatgcacgg caaagttgat acactaaagg ttatatcgca
    10861 tcatcaacat ctgttatatc aagatatacc agaacagttt tcaattatga gatatcattc
    10921 attaataagt aaccctgaca attttccaga agaattgaaa attactggac gtaccaaaga
    10981 ttgtatacag tcattcgagc ataaagaaag accgcattat ggtattcagt accatcctga
    11041 atcatttgct acagactatg gtgtcaaaat aattacaaat ttcattaatc tagtgaagga
    11101 aggatgaaaa ccatgacatt actaacaaga ataaaaactg aaactatatt acttgaaagc
    11161 gacattaaag agctaatcga tatacttatt tctcctagta ttggaactga tattaaatat
    11221 gaattactta gttcctattc ggagcgagaa atccaacaac aagaattaac atatattgta
    11281 cgtagcttaa ttaatacaat gtatccacat caaccatgtt atgaaggggc tatgtgtgtg
    11341 tgcggcacag gtggtgacaa gtcaaatagt ttcaacattt caacgactgt tgcttttgtt
    11401 gtagcaagtg ctggcgtaaa agttataaaa catggtaata aaagtattac ctcaaattca
    11461 ggtagtacgg atttgttaaa tcaaatgaac atacaaacaa caactgttga tgatacacct
    11521 aaccaattaa atgaaaaaga ccttgtattc attggtgcaa ctgaatcata tccaatcatg
    11581 aagtatatgc aaccagttag aaaaatgatt ggaaagccta caatattaaa ccttgtgggt
    11641 ccattaatta atccatatca cttaacgtat caaatggtag gcgtctttga tcctacaaag
    11701 ttaaagttag ttgctaaaac gattaaagat ttaggtagaa aacgtgcaat cgttttacat
    11761 ggtgcaaatg gtatggatga agcaacacta tctggtgata atttgatata tgaattgact
    11821 gaagatggag aaatcaaaaa ttacacatta aatgcgactg attatggttt gaaacatgcg
    11881 ccgaatagtg attttaaagg cggttcacct gaagaaaatt tagcaatctc ccttaatatc
    11941 ttgaatggta aagatcagtc aagtcgacgt gatgttgtct tactaaatgc gggtttaagc
    12001 ctttatgttg cagagaaagt ggataccatc gcagaaggca tagaacttgc aactacattg
    12061 attgataatg gtgaagcatt ggaaaaatac catcaaatga gaggtgaata atatgacgat
    12121 tttatcagaa attgttaaat ataaacagtc acttttacaa aatggctatt atcaagacaa
    12181 acttaatacc ttgaaaagtg tgaagattca gaataaaaaa tcttttataa acgcaattga
    12241 gaaagaacca aagctagcaa ttattgcaga aattaaatcg aagagtccta cagttaatga
    12301 cttacctgaa cgagatttat cgcaacaaat ctcagattat gaccaatatg gtgcaaatgc
    12361 cgtgtccatt ttaactgatg aaaagtactt tggtggtagt tttgaaagat tacaagcatt
    12421 gacgacaaaa acaacattac ccgtattatg caaagacttt attatagacc cgcttcaaat
    12481 tgatgttgct aaacaagctg gtgcatctat gattttattg atcgttaaca tcttatctga
    12541 taaacaattg aaagatttat ataactacgc tatatcgcaa aatctagaag tgttagttga
    12601 agtacatgat cgccatgaat tagaacgtgc ctataaggtt aatgctaaat tgattggtgt
    12661 aaataacagg gacttaaaac gatttgttac aaatgtggaa catacaaata ctattttaga
    12721 aaataaaaaa acaaatcatt attatatttc tgaaagtggt attcacgatg catctgatgt
    12781 aagaaaaatc ttgcatagtg gtatcgatgg cttactaata ggtgaggcgc ttatgcgttg
    12841 tgacaatcta tctgaatttt taccacaact gaaaatgcaa aaggtgaagt catgatgaaa
    12901 ttgaaatttt gtggctttac atcaataaag gatgttacag cggccagtca attacctatt
    12961 gatgcgatag gtttcatcca ttatgaaaaa agtaaaaggc atcaaacaat tacccaaata
    13021 aaaaagttag cgtctgctgt tccaaatcat atcgataaag tatgtgtcat ggtaaatcct
    13081 gatttaacaa caattgaaca cgtattaagc aatacgtcaa ttaacacaat acagttacac
    13141 ggcacagaat ctattgattt tatacaggaa attaaaaaga aatattcaag cattaaaatc
    13201 actaaagctt tagctgcaga tgaaaacata atccaaaaca taaataaata taaagggttc
    13261 gtagatttat ttattatcga cacaccctca gtgtcgtatg gtggtaccgg tcaaacatat
    13321 gactggacta ttttgaagca cataaaagac ataccttatt tgatagcagg aggcattaac
    13381 tctgaaaata ttcaaacagt taatcaactt aaattatcac atcaaggtta tgatcttgca
    13441 tcaggtatag aagtaaatgg gcgaaaagat atagaaaaaa tgacagcaat tgtaaatatt
    13501 gtgaaaggag atagagacaa tgaataaaca aatacaaaca gaagcagatg aattaggttt
    13561 ctttggtgaa tatggagggc aatatgttcc agaaacatta atgccagcaa ttattgagtt
    13621 gaaaaaagct tataaagagg caaaagcaga cccagagttt caaagagaac tggaatacta
    13681 tttatcagag tatgtaggac gcgcgacacc acttacatat gctgcatcat atactgaaag
    13741 cctaggtggc gctaaaatat atttgaaacg agaggatcta aatcatacag gcgcccataa
    13801 aattaataat gcgttaggtc aagcgttgct tgctaaaaga atgggcaaga agaagcttgt
    13861 tgctgaaact ggtgcgggtc aacatggtgt agctagtgct acggttgctg cattatttga
    13921 tatggaactt gttgtcttta tgggaagtga agatattaaa agacaacaac ttaatgtatt
    13981 tagaatggaa ttacttggtg caaaggttgt ggcagttgaa gatggtcaag ggactttatc
    14041 ggatgcagtt aataaagcat tgcaatattg ggtaagtcat gtagatgata cacattattt
    14101 attaggttct gcattaggtc cagacccgtt cccaacgatt gttagagatt ttcagagtgt
    14161 gattggtaaa gaaataaaat cacagatatt gaagaaagaa ggtcgacttc cggatgcaat
    14221 tgtagcatgt atcggtggtg gctcaaatgc aatcggtaca ttttatccat ttattaaaga
    14281 tgatgttgca ttatacggtg ttgaagccgc aggtcaaggc gatgatactg ataaacatgc
    14341 acttgcaatt ggcaaaggat cacctggcgt attacatggt actaaaatgt atttaattca
    14401 agatgaagat gggcaagtgc aactagcaca ttctatttca gcaggacttg attatcctgg
    14461 tattggacca gaacattctt attaccacga cattggtaga gtaacttttg aaaatgctag
    14521 tgatacacaa gcaatgaatg ctttaatcaa ctttacaaaa catgaaggta ttatacctgc
    14581 aattgaaagt gcacatgcac tgagttatgt tgaaagacta gcgcctacga tgtcgaaaga
    14641 agatattatt gtagtaacta tttctggacg tggcgataaa gatatggaaa caattagaca
    14701 atatatggta gagcgaggtc ttgcaaatga ctaaattatt tataccttat attatgggca
    14761 ataaagattt gattgaaaat gcaacattgt tgagtgaaaa tggtgcagat ataattgaaa
    14821 ttggagtacc tttctctgat ccggttgctg atggtccagt tatcatggaa gcaggtcaac
    14881 aagcgattaa acaaggcatc acgatagatt atattttcaa tcaattagaa aaacatggtg
    14941 atcaaattaa gtgtaactat gtattaatga cgtattataa tattatttgt cattatggag
    15001 aacaagcgtt ttttgaaaaa tgtcgagata ctggtgtcta cggcttaatt attcctgatt
    15061 taccatatga attatcgcag cgtttaaaac aacaatttag tcactatggc gtcaaaatca
    15121 tatcgttagt tgcgatgact actgatgaca aacgtataaa agatatcgta tcccatgcgg
    15181 aaggctttat ttatactgtg acgatgaatg cgacaacagg gcaaaacggt gcgtttcatc
    15241 cagaattaaa acgaaaaatt gagtcaatta aagcgatagc caatgtgcca gttgtcgcag
    15301 gatttggtat aagaacacca caacatgttg cagatataaa agaggttgca gatggcattg
    15361 tcattggtag cgaaatcgtt aagcgattta aatctaacac gcgtgaggaa atcattaaat
    15421 atttacaatc tatccaacaa acattgaata attaagttta cttgatttaa aaaaattagg
    15481 cgaatactgt ttgaaaaagt gaaaaacggt gaattataaa attgaataga aatgatacaa
    15541 aagtaatatg agcaaaccca aacgttcata ttactttttt tgaaattgta ttcaaaaatc
    15601 taaatattac tataaaagta tacgcaatta aagcgtttat gttttagttt taacattaac
    15661 tattgtatac ttatttagat tagatttatt atttttgaca tttgcagagg ggaaatagaa
    15721 aaactgcaaa tacggaaatg aaattaatta acgagagaca aataggagta atgataatga
    15781 agtttacaaa tttaacagct aaagagtttg gtgcctttac agatagcatg ccatacagtc
    15841 atttcacgca aactgttggc cactatgagt taaagcttgc tgaaggttat gaaacacatt
    15901 tagtgggaat aaaaaacaat aataacgagg tcattgcagc ttgcttactt actgctgtac
    15961 ctgttatgaa agtgttcaag tatttttatt caaatcgcgg tccagtgatt gattatgaaa
    16021 atcaagaact cgtacacttt ttctttaatg aattatcaaa atatgttaaa aaacatcgtt
    16081 gtctatacct acatatcgat ccatatttac catatcaata cttgaatcat gatggcgaga
    16141 ttacaggtaa tgctggtaat gattggttct ttgataaaat gagtaactta ggatttgaac
    16201 atactggatt ccataaagga tttgatcctg tgctacaaat tcgttatcac tcagtgttag
    16261 atttaaaaga taaaacagca gatgacatca ttaaaaatat ggatggactt agaaaaagaa
    16321 acacgaaaaa agttaaaaag aatggtgtta aagtaagatt tttatctgaa gaagaactac
    16381 caatttttag atcatttatg gaagatacgt cagaatcaaa agcttttgct gatcgtgatg
    16441 acaaatttta ctacaatcgc ttaaaatatt acaaagaccg tgtgttagta cctttagcgt
    16501 atatcaactt tgatgaatat attaaagaac taaacgaaga gcgtgatatt ttaaataaag
    16561 atttaaataa agcgttaaag gatattgaaa aacgtcctga aaataaaaaa gcacataaca
    16621 agcgagataa cttacaacaa caacttgatg caaatgagca aaagattgaa gaaggtaaac
    16681 gtctacaaga agaacatggt aatgaattac ctatctctgc tggtttcttc tttatcaatc
    16741 catttgaagt tgtttattat gctggtggta catcaaatgc attccgtcat tttgccggaa
    16801 gttatgcagt gcaatgggaa atgattaatt atgcattaaa tcatggcatt gaccgttata
    16861 atttctatgg tgttagtggt aaatttacag aagatgctga agatgctggt gtagttaaat
    16921 tcaaaaaagg ttacaatgct gaaattattg aatatgttgg tgactttatt aaaccaatta
    16981 ataaacctgt ttacgcagca tataccgcac ttaaaaaagt taaagacaga attttttagg
    17041 aagggaatta tcaaaacatg aaatttacag agttaactgt taccgaattt gacaactttg
    17101 tacaaaatcc atcattggaa agtcattatt tccaagtaaa agaaaatata gttacccgtg
    17161 agaatgatgg ctttgaagta gttttattag gtattaaaga cgacaataac aaagtaattg
    17221 cagcaagcct tttctctaaa attcctacta tgggaagtta tgtttactat tcgaatcgtg
    17281 gtccagtaat ggatttttca gatttaggat tagttgatta ttatttaaaa gagttagata
    17341 aatatttaca gcaacatcaa tgtttatatg ttaaattaga tccgtattgg ttatatcatc
    17401 tatatgataa agatatcgtg ccatttgaag gtcgcgagaa aaatgatgcc ctagtaaact
    17461 tgtttaaatc acatggttac gagcatcatg gctttacaac tgagtatgat acatcgagcc
    17521 aagtacgatg gatgggcgta ttaaaccttg aaggtaaaac acccgaaaca ttgaaaaaga
    17581 catttgatag tcaacgtaaa cgtaatatta ataaagcgat aaactatggt gttaaagtca
    17641 gattccttga acgtgatgag ttcaatcttt tcttagattt atatcgtgaa actgaagagc
    17701 gtgctggatt tgtatcaaaa acagatgatt atttttataa ctttattgac acatatggag
    17761 ataaagtatt agtaccatta gcatatattg accttgatga atatgtgtta aagttgcaac
    17821 aggaattgaa tgacaaagaa aatcgtcgtg atcaaatgat ggcgaaagaa aacaaatcag
    17881 ataagcaaat gaagaaaatt gcagaattag ataagcaaat tgatcatgat cagcatgaat
    17941 tattgaatgc aagtgaattg agcaaaacgg acggcccaat tctaaacctt gcttctggcg
    18001 tttattttgc aaatgcatat gaagtgaatt atttctctgg tggttcatca gaaaaatata
    18061 atcaatttat gggaccatac atgatgcatt ggtttatgat taactattgc ttcgataatg
    18121 gctatgatcg ttataatttc tatggtttat caggtgattt tacggaaaac agtgaagatt
    18181 atggcgtata ccgctttaaa cgtggattta atgtacaaat cgaagaatta ataggggatt
    18241 tctataaacc aattcataaa gtgaaatatt ggttgttcac aacattggat aaattacgta
    18301 aaaaattaaa gaaatagatg taaatgatgt tagggcttat agttattgat actataggct
    18361 cttttttata tgttttttaa taaagcctcg aataatgata tattaaatga caagtttaac
    18421 aagtgattgc aataaatata aatatgaatt gcacaaccta tttgtaaatg attgagttga
    18481 gtatactttt taattcattc aatatgactc aaaacaagcc aatatgtatt atatttttgg
    18541 tgaataaacc ttagcataac aatttatgtc tgattatata tgtgtgtgtt tgcaagtgtg
    18601 tgatatcaaa tgtcatattt gtctaat
//
LOCUS       DAKTIP010000032        17963 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAKTIP010000032 DAKTIP010000000
VERSION     DAKTIP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.032"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            64..513
                     /locus_tag="O0305_000871"
     CDS             64..513
                     /locus_tag="O0305_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCY0274025.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(594..1181)
                     /locus_tag="O0305_000872"
     CDS             complement(594..1181)
                     /locus_tag="O0305_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0274026.1"
                     /translation="MTIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSFI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            complement(1159..1257)
                     /locus_tag="O0305_000873"
     CDS             complement(1159..1257)
                     /locus_tag="O0305_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274027.1"
                     /translation="MPIINVGDLWDNEHNVYNHWGGLDINDYRSTY"
     gene            1314..2996
                     /locus_tag="O0305_000874"
     CDS             1314..2996
                     /locus_tag="O0305_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCY0274028.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWT
                     QGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(3125..3508)
                     /locus_tag="O0305_000875"
     CDS             complement(3125..3508)
                     /locus_tag="O0305_000875"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCY0274029.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(3510..4361)
                     /locus_tag="O0305_000876"
     CDS             complement(3510..4361)
                     /locus_tag="O0305_000876"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCY0274030.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITIVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(4354..5172)
                     /gene="panB"
                     /locus_tag="O0305_000877"
     CDS             complement(4354..5172)
                     /gene="panB"
                     /locus_tag="O0305_000877"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCY0274031.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            5245..6105
                     /locus_tag="O0305_000878"
     CDS             5245..6105
                     /locus_tag="O0305_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0274032.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HISFKNVCQAVVYISGQKKGDVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIAHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(6191..6895)
                     /gene="budA"
                     /locus_tag="O0305_000879"
     CDS             complement(6191..6895)
                     /gene="budA"
                     /locus_tag="O0305_000879"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0274033.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(7173..8132)
                     /locus_tag="O0305_000880"
     CDS             complement(7173..8132)
                     /locus_tag="O0305_000880"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCY0274034.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            8819..10267
                     /locus_tag="O0305_000881"
     CDS             8819..10267
                     /locus_tag="O0305_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0274035.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAITFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(10348..11685)
                     /locus_tag="O0305_000882"
     CDS             complement(10348..11685)
                     /locus_tag="O0305_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCY0274036.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            11943..12359
                     /locus_tag="O0305_000883"
     CDS             11943..12359
                     /locus_tag="O0305_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274037.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLMALVIPRIK"
     gene            12482..13372
                     /locus_tag="O0305_000884"
     CDS             12482..13372
                     /locus_tag="O0305_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCY0274038.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVESKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDNLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(13565..15061)
                     /gene="mqo"
                     /locus_tag="O0305_000885"
     CDS             complement(13565..15061)
                     /gene="mqo"
                     /locus_tag="O0305_000885"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0274039.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            15357..15686
                     /locus_tag="O0305_000886"
     CDS             15357..15686
                     /locus_tag="O0305_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274040.1"
                     /translation="MAYSILNLYSILIQYKCKINHKKDIQYLHPRKTLQLPLILKIIL
                     NLALIGIVIIVLTHTEFQLNAGDSNVFLLNWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(15746..17344)
                     /locus_tag="O0305_000887"
     CDS             complement(15746..17344)
                     /locus_tag="O0305_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCY0274041.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(17538..>17963)
                     /locus_tag="O0305_000888"
     CDS             complement(17538..>17963)
                     /locus_tag="O0305_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCY0274042.1"
                     /translation="YRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSLCVLNHL
                     YINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQAFYHWQ
                     NSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
BASE COUNT         5594 a         3345 c         2866 g         6158 t
ORIGIN      
        1 cttttgccat tttttaatgt aacatggtgt tagtaataaa aataatacat tgaggtgttt
       61 tacatgacag cattattccc ttatatcgct tttgaaaatt caaaagaagc ccttgcatat
      121 tacgaagaag tatttggtgc aactgacgtt aaacgtttag aagttggcga agaacaagcg
      181 tcacattttg gtatgactaa ggaagaagcg caagaagcaa ctatgcatgc tgaatttgaa
      241 gtgcttggcg taaaagtgtt atgttctgat tcttttggtc gcgctgacaa aattaataat
      301 ggcatatcat tattaattga ttatgatgtt aacaataagg aagatgctga taaagttgaa
      361 gcattctatg agcaaattaa agatcattct tcaattgaaa tagaattacc gtttgctgac
      421 caattctggg gtggcaaaat gggcgtcttt accgataaat acggtgttcg ttggatgtta
      481 catggtcaag actatactgc aatccaacaa taatgttatt gataaagata tcatttaata
      541 agcttatgtt atttagcaag gcaagccact ctcgattcat ggcttgcctt attttattgt
      601 atatttttaa ttatcgttgc tggcccttga ttgacaatcg tattaaaatg ccttattaag
      661 tcaactttgt ctatacggtt tggatcttct acccaatgtc ttataaaaga caatcccgca
      721 cctgaaacat aactcatgaa ataagaaaat ggtataccat taatttgatc attttcattt
      781 ttataacgtt gtaaatgaca cataattaaa tcataaagtt tttcgtggat atttgaatct
      841 ttacccatac gaaacatcaa ttgataaaat gcgatgtctt tttctatcat ttctattaaa
      901 acggtcataa tttgatgtat gttatccgtg gataacttaa ctgctccatt taacttctca
      961 tcatgaatga agtctcttat ttcctccaac tgctggtcct ctaatttttc aagcaaatca
     1021 tacttatcat aataatgcgt ataaaatgta ctacggttaa catcagctaa atctgcaatt
     1081 tgttgcacag taatctcttc taattggtgt tgatgtaaaa gttcaataaa tgcatttctc
     1141 attgcaactt gtgattttct aatacgtcga tctatagtca tttatatcaa gtcctcccca
     1201 atgattataa acgttatgtt cattatccca caaatctcca acattgatga ttggcacaca
     1261 atgtttacct gtttaatata ggtgatacaa acaaacagaa aaaggtgata acaatgaacc
     1321 aacatttact aggaaatcca aaattaactg taactcatgt caatgaagtt aaagccggta
     1381 ttaaccacat cgttgtcgac agtgttcaat atggaaatca agaaatgatt atggaaaaag
     1441 atgtcactgt ggaaatgcgc gatggcgaaa aattatatat taatattttc agaccaaata
     1501 aagatggcaa attccctgta gttatgtctg cagatactta cggtaaagat aataagccta
     1561 aaatcacaaa tatgggtgcc ctttggccaa cattaggtac cattccgaca tctagtttta
     1621 cacctgaaga atcaccagac ccaggatttt gggtgccaaa tgattatgtt gtagttaaag
     1681 ttgcattacg cggtagtgac aaatccaaag gcgtcttatc tccatggtca aaaagagaag
     1741 cggaagatta ttacgaagtg attgaatggg cagcaaatca gtcatggagt aatggaaata
     1801 tcgggacaaa tggtgtttct tatcttgcgg tgactcaatg gtgggtcgca tcattaaatc
     1861 caccacattt aaaagcaatg attccttggg aaggcttaaa tgatatgtat agagaagtag
     1921 cctttcacgg aggtatacca gatactggct tttatcgttt ctggactcaa ggtatttttg
     1981 cgagatggac agataatcca aatatcgaag atttgattca agcacaacaa gaacatcctc
     2041 tgttcgatga tttttggaaa cagcgtcaag tgccattatc acaaattaaa acacctctac
     2101 taacatgtgc tagttggtct acacaaggtt tgcacaaccg tggctctttt gaaggattta
     2161 aacaagctgc atctgaagaa aaatggctat atgtgcatgg acgtaaagag tgggaaagtt
     2221 actacgctag agaaaatctc gaacgccaaa aatcattctt tgatttttac cttaaagaag
     2281 aaaataacga ttggaaagat acgcctcatg tcatttatga agttagagat caattttata
     2341 aaggcgaatt caaatcagcg tcagcgttcc ctttacctaa cgcagaatat acaccattgt
     2401 atttgaatgc tgaaaatcac acattgaatc atgcaaagat tagtagcgcg catgtcgcac
     2461 aatatgactc tgaagataaa caacaagatg taagttttaa atatacgttt gacaaagata
     2521 ctgagttagt tggaaacatg aacttaaaac tatgggtaag cactaaagac tcagatgata
     2581 tggatttatt tgcaggtatt aaaaagttag atcgtcgtgg taatgaagtt aacttccctg
     2641 attttaatca tattgaaaat ggtcaagtag ctactggttg gttacgcgta tcacatcgtg
     2701 aattagatca agaaaaatcc tcaatcgcgc aaccttggca taaacatgaa acagaattaa
     2761 agttgtcaca agatgagatt gtacctgttg aaatcgaatt gttaccttca ggcacgctat
     2821 ttaaacaagg cgaaacattg gaagttgttg taaagggtag tgaaattgta attggtaata
     2881 gtactcctgg catgaaaaca cgttatgaac atgaagaaac cgtaaataaa ggcatgcaca
     2941 tgatttatac tggtggtaaa tatgattcac aattaatcat tcctatcgtt aattgatatg
     3001 cagcaattac ggtcgctttt gattaaaagt gacatagtga taggactgta taacaagaga
     3061 aagccacacg cttggaatct taaaccaagg tgtggccctt tttattattg atggctattt
     3121 aattttataa cactatcgta ttttcttttt catgaatcat ttcaataatg acattatctt
     3181 cattcattac tgctacttta ggtgcatggt ttttaatttc ttcttcattc aactgtgcat
     3241 aagtcatgat tatgactaca tcgcctactt caacaagtct tgacgctgca ccgtttaaac
     3301 aaattttacc actacctctt tcaccagcta ttacgtatgt ttcaaaacgt gcaccattat
     3361 tattattcac gatggctact ttttcatttg gcaagatgtc taccgcttcc aatatatctg
     3421 aatcaatcgt aatgctacct acataattta aatttgactc agtcactctt gctctatgaa
     3481 ttttagcatt catcattgtt cttatcactt tattcagctc caattattat attatctatt
     3541 aaacgcgctt ttgaaaattt aacagctaac gagataaata tgcgtccagt tatttcgtgt
     3601 tgttctacta attgaggata actataaaca gcaacttctt caatgcgtcc acttatatgt
     3661 gattcaagat attcagtaac cctgtctata attactttac tttgacgttc accgtcttga
     3721 tacaacgctt gtgctaatag caaactttta cttaaatgta ccgcttcttg tcgttcttgc
     3781 tccgttaaat aaacatttct tgaacttttc gccaaaccat ctgcttctcg aacgatatca
     3841 ataccaataa tttcaacggc atgattgaag tcttttacca tttgctcgac aatagccaat
     3901 tgctgggcat cttttttacc aaaataagca taatccggca taacaatatt aaatagctta
     3961 ttaactactg ttaccacccc atcaaaatgc cctggacgct tcgctccttc taacacatca
     4021 gctaatgggc ctactttgac atcaatacct aattcacctg gatacatatc ttctactgca
     4081 ggatgaaaaa caatgtccgc tcctacttct gatactaatt ctaaatcttt atcaatttgt
     4141 ctcggataag catcgaaatc ttcgtttgga ccaaattgta atggattaac aaatacactc
     4201 acaattgtaa tatcatttgt actaactgat tcgcgtacca tcgttaaatg tccatcatgt
     4261 aaggcaccca ttgttgggat aaaaccaatc gttgtgcctg agcgtttggc tgctttaaca
     4321 atgtgttgca tctcttttac cgtagtaatc agcttagtca ttgttattaa cctcattcat
     4381 aattttcttt ttatacgtat attcttctga tggaaatgca ccagatttaa cttcttgatc
     4441 gtattgtttt aaaccatcca caccaacact aaaatcagca aattgcttca caaatttcgc
     4501 tttatgttca acaccataat ttaacatatc gtgataaacc aatacttgac catctgtacc
     4561 ttttcctgca ccaataccaa tgactggaat tgttaagtgc ttgctaattt cttctgctaa
     4621 atcatttgga attgcttcaa gtactaacgc aactgcacca gcttgttcta catttttcgc
     4681 gtctaaaata agttgctccg ctgcttcttt cgttgcacct tgtaatttat accccataac
     4741 gccaacactt tgaggtgtta atcctaaatg tgcaacaaca ggaataccaa ttgccgttgc
     4801 tttttcaata aatggtgtaa tatgcgctcc ttctgcttta attgcatttg cattcgtctc
     4861 ctgataaagc tttagagcat gatttaagtc ttgtgtcata gagatgccta ctgcaccaat
     4921 cggcatatca acaactacaa atgtatttgg tgcgcctctt cttactgcac gaccgtgatg
     4981 aatcatatct gctaacgtca cttgtacggt actttcataa cctaatacag tcataccaag
     5041 tgaatcccca acaagaatca tatcaatacc cgctgcttcc acttgtttag cacttggaaa
     5101 atcataagct gttaccatag aaattttagt ttgcttttgt ttcatatcta ttaattgact
     5161 tactgttttc aatgttattc aacctctttt tgcagtatta ttagaaatag tataatgaat
     5221 cttttataga aaggaaagaa gaagatgtta tcagttgcga ttatcggccc aggcgctgtc
     5281 ggtacaacta ttgcctatga attacaacaa tcattgcctc atacaacgct tatcgggaga
     5341 cacgccaaaa caataacata ttatactgta ccacatgcac ctgcacaaga tattgttgtg
     5401 aaaggttatg aagatgtcac aaacacattt gatgttatta ttattgcagt caaaacacat
     5461 caacttgatg cagttattcc tcatttaact catcttgcac atgaggacac gctcatcatt
     5521 ttagcccaaa atggttatgg tcaactcgaa catatttcat ttaaaaacgt atgccaagca
     5581 gttgtctata taagtggtca aaagaaaggc gatgttgtta cgcacttcag agattatcaa
     5641 ttacgtatac aagataatgc attaactcga caatttagag atttggtcca agacagtcaa
     5701 atcgacattg tattagaagc aaatattcaa caagctattt ggtataaatt gttagttaac
     5761 ttgggcatta attctatcac agcactcggt agacagaccg ttgcaatcat gcataatcct
     5821 gaaatacgta cactgtgtag acagctatta ctagatggtt gtcgtgttgc acaggcagag
     5881 gggttaaact tttcagaaca aactgtcgat actattatga ctatatatca aggttatccc
     5941 gacgaaatgg gaacgagcat gtattacgac attgcgcatc agcaacctct tgaagtcgaa
     6001 gcgattcaag gctttattta tcgacgtgca cgtgaacaca atcttgacac cccatatctt
     6061 gatactattt atagcttttt acgcgcgtat caacaaaata tgtaagcaca tatataatat
     6121 aaaaagacct taacgatgca tttagaacca cctgttccta atatcgctaa ggtcttttaa
     6181 ttatattaga ctattcagct tctctaattt cttctgccac atctttatag tctattttcg
     6241 ctttaacaaa cgtctcgtta tttaccggga aatgttgttg gaatgtttca aagttttgta
     6301 tctcaacgac aacgtcatcc acttcaaagt caagaacatg tccaccataa gctctttcat
     6361 catccgcaaa atgtatatga aaaccagcag accctacgcc atgaaataat tctggtgtaa
     6421 aaaatccaac aatggcacca cgaatatctt gtcttttttc ctcaggttgt ctgcgtgctg
     6481 aatcaatcaa acgtgtatat ggcggttgct gagcaggcat cattcgtaca tgcatatgtt
     6541 taaatgtgcc ataaatttta acagccgaaa ataaattctc acttaacatt tcatttttaa
     6601 tttgtgcaaa tacatcatct tgtgataatt gttgcaatgg aaatgtctta ctcgctttaa
     6661 aattagtaat cgatgcatac ggtactttct catcgccttt taattctata aattctttat
     6721 gctcgttagc atgatatgcc tttccgtcta aaaatattac ttcgccatca gaccctgtta
     6781 acgttgcaat ccctaaattc ccatgttcta acaattcatt aattgtagct gtgccttcta
     6841 gtaagccagc cattaacgta cctaatgtac catgttgata caagacattc gtcattccaa
     6901 aaccatctcc ctatagttat tcctcgttgt catgctctac ttcgtgatta atgcagaact
     6961 actttccttt atactcaatg acgcctcaac atttttattc atgtcactaa attttcacga
     7021 gtcataatac tttattcaat tattatttta cgcaaaagta ttgcctctat caatgcttgc
     7081 taaaaaaatg gacctatcag tgccacgtgt tactgcaaca ctaatagatc ctcttaccat
     7141 taaaatattt ctaattaata cttaaaatag gcttagtctt ctaataaata tttaattgaa
     7201 tcaaatgtat cttctaatgt tttaacagat ttatcgaaca acgcttgttc ttcggcactt
     7261 aatggcattt cataaatttt aactgcgcca tgttggttaa ctaatgttgg tacacctagg
     7321 taaacgcctt tgtgaccacc atattgacca tctaattgta tagaaacatt taagacatta
     7381 ttttcattat ttaaaatggc ttttgaaatg cgcatcaatg ctaatgcaat accatagtat
     7441 gttgaccctt tagcttggat aatttcataa gcagcgtcac gtgtattcac ataaatttct
     7501 tccgctttag cttcgctacc agtttgttct tttaatgtgt catatactga aatacctgct
     7561 acatttgctt gtgaccaaac tgcaagttca gtatcaccat gctcgccaat aatactagcg
     7621 tcaacacttg aaggtgcaac accaagttct tggctaatta aatattgtaa acgtgcactg
     7681 tccaatacag tacctgaacc gataacacgc tctgctggta atccagtata ttcttttaca
     7741 aatcttgtta aaatgtctac agggtttgcc gcgattaaga aatatccatc aaagccacta
     7801 tccataacac tcttaacgat gctcttcata atcttagtat ttttttcaac taattgtaaa
     7861 cgtgtttcac ctggcttttg aggtgcacca gctgtaataa caactaaatc tgcatcttta
     7921 cagtcttcgt attcacctgc tttcacatca actggtgaag gactgtggac tgtaccatgg
     7981 tttaaatctt gaacatctgc ttttactttg tcttttgcaa tgtcaataat tacaaattca
     8041 tcagcaacac cttgcgtaac cattgcaaag gcatagcttg atcctacaga tccatctccg
     8101 attaatacaa cctttttacc aaatgttttc ataattatgt ctcctaacct aattgaattt
     8161 attttacacg tgactacacg taaaaatatc tatactgaaa ttaaagatta acaattttat
     8221 tttattataa tattatgcta ccaaattaca aacataaaac tgagatgtaa gcggagtcaa
     8281 attacactct atcgccaata aatattgata tctcaagctt tagcacgtaa tttgaaattt
     8341 gtgacaattt ttagcattct aaaattgcgt aacgacgcta tttaaaggct ttaacaaact
     8401 attgagcttt ctattttttt cattttaaaa aggatggcta ttcgacttaa atgacttact
     8461 atataacctc ctattttccg ccttaccctc atcatatttg atgcatcatt ttatagtcaa
     8521 agaattacat ttcatacaaa ctagccaatc agacataaac atgaaattga ttttaaaaat
     8581 ataatgtgca aaattgttag aaaattagca aaaaatagta cttattcatt taatatctgt
     8641 tataatcagt tttatcattt aaaaaatcta tggatgttat caagacaagt cattaatacg
     8701 taacataaag ttgtgatttt tcagaatttt tagacaaaat attgattatt tgtgctatga
     8761 tataacatat taaaaaacac cgtatagaat tgtttatgaa tgtttgggag gaaagtttat
     8821 gggaagtttt ttcaataaaa tagcacgaaa agaggatccg gctatctatc aaaataaaga
     8881 tggtcattta aagcgtacac ttcgggtgcg tgatttctta gctttaggtg taggaacaat
     8941 tgtatcgaca tctatcttta cgctacctgg cattgttgct gcagaacatg caggaccggc
     9001 cgttgcgtta tcattcttac tcgctgctat tgttgctggt ttagttgcat ttacttatgc
     9061 agaaatggct gccgctatgc catttgcagg ttcagcctat tcttgggtca atgtattatt
     9121 tggtgaattt tttggatggg ttgccggttg ggctctatta gctgaatatt ttatcgccgt
     9181 agcctttgtt gcatcaggat tctcagcgaa tttacgcgga cttgtgaaac caattggcat
     9241 cgaattacct gcagcattat caaatccatt tggtacaaat ggcggtttta tcgatattat
     9301 tgctgctatc gttattttat taactgcatt attactatca cgtggtatgt cggaagcagc
     9361 tcgtatggaa aatattttag ttattttaaa agtattagct attattttat ttgtcatcgt
     9421 aggtttaaca gcaataaatg ttagtaacta tgtgccattt attccagaac acaaagtaac
     9481 tgctacaggt gactttggtg gatggcaagg catatatgct ggtgtttcaa tgattttctt
     9541 agcgtatatc ggtttcgatt ctatcgcagc aaactcagca gaagcacttg atcctcaaaa
     9601 gacaatgcct agaggtattc ttggttcttt aagcgttgct atcgtattat ttattgctgt
     9661 agcacttgtg ttagttggta tgttccatta ctcacaatac gcaaacaatg ctgaacctgt
     9721 tggttgggct ttacgtcaaa gtggtcatgg tgttgtagca gctattgttc aagctatctc
     9781 tgttatcggt atgtttacag cattaattgg tatgatgtta gcaggctcac gtttacttta
     9841 ttcatttgga cgtgacggct tattaccttc atggttaagc cacttaaacg acaaacattt
     9901 acctaatcgc gcactagtta tacttactat tattggtgtt ttaattggtt ctatgttccc
     9961 attcgcattt ttagcacaat taatttcagc aggtacactt gttgcattta tgttcgtttc
    10021 attagcaatg tatcgtttga gaaagcgtga aggtaaagat ctaccaattc ctgcatttaa
    10081 attaccttta tatcctgtgt taccagcaat tacatttgtt ttagtattgc tagtattctg
    10141 gggattagga ttcgaagcga aattatacac tttaatttgg tttattgttg gtatcatcct
    10201 atatttaagt tatggactta gacattctaa gaaaaatgac gtagctgaat accacccacc
    10261 aaaataaaaa ataaaaaaaa tcatttccca tctgattaac atccagatgt ggaaatgatt
    10321 ttttatttta tattaagata atcgctatta ccaaccttgt ccagatatgt catattgatc
    10381 taagttacct gcttccaaag cagtcagtgc atcttctata gtgtttaacg ccgtgtctaa
    10441 ttgctcatat gttattacca atggcggttg gaatcgcaac acatttcctg ctacagctat
    10501 aataactacg ccatgctcaa agcagtaatt acaaatttta agtgccgcac tggcatcacg
    10561 tgttttgagt tttttgtcgg aaacaatatc aataccaatg ctcagacctt tacctctaac
    10621 atcgcctaca ctattgtatt tagataccca ttgatccatt cgtttcctaa catattcccc
    10681 tttttccgca ctagcctgaa gaagcgactg atcttcaatc atttgaattg tggctaatgc
    10741 agcttcacaa ctaactggat tagcacctgt tgtaaataaa tgtgctggtg cttctaaaca
    10801 attcataatc tctttgcgtc caacaattgc tgacataggc ataccacctg ctaaggattt
    10861 tccgaaagtg attaaatcag gcgtaaaatt aaaatgcgag actgaactcc atgtacctgt
    10921 tctcccaaaa ccttgttgaa tatcatcgac agcgattaaa ataccatgtt cacgacaaat
    10981 cttttctaac gcttcaaaat accctggaac tggttctaaa agtccaccat cgccttgtat
    11041 cgtttcaata acaatacatg ctacttcgtc agcaggtaca tacttcgcaa acatttcttt
    11101 taagggtgct aaatattctt ctactgaatt agcttgtggc tgctcgtaca taccacgata
    11161 tttatctgga aacggaatat gataaaaacc attcaataac ggaccataat gtttgcgcat
    11221 atttaaacta atagctgaca tagacaatga gccaaaagtt gaaccatgat atgcattagt
    11281 gaaactaatg atataaggac gccctgtata tgctctggca aatttaatga tgccatcatt
    11341 tgcgtctgat ccggttaatc cgaaggtcac tcttttttca aaatctccag gtgcaatctc
    11401 acaaagcttc ttagctaaac gtaccaacgg ttcatgatac atatatgctg gtgtataatg
    11461 aataaattta tcaacttgtg cctttatcgc ttctgttact tctctaggtg catgacctac
    11521 gttttgagaa ctcgcactcg ataacaaatc gatatatgtc ttcccctcaa tatcaaccaa
    11581 tgttgctcca tatccatgat caatcactaa cggataatat ttaatacgtc ctgatttcgc
    11641 aaaataatgt tcatcctctt gaattaattg atgtgcttta ctcatataca ttcccctccc
    11701 attgacagta ttaatgtgca agcaaagtgg cctctcttaa atcgattata aaatccgatt
    11761 taaaatcctg ctcaaatacc gtcatgcctc tatgaatact atccaatata ctaataaaat
    11821 tcagatttgt aaatatattc tgataattaa gaattttata tcaatctact ttgtttatgc
    11881 gcataatttt gttcgattag ggaattgttt actatattcc atttgactct gaggtgatac
    11941 gaatgcgttt aaaagtattg ttccatttca ttgctgctat tttcatatca ttcatgttgc
    12001 tatggatgac catgttgttt gacttaataa gcaatcaatc acatttaaaa gctttattat
    12061 tgaatttaga ttttctaatt ccttctgaca atacaccata tattctagaa ataatatgtc
    12121 acttactaat tggtagcgtc atttattttg tatttgtgtt gctgtttcat acttctaaaa
    12181 ggctttacta tctatgttat atccctttat ttttcttatt tatagcattg tatccctttt
    12241 tagtatttat tgcgcaacgt ccaatttttc aatttagtgt gacagaatta atcggttgga
    12301 ttattacaca tattttcttt atgtcattaa tggcgttagt catacctaga ataaaataat
    12361 atttttaatt aaactgacaa ttatttttca cattttatac acttttcaag gtttatgact
    12421 tgagaaatgt tctcattttt atcatactgg aggtgtattg aaaagttaag gagtggaaag
    12481 tatgaataaa gagcaattag aaaaaatgaa aaatggaaaa ggctttattg ccgcattaga
    12541 ccaaagtggt ggtagtacac caaaagcact taaagaatat ggtgtaaacg aagatcaata
    12601 tagcaatgaa gacgaaatgt tccaacttgt tcacgatatg cgtacacgtg tggtaacttc
    12661 accttcattc tcaccagata aaattttggg tgctattctt ttcgaacaaa caatggatcg
    12721 cgaagtagaa agcaaataca ctgcagatta cttagctgat aaaggtgttg ttccgttctt
    12781 aaaagtagac aaaggtcttg ctgaagagca aaatggtgtt caattaatga aaccaatcga
    12841 caacttagac aatttattag accgtgcaaa cgaacgtcac atttttggta caaaaatgcg
    12901 ttctaacatt ttagaattaa atgagcaagg tatcaaagac gttgttgaac aacaatttga
    12961 agttgctaaa caaattattg ctaaaggttt agttccaatt atcgaaccag aagttaatat
    13021 taatgcaaaa gacaaagctg aaattgaaaa agtattaaaa gctgaactta aaaaaggttt
    13081 agatagctta aatgctgatc aattagtaat gttgaaatta acaattccta ctgaaccaaa
    13141 cttatacaaa gagttagctg aacatcctaa tgttgttcgt gtcgttgtat tatcaggtgg
    13201 ttacagcaga gaaaaagcaa atgaattact taaagataac gctgaactta tcgcaagttt
    13261 ctcacgtgca ttagctagtg atttaagagc tgatcaatct aaagaagaat tcgataaagc
    13321 tttaggtgat gctgtagaat caatctacga cgcatctgta aacaaaaact aatttataca
    13381 ttaaactatt ttttgatata aactactaaa aaggactcat acatgtgctt taaaggcatt
    13441 gtgtatgagt cttttttgat taaagttgat tttgccgtat gtattagatt acgtaaaaaa
    13501 atcggacagc tgaaaaatct ttaagatttc tcagttgtcc gattttaaat atagctatta
    13561 gaatttagtt ttcgtagtaa cctaattcta agtcttttga agtttgttta cggatttttc
    13621 tcattaattt ttcgtcttca attaatgatt caccgtatga tggaatcatt ttcttaattt
    13681 taggtgccca ttcagttttg tattcaggga agttacgttc taatacttct aacgcaactg
    13741 aaactgaagt tgaagcccct ggtgattcac ctaataatgc aattacagtg tggtcttgtg
    13801 agttaaccac ttctgtaccg aattggatga atcctttacc gtgttcaggt gtatctttga
    13861 taacttgtac acgtttacca gcagtgtata attgccaatc ttcattacgt gcttctggat
    13921 agaaagtacg taagtggttc atacaacctt cttttgtcat aattacttgg tcaaatgagt
    13981 atttaattaa aggtaagttt ttaactgctg ctgctaataa agttgtaatg ttgtatgttt
    14041 taacagactt gaataaatct aagttagaac catttttcaa gaatttaggt ccaacattag
    14101 caaatggtcc aaataataat gttctttgac catcaatgta acgcgtatct aaatgaggta
    14161 cagtcattgg tggtgtacca ggtggctctt taccataaac tttggcatcg tgttgttcaa
    14221 taacttgtgg gtttgtacaa gctaagaatt gaccactgat agggaatcca cccaaatgtt
    14281 tactttcagg gatacctgtt ttttgtaata atggaattgc tccaccgcca gcaccgatga
    14341 atacgtagtc agttacttgt ttgaatttct caccagttag gcgattttta acagtaactt
    14401 cccattgacc atttgataat tgttcaaaat caacaacttc atggttaaat tgcactgtag
    14461 catttggatg tgcttcaatg cttttagcca ttttacgtgt taattcaccg aagtttacat
    14521 ctgtaccttc gtcaatttta cttgccgcca tgataccagg gttatcttca cggcctttca
    14581 tcatcaatgg aatccatttt ttcattactt cgatgtcttc agtatattcg atattatcga
    14641 acatagggaa agctttcatc gcttcgtaac gatcttttaa gaatttaaca ttgtttttac
    14701 ctctaacata actgatgtgt ggtaatggat tgataaattc tcttgggttc tcgatgctac
    14761 cgcttttcac taagtgaccc cagaattgtt ttgaaatctc aaactcttcg ttaatcactt
    14821 tcgctttttc gatgtcgata gaaccatcag gttgtaaaac tgtgtagttc aactcacata
    14881 atgctgcatg acccgtacca gcattatttc tttcgtttga actttcgatt gcaggacgat
    14941 ccaagcgttc gtaaacgtgg atattccagt ctggctcaat ttcttttaac attgaaccaa
    15001 atgttgtgct aagtacaccg gctccaatta aaacgatgtc tttactatta gacttagcca
    15061 ttggtttcac ctctccaaaa attgtaaatg tgtatcatca atatgaaagt tacataaaac
    15121 tgacatattt ctttaaaata tcaacgccat tgataacttc ctgttttaat tgatacgctg
    15181 taacaaaata ctatagttag tgcttacatg tatatgttaa agcaagcagt ggtaaatgta
    15241 aattataatt attcattaac tttgcaatat attaaatctt ttattcatag aagataaata
    15301 tcaaatcaat cataattatt tgacaacaaa tagctaacga ttgttttaat ctacatttgg
    15361 cttatagcat tttaaaccta tactctattt tgatacaata taagtgtaaa atcaatcata
    15421 aaaaggatat tcaatatctg catccaagaa aaacattaca attaccttta atattgaaaa
    15481 ttattttgaa tcttgcactt ataggtattg taattatcgt tctaacacat actgagtttc
    15541 aacttaatgc cggtgatagt aatgtgtttt tattaaactg ggatattcga ggtttaacat
    15601 cttatatttt ctcattctta atactggttg cgcttgtttc taaactaatt ttaatattta
    15661 taactagacg acaaagaaca tcataaataa acaagcagtc cagtgcgtat catcatacct
    15721 tacgcattgg actgctttgc tatgtttatt gattgctatc tttttgttgt tgccattttt
    15781 ctatttcagc atctcgtagt tcaacacgac gaattttacc tgagtttgtt tttggtaagt
    15841 cgtcaacgaa ttcaatctct ctcggatatt tatatggtgc aacttcattt ttaacaaatt
    15901 gttgtagttc tttaactaac gtatcatcac ccgcagtatg gtcctgtaaa ataacgaatg
    15961 ctttaacaat atttcctcgt atatcatgag gttttgctac tacagcacat tctttaacgg
    16021 ctggatgatt cgttaacgca tcttcaactt caaaaggccc aatcgtatag cctgaactaa
    16081 taataatgtc atctcgacgt ccttcaaacc agaaataacc atcatcatct acatgagcta
    16141 agtcaccagt gatgtagtat ttacctgttt gcgctttcgc cgtacgttct ggctctttat
    16201 aatacccttt gaaaagtgct ggcaaatcaa gtggtactgc aatattccct ttcgtattag
    16261 caggtacgct attcccctca tcatctacta cagtgaccga actacccgga atgcctttac
    16321 ccattgatcc aatcctctgt ggtgtatctt ttaaaaagcc tataagcaag gtactttcag
    16381 tctggccata tccatctctt acagttaaat taaagtattt cttgaattgt tcaactactt
    16441 ctcgatttag tggctcacct gcagaaacgg cactatgtaa atgcgttaag tcataatcat
    16501 ttaagttctg taatttagcc atcatacgat attctgtcgg tgtacaacat aaaacattaa
    16561 tttgatattt ttgaagcaat tctaagtatg tttcaggact gaaccttcca ttaaatacaa
    16621 aagcagttgc acctgaacct aatacagata agaaaggact ccatacccat ttttgccaac
    16681 ctggtgctgc tgttgcccaa actaagtcat cttcattaat acataaccaa tgttttggtg
    16741 ccatttgtaa atgtgcaaat ccccatccat gacaatgtgt aacggcttta ggattgccag
    16801 ttgtaccaga tgtatatgac agaatcgcca tatcatcacg cgtcgtatct gccatttcta
    16861 gtttgttact tgcgttttct ttttcagctt caagtgaaat ccatccatct ttttgaccgg
    16921 caataacaaa tttagttaac gcatcatatt ctttaatttt ttcaaattca actgtgaatg
    16981 gctctagtgc aataactgca ttaatttcac catgtgtgat acggtattgt aaatctttag
    17041 ttcttagcat ttcagaacat ggaatgattg caacacctaa ttttaaagca gcaatatata
    17101 attcatacgt cgcaatagat cgtggcatca taatgagtac tttatcgcct ttagataaac
    17161 cgtgcgatgc taaaacatta cctactttat tagactgttc aatgagctgt tggtaagtga
    17221 ctgatatatc ttcgccttca gtattatgat ataaaattgc ctttttatct ggtatgtggc
    17281 tatatttttc gatttccgaa ataatgttat atttttcagg cgcgaataga gctgactttt
    17341 gcataactaa cttcctttca tacatccact tttcctgtga tgaacattgt aattttataa
    17401 atgaattata tacatcatac gcctatcttt acagaatttt caattaaata gggttaaata
    17461 ccaaagtcct cgacactaca ctttgacatg acgtagcatt caaggacttt caaatgattg
    17521 agggttgata tctcgggcta gaccatatca gctaattcaa tacgaatatt gtatgataat
    17581 tcacgattaa ttatttttac atctgcacct ttcgaagtgc cacgatgctt gtgtgtatgc
    17641 ttgtactcag ctgaattttg ccaatgataa aatgcttgcc tattttccca cagcgtaata
    17701 atgatatagt gtctaccagc tgttctaggt cttaaaaacc ttaatgcttt aaatccatca
    17761 acgtttttta aatgcttatt acgtcgcaaa aatttttgct cgaatgtttc ttcttgtcct
    17821 tcgttaatat ataaatgatt taacacacat aatgaatcat ttgataaatc atttatcgct
    17881 tctagcacat cgtaatatgc agtgtcatta tttttttgta ttgtgagaca atcatccagt
    17941 tcttctatta cataacttct ata
//
LOCUS       DAKTIP010000033        17950 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAKTIP010000033 DAKTIP010000000
VERSION     DAKTIP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.033"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            236..940
                     /locus_tag="O0305_000889"
     CDS             236..940
                     /locus_tag="O0305_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0274043.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1333..1869
                     /locus_tag="O0305_000890"
     CDS             1333..1869
                     /locus_tag="O0305_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274044.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
     gene            complement(1982..3523)
                     /gene="guaA"
                     /locus_tag="O0305_000891"
     CDS             complement(1982..3523)
                     /gene="guaA"
                     /locus_tag="O0305_000891"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCY0274045.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3548..5014)
                     /gene="guaB"
                     /locus_tag="O0305_000892"
     CDS             complement(3548..5014)
                     /gene="guaB"
                     /locus_tag="O0305_000892"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCY0274046.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5052..6320)
                     /locus_tag="O0305_000893"
     CDS             complement(5052..6320)
                     /locus_tag="O0305_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCY0274047.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6320..6898)
                     /gene="xpt"
                     /locus_tag="O0305_000894"
     CDS             complement(6320..6898)
                     /gene="xpt"
                     /locus_tag="O0305_000894"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCY0274048.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7129..7231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7411..7818
                     /locus_tag="O0305_000895"
     CDS             7411..7818
                     /locus_tag="O0305_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCY0274049.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7961..8623
                     /locus_tag="O0305_000896"
     CDS             7961..8623
                     /locus_tag="O0305_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274050.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8741..9697
                     /locus_tag="O0305_000897"
     CDS             8741..9697
                     /locus_tag="O0305_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274051.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKALAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKAENAGDGTAT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
     gene            complement(10488..10589)
                     /locus_tag="O0305_000898"
     CDS             complement(10488..10589)
                     /locus_tag="O0305_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274052.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNSVVIGGKGK"
     gene            10674..12062
                     /locus_tag="O0305_000899"
     CDS             10674..12062
                     /locus_tag="O0305_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCY0274053.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLIYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(12143..12898)
                     /locus_tag="O0305_000900"
     CDS             complement(12143..12898)
                     /locus_tag="O0305_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCY0274054.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            13390..13959
                     /gene="ahpC"
                     /locus_tag="O0305_000901"
     CDS             13390..13959
                     /gene="ahpC"
                     /locus_tag="O0305_000901"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCY0274055.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            13975..15498
                     /gene="ahpF"
                     /locus_tag="O0305_000902"
     CDS             13975..15498
                     /gene="ahpF"
                     /locus_tag="O0305_000902"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCY0274056.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEIT
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            15628..15870
                     /locus_tag="O0305_000903"
                     /pseudo
     CDS             15628..15870
                     /locus_tag="O0305_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15943..16569
                     /locus_tag="O0305_000904"
     CDS             15943..16569
                     /locus_tag="O0305_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCY0274057.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            16824..17207
                     /locus_tag="O0305_000905"
     CDS             16824..17207
                     /locus_tag="O0305_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274058.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSLTQFTPFTLASFIT
                     SIILFTSTIITLAIGGKSVEKNDSP"
     gene            complement(17273..17854)
                     /locus_tag="O0305_000906"
     CDS             complement(17273..17854)
                     /locus_tag="O0305_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0274059.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYDKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNKVSRAEAVDLKVENCKPYIIE"
BASE COUNT         6084 a         3311 c         2817 g         5738 t
ORIGIN      
        1 taggaattcg cttataatta gatgttcaat atagactttt ttacacatac atgaactata
       61 aaaagcgaat aaatagcagg tcattgcgaa atttgctaaa tacaaaattg atgagttcca
      121 cctaataaaa attgttggtt ttatagtaac atctattttc gtaccattca tgttaatttc
      181 agtgctaata atgctgatat aaagaagaat aaattgatta ggagaacact atttaatgtc
      241 taaaaatatc aatcaagcaa atagtaaatt aaatacgtca aacaaaaaac taaaacaagc
      301 ttattcaaaa tccgacagta aaaacatgaa agttatatac atgcctcatt ggctagagtt
      361 ttattcaatt gctatacata gcgatgttac aagcaataaa aaaagatatt ataaatcata
      421 tagtagagga acagtggtat atgtaaaact agggagtaat attggaagtg agttttctgg
      481 taatcatttt tgtgtaatat tagataacaa agacaataaa ggaaaagaaa cagttacaat
      541 agttccactt tcttccaaag gtaataaaaa ttatttgaag ttaaacgaat cggtgctaaa
      601 tctaacagca acagatttaa aaaaacagat tatcgatatc agtagtaaag tacaagcatt
      661 agcccaaaaa tatagagaaa ttgacattaa acttagcaca gaagataaaa agcttttgtc
      721 taacttaaat caaaaagcta atgaactgta tagagtgata ggtgtttatt ccaagcacaa
      781 agggaaagat acatacgtta atatatcagc aattacaaca ataagcaaaa gacgtataag
      841 caaaataaat gattctgatc ctactggaac gataataatt tcagaagatg acatgaatga
      901 aatcgaaaag caattaaaga taaagttctt ttctaattga attgtaatat tacatctgat
      961 ataattgatt tacaaattcc cggtaaccat tccggcttca ttctcttgaa gaatgacata
     1021 ttctcagcgt tttagctgaa ggtcagatga tacgtcatct ggcctctttt tttatataaa
     1081 tgccctttaa gtgaaatata caacaatctg caatcaatat tagacatcac aaaacacaca
     1141 ataaattaca caatgtgtta taagccacat gtcactatta tcgcagctag ctgcgtcact
     1201 caaaacgaat acggaacgcg ttggttattt caatcataat attactctgc aaatacacag
     1261 ccctgtaacc gagtaaatgg atagagattt gaacaaatga aaacaatcaa ctaatggaaa
     1321 ggataaaata ttatgcacaa caataatgaa ttcaacaaaa aactgaaaga ttttataggt
     1381 agcgataaac ggatggcttt agtaaagggt tatgttaacg agtataaatt aacaactgtt
     1441 ttaagagctt taaataattc cgattacaca aaagggacga tacacactcg ttcaataggc
     1501 aacctaaaac aaatagtaga taatcgaatt attcctaaaa atataactca aaacacaatc
     1561 ttcaaattga gtaatttaac cttagaggtt aacctctatg aaagaaacac aatttatcac
     1621 ggagactttg ctatttacta tcctgtagaa tctgctctaa taatagaaac agatactcct
     1681 aggctaatta atcatataaa taacaaccca gtccaaaaaa tatttataat aacaacaaat
     1741 gactggagct ttaatacact tgaattagaa aaaatagtag acgaaactat aatttatgat
     1801 ttaaaacaag aagatcctaa acaatatgaa attttatata aaaataaaca cggaaaattg
     1861 ccttattgat gaattaaaac gcaaacttag aacaccttac cccttttccg tccttattgt
     1921 aattttccaa ctactcaaat taaaaaccac ccgtgcaaac aggtggtttt attatatata
     1981 attattccca ctcaattgtg cttggtggtt ttgatgtaat gtcatagact acgcggttga
     2041 cgtgatctac ttcgtttacg atacgactag aaatcttttg taagacttcc caatcgatgc
     2101 gtgcgaagtc acttgtcata ccgtcgatag atgttactgc acgaatacct actgtgtgat
     2161 catacgtacg gtagtctccc ataacaccta ctgattgaat gtttggtaac actgtgaagt
     2221 attgccaaat ttctctttca agaccttctt ctctaatcac ttggcgtaaa atcgcgtctg
     2281 attctctaac gatttctagt ttatcttcag taatttctcc aagtacacga ataccaagac
     2341 caggtcctgg gaatggttgt ctccatacta aatgttctgg aatacctaac tcaataccta
     2401 atttacgtac ttcatcttta aacaatgtat tgattggttc gattaattcg aattccatgt
     2461 cttctggtaa tccaccaaca ttgtggtgtg atttgattgt ttgtgctgtt ttagtacctg
     2521 attcgatgac gtctgtatat agtgttcctt gcgcaaggaa gtctacacct ttcagttttg
     2581 atgcttcatc atcaaatacg tatacaaatt cattaccaat gattttacgt ttttgttcag
     2641 gatctgaaac accttttaat ttattcataa agcgatcttt cgcattaaca cgaataatat
     2701 tcatgttgaa accttcaccg aattgctcca taaccatgtc gccttcacct ttacgaagta
     2761 agccatggtc tacaaagata catgttagtt gatcacctat tgctttatgc aatagtacag
     2821 ctacaacaga tgaatctacg ccgccactca tcgcacataa tacacgacgg tctcctacgc
     2881 gttgacgaat cttttcaatt tcgatttcga taaagttttc cattgtccat tgacctctac
     2941 aatcacaaac acgacggaca aaattattta ataaatcatt accatattct gtatgacgta
     3001 cttctggatg gaattgaaca ccataaatgc gacgtttctt atcttcgatt gctgcatagt
     3061 ctgtgcttgg gctatcagcg ataacttcaa agccttctgg aatttcaata actttatcag
     3121 aatgactcat ccaaacagtt tgttctgctg gtaagccagc gaataactca tctgacttcg
     3181 cattaatgat tgctttaccg tattcacgtt cattggcacg ttcaacttta ccacctaata
     3241 atttagtagt taattgcatg ccgtaacaaa taccaagtac tggaattcct aaattatata
     3301 tttccggatc aattgtaaat gaaccttctt cataaactga atttggacca cctgataaga
     3361 taataccttt tggattcatt ttcttaattt cttcaattga aatttcatga tcgtgtaatt
     3421 cactataaac gcccatttca cgaattcggc gtgtaattaa ttggttgtat tggctaccaa
     3481 agtctaagac aaggattaac tcttgttctt ttgccatttc catatttgtc gttctccttt
     3541 atcttaatta gaatgagtag ttcggtgatt ctttcgtaat ttgaatatta tgtggatggc
     3601 tttctgctaa accagcagga cccatacgtg taaattgtgc ttcttcgcgt aattctctta
     3661 aatcgtgtga accagtataa cccataccag cacgcacacc gcccattaat tggtaaattg
     3721 tatcttgtaa cgcaccttta taagccgtac gtccttcgat accttcagga acaaatttct
     3781 taggcgcttt gtcctcttgg aagtaacggt cgtttgaacc tttttccatc gcacctaaag
     3841 agcccatacc acggtatact ttatattgtc taccttggaa aatttctgtt gcgcctgggc
     3901 tttcttcagt acctgctaat aagctaccta acataaccgc atgtccacca gcagctaatg
     3961 ctttaatgat atctcctgag aatttaatac caccatcagc aatgatagct ttaccatgtt
     4021 tgcgtgcttc agttgcacaa tcataaattg ctgtaatttg tggtacacca acacctgcta
     4081 caacacgcgt cgtacaaatt gaacctgggc caataccaac tttaacaata tctgcacccg
     4141 cttcaaataa atcttttgtt gcttctgcag ttgctacgtt acctgctact aatgtgattt
     4201 ctgggtaagt cttcttaata tgtttcactt gatcgataac acctttagag tgaccatgtg
     4261 ctgtatcgat aactaagaca tccacacctg cttcgactaa tttttgagca cgaatatcag
     4321 tatcttttga aataccaatt gctgcggcta caagtagacg accatgttca tcttttgctg
     4381 cattagggaa ttcgataact ttttcaatat ctttaatagt aataagacct tctagacgtc
     4441 cgtctttaac taatggtaac ttttcaatct tatgtttttg gagaattttt tctgcttctt
     4501 caagtgttgt attcactgga gctgtaatta aattttcttg cgtcattaca tctacaattt
     4561 taatcgagaa gtcttcaata aaacgtaagt cacggtttgt taaaatacct actaagttgc
     4621 gatcttcttt attatcaaca attggtacac ctgaaatacg gtatttaccc attaatgctt
     4681 ctgcttcata aacgctttct tctggcgtta agaaaaatgg gtttgaaatg acaccatttt
     4741 ctgagcgttt tactttttga acttcgtccg cttgttcttc aacgcccata tttttatgaa
     4801 taacacctaa accaccttga cgagccatag caatcgccat tttagattca gttacagtat
     4861 ccataccagc agaaataact ggaatattta atttaacttt gtctgataat tgtacgctta
     4921 aatcaacgtc tttcggtaaa atatcagatt gtgctggaat taataacaca tcatcaaacg
     4981 ttaatgattc ttttgcaaat ttactttccc acattaaaaa cagcctccat ttttcaaatt
     5041 aattagttat attatttcac attttcttcc gtttgtttat actttattcc attaaaaaag
     5101 aaattaagaa taattgcaga gattgcgcct aaaacaattc cgttttgagt caaccatgca
     5161 aattgttcac ctaaaccttt aaatgcttgt ggtacagcgc ttataccagt acctaatcct
     5221 actgatacag cgataattaa taaattgttt tgatttttaa aatcgatatg tcctaatata
     5281 ctaacaccat atgccattac catgccaaac atagctatca tcgcaccgcc taacacaggt
     5341 agcggtatga tatttgctaa tgcgccaagc ttaggtatac aaccacatat aagtaataac
     5401 acgaccatgc cgtatataac attgtttttc ttagcgccgg ataaagaaac aagtcctaca
     5461 ttttgcgaat aggctgtata cggaaatgaa ttgaatatag aacctaacac tatcgctaga
     5521 ccttccgcag tataaccttt acgaaaatct tttctttcta acttcttacc ggtaatttca
     5581 cttaacgcat gatagacacc tgtcgactca attaaactaa cgatagctac aataaagaac
     5641 actaacgtcg atgtcacatc aaagctaaat ccagagaatc taaacggcac tgggatgcct
     5701 aaccaaccgg catgattgac ttgattaata tcgaccatcc caagtaagcc agcacctatc
     5761 gttcctaaaa cgagtccaat taatatggca atactcttaa taaatccagt tgtgaatctt
     5821 tgtaaaagaa gaataatgat taatgtcatt aaacctaaca aaatgttctt aacatctcca
     5881 tagtcctttg caccttgacc tccagctaag taattcattg ctactggcat taaattgata
     5941 ccaatgatag taacaacact acccgttact actggtggga agaattttac aagatgtgaa
     6001 aagaaaggcg cgatgataat aactaatatc cctgataaaa atagcgaacc ataaagtaca
     6061 tctattcctt tcgtttgacc aattaaaatc atgggcgcaa cagccgtgaa tgtacatcca
     6121 agaacgattg gtaatcctgt tcctgttact ttattggctt gtaaaaatgt ggcaacccca
     6181 cacataaata tatctactgt aactaagtaa gcgatttgtt caggtgtaaa cttcaaactt
     6241 gtaccaacaa tgattggaac taagatagca cctgcgtaca tagctaaaag atgttgaaca
     6301 cttaggatta aatttttcat tattcttctc ccaccaatgt cactttgttt ccttctagtg
     6361 aagcaacctt gcagagagaa gaaactgtta aacctgcttc ttctaaacgt tgatgcccat
     6421 tttggaaact cttttcaaca acaataccaa taccagctgt cttagcattc gcttgctgtg
     6481 cgatatcgta taatcctaat gaagcatcac catttgctaa aaagtcatcg atgataagta
     6541 cagtatcttc ttctgataaa aactcttttg aaacaatgac cgtacttgtt ttatttttag
     6601 taaatgaatg aatagatgtt tcataataac catccgtcaa agtgctaggt tttgcttttt
     6661 tcgcaaataa acatggcaca tcaaaatgca gtgcagccat gattgcaggt gcgataccgg
     6721 aagcttcaat ggttaagatt ttagtaatcc ctttatcttt aaattgctcg taaaaagtgc
     6781 gaccaacttc attcattaac tttgcatcaa tttgatgatt taaaaatcca tcgactttta
     6841 aaatcttctc atcaatgaca acgccgtctt cctttacttt ttgtcctagt aactccactc
     6901 ttaaaacctc ctcagatttg tgtgaaacat tttgctctta aattggtgct agatacaaaa
     6961 aaatccccaa actaaataat agtttcaggg tttatgagtg aacgaacatg cataacgaat
     7021 ttgtcatgca atcaatgtaa aagaagtttc atcaaataaa ctgtgaccat catataaaat
     7081 gatataaatc acccaccatg gttacaattt aatggctgaa gctactccta gtattgtgtt
     7141 gttactcata gtcatgtcgt tcaaggcaac atggtagaaa cttctaaagc catattcttt
     7201 agattatatg agtttatgta aattatttaa cgataatagc aaattttcgg cattttttca
     7261 ataactgctt aggtaatctt ttaatagttt tataaatgta cagagacgtt taatgcgaat
     7321 gaagacttat aacttaataa tgtaagggtt ttactttata ataagaaggg taaaagatta
     7381 aataaacaaa tttttaaagg ggcgacaact atggcagata ttactgtagt aaatgacact
     7441 ggcgaattat acaacgtaat caatcaaaag aaatctgaag gttatttaga atcagagcta
     7501 acaattatta gtaaaagtaa attacaccta aatgacttac acgactctga aatctccctt
     7561 atctcaacaa gtggaacatt tagtgataga atgactaaac tgttaacagg agaagatggt
     7621 gaacatgcag ttttatcacg ctacaactta gcgcctgatg aattagaaaa atataaacaa
     7681 cttattttag acgataaaat gttagttgta gcggttcgtg acaaatcatc acatcaagaa
     7741 gtgcatgaaa ataattcagc ttatgaagag attgatatta cacattttgc tgaagcatct
     7801 aaaggtccca aagcttagtt atcacactga attcaaaatg agattactgt actgtctaat
     7861 ttaagatagt acagtttttt ataccattta aaagtttatg aacagtcttt tacagcaggc
     7921 gattatgcta taatattatt taaattgggg aggtgttctt atggaattta aaaccattca
     7981 atctgtcaaa gatccacatt acaacgaagc acttaaactt tacgatacta agttaaattt
     8041 atctttaact gaagataaac gtatcatcaa acgctcatta gaaaacaaca aaaccgaaaa
     8101 cgattatgtc tttatcgtag gcttagaaaa cgatgttgtt gtcagtctag caactgcaca
     8161 ttatgaagcc acaaccaatt cagcattttt aatttattta ttcgcaaaag acctacccaa
     8221 ccatgatgag ttaatgacgc aaacattaca tcaaattgaa catgaactaa acttattatc
     8281 aaaccttgtt cattctagaa gtattaactt tttaatgatg gaagtcccta aagaacctgc
     8341 agatttaaca caagataacg gcctcattat tgaacaccgt agacagtttt tatacgaaca
     8401 tcaatttgaa agacaaactg aaattgatta cattcatcca actaatgatg gtcacactaa
     8461 aaaagtggac ttatttttaa agaaaaatat taaactgaca aaggatatct acggtacaag
     8521 tgttaaatca aattatattt tgaagtatgt atttgccaat ggtatcgcac gtgaaattat
     8581 ttatgcttta ttagaagaaa tggatttacg taaaccattt taaacaaact tgaaatcact
     8641 aacaataacg tttaaactat gtatgaagca agactgacgt ttcactgccc gtagacaaac
     8701 gtcagtatac ataaagtata tgcagaaagg actttaaagt atgttaacta aagaatttgc
     8761 acaacgcgta gaactaagtg agaagcaagt acgtaaaatt gttcaacatt tagaagaacg
     8821 tggttaccaa ctaagcaaga ctgaatatcg tggtcgtgaa gcaactgatt tcaaagaaga
     8881 agatatcgag cttttcaaag acattgccga caaagtaaaa caaacaaata gttatgatct
     8941 agcgtttgat gaattagaaa aagaaaaaga cttcctgcaa gtcattgtca aaaacgatga
     9001 caaaaactta cctactaatc aaaatgtcgc tcaactagta gaagatttac gcctagaaat
     9061 ccagaaaatg cgcgaagaac gtcacctact tggtcaaatg atgaatcaag tacatcagca
     9121 acaacaagaa ttaaaagaac ttcaaaatca acttacatct aaaatcgatt caaatagcga
     9181 atccttaaaa gccatccaaa catcacaaga ggctatccaa gaagcgcaag cctctcaagc
     9241 aaaagcatta gctgaatcaa caaataaagt tgaaaagaat gctgttactg aagataaagc
     9301 tgacagtaaa gattcgaagg ttgctggagt gaatacatca acagacgcta agacggatac
     9361 taaagcagaa aatgcaggcg atggcactgc tactaaagta gataaagagg atcaaatttc
     9421 tgcaactgaa gcaattgaaa aagccagcgt tgagcagtct aaaaatgaca atgcagctga
     9481 gacttcaaat aaagaagcta cagtagatgc agaagcgcaa catgatgctg aacaacaagt
     9541 agcagaagcc catgcagaag cttctaaaca agctacttcg aacgattcat tggaagcgaa
     9601 agcagaaaat gacagcacag cttcacagtc agaaatgtca gaaccaaaac ctcaagaaga
     9661 gaaaaaaggt ttcttcgcac gtttattcaa cctgtaaact tgtgatttga acacaaattt
     9721 aatactcaat tcatatatag ctttgattta aaaactgatt tttataaacc tatagaactg
     9781 taatttactt tcaaatttaa tactcaattc ataaaacctg aaacaagctg atatactcca
     9841 ttcaatatct actttgaaga gagcatatca aactaatttg tttcaggttt tttaattatt
     9901 cattatgatt aatattgaat tttgaataaa tttttatagt atgttacttt taaatgggat
     9961 ggcttctgtg tacttctaaa tagattcatc ttaataagtc cattcaattt aaatgtttgg
    10021 ctgtgttagt aactgacgaa tcgcacaagt gcacactcat attcacattt actacaaacg
    10081 cctttttgct caaacaaaaa tatttgttta cggtagcttt tgaactacca cctgcacaca
    10141 cgaacactca aaagatgcta attttgattc gacagaattt tttacaacgt gtttgttcga
    10201 aacgtctttt caaggttgat catttattat cttttcattc aataaatatg aggttagcaa
    10261 tttttatcaa accgccatag ccacatctcg attcaaggat aaatttgttt tctcatatct
    10321 tatctctcat gacagtgcac gatatatatc ttttccaaat attcatcact tttttcctgt
    10381 taaaatcatt tctattatta atgctttaat gacaacttat ttaatttatt gcatttaatg
    10441 ccctgcttca ttttcaaata ttaactttaa tcgactagct catcacacta tttacccttt
    10501 ccacctatca ctactgaatt caaaacgatt tgtttacaaa tccccttatg ccaacggtcg
    10561 aatttcatca tgtttacatt taattgcaaa tgccctcttg aaatatttta aaattaatat
    10621 tattcttagc atactagtcg gaattatatt caaaaatttg aggagtgacg ttcatgaatg
    10681 cttttcttac actgattaat attattgtat tggtgatttt catcgtaata ttgcacatga
    10741 tggctcgcaa acatatatct tttgcgaaac gtgtgtttac agctttaggg attggtatag
    10801 tattcggtgt gttattgcat cttatttatg gcacacattc gaatgttatt acatcaacaa
    10861 gtgactggtt caacattgtc ggacaaggtt acgttgcatt gttgcaaatg attgttatgc
    10921 ctttaatttt catttctatt gtagctgcat tcactaaaat tcaaatcgga gaaaagtttg
    10981 ctaaaattgg tagtctcatt tttatctttt taattggcac tgtcaccatt gctgcgattg
    11041 ttggtgttgt gtatgccttg gtgtttggtt tagatgcatc aacaattaac cttggtaacg
    11101 ctgaacaagc acgtggaagc gaaattgcta aacaagcgaa agatttaaca gcacatacgt
    11161 taccacaaca aattttggaa ttattgccta aaaatccatt cttagatttc actggacaac
    11221 gtgcaacttc cacgattgct gtcgtgatat ttgcttcgtt tataggattt gcttatttac
    11281 gcgtagcacg caaacaacct gatcatggtg aattgcttaa gcgtgcgatt gacgctatct
    11341 attcactagt aatggcgatt gtgacgttcg tcttacgttt aacgccatac ggtgtattag
    11401 caattatggc caacacatta tcgacaagtg actttggcgc catttggact ttaggaaaat
    11461 tcttaatcgc atcgtacgct gcattaatta cgatgtatat cattcactta attattttaa
    11521 gcctgttagg tattagccca attcgctatg ttaaaaagac tttggaagta ttaatctttg
    11581 cattcacatc acgctcaagc gcgggtgcct taccattgaa tgtacaaaca caaacaagac
    11641 gtctaggcgt gccagaaggt attgccaatt tcgctgcgac atttggattg tcgattggac
    11701 aaaatggttg tgcaggcatt taccctgcca tgcttgcaat tatggtcgca cccgtcgcta
    11761 atgtagaaat tgatttacaa tttattgtaa cgttaatcgc tgtcgtgatt attagttcat
    11821 tcggcgttgc tggcgtcggt ggcggggcta catttgcatc catattggtg ttgtcaacgt
    11881 taaacttgcc agtcgcttta gccggcgtgc tcatctccgt tgaaccattg attgacatgg
    11941 gtagaacagc attaaacgtg aacgattcaa tgctagctgg tacaggtacc gccaagttaa
    12001 ctaaacattg ggataaagat actttcgatt caaatgataa tgctgcatta acttcacact
    12061 aattcattgc aagaaatacc cgccccctct agctatactt atcattttaa gctagagggg
    12121 ggcgggttat tttggtatct tgctatcgct gtattaagcc tgatttttgc aattgttcta
    12181 acatatctaa tcttgctttg tttcctagga acatctcaat ttgctgcgac catgtttctt
    12241 tgcgattccc gtttgtacgt tgatcatagt actcgctgat tgtctggtcg taatctgcca
    12301 tttgtgcata ctgtgtttcc ttatcagcat gatacttatt atgatggaag acatggtcaa
    12361 atggtaagcg tggcttggct gcaccatttt cgtcatctgc gggttcccct actgccatac
    12421 caaataccgg gaagacatag tcaggtaagt ctaaaatttc tcgaacgcgt tcaacatcat
    12481 ttcttaatga tcctaaaaag acaatgccat accccatatc ttcagcagtt accgcaatat
    12541 tttcggcaac taatgctgca tcgattgcac ctactagcaa accttccgtt gaaccatatg
    12601 cattttccat atcagtttca gcatgttgat caactaaatg atgacgataa taatcaataa
    12661 caaagacgaa taaatagcca ttttctacaa cataaggttg tccagaaact tctcgtaaat
    12721 tttctttaat cttctcatcg tcgataccaa taattgagta tgcttgcagg aaacttgacg
    12781 tcgaagcgct ttgtccagct tctaccaatt tcttaacaac gtcttcactt aaaggtttat
    12841 tcttaaattt tctaacagaa tgatgctttt tcacaagatt atatacatgt tctgacacgt
    12901 tacccactcc tcgatacttt acaattaatt taaaatcttc atattgcaat tgtacacgtc
    12961 caaacttttt tatagcaatt atttgcttat aagttaataa ttgaactttt taaaatattg
    13021 tctcatactt taaaaatcca gtttctcact cattaccttc atccatctcg aacaaatatt
    13081 ctcacaccat tatcaataag gttgacttag ttgagaatac aaatcttctt tttttacatt
    13141 ttttacatta aaatcaagat attaaatata gacacaacct tttaaatcat caactacaaa
    13201 atccattatc aaagcacttt atattatctt tctttatttt aaaagtaatc attcacttta
    13261 caaagttatc cagttgacta ttctggatca aaatgtaata atgctaacta agagattaga
    13321 attattataa ttaagcgcaa tcaattgata gttctaattc atacttaaat tcttaggagg
    13381 aagatattta tgtcattaat taacaaagaa atcttaccat ttacagcgca agctttcgat
    13441 ccaaaaaaag atcaatttaa agaagttaca caagaagatt taaaaggttc ttggagcgta
    13501 gtatgcttct atcctgctga cttctcattc gtttgtccaa ctgaattaga agacttacaa
    13561 aaccaatatg aagaattaca aaaattaggc gtaaatgtat tctcagtatc aactgatact
    13621 cacttcgtac acaaagcatg gcatgaccat tcagatgcaa ttagcaaaat cacttacact
    13681 atgattggtg acccatcaca aacaatcact cgtaattttg atgtattaga tgaagctact
    13741 ggtttagctc aacgtggtac attcattatc gacccagacg gtgttgtaca agcatctgaa
    13801 attaacgctg acggaattgg ccgtgacgct agtacattag ctcacaaaat caaagcagct
    13861 caatatgttc gtaaaaaccc tggcgaagta tgcccagcta aatgggaaga aggcgctaaa
    13921 acattgcaac ctggtttaga tttagtaggt aaaatctaag gaggcattac aaccatgctt
    13981 aatgctgatt taaaacaaca acttaaacaa ctattagaac taatggaggg caacgttgaa
    14041 ttcgttgcca gccttggttc agatgataaa tccaaagaac ttaaagattt gttgacagaa
    14101 attactgata tgtcacctag actatctctt tctgaaaaat ctttaaaacg tacaccaagt
    14161 ttctcagtca atcgtcctgg cgaagaaaca ggtgtaacat ttgcaggtat tccattaggt
    14221 cacgagttta actcacttgt tttagcaatt ttacaggtta gtggtcgtgc acctaaagaa
    14281 aaacagtcaa tcattgacca aattaaaaaa ttagaaggtt cattccattt tgaaacattc
    14341 attagtttaa cgtgtcaaaa atgtcctgat gtcgttcaag cacttaactt aatgagtgtg
    14401 atcaacccta acatcacgca ttctatgatt gatggtgcag tgttccgtga agaatctgaa
    14461 aacatcatgg cagtccctgc tgtcttttta aatggcgaag aatttggcaa tggtcgtatg
    14521 acaatccaag atattctttc gaaactaggc agtacggcag atgcatctga gtttgaaaat
    14581 aaagaacctt atgatgtctt aatcgttggt ggtggtcctg ctagtggtag tgcagcgatt
    14641 tacacagcac gtaaaggttt acgtactggt atagttgctg atcgtatcgg tggccaagtt
    14701 aatgatactg ctggtattga gaacttcatt actgttaaag aaacaactgg ttctgaattt
    14761 tcttctaact tagcagcgca cattgatcaa tatgacattg atgcaatgac aggtatacgt
    14821 gctacagata tcgaaaagac tgacgaagca attaaagtta cgttagaaaa cggtgctgtc
    14881 ttagaaagta aaacagtcat tattgctact ggtgcaggtt ggcgtaagct aaacattcca
    14941 ggtgaagagc aattgattaa taaaggtgtt gcattctgcc ctcactgtga cggacctcta
    15001 tttgaaaata aagacgtagc agttatcggt ggcggtaact ctggggttga agcagcaatt
    15061 gaccttgctg gtatcgttaa tcatgttaca ttattcgaat tcgctagcga attaaaagca
    15121 gacaacgtgt tacaagatcg tttacgttct ttatcaaatg ttgatatcaa aacaaatgcc
    15181 aaaactactg aagttgtcgg agaagaccat gttacaggta tacgttacga agacatgaac
    15241 accggcgaag aacatctact taacttagat ggtatctttg ttcaaattgg tttacttcca
    15301 aacacatcat ggttaaacga tgctgttgaa ttaaacgaac gtggtgaaat tgtgattgat
    15361 cgtaacaata atacgaatgt tcctggaata tttgctgctg gcgatgtcac agatcagaag
    15421 aacaaacaaa ttatcatttc aatgggcgct ggtgcaaatg cagcattaaa tgcctttgac
    15481 tatattatca gaaactaaag tttcattcat ttcactgaca gtatcttaca tttgttaaga
    15541 tgctgttatt tttttatccc gacataaaat catgttatga taaacttaat atatacaacg
    15601 agatgtgcga taatgcttaa agtatgtacc tctcgcaaac aaatcaatat gaaaaatgat
    15661 attgagaaag aaaagtatgt gaacgaaatt attatgcttt tacgtacgat acctacgtct
    15721 aaattagtac aaccaattat gcagttattt atagataaag ctatccttaa caacgtccaa
    15781 aatgatgcat tattgataga aaaagcaact catgcacgta atcatttacg tctatggtca
    15841 ttgcaaggtc atcgtgacat tccaaactaa ttcaatgtta ctctattaaa catgaataca
    15901 tattttttca attaaagata aactaaggag ttatattaca ttatgaaaaa gagattacta
    15961 ctaagtacat ttttagcatc gacattaatt cttacaggtt gcgcgtccga tcaatctgat
    16021 aacgaagatc atcatacaag tactggcatc catgcaccta aaagcgctaa aaaattagaa
    16081 acaaaagata tctttaactc agataagaaa aatagtgata ttagtgatgc agagatgaag
    16141 caagctatag aaaagtattt gtctgtaaat agcgatatac ttgataacaa atatattatg
    16201 caacataaac tagataaaca aattgacagt caaacaaaag tgactgaaaa acaagcagaa
    16261 acacttagcc acttatctaa cttagctgtt aaaaacgatt tgcatttcaa gaaatttgtc
    16321 actgaaaaca atattcctaa agaatataag aaacccgttg aattaatgat gaattacttc
    16381 aaagctttaa atagtacaat cgccaatgtt gatgaagata ttgaaaagct tagctatcaa
    16441 ccgcaaaata aaatcaatgt tgtcgacgtg ccaacaaaat atgccggtga tgttaataaa
    16501 aagcaacaag ataaaattaa agatttctta aaatctaaag gtatcaagag tgatgttatc
    16561 gataaataac tgaaagccat cattgcgaaa tgtaacacat cattacatga gttttaattc
    16621 atgacaattc tttatcttta aaagtgacta atcaatttag ctaaagtcat catttgggca
    16681 tcaagcagca tattactgtt tgatgccttt ttaattgcct taaattctct atctatgcca
    16741 aaattcccat tagttgacgc atttgactat atacctaaca tattccatac ttatattgac
    16801 tctataaaca aaggatgatc atgatgaaag ttcgaattac ttttattatt ttagcaattt
    16861 tatcgacgat tgtttgcttg ttcttagcag caatgcatcc aacaggacca aacacagtaa
    16921 cttttgaaca gccatattta ttcaccctca acatcattat aatggtatta gtcgcattac
    16981 cttcacttgt tttagctata tatgattata tgagttttag aatttcttct gctattttac
    17041 aatttctagg ggctatctct tggttctttt tatcattgat attatcgctc acacaattta
    17101 caccttttac attagcgtca tttataactt caattatttt gttcacaagc acaattatca
    17161 cattagccat tggtggtaag tctgttgaaa agaatgattc cccttaaatt ccaaatgaaa
    17221 aaaaggttct gaaggccgct ataaaacaca gtttttcaga acctctatac ttctattcaa
    17281 tgatatatgg tttgcaattt tctaccttta aatccacagc ttctgccctt gaaactttgt
    17341 taaaataaac catcaaacaa cgaatgacaa cttgatgtgc aacaatgaca atatcatctt
    17401 tttgtgtatc ttcattgaca acatgattca taaaatgttc tacgcgttga tatacatctt
    17461 cataactttc tccttcaggc gctttttgtg aaaaactatg acgaaagtct ttaaagtttg
    17521 gatcattgaa atatttatca tatttcggat tcgcactgat ttcatcttta tattcaccct
    17581 caaatacgcc aagtgaacgt tctcttaata gaggggtagt cgttgatgca atgtcatatg
    17641 gaaaaatatg ttcaaacgtt tgctgtgttc ttaataagtc tgaaacatat acatgtttaa
    17701 tctgtttctc tttaaaataa tcacataaat cgtctgcact ttttgtgcca gtatccgtta
    17761 acggcacatc taattgtcca caaaaataag atcgaaaatg tttattatca taattcgatt
    17821 ttgattcgcc atgtctaact aaataaatcg tcataatatt actccttacc ttatgtattt
    17881 catatctacc ataacacttt gactactaat tcgatatcaa tcttaatatt ctattctaaa
    17941 aaaagaatta
//
LOCUS       DAKTIP010000034        17744 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAKTIP010000034 DAKTIP010000000
VERSION     DAKTIP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.034"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(18..1268)
                     /locus_tag="O0305_000907"
     CDS             complement(18..1268)
                     /locus_tag="O0305_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274060.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGVFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGVFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(1654..2679)
                     /locus_tag="O0305_000908"
     CDS             complement(1654..2679)
                     /locus_tag="O0305_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCY0274061.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(2852..3211)
                     /locus_tag="O0305_000909"
     CDS             complement(2852..3211)
                     /locus_tag="O0305_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCY0274062.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(3428..4459)
                     /locus_tag="O0305_000910"
     CDS             complement(3428..4459)
                     /locus_tag="O0305_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0274063.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQEDHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNVQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLNELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(4472..5233)
                     /locus_tag="O0305_000911"
     CDS             complement(4472..5233)
                     /locus_tag="O0305_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274064.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(5230..6204)
                     /locus_tag="O0305_000912"
     CDS             complement(5230..6204)
                     /locus_tag="O0305_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0274065.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKERSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(6218..6958)
                     /locus_tag="O0305_000913"
     CDS             complement(6218..6958)
                     /locus_tag="O0305_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274066.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGLQQKISDE
                     EINAQLKLVDLEDRGKHFPEQLSGGMKQRVALCRAHVHKPNVISMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(7300..7812)
                     /locus_tag="O0305_000914"
     CDS             complement(7300..7812)
                     /locus_tag="O0305_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCY0274067.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            7991..8194
                     /locus_tag="O0305_000915"
     CDS             7991..8194
                     /locus_tag="O0305_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274068.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(8256..9215)
                     /locus_tag="O0305_000916"
     CDS             complement(8256..9215)
                     /locus_tag="O0305_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0274069.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(9861..11348)
                     /locus_tag="O0305_000917"
     CDS             complement(9861..11348)
                     /locus_tag="O0305_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0274070.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGNPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            11775..12158
                     /locus_tag="O0305_000918"
     CDS             11775..12158
                     /locus_tag="O0305_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCY0274071.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            12165..12491
                     /gene="isdI"
                     /locus_tag="O0305_000919"
     CDS             12165..12491
                     /gene="isdI"
                     /locus_tag="O0305_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCY0274072.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(12556..13701)
                     /gene="cap8P"
                     /locus_tag="O0305_000920"
     CDS             complement(12556..13701)
                     /gene="cap8P"
                     /locus_tag="O0305_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCY0274073.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(13778..15040)
                     /gene="cap8O"
                     /locus_tag="O0305_000921"
     CDS             complement(13778..15040)
                     /gene="cap8O"
                     /locus_tag="O0305_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCY0274074.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPDASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILSFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTEACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKVLSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(15094..15981)
                     /gene="capN"
                     /locus_tag="O0305_000922"
     CDS             complement(15094..15981)
                     /gene="capN"
                     /locus_tag="O0305_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCY0274075.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQVDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEIIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(15981..16538)
                     /gene="cap8M"
                     /locus_tag="O0305_000923"
     CDS             complement(15981..16538)
                     /gene="cap8M"
                     /locus_tag="O0305_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCY0274076.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(16549..>17744)
                     /gene="cap8L"
                     /locus_tag="O0305_000924"
     CDS             complement(16549..>17744)
                     /gene="cap8L"
                     /locus_tag="O0305_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCY0274077.1"
                     /translation="KILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGWPYEY
                     SNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKYDQIL
                     VYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYINRHV
                     YKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKAYREQ
                     YDKILLYSGNMGQLQDMETLISFLKLNKDQSQTLTILCGHGKKFADVKTAIEDHRIEN
                     VKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMDKQSD
                     IVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREINTMKY
                     YNLLK"
BASE COUNT         5719 a         3380 c         2577 g         6068 t
ORIGIN      
        1 taaatattta aataaattca atttgtattc acttgttttt gtcccccaaa tacaccagca
       61 acaagcatgc tagcaccaat tgttaaaacg ataaacatat acagtcccat ttgtaatgac
      121 gttaagaaaa cacccaacac aatccctaac ctagctagtg tttctgaaaa atgaatacct
      181 aatgcattaa ctgcactata tgttcctctt ttagctttag gaataatttt aaagcgttgt
      241 tctgaaacta taggcgaata aataatttca cctacagtcg caattatcat aaaaacaact
      301 aataagccaa actgattaag ataggttaga ccactatagc caacaatata aatcagcaaa
      361 ccagtgatta aagctttttt aaaatctatt ttcaatacaa ctttcgagat tgaatacgtg
      421 agtaaaatga cgacgaccgt attaatcatt agcaagattg ctaacatctt agcacctgta
      481 atatcatatg aacctatact tattgtttca aactgatcct ttagtctaat agcaatatat
      541 gaggagattg aaaattcacc catcatgatg atactgaacc ccgaaatcaa taacatataa
      601 ttacggtctt tcaaaactaa tttataactg cgaaatatat tcattatttg taatttttga
      661 taacgacttg catgcctctt gtcatcactt tgctttactt gatttcggtc ttgaggtaac
      721 caaatatata aaataaagag tacaattaaa aatatacaag ctgctattaa gaaaagtagt
      781 aacatactgt agccatacat caagccacct aacaatgccc caatagctac cgataagttt
      841 gtcatccaat agctaatctt gtaaatataa tgttccacgt cttcggtaat tgcatccata
      901 attaatgtgt ccataactgg aaattgtaat ccccaaacga ttgtaaatat ggcatatgca
      961 acacaaaaac caataatttg ccacaattga tgtgacccaa atacgcccat gaacacaagc
     1021 attatcacca tcgtcgcttg ataaataagt actagcaact ttttcggaaa tatctcaata
     1081 aggtaaccag atataatgga caatggaaat tttagaacca ctaaaccaac aagatatata
     1141 ccgacaattg attgacttaa catatctgtt aaatatagtg ctataaacgg tataaatgct
     1201 gtcgtaataa ttagctgtaa aaaattgcta atcaatcgta ctttcaaagt cgtacttaag
     1261 cgtttcatta ataactacac ccctttccac atatatacac actaccattt atataaaatc
     1321 tattattaga ttatacgttg aaacaaagaa tttttagtta tacaaagtta tttaacaaac
     1381 aaatgcatcg actttacaat gcatcttgca tataatgcct caaaaaaatt agcactatat
     1441 gactatatat tattccaaat gaatgaaatt tatcaataat tgcgtaagtg aacacatatg
     1501 tgcgtaaatg ttaaatcatt gtcctaacat agctgtgact atacttagat gtaatcataa
     1561 aaaaactact gccatcttta caacagcagt agtgcttata tgattcattt atataaaatt
     1621 gaaagcgtta atcgctagcc cagcatcctt attctattta gctgtataac ttttactagc
     1681 aatacgacca cttgcaacaa taatatcttt atcttggaaa ggttcatgat tgaagaaacg
     1741 ctctaaaata tctttaactc catcttcaat acgtttttgt gcttctaaag tcatacctga
     1801 atagtgaacc gtcatagcat ttctaggcat tgttctccat ggatgatcag caggtgcagg
     1861 ttgtggatac caaacatcac cagcatatcc ttgtaaatgc tcggatgcta acgcttcaac
     1921 taacgcatcg cgatttacaa ttttaccacg tgcagtattc actaaataac tgtgtttttt
     1981 catacgactt aaaacatctt tatcaaataa gttatcagtt tctggtgtta atggtgcatg
     2041 aattgtaatc gcatcacttg ttgaaacaag ttcatcaaag cttacaaatt tagacaattt
     2101 atggtcttgt tgattgattg gatcatagtg ttgtaatgtt acattaaatg gcgctaatct
     2161 ttcagcaaca agttgtccaa ttcgaccaaa tccaaaaata ccaattgttt tgtgttgtaa
     2221 ttcatgcgca tgattaccta cttgagacaa gttccattca ccttctactg attgacgatg
     2281 accttcttca tagtttctaa gaagtattaa taaatccata accgcatgtt ctgccacact
     2341 aactgtatta cttcctgtaa cttcaacgac accaatattg tgttcacttg ctgccgctaa
     2401 atctacatgg tcagatccta cacctgctgt aattgctaat ttcaagttcg gtgctttttc
     2461 aatacgttca cgagtcatat atgcaggata aaatggcgca ctaataatca catccatatc
     2521 tggtaaatgt ttatctaagt cttcaccatt atctgctaat ataatgaact catgtcctct
     2581 ttcctctaaa aatgttttta atcctaatgc ttttttagta ttaagtaatt gattttcttg
     2641 accttctact gcttctggaa ataatgctac gattttcata tccatctctc ctaaatgata
     2701 aattttgact tccttaaagt acgtgttgca tgttgcttgt tttaaaaata tgacaaaaaa
     2761 acggcaccgt ttgacatacc acccccttga attattgtta aattcaaagc gaagttagca
     2821 aacagcaccg tgtaccttac accagcaaca cttattctat tgacttatcg aaaacaaata
     2881 ttgaaatgac ttcatcatca ttcaaatcaa tatctgtaaa taattttaca aacttagcgc
     2941 ctacaagact ttccatctca tttacatcta tttctttata catctgttta atcttctctg
     3001 tgcgtgtata atggagcatc gattcattgc gtttgtcgtt taggtaaaaa ctctcaactt
     3061 tactcaaaac acctgtcata tgtgcaatcg cccaattatc tttaaacgac actcgaatac
     3121 tattgggtcc tttacccacg tattctttac gataagatct tactagattc gagaattgtt
     3181 gttctttagt atgcgtatct atagtattca ccttctttaa aataagaagt tcattaattg
     3241 ttcacatttt cattatacac cttattaaca ctttttcaat gataacgatt attattatta
     3301 cgaaatgtat attttcctag ttaaaaggat ataacaaatt aacagaaaat gtttataaac
     3361 taagatttat attattttaa aaaatgtttc aacaaacgca tctaaacaaa aaatcagctt
     3421 atcacactta ccccttcaac tctgcttcaa gtttacctaa atgtcttaat gtcggtgtca
     3481 atgctgcgaa gaagaatcct tcttttaact tgcgaacttg tggcgaatat ggtgtgtacg
     3541 ctctagaacc accattgaca acagaagctt gatttacatc taacaataaa tagccgatgt
     3601 ccttcttcaa tgatattaat tcatttaaat gtgaagttaa gttaccgtca tctaatattg
     3661 cataatattc ctctctaagt tgacgataac gttttttaaa agcttcaaca tcatactcta
     3721 aatattgatt tattccgttt tgcacatttg aaaatgcatc aattaactct aaagaacttt
     3781 taattgagcc taatcctatt ggaatttggt aagcaataaa ttgcgggcga atagttgccg
     3841 caaactgctt cgcatcatgc gtgataattt gtgattgtgg cactacgact tgattcaatg
     3901 tgatttgata cgttgctgac ccgttgactc ctaaaaaatt ggttttttca acaagagtga
     3961 taccatcttg attggcacgt agaatgaaca tgacaaattc atctgatgat tcatgtttcg
     4021 aaatcgcacc aaaataatgg tcttcttgaa tattacttac agctggcata cgtccactga
     4081 caaccaattg tccatcaaca taagtgtgtt caaggttcaa cttttctaaa tcattaaatg
     4141 acttcatcgg attagacaat ccggtagcac ctaatatttc tccagataac aattgctgtt
     4201 gtaagtcatt atttaaatgt ggctgcgtgg cattttctaa atacgttgaa aaagctaatt
     4261 ggcaccataa acaaaatcct gttgtcaaac aagactgcga tacagcttcg attacttcag
     4321 cattttttct caatgccgcc tcaccgaaat aaccatctac aaataattgc tgaataaatg
     4381 ttctcggata atacgtccct tcatcaactt ctactaaatg aggatctaat tgttctttga
     4441 taagcgtttc taaagtcatc atcatctctc cttattcacc aaatcttcta agtataaact
     4501 gctcaatata actaatgaat cgatcaataa taaaaccaaa taatccgata aagaatattg
     4561 ctgctaaaac atcttctaag ttcaacatat ttcgtgcatc aacgattaaa aaacctaatc
     4621 ccgattgtgc accaatcatt tcaccagaaa ctaaaaatat ccaacttgtt cctaccgcca
     4681 tatgtatccc agccatgatt tgtttaaatg ccccgggaaa taatatattg cgatacaatg
     4741 accacccagt taaatttaaa tttgctgcta tttttaaata ttgaggttca atgtctctaa
     4801 cgcctttaat agtattgaac acaattggga aaaaagcagc gataaaaata atcgcaatcg
     4861 ctggcaaact accaatacca aaccatagaa caacaaatgg tgcccatgct atcggagata
     4921 tcggcctaat caattgaaat agcggttcga tagcgttgta tagccaacga ttccttccaa
     4981 gcaagaagcc caatggaata gcaaccaaca atgcgacaac aaagcccgct acaaatctcc
     5041 ataaactaat tgctaaatgt tggaaaattt ctccagtaac aatgaaagac catatacttt
     5101 ttcctacaag agcaggaccc ggtaacaata caggttggta atgcccaata ataatgacca
     5161 tttcccaaat gcctaagaaa ataataaatg tgataatagg taatataaat ttgttatttg
     5221 tgggacgtgt catgaacgcg atgcctcctt atacaatgac ggttcaacaa agtcatcata
     5281 tgcaggtgga ttaaacaaat gatgttgttt taccaatgtc gtaatttctt gatagccgga
     5341 tggcttaatt gttaaatcac cataggatgt ccatgccgct gactgtgtta aaacgtcacg
     5401 actttgttta aaatgatgcg tcataatgtc tacactttgc ttgcgatcat tcattttaaa
     5461 gccagacttt ttataatctt gtacaaatgc ttgcgctaca tccttgtgtt gatcaagcaa
     5521 ttcccctctc agtactagca cacagcaata cgcatcaggt ataacgtcat caccatgttt
     5581 caaagtctta cctttgccta acttttcacc cagtgcaccg aatggttcgg ctacagaata
     5641 ccctgtaatt ctgtgttcac tcaatgcggc tggcatttct gctggcgaca tttcatgata
     5701 gctaaaatgc cccggtttaa tctttaattg tttacgtaat tcctcaagta aaagataatg
     5761 tgttgaataa cgatgtggta taccaaaatg gtaatcatcg ccattattat taaattcatt
     5821 taagtgcata cctttttgtc ccataatgac attgccttca tgatggccca atgccacagc
     5881 ctttatattt gagcccttct gttttgattt catcgctagc tctattaaag ttgatgcacc
     5941 atcaatacga ccactgttta atgcgtccat taaatctggc caattattga atttaactaa
     6001 ttctagttta tatttcggat gattgtattg tgataataat tttttagtca tcatcaaatt
     6061 agctgaatgt gtaatcggca aatatccaat tttaatcact tgctgatttt gggcattttt
     6121 agaccgttct ttagacgtcc tttgccaatc acatcctgta attataaaga ttccaatgat
     6181 gacgattatg cttaaccttt tcatcgtcac tcactcctta taaataatat tcaggttcaa
     6241 cttgatgatg attcaatgca aatgtttcca taatttcatt acgaatctta agtaggtggc
     6301 tatcattacg actgcgtgga tgtgatgctg taatttcata ttgagaaata atattgcacc
     6361 cttcacctaa cagaacaatg cggtcggaaa gataaatagc ttcatcaatg tcatgcgtca
     6421 ctaaaataat agttgattgc gttttatgtt ttagttgcac tagttgatcc tgaagtttat
     6481 aacgtgtaaa tgcatctaat gcacctaatg gctcatccat cgatataacg ttaggcttat
     6541 gcacatgcgc tcgacatagt gccacacgtt gtttcatacc cccggacagt tgctcgggaa
     6601 aatgctttcc cctgtcttct aaatcaacta atttaagctg tgcgttaatc tcttcatcac
     6661 taattttctg ttgtaatcca atcctaatgt tgtcattaat cgttttccat ggcagcaaat
     6721 tatgatgttg aaatagcatt aaacaatctg gagatggctg ttgtttaatt tcgttatcaa
     6781 taatgacacg accagacgat ggatgaataa atccaccgat aatattgagt aaagtagact
     6841 ttccgcaacc acttttccct atgaaagtga ctatttctcc cttgctaatg tccaaattaa
     6901 agttatgaat tactttatgt gatccaaagt gatgttgtaa ttgttgaatt ttaatcatga
     6961 caagccccct tttaacccct actattttaa taggaattaa gtgtatatct aaagggctat
     7021 gccaaacaca gcccttatat cgtgaattgt tatatctata gatgattatg gtttggtatg
     7081 agtgctctta taacataagc gttattcaat taacgacgtt atattaattt cagctctact
     7141 aaatttcaat aaaatgattg ttttagacaa tacttgcatt tagttcatta gtttcttcat
     7201 atcaactact tacatttata gcattgagct attctaatta actgcaatcg aaatccatgc
     7261 caaacctata tacatatatc tcatcaactt gttgtcttat taaagttttt cgatgccatc
     7321 aatcggtgta tcaactgctt tgcgcgcgcg acgtaccgct tcttcatcag gtgcgttgta
     7381 taaacaaata catttagaca tatcttcaca tacatatgtg cgtttaaatt caacttctgg
     7441 cacttcttca taatgaacag aatttttctt tttacgtgct aaatattgat ccatcgtaat
     7501 gccttccgga atgttccaag ttacaaggta atcaacatgt gcatcaccat ttttaacttc
     7561 atctaaatct ttaccaacta agcgtacttc tttgactaat tgaatactaa tatctgcttc
     7621 tgttaaaaat tgtttagctt ctgcttcgtc atttgcttcc acaataaaat aaccatgagt
     7681 taaattttca gtagcttgta cttcaattaa tgttggaatt gtcttcgtag atagtgatgc
     7741 tgcctttgct tctagttctt ctttcgttga tgcaaaatca agattgttag cttctaataa
     7801 aaataatgtc attaataaga cctcctataa caatggttga aaaaatatta ctataattct
     7861 tatcggatta caaggatatt aagatatttt tttaaatatt tagataaaat tctgtaaaat
     7921 atattttcaa agagtataaa aatcatgtta gggtataagt aaataaacat atatataggg
     7981 ggctatcatt atggataaag aatctgtcgt agcaagttta gctagaaaca aaaagattgc
     8041 tgtagaaaca atggccggtc aaaggtacat catagaacgc attttacata caaatgatga
     8101 aaaacatatt catattttga aacctaaaga tgtcgtgtta gatgtagata gcatcaaaga
     8161 aattgacgaa aatcatttaa atgatgcaac ataagtataa aacgaaacgt gcgcctatga
     8221 catagattaa tgttttaggc gctacaatgt tttatttatg ttaatttgtc gaagtatggc
     8281 tcatctaatg gattcaattg caagttttca atttgcaacg ttgaatgtgc aatgccatat
     8341 ttttctttca acaatgatga tacttgatca atcgcttgat aatcatcacc ctcatatttt
     8401 ttatctaaca caacatgggc acttaatgaa taatgctctg ttgtaatact ccacaaatga
     8461 aattcatgta catctaatat gccatctatg tttttaatat ctgccataat ttgatcagta
     8521 tccaaatgtt gaggcacact ttccattaaa attaaccacg cattacgcgt aattttataa
     8581 ccaccacgta aaatgatgag tgaaattaca atactaatga ttgggtcgat gatgcgccat
     8641 cctgtaaagt aaatcaatac aactgcaacg atgacaccaa tagagttcaa taagtctccc
     8701 atgaaatgcc ataatgcact ttgaatattg atattgtctt cttgttttaa agaccttaca
     8761 aggataacag tcaaaataat attgacgagt aaaccaatac tagcaatcat aaacataatg
     8821 ccactttcaa ttggttgcgg ataaataata cgtacaatag cttcatataa aatccagatt
     8881 gaaattacaa ttaatgctaa accatttaaa aatgcagcta atatctcaaa tcttaaatat
     8941 ccaaatgtgt atcgtgcagt cggcttttta cttgcaaaat aaatggccaa catagataaa
     9001 ccaagtgcta atacatcact aagcatatga aatgaatctg acagtaatgc caatgaatta
     9061 gatactaaac ctccgacaaa ttcaatcact gtaaataaca atgtgatgat tagtgatgcc
     9121 cacagcgtct ttttagaact ttgttgaatt ttttgatgat tgacatgatg aaaatatttg
     9181 tatctgttta tcgtattcgc ctttttccca ttcatacgtt tgccactcct aaataaatgc
     9241 gtcgtattta atattgtcgc ttttaaaaat aattctttat agcttctcag gttaatatta
     9301 atgcttccgt aattaaccat gatttataca tcatcgtcat ttacatgaca actcattaat
     9361 ctttaccctt tatagcttta tgtaatatca ccttggaaaa cttttacagc acactcgcgc
     9421 tttgctttat tcctatcata tagaaaattt taataatgta cgagattatt gtgttagtta
     9481 cataaaaatg acaaaaaagt atctcaaatt tattgtttag tttcattaag ttcaaaaaat
     9541 cgcaataact ttaatgattg ttccgcatat taaaacaaac gatgactttg catattgaac
     9601 ttcatatttc taaatcacca taattaataa acaactctgt ccttataata gccgcatcgc
     9661 aaattagcat aattaaaaac agcagtaaga tgatttgcaa ttaaaaacca tcctactgct
     9721 attattttag tcattatatc ctaatatcat gatacgtgtt cctatatatt ttgttttgct
     9781 taaaatctaa acaacgccgt taaatgttaa gacttaccgc tagactgacc tacaaacacg
     9841 cttcagaaac gatatttatt ctagtacaaa ccttttaaag cattgcttgt atcaatataa
     9901 atatttttaa cttgttgata gttacttaac gcacctttat aagtttctcg accgatacct
     9961 gattttttat aaccaccaaa tggtgcgcct tctggtactt ggttgtaagt gttaatccaa
    10021 atacgtcctg tacgtacagc tttagcaata tttaatgcac gtgtgatatt ttgagaaaat
    10081 acaccgcctg ctaaaccata ctcagaatca ttagctatat caattgcttc ttgatcgtcc
    10141 ttcactttaa tcactgttaa cactggtcca aatatttctt cttgtgctaa tttgtgatga
    10201 ttgtctggca cagcaattaa tgtcggctca aagaagaacc ctttatctaa tccattttca
    10261 gttaagcgat gaccgcctgc taaaatttgt gcatctgatt cttttgctgc atcaatatat
    10321 gattgaattt tatctaattg atccttacca gtttgactac ccatttgtgt agcttcatct
    10381 tgtggatttc caactttaat atttgaaaat gcctcttgta aacgtggcac caattgatca
    10441 taaatttttt catgaactaa taatcgagaa cctgcactac atacttcacc ttggttgaat
    10501 aaaataccta actgaatacc ttcaactgca aggtctaaat tagcatcatc taatatgata
    10561 ttggcgcttt taccaccaag ctctaatgta gcgggtacta gatgttttgc tgcagcttcg
    10621 gcaacttgat aacctacatc agttgagccc gtaaatgata atttatctac accatcatga
    10681 ttgaaaattg cattacctga ttctgaacct ttacccgtta gtatattgac aacaccttta
    10741 ggtaatacct cttggaaaat tttagcaact tccaataaac ttaatggtgt tgaagacgaa
    10801 ggttgaatca caattgtatt acctgcagca atggctggcg caatcttcca tgcagctaat
    10861 agcattggga agttccaagc aacaacagca cctacgacgc caatcggctc atgtcgtacg
    10921 atactcattg tgtctttatc gatatcattc actgtacctt cttctgtttc aataacactt
    10981 gcgaaataat ggaaatgtct tgcagcaaat ggaatatcaa ttgctgttgt ctcacgaatc
    11041 ggtttaccat tatttaatgt ttcaatcatt gcaattttat ctttttgtgc cattaattta
    11101 tcaccaatat cacgcaacat ttgtgcacgt tctgatttag aagttaatga ccatgattca
    11161 aatgcctctt gcgccacttt gactgcatga tcgacatctt tatcttttgc tcttgtaata
    11221 tgtgatagtg tttctccagt tgctggatta gtcacttcga ttgtttcgtc actgctacct
    11281 ttaacaaatt ccccattgat aaataaacca taattctctg caatataatc tcgaacgttt
    11341 actgccatat tatctctcct ttgtttattg gctataatat aaatctctta gcatcatatt
    11401 taataatgct tttaaacgat atgattcaca ttatatagta agcgtttaca ttttataaaa
    11461 taatcagctc acagaagtct catttttaaa acttttgcgt cgtttttgca cataaaaata
    11521 atcttatttt cgaaaacata ttgcactttt caaaaataaa acacataata taactataat
    11581 acttgagaat aattctcatt aaatagtttg gttgttatga aagtcgcgct taaaatgcgt
    11641 taatgtgaca aggataattc aacatcgttt cattttactg agtcattgca cttatcatac
    11701 acattatatt tagcatgagt tatattacta aaacaatatt acatgcgcat tagtactaga
    11761 atggagtttt ttcaatgcga cttattttaa tcgtcattgg cttaatattt acagcgcttg
    11821 gtattgcagg tgccgtatta cctttactgc caacgacccc ttttttactc gtagcagttt
    11881 tttgttttgc tcgaagttca gatcgctttt acaattggct tattaatcaa aaaatttata
    11941 aagaatatgt agaaaacttt tatttacatc gaggctacac gctacaacag aaaattaaaa
    12001 ttttaattag cttatacatt gtaataggtt tttcaattta tatggtggat gttcttgcag
    12061 tccgtgtagg attaatcata atggttatca tacaaaccgc tgtactcttt acatttgtaa
    12121 aaacattacc caaatcaaat cataaaatag aggagtgatt gcccatgttt atggcagaaa
    12181 atagattaca attacaaaaa ggcagtgcgg aagaaacgat tgaacgtttt tacaatagac
    12241 aaggtattga aactattgaa ggcttccaac aaatgtttgt cactaaaaca ttaaataccg
    12301 aggatacaga cgaagttaaa atcttaacta tttgggaatc tgaagatagc tttaataatt
    12361 ggttgaattc cgatgtattt aaagaagctc ataaaaatgt acgtttaaaa agtgatgacg
    12421 atggacagca aagtccaata ttatcaaata aagtattcaa atatgatatt ggctaccact
    12481 atcaaaaata aatatattaa aaacccctag tcaccgctgg ttgtctaaac caacttcatg
    12541 attaggggtt ttttattatt tgtgacgtaa aggtacgaat tcatccggct tgtctgtgcg
    12601 caatccaaaa taatattcta ttgcttcaca aatacgtcgt gatgcttgtc catcaccata
    12661 tggattattc gcttgactca tacgttgata cgcttcatca tcctcaatca attgtttcac
    12721 atttcgaaca atattgtcat aatctgtgcc aattactctc gatgttcccg cctcaacgcc
    12781 ttcgggacgc tctgtatgat tccttaatac caacacaggt tttccaaatg taggagcctc
    12841 ctcttgaata ccaccagaat ctgtcagcac gaggtacgat tgatttgtaa aattatggaa
    12901 ctcaatcgca tctaatggct caattaattc aatccgattt ctcccagata aatatttttc
    12961 ggcaatcgct cttacctttg gattacgatg cattggataa atgaagacaa catctttata
    13021 ttcatctgcc aaatctctta ctgctttaaa aatctgatgc atcggttccc caatattttc
    13081 acgacgatgc gctgttagta aaacaacttt cttgccttta tgtttattaa taatcgttga
    13141 aacaaaatca ttttgaactg ttgttgacaa tgcgtcaata actgtatttc cagtaataaa
    13201 gatacgctct ttgtctttgt tttcaaaaag taaattttta gctgcaatta ctgttggcgc
    13261 aaaattcaat tcagcaatat tacttaccat gactcgattt aactcttcag gaaatggtga
    13321 gtatttctga tgtgttcgaa gtccagcttc tacatgtccg accggaattt gatgataaaa
    13381 tgctgccaaa cttcctacaa aagtcgttgt agtatcacca tgtactaaaa tcatatccgg
    13441 ttgttcctca ttaatgatgc tatcaagttt agcaagtgca ttcgccgtaa ggcctgctaa
    13501 tgtttgttga tcttgcataa tatttaaatc atgatcagct tgaatatcaa atatacttaa
    13561 cacactatct aacatatctc tatgttgtgc tgtaatcaca atgttcgctt caaagttccc
    13621 attatgatca atttctttta ctaatggtgc catttttatt gcttcgggtc tcgtaccgaa
    13681 aataaccata atttttttca taacattatc ctctctgaag ttcaaacaca tgacagctta
    13741 tctttcctta atcatgtatg ccctagtttg acacatttta tttgtcgata aaattaaata
    13801 tattgccata attataatac gatacatctt caaatgatga tttcacaaca ttttttgtat
    13861 caaaaatcac tttatgcttc attttatcaa aatgactgtc cgataaattt ttaaattctg
    13921 agtggtcact taaaatcaat actagcgatg cgtctttgac agcatgtgac atatcatgtt
    13981 ccacaaaatc taattcaaca tgtggatcat aagcacatac ttctatgtct ggttcttgat
    14041 ttaatagctc ataaatatca aatgctggtg attctcttat atcatcaaca tcacctttat
    14101 aagttaaacc aaatactgtg actttattcc cgctcaacac tttgatgatt tgcttcgttg
    14161 tatcaacaac ataggccggc attgaattat taatttcacg tccagtttga attaactttg
    14221 cattttcagg gtctttagca ataataaagt acggatcaac agctaaacaa tgaccgccta
    14281 cacctggacc aggctgatgg atgttaacac gcggatgttt gtttgccatt tcaatcacat
    14341 ctaatacatt aatatttaag ttattgcaaa tttttgttaa ttcattagct aaagcaatgt
    14401 tcacgtctct atatgtgttt tccattagct tactcatttc agcagtacgt gcatctgttt
    14461 caatcatttc tccctgaacg aatgtgcgat agacacgttt acccgcttca atacaagctt
    14521 cagtcacacc gccaatgata cgattgttat gaactaattc ttctaaaatt tttcctggca
    14581 gtacacgttc tggacaatgc actaaataaa tatcttcacc tattgtaaac cctaaatttt
    14641 caatgactgg ttttacaaaa tcatccatcg ttttaggcgc aattgtcgac tctacaataa
    14701 tggtatttcc tttttctaaa aatgataaaa tactatctaa tgcacgcata actagcgaaa
    14761 tgtcacatga ccggtactga tcatcattat tcggcgtcgg aacggcaatg ataaaaacat
    14821 cagatgcatc tggcgttgta gataccttca attttcccga tgacagtacc tcttcataaa
    14881 cctcttgtaa tccaggttct tcaatactaa tttgaccact ttgtaactta tcaatcgttt
    14941 gctgattaat atcaacacca agcacatcga cgccatgttt tgcaaacata attgatgttg
    15001 gtaaaccaat ataacctaag ccaactactg ttaacttcat actataacct ccaaataaaa
    15061 cgatttgtat gttgatttta tttaatagat gacttatgcc ttatctttgg ttgtcgtttc
    15121 atccatgatg tccgcaataa caagtgacat ttttccagga ataatttcaa gtgcattatt
    15181 attttcatat aacgtattgc tgtatattaa attgccgaat aattttctaa agaccgacaa
    15241 cttattaaaa tacttattta gcattgaagg catgttgatc aataccgttt tacgatgtga
    15301 ttggcgacgt atttcataca ttactgacga tgtatcaaag taaaaactat cttgaggatg
    15361 gtacacacct gtcacttcta atgatattaa ttgatcaata aatgctgtca gatgtttaat
    15421 atataatgca ctgcgctgat tgttaatatt gggaatgatt ggcaatcgct ttgacaattg
    15481 cattaaccgt tggaaatttc ctgggcaatg tgcaccataa atcattggtg gtctcacaat
    15541 tgctacttta aacgaatcac taatcaattc ttgtaatgct tgttcagcga actttttgga
    15601 aataccatag ttggtcgtag ggttcattgg tgtttgtgta tcaacttgat ctgatttacc
    15661 aacatgacct tcttttccat aaactgccat agtactcata aaaataaatt gtttaacgtc
    15721 ttcagcttta gccttttgtg ccaattgttt cgtcagcaac atattcactt gcatataatc
    15781 agatagcctt gcttgaggtg aattgttgtg aaccaaagct gctgtatgaa ttaaaacatc
    15841 ataatctttg aacgaggtcg acttccataa ttgattccta acattaattt gatctacttg
    15901 atgtccttgt tcaataagct tatcttttaa agcattaccg atatatccat gtacgcctgt
    15961 aattaaaata ttttttctca ttagtgatgc acaccttctg aagtaacgat atttttaatt
    16021 gttttatata tgatatacat atcaagcatc atagattgat gtgttaagta ataatgatca
    16081 tacgctactt tttgatcatc agtgatatca tctctcccca tcacttgagc tagtcctgtc
    16141 acacctggtc taatcgtatg cacgttcgct tttgtacgtt tttcgattaa ttcgtattga
    16201 ttataaagcg ctggtctagg acctacaatt gacatttctc cttttaaaac attcaataat
    16261 tgtggcaatt catcaataga ggtcttacga atgaccttcc ctgtctttgt tatatacgat
    16321 gttgaatcca ttaaatcagt tgcaacatta ggtgtgtcta ttttcattga tctaaactta
    16381 taaatattaa acaattcatt attaatcgtc ggtctttttt gtttgaaaat ggctggtcca
    16441 ggtgattcca ttttaattag taatgctgta attaacagaa tcggacttaa aactactaaa
    16501 ccatatattg aactcactac atcgaataat cgcttcataa ttatctcctc acttcaacag
    16561 attgtaatac ttcatagtat taatttctct cgtatattta tctttaaaca gttgatgtgc
    16621 gcgctcaccc atctcgtgca attccttact cgagtgagtg ttgatgaagt tataaatggc
    16681 atgtgcatcg ccattatcaa tttggatacc cgcatcatat tgttcaacat gttgaacgat
    16741 atcagattgc ttatccatga tgagtaccaa cgctttctta gctgcaagat agccataatt
    16801 cttgctcggc acgcctaaac cgacgccttc tttaatcagc gatgcaatac atacatccgc
    16861 aatttttaat acgtcagcat agtctgtacc tgttaaaaac tcaaacattt taacattttc
    16921 aatacgatgg tcttctattg ccgttttgac atctgcaaat ttcttaccat gaccacaaag
    16981 tattgttaac gtttgagact gatccttatt taattttaaa aatgagataa gtgtctccat
    17041 atcctgtaac tgccccatat taccgctata caataaaatt ttgtcgtatt gctcacggta
    17101 agctttaaat gtgtcattat agatacgatt gtcttgtaat tgacgcatgt cataccagtt
    17161 aggaatcaca tggatattgt cagcattttt agaaatttga tgatttagta agtagttttt
    17221 catttccgta ccaaggacaa tgacattttc agcattcttg tagacatgtc tattaatgta
    17281 acgcatcagc ttatcaatca tgctacctgg acgagttgca cctgtcttaa tcgcattatc
    17341 aggtgctata tcatacacca caaaagaata ttttttctta agcagtctgt gtaaaacgtc
    17401 tggtattaat ggcaagattg gtggattaga gtaaacaaga atctgatcat atttcaacat
    17461 tttaggtata ttaatcacga attttgaaaa taaactaaag aaattgatga tccttccaac
    17521 cttacttttg ttattaaacc tcgaatactt gagacgtcga atgcgaatac cacgatgcat
    17581 ctcggtttta gaaacctgtt tatgattact atattcatat ggccatccac acatgacatc
    17641 gacattaatg tgattcgcaa ttaaatcttc cgccaattga gttggtaacg tcgcagaaga
    17701 tacatattcc ggataaaaat actgacataa aatcaaaatc tttt
//
LOCUS       DAKTIP010000035        17453 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAKTIP010000035 DAKTIP010000000
VERSION     DAKTIP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.035"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(203..562)
                     /locus_tag="O0305_000925"
     CDS             complement(203..562)
                     /locus_tag="O0305_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCY0274078.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(575..811)
                     /locus_tag="O0305_000926"
     CDS             complement(575..811)
                     /locus_tag="O0305_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCY0274079.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            1128..2192
                     /locus_tag="O0305_000927"
     CDS             1128..2192
                     /locus_tag="O0305_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0274080.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(2251..2574)
                     /locus_tag="O0305_000928"
     CDS             complement(2251..2574)
                     /locus_tag="O0305_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCY0274081.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            2675..2917
                     /locus_tag="O0305_000929"
     CDS             2675..2917
                     /locus_tag="O0305_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCY0274082.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(3180..4355)
                     /gene="dltD"
                     /locus_tag="O0305_000930"
     CDS             complement(3180..4355)
                     /gene="dltD"
                     /locus_tag="O0305_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCY0274083.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLIKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKEYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(4352..4588)
                     /gene="dltC"
                     /locus_tag="O0305_000931"
     CDS             complement(4352..4588)
                     /gene="dltC"
                     /locus_tag="O0305_000931"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCY0274084.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(4606..5820)
                     /gene="dltB"
                     /locus_tag="O0305_000932"
     CDS             complement(4606..5820)
                     /gene="dltB"
                     /locus_tag="O0305_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCY0274085.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(5817..7274)
                     /gene="dltA"
                     /locus_tag="O0305_000933"
     CDS             complement(5817..7274)
                     /gene="dltA"
                     /locus_tag="O0305_000933"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCY0274086.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVNR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(7290..7442)
                     /locus_tag="O0305_000934"
     CDS             complement(7290..7442)
                     /locus_tag="O0305_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCY0274087.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(7490..7600)
                     /locus_tag="O0305_000935"
     CDS             complement(7490..7600)
                     /locus_tag="O0305_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274088.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            complement(7854..8813)
                     /locus_tag="O0305_000936"
     CDS             complement(7854..8813)
                     /locus_tag="O0305_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCY0274089.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            complement(8846..9625)
                     /locus_tag="O0305_000937"
     CDS             complement(8846..9625)
                     /locus_tag="O0305_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCY0274090.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            complement(9625..10059)
                     /locus_tag="O0305_000938"
     CDS             complement(9625..10059)
                     /locus_tag="O0305_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCY0274091.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            10157..10423
                     /locus_tag="O0305_000939"
     CDS             10157..10423
                     /locus_tag="O0305_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCY0274092.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            complement(10502..10885)
                     /locus_tag="O0305_000940"
     CDS             complement(10502..10885)
                     /locus_tag="O0305_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCY0274093.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(11015..11932)
                     /gene="lipA"
                     /locus_tag="O0305_000941"
     CDS             complement(11015..11932)
                     /gene="lipA"
                     /locus_tag="O0305_000941"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCY0274094.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            complement(12016..13335)
                     /locus_tag="O0305_000942"
     CDS             complement(12016..13335)
                     /locus_tag="O0305_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0274095.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            complement(13362..14189)
                     /locus_tag="O0305_000943"
     CDS             complement(13362..14189)
                     /locus_tag="O0305_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0274096.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            complement(14202..15050)
                     /locus_tag="O0305_000944"
     CDS             complement(14202..15050)
                     /locus_tag="O0305_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCY0274097.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            complement(15098..15196)
                     /locus_tag="O0305_000945"
     CDS             complement(15098..15196)
                     /locus_tag="O0305_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274098.1"
                     /translation="MRFYFTNLIKRKHQAEDRRKILIKQKHQTKVR"
     gene            complement(15263..16330)
                     /locus_tag="O0305_000946"
     CDS             complement(15263..16330)
                     /locus_tag="O0305_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCY0274099.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSEQIISRLKAANVKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVITKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELIHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            complement(16344..17384)
                     /locus_tag="O0305_000947"
     CDS             complement(16344..17384)
                     /locus_tag="O0305_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0274100.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
BASE COUNT         5494 a         3175 c         2562 g         6222 t
ORIGIN      
        1 tagcctgatt tcgattaaaa atgtatccgc tatcacgatc atttaaaata ctatttatag
       61 tcagattctt aattttcaat aagcttttta ataaaacaaa atatctttac gcaaaaatag
      121 gacataatca aatatgtcct taatcgttat tgatatcaat ttgtatccca aaaattgggt
      181 taaccattaa aaagttattt tattagcaat tttcaggatt acctgcaact tttgcagttc
      241 taaatgaact accacagcca catgaagcaa ttgcattagg attgtcgatt tggaaaccgc
      301 cacccattaa tgattgctta aaatcaatag tcgtaccatt taatacgggt gcatcttttt
      361 tgtctactaa tacttttaat ccaaagtatt ctaagacttc atcattttca ccaggcgctt
      421 cttctgcacc cataccgtat gttaaaccag tgcacccgcc accattcact ttaattttta
      481 aatagccatc tggcatttca tttgctttaa gcatatcttt tacttcgtaa gcagctgctt
      541 ctgttaatat aactgttggc atgataactc ctccttaaaa aatccaagtt tcttttatat
      601 gtgcatatat attttgtaat aattcttccg gcgaatcacc ttcaacaata tcaccattta
      661 ctaaagcata caacccggct gaacatatac cacaatgtgt caggcaacca tactctaaca
      721 catcgacatc tgggtcattt tccagttgat taaaaacata atctccacct tttgccatgt
      781 tagagagaca aaattctacg atcggattca tacttcacct tcttatttca tttgttacaa
      841 tattatagca ttttaaaact ggtattttaa catgatgtgc tcaattagca acaactgatg
      901 tttcttatcc cagttatgta atagtgcctt agttagtaca aatatttatt tgtagtcatt
      961 ttcaaaaaaa tatataataa gcatataaat acatatttac ttttttaggg gttattgatg
     1021 aatcaaagac acttgcattt tataactgtt cattaacaaa tatatctatg cactttgatc
     1081 ttttcaaaat taaagtaaat attaacatat aaaaggggta cttcattatg aaaaacttag
     1141 ttttgttagg cggcggatat ggtaatatgc gtatcatgtc acgcatttta actacttctt
     1201 taccacaaga ttatacagtc acattagttg atcgtatgcc atttcatgga ttgaaaccag
     1261 aattttatgc tttagctgcg ggcacgaaat cagataaaga tgttcgtatg aaattcccta
     1321 atcatccaca agtgaataca gtttatggtg aaattaacga catagattta gatgctcaaa
     1381 ttgtctcagt cggtaattct aaaattgatt atgatgagct aatcattggt ttaggatgtg
     1441 aagataaata tcataacgtt ccaggagccg aagaatatac acatagtatt caaacactct
     1501 caaaggctcg ggatactttc catagtatta gtgaactacc agaaggtgct aaagtcggta
     1561 tcgttggtgc tggattaagc ggcatagaac ttgccagcga attaagagaa agtagatcag
     1621 acttggaaat atatctttat gaccgtgggc cgcgaatttt aagaaatttt ccagaaaaat
     1681 taagtaagta tgttgcgaaa tggttcgcca aaaataatgt taccgttgtt ccaaattcaa
     1741 atattaataa agttgaacct ggtaaaatat ataactgtga tgaacctaaa gatattgatt
     1801 tagttgtatg gacagcagga attcaacctg ttgaagttgt tcgtaacttg ccgattgata
     1861 taaatagtaa tggacgcgtg atagttaacc agtatcatca agtaccaaca tatcgtaacg
     1921 tctatgtagt tggtgattgt gctgatttac cacatgcgcc aagtgctcag ttagccgaag
     1981 ttcaaggtga tcaaattgcc gatgtgctta aaaagcaatg gctaaatgaa ccattacctg
     2041 acaaaatgcc ggaactaaag gtacaaggta tcgttgggtc attaggagat aagcaaggtt
     2101 ttgcatatat catggaccgc actgtaacag gacgactcgc ttctatttta aaatccggcg
     2161 tactttggtt atataaatat cacaatggtt aaaattaaaa agcaatgtca gttcacacta
     2221 ttactgacat tgcttttagt tatgtgtata ttattcattc acaagctttt ggtctataaa
     2281 tcgagtgatt tgctttgttt gtatataacc atctgctaca tattcatcgt tcattgtaat
     2341 taatggataa aatagttcat cttgttctat tctttcaata aattgtaaat catgatctgt
     2401 taagttgtca ttatcttttg taatatctat atacgtatat ttaaatgata tatttgggta
     2461 ttttcttttt aatagcggct gtagccagtc ataaatatct ttcgatgttg gcgcattaac
     2521 gcaacttgca catataacat ctgccccata aactaccaca ctcacgtgct ccatatattt
     2581 atcccccatt ggtttgatag atttttatta cactatctat tataatatac taataaagat
     2641 tatcaaattc atctctcgaa aggagacttg cctgatgcct actgaagata caacgatgtt
     2701 tgatcaagta gcagaagtta ttgaacgtct tcgtccattt ttattacgtg atggtggcga
     2761 ctgctcattg attgacgtgg aagacggtat tgttaaatta caattacatg gtgcatgtgg
     2821 tacatgccca agttctacaa tcactcttaa agctggtatt gagcgtgcat tacacgaaga
     2881 agtgcctggt gtaatagaag tagaacaagt attctaatat aaagatatta agttaacaga
     2941 acatattatg aataacaaca aagacaggaa tactgaatta tttataatca gttattacct
     3001 gtcttttttc gctgtcaaat tggactgatg ggaaagttat tttatactat ttatctgatt
     3061 aattatttta ttattgccac atatccaagc acatattact attctattct atgaaattta
     3121 aaatataaaa atagcacata cgctcaatca aaatcgttga gttatgtgct atttgtattt
     3181 taatttttag gtttatctac ttcaggttgt ggttcacctt tcatatgttt cgcaatttgc
     3241 tcatccatat aaacccaacc tttccaaccg atgtgtacgg catcactgat aacatatttt
     3301 tcatattctt tatcagtcat atcgtaaatt ttaccaccat tatctacaac agtagaatgg
     3361 atttttttat aaactgcttg acgacgttct ttatcgatac caatgtggtc ataccataca
     3421 ccgtttgatg gaatacttac atattgaaca tctgcaccag cagcacgcat tgtttttata
     3481 agtaattcta aatcttggaa ttctggagaa ttaacattga attcgtagtc acgtctaact
     3541 ttacgcttac tttcttgaat taatttccag tattgatctc taataccaaa tttattcgat
     3601 gtagtatcag ctttaccaat ttcaactgct ttttgtttca tctcatccca agaagcgtct
     3661 ggttttgtag caggtttaac atgttctaat ggagatttat ccattgcaaa caatgattta
     3721 atcgcttcta ttttaatcaa ttgattctct ttaaaaccag aaatataact atctttagtt
     3781 tctttagggt ttttagcata agatttcaag tattctttat tgtgtacatg tggaaactgt
     3841 aataaacgtt gtgcataacg acgttttaat tcagtagaca tgtttttctg ctggaacatt
     3901 tgattaattt gagtttgaga catacgagca tcaaagtttt gattcgttaa accatggttt
     3961 gtaaaccatt gtggtgaaat aataaatgtt aatttcttac cttttaattt atcatactgt
     4021 gatgcaagtt caactgcgtt aattaagtct gtagaaccac cagcacctaa taagaatgct
     4081 tttttgttgg cgttatgctt atttaatgca attgcaggat taaatgggtc atctttacct
     4141 aattcactag agccgtatat aggataatac ttgtttgatt cgtataactt atcttgaatg
     4201 agtgtgcctt ttagtacttg atctgtcaat gaagttctat tatcttctac agtcttttca
     4261 tttactaatc ctgtaaacca actagcaggt aataatagaa agacaatgaa taccgctcca
     4321 ctaattaaaa tgggtaaaaa aggttttaat ttcatcgtaa ctcttctaat gcttcaacga
     4381 ttttatttgg tgttgcccac tcatctctat caaagtccat aatagatact tcgatatcaa
     4441 gtttattttg aatctctaat aataatccaa ctgtttggaa agaatcaata ataccttctt
     4501 caaaaatttc tacgtctgga ttttctttta caatatcatt ttctgctact tctgctaata
     4561 aatttaatac ttgttctcta aattccataa ttaaattctc ctttattata taagtttacc
     4621 tgagaagatt aaaaagccaa atgttacaaa gtggaatgta atcacaatgc taagtgctgt
     4681 tgtgaaacca ttttgccaac gtggcggatg tttcttacgc caacgttcat aatagccata
     4741 acctataaac aatgctgcat ggtataaacc ataaacaatg taatacactt cgataccatg
     4801 ccaaattccc attatgaaga agttgattaa gaatgccacg ttagacattg caaattgact
     4861 cttcaataat tttttacgag acatgtagaa taaagatctc atgtaaatac aatctctgaa
     4921 ccagaatgat aatgtcatat gccatctatt ccagaaatct ttaatatttt tcgctttgaa
     4981 aggtttatcg aagtttggtg gtgttttaat accgaataaa taactaaatg ctatcgcaaa
     5041 taaactataa cctgcaaagt caaagaataa atataagctg tatgcgtaca tatataacca
     5101 caaattgaca aagccatgta agtctaattg taacggcatg attgcatatg tgttaataaa
     5161 gtaagcaaca atatatttat acaagaaacc aagcataatc atgtgaattg cttttaatac
     5221 taattcacga tattcattgc ctgttggtac ttttttatcg tctttaacga aacgtttgta
     5281 acgatcgatt ggtccagatg aaatcgttgg gaagaatgaa ataaattgaa ttaatttcca
     5341 tactttaatt tctttgatag aaccatcacg aatttccata attaactgca cacttttgaa
     5401 tgtaacataa gaaattccta agaaaccaac aaattcaatt aatttacttt catggaagtg
     5461 aatctgatgt ccacctaacc atgtactttg taacactttc acaagtgcta atggcaatat
     5521 tgataaaacc attacagtta caaattttga aaatgaatta ttttttggtt ttgaatgata
     5581 ataaaacatt atcaataaaa cttgccatac gacgtaaata ataaaactaa ttaattgaac
     5641 acttaaatac ttttggtcaa acagattatg tttatcagaa gaaaagatta acacaatcat
     5701 aaatgctgta actacgccat tataaatgta acttcgctta cctaaaaatc caagtataat
     5761 gactggtaat aatgcaatta aagcaattaa gaagaatgta aaatcaccat atggaatcat
     5821 ccgttaatta cctctgcaat tttctttctg tcaattttac catttgaagt caatggcaat
     5881 tgttccatcc attcaaactt tctaggaatc atatactctg gtaagcgtga tttcaagtca
     5941 tttttaatat ttttagtcat ttctgcattg tccgtaactt cagtcgttgg cacaattgca
     6001 ccaattaaat gaataacttt atcattttta tatacaggta caacaatcgc ttcttttacg
     6061 aactcagact ggcgtaattg tgtttcaatt tcttctaatt ccattctgta gccattcaat
     6121 ttgatttgga aatcaatacg accttgaatg aaccattgac cattttcaaa cttcgcttta
     6181 tcaccagtgt gatatgtacg aataccgtca tcgaaattaa atacttcagc tgttttttgg
     6241 tcatttttta agtatcctaa acttacactt tgaccttcga taacaagttc accttcatct
     6301 gtagtagata atcttgcgcc tggtctttca acgccaacag gtaatgtcgg atattgatct
     6361 aagatttctt gtgtaatttg aatacttgta actgctaccg tagcttcagt tggaccatat
     6421 gtgttgtaaa tcgtcgcact tgggaaacgg tttactaacg cttttgctgc tctgtgaggt
     6481 agaatttcac cacagaagaa gaattcgtta agactaccat attgttcttc attaagcgtt
     6541 ggtaataata aacacatttc cataaatgat ggtgttgata cccaaatgtt aatcggtgtt
     6601 gctgttagca tttcatttaa taatttaggt ttattaatca tgtttttatc tacaagattt
     6661 aatgtaccgc ctgatgctaa acatggataa atagccatta cagataaatc aaatgaaaat
     6721 ggcgcttggt taagccattg ttgttcattt cctgatttat taagttctaa catccactca
     6781 gtaaattgaa ctaaacttgc atattcaatt tgaacacctt taggttcccc agtagaacca
     6841 gatgtaaaga ttgtgtatac tgtgtcgtta tctttaatct gactatcaaa aattactggg
     6901 tcttgagatg ttttaatatc ttctattgta aatacttcgc cttctaaact ttcaaatgat
     6961 tcatcagtcg tattaaatac aaactctggt tgaaccttgt taataatcat tttaatacgg
     7021 tcttcaggaa ttgaagtgtc tacaggtaca tatccacatc ctgctttaat ggcaccaatc
     7081 atcccaacaa tcatatatgg tgacatgtga ccgaataaaa tcatcggttt cttactacct
     7141 tgtaatcgat gtgctaattt actagactca tccattaact gttgataagt taattcatca
     7201 gttgtgtgtc taacagcaat gctttgtgga tttgcatccg caaacgcttg cagcttgtta
     7261 ataatatctg tcatattaag tctccctcat tagaactcat tataaatgaa gttattgtgt
     7321 gtgtcgccac tgccataaat taaatataaa gtaataaata ttgccaaata caatagtgtt
     7381 aataaatatg gtttgaatgc ttcaacatat ttattaggtg gctgtttact tttagatttc
     7441 atattgcacc tcttaaagtt cttagtaaaa acgcctttat aaagaccgtt caatataaaa
     7501 tacgttttaa aatttgtttt ttacaattca ttatatcgat attcataatg aaattcaatt
     7561 ttaattttat agattcaaca tagtaattgg ttgtcatcac tcaattattg taaatgatac
     7621 actttgttga gaacatcatt cattttaaag gttatttaaa caataaacaa ttacagtcta
     7681 tataacaatt ttgttatata cgtcaaaatc aaataaactc atcacattaa tatgacgagt
     7741 ttataatgtt attgaattat catcagcgca aatatataca ttcgcaagtc aagcataaca
     7801 tatttaacaa ttgctttgct tgttttacca atgattaaaa accatactta ttttcaattt
     7861 actggagtat gtggtacctg atttgtcata accgcttcta tattattaat acataattga
     7921 atcatattgt cccgtgttgt tactgatgcg ctaccaatgt gtggtgtaat cagaacatta
     7981 tcacgtccca ttaatggatg tgtatgatca atcggttcat ttgctaatac atctaaacca
     8041 catgctaaaa tttctttatt gtctagtgca tcgattaatg ctgtttcatc tacaatttgt
     8101 cctctaccga tattaataaa aattgcatca tttttcattt gttcaaatgc ttcagcatta
     8161 aatttatgat gtgtttcttt tgtaagtggc gctgtacaga tgataaaatc actttctgct
     8221 aacaacgttt caaaagaaac atatgttgca ttaaagtccg cctctgcatc tttatgtctt
     8281 gatcgattat gataaagaat attagtatta aacccttgca accttcttgc aaaagcttta
     8341 ccaatatctc ccataccata tattccaata gttgaattga agacatcttt acctgacaat
     8401 aaataaggac cccagctttg ccatgcatct gcttctacat atttttcagc ttctacaata
     8461 cggcgtgcta tagcaagcat taatgtaaat cctaattctg cagttgtttc agtaagtaca
     8521 tttggtgtat tcgtgacaac cacgttattc gctgttgcac tttcaacatc gatgttgtca
     8581 tatcctacag ccatattcgc aattactttt aaatttggtg attgcgctaa aatttctgca
     8641 tcgatctgtt cacttaatgt aataaaacaa gcatctgcgt cacgaagtgc tgtcacaaat
     8701 tgatctttag gcataggcac taatgatttt tgccacataa caacgtcacc aagtttactt
     8761 aattgttgat aaaatttatc tggaattttc ctcgaaacaa ctatttttac caaatcaatt
     8821 cacctacttt ataaaatgac tatatctatt tttctaattc agctattgct tcatttaaat
     8881 ctttaaaaga atacgttggt ggcacatttt tattttgcac atcttctaac gtagatacac
     8941 ctgtttgtac atgaatcgta tccataccta cgttaatacc agacataata tcggtatcgt
     9001 acaaatcgcc taccatagca acttcggatt tatctaatcc taaaatttct aatgctttaa
     9061 ccataataat cggttctggt ttaccaataa attgtggcga tacacctgtc gatacactta
     9121 caacacttgt aatagcacca ttaccaggta ataaaccacg ctctttagga attgatacat
     9181 caggatttgt agaaataaat gttgcaccat ttcttacacc taacgttgca atcgcaagct
     9241 tttcatatgt aacttgttcg tcaagtccaa taactacata atcaacatgc tcgtcatttt
     9301 taatgacaag tcccgcttcg gttaacgcag tatttaaacc actcccacct aacatatata
     9361 ctgatgctcc tggtgattgt tctgaaatat aatcagcagt ggctaacgct gacgttacaa
     9421 cctcttctgg tttagcatca atgtgcattt cacgtaattt ttcagttact tgctcaggtg
     9481 tttttgttga attattagtt acgtataaat gaggaatgcc ttttacattt aaataatcga
     9541 tgaattgttt tgctccatca atctcatctg ttcccatata cattgtgcca tctaaatcga
     9601 ttaaatacgc tttatactgt ttcattattg attttctcct ttaccaaaag ctgttactgg
     9661 tacattttct tgatgtaaaa atgtcgttac ttctgtaata aattgtttgt aataaggtaa
     9721 tgcgtcatca aacattggca tgataatctt aatatctaag gctgtgtaat catatgtaaa
     9781 ttgtttacga ataccgacag ttttattaat cgcctgctgt gtttctttag taataacatt
     9841 ttctagttct aatatatcaa tcacatcttt ataattacca ggatccctta aaataaatgc
     9901 atcgataatc atattcccta tatctaccga tgattctatc aacatttgag caatgcgttc
     9961 aaatgcataa tgattgtgct tgctctcatg ataatcatca gttaatgctt gtaaataggc
    10021 taatttctga gttagtttat ctttgtctac aaaatacatt ccttgtcacc tctcacttat
    10081 atattatcat aattttgcag tatacttcat ttgtcgttat actaacttta ctacttttaa
    10141 aaggagtcgc tacaaaatga ttgatatgta tttatatgat gacaacgaag agagtcaagt
    10201 tcagtttgtt ggttttgttg gcgagcatag tcgatatgat ttgatgttag tccatacaaa
    10261 tagacattat ggtaagacac tcgtacttaa tatgcaaaca aataaattcg gtattatcgg
    10321 tactgatgat ttaaaagaag aaggctatat cgctcatatt ttaggtgtaa atgctgaaga
    10381 aggcgatgaa attactgagt atttaaatga agtcattcat taaagataaa aacccctaac
    10441 tagattacag gtccatgctt gaattgcatg attcccattt tcaatctagc taagggtttt
    10501 attaatgttc agtatcttga atggttggct taatcttcac atcttgaagt ttattgtcgt
    10561 catctatcac atcagacgga tgaacttctt cgattaactt tttaacctct tgtttagaca
    10621 aatgtcttaa tacaaagtaa ggacaaccaa agttacaata ttcaaatatg taatcttgaa
    10681 tatttgaaaa acgtttactc atctctgctt ttttattaga atctttgtaa aaacctttta
    10741 atcgtaattg atcgtaacca tagtcaccaa ctatgtaatc atatttatct aaaatatctg
    10801 aatacctagc aataaattgt tcttcattaa aacattcgcg ataattttct attaattcaa
    10861 agtaatgttg atctactttt atcaaattct tcacctatct atgacaatga aaggctcagt
    10921 ttatactttt agtgtttcat aaaaataatg attacttcta tgacacgttc gttttgtaca
    10981 aactaagcct ttatgcctta ttaatggtta aatattaact atttaactgt gcctcacctt
    11041 ggcgttgctt ttctttagca gcttcattta cttgctcatc cgcatgataa gaactacgta
    11101 ctaaaggtcc agcttggcaa tgtttaaacc ctttatccat tgccactttt cttaatttac
    11161 caaactctaa aggcgtgtaa tatttttgaa cctttaaatg tttacgtgaa ggttgtaaat
    11221 attgaccaat cgttaaaata tctacatcat tcgcacgtaa atcatccatc gtttcataaa
    11281 tttcttctat agtttcacct aatccaacca taatacttga tttagttggg atatccggtt
    11341 gtaattcttt tgaacgacgt aaaaactcta atgttctgtc gtaagtcgca cgcgcacgaa
    11401 ctctcggtgt taagcgacga acagtttcaa tattatggtt taaaatgtca ggtcttgacg
    11461 ccattaatgt ttctaacgca tcatagtccc cgcccatatc tgatggtaaa atttcaatcg
    11521 ttgtaaatgg atttctttct ctaactttac gtactgtctc agcataaaca tttgaaccag
    11581 catctcttaa atcatcacgc gcaacagcag tgataacaac gtgtttcaaa ttcattaatt
    11641 caactgattc agctacacgt tcaggctcat ttaaatcaag ttcattaggt aaacctgtct
    11701 taaccgcaca aaaacgacaa gctcttgtac atacggcacc taaaatcata aatgtcgctg
    11761 tacgacgtgc accccaacat tcatgtatat taggacattt agcttcttca catacagtat
    11821 taagattttt ttccctcatc atcttcttaa gtcctgtata gttttcgttg gtatttaatt
    11881 ttattttcaa ccaatccggt ttacgtaata tttcctcgtt ttttgtcgcc ataacaacac
    11941 ataccctcct gttaagtgta tctttaaaca ttatatcgaa attcatgaca aatgaaaatc
    12001 caataatctt actttttaat attccaataa tttttcttta aatatatctc ttaaaaactc
    12061 tggcattaaa tactcttttg gtaattcttt caatgttggg aaataacgac cgaacgtata
    12121 catatctatc gtacctagta catattcttt gtcgtcttcg atttcttctc cattcagata
    12181 gtaacgccct gtagaatgaa tgtaccctaa attataaaga atatatcctc caaatatatt
    12241 ccctctgaaa cccaaacctt gtgcatgttc atacatatat tcttgtttat tacttttagc
    12301 tataatctct tttaatttca caccaaaaag tctaaccctt accatattaa tcggatgcgg
    12361 taacatttga tgaatgtcat attctgtcac ttcatctttt acaatatctt taacgagtaa
    12421 gccagcattg atgatggcac attgtgcatg tgtatactca caaacacttt gagctaataa
    12481 gtatgcagct tcagttatgt gattcatact acgcttaagc actactggat gttgaattac
    12541 tgaattggac attaacgttt ttccttcttc ttcaaatgaa gtttcaactt caggtaatgt
    12601 ttctaaagga ataatctttg cagttttatg tactacttta tgtgcctcaa aagttaaatt
    12661 aacctctcca agataattac catactttcc agctgccgcc atcagtacac cattattgat
    12721 ttcaccatgt tcaaaataat gatgcgtatg actaccaaaa atgacatcaa tttcaggcaa
    12781 ttcttggcat aatgtttcat cgaagaaaat gccacaatga cttagcacga ttaatacatc
    12841 aaatttacct cgttgaagtt caatttcttc ttttatagat tcaagtggat cggtaacaat
    12901 ccaatttagt gcacgataaa atggggtaaa aggtgctgtc gctgcaacga atagtatttt
    12961 cacaccgtct atgtccttaa tataagaaga aacgatatta tttggtaaat gacctgattc
    13021 atctataaca ttgctacatg tcactataaa ttttgcttcg tcgtaaaggt gatttaaagc
    13081 ttcgtgtgaa atggtcatcc cttcattatt accgattgtt gcaacatcac attttgcttc
    13141 atttagtaat gccacattct ttttacctaa agttgcttca gttataggtg cggataaatc
    13201 tacatgatca cctagatcaa catataaaga aggatgatta agtcgtggcc gatgttctgc
    13261 catatatgct ttaatgcgtt cgtattcatg taaatgacta tgaatatcgt tcgtatgata
    13321 aattgtaagc ctcaaattga cgtcctcctt ttaaagttgt attaaatcaa cgcacgaata
    13381 attaaatata tacccattaa caacattact gttctcaata atgttactac cgtatctgac
    13441 ttaattgatt gattcacttt gacaccgatt ttcgcaccaa aataactaga aataattaaa
    13501 atgattgcat aaccccaagc tacgtgacct tgagcaatgt gccctataga actcatgaca
    13561 cttgaaaaga aaatcatcat catacttgtt ccaacagcta catgaggtgg aaatctaaat
    13621 acaataagca ttagtggcgt cattagtgcg ccacctccaa taccaaataa acctgtcaat
    13681 ataccaatta aaaacgttgt aataaaagca aacaatggtg gaacactata atgatatgtt
    13741 ttacctttag cgtctacata agtcttttca tacttgggtt tatcgaaaat tttaaacggt
    13801 ttaatcttat ttcttaccat taataaaatg gctacgaaaa ttaaaaagat accaaaatat
    13861 aaattaaatg actcaaatgt taaatatcta ctaatgaagg acccaagcaa tgaacctggt
    13921 aatagtccaa ataaaaagat ggaaccattt ttaatatcaa cttgttttgt ttttaaatat
    13981 ccaagtgatg aagaaagtcc tgtcacaatt agaatgactg aagacgtccc tatcgctact
    14041 tgtgttgtaa tattatgtag taatccatgt tcaacaccga ggtaaaccat tgttggaacg
    14101 ataataatac cgcctccaat gcctacgata gaccctataa tcgctgacaa acctccgatt
    14161 aaaactaata atgtaattgt taataacata gtcgtcgcct cttaaaataa ttttaattgt
    14221 tgtggtgcta acccttcata ttctatattc aataatcgct gatatgtttt ggcattattt
    14281 gctgcatgac caccagaatt attgttaaat atgacgtaaa ctttcttagc tttttgtgct
    14341 aatatttgtg ccttttgtgc caagtctatt aattcttgct cattataatc atataaatag
    14401 cgtacatcgc gccattcttg atctgacata tctttcttag tccaaccgta atgattacgt
    14461 ccatgataac gtacaaacgc aatttcattt gtgattcgat tgactaaagg tacactgcca
    14521 tctttcactt gtggttcatc aactactgca tgaatgattt gatgttctgt taaaaatgcc
    14581 aatgtttgtt ctttaaatgc atcactaaac catgattgat gcctaaattc tacacacatt
    14641 ggaaatgctt gtaattgctg tcttacatac aaaatatatt tgatattttg tgcattgcag
    14701 tcaaaccatg gcggaaattg aaccaatacc attgctaatt ttttctgtgt atgtaagggc
    14761 tctaacatat tcttaaattg atcaaatagt tcttgccttg tatctgcaaa tgttttgtag
    14821 tctgcatgca atgtgagtgc ttgatgaatt ttgaccacaa attcaaatgt atcaggcgtt
    14881 tcttttatcc atttcaatat atttctttcc ggttgtatcg catagtatgt cgcatctaat
    14941 tcgacaaccg gaaaatgtcc agcatatgtt ttaagtttat cggtttggcg ttctaaatct
    15001 tcatataatg aatagtgatc accccaacct gttaatccga tgtttatcat atatatcacc
    15061 aatgtcatca taccatataa cttttatcat aatcatttca gcgaacttta gtttgatgtt
    15121 tttgcttgat taaaatcttt cggcgatctt cagcttgatg ttttcgtttg attaaattgg
    15181 taaaatagaa acgcaatcca caaaaatggc aagcactaaa ataatgtttg ggggtgcttg
    15241 tgcttttgtg gattgcggtc gattatttat attgcatgat ttgattaatt tgattgatta
    15301 tattggacat gatggtgttg gcgggatgcg ttgttgctag tcgcgggctt tgtccactcc
    15361 acatatgtat taactctttg tcgccgatgt ttgctgcggc ttttcttatg ctacttgtta
    15421 gctcattttg tattggataa tctgggatat cgccttcgta ttgggacatt tcttcgataa
    15481 acctattgtt gataccgcgt gcaagctttc cactaaacgc ttttgtaatg actgtatctg
    15541 tttctttact atttataatt gcatctcgca gtagttctga tgcattactg tcttgtgatg
    15601 ttaaaaatgc ggtgcccatt tgtacccctt ctgcacctaa gacaatactt gccaaaactc
    15661 ctctaccatc cataattcca ccagcggcaa tgaccggaat tgaaacgaca tctacaattt
    15721 gtggcactaa agatattgtt ccaaccatag gtaattgatt tttaggtttt aaaaatgaac
    15781 cacgatgtcc acctgcttca ctaccttgag caacgatagc atccataccc gctttttcat
    15841 tcgcaatagc ttcatcaaca cttgttgctg tacctataag tttgacattc gctgctttca
    15901 acctgcttat aatctgttcg cttggaattc caaaagtaaa acaacataca ggcacttgct
    15961 ttttaattat cgtatcaata tgacacttaa attgttgttc ttcggtaatt tttacaaccg
    16021 gctcttctaa atgtaatgcg cgtcgataag gttttaacca tgcattcata ttttcaattt
    16081 gactactggt atatgattgt tgacttggta caaagacatt tacgccaaaa gaatttgacg
    16141 ttaattggcg tacataatct atttcatctt ccaattgctg cgtattaaag taacctgcgc
    16201 ctattgtgcc taacccacca ctgttactta ctgatgcaac taatttcggt gtcgtacttc
    16261 ctgccatacc tgcttgtata attggatatt caatacttaa catttgagta agtcgattct
    16321 tattccacat agctgttcgc tccttatata gatacgttgc gatttttccg ttgttgaaat
    16381 tgaatttgct gttgagaaag tttttctttt tcctttttat ccatctcatc ttcaatttcc
    16441 atacctaata attcttcaat taagtcttca tgtgacacta tcgcttcagt accaccaaat
    16501 tcgtccaaca caattgctaa atgttttcta gaaatagtca tcttacgtaa tacccattca
    16561 gctttattgt gttcattcac aaataatggc ttagctgaat agtttgtaat ttgattttct
    16621 tttttattac tccaagccaa cagatattta gaatgaaaca ccccaataat gttatcaata
    16681 tctccctcgt acactggata tctagtgtat ggcttattca taaccgtttc ataaacttct
    16741 tcgtatgtcg catttgaagc aaatgccgtc acattaattc taggtgttgt atctacatct
    16801 tttactttta aattttcaaa attaatgaca ccttccaacc tactcgtctc aatttcattt
    16861 aaagcacctt catgtccagc aattgctaac attgttttaa attcttcttt tgaaaattga
    16921 tgttcttgag gttggccctt agataaactt cgattaatac tgtccgtcaa cttatttaaa
    16981 agtaatgtga taggacggaa cacaatgaca caaatattaa taattggata tacaagcctt
    17041 gttattttat ctggaaatgt tgcagcgaca gacttgggaa tcacttcgga gatcaaaatg
    17101 ataacaactg ttaaaacagc tgatgcaata ccaacgctaa tcccccaacg taaagccata
    17161 attgtaacaa gtgttggtaa taaaatattc gcgacattat tcccaattag aatcgttgta
    17221 ataaactcac ttggtttttc aagtaacttt acaatgcctt ttgctttttt atcacctttg
    17281 tcagcttcag ttttaaattt tgttttattg gcagccgtta atgccgtctc gcttcctgaa
    17341 aagaaaaacg aaataaatat caatataatt atggcaatga tcacgcgtgt agtctcctta
    17401 ttgtcatatc ttatttttat tgtagttact taattcccga cgttctcgtt taa
//
LOCUS       DAKTIP010000036        17180 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAKTIP010000036 DAKTIP010000000
VERSION     DAKTIP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.036"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            164..805
                     /locus_tag="O0305_000948"
     CDS             164..805
                     /locus_tag="O0305_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCY0274101.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(975..2030)
                     /locus_tag="O0305_000949"
     CDS             complement(975..2030)
                     /locus_tag="O0305_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCY0274102.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            2348..3418
                     /locus_tag="O0305_000950"
     CDS             2348..3418
                     /locus_tag="O0305_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCY0274103.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            3614..4375
                     /locus_tag="O0305_000951"
     CDS             3614..4375
                     /locus_tag="O0305_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCY0274104.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            4393..4887
                     /locus_tag="O0305_000952"
     CDS             4393..4887
                     /locus_tag="O0305_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCY0274105.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            4901..6127
                     /gene="pepT"
                     /locus_tag="O0305_000953"
     CDS             4901..6127
                     /gene="pepT"
                     /locus_tag="O0305_000953"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCY0274106.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(6316..7440)
                     /locus_tag="O0305_000954"
     CDS             complement(6316..7440)
                     /locus_tag="O0305_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0274107.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAYQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(7865..8185)
                     /gene="ytxJ"
                     /locus_tag="O0305_000955"
     CDS             complement(7865..8185)
                     /gene="ytxJ"
                     /locus_tag="O0305_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCY0274108.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            complement(8339..9217)
                     /locus_tag="O0305_000956"
     CDS             complement(8339..9217)
                     /locus_tag="O0305_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCY0274109.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            9337..9855
                     /locus_tag="O0305_000957"
     CDS             9337..9855
                     /locus_tag="O0305_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCY0274110.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QQYFEKLCKKYL"
     gene            9982..10905
                     /locus_tag="O0305_000958"
     CDS             9982..10905
                     /locus_tag="O0305_000958"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCY0274111.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            10923..11237
                     /locus_tag="O0305_000959"
     CDS             10923..11237
                     /locus_tag="O0305_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274112.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(11554..12582)
                     /locus_tag="O0305_000960"
     CDS             complement(11554..12582)
                     /locus_tag="O0305_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0274113.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(12701..13462)
                     /locus_tag="O0305_000961"
     CDS             complement(12701..13462)
                     /locus_tag="O0305_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274114.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFENGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            complement(13459..14415)
                     /locus_tag="O0305_000962"
     CDS             complement(13459..14415)
                     /locus_tag="O0305_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0274115.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            complement(14402..15373)
                     /locus_tag="O0305_000963"
     CDS             complement(14402..15373)
                     /locus_tag="O0305_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274116.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            complement(15412..15567)
                     /locus_tag="O0305_000964"
     CDS             complement(15412..15567)
                     /locus_tag="O0305_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274117.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            complement(16081..17052)
                     /gene="nrdF"
                     /locus_tag="O0305_000965"
     CDS             complement(16081..17052)
                     /gene="nrdF"
                     /locus_tag="O0305_000965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCY0274118.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
BASE COUNT         5770 a         2943 c         2494 g         5973 t
ORIGIN      
        1 gtcatcacag caattttcat agtcgtcctc ctaaaatata ctcatagcaa tgatacaata
       61 tttcagttaa aaagaaaatg gcaatcaaac gatttatagt catgtcattt tcagtgtata
      121 atgaagatta aataatctta agcttatgaa ttaaggagtt attatgacac aaaatatcat
      181 tactgttaaa aaagaacaca ttattgaaaa tgtgattagt aaatcaagat tcattgctca
      241 tatcaaacct gttcaaaatg aagatgaagc taaagcattt attgctgcaa taaaaaaaga
      301 acataaagat gccactcata attgttctgc atatactatt ggacctgaaa tgaatattca
      361 aaaagcgaat gatgatggtg aaccgacagg aactgccggt gtcccaatgt tagacatatt
      421 aaaaaagctc gatgttcata atgcgtgtgt cgtagtaaca agatattttg gtggtattaa
      481 attaggtggt ggcggtttaa ttcgcgccta tagcggtgct gttagagacg tcatttatga
      541 tatcgggcgt gtcgaactac gagaagcagt accatgtata gtaacactga attatgatca
      601 aactggtaaa tttgaatatg aattagcttc aactcatttt atgttaagaa accaaactta
      661 cactgataag gtaagctact atatcgatgt cgttaaaagt gactatgaag cgtttattaa
      721 ctttttaaat caaatgactg caggaaattt cgatttgact gaagaaactc caaagcagtt
      781 acctttcgat ataccaactg aataaaaata aaaagcacta tcataagacg gtaaatatca
      841 attgcgtatt attaaacgac aattgaagta cacgtttatg gcagtgcttt ttatatttga
      901 aatttaatac aaaaaaattg taaagagtaa agccatataa tacaaattat cccatacagc
      961 tatgctttca ttccctattc ctctttatga gatgacttac gactaattaa atttaatatt
     1021 ggtcggtagt tgttatctat taatcctgta aattcaacaa ttaattcaat actaaatatg
     1081 attaatacaa acattaatac aacacctaat ggtggcgata catacaaaat aatgctcgaa
     1141 agactaaata aaatagagat tgaatagatt aataatactg tttgtctatg tgtgtagcct
     1201 aaagctaata gtttatgatg caaatgcgat ttatcagctt gcattatatg ctgccctttt
     1261 ttcacacgtc gaatcattgc gaacaaagta tcaatgaatg gaactgctaa gataacaatt
     1321 gggaagaaca atgcaataat tgtaatattt ttgaatccga gtaaagaaag gaatccgatg
     1381 ataaatccaa tcattaaagc cccactatca cctaaaaata ttttggcagg atggaaattg
     1441 taaaataaaa acccaattaa agagcctaat aaaacacaac aaatcatcgt tatgaaaata
     1501 ttagcttgta aaattgcaat gaaccctatt gtaatgagtc cgattgcaga aacacccgac
     1561 gccaaaccat cgagtccatc aattaagtta attgcatttg taatagcaac aatccaaatc
     1621 acagtaattg gaatactaag aaatccaaaa tgaatcgttg tacccattgg caacgaaata
     1681 aaatcaatag tcacaccata aaaagcaact actaaggcag cggcaatttg accagccaat
     1741 tttatatacg gtttcaaatc gtagatatca tctacaagcc caagtacgta cataataatc
     1801 gcaccaataa taagtggttt gatttctgtt tcaataggat gaccaatcca aatacctatt
     1861 aaaaatgaaa agagaatcac tgtaccaccc ataactgaaa taggtttagt gtgtactttt
     1921 ctaaaatttg gtttatcaac taaatttaat cttttcgata ttgcaataac aattggtgtt
     1981 atcgtcaaac tgacaatcat tgttactgca actagtaata atgtaaccat ttattcacct
     2041 tcatcgatat taattgtttt taacgttata tttatttaat attgtcttta tagaatgata
     2101 tatctatttt ctacgtatat cttatagctt tattataaat taattcgttc aatcaatgat
     2161 atcatattta caactatacg attcatttct tatatatcgc ctattattca ttttttcatc
     2221 atatcattta aggcaacatt ttaacatcaa agtctatatc atacattaat tttatatgta
     2281 aactaatgac aaattgtaag ttttcactta caatagctag tggtcaatta aaggagtcga
     2341 ttcagacatg ttcgaagcat ttatatacaa tatatctgtt atagtcgctg gaatctattt
     2401 attccatcgc ttacagtatt cagaaaacaa acgtatggtt ttttctaagg cttatgtcac
     2461 tgttttaatg acaatcgtat cattattatt atctgtctac cctatccctt atcgtgaaga
     2521 ttatttaatt cacttaacat tcgttccttt attgttttta ggtagattta caaatatggt
     2581 ctatacatta tctgccacag taattgtagc aattgttgaa attgtagtat ttaacaactc
     2641 gattatgtac ggtgttacgt tgattgttat tgctgcagta acaagtgcta ttggaccatt
     2701 tttaaaacaa aatgatgtgc tatcattatt aatacttaat gttgttacaa taatcatttt
     2761 atttggtgtc gcgttagtga gtcctattta cacactatcg gaagttatca ttttaatacc
     2821 aatttcatta attatcactt tagcttcagc gataacattt gtcgatattt ggcatttctt
     2881 ctcactcgta aatcgttatg aaaacgaaga taaatatgat tacttaacag gcttaggtaa
     2941 tgtaaaagaa tttgatagac atttaaatga aatttcacga aaagctgaaa aagaacatca
     3001 aagtatcgca ttattattaa tcgatatcga tggatttaaa gatgtcaatg atacctactc
     3061 acacaaatca ggtgatgctg tattaaaaca aatgtctcaa ttacttaaaa actatgtgcc
     3121 gaatcaattt aaaattttta gaaatggtgg cgaagagttc tcagttgtca ttcacaatta
     3181 ttcacttgat caaagtgtta aattagctga aaatattcgt tcgggtgttg aaaaatcatc
     3241 attccattta ccaaacaaag aagttattaa attgtcagtg tctatcggtg ttggttattt
     3301 aacagatgac gatccaaaat cacagcgtaa agtgtttaaa gatgcagacg atatggtgca
     3361 tgtagccaaa aatcaagggc gaaacaaagt aatgtttaac cctattatca atttataaca
     3421 acgacaaaac tgttaatcct ttgctattat taaatcatag tataatagca aagattaaca
     3481 gtttttctta tttaaatttg aaccatacaa gttcatcata ctaacacaaa gtctcactat
     3541 tgaaagttgt aacaacaagt tgtaaacttc aattgttcaa ataaataaca catatcattg
     3601 gaggtgaatt gatatgccag attcaatcac aattatagat gaaaacaaag tgattgatgt
     3661 tgtattaatt gcaggtagaa ttttacttga atcaggtgct gaaacatatc gagttgaaga
     3721 tacaatgaac cgtatcgcac atagttatgg tcttcataat acatatagtt ttgtcagttc
     3781 aactgcaatt attttttcat taaacgatcg aacaagtaca agattaattc gtgtacaaga
     3841 gcgtacaaca gatttagaga aaatcgcttt aacgaatagt ctttcacgta aaatatcaaa
     3901 taaagaactc acaattgatg aagctaaatc tgaatttatt catttacagc atgcatcatt
     3961 acaatattct tttttaacga atttctttgc agctgccatt gcatgtggct tcttcctatt
     4021 tatgtttggt ggtgtcgcat cagattgttg gattgcagtc attgctggcg gatccgcatt
     4081 tttaacattc agctttgtgc aacgctatat acaaattaaa tttttctcag agtttgttgc
     4141 agccgctgtg gtcatatcca ttgcagccac atttactaaa ttaggcattg caaccaatca
     4201 agatatcatt actatagcca gtgtcatgcc acttgtccct ggtatattaa ttaccaatgc
     4261 cattcgagat ttattagcgg gtgaattact tgcaggtatg tctcgcggtg ttgaagctgc
     4321 attaacagca ttcgcaatcg gtgctggtgt cgcaatcgtt ttattaatca tttaaggaag
     4381 gactgttaaa gcatgctatt ttatttattt cattttacaa tcagctttat atcaacagta
     4441 cttttctcta tcattttcaa tgcacccaaa cgcctcttag tagcatgtgg atttgtgggt
     4501 gccattgcat ggacgattta ccaattaacg gtagatttag agtttggaaa agttggcgct
     4561 tcatttttgg gaagcttaat tttaggctta atgagtcata ctatgagtcg cagatataaa
     4621 cgaccggtaa ttatattcat agtgccaggc attataccat tagtacctgg tggtgcagct
     4681 tatcaagcga ctcgtttttt agtatcaaat gattatacaa gtgctgtaaa tacattttta
     4741 gaagttacac tgatttcagg tgcgattgct ttcggtatat tagtttctga aattctatat
     4801 tacctataca cacgtatcaa acaactgtat ggtaaaatca aaggtaagac atataaaaaa
     4861 tcttacaaca tgaataatag agtttaaggg gtcgaatata atgaagaacc aattgataga
     4921 tagattaaca agatatacga caattgatac gcaatctgat ccaaaatcta caacaacacc
     4981 ttcaactgaa aagcaatggg atttgttaca tttattagaa aaagaattac aacaattagg
     5041 attgccaact gatttagatg aaaatggcta cttatttgct acattagaaa gtaacatcga
     5101 tgtcgacgtc ccaacagttg gatttttggc gcatgttgat acatcacctg atttcaatgc
     5161 ttcaaatgtt aaaccgcaaa ttattgaaaa ctatgatggt aagccataca aactaggcaa
     5221 tactaaacgt gtcttagatc ctaaagtatt tccagaactt aacagtttag ttggtcatac
     5281 attaatggta actgatggta catcgttact aggtgcagac gataaagcgg gtattgtgga
     5341 aattatggaa gctatttgtt atttacaaga acatccagaa attaaacatg gtaccattcg
     5401 cattggattt acaccagacg aagaaatcgg tcgtggtcca cataaatttg atgttgaccg
     5461 cttcaatgct gattttgctt atactatgga tggtagtcaa tatggagaat tacaatatga
     5521 aagctttaac gctgctgaag cggttattac atgccacggt gtaaatgttc atcctggttc
     5581 agctaaaaat gcaatggtaa acgcaatacg tttaggtgaa caattcgata gtttgctacc
     5641 tgatagtgaa gttccggagc gaacagaagg atacgaaggc ttttatcact taatgaactt
     5701 tgaaggaact gttgaaaaag caactttgca atacattatt cgtgatcatg ataaaaaaca
     5761 attcgaattg cgtaagaaac gtattttaga aatacgtgac gatatcaatg cccattttga
     5821 aaattatcca gttaaagttg atatatcgga tcaatatttc aatatggcag aaaaaatatt
     5881 accattgcct catattattg atatacctaa acgtgtcttt gccaaattag atattccagc
     5941 aaacactgaa cctattcgcg gtggtacaga tggttcacaa ttgtcattta tggggttacc
     6001 aactccaaac atctttacag gttgcggtaa cttccacggc ccatatgaat atgcatccat
     6061 tgatgttatg gaaaaagcag tacaagtaat cattggaatt gtagaagata tcgctgaaaa
     6121 tcactaaaaa ccaacgatta tatactagtt caactcaggc ttaattaata tataaaaatc
     6181 acgctaacat tcagtgcaat gatagcgtga tttttcatat tttttacgta tactttcgat
     6241 cttctaattc gttttgctaa ccatcataaa tgttattaac catcatgaca gcatcaccaa
     6301 tttagtcctt ctttactaaa cctcaacatt aaaatgtgtt ttcaacagtt ttaaagactg
     6361 aaccgtataa tgcctaattt gtaaccccat tttaaagtca gtataagttt ctggcatatc
     6421 gataaatgta ttaaacattg ctgtaacccc ttgtgattca aataaatcaa acttttcagc
     6481 agttgcacaa attgcaatgg caaccttttg atgtttatga caaagttctg caatacgcaa
     6541 tgttgtcgtt tctagcaact gatcattttc atttaatcct tctccaaaaa taattaaatc
     6601 cgcttgttca actaaatttt ctaaatgtgt tagttggtct actaatgcat gactggttaa
     6661 tatttcagct tgatacagtc cattcaagac tgctgcaatt ccaccaccag caccaccacg
     6721 ttcaactgga ccaattgcaa ttttcaattc acttttaaat aactcactaa aataccaaat
     6781 taaattatcg atttctgctg cttgattatg attcaactga tacgcatcat aagtttgcat
     6841 gatttcactt tgcttaccat ataatcgact tgaaaaatcc gacattactt gaattcttgc
     6901 tgtttccatt ttagggtgta agttcgacat atccatacga cgaatatatt taattacacc
     6961 agcaccttgt ctcatatcta cgacacgccc ttcgtcatca tagaattgag cacctaatgc
     7021 ttgtaacata cctgcaccag catcaaaact atcaatccca cctagtgaaa ttacaacatg
     7081 ttttgcgtcg ttatctaatg catgtttaat catttctcct aaaccataac ttgttcgttc
     7141 aacaattggt tttttacctt ttaaaaataa attcccctcg ataacggtca tccctgtatc
     7201 agtttgtccg taaacacctt caacttcatt catatctgca tcatgtactg gtatacgata
     7261 cttttgccca gattgccata aaaatacaga atctaataat tcatgtcttc cattaaacaa
     7321 tggtacttga actacatctg cagtttcaat ttggcttgca actgcctctt caacatatct
     7381 attagcttga taacttgaaa taattccatg aaactcatcc atggctacta atactttcat
     7441 aaccttgacc accttacttt attatttgtt gtgatacgtt ttttattcat aaaagattaa
     7501 tcttaaattt tagattttat ctcgtgatta tatgcgaaca tttaacatga catgattatt
     7561 aattattcca gtaaataaca atatgcttct tttggtttag catatatttc ctattaaact
     7621 aacttacaaa tcatgtttaa attttcttat tggattcgtc gttttccaaa atattttcaa
     7681 taaaaatact cgaatatatt atacatatgt ttgtaaaaaa gtactaactt aactgcttct
     7741 aaaggtgaca taaaataaaa ggtcagaaca tcattatagc caaactactc tgattcacac
     7801 gaatttaagt tgtgctattc acgtctagta gtgtaaggca aaatgttcca accctatagt
     7861 ttcattattc ttctgcttgt gctaatgacg acacattgat atcaccgtgg tctcgattcc
     7921 aaaccatttc accatttaca aaataaaatg cttgaggtga ttcatgttta acgttcgttt
     7981 ttttagcaat ataatctgac aaatcgcgtt cttgttggac aatcaaataa taaccgtcca
     8041 tatcgcgttc atataaaaat ttattaaatt gatcgtacgc atttgccgat attggacaag
     8101 tttcactatg ttttaataca aaaacatatt tattttcctc aataacctgt tcaaattggt
     8161 caattgaact tagctttata gccactctat tcacctctaa aaatatttat cacatttaaa
     8221 tagcatctgc gtgacatttt tatacatata gttaatatca atgtttacca ttatacacat
     8281 agtaagttga catgttaacc ataaattcaa aatttttaac tttgaaaaat gattatagtt
     8341 atttttcttc taatttttca agcttttttt caaaggcttt atcgccgtga gttatatcta
     8401 tacccttttt cggcaactta tctacatcga cttttgataa cttctcacta acctttattg
     8461 tttcaatacg tgtattataa tagttctgca gactgtaata catttctatt gcgtttttcc
     8521 ttgcattatt aacatgctta tcactaatat tagtttggtt aatatcggta atttgctttt
     8581 caatcattgg cataatgtga tttttaatcg cgccttttac ttcattttct ttactatcat
     8641 ctaggttctt cttcgcagta tctcttaacg ctttattatt atgttctaat tttgtcgtaa
     8701 gattagttgc ttcactttta ttatcagcta atttaatttc tgattcaact ttgtatctat
     8761 ttttttcaaa ttgtttcgta taatctaaaa tgtcttcgtt atacttgata gattgattac
     8821 ataaacctat gaattttttt aattgatata tcgcatcctt cttctcattt gcaagcgtca
     8881 tatattcttt ttttaatttc ttaactttca ttgtatcatc aggcaaattt tttgctgact
     8941 tataatatgc ttcaaattta ggtatgagat ggtttttaac atcttcttgt agtgccttaa
     9001 attctttact atttttatca gttgtatcag ttttacttag atgatcaatt tctttcaaat
     9061 gtatattatc catgacatgt tgtaattctt tttgtttttg ctcaacatct ttaaattgct
     9121 tagtaaattg atgttgtgcc tctttttctt tattaccaca agcacttatt actacaaata
     9181 aaatcgctaa aacagcgata ataacaattt ttttcatgat tgcactcctt aacagattca
     9241 ttatacaaaa acatgtttat tatgaaaatg aattatgtta tagtattcca gactttatcg
     9301 tgattaaatt aattttcaat atcaaaggag tttatcatgt attcaaatgt tcaaccattc
     9361 attaatagtg atttaactaa ttgttttcaa tcacaataca ctgaaattga atcattatta
     9421 ttcaacctct tagacagccc agtaaaatat acaaaacata ttggcggtac gcatcatttt
     9481 aaatattcaa cagagccaat tttagatatt ttagtaggtg tagaaaattt gcatgatatt
     9541 acatccttag atgaaaaacg cttaaattat gtagggtttt atcgtttgca tcataaatat
     9601 actaagaaag ttatgatggc gaagtttaac aattttcatg aactaaaaca acaggtgcgt
     9661 ctacatatca ttcaaatgaa tacccaaaca tttgaatctt atcttgcact tgattacctc
     9721 ttatcaacta atgatgatat tgctttgcaa ttcaatacaa aaaaacacca aatattgcaa
     9781 catagcttaa caattagaca atatgagatg caaaagcaac aatactttga aaaactatgt
     9841 aaaaaatact tataacgaca ataatgccgt attttataat tatactaatt ctaatacttt
     9901 cttttcaatt ttcgcaaatg aattttaaaa ttggtataat actatatgat attaaagaca
     9961 tgagaaagga tgtactgaga agtgataaat aaagacatct atcaagcttt acaacaactt
    10021 atcccaaatg aaaaaattaa agttgatgaa cctttaaaac gatacactta tactaaaaca
    10081 ggtggtaatg ccgactttta cattacccct actaaaaatg aagaagtaca agcagttgtt
    10141 aaatatgcct atcaaaatga gattcctgtt acatatttag gaaatggctc aaatattatt
    10201 atccgtgaag gtggtattcg cggtattgta attagtttat tatcactaga tcatatcgaa
    10261 gtatctgatg atgcgataat agccggtagc ggcgctgcaa ttattgatgt ctcacgtgtt
    10321 gctcgtgatt acgcacttac tggccttgaa tttgcatgtg gtattccagg ttcaattggt
    10381 ggtgcagtgt atatgaatgc tggcgcttat ggtggcgaag ttaaagattg tatagactat
    10441 gcgctttgcg taaacgaaca aggctcgtta attaaactta caacaaaaga attagagtta
    10501 gattatcgta atagcattat tcaaaaagaa cacttagttg tattagaagc tgcatttact
    10561 ttagctcctg gtaaaatgac tgaaatacaa gctaaaatgg atgatttaac agaacgtaga
    10621 gaatctaaac aacctttaga gtatccttca tgtggtagtg tattccaaag accgcctggt
    10681 cattttgcag gtaaattgat acaagattct aatttgcaag gtcaccgtat tggcggcgtt
    10741 gaagtttcaa ccaaacacgc tggttttatg gtaaatgtag acaatggaac tgctacagat
    10801 tatgaaaacc ttattcatta tgtacaaaag accgtcaaag aaaaatttgg cattgaatta
    10861 aatcgtgaag ttcgcattat tggtgaacat ccaaaggaat cgtaagttaa ggagctttgt
    10921 ctatgcctaa agtttatggt tcattaatcg atactgaaac acgctgtcgc cattatttta
    10981 ccgaagaaga tattattgct attaaattta aatgttgtaa taaatactat ccatgctata
    11041 agtgccataa tgagtttgaa aagcacgcca ttaagcgttg gtctgagcct tcatttaacg
    11101 aaaaagcaat cttatgcggt gtatgcaaac acgaattaac aatcaatgaa tatatgatgg
    11161 tagagcgttg tccaaattgc caatctcggt ttaacaatcg ctgtaaatat cactatcata
    11221 tttattttga aatttaaaca acatagtcaa aacattatag ggagtgggtc agacatgata
    11281 aagcaccact tctgtagaat tcctttttga aattctctat gtttgggccc cgccaacttg
    11341 cattgtcagt agaaattgaa aatcaaattt ctctatgttt ggagcccact agtattgaaa
    11401 tgaacttgtt acaagtacat gttcattcaa ccaacgactg acaacagaac attttagagt
    11461 ctaggacatt gattgacgtc ccagatttcc ttttttaaag aaaagagggg agcaatgatt
    11521 aagaccttta accattgttc ccctctttta aaattatttt acaactttat caagttcctc
    11581 aatttgttta attgtagttg tagttgatcc agctgcaaag taccataatt taggatctaa
    11641 attatatact ttgtcttctt taattgcttt aacatttttt aatacaggat tatttaatgc
    11701 ttgtttcgca gttgatttac cacttatcgc ttgacctcta tccatcgcta aaataacatc
    11761 tggattttct ttattaacat attcgttaga aacattttgt ccatgattgc tattacttac
    11821 ttttttatca actgcattga atcctaatgt atcgtaaact aatccaccaa aacgaccttt
    11881 aggtccaaat gttgataatt caccttcgtt aactagtaaa tacataacag ttttattgaa
    11941 gtttttcgtt ttatctttca ttgaagcaat tttgttatct aaatctttat ttaattcttt
    12001 agctttatct tctttatcgt aaatttttcc gatattttca gtgttttgtt tcattgaacc
    12061 aattaagttc ttttcatctg caccaacata aacaattttc gctttaggtg cagctttttt
    12121 gaattcatct aaattctttt gattagctgt acgtccagag ataaagatta cttcgggttt
    12181 cgtcgcagca attttatcaa aattcacttc ttttaaatta ccaacgtttg tatatttatc
    12241 atctttaaat gattctaaga aattcggtaa tgacttaccg ccttcccctt taggtaatgc
    12301 ttttacttta tctgataagc ccatttcttt cattacatct aatgcgccat agtctaacac
    12361 aactgcattt ttaggatttt ttggtacttc aacagtttct ttaacttttt tagcgtcact
    12421 accatctttt ttctcgccac gcattttata gttattttca atttttactg tatctttaga
    12481 acctttagtt tctgattttg attgttcttt atcagaattg ttaccgcatg ccgctaataa
    12541 aaacattact gctaatacta aatataagac tgttttcttc ataatataat attctcctct
    12601 tttggcttaa tctgtgtgct tgatgtgaaa tataaataaa tgctttacgt ttaacccttc
    12661 tttaattggc gcatcacccc tttaaagtgt ctccaaatta tcaatcctta aaatataaac
    12721 aaatcctttg atttcttata tgttcaattt gaatattcat gtcatataaa tcacttaaaa
    12781 cactactttg aatgacatta tctttcaaat ctgacttaac tagttgtccg tttttcaatg
    12841 ctacaatctg atctgaataa caggacgcaa agttaatatc atgtaacaca atgacaatcg
    12901 ctttattcat tttatgcgct aacattttta acgtttgcat aatttgaaca gcatgcttca
    12961 tatctaaatt atttaatggt tcatctagca aaatatattc agtatcttgt gctattgtca
    13021 ttgcaatgta tgcacgctga cgttgtccac cagataatga cttaatatta cgatttctga
    13081 tatcttgtag ttgcaacaaa tctagcgcat cattgacaat atcatgatcc tctttcgtca
    13141 aacgaccttt agaataaggg aatcgtccaa agtttaccaa ctgctctacc gtaatattca
    13201 tttcagtatg gtttgtttgt tttaatatag atattttttt cgacaagtca ttatttttat
    13261 aatcagacat gagctgtcca tctattttca cttcaccgtt ctcaaaacga attaacctac
    13321 atatcgctga aagtaaagta ctcttacccg caccattagg tccaattaaa gaagtcaatt
    13381 tacctttttc gatatctatg ctaatatctt ccaatatcat ttgattattt atagttttag
    13441 ttaaattttc aacttgaatc attgcgcatt tctccttcta actaatagat aaatgaaata
    13501 acttccacca atcaaatcaa taagtatact catttctgtc gtagcttcaa acacattttc
    13561 aactacccat tgcgcactaa ataaactaat ccaactcaag caaattgtcg caattaaaat
    13621 atacttatgt tcatacgtct tcattagttc atgcgctaga tttacagtta ataaacctaa
    13681 aaatgtaatt ggtcctacta atgcagttga cacagaaact aaaactgaga ctattacaag
    13741 tagaattcgc gttaattttt catacgatat cccaagatta attgcttcag cacgacctaa
    13801 aagcaataca tctaaataag gcaatagtaa aattgtaatg actaataaga ttactaatag
    13861 cactgctgag aaagtaacta aattcgaatt agaagcatta aaattagcaa acatactact
    13921 ttgtattgct aaaaatgact caggatccat aatcagttga ataaaacctg ttatgcttct
    13981 aaaaaatgtt cctaaaagga caccaataag taagataaaa taaattgaaa aatgtccgat
    14041 tttaaagata ccttggaata ggattagtgc gaataacacc atcgttataa gtgttaatat
    14101 aaagttcaaa tatacattag taacccatac cgattgaatt ccaaataaaa agactggcaa
    14161 tactttgata aataaataaa ctgcatctaa ccccattatt gatggtgtca atagacggtt
    14221 atttgtaatc gcttgaaaaa tcaccactga agttgcaatg gcagcaccta ctaaaataat
    14281 taatatgaac tttcttaaac gacttgaaaa ttgatattca aatatttcaa aatcaatacc
    14341 tacaaataag tacagcatac taattaataa cgttactgca attaaaagca ttattttttt
    14401 attattgttg cgcataattt ttcctacctt tcataagcaa gataaggaaa atgattgttc
    14461 caaatacacc tattgttaaa ccaatattta tttcatatgg ataaacaaca attctgccaa
    14521 ctatatcaga aaataataca aagatggcac ctaacatcat cgtatgaggg atagcatttt
    14581 tcaaatgatc acctcgataa attgaaataa tatttggtat tactagtcct aagaacggta
    14641 atgttccaac agtcaccact accaatgctg taatagttgc agtaataaac aatgcgatgt
    14701 taattaattt ttcgtaactc aaacctaaat tattagtaaa gtctttaccc attcctacaa
    14761 tcgtgaaatg attagcaaaa agatatgtca atgctaaaag aggaatactt aaatataaaa
    14821 tttcatagcg tccacttgtg ataatggcaa agttcccgtt aagccagtta ccaatgcttt
    14881 gaacagcatt cgtgcgcaat gagatgaagg ttgtgaaact tgcaacaatc ccacccatca
    14941 taattcctaa aagcggtaca aaaatgacat ctttcacttt tataaaatca atgattttaa
    15001 caaataaaaa cgtaccgcaa atactgcaaa taacagcaaa tactagtttt aataaaatat
    15061 gaccggttgg aaagaacaat aaagcaatta aaatacctag tttagcccat tccatcgttc
    15121 cagctgtagt cggactaaca aacttatttt gcatcatttg ttgcattatc aagcctgcta
    15181 aagccaacga acttccagca attaaaatac tcatcgtcct tggtattcgg cttgagaata
    15241 aaatattccg ctgtgactca gttaaatgta gtaaatcttt aattgatagt tcactcacac
    15301 ctatgaacaa cgaaataatt gtcaacaata ttagtaatag aagcaaaata tttcccttta
    15361 ataaatactt cattacaaat ttttctccct ttaaattaat gataatcatt atcattgata
    15421 atatttctca ttaatagtag cgttttttga ccataatttc aatagccttt caaataattt
    15481 agtgtcaatt ttattgatat ttgcaattaa actgaaattt aatttttcgg aatgtgtatt
    15541 ttgtttactt aaagtaaaat agaacactta agattttgat gtctgggaat agtggaaatg
    15601 ataaaaacta ctaatgattg attaagtagt ggttcttaac attagcctca gctaatgtgt
    15661 acttaaaaat aggaatacat gagtaaaact catgtggaag tgataatttc taaaggaaaa
    15721 agtttttctt tatgttgggg ccccgcccga acttgcacaa tattgttagc tgactttccg
    15781 cctgctttta tgttggggcc ccgccaactt gcacaatatt gttagctgac tttccgcctg
    15841 cttctatgtt ggggccccgc caacttgcac aatattgtta gctgactttc cgcctgcttc
    15901 tgtgttgggg ccccgccaac tactgccaat ataaatagca ttgtacatct tagagcattg
    15961 atttcttact cagactcata atgagttaga tacacataaa aaaagacctt caacatgtag
    16021 gagacaagac gaaacgaatc gctatttccc tttacatgtg aaggtctttt aatttaattg
    16081 ttatttgttg tcaaatacaa agtcatcatc ttgtaatgct tctacgttta atgctaatac
    16141 ataaccatca ccttttactg agaagaagtc atggttttta gttgttgtat ctaaggcatt
    16201 ctcaatgatt gggttaaatt cacgttcctc aaaataaggt tcaaagccta agtttgaaag
    16261 tgctttgttt ccattatatt taacatagtt tagcacatct tcagtgattc caagatcatc
    16321 gtataacatt tttgtgtatg actcttcatt taaatacaag tcatttagca atttatacat
    16381 ttcttgatct gctttttgtt tctcactttc agatagttca tttcgtaaat gctgtgcatc
    16441 taaaccggta aatacaccat gaatagattc atctaaaaga attttacgaa tgatttcacc
    16501 tgatgtcgtc attttccctt gaccagcaag atatagtgga taatagaaac ctgagaagaa
    16561 taagaatgtt tctaaaaata cactcgtaac tctggccata tattggtcgt atatcgaagc
    16621 ttctttaccc caaagtttgt gataattagc aacaatttta tcagatttat attttaaatg
    16681 gggttcctct aaaacccatt catctaatag gtagtttgtt tcactagatg gtaatagtgt
    16741 tgtgaaaata tgtgaatagc ttttcgcgtg tatttgctcc atcatcgcca taaatgaata
    16801 aactgctttt ttccttaagt cagtcgtatg tagcataact aaaggcatgc catcatctgc
    16861 ttgatgtgta tctaagcctg ttaaaccagc taatgctttt ttaaatgtgt cttgttcagc
    16921 ttcagataaa gtcttccaac ttgcaatgtc ttttgatact ttaaattctg tttcaaccca
    16981 catttgagat atattttgtc tccaaaacat attcgtcata tcttcttgtg tgttccaatt
    17041 aacagctatc atgaatataa tttcctcctg taatgttact caataattga caaatacaga
    17101 catagcagtc taatcatctg ctaattcaga gcagatgatt agctgtttgt cattttcaac
    17161 atttaattgt tagatagaac
//
LOCUS       DAKTIP010000037        16966 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAKTIP010000037 DAKTIP010000000
VERSION     DAKTIP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16966
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.037"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            80..406
                     /locus_tag="O0305_000966"
     CDS             80..406
                     /locus_tag="O0305_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCY0274119.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            436..1455
                     /locus_tag="O0305_000967"
     CDS             436..1455
                     /locus_tag="O0305_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCY0274120.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(1691..2344)
                     /locus_tag="O0305_000968"
     CDS             complement(1691..2344)
                     /locus_tag="O0305_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCY0274121.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPTLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGEALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDMLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            2518..3204
                     /locus_tag="O0305_000969"
     CDS             2518..3204
                     /locus_tag="O0305_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCY0274122.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(3270..4529)
                     /gene="sdpC"
                     /locus_tag="O0305_000970"
     CDS             complement(3270..4529)
                     /gene="sdpC"
                     /locus_tag="O0305_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCY0274123.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKDAEKTGVATASTVGVAKNDTENTVADEPSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            4819..5754
                     /locus_tag="O0305_000971"
     CDS             4819..5754
                     /locus_tag="O0305_000971"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCY0274124.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(6010..6429)
                     /locus_tag="O0305_000972"
     CDS             complement(6010..6429)
                     /locus_tag="O0305_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0274125.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
     gene            complement(6551..7435)
                     /locus_tag="O0305_000973"
     CDS             complement(6551..7435)
                     /locus_tag="O0305_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0274126.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            7595..9256
                     /gene="hutU"
                     /locus_tag="O0305_000974"
     CDS             7595..9256
                     /gene="hutU"
                     /locus_tag="O0305_000974"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCY0274127.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            9256..10494
                     /locus_tag="O0305_000975"
     CDS             9256..10494
                     /locus_tag="O0305_000975"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCY0274128.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            10771..11892
                     /locus_tag="O0305_000976"
     CDS             10771..11892
                     /locus_tag="O0305_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCY0274129.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            12003..12884
                     /locus_tag="O0305_000977"
     CDS             12003..12884
                     /locus_tag="O0305_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0274130.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            13371..13940
                     /locus_tag="O0305_000978"
     CDS             13371..13940
                     /locus_tag="O0305_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274131.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      13993..14088
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            14165..15478
                     /locus_tag="O0305_000979"
     CDS             14165..15478
                     /locus_tag="O0305_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCY0274132.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     gene            15645..16151
                     /locus_tag="O0305_000980"
     CDS             15645..16151
                     /locus_tag="O0305_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCY0274133.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(16209..>16966)
                     /locus_tag="O0305_000981"
     CDS             complement(16209..>16966)
                     /locus_tag="O0305_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0274134.1"
                     /translation="DEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFTHAS
                     NATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERVDYQ
                     LLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKSEPN
                     DLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTFYLT
                     DGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
BASE COUNT         5683 a         2375 c         3378 g         5530 t
ORIGIN      
        1 atgtgaagta agaagaaatg taaacaaatg atatgtagcc gatagattat tgatagcaaa
       61 tgtgtgatgg agtgatttca tgttatatcc gatttttata tttatattag ctggcttatg
      121 tgaaataggt gggggatacc tgatttggct gtggcttagg gaaggacagt cttcacttgt
      181 tgggctaata ggcggtgcga tactcatgtt atatggtgtc attgcgacat ttcaatcatt
      241 tccatcattc ggaagagtat atgcagcata tggtggcgta tttatcatca tgagtttgat
      301 ttttgcgatg gtcgtagata aacaaatgcc agataaatat gatgttatcg gtgcaatcat
      361 ttgtattgta ggtgttttag taatgttgct acccagcaga gcttgaacac ttgtggttag
      421 aagggagaag taaaaatgat agttgaagtt gagcaccaaa gacaaggtgt agatttaatc
      481 aaaattgata atgatgaaac taaaattatt tttacgaatt atggagcaag agtagtgtct
      541 tggaaatatc atgataataa tattgttctt ggaaatgttg ttgaagcgga tgaattttat
      601 catagtaatc cttttaaatt tggtgcttct ataggaagat atagcggtag aattgataat
      661 gctaaattta agttgaaagg taaagaatat cagttagaaa aaaacaacgg tgaacatcat
      721 ttacatggtg gatgtcatgg tttggacaat aagttatttg attatgaaat tagaaatgaa
      781 attgctcaaa ttaaagttat atttaagacg gtgttgaaat cagctgatga tcatttcccg
      841 ggtgatatcg atgtgacgat tacacatatt tatgatgcgg atcaccaatg gtcgattgaa
      901 tacgaagcag ttgcatcaga agacacattg tttagtccta cgaatcatgt gtacttcaat
      961 ttgaatcgag ataataatgt cgttgataat catcgcattt caagtaatca attagatatg
     1021 tatgtattag atgagcgaaa tattgttaca ggtgatattc tagatttaca tgaagtgttt
     1081 gaagataaca agataaaatt gagtgatatt tttacatcac aacatgcgca attaagccaa
     1141 caaatgacac gttttggtgg tctcgatcac ccatttacag ttggtgaaca taaaatgtat
     1201 gttgaaaatc atgaatttat gttagaggtc gatacagata tgcctcatgt tgtgttcttt
     1261 acttttaacc agcctgatga gtgggatagt ccatttaata tttataaacc acattctggc
     1321 tttacattag aaacacaatt tttaccaaat gatattaatt tatatggtga aaaagcaccg
     1381 tcaattttaa aagctggtga agtgttcaat tcgaaaacaa gtttccgtat cgaagaaaaa
     1441 gtagaaaatg attaaaagta tcggcattta tataatattt atgttatttg gcggaatttt
     1501 gaataggtta tcaagaatcg tatcaaatga agtgacatca ttaatataca gtttgaaata
     1561 aagaaaatat ataacataaa aaaggaatca atagcatcag tggttataga taagactatt
     1621 atacaaacga tgtgtaagtt gattcctttt tgttttgaaa agttcattta acacttataa
     1681 tgaaaggtaa ttatttcaat gttctgttat acattttttg aagtttatcg cgtcttgttg
     1741 gtgacagaaa tggattgttt agtgcatatc gtaacatatc ttgattttgt ctatcattat
     1801 aataaaggtc attaagctgt tgtacagata aacgatgttc gattggtgct tcttgtatta
     1861 agtaaagctg atcatttgga gatacatagc cttgttttaa tacccggaaa tagaaacctg
     1921 ttttaccaga tgtagacatg cgcttcacta aatcaggaat attatatttt gcttgaattt
     1981 tccagcatgg ttctctaatt tcagaaacct caattaacgc ttcacctaaa cgatattgat
     2041 taccaaagta aacatcagat tcgtctaaat aatcaaatgt taaattctct ccaaacatcg
     2101 catgagtagg tagtgtaggt aaatcatctt gatacagtgc ataattatgc ttactaaacc
     2161 cacaaactgc tttatgtggt ccaccatgat ctttataagc ctgttcatca ccagtgaaac
     2221 cagttgaaga taaccatgtt gtttgtgaaa tcttagtttt atctaaagca gagcgcatcg
     2281 gtcttttcga gctataaggc aaatcttgga tttttcctgt cgaaattgcg tgaattgata
     2341 tcatatcatt ctccttaacg agtattattt aaaatatatt gtactataaa acatttttag
     2401 aattgtaaat tctgattgat atgtttaaaa aaatgttaga aataagggta tgttaattta
     2461 tacgagtcat gtaaaatatg aattgaatag attacataaa tatgggaggc taccttcatg
     2521 aaagatgtca aagcactcaa gttaatgaca ttaaatgatg ttttaagtca aatcaatggt
     2581 gacatgacgc tgggaattgg tacaggaagt acaatggaat tactcttacc tcaaatggcg
     2641 caactaatta aagaacgtgg ttacaatatt acaggtgtat gcacttctaa taaaattgca
     2701 tttttagcaa aagaattagg tattaaaata tgtgaaatca atgatgttga tcatatcgac
     2761 ttagcaattg atggtgctga tgaagtagat ccatctttaa atataattaa aggtggcggt
     2821 ggtgcgctgt tcagagagaa agttatagat gaaatggcat cgcgttttgt tgtggttgtc
     2881 gatgaaacga aaatagttca atacttaggt gagacgttta agttaccagt agaggtggat
     2941 aaatttaact ggtatcacat attacgtaaa attgaatcat acgctgatat aaaagtagaa
     3001 cgtcgtgtaa acgaagatgt cgcatttata actgataatg gcaattatat tttagattgt
     3061 aagttaccaa aaggaattga cccatataag ttccatgagt atttaattca tctgacaggt
     3121 gtgtttgaga caggatactt cttagatatg gcagatcaag ttattgtagg cactcaagag
     3181 ggtgttaaaa ttttagaaaa ataatatata gtatagaaaa gcgcttgcta gcatcatctt
     3241 tttaagaggt actagcaagc gctttgttat tatttgtttt tatctgaaga ttgttcttca
     3301 gttttttcga tatgtttttc tgcatcaatt acaactgatg aacgtctgtc ttctttataa
     3361 gtcgcaggtt cttttaatga ttgctctatt tcatcttcat ttgaatcgtt tgttaaatcg
     3421 tcactttgtg gtgcttgttt aactgattct gctgattcta ctgactccga agtgatgtca
     3481 tgatcttcat catgattaga ttcagtttga ttacctatgt gatgttgagg ttctgttttt
     3541 tcagtacctt catgaatgct tggttcgtca gccactgtat tttcagtatc atttttagca
     3601 acaccaaccg ttgatgcagt agctacacct gttttttcag catctttaat atttgaagaa
     3661 gatttttcag cctcagtatg attagtttct tcatcgtcat tgacatggtc taaataatta
     3721 ttaggctcaa gttcttcaag gtttcgtctt gttgttaaat atgcaatacc tcggataatt
     3781 aagcttaaac caatgtatcc tactgcaacg attgctgttg aaatggcgat gactttgatt
     3841 gaaaatagat cgccgatttc ttcgctaaac aagaaaataa gtccgaatgt cattgaagca
     3901 tggaatgtcg ttgcaatata aattgtacgt cctttagtcg ctctaattaa ttcaccaaga
     3961 atcatagaga atgagaatgt ataaaggaag ttataagcag caaattctgt accataagtt
     4021 gtatttgctg agaaaactga atacatcaaa ccaacaacaa tacttgcaaa gaacgtgttc
     4081 attttcgttt caacaatatt ttgtaagtat gaacggaatc cgaattctac tacgaacgcc
     4141 atcagaatat gtccaatcag aatgtgtgta ataggtactg ataagcctgt tgattgtaat
     4201 aaaataaagc tatctgcaaa tgtattaaag ctgtacatac caataattag aataattaaa
     4261 ggtaaaatta aagctagcaa tagacgttca attactttca ggctgattga gaattttaaa
     4321 cctgcaagtt ggaccttttt atatttgaaa acgagtatac aaatgattgc agcaattaat
     4381 ggtgctagat ctgtaacttc aaatataaag tgtttgacac caattataga ctggaaatct
     4441 ctgagcataa tggataacgc cattgtaatg acaaagaaga cgaaaatcgt cattgcccat
     4501 tgaaaaccag aaattttatt gttcttcatt atatgtaacc tccattaggt aacaagcaaa
     4561 atatctttta gtaacaatta tacatattac aagccgagag ataaattgtc tatgtcaata
     4621 ttaatgaaaa tgtaatagtc ttgacgtatt ttattaacat aaatcagcta agtattcgtt
     4681 attacataga aaataggggt aaaagtaatg tagataagta tttttaagat acggatttaa
     4741 ctatgaattg aattcaaaat ttcctatacg ttatacttta attgttaata agccacataa
     4801 ataagagggg gaaatgctgt gtacaagcaa ggtgaaccaa atttatggac tggaaggtta
     4861 gatagtgaaa cagacccgaa aaaatttaga cattttcaaa cagtaacatt tgaagatttg
     4921 tctaagctgg aaaagagtag tatgccatca ggggtcggta tattaggcta tgctgttgac
     4981 aaaggtgttg ctttaaacaa ggggcgcatt ggtgcaaaag aaggaccaga tgcgattaaa
     5041 caagcatttg caggtttgcc ggatttgaat caatgtgaaa ctttagtcga ttacggaaat
     5101 gtttatcatg atcatgagga attaattgat actcaaaaag aatttgctat gcttgcagcg
     5161 aagtcaattg ctaatcatag acaaacattt ttattaggtg gtggacatga tattgcgtat
     5221 gctcaatatt tagcaacacg taaagtctat ccaacacaat ctattggtgt gataaatatt
     5281 gatgcgcatt ttgatacacg tgctgaacaa caatctacat ctggaacgag ctttagacaa
     5341 attttagaag aagatgaaaa cacagattat ttagtgcttg gtattgctca aggtggtaat
     5401 acgcaaagtt tatttgatta tgctaaagag aaaaagattg attatgtctt tgcagatgaa
     5461 ttattgagtc acgtatcacc aacaattaaa gatatgattg aacgttttgt acatgaacat
     5521 gatgtcatta tgtttacgat ttgtatggat gtcattgata gtgcgtttgc gcctggggta
     5581 agtgcgccgg cagtgttagg tttgtatcca catactgttc ttgaattagc aaaacgtatt
     5641 attccaagtg ataaggtgtc ttcagttagt attgctgaga tgaatccaac atatgacgct
     5701 gacaatagaa ctgctaagct cgttgctaat ttagtgcatc attttttaaa ataattaaaa
     5761 atatttatat gtgacgagat ttgcattgca gggcagaatg tgagtatttc ttaatagaag
     5821 ttatattaac gttgatatat ttgaaaaata attgcagtaa gaagactctt aagttgtagt
     5881 catcttactg cttatttatg tttgttgaaa tagcaaaaat aacaactgta ttgtttgaaa
     5941 agttctctta ttatataata attaattaaa attttaacgg agttcaagag gcctttttat
     6001 aatgacacct tatttgtaaa atgtcatatg tggtttagcc tctttataat aatttaatct
     6061 gttctcaagt gtgccagtat gtaattctag cttatgacca tcagggtcgg taaagtaaat
     6121 tgattgtcta tctctaatat ctctaactct tccttctaaa atattcacgt tattatcttt
     6181 taacctctga tgccaatatt taaattcgct gtcatctata gtgaaagcta tatgtgtata
     6241 tgaaaagtga atttcattac gtggtatatc tttttcttca tttaaagcaa tccataggcc
     6301 tgcaagctca aaataagcag tttttttacc agtcaatagc aatttcccaa gtaaaatatc
     6361 tctataaaaa tgtattgaat cgtttaaatt tctgactgaa aagcatatat gattaataga
     6421 ttttaacatc tgcttcactt ctttcttaga atttttataa taaattattt ttgtatcgat
     6481 ttttgaaatg acgtcattaa tttttatacg atgatctgcg caactgcata agaggctcta
     6541 ataacttaag ttaaagagcc tctaaaagtc catataaata atcttggcat tgttgtacga
     6601 atgtatgtac ttgttcggag tgtttgcgtt ttttatggta attgatataa atttttcggc
     6661 ctaagtttgg acgaattttt ttatattcta aattagacgt gtgaaatgat tggtaataaa
     6721 atctcggaat gatagcgtaa cctaagccga gatgaacaaa tccaactgct gattcgaatc
     6781 gatctgtttc tacaacgaca tttggacgaa tatttcttct attaaaatag tcatctaagt
     6841 gtttgcgcac ttgagaattt ttgtttggta atatgagtgg caaattttca acatctaccc
     6901 aattttgatt tttaaatgtt tccttgggtg ctaataaaat gtaagattcc tcatataaag
     6961 gaatggatct tatatcttcg tgggttattt tttcatttgt tatagctaaa tgaatattaa
     7021 aattcagtaa ttgctctata gattgatgtt tatcatgtat ttcatataaa cgatattgct
     7081 gttcagggta gtcggaatgg tgctttcgaa ttaaattcgc aatccattga ttcgtagatt
     7141 caagagtccc aatttttatc cttggttctg atgtaacgct taaatcatac attttttcca
     7201 tcgtggatcg atattgttga actaattcgc tcgcataacg ataaaactgt attccttttt
     7261 cggtaatctt gatgtctttt gttgaacgtg taaataagtc ataacctaaa tctgcttcca
     7321 tttttttaat cgtagcagtt aaagatggct ggctaatatg taaaaattgt gcagctttag
     7381 taaaactatt atatttcacg atagccaaga agtattctaa ctgaataatt ttcatgtgtt
     7441 ttgctccttt tatttataga ttcaagctat ggataattag tttatttata tttgttggct
     7501 atattttaac atactataat gaatattgaa gataataatg taagcgtttt aaatttagtg
     7561 gaattaactt cttaaaagaa caggagcgaa ttttatgaga aaaattcaag caaaaaaagg
     7621 tttgagtatt gagtgtaaag gttgggaaca agaagcagta cttagaatgt tatacaacaa
     7681 tttagaccca gaagttgctg aaagaccaga agatttagtt gtttatggtg gtattggaaa
     7741 agcagcacgt aattgggaag cttttgaagc aatagagaaa acgttacgag aattagaatc
     7801 agatgagaca atgttagtac aatcaggaaa gccagttgct gtatttaaaa cacatgaaga
     7861 agcgccacgt gtattaattt caaattcagt attagtgcct gaatgggcaa actgggatca
     7921 ttttaatgaa ttagataaaa aaggtttaat catgtatgga cagatgacag ccggtagttg
     7981 gatatatatt ggttctcaag gtattgtgca aggtacttat gagacatttg ccgagctagg
     8041 taatcaacac tttaatggag atttagcagg cactgttaca ctgacagcag gtttaggtgg
     8101 tatgggtggt gcgcaaccgt tagcaattac tatgaatcat ggggtagcaa tttgcgtgga
     8161 tgttgatgaa acacgtgttg ataagcgaat tgatacgaaa tactgtgatg ttaaaacagc
     8221 tgatttagat gaagcattaa aattagcaga agaggcgaaa gaacgtggag aaggattatc
     8281 aatcgggtta gttggaaatg ctgtagacat ccatcaagcg attctagaaa aaggatttaa
     8341 aattgacatt attactgacc aaacaagtgc acatgatccg ctaaatggat atgtgccaca
     8401 aggatactct gtagaagaag cgaaagtatt gcgtgaaaaa gatccgaaaa aatatgttga
     8461 actgtcacaa gcttcaatgg caaagcatgt tgaattaatg cttgaattcc aaaaacgtgg
     8521 cgctgtagca tttgattatg gtaacaatat tcgtcaagta gccttcaata acggagtgaa
     8581 aaatgctttt gacttcccag gctttgtacc agcttacatt agaccattat tctgtgaagg
     8641 taaagggcca ttccgctttg ctgcgttgag tggtgatcca aaagatatcg agcgtgcgga
     8701 tgaagaaatg cgcaaacttt tcccagaaaa tgaaaagtta ttaagatggc ttgatttagc
     8761 tgaagaaaaa atttcatatc aaggactacc atcacgtatt gcttggttag gctatggaga
     8821 aagagcgaaa atgggcttag ctttaaatcg tcttgtacgt gacggtgaaa tttcagcacc
     8881 aattgttatt ggacgagacc atttagatgc tggttcagtt gctagtccta accgtgaaac
     8941 agaaagtatg aaagatggta gtgacgcggt tggtgattgg gctgtattga atgcactcat
     9001 taacacagct gcaggtggtt catggatttc attccatcac ggtggcggtg ttggcatggg
     9061 atattcactt catgcgggta tggttgttgt agcagatgga tcagagcgtg ctgaaagaag
     9121 attggaacgt gtattgacga ctgacccagg tatgggtgtt gcccgacatg ttgatgctgg
     9181 ctatgacatc gctattcaaa cagctaaaga aaaaggtatt catattccaa tgattgataa
     9241 agcaggtgat aagtaatgaa tgatttaata attaatcata tagcagaatt aattttaccg
     9301 agatcaacag ataaaccttt gaagggtaag gaattagatg aattaaatgt tgtgaaaaat
     9361 ggtacagttg tcattaaaga tggcaaaatt gtatatgctg gaacacatac agatgactac
     9421 gatgcgactg agacgattga tgctagtggg aaggtagtgt ccccagcatt agtagatgca
     9481 catacccatt taacatttgg tggatctcga gaacatgaga tgtcattaaa acgtcaaggt
     9541 aagtcttacc ttgaaatatt agaaatgggt ggtggcatat tatctaccgt taatgctact
     9601 agagaaacat ccgaagatga cttgtttaaa aaagcagagc atgatttgct tactatgatt
     9661 aaacacggcg tgcttgcagt tgaaagtaag agtggttatg gattagatag agaaaatgaa
     9721 ctgaagcaat tgaaagtgtc taatcgttta gctgagaaat atgatttaga catgaaacat
     9781 actttcctag ggcctcatgc tgtacctaaa gaggcaagtt caaatgaggc atttttagaa
     9841 gaaatgattg cgttacttcc ggaagtaaaa caatatgcag actttgcgga tattttctgt
     9901 gaaacaggtg tatttacaat agaacaatcg caacattata tgcaaaaagc caaagaagca
     9961 ggttttaaag tgaaaataca tgcggatgaa attgatccgt taggcggact ggaattagca
    10021 attgatgaac aagcaatatc agcagaccac ttagtagctt ctagtgataa aggaaaagaa
    10081 aagttgcgaa atagtgatac tgtagctgtc ctcttacctg caacgacgtt ctatttaggt
    10141 aaagaagatt atgcagatgc aagaggcatg cttgacaata acggtgcgat tgcattagca
    10201 actgattata accctggtag tagtgtcaca aacaacttac aacttgttat ggcgattgca
    10261 gccttgaaat taaagctatc acctaatgaa gtttggaatg ctgttacggt caatgctgct
    10321 aaagcaatag acattaacgc gggtacaatt aacacaggtg ataaggctaa tttagtaatt
    10381 tgggatgcac caaatcatga atatattcca tatcattttg gtatcaatca cgcagaaaaa
    10441 gttattaaag acggtaaggt catcgtagat aacacagtat cttttaaagc ataaaataat
    10501 tagaaagctc taagcattgg tttaaactaa tgtttagagc tttttgtggt ttgtaaaaac
    10561 aagtatattc gagaacacgt ttttgtatag agtctcaatt ataaaagtta gaatagtttg
    10621 acaacagtgt tacgatatta ttaaacaact gtctatcatt aacgaaagca attaacaaga
    10681 gtacattgtt gtgaaatcat cgtttaatag tcaaagatga tcatcgtagg gtagacaaca
    10741 tatcattgac ttaaggaagg ggaatgaccg atgggattaa aagatcaagc tgttacctgg
    10801 agaaggtatt ttcatcaatt tccagaacta tccgataaag aatttaagac tacacaaaaa
    10861 attaaagata ttttaacaga acatcatatt agaatattag acttaccact tgcgactggg
    10921 cttgttgctg aagtgggaca aggtctgagt tgtatagctg ttcgggctga cattgatgct
    10981 ttaccgattc aagagcttgt tgaacaggat tttaaatctg aaaatgaagg tgttatgcat
    11041 gcgtgtggtc atgatattca catggctagt attttggcta cagctgtaaa attaaaagaa
    11101 attgagggca ctcttactgg gcgtgttaaa ttcatttttc aatcagcaga agaactagga
    11161 catggcgctt ttaaaattat tgaaacacat gcattaaaag atgtacaagc agtattgggt
    11221 tttcataatg acccatcgcg ttcggtaggt acatttgcaa tcaaaacagg ggcaattaca
    11281 tcagcagtag atcgttttga gtttcatatt aaaggcgtgg gtggtcatgc tgcaaagcca
    11341 gaacaatgca acgatccagt tattgtgttg gcgcaattga ttaatagtat tcaatccata
    11401 gttagtagaa atctatctgc gtttgatgaa gcggtagtaa caattggaca aatatcatgt
    11461 ggtaatactt ggaatgtcat tgctgatcat gcgtatgtac aaggcacagt gcgtagtttt
    11521 gatcctgtgg tacgaaagct tgtagaaaca cgattacaag atattgcgga cggattggct
    11581 caagcatata atatgaaaat caatttaaat tatacgcatt tacctggcgc tgtgatgaat
    11641 gatgaggcat tgacgcataa agcaattgca gtagcccaac atgttggata taaggtggaa
    11701 atgatggaac agccgcttac gattggagaa gatttttcag gttatagtca acattttcca
    11761 agtgtatttg cgttaattgg ttctcatagt gaatatgatt tacatcatcc tcaatataaa
    11821 ccggatgaaa ggattctgga aaaagtacct gagtattttg ttgaatttgt aaagagacta
    11881 ttgcatgaat aattcgagct tgtttttata aagaagaaag taatttttag tgtaatcatg
    11941 attaagttcc taggaagatt gggtaaataa taaccattaa gcgaataagg agatgtaaaa
    12001 aaatggcagc tcaagatcct agaacaaaat ttaaaacaac ggattatgaa aaacaagaac
    12061 aagaggtacc gggtttacaa tctgaaatga cgccagcacc cgattgtggc gaaacgtctt
    12121 accaaggtca tcaacgctta caaggctata aaatattagt cacaggtggt gactcagcaa
    12181 tagggcgtgc agcagcaatt gcgtatgcta aagaaggtgc agatgtagcg attaactatc
    12241 ttccgagtga agaacaagat gcacaagaag tacgccaagt gattgaagaa agtggtcaaa
    12301 aggcagtgtt aattcctggt gatataagag atgaacagtt caactatgac cttgttgaac
    12361 aagcatatca acaattaggt ggcttagata atgttacttt agttgctgga catcaacagt
    12421 atcatgatga tattcatgga tttacaactg aggcatttac agaaacattt gaaacgaatg
    12481 tctatccgct attttggaca gttcaaaaag cattggaata tttaaaaccg ggtgcgtcaa
    12541 ttacaacgac atcatctgta caaggttata atcctagtcc tattcttcat gattatgccg
    12601 cttcaaaagc agcaattatt tcattaacta aaagtttttc agaagaatta ggaccaaaag
    12661 gaattagagt gaactgtgta gcgcctggtc cattctggtc accattacaa atttctggtg
    12721 gacaacctca aagtaaaatc ccaacatttg gtcaaaaaac acctttaggt cgtgcaggtc
    12781 aacctgttga gttatgtgga acttatgtgc tattagcgtc agaagaatca agttatacaa
    12841 ccggacaagt gttcggtgtt agcggtggag tgcaaataga ttaggtttgt atgaagcttt
    12901 taattgaaca acataatatt aatttagtta cttcaatcca ttaaattaaa atgaattggt
    12961 gaagtgtatt ttaaatagtc ggtaaaggtg tgtatcttta tcggcttatt ttaatactct
    13021 gtcataagga gcgtttgctc agggtgtgga ttataagttt taagcagaat aaactttctt
    13081 aatgaggatg tcaggacaac aacgaaaaag ttaaaaactg ttgcgggatt gggtttaggg
    13141 acggttagtg gaagaaagtt aaattcatta acaagtgaaa tgcctaaggc tagatattaa
    13201 acgtcagaac ggacaaagaa taataggata tgttttcaat taaatagcga agtatttata
    13261 tgttaagatg atgtaaatta catagaaacg ctacccgtag aagtactgat catagaaacg
    13321 ctgataaacg taaatttgca aactcgatat taaacaaagg ggttgttatt atggcattaa
    13381 tagtatctag agctatatta tttttgattt tagcattagc ttttggaata gcgactgttt
    13441 ttcctgaaca aaaattacca ttatatatag tattcacatt atttgtagca gttgcgatcg
    13501 tttcgaatgt atggtttaca catcaaaacg ttaaaaagca tatcattaag tttaacaaat
    13561 acgtcgcata ctttttatcc tttgtctcag ggttacttta cattggattt gtaatttata
    13621 cattaactat agggagctat gatgttttac caattattat atttgcaata agtatcgtat
    13681 tggttcaaat attagaaaat atactgggaa tcagtataaa aaatacgcgt ctatcaaaca
    13741 aacagttgaa agtcatctcc aagcaacgtg ttcagcaatt taaagtcata tcattaatat
    13801 tagtaagtgt gtctatcgtg ttcattttta ttgcattaat gcttaaatgg tggcttttac
    13861 ttgttattag cattattgtt ttgattatta ttacagtagc aatgattgtt tatgaaaaat
    13921 attatacgac tcctgattaa tttatgattg acgtactaag gattatcttg taacgtaaac
    13981 gtaaataagc atcacttatc tagagaggtg gagggactgg ccctatgaag cctcggcaac
    14041 atctcgaatg tgccaattcc agtaaccgta atggtttgaa gataagcagg taaagcacat
    14101 gaaaacctct ttcttcatcg tttgtgagaa agaggtattt ttaattggaa agcaggtaaa
    14161 aaggatggaa gtacataaaa agagcaatgc ttgggcatta ttccccttgt tattatttgt
    14221 ggcgttgttt ttaggcgtag gtattatcac aggtgatttt acttcaatgc cattaaatgt
    14281 tgcaattacg ataacggtaa ttgtggcgtt attaatgaat cgaaaagaat catttgctaa
    14341 aaaagttgag gtcttcacga aaggtgcagg ccattcaaac attgttttaa tgatgttgat
    14401 ttttatttta gcaggtgcat tttcaaatac agctgaaaaa atgggtggcg taaagtcgac
    14461 agttaattta ggactatcat taatcccaga aaatttaatt attgtaggat tgtttgtgat
    14521 ttgtatgttt gtttctatat ctatggggac gtctgtagga actgttgctg caatagcacc
    14581 tgttggttac ggatttgcac aagcgacaga tgtaccaact gcgctagcaa tggctacagt
    14641 tgttggaggt gcaatgttcg gcgataatct atcaatgatt tcagatacaa caattgctgc
    14701 ggttagaaca caacatacga aaatgaaaga taaattcaga gtaaacttta aaattgtatt
    14761 gccaggtgct attttaacga ttattattct gtatttctta acgaatggca tttctttaaa
    14821 tcatgctaaa aactatgatt ataatctaat taaagttgta ccgtatatct tagtattggt
    14881 attagcatta ttaggtgtaa atgtcattat cgtattgatt ggcggaacat tattagccgg
    14941 aatcattggt ctcatagatg gttcattcgg ttggatgggg ctactagatg cagtgtccaa
    15001 aggtattata agcatggaag atattgctat gattgcgttg ctaattggtg gtttagtcgg
    15061 tattattcaa cacaatggtg gtattgaatg gttgttgcaa tttgttcgct ctaaagtgaa
    15121 atcaaagcgt ggcgcagaat taggaattgc tagtttggta agtgtcgcag atattgccac
    15181 ggcaaacaac accatctcta ttattatgtc aggtccttta gctaaaaata ttgcagatga
    15241 atatgatgta gattcaagga aatcagcaag tattcttgat atatttggag gctgcttcca
    15301 aggtttatta ccatatagcc cgcaagtcat ttcagcggct ggtgtagctg gtatttcacc
    15361 gttaatgtta ttcccataca gcatttatcc tatattatta ggtgtatgcg gacttattgc
    15421 gattttattt aattttccaa agctaaaaaa gaacactagt caagaaatga aacattaatg
    15481 cttaacaata tatcgatgta gccaagtaag gtggcttgtt atattaacag atcatgttgt
    15541 actcaccgta taacgctaat cttttaaaaa tgatgatcat ctatgtttgg aactgagcaa
    15601 aaatacgtta aaataaaagt aagagcgaaa gtgggtggac aacagtggca aaatttaatg
    15661 tagagaatga acatgtcgaa gttgaaattg aaaagcttta taaattttct ccagagttag
    15721 tatacgaagc ttggacaaag aaagatttgt tgaaacaatg gtttatgaca tcagccagaa
    15781 cgaataaaga gattgaagcg gacgttaaag aaggcggtaa atatcgtatt gtagatcaac
    15841 aacgtaatgg caaagtaaat gtgattgaag gcatttatga atctttagtt atggatgaat
    15901 acgttaagat gactatcggt atgcctggac taagtgaaac gcaagatgtc attgaggtag
    15961 aattttttga acgtgaaaca ggtggcaccc aaatgctatt ttattatcgt tcattagttg
    16021 aaaaagaaag acgatttact aatttagaat acaagcaaaa gaaaaaagaa tatcatgatg
    16081 ccatggtgca tggtttcgaa ttgatgtttg ataagatgta ccacgttatc gaaacatcaa
    16141 cacaacaata aaatgataaa aggaagttat gataggctca aggccattaa atcataactt
    16201 ccttttttct aatttgaaat atcattatgt agtaaatact cattatattt tcttacaagt
    16261 gtttcaaaga atatatgata actgatgaag ctagaatagt ctgtaccatc agttaagtag
    16321 aatgtgttac tggatacata gacgttgtaa tttgcttttt gagcaagggt attatctttc
    16381 attgtagtaa cagaaataaa gtatttttgt ctaagttgaa gcaagtttgt tacttctaat
    16441 aattcatgtg tttcacctga taatgaaata ataaagaata aatcattagg ttcactcttg
    16501 ttcaaaatca ttttaagttc atgttcatca tgaagtacaa tgacatgatt atgtaacgtt
    16561 aacaatatac gctgtgcctc tgaagcaacg tttaactgtg cacgccctgt accatacaaa
    16621 tatattgttg aagctgtatg cattttttta gtaagaagct ggtaatcaac gcgttctaaa
    16681 taactgaatg tactatcgat ttcttgtttg aaactatcga tagagttaga aggtaattgg
    16741 tgtgtcttat tactttcaaa tttaaggtac gatttaaaat cactataacc gtcaaaacct
    16801 aatttgcgtg taaatctgtg aatggttgca ttcgaggcat gtgtgaattg cgctaaatct
    16861 tgtattttca tatttttaca ttcatctata tgtgtattga taaagtgagc gatatgtaaa
    16921 tcattatcat taagtttatc aaagtttcga ttaatgtgtt catcta
//
LOCUS       DAKTIP010000038        16728 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAKTIP010000038 DAKTIP010000000
VERSION     DAKTIP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.038"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(28..891)
                     /locus_tag="O0305_000982"
     CDS             complement(28..891)
                     /locus_tag="O0305_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0274135.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(933..1718)
                     /locus_tag="O0305_000983"
     CDS             complement(933..1718)
                     /locus_tag="O0305_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0274136.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(1844..2734)
                     /locus_tag="O0305_000984"
     CDS             complement(1844..2734)
                     /locus_tag="O0305_000984"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCY0274137.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(2744..4090)
                     /locus_tag="O0305_000985"
     CDS             complement(2744..4090)
                     /locus_tag="O0305_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0274138.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFYLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(4204..5304)
                     /locus_tag="O0305_000986"
     CDS             complement(4204..5304)
                     /locus_tag="O0305_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCY0274139.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKALLMNWFNTEKINLDVEASTINTDPFQYI"
     gene            complement(5307..5984)
                     /locus_tag="O0305_000987"
     CDS             complement(5307..5984)
                     /locus_tag="O0305_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCY0274140.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     ELIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(6115..7221)
                     /gene="rpoD"
                     /locus_tag="O0305_000988"
     CDS             complement(6115..7221)
                     /gene="rpoD"
                     /locus_tag="O0305_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCY0274141.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(7445..9244)
                     /locus_tag="O0305_000989"
     CDS             complement(7445..9244)
                     /locus_tag="O0305_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0274142.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPE
                     PIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDF
                     YAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIE
                     SLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(9305..10123)
                     /locus_tag="O0305_000990"
     CDS             complement(9305..10123)
                     /locus_tag="O0305_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCY0274143.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDINPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(10134..10757)
                     /locus_tag="O0305_000991"
     CDS             complement(10134..10757)
                     /locus_tag="O0305_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0274144.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHKVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            11092..12483
                     /locus_tag="O0305_000992"
     CDS             11092..12483
                     /locus_tag="O0305_000992"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCY0274145.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(12633..13379)
                     /gene="recO"
                     /locus_tag="O0305_000993"
     CDS             complement(12633..13379)
                     /gene="recO"
                     /locus_tag="O0305_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCY0274146.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYAALAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(13407..14306)
                     /gene="era"
                     /locus_tag="O0305_000994"
     CDS             complement(13407..14306)
                     /gene="era"
                     /locus_tag="O0305_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCY0274147.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(14307..14711)
                     /gene="cdd"
                     /locus_tag="O0305_000995"
     CDS             complement(14307..14711)
                     /gene="cdd"
                     /locus_tag="O0305_000995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCY0274148.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(14722..15066)
                     /locus_tag="O0305_000996"
     CDS             complement(14722..15066)
                     /locus_tag="O0305_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCY0274149.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(15069..15536)
                     /gene="ybeY"
                     /locus_tag="O0305_000997"
     CDS             complement(15069..15536)
                     /gene="ybeY"
                     /locus_tag="O0305_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCY0274150.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(15537..16484)
                     /locus_tag="O0305_000998"
     CDS             complement(15537..16484)
                     /locus_tag="O0305_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCY0274151.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         5258 a         3114 c         2248 g         6108 t
ORIGIN      
        1 ccgttctctt ttaaaatttt aatagcatca tttgtattca ttgatattag ctcccttttt
       61 aaacttcatt cgcattttct gataagccat tgtaatcata agtaaaataa caagtagaac
      121 tacaattaca ccacccggag aaatgtccat atagaaagct aggactaagc ctaatattac
      181 tgataattca cctaaaaata cacttagtaa tatcaattgc ttaaaacttt ttgttattcg
      241 catacttatt gcaattggta acgtgattaa cgcacttact aacagtatcc ctacaacacg
      301 cattgaggca gaaataacca tcgctacaat aacaataaat aaaaattgaa tccatttagg
      361 aatgccaatg actttactat attcctcatc aaatgacaat ataaataatt ctttataaaa
      421 caatgtaata aacagaacaa ctatgatggc aatgacaata atcgttgtta aatcacttat
      481 attcactgcg cttattgagc caaatagcaa tccaacaatt tcttgattga acccatcagc
      541 taatgaaatg aagattgcac tcaaggcgat accagcactc attataattg gaatagcaat
      601 ttcttggtaa gcagtgtatg acgttcttaa tttttcaatt agaagcgcac ctactattgc
      661 gaataagatt ccaaaccaca ttggattaat aaatactagt gttggcataa tagtaagtaa
      721 aaacataccg aaagatatac cacctaaagt tacatgactt agagcatcag ctataagtga
      781 tagtcgtcta acaacgataa aagcaccgat tagaggcgca ataaaaccta tcaagatacc
      841 actaattaaa gagtacctca taaaatcaaa attcaataat gcatctatca attgtgacac
      901 gcctttccat tttaaataaa ctcaaatctt tattaattac aacattctcg attatgctga
      961 tgatcgacaa aacgtacagg atgtccataa atttttgaaa tttcaacttc atcaagtgat
     1021 ttaaactcat cagttgtacc atggaaatgc aaatgcttat ttaaacatgc tacttcagta
     1081 gcagtatctg ctacaacacc gatatcatga gtaactaaga taatggtgat accttcttgt
     1141 tttaattgat ctaaagtatt ataaaattca cttacatgtt ttgcatcaat accattcgtt
     1201 ggttcatcaa gtactaatac tgcaggttct gaaatcaatg ctcgagcaat cattacacgt
     1261 tgttgttgac cacctgataa ttctgctata tttttatgaa ttaaatcact tatattcagt
     1321 ctttctagta ctttaatcac tttttcatta tctttgctat taaatgtttg gaaaagacgt
     1381 tttgtctttg ttaatccgct taaaacaact tctttaacac ttgctgggaa acctgaatta
     1441 aaggcatttg ctttttgtga tacatagctt aatttaattg atgttttctt atttttaaaa
     1501 tcaatacctt caacaaaaat ctcaccactt tgtaaaggta ataaccctag aatcaacttc
     1561 aataatgttg atttaccagc accatttggt ccaacaattg ctaaaaattc acctttattt
     1621 attttaatgt ttatattttc taacactttt ttatgatcat agtagtaatt gacatttttc
     1681 aattcaaaga ctggtgtcat cgtattctca cctcgcattc aactatacaa ctcctagtaa
     1741 catatgtaaa cagtaatgtt tacgactcaa aattagacaa aataaagaga tatgccccct
     1801 tcaagtttta tttatcgcat ttcttgaaga gagcattatc attttattgt tgcataacct
     1861 tattttttaa ttctgggtca aattgctgtt gttttaacat ttcaatttca agtttatatg
     1921 gcggtttttt atttttctta tcttcaccaa cataaggtgt ttctaagatt ttcggaatat
     1981 ctttaaaact atcatgatgc acaatgtaat ttaatgcatc aaaaccaatg taaccgaagc
     2041 caatattttc atgtcggtct ttttgagcgc cacggtcatt tttagaatca ttgacatgaa
     2101 caactttgat tctgtcgact ccaatgattt tatcaaattc atttaatacg ccatcaaagt
     2161 cctctttaac attatatcca gcatcatgcg tatgacatgt atcaaaacat actgataaac
     2221 gttcgttatt atgaactcca tcaataatac gtgctaactc ttcaaatgag cgaccaatct
     2281 ctgtaccttt acctgccatc gtttcaagcg caatacgtac attattgtca ttcgttaaaa
     2341 cttcatttaa tccttcaata atcttattaa ttccggcatc aacaccagct ccaacatgcg
     2401 cacctggatg taatacaata tctttagccc ctatagcttg cgttctttca atttcttgtt
     2461 gcaagaaatc tacaccaaga ttaaacgttt ctggtttggt tgtatttgca atattaatga
     2521 tgtatggtgc atgaacaaca atattagata aaccatattt ttccatcact tcatgacctt
     2581 tagttatatt taaatcttca atacttttac ggcgcgtgtt ttgaggtgca ccagtataaa
     2641 tcataaatgt tgtttcacca tattcatgcg cttctatagc agaaccttct aacatctttt
     2701 taccactcat tgaaacatgt gatcctaata acataaaaca cacctaacct tttttgtttt
     2761 gcttacgttt ttgtctattt tgctgcttac taaattgctt acgctcttga cgtttcattt
     2821 tttcaacttc ttgtttaaat ttcttcttat aacctggttt aactttgttt ttaattttac
     2881 ttcgaacttt gttcttcact tgattagtta aatggtcatc tttgcgcatt cttgcttgac
     2941 gctgattgtg cgctttaact tcttttaact caccatcttt aatatcaaca gtattgaata
     3001 caaaaccgcg atcttctatt aatgaaatat tgtgttcttc atcaggacta taaagcgtaa
     3061 ttgctacacc tttataattc ccacgaccag ttcgtccgac tctatgcgta aagaagtcaa
     3121 tatcatttgg cacatcaaaa ttgatgacat gactaacacc ttcaatatca ataccacgag
     3181 atgctaaatc gctggcaata acgtattgga attctaaatt acgtatacgt ttcatttgtt
     3241 gtttacgttc acgtggcgtt aagccaccat gaatcatacc aactttaata ccagcttcat
     3301 ttagtgaacg tgctaaatca tttgcattat ctctactatt acagaaaata atacataagt
     3361 atggatttag tatatcaatt aaatttaaag tcttttcaac tttagctgca cctttagtag
     3421 gtattaaata gaattcgatg ttctttttat tttgtttttt actgtcgaca gctacatatt
     3481 ctggatgact taaatattta tttaaaaatg gttgtaactg ttgtgggatt gtagcactaa
     3541 acaccgcaat atttgcatta tcttccaatc ttgcagcaat gtaatctaca tcttcaatta
     3601 atcctaagtc aatcataaga tccgcttcat caataactaa atatgatgct aagtgcacat
     3661 gtaaatgtcc cgttttagct aagtcattaa ttctagtagg ggtgcctata atcaattgtg
     3721 gttgtgcatt acaacgttgt ctatctttct ctatatctgt accaccaata aaaactttaa
     3781 ctgaaacacc agctttaaat tggcttaaat ggttcgctgc atcgtatagt tgttgtgcaa
     3841 gttctcttgt tggtgcaact acgattgctt gtggttcttt tatttcacta tcaattaact
     3901 gcattaatgg taataaaaat gcatgagatt tccctgtacc cgtttgagat tgaccaatta
     3961 aatttgttct ctttagtatt cttggaataa ttcgattctg aatttcagtt ggtttttcaa
     4021 aattaaggtc tttcacagcg tcaattaaac tagattctag attaaattgt tcgaatggat
     4081 gttttgccat gatttggcct ccttatatgt tcatctttac tattataaag tataaatgtg
     4141 ctattttaaa taaaaatatt aaaacctcga aatttcaaaa tttagaaatc ccgagatttt
     4201 agtttaaata tattgaaatg gatctgtatt aattgtagat gcttcaacat ctaaatttat
     4261 tttttctgta ttgaaccaat tcattagtaa cgcttttaaa ccttctttca tcacatattc
     4321 gctgtaatga ttaatatcaa ttaaattcac accatgaatt ttagcatcta aggcatcatg
     4381 atgtttaata tcacctgtaa caaagacatc tgcgccttgt tggacagctt gatattcata
     4441 tccaatacct gaaccaccaa taattgcaat acgtttaatt ttctgattag actcaccaac
     4501 aaaacggaca cttgggatat ttaatttaga tttaatatca gctgcgaaat cttccaatgt
     4561 catttgatta tccacttctg ccataacgcc aagtccataa agggatgttt gttttatctc
     4621 aataaaatca aataccggtg tttcatatgg atggtattgt ttaattaatt gctcagccct
     4681 tgacttttga tatgcatcta tcataaattc aattttaact tcatctacat attcaatttt
     4741 atcaatttgt cctattgttg gattagcttc accaactggt ttgaattgcc ctcttccttc
     4801 actttcaaag aaacaatatt cataattacc ttcttgcgct aatccatttt cactaagctt
     4861 atctttaaat ggtccaacat tatccttagg tatatatgtt tgaactttat agtatacatc
     4921 ttgttgatta tttattattg aaatgttctt caaacccatc gccttcgcca acatcatatt
     4981 gacaccatac ggatttacat ctaaatttgt atgcatcgct attaaattaa tgtcatgttg
     5041 aattagtttt ctaatgatca aaccataacc attagctttt aatgatgtta cgcctttaaa
     5101 gattagagga tgatgactaa taatagtatt ataacctttt tcgattgctt catttactac
     5161 ttccaacgta cagtctaatg ctgttaaaac accagtaact tcaacatctt catcacctat
     5221 taacaatcct acattatccc aagattcagc agtactaaat ggcacatgat gatctaacaa
     5281 tgtcattaaa tcagctattt tcattactat aacacccttt caattacagc aatttcgtca
     5341 ttaatttgag ctaaacgttg atgatgttgt tcagtattga gtttcgattt aatatgataa
     5401 agtgcttcta actctctttg ccattttttt ataaaatatt catttttgtt gttcagcaat
     5461 tttggtccga atttcaattc atcagatgat agctctatta attctgtaga atattctgct
     5521 acaacaattt cataaatatg gcctttttct tccattatta tttcatcaat tatttcataa
     5581 ttcaattgtt gtaatgtttg tcttaaattt tcagtttgga tattactttg taaaatcaac
     5641 cttggatgtt gacttaactt atcttgccca tcttttaaaa ttttagcaat aagtggtccg
     5701 cccataccac aaattgtgat attatcgatt acgtcctcag gttgaataac acttaagcca
     5761 tcccctaaac gtacatcaat tctatctact aattggtttg cagctacatt tttcacagca
     5821 gcttgaaaag ggccttgaat aacttctcca gcaataccgc attcgcataa atggttttga
     5881 attgcataga ttggcaaata agcatgatct gagccaatat ccgcgattgt accttgtttt
     5941 aaaaatcgac ttaccgtcgt taatcggtta tttaacgaaa tcattttatt ctcctttatg
     6001 aaaaaagagg ttgaacatta ttcaatgtac caacctcact caagttaaat cttaatctaa
     6061 aaataaataa gcgcttattc atgtcttggt atcaatcatt aaaattgata taaattaatc
     6121 cataaagtct ttcaaacgtt tactacgact tggatgtctt aattttctaa gtgcttttgc
     6181 ttcaatttgt cgaatacgtt cacgtgtaac accgaaaact ttaccaactt cttcaagtgt
     6241 tcttgttctg ccgtcatcaa gaccaaatct taatcgtaat acattttctt ctctatcagt
     6301 taatgtatca agcacatctt ctaattgctc ttttaataat tcataagcag catgatctga
     6361 aggactttgt gcttcctgat cctcaataaa gtctcctaaa tgactatcat cttcttcacc
     6421 aattggtgtt tctaatgaaa caggttcttg cgcaattttt aaaatttcac gaactttttc
     6481 tgctggtaaa tccatttctt caccaatttc ttctggtgct ggatctcgac ctaagtcctg
     6541 taataattga cgttgaacac gaattaattt attaattgtt tctaccatat gcacagggat
     6601 acgaatcgta cgtgcttggt cagcaattgc acgagtgatt gcttgtctaa tccaccatgt
     6661 tgcatatgtt gaaaacttaa atcctttgtt aaagtcaaat ttttcaacag ctttaataag
     6721 acccatatta ccttcttgga ttaaatcaag gaataacata ccacgaccta cgtatctttt
     6781 agcaatactt acaactaaac gtaagttcgc ttctgcaagt cttgattttg ctacttcatc
     6841 accttgttca atacgtttgg ctaattcgat ttcttcttgt gcacttaata agttaacacg
     6901 cccaatttct ttaaggtaca tacgaactgg gtcatttatt ttaacacctg gaggggcact
     6961 aagatcactt ggattcagtt tctcgtcagt atctgaacta tctttttcat taactagtga
     7021 aatatcatta tcatttaatt gatcaaagaa atcatccatt tgatcagagt cgatatcaaa
     7081 attctgaagt ttttcagcaa tttcttcatg acttaaatga ccctcttttt tacctttttc
     7141 aattaattgc ttcttaacat cttctaatgt taatgtcgga tcaattgttt gttttttaat
     7201 tttaactgtg ttatcagaca tgaaacggcc tcccgatttt aaatatgaac attcgaaatt
     7261 tattcaatat tgctatttta aactaaattc ttaattaatt catccatatt tttaatttta
     7321 ttttacaaat tggaactaat cccaatattt atttttcaat agtgttgttt aaatgcggta
     7381 actatcattt acccttatac attttgacat aatcattatt cgtattattc tttaaaatca
     7441 catgctacat gcgttcttta ttcttagcaa caatttgctg taaatagtat ttttgtaatt
     7501 ctacatcgcc aatccttgta gcttccctta atttatgatt caatgactca attgtttctt
     7561 gtcctttttc attaataaca ttgacataat catcaatttc attttcatat ggttcgtcat
     7621 tcaaattata ttgttctaag ctaattagtg tttctctcaa ctcatttgaa ttaacatact
     7681 gcacagcatc actgatatta tattgatcat tttccgcata aaaatcatgt aagacttcga
     7741 atacatattt aaaatgctga tttgtgaagt tatccttatc aacactttca taataattta
     7801 aaaatgtatc tttatctctc attaaatgtt ttaaaaatgc tcgctccgct ttttcttgac
     7861 ggctcaaatt gtcaaattgt gccataccaa ttggctcagg ttcaatgtaa ccgccatact
     7921 catcttctgg ataataattg gctggtgctt gattgaattg tatttcgtta gctaattgct
     7981 caggactaac attgaaaaat ggtgcaacat catttaaagc cttttgttgc aaaatcgatg
     8041 atttcataag cgaaatatca tgacttagtt ctttcaaata acgttcatat gaaaggtcat
     8101 tatgtgcaat ttcatctttt aatatactca ctttataatg tgcaaatgac tttttgtcat
     8161 tttttacaaa agcagtaaat gcatcgttgc catacttacc aatgtattca tccggatcca
     8221 tgcctgatgg caattgtata acaaatacat ttagcccttg ctgtaacaaa ttttgacctg
     8281 ttttaagtgt tgcttcacta cccgcaaaat ccccatcaaa cattaatgtt atatttgatg
     8341 ttaactttcg tataaaagta atatgttcat ctgacaactg tgtacccatt gttgcaacaa
     8401 cgtttttcaa gccagcagta tcagatttta taacatccat aaaaccttct agtaatacga
     8461 tttcatctaa ttttctaatt gatttacgcg ctttatctaa gttgtataac aactttcttt
     8521 tttgaaagat aggtgtttca ggactattta agtattttgg ttcttgaccg gtatatgttc
     8581 gacctgaata tccaacaatt cttccttgcg catttttcaa aggaaacata atacgatttc
     8641 gaaatctatc gtaataactg aaattttctt cgttacgtga taataatccg gcttcatatg
     8701 ctaattcaat atcgtaaccc tttttttgaa gaaaatcatg acaaaaatgt gagctatcgg
     8761 gtgcaaagcc aatgcctcgc tctttaataa gcgcatctgt aaaaccacgt tcttgtaagt
     8821 acgttaatgc ttgttcgcct tcgactgtct ttgttaaagc gtaataataa aattcttgta
     8881 ttaactcatg catttcaatc atttgtaaat catcagaagc aatttgaaca tttgagttag
     8941 attgtgttgc ctcaatatct acagcaacat taactctatc acctaattct ttaaccgctt
     9001 caacaaatga tatgtcttta atttcttgag taaattgaaa aacattgcca ccttttttac
     9061 aaccaaaaca atgacaaatt tgtttatctt cagaaactgt aaatgaaggt gtcttttcat
     9121 catgaaaagg acacaaacct atataattgc gtcctctctt ttctaatttt acatattcac
     9181 ttaccaagtc taaaatgtcg gttttatctt ttatttcatt aatgatcgat tgatctattc
     9241 gcaaattaaa tcacctatta agcattagtt atactattat acctaatttt cgacaaaaat
     9301 gaaatcattt cgatttattt tgttcaatat aatggattat atcgtttgct gtttcttcga
     9361 ttgctttttg agaaacatca atgacaggac atccaatttc acttacgatt tcttcaaagt
     9421 aattcaattc ttcttgaatt cgtgcttctg ttgcatatcg agctgtgtca cctagtccta
     9481 attgttttag tcgctctttt ctaatgcgat ttaatttttc ttcacttatt ttaagtgcga
     9541 tacatttctt tggattaata tcatataagc catctggcgg tgtcacttct ggtacaatcg
     9601 gtacattcat aactttgtaa ctcttatgcg ctaaatactg agataatggt gtctttgaag
     9661 ttctcgaaat accaagtaaa acaatatcag ctttaggtaa tcctttagga tctttaccat
     9721 catcatattt aactgcaaac tctatcgcat caattttctt gaaatatgca tcatctaatc
     9781 tatgaacgat acctggctca ttataaggtt tttcttcaac cgaagcagat aataaatcca
     9841 ttaatggccc catgatatcg acagacttca attggaattc tgctactttc tcactcatat
     9901 attgcttcat ttcaggttta ataagtgtat aaacaatgat agcatttgta tcttttgcaa
     9961 cttgaatcac ttcatcaaca tcttcaaaag attcaatata tggatatctt aataattcat
    10021 ttttacattg cttaggattg aattgtgaaa tacctgccct agcaactaac tctgccgttt
    10081 cacctataga atctgaagct acgataattt taattttttc cattacttct cacctattct
    10141 ttatataatg ctactaataa cttagctatt gttgttttgg aaattcttcc aattacttca
    10201 tacttttgat tatctttttt tcttacaatt ggaatcgaat caatttcttt ttcaatcatt
    10261 ctatctgctg cgtatatgac taattcgctt tcctctaaat aagtgacatt aggcatacgt
    10321 gtcatattta cactgatagg tactttatga atatctgctc caatcattga agctcttaat
    10381 aaatcttttc ttgaacacac accaacaaaa tcgttatctt cattaataat aaataatgta
    10441 cttacatctt ctaaaaaaat tgtacaaata gcgtcataaa ctgttgtatt ctctcttagc
    10501 acaacaggtt gagacatata gtccttaacc tcaaattgtc gaagtttttc attaaaaaat
    10561 ttgccttttg atttacctga ataataatat ccaactcggg gacgcgcttc taaaaaacct
    10621 gacattgtta atatcgctaa atctggtcta agcgttgctc ttgttaaatt caacttatct
    10681 gctatttgtt caccagtaat gggtccttta gttttaacaa tttcgatgat tcgttcttgt
    10741 ctttgactga gttctatagg tcttcacccc ttttttattc acctattatt ataccgatat
    10801 ttctttcatg tcacaaacac attaatttta cttgccttta aataatctat caattagaat
    10861 aaacgtacag cgagttaagg atagtgtaag cttaacaata agattggcgc aacgaatcat
    10921 tttaaaataa aagcgagtga ctacactaat ttgggtggaa ccgcgggtta aatacgtaac
    10981 ttattttgta ttatatacaa tttaaaatat taactcgtcc catggaagta aagcatattt
    11041 actatgctgt tattttccat ggggcgtttt catgtatgag gagaggtaat tatggcaaaa
    11101 gatatggata caattgtttc attagcaaaa cacagaggtt ttgtgttccc tggtagtgat
    11161 atttacggtg gtttatcaaa cacatgggat tatggtcctt taggtgttga attaaagaat
    11221 aatgttaaaa aagcttggtg gcaaaaattc attacacaat caccgtttaa cgttggtatc
    11281 gatgctgcaa tcttaatgaa tccaaaagta tgggaagctt caggacactt aaacaacttc
    11341 aacgacccaa tgattgataa taaagatagt aaaattcgat atcgcgctga taaattaatt
    11401 gaagattata tgcaagatgt taaaggtgat gaaaacttca ttgccgatgg tttaagtttt
    11461 gaacaaatga aaaaaattat tgacgatgaa ggtattgttt gtcctgtaag taaaactgct
    11521 aactggactg aaattcgcca attcaattta atgtttaaaa cattccaagg tgtaactgaa
    11581 gattctacaa atgaaatttt cttacgtcct gaaacagcac aaggtatttt tgtaaactat
    11641 aaaaacgtgc aacgttcaat gcgtaaaaaa ttaccatttg gtatcggtca aattggtaaa
    11701 tcattccgta atgaaatcac tccaggtaac ttcattttca gaacaagaga atttgaacaa
    11761 atggaacttg aattcttctg taaacctgga gaagaaatcg aatggcaaaa ttattggaaa
    11821 acttttgcaa gtgactggtt aacaagctta aatatgagca gtgaaaatat gcgtttacgt
    11881 gatcatgatg aagatgaatt atctcattac tcaaatgcaa caactgatat tgaatataaa
    11941 ttcccatttg gttggggtga gttatggggt atcgcaagtc gtacagactt cgacttacgt
    12001 aaacatgctg aacactctgg tgaagatttc agataccatg atccagaaac gaacgaaaaa
    12061 tatattccat attgtatcga gccatcactt ggtgcagatc gtgtaacatt agctttctta
    12121 tgtgatgcat atgatgaaga aggcgttgaa ggtagtaaag atgcacgtac agttttacac
    12181 ttccatcctg cattagcacc atataaagca gcgattttac ctttaagtaa gaaattatct
    12241 ggcgaagcga ttaagatttt tgagcaatta agttctaaat tctcaatcga tttcgatgaa
    12301 tcacaatcta tcggtaaaag ataccgtcgt caagatgaaa tcggtacacc ttattgtgta
    12361 acattcgact ttgattcatt agaagataat caagttacag tacgtgacag agattcaatg
    12421 gaacaagttc gtatgccaat ctcagagtta gaagctttct taactgaaaa aacaaaattc
    12481 taattaaaaa gactttccac catatgtggt ggctatataa tttataaaaa gagatactgg
    12541 ttagtaaaat tgccagtatc tcttttctat tcaaaaaggt attcaaataa acacatctat
    12601 gtggctactt gaataccttt tattatttta tgttattgtt ccaatctttt taattggttg
    12661 attagtttct gacttttaaa aaacatacct gcatattcac gatataacat taaaatgata
    12721 tctgacattt catcaataat ttcttgatgg atattcaatg aattcatttt atctatcggt
    12781 aatttttgta atacatctaa taaatatagt gttttattcg ataatataac tgcatgtaca
    12841 tctttagaag cctcttgcct tgaaatcgca ccgtcaaact taaaactata acctattaaa
    12901 tctgcttgtg tgtcattacc actcacagca cagcgattaa atgatgcagt aaaaccaaat
    12961 cgtttcatgc actttaacat aactacgact gacattaact gtgcagatgt acctgattct
    13021 attttttcaa gaacaaattg taataattga tagttatatg gtgcaatgtc accttcatcc
    13081 attgagcgct caatagtttc agctgccaga gcggcataac tgcttacgta aaggtccatt
    13141 tgtaatttat aatgttgact aataacatct acagaattta acgttcccat acctcgccac
    13201 tgattgtaaa taaacaaacc ataaacaaac aattgcgttt gcgcttgtaa acccgtctta
    13261 acttttttag cacgccttgc cataagtggt acttttgcac catgctcatt taaaatcgtg
    13321 ataattttat cagattcacc ataatcaact gctttgatga taatcccttt ttggcgcatt
    13381 aacaattatc ttcaccactt ttaagattaa tcttggtctt caacataacc aatttggcga
    13441 ataaagttaa ctttgtttcg ccagtctctt tgaactttga cccataattc taagtaaact
    13501 ttagagccta gaagcatttc tatatcacgt ctcgcacgtt ttcctacttc ttttaacttt
    13561 ttaccgcctt ttccaatgac aattcctttt tgcgaatctc tttcaacata tatagttgct
    13621 tcgatatgaa cacgatcttc gctttcttta accatacggt ccacattaac accaatcgca
    13681 tgagggattt cttcacttgt aagatgaagg attttttcac gaatgatttc acccactaca
    13741 aattgttcag gatggtctga aatttgatca tctggataat atttaggtcc ttcgggtaaa
    13801 tacgtcttta aaacatcaat aaaatgatcg acatttagcc cttctaatgc tgaaataggt
    13861 acaatctctg taaagtccat ataactttga tattcttcaa tctttggcat taattcatct
    13921 ggatgcacta aatctatttt atttaatact aaaaatactg gtgtcttaac atttttcaac
    13981 atttctataa tatattcatc gcctcgtcca atttcctcat tggcattaac cataaacatg
    14041 attgcatcta tctcagataa tgtattttta gcgactttca tcatatagtc acctaatttg
    14101 tgtttaggtt tatgaatacc tggcgtatca atgaatataa tttgcgcgtc atctcttgtc
    14161 ataacacctt gaattttatt tctagttgtt tgagctttat cggacatgat tgctatttta
    14221 tggccgatca ctctattaac aaatgttgac tttcctacat ttggtctacc tataattgaa
    14281 acaaatcctg atttatgttc tgtcatttat tctaaatcct ttcctgaaaa tccaaatggt
    14341 agtaactctg cgactgtcat cataaccata tctcctttat gatttgtcat atacacaggc
    14401 atatcatcat cacataattc cttcaaaact tgacgacatg caccacaagg tgatgacggt
    14461 ttatctgcat ctacggttac agttattgat tcaaaatcac ctggtctgta tccttgagaa
    14521 attgccgata ccaaactagc tcgttcagca catatcgata atggataaga agcattttct
    14581 acattggtac cataaaaagt tctaccgtct ttcgttttta aataagcccc tactttaaat
    14641 tgactgtatg gcgaatatga ttcttgttgt gcttttctaa cttcttgaaa ataatgaggt
    14701 tgataactca tatatgcctc cctaaaataa cgctataaaa tgtggtaaaa atactattaa
    14761 acctataata aatgctaata ttgaaactat aagtacacta aaagccgcaa tatctttagc
    14821 gtatttagct aaatcatgat attcaacggt cactaaatcg acaacatatt caatagcagt
    14881 gtttaaagct tcaacagtga gaactaatgc aatagcaatg agtataaata tccactcaat
    14941 ccgattaata tttagtacga gaccaaagac aatagcaaca atcattgcaa acacatgtaa
    15001 aagaaattta tagtcttttt gaattaagat tttcagccca tcaagtgcat atttaaacct
    15061 tttcataatt agtctcgtgt taatccatat gcgtttaata ttgtatcttg tcgaccaaac
    15121 atttcctttt catccgcttc agtcatatga tcataaccta atagatgcaa aaatccatgt
    15181 aatgctaaaa atcctaattc tcgttcaaaa gaatgtccgt aattgtttgc ttgttcttgc
    15241 gctacatccg tacagataat tatatcccct aaaacacgtg gtatatcaag accactaaaa
    15301 tcaatctctg gctcatcttc ttctaaagca aatgagatta catctgtaac tttatcttta
    15361 tctctatatg ttcgattaat ttcttgtatt tcttgtttat ctacaaatgt aacagaaagc
    15421 tcagcatcgt cttctatatg ctcttctttt ttagcaaatt ctaataaatc ttcaatttgt
    15481 ttataccaag catctttaac taagcctgtg tgatcgctaa aatctatcgt aaacatttaa
    15541 ttctctcctt cataatgttc aatgatctta cttaccaatg gatgtctgac tacatcgctc
    15601 tgatctaatt tcaatatact tataccttta acgttgtgta acctactgac cgcttcctta
    15661 agtccacttt taacaccttt aggtaaatcg atttgagttt ggtcaccagt aactaccatt
    15721 tttgagccaa aacctagtct tgttaaaaac attttcattt gcgcatgtgt cgtattctgc
    15781 gcctcatcaa gaattacaaa tgcatcttct aatgttcgtc cgcgcatata tgcaagtggc
    15841 gctatttcga taatgcctct ttcaataaat cgctctgttt gttcacgccc aagaacagta
    15901 tatagaccat catataaagg tcttaaatat ggatctacct tttctttcaa atctcctggt
    15961 aaaaatccaa gtgactctcc tgcttcaaca gcaggtcttg ttaatacaat acgtttaaca
    16021 gcacctttac ggagttgctt tgctgcataa actacagcta agaatgtctt acctgtacca
    16081 gcaggaccta taccaaatac taaatcatta tttttcatgg cattaacata tatacgttgc
    16141 cccatcgttt tcgcacgaat cgtcttacca aatgcatctt tagttatctc ttcatcatat
    16201 aaatctaaca gatgttgaat tgtgttatta tgcgccattt taatagctgc ttcaacatct
    16261 ttaattgtaa tattattacc taaatcaata accttcagca aattgattaa tactgattcc
    16321 gctttttcta cgttttctat ttttgtacct ttaacggcaa cttcttgtcc tcttgcatgg
    16381 atgacaacat cgaaactctc ttcaattgct tttaaatgtt catcattatt tccaattaaa
    16441 gcttgagatt ggttcatatc gtctatttgt ataattccag gcatacacgc gctccttttc
    16501 atatatcatt tatattcatt ctatgatgct ttaatatcta cttaattata tcatgcaatt
    16561 gattgattta aacatctttg cattgactta ctcatttttc tttaatttaa aataatcatt
    16621 tatacttttc accttgtcat aaaagcagat tcaattttag actaatcaat tttttgctgt
    16681 aatttaaata actaatattt ctcgaaaaat tctctttgtt ttttaatt
//
LOCUS       DAKTIP010000039        16115 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAKTIP010000039 DAKTIP010000000
VERSION     DAKTIP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.039"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(104..1153)
                     /locus_tag="O0305_000999"
     CDS             complement(104..1153)
                     /locus_tag="O0305_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP synthase"
                     /protein_id="HCY0274152.1"
                     /translation="MIPTLEEIIDKYGNLVDYLKFDVTVEVYEDLLLLRSLSDLNEHQ
                     KMDRVSFIKKHLNSNFDYLLDDTEFRSINKIYSNLNIMTHINSQNNNFKRNPFINEEQ
                     LKSLLAIKKVDAHMSYDSNITSKALAEINKTQKRLVTKIDTLYNQSKKENEYVRLGEK
                     ISYTKLENHWKYLYNEIQFYNLNNQLISYVALEQEYAWAFLNELFYLIELYLKAFKGQ
                     KKTDYEFGKKLNEFIQSYVIILLIDIRLPVVRLYIIRDIVDTCEGEKDNYKRITLIEE
                     SIKKYRYVKDQIKRFKNGLEESLLNATLSIEQNMLKVKIDYYKAYCFPREKTKHNNIE
                     YNVSLFFKALDALKK"
     gene            1292..1582
                     /locus_tag="O0305_001000"
     CDS             1292..1582
                     /locus_tag="O0305_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274153.1"
                     /translation="MNKKSKQQEKLYNFIIAKSFQQPVGSTFTYGELRKKYNVVCSTN
                     DQREVGRRFAYWIKYTPGLPFKIVGTKNGSLLYQKIGINPCNNSTPSKGGDC"
     gene            1582..3369
                     /locus_tag="O0305_001001"
     CDS             1582..3369
                     /locus_tag="O0305_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCY0274154.1"
                     /translation="MSYTLFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNA
                     RFSDPITGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQNW
                     TPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            3603..4952
                     /locus_tag="O0305_001002"
     CDS             3603..4952
                     /locus_tag="O0305_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0274155.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            4974..6602
                     /locus_tag="O0305_001003"
     CDS             4974..6602
                     /locus_tag="O0305_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0274156.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            7124..7474
                     /locus_tag="O0305_001004"
     CDS             7124..7474
                     /locus_tag="O0305_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274157.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7561..7872
                     /locus_tag="O0305_001005"
     CDS             7561..7872
                     /locus_tag="O0305_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCY0274158.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7887..8393
                     /locus_tag="O0305_001006"
     CDS             7887..8393
                     /locus_tag="O0305_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0274159.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            8445..10052
                     /locus_tag="O0305_001007"
                     /pseudo
     CDS             8445..10052
                     /locus_tag="O0305_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(10043..10303)
                     /locus_tag="O0305_001008"
     CDS             complement(10043..10303)
                     /locus_tag="O0305_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0274160.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(10275..11261)
                     /locus_tag="O0305_001009"
     CDS             complement(10275..11261)
                     /locus_tag="O0305_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0274161.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            11358..13367
                     /gene="mecA"
                     /locus_tag="O0305_001010"
     CDS             11358..13367
                     /gene="mecA"
                     /locus_tag="O0305_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0274162.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(13413..13841)
                     /locus_tag="O0305_001011"
     CDS             complement(13413..13841)
                     /locus_tag="O0305_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0274163.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13938..14681)
                     /locus_tag="O0305_001012"
     CDS             complement(13938..14681)
                     /locus_tag="O0305_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0274164.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(15518..15889)
                     /locus_tag="O0305_001013"
                     /pseudo
     CDS             complement(15518..15889)
                     /locus_tag="O0305_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            15943..>16115
                     /locus_tag="O0305_001014"
     CDS             15943..>16115
                     /locus_tag="O0305_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0274165.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPI"
BASE COUNT         5865 a         2697 c         2448 g         5105 t
ORIGIN      
        1 gatttccaca acatttttcg tatttcagaa cgattaagac tacattatcc tggcttaata
       61 ttaattaatt ttaattatat ctataaatat tttcagtgaa aaactatttc tttaaggcat
      121 ctaatgcttt aaaaaacaac gatacattgt actcaatatt gttatgcttt gttttttctc
      181 taggaaagca gtaggcttta taataatcaa ttttaacttt caacatattt tgttcaatag
      241 ataaagttgc atttaataac gattcttcta atccattttt aaatctttta atctgatctt
      301 ttacgtatct atacttttta atagattctt ctatcagtgt aattcttttg taattatctt
      361 tttctccttc acatgtatct acaatgtctc taataatata taatctaaca acaggtaatc
      421 gtatatctat aagtaaaata atgacatatg attgaataaa ttcgtttaat tttttcccaa
      481 actcataatc tgttttcttt tgtcctttaa atgccttcaa atacaattct atcaaataaa
      541 atagttcatt taaaaaagcc caagcatatt cctgttctaa tgcaacataa ctaatcaatt
      601 gattattaag attataaaac tgaatctcat tatataaata tttccaatga ttctccaatt
      661 ttgtatacga tattttttct cctaaccgta catattcatt ttcttttttt gactgattat
      721 agagtgtgtc tatctttgta actaatcttt tttgcgtttt gtttatttct gccaatgctt
      781 ttgaagtaat atttgaatca taactcatat gtgcatctac tttctttatc gcgagtagtg
      841 atttcaattg ttcttcattt ataaatggat tacgtttaaa attattattt tgactattga
      901 tatgcgtcat gatatttaaa ttactgtaaa tcttattgat acttctaaac tcagtatcat
      961 ctaataaata atcgaaattt gagtttaaat gtttttttat aaatgaaact ctatccatct
     1021 tttgatgttc atttaaatcg cttaaacttc ttaataacaa caaatcttca taaacctcaa
     1081 ctgttacatc aaatttcaaa taatcaacta agtttccata cttatcaata atttcttcta
     1141 gcgtaggtat caatatatac cccccctccc cattttttgt tccgcgtcta atcataatgc
     1201 ttcatttatg cttattatag caatcaattg aagatagttt aacctatatg atattgattg
     1261 actccttaaa aattattgga ggaattttaa aatgaataag aaatctaagc agcaagaaaa
     1321 gctttataat tttataattg ctaaatcttt tcaacaacca gttggaagta cgttcactta
     1381 tggtgaatta agaaagaaat ataatgtggt ttgtagcacg aatgatcaac gtgaagtagg
     1441 acgtcgtttt gcttactgga ttaagtacac gccaggatta ccatttaaaa ttgtaggaac
     1501 aaaaaatggc agtttattat atcagaaaat agggataaac ccatgcaata atagcacgcc
     1561 ttcgaaagga ggtgattgct aatgagttac acattgtttg aaattggccc atttgcttta
     1621 gattccttcg gttggaaaga aacgatacca cctaaaaaag atggtgacag tgaaaaagtg
     1681 gttagaatgg ctagccctat tattgttaat gcaagatttt ctgatccaat tactggtgtt
     1741 gaaaagttga ttattactaa caacaatggt aaaaaagaca tttttgagtc tgatatctta
     1801 acaacacgaa acttaccttc tttaattaaa tatggctaca gtattaacga gaagtatatt
     1861 agatcattga gttatgcctt gcaattgatg cgagatagat taccactttc tgaattatat
     1921 gagggtgtag gtatactaga gacacctttt ggctacttga tatcgctaga taaagttctc
     1981 aaatctattc aatttaatca gtcctcacct tcatacccta ttgtagatag tgcttatgac
     2041 ttaactccta aaggtacatt tgataattgg tttaacatgt atattgacga ggttaaaggt
     2101 catcttcttt tagaattggc agtcatattt gggatttctg ccttagtgac aagttttctg
     2161 aagcataaac atgaaatcga atttgcagga attctcttta gttttacagg tcaatcctca
     2221 accggtaagt caaccgcagc tgctttagct gtatcagtcg ctggtaaccc taccaaaggt
     2281 aatgaaactc tttttagaag ttggaatgcc acacgaaacg cacttgaagg ttatttgagt
     2341 aacaattatg gcattcctat agtatttgat gagctttcat ctacgacact tagagatacc
     2401 acgggtttgt tatattccat cgctgaaggt caaggtcgtc aacgttcaaa cgttcatgga
     2461 gaagttaaaa cgcctaagaa ttggggtact tctgtcattt caacgtcaga atacagtatt
     2521 tttaatgatt cagctcaaaa cgatggatta cgtgtaagaa ccatcgagat taatgaacaa
     2581 ttcactacaa atgcgaccaa tgctgacaat attaaaaaag ctgtagcact aaactatggt
     2641 catgttttac cgttagtagc taaatatctt ataaatcgtg aagatgaagt tattcaatgg
     2701 ttttacaagg aagtagattg gtttgaagct aaattgaaag atgaaacaaa caatacaggt
     2761 attcgtatgt ttaagcgtta tgcagtcatt acaacatctg cgaaaatatt aggtcgtgtt
     2821 ttatctacag atattgatat cgctaatata agagactact ttattgatta tcatactcat
     2881 acagtctctg aacgctcatt agctgataaa gctattgatg tcatcattca atttgtggca
     2941 caaaacagag gtaagttttc agatgaaggt gctttgaaaa atatgtttga gaactatgga
     3001 ttaatctcat taaaagatga ccatatcgaa gtaaaaataa ttgcgaacgt ttttaaacag
     3061 atgctaaaca atcatcaatt tcaagatgtt aataatgttg tcaacgcctt aagagataaa
     3121 ggtttcatat tagctgaccg tggacgccaa acaactaaaa gaagtgtcaa agataatagc
     3181 ggtaaaaaac aatcacttgt attttaccat ctaaaattag atgtagagtt tgcatctatc
     3241 ctcggattaa ctaaagataa gtcgttactt caaaattgga caccgtcaaa tgataacaaa
     3301 gctgcgaaag aactttttaa atccgctaat gaagggatag gcccttcagg agttcacgaa
     3361 gatttctaat tgtctttatc atacaactgt gaagcatcaa actgaataat cgacgtattg
     3421 aagatagaac aaataacgga gtgaaaaaca tggatgaact tatcgagata ttagccgatt
     3481 tggtaattga agaaatcaat caaacaaatg actaatatgg acgcccaaca tctcattgat
     3541 gttgggctta tatatcaatt catccataaa tatatattat cactgtgaaa ggaggagccg
     3601 atatgaaaca agtcataggc tatttacgtc aaagtacgat gaaacaacaa tctcttgcag
     3661 cacagaaaca agctatcgaa gcaatagccg aaaaacatca tattcaacat atcaactttt
     3721 atagcgacaa acaatcaggt cgcaaagata atcgtagtgg gtatcgacaa ataacacaat
     3781 taattcaaca aggacagtgt gacatattat gctgttatcg tcttaatagg ttgcatcgta
     3841 atctgaaaaa tgcattaaaa ctcatcaaat tatgtcaaac gtaccatgtt catatcttaa
     3901 gcgtacatga tggttacttt gatatggatc aagctttcga ccgactcaag cttaatatct
     3961 tcatcagttt agccgaactt gaatcggata acattggaga acaagtcaga aatgggcttc
     4021 aagaaaaagc aaagcaaggt cgattgatta caacacatgc accttttggt tacgaatatc
     4081 acaacggaac attcatcatc aatcaaaatg agtcaccaac ggtaaaggct gtattcaatt
     4141 attacattaa aggtcatggt tataagaaaa ttgcacagtt attagaagaa gataacacgt
     4201 atatcaatcg acaaccctat caagttcgta acattatcat caatcctaat tattgtggtc
     4261 gtgtcaacaa tcaatatggc caattcgaca atatgtttcc ttctattgtt tccacaagta
     4321 tatatgagca agcgcagaga cttcgattgc aaaaacaaac caaacagaca ccttcggata
     4381 atcaactcaa acaaaaaatc aaatgcccat gttgtaatgc aacacttaca aatatgacca
     4441 ttagaaaaaa gaatcataca ttacgttact acgtctgtcc taaaaacatg aatgcttcac
     4501 gctttgtctg tgattttaaa ggcatcaatg cacaaacact tgaagataaa gtattagaag
     4561 tgtgccgaga cttttatcaa aatcaacgca tctacacaaa aattaaaagt gcgattgaca
     4621 aacgcatcaa aagacaaaga aacatagaaa aacatcacac attgactcaa gaacaactga
     4681 tagaaaagtt ggcacaaggc atcattgatg cagaaacgtt cagagagcaa acacaatcat
     4741 tacgtcaaca accgcaacgc actacatcta tcaatgggca tcaaatacaa cacatcattc
     4801 aaaatattat tcaaaaacgt ttcacgttaa acatattgta cccctatatt gaaaccattc
     4861 acattacgaa agataaaaat cttataggaa tctatttcaa aaatgaacca ctcaatattg
     4921 tcaatcaaac catgcaatca tcgattgcat aaatgaagaa aggatgtaac ataatgcaac
     4981 aacttaaaac aaaacgtgtc ggtatctatg tacgtgtatc aacagaaatg caaagcacag
     5041 aaggttatag tatcgacgga caaatcaatc aaatcaaaga atactgtgac ttccatcatt
     5101 ttgaagttaa agatatatac gctgaccgtg gtatttcagg taaatctatg aacagacctg
     5161 agctccaacg tatgttgaaa gatgcaaaag aaggcaatat cgactgtgtt atggtataca
     5221 aaacaaaccg attagctcgt aatacatctg atcttctcaa aattgtcgaa gatttacaca
     5281 aacaaaatgt cgaatttttc agtttgtcag agcgtatgga agtcaatact tcttctggta
     5341 aactcatgtt acagatactt gcgagtttct cagaattcga acgtaataac attgtcgaga
     5401 acgtatttat gggtcaaacg agacgtgccc aagaaggcta ttatcaaggc aatttaccac
     5461 taggttatga caaaatacca gatagtaaac acgagctaat gattaaccaa catgaagcta
     5521 atattgtaaa atatatattc gagtgctatg ccaaaggaca tggctatcgt aaaattgcca
     5581 atgcattgaa tcacaaagga tatgtcacta aaaaggggaa acctttcagt attagttcaa
     5641 tcacatacat cttagctaac cctttctata tcggcaaaat tcaatttgcg aaatacaaag
     5701 attggagtga aaaacgtcgt aaagggctga atgataaacc agtgatagct gaaggtaagc
     5761 attcccccat tattaatcaa gatttatggg ataaagtaca aatgcgtaag aaacaagtca
     5821 gtcaaaaacc ccaagtccat ggcaaaggaa cgaatctgct tacaggcatt attcactgtc
     5881 cccaatgtgg cgcacctatg gcagcaagca ataccacgaa tactcttaaa gacgggacta
     5941 agaaacgtat tcgttactat tcatgtagta attttcggaa caagggttcc aaagtatgtt
     6001 cggcaaacag tgtaagagct gatgtgattg aagattatgt gatgaagcaa atacttgaaa
     6061 tagtcaaaag tgataaagtc attcaacgcg tagtaacaca cgtcaatcaa gaaaatcaag
     6121 ttgatggcgc tgcacttcat cacgatattg cttataagca acaacaatat gatgaagtac
     6181 aaatcaaaat aaataacttg attaaaacca tcgaggataa tccggactta acatcagtaa
     6241 tcagaccaag tattcaaaaa tatgaaaagc aactcaatga cattacgaat caaatcaacc
     6301 aactcaaaaa tcaacaaaat gaagataaga ctttatttga tgccaaagaa atcagtaaac
     6361 tattacaaca catctttcat gatattaagc acatagaaaa atctcgactc aaagcattgt
     6421 atctatcagt gattgatcgc attgatatta aaaaagatgg taatcataaa aaacaattct
     6481 atgtcacact caaacttaat aacgaaatca ttaaacaact tttcaataat aaacaactcg
     6541 acgaagtgca tctcagcact tcgtctttat ttttgcccca aacactatat cttactattt
     6601 agctaataga atcattgtta taacaataat tttcactata ttctttcgct tattttatct
     6661 aactattttc aagaattgct ctttatcaat ttcaataaca cttatctaat gaaacagtta
     6721 ttgtcgtaat tttttcataa ctatacaatg cttatattga aattgctaaa gaacatccca
     6781 caatgatttt ttatcgggaa atgattggga tttgcagatt cgaagaaaca gaaggattgt
     6841 gcaagcacaa tcccaaaatc ccgttacttt ttataaataa ataatatata taaggaagca
     6901 gagacacaac cctatcatca atacttataa ataatgagtt gattgtttgt caaaataggc
     6961 ggaatttcag gatttccttg gcataccaac gtttgtagcg ggtttttata tggaattttc
     7021 tcttattccc accgggattc gtaataaatg acaaatcaaa tatatattat ctcaatgatg
     7081 tatatgaact acatcactat tacactatct aggaggcaat aatatgaaaa ccatcactca
     7141 agaactcaaa caatatataa cccacctatt ccaactatct aataacgaag cttgggaatg
     7201 tgaagcgctg gaggaagcag cagaaaatat tctaccggaa cgttttatta atgattctcc
     7261 actggtacat ctcacactcg agacttatac ctactataat gatgaactac atgagctaag
     7321 tatctatcca tttctaatgt atgccaataa ccaactcatc agtgtcggat acttagacca
     7381 ttttgacatg gactttctgt accttacaga tactcaaaat atcattattg atgaacgtca
     7441 tttactaaaa caaggaggtc aggaccatga ataattggat catagttgct caattatccg
     7501 tcacagttat caacgaaatc atcgacatca tgaaagaaaa gcagaaggga gggaaatagc
     7561 atgaaaatca atcgatacat cacaagagga attaatgaaa gtattccact agaccttcaa
     7621 atcttacttt ggcacatggt agaaaaaaaa gataaccagc ctcatacaga ttacctacac
     7681 atttttaaat tacaagaaga tgagaatata ctctcaatca cacatgaaca agaacaaccc
     7741 acatacaaat tggaatatca ctatacaaac tatgaaaaaa atcaaaatgc attacctaag
     7801 aaagtctacg tcattcgtga agatgacgtg gacgcttttt attatgtgat gcttttacca
     7861 gaagaatact aaaggaagtg acgatgatga atacaatcaa aagtacgata cacacagaag
     7921 ccatatttag cgatgatgaa cagcaccgct atttactcaa gaaaacatgg gatgaaaaga
     7981 aagctgcatg cacagtgata acgatgtatc ctcatttaga cggtgtatta tcactcgatc
     8041 tcacaactgt tcttatcctc aaccaattag ccaattcaga acgatacggt gctgtatatc
     8101 ttgtaaatct attctctaat attagaacac ccgaaaacct caaacatatc aaaaatccat
     8161 acgatgagca cactgatatt catttgatga aagcgattag tgaaagtgac acagtgattc
     8221 ttgcttatgg tgcctatgcg aagcgaccag ttgttatcga ccgtgtcgaa caagtgatgg
     8281 aaatgttaaa acctcataaa aagaaagtaa aaaagctcat caatccagta acaaatgaaa
     8341 ttatgcatcc actcaaccct aaggcacgtc aaaaatggat tttgaaatca tagcaaatac
     8401 atggaggtca atatggaaac aaaacaaatc aacatcgttt tactgtaaat gatattgttg
     8461 aaacaattcc tgacaatgaa ttcgacgaat tcagacatca ccgtggtgca acttcgtacc
     8521 atcctaaaat gatgttaaaa gtgattctat atgcctacac acaatctgta ttctcaggtc
     8581 gtaaaataga aaaaatgctt aatgatagca tccgaatgat gtggctatca caaaatcaaa
     8641 aaccttctta taaaacaatt aatcaattta gagtaaatcc aaaagtagat gctttattag
     8701 aatctttatt tattcaattt tacagtcagt gtgtaaaaca aaatcttata gatgataaag
     8761 ctatttttat tgatggtaca aaaattgaag caaatgccaa tcgatataca tttgtatgga
     8821 aaaagagtat tcaaaaccat gaatcaaaga tgaatgagga ttctaaagcc ctctaccatg
     8881 aattggtaac caataaaatc ataccggaaa ttaaagaaga tcatgataat gaattaacaa
     8941 aagaagaaat agatttgatt ggtagtcact tagataaaga aatcgaagat ttaaaccaac
     9001 atatcaacaa tgaaaaatgt actaaaacaa gaaaacaaat acgtctcaaa agaactaaaa
     9061 tcaaaaaata caaaaagcaa atcaatgatt attttgagcg aaagtatcga tacgaatttc
     9121 aaaaatctat tttaaaggat agaaatagtt attctaagac agattatgat gcgacattta
     9181 tgagaatgaa agaagatcac atgaaaaatg gacaacttaa gccagggtat aatttacaaa
     9241 tagcaacaaa ttcccaattt gttttatctt ataatgtgta tcaaaatcca acggatacta
     9301 gaacgatgat tccattttta aattcaattc aagagaccta cggtcattta cctgaatata
     9361 ttgtagctga tgcaggttat ggtagtgaat caaattataa ggcaattata gatgacttta
     9421 atcgaacgcc actcataaca tatggaatgt ttataaaaga taaaactaaa aaatataaaa
     9481 gtgacatctt taatactcaa aattggaact atgacgaaat taatgacgaa ttcatttgtc
     9541 cgaataataa acggctaggt tttaaaagat atgcctatcg tcatgataag tatggttata
     9601 agcgagactt caaattatat gaatgtgatg attgttcaga atgtcctctg aaaaatcaat
     9661 gtatgaactt caattcaaaa acaaacaaaa aaataatgaa gaattataac tgggaatatt
     9721 ttaaatccca aattaacaaa aagctttcag aaccagaaac aaaaaatatc tacagtcaaa
     9781 gaaaaattga tgtggaacct gtttttggat ttatgaaggc tattttgggt ttcactcgga
     9841 tgtctgtccg aggactcaat aaagtcaaaa gagaacttgg ttttgtatta atggcactta
     9901 atataagaaa agtagtagct caacgagctg aaaataatca aaaaatttat aaaaaagaca
     9961 atttctatat tatttcaata gaaattgtct tttttcactt atccaagaac tttatgtccc
    10021 ggactcatta tgatttattt ttttattgct gacttgccat taattctgct tgctctacaa
    10081 cttgttccac agccattttt tgaagatctg gtggatagcc atatttcttt aataggcgtc
    10141 taacttgaac tctcatttta gctttagcac tgtctcgttt agaccaatca acactcatgt
    10201 tatccttaac tgttttagtt aactcatgtg caatggctct taactcttta tcgcccaaag
    10261 cttctttagc tgtttcatgt gaagctcgat atactacata caatatacgc aacaatacgc
    10321 ttgtttcgat taggtttagg cattgaatca taaagtgcaa tatacttaac acgttcttta
    10381 atatttgaat taaaacctag taaatattgt gctgccacat tatttatgtg ctgagatttt
    10441 aaaatagagc attttaatat cgattcacca taacgtatat gttcatggcg attcaaaatt
    10501 tttaaaacgt ttctatcaca tactttttca cagtcattgt ccatcattgt tttacttata
    10561 tatagtgcag gattaaacca gaatatcatt ttaaaaacaa cataaagctg gttgaatatt
    10621 aagtcatgac ttttcacatg tgatagttca tgtagaataa tatattcaat ttctttgtca
    10681 ttcatggttt cgactacgac agttggtagt acaatttggg atttcactaa accaaatacc
    10741 atcggattat caatgtttga actataacta attgttatat gctttttgta gaactgcatc
    10801 ttactttgac atactttaag tcgttcatta agatatgacg attccaatga cgaactttta
    10861 ataacatcaa tttgtcggaa tgccttaatc atataaaata agcacaacaa actaccaaat
    10921 acccatatca aaagaatcat atacgttata tttgaggtct caaactgatt aacattaatt
    10981 gctaagtctt tcgtaacaga tgattgttga ccatctaaca tatgactaac cgaagaagtc
    11041 gtgtcagata catttcgatt catcatatct tttgaaaatg taaaattcga tattttgtaa
    11101 aatggtatta atggaattaa cgtggagacg agcactaata accaaatctt atgtgacata
    11161 atattttgag tatattttat atagagcatt ctcactaaaa aaattacaca tatcgtgagc
    11221 aatgaactga ttatacttaa cattaaaaaa gatgataaca ccttctacac ctccatatca
    11281 caaaaattat aacattattt tgacataaat actacatttg taatatacta caaatgtagt
    11341 cttatataag gagtatattg atgaaaaaga taaaaattgt tccacttatt ttaatagttg
    11401 tagttgtcgg gtttggtata tatttttatg cttcaaaaga taaagaaatt aataatacta
    11461 ttgatgcaat tgaagataaa aatttcaaac aagtttataa agatagcagt tatatttcta
    11521 aaagcgataa tggtgaagta gaaatgactg aacgtccgat aaaaatatat aatagtttag
    11581 gcgttaaaga tataaacatt caggatcgta aaataaaaaa agtatctaaa aataaaaaac
    11641 gagtagatgc tcaatataaa attaaaacaa actacggtaa cattgatcgc aacgttcaat
    11701 ttaattttgt taaagaagat ggtatgtgga agttagattg ggatcatagc gtcattattc
    11761 caggaatgca gaaagaccaa agcatacata ttgaaaattt aaaatcagaa cgtggtaaaa
    11821 ttttagaccg aaacaatgtg gaattggcca atacaggaac agcatatgag ataggcatcg
    11881 ttccaaagaa tgtatctaaa aaagattata aagcaatcgc taaagaacta agtatttctg
    11941 aagactatat caaacaacaa atggatcaaa attgggtaca agatgatacc ttcgttccac
    12001 ttaaaaccgt taaaaaaatg gatgaatatt taagtgattt cgcaaaaaaa tttcatctta
    12061 caactaatga aacagaaagt cgtaactatc ctctaggaaa agcgacttca catctattag
    12121 gttatgttgg tcccattaac tctgaagaat taaaacaaaa agaatataaa ggctataaag
    12181 atgatgcagt tattggtaaa aagggactcg aaaaacttta cgataaaaag ctccaacatg
    12241 aagatggcta tcgtgtcaca atcgttgacg ataatagcaa tacaatcgca catacattaa
    12301 tagagaaaaa gaaaaaagat ggcaaagata ttcaactaac tattgatgct aaagttcaaa
    12361 agagtattta taacaacatg aaaaatgatt atggctcagg tactgctatc caccctcaaa
    12421 caggtgaatt attagcactt gtaagcacac cttcatatga cgtctatcca tttatgtatg
    12481 gcatgagtaa cgaagaatat aataaattaa ccgaagataa aaaagaacct ctgctcaaca
    12541 agttccagat tacaacttca ccaggttcaa ctcaaaaaat attaacagca atgattgggt
    12601 taaataacaa aacattagac gataaaacaa gttataaaat cgatggtaaa ggttggcaaa
    12661 aagataaatc ttggggtggt tacaacgtta caagatatga agtggtaaat ggtaatatcg
    12721 acttaaaaca agcaatagaa tcatcagata acattttctt tgctagagta gcactcgaat
    12781 taggcagtaa gaaatttgaa aaaggcatga aaaaactagg tgttggtgaa gatataccaa
    12841 gtgattatcc attttataat gctcaaattt caaacaaaaa tttagataat gaaatattat
    12901 tagctgattc aggttacgga caaggtgaaa tactgattaa cccagtacag atcctttcaa
    12961 tctatagcgc attagaaaat aatggcaata ttaacgcacc tcacttatta aaagacacga
    13021 aaaacaaagt ttggaagaaa aatattattt ccaaagaaaa tatcaatcta ttaactgatg
    13081 gtatgcaaca agtcgtaaat aaaacacata aagaagatat ttatagatct tatgcaaact
    13141 taattggcaa atccggtact gcagaactca aaatgaaaca aggagaaact ggcagacaaa
    13201 ttgggtggtt tatatcatat gataaagata atccaaacat gatgatggct attaatgtta
    13261 aagatgtaca agataaagga atggctagct acaatgccaa aatctcaggt aaagtgtatg
    13321 atgagctata tgagaacggt aataaaaaat acgatataga tgaataacaa aacagtgaag
    13381 caatccgtaa cgatggttgc ttcactgttt tattatgaat tattaataag tgctgttact
    13441 tctcccttaa atacaatttc ttcattttca ttgtatgttg aaagtgacac tgtaacgagt
    13501 ccattttctt tttttatgga tttcttattt gtaatttcag cgataacgta caatgtatta
    13561 cctgggtata caggtttaat aaatttaacg ttattcattt gtgttcctgc tacaacttct
    13621 tctccgtatt taccttcttc tacccataat ttaaatgata ttgaaagtgt atgcatgcca
    13681 gatgcaatga tacctttaaa tctactttgt tctgcttttt ctttatctat atgcatatat
    13741 tgaggatcaa aagttgttgc aaattggata atttcttctt ctgtaatatg aaggcttttt
    13801 gttttgaatg tttctcctac tataaaatca tcgtatttca tatatgtctc tctttcttat
    13861 tcaaattaat tttttagtat gtaacatgtt aaaggtaagt ctaccgtcac tgaaacgtaa
    13921 gactcacctc taactttcta ttgagacaaa tgcaccattt tatctgcatt gtctgtaaag
    13981 ataccatcaa ctccccaatt agcaagttgg tttgcacgtg ctggtttgtt tacagtccat
    14041 acgttcaatt cataacccgc ttcttttacc atttttactt ttgctttagt aagtttggca
    14101 tcttcagtgt ttactatttt agcattacag taatctaaaa gtgttctcca gtcttcacga
    14161 aacgaagttg tatggaatat aactgctctg ttatattgtg gcatgatttc ttctgcaagt
    14221 ttaacaagca caacattaaa gcttgaaatg agcacttctt gattctgatt taagtttgtt
    14281 aattgttctt ccacttgctt aaccatactt ttagaaagtg ctagtccatt cggtccagta
    14341 atacctttta attctacatt taaattcata ttatattcat ttgctatttt tactacatca
    14401 tcgaaagttg gcaaatgttc atctttgaat ttttcaccaa accaagatcc tgcagaagca
    14461 tctttaattt catcataatt caattcagtt atttccccgg acatatttgt agtccgttct
    14521 aaataatcat catgaatgat aatcagttgt tcatcttttg taattgcaac atctaactcc
    14581 aaccagttta taccttctac ttctgaagca gctttaaatg atgcaattgt attttccgga
    14641 gctttactag gtaatcctct atgtccatat acagttagca tattacctct ccttgcattt
    14701 ttattttttt aattaacgta actgtattat cacattaatc gcacttttat ttccattaaa
    14761 aagagatgaa tatcataaat aaagaagtcg atagattcgt attgattatg gagttaatct
    14821 acgtctcatc tcatttttaa aaaatcattt atgtcccaag ctccattttg taatcaagtc
    14881 tagtttttct gtacccctta tctgcaattt tacttaggat tgcttttaac ttacccctta
    14941 tcagcaattt tactgagaac tgcttttaac gcacctctta tctgcaattt tgcctagaac
    15001 tgcttttaac gtacctctta tctgcaattt tactgagaac tgcttttaac ttacccctta
    15061 tcagcaattt tgcatggaat tgcttttaac gtacctctta tctgcaattt tacttagaac
    15121 tgcttttaac aaacctctta tctgcaattt tacttagaac tgcttttaac gtacctctta
    15181 tctgtaattt tactgagaac tgcttttaac aaacctctta tctgcaattt tacttagaat
    15241 tgcttttact attcctctta ttagtataat ctcagtaaga atgcgtataa aaatgaaaat
    15301 tacaaccgat tttgtaagtg ctgacgcctg agggaatagt atgtgcgaga gactaatggc
    15361 tcgagccata cccctaggca agcatgcacg tacaaaatcg taagataaaa aaataagcat
    15421 atcactgtaa actttaaaaa atcagtttag tgatatgctt atttatttcg agttaggatt
    15481 tatgtcccaa gctcatcaag cacaatcggc cactagttta tttctctatc ttatatgttc
    15541 tgatatggtc ttctatactg tataagtata cttttgaata tggatcttgt gtcaattcac
    15601 gttcgaaatc aaattcttga ttatcaaatc tgttaaagaa tgtttcgtat tcttcgactg
    15661 ataattgctc tctagattct agcatattta agtgtttctc tttatctaat gctttgtcat
    15721 atcctttaac gattgaacca ctaaagattt ctcctactgc tcctgaacca taactaaata
    15781 gacatacttt ctcttctggt tggaatgtgt ggttctgtaa taacgaaatt aaacttaagt
    15841 ataatgatcc tgtataaatg ttaccaacat ctctattcca taatacggtt ctgttgcaaa
    15901 gttgaattta tagtataatt ttaacaaaaa ggagtcttct gtatgaacta tttcagatat
    15961 aaacaattta acaaggatgt tatcactgta gccgttggct actatctaag atatacattg
    16021 agttatcgtg atatatctga aatattaagg gaacgtggtg taaacgttca tcattcaacg
    16081 gtctaccgtt gggttcaaga atatgcccca atttt
//
LOCUS       DAKTIP010000040        16072 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAKTIP010000040 DAKTIP010000000
VERSION     DAKTIP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 16072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16072
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.040"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            65..1633
                     /locus_tag="O0305_001015"
     CDS             65..1633
                     /locus_tag="O0305_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0274166.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGGIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            1775..2533
                     /locus_tag="O0305_001016"
     CDS             1775..2533
                     /locus_tag="O0305_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0274167.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDEK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            2727..3236
                     /locus_tag="O0305_001017"
     CDS             2727..3236
                     /locus_tag="O0305_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCY0274168.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIK
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(3349..4539)
                     /locus_tag="O0305_001018"
     CDS             complement(3349..4539)
                     /locus_tag="O0305_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274169.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNR
                     EKAK"
     gene            complement(4517..5692)
                     /locus_tag="O0305_001019"
     CDS             complement(4517..5692)
                     /locus_tag="O0305_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCY0274170.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            6124..7605
                     /locus_tag="O0305_001020"
     CDS             6124..7605
                     /locus_tag="O0305_001020"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCY0274171.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVD"
     gene            7589..7849
                     /locus_tag="O0305_001021"
     CDS             7589..7849
                     /locus_tag="O0305_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCY0274172.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            7849..9411
                     /locus_tag="O0305_001022"
     CDS             7849..9411
                     /locus_tag="O0305_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCY0274173.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            9713..10516
                     /locus_tag="O0305_001023"
     CDS             9713..10516
                     /locus_tag="O0305_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCY0274174.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            10735..13059
                     /locus_tag="O0305_001024"
     CDS             10735..13059
                     /locus_tag="O0305_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0274175.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTVKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            13076..14434
                     /locus_tag="O0305_001025"
     CDS             13076..14434
                     /locus_tag="O0305_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0274176.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMILMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            14573..>16072
                     /locus_tag="O0305_001026"
     CDS             14573..>16072
                     /locus_tag="O0305_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0274177.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFIV"
BASE COUNT         5617 a         2221 c         3013 g         5221 t
ORIGIN      
        1 tatattgaag tgtctcgtgc ttaatcgtat aatattttta tgtttaaaca atagggggaa
       61 tcttatgatt gaaaaattag taaccttttt aaatgaggtt gtttggagta agccattagt
      121 ttatggtttg ctaattactg gtgtgctatt tacattgcgt atgcgatttt ttcaagttag
      181 acattttaaa gaaatgattc gattaatgtt tcaaggagag aagtctccta atggtatttc
      241 aagttttcaa gcgatagcca tgtctttagc aggcagggtt ggtacaggta atattgtcgg
      301 tgtatctact gcaatattta taggaggacc tggtgcagta ttttggatgt ggattactgc
      361 gtttttaggt gcaagtagtg cttttattga atctacactt ggtcaaatat tcaagagagt
      421 tgaaaataat gaataccgtg gtggaccagc gtattatatt gaatatggta ttggtggtaa
      481 atttggtaaa atttacggaa ttatctttgc tattgttacg attatctcag taggtctatt
      541 gcttcctggt gtgcaatcta acgctatagc aagttctatg cataatgcga ttcatgttcc
      601 acaatggtta atgggtggta ttgttgtagt tattttggga ttaattattt ttggtggtgt
      661 acgtagtatt gccaatgttg caacagccgt tgtaccattt atggcaatta tttacatact
      721 gatggctgtc attatcattt gtatcaatat acaagaagtg ccagcgttat ttgcattaat
      781 tttcaaatca gcatttggat tacaatctgc ttttggtggt atcgttggcg caatgataga
      841 gattggtgtt aaacgtggat tatattcaaa tgaggctggt caaggtacag gtccacacgc
      901 agcagcggca gcagaagtat cacatccaag taaacaaggt ctagtacaag cattttcagt
      961 ttatattgat acattatttg tatgtactgc aactgctctg attatactta tttctggtac
     1021 atataatgtg actgatggta cggttaatgc gaatggcaca ccgcatttaa ttaaagatgg
     1081 cggtatttat gttgaaaatg caacaggtaa agattattca ggtactgcga tgtatgcaca
     1141 agccggcatt gataaagcgt tccatggcag tggttatcaa tttgatccta ctttctctgg
     1201 cgtaggttcg tactttattg catttgcttt attcttcttt gcatttacta caattttgtc
     1261 gtactactac attacagaaa caaatgttgc ttatttaacg cgtaatcaaa ataatcaagt
     1321 ttcatcgata tttattaata ttgctcgtgt gattattttg ttcgctacat tttacggtgc
     1381 agttaaaaca gctgatgtag catgggcatt cggtgattta ggtgtaggtc taatggcttg
     1441 gttaaatatc attgcgattt ggattttaca taagcctgcc gtaaatgctt taaaagatta
     1501 tgaaattcaa aagaaacgtt taggcaacgg ttataatgca gtttatcaac ctgatccgaa
     1561 taaattacct aatgctgtct tttggttgaa gacgtatcca gaacgtttaa aacaagcacg
     1621 tgccaaaaag taatctactt ttgtttatag tatatgtagt gatcatttga taaaaaagaa
     1681 aagtattgag aattttaggt gctcagaaat ttgaatttta aaaatatagt gtctcttggt
     1741 acaataacaa tacaactact aggggcactt ttttatgtca gaatttaaaa ctggtaagat
     1801 taataaacat gttttatata gtaatatttt aaatagagat gtcacgttaa gtatttattt
     1861 accagaatct tataatcaac ttgttaaata taatgtcatt ctttgctttg acggattaga
     1921 ttttttacgt ttcgggagaa tacaacgtac atatgaatcg ttaatcaaag aagcgcgtat
     1981 tgatgatgcg atcattgttg gattccatta tgaagacgtt gataagcgta gagaggaatt
     2041 tcatccacaa ggaagtcgtt ctcatttaac tattcaatca gtcggtaaag aaatattgcc
     2101 atttattgac tcgacgtttt ctacactgaa agtaggtaat gcaaggttat tagtagggga
     2161 tagtttagcg ggtagtattg ccttattaac ggcgttgacc tatccaacga tttttagtcg
     2221 tgtagcaatg ttaagtccac attcagatga aaaagtatta gataagctaa atcaatgtgc
     2281 aaataaagaa caattgacaa tttggcatgt cattggtcta gatgaaaaag attttacttt
     2341 accaacaaat ggtaagcgtg ccgatttctt aacaccgaat agagaattag ctgaacaaat
     2401 taagaaatat aatataactt attattacga tgaatttgat ggtggtcacc aatggaaaga
     2461 ttggaaacca ttgctgtcag atatattatt gtatttttta agtaaaaaca cagatgatca
     2521 actttatgaa taatttacat tagtagattt agtatgaatt gtcttcatat agtctggtct
     2581 ataatataat ttataaaaga ttttactgtt taatttaatt taaatttgac gaaattgcaa
     2641 aagatgtata atgaattatt tttaatgtaa cggttttcaa agaaatttga tataatagca
     2701 ataggttaaa caaaggagga attcagatga ttttaggatt agcattaatt ccatcaaagt
     2761 catttcaaga agcggtggat tcttaccgta aaagatatga taaacagtat tcacgaatta
     2821 aaccacatgt gacaattaaa gcgccatttg aaattaaaga tggtgattta gattctgtca
     2881 ttgaacaggt tagagctcgt attaatggta taccagcagt agaagttcat gctacaaaag
     2941 cttctagctt caaaccaacg aacaatgtga tttactttaa agttgcgaag acggacgact
     3001 tagaagaatt gtttaatcgc tttaatggag aagatttcta tggagaagct gaacatgttt
     3061 ttgtgccaca ctttacaata gcacaaggac tatctagcca agaattcgaa gatatttttg
     3121 gtcaagtagc attagctggg gtagaccata aagaaattat cgatgaatta actttgttac
     3181 gttttgacga tgacgaagat aaatggaaag ttattgaaac gtttaaatta gcttaagtaa
     3241 cataatagta ttgttaatcg tagtatgttt gaattaataa gaaaatggtc atttttattg
     3301 aatgtaataa aaatgaccat tttctttatt ttaaaatacg ttttaacctt acttagcttt
     3361 ttctctattt actataaagt agcttccata aaatacagct aagactaaaa agattaatgc
     3421 cgagaaataa aatgtattgt ttaaattgtt ggtaaattgt gtaattaatc cgccaaataa
     3481 tggccctatc attgagccga atccttggat actattaaaa acaccccaag tttcttcttg
     3541 ttcatctgat ttgataaatc gtgccataaa ggtattccat gctggtaata agatgccata
     3601 cattagaccg atagctaaag cgataatcca caagatgtga atattaacaa tcatagatag
     3661 agtaaaaatt aatatcatgt ataaaataaa tccgcttaga ataacaccat acataaagtt
     3721 tctgctgcgg ttatctatta gtttcgataa aaatagcatc gaaactgcac agccgatacc
     3781 accaataatg attgcaacag tatattcaat tgtgcttacg ttaataacct tagtagcata
     3841 tgttggtaat ataggaacta gggcagcaat tgcggcacct tgtaaaagaa taccagggaa
     3901 caacaataaa tggcgctttg tcacatcaac aatttgtctc aattgagctt taactggacg
     3961 agtattataa tttgttaact ttacatcgac aaaataatat aatatccatg caattaaaac
     4021 gactaaagac atcatgaagg caaagcgtgt tgggtgcact ttgataagta gattcataaa
     4081 aaccatacct accaataggc ctaacaacca tgaaaaataa acatagccca tttgtttgcc
     4141 acgtttatct tcttcaacac tggataacat aatgacccaa ataggactaa ctgcaatacc
     4201 gagcatcata gcactaaata tgattacaaa aggtgatgct ggaaaccaaa taactaaaaa
     4261 taaacttgta aatgctaaaa taaatccagt cgttaaaacg atttttgtgc cgaatttttt
     4321 cagtaaaaat cctataacaa agtttgtaga tgcatcagca ataaaatgta ttgaaaatgc
     4381 tagagacgtt attgctacag caatggatgt aactgttggc aagaaattaa tatagcttag
     4441 gatatacatg cctctcgcaa attccattaa aaataagata ataagcatta aaatgaaatt
     4501 tttatgatta gcgtaattat ttaacgaaga atcttgcata taaaggaacc tttccataaa
     4561 tctcttgtgg ttgtgatgaa tgaccgatta aatcaagtaa gtctcgacat attgtctgtg
     4621 tagcatactt aattttatct tgttccattg tactaatcat gttagttaat tgctcattac
     4681 cgttagttaa acttgctaca atttttattg cttcttctgg agtatcagcg attttaccaa
     4741 aacctttttc ttcaaagtaa agggcatttt caagctcttg accaggtgca ggatttagga
     4801 aaatcattgg aatacaacgg gcgaaacctt cagttattgt gataccacca ggtttcgtaa
     4861 tcataagttg acttgatgcc atccattcat tcatgtgttt ggtataacct agaatcaata
     4921 cattctcgtt agatttaaac ttagctgtta aagaacgctt tagctctttg ctcttaccac
     4981 aaatcataac tacttgtgca tttgcacttt tcgctaatat atcagtaatc atcgtgtcaa
     5041 aacctttaga tacaccaaat gcaccagctg acattaaaat agtttgctta tctggatcta
     5101 agttgttgtc tattaaccac tgcttttgat taataggcgt ttcaaatttg ttatcaatag
     5161 gaatacctgt cactttaact gttgaaggat caatacctac gtctatgaag tcttgtttcg
     5221 tttcttttgt tgccacataa tatcttgttg aatacggcgt aatccagttt ttatgtaagc
     5281 gatagtctgt catcactgta gcaactggaa tattaatgtt aaattgctca gttagtaccg
     5341 acataactgg tgtaggaaac gttaataata ttaaatctgg cttttctttt atcaataaat
     5401 taattaactt attaagtcca tagtatttgt aaaaacattt gtctagttta tctgggcggc
     5461 tgtaataaaa ccctttgtac atatttctaa aatatttaaa gctattgata taccattttt
     5521 tacaaataga agtcaaaatt ggatgagctt ccataaataa atcgtgctca atgacgctta
     5581 aatggtctag attcatatca ttaagttgat taacgatact ctgtgtaact tgcatatgac
     5641 cgttaccgaa tgagccagta ataatcaata tctttttatt ttgagtaacc attaatagcc
     5701 accctccgtt agtttgaaaa ttttatttaa gtgtaactta ttttacggca ttataaagaa
     5761 ataaagacgc aaagtcgtta catttatagc aattttaatc tatagatgaa ttgatacaaa
     5821 ataaaacgtt attttataaa gcaatttatt gttctatgtt ttatttgtat atttaaaatt
     5881 atccagtata caattatagc atatttttgg aaacaattat gatattatac catgttacaa
     5941 gatggtttta ataatttaag atgagccata attgtaaaac taattcataa taccgtatgt
     6001 tttattttta atagtagaaa ttagaaaatg ctgattagta ggatataaca gtgaaattat
     6061 aaatttatta acatcaacaa aacgtgtata ataaacatat tgtagaaaaa ggagcggttc
     6121 agtttggatg caagtacgtt gtttaagaaa gtaaaagtaa agcgtgtatt gggttcttta
     6181 gaacaacaaa tagatgatat cactactgat tcacgtacag cgagagaagg tagcattttt
     6241 gtcgcttcag ttggatatac tgtagacagt cataagttct gtcaaaatgt agctgatcaa
     6301 gggtgtaagt tggtagtggt caataaagaa caatcattac cagctaacgt aacacaagtg
     6361 gttgtgccgg acacattaag agtagctagt attctagcac acacattata tgattatccg
     6421 agtcatcagt tagtgacatt tggtgtaacg ggtacaaatg gtaaaacttc tattgcgacg
     6481 atgattcatt taattcaaag aaagttacaa aaaaatagtg catatttagg aactaatggt
     6541 ttccaaatta atgaaacaaa gacaaaaggt gcaaatacga caccagaaac agtttcttta
     6601 actaagaaaa ttaaagaagc agttgatgca ggcgctgaat ctatgacatt agaagtatca
     6661 agccatggct tagtattagg acgactgcga ggcgttgaat ttgacgttgc aatattttca
     6721 aatttaacac aagaccattt agattttcat ggcacaatgg aagcatacgg acacgcgaag
     6781 tctttattgt ttagtcaatt aggtgaagat ttgtcgaaag aaaagtatgt cgtgttaaac
     6841 aatgacgatt cattttctga gtatttaaga acagtgacgc cttatgaagt atttagttat
     6901 ggaattgatg aggaagccca atttatggct aaaaatattc aagaatcttt acaaggtgtc
     6961 agctttgatt ttgtaacgcc ttttggaact tacccagtaa aatcgcctta tgttggtaag
     7021 tttaatattt ctaatattat ggcggcaatg attgcggtgt ggagtaaagg tacatcttta
     7081 gaaacgatta ttaaagctgt tgaaaattta gaacctgttg aagggcgatt agaagtttta
     7141 gatccttcgt tacctattga tttaattatc gattatgcac atacagctga tggtatgaac
     7201 aaattaatcg atgcagtaca gccttttgta aagcaaaagt tgatattttt agttggtatg
     7261 gcaggcgaac gtgatttaac taaaacgcct gaaatggggc gagttgcctg tcgtgcagat
     7321 tatgtcattt tcacaccgga taatccggca aatgatgacc cgaaaatgtt aacggcagaa
     7381 ttagccaaag gtgcaacaca tcaaaactat attgaatttg atgatcgtgc agaagggata
     7441 aaacatgcaa ttgacatagc tgagcctggg gatactgtcg ttttagcatc aaaaggaaga
     7501 gaaccatatc aaatcatgcc agggcatatt aaggtgccac atcgagatga tttaattggc
     7561 cttgaagcag cttacaaaaa gttcggtggt ggccctgttg attaataaaa gatttattga
     7621 tgaaggtaaa actattgatg tttatttatt cgaagcatta aataaccaga taatcattgc
     7681 tataccagat tggttttggt catatcagat ggcaatgaca ttagatgaag aaacttgttt
     7741 tgaagcaata ctcatgcaat tgtttgtttt taaagaagag gaagaggcag aatcgattgc
     7801 atcacaacta acagattgga tagaaacata taaaaaggag aaagactaat gaacttaaag
     7861 caagaagttg agtctagaaa gacttttgcg attatttcac atcccgatgc agggaaaaca
     7921 acgttaactg aaaaactatt gtacttcagt ggtgctattc gtgaagcggg tacagttaaa
     7981 gggaagaaga ctggtaaatt tgcgacaagt gactggatga aagttgaaca agagcgtggt
     8041 atttctgtaa ctagttcagt aatgcaattt gattacgatg attataaaat caatatctta
     8101 gatacaccag gacatgaaga cttttcagaa gatacgtata gaacattaat ggcagttgac
     8161 agtgctgtca tggtcataga ctgtgcaaaa ggtattgaac cacaaacatt gaagttattt
     8221 aaagtttgta aaatgcgtgg tattccaatc tttacattca ttaataaatt agaccgagta
     8281 ggtaaagaac catttgaatt attagatgaa atcgaagaga cattaaatat tgaaacatac
     8341 cctatgaatt ggccaattgg tatgggacaa agtttctttg gcatcattga tagaaagtct
     8401 aaaacaattg aaccatttag agatgaagaa aatatattac atttgaatga tgattttgag
     8461 ttggaagaag atcatgcaat tacaaatgat agtgattttg aacaagcgat tgaagaatta
     8521 atgttggttg aagaagcggg tgaagccttt gataatgacg cgctgttgag tggagactta
     8581 acacctgtat ttttcggttc agctttagct aactttggtg tacaaaattt cttaaatgca
     8641 tatgttgatt ttgcgccaat gccaaatgcg agacaaacaa aagaagacgt tgaagtaagc
     8701 ccgtttgatg attcattttc aggatttatc tttaaaattc aagccaacat ggaccctaaa
     8761 caccgtgata gaattgcctt tatgcgtgtc gttagtggtg catttgaacg tggtatggat
     8821 gttactttgc aacgtactaa taaaaagcaa aagatcacac gttcaacgtc atttatggca
     8881 gacgataaag aaactgtgaa tcatgctgta gcaggcgata tcattggact atatgatact
     8941 ggtaattatc aaattggaga tactttagtt ggtggaaaac aaacctacag tttccaagat
     9001 ttaccacaat ttacgccaga aatttttatg aaagtttctg ctaaaaacgt catgaaacag
     9061 aagcatttcc ataaaggtat tgaacaatta gtacaagaag gtgcgattca atactataaa
     9121 acattacaca caaaccaaat tattttaggt gctgttggtc agttacaatt tgaagttttc
     9181 gaacatagaa tgaaaaacga atataatgtt gatgttgtta tggagccagt aggccgtaaa
     9241 attgcacgtt ggattgaaaa tgaagaccaa attacagata agatgaacac atcaagatcg
     9301 attttagtga aagatagata tgacgattta gtattcttat ttgaaaatga atttgcaaca
     9361 agatggtttg aagagaaatt ccctgaaatt aaattgtata gtttacttta acagctcaat
     9421 tgtataatcg aatttgttac attaaaaata attgtttcgt tgaagaaaaa taaattgtat
     9481 attttaaaag aaaaaggtat actatgatgt atcaaatgaa taacctatgg cattttgtca
     9541 gaggggagta acttaagaat catgaccgta taaatgattc gacactttat cgtcattacg
     9601 aagatatctt ccggtaaagt gggcaattta aattgcttag tgagaccttt gctatttatt
     9661 tagcataggt ctttttgttt gtacttaact tatttattta aaggagttgt acatgttaat
     9721 ggatccaagt ttgatcttac cttatttatg ggtacttgtc gttttagtat ttttagaagg
     9781 cttattagca gcagataacg cgattgttat ggctgtaatg gttaagcact taccacccga
     9841 acaacgtaaa aaagctttgt tttacggttt gttaggtgca tttgtattta gatttttagc
     9901 attattctta attagtatta tcgcgaactt ttggtttatt caagctgcag gagcggttta
     9961 cttaatttat atgtcaatca aaaatctgtg gcagttcttt aaacacccag aaattgaaag
    10021 tcctgaagct ggagatgatc atcattatga tgaatctggt gaagagatta aagcaagtaa
    10081 caaatcattc tggggaactg tgttgaaaat agaatttgca gatatcgcat ttgccattga
    10141 ttctatgctt gctgctttag ctattgctgt aacacttcct aaagttggta ttcactttgg
    10201 tggtatggac ttaggtcagt tcgtagtcat gttcctaggt ggaatgattg gtgttattct
    10261 aatgcgttat gcagcaacat ggtttgtaga gctattaaac aaatatccag gacttgaagg
    10321 tgcagccttc gcgatcgttg gttgggtagg tgttaaatta gttgtcatgg tattagcgca
    10381 cccagacatc gctgtattgc ctgagcactt cccacatggc gtattatggc aatctatttt
    10441 ctggacagta ctaattggat tagtaattat cggttggtta ggttcagttg ttaaaaataa
    10501 aaaatcgcat aaataattga tgtgaagcgg acaatcttaa tttagtttaa ggttgtcctt
    10561 tttcatttaa ttgagtgatt tatgaaaaat ggattttgaa gaatgtgaat caaaagatgc
    10621 gatatagtat taagaaaatg tgccttttat atttagcatt ttttcaatag aaattatata
    10681 gattttaaag caaattaggt gttaatgtgt cataatgata agtgatttta ttgaatggag
    10741 tggacattag tggatattgg taaaaaacat gtaattccta aaagtcagta ccgacgtaag
    10801 cgtcgtgaat tcttccacaa cgaagacaga gaagaaaatt taaatcaaca tcaagataaa
    10861 caaaatatag ataatacaac atcaaaaaaa gcagataagc aaatacataa agattcaatt
    10921 gataagcacg aacgttttaa aaatagttta tcatcgcatt tagaacagag aaaccgtgat
    10981 gttaatgaga ataaagctga agaaagtaaa agtaatcagg atagtaagtc agcatataac
    11041 agagatcatt atttaacaga cgatgtatct aaaaaacaaa attcattaga ttcagtggac
    11101 caagatacag tgaaatcaaa atattatgag caaaattctg aagcgacttt atcaactaaa
    11161 tcaaccgata aagtagaatc aactgaaatg agaaagctaa gttcagataa aaacaaagtt
    11221 ggtcatgaag agcaacatgt actttctaaa ccttcagaac atgataaaga gactagaatt
    11281 gattctgagt cttcaagaac tgattcagac agctcgatgc agacagagaa aataaaaaaa
    11341 gacagttcag atggaaataa aagtagtaat ctgaaatctg aagtaatatc agacaaatca
    11401 aatacagtac caaaattgtc ggaatctgat gatgaagtaa ataatcagaa gccattaact
    11461 ttaccggaag aacagaaatt gaaaagacag caaagtcaaa atgagcaaac aaaaacctat
    11521 acatatggtg atagcgaaca aaatgacaag tctaatcatg aaaatgattt aagtcatcat
    11581 ataccatcga taagtgatga taaagataac gtcatgagag aaaatcatat tgttgacgat
    11641 aatcctgata atgatatcaa tacaccatca ttatcaaaaa cagatgacga tcgaaaactt
    11701 gatgaaaaaa ttcatgttga agataaacat aaacaaaatg cagactcgtc tgaaacggtg
    11761 ggatatcaaa gtcagtcaac tgcatctcat cgtagcactg aaaaaagaaa tatttctatt
    11821 aatgaccatg ataaattaaa cggtcaaaaa acaaatacaa agacatcggc aaataataat
    11881 caaaaaaagg ctacatcaaa attgaacaaa gggcgcgcta cgaataataa ttatagtgac
    11941 attttgaaaa agttttggat gatgtattgg cctaaattag ttattctaat gggtattatt
    12001 attctaattg ttattttgaa tgccattttt aataatgtga acaaaaatga tcgcatgaat
    12061 gataataatg atgcagatgc tcaaaaatat acgacaacga tgaaaaatgc caataacaca
    12121 gttaaatcgg tcgttacagt tgaaaatgaa acatcaaaag attcatcatt acctaaagat
    12181 aaagcatctc aagacgaagt gggatcaggt gttgtatata aaaaatctgg agatacgtta
    12241 tatattgtta cgaatgcaca cgttgtcggt gataaagaaa atcaaaaaat aactttctcg
    12301 aataataaaa gtgttgttgg gaaagtgctt ggtaaagata aatggtcaga tttagctgtt
    12361 gttaaagcaa cttcttcaga cagttcagtg aaagagatag ctattggaga ttcaaataat
    12421 ttagtgttag gagagccaat attagtcgta ggtaatccac ttggtgtaga ctttaaaggc
    12481 actgtgacag aaggtattat ttcaggtctg aacagaaatg ttcctattga tttcgataaa
    12541 gataataaat atgatatgtt gatgaaagct ttccaaattg atgcatcagt aaatccaggt
    12601 aactcgggtg gtgctgtcgt caatagagaa ggaaaattaa taggtgtagt tgcagctaaa
    12661 attagtatgc caaacgttga aaatatgtca tttgcaatac ctgttaatga agtacaaaag
    12721 attgtaaaag atttagaaac aaaaggtaaa attgactatc ccgatgtagg tgttaaaatg
    12781 aagaatattg ccagtctaaa tagttttgaa agacaagcag ttaaattgcc aggaaaagtt
    12841 aagaacggtg ttgttgtaga tcaagttgac aacaatggtt tagcagatca atctggtctg
    12901 aaaaaaggtg atgtaattac tgaattagat ggcaaacttt tagaagatga tttacgcttt
    12961 aggcagatta tatttagtca taaagatgac ttgaaatcaa ttacagcgaa gatttataga
    13021 gatggtaaag agaaagaaat taatattaaa ctaaaataat gttgaggtga acaacgtgtc
    13081 aatttttagc cagtttttaa aaagatcaag ccctcaacaa ggtattgtat tgtactatat
    13141 cgtcgcaatt gtcattgcat ttttattatt aaacttaccg tatgttcata aaccaggtgt
    13201 agaagtaaat ccaattgaca cattatttgt tgccgtatcc ggaattagtg ttactggatt
    13261 gtctccgata agtattgtcg atacctattc tacatttgga caattaatta tcctcgtgat
    13321 attaaatatt ggtggaattg gcgtcatggc aattggtacg atgttatggg tggtactagg
    13381 taaacatatt ggaattagag aacgtcagtt aattatgtta gataataaca aaaacacaat
    13441 gagtggtacc gtcaaattga ttattgatat tgtaaaatca atatttgtaa tcgaactcgt
    13501 aggagccatg ttattagcat tttactttta tcgagataat ccagatttaa aatatgcaat
    13561 catgcaaggt gtttttgttt ctatttctgc cactaccaat ggtggattag atattacagg
    13621 taagtcatta attccttatg cacatgatta ttttgtacaa gcgatagtta tatttttaat
    13681 aattttagga tcaatcggct tcccagtatt attagaagtt aaagcttata ttcaaaatag
    13741 ggttactaat tttagatttt cattatttac taaaattacg acatcaacat atttattcct
    13801 atttattgtt ggggtattag ccattctatt atttgaacat aaccatgcgt tcaaaggttt
    13861 aagttggcat caatcgttat tctattcgct gtttcaatca gcgactacaa gaagtgcggg
    13921 tcttcaaaca attgatgtga caacactaag tgaccccact aatattatca tgggtatttt
    13981 aatgtttata ggatcttcgc caagttcggt tggtggcggt attcgtacaa caactttcgc
    14041 tattttaatt ttgtttttaa ttaactttag taataatgcc gataaaacat ccattaaagt
    14101 ttacaataga gaagtacaca ttatggatat tcaacgttca tttgcagtat ttacaatggc
    14161 gacaatttta acatttttag gaatgctaat tatatcagct actgaaaatg gtaagcttac
    14221 atttttacaa gtattttttg aagtcatgtc tgcatttgga acttgtggac tatcgcttgg
    14281 tgtcacaagt gatattagtg atatttctaa ggtcgtacta atgatactca tgtttatagg
    14341 acgtgttggc ttaatatcat ttatcattat gatagcagga cgtcgagaac cagataaatt
    14401 ccattatcca aaagaacgta ttcaaatagg ataatataat agcaatctaa gtttagttaa
    14461 tgtagatttt aactggaact tagattgctt ttttagtttg tatttttaac ttattttata
    14521 agacgattgg tttcgaaaat ggtaaactag taacaatgag aggtgtaaca tgatggaaaa
    14581 aaatgaaaac attaatgtag agattttaac tacgtcagat atgcatagtc atttcttaaa
    14641 tggtgattat ggttcaaata tttatagagc tggtacttat gttaaccaag taagagcaca
    14701 aaatcatcgc gtcattttat tagatagtgg cggaagttta gctggctcgt tagcggccta
    14761 ttattatgct attgttgcac cttataaacg acatccaatg ataaagttaa tgaacagaat
    14821 gcattatgat gctagcggtg tgagtccaag tgaattcaag tttggtttat catttttaac
    14881 tcgttcaatt gctttggcac gttttccatg gttatcagca aatattgaat acaatgttac
    14941 taaggagcct tatttttcaa ctccatattg tattaaacat tttggtgact taaaaattgc
    15001 tatcgtaggc gtcacagcag atggtttaat ggaaaatgag tattctgaaa tggagcaaga
    15061 tgtatctatt gaaaagacat tagtggcatc aaaacgttgg attagatata tccatgaagt
    15121 tgaagagcca gattttttga ttgtaattta tcatggtgga ttgaataaaa ttagtaatag
    15181 tacgaaaaat aaaaaggcaa gttcgaatga agctgaaaaa ttaatggaag aactcggtgt
    15241 tatagattta atgattacag ctcatcagca tcaaacaata gtaggtcaag atcatgaaac
    15301 gtattatgtt caggctggtc aagatgccaa agagcttgta catctttcga ttaattttaa
    15361 aaagagaaca acaacttatg atgttgaaag cattgattct aaagtgattg acttaaatga
    15421 gtatgaagag gatcaagaat tattagattt aacattctat gatagaaaag cagtggctta
    15481 ttggtcacag gaaatcataa gtgataaagg tttgatgtta tcagtaaatg ggttacaaga
    15541 tttagtctgt caaacacatc cattttcgca attattacat gatgcaattc accttgcatt
    15601 tgataatgat ataacatgtg tccacgtgcc tatgaacgga gagaaggggt tgagtggaca
    15661 gattcgaaat gaagatttgt atcatgcata cccatatcca gataagccaa tggatatgac
    15721 aattagtggt caaaatatca aagatatatt ggagtatagt tattcacatt tagattttgt
    15781 taacgagcaa ttaagcttaa caattattga tgaaacgtta tgtacaatgt ggcaaggatt
    15841 caattatgag attgatatga atcaagaacc tgggcaacga gtaatgttag atcaaattga
    15901 tttgactaag agttatagag ttacaatgac tgactattgt tatcgtaact acaagaatta
    15961 tttaaaaaat gctattatac atgaatcata cgatgaaaca atgagtacat taattgcaga
    16021 gaagttaaga gatccgaatt atcgtatttc atgtagtgat aattttatag tt
//
LOCUS       DAKTIP010000041        15937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAKTIP010000041 DAKTIP010000000
VERSION     DAKTIP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.041"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(168..1460)
                     /gene="asnS"
                     /locus_tag="O0305_001027"
     CDS             complement(168..1460)
                     /gene="asnS"
                     /locus_tag="O0305_001027"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCY0274178.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(1782..4475)
                     /locus_tag="O0305_001028"
     CDS             complement(1782..4475)
                     /locus_tag="O0305_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase DinG"
                     /protein_id="HCY0274179.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(4499..5470)
                     /locus_tag="O0305_001029"
     CDS             complement(4499..5470)
                     /locus_tag="O0305_001029"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCY0274180.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLEILLQEIEKRYNQFLTLPFSEIREEYTAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(5457..6659)
                     /locus_tag="O0305_001030"
     CDS             complement(5457..6659)
                     /locus_tag="O0305_001030"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCY0274181.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(6664..7806)
                     /gene="bshA"
                     /locus_tag="O0305_001031"
     CDS             complement(6664..7806)
                     /gene="bshA"
                     /locus_tag="O0305_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCY0274182.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(8047..8364)
                     /locus_tag="O0305_001032"
     CDS             complement(8047..8364)
                     /locus_tag="O0305_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCY0274183.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(8701..9381)
                     /locus_tag="O0305_001033"
     CDS             complement(8701..9381)
                     /locus_tag="O0305_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCY0274184.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(9453..10040)
                     /locus_tag="O0305_001034"
     CDS             complement(9453..10040)
                     /locus_tag="O0305_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCY0274185.1"
                     /translation="MTIKAFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(10030..10605)
                     /locus_tag="O0305_001035"
     CDS             complement(10030..10605)
                     /locus_tag="O0305_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCY0274186.1"
                     /translation="MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDN"
     gene            complement(10619..11863)
                     /locus_tag="O0305_001036"
     CDS             complement(10619..11863)
                     /locus_tag="O0305_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274187.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(11870..13168)
                     /gene="aroA"
                     /locus_tag="O0305_001037"
     CDS             complement(11870..13168)
                     /gene="aroA"
                     /locus_tag="O0305_001037"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCY0274188.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(13178..14242)
                     /locus_tag="O0305_001038"
     CDS             complement(13178..14242)
                     /locus_tag="O0305_001038"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCY0274189.1"
                     /translation="MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFD
                     SKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(14268..15434)
                     /gene="aroC"
                     /locus_tag="O0305_001039"
     CDS             complement(14268..15434)
                     /gene="aroC"
                     /locus_tag="O0305_001039"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCY0274190.1"
                     /translation="MRYLTSGESHGPQLTVIVEGVPANIEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         5095 a         2918 c         2132 g         5792 t
ORIGIN      
        1 atgaactatt tattttccaa atatagttac taaacaaaag tttgttcatg attaatataa
       61 gccgttatca atcttcattt tccaagaata aatcgcccag ctattaatat aggctggacg
      121 attcttttta ttatcataat atatatttta agtaacgacc ctaaaactta tggatataaa
      181 cggtttaata atcttgggaa tggcgctgtt tcacgaacgt gttcaacacc agaaatccat
      241 gctactgtac gctctaaacc taaaccaaat ccacagtgtg gcacactacc ataacgacgt
      301 aagtctaagt agtaactata tgcttcttcg tctaatccat gttctttaac gcgttgttct
      361 aacaattcta agtcatccac acgttcagat ccaccaataa tttcaccgta tccttcaggt
      421 gcaattaagt ctgcacataa tacagtttct tcattttcag gatttggttg catatagaaa
      481 ggcttaattt tagttggata attagtaata aacaccggta aatcataatg attagcaatg
      541 gctgtttcat gtggcgcacc aaaatcttcg ccccattcaa tatcatcaaa gccttctgct
      601 tttaagaatt caattgcatc atcatatgaa attctaggga atggtgtcgc aactttttca
      661 agttttgatg tatcacgctc taaaattttc aactctagtt tacaattttc taaaactgat
      721 tttacaacat gtgttacata ttgttcttga atttctaaac tttcagcatg atttgtgaaa
      781 gccatttctc cttcaatcat ccagaactcg atcaagtgtc tacgtgtttt tgatttttca
      841 gctctgaaag ttggaccaaa tgaaaatact tttccgtgtg ccattgctgc agcttctaag
      901 tataactgac cactttgaga taaaaacgca tcttgatcaa agtatttagt atggaataat
      961 tcacttgtac cttctggcgc acttgctgtc aaaattggtg gatcaacctt tgtaaatcca
     1021 tctttgttga aaaattcata cgttgcacga ataacttcat ttctaatttt cattacagca
     1081 tgttgttttt tagaacgtaa ccataaatga cggtgatcca ttaagaattc tgtaccatga
     1141 tttttaggtg taatcggata gtcatgcgct tctgaaataa cttcaattga tttcacttgc
     1201 atttcgtatc ctaagtcaga acgattatct tctgtaattg tgcctgtaac gtatagagat
     1261 gattcttgag taatttcttt cgcaagtttg aatacctctt catcaacttc tgatttaact
     1321 actacgcctt gcataaagcc tgttccatca cgtaattgta aaaaggcgat tttaccactt
     1381 gaacgtttat ttgttaacca agcaccaatt gtaacgtctt ggtttaaatg atctttcgct
     1441 tgtttaatcg ttgttttcat aaccattctc ctatttattt tttcgttata caatactcat
     1501 tcattttaac aaaatccgct ttcaagttct agaactagac taaaagataa cgtgtaaatg
     1561 gtaatgattt acgcacagat ttgaacatta atttatatta agacaataca atcatcagtt
     1621 tcagacaaaa tatataataa agcctcaata ttattagtat gaaggtcgtt ttttgtgcta
     1681 acattcaagt ttaattataa aactcaaatt ttgacgacca aacaaaaatt cttgtaacag
     1741 aacatagcac atgtcatgtc acaagaattt tactttgtat ttcacttttt ctttttttga
     1801 atttgtctta ataattttcc aaactgttga atgtcgcctt ttttctgacg ataattttca
     1861 agtgtttgtt caaaaaagtt tttataatta ctgtttatga gtcgatcatc aaatgaaact
     1921 attatgccgc gatcattttc atttctaatt aatcttccaa gtccttgtct aaaacgtgta
     1981 actgcatcag gtaatacata ttccttgaaa gttgaagtga attcagaatc cataagccaa
     2041 tattttgcat tatgcttgtt cataaacggt aacttcgcta tcatcacaca tttaatacca
     2101 tttgcttgaa aatcaaaacc ttcaaaaaat gttgacgtac caagcagtat ggccttatca
     2161 aaattattaa actgttgtac tattttataa ttttggttct gctgttgtgt taatacaaca
     2221 taatcttcaa attctggcaa ttcatttagc atatcttgta ccatatgcat cattttataa
     2281 ctcgtaaata agactaaaca ttttgatgac gttatagtcg tatattcaat aatatagctt
     2341 acaatcgatg ctacatactc atctatattt ttatattgat aagatgctac atcactcggt
     2401 ataaatacac ttgtattttt tgcactttgt aacgacgtgt taacttcaaa tgtattaaag
     2461 tgaacatctt tgttgaataa ctgtttaaaa gcttcaaacg aatgattaaa ttttaaagta
     2521 ccagatataa aaatgagcga tttaaatttt tccaatactt gtttcgttaa tacatctttt
     2581 acagcgtaat ctttcacata taaacgtatt gttgattttt gagataaatt tttaatcgaa
     2641 atgaaactag tatgtccagc ttttaaactt tgttcgatat ttttaaattt atcttttaaa
     2701 tacaacaact gtttccgtaa tgatttaact gttttatgac taatgccatt gaatatttct
     2761 agcgttttat ttaacttatc gataatcgca tgtaaatcct tcaaaatgtc ttttgtttca
     2821 aaagtaaata cattatggaa gcgatgaata tcatcatcat aaacatcaga atcattgata
     2881 atcgtaaata tcgttgagaa caattgctca tttaactcat gaatctcatt catactagcc
     2941 ttcaagccaa aaatatcaat tggtgcaata tctaattttt ccaaaattcg ctgcttttcc
     3001 agttgatcaa ttgcctttaa caatttttca ttttcgtttt taccaatcaa accaagctga
     3061 tatttaatat cagcataact caactcattt gtcacttgat ttaaggcata gtctggtaag
     3121 cgatgtgctt catccactat acaatcatca aacaattgat atattgaatt ttcaacatca
     3181 gaatgaatta aatgtgcatg atttgtaata ccaatttgaa tgttctgtgc atttcgctta
     3241 ataaaattat aataatgaac atcgtgacgt gccggtacat atgtttcaat tttctggtca
     3301 aaatacatct tttgaccacc ttttaaattt aattcctgta tatctccgga cggcgtctct
     3361 gtaatccaaa tcagcaattg cattttcaag atattcactt cgtaattact tgtgtcatct
     3421 tttaaaattt gactaataag ccccaatgaa atgtaatcac ttttactttt aatcaatagt
     3481 gcattaattt taaaattcaa cgcttcattc attgctggaa tatctttttc taacaattga
     3541 ctttgcagta atttagtatt ggtagaaatc atgacatgct tcccagtttc aatattatac
     3601 atcaaggccg caagtaaata tgctaatgat ttaccactgc ctagtgatgc ttcaatcatt
     3661 gctttttcac tatgcatgag ctgatctaat atagtttccg ctaaatataa ttgttgcggt
     3721 cgatatgtta agccaagttg atctacagct ttgctatata aagacttcaa gctgccatta
     3781 taatttgttg tcggcttttt aaaatcaact tgcttacgat agataatctg ttcgaacttt
     3841 tcgtacgatt tatccaatgg ctttgcatca tattgcctaa ccatctcaaa gaaaatatca
     3901 tacaaatcgt atttcaactg tttacttaaa taatataatt gcttcaaagt atctaacggt
     3961 aacttttcaa atttttcaaa agctaatatc atcaatttag cagtagtagc ggcatcttcg
     4021 tcagctcgat gggcatttgc taaggtaata ccatgtgcct ctgctaattc acttaattga
     4081 tagcttttat ctgtaggaaa agctatttta aagatttcta gtgtatctat aacttttttg
     4141 ggacgatatt gaatattaca atctttaaat gcctttttaa taaaattcaa atcaaaatct
     4201 acattatgag ctacaaaaat gcaatctttt atcttatcgt agatttcttg tgcaacttga
     4261 ttaaaatatg gcgcttgttg tagcatattt tcttcaatgg atgttaacgc ttgaatgaac
     4321 ggcggaatct ctaaatttgt tctaatcata gaatgatatg tatcaataat ttggttattg
     4381 cgcacaaacg ttataccaat ttgaatgata tcgtcaaaat ctaattggtt gcctgttgtt
     4441 tccaaatcca caacggcata ggttgccata cccatagcta tctctccttg ctttagtgtt
     4501 aaaaatctat atctgcacta attaaacggt gtgattcacc cgcttcatct ctaacaatta
     4561 gatagccatc gtaatctaaa tcaattgctt gtcctttaaa ctgtttatca ttttctgtaa
     4621 atagcaacgt tctattccaa atattagaag ctgcagtata ttcttcacga atttcagaaa
     4681 aaggtaacgt taaaaattga ttatatcttt tttcaatttc ttgaagtaat atctctaaaa
     4741 attgatatct atctaattta tttttatcat gtaattgtat acttgttgct ctatgtctaa
     4801 tactttcatc aaagttttct agttgttgcg tcaaattaat acctatacca catattattg
     4861 cttctatacc atcattatta gcaaccattt cagttaagaa accacacact ttaccattat
     4921 caatatatat atcattcggc catttcactt tgacttcatc ttgactaaaa tgttgaatcg
     4981 catctcttat ccctaatgca ataaataaat taaatttaga tatcattgag aatgcaacgt
     5041 taggtcttaa cacgacagac atccaaagtc cttgcccttt tgaagaactc caatgtctat
     5101 taaatcgccc acgacctttc gtttgttcat cactcaagat aaaaaatgaa gattgatttc
     5161 caacaagtga ctttttcgca gcaagttgtg tagaatctat tgaatcgtat acttcactaa
     5221 aatcaaacaa agcagaactt tttgtatatt ggtctattat accttgatac caaatatctg
     5281 ggagctgttg taataaatgc cctttatgat ttactgaatc tattttacat ccctctaact
     5341 ttaattggtc aatcactttt tttactgcag tgcgtgaaat attaagtgat tccgcaatgc
     5401 tttgtccaga tatataattc ggtttatttt tatagagtaa ttgaagtaca tcttgactat
     5461 attttgacat gattatccac ccatttcaaa atttcagttt cttcgttgct tactttacct
     5521 gttacaatcg ctatctcaat ttgtcttagc acatctttta accacggacc acttttggca
     5581 tttaaatgtg ccataagtac accgccatta accatcatgt cttttctatt atgcataggt
     5641 aaacgatgta atgtttcatc aatcgtttga aggttaacga ttaatggttc atgtccttga
     5701 atatcatttg cctttaacac gtctgctgca accattacat ttttaatgag atgcgtatca
     5761 taatcataaa caaacatttt taattgttct tttgtaataa tacttggtaa tgcattcata
     5821 atttgaatat attgattgat atcttttact tgtcggttac ttagctttaa aggcttcaat
     5881 gagtaattaa tatcaaattt aactgatact atagcaatca acaattctaa atcaattgct
     5941 tcagttacat taatttgatt catatcaaga tgttcgaaat acggcatata attaaatgct
     6001 ttcagcgatt ttaaatgatt aaaactcttt tcaacattaa tacctcgcat taatttagtt
     6061 agttcaatca ctatacgctc aattgataaa aatttaatat ctgccatttg tatacgcatc
     6121 gcttcgaatg tttccgttgc aatatcaaat gataattgtg actggaacct taaacatcga
     6181 atcatacgta aagcatcttc ttggaatcgt tcctcagcta tacctacagt tcttattatt
     6241 cgattattaa tatcttgttg accatcaaaa taatcataca atttgtatgc tgtatccatt
     6301 gctatcgcat tcatcgtgaa atctcgtcgt tgcaaatctt cgtataaatc acgaacaaat
     6361 gtaacaccac ttggtctacg gtgatcgaca taatcttctt cagcccggaa tgttgtcact
     6421 tcataatttt catcattaaa aactacattt atcgtgccat gttctttacc tacaggtatc
     6481 gtatgactaa agatagattc tatttcatcc ggcgttgcac ttgttgtgat atctatatca
     6541 tgaatatttc ttcccatgac ataatctctt acagagccac ctacataata tgcttcaaaa
     6601 ccattgtctt gaatttgttc taatataggc cttgcctgtt caaataatga tttatccata
     6661 ttattactcg cctttacttt tgttatgctc atttagcatt ttttgataat aatactcata
     6721 ttgatctgta ataagttctg atccaaaacg ttcagcaata tctgctagca tgtttttctg
     6781 aagtttgttg tataacacct tatcttcaag taatcggata gcatagtcac tcgctgaatc
     6841 acaatcaccc acatctacga caaatccagt ttcaccatgt ttaataacct ctttaattcc
     6901 accggcattt gaaccaattg gaacgacgcc tgttttcata gcctcaagta aagttagtcc
     6961 aaagctttct ttttcactta ataataatac taagtcagat aattggtaaa attcacttac
     7021 gcaatcttgt ttccctaaaa ataaaacatc ctcttctacg tttaactctt tcgtcaattg
     7081 acgcattggc actaattcag gaccatctcc aagtaaaatt aatttactag gtatcttttc
     7141 acgtactttt gcaaatgttt ctataatagt atctatgcgt tttacttgtc taaaattcga
     7201 tacatgtatt aacacttttt catctggtgc tataccaaat tgtgatttta atgctgtgtt
     7261 atgtttagtt ggaaactcat tttcacgtac aaaattataa atcggtataa tttctttgtt
     7321 agtttcaata atttcatgtg tttcttgtgc taaagattta ctcacacttg tcacaatatc
     7381 acttttttca atgccaaatt taattgcacc ttggagtgaa tgatcatagc ccaaaacagt
     7441 aatatcagta ccgtgtagcg ttgtcataat ttttatatct ttacctgaca tctcacgagc
     7501 taaaatccca caaattgcat gaggtacagc atagtgcata tgcaacaaat caagatcata
     7561 ttctttaata acttcagcga ttttagtact taacgtaata tcatacggtg gatactgaaa
     7621 tactgcatat tgattcactt caacttgatg aaaaatcata ttcggtaatg gttttcttat
     7681 tctaaacggg atatttgaag tgataaaatg tacttcgtga cctcgctctg ctaatttaat
     7741 tcctaattct gtggcaataa ttccagaacc acccatggac gggtaacatg ttatacctat
     7801 cttcattcgc ttgcccatcc tttctttcta tttctctcta ttattctcga tgcgtagata
     7861 attgtttaaa tttaagttta tagtaatgtt gagtttataa tttcatatat ctaaaaacag
     7921 gtgttgtata tataatcatt catctagtta tacttacttt aaaaataata taatttcatg
     7981 cgatgcaatt cattgatgga tgtttttaat cttaatcaaa tccaaataaa gcatatattt
     8041 ttaaattcac tttctttcga atcgattttt atctcttgta ttaaactttt ccattgtttc
     8101 attaaagctc tctgtcatat ctattcccat tgaattcgct aaacataaca acacaaataa
     8161 attatcacct aattctgctt taatcgtatt tgcttcctct gaatctttct tctttttttc
     8221 accataggta tgatttattt cacgtgcaag ttcgcccact tcttcagtca atctagctaa
     8281 gttagctaat ggtgaaaaat atcctgtttt aaattgtcca atatattcat caacttcacg
     8341 ttgcatttct accattgatt tcatttctac gttctcctta tattgcattt ctaatatagt
     8401 atatatcaat ttgaagtctc atgcatgttt agtgcaatct actgactcta gctaaactag
     8461 atgacaagtt gtttatttta gaagtttaat aattcagttt atataaatgt aatgcattcc
     8521 taactaaatt aaatcaattg aaattgggat tataacttta tgatacgtac cactacaata
     8581 aaataatata gtgaataatc taccattaga aaaataagca caaaaaaact agcaaccaca
     8641 caaaaatgtg attagctagt taataagtgt ctaatttaag ttaattgtta atctataaga
     8701 ttaatcactt gaacgcgcaa tcaaaataat acgtacaagc tctgctacag cgactgcagt
     8761 tgctgcaaca taagtcattg ctgctgcaga taatacttta cgcgcatgct tgtattcttt
     8821 ttcatttaca atgttcaatg ccgtaatttg tttcatcgct cttgaactcg catcaaactc
     8881 aactggtaac gtaacaattg agaataatac cgctaatgac attaaaccag caccaatcca
     8941 taaagcagtt gaaccaaatg cactacctat cgctgttaag ataataccta acatgatgat
     9001 catataactt aatgaactcc ctaggtttgc aacaggtact aatgctgctc tgaatcttaa
     9061 gaaccaatat ccttggtgat cttgaatggc atgaccaact tcgtgggctg caattgcagt
     9121 tccagcaact gatggtctgt catagtttgc aggagatagt gaaacaactt tctttttagg
     9181 atcgtaatga tctgttaaga atccttcacc tttaacaact tcgacatcat aaataccgtt
     9241 tgcatgtaaa atttctaatg caacttcacg acccgtttta ccactagttg atctaacttg
     9301 tgaatatttc tcatagttag atttaacttt gtgttgtgcc cataaaggaa gcaccattaa
     9361 tattacgaaa taaattatca tagtaaaaat tgaagacaat aaactcactc tcctttataa
     9421 atattttact gtcatttgcc gtttttatca aatcatttac actttaataa tttgtttaat
     9481 tcaatataaa gcaaaagtcc aaaaacactt agacaacatg ataatacacc aatttgccac
     9541 acatgtgtag ttataaaatc ataatatgga aattgaaggt gaaaatagtc aatataatca
     9601 ttcaaaaaca cccaaatcat cgctacactg attccaatca tagaacgttt aaacctagga
     9661 tagaagtaaa ttgcctgaac agccattata ctgtgggaaa acattaatac caaaccattt
     9721 actgtaatat caccttgttc aataataaat aatatattca ttataactgc ccaaatccca
     9781 tatttgaata atgttacaaa tgccagtgca tcgataatac tattttgttt ttgaattaat
     9841 atcaatgaga tagaaataac taagtataat attgcagttg ggctatctgg aacaaaaatc
     9901 ttaaaatgcc agggcgtatg acttaattgt tcaccatacc atatataacc ataaatcatc
     9961 cctaatatat tacaaatgag tagcatcatt aaccaagaac gttgataaag tgtatattgc
    10021 caaaatgctt taattgtcat ctgctaagtc ctcaaattga ttatgtttat ttactagctt
    10081 gagtgtattt aaaatttgcg ttagttgata aaaacgttgc ttttcattca tctgtaaact
    10141 taaatcaata ttgtgtaaca agtaatctat taataacgca tgtttatgcc gatctatagc
    10201 catactattt aagtcatgaa gataagtttg ataactgggc gattccgtta gttgagcaac
    10261 tattaaatca tctagccttt gctcacgctt tgacacgttt gcgaagtgaa tttgaatatc
    10321 aaaagcacag ttatgattag cgatataatc aaatatttca tttgtattca ttaactttat
    10381 attacgctta gtaaattgaa ttgcagaagc gtgacttccc acttctgcaa tttctaatgt
    10441 ttcatgatga ttaatttttg tatctacaaa atgaatgttt gccaatttcg cctcattcac
    10501 ttttatatag ttaagcaccc aaactgcaat acgcgactta aatcgatatt gaaaaagtaa
    10561 atattcaata aaactttctt taatttgatt gaatgtctct gacatcaaat accccatttt
    10621 aagattgcaa tcttgataat tcgtcatgcc aattttcgtt acttggctct agttccaaca
    10681 attgatttaa aatagtaatt gcttgttcct tttgaccaat ttcaattaaa tagaaataat
    10741 aatcactcat aaaatcaata tttgttttca tcgttggata tgctaattca aagaaatgtt
    10801 gagcttcttt atctcgctct tcttgaccat aggcgaacgc taaatgccac atgaatgtag
    10861 gatccaaatc ttcttcatct acatatgtta ataattcaat gattgcttca taatcttctt
    10921 cattacgata taaatcgctt aaaatcaata aaggttcttg gtaagcatta tcaacctcta
    10981 atgcttgttt taacaataat acaccttcat tagcatcgcc gtgttctatt tccaaacatc
    11041 cagttgtata cattaactct ttataaaatt gacttagtcg taatccttct ttacccgttt
    11101 caatggcatc tggataattt ttttcatttt catataatga ttgtaaatac aagtagcctt
    11161 gaatataatc aggatcttta gaaagtaatg tagtcattat tttaattgct tcttgagtga
    11221 tgtcattttt atcgtaagaa atggcttttt tgagataatc ttctgaagtc atttcatctt
    11281 cattaatttc atcgtataag cgaatcgcat cactatagtt accactttgt aaactacaat
    11341 ctgccatacg agagaataag tttacaccat taacttgata ttcaccagtt tctaaaacgg
    11401 tttcgtattc agaggtagca cgtaaatatt gaccatcata atataacatt tcagccaatg
    11461 caaaatggat tattggatca tttggctcta gttcaagtgc ttcttgtaat ttatcaatag
    11521 caacttccat catattaatt tgttgatata aatctgcttc taacatcaac ttttcaggtg
    11581 atggttcaac ataacttaaa tattctaacg cttcgtcagt ttgattttca gacattaaac
    11641 cttcaataaa ataaatcagc aattcacttt cgtctggata tttgtgatat aacacgcgga
    11701 atacttccaa accttgtggc attaatccaa aattgtaaag tgtctctcct agaataaata
    11761 atgcgtcatc gttgtcagta gttattgctt cattaacacg agagtctaaa ttttctagtt
    11821 tttgtagatt gatatcgtct attaatttat agatatcttc cattttatat tatccctcat
    11881 tttctaaaag ctttagtttt ggtaaaaatc ctggaaatga tacatttaca gcatcaaatt
    11941 gtttgatttt gacaggctcg cttgaaagta gagaagcaac tgcaagcatc attcctattc
    12001 gatgatcagt taaactatca actgttgcat ttgttttaaa ttctgacgga tgaataatca
    12061 atccatcatt agttggttgt aattcaaacc ctaacaagtt taacatatca gccgttgtat
    12121 caattctatt tgtttctttt acttttaatt cctcggcatc tttaattgta ctcgtgccaa
    12181 ctgcttgtgt acaaagtaat gctattacag gcagttcatc aattgctttt ggaactaatt
    12241 ctccttcgat tgttattggt tgaagcattg gtgtgtattg aatacgaata gaagcagtag
    12301 gttcagcacc agttgtttga ttgaaaagtt ggatattacc gcccattttt tcaacaatat
    12361 caataatacc tgaacgtgtt ggattgattc caacattatg aattgttaca tcacttcctg
    12421 gtgtgataag tgctgcaaca ataaagaacg ctgcagatga aatatcgcca ggaacatgaa
    12481 aatctgcagg tttaatgtat cgaattgctt caggggttgt attaattgat aacccttctg
    12541 cttcaattgg aatattaaaa tgtttgaaca tcgtctcagt atgatttcga cttacatcta
    12601 attctttaat gatggtcggt tccttagaaa acaaacttgc aaataaaatg gcacttttta
    12661 cttgtgcact tgcaacttcc atttgataat ttataccttt tatgacagat ggcttaataa
    12721 ttaatggtgt ataattatct tcaatacctt caatattcgc atccataagt ttcaatggtc
    12781 tcaagacacg atccattggc cttttaccaa ttgaaacatc gccagacaaa acactttcaa
    12841 tacctaaacc acttaacaaa cctgccaata atcgtgttgt cgtaccagaa ttacctgtat
    12901 acaatacttg atgtggcgtg ttaaaagatt gatatcctgg ggaagtcaca actaattttt
    12961 catcatcttc tttgatttct acacctaaca gtcggaaaat gtccatcgta cgacgacaat
    13021 cttcgccaag tagtggctta tatatagtag atacaccttc agctagcgac gccaacatga
    13081 ttgcacggtg tgtcattgac ttatcgcccg gcacttctat ttcgcccttt aacggacctg
    13141 aaatatcaat gatttgttca tttaccattt cattcaccta cttaaaatat gtttttaatt
    13201 gttcacatgc atgttgtaat gttagttgat caacatgttg tacaacgata tctccaaatt
    13261 gtctaatcaa gaccatttgt acaccttgct tatcattctt tttatcactt agcatatatt
    13321 ggtataacgt ttcaaaatcc aagtcagtta tcatgtctaa aggatagccg agttgtatta
    13381 aatattgaat ataatgatta atatcatgct tagaatcaaa caaagcattc gcaactataa
    13441 attgatagat aatgccaacc atcactgcat gaccatgagg tattttatga tagtattcaa
    13501 cagcatgacc aaatgtatga cctaaattta aaaatttacg tacaccttgt tctttttcat
    13561 ctgcaataac aatatccagc ttcgtttcaa tacctttagc aatatattta tccataccat
    13621 ttaatgactg taatatctct ctatctttaa agtgctgttc gatatcttgc gtcgctgatt
    13681 caccattcaa taacgcatgc ttataaactt ctgcatagcc acttaatatt tgctcaaatg
    13741 gtaacgtctt taaaaagtct aaatcataaa tcacagcagt tggacgataa aatgcaccga
    13801 taaggttttt accttgcttt gagttaatac ccactttacc gccaacacta gaatcatgcg
    13861 ctagtatagt cgttggcact tgtataaagt gaacgcctcg taaaagtgtc gccgcaataa
    13921 acccagcaaa atcaccagtt gcaccaccac caacagcaat aattgctgta ttacgagtta
    13981 catgatggga taaaatatac tctaatgttt cttgatattg ctcaaatgtt ttcgtctttt
    14041 caccagctgg aataataact ttatgtacat tttcatatga taaaatatca tcaaatttat
    14101 cagcaaaata ttgatttaca tgctcgtcaa ttaatataaa actttgatca aactgatcaa
    14161 tatacgtgct aatatggtca attgcaccgt gttcaacata tattggataa ttatttgaag
    14221 ggtatgttgt ttgtaatttc atgattacac ctcaattgtt cttgttgtta aaactcaata
    14281 tttaattgtc tgcgctcaat aatttgttgt ttaagttgct caatatgatt tgattggaat
    14341 tcttccaata atgcttttgc tatttcaaat gctacgacat gttcgcagac gatacttgct
    14401 gcaggaacag cacaactatc agaacgttca attgttgctt taaagtcttc tttagtatta
    14461 atgtctactg aatttaatgg tttatataac gttggaattg gtttcattac accattaacg
    14521 ataattggca ttccatttga cataccgcct tctaaaccac ctaagtgatt agatccacga
    14581 taataaccaa tttcactatt atatagaatt tcatcttgaa tctcactacc tggcttttca
    14641 gctgctttaa atccttcacc aaagcttaca cctttaaaag catttatgct gacaacacct
    14701 tgtgcaatct taccatctaa cttacgatca taatgcacat aactacctac accaacaggc
    14761 atattttcaa ctacaacttg aacgacaccg ccaattgaat ctccttcatt tttagcttcg
    14821 tcaattttat ctcgcattgc ttgtgcgata ctgtcatcaa ttacacgaac atcattacga
    14881 tcaagatttg ctttaaatgt ttctgaatca taaaaatctt tatctttaat tccacctatt
    14941 tcaacaacac gactgtatat atcgatatct aactgttgta ataacacttt acataaggca
    15001 ccgactgcaa ctcgagctgc tgtttctcta gcagatgatc gctctagcac atttcgtaaa
    15061 tcacgatgat tatatttcat acctccaacc aaatctgcat gaccaggtct tggttttgta
    15121 atagtacgtt tcatattttc acgttcttct tcacttattg gagctgctcc cataattttt
    15181 ctccaatgcg taaagtcatc attggttaca accatagtaa ttggactacc taatgtataa
    15241 ccatttctaa cgcctgatac tatttctact gtatctttct caatttgcat acgacgtcca
    15301 cgtccgtaac cgccttgacg cttaaacatt tctttattaa tatcctcaac cttaatttct
    15361 atatttgcag gtacaccttc aacaataact gttaattgag gtccatgtga ttctcctgat
    15421 gttaggtatc tcatacacac tcgctccttt catacttcca cgataacatt ataatatgtt
    15481 gttaatctta tcatattcat tgtgcattca taaatgtttg ttttcaagct caattaagaa
    15541 aatggatgac atctcatttt aaatgtcaga attatcttaa tattaaacga actaaaatta
    15601 attataatat attcgtctgt agaaaagtat catgaatttc taaacagttg catcatataa
    15661 tcattaatta aaaatgatta gccgttttaa tataatcgta tcgcaacttt agcaaataat
    15721 atactgacta catttcttaa tacaaaagaa cgaactgtta attattaata aagttaatca
    15781 gtccgtttaa aaaaattatg atattaaaat atacatatgt taagcgcttt tgttatactt
    15841 tgacttcaaa aatataatca attcttttac taatacataa aaaatataaa tgagagtcag
    15901 tataaaaata atttttgtta taggattaag tggttta
//
LOCUS       DAKTIP010000042        15937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAKTIP010000042 DAKTIP010000000
VERSION     DAKTIP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.042"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1102
                     /gene="scpA"
                     /locus_tag="O0305_001040"
     CDS             <1..1102
                     /gene="scpA"
                     /locus_tag="O0305_001040"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCY0274191.1"
                     /translation="ANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSYVTS
                     LDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEGNIDYIVTISPKITKYSSS
                     SSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKKLKK
                     TESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYINKLENFKIRETQGNNGWCAG
                     YTMSELLNATYNTNKYHAEAVMRFLHPNLQGQRFQFTGLTPREMIYFGQTQGRSPQLL
                     NRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGHAMAVVGNAKLDNGQEVIIIWNP
                     WDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1133..1459
                     /locus_tag="O0305_001041"
     CDS             1133..1459
                     /locus_tag="O0305_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCY0274192.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
     gene            complement(1751..1924)
                     /locus_tag="O0305_001042"
     CDS             complement(1751..1924)
                     /locus_tag="O0305_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCY0274193.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(1905..2507)
                     /locus_tag="O0305_001043"
     CDS             complement(1905..2507)
                     /locus_tag="O0305_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCY0274194.1"
                     /translation="MSFVTENPWLMVLTIFIINICYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(2778..3599)
                     /gene="nadE"
                     /locus_tag="O0305_001044"
     CDS             complement(2778..3599)
                     /gene="nadE"
                     /locus_tag="O0305_001044"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCY0274195.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(3592..5061)
                     /locus_tag="O0305_001045"
     CDS             complement(3592..5061)
                     /locus_tag="O0305_001045"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCY0274196.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            5245..6321
                     /locus_tag="O0305_001046"
     CDS             5245..6321
                     /locus_tag="O0305_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCY0274197.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            6341..7135
                     /locus_tag="O0305_001047"
     CDS             6341..7135
                     /locus_tag="O0305_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCY0274198.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNFFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            7207..7314
                     /locus_tag="O0305_001048"
     CDS             7207..7314
                     /locus_tag="O0305_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274199.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(7325..8887)
                     /locus_tag="O0305_001049"
     CDS             complement(7325..8887)
                     /locus_tag="O0305_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCY0274200.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGYEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(9092..10189)
                     /locus_tag="O0305_001050"
     CDS             complement(9092..10189)
                     /locus_tag="O0305_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCY0274201.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGRRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVIIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNTFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            10561..11121
                     /locus_tag="O0305_001051"
     CDS             10561..11121
                     /locus_tag="O0305_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCY0274202.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            11174..12103
                     /locus_tag="O0305_001052"
     CDS             11174..12103
                     /locus_tag="O0305_001052"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCY0274203.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(12298..12471)
                     /locus_tag="O0305_001053"
     CDS             complement(12298..12471)
                     /locus_tag="O0305_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274204.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            12523..13902
                     /locus_tag="O0305_001054"
     CDS             12523..13902
                     /locus_tag="O0305_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCY0274205.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSAQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(14022..15050)
                     /locus_tag="O0305_001055"
     CDS             complement(14022..15050)
                     /locus_tag="O0305_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCY0274206.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            15320..15721
                     /locus_tag="O0305_001056"
     CDS             15320..15721
                     /locus_tag="O0305_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCY0274207.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(15739..15937)
                     /locus_tag="O0305_001057"
                     /pseudo
     CDS             complement(15739..15937)
                     /locus_tag="O0305_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5381 a         2789 c         2350 g         5417 t
ORIGIN      
        1 tgcaaacgct gagagcaatt caaatatcaa agcaaaagat aagaagcatg tgcaagttaa
       61 tgtcgaggac aagagtgttc ctactgacgt acgtaattta gcgcaaaaag actatctttc
      121 ttatgttact tcacttgata aaatctataa taaagagaaa gcctcgtata cacttggtga
      181 accttttaaa atttataaat tcaacaaaaa gagtgatggt aattattatt ttccagtttt
      241 gaacactgaa ggtaatatcg attatatagt tacaatttca ccaaaaatca ctaagtattc
      301 tagttcatct tctaaataca caatcaatgt ttctccattt ttatctaaag tcttaaatca
      361 atataaagat caacaaatta ctattttgac aaactcaaaa ggttactatg tggttactca
      421 aaatcataaa gctaaactag tactaaaaac accacgtcta gaagataaaa agttaaaaaa
      481 gactgagagt atacctactg gcaataatgt aacgcaacta aagcaaaaag cttctgtcac
      541 tatgccaact agtcaattta aaagtaataa ttatacttac aacgagcaat atataaataa
      601 acttgagaat ttcaagataa gagaaactca aggtaacaat ggttggtgtg caggctatac
      661 gatgtctgaa ttactaaatg cgacttataa tactaacaaa tatcatgcag aagctgtgat
      721 gagattttta cacccaaact tacaaggaca acgattccaa tttactggtt taacgccaag
      781 agagatgatt tattttggac aaactcaggg aagaagtcca caacttctta atagaatgac
      841 tacatataat gaagttgaca atcttactaa aaataataaa ggaattgctg ttttaggtag
      901 tagagtagaa tcaagaaatg gtatgcacgc agggcatgcc atggcagttg ttgggaacgc
      961 taaattagat aatggacaag aggttataat tatatggaat ccatgggata atggttttat
     1021 gactcaagat gctaaaaaca atgttattcc agtatccaat ggcgaccatt atcgatggta
     1081 ttcatctatt tatggttatt aaaatttgta ttttagaaag gtaaataata ttatggaaca
     1141 aattgaatta tttagtattg ataaattcaa atgtaattca gaggctaagt attatcttaa
     1201 tattattgag ggagaatggc atcctcaaga tttaaatgat agtcctttaa aatttattct
     1261 cagtacctca gacgattctg attacatttg caaatatata aatacagaac acaaacaact
     1321 cacattatat aataaaaata atagctcaat tgttattgaa atatttatac caaatgataa
     1381 taaaatacta ctaacaatta tgaacacaga agctttggga acttctccta gaatgacttt
     1441 cattaagcat aagtcataag actatctaga tatgattaag taaactatta atcttattaa
     1501 aggtctctag gaagaaaaaa tttcatgcga tgagattgtt ttagaaaaag cgacgatata
     1561 tataatctaa caataatttt taaaatatta agaaccccca gcatatgctt tatatagctt
     1621 gctgggggtt tctcatacaa tatggatcga gcatcttgca tgtataaaca aataggctat
     1681 tcaaagtaaa attactcaaa ctttgaatag cctttcctct tcaaatagtc tttatttaaa
     1741 ttggattatg ttattggatt ttcttaccta caaaaacgat ttcttgatgt gatacttcaa
     1801 ccgtgccatc tttttgctct tgaaacacgg gtttttcaat acgttttacc ggcatatacc
     1861 cttgcatttt cattcgtgac aaacaatcag caattgtttc attctcattt actttgaatt
     1921 tcatgttcta ctacactttc cagttcttct ggttcataat ctttaagctt tctacgacga
     1981 atgcctttag tccagaatcc accatgtatg tttcgaggtt catacgcaat gataaatgct
     2041 ttcggatcta aattttttat cgtatccatc aatttacgtt catattttct tggtgttaaa
     2101 atttgcatca ccatacgact accatctcta ccaaacgcag catagtgcgt aacgccatat
     2161 cctaaatttc gaagttcatt cggtaaatct aactcatatt ctgctgaagt tacatttaca
     2221 actgtataac ctaatgccag tttttcttct attttcatac caacaatgat acctattgaa
     2281 aaaccaaatg cgtaggcaat aatattttga atatggtcta aattagacat aaccaaacct
     2341 aaaccaacga tataaactaa tacttctaaa aaactaactg atgcagcaat ataacgataa
     2401 cctttcaacg ttaaaattgt tcgcatcgtt aaaaacgtta cataacaaat gttaatgata
     2461 aatatagtta gtaccattaa ccatggattt tctgttacga atgacataag gcgccccact
     2521 ctctttcaat tacactttaa ataccaaact gcttcaacct aaccttaatg aatgcatatt
     2581 aatataatat agtacgaaaa ctctcataaa taaatagcga atttgtcata aataaaaatt
     2641 tattctcaaa tgaacatact tactttagtt catacacttt caatttatac actgaagtag
     2701 tctagctgaa tgtggtttaa tgttattaat tctaactcat atttaattta agtcacacgt
     2761 tagaagaaaa aattaaatta ggattttggc cacgtgtatc ttgtatatgc aagttcacgt
     2821 ttgtgtgcat ttcgtatata atgattttca attacttttt gttcttctgg cgtaactggc
     2881 ttaccttcta aataattatc aatcgcctca taagttacac ctaatgcatc ttcatctgga
     2941 agctgtggtt tatcatcttc taaatcagca gttggcgttt tttcatataa ttcctttggc
     3001 gcaccaagat acgctaataa ttgacgacct tgtcgtttat tcaaaccaaa tataggtgcg
     3061 atatctgcag caccatcacc gtacttcgta taaaacccag ttatattttc agctgaatga
     3121 tctgttccta ctacaatacc ttgtcggttt gaagcaattg aaaattgtac tttcatacgt
     3181 tcacgcgctt tttcatttcc tttttggaaa tctgtaagaa caataccggc ttcttttaat
     3241 gattgcacac tttgatcaac tgcaggctta atattgactg ttactatttc atctggttca
     3301 atgaatcgca aagcttgctc aacttcatca gcatcttttt gaactccata aggtaattta
     3361 actgcaataa acgtacaatc aatgccttct tcacgtaatt cgttaacaga catttgtact
     3421 agttttccaa ctaatgtaga atcctgtcct cctgaaatac ctaacactaa agattttata
     3481 aatgaatgtg attgtacata attttttata aattgcttta attccataat ttcttcagca
     3541 ctatcgatac gctttttcac tttcatttct tgtacaataa cgtcttgtaa tttactcatt
     3601 atcttcttcc atctccttaa cgtgttccgc aacttcaaaa atacgtttat gtttattatc
     3661 ccaacatgcc ttgcttaaat cgactggata ttcttgtgga ttcaggaaac gcttattttc
     3721 atcccaaata gattgtaatc ctagtgctaa atattcacgt gattcatctt ctgttggcat
     3781 ttgatatact aatttaccat tttcataaat attatgatgc aaatcaatgg cttcgaaaga
     3841 ttttataaat ttcattttat aagtatgcac tggatggaat aattttaaag gttgttcatc
     3901 gtatggattt tcattttcca aagtaatata atcgccttct gccttacctg ttttcttgtt
     3961 tataatgcga tatacatttt tcttacctgg cgtcgtaacc ttttcagcgt tatttgataa
     4021 tttaatacga tcactatatg aaccatcttc attttcaata gctacaagtt tatatactgc
     4081 acctaatgct ggttgatcgt atcctgtaat cagctttgta ccaacgcccc aagaatctac
     4141 ttttgcacct tgtgctttca aactcgtaat cgtttcttca tccaaatcat tagacgcgat
     4201 aattttagtt tcagtaaatc ctgcttcatc aagcatacgt cttgcctctt tagataaata
     4261 agcgatatct ccagaatcta atcgaatacc tacaaagtta attttgtcac ctaattcttt
     4321 tgcaactttt attgcatttg gcacgccaga ttttaaagta tggaatgtat ctactaggaa
     4381 cacacaattt ttatgtcttt cagcatattt tttgaaggca acatattcgt ctccataagt
     4441 ttggacaaat gcatgtgcat gtgtaccaga cacaggtata ccaaataatt tccccgccct
     4501 aacattactt gtagaatcaa agcccccgat gtaagcagct ctagcgcccc acaatgctgc
     4561 atcaatttct tgcgcacgac gtgtaccaaa ctccattaat ttatcatttg atgcaatttg
     4621 acgaattctg ctagcctttg ttgtaattaa tgtatggaaa tttacaatgt ttaataaaat
     4681 tgtttctatt aattgcgctt gaatcaatgg tgcttctacg cgtaacaatg gttcgttacc
     4741 aaagcataat tcgccttctt gcatcgaacg gatgctgcct gtgaatttta aatcttttaa
     4801 atatgataag aaatcatcct tgtagccaat agactttaaa tattccaaat cagattctga
     4861 aaatccaaaa tgttctataa aatcaatgac gcgttttaaa ccattaaaaa cagcatagcc
     4921 actattaaat ggcatttttc taaaatacaa atcaaataca gccatttttt catgaatatt
     4981 atcattccaa taactttcag ccatatttat ttgatataag tcattatgta acattaaact
     5041 gtcgtcttct aattggtaca cttgtatctc tccaatcgac ctaaatattt tcttacattt
     5101 tatcataatt cattttttta tatacataag agccccttaa tttccatact tttaattaaa
     5161 atcaaccaac aatttaatga catatacata atttttaaga gtattttaat aatgtagact
     5221 ataatataaa gcgaggtgtt gttaatgtta tttaaagagg ctcaagcttt catagaaaac
     5281 atgtataaag agtgtcatta tgaaacgcaa attatcaata aacgtttaca tgacattgaa
     5341 ctagaaataa aagaaactgg gacatataca catacagaag aagaacttat ttatggtgct
     5401 aaaatggctt ggcgtaattc aaatcgttgc attggtcgtt tattttggga ttcgttaaat
     5461 gtcattgatg caagagatgt tactgacgaa gcatcgttct tatcatcaat tacttatcat
     5521 attacacagg ctacaaatga aggtaaatta aagccgtata ttactatata tgctccaaag
     5581 gatggaccta aaattttcaa caatcaatta attcgctatg ctggctatga caattgtggt
     5641 gatcctgctg aaaaagaagt tacacgctta gcaaatcact taggttggaa aggaaaaggt
     5701 actaattttg acgtgttacc actgatttac caattaccta atgagtcagt taaattttac
     5761 gaatatccta cttcattaat taaagaagta cctattgaac ataatcatta tccaaaatta
     5821 agaaaattga acttaaaatg gtatgcagtc cctatcattt ccaatatgga cttaaaaatc
     5881 ggtggcattg tatatccaac tgcacccttt aacggttggt atatggtaac tgaaattggc
     5941 gtacgtaact ttattgatga ttaccgttac aatttactag aaaaagttgc agatgcgttt
     6001 gaatttgata cacttaaaaa taattcattt aataaagatc gagcacttgt tgaattgaac
     6061 tatgctgtgt atcattcctt taaaaaagaa ggcgtatcaa ttgtcgatca tttgaccgct
     6121 gcaaagcaat tcgaactatt cgaacgtaac gaagcacaac aaggtcgtca agttaccgga
     6181 aaatggtctt ggctagcacc gccattatct ccaacattga cgtcaaatta tcatcacgga
     6241 tatgacaata cagtaaaaga tccaaacttt ttctataaaa agaaagaatc aaatgctaac
     6301 cagtgccctt tccatcatta agttagtaga ggtgtagcat atgcaattat attatttagg
     6361 ccctaaaggt acattttcat atttggcttg tcgtcagtat ttttctgaaa atgaagcaac
     6421 ttttcaacca aagtcaaatt tttttgaagt catcaaggca gttgcagatg atgacacatc
     6481 gattggtgtt gtccctattg aaaattctat agaaggaaca atcaacattg tggcagatgc
     6541 acttgcacag caagatgtct ttgcacacgg tgaaatacgt ttagatatta actttgcatt
     6601 atatggtaat ggaacagatt ccatttctga tattaaaaaa gtgtattcta ttgcaccagc
     6661 aatcagtcaa acaacaaatt acatacatca acatcaattt gactatgatt atgtcgacag
     6721 tacaattcaa agtttgacta aaattgaaaa tggcgttgca gcaattgcac ctttaggtag
     6781 tggcgaagct tatggtttta cacctattga tacacatatt gaagactatc cacataacgt
     6841 gacacgtttt ttagttataa aaaatcagca gcaatttgat caaaatgcga catcattgat
     6901 gtttttaatt acaccaatgc atgataaacc tgggttgtta gcaagtgtgc ttaatacatt
     6961 tgctttgttt aatattaatt tatcctggat tgagtctcgt cctttaaaaa cacaattagg
     7021 tatgtatcga tttttcgttc aggctgattc tgcaattaca accgatataa agaaagtcat
     7081 tgctatttta gaaaccttag atttcaaagt agaaatgatt ggtgctttta attaaacttt
     7141 attttctagg ttctacaatt taaataaaat aaagagtcaa ccaaggatta aaataggaat
     7201 ccatccttgg ttgactttct tttgtttgta ttgtttttac acgatgctgc ggtatacgtt
     7261 tcgttaccac tcgtccaagc aattgctaat gtaacctttt atttttcgtt ctaatctgca
     7321 attactattt caatggcagt ggttgattta tatcaatacc taaaactata ggcattacat
     7381 aatacacgac taaaataata attattgcac tgattaagtt tacccagaat cctactgatg
     7441 ccatttgttt gatagatatt ttaccagaac caaagataat tgcattcggt ggtgtcccta
     7501 ctggtaacat gtatgcacag ttagccgcca tagctgcagg tgccataagt agtaatggat
     7561 gcactccaac agcaacagac aacgttgcta aaatcggtaa aatcatcgtt gcagttgcag
     7621 tattagatgt cacttcagtt aaaaataaga caaagattgt tatgacaatt acaataagaa
     7681 tcggactaac accatttaat gatttcaact gttcgcctaa ccattttgct aaaccacttt
     7741 cagaaatacc tttcgctaat gctaaaccgc caccaaataa aattaataca ccccaaggga
     7801 gctcttttgc aacttcccag tcaatgatac ggcgatgttt ttcagtattt ttagctggaa
     7861 taataaataa taatattgat ataaaaatag caatcgtacc atctgcaaca gatgacgtaa
     7921 cttcccattt tttcagaaga aactctcttg taatccataa taagctagca agtacaaaga
     7981 tagtttgtac taccttttct tcatacttca ttttgccaag ctcgtctaac ttttgtttaa
     8041 ttaacgtctg accaccaggt aaatatttca aatcatgtct aaacgcaaca tatcttaaat
     8101 agagccaagt aatacctaac aaaacaatga ccgttggaat ccctacaatc atccatttag
     8161 caaaactaat ttcatatcca aaatgttgca tgtattgtcc ttttaaaata attaatggcg
     8221 gggttccgat taatgtaccc aagccaccaa tcgtacctgc atagccaatt gctaaaacta
     8281 gagatttttc aaacttttga atacttgttt gattcgtatt ggcttcttgt aaatcatgtg
     8341 cttccttaat aattgctaaa ccaatcggaa tcataatcat tacagctgca gtgttcgata
     8401 caaacataga taagaatcct gttgccacca tgaatccaag taatattttt gaagtgcttg
     8461 caccaatcaa attaataatt gtcaacgcta ctctcgtatg taaattccat ctttccattg
     8521 caattgccaa aataaatcca cctaaaaaca aaaagataat atcattgcca tattcggatg
     8581 atacttgttc tggtgtaagt atatgaccta atggtaataa cacaattggt aataagctcg
     8641 ttgctgcaat aggaattgct tcagtaatcc accaagtcgc aatccataaa gtaatcgcta
     8701 aaacatagac gcctttccaa ggtaagtctt gtggatgaaa gaataataat gttaatagga
     8761 aaagtaatgg acctaatatt agtccaataa gttgtcccgc actaaacggt ttctttttct
     8821 ttgattttga aaagaatgtt aaataccttt tcgatatcat tgattgatgt tgattgaaat
     8881 aagccataaa tttccccctc atgatgatta tgacaagcta cttcaactca ctatattaga
     8941 gtatagaaag ttgtctatct tgtattatca ttaccgtcaa aatacccctt ttatcagata
     9001 attgggttta ttatagcata tttataccgt tttatgaatg cgctttcaaa aattatctac
     9061 tattcttaat cctaatgtca tgcgaaactt tttacttttt tatttcatct acattgattg
     9121 tagtaacttg aatacccgct tcaacaggac ttaaaaacac tgtatcaata tcttctaaat
     9181 gaattgattg agtcgaatta ttgaatgtat tcccaacgat taatacatct ttatgtttaa
     9241 cattattata attacgtaca tgtatcgcat ctttagacat tgatccttta aattcatttc
     9301 caattatatt catatttatg cctgcttggg aacctaagtc ttttcctgtc atcacatctg
     9361 ctgcattttt accatctcta actcctaaat atctaatgcc accctcacag ttaataaact
     9421 tattattaat aatgaaagca tctttatact tcaagggagt taatgcataa ccttgtatat
     9481 cttcaaatat attatttcta atatgaatat tctcatagta tcgattatgt ctacttgcat
     9541 gtgagccaat agcacgattc caacttccca tttcgggcaa ttctgaaggt ccaaaataac
     9601 aatcttcgat aatgacattt ttcgttatcg taccatctgt cgttccgaat tttggaaaag
     9661 caccaggtac ttgaatgtct aattgtattg cttcagaata aaagcgttcg ccactatagt
     9721 ctatgaatcc ttcaaatgaa cagcttttaa tatagagtcc gttaatccca caagcatcaa
     9781 ttgcatgacc actcactaca ttcttaatgg tcacacctat taattgaata tcttcagcat
     9841 gcccaatgca catagctgta ttgttataag gatacgatac accattcata tcaaacttgc
     9901 cgcctttaat atgaatatga ctgtgtccat tataaccacg ataaaaacca aagcgacgac
     9961 cattttttaa taaaggacca gaatgacatc ggcgtaaaat agtttcatta tctaacaaaa
    10021 gtgttgtatt gccatatatc gttaatggtt tgcaaatatc atacgtccct ttcggtatat
    10081 aaactgtcgt tggtttacat cttccacgat ttaacgcacg ttgtattgct cgagtatctt
    10141 tagttttgtt tatgcctgtt aacccataat ctttcatatt gatatacata aatcgtcatc
    10201 ctttgtctat tttctaatta ctcatatatt aaccaattat tcaaattata atcgtgttag
    10261 gttgatttaa cagatagtca taaatctcgt gatgccatct attagcattt ttatattcaa
    10321 aacatcaaca cttgatatta gttctaagtt tgtcacattt agtattctat tacagctatt
    10381 ttaaaactat cgcaaaaact tcttatatta tttgttgatt gcgtgatttg ctcttccaaa
    10441 aaagttgatt aatctatata cttaatctat atttatatat taaccattag ggttaaaaat
    10501 tactctagca tttatgaata gatgggagtt tattttatta ttatatagga gagatgttga
    10561 atgacacatc gcgcactatt agttgttgac tattcatatg actttatcgc agacgacggc
    10621 ttactaacat gcggtaaacc tggacaaaat attgaagatt ttattgtttc tcgtatcaat
    10681 gactttaatt attatcaaga ccatatattc tttttaatgg atttacatta tttacatgac
    10741 attcatcatc ctgaaagtaa attattccca ccacacaata tcgtagatac aagtggtaga
    10801 gaattatacg gtaaagtagg taaattatac gaaacaatta aagcgcaacc taatgtacat
    10861 ttcattgata aaacgcgcta tgattcgttc tttggtaccc cgcttgatag tttattgaga
    10921 gaaagaagta ttaatcaagt cgaaatcgtt ggtgtatgta ccgatatttg cgtgttacat
    10981 acagcaattt ctgcatacaa cttaggttat aaaatttcag tacctgctga gggagtggct
    11041 tcatttaatc aaaaagggca tgaatgggca cttgcacatt tcaaaaactc attaggtgca
    11101 gaggtagaac aacacgttta aatcgtgcta aaataattat aaagaataca atttacaagg
    11161 gagatatttg acaatggcta aaacatatat tttcggacat aagaatccag acactgatgc
    11221 aatttcatct gcgattatta tggcagaatt tgaacaactt cgaggtaatt caggagccaa
    11281 agcataccgt ttaggtgatg tgagtgcaga aactcaattc gcgttagata catttaatgt
    11341 acctgctccg gaattattaa cagatgattt agatggtcaa gatgttatct tagttgatca
    11401 taacgaattc caacaaagtt ctgatacgat tgcctctgct acaattaagc atgtaattga
    11461 tcatcacaga attgcaaatt tcgaaactgc tggtccttta tgttatcgtg ctgaaccagt
    11521 tggttgtaca gctacaattt tatacaaaat gtttagagaa cgtggctttg aaattaaacc
    11581 tgaaattgcc ggtttaatgt tatcagcaat tatctcagat agcttacttt tcaaatcacc
    11641 aacatgtaca caacaagatg ttaaagcagc tgaagaatta aaagatattg ctaaagttga
    11701 tattcaaaag tacggcttag atatgttaaa agcaggtgct tcaacaactg ataaatcagt
    11761 tgaattctta ttaaacatgg atgctaaatc atttactatg ggtgactatg tgactcgtat
    11821 tgcacaagtt aatgctgttg accttgacga agtgttaaat cgtaaagaag atttagaaaa
    11881 agaaatgtta gctgtaagtg cacaagaaaa atatgactta tttgtacttg ttgttactga
    11941 catcattaat agtgattcta aaattttagt tgtaggtgct gaaaaagata aagttggcga
    12001 agcattcaat gttcaattag aagatgacat ggccttctta tctggtgtcg tttctcgaaa
    12061 aaaacaaatc gtacctcaaa tcactgaagc attaacaaaa taatactata ttactgtcta
    12121 attatagaca tgttgtattt aactaacagt tcattaaagt agaatttatt tcactttcaa
    12181 tgaactgttt tttatttacg tttgactaat ttacaaccct ttttcaatag tagtttttat
    12241 tcctttagct accctaaccc acagattagt gatttctata caattcccct tttgtcttta
    12301 cattttctta aaatatttgc gatgttgagt ataaattttt gttttcttcc tacctttttc
    12361 gttatgatta aagttataaa tattattatg tacacgattc atcgctctat tttcaacttt
    12421 caacatatat aattcgaaag accatttaaa attaacggcc acaacattca aatcaattaa
    12481 tcgctttttc caaaataatc atataaggag gttcttttca ttatgaatat cattgagcaa
    12541 aaattttatg acagtaaagc ttttttcaat acacaacaaa ctaaagatat tagttttaga
    12601 aaagagcaat taaagaagtt aagcaaagct attaaatcat acgagagcga tattttagaa
    12661 gcactatata cagatttagg aaaaaataaa gtcgaagctt atgctactga aattggcata
    12721 actttgaaaa gtatcaaaat tgcccgtaag gaacttaaaa actggactaa aacaaaaaat
    12781 gtagacacac ctttatattt atttccaaca aaaagctata tcaaaaaaga accttatgga
    12841 acagttttga tcattgcacc atttaactat ccttttcaac tagtgttcga acctttaatc
    12901 ggtgctattg cagcaggtaa tacggcaatt attaaaccat ctgagttgac gcctaatgtt
    12961 gcacgagtga ttaaacggtt aatcaatgaa acatttgatg caaattacat tgaagttatt
    13021 gagggaggaa ttgaagaaac gcaaacgtta attcacttac cttttgacta tgtcttcttt
    13081 acaggaagtg aaaatgtagg caaaatcgtt tatcaagctg ccagcgaaaa tttagttcct
    13141 gtgacattag aaatgggcgg taaatctcca gtcatcgttg atgaaacagc gaatattaaa
    13201 gttgctagtg agcgcatttg ttttgggaaa ttcactaatg ccggccaaac atgtgttgca
    13261 ccagattaca ttttagtaca cgaatctgta aaagatgatt taatcacagc cctatcaaaa
    13321 acgttgcgtg aattttatgg tcaaaatata caacaaagtc cagattatgg ccgcattgta
    13381 aaccttaaac attatcatcg tctgacttca ttacttaaca gtgcacaaat gaatattgta
    13441 tttggtggtc atagtgatga ggatgaacgt tatatagaac caacattgtt agatcacgtt
    13501 acaagtgatt cagcaattat gcaagaagaa atttttggtc ctatcttacc gattttaacg
    13561 tatcagtcat tggatgaagc aatagccttt attcaccaaa gaccaaaacc tttgagttta
    13621 tatttattta gcgaagatga aaatgctaca caacgtgtaa taaacgagct atcatttggc
    13681 ggcggcgcta ttaatgatac attgatgcac ctagcgaatc ctaaattacc atttggtggt
    13741 gttggcgcct caggtatggg acgctatcat ggtaaatatt cattcgacac ttttacacat
    13801 gaaaaaagct acattttcaa atctacacga ttagaatcag gtgtccattt accaccatat
    13861 aaaggtaaat ttaaatacat caaagctttc tttaaaaatt aatttttgag acaaaaataa
    13921 accatgcttc cataatttga tgttcttgaa aacaataaat ttcaaggtat acacaaattt
    13981 tttggaaaca tggttttctt tttatcatga ttaattaaag attaaaatat gacacataca
    14041 ccttcagatg ctacacgagt gttatcacat agcgtaattt tacctgtttc tttatttctt
    14101 tcgaaaacag ttacaactga atctccttgc tcatgagcac aaactaaaag gtcatctgat
    14161 gaggcaatat taaaatctct tgggaattga ccaccacttt cagtaattgt tactagttct
    14221 aagtgttgac cattatcaag aactttaaaa attgcaatgc tatcatgccc tctattagat
    14281 acatataaga attgttgatc atgagataaa cgcactgctg caagtttagt atctccatca
    14341 aagttttcag gaattgttaa atgacgctcg agctcttcaa atttaccgtc attatattct
    14401 gcaacactca cagtatttga taattcgtgt acgacataag caaattttcc attatcatga
    14461 aattcaatat gtcttgtccc atcactatct ttaaataaag attctttata aaattcaaac
    14521 ccgttgtcat caaatttata agtaacgata cgatcagcac ctaaatctgt tactgcaacg
    14581 tacttaccat ctggagtttg attaatataa tgtgcgtgtg gatgatcttg tctttcatgt
    14641 gtacctgttg gaaaatcatg tgcaagttct tgaattagac gtataatttc acctgtgtgc
    14701 gtatttaatt catacatacg tatgatgcca gcaccatata ccgcttcaaa taaatatcgt
    14761 ttatcttctg aaatcgatac ataacaacct gtaccagctt ttgaagacaa acatttattt
    14821 aataaatgca attcaccatt gtcatcaatt ttcaaacttg caacaccgca ttgttctcct
    14881 tctttgttga ttccatataa aacttcatta ttacgcacca aatatgtaga cgcttctaat
    14941 tcaaatcctg tttctaataa atcaatacgt gactgatttt cgtttaattc aaaacgataa
    15001 atccctttac cattcttttt agtgtaagaa ccaatatatc catttgtcat tacttttacc
    15061 cctccatgtt agtcttattt tcattttatt aaattttagc tagaattaac aatcatttat
    15121 catttcctta aagctaaaac tatttaacta ctttaaaaga actatttgct gtgatatgtc
    15181 attattcgat tttaattaat ccctcttttt tacttacacg agcattaatt attaacgcgt
    15241 ttatgcttcc acaaaatttt tgttgcaaaa tagaacacat gttcgtataa tgtaattatc
    15301 agattggggt gaattaagga tgacaagtta tgaagaagaa actgattatc gtaacgttcc
    15361 aagagaatta ctagataaca atatacctat gggaagaggc atgattaaat gggctccttt
    15421 tgcaacatta cccgaacaat tcgaaacgat tcaacaatac attatcgacc aaaacaaaat
    15481 tacacgtcct gtattatcag atgatcaatt ggctgagcta aacattcggc tacacgaggc
    15541 gctacaatat gcccaacctg tagaagttaa attttataat aatggctttg tagattcagt
    15601 acgcttaacc atttatcgta ttgatgctat taattatgaa attgatggtt atgtttacaa
    15661 tcagcaacaa cgtcaaaaaa tttctatctt tgatatctta gatattgtct tgttacctta
    15721 atgctttgaa cataattaaa cagattgagt atatgcatat aagatgattt ttaacatcat
    15781 ttttggatga taggatgttg cgccacgatg atgtctgaat tcatcgaatt cgctatcagg
    15841 tattgtttca acaatttcat taacatatcg tgaaatatca ttttgaggga ttctaacaga
    15901 agtttctatt ggtagtgtag ttggggcatg ttataat
//
LOCUS       DAKTIP010000043        15791 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAKTIP010000043 DAKTIP010000000
VERSION     DAKTIP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15791
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.043"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            82..522
                     /locus_tag="O0305_001058"
     CDS             82..522
                     /locus_tag="O0305_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCY0274208.1"
                     /translation="MNTKYFLAAGAVITTLALGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            864..1337
                     /locus_tag="O0305_001059"
     CDS             864..1337
                     /locus_tag="O0305_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0274209.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            1312..1998
                     /locus_tag="O0305_001060"
     CDS             1312..1998
                     /locus_tag="O0305_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0274210.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            1998..3053
                     /gene="saeS"
                     /locus_tag="O0305_001061"
     CDS             1998..3053
                     /gene="saeS"
                     /locus_tag="O0305_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCY0274211.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCNEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENEDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(3125..4108)
                     /gene="csbB"
                     /locus_tag="O0305_001062"
     CDS             complement(3125..4108)
                     /gene="csbB"
                     /locus_tag="O0305_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCY0274212.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSLENDEKDTHKVYSK"
     gene            complement(4240..5079)
                     /locus_tag="O0305_001063"
     CDS             complement(4240..5079)
                     /locus_tag="O0305_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0274213.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(5301..6650)
                     /locus_tag="O0305_001064"
     CDS             complement(5301..6650)
                     /locus_tag="O0305_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCY0274214.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(6871..8052)
                     /gene="nagA"
                     /locus_tag="O0305_001065"
     CDS             complement(6871..8052)
                     /gene="nagA"
                     /locus_tag="O0305_001065"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCY0274215.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(8360..10318)
                     /locus_tag="O0305_001066"
     CDS             complement(8360..10318)
                     /locus_tag="O0305_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0274216.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAAPEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLTIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFNPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YAFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(10324..11244)
                     /gene="pfkB"
                     /locus_tag="O0305_001067"
     CDS             complement(10324..11244)
                     /gene="pfkB"
                     /locus_tag="O0305_001067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCY0274217.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(11241..12005)
                     /locus_tag="O0305_001068"
     CDS             complement(11241..12005)
                     /locus_tag="O0305_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0274218.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(12255..12737)
                     /gene="ybaK"
                     /locus_tag="O0305_001069"
     CDS             complement(12255..12737)
                     /gene="ybaK"
                     /locus_tag="O0305_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCY0274219.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(12911..13363)
                     /locus_tag="O0305_001070"
     CDS             complement(12911..13363)
                     /locus_tag="O0305_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274220.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(13656..14822)
                     /gene="norA"
                     /locus_tag="O0305_001071"
     CDS             complement(13656..14822)
                     /gene="norA"
                     /locus_tag="O0305_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCY0274221.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANDYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            15031..15453
                     /locus_tag="O0305_001072"
     CDS             15031..15453
                     /locus_tag="O0305_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0274222.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            15639..>15791
                     /locus_tag="O0305_001073"
     CDS             15639..>15791
                     /locus_tag="O0305_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0274223.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPN"
BASE COUNT         5232 a         2921 c         2180 g         5458 t
ORIGIN      
        1 gaagttaaga attagttaat ggcatattat ttgccttcat tttaaactta acttatcaaa
       61 ttgaagaaat gaggagttag catgaataca aaatattttt tagcagctgg tgctgttatc
      121 acaacattag ctttaggtgc ttgtggtaat tctaattcac aagatcaagg taacaaaact
      181 gaacaaaaaa caaagtcaga agatagcaat gttaaaactg ataaaacaaa acacctaaca
      241 ggtacattca gttctaaaaa cggtgaaact gttgaaggta aagctgagat taaaaatggt
      301 aaattaatgc ttactaacta caaatcatca aaaggtccag atttatacgt ctacctaaca
      361 aaaaatggcg acattaaaaa cggtaaagaa atcgcaatgg ttgactacga taaagaaaaa
      421 caaacatttg atcttaaaaa tgtagattta agcaaatatg atgaagtaac aatctattgt
      481 aaaaaagctc acgtcatctt cggcggcgct aaattaaaat aatcaatttc tgagttaaac
      541 ttttatttac aacatactat tactatacat tacaaatttt taaaatatac attacactca
      601 ttactcaatg gaaagcgtat gatttcacag cccccctagc ttgtagaaat catactttcc
      661 ttttttcaat atatatacaa ctattaaatc ccataagatt gcagagcaca taagtaaatt
      721 tttttagagc ttgaggtttg tttagcttaa gcaacccatg agctcaaaca cttcctgttc
      781 acataacact acaaatcgca ttatgttgct taatcttatg tttatataaa ttacacacaa
      841 taaatagaaa gaatgtgaac atcatgaata aattattgct actcgttaca tttatcattc
      901 gtgtgggttc aggtattgtt atgttaatgc aaggctacga aaaattaacg ggcggattta
      961 cgctgaaagg tttagtacca gtcatcgcta acaatactga ttcaccagag tggtataagt
     1021 ggtttttcgc aaatatagtt gcacatacga cgtcattatt tgatattgtt gtcccactcg
     1081 gagagattgc aattggatta ggtttaattt ttggagtttt tgcatatgct gctagtttct
     1141 ttggagcctt tgttatgata aattatatct tagcagatat gatatttacg tatcctcttc
     1201 aattaacttt ctttatcctt ttactaatga gtcactcatt gttaaaacag atttcactta
     1261 aagaaatcat taattacttt agaggtcgta agaacagagg tgaaaaaata gatgacccac
     1321 ttactgatcg tggatgatga acaagacatt gtagacattt gtcaaaccta ttttgaatat
     1381 gaaggttaca aagtaacaac gacaactagc ggtaaagaag caatttcttt actatcaaat
     1441 gatattgata tcatggtact tgatatcatg atgccagaag ttaatggtta cgacattgtc
     1501 aaagaaatga aaaggcaaaa attagatatc ccctttatct atttaactgc caaaacacaa
     1561 gaacatgata ccatttacgc cttaacttta ggtgcagatg actatgtcaa aaaaccattt
     1621 agtccaaggg aactcgtttt acgtattaat aatttactta caagaatgaa gaaataccat
     1681 catcaaccag ttgaacaact gtcgtttgat gaattaacac ttattaactt aagtaaagtt
     1741 gtgactgtaa atggtcacga agtccctatg cgtattaagg aatttgagtt attgtggtat
     1801 ttagcttcta gagaaaatga agttatttct aaatcagaat tacttgaaaa agtttgggga
     1861 tatgactatt acgaagatgc taataccgtg aatgtccata tacaccgtat tagagaaaaa
     1921 ttagaaaaag agagctttac aacatatacc atcacaactg tatggggatt aggatataaa
     1981 tttgaaagga gccgataatg gtgttatcaa ttagaagtca aatcattatt ggcgtcgtat
     2041 cgagtatact attaacttca actattttag caattgcata tattttaatg tggtttaacg
     2101 gccatatgac actaactttg accttaacga caataattac aagctgttta accttattaa
     2161 tatgtagtat ttttattaat ccacttatac aaaaaattaa gcagtttaat ataaaaacta
     2221 agcaatttgc taacggaaat tacgcaagca atgataaaac gtttaattca ccaaaagaaa
     2281 tttatgaatt aaatcaatct tttaataaaa tggcttctga aattacgcaa caaatgaatc
     2341 aaattaaatc cgaacaacaa gaaaaaacag aactgattca aaacttagcc catgatttaa
     2401 aaacaccttt agcaagcatt atttcatatt ctgaaggact acgtgatggt ataatcacta
     2461 aggatcatga gattaaagag tcatacgaca tattaattaa acaagcaaac agattatcaa
     2521 cattatttga tgatatgact catattatca ctttaaatac aggtaaaaca tatcccccag
     2581 aattaataca actagaccaa ttacttgtat caatattgca accatatgag caacgtatca
     2641 aacatgaaaa ccgcacatta gaagtgaatt tctgtaacga aattgatgca ttttatcaat
     2701 atcgaacgcc acttgagcgt attttaacaa acttacttga taatgcgcta aaattttcaa
     2761 atgttggtag tcgcattgat attaatatta gtgaaaacga agatcaagat actatcgaca
     2821 ttgctattag cgatgaaggt attggcatta taccagaact acaagaacgt atattcgaac
     2881 gtacattcag agtagaaaac tctcgtaata caaaaacggg tggttctgga ttaggcttat
     2941 atatagctaa tgaactcgcg caacaaaata acgcaaaaat cagtgtaagc agtgatatag
     3001 atgtaggaac tacgatgact gtaacattac acaaattaga cattacgtca taatccgatt
     3061 tatttataaa ataaaatgca aagactaaaa agaagctccc attaatgagg gcttcttttt
     3121 ttgtttattt agaataaact ttatgggtat ccttctcatc attttcaaga cttgaaagat
     3181 ttgtagcttg aataatatat ttaggacgtg ccttaacttc ataatatatc ctgccaatat
     3241 attcacctac aacaccaatt gaaattaact gtatgccgcc taataataaa atagctgcaa
     3301 tcgttgaaaa atatcccgga atattaacac cagatatcat aatattgatg aataaataga
     3361 taatatataa cacgcttatt gaaaagataa acaagccaag ataaatcatc attctcaaag
     3421 gtttactatt aaaggaaatc aatccatcaa taccataatt aaataacttt ttaaaggacc
     3481 acttagattc ccctttttgt ctctcaacat tttgatacgt aaacacttta gtattatagc
     3541 ctatccattc aaataaccct tttgaaaatc gattatattc ttcaagtgat gcaatggatt
     3601 taacagctct ttggcttaaa agtctaaaat caccaacacc atcatcaaat tgtacttctt
     3661 ctacaaagca attaactaac ttataataca aatggcttaa tgttttgcga ctaaaatttt
     3721 caccacttct atcacgcttt gcaatcactt gatcatagcc ttccataaaa ccttcaacca
     3781 ttttcggaat atattcaggt ggatgttgca aatcacaatc tatcatgatg actgcatcaa
     3841 attcagtact atgctggtaa cctgcaatca tagctgcttc ttttccaaaa tttctactaa
     3901 aagaaataaa gctgacatgc ctatctattg tggcaagatg ttgcatttca tctatagtgg
     3961 tatccgtact accatcatct atgaaaagca tattataatc atagcctttc acactactat
     4021 cttgtgaaag tatttcagtt aattgttgat gtgtttgtgt aatgacttcc ccttcattaa
     4081 aacaaggaat gacgactcta attttcatct atgtccatcc tttttattta aaagattttg
     4141 aatgtacctg agtattgttt tatcaaatag taaaaaccaa ctcatttatt ttaatgtaca
     4201 cattaaaagg cattcataaa cgtaaaattt aacatccagt tattttaaat ccccaatttt
     4261 cacatcatct ggatttttac cttgtcttgc atttctattt aaagcatcaa tttcagctac
     4321 ttctgttaat tctaaattaa aatctaaaat atctatattt tcttgaatgc gtttaggtgt
     4381 ctgagatttt ggaataataa ttctattgtg tgctaaatgc caacgtaata caacttgtgc
     4441 tggtgtttta tgatattttt cagcaatttt aacaatgaca gggtcgtcta gtagtcctct
     4501 atttctcatc aaaggcatcc atgcagtcac tttaatatcg tgacgatcac aaaattcttg
     4561 taattcttgt tggttaaaat atggatgtac ctcaatttga ttcaccattg gtttgatact
     4621 tgattgagcc attaattttt ctagatgatg aacattaaaa ttacatacac ctattgcttt
     4681 taccttacct tgctcgtaaa gttcttccat agctttatat gtttctaaaa atagaccatc
     4741 tgcttcacaa ggccaatgta ttagaaataa atcaagataa tcagtttgta aattttcaat
     4801 cgatttgttg aaatattcga atgttttctc ataaccttga tagtcattcc ataacttcgt
     4861 tgttataaac aaatcttctc tatcgacgcc attatccttt aatgctcgtc ctagtgaagc
     4921 ctcattatca taaaagtatg ctgtatcaaa cgctctatag cctgcgtcaa ttgcagcatt
     4981 tacaacttta gtcatatctt cgtcagagat tttataaaca cctaacccaa ctgaaggcat
     5041 cgggtatcca ttatttaata tttgtatctc attcaacatc tttatatctc tccaatctat
     5101 gtatctttta tatctttaca ttaccctaaa tttttcaaca aactcaatta atacgaatta
     5161 tcgctttcaa taaaaattat tcattaaatc attaaagata ttgagttcca atactatttt
     5221 cacttttcat taattttagt caaaaaaata accaaccaaa aatgaattaa atcattctta
     5281 gttggtttat atattaatat ctattctgat ttttcatctt catcagactg tccgatagta
     5341 ggtctcgctt cattaaattc atagtttaat atcacccaaa taatttggtg gttatcgatt
     5401 tctgaaacaa cccagcgatc ataagttgta tccacgtaat catctttttg taaattggta
     5461 ttacgagatt gtaaccatcc acctatcgta tcaatatcct cagagtcatc aaattctata
     5521 ccgaactctt cagttaaatc atccaatagt actctgccat ttacttggaa tgtcttatta
     5581 tcaattttaa cgatatcatt cacttcatca tcatcaaatt catcacgaat ttctccaacg
     5641 atttcttcta aaatatcttc catcgttaaa atacctgccg ttccaccata ttcatctata
     5701 ataagactca tatgtacatg ttcacgttgc attctaatta atgcatcact gatacgtgtt
     5761 gtctctgaaa tcattggcaa ctcatgtata tagtttgcta ttttaatcgt ttttccagaa
     5821 gcgtattcag ttaaaaattc tttgacgtta ataaatcctt taatgtggtc tttatcacca
     5881 tcatcagtaa ttggataacg cgtaaattga tgttctttta ttgtttctag taattcgtct
     5941 acattaaaag gttcatttag tgtaatcatt tgagttctag gtaccattat atcttttgca
     6001 tgtctttcat cgaatgaaaa gatattttgc atatatgcca attcagtttg gttgatttct
     6061 ccaccattat aactattgtt aataataatt ttgatttctt cttctgacat tgcatcagtt
     6121 tgggcatcag gatttacacc aaacattcta ataataacac gtgcagaacc attcatcagc
     6181 caaatcaatg gtttcataat gttaccgaaa tagaacaatg gtcttgcata tactaaagca
     6241 agcttttcag tatgttgaat agctatagat ttaggcgcta attcaccaag tactacatgc
     6301 aaatacgtaa cgattataaa tgacactgca aacgaaatcg tcgtcgttaa tgcagttggt
     6361 aaattgattg cttcaaatat tgggtgtaat agcttttcaa acgttggttc accaagccaa
     6421 cctaacccta aagatgttac tgttatacct aactgacaag cagaaagata ataatctaga
     6481 ttagcaatca tcttttttac tattttagca ggtttatttc cttcatctgc tagctgttca
     6541 attcttgttg ctctaatttt tactaatgca aattctgaac caacaaatac agtggttaat
     6601 gcaattaata gaataaatat aatcaaacta attatggtcg aagtttccaa ttaattccct
     6661 atttctaggg attcacctcc atgttatatg tgtcactcat gggtaacacg cattcaaatt
     6721 tatcactatg acttaaatta caactacatg ttatcgcctt cccatttaga cacccccaga
     6781 aaaaaatgtt attgcttcta ttttatcata atataaagtg cttatgttaa cagattaaat
     6841 ctattgcata catttaatta tgattattta ttagctaaat gtgtgaacct tgccttgttt
     6901 tattgtagat tttacattca tatcatcatc tagaataaca agatctgcat ccttatttac
     6961 tttaatacta ccttttctat catcgatacc taatgcaatg gcttgattta aacttgttac
     7021 tcgccataaa tgatctaatg tatcacctgt aaatgatatt aagttacgta acccatgatt
     7081 catttttaaa atactaccag caagcgcacc atttgcaaga cgtgcttgtt gcgattgaac
     7141 agttactttt tgtccaccca aatcatattc tccttcaggc ataccttttg cacgcattgc
     7201 atcggtaatt aaataaaaac gttcattacc tttcatacgg taagcaattg caaccgatgc
     7261 cggatgagaa tgagtgccat caacaatcat ttcggtatgt agagcatcat tcaaccatgc
     7321 tgctccaaaa acacctggtt ctctatgttg gaatggcgtc gctgcattat ataaatgcgt
     7381 gacatgttta gctcctcgct caacagcttc aactgcttct tcgtatgttg ctactgtatg
     7441 accaattgaa aaaataatgt catctttata cgtttcaagc gcttcttttg caccttcaac
     7501 ttcaggtgca aacgtcataa tctttattaa tccgttagca gtctcttgaa aatgtttaat
     7561 tttatcgata aatgggcgta caacgtattg cggatgttga gcaccaactt tattttcaga
     7621 tataaatggt ccttctaaat gtatacctac aatttccgct gcattgtgaa catcttgctc
     7681 cgcttcatat ttagcaatat ttgtaagtgc attatctatt ttatcagtcg attgcgtcat
     7741 tgtagtggcc aagtatgatg tcgtcccttc agacaacaaa ttttcggata gatattttaa
     7801 gccatcgtat gacccatcca ttgcatcttg accataacca ccatgaatat gtatatcaat
     7861 aaaacctggt aatacatgat gacctttagc atcaatcact tgaattttat ttgtcgtatc
     7921 attatcaatt gctgctttat catccacttc tccaattgca acaatctgtc catctttcac
     7981 atgaatgtaa ccattatcga ttttgccatc ttcagtataa actttgccgt tatatataat
     8041 taattctgac acgtataaac ccttctttat ttataaattt tcactttaag acactcatat
     8101 tttactatat ctgcttcgtt tggcataact ttttgcaaaa tattaaaaaa gtctatacat
     8161 ttgtggtgtc tatttcttca ttcatttatc taaaaataaa aacactttca tatcaattca
     8221 taaaaactga attaatagaa agtgttttta cagctttgta taagctaccg ttaacacgat
     8281 atataaaact aacatatatt cattgaacat acaacttaat atgaagctct ttgctaacaa
     8341 tcattttaca tcattaaaac tactcgtcca ttgattttga agcttcgatt tctgtttcag
     8401 ttaactttgg tttgattaaa ccgtaaatta atgctgaaac taatgtgcca actagaagtg
     8461 caataagagt ttgaagtaag tgtgcaccat cagtaccaac aattacaata ataccaccat
     8521 gtggcgcagt aattcgtgaa cctaagccta aagcaattgc gccacctata cctgaaccaa
     8581 tcatcattga aggaataaca cgtaatggat cggcagctgc aaatggaatc gcaccttctg
     8641 taataaatga catacccatc acatagttag ggataattga accacgttgt tcttttgtaa
     8701 atttacgtct aaaaattaac atcgctgtcg caattgctaa cggtggaatc ataccaccaa
     8761 tcattgcagc tgtaattggt gctgcattac cttcaatcaa cgcacctgtt gcaaaaacat
     8821 atgccgcttt gttgaatgga ccgcccatat caatcgccat catagcgcca ataactaaac
     8881 ctaataatac aatattagaa cctgataaat tgtttaatcc atctaataac aaatgattta
     8941 accaagatgc tggtggatta aaggcataaa tcatcaataa gcctgtagcc gtcactgtta
     9001 atagtggata aattaatgtt ggctttaatc cctctaatgc ttgtggtaac ttacgtgtca
     9061 tggctttaac accttgtgtt aagtaacctg ctaagaaacc tgcaataata ccaccaataa
     9121 atcctgaacc acctgaaatt gctaacatac cacctacaag acctgaagcg aaaccaggtt
     9181 tatccgcaat actacgtgca atgaatccag ataaaattgg aatgattaac gcgaatgcac
     9241 ttttactacc aatgttccaa agctgctctg caaacgcatt gtactctgag ctttttggat
     9301 taaatgaatt tgctccaaat aaaaatacaa ttgccattaa aataccacca gatattacaa
     9361 gtggcaacat gttagaaaca ccgttcatta agtgtttata gaatgcttta cctgggctta
     9421 atttttcttg actgtcattt gagttacctt ttctttgacc atcacgggca acaaaaggtt
     9481 tacgacttgt atctaatgct ttattaatta attcttctgg gcgtttaata ccatctgcta
     9541 ctggtacttc tacgacattt ttaccatcga agcgatccgt ctcaacatga acatcagcag
     9601 caacaatgat acctgttgca ttttcaatat cttgttcagt taaatggttt ttaatgccgc
     9661 ttgaaccatt cgtttctact ttaattttaa tacccatttt atccgcttgc tttttcaatg
     9721 catcacgtgc catatatgtg tgtgcaatac ctgttggaca agcagttact gctaacacat
     9781 atggctcatt agattgttta gatgattgtt ctgcagttgc aacttgttgt gcttcagctt
     9841 ctgcctcttc ttcttttgtc acttcatcat cagcctcatc tatgatcgtt agtacttctt
     9901 caggtgatga agcatgtaat aatttctcac gtacattttc atccattaaa ataccagaca
     9961 acttagctaa agcatctaga tgtgtttggg cgccaccttc tggcgctgca atcataaaga
    10021 ataagtgtgc tggttgcata tccaaacttt gataatctac gcctgcttta gatttaccaa
    10081 acgcaatagc tggtgactta actgcggcca ctttggcatg tggaatggca ataccttcgc
    10141 cgataccagt tgtactttgt gattctcgat tgtgaatcgc ttccttaaat gacgcgacat
    10201 cacttaattt acctgctttg tctaattgat ttactaactc atcaataaca ccatttttgt
    10261 catttgccat taaatccatt gctattgtat cttttgttaa taactctgtt actctcatta
    10321 ttttcactcc ccatcaagta cgctaatcgt aacttgtgat tttatttttt ctatagcgtc
    10381 ccgtgttgct aagtcctcat caaatgccgt ggcagtaccg catgcgactg cttgttggaa
    10441 tgctttttca atcgttaaac ctgaagcaat tccagccacc atgcctgcaa ctgtactatc
    10501 accagagcca actgtattaa ccactttccc ttgtggatta actgctttaa tactgatttc
    10561 tttatcaata taaatagcac catcaccgcc aagcgagaca ataacagatt gcgcaccttt
    10621 atcaactaac aaacgaccat atttaataac atctgtgtct gagttcactg ttgtattaaa
    10681 catcacttct aattcatctt tattaggttt aataaatagt ggatgatatg gtaaaacgct
    10741 ttcagccaat tctttttcag cgtcgactac taatttagca cctgtctgtg ctgtaatttg
    10801 tgcaatttgc gcatacgcat cgcttggaat actacttggt acacttccag caacaataac
    10861 tatatcttcg cttgttgtat ttttaatttg ttgtaacagt tgttcaaatt gtgttgacgt
    10921 tatatgagga cccggtgcat tgatttctgt ttcttgtcct gtttttaatt tcacattaat
    10981 acgtgtatct tcatcaactt caataaaatt cgattgaatt gcactgttat ttaatgtatc
    11041 tataatgaat ttcccaggaa atccacctgc aaatcccaag gcagttgact caacatccaa
    11101 tgtctttaag acgcgcgaga cattaatacc tttcccccca gcgaatttat atgttgctgt
    11161 tgctctgttc aaaccatcaa ttttaaaatc attcgtaaaa atgacatagt caattgaagg
    11221 attgaaagtc actgtataaa tcataaagtc cctcctataa agtgatactt ttgttggtat
    11281 tcttttaacg attcttgatt taatgctttt tcagatgtga tgattgtcgt actttctagc
    11341 aaaggtacac gagcaaaata tactttatta aacttagaat gatctataag tacaaatgat
    11401 tgattggcta atgacattgc tgtttgttta actaatgcct cttgctcatc gggagtagtt
    11461 aatccaagtt caatatctaa tccattcatc ccgataaaag ctttatcgaa acaatatcgt
    11521 cttaatatct ccatagcact agaaccaatc gtagcaagtg tattttcttt aacttgacca
    11581 cctagcataa ttgttttaat acctttttta agtaaagctt ctacatgtgt taaaccatta
    11641 gttaccacaa tgatatcttt cgcttgaata tatttaatta gctccaatgt agatgaacca
    11701 gcatcgataa ataagcattc attatcgttg atttgattag ctgctatttt agcaatcatt
    11761 ttcttttcat caagattcgt tgctaatttt tcagttaaat tcgcctcaac catacgattt
    11821 tcttttaaca ttgcaccacc atgcacacgt tgcagtttcc ctaattgttg tagtttagat
    11881 aaatctcttc gtattgttga agcactgcaa ccagttcgat ctattaattc ttgtaaagtc
    11941 aaaaaatctt tgtgcgaaag ttcttctaat attaactcgt gtcttttttc agtaattatc
    12001 atcatcaccc catctcactt tgtattataa tgagtacgct ttcacttttc aatcataaat
    12061 taccaaaaac aatcacaaac actcaaattg tgacgataaa attacatttt tatgttcaaa
    12121 tactcaatta taatgactgt ttatgatcgt aaaatgctaa ttcaatcaaa aaggagtgtg
    12181 gcagaaatga attttataaa aatttcatct ctgtcacact cctaatcttg ccccatactt
    12241 tttgttgtta ttaatcattc atggataact gctccaattt tgcctttagt tattgtaatc
    12301 aaatcctcaa cagctattgt gatttgcatt gttcgaagcc caccactcac actgatatga
    12361 ctataatttt cacacgattt gtcaacgact gttggaaaca atgttttcat accaacaggc
    12421 gaacacccac cacgaatgta tcccgttaca tttttcaaat tatctaaagg cataagctgc
    12481 aatttcttct ctccaaccaa agcagctgcc tttttcatat ctaaagtttc acttactggg
    12541 ataacaaata caaaatgttc atgttttgta ttttctagaa caagcgtttt aaatactgtt
    12601 ttaacatttg cttgtatgag ttctgcgact tcttgaccag ataaatgttc ttctggtacc
    12661 tcaaagctat gaacttcgta tttaattttt gcacgatcaa gcatacgcat cgcgttcgtt
    12721 ttcttatttt tagccatttc aacacctcat ttatacttct tcagaactat attaacattt
    12781 taaaatacat tacggattgg ttgattttga cataacattt acatcatttt ataagttgaa
    12841 ttcacctttt taaattaaat tattgcaaaa taaaaacagt atatccaatt agaatacact
    12901 gttccatcta ttatttcgtt attatgcctt gatttgaaat acttacatgt tgcttactag
    12961 ttgaattatc attggaatac acataattat atccatgatt tgtcttagaa attctttcta
    13021 aaccataatt atcattgata tgcttatctg cataacgatc agcaataact tggcactctt
    13081 gttcgttaaa agaatttgat ttatttgcat tcacacgatg tttaaaatga ttttgctttt
    13141 tcacattttc attcacttca gatgctttag cagtatgaat attgagaata tccattttct
    13201 ttttataagt atcttcagtt gatacattta tcgcgttaag cgtattaatt gttttatgta
    13261 ccttagcaag cgcatcttca tttgttgcta atattaataa accaattaaa caaacggtta
    13321 atattacagc aacaaagcct aaaaataaat tttttactgt catcccctta cccacatttc
    13381 cttactcatt ttttatcatc ttaacacatt aacgttaatg aattaggtat gtggattgca
    13441 atttaaaaag aatgtaatat ttgtaacatg tttattactc aaaatcattt tttgttacat
    13501 ataacattta aatataaaga gtaaaacatt tagtcaatcg aatcttttta aatgtaaagc
    13561 atcgaatttt cttaataaaa ttcataagaa aaacgatgct aatcattcat aatattacgc
    13621 tttactttat atcaatatac accaaaatac ttatgctaca tattttgttc tttcaatttt
    13681 gctctatgtt gcttttcaat taaaacaata acaacacctg ctaatgaaac acctatagcc
    13741 atataaattg gtgcttcaat gtgtacatca aataacgcac ctgcgattaa aggacctatg
    13801 aaattaccca tactagtgaa tgtcgagttc aatccgcctg caaagccttg cctttctcca
    13861 gcaatattag aaaaataatt tgtaatggct ggtcgtatca tatcaaaacc tatgaagaca
    13921 acaaaactga ttaacattat tgaccaatag tcattagcaa aaactaataa tattaagaca
    13981 acaactgaat ataataatga ccaagctata aatgttaact ctgagaaata cttcataaat
    14041 ttatcgaaga aatagatttg gaaaagtgcc ccaaatatac cgccacccgt aatagcaatc
    14101 gaaatatctt taggtgaata atttaccttg tcagctgtgt atagtgaata caatgtttca
    14161 aatgcagata aaccaaacga taatacaagt gttaaaataa ctggtgtaat aaacactttc
    14221 cagttaattt tcgttagcaa ttgtggctct aacttttgga aaccacttgt cgtagacttt
    14281 ttcggatcgt gaatcaatac aattgacatt ataaatgcta gaatacctaa tgctcctgca
    14341 aagtaaaatg gcatacgatg tgaaacttct gccataaatc caccaatccc tggtcctaaa
    14401 atgaatccag aattgataat cgctgacatg tagccaaagt tttttgcttt ttgatggctt
    14461 ggtgaaatgt cagctattaa acctgtcaca ccaggcatta ccataccagc actcatacca
    14521 ccaatcactc tcgataacat caataccgaa aaattgtggc caactgcaaa cataaattct
    14581 gacactgaaa acaaaattaa tcctatacat ataattaatt tcttccctaa tttgtcagct
    14641 agcgtaccac caaacggcga tataatcatt tgagataacg caaaagcagc aactagtaat
    14701 cctaaatcac taccagttaa tcccaaatct tttaaataaa caggcaagac tggtattact
    14761 aaaccgatac ctaaaaaaat caagaaaata ttaaaatata agacaaaaat ctgtttattc
    14821 atatgctcac ctcttttttc tccatgtcat gcttaaagct gttattcttc ttttccacga
    14881 cagattgcaa ttcatgctaa atattcgtaa aaagtttcta tattgtatta caacattgct
    14941 acatttgaca atattttttc tttgtaatta tcactatttt ccatttaatt gtataattaa
    15001 tgacatatta aaataaaggg gattgatcat atgaaattgc aaaaaagtaa tcatactata
    15061 ctactagtgt tggaaaaagg agaagatatc gttgaatgta taacaacctt tgcagatgac
    15121 caagacttaa catttacatc tgtcagcggc attggtgcat gtgatgacgt tgtattaaaa
    15181 tttttcaatt taacaacgaa acaatacgaa gaaaaacata ttactgagcc actggaattg
    15241 acgagcttat taggtaatat ttctcgtcta gacaatggtc attttgcaca tttgcatgct
    15301 actttcggta ctcaatcata tgaaactttt agcggacatc tcgctaaagc aattgtttct
    15361 gcaactgctg aaatcattct tactgtaact gatttggaca ttcaacgttc attcaaagat
    15421 gcagtcggtt taaatttact tgatcctcaa tagcacttta ctttttagtt gaatcaaatt
    15481 ttattacaag ttatcataag tgcttaatca taaatgcatt ctcgtttatt aatatacttc
    15541 aacgagttaa attaaatttt taattaggaa ttgaatgcgt ttataactat cacatttgaa
    15601 tgaataactt tttcattaga aagtagtgtt tattattgat gcaatctcga tacatcgcta
    15661 gaatttactt tttaatttta tttatcgtct ccctttttga gactaaaatt tttcaattta
    15721 tgactttaaa tttgttttta gcatatatac cttttgagct tagtctatta ttacgattat
    15781 tcaaaccgaa t
//
LOCUS       DAKTIP010000044        15715 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAKTIP010000044 DAKTIP010000000
VERSION     DAKTIP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.044"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..570
                     /locus_tag="O0305_001074"
     CDS             <1..570
                     /locus_tag="O0305_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274224.1"
                     /translation="YLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            637..1572
                     /gene="trxB"
                     /locus_tag="O0305_001075"
     CDS             637..1572
                     /gene="trxB"
                     /locus_tag="O0305_001075"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCY0274225.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            2510..3421
                     /gene="rapZ"
                     /locus_tag="O0305_001076"
     CDS             2510..3421
                     /gene="rapZ"
                     /locus_tag="O0305_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCY0274226.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            3418..4413
                     /locus_tag="O0305_001077"
     CDS             3418..4413
                     /locus_tag="O0305_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCY0274227.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            4523..5467
                     /gene="whiA"
                     /locus_tag="O0305_001078"
     CDS             4523..5467
                     /gene="whiA"
                     /locus_tag="O0305_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCY0274228.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            6041..6628
                     /gene="clpP"
                     /locus_tag="O0305_001079"
     CDS             6041..6628
                     /gene="clpP"
                     /locus_tag="O0305_001079"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCY0274229.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(6871..7773)
                     /locus_tag="O0305_001080"
     CDS             complement(6871..7773)
                     /locus_tag="O0305_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCY0274230.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            8475..9104
                     /locus_tag="O0305_001081"
     CDS             8475..9104
                     /locus_tag="O0305_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCY0274231.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            9864..10877
                     /locus_tag="O0305_001082"
     CDS             9864..10877
                     /locus_tag="O0305_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCY0274232.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            10930..11940
                     /gene="gap"
                     /locus_tag="O0305_001083"
     CDS             10930..11940
                     /gene="gap"
                     /locus_tag="O0305_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCY0274233.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            12079..13269
                     /locus_tag="O0305_001084"
     CDS             12079..13269
                     /locus_tag="O0305_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCY0274234.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            13391..14152
                     /locus_tag="O0305_001085"
     CDS             13391..14152
                     /locus_tag="O0305_001085"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCY0274235.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            14155..15672
                     /locus_tag="O0305_001086"
     CDS             14155..15672
                     /locus_tag="O0305_001086"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCY0274236.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
BASE COUNT         5621 a         2334 c         3025 g         4735 t
ORIGIN      
        1 tatttaggtg aagaagacga aagtcattac tactgggata aattgaagca aatttctaaa
       61 gtggaaattg gacatgcgcc ttgggtaatt gaaaatagca aagaagtttt tgaccaacat
      121 attttgccat tacttcaaag tgatgacagt cattatcgtt tatatggtat ttttttattg
      181 gatcaattaa atggtaaaga aattgtgatg acggaaagta tttggcaggt tttggaaaat
      241 ctaaataatt atgagaaatt gtatttaacg tatttagttc aaggtttaac gctcaataaa
      301 ttagacttca ttcatcgcgg cttattaacg ctttaccata atgaattatt tgtaagtgaa
      361 aatgatgtaa tggttgcatg gattaatcaa ggtgaactca taattgctga aaaagtagat
      421 ttaactgatg ttgagccata tatcggtgcg tttatttatt tgtattttaa aaatcaacct
      481 cgaaacgtta caaagaagca aattacaaca tggttaggca taacacaata taaactgaac
      541 aaaatgattg aatttctctt gagcatatag atttatgaaa agttagattt attatataat
      601 gcgcataatg attaataatg aggaggcgtt aataaaatga ctgaaataga ttttgatata
      661 gcaattatcg gtgcaggtcc agctggtatg actgctgcag tatacgcatc acgtgctaat
      721 ttaaaaacag ttatgattga aagaggtatt ccaggcggtc aaatggctaa tacagaagaa
      781 gtagagaact tccctggttt cgaaatgatt acaggtccag atttatctac aaaaatgttt
      841 gaacacgcta aaaagtttgg tgcagtttat caatatggag atattaaatc tgtagaagat
      901 aaaggcgaat ataaagtgat taactttggt aataaagaat taacagcgaa agcggttatt
      961 attgctacag gtgcagaata caagaaaatt ggtgttccgg gtgaacaaga acttggtgga
     1021 cgcggtgtaa gttattgtgc agtatgtgat ggtgcattct ttaaaaataa acgcctattc
     1081 gttatcggtg gtggtgattc agcagtagaa gagggaacat tcttaactaa atttgctgac
     1141 aaagtaacaa tcgttcaccg tcgtgatgag ttacgtgcac agcgtatttt acaagataga
     1201 gcattcaaaa atgataaaat cgactttatt tggagtcata ctttgaaatc aattaatgaa
     1261 aaagacggca aagtgggttc tgtgacatta acgtctacaa aagatggttc agaagaaaca
     1321 cacgaggctg atggtgtatt catctatatt ggtatgaaac cattaacagc gccatttaaa
     1381 gacttaggta ttacaaatga tgttggttat attgtaacaa aagatgatat gacaacatca
     1441 gtaccaggta tttttgcagc aggagatgtt cgcgacaaag gtttacgcca aattgtcact
     1501 gctactggcg atggtagtat tgcagcgcaa agtgcagcgg aatatattga acatttaaac
     1561 gatcaagctt aattcgaagt cgaattaaga tgttgagctg taaattattt ggatatttat
     1621 tttaatagtg tcatcacagc gttaaaataa tgtcttactt ttaaattaaa gcaaattata
     1681 tagaaaacta gaacttagta cgtatcattt gtgcgtttca atgagttcta gtttttttat
     1741 atgttatatt aaacttataa ctttatggga gtgggacaga aatgataaag agccactaat
     1801 gatttattat gtagtggttc ttaaacatta gccacagcta atgtgtactt aaaaatagga
     1861 atacatgagt aaaactcatg cataagaaat actaatttct atagaaaaag tattacttta
     1921 tcgttgtccc accccaactt gcacattatt gtaagctgac tttccgccag cttctgtgtt
     1981 ggggccccgc caacttgcac attattgtaa gctgactttt cgtcagcttc tgtgttgggg
     2041 ccccgccaac ttgcattgtc tgtagaaatt gggaatccaa tttctctatg ttggggccca
     2101 caccccaact cgcattgcct gtagaatttc ttttcgaaat tctctgtgtt ggggcccaca
     2161 ccccaactcg cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccacacc
     2221 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccctgacta
     2281 gagttgaaaa aagcttgttg caagcgcatt ttcattcagt caactactag caatataata
     2341 ttatagaccc taggacattg atttatgtcc caagctcctt ttaaatgatg tatattttta
     2401 gaaatttaat ctagacatag ttggaaataa atataaaaca tcgttgctta attttgtcat
     2461 agaacattta aattaacatc atgaaattcg ttttggcggt gaaaaaataa tggataataa
     2521 tgaaaaagaa aaaagtaaaa gtgaactatt agttgtaaca ggtttatctg gcgcaggtaa
     2581 atctttggtt attcaatgtt tagaagacat gggatatttt tgtgtagata atctaccacc
     2641 agtgttattg cctaaatttg tagagttgat ggaacaagga aatccatcct taagaaaagt
     2701 ggcaattgca attgatttaa gaggtaagga actatttaat tcattagttg cagtagtgga
     2761 taaagtcaaa agtgaaagtg acgtcatcat tgatgttatg tttttagaag caagtactga
     2821 aaaattaatt tcaagatata aggaaacgcg tcgtgcacat cctttgatgg aacaaggtaa
     2881 aagatcgtta atcaatgcaa ttaatgatga gcgagagcat ttgtctcaaa ttagaagtat
     2941 agctaatttt gttatagata ctacaaagtt atcacctaaa gaattaaaag aacgcattcg
     3001 tcgatactat gaagatgaag agtttgaaac ttttacaatt aatgtcacaa gtttcggttt
     3061 taaacatggg attcagatgg atgcagattt agtatttgat gtacgatttt taccaaatcc
     3121 atattatgta gtagatttaa gacctttaac aggattagat aaagacgttt ataattatgt
     3181 tatgaaatgg aaagagacgg agattttctt tgaaaaatta actgatttgt tagattttat
     3241 gatacccggg tataaaaaag aagggaaatc tcaattagta attgccatcg gttgtacggg
     3301 tggacaacat cgatctgtag cattagcaga acgactaggt aattatctaa atgaagtatt
     3361 tgaatataat gtttatgtgc atcataggga cgcacatatt gaaagtggcg agaaaaaatg
     3421 agacaaataa aagttgtact tatcggtggt ggcactggct tatcagttat ggctagggga
     3481 ttaagagaat tcccaattga tattacggcg attgtaacag ttgctgataa tggtgggagt
     3541 acagggaaaa tcagagatga aatggatata ccagcaccag gagacatcag aaatgtgatt
     3601 gcagctttaa gtgattctga gtcagtttta agccaacttt ttcagtatcg ctttgaagaa
     3661 aatcaaatta gcggtcactc attaggtaat ttattaatcg caggtatgac taatattacg
     3721 aatgatttcg gacatgccat taaagcatta agtaaaattt taaatattaa aggtagagtc
     3781 attccatcta caaatacaag tgtgcaatta aatgctgtta tggaagatgg agaaattgtt
     3841 tttggagaaa caaatattcc taaaaaacat aaaaaaattg atcgtgtgtt tttagaacct
     3901 aacgatgtgc aaccaatgga agaagcaatc gatgctttaa gggaagcaga tttaatcgtt
     3961 cttggaccag ggtcattata tacgagcgtt atttctaact tatgtgtgaa tggtatttca
     4021 gatgcgttaa ttcattctga tgcgcctaag ctatatgttt ctaatgtgat gacgcaacct
     4081 ggggaaacag atggttatag cgtgaaagat catatcgatg cgattcatag acaagctgga
     4141 caaccgttta ttgattatgt catttgtagt acacaaactt tcaatgctca agttttgaaa
     4201 aaatatgaag aaaaacattc taaaccagtt gaagttaata aggctgaact tgaaaaagaa
     4261 agcataaatg taaaaacatc ttcaaattta gttgaaattt ctgaaaatca tttagtaaga
     4321 cataatacta aagtgttatc gacaatgatt tatgacatag ctttagaatt aattagtact
     4381 attcctttcg taccaagtga taaacgtaaa taatatagaa cgtaatcata ttatgatatg
     4441 ataatagagc tgtgaaaaaa atgaaaatag acagtggttc taaggtgaat catgttttaa
     4501 ataagaaagg aatgactgta cgatgagctt tgcatcagaa atgaaaaatg aattaactag
     4561 aatagacgtc gatgaaatga atgcaaaagc agagctcagt gcactgattc gaatgaatgg
     4621 tgcacttagt ctttcaaatc aacaatttgt tataaatgtt caaacggaaa atgcaacaac
     4681 ggcaagacgt atttattcgt tgattaaacg tgtctttaat gtggaagttg aaatattagt
     4741 ccgtaaaaaa atgaaactta aaaaaaataa tatttatatt tgtcgtacaa agatgaaagc
     4801 gaaagaaatt cttgatgaat taggaatttt aaaagacggc atttttacgc atgaaattga
     4861 tcattcaatg attcaagatg acgaaatgag acgcagttac ttgagaggag cttttctggc
     4921 aggtggctca gtgaataacc ctgaaacatc ttcgtaccat ttggaaattt tttctcaaaa
     4981 tgagagtcat gcagaaggct taacgaaact aatgaatagt tatgagttga atgccaaaca
     5041 tttagagcga aaaaaaggaa gtattacgta tttaaaagaa gcggaaaaga tttcggattt
     5101 tcttagtttg ataggtggct atcaagcgtt attaaaattt gaagacgtac gtattgtaag
     5161 agatatgcgt aattctgtta accgactcgt taattgtgaa acggccaatc taaataaaac
     5221 agttagtgct gcgatgaaac aagttgagag cattaaattg attgataaag aaattggtat
     5281 tgaaaattta ccagacaggt tgagagagat tgctagaatt cgagtagaac atcaagaaat
     5341 ttcgttgaaa gagcttggag aaatggtatc aactggtcca atttcaaaat caggtgtaaa
     5401 tcaccgatta agaaaactga atgatttagc cgataagatt agaaatggtg aacaaataga
     5461 attataagta agaaggtgtt tttggtagtg aattatcaaa aacacttttt ttatgattaa
     5521 aaagtgttta atgaataata gtcgactaac tattaaattg agctggaaag tttaatgaag
     5581 gaaattcaat aaccaagtga tgcctaattt tctcattaaa tagcatcata tgtatcaatt
     5641 tggtcataag aaaatatgaa tgtgataatt aattgagtcc ctgaaagtcc ctgatcatag
     5701 acaaaaacaa aatcagcata aataagaatc ccgacattgc gggattctct gtattgaaaa
     5761 gtattgtatt ttattaaaac agcctccttg aagggaattg aacccctatc ttaagaaccg
     5821 gaatcttacg tgttatccat tacaccacaa ggagcaaata tgtaatcata tctctgtact
     5881 atgagaagta aaataagtac aatctaaaat agatattatc agtttacaaa ggaaagagaa
     5941 aagcgtcaaa caatgtaact atttaaagtc aaagtgtttg accaaatttg acttaatatg
     6001 taaaataatg agtaacagtt attacaagga ggaaatatag atgaatttaa ttcctacagt
     6061 tattgaaaca acaaaccgcg gtgaacgtgc atatgatata tactcacgtt tattaaaaga
     6121 ccgtattatt atgttaggtt cacaaattga tgacaacgta gcaaattcaa tcgtatcaca
     6181 gttattattc ttacaagcgc aagactcaga gaaagatatt tatttataca ttaattcacc
     6241 aggtggaagt gtaacagctg gttttgcgat ttatgataca attcaacaca ttaaacctga
     6301 tgttcaaaca atttgtatcg gtatggctgc atcaatggga tcattcttat tagcagctgg
     6361 tgcaaaaggt aaacgtttcg cgttaccaaa tgcagaagta atgattcacc aaccattagg
     6421 tggtgctcaa ggacaagcaa ctgaaatcga aattgctgca aatcacattt taaaaacacg
     6481 tgaaaaatta aaccgcattt tatcagagcg tactggtcaa agtattgaaa aaatacaaaa
     6541 agacacagat cgtgataact tcttaactgc agaagaagct aaagaatatg gcttaattga
     6601 tgaagtgatg gtacctgaaa caaaataatt caaagtaaag agtagactaa gctgtctgct
     6661 ctttttgtat gagtaaacca aggtgtcaat aatttgttta ctatactttg agcggaaata
     6721 tgattgaatg aagctagttg aaccgtaact atatgaaatg ttcccttcaa agtagacatt
     6781 gaaaggaaca tttcaatcct ttgtttgtaa gtcgctctag acattacatt tagtacatat
     6841 gttgtttcta atgctcatta atggtattga ttattcttta attaaatctt caagtgccat
     6901 ttttaaatta ctatatttaa attggaatcc caatgcttga attttattag gtaatacttt
     6961 ttgagtatcc aatactactg ttgacatttg accaagtatg agacgcattg caagacttgg
     7021 tgcccaagtt tcatgaggct tatgcatagc tcttgctaaa gtgtagccaa ataaattttg
     7081 acgttcaggt ataggtgcag ttaaattaaa cggaccacta gctgactcgt tatttattaa
     7141 aaataaaata gcttgaatta aatcattgat atgaatccat gaataccatt gttgaccaga
     7201 acctaattta ccaccaatgt aatattcgta tggtagtttc attgtttgta acgcaccgcc
     7261 ttcattcgat aaaattatac cgaaacgacc gatgacaact cgtgtaccta attgttcaaa
     7321 ttgttgtgcg aaacgttccc attgatacac aatatctgat aagaaatcaa aaggtaaagt
     7381 tttataaact tctgtgtaac tcataaataa atcaggagga tagtaaccag tggcactagc
     7441 attaaataaa gctttaggtg ctttattacg tgatttaaac aattcatata aagcttgcgt
     7501 agattgaatt ctacttagca ttagcgtttg tttatattcc ggtgtccatc gtttattcaa
     7561 tgtagcacct gctaagttga tgaccacatc gatattttga ggaactttgt gttcccaccc
     7621 agatttagcc cagttgacat atgaaatttt cttatcattt gaaatttggt cgtgtcgcgt
     7681 taatatcgtg atatgtgaat ctgatttttt aatttcatta actaattgag atccaaccat
     7741 accagtccca ccagtaatta agtattgttt cattatcatt caccccatgt aattttgtat
     7801 ttagttacta tttaataatc attattgttg ttcaaaggtt atacattatt tagacaataa
     7861 tatgtcaata actttttttg aattgtattt taataaaaat gatataagtt ataataaagg
     7921 tgtaccttcg ataacgaata aacatctctt aaaagtatgt gtaaaacgct gcatgataca
     7981 aacgaaggta aaaatttgac tccctttagt agtggacccg tacgttaatc gtgcgggtcg
     8041 tttttattta tttcttattc ccattataca tcaatttaaa gcattaattt tttaaacaaa
     8101 tttaagaata catagtaata taacaatcta aacataaaaa cttttaacac aacacttaaa
     8161 ccaatgcttt aattttcaat acgtagctat aatttgttgt aaaatcaaaa aggttaaaat
     8221 gttaattttc aaaaaaggct caaaatatgt ttgatttagt tattaaatgt taagatatat
     8281 aagactacta tttctttgta aaaatgaatc cgatttacga gtgagtaata gtgaaggcag
     8341 ttttaagttg aagaaggcaa aaagagtaaa tgtttattaa tatttgtaga aactaggtaa
     8401 gcaaattagt tgtgaaaatg ttaatggttg cgtgataatt tctatattta aattagtttg
     8461 aagtgaggga gagtatgtcg aatcaaaatt acgactacaa taaaaatgaa gatggaagta
     8521 agaagaaaat gagtacaaca gcgaaagtag ttagcattgc gacggtattg ctattactcg
     8581 gaggattagt atttgcaatt tttgcatatg tagatcattc gaataaagct aaagaacgta
     8641 tgttgaacga acaaaagcag gaacaaaaag aaaagcgtca aaaagaaaat gcagaaaaag
     8701 agagaaagaa aaagcaacaa gaggaaaaag agcagaatga gctagattca caagcaaacc
     8761 aatatcagca attgccacag cagaatcaat atcaatatgt gccacctcag caacaagcac
     8821 ctacaaagca acgtcctgct aaagaagaga atgatgataa agcatcaaag gatgagtcga
     8881 aagataagga tgacaaagca tctcaagata aatcagatga taatcagaag aaaactgatg
     8941 ataataaaca accagctcag cctaaaccac agccgcaaca accaacacca aagccaaata
     9001 ataatcaaca aaacaatcaa tcaaatcagc aagcaaaacc acaagcacca caacaaaata
     9061 gccaatcaac aacaaataaa caaaataatg ctaatgataa gtagtattta gtcaaacaaa
     9121 aatgaaccag tatgacagac aacacaatta attaggttgt ctcgaatatt ggttcttatt
     9181 tttataattg ttaattaggg gagagatgat acttaaatag ttagttattt attttacgga
     9241 tagtgaaatt tattttgagt gaggtgggac agaaatgata ttttcgcaaa atttatttcg
     9301 tcgtcccacc ccaacttcca ttgcctgtag aaattggaaa tccaatttct ctatgttggg
     9361 gccccacccc aacttgcatt gcctgtagaa attgggagga gccaatttca ctatgttggg
     9421 gccccacccc aactcgcatt gcctgtagaa tttcatttcg aaattctcta tgttggggcc
     9481 cctgacttta attgaaaaaa gcttgttaca agtgcatttt cgttcggtta actactacta
     9541 atgtgacttt ttggattcta gagcattgat ttatgtccta gtctcaaata ataagcaaat
     9601 gattatcgag tcagtataag ggtatacatt tgactacagc gaataaaata aaacgttttg
     9661 ttgaataaca aatcgaaaat atattgcaag cgctttatca aattatttag aaaatttaag
     9721 ttttatgctt gcaatttttg aaatagaata gtactattgc aagtgtaaag aggttaattt
     9781 ttgtcccacg cgggacttaa aaaggcaacc actggttgtg acatatcctt atttacattt
     9841 ataaatataa ggaggaggta gtagtgaaag acttattgca agcacagcaa aagcttatac
     9901 cggatctcat agataaaatg tataaacgtt tttctattct tactactatc tcaaaaaatc
     9961 agcctgtcgg acgtcgaagt ttaagcgaac atatggatat gactgaacgt gtactgcgtt
    10021 ctgaaacaga tatgcttaag aaacaagatt tgataaaagt taagcctacc ggaatggaaa
    10081 ttacagctga aggtgagcaa ctgatttcgc aattgaaagg ttactttgat atctatgcag
    10141 atgataatcg tctgtcagaa ggtattaaga ataaatttca aattaaggaa gttcatgttg
    10201 ttcctggtga tgctgataat agtcaatctg ttaaaacaga attaggtaga caagcaggtc
    10261 aattacttga aggcatatta caagaagacg cgatagttgc tgtaactggc ggatccacga
    10321 tggcatgtgt tagtgaagca attcatttat taccatataa tgtattcttc gtaccagcca
    10381 gaggtggact aggcgaaaat gttgtctttc aggcaaacac aattgcagcc agtatggcac
    10441 aacaagctgg cggttattat acgacgatgt atgtacctga taatgtcagt gaaacaacat
    10501 ataatacatt gttgttagag ccatcagtca taaacacttt agacaaaatt aaacaagcaa
    10561 acgttatatt acacggcatt ggtgatgcgc tgaagatggc gcatcgacgt caatcacctg
    10621 aaaaggtcat tgaacaactt caacatcatc aagctgtcgg agaggcattt ggttattatt
    10681 ttgatacaca aggtcaaatt gtccataagg ttaaaacaat tggacttcaa ttagaagacc
    10741 ttgaatcaaa agactttatt tttgcagttg caggaggcaa atcgaaaggt gaagcaatta
    10801 aagcatactt gacgattgca cccaagaata cagtgttaat cactgatgaa gccgcagcaa
    10861 agataatact tgaataagag ataaaaagtt taatactttt taaatatcat tttaaaggag
    10921 gccattataa tggcagtaaa agtagcaatt aatggttttg gtagaattgg tcgtttagca
    10981 ttcagaagaa ttcaagaagt agaaggtctt gaagttgtag cagtaaacga cttaacagat
    11041 gacgacatgt tagcgcattt attaaaatat gacactatgc aaggtcgttt cacaggtgaa
    11101 gtagaggtag ttgatggtgg tttccgcgta aatggtaaag aagttaaatc attcagtgaa
    11161 ccagatgcaa gcaaattacc ttggaaagac ttaaatatcg atgtagtatt agaatgtact
    11221 ggtttctaca ctgataaaga taaagcacaa gctcatattg aagcaggcgc taaaaaagta
    11281 ttaatctcag caccagctac tggtgactta aaaacaatcg tattcaacac taaccaccaa
    11341 gagttagacg gttctgaaac agttgtttca ggtgcttcat gtactacaaa ctcattagca
    11401 ccagttgcta aagttttaaa cgatgacttt ggtttagttg aaggtttaat gactacaatt
    11461 cacgcttaca caggtgatca aaatacacaa gacgcacctc acagaaaagg tgacaaacgt
    11521 cgtgctcgtg cagcggcaga aaacatcatc cctaactcaa caggtgctgc taaagctatc
    11581 ggtaaagtta ttcctgaaat cgatggtaaa ttagatggtg gtgcacaacg tgttcctgta
    11641 gctacaggtt cattaactga attaacagta gtattagaaa aacaagacgt aacagttgaa
    11701 caagttaacg aagctatgaa aaatgcttca aacgaatcat tcggttacac tgaagacgaa
    11761 atcgtttctt cagacgttgt aggtatgact tacggttcat tattcgacgc tacacaaact
    11821 cgtgtaatgt cagttggcga ccgtcaatta gttaaagttg cagcttggta tgataacgaa
    11881 atgtcatata ctgcacaatt agttcgtaca ttagcatact tagctgaact ttctaaataa
    11941 ttttagtata gtttttattc aaatacgcta gtgctcagaa ctatttagca ttaattaaag
    12001 cttatgagta agcggggagc acaaacgctt ctccgcttat ttttatataa aatttcctaa
    12061 ttacaaggag gaaacaccat ggctaaaaaa attgtttctg atttagatct taaaggtaaa
    12121 acagtcctag tacgtgctga ttttaacgta cctttaaaag acggtgaaat tactaatgac
    12181 aaccgtatcg ttcaagcttt acctacaatt caatacatca tcgaacaagg tggtaaaatc
    12241 gtactatttt cacatttagg taaagtgaaa gaagaaagtg ataaagcaaa attaacttta
    12301 cgtccagttg ctgaagactt atctaagaaa ttagataaag aagttgtttt cgtaccagaa
    12361 acacgcggcg aaaaacttga agctgctatt aaagacctta aagaaggcga cgtattatta
    12421 gttgaaaata cacgttatga agatttagac ggtaaaaaag aatctaaaaa tgatccagaa
    12481 ttaggtaaat actgggcatc tttaggtgat gtgtttgtaa atgatgcttt tggtactgcg
    12541 catcgtgagc atgcatctaa tgttggtatt tctacacatt tagaaactgc agctggattc
    12601 ttaatggata aagaaattaa gtttattggc ggcgtagtta acgatccaca taaaccagtt
    12661 gttgctattt taggtggagc aaaagtatct gacaaaatta atgtcatcaa aaacttagtt
    12721 aacatagctg ataaaattat catcggcgga ggtatggctt atactttctt aaaagcgcaa
    12781 ggtaaagaaa ttggtatttc attattagaa gaagataaaa tcgacttcgc aaaagattta
    12841 ttagaaaaac atggtgataa aattgtatta ccagtagaca ctaaagttgc taaagaattt
    12901 tctaatgatg ccaaaatcac tgtagtacca tctgattcaa ttccagcaga ccaagaaggt
    12961 atggatattg gaccaaacac tgtaaaatta tttgcagatg aattagaagg tgcgcacact
    13021 gttgtatgga atggacctat gggtgtattc gagttcagta actttgcaca aggtacaatt
    13081 ggtgtatgta aagcaattgc aaaccttaaa gatgcaatta cgattatcgg tggcggtgat
    13141 tcagctgcag cagcaatctc tttaggtttt gaaaatgact tcactcatat ttcaactggt
    13201 ggcggcgcgt cattagagta cctagaaggt aaagaattgc ctggtatcaa agcaatcaat
    13261 aataaataat aaagtgatag tttaaagtga tgtggcatgt ttgtttaaca ttgttacggg
    13321 aaaacagtca caagatgaca tcgtgtttca tcacttttca aaaatattta caaaacaagg
    13381 agtgtcttta atgagaacac caattatagc tggtaactgg aaaatgaaca aaacagtaca
    13441 agaagcaaaa gacttcgtca atgcattacc aacattacca gattcaaaag aagtagaatc
    13501 agtaatttgt gcaccagcaa ttcaattaga tgcattaact actgcagtta aagaaggaaa
    13561 agcacaaggt ttagaaatcg gtgctcaaaa tacgtatttc gaagataatg gtgcgttcac
    13621 aggtgaaacg tctccagttg cattagcaga tttaggcgtt aaatacgttg ttatcggtca
    13681 ttctgaacgt cgtgaattat tccacgaaac agatgaagaa attaacaaaa aagcgcacgc
    13741 tattttcaaa catggaatga ctccaattat atgtgttggt gaaacagacg aagagcgtga
    13801 aagtggtaaa gctaacgatg ttgtaggtga gcaagttaag aaagctgttg caggtttatc
    13861 tgaagatcaa cttaaatcag ttgtaattgc ttatgaacca atctgggcaa tcggaactgg
    13921 taaatcatca acatctgaag atgcaaatga aatgtgtgca tttgtacgtc aaactattgc
    13981 tgacttatca agcaaagaag tatcagaagc aactcgtatt caatatggtg gtagtgttaa
    14041 acctaacaac attaaagaat acatggcaca aactgatatt gatggggcat tagtaggtgg
    14101 cgcatcactt aaagttgaag atttcgtaca attgttagaa ggtgcaaaat aatcatggct
    14161 aagaaaccaa ctgcgttaat tattttagat ggttttgcga accgcgaaag cgaacatggt
    14221 aatgcggtaa aattagcaaa caagcctaat tttgatcgtt attacaacaa atatccaacg
    14281 actcaaatcg aagcgagtgg cttagatgtt ggactacctg aaggacaaat gggtaactca
    14341 gaagttggtc atatgaatat cggtgcagga cgtatcgttt atcaaagttt aactcgaatc
    14401 aataaatcaa ttgaagacgg tgatttcttt gaaaatgatg ttttaaataa tgcaattgca
    14461 cacgtgaatt cacatgattc agcgttacac atctttggtt tattgtctga cggtggtgta
    14521 cacagtcatt acaaacattt atttgctttg ttagaacttg ctaaaaaaca aggtgttgaa
    14581 aaagtttacg tacacgcatt tttagatggc cgtgacgtag atcaaaaatc cgctttgaaa
    14641 tacatcgaag agactgaagc taaattcaat gaattaggca ttggtcaatt tgcatctgtg
    14701 tctggtcgtt attatgcaat ggatcgtgac aaacgttggg aacgtgaaga aaaagcttac
    14761 aatgctattc gtaattttga tgccccaact tatgcaactg ccaaagaagg tgtagaagca
    14821 agctataatg agggcttaac tgacgaattc gtagtaccat tcatcgttga gaatcaaaat
    14881 gacggtgtta atgatggaga tgcagtgatc ttctataatt tccgacctga tagagcagcg
    14941 caattatcgg aaatttttgc gaacagagca ttcgaaggct ttaaagttga acaagttaaa
    15001 gacttattct atgcaacatt cactaagtat aatgacaata tcgatgcggc tatcgtcttc
    15061 gaaaaagttg atttaaataa tacaattggt gaaattgcac aaaataacaa tttaactcaa
    15121 ttacgtattg cagaaactga aaaataccct cacgttactt actttatgag tggtggacgt
    15181 aacgaggaat ttaaaggtga acgccgtcgt ttaattgatt cacctaaagt tgcaacgtat
    15241 gacttgaaac cagaaatgag tgcttatgaa gttaaagatg cattattaga agagttaaat
    15301 aaaggtgact tggacttaat tattttaaac tttgctaacc ctgatatggt tggacatagt
    15361 ggtatgcttg agccgacaat caaagcaatc gaagcggttg atgaatgttt aggagaagtg
    15421 gttgataaga ttttagacat ggacggttat gcaattatta ctgctgacca tggtaactct
    15481 gatcaagtat tgacggatga tgatcaacca atgactacgc atacaacgaa cccagtacca
    15541 gtgattgtaa caaaagaagg cgttacactt agagaaactg gtcgcttagg tgacttagca
    15601 cctacattat tagatttatt aaatgtagaa caacctgaag atatgacagg tgaatcttta
    15661 attaaacact aatattgtaa aagatgttaa gtaaacgctt aatgacactt atttt
//
LOCUS       DAKTIP010000045        15622 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAKTIP010000045 DAKTIP010000000
VERSION     DAKTIP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.045"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(107..967)
                     /gene="folD"
                     /locus_tag="O0305_001087"
     CDS             complement(107..967)
                     /gene="folD"
                     /locus_tag="O0305_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCY0274237.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1168..1650
                     /gene="purE"
                     /locus_tag="O0305_001088"
     CDS             1168..1650
                     /gene="purE"
                     /locus_tag="O0305_001088"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCY0274238.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            1637..2761
                     /gene="purK"
                     /locus_tag="O0305_001089"
     CDS             1637..2761
                     /gene="purK"
                     /locus_tag="O0305_001089"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCY0274239.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            2765..3469
                     /locus_tag="O0305_001090"
     CDS             2765..3469
                     /locus_tag="O0305_001090"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCY0274240.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3469..3732
                     /gene="purS"
                     /locus_tag="O0305_001091"
     CDS             3469..3732
                     /gene="purS"
                     /locus_tag="O0305_001091"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCY0274241.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            3734..4405
                     /gene="purQ"
                     /locus_tag="O0305_001092"
     CDS             3734..4405
                     /gene="purQ"
                     /locus_tag="O0305_001092"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCY0274242.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4398..6587
                     /gene="purL"
                     /locus_tag="O0305_001093"
     CDS             4398..6587
                     /gene="purL"
                     /locus_tag="O0305_001093"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCY0274243.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6566..8050
                     /locus_tag="O0305_001094"
     CDS             6566..8050
                     /locus_tag="O0305_001094"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCY0274244.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8043..9071
                     /locus_tag="O0305_001095"
     CDS             8043..9071
                     /locus_tag="O0305_001095"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCY0274245.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDVSRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9074..9640
                     /gene="purN"
                     /locus_tag="O0305_001096"
     CDS             9074..9640
                     /gene="purN"
                     /locus_tag="O0305_001096"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCY0274246.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVTLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            9655..11133
                     /gene="purH"
                     /locus_tag="O0305_001097"
     CDS             9655..11133
                     /gene="purH"
                     /locus_tag="O0305_001097"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCY0274247.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPATVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPSGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11155..12402
                     /locus_tag="O0305_001098"
     CDS             11155..12402
                     /locus_tag="O0305_001098"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCY0274248.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQAILDFAKRQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(12667..13473)
                     /locus_tag="O0305_001099"
     CDS             complement(12667..13473)
                     /locus_tag="O0305_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0274249.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITMAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13466..14866)
                     /locus_tag="O0305_001100"
     CDS             complement(13466..14866)
                     /locus_tag="O0305_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274250.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQLP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(14881..15456)
                     /locus_tag="O0305_001101"
     CDS             complement(14881..15456)
                     /locus_tag="O0305_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0274251.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15533..>15622)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5452 a         2338 c         3005 g         4827 t
ORIGIN      
        1 gttgactaga attgaaaaaa gcttgttaca agcgaatttt cgttcagtca actactgcca
       61 ttataacttt gtagagcata gaacattgat ttatgtctca ggctctttac gaatcaatac
      121 ctcgacgcat tttttctgca agcaaagtat tatttaatac catagtaatt gttaatggac
      181 caacgccacc aggaactggt gtaatagctc cagcaatttc tttaaccgca tcataatcaa
      241 cgtcaccttt taatttgcca ttttcatctg gcgtattgcc aacatcgata attactgctc
      301 cttctttgac cacatctttt gttactaaac caggcttacc aactgcactg acaatgacat
      361 cagcatcttt taaatatgat gccatatctt ttgaacgaga atgtaagatt gttactgatg
      421 catttttttg aagtagtaac ttagaaactg gttgtccgac aatatgactt cgtccaatta
      481 caactgcatt tttaccttct aaatcaatat cagcatgttt taatatttcc atgatgccga
      541 gcggtgtgca aggtacaaaa gtttgttcat cgatatataa tttccctata tttattggat
      601 gaaaaccgtc cacatctttt tcaggattga ttgcttctaa tattttctgt tcgctaactt
      661 gttttggtaa tggtacttgt accaaaatac cacttacaga atcatcatta tttagtctat
      721 ttagttcgtt taatacttct tcttcagtag ctgtttcttc caaatgtacg atttctgaaa
      781 tcataccaat tttttcagct gctttctttt ttgatctaac ataactttga ctagcgccat
      841 cattaccaac taatataacg gataatttag gtgtaaaacc cttttctttt agcgcttcaa
      901 cttgatcttg taacccctgt ctgtagtctt tggcaatttg tttaccatct aaaattttag
      961 caaccataaa aacttctcct cctattgtca atatcctaac tttgttagtt tataaaacta
     1021 atttacatag caaaagacgt aaattcaaac aaaaaatcaa aataaagttc gatttttgat
     1081 tgaaaaagca gaaattgctt gttatgctat atctataata tacaactaaa gtaattcaca
     1141 attcaaactt ttgaaagggt gtacaaagtg aaagtagcag tcattatggg cagttcttcc
     1201 gattggaaaa ttatgcaaga gagttgtaac atgttggatt attttgaaat tccgtacgaa
     1261 aaacaagtag tatccgcaca tcgtacgcca aaaatgatgg ttcaatttgc ttctgaagcg
     1321 agagaaagag gtataaacat tatcattgca ggcgctggcg gtgcggcaca tttaccaggt
     1381 atggttgcat cattaacgac gctaccagtt attggagtgc cgattgaaac aaaaagttta
     1441 aagggtatag attctttatt atcaattgtt caaatgccag gaggtattcc ggttgcaacg
     1501 actgcaattg gtgcagcagg tgctaaaaac gcaggtatac ttgcagcaag aatgttaagt
     1561 attcaaaatc cttctttagt tgaaaaacta aatcagtatg aatcttcgtt aattcaaaaa
     1621 gtggaggaca tgcaaaatga acttcaataa attaaagttt ggtgcgacta ttggcattat
     1681 tggtggtggt cagcttggaa agatgatggc acaatcagct caaaaaatgg gttataaagt
     1741 ggttgtattg gatccttctg aagattgtcc atgtagatac gttgcacacg aatttataca
     1801 agccaagtat gacgatgaaa aggcactcaa tcaattagga caaaaatgtg atgtgattac
     1861 ttatgaattt gaaaacattt cagcccaaca attaaaacta ttatgtgaaa agtacaatat
     1921 tccgcaaggt taccaagcta tacagttatt acaagatcgc ttaactgaaa aagaaacatt
     1981 aaaaagtgct ggtaccaaag ttgtcccgtt catttcagta aaagaatcta cagatattga
     2041 caaagcaatt gaaacattag gatatccttt tattgtaaaa actagatttg gtggctacga
     2101 tggcaaaggt caagttttaa ttaacaacga aaaagactta caagaaggtt ttaaattaat
     2161 tgaaactagt gaatgcgtag ctgaaaaata tttgaatatc aagaaagaag tatctcttac
     2221 tgttacaaga ggaaacaaca atcaaatcac ttttttccca ttacaagaaa atgagcatag
     2281 aaatcaaata cttttcaaaa caattgttcc agcgagaata gataaaacag ctgaggcgaa
     2341 agagcaagtt aataaaatta tccaatcgat tcatttcatt ggaacattta cagttgaatt
     2401 ttttatagat agtaacaacc aattgtatgt gaacgagata gcaccaaggc ctcacaattc
     2461 cggacattat tcaattgaag catgtgatta ttcacaattt gatactcata ttttagcagt
     2521 taccggacaa tcattaccaa attcaattga attattaaag ccagcagtca tgatgaactt
     2581 actaggtaaa gatttagatt tattggaaaa tgaatttaat gaacatccag agtggcactt
     2641 acatatttat ggtaagtctg agcgtaaaga tagcagaaaa atggggcata tgactgtact
     2701 aacgaatgat gtaaaccaaa ctgaacaaga tatgtacgct aaatttgagg ggagtaatta
     2761 aagcatgaca ttattatatg aaggaaaagc gaagcgcatt ttctcaacaa atcaagaaaa
     2821 tgaattaaga gttgaatata aagatgaagt tactgctgga aacggggcta agaaagacac
     2881 aatggcaggt aaggggcgat taaataatca aattacttct attatattta aatatttaca
     2941 agaaaatgga atagaaagtc actttattaa acaattatct gaaacagaac aattagttaa
     3001 gcctgtgaaa ataattccat tagaagtagt tgttcgtaat attgctagtg gatctattac
     3061 aaagcgttta ggttttgaaa atggtgaagt ttttagagaa ccacttgtag aatttttcta
     3121 taaaaatgat gcgttaaatg atccgttgat aacggatgac catgttaaat tgctcaatat
     3181 agcatcagat gaagatattg aaatactaaa atccaaagca ttaaagatta ataatgtgtt
     3241 gaaacaatta atggatgcta tgaatttaaa attagtagat tttaaaatcg aatttggaaa
     3301 gactgagact ggtcaaattt tgttagcgga tgaaatatct ccagatacat gtcgaatttg
     3361 ggataaagct accaatgcaa actttgataa agatgtatat agaaataaca ctggatcact
     3421 gattgaaaca tatcaaatat ttttaaacaa attggaggat ttaaaataat gaaaacaatt
     3481 gaactacata tcacattaca accacaagta ttagatacgc aaggacaaac gcttactcga
     3541 gctgtacatg acttaggtta tgcacaagtg aatgatattc gtgtaggaaa agtattatat
     3601 atgacagtgg atgaggttag tgatgaaaag gtacacaaca ttattacaac tctaagtgaa
     3661 aaattgtttg caaatacagt gattgaagaa tatagctata aagtgttaga tgatgaaaag
     3721 gagaatgcat aaaatgaaat ttgcggttct tgtttttcca ggttcgaatt gtgatagaga
     3781 catgtttaat gctgctatta aaagtggtgt tgaagcggaa tatgtagatt atagagaaac
     3841 atcactaagt ggatttgatg gcgtacttat tcctggtgga ttttcattcg gggattactt
     3901 aagatctggg gcaatggcta gtgtagcgcc gattatttcg gaagttaaac gtcttgcagc
     3961 tgaaggtaag ccagtattag gtgtttgtaa tgggtttcaa attttaactg aaataggctt
     4021 attacctggt gcattattgc ataacgattc acatttattt attagtagaa atgaagagtt
     4081 agaaatagtg aataatcaaa cggcatttac aaatctttat gaacaaggtg aaaaagttat
     4141 atatcctgta gctcacggtg aaggtcatta ttattgtact gatgaaatat atcaacaatt
     4201 aaaagctaac aatcaaatta ttctgaaata tgtgaataat ccgaacggtt catatgatga
     4261 tattgcagga attgttaacg aaaaaggcaa tgtatgtggc atgatgccac atcctgaaag
     4321 agctttagaa acgttgttag gtactgatag tggtgtgaaa ttatttgaag cgatggtaaa
     4381 aagttggagg gaacaacatg tctaaattta tcgaaccaag cgttgaagaa attaaacttg
     4441 aaaaagtata tcaagatatg ggattaagtg atcaagaata tgaaaaagtt tgcgatattt
     4501 taggcagaca acctaacttt acagaaacag gtatcttttc tgttatgtgg agtgaacatt
     4561 gctcttataa acattctaaa ccgtttttaa agcaatttcc tacgtcaggt gaccatgtgc
     4621 ttatggggcc tggtgaaggt gcaggggtag tcgatatagg tgataatcaa gccgtagtat
     4681 ttaaagtaga gtctcacaat catccatcag caattgaacc atatcaaggg gctgctacag
     4741 gcgttggtgg aatcattcgt gacattgtct ctattggggc tagacctatt aatttgttaa
     4801 acagtcttag atttggagaa ttagataata aacaaaacca aagattactt aaaggtgttg
     4861 taaagggtat cggaggttat ggtaactgca ttggtattcc aacaactgct ggtgaaatcg
     4921 aatttgatga acgttatgat ggcaatccac ttgttaatgc aatgtgtgtt ggtgttatca
     4981 atcacgacat gattcaaaaa ggcacagcaa aaggtgtagg taattcggtc atttatgttg
     5041 gtttgaaaac tggtcgagat ggtattcatg gtgctacttt tgcatctgaa gaattgacgg
     5101 aagaaagcga aagtaaacga ccttctgtac aaatcggtga tccatttgta ggtaaaaaat
     5161 taatggaagc aacacttgaa gcaattacat ttgatgaatt agttggtatt caagatatgg
     5221 gtgctgctgg tttaacatct tcatcgtctg aaatggcggc aaaaggtggt agtgggttac
     5281 atttgagatt agaacaagtg ccaacacgtg agccaggtat ttctccttat gaaatgatgc
     5341 tttcagaaac tcaagaacgt atgttactag ttgttgaaaa aggtactgaa caaaaattct
     5401 tagatttatt tgataagcac gaattggata gtgctgttat aggtgaagtt acagatacaa
     5461 atcgttttgt tttaacatat gatgacgaag tttatgctga cattccagtt gaaccactag
     5521 ctgatgaagc acctgtatat attttagaag gagaagaaaa agattataat acttctaaaa
     5581 atgattatac acacatcgat gttaaagata ctttctttaa attacttaag catccgacta
     5641 tagcatctaa acactattta tatgatcaat acgaccaaca agttggtgcc aatacgataa
     5701 ttaagccagg acttcaagca tcggtagtac gtgtggaagg cacaaataag gcaattgctt
     5761 caacaattga tggtgaagcg cgttatgtat ataacaatcc atatgaaggt ggaaagatgg
     5821 tagtagctga agcttatcga aatttaattg ccgtgggtgc aacaccatta gcaatgacag
     5881 attgtttaaa ttatggttct cctgaaaaga aagaaatcta tcaacagttg atagattcaa
     5941 cgaaaggtat ggcagaagca tgcgacattc ttaagacacc agtagtttct ggtaatgtat
     6001 ctttatataa cgaaacgaaa ggtacttcta ttttcccaac accagttgtt ggaatggtag
     6061 gtttgattga aaatgtaaat tatttaaatg attttgaacc tcaagttgga gataaattat
     6121 atttaatcgg tgatactaag gacgactttg gtggtagtca acttgaaaag ttaatttatg
     6181 gcaaagttaa tcatgaattt gagtcattag atttgagttc agaagttgaa aaaggtgaat
     6241 caatcaagac cgctattcgt gaaggactat tatcacatgt tcaaacagtt ggtaaaggtg
     6301 gcttactgat taccttagct aaactaagtg cgcattacgg tttaggatta aaatcttcaa
     6361 tagatataac aaatgcacaa ttgtttagtg agacgcaagg ccgatatgtt gtttctgtta
     6421 aatcaggtaa aactttaaat attgataatg caatagaaat tggactttta acagatagtg
     6481 ataatttcaa ggtaacaaca ccatatacag agattagtga aaatgtttca gatattaaac
     6541 aaatatggga aggggcaatt gctcaatgtt taactactca ggattaaacg aagaatgtgg
     6601 cgtgtttggt atttggaatc atcctgaagc agcgcaacta acatatatgg gacttcatag
     6661 tttgcaacat cgtggtcaag aaggtgcagg tatagttgtt tctgatcaaa atgaattaaa
     6721 aggcgagcga ggattaggct tactaactga agcgattaaa gatgatcaaa tggaacgatt
     6781 aaaaggatat caacatgcaa ttggtcacgt ccgttatgct acttcaggta ataaaggtat
     6841 tgaaaatatt caaccgtttc tgtatcactt ttatgatatg agtgtaggta tttgtcataa
     6901 tggtaacctc attaatgcta aatcattgcg tcagaattta gaaaaacaag gtgctatctt
     6961 ccattcgtct tctgatactg aagtcattat gcatttgata cgtcgaagta aagctcctac
     7021 ttttgaggaa gcgttgaaag aaagtttgcg aaaagttaaa ggcggtttta catttgcgat
     7081 tttaactaaa gatgcgttat atggcgcagt agatccaaat gctatcagac cacttgttgt
     7141 aggtaaaatg aaagatggga catacatcct tgcaagtgaa acatgtgcaa tagatgtgtt
     7201 aggtgcagaa tttgttcaag atattcatgc aggtgaatat gtcgtgatta acgataaagg
     7261 tattacagtt aaatcttata cacatcatac gacaactgca atttctgcga tggaatatat
     7321 ttattttgct agaccagact caacaatagc tggtaaaaat gtccatgcag tacgtaaagc
     7381 ttctggtaaa aaattagccc aagaaagccc tgtaaatgct gatatggtca tcggtgtacc
     7441 caattcatcg ctatcagctg cgagtggtta tgctgaagaa ataggtttgc catatgaaat
     7501 gggactagtt aaaaatcaat atgttgcaag aacatttatt caaccaactc aagaattacg
     7561 tgagcaaggt gtgagagtga agttatctgc ggtaaaagat atagtagatg ggaaaaatat
     7621 cattcttgtt gatgattcca ttgttcgcgg tacgacaatt cgacgcattg tgaaaatgtt
     7681 aaaagattct ggtgcaaata aagtgcatgt gcgtatagca tcaccggaat ttatgtttcc
     7741 aagtttttac ggaatcgatg tttcaactac ggcagaatta atttctgcaa gcaaatcacc
     7801 tgaagaaatt aaagattata ttggcgctga ttcattagca tatctatctg tagatgggtt
     7861 aattgaatca attggtttag attatgacgc gccatatagt ggcttatgtg tagaaagttt
     7921 cactggagat tatcctgcag ggttatatga ttatgaagca aattataaag cgcatttaag
     7981 tcatcgacaa aagcaatata tttctaaaaa caaacacttt tttgatagcg agggaaattt
     8041 aaatgtctaa agcatatgaa caatctggtg taaatattca tgctggttat gaagctgtag
     8101 aaagaatgtc tagtcatgtt aaacgtacga tgcgtaaaga agttatcggt ggtttaggtg
     8161 gattcggtgc tacatttgat ttatcacaat taaatatgac agcgccagtt ttagtttctg
     8221 gaacagacgg agtaggtacg aaattaaaac tagctatcga ctatggtaaa catgattcga
     8281 taggtatcga tgcagtcgca atgtgtgtta atgatatttt aacgacaggt gcagaaccat
     8341 tatatttttt agattacatt gctacaaaca aagttgttcc tgaagttatt gaacaaattg
     8401 ttaaaggtat tagtgatgca tgtgttgaaa cgaatactgc acttatcggt ggagagactg
     8461 ctgaaatggg tgaaatgtat cacgaaggcg aatatgatgt agctggattt gctgttggag
     8521 cagttgaaaa ggatgactat gtagatggtt cagaagtgaa agagggacaa gttgttatag
     8581 ggcttgcgtc aagtggcatt cattcaaatg gatacagttt agtgcgcaaa ttaattaatg
     8641 aatcaggcat tgatttggca tcaaacttcg ataatcgtcc atttatcgat gtctttttag
     8701 aaccaactaa attatatgtc aaacctgtac ttgctttgaa aaaagaagtt tctattaagg
     8761 caatgaatca tattactggt ggaggttttt atgaaaatat tccacgtgca ttgccagccg
     8821 gatatgctgc tagaattgat actacatcat ttccaacacc aaaaatattt gattggttac
     8881 aacaacaagg caatatagac acaaatgaaa tgtataacat ttttaatatg ggtattggct
     8941 atacggttat cgttgatgaa aaagatgtat cacgcgcttt gaagatttta gcagaacaaa
     9001 atgtggaagc ctatcaaatt ggtcatattg tgaaaaatga gtcaactgca attgaattgt
     9061 tgggggtata acaatggtta aaattgcgat ttttgcatca ggttcaggaa gtaactttga
     9121 aaatatagtt gagcatgttg aatcaggaaa acttgaaaat attgaagtta cggcgctata
     9181 tacggatcat caaaatgcgt tttgtataga tagagcaaaa aagcacgata ttcctgttta
     9241 tattaatgaa ccaaaacaat ttgattcaaa agcagcgtat gaacaacatt tagtaacatt
     9301 attaaatgaa gataaggtag agtggattat tctagctggc tacatgcgtc taattggtcc
     9361 agacttatta gcttcatttg aaggtaaaat attgaatata catccatctc tattgccgaa
     9421 atataagggg attgacgcaa taggccaagc atatcatagt ggcgatacta ttactggttc
     9481 gacagtacat tatgttgata gtggtatgga tacgggagaa attattgaac agagaaaatg
     9541 tgatattaga ccggacgatt caaaagaaca attagaagag aaagtaaaaa aattggaata
     9601 tgagttatat ccaagtgtta ttgctaaaat tgtaaaataa ggagtatgta tgtgatgaag
     9661 aaagctattt tgagcgtatc aaataaaaca ggtattgtag agtttgctaa agcgttaacg
     9721 caattaaatt atgaattata ttcaacaggt ggtactaaac gtatattaga tgaagcaaat
     9781 gtaccagttc gttctgtttc agacttaaca cattttccag aaataatgga tggccgtgtt
     9841 aaaacattac atccggctgt tcatggtggc attttagctg atcgaaataa accgcagcat
     9901 ttaaatgaat tatcagaaca acatatagat ttaattgata tggtagtagt taatttatat
     9961 ccattccaac aaactgttgc aaaccctgat gtgacgatgg acgaagcaat tgaaaatatt
    10021 gatattggtg gtccaacaat gttacgtgct gcagctaaaa actataaaca tgtaacaaca
    10081 attgtacatc cggcagatta tcaagaagta ttgacgcgat taagaaacga ttcgttagat
    10141 gagtcatata gacaatcatt aatgattaaa gtttttgagc atactgcaga atatgatgaa
    10201 gcgattgtac gtttctttaa aggggataaa gaaactttaa gatatggaga aaatccacaa
    10261 caatcagcgt attttgtgag aacttcgaat gctaagcaca cgattgcagg cgctaaacaa
    10321 ttacatggga aacaattaag ctataacaat attaaagatg cagatgctac actagcttta
    10381 gttaaaaagt ttgatacacc tgctacagtt gcggttaaac acatgaatcc atgtggtgtt
    10441 ggtatcggtg acacgataga acaagcattt caacatgcgt atgaagcgga tagtcaatca
    10501 atatttggtg gaattgttgc attaaaccga gctgtaacac ctgagttagc agagcaattg
    10561 catagtatct ttttggaagt cattattgca ccaaaattta cagatgaagc attagatatt
    10621 ttaaaacaaa agaagaatgt aagattatta gaaattgata tgactataga cagtaacgaa
    10681 gaagagtttg tttcagtatc tggcggatat ttagttcaag ataaagacaa ttatgtcgtg
    10741 ccaaaagaag aaatgaaagt tgttacagaa gtagcaccta cagatgaaca atgggaagca
    10801 atgttattag gatggaaagt tgtaccatca gtaaaaagta atgcaattat tttaagtaat
    10861 aataaacaaa ctgtaggtat aggtgctgga caaatgaatc gtgtcggtgc tgctaaaatt
    10921 gcgttagaga gagctattga aatcaatgat catgtagcgt tagtatctga tggatttttc
    10981 cctatgggag atacagttga acttgcagca caacatggta taaaggcaat tatccaaccg
    11041 agtggttcga ttaaagatca agattcaatt gatatggcta ataaacatgg tattgcaatg
    11101 gtagtcacag gcactcgaca ttttaaacac taattttaaa ggggcgtaac ataaatgaat
    11161 gtattagtaa ttggtgctgg tggacgagaa catgcacttg catataaact taatcaatcg
    11221 aatctagtta aacaagtgtt tgtcattcca ggtaatgagg caatgacacc tatagctgaa
    11281 gtacacactg aaatttcaga atctgatcat caagcgatac tagattttgc taaacggcaa
    11341 aatgttgatt gggtagttat aggtccagaa cagccgctaa ttgatggatt agcagacatt
    11401 ttacgagcga atggtttcaa agtgtttggt ccaaataagc aagcagctca aatcgaaggc
    11461 tcaaaattat ttgctaaaaa gataatggaa aaatataata ttccaactgc tgattataaa
    11521 gaagttgagc gaaaaaagga tgctttaaca tatattgaaa actgtgaatt gcccgttgtt
    11581 gtcaagaaag atgggttagc tgctgggaaa ggcgttatta ttgcagatac tattgaagca
    11641 gccagaagtg ctattgagat tatgtatggt gatgaagaag aaggtactgt tgtatttgaa
    11701 acgtttttag aaggtgaaga gttctcgcta atgacatttg ttaatggtga tttagcagta
    11761 cctttcgact gtattgcaca agatcataaa cgcgcatttg atcatgatga aggaccaaat
    11821 actggtggta tgggggctta ttgtccagta ccacatatta gtgacgatgt tttaaaactt
    11881 acaaatgaaa caattgcaca acccattgca aaggcaatgc ttaatgaagg ttatcaattc
    11941 ttcggtgtat tatacattgg tgctatttta actaaagatg gtccaaaagt aatagaattt
    12001 aatgcccgtt ttggtgatcc tgaagctcaa gtattattaa gtcgcatgga aagtgattta
    12061 atgcagcata ttattgattt agatgaagga aaacgtactg aattcaaatg gaaaaatgaa
    12121 tctattgtag gggtcatgtt ggcatcaaaa ggatatcctg atgcatatga aaaagggcat
    12181 aaagtaagtg gctttgattt aaatgaaaac tattttgtta gtggattaaa gaagcaaggt
    12241 gatacctttg ttacttcagg tggtagagtt atacttgcca tcggaaaagg tgacaatgta
    12301 caagatgcac agcgagacgc atacaaaaaa gtatcacaaa tacaaagtga ccatttattc
    12361 tatcgtcatg acattgcgaa taaagcacta caacttaaat aagtaaattt aaaatactaa
    12421 gattagctat gaacgaatct ataacgatag attttttcat agctttttta gttgtagagt
    12481 ctaggacatt gatttctgta ccaaatttgt gattatgcat atgtaataca aaagaggcgc
    12541 cacaacatgt ttggatgaac aaaataacat gtttgtggca cctcttttgt ttagtatgga
    12601 ataaatggtt ttctttttct atacaatgaa tttctaattt agtatctata caattatgga
    12661 taaaatttaa cctacacgac caagacgaac atcatctatg cccgtgatgg gtaaggtgat
    12721 tgaacaataa tatgccatag taataatggc aattaaaact ataataaaga ttatatcttt
    12781 atatgagaaa ggtacgttgt aatagtaagt acgaggacca tctctaaatc ctttcgactc
    12841 catcgcaact gataattgat gtgcctttct aatattttgg cttaatagag gtataattaa
    12901 atgcttaaat cgctttaacc ctctataatt tgccgcgtct atcatctgat agcgcatttt
    12961 taaagatctg cgaagctgta ttaaagaact aatcattaaa ggtatcatac gaatggcagc
    13021 catgaatgca taagcaactt ttgatttaac ctttaaatgt tgcattaaac tataaaatat
    13081 catgacaacc tgagatgtaa gtgcgattaa gataccgaaa aatgaaatag caatggttct
    13141 taatgataca tgtaaaccac gaactaaact ttctgttgta atatggataa atccgaattt
    13201 caaaattgta tggctaccat tcccgtataa aatcatgaac agggaagaga gtaatgcaaa
    13261 gccaatactt atagttataa aaattgctgt aattttaaac tgagtaccat taaacatcaa
    13321 taagaaaact aacattaaga tagtgatata aagcataaaa tcgaaattat gcacaaatat
    13381 aataaagaaa aatagtataa ttccaagaaa tagtttcgtt ataatgttga catcatcaac
    13441 aaatgattgc cgaactttcc attgctcata cattcgtatc accatcacaa tctagtaacg
    13501 caccatctga aattttaagt cttcttgatg gataacgttc aattatttca tcgtcatgtg
    13561 taaccatgac aatactttgt cccaaattaa ttcgcttttg gaaaagtttg atcaactgga
    13621 atgtattatg gctatcaagt ccaaatgtcg gttcatctaa aaagataata tcagctttag
    13681 aacttagtgc ggtagctacg ctaaggcgtc gtttttgacc aatagacaac tcataaggat
    13741 gttgatcttt tacattttgt aaatctaaaa gttttaaaag ttgtatcgtt tcatcatcac
    13801 tttgatcttt agaaaggtga ttaaaatgaa tgttaatttc atcataaacc gaatttgtta
    13861 taaattgtaa ttctgggttt tgataaacta ggtacatgtg ttttgctgca tgtttaattt
    13921 ttgttaaacg ctgattttca aaataaacat caccttgata tttaatcaat tgcataattg
    13981 attcaagcaa ggttgtttta ccactaccat ttgcccctgt aattgtaatc cactcaccta
    14041 gaccaatttc taaatctgag aatgagagca atgttgattt accgcgaata atacgtccat
    14101 ttttaaattg taataagtgt gagtttgttg ttggaaagtc aacacgactt ggtgcgaatt
    14161 cccatgcacg tggatgccac acaccatatt cactgagtaa atgaacatac ttctgtaata
    14221 tgatttcagg acattcatcg gcaatgatat ttccgttata atccatcaaa atgacgcggt
    14281 cgacatgatt ccagatgtgt ttaactttat gttcaacgat tacaaccgtt tgatcttccc
    14341 aaagttcaat tagtttagtc cataaatctt ctgttgcttg aacatctaac attgctgtcg
    14401 gttcatctaa aaacaatgtt tttgattgtt gaagaatggt ttcaacaatt gccaatttct
    14461 gtttcatccc gccacttaaa tctttgatat acgtttcagg ggtaacattt aaattgacca
    14521 tatttaaagc attgataatt aacgcatcca tgtcttcacg tggtaattgt ctattttcta
    14581 aaacgaatgc aagttcttcg tatacttttg gcatacaaaa ctggctatca gggtcttgga
    14641 aaataacgcc acttaatggg tcaacgatta gttcatcata tttcataggt aattcaatta
    14701 aattaggaac aataccactt aatacattca gaagtgtact tttaccgcaa ccagaaggac
    14761 cgagtaaaag tactttttct ttgtcttgaa tagtgatatt taaatgatcg aaaattttac
    14821 gttgaccact tggatatttt aatcgtaaat cacttacttt taacactctg aatgctcctc
    14881 ttataagttg tcgtaatctt ctttagcagc tggtctaaat aattttgtta cgcctgtctt
    14941 atctaaagct tttactaaaa ggtaagatag gacgccggcg actactgcac cactaattaa
    15001 tctaaatacg atgaataatg ttaagttcca acctgcaact tcatttaaat aaccatagaa
    15061 ataatctatc gggaaagccg cgattgctgt acaaaaacct gctaacatag ctaccataac
    15121 tgaacgtgat tgatatttaa aaattgcaaa gacaagttca cacgctaaac cttgtataaa
    15181 agcgtaaacg attgtcggaa tatcgaaacg acccataatg atagtttcgc cggcacctgc
    15241 agcaaattca gccagtaaag caatacctgg ttttggaata attagatagc agacaatcgc
    15301 tgccatgaac caaaccccgt ttgttaattg ttcgaggtga aggcctgtag cttgcacacc
    15361 attgtaaaca aaccaccata aattgtaaat aactgcgaat actactgaaa taagtacggt
    15421 tactagtatt tcagatagct ttaaaccttt tgacattttt acatcctcct aataaaaaaa
    15481 cgcacaacca tccataggaa agttatgcgt tcacaatata tattagtaaa acatatgtat
    15541 agtaacactt tcctacgcta gttcaagcta gatcaggttc aaagggtttg agggcaagcc
    15601 tcatctcagt ataaaacacc cc
//
LOCUS       DAKTIP010000046        15596 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAKTIP010000046 DAKTIP010000000
VERSION     DAKTIP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15596
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.046"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(444..3500)
                     /gene="fnbA"
                     /locus_tag="O0305_001102"
     CDS             complement(444..3500)
                     /gene="fnbA"
                     /locus_tag="O0305_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCY0274252.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAP
                     QTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVE
                     PHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGE
                     VLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELD
                     VKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNG
                     NQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLD
                     KTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGN
                     GKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGY
                     VDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPVEEITENNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKD
                     KPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLP
                     KVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(3484..3585)
                     /locus_tag="O0305_001103"
     CDS             complement(3484..3585)
                     /locus_tag="O0305_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274253.1"
                     /translation="MLLKTEQYRLAFIKFKKLMNFAFKGRYYSEKQS"
     gene            complement(3969..5327)
                     /locus_tag="O0305_001104"
     CDS             complement(3969..5327)
                     /locus_tag="O0305_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCY0274254.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(5444..6997)
                     /gene="gntK"
                     /locus_tag="O0305_001105"
     CDS             complement(5444..6997)
                     /gene="gntK"
                     /locus_tag="O0305_001105"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCY0274255.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVEALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNKDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIEENVTVYQEIVSIFINLSRSLTENYEQIADFQR
                     QHIAENKTQ"
     gene            complement(7021..7701)
                     /locus_tag="O0305_001106"
     CDS             complement(7021..7701)
                     /locus_tag="O0305_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0274256.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(7855..8619)
                     /locus_tag="O0305_001107"
     CDS             complement(7855..8619)
                     /locus_tag="O0305_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0274257.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(8784..9476)
                     /locus_tag="O0305_001108"
     CDS             complement(8784..9476)
                     /locus_tag="O0305_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0274258.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(9707..10123)
                     /locus_tag="O0305_001109"
     CDS             complement(9707..10123)
                     /locus_tag="O0305_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCY0274259.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(10262..12085)
                     /locus_tag="O0305_001110"
     CDS             complement(10262..12085)
                     /locus_tag="O0305_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCY0274260.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(12563..13840)
                     /locus_tag="O0305_001111"
     CDS             complement(12563..13840)
                     /locus_tag="O0305_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274261.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            14387..14998
                     /locus_tag="O0305_001112"
     CDS             14387..14998
                     /locus_tag="O0305_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCY0274262.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVVIIIAVIYFAIRYIGKRKRVK"
     gene            complement(15007..15105)
                     /locus_tag="O0305_001113"
     CDS             complement(15007..15105)
                     /locus_tag="O0305_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274263.1"
                     /translation="MPNKLKKAVNHFNRWNDSLLFVYRNLKFMPLC"
     gene            complement(15095..>15596)
                     /locus_tag="O0305_001114"
     CDS             complement(15095..>15596)
                     /locus_tag="O0305_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274264.1"
                     /translation="SILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRFSK
                     KYNEIMILRVTITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKR
                     FEHIKGKVLGVFNFVRYIGMTVGALLIGIISQPLVAFYFTTITIMLIVIFLYIKIVDF
                     QLKYAK"
BASE COUNT         4741 a         2999 c         2261 g         5595 t
ORIGIN      
        1 aaattcaaat tcatctaatt taaaacaata tactaaacca tacataataa tcgcctgtac
       61 aatgcatcat taacaagtca ctgaaacgcc tttcattgta ttaataacgt cactataatt
      121 tttatatcgt tcggtttttg tttgatttta atgattattt atacaaaaac agccgtattt
      181 caagccgaca ttttaaattt aactaaattt gcatctagtt aataattgca tttatcaaat
      241 ttgtcttatt gatccaatct aatttgtact cacaaactag tttaaaattc taactttatc
      301 tctcagttcg ttatcaatca tcagacataa accaatgaag caatcagaaa acactctaat
      361 tttctattag aaatttgatt taatataaaa aaacaggctt acttcatata atttatgaaa
      421 taaacccgtc aatttttgtt taattatgct ttgtgattct ttttatttct gcgtaataat
      481 gctaaaccta gaatgctgaa taatccgccg aacaacatac ctttgtttgt tgattcttct
      541 ccacctgttt caggtagttc agatttctta gattgtggtt ttttagttgg tgccactgct
      601 ttaacctttt cattgatttc aataacaggt gttactactt taccttgttc cactggttta
      661 gaaggctttt taggttcttc tttggcaggt ggtactggtt taccaggttc agctggtacc
      721 tctggtgttg gcggtgttgg agtttctggc tcactcggca cttctggtgt cggtggtgtt
      781 ggtgtttccg gctcacttgg tacttctggt gttggtggcg ttggtgtttc cggctcactt
      841 ggtacttctg gtgtcggtgg cgttggtggc acgattggag gtgttgtatc ttcttcaatc
      901 gtttgttgac cttcattttg gccgcttact tttggaagtg tatcttcttc aaagtcaaca
      961 ctattgtgtc caccgaattg ataacttggt ttatctttat ttgtatcttc ttcaataatt
     1021 tcagtgtgct tattgaatcc gtgaatatgt ggcacactgt cgaagtcgat atcaatgatg
     1081 ttaccgccat gttcatactt aggtttgtct ttttctgtat cttcctcgaa tgactgatta
     1141 cctttatttt gaccatgaat ttgaggtaca ctatcaaaat cgatatctac gatattgcca
     1201 ccttgttcat atttaggttt gtcttcttct gtgtcttcct cgaatgactg gttaccgcta
     1261 ttttggccac cttcataacc taattcactc ttaatatcaa cgtggctatt ttcttcgatt
     1321 tcttcaatca cgtcataatt cccgtgacca ttttcagttc ctaaaccaga atgagaaata
     1381 tgatgattgt tttcagtaat ttcctcgact ggtccttgtg cttgaccatg ctcttcaggt
     1441 aattcatcca ctaattcaat cagattactt tcagttgtat attctttcgt atcttcaact
     1501 gttgtatgat cgctcactgc gccagttaca ataccttttg tagactcttc gtcaaattca
     1561 actaagttag actcagtagt aacctgacca ccacctgggt ttgtatcttc ttcatattca
     1621 acaacatcag cgtgatgttt tgaattttca tgtgtagatt cttcaaagtc aattggattt
     1681 gattcctcag aggactcagt gtatcctcca acgtgacctg cttcgctatc cacagcagta
     1741 tggtaatcga tatcaatagc tgatgaatcc gtttcttcta ttgtttcaat gtatccatca
     1801 acatatccac ctccaccatc tatagctgtg tggtaatcaa tgtcaagagt tgatgaatca
     1861 tattcctctt caacagtagt tactaaattc ttatcatatt gacctgtaag agtttcttta
     1921 attgtatctt ctttatattc aaatttatta ttttgaataa tcggaccatt tttcccattt
     1981 ccgttcgctt tattactgta taaaactaaa ccattatccc aagttaaggt atatcctcta
     2041 tcataataat acttataaag ttgctctgga tgtcctacca tttgtgttct aaaatcaact
     2101 tcatcagtac catttaaata ctctccatca tagtgaacaa cataagtttt atctagattt
     2161 tctatattca atgaatagct tccattattt tgtaaattca aattcccact catattactt
     2221 gtgacttctt taaatttaga agtatctgtc gtatttgcat atacactctt cgctatgtct
     2281 tcattattac ccaagtattc aaatatccta acttttggtt gatttccatt ctgattacta
     2341 cctttcatta aagttccagt aacagtcaca cttgtcgttt taccattatt aggtttaata
     2401 aatgcaacat gcgaaaatct attattcgct ttattaaatg tctcaatcga tccatttaaa
     2461 ttggcataat aattcccaat accatcttta tatttaacat ctaattcctt tgaagtttgt
     2521 tcttcattta gtgttgaagt tatagtttga tttccattag tttgtacagt tttaggatca
     2581 ataaataaat taatttctag ttcagccgtt acatcaacct tatcttcaat atcatttgta
     2641 aatgtatatc taatctttcc accttctaaa acttcacctg tcgccattac gactgaacca
     2701 tttttaattt ctggtacttt tctagcagtt gatacgccat gcgtatttac attatttgat
     2761 aaagtaaagt caaagtagtc accttgatgt aaaccattct caaatttcaa cttatatttt
     2821 agtaccgctc gttgtcctgc atgaggttct actttatttg tattgttatg cccctcaata
     2881 gaaccaattt ctactgtaac tttacttgtt acatctgtac ccgtttccac tttcgcgtta
     2941 ctagcttcct tagcttccgc tacatctgct gatcttgtca cacgtggctt actttctgat
     3001 gccgttcttg gctgtgccac ttcaacttgt gtttctgcga cttgattttg tgtagccttt
     3061 ttaggtgtta aatctacttg tctttgatct ccgctattgt cttgagattg tgttgtttcc
     3121 ttaacttgag gtttcgcttc ttccttaact acctcttctt taactgtttc tatatttgct
     3181 ggttgtgcag tttgtggtgc ttgtactgct tttggtgctt cttcagttgt tacttgtgtt
     3241 gcgtttgacg gttgttctgt tactgttgcg ttatatgatt gagtttcttc tatatgatta
     3301 acgttagttg cagttgtttg tgtttcactt gttttattat cagtagctga attcccattt
     3361 tcttctactg tagttgtctt ttgttctgat gctgcagctt ctttgtcttg tcccatccca
     3421 acaacgatca ttgttcctaa gaatactgat gctgctccca atttatgttt tctaatgccg
     3481 tacctaagat tgtttttcac tataatatct ccctttaaat gcaaaattca ttaatttttt
     3541 aaacttaata aatgcaagtc tatattgttc ggtttttaaa agcaatacaa aaaagcgagt
     3601 taatgaaaag ttaagattgt cattaatttt atgttaattg ataattttat tatttgaaat
     3661 atacctataa attgtattca agtcatcaga aacccttgtc acacaaggct tgtatttttt
     3721 atacttattt ttttaaatta aattcatcat tatctaattt aaaacaatat actaaacgtt
     3781 tcataattat cgcctgtaca atacgcacaa aaacatgtct tgaaacgcct ttcatcactc
     3841 taaaataccc aatatacttt ttatatcgtt cggattctga gtatttcaga cgattttctg
     3901 cataaaaata aacgtgtttc aaggcaatat attgcaatta cctaaaaaca cgtttactta
     3961 atatttagtt aaacaaataa gctaatgaat aaaatgaaga tgatacctga aacggaaata
     4021 atcgtttcta ataatgacca tgttaagaat gtttctttta cagttaaacc aaaatattct
     4081 ttaaacatcc aaaatcctgc gtcatttaca tgagacaaaa tcacactacc tgcacctatc
     4141 gcaagtacaa ctaatgcaac atttacatct gatgattgta ataatggtaa gacaatacct
     4201 gtagttgaaa tcgcagctac tgtagccgaa cctaatgcga tacgtagcac agctgcaaca
     4261 atccatgcta gtaaaatcgg agacatctct gtaccttcaa acattttagc aattgtattt
     4321 ccgacaccgc cgtcaattaa tacttgttta aatgtaccgc caccgccaat aatcaataac
     4381 atcattccga ttggataaat cgcattcgtc actgattcca taatatgatt catcttacgc
     4441 tttctcatta atcccatcgt aacgattgca aataatactg ctattagcat ggctgtccct
     4501 gctgttccta tcatataaat gatagattca aatagatttg taggtttgtc atgcccagtt
     4561 acaagttgcg ttatcgtaga cactaacatt aatatgactg gtaatgttgc tgttaataaa
     4621 ctcataccaa atcctggcat ctcttgatcc gtaaattctt tttgtgcacc taacgctgaa
     4681 atatcgcctt ctcgtgtata cgcagacgga atcatttttt gtgcaacttt gttaaatata
     4741 ggccctgcaa tgagtgtaac tggaatggca ataatcatac catacagtaa tacatctcca
     4801 acatttgcct ttaattcttt tgcgatgact accggtcctg gatgtggtgg taaaaagcca
     4861 tgtgtcactg ataaagctgt taccataggt agtcctagtt ttaacactga aacatttgcg
     4921 cgttttgcta ctgtaaatac taatggaatc agtaagacta aacctacttc aaagaacaat
     4981 gcaataccga cgataaatgc tgcaacaagc attgcccatt gtacatgttt ttgaccaaat
     5041 ttttgaatca acgtgtctgc gattcgagtt gcaccaccac catcagcaag caatttccca
     5101 agtatggcac ctaaaccgaa tatcagtgca atgtggccga gcgtactgcc cattcctttc
     5161 tcaatcgtct ccataatttt agtcaatggt atacctagca ttaacgctgt aatcatcgat
     5221 gtgataatta atgaaataaa tgtatttaat ttaaacccaa taattaatac taataaaata
     5281 acgataccta aaacaacact gattaacggc catatttcgt taaacatgac attcccctct
     5341 ttctcttttc aatagaatgt aacaccgtcg tcgcgcacgt attccacata atatatagta
     5401 gtcatggcac aacaacttta tctaagatgc ttgagtgacg tatttattgt gttttatttt
     5461 cagcgatatg ttggcgttga aaatctgcaa tttgttcata attctctgtt aaagaacgac
     5521 ttaaattgat aaaaatggat acgatctctt ggtaaacagt gacattttct tcaatcggcg
     5581 tatgattgtt tgtggcaccg accatcgatg aaacgattga aaaatcttca atgtcaccta
     5641 cagctttaag tccgagcacg caggcaccta agcatgaact ttcataactt tcaggaacca
     5701 ctaactctgt gtcaaatata tctgacatca tttgacgcca tacttcactt ttcgcaaaac
     5761 cacctgttgc ttttatcatc ttaggtgttt cattcattac ttcaataagc gcaagataga
     5821 cggtatacaa attgtaaaga acaccttcta atgcagcgcg aatcatatgt tcttttttat
     5881 gagataaagt taaaccgaag aatgaacctc ttgcatttgc gttccaaagc ggcgcacgtt
     5941 ctcctgctaa atagggatgg aatattaaac catctgcacc tggtttaaca cgctttgcaa
     6001 tttgagttaa gacatcataa ggatcaacac cgagacgttt cgcagtttcg acttcactcg
     6061 ctagcaactc gtcgcgcaac catctcaata cgacaccacc attatttaca ggacctccga
     6121 tgacgtagtg gtcctctgtt aagacataac aaaatattct acctttgtaa tcagtacgcg
     6181 gtttatctat cacagtacga atcgccccag atgtaccgat tgtgacagca acttctcctt
     6241 taccaacact attgacacct aaattagaaa ggaccccatc actcgcacca ataacaaacg
     6301 gtgtatcttt attaagcccc attaatgttg cataacgttc tttcatacct ttcatcacat
     6361 acgttgttgg aactaattcc ggcaacattt ccttggaaat acccagcagt tctaatgcct
     6421 caacatccca atctaatgtt tctaaattaa acatccctgt tgcggaagcc attgaataat
     6481 caatgatata tgtatcaaat aaatgataga aaatgtatgt tttaatatct gcaaacttag
     6541 cagtacgttg aaatacatct tgccattcat gtttcatcca aaaaatcttc gctaatggcg
     6601 acataggatg aatcggtgtg cctgttcgct ggtaaatcgc attgccatca tgcacttcat
     6661 ttattactgt tgcatatttt gcagcgcggt tatctgccca agtaatatta tttgttaatc
     6721 tttgatgttg ctgatccatc gcaatcaagc tatgcatttg cgcactaaat gacacaaact
     6781 taatgtcgtc tttattaact ttggattctc tcataacata tttaatagtc attagtactg
     6841 catcaaataa ttcatctggg ttttcttctg agacatcaac gtttggtgtg tgtaaatcat
     6901 agcctatttg atgtttcatg ataaaagttc cattttcatc atataagact gacttggtac
     6961 tcgtcgttcc aatgtcgaca ccaatcatat atttcatgat aaatccttct ttctttcatt
     7021 ttaattcaac caaaatcctt caatatcttt accaacatcg tcgaaattta aatgaaacgc
     7081 ttctttcaaa atttgactgt cgtattgttc cactgcatca ataaacactt gatgattatg
     7141 atgtatgcgt tcaaaatctt gcgggttctg ttgcatacgt tgtcgcatgg atgttaaaac
     7201 gagtgccatc attactggtt ttaaatgact ccaaaatgag tttaaatatt gatgattaga
     7261 tgctttgatt aatgtttcat gaaattcaaa gtcatgcttc gtaaatgatt ctgcatcctc
     7321 aaattttact gccactttca tcatttcaag ttgtttcttc atttctttta cgatgggtag
     7381 tcgctcttga tttttaactc ttgaaaatgc aaatgactct aacatcagtc gcaaatcata
     7441 catttctttc ttttcttgtt ccccaaacgg caacacatgt gcacccattc tttctaattg
     7501 gatgagttga ttttgttgca ataatttaaa tgcatctcga attggcgaac gactcacatt
     7561 aaattgcttt gccatttgat tttcagtgag taacgtacct tcagctatgt gaccattcac
     7621 aatgcctaag cgtaattctg ccgcgatacc ttctccagtt gtcatacctt ccaaccattt
     7681 ctctggatat ccatacatca tcaaagtcac tccttcatta cacgacatac ttgtatacaa
     7741 gtatgttaat atagttatta tgagtttgca agcgctttct ttacgagcac taaaatagtg
     7801 accacccctt ttcgatttaa atttaaagga aatggtcact atcacacgaa tgatttaatt
     7861 gttatgttgt atgtgggata tttctaattg ttctgtactc atatgcgctt taggtacttc
     7921 aatgcaataa tgcgtttcat gacagtttgg acattcgaat cgacgtgttg tcgctgtatg
     7981 tttcgctttg ataactgccc acaaagatgg tgagaatata tgctggcagt taggacataa
     8041 ataggcaacc ttttgttggt aataaaaagt aacaccaatg ccataaccaa tcataaatgg
     8101 taaagcaatt aaaaacggcc atttattttt catcaaaatt gcacttataa tgctagaata
     8161 ttgaattatt cctataatac cagcactaat ccaaatgtta cgacgaatac ttttcatttc
     8221 agctgattta ctcatgacat gctctatgtc ttttaagtgt gtgattggag acgtcgacgc
     8281 ttcatttacg taatattgaa catttttaat tttgtttaat accgcttgtt gctgtttaac
     8341 ttgttggtta atttcttgtt gtttcatagt tagtaaagta ttgagcgtct tcaaagtacc
     8401 ttcacctttt agcaacatat ctatatcgct taacgcacaa cctaaatctt taagcaataa
     8461 gattaactct aatgtttgtc gctgttgttc tgtatacaca cgacgctttc cttctgtaaa
     8521 tccttgtggt ttcaaaatac ctttgcgatc ataatattga atcgttcgtg ttgtcacatt
     8581 gcataatttt gcgagttctc cagtcgaata gttagacata gattccacct cctataatta
     8641 ccatagttga tgacccgacg tcacgagcaa gtacaatttc cacattttaa agaaatttat
     8701 tatactaggc gtcttatttt tatgatttcg taccatgttg atttacaaac tcactcaaac
     8761 taagtaacac acctactaaa catctactct gttatttcag aatgaatttg ttgtaattta
     8821 tcttcaactt cagtaatctc tgtcgcacat tctttcagta aatctcgata cttttccgtc
     8881 tctgcattgt ttttataacg tattttatgt tctaaacttg cccacatatc catacctatc
     8941 gttctaattt gaatttcaac aggcaatacc tcaacgcgtt ctgctaaaaa gactggaata
     9001 gatacaacga tatgtaaact gcggtaacca ttttctttag ggtgctgaat ataatcttta
     9061 cgttttatca attgtacgtc ttcttgttta agcaacatct cttcaatcaa ataaatatca
     9121 tctaagtaat tacatactac gcgaattccg gcaatatcca gtatatgttc tttggcagtt
     9181 tctgcgctaa tctgtaatcc tttacgatta agcttttcta ttaaactacg catttctttc
     9241 acacgtcgct ccatatgatg tataggattg tgtttataaa tgtgattgaa attgtcatct
     9301 aaaatactca gtttagtgct tatttccttt agtgctgacg aataaatatg atctaactct
     9361 acgaaaccta ataacgtatc aaatgcttca tcaccatttt caaatttact taagctattt
     9421 ttaaaatcat gtcgcaaatc ctctaaatat agtgatggtt ttcgatctac atacattttt
     9481 atactaacct ccgatatata atcatcttta ttgtacctaa cattttatta agataatact
     9541 aatacactat cgaactttgg gctgatactg aaacagcaca aagaaactca aacaattcga
     9601 attatgtatc aaaaccttca attacaggaa gtgattttaa aatgatttaa cgcaaaaaaa
     9661 cacctgttac cgttatatag gtacaagtgc ttaattgata gagatgttat acgtctttaa
     9721 atgattccac aacttttgga tgtggaccat ccataagcgg ttctctttgt cgaacgccac
     9781 cactttggtt gccaattgat tcgctatcaa agtacgattt atcttgattt gattgttctt
     9841 gaatatgttc ttcattatca gttgttgcat attcactatc cacctctgtt ttttccattg
     9901 ttgctgtatg gaattgtacg aagttctctt cttctaatgc tttgatttct tctttcgata
     9961 acgctcgata ccaatctttc gcttttttcg ccgcaatagg aacaacatct ttttcaagat
    10021 atccttcttt taacattgca ttcgcaattt ctatcgcttt ttgccgttta attacttcaa
    10081 attctttcca tttttcgggg taacctttca ttgtaaaggg catttcctta acctccaata
    10141 tgttataatt catgttatat accaccataa ttataaaatg aaacatatta aacacatttt
    10201 tttagactcc cgtcataata acttcagaca aacgttagtc tgacctgata aatatttaat
    10261 tttaaacgtt cttgtctctc tcacgaaaac cattgtgttg aatattcatc attgctgatt
    10321 gaatataatt atactgtggt aaatctggta atatctttat ttctaattct gtatttaatt
    10381 cgaaatgctt agcgatgttt tcaaatattg ctaaatactc gcccattaat tgttcattta
    10441 ttgtaagtct atcttcatta gccatggctc tatttaacat aaagctaatt tcttctaatg
    10501 cgaataagct aggataataa ttttgaatca atgtcttatc actaaataat tcaccattag
    10561 ctgcattata aacttgcgtc atgttattta atttcacaga taggttcata gccgtgtttt
    10621 tctgatacac atcatcttta tattgatttt cagaaaataa ataatgaaag agcgttgctt
    10681 ctttacgaac aacttcagcc attacattag gaagcaatgc ggatgcggta cgttgtccaa
    10741 ggacaaataa acctatgatt gcaatcacaa tacccattgc aacgtcaata actcttggaa
    10801 acgcaatgtt aattgttaaa ttctgtgatg ctaatccgtt cattaaaata acttgtattg
    10861 taataaaaac tactgcaatc gcataatttg ctcccaccaa tgcttcagta aacaatgctg
    10921 caatgcccat taaaattact gcaacaggtg ttggtattga aaacaacaat atgactgaaa
    10981 gtactaacac acctaaaata gtacctaaac ctcgtgccat acctctctcg attgcatgta
    11041 tagttgatgt tcctagtaat attgtatgtg cagataacgg tatccagtat gctttttcaa
    11101 aatcaaacat taacgcaata aatatcgcta tcatcataat cgctgtatat ctcaatgtat
    11161 ttctaaaaac aatagagtct aatgttaaat tttgataaat gcgtttacta tataaaggtt
    11221 ttcgcatgtc tgcttcatac gcaatacgcg cttcatttgc atgcaccatt tcatcaattt
    11281 tcaatatatg gttgaataaa ttttgaaatt cctctgttac tgtcacttct tttcgccaat
    11341 atcttacatt ttcctcacta ttatctagtt gtgcaatgat atgatccatc atttcaatta
    11401 actcatctgg caatggtcga atttgtttag cgttcaactc taacagttca gaataaatcc
    11461 cttgggcaga tgtgtgtaat aataataatt tttggaaacg tctacttaat ttatcgttac
    11521 ttgaagtaga agtgattaat aatttatcag atgctttaaa actatccacc gctgtttttg
    11581 ctacttgtaa aaaagcagat ttatcattat aagtgtgtaa caacttagat atgagtttaa
    11641 aatcattttg aattgcttgt tcttcagctt tgtttttaga aaatacgatc gtgattaaca
    11701 ccatcatagt ggcaagtata ccgcctacta aaatcgcaaa gcctctatat aatgcttctt
    11761 cgggagctat aggtaagtta atgggtagac tgaatgtcac aatgaagaat gtcgatgacg
    11821 gtccagctat ttttaaggca gtaaatatat aaaatggtat aaccgtaaca attaataatg
    11881 tcattccaaa aacgagtggc gttttggctg ttagcgtacc aagcatcata catattgcaa
    11941 acgctaaatt acaaattatt acagttcgca gcttagatcg cgacggacct ttaaaaacat
    12001 aaatatgggc tagtgttccg gttgcaacta ataatccaaa ttggaaatta ccacataagt
    12061 aaccgattat tgcaggaata atcattaaca gtccctgtct tacaccttta taaaagtcta
    12121 agttaagtcg attgattttt atgagtgatg tcaaaaagat gtcatttcga cttctccttt
    12181 ccttgtcaaa atatttccat aatcttttta ttagcatagt taaaatgaat ttgttgctat
    12241 aaattttaat tacgcaacat atgtcattat aacaccttat gctcaattat ctaaatttaa
    12301 aaacttttaa agtccaatta aaattaattt ctaatgttat ttaacatcgt tgaacctttg
    12361 gtcgcccacc tttgcgctag ttaaaattta tatgtacttt tacaagcgtt acctgactaa
    12421 tgttttaaaa ccttatttct ttacatatat catttcaata acttcatttt catcttaaac
    12481 catatctgtt ttaaaaagtt tcaatttcaa cgcaaaaaaa gcaataagcc ccaactatgt
    12541 gaaagcttac tgcttgttta aattattttt ctattgcttt atcatcacta acaattaaag
    12601 gttgatctgg tttcacatag aaccacataa tcacagcgat tactgctgga acgattaaca
    12661 attggaatgt cattgtccaa cccaaatgtt ctacgaatag acctgctaat agcggactga
    12721 ttaatgcacc tatattaccc cataagttca tccaccctga tactgtacca gagaagttac
    12781 gtcctaagtc agttgctgca gcccaactca tacccattga aataccgata ccaccaagac
    12841 ctagtgataa ccagaaaata cttacatata aatttcctgt gcgtacagca aagataattg
    12901 aaactgcaaa cacaataaat cctgcaattg caattacacc tctagctacg aattttgaac
    12961 gacctaatcc taatacacgg tcagaaattg cacctgctga taagattaag aagaacatta
    13021 ataaccaagg taatgaacta atgctcattt ctttaaagtt aacgtggaat acttctgtta
    13081 aatacgtcgg taaccaaatt aagaataatg taatgataaa ttgtacaaca aagtattgaa
    13141 ttgcaattgc atagaagcta aaacgtttaa agaaatcatt ccatggtggt gatgactttt
    13201 cagtagctac gatatcacga ttttccataa tgaaacgttt ctccgcttca ttaaccattc
    13261 tatgttgctc aggtaagtct ttggcaataa tcgcccataa tacagccatt aaaataccta
    13321 ctgcaccaaa aatgtaaaat actgcttgcc agttaaatgc gttaacaata gcaattgtaa
    13381 ctattggtgc taatacaggt ccgaaatatg atcctgctaa taatgcactt gatgctctac
    13441 ctttttcatt tttagagaac caaaatgaat taaacacagc attagaaggg tacattggcg
    13501 cctcacctac accaaataag aatctcacta aataaattaa accgtggttc ttaatcatac
    13561 ccgttaagat tgtaaatgca ctccaccaaa ctaatgcaat tgtaatcatc ttacgtggac
    13621 cgaatttttc agccaacatc cccgatggta cttgcattaa tgcataacct agagagaaga
    13681 atgatgccaa taaaccaaat tgtggttttg tcatccctaa atcatccatc atttgcttag
    13741 cgatatatga aatgtttgct ctatccatat acgcaataac cccaataaag aagaacgcaa
    13801 gcgcgaacat ccaccttacg ttagttcgtt tttccttcat aactattcct acttccctaa
    13861 ttaatttttt aaaacttacc ctgaaacgtt aaaattttcg gacatctggc atgtccaccc
    13921 ctactttgtc acaaaattgt accaaagtta ttttttataa tattatcaga catggaaagc
    13981 gtttacaata gaatctataa aaattttatt aaattccgct taacttttgc tattaaaacg
    14041 atataaaggt acttttagtt acatagtagc ctatcgtttg ttaattaaaa atactgcaat
    14101 cctagtgttt taaaacgtat attgcaggct ttcttacata ttcgacattc attttaaatt
    14161 gcatataact acattaacca ttcattgtgc agcgcctgtt gtttagatga tcatttattg
    14221 actttttgct cttatagcaa gcaatttttt gatttattgc ttataataca ttttaagaca
    14281 aatgtttgtt tcaacatcgc atgcgacatc attaattatt catttgaatt tatccagttt
    14341 ctatttgtct gcaattgctg aaaattaaag gagcttattt ttaataatgg aacaaattat
    14401 cactgaattt attagccgtt ttgggtatgc agccattttt atattaattt tattagaaaa
    14461 tgtactacct atcgtaccat cggaaattat tctgacattt gctggtctta tgtctgttaa
    14521 atcacattta tcaattttaa ctttatttat tattgcaact atcgcatcgt ttatagggct
    14581 gttaatttta tattatatct gccgtttgat ttcagaagaa cgtctatatc gttttattga
    14641 tcgacacggt aagtggatta aattgaaaag taaggattta aagcgagcaa atgattggtt
    14701 taaaaagtat ggcgtatggg ctgtatttat ctgtcgtttc atacctgtat tacgtgtatt
    14761 gattaccatt ccagctggtg taaaccgcat gaatgttgtg acatttaccg ttatttcatt
    14821 aataggtact acaatctgga atttcggttt aattttacta ggacgcactt tgagcgatag
    14881 ctttggtatg ttgatgactg gtcttcatac atattcacgt attatgtatg ttgtcatcat
    14941 tatcgcagtc atttactttg ctatacgtta cattggcaaa cgtaagagag taaaataata
    15001 ttgttgttag cataaaggca tgaattttaa atttctataa acaaaaagca gtgaatcatt
    15061 ccatctattg aaatgattca ctgctttttt aagtttattt ggcatacttt agctgaaagt
    15121 caactatctt tatataaaga aatattacta ttaacatgat agttatagtt gtgaagtaaa
    15181 aggctaccaa cggctgagaa atgataccaa ttaataatgc accgacagtc attccaatat
    15241 atctcacaaa attaaataca cctagcactt tcccttttat atgttcaaac cttttagaaa
    15301 ttatagtaga cagcaaagct ggcacaatac caacacaagt acctaatata aatataatta
    15361 ctgataatat aattacattt gattcgttta accatccaaa caataacgta aatatagcaa
    15421 atccagttat agtaacacga agtatcatta tttcattata tttttttgaa aatcgcttat
    15481 aaaataaact tcctaatata acactaacag taataggtaa aaacaaaact cctgatattc
    15541 caacgcctat atcataggta ttatttaata atgttggtaa ataaactaat attgaa
//
LOCUS       DAKTIP010000047        15563 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAKTIP010000047 DAKTIP010000000
VERSION     DAKTIP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.047"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..498)
                     /locus_tag="O0305_001115"
     CDS             complement(<1..498)
                     /locus_tag="O0305_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCY0274265.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLRESGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNS"
     gene            complement(849..1907)
                     /gene="hisC"
                     /locus_tag="O0305_001116"
     CDS             complement(849..1907)
                     /gene="hisC"
                     /locus_tag="O0305_001116"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCY0274266.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(2147..3661)
                     /locus_tag="O0305_001117"
     CDS             complement(2147..3661)
                     /locus_tag="O0305_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCY0274267.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(3654..4631)
                     /locus_tag="O0305_001118"
     CDS             complement(3654..4631)
                     /locus_tag="O0305_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274268.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4853..6634)
                     /gene="recQ"
                     /locus_tag="O0305_001119"
     CDS             complement(4853..6634)
                     /gene="recQ"
                     /locus_tag="O0305_001119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCY0274269.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(6646..8529)
                     /locus_tag="O0305_001120"
     CDS             complement(6646..8529)
                     /locus_tag="O0305_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCY0274270.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LIEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREENEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(8800..10740)
                     /gene="ltaS"
                     /locus_tag="O0305_001121"
     CDS             complement(8800..10740)
                     /gene="ltaS"
                     /locus_tag="O0305_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCY0274271.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(11214..12218)
                     /locus_tag="O0305_001122"
     CDS             complement(11214..12218)
                     /locus_tag="O0305_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCY0274272.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(12202..12912)
                     /locus_tag="O0305_001123"
     CDS             complement(12202..12912)
                     /locus_tag="O0305_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCY0274273.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(13000..13206)
                     /locus_tag="O0305_001124"
     CDS             complement(13000..13206)
                     /locus_tag="O0305_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274274.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(13272..13880)
                     /locus_tag="O0305_001125"
     CDS             complement(13272..13880)
                     /locus_tag="O0305_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCY0274275.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(13880..15031)
                     /locus_tag="O0305_001126"
     CDS             complement(13880..15031)
                     /locus_tag="O0305_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCY0274276.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQT
                     SYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(15015..>15563)
                     /locus_tag="O0305_001127"
     CDS             complement(15015..>15563)
                     /locus_tag="O0305_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCY0274277.1"
                     /translation="IDYIKTQTKLTVQVVGIDNLLIEDVINMKPKAIVISPGPGNPDD
                     YPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVHGHTTQLRHTNEGIFQ
                     GLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHIRFPVFGVQYHPESILS
                     EYGYRQVELFLSKVGDYCENRI"
BASE COUNT         4839 a         2829 c         2144 g         5751 t
ORIGIN      
        1 gctatttacg cgttcaaatc gatcatcatt aatattatgc actgctgtaa acataatcgg
       61 tgtaccagta aaaatttcaa gctgtctagg attgtcatct attaaataat cagctttaac
      121 gatgtttttt ctaccacaaa aaacaaaatg ctgaggatct aaaaatggaa agaactctag
      181 taaccattca tatttatcac taaatgatgt tggtacatcc attgctgctg tagcaatata
      241 tacatcataa tgttcagtta attttttcac aacttcttgt gcatacggca tcactttaag
      301 atgtctgaag aagcctgatt ctctcaatac ttctgtaatt aatccatcat gttcaggaat
      361 aacatgttta agtttttgac cattcaaagc ttccatttta atacctaaat ccgctctaaa
      421 attgacagca tcaatgattt ctcctaatgt atctgccaat acttcatcca tatcaatcgc
      481 gattgattta cgggtcattt taatcttctc ctcaaaatat cagtattgat tcagtatagc
      541 aaaaaagcaa ttgatacaaa acaatatcat ctattgttgc tttttacact gaatactctc
      601 acctcatcat tcacactgca ttgagcttga gacataaatc aatgttcaat gctctacaaa
      661 gttatattgg cagtagttga ctgaacgaaa atgcgcttgt aacaagcttt tttcaattct
      721 agtcaacctt gccggcgggg ccccaacaaa gagaaattgg attcccaatt tctacagaca
      781 atgcaagttg gggtgggacg acgaaataaa ttttgcgaaa gtatcatttc tgtcccactc
      841 cccacttact attcgtattt aaagtttgat aaaacttcta acattttatc attttgttct
      901 ttaaaaccaa ttgtaattct aacaccagtt ggaaatggtc gcgtaataca ccctacattt
      961 aaaagtgctt cataaagttc atttacccgc tttgttttta caaatataaa attcgtttga
     1021 cttggcaaga aatactcact ttgaggtaat tgataaaatc tttcgcgttc aacactattt
     1081 ttatgtgtta cctcttttaa atattgttga tcttcaagtg ctgcaactgc tgcgtattca
     1141 gatatacgtg tcacattaaa tggtggtcta atgatgttcc atttttcaat cgcatgttca
     1201 cttgctacca catatcctac acgtaaaccc gctaatccat acgcctttga aaatgtacgt
     1261 aataagaaag cattgtcata tttttgttgc aaagcaagtg tatccgggta gtcctctgct
     1321 gtcacaaatt caaaataagc ttcatctata attactggta catgtggagg tacttgagat
     1381 aaaaacgaat ctaagctctc atgattaaaa tatgtacctg taggattatt tggattacat
     1441 aaccatacca atgacgtatc ttcattaact tcttttaaaa taccttctaa atcgaagcca
     1501 ccatctttta aaggtacttg tatcacatta gctgattcaa caatcgcatt gtgataatat
     1561 tgaccgaatg tcgcttcact tgtaacaata gtatcccctg gcgttaatac agctctagaa
     1621 atcattaata taacttcatc taatcccgca ccaaataaaa tgcgtgattg atctacattt
     1681 aaatgtttac taatcgccgc ttttaatgtc ggtgatcctg tttcaggata ataatataac
     1741 tcatctaagt gtgctgatat cgcttcttta actttaggcg atggtccata caaattttca
     1801 tttgatgcaa gtttatataa atctccttca atgccatact tttctttcaa tgcccttgga
     1861 gataaaccag gctgatatgc tgatagttga ttaagttgtt ctttcataat aaaaacaacc
     1921 tccttagaat taactgaaat ttcaaaaaac ttatattcat tatatcgctc tatgtttcta
     1981 aaatgcaatg ctactatttt cacatcataa tagcttacat tgtgtttaac atgtcatatt
     2041 ttttaatcat tattgcatgt ttaaattgaa cataattata gatttaaata taacaacgcc
     2101 acataagata taatttggtg ttacgcatct tatgtggcgt tggtcgttat ttgattaacc
     2161 cttttgcttt taaataatct ttcgcaactg tatatgggtc ttcattttta actgttactt
     2221 tatagttcat catttgcata tcttcatcag atattttgtt ttctagtttg tttaacggtt
     2281 tcttaatttc tggatgtttc tttaaaaagc tttctttaaa taatggtgct ccttgatatg
     2341 gtggaaatac gtgcttatca tcttttaaca caaccatatc atattgtttt aattcagcgt
     2401 cagttgaata tgcatctatt aaattaatat tacctttatt gatcgcttga tatctcaact
     2461 taggttccat tgtacgtatg ttatctaaat ttaaattata agccttttga acagctttgt
     2521 aaccatctgg acgatcatta aattccaatg taaatcctgg tttaagttga tctttaacct
     2581 tatttaaatc accaattgta cgtatattat gttgtttagc aaaatcacgt tttacagcta
     2641 aagcatacgt attgttatac ttcattggtt ttaacatagt catttgatat ttcttttcaa
     2701 gactttgctt agcttgttca taaacttttt tctcttcttt tgacttcaat ggttcttttg
     2761 ttaattcacc taaaactgtt ccagtaaatt ctaaataccc atctatatcg tcagatttta
     2821 aagcattaaa taaaaatgct gttttgccca taccatcttt cacttctaca gtatttttgg
     2881 tctcttcttc tattaaaatt ttatacatat ttgtaataat cgatggctcg gagccaagct
     2941 ttccagctaa cgtaatttta tcaccttttt gtgcaaacat aggaatagcg atagccagta
     3001 taataatcat cactataaca cctaacgtca tcaataactt tttataagat aatttagcca
     3061 taaatcttaa aattaaatca aatataattg ccaataaggc tgctggaatt gcacctaata
     3121 atatcaacga tgcattgtta cggtctatac ctaataaaat taaatctcct agtccgcctg
     3181 caccaattaa tgctgctagt gttgctgtac ctataattaa taccatagcc gttcttacac
     3241 cagccattat aacaggcatt gctatcggaa gttcgacttt agttaaacgt ctaaatggtt
     3301 tcatacctat acctttagcc gcttcaatga gtgatggatc aacttcttta attccagtat
     3361 acgtattcct taaaattggt aacaacgcat acactacaag tgcaataatt gctggcacac
     3421 gaccgatacc aaataaagga atcattaaac ctaataatgc caacgatggt atggtttgaa
     3481 gaattgccgc aatattcatt acgatttcag atatcgtttt agtcttcgtt aataaaatac
     3541 ctaatggtac cgcaatagca gttgcaatca ataatgcgat aaatgatatt tgaatatgtt
     3601 ctatcattgt cgaaaagagt tgccccttac gttcactcaa tatgtcgaaa aagttagtca
     3661 tgttgagcta cctccttttt ctgggacaaa tatttgaaga tatctttcct atcaataaca
     3721 tattgaccta cgctatcttc ttgcatgaca atgacacgct cgctctctga taaaagttga
     3781 tacaatactt caattggttg attgtcataa acaattggat aagcgctcat agatgtaacc
     3841 tcatcgattg gtttcataat atccaagtca cggataattg cgttctcttc aacacatggc
     3901 gcatcatctt ctaaatgact acccataaat tgtttaacaa attcactttg aggattattt
     3961 ttaaatcctt ctggtgtgtc aatttgttca atatgccctt cattcaaaag acaaatctta
     4021 tcaccaagtt tcatcgcctc ttgaatatca tgtgtaacaa atatgattgt cttcttaatt
     4081 ttagtttgta attcaattaa atcatcttga agtttttctc ggctgattgg gtctaatgca
     4141 ctaaacggtt catccattaa aataactggt ggatcagctg ctaacgcacg tataactcct
     4201 acacgttgtc gttgcccccc tgacaattca tcaggttttc tgtttttata tttttcaggt
     4261 tctaatccaa ccatttcaag taattcatct actcttttat ctatatcttt ttctttccac
     4321 tttttcattt gtggcacttg tgcaatattt tctttgattg tcatatgtgg gaataatgca
     4381 atctgctgca atacgtatcc aatatcccaa cgcatttcgt atactggata atcacttatt
     4441 ggtttatctt taaaataaat ataaccttca cttaagtgaa tgagtcgatt aatcattttt
     4501 aatgtcgtag tttttccaca acctgaaggt ccaattagca caaaaaattc accctcatta
     4561 atattgaaac taatgttatc gacagcaaca tgtttgccat aacgcttagt tacattttta
     4621 aacttaatca ctttgccacc tctttttttc tcatagcata aaaccgagat tatatgtatg
     4681 tattccctat ttaaccacgt ttattacaat tttcaaattt aaatgattta tccttgaact
     4741 tttttaataa aataatgaat aataggtaat ctccagttaa gaaatagtgt tattttacct
     4801 tgaattcaaa aaaacaccca gtaaaacaag gaatgcttac taggtgtctt cactatactt
     4861 tggctttata attttgaatc gtttctaaaa atgctggaca ataatgtttt aatttgtaac
     4921 tacctacgcc atcaatatta atcatatctt gtttcgaagc aggcttacgt ttagcaaatt
     4981 cctccaacgt gtaatcagaa aatatactta caggtgctat cgttaatttg tcacttaact
     5041 ttttacgaac ttctaccaac tgactgaata atactcggtc aaccccttca accgtattta
     5101 taaatacttt ttcagtcgct ttttgcttaa atggtgttgt gaatacttct acttcattac
     5161 tgagtaattt tttaattgaa gtatcacaca ttaatatttc gtcattttca tttaagaacc
     5221 ctttgaatct taattcatct attaagtgac ttaattctga tgttgtgtaa cctttcatta
     5281 aaccatgggt tgaaatttgg tcataacctt tatacttaat ataatctgtt gactctcctc
     5341 ttaacacttg aatgataaca ctataactct cttgttgttt catacgagcg atgcaactaa
     5401 taatcatctt agcttcttgt gtcatattat atgatttatc ttgttgaaca caattactac
     5461 attgttcaca ttcttctaat ttttcattcg gttcaaaata atggacaatt gttgcttcta
     5521 gacatttttt tgtttttgta tattgaatca ttttagttaa cttttcgccc attttatctt
     5581 tatagtcatc atcagcttga gagactgtta taaaatactc gtgtaaattg atatcgcgtt
     5641 cgctaaataa caaaatacat tcacttttca acccgtcacg acctgcacga cccgcttctt
     5701 gataataaga ttctaaatct ccaggcatat tataatgaat aacaaagcgt acattggatt
     5761 tatcaatacc cataccaaaa gcatttgtag caacgactac tttaacacga tcaaataaga
     5821 aatcattctg cgcttcttct ctttctttat tgctcaaacc tgcatgatat ataacacttt
     5881 caattttctg actttctaag gcttcttgaa gctcttcaac ttgcttacgt gtagaacaat
     5941 aaataatacc tgcatcttca tcgtgtgttt taatataatc caatataaat ttttgacgtt
     6001 gataagtagg atttacttta aaaattaagt ttctacgctt agtactcgtt ttaatttgat
     6061 cagtttgagc gatatttaac ttttctctaa tatcttgctg tacttcaacc gtggcagttg
     6121 ctgtcaacgc tattattgta aaatcttgag gtaacgtaaa tacttttgaa ataacatttt
     6181 ggtaactcgg cctgaaatca tgaccccatt tagaaataca atgcgcttca tcaaacgcga
     6241 ctaagtgaat ctttatacgc tgaagcatat ttaaaaaata tcggttttca aatcgttctg
     6301 gtgcaacata caaaaattga atttctccat ttgataatgc tttttcaata cgttgttgct
     6361 ctttttgagt caaactacta tttaaaaaag cagcttgaat tcccatcgct tttaattgat
     6421 ccacttgatc tttcattaat gatattagtg gacttattac aattgttgta ccacctaaca
     6481 ataaacctgg tacttgatag catatagact tacctccacc agttggtaag acaccaagca
     6541 cattacgatg gtctaatact ttgctaataa tttcttcttg tcctggtcga aacgtttcat
     6601 acccaaagta atgcgataat gtttgttgca tcataaattg accccttatt gttgttcttt
     6661 aatttcttct aactcactcc atcttgtgat gtctaaatca tattgaattt caagttgttc
     6721 tttttcttcg tttaattctt taattttccc ataatctgca cttgcctcaa tcatgagcac
     6781 atcaatttct tccattctta cttccgcttg ttctatgcgt ttcatcaatt gttcatattc
     6841 taatttttct ttatatgata aaccattttt cttacgtaca gttgtagaag atttagattg
     6901 ttgcttcaat gtggatttat ttttatctaa tgatttttta taactttcat aatcttcaaa
     6961 agttccgata atcttttcca tctgaccatc atgaataaac caatatgact gtgcaacttt
     7021 atttaagaag tagcgatcat ggcttacggt aatcactgta ccaccgaaag tatgaatata
     7081 atcttcaagt attgttaaag tctcagtatc taaatcattt gtcggttcat ctaacaacag
     7141 aacatttggc tggtgtacga gtagacgtaa taaatacaaa cgcttttgct ctccaccaga
     7201 taatttataa acttttttac catgagttgc acttggaaat aaaaatcgtt caagaagttg
     7261 tgtaatagat accgaggttc catctttttc ttttgcgatc tcattttctt ctcttaaata
     7321 atcaatcata cgaatatctc tatccagggt ctcatctgtt tgcttaaaat aagctacttt
     7381 aaccgtctgc ccagtcttca atttaccttc gaattgttgg tcttctccac tcaaaatatt
     7441 taagagtgtt gttttaccag ctccatttgg cccaacaaca ccaatacgct cacctttttg
     7501 aataatttcc gtcagatgtt caattaatac tttatcatta atagcctttg ataagtcttc
     7561 taattcgaac acttgcttac ctaatcttga ataagcaaga ttcaattcac ctttatcgtc
     7621 tttatattgc tggttaactt cattttctag gtcattaaat cgattaattc tagcttgttg
     7681 ctttgtagta cgagccttag ctccagccct catccaagca agttcttcct tatataaagc
     7741 tcgttgcttt tgttgttgct tttgaagtgt tacttctctt tcagcgcgca tttcaatata
     7801 agattcatag ttaccaggat atgacgctaa cttacctctg tttagttcaa taattctagt
     7861 ggaaacttca tttaaaaaat atcgatcatg ggttacgaat aaaacagtat gaggatattg
     7921 cttcacataa ttgatcaacc agctgattga ttcgaagtct aaatggttcg taggttcatc
     7981 taataacaat aaatctggtt gttctattaa tgttttagca agtacaacac gtttttgttg
     8041 tccgccggat aattctttaa tgtatttagt agtatcatgt atacctagtt ttgagaggat
     8101 tgttttaatt tcagcgttat agtcccaagc atcatgttga tccatagcat cttgcgcatc
     8161 catcattcgc ttgaacaatt tatcactttg gtcatccgca taagcttgta ctgcctgctc
     8221 atattgcttg atgatgcgta aagttgttgt atcagaactt aatactgcat caaaaactgt
     8281 catatcttca ttaaggtcct gtttctgaga cgaatatcga attcgatatt gatttggatg
     8341 cataacattg gctgtaaaat catcatcaat accaccaatt acttttaaca acgtactttt
     8401 ccctgtacca tttatgccta ctaaacctat tttttcacct tctgaaattg ataaatctag
     8461 gttatcgaat atagtcttat cggcataaga tttatttaaa tgttcaattt tatatgcttc
     8521 catacccatc ttccttcttc tgttgaaatc tctacgtact taaatcagtt ctatacatta
     8581 tacacctttt tgaaatcaaa agaaatcgat accgttaagt gtcataattt aatatattgt
     8641 tgcttcaact ataaataaaa cttgttaagg ctttgatgac tatgctaatg atctttataa
     8701 tatacctttt tatcaacaca tataatgtaa aacataatat aaaagaacat aataaaaacc
     8761 cggattgata ttttatcaat ccgagttcgt gtttagatat tattttttag agtttgcttt
     8821 aggtcctgtt tcatatttat acttcgaagg atttaccttt ttgaagtctg gatttttgta
     8881 gaatctaaac aaatcaccat taagcacgtt gtcactcatt tcgagatcct tttcaacttg
     8941 ctttttattc ttttcgaaat cagctggttg agtagttatg agttcattat ttttattaga
     9001 ataaatctta ccattaacat atttataatc ttttgttata aagtcaccat ttctgaatgg
     9061 aactacttga ttatgacctt tagagaataa atcagtaccg aacattaaat agttcttcgt
     9121 atctatacca gccaaatgta aaattgttgg cattacatcg acttgaccag catattcatt
     9181 attgatacca ccagatttac cagggatttt aatccagaaa ccagttctgt ttaaatctgt
     9241 aaatttagcc ggtgtgattt tttcacctaa tagtttttcc atggcattgt tatggttttc
     9301 agagatacca tagtggtcac cataaatcat aatcactgaa ttgtcatata atcctttttt
     9361 cttcaagtca ttaatatatt cttctaatgc ttcgtctaaa taacgtgctg tttgaatata
     9421 accatcaact gttgcatcac ctgtgtttga tttctcaata gttgcatcct tttcatctaa
     9481 tgtgaatgga tagtggttag tcaatgtaat taaatgagaa tagaatggtg atttcatctt
     9541 agcttgataa ttagcagaat ctttaaagaa aattttgtct ttcaagccta agtttacaac
     9601 gtttttatct gacatgtcat agtatgttgc atcatagaat ttatcgatac caaagtgttt
     9661 atatacttgg tctctgttcc agaatgtttt atagtcaccg tgcatgacat cagatttgta
     9721 gccttgcttt tgatctaaaa ttgctggtaa tgactgatac gtattatctc cttttaatga
     9781 aaaggcagaa ccttgcggta aaccgtataa actgttatcc attgtaaatt cagagtcaga
     9841 tgttttacct tgacctgttt gatggaaaaa gttagggaag tatgtgaatt gctctttccc
     9901 acttgataat ttgtttaaaa acggtgttac ttctttacca ttaacctttt tattaattaa
     9961 gaaggtttgg aaactttcta aatgaatctt aataatattt ttcttctttg ccaccccata
    10021 atattctggg ttaggctctg tttgacgttg tttcgtataa tttaatactt ttgttaagtc
    10081 atcttcagat gctagcgctt tttgttgatt attttcgata gttttaacac catcgtatac
    10141 tgtaaagtta taaggtccta aatatttcac taaatattta tggtcaaatg tacgtgttaa
    10201 taattctggt ctgtcagttt cagcaaaagc taagtttaag aagaataatg ctactgaagc
    10261 cgccattacg acaggaacaa atttcttact aaatgctttt gtgtctaacc attttgtttt
    10321 aaatattaaa atgaataagt aaactaacgt atcaatgaaa taaacaaagt cataccattt
    10381 gaatgatgca ctaaccgcac cacccataga ttctacgtta cctacttggt ttaaagtact
    10441 aaacgttaaa aaatcagaga agaatctaaa gtacacaaca ttggcatata ataggaacgt
    10501 caataagaag ccgcctatga acatgaacca aaatgctttt ttgcctttaa agaataggaa
    10561 cacacttaaa accagtgcta ctaaactata aggattcatc aataatatta agttttgtac
    10621 taaaccttta acacctaaag aaaaatcaac ataataagaa aaatacgtct tcaaggtaat
    10681 cgttattacg gttaataaga agaacgcaaa aagactaatt ttcttttttt gtgaactcat
    10741 gattctttcc cccgttattt agataataaa tcttgcgaaa gtacttgaat aaattagata
    10801 ttaacattat gtagttaatt aataagtgcg tttaaaagtt aacattgtca taattatagt
    10861 aattactttc agattcaagt ttataatttt cgttatttgt agctttcaac tttgatttaa
    10921 caagtatttt gggctacaac tcatatttgc ttgtgtaagt ttctttattt aatattatca
    10981 ttcaattaac aaatttaaaa tttgtaatca aattgtaata ataagcatag ctcaattaat
    11041 aaatatagag taattttagt caaaattcaa gtaaaaacac actttatctt ttcattttac
    11101 tgtgaaacga attagaaaat gatacatttc gcagataaaa cacagtatta tttcattata
    11161 cattaggagg aaattatttg catcggactc gagtatgagc tacgtaccaa atattatttc
    11221 atcatttcta attgcattct atattctatt ccatggaata attggtttaa ccagttacta
    11281 tacttaacaa ggttcttttc agccgttaaa atatctataa attgaaattt caaacgtgat
    11341 ccttgcggtt tttgtgctaa tttcgttaaa tgataacttg cgattgtacc gatttgcggg
    11401 tagctaccta gcgtataatg atcatttaat aggataatag gtgtgccatc acgcttaatt
    11461 tgtatcgttc cttttttgac agtctgatac ggtggcatat cttcataaaa agctttgatt
    11521 ttttcacctt caagcatcat ccccatgcga tttgctttgc tcgttacttt ataatcatgt
    11581 ttcacaaatc tttgaatggc atcttcttta aaatcttccg tacctttatt tttaacgaca
    11641 tgaaatacat cagacatata attaaatgac aaggcgtatc catcaatacc ccaatctgtt
    11701 tgtttcgtgt gagcaaggtt ttcaaataac ttatgatgac gagctgtata atctctctta
    11761 agctttattt catcgccatc ttgtaatgtt ctacctttaa aaccaccaat ttttacatta
    11821 aagtcggttg agttagatcc taaccatgca tctaattcaa atccgcctcc cacagctaaa
    11881 tacactcgcg atgtataact tgtttctcta aactttaaaa catcgccttt atctaacaaa
    11941 tacaatttat atggagatat agtcatatgc tcagtgtaag ctttgacatt accccctgct
    12001 aatgcaatca gcgtaggttc tgtaaaacga atcgttgcca ttttattagt catttccaaa
    12061 gttgcttcat tcttgtcatt tgcaactaat cgattagcaa tttcatgtgc taaagtatca
    12121 agtgcaccac atggaattac accatcatgt tcatatcccc ttctgccaaa gtcctgaaag
    12181 ctactgaata agccactttt ttcaattatg attgacatct ttaaacgctc ctacttctaa
    12241 ttcattttca ttgataggtc taaatttaat attatcgcct aactttaagc gtgcgaaatc
    12301 tgattctttc ggattaaaaa gtgataatgg tgtataacca ataactaacc aatcattaat
    12361 tgtatccgta gttacaatac cgcatttttt cccttcaagt actacagaac cagctggaat
    12421 gaattttttc tgtttactcg tgtgattaat atacaatttc ttatttaatc cagttaaata
    12481 aggaaaccca ggtgaatatc ccatcatcga aacaaaatat tgcgccttag aatgtaattc
    12541 aataaaagtt tctagcttga ttttgtaatg ttttaaaagt gattctaaat ccggaccata
    12601 tttcgaacca tacacaattg gtatttcaat caaatcttgc tggttaaaat catgttttat
    12661 gtttaaatga atcgattcta ttagtgcttt catgtataaa aaaggtgatt gtatattatg
    12721 gtgtttaatc atatcccttg cgtcatagac aatcatcatg tctgattctg atggcacaat
    12781 ttctataata aatggataat tttgttcaat taaatatgag cgaagtgtta ataaatcttc
    12841 ggttaaactt ttagatactt ctttttcaat tgcgactaca atggcttggt caccttgact
    12901 atatattttc atttgtacac ctcttcaata tttggttact ttactttcat ttttaattat
    12961 agaatcgatt gaatagttca ttttacctac tcactaaatt tactggtggt acattaaatc
    13021 cattgcccgc acaacaaaag gcataagttc gtgaattaat gcgtatacaa ggataaagct
    13081 tataacagta gtaattgttg ctatcaaacg aacaacatat attctatttt cagatagcaa
    13141 tcgattcact aatcgataat ttacgtatac aagaattaac aacagcacaa tataaacaat
    13201 aatcatattt ggccccattt aatttttaaa tttgttttca catcattttc tactttatta
    13261 tcatactaat tttaaaggca aaggtggaca tcggcaacct ctcgtaaact atttatcaaa
    13321 aataaacgta tctcattgtt atgatattta taaatcaatt cgtttttatt aaagtctttt
    13381 tcaacaagtt tgtcactatc tattaaataa tcacgcatgc atccttttaa gaaatcatct
    13441 ttataacttg gtgtgtacca ttttccatcc tcttcaatga caatgttgcc aatatcaaat
    13501 tcaaggacct tgccgtcctc tgaagttagc aatattaaat ctgtttcatg attgtgtgct
    13561 aaatgctttc gttctgtcgt tttatttttt ataaaagttg gattaactac tttgggcaac
    13621 acttgaaatt ttgctgtaaa actacttttc tcaggtaaag gtgacactat cgtttcaaat
    13681 ttgctttcag catttaatac gattttaagt ctatattgac cactgtgata ctttgttgtt
    13741 acatcgttta attcatttcg ccatgcatgt tcatcaaatt taaagtttaa tcgctcagaa
    13801 gaacatttta tgcgattagt atgataagta agtctaggga tatgtccatt atcaattttc
    13861 attgtttcaa ataattgcat tataacatct ccaaaatctt ggtttttgcg taaaattcat
    13921 tcacttcatc ttttggctta gaatcaattg taatacctgc tccgactcca taaatcgctt
    13981 gtccatattt atactcaata gtgcgaatcg gaatattaaa aatcatttta tcatcttcag
    14041 ttggaagtaa tagtccaatt gctccgcagt atataccacg aggtgaactt tctaattgtt
    14101 taatatattt cattgtattc agtttcggtg cacctgtaat cgaaccacaa ggaaataacg
    14161 atgttaaaat ttgagttaag tctgtattat tttttaaagt tccacttacc atcgaagtca
    14221 tttgaaatac agttttatat gcctcaataa aaaatagttt atacacctta attgagccac
    14281 tctgtgatat tctccctata tcgtttctta gtaaatcaac aatcatgaca ttttctgcac
    14341 gatctttcga agaagtttgc aatgttttat aatactgttg atcttcagct tccgttttac
    14401 ctctaggcat tgtccctttc atcggtttgc ttatgataac gttatcgaca ttgttaaatt
    14461 gtcctttttg aaaaaataat tctggtgaga tagacgctac ttggatttca tcagtttgta
    14521 ataacgcagt ataattacca ttactaaatt gagttaatcg ttcatataaa gtactaatag
    14581 gataataaat gttatccgtt aagcgcgccg tatagttcac ttgatacgtt tcgccttcaa
    14641 caattgcttg ttggacacgt ttaatattag tcatcataac ctcagaagat tcaacaaatg
    14701 aaaaatgata ctttgataca taagaagttt gatgttcata tgttgaattt atgctttccg
    14761 ctttttcaaa actataagct gctgcataaa tatcatcttt agctaatgaa tgtgtacaca
    14821 tagcatgatt aaaatacttt gccgcttcgt aacttaaata taacgagaca tatctacctt
    14881 gtcgttgctg cgcttgtgca aagtgtatca cttctcccac atcagccaac ttagtagcaa
    14941 catacttctt tataaatccc tttaattgaa tatggtattg cttatattca ttttcagtta
    15001 aatagtagcg ataattatat tctattctca cagtaatcac ctaccttcga taaaaataat
    15061 tcaacttgtc gataaccgta ttcactcaaa atagattcag gatgatattg cacaccaaaa
    15121 accggaaatc taatatgctc aaatgccata ataatcgctt catcgttttt tgctgtaatc
    15181 tttaagcaat ttggaaaagt cgctccgtca gcaattaatg aatgataacg cattacattg
    15241 aaattttgag gcagtccttg aaaaatacct tcattggtat ggcgtaactg tgtagtatgt
    15301 ccgtgtacag gatgatagcc gtgaatgata tttccaccaa aataagacac gatacattga
    15361 aatcctaaac atacacctag tataggtaca cgctgataaa attgttctaa cacttcattc
    15421 aagataggat aatcatccgg attacccggc ccaggcgaaa taacaattgc ttttggcttc
    15481 atattaatga cgtcttctat cagcagatta tcaataccaa caacttgaac tgttagtttc
    15541 gtttgagtct taatatagtc tat
//
LOCUS       DAKTIP010000048        15342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAKTIP010000048 DAKTIP010000000
VERSION     DAKTIP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.048"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            142..1497
                     /gene="brnQ"
                     /locus_tag="O0305_001128"
     CDS             142..1497
                     /gene="brnQ"
                     /locus_tag="O0305_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0274278.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            1750..2934
                     /gene="rocD"
                     /locus_tag="O0305_001129"
     CDS             1750..2934
                     /gene="rocD"
                     /locus_tag="O0305_001129"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0274279.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            2973..4004
                     /locus_tag="O0305_001130"
     CDS             2973..4004
                     /locus_tag="O0305_001130"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCY0274280.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            4016..5257
                     /gene="argJ"
                     /locus_tag="O0305_001131"
     CDS             4016..5257
                     /gene="argJ"
                     /locus_tag="O0305_001131"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCY0274281.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            5273..6037
                     /gene="argB"
                     /locus_tag="O0305_001132"
     CDS             5273..6037
                     /gene="argB"
                     /locus_tag="O0305_001132"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCY0274282.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            6307..6786
                     /locus_tag="O0305_001133"
     CDS             6307..6786
                     /locus_tag="O0305_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCY0274283.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(7114..7758)
                     /locus_tag="O0305_001134"
     CDS             complement(7114..7758)
                     /locus_tag="O0305_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCY0274284.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(7771..14946)
                     /locus_tag="O0305_001135"
     CDS             complement(7771..14946)
                     /locus_tag="O0305_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCY0274285.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKVKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNQDASHIALDSNYFRLENNSDIHIDSYFPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINLSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     VTPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIAVEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPETIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSE
                     LNVAQLRQAVQRLIARHEILRTQYIVVDDEVRQRIVADVAVDFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPEQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDQLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGATGFLGAYLIEVLQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEIMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTNPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
BASE COUNT         4897 a         2892 c         2431 g         5122 t
ORIGIN      
        1 cataaaaaac agtatttatt ccggaatttt caaaaaattt agtatttatt gcaaaattat
       61 gtatcacttt atgtttaatt tttgatatta tcttaattaa gtagattttt ataagttcta
      121 aaaaggagaa caaatacata tatgaagaag aaactaacat ttaaagaaaa catgtttata
      181 ggttctatgt tatttggttt attctttggt gccggcaatc ttatcttccc aatacacttg
      241 ggtcaagctg ctggttctaa cgtttttatc gctaacttag gatttttaat tacagcaatt
      301 ggcttaccat ttctaggtat cattgctatt ggcatttcaa agacatctgg tttatttgaa
      361 attgcatcgc gtgttaataa aacatatgct tacattttca cgattgcctt atatctagtt
      421 atcggaccat ttttcgcctt acctagactg gcaacgacat catttgaaat tgcattttcg
      481 ccatttttat caccaaagca aatcacttta tatttattta tttttagctt cgtcttcttt
      541 gtgattgcat ggttttttgc gagaaagcca tcaagaattt tagaatatat cggtaaattt
      601 ttaaatccgg tattcttagt attattagca attattttat tatttgcttt tatccatcca
      661 ttaggtggca tatctgatgc acctattagt aaacaatatc aatcacatgc cttatttaac
      721 ggctttttag atggatacaa taccttagat gcgctagcgt cattggcatt tggtattatc
      781 attgttgcaa cgattaaaaa gttaggtatc gaaaatccaa ctgatatcgc taaagaaaca
      841 attaagtctg gtactatcag tatcattatg atggggatca tttataccct actagcaatc
      901 atgggtacat taagtattgg tcatttcaaa cttagtgaaa atggtggtat tgccttagcg
      961 caaattactc aatactactt aggtaactac ggtatcgtcc tgttgtcact tatcgttatg
     1021 gttgcttgtt taaaaacagc catcggtttg attacggcat tttcagaaac attcgaacac
     1081 cttttcccta aaatgaatta cctagcgatt gcaacagttg taagctttat ttcgttctta
     1141 ttcgcgaatg ttggtttaac taagattatt atgtactcag tcccagtgtt aatgttctta
     1201 tatccattag caattgcctt gattgtacta acattattta gtagcaaatt ccatcattca
     1261 aaacttattt atcaatgtac cattttcttt acaatgattg ctgcattagt agatggatta
     1321 aaagctagtc cagagttcat ttcaagcaca tcattctcac aaactttgat taatttcagc
     1381 caaaaatatt taccattatc agacattggt atgggctggg ttgttctcag tttgattggt
     1441 ttcattatcg gcttcattat ttataaaatt aagcatcgta aaattccaca agcataatac
     1501 tatgccacag tcatatgtta aacatatgct tgtggcattt tttattcata ctacattaaa
     1561 ctgcaatcgt atacatacat atcaatgatt atccacaaaa aatattagta ctttcatttt
     1621 acaaatcaca ttaatacaaa cacaacctta tctttatatt attaaattta catttgacac
     1681 ttatattgaa caactgtaat atattaatat taattcttta aaatgtataa atataaagga
     1741 gggagaccga tgaattcaat cattgaatta actgattatt atagctctaa taattatgca
     1801 ccacttaagc ttgtcatttc taaaggtaaa ggtgtcaaag tttgggatac tgatggcaaa
     1861 caatatatag attgcatttc gggtttttca gttgcaaacc aaggccattg tcatccaaca
     1921 attgttaaag cgatgacaga acaagcttca aagttgtcta tcatttcacg tgtcctttat
     1981 agtgacaatc tcgggaaatg ggaagaaaaa atttgtcatc ttgctaagaa agacaaagta
     2041 ctccccctta actctggtac tgaagctgtt gaagcagcca ttaaaattgc tagaaaatgg
     2101 ggctctgaag ttaaaggcat tactgacgga caagttgaaa tcatcgctat gaataacaat
     2161 tttcacggtc gtacacttgg ctcattatca ctatctaacc acgacgcata taaagcagga
     2221 tttcaccccc tacttcaagg cactacaaca gtagattttg gagacattga acaattaaca
     2281 caagctattt caccgaatac agcagcaatt attttggaac caattcaagg tgaaggtggc
     2341 gttaatatac caccgaaagg atatattcaa gctgtgcgtc aactatgtga taaacatcaa
     2401 atattattga ttgcagatga aattcaagtt ggtcttggta gaactgggaa atggtttgct
     2461 atggaatggg agcaagtcgt tccagacatt tatattttag gtaaggcatt gggtggcggc
     2521 ttataccctg tatctgctgt acttgcaaat aatgatgtca tgcgtgttct aacaccaggt
     2581 acacatggtt caacatttgg tggtaaccct ttagccattg caatatcgac ggcagcgctt
     2641 gatgtactta aagatgaaca actggttgaa cgatcagaac gcttaggttc atttttatta
     2701 aaagcgttgc tacaacttaa acatcctagt attaaagaaa ttagaggtcg tggtttattt
     2761 ataggcatag agcttaacac agatgctgca ccttttgtgg atcaactgat tcaacgtgga
     2821 atcttatgca aagacacgca tcgtactatc attcgattgt ctccacctct agtcattgat
     2881 aaagaggaaa tccatcaaat tgttgcagct tttcaagacg tttttaaaaa ttaacaatta
     2941 atcatttata tatgacatag gagggattca tgatgattaa agtaggtatc gttggcggta
     3001 gcggttatgg cgcaattgaa ttaattcgat tgttacaaac acatcctcat gtaacgattg
     3061 cacacatcta ctcacattca aaagtagatg aaccgttgaa attaacattt ccacatttac
     3121 aacatattat gcaacatttc gaagcactta cagtggacaa taatgactgt gatgtaattt
     3181 tctttgcgac accagcaccc gtaagtaaaa catgtatccc tcccttagta gaaaaaggta
     3241 ttcatgttat cgatttatct ggcgcattta gaattaagaa tcgtgaaata tatgaagcat
     3301 attacaaaga aactgctgca gcacaagatg atttgaatca tgctatttac agcatttcag
     3361 aatggcaatc gtttgataac aatggaacga agctcatttc taatcctggc tgtttcccta
     3421 cagcaacatt attagcatta catccactta ttagcgaaaa aatagtagat ttgtcatcta
     3481 ttattattga tgctaagacc ggcgtgtcag gtgctggtcg ttcattatca caacgtgttc
     3541 atttttcaga aatgaatgaa aatctaagcg cttatgcaat cggaaaccat aaacacaaac
     3601 cggaaatcga gcaatattta tctatcattg cgggtcaaga tgtatcagtc atatttacac
     3661 cacatctcgt accaatgaca cgaggtattt tatcaacaat atatgtcaaa ttatcatctg
     3721 aatatacgac tgaatcatta cataaattaa tgacctctta ttatgctaat cagccatttg
     3781 tcagaattag agatattggg acttttccaa ccacaaaaga agtactcggt agtaactact
     3841 gcgatatcgg catctatgta gatgaaacaa cgcaaacagc aattttagta tcagtgattg
     3901 ataaccttgt caaaggcgca agtgggcaag ccattcaaaa tttaaatata ttatatgatt
     3961 ttgaagtgac gactggccta aatcaatcac cagtttatcc ataaggggtg ttagaatgaa
     4021 acatcaagaa acgacatcac aacaatataa cttttcaatt attaaacatg gcgatatcag
     4081 tacacctcaa ggcttcacgg ctggtggtat gcacatcggt ttacgcgcta acaaaaaaga
     4141 ctttggatgg atttactcat cgtctttggc aagtgcagct gccgtatata ctttaaatca
     4201 gtttaaagct gcaccactta ttgtcactga agacacttta caaaagtcta aaggaaaatt
     4261 acaagcactt gttgttaatt cagctaatgc aaattcttgt accggtcaac aaggcataga
     4321 tgatgcacga caaacacaaa catgggttgc tcaacaactt caaataccat ctgagcatgt
     4381 tgctgttgct tcaactgggg tcattggtga atatttgcct atggataaaa ttaagactgg
     4441 gaccgaacat attaaggatg ctaattttgc aacgccaggt gcgtttaacg aggcaatttt
     4501 aacaactgat acctgtacaa aacatatcgc tgtatcacta aaaatcgatg gtaaaaccgt
     4561 tacaattggt ggtagcacca aaggttcagg tatgattcac ccaaatatgg ctaccatgct
     4621 tgcttttata acaaccgatg catcgattga atcgaataca cttcatcaat tattaaaatc
     4681 ttcgactgac catacattta atatgattac tgttgatggc gatacaagta caaatgacat
     4741 ggtattagtc atggcaaatc accaagttga acaccaaata cttagtcaag accatccaca
     4801 atgggaaaca tttgttgatg cattcaattt tgtctgtaca tttttagcta aagctatagc
     4861 cagagatggc gaaggcgcaa caaagttaat cagcgtcaat gtgtcaggcg ctaaatcaat
     4921 atcagatgca agaaaaatcg gtaaaactat cgtaagttca aatctagtaa aatcagctat
     4981 ttttggcgaa gatgccaatt ttggtcgaat cattacagct attggctaca gcggatgtga
     5041 aattgatcct aactgcacat atgttcaact gaaccaaata cctgtcgttg ataaaggtat
     5101 ggctgtacta tttgatgagc aagctatgtc gaatacatta actcatgaaa atgtcacaat
     5161 tgacgttcag cttggtttag gtaacgctgc agcgactgca tacggttgtg atttatccta
     5221 tgattatgtg cgtatcaacg catcatatcg aacataaggt ggtgttggtt agatgaaatt
     5281 tattgtcatt aaaattggtg gcagtacact tagtgacatg catccatcaa ttattaacaa
     5341 cattaagcat ttacgatcaa acaacatcta ccccattatc gttcatggcg gtggcccatt
     5401 tattaatgaa gcattatcaa accagcaaat cgagccacac tttgttaatg gcctaagagt
     5461 gactgataaa gcaaccatga ccattactaa acacacgctc attgcagacg ttaacactgc
     5521 attagtagct caatttaacc agcaccaatg ttctgcaata ggcttatgtg gtttggatgc
     5581 acagctgttt gaaattacat cttttgatca acaatatgga tatgtcggtg ttccgaccgc
     5641 tttaaataag gatgctttac agtatttatg tactaaattt gtacctatca tcaattcgat
     5701 tggtttcaat aaccatgatg gagaatttta caatattaat gctgacacgc ttgcctattt
     5761 tattgcatca tcattaaaag cgcctattta tgtattaagt aatattgcag gtgtactcat
     5821 caatgatgtt gttatacctc aattgccatt agtcgatatt catcaatata ttgaacatgg
     5881 tgatatttat ggaggtatga ttcccaaagt gctagatgcc aaaaatgcga ttgaaaatgg
     5941 ctgtcctaaa gttatcattg catcaggaaa caagccaaat atcattgaat ctatttacaa
     6001 taatgatttt gttggcacaa caatccttaa ttcataacta tgaaattaag gcctaacaag
     6061 ttttgacacg cgagatgatt ccagttcgat tatccattgc gctaaaacat ttatttaccg
     6121 ttcatctcgt taacaatttt gaatacagta cgatacaata tgagatgtaa aaaactaata
     6181 accttttaca aatttgttta tcaaaatatt ttaagttttg caaagctttt tattgtgatt
     6241 attttcacaa aatactataa tgaggatagt aaatagagag gagtccttaa gttgacgaaa
     6301 cgacaaatgg gtatattcat ttatgctgga attatcggtg gcttgttatc tggaattgta
     6361 aaattaggtt gggaggtcat gtttccacct cgcacaccag aacgtaatgc aacgaaccca
     6421 cctcaagagt tattgcaaca attaggattt agtagtgagt ttacgcatca aacatataca
     6481 ttttcaaata tggaattgcc ttgggtaagc tttattgtcc actttagttt ttctatcgtc
     6541 attgcaatta tttactgcat attagttaaa aaatacgctt acttagcaat gggacaaggt
     6601 gctgtttttg gtattgctat ttgggtatta ttccacctta tcattatgcc aatcatgcat
     6661 actgtacctg ctgtgtggga tcaaccattc caagagcatc tgtcagaatt ctttggccat
     6721 atcgtctgga tgatgacaat tgaattagtg cgacaacatt ttgtctatcg ctataaatta
     6781 aattaataca ctgactaaca ttaacgtgag ttttaaatca tcgtttgagt atgatgattg
     6841 atgctcacgt tattttatta actgacatga tatgattcca gccaacttac gtgagcatta
     6901 aagtctcaaa tgcgtcgtaa caaactatta ttttcggtaa tttcaatatt gctcagtata
     6961 tttttacctt atcacttact ttaatctcgt catgattttg aatgatgcca tcgtgtattc
     7021 acctttcatt tttccaataa aaaaacatct aacagtaaac atttaggcag tatagtttaa
     7081 aatcactgcg caatgatact gtcagacgtc atattaacta ctcaataact gaaatacaga
     7141 cactttttta taaccccagg gtgcctgtcc taagaaacat acctgtacca taaactgatc
     7201 aaaaataaat tgtttgaact tcacttcacg tgattgataa aagtgtgatt gtgtcatatc
     7261 ataaatgtcc aatcctttga ttaaaccttc accaatcaat tttgtaaaac tttctttttg
     7321 tgtccatatt tgataaaaat catttaaact acatatttga tgtgcttcgt ttgtagagaa
     7381 acacgtcact aacgtacgcc agtctaaacg ttgtgatatc ttttcgatat caataccaac
     7441 tggttcttta tcgacaacac acacgatata aggataacta tatgataagc tcacatagat
     7501 gggctgtcca tcacgattgt gttgaacaat atctgcctta cctcgtggcg aaatgtgata
     7561 atgccattca tgtggtaata aacctgtgtc atgttgaatt ccatattgca ctaaaatatc
     7621 tcccaatctg tgcatgagtt tatcttgatt gtatctatag ttgactgtac gcggtttttt
     7681 atatgaccaa cgactttgtg atattaattc ttcaatactt ttcaagttac tctgtaattg
     7741 cattacaaat actgtcataa ctttccctac ttacttattg aatattgttt tgatatattg
     7801 tgcccaatga tacagccaat tgttagttat cgttggccat ttttcactga tgtgattcat
     7861 tatttttaat gttaatgttg tatctatcat tgctagttgt tgttcacggt caacactagt
     7921 taatccaatc gtttcgtaca tgtcttgttt ctgtaaaatt tcattaaatg attcatcgct
     7981 gacgagttca atttctttgc gcttaacgca ttctaacaaa gatttcaccg gcattttatt
     8041 aggtgatagc acatggtaaa tgatttgtgg tgtgttgacc tgtgctaatg cgacaatttg
     8101 tcttgcagtc gtatccacaa aagaaaaatc tacaggcatt tcagccatgc taaccccgat
     8161 acaatccagt tgtaacaaat cattcattac cattgaaaaa cggttagtct ttatatttct
     8221 catatgccat cttccattgt aaggattcgt caaattacca acacgtacaa tccgaccatc
     8281 taagccatta tttacagctt ctaatacttt taattcacta taaaatttgc tccgtgtata
     8341 tggtgatgtt agtagttgcc ctttatagac atccgcttct gaaaatgtca catcttctgt
     8401 gtctatatca aaataagttc ccacacttat cgtagacaca tatattaacc ttgcatgatg
     8461 ttgttgtgcc aaacgtatga catcaacagt accttgaaca tttacttttt caaattcatc
     8521 atcatcacca aagtgatctg tacgagcacc tgcatgaata atcgtatcca tgttttctgg
     8581 taaaacaaca tcatccatac actcgaaatc accaacaatg acttcaatgt ttgataacat
     8641 tatttcaacc gtctcttctg aaaaataatc atttaaattc gtcatcaact tataccatgc
     8701 tatttcctca ttatcagcac gtatgaaaca ataaatgcga tgactgtatc cttgtagtac
     8761 ttcaatcaga taagcaccta aaaaacctgt cgcgccagtc aatagtgtat ttcctagagg
     8821 tcgatgactt agactatcct ctaaaatacc caagttataa cgagacataa caatcttttg
     8881 taattccgaa agattatccg gtaatgcaac taatgattgt tgattttggt acatataatt
     8941 aacaatctgt cgcacggttt tatattggta taatgtctgc attgaaatat gatggccaaa
     9001 tcgttttaaa tgcgagacaa ctaacatcgc ctctaatgag ttaccaccaa gttcaaagaa
     9061 atcatcgtca acaccgacat cattttgttt caatacctct ccaaatacat caacaaatgt
     9121 ctgctcaatt tcattagagg gttcgctata cactttatta gactgttgta taggtgatgg
     9181 atttggcaaa cgcgtagtat ccaccttgtc attcgtggtt aatggcatac aatcgatatg
     9241 cgttatagtc ttaggaatca tatacttagg cagctgatca tttaaatatt gcttcaaatc
     9301 ctgttccact tgttgctctc cgacataata agcattcaat atatcatgcg tatcaaagtg
     9361 acttactgtt acaacacaat cagatatacc acgaatagct aatattgcat tttcaatttc
     9421 atcaagttca atacggtacc cgttaacttt cacttgttta tctattcttc ctaaaaattc
     9481 aatttgacca tcagatgtat aacgtgctaa atcaccacta tgatacaact ttcctttacc
     9541 aaatggatta ttttgccatt tatcagccat taattctgga cgattaatat atcctatcgc
     9601 taaactatca cctgcaatac acaactcgcc tggcatacca ataccgcata acaaaccatc
     9661 tgacataata tacacttgga tgttagataa gggtttgcca attggaatcg tctcaggtat
     9721 caaatcacca caatgatgtg accaatacga tgtgatgact gttgactcag atggtccata
     9781 ggcattgaaa tacgtgccac aatgcttctc aatatattta acaaaggatg ccgtactagt
     9841 tgccccgcct gtaatcaact tttcaatata aaagtcttcc ataacactac acatctgtaa
     9901 cggaatcgac gcaaccgtca cacgatgctt attaatgagt tgttgtaact gttctggatt
     9961 aacacgttcc tctctatctg gaatcacaag cgtatgacca tttaacaaac aacaataaat
    10021 ctccataact gatgcatcaa aaacaatatt tgcatgttgc aaaaatactt cattgtcgcc
    10081 taattgcaat tcagttgacc atgcatgcac taaattcaac aaatttcgtt gtcgtatggc
    10141 aaccccttta ggcatcccgg tcgtaccaga tgtgtaaata gcatacatct cattatctaa
    10201 catcgctgtg ttttcaagtt gattgccatg taaatcatca tattgttcat tttcctttga
    10261 ttcaacaaag cctttagcat tttccaattg aatgactggt aatgtcgttt caatttcaac
    10321 gccgtaagac atgactgcag ttactttagc atcctccaaa attgcacctt gtcgtttatt
    10381 cggaaaatca atatcgatag gtatataaga tgcacctact ttaactgtcg ccaacatcgc
    10441 cgcaatcatt tcaaaactac gttctgtaaa caaggcaacc cgttgaccat tgcccacacc
    10501 atttgatagg agcatgtgcg caatggcatc cacatagttg cgtaatgttt catacgtcat
    10561 tgtcaaatca ttcatgacta gcgcaacatg attaccttgt cgtgagacaa cttcattaaa
    10621 gtaacttatg atagatttat ttcccgggac attaagcatt cgatcgttaa catgcgtatt
    10681 gacccaattt agaagttcct ccgtgccgtt tggtatatca caaatttgta gtgtatcttg
    10741 atgcttcaaa atataatcaa tcataatcat acattgatta cccatgtgac gaactgtttc
    10801 tgagtgatat aaatcggtat tatactcgat attgattgta tagtcatcgc gatcttcttc
    10861 aatgatgaaa gataaatcaa atttcgccgt cactgatttg ggttgaatgt gtgttaattt
    10921 actatgccca aaatgagcat gattcgtttc attgttttgt agtactaaca tgacatcaaa
    10981 taatggattc cgtgaggcat catgtgattg atctaagtca tttactaaac attcgaatgg
    11041 gtattcttga tgctcgtatg cctccaaact catttcctta acctcttgta aaaactgtgt
    11101 ccacatttta tcaggtgacg gttgccctct atataccaac gtattagcaa acatgcctag
    11161 catttgctcc gtgcctttat gcatacgcgc actcatcaca ctaccgacaa caacatcatc
    11221 ttttcgagca tatctactta acaacgtcat gaccacactc ataaagaaca taaaatcagt
    11281 aatttgatgc ttttctacat acttttgaag tagctgtctc atttgttgat tcattgtaaa
    11341 tgacatcatt gctccatttg tcgttttaat atttggtcta acatagtctg tcggtaagct
    11401 taaaataggt acttcatctt tgaattgaga taaccaatat tgtctatgtt tcgtcatatc
    11461 acgatgcgac atccactcac tatagtcttt atattgcaat ttaagtggta acaataattt
    11521 atgttgataa agtgcgttaa gatcattcat taattgtata ttactcatac cgtcattaat
    11581 gatatgatgc gtatctataa agaggtatgc atgtaaggga cttctaatgt atctcactct
    11641 aatttgactt ggcttttcca aattaaaagg tgctacaaat tggcgcatga tttcttgttc
    11701 atccgtaaaa tgcgtgttaa cttcttcaaa gtcaactgca acatctgcca caatacgttg
    11761 tcgaacctca tcatctacaa caatatattg tgttcgtaaa atctcatgtc gcgctatcaa
    11821 acgctgcact gcttgtcgca attgagctac attaagttct gatgataacc gccataaaaa
    11881 aggtacgtta tacaccgtat ctttatggtt tgatttccat aataaataca tacgcttttg
    11941 tgcagagctc agcacataat catctttaac tatagtttct ggaatcactt catagttttg
    12001 ttcttgaacc ttagcaatcg cttgtgctag ttcaaataca gttggctttt gtaataaatc
    12061 accaatttgt aatcgtttcc cagtagatgc ctctatccga ttcaccacta acgttgcttt
    12121 taatgaatgg ccacctaatt caaagaaatt atcatgaata cctacttgat tcacatgtaa
    12181 aatatctgca aaaatttggc atagcaagtg ttcggtatct gtactcggtg ctacataggc
    12241 atccgtatcg acatagtcca tgataggcaa tgccttctta tctaatttcc cattaatagt
    12301 aataggaatt tgctcaatat gcatgaaatt aactggtatc atgtactccg gtaaggtcat
    12361 acgtaattgt gatttaatct tattatgtga taatgtatgc atcgcttcat aataagcaac
    12421 gatatactga tcttgatcat gattttgaac aataacaact gctttattaa taccttgtat
    12481 acgctcgagc gcatgctcaa cctctgacaa ctcaatccta aaccctcgaa tcttaacttg
    12541 tttgtccttt cgatataaat aatctatgtt gccatcgggt aacaaacgaa cgatatcacc
    12601 acttctatac atcagctgat ttatatttga atctttgata aatttatctg ctgtcaattc
    12661 tggctgattt aaataacctg cagctaaccc aaagccactt gtacataatt ctccaggaat
    12721 accaacgcca caccgacgct cgccttgcat gatataaaca tgagtaccca gaatcggttt
    12781 accaatagga atacgatttg gaactttgtt aggtatatta tacgtcgttg taaatgttgt
    12841 attttcagtt ggtccataac cattaataat ttgaggatgc ttcggttttt gattaagcaa
    12901 atccacccac ttagcattca atacttctcc accaattaat aaatacttta acggtaccaa
    12961 tacttctatt cgttcactag caatctgatt aaataatgag gaggttaacc acatagtatt
    13021 aacgtcattt tcattgatta attgttctac cgctattgga tttaataatt gttctttttt
    13081 agcaacaatc agctttccac cattgagcaa tgcaccatat atttcaaatg ttgcagcatc
    13141 aaaggctata gttcctgata acaaaatcgt cgtctcttca tttaatggta cataatgatt
    13201 ttgatggacc aatcgaacaa tacctcggtg cggaattagt gtccctttag ggttaccagt
    13261 tgtccccgac gtgtaaataa cataagcatg atcttctaac gtgttacatt tagaaagatt
    13321 atcaatattt ttccacgcta tcttattcaa atcaatgtga ttaatatttt gtttaccatt
    13381 ttcatataaa gcttggtacg ttattacaac tttaggcgtt acatctttta aaatgtactc
    13441 ctgacgatca cttggatagt tcggatcaat tggcacgtaa gccccaccag ctttcaacac
    13501 acctatcatc gctattatca tctcaatact tttttcagct atgacagcga cacgatcatt
    13561 aggttcaaca ccatactggt ttctcaaacg gtgtgctaaa tcattcgcgc gtgcattcaa
    13621 tgtttgatat gttataaaca ctccgtcaaa ttgcacagcg acatgattcg gcgttgcttc
    13681 aacttgttgc tcaaataagg taacaactgt ttgcgcatca tctatctcag gcaaacttaa
    13741 attgatatcg tcatataatt gaatatcacg ttctgtcatc aaattaagtt catctacagt
    13801 tgttcgttta tttccatttt cttcagtaat ttgcaaataa atatttcgaa ctaagtcact
    13861 cagcgtctcg attgagagca aatcatatgc tgcactgtta tatataattt tgaacccgtg
    13921 ttgatgtgga aaaatttcaa tatccgctaa agatgtatgt gcatcatgta tttgatgtac
    13981 atcctcaata acatcattac aacaagacat catatgatga taacaatgaa aaatagtctc
    14041 tagtgaaagc gaagacttcg cgcactgtaa ttgcgacata ttttgcaaca cacatttatt
    14101 aaaatctgtt gtaaaacgtt gacatacatc ttttgcatcg attgttaacg ttaacggcac
    14161 aatatttccg tgtaaatcat ttggtaaatg tgatggtaca tgtataccta atgtgacatc
    14221 atgttgttga ctcattatat gattagctaa atacacacta acagccaacg atgccatatc
    14281 tattgatgtc atatcatcaa tcaaatacgt ttgatataaa gcttgttcaa atggatgctt
    14341 aattggaaaa taactatcaa tatggatgtc agagttattc tctaaccgaa aatagtttga
    14401 gtctaatgct atatgcgatg catcttgatt gtctttatca tcatttctat ttatatgtgc
    14461 atgctgtcga gtattgttaa taacagtatt gccacgatat gcattgcata aatcatcaag
    14521 aaaaatatca atttgactat catcgaaaat ggacacatga aaatctaata gtatatatgc
    14581 agcatcagcg aactgaaaca atttaacttt gaataaaggt gaatcattaa aatggtaagt
    14641 acttaattct tgcttaaaaa aagcttctaa atcatagttt gcggaagaag atggaacttg
    14701 ttttatctca ataaaaggca gaaattcatg aagtatcatt gttaaattgt catcggtagt
    14761 aacatcaaaa aaatgtctta tagatgcatg ttgttgcaca attgtcgata atgcatacat
    14821 cattttagta gcttcaacat ttttagcgag tttaacccaa tacgcattac ggtgtgtcgt
    14881 tgattctgta ttatttttgt atatacgaaa atattcctgt tgaaatctca aattacccat
    14941 aatcataaaa agtccttctt tcatatcata atactcatta cttactgaaa ttgcatgatg
    15001 atatgataac cgacgaaatg ttaattaact cgttatgtaa tgattaatat aaaacaccat
    15061 tcgcaacata tgagcgatat attctaccct aaaatacatc ttgtatcatc gttacaattg
    15121 gtatattttt caatgtaaat tacatacatc ttcgataaat agcacactac aactcgttaa
    15181 tcactttctg ttgttcacat ctcattgcaa actcaatatt gttgttacaa aatatccatg
    15241 aagcaagttt atattaaaca aacaactcgc ataaaacaat tgttatcctt aaattttaac
    15301 aaattcttaa taaatttatc tctattttaa ttacgaccaa at
//
LOCUS       DAKTIP010000049        15255 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAKTIP010000049 DAKTIP010000000
VERSION     DAKTIP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.049"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            191..406
                     /locus_tag="O0305_001136"
     CDS             191..406
                     /locus_tag="O0305_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274286.1"
                     /translation="MNKKSEGLDIIIPRIFRRDHASVESLTENERRLRKEILGSIKKG
                     DYSYLEINKVFYALDRELQYRANNNKL"
     gene            431..694
                     /locus_tag="O0305_001137"
     CDS             431..694
                     /locus_tag="O0305_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274287.1"
                     /translation="MPKIIVPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYRKDNLGVENLYIDYSPTGTLINISKLEEYLIRKHKKWY"
     gene            706..867
                     /locus_tag="O0305_001138"
                     /pseudo
     CDS             706..867
                     /locus_tag="O0305_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
     gene            960..1277
                     /locus_tag="O0305_001139"
     CDS             960..1277
                     /locus_tag="O0305_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274288.1"
                     /translation="MAENIKTEQHYYTKDFSGYRNEEDNFVANQELTVTITLNEYRKL
                     IEIKAVKDKEEDTYRGKYFAEERKNEKLEKENIKLKNKIYELQNEEDNEEDEENEEEV
                     NND"
     gene            1270..1572
                     /locus_tag="O0305_001140"
     CDS             1270..1572
                     /locus_tag="O0305_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2482 family protein"
                     /protein_id="HCY0274289.1"
                     /translation="MTKKYKDMTQEEVKDLLSEKSGELYELAKEIKGESKFDILLFSS
                     IGVIDGDYLAGSSSVIGHTFDLASLLDSTKSYKDIVNVLQMCKSQKFHGIDDNKEG"
     gene            1577..1837
                     /locus_tag="O0305_001141"
     CDS             1577..1837
                     /locus_tag="O0305_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCY0274290.1"
                     /translation="MYYKFGEIKNKIISFNGFEFKVSAMKKHDGISIQIKDMNNIPLK
                     SFHVVDLSELYFATDAMRDVINEWIEENTDEQDRLINLVMKW"
     gene            1851..2213
                     /locus_tag="O0305_001142"
     CDS             1851..2213
                     /locus_tag="O0305_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274291.1"
                     /translation="MAILEDIFEELKLLNNNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     EETAQLESIEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            2210..3376
                     /locus_tag="O0305_001143"
     CDS             2210..3376
                     /locus_tag="O0305_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCY0274292.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSTVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDNDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTIRMTIIQPRIDNFSTEELPISRLLQWGADFVKPLARL
                     AYNGGGEFKAGSHCRFCKINHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEQYALNQAKENDKNYPGWKLVEGRSRRMITDTKAMLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKITEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            3402..3959
                     /locus_tag="O0305_001144"
     CDS             3402..3959
                     /locus_tag="O0305_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCY0274293.1"
                     /translation="MKAKVLNKTKVITGKVRASYAHIFEPHSMQEGQESKYSISLIIP
                     KSDTSTIKAIEQAIEAAKEEGKVSKFGGKVPANLKLPLRDGDTEREDDVNYQDAYFIN
                     ASSKQAPGIIDQNKIRLTDSGTVVSGDYIRASINLFPFNTNGNKGIAVGLNNIQLVEK
                     GEPLGGASAAEDDFDELDTDDEDFL"
     gene            4027..5979
                     /locus_tag="O0305_001145"
     CDS             4027..5979
                     /locus_tag="O0305_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCY0274294.1"
                     /translation="MNIDIETYSSNDISKCGAYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDNEPFHADFETFKIALFDPAVKKYAFNANFERTCLAKHFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIAHKIKDFPVTAIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLNQAKHITGLENPNSPTQLLAWLKDDQGLDIPNLQKKTVQEYLKEATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGVRFYMKKGFLMIELPSGRALAYPKASVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            5992..6177
                     /locus_tag="O0305_001146"
     CDS             5992..6177
                     /locus_tag="O0305_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCY0274295.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            6177..6578
                     /locus_tag="O0305_001147"
     CDS             6177..6578
                     /locus_tag="O0305_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCY0274296.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKEREKIRKKAILNLYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            6578..6835
                     /locus_tag="O0305_001148"
     CDS             6578..6835
                     /locus_tag="O0305_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCY0274297.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            6838..7038
                     /locus_tag="O0305_001149"
     CDS             6838..7038
                     /locus_tag="O0305_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274298.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            7053..7295
                     /locus_tag="O0305_001150"
     CDS             7053..7295
                     /locus_tag="O0305_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274299.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHTHVVNGTYYFHGHIV
                     PGWQSVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            7310..7558
                     /locus_tag="O0305_001151"
     CDS             7310..7558
                     /locus_tag="O0305_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCY0274300.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEDQEND"
     gene            7551..8087
                     /locus_tag="O0305_001152"
     CDS             7551..8087
                     /locus_tag="O0305_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCY0274301.1"
                     /translation="MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWF
                     NTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEF
                     HNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKM
                     KRNHERQDGTADAGKGYV"
     gene            8124..8330
                     /locus_tag="O0305_001153"
     CDS             8124..8330
                     /locus_tag="O0305_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCY0274302.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAERKY
                     EAQVKRDAVIKVGQLFENIRECGK"
     gene            8327..8530
                     /locus_tag="O0305_001154"
     CDS             8327..8530
                     /locus_tag="O0305_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274303.1"
                     /translation="MTEVKIKTISDRVYYTTTDLASDDYINLVMNLVIEDFLPVKDVF
                     NNEVWVKRDEIESFTFIKEANDD"
     gene            8523..8759
                     /locus_tag="O0305_001155"
     CDS             8523..8759
                     /locus_tag="O0305_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274304.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKAPEEKAKIEDDFIKEIKDKDS
                     EFYSPTMANMNEYELRAMLRMMPSLIDTGDDNDD"
     gene            8752..9138
                     /locus_tag="O0305_001156"
     CDS             8752..9138
                     /locus_tag="O0305_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274305.1"
                     /translation="MIKKLKNMDGFDIFIVGILSLFGIFALLLVITLPIYTVASYQHK
                     ELHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKVE
                     VKTIGYRIHFLNLYPVLYEVKKVDKQ"
     gene            9135..9287
                     /locus_tag="O0305_001157"
     CDS             9135..9287
                     /locus_tag="O0305_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0274306.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYEKI
                     RAEVSW"
     gene            9355..9555
                     /locus_tag="O0305_001158"
     CDS             9355..9555
                     /locus_tag="O0305_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCY0274307.1"
                     /translation="MWIVISIVLAIFLLILLSSISHKMKTIEALEYMNSYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            9843..12290
                     /locus_tag="O0305_001159"
     CDS             9843..12290
                     /locus_tag="O0305_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCY0274308.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTYWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(12399..12497)
                     /locus_tag="O0305_001160"
     CDS             complement(12399..12497)
                     /locus_tag="O0305_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274309.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            12631..12921
                     /locus_tag="O0305_001161"
     CDS             12631..12921
                     /locus_tag="O0305_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCY0274310.1"
                     /translation="MKESTLEKYLVKEISKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            12902..14269
                     /locus_tag="O0305_001162"
     CDS             12902..14269
                     /locus_tag="O0305_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0274311.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKH
                     DKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            14282..14719
                     /locus_tag="O0305_001163"
     CDS             14282..14719
                     /locus_tag="O0305_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0274312.1"
                     /translation="MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQ
                     DSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK"
     gene            14876..15190
                     /locus_tag="O0305_001164"
     CDS             14876..15190
                     /locus_tag="O0305_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0274313.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNHLCQMC
                     LREDIVTDANIVHHIIYVDEDFNKALELDNLMSVCYGCHNKIHANGNDKSNLKKIRVL
                     KI"
BASE COUNT         5880 a         2056 c         3040 g         4279 t
ORIGIN      
        1 aatgatgtgt agagttttgc tttcagcatc ttttatatgt tcttgtactt taataacttc
       61 accattaatt aaatgtaaat ctaaagaagt aacgatttct ttatctaaat attgagtgag
      121 caatctctta tgtatactta tcacctcctt aggttgataa caacattata cacgaaaggt
      181 ggaacaacaa atgaacaaaa aatcagaagg gttagacata atcataccaa ggattttcag
      241 aagagatcat gcgtcagtag aatctttaac agaaaatgaa cgtcgactaa gaaaggaaat
      301 attaggaagt attaaaaaag gtgattacag ctacttagaa ataaacaaag ttttctatgc
      361 attagataga gaacttcaat acagagcgaa taataacaaa ctttaacatt tatcgaaagg
      421 agtgatagag atgccaaaaa tcatagtacc accaacacca gaaaacacat atagaggcga
      481 agaaaaattt gtgaaaaagt tatacgcaac acctacacaa atccatcaat tgtttggagt
      541 atgtagaagt acagtataca actggttgaa atattaccgc aaagataatt taggtgtaga
      601 aaatttatac attgattatt caccaacagg cactctgatt aatatttcta aattggaaga
      661 gtatttgatc agaaagcata aaaaatggta ttaggaggat attaaatgag cgacacatat
      721 aaaagctaat taatagcagt actgtgcttc acagtcttag cgattgtgct tatgccattg
      781 ctgtacttca ctacagcatg gtcaattgcg ggattcgcaa gtatcgcaac attcatatac
      841 tacaaagaat acttttatga agaataaaaa aactgctact tgcgtcaaca agtaacagtg
      901 acaaacattt atcaaaatat acaacttaat taaatcaaaa tatacggagg tagtcaacta
      961 tggctgaaaa tattaaaact gaacaacatt attacactaa agatttctca ggatacagaa
     1021 atgaagaaga taactttgta gcaaatcaag aattgacagt aacaatcaca ttgaacgagt
     1081 acagaaaact tattgaaata aaggctgtta aagataaaga agaagatact tacagaggta
     1141 agtattttgc ggaagaaaga aaaaacgaaa aattggaaaa agaaaatata aaactaaaaa
     1201 acaaaattta tgaattacaa aacgaagaag ataacgagga ggacgaagaa aacgaggagg
     1261 aagtcaacaa tgactaaaaa atataaagac atgactcagg aagaagtaaa agacttatta
     1321 tctgaaaaaa gcggagaatt gtatgaatta gcgaaagaaa ttaagggaga aagtaaattt
     1381 gatattttgc ttttctcatc aataggagtt atcgacggag attatttagc aggttcaagt
     1441 tctgtgattg gtcatacttt cgatcttgct tccttattgg atagcactaa gagttataaa
     1501 gacattgtca atgttctcca aatgtgtaaa tcacaaaaat ttcacggtat tgatgataac
     1561 aaggagggct aaaacaatgt attacaaatt tggtgagata aaaaataaaa ttatcagctt
     1621 taacgggttt gaatttaaag tgtctgcgat gaaaaaacat gacggtatca gtatacaaat
     1681 caaggatatg aataatattc cacttaaatc atttcatgtt gtagatttaa gcgaactata
     1741 ttttgcgacg gatgcaatgc gtgacgttat aaacgaatgg attgaagaga acacagatga
     1801 acaggacaga ctaattaact tagtcatgaa atggtaggag gatttaatca atggcaatat
     1861 tagaagatat ttttgaagaa ttaaaactat taaataacaa tttacgtgtg ttaaacactg
     1921 aactatcaac agtagattca tcaattgtac aagagaaagt taaagaagca ccaatgccaa
     1981 aagaagaaac agctcaactg gaatcaattg aagaagttaa ggaaacttct gctgatttaa
     2041 ctaaagatta tgttttatca gtaggaaaag agttccttaa aaaagcagat acttctgata
     2101 agaaagaatt tagaaataaa cttaacgaac ttggtgcgga taagctatct actatcaaag
     2161 aagagcatta tgaaaaaatt gttgatttta tgaatgcgag aataaatgca tgaagctaga
     2221 tcactcaaat agagctcatg caaagcttag tgcaagtgga gcaaaacaat ggctaaactg
     2281 cccaccgagt attaaggcaa gtgaaggtat tgcagataaa agtacagttt ttgccgaaga
     2341 aggtacattc gcccatgagt taagtgagtt atatttcagc cttaaatatg aaggcctaac
     2401 acagtttgag tttaataagg cttttcaaaa ttataagaga aatcaatatt acagcgaaga
     2461 attgcgtgaa tatgtggaag agtatgtagc taatgtagaa gaaaaatata acgaagcttt
     2521 gagtagggat aatgatgtaa tagctttatt tgaaacaaaa ttagatttag gtaaatacgt
     2581 ccctgaatct tttggaactg gtgatgtcat tatattttca ggtggtgtac ttgaaattat
     2641 tgaccttaaa tatggtaaag gcattgaagt ttcagctata gataatcctc aacttagatt
     2701 atatggcttg ggcgcatatg aactgcttag tttaatgtat gacattcata caattcgcat
     2761 gactatcata caacctcgaa tagataactt ttctactgaa gagttaccaa tatcaagatt
     2821 acttcaatgg ggagccgatt ttgttaaacc attagccaga cttgcttata acggtggagg
     2881 agagtttaaa gcaggtagtc attgtagatt ctgtaagata aatcattcat gtagaacacg
     2941 tgcagaatac atgcaaaatg tgcctcaaaa gccaccacat ttgttaagtg atgaagagat
     3001 tgcagaactt ttatataaac tgcctgatat caaaaaatgg gctgatgaag tagaacaata
     3061 tgcactaaat caagcgaaag aaaatgataa aaactatcct ggttggaagc ttgtagaagg
     3121 tcgttcacga agaatgataa ctgatacaaa agcaatgctt gaaaagttag ttgaagcggg
     3181 ttataaacct gaagatatta cagaaaccaa gttacttagc attacgaatt tagaaaaatt
     3241 aattggtaaa aaagcatttt ctaaaattac agagggtttt atagaaaagc cgcaaggtaa
     3301 attaacactt gctaccgagt cggataaacg accagctata aagcaatctg ctgaagatga
     3361 ttttgacaaa ctataaaaat ttaaaaggac ggtatataaa catgaaagca aaagtattaa
     3421 ataaaactaa agtgattaca ggaaaagtaa gagcatcata tgcacatatt tttgaacctc
     3481 acagtatgca agaagggcaa gaatcaaagt attcaatcag tttaatcatt cctaaatcag
     3541 atacaagtac gataaaagcc attgaacaag ctatagaagc tgctaaagaa gaaggaaaag
     3601 ttagtaagtt tggaggcaaa gttcctgcaa atctgaaact tccattacgt gatggagata
     3661 ctgaaagaga agatgatgtc aattatcaag acgcttattt tattaacgca tcaagcaaac
     3721 aagcacctgg tattattgac caaaacaaaa ttagattaac ggattctgga actgttgtaa
     3781 gtggtgatta tattagagct tcaattaatc tatttccttt caacacaaat ggtaataagg
     3841 gtatcgcagt tggattgaac aacattcaac ttgtagaaaa aggcgaacct cttggcggtg
     3901 caagtgcagc agaagatgat ttcgatgaat tagacactga tgatgaggat ttcttataag
     3961 tcaataggtg gggttttagc cccactttaa ttttaaagaa attgaggtgt caagaatttg
     4021 agatttatga atatagatat tgaaacatat agcagtaatg atatttcgaa atgtggtgcc
     4081 tataaataca cagaagctga agatttcgaa attttaatta tagcttattc aatagatggt
     4141 ggagcgatta gtgcgattga catgactaaa gtagataatg agcctttcca cgctgatttt
     4201 gagacgttta aaattgctct ttttgatcct gctgtaaaaa agtatgcatt caatgctaat
     4261 ttcgaaagaa cttgtcttgc taaacatttt aataaacaga tgccacctga agaatggatt
     4321 tgcacaatgg ttaattcaat gcgtattggc ttacctgctt cgcttgataa agttggagaa
     4381 gttttaagac tacaaaacca aaaagataaa gcaggtaaaa atttaattcg ttatttctct
     4441 ataccttgta aaccaacaaa agttaatgga ggaagaacaa gaaacttgcc tgaacatgat
     4501 cttgaaaaat ggcaacaatt tatagattac tgtattcgag atgtagaagt agaaatgacg
     4561 attgctcata aaattaaaga ctttccagta actgcaattg aacaagcata ttgggttttt
     4621 gaccaacata taaacgacag aggtattaag ctttctaaat cattgatgtt aggagctaat
     4681 gtgctcgata agcagagtaa agaagaattg cttaatcaag ctaaacatat aacaggttta
     4741 gaaaatccta atagtcctac acaattattg gcttggttaa aggatgacca aggattagat
     4801 atacctaatt tacaaaagaa aacggttcag gagtacttaa aagaagcaac aggaaaagct
     4861 aaaaaaatgc tagaaattag attgcaaatg tctaaaacca gtgtgaaaaa atacaacaaa
     4921 atgcatgaca tgatgtgcag tgatgaacgg gtaagaggtc tgtttcaatt ttacggtgcc
     4981 ggtactggaa gatgggcagg tagaggtgta caacttcaga atttaacaaa gcattatatt
     5041 tcagatactg aattagaaat agcaagagat cttattaaag aacaacgttt tgacgattta
     5101 gatttattac tcaatgttca tcctcaagac ttattaagtc aattagttag gacgacattt
     5161 actgctgaag aaggtaatga actagcagta agtgattttt ctgcaataga ggcaagagtc
     5221 atagcatggt atgcaaaaga acaatggcgt ttagatgtgt tcaacacaca cggaaagata
     5281 tatgaagcat cggcttctca aatgtttaat gtcccggtag aaagcataac taaaggcgac
     5341 cctctcagac aaaagggaaa agtgtccgaa ttagctttag gttatcaagg tggcgctgga
     5401 gctttaaaag cgatgggcgc attggaaatg ggcattgaag aaaatgaatt acaaggttta
     5461 gttgatagtt ggcgtaacgc aaatcctaac atagttaatt tttggaaggc ttgccaagag
     5521 gctgcaatta atactgtgaa atcccgaaag acgcatcata cgcatggagt tagattttat
     5581 atgaaaaaag gttttctaat gattgaactg cctagtggaa gagctttagc ttatccaaaa
     5641 gcttcagttg gtgaaaatag ttggggtagt caagttgttg aatttatggg cttagatctt
     5701 aaccgtaaat ggtcaaagtt aaaaacgtat ggtgggaagt tagtcgagaa tattgttcaa
     5761 gcaactgcaa gggatttact tgcgatttct atagctaggc ttgaagcatc aggttttaaa
     5821 atagttggac atgtccatga tgaagtaatt gtagaaatac ctagaggttc aaatggactt
     5881 aaggaaatcg aaactatcat gaataagcct gtcgattggg caaaaggatt gaatttgaat
     5941 agtgacggat ttacttctcc gttttatatg aaggattagg agtgtgattg aatgcaacat
     6001 caagcttata tcaatgcttc tgttgacatt agaattccta cagaagtcga aagtgttaat
     6061 tacaatcaga ttgataaaga aaaagagaat ttggcggact atttatttaa taatccaggt
     6121 gaactattaa aatataacgt tataaatatc aaggttttag atttagaggt ggaatgatgg
     6181 ctagaagaaa agttataaga gtgcgtatca aaggaaaact aatgacattg agagaagttt
     6241 cagaaaaata tcatatatct ccagaacttc ttagatacag atacaaacat aaaatgcgcg
     6301 gcgatgaatt attgtgtgga agaaaagact caaaatctaa agatgaagtt gaatatatga
     6361 agagtcaaat aaaagatgaa gaaaaagaga gagaaaaaat cagaaaaaaa gcgattttga
     6421 acctatacca acgaaatgtg agagcggaat atgaagaaga aagaaagaga agattgagac
     6481 catggcttta tgatggaacg cctcaaaaac attcacgtga tccgtactgg ttcgatgtca
     6541 cttataacca aatgttcaag aaatggagtg aagcataatg agcataatca gtaacagaaa
     6601 agtagatatg aatgaaacac aagacaatgt taaacaacct gcgcattaca catacggcga
     6661 cattgaaatt atagatttca tcgaacaagt tacggcacag tatccaccac aattagcatt
     6721 cgcaataggt aatgcaatca aatacttgtc tagagcaccg ttaaagaatg gtcatgagga
     6781 tttagcaaag gcgaagtttt acgtcgatag agtgtttgac ttgtgggagg ggtaacgatg
     6841 gcaacgcaaa aacaagttga ttacgtaatg tcattacagg agcaactgga attagaagac
     6901 tgcgaaaaat atacagacga acaagttaaa gcaatgagtc ataaagaagt tagcaatgtg
     6961 attgagaact ataagacaag cataaggaat gaagaactat attacgaatg catgtcgttt
     7021 ggactgccta attgttaaaa ggagtgacga ccatgacaga tagcgcacgt aaagaacgct
     7081 taaaccaatt tttcggctct aaaagatatc tgtatcagga taacgaacga gtggcacata
     7141 ctcatgtagt aaatggcacg tattactttc atgggcatat cgtgccaggt tggcaaagcg
     7201 ttaaaaagac atttgatact gctgaagagc tcgaaatata tataaagcaa catggtttgg
     7261 aatacgagga acagaagcaa ctaactttat tttagaggag atggaaatga tgaataaccg
     7321 cgaacaaatt gaacaatcaa ttatcagtgc tagtgcgtat aacggcaatg acacagaggg
     7381 attactaaaa gagattgaag acgtgtataa gaaagcgcaa gcgtttgatg aaatacttga
     7441 aggtttacct aatgctatgc aagatgcact caaagaagat attggtcttg atgaagcagt
     7501 agggattatg acggggcaag tggtctataa atatgaggag gatcaggaaa atgactaata
     7561 cattaacaat tgatcagtta caagagttat tacaaataca aaaggagttc gacgatagaa
     7621 taccaacgct gaacttacga gatagcaaaa tagcatatgt agttgaattc tttgaatggt
     7681 ttaatacatt ggaaacgttt aagaactgga agaagaaacc aggtaagccg ttagatgttc
     7741 agttagatga attagcggac atgttggcgt ttggattgag tattgcgaat caagtaggag
     7801 tgtcatcaga agagataaaa gaagcgattg aatcaagttt taaaaatagt gaatttcaca
     7861 acatgtttaa ttttaaagat aaagaatttg ctcaagacgc agttgttagc acaccacaga
     7921 taatattcaa agaattttat cctgaccaat tggcaattgt aatagcgata gacatagctt
     7981 tcaacttata ttctatcgac cagctcattg acgcatacaa aaagaaaatg aaaaggaatc
     8041 atgaaagaca agatggaaca gcagacgcag gaaaaggata cgtgtaaaga catcttagat
     8101 cgagtcaagg aggttttggg gaagtgacgc aatacttagt cacaacattc aaagattcaa
     8161 caggacgcaa gcatacacac ataactaaag ctaaaagcaa tcaaaggttt acagttgttg
     8221 aggcagagag taaagaagaa gctgagcgca aatacgaggc acaagttaaa agagatgcag
     8281 ttattaaagt gggtcagtta tttgaaaata taagggagtg tgggaaatga cggaggttaa
     8341 aattaaaact atttcagata gagtttatta cacaacaaca gatctagctt ctgacgatta
     8401 tattaatctt gttatgaatc tagtgattga ggattttctt ccggtcaaag atgtgttcaa
     8461 caatgaagta tgggttaaaa gagatgagat tgaatcattt acatttatta aggaggcaaa
     8521 cgatgattaa catacctaaa atgaaattcc cgaaaaagta cactgaaata atcaaaaaat
     8581 ataaaaataa agcacctgaa gaaaaggcta agattgaaga tgattttatt aaagaaatta
     8641 aagataaaga cagtgaattt tacagtccta cgatggctaa tatgaatgaa tatgaattaa
     8701 gggctatgtt aagaatgatg cctagtttaa ttgatactgg agatgacaat gatgattaaa
     8761 aaacttaaaa atatggatgg gttcgacatc tttattgttg gaatactgtc attattcggt
     8821 atattcgcat tgctacttgt tatcacattg cctatctata cagtggctag ttaccaacac
     8881 aaagaattac atcaaggaac tattacagat aaatataaca agagacaaga taaagaagac
     8941 aagttctata ttgtattaga caacaaacaa gtcattgaaa attccgactt attattcaaa
     9001 aagaaatttg atagcgcaga tatacaagct aggttaaaag taggcgataa ggtagaagtt
     9061 aaaacaatcg gttatagaat acacttttta aatttatatc cggtcttata cgaagtaaag
     9121 aaggtagata aacaatgatt aaacaaatac taagactatt attcttacta gcaatgtatg
     9181 agttaggtaa gtatgtaact gagcaagtgt atattatgat gacggctaat gatgatgtag
     9241 aggcgccgag tgattacgaa aaaatcagag ctgaagtttc atggtaatag ctattatcat
     9301 ttttgaatta attatattaa tgtgtttagc aatagcactg gaggtgttgt aaatatgtgg
     9361 attgtcattt caatcgtttt agctatattt ttattgatct tgttaagtag catttctcat
     9421 aagatgaaaa ccatagaagc attggagtat atgaattctt atcttttcaa gcagttagta
     9481 aaaaataatg gtgttgaagg tttagaagat tatgaaaatg aagttgaacg aattagaaaa
     9541 agattcaaaa gctaaagagg gggctaaagc cctctccagg attaaaagaa gcggatattt
     9601 tttatttcgt ttttgtaaat taaaactaat ttatgtgaaa ttgtctttga agtcttgata
     9661 attaagaagg ttgaattttc tgagattata ttagttacgg gaaaggcttt tcctgagtgc
     9721 aataaaatta aagttctcaa gttttcattt tcgtatttat cataaatgat ttcgcgtaaa
     9781 gttttcaaaa tttgtcacct ctttaatttt attaaattaa ttatacaaga aaggagccga
     9841 atatgttaga caaagtcact caaatagaaa caattaaata tgatcgtgat gtctcatatt
     9901 cttatgctgc tagtcgttta tccacatatt ggactaatca caatatggct tggtctgact
     9961 ttatgcagaa gctagcacaa acagttagaa ctaaagaaga tttaactgag tacaataaaa
    10021 tgtctaagtc tgaacaagca gatataaaag atgttggcgg atttgtcggt ggatatttaa
    10081 aagaaggcaa acgacgtgct ggtcaagtca tgaatcgttc aatgctaaca cttgatatcg
    10141 attatgctgc tcaagatatg actgacatat tatctatgtt ttatgatttt gcatattgtt
    10201 tatattcaac acataagcat agagagataa gtccaagact gcgtttagtg attcctttaa
    10261 aacgaaatgt aaatgcagat gagtatgaag ctattgggcg taaagtcgca gatatcgttg
    10321 gcatggatta cttcgatgat acaacttatc aaccacatag gttaatgtat tggccttcaa
    10381 ctagcaatga tgcagaattt ttctttacct atgaagattt acctttgtta gatccagata
    10441 aaatattaaa tgaatatgtt gattggactg acacattaga atggccaacg tcttcaaagg
    10501 aagagagtaa gactaaaaga ttagcagata agcaaggtga cccagaagaa aagccgggaa
    10561 ttgttggcgc attttgtaga gcctatacga tagaagaagc tatatcaact tttattcctg
    10621 acttatacga aaaacattct actaaccgtt atacctatca tgaaggttca actgcaggtg
    10681 gattggtgtt atacgaaaat aacaagtttg cctattctca tcataatacg gatcccgtta
    10741 gcggtatgct tgtgaacagt tttgatttag tacgcataca cttatatggt gctcaagatg
    10801 aagacgctaa aacagatact ccggttaatc gactacctag ttataaagca atgcagcaaa
    10861 gagcgcaaaa tgatgaagtt gttaaaaagc aattaattaa cgacaaaatg tctgatgcaa
    10921 tgcaggattt cgatgaaata gtaaatagcg atgatgcatg gtctgagacg ttagaaatta
    10981 cttcgaaagg tactttcaaa gctagtatcc caaatataga aattatattg cgtaatgatc
    11041 caaatttaaa aggaaaaata gcatttaatg aatttacaaa acaaattgaa tgcttaggga
    11101 aagtgccatg gaatactaat tttaagacac gtcaatggca agacggtgat gatagcagtt
    11161 taagaagtta tatcgaaaag atttatgaca tacaccattc aggtaaaaca aaagatgcca
    11221 ttataagcgt agcaatgcaa aatgcttatc atccagtaag ggattatcta aataaaatat
    11281 cgtgggatgg acataaacgt cttgaaaagt tatttatcaa atacttaggt gttgaagata
    11341 ctgaagtgaa tagaacaact accaaaaaag cattgactgc tggaattgct cgagtaatgg
    11401 agcctggatg taaatttgac tatatgctta cactttatgg tcctcaaggt gtaggtaaat
    11461 ctgctttgct aaaaaaatta ggtggtgcat ggttttctga cagtttagtt tctgttacag
    11521 gtaaagaagc ctatgaggcc ttacaaggcg tttggctaat ggaaatggca gaacttgcag
    11581 ctacaagaaa agctgaagtt gaagctatta agcatttcat atctaaacaa gttgaccggt
    11641 ttcgtgttgc ttatggacat tatattgaag attttccaag gcaatgtatt ttcattggta
    11701 caactaataa agttgatttc ttaagagatg aaactggtgg aagacgtttt tggccaatga
    11761 ctgtaaatcc agagagagtt gaagtgaact ggtctaaact aaccaaagaa gagatcgacc
    11821 aaatttgggc agaagctaaa tattattatg aacaaggaga agagttattc ctcaaccctg
    11881 aactagaaga agaaatgcgt tcaatacaaa gcaaacatac tgaggaatct ccatatacag
    11941 gcattattga tgaatatctt aacacaccaa ttcctagcaa ttgggatgac ttaactatct
    12001 ttgaacgaag acgattttat caaggtgatg ttgatatgtt accaacagga aatgtagatt
    12061 acgttgaaag aaataaggtc tgtgcgcttg aagtgtttgt tgaatgtttt ggtaaagata
    12121 agggagatag tagaggatct atggaaatta gaaagatttc aaacatctta agacaattag
    12181 acaattggtc tgtatatgat ggtaataaaa gtgggaaaat tcgatttgga aaagattatg
    12241 gtgtacagat agcttatgta agagatgaaa gtttagaaga tttaatataa taaatattga
    12301 ataaatatac attttagagt gttgtatcag atgttgcatc attttttgag tgatgcaaca
    12361 cgtgagtgta aaaagtaatc gtaggtgttg catcattttt agtgatgcaa cattgatgca
    12421 acaaatgata caacacctct ttcccttctc gctgtaaggt tcaaccctgt ttgtttccaa
    12481 tgttgcatca aattcactat aaagtttaaa aagtagtgtt agggagtaaa gaggtatagg
    12541 ggtaaccctc taacagctat ttttaaaagt ttggcaagaa ttgatgcaac atcggaacac
    12601 aaatataaat tttgtataca aggtgaatat atgaaagaat cgacattaga aaaatattta
    12661 gtgaaagaga tatcaaagct aaacggttta tgtttaaaat gggttgcacc tggaacaaga
    12721 ggtgtgccag atagaattat tattatgcca gaaggaaaaa catattttgt agaaatgaag
    12781 caagaaaaag gaaagttgca tcctttacaa aaatatgtgc atagacaatt tgaaaataga
    12841 gatcataaag tatatgtgtt atggaataaa gaacaagtaa atacttttat cagaatggta
    12901 ggtggaacat ttggcgattg acttcaaacc acatagctat caaaagtatg caatagataa
    12961 agtgatagat aatgagaaat acggtctgtt tttagatatg ggtctaggga aaacagtatc
    13021 aacacttaca gcatttagtg aattgcagtt gttagacact aaaaaaatgt tagttatagc
    13081 acctaaacaa gttgctaaag atacatgggt tgatgaagtt gataagtgga accatttaaa
    13141 tcatctgaaa gtgtctttag ttttaggaac acctaaagaa agaaatgatg cattaaacac
    13201 agaggctgat atctatgtaa ccaataaaga aaatactaaa tggttatgtg atcaatataa
    13261 aaaagaatgg ccatttgaca tggttgtgat tgatgaactg tctacattta aaagtcctaa
    13321 gagtcaaagg tttaaatcta ttaaaaagaa attaccactc attaatagat ttataggatt
    13381 aacaggaaca cctagtccaa atagtttaca ggatttatgg gctcaagttt atttgataga
    13441 cagaggtgaa agacttgagt cttcattcag tcgttatcga gaaaggtact ttaaaccaac
    13501 tcatcaagtt agcgaacata tttttaagtg ggagctaaga gacggatctg aagaaaagat
    13561 atataaacaa atagaagata tatgtttaag catgaaagcg aaagattatc tggatatgcc
    13621 tgacagagtt gatactaaac aaacagtagt cttatcagaa aaagaaagaa aagtatatga
    13681 agaattagaa aaaaactata ttttagaatc ggaagaagaa ggaacagttg tagctcaaaa
    13741 tggggcatca ttaagtcaga aactacttca actatctaac ggcgcagttt atacagatga
    13801 ggaagatgta agacttatac atgataagaa gttagataag ttagaggaaa ttatagagga
    13861 gtctcaaggc caaccaatac tattgtttta taacttcaaa cacgataaag aaagaatact
    13921 tcaaaggttt aaggaagcaa ccacattaga cgattcaaac tataaagaac gttggaacag
    13981 tggagacatt aagttgctta tagcacatcc agcaagtgca ggacatggat taaacttaca
    14041 acaaggtggg cacattattg tttggtttgg acttacatgg tccttggaat tataccaaca
    14101 agcaaatgct agattataca gacaaggaca aaatcatacg actattattc atcatatcat
    14161 gaccgataac acaatagatc aaagagtata taaagcttta caaaataaag aactaacgca
    14221 agaagaatta atgaaagcta ttaaagcaag aatagctaag cataagtaat ggaggtctaa
    14281 catggggaac acaatatatg atatcaagcc aggcacattt aaatatattg aatcagaaat
    14341 atataattta aatgagaaca agaaagaaat aaaaagattg aggttggaaa tacttaatcc
    14401 aacgaaggaa caagattcca atattgtata tggaccatta caaaaaggcg aaccagttag
    14461 aacaactgaa ctaatggcaa ctagattatt aactaataag atgttacgaa accttgaaga
    14521 aatggtcgaa gcagttgaga gtgaatactt aaagttgcct gaagatcata agaaagtaat
    14581 aaggctcaag tattggaata aagaaaagaa gttaaagatg gaacagatag gacatgaatg
    14641 ccatatgcat cgtaatactg ttactactat acgaaagaac tttgttaaag cggtagcgta
    14701 tcatgcaggt atcaaataac attgtgcaaa gattgtgcaa aaggcctaca aatctgtagt
    14761 aatatgatag tatcggaaag atgtataaag ttatctaaaa gttatacgac acaagtacac
    14821 gaggcacatc gctatgcggt gtgtcttttg ttatgcaatc aaagaggtgt aagagatgac
    14881 caagcacaat aacatttata agcatggtcg taagtcatat caatacgatt ggttctatca
    14941 ttcaaaagca tggaagaagt taagggagat agcattagat agagataatc atctttgtca
    15001 aatgtgttta cgcgaagata ttgtaacaga tgcaaacata gtgcatcaca ttatttatgt
    15061 tgatgaagat tttaacaaag ctttagaatt agataatcta atgtcagttt gttatggctg
    15121 tcataacaaa attcatgcaa atggtaatga caaaagtaat cttaagaaaa ttagagttct
    15181 aaaaatttaa ataaaaaaac atttaaataa aattttatag ccccctgccc attggcttaa
    15241 aatgtttttt cgccg
//
LOCUS       DAKTIP010000050        14574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAKTIP010000050 DAKTIP010000000
VERSION     DAKTIP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.050"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            292..1062
                     /locus_tag="O0305_001165"
     CDS             292..1062
                     /locus_tag="O0305_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCY0274314.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRVASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            1069..1851
                     /locus_tag="O0305_001166"
     CDS             1069..1851
                     /locus_tag="O0305_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCY0274315.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            2113..3399
                     /gene="rseP"
                     /locus_tag="O0305_001167"
     CDS             2113..3399
                     /gene="rseP"
                     /locus_tag="O0305_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCY0274316.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            3419..5122
                     /locus_tag="O0305_001168"
     CDS             3419..5122
                     /locus_tag="O0305_001168"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCY0274317.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
     gene            5386..9696
                     /locus_tag="O0305_001169"
     CDS             5386..9696
                     /locus_tag="O0305_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0274318.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            9986..10453
                     /gene="rimP"
                     /locus_tag="O0305_001170"
     CDS             9986..10453
                     /gene="rimP"
                     /locus_tag="O0305_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCY0274319.1"
                     /translation="MSKITEQVEVIVKPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            10474..11649
                     /gene="nusA"
                     /locus_tag="O0305_001171"
     CDS             10474..11649
                     /gene="nusA"
                     /locus_tag="O0305_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCY0274320.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDVALEDADTTESTEEVNDVSVETNVEKESE"
     gene            11670..11954
                     /locus_tag="O0305_001172"
     CDS             11670..11954
                     /locus_tag="O0305_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCY0274321.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            11951..12268
                     /locus_tag="O0305_001173"
     CDS             11951..12268
                     /locus_tag="O0305_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCY0274322.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            12273..14390
                     /gene="infB"
                     /locus_tag="O0305_001174"
     CDS             12273..14390
                     /gene="infB"
                     /locus_tag="O0305_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCY0274323.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIIQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
BASE COUNT         5257 a         1979 c         2887 g         4451 t
ORIGIN      
        1 agatgcatgt gataatttaa attcacaatg agcataaaaa tggtgtttaa acaagttaat
       61 taaacatata ctttataaat aataggcatt aggtatattg ctataataaa gttatgtaat
      121 ttttaacctc agtatgtatg tcacatttct ggtgtaaact gtaccgagtc agactttggt
      181 acagtttttt tatttgctta ttcaatgcat taaatgagta tgataaaatg ataatgattg
      241 tttagtaact tatactatat gacagagatg atcaggctcg gaggaaagac catgtttaaa
      301 aagctaataa ataaaaagaa cactataaat aattataatg aagaattaga ctcgtctaat
      361 atacctgaac atatcgctat tattatggat ggtaatgggc gatgggctaa gaagcgaaaa
      421 atgcctagaa ttaaaggtca ttacgaaggt atgcaaacaa taaaaaaaat tactagggta
      481 gctagtgata ttggtgttaa gtacttaact ttatacgcct tttccactga aaattggtca
      541 agacctgaaa gtgaagtaaa ttatattatg aatttgcctg tcaatttctt aaagacattc
      601 ttaccggaac taattgaaaa aaatgtcaaa gttgaaacaa ttggatttac tgataagttg
      661 ccaaaatcaa cgatagaagc aattaataat gctaaagaaa agacagctaa taataccggc
      721 ttaaaattaa tatttgcaat taattatggt ggcagagcag aacttgttca tagtattaaa
      781 aatatgtttg acgagcttca tcaacaaggt ttaaatagtg atatcataga tgaaacatat
      841 ataaacaatc atttaatgac aaaagactat cctgatccag agttgttaat tcgtacttca
      901 ggagaacaaa gaataagtaa tttcttgatt tggcaagttt cgtatagtga atttatcttt
      961 aatcaaaaat tatggcctga ctttgacgaa gatgaattaa ttaaatgtat aaaaatttat
     1021 cagtcacgtc aaagacgctt tggcggattg agtgaggagt agtatagtat gaaagttaga
     1081 acgctgacag ctattattgc cttaatcgta ttcttgccta tcttgttaaa aggcggcctt
     1141 gtgttaatga tatttgctaa tatattagca ttgattgcat taaaagaatt gttgaatatg
     1201 aatatgatta aatttgtttc agttcctggt ttaattagtg cagttggtct tatcatcatt
     1261 atgttgccac aacatgcagg gccatgggta caagtaattc aattaaaaag tttaattgca
     1321 atgagcttta ttgtattaag ttatactgtc ttatctaaaa acagatttag ttttatggat
     1381 gctgcatttt gcttaatgtc tgtggcttat gtaggcattg gttttatgtt cttttatgaa
     1441 acgagatcag aaggattaca ttacatatta tatgcctttt taattgtttg gcttacagat
     1501 acaggggctt acttgtttgg taaaatgatg ggtaaacata agctttggcc agtaataagt
     1561 ccgaataaaa caatcgaagg attcataggt ggcttgttct gtagtttgat agtaccactt
     1621 gcaatgttat attttgtaga tttcaatatg aatgtatgga tattacttgg agtgacattg
     1681 attttaagtt tatttggtca attaggtgat ttagtggaat caggatttaa gcgtcatttc
     1741 ggcgttaaag actcaggtcg aatactacct ggacacggtg gtattttaga ccgatttgac
     1801 agctttatgt ttgtgttacc attattaaat attttattaa tacaatctta atgctgagaa
     1861 caaatcaata aacgtaaaga ggagttgctg agataattta atgaatctca gaactccttt
     1921 tgaaaattat acgcaatatt aactttgaaa attatacgca atattaactt tgaaaattat
     1981 acgcaatatt aactttgaaa attagacgtt atattttgtg atttgtcagt atcatattat
     2041 aatgacttat gttacgtata cagcaatcat ttttaaaata aaagaaattt ataaacaatc
     2101 gaggtgtagc gagtgagcta tttagttaca ataattgcat ttattattgt ttttggtgta
     2161 ctagtaactg ttcatgaata tggccatatg ttttttgcga aaagagcagg cattatgtgt
     2221 ccagaatttg cgatcggtat ggggccaaaa atttttagtt ttagaaaaaa tgaaacactt
     2281 tacactatta ggttattgcc tgttggtgga tatgttcgta tggcaggaga tggcttagaa
     2341 gagccaccag tcgagcccgg tatgaacgtt aaaattaaac ttaatgaaga aaatgaaata
     2401 acacatatca tattagatga tcatcataag tttcaacaaa ttgaagcgat cgaagttaaa
     2461 aaatgtgatt ttaaggatga cttattcata gaaggtatca ctgcttatga taatgaaaga
     2521 catcatttta aaattgctag aaagtctttc tttgttgaaa atggtagctt agttcaaatt
     2581 gctccgagag acagacaatt tgcacataaa aagccatggc cgaaattttt aacattattt
     2641 gcgggaccgt tatttaactt tatattagct ttagtcctat ttattggtct tgcatattat
     2701 caaggcacgc ctacgtctac tgtagaacaa gtcgcagata agtatccagc tcaacaagca
     2761 ggattacaaa aaggtgataa gatcgtccaa attggcaaat ataaaatatc tgaatttgat
     2821 gatgttgata aggcgttaga taaagttaaa gataataaga cgactgttaa atttgaacgt
     2881 gatggtaaaa caaagtcagt tgaattaaca cctaaaaaga ctgaaaaaaa actgactaaa
     2941 gtaagttcag agacgaagta tgttctcgga ttccaaccag cgagtgaaca tacacttttt
     3001 aaaccaattg tattcggatt taaaagcttt ttaatcggta gtacttatat ttttacagct
     3061 gtagtaggta tgttggctag tatatttacg ggcggattct catttgatat gttaaatggt
     3121 ccggttggta tttatcataa cgtcgactca gttgttaaag cgggtatcat tagcttaatt
     3181 ggttacactg cgttattaag tgtaaactta ggtattatga atttaattcc tattcctgca
     3241 ctagacggtg gtcgtatttt atttgttata tatgaagcga ttttcagaaa accagttaat
     3301 aaaaaagcgg aaacaacgat tattgctatt ggtgccattt tcatggtcgt tataatgata
     3361 ttagtaacgt ggaatgatat tcgacgatat ttcttataat ttaggaggat aaataattat
     3421 gaagcaatcc aaagttttta taccaacgat gcgtgacgtg ccatcagaag cagaagcaca
     3481 aagtcatcgt ttattattga aatcgggttt gataaaacaa agtacaagtg ggatttatag
     3541 ttatttaccg ctagcaacac gtgtgttaaa taatattact gcaattgtgc gacaagaaat
     3601 ggaacgtatc gattctgttg aaattttaat gccagcgtta caacaagctg aattatggga
     3661 agaatcagga cgttggggtg catatggccc agaattaatg cgtttacaag atagacatgg
     3721 aagacaattt gcattaggtc caacacatga agaattagtt acatcaatag taagaaatga
     3781 attgaaatca tacaaacaat taccgatgac attattccaa attcaatcta aattccgtga
     3841 tgaaaagaga ccacgttttg gtttacttcg tgggcgtgaa tttattatga aagatgcata
     3901 ttcattccat gctgacgagg catcattaga tcaaacgtat caagatatgt atcaagcgta
     3961 tagccgtatt tttgagagag ttggcattaa cgcaagacca gtagttgcag attcaggtgc
     4021 tataggcggt agccatacac atgaatttat ggcattaagt gctatcggtg aggatacaat
     4081 cgtttacagt aaagaaagtg attatgctgc taacatcgaa aaagcagaag tcgtttacga
     4141 accaaatcat aagcatacta ctgtgcaacc tttagaaaaa attgaaacac caaatgttaa
     4201 gactgcgcaa gaattggcag acttcttagg tagaccagta gatgaaatcg ttaaaacgat
     4261 gattttcaaa gttgatggcg aatatattat ggttttagtg cgtggccatc atgaaattaa
     4321 tgacattaaa ttaaaatctt atttcggcac agataatatt gaattagcaa cacaagacga
     4381 aattgttaat ttagttggtg caaatcctgg ttcactaggt cctgtaattg ataaagaaat
     4441 caaaatttat gcagataatt ttgtgcaaga tttaaataat ttagttgtcg gtgctaacga
     4501 agatggttat cacttaatta atgtaaatgt aggtagagac ttcaacgttg atgaatatgg
     4561 cgatttccgt tttattttag aaggcgaaaa gttaagtgat ggttcaggcg ttgcacattt
     4621 tgctgaaggt attgaagttg gtcaagtatt caaattgggt actaagtatt cagaatcaat
     4681 gaatgctaca ttcttagata accaaggaaa agctcaatct ttaattatgg gttgttacgg
     4741 aattggaatt tctagaacgc taagtgcgat tgttgaacaa aatcacgatg ataatggaat
     4801 tgtttggcct aaatcagtta ctccgtttga tttacattta atttctatta atcctaagaa
     4861 agatgatcaa cgagaactag cagatgcact atatgctgaa tttaatacta aatttgatgt
     4921 gttgtacgat gatcgtcagg aacgtgcagg tgttaaattt aatgatgccg atttaattgg
     4981 tttaccactg cgaattgttg ttggtaaacg tgcatcggaa ggtattgtag aagttaaaga
     5041 acgtttaaca ggtgatagcg aagaagttca cattgatgac ttaatgactg tcattacaaa
     5101 taaatatgat aacttaaaat aattaagatc gaatgaatta taagagtagg aaaaagctga
     5161 aagaaatctg atgcttatgt cctgctctta ttatttttga tataatgatt attcgatgaa
     5221 aaatgactga agacatagta taattaaaga taaatttgtt ttaacaatat aatgattagc
     5281 caaatataaa gcatttaatt ttctatcatt actatgctca cataatctaa atattgttcg
     5341 aacacgtaaa agtaatttct atttaaggtg gtaattgtct tggcaatgac agagcaacaa
     5401 aaatttaaag tgcttgctga tcaaattaaa atttcaaatc aattagatgc tgaaatttta
     5461 aattcaggtg aactgacacg tatagatgtt tctaacaaaa acagaacatg ggaatttcat
     5521 attacattac cacaattctt agctcatgaa gattatttat tatttataaa tgcaatagag
     5581 caagagttta aagatatcgc caacgttaca tgtcgtttta cggtaacaaa tggcacgaat
     5641 caagatgaac atgcaattaa atactttggg cactgtattg accaaacagc tttatctcca
     5701 aaagttaaag gtcaattgaa acagaaaaag cttattatgt ctggaaaagt attaaaagta
     5761 atggtatcaa atgacattga acgtaatcat tttgataagg catgtaatgg aagtcttatc
     5821 aaagcgttta gaaattgtgg ttttgatatc gataaaatca tattcgaaac aaatgataat
     5881 gatcaagaac aaaacttagc ttctttagaa gcacatattc aagaagaaga cgaacaaagt
     5941 gcacgattgg caacagagaa acttgaaaaa atgaaagctg aaaaagcgaa acaacaagat
     6001 aacaacgaaa gtgctgtcga taagtgtcaa attggtaagc cgattcaaat tgaaaatatt
     6061 aaaccaattg aatctattat tgaggaagag tttaaagttg caatagaggg tgtcattttt
     6121 gatataaact taaaagaact taaaagtggt cgccatatcg tagaaattaa agtgactgac
     6181 tatacggact ctttagtttt aaaaatgttt actcgtaaaa acaaagatga tttagaacat
     6241 tttaaagcgc taagtgttgg taaatgggtt agggctcaag gtcgtattga agaagataca
     6301 tttattagag atttagttat gatgatgtct gatattgaag agattaaaaa agcgacaaaa
     6361 aaagataagg ctgaagaaaa gcgtgtagaa ttccacttgc atactgcaat gagccaaatg
     6421 gatggtatac ccaatattgg tgcgtatgtt aaacaggcag cagactgggg acatccagcc
     6481 attgcggtta cagaccataa tgttgtgcaa gcatttccag atgctcacgc agcagcggaa
     6541 aaacatggca ttaaaatgat atacggtatg gaaggtatgt tagttgatga tggtgttccg
     6601 attgcataca aaccacaaga tgtcgtatta aaagatgcta cttatgttgt gttcgacgtt
     6661 gagacaactg gtttatcaaa tcagtatgat aaaatcatcg agcttgcagc tgtgaaagtt
     6721 cataacggtg aaatcatcga taagtttgaa aggtttagta atccgcatga acgattatcg
     6781 gaaacgatta tcaatttgac gcatattact gatgatatgt tagtagatgc ccctgagatt
     6841 gaagaagtac ttacagagtt taaagaatgg gttggcgatg cgatattcgt agcgcataat
     6901 gcttcgtttg atatgggctt catcgatacg ggatatgaac gtcttgggtt tggaccatca
     6961 acgaatggtg ttatcgatac tttagaatta tctcgtacga ttaatactga atatggtaaa
     7021 catggtttga atttcttggc taaaaaatat ggcgtagaat taacgcaaca tcaccgtgcc
     7081 atttatgata cagaagcaac agcttacatt ttcataaaaa tggttcaaca aatgaaagaa
     7141 ttaggcgtat taaatcataa cgaaatcaac aaaaaactca gtaatgaaga tgcatataaa
     7201 cgtgcaagac ctagtcatgt cacattaatt gtacaaaacc aacaaggtct taaaaatcta
     7261 tttaaaattg taagtgcatc attggtgaag tatttctacc gtacacctcg aattccacgt
     7321 tcattgttag atgaatatcg tgagggatta ttggtaggta cagcgtgtga tgaaggtgaa
     7381 ttatttacgg cagttatgca gaaggaccag agtcaagttg aaaaaattgc caaatattat
     7441 gattttattg aaattcaacc accggcactt tatcaagatt taattgatag agagcttatt
     7501 agagatactg aaacattaca tgaaatttat caacgtttaa tacatgcagg tgacacagcg
     7561 ggtatacctg ttattgcgac aggaaatgca cactatttgt ttgaacatga tggtatcgca
     7621 cgtaaaattt taatagcatc acaacccggc aatccactta atcgctcaac tttaccggaa
     7681 gcacatttta gaactacaga tgaaatgtta aacgagtttc attttttagg tgaagaaaaa
     7741 gcgcatgaaa ttgttgtgaa aaatacaaac gaattagcag atcgaattga acgtgttgtt
     7801 cctattaaag atgaattata cacaccgcgt atggaaggtg ctaacgaaga aattagagaa
     7861 ctaagttatg caaatgcgcg taaactgtat ggtgaagacc tgcctcaaat cgtaattgat
     7921 cgattagaaa aagaattaaa aagtattatc ggtaatggat ttgcggtaat ttacttaatt
     7981 tcgcaacgtt tagttaaaaa atcattagat gatggatact tagttggttc ccgtggttca
     8041 gtaggttcta gttttgtagc gacaatgact gagattactg aagtaaaccc gttaccgcca
     8101 cactatattt gtccgaactg taaaacgagt gaatttttca atgatggttc agtaggatca
     8161 ggatttgatt tacctgataa gacgtgtgaa acttgtggag cgccacttat taaagaagga
     8221 caagatattc cgtttgaaac atttttagga tttaagggag ataaagttcc tgatatcgac
     8281 ttaaacttta gtggtgaata tcaaccgaat gcccataact acacaaaagt attatttggt
     8341 gaggataaag tattccgtgc aggtacaatt ggtactgttg ctgaaaagac tgcttttggt
     8401 tatgttaaag gttatttgaa tgatcaaggt atccacaaaa gaggtgctga aatagatcga
     8461 ctcgttaaag gatgtacagg tgttaaacgt acaactggac agcatccagg gggtattatt
     8521 gtagtacctg attacatgga tatttatgat tttacgccga tacaatatcc tgccgatgat
     8581 caaaattcag catggatgac gacacatttt gatttccatt ctattcatga taatgtatta
     8641 aaacttgata tacttggaca cgatgatcca acaatgattc gtatgcttca agatttatca
     8701 ggaattgatc caaaaacaat acctgtagat gataaagaag ttatgcagat atttagtaca
     8761 cctgaaagtt tgggtgttac tgaagatgaa attttatgta aaacaggtac atttggggta
     8821 ccagaattcg gtacaggatt cgtgcgtcaa atgttagaag atacaaagcc aacaacattt
     8881 tctgaattag ttcaaatctc aggattatct catggtacag atgtgtggtt aggcaatgct
     8941 caagaattaa ttaaaaccgg tatatgtgat ttatcaagtg taattggttg tcgtgatgat
     9001 atcatggttt atttaatgta tgctggttta gaaccatcaa tggcttttaa aataatggag
     9061 tcagtacgta aaggtaaagg tttaactgaa gaaatgattg aaacgatgaa agaaaatgaa
     9121 gtgccagatt ggtatttaga ttcatgtctt aaaattaagt acatgttccc taaagcccat
     9181 gcagcagcat acgttttaat ggcagtacgt atcgcatatt tcaaagtaca tcatccactt
     9241 tattactatg catcttactt tacaattcgt gcgtcagact ttgatttaat cacgatgatt
     9301 aaagataaaa caagcattcg aaatactgta aaagacatgt attctcgcta tatggatcta
     9361 ggtaaaaaag aaaaagacgt attaacagtc ttggaaatta tgaatgaaat ggcgcatcga
     9421 ggttatcgaa tgcaaccgat tagtttagaa aagagtcagg cgttcgaatt tatcattgaa
     9481 ggcgatacac ttattccgcc gttcatatca gtgcctgggc ttggcgaaaa cgttgcgaaa
     9541 cgaattgttg aagctcgtga cgatggccca tttttatcaa aagaagattt aaacaaaaaa
     9601 gctggattat ctcagaaaat tattgagtat ttagatgagt taggctcatt accgaattta
     9661 ccagataaag ctcaactttc gatatttgat atgtaaaatg aaataatcaa ggtatttatt
     9721 taatgcgtat ggcgtagtca aagaaataca aaattgttgc tggacacaaa attatgcccg
     9781 tatttctttt caatgtctta cgagtctatt caaatgtaat ggtgaaataa aggaacaaac
     9841 ttttacaaga atctctgatt aatagtgaag tcatttgttt caagcataaa cttatgctat
     9901 aattaagttg cttaaaaatt agtgaactca ggcagaagag tgggagattc ccgctctttt
     9961 ctatttgcca aaaagggagg cctgtatgag taaaattaca gaacaagtag aagtgattgt
    10021 taaaccaatt atggaagact tgaattttga acttgtagac gttgaatatg tcaaagaggg
    10081 tagagatcat tttcttagaa tctctattga taaagaaggt ggcgtagatt taaatgattg
    10141 tacgctagct tctgaaaaaa taagtgaagc tatggatgca aatgatccta ttcctgaaat
    10201 gtattattta gacgtagcgt cacctggtgc agaacgtcca attaaaaaag aacaagattt
    10261 ccaaaatgca ataactaaac ctgtatttgt ttctttatat gtaccaattg aaggtgaaaa
    10321 ggaatggtta ggcattttac aagaagtcaa taatgaaaca attgtagtac aagttaaaat
    10381 caaagcaaga acgaaagata tagagatacc gagagacaaa atagcaaaag cacgtcacgc
    10441 agttatgatt taacgtgatg aggaggaaaa aacgtgtcaa gtaatgaatt attattagct
    10501 actgagtatt tagaaaaaga aaagaagatt cctagagcag tattaattga tgctattgaa
    10561 gcagctttaa ttactgcata caaaaagaat tatgatagtg caagaaatgt ccgtgtggaa
    10621 ttaaatatgg atcaaggtac tttcaaagtt atcgctcgta aagatgttgt tgaagaagta
    10681 tttgacgaca gagatgaagt ggatttaagt acagcgcttg ttaaaaaccc tgcatatgaa
    10741 attggtgata tatacgaaga agatgtaaca cctaaagatt ttggtcgtgt aggtgctcaa
    10801 gcagcgaaac aagcagtaat gcaacgtctt cgtgatgctg aacgtgaaat tttatttgaa
    10861 gaatttatag acaaagaaga agacatactt actggaatta ttgaccgtgt tgaccatcgt
    10921 tatgtatatg tgaatttagg tcgtatcgaa gctgttttat ctgaagcaga aagaagtcct
    10981 aacgaaaaat atattcctaa cgaacgtatc aaagtatatg ttaacaaagt ggaacaaacg
    11041 acaaaaggtc ctcaaatcta tgtttctcgt agccatccag gtttattaaa acgtttattt
    11101 gaacaagaag ttccagaaat ttacgatggt actgtaattg ttaaatcagt agcacgtgaa
    11161 gctggcgatc gctctaaaat tagtgtcttc tctgaaaaca atgatataga tgctgttggt
    11221 gcatgtgttg gtgctaaagg cgcacgtgtt gaagctgttg ttgaagagct aggtggtgaa
    11281 aaaatcgaca tcgttcaatg gaatgaagat ccaaaagtat ttgtaaaaaa tgctttaagc
    11341 ccttctcaag ttttagaagt tattgttgat gaaacaaatc aatctacagt agttgttgtt
    11401 cctgattatc aattgtcatt agcgattggt aaaagaggac aaaacgcacg tctagctgct
    11461 aaattaaccg gctggaaaat tgatattaaa tcagaaacag atgcgcgtga agcgggtatc
    11521 tatccagtag ttgaagctga aaaagtaact gaagaagatg ttgctttaga agatgctgac
    11581 acaacagaat caaccgaaga ggtaaatgat gtttcagttg aaacaaatgt agagaaagaa
    11641 tctgaataat aggttggagt gaagtatcta tgaaaaagaa aaaaattccg atgcgaaaat
    11701 gtattctttc aaatgaaatg catcccaaaa aagatatgat tcgtgttgtt gttaataaag
    11761 aaggcgaaat ctttgcggat gttactggaa agaaacaagg ccgtggcgca tatgtttcta
    11821 aagatgttgc tatggttgaa aaagcacaac aaaaagaaat tttagaaaaa tattttaaag
    11881 catctaaaga gcaattggat cctgtttaca aagaaattat tagattaatt tatagagaag
    11941 agatcccaaa atgagtatag atcaaatatt aaacttttta ggattagcaa tgagagctgg
    12001 taaagtaaaa acaggtgaat cagtcattgt taatgagatt aaaaaaggaa atttgaagct
    12061 cgttattgtt gcaaatgatg cgtctgataa tacagctaaa ttaattacag ataaatgtaa
    12121 gagttacaaa gttccattca gaaagtttgg aaatcgaaat gaattgggaa tagcacttgg
    12181 aaaaggtgag cgtgttaatg tagggattac tgacccaggc tttgctaaaa agttgctatc
    12241 aatgatagat gaatatcata aggagtgatt atatgagtaa acaaagaatt tacgaatatg
    12301 cgaaagaatt aaatctaaag agtaaagaga ttatagatga gttaaaaagc atgaatattg
    12361 aggtttcaaa tcatatgcaa gctttggaag atgaccaaat taaagcatta gataaaaagt
    12421 tcaaaaaaga acaaaagaac gacaataaac aaagcactca aaataatcac caaaaatcaa
    12481 acaatcaaaa ccaaaataaa gggcaacaaa aagataacaa aaagaatcaa caacaaaata
    12541 ataaaggcaa caaaggcaat aaaaagaata atagaaataa taagaaaaat aacaagaata
    12601 ataaaccaca aaatcaacca gctgctccaa aagaaatacc atcaaaagtg acatatcaag
    12661 aaggtattac agtaggcgaa tttgcggata aattaaatgt tgaatcatca gaaattatca
    12721 aaaaattatt cttacttggt attgttgcta atatcaatca atcattaaat caagaaacaa
    12781 tcgaattaat tgccgatgat tatggcgttg aggttgaaga agaagttgtg attaatgaag
    12841 aagacttatc aatctatttc gaagacgaaa aagatgatcc agaggcaatt gagagaccag
    12901 cagttgtaac aattatggga catgttgacc atggtaaaac gactttatta gattcaattc
    12961 gtcatacaaa agttacagca ggtgaagcag gcggaatcac tcaacatatt ggtgcatatc
    13021 aaattgaaaa cgatggcaaa aaaatcactt tcttagatac accgggacat gctgcattta
    13081 caacgatgcg tgcgcgtggt gcacaagtaa cagatattac tattttagta gtagcagctg
    13141 acgatggtgt tatgccacaa acaattgaag caattaacca tgctaaagaa gcagaagtac
    13201 caattattgt tgcagtaaat aaaattgata aaccaacttc aaatcctgat cgagttatgc
    13261 aagaattaac tgaatatggt ttaattcctg aagattgggg cggcgaaaca attttcgttc
    13321 cactttctgc attaagtggt gatggtatcg acgatttatt agaaatgata ggattagttg
    13381 cagaagttca agaacttaaa gcaaatccta aaaaccgtgc tgttggtaca gttatcgaag
    13441 ctgaattaga taaatcacgt ggtccttctg catcattatt agtacaaaac ggtacattaa
    13501 atgttggtga tgcgattgta gttggtaata cttacggccg tatccgtgca atggttaatg
    13561 acttaggtca aagaatcaaa acggctggtc catcaacgcc tgttgaaatt acaggtatta
    13621 atgatgtgcc acaagctggg gatcgctttg ttgtatttag tgatgaaaaa caagctcgtc
    13681 gtattggtga atcaagacac gaagctagca ttatacaaca acgtcaagaa agtaaaaatg
    13741 tttcattaga taacctgttt gaacaaatga aacaaggtga aatgaaagat ttaaacgtta
    13801 ttattaaagg tgatgttcaa ggttctgttg aagctttagc tgcatcatta atgaaaattg
    13861 atgttgaagg cgtaaatgtt cgtatcattc atacagcggt tggtgcaatt aatgagtcag
    13921 acgtgacact tgctaatgcc tcaaatggta ttatcattgg tttcaatgtt cgtccagaca
    13981 gtggtgcaaa acgtgctgca gaagctgaaa atgttgatat gcgtttacac agagttattt
    14041 ataatgttat cgaagaaatt gaatcagcga tgaaaggttt acttgatcca gaatttgaag
    14101 aacaagttat cggacaagct gaagttcgtc aaacattcaa agtttctaaa gttggtacta
    14161 ttgctggatg ttatgttact gaaggtaaaa ttacgcgaaa tgctggtgta cgtattattc
    14221 gtgatggtat tgttcaatat gaaggcgaat tagatacact taaacgtttc aaagatgatg
    14281 ctaaggaagt tgcaaaaggt tatgaatgtg gtattacaat tgaaaactac aatgacctta
    14341 aagaaggcga tgttatcgaa gcatttgaaa tggttgaaat taagcgttaa ttaaataaat
    14401 tacaagctaa aagtatagtt aagattgata tgctccctat aaatattgca ctttttaagt
    14461 gtctacttta tagggagcat atttgatact agcttttggt tttttattag aatagattac
    14521 ctattaaaag ttacgttata tggacatgat tttgtataaa attttgtggt ggcc
//
LOCUS       DAKTIP010000051        14511 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAKTIP010000051 DAKTIP010000000
VERSION     DAKTIP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14511
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.051"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..886
                     /gene="lukS-PV"
                     /locus_tag="O0305_001175"
     CDS             <1..886
                     /gene="lukS-PV"
                     /locus_tag="O0305_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     S"
                     /protein_id="HCY0274324.1"
                     /translation="IATSFHESKADNNIENIGDGAEVVKRTEDTSSDKWGVTQNIQFD
                     FVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLI
                     NYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVERQN
                     SKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYFVPDNELPPLVHSGFNP
                     SFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLEGSRIHNAFVNRNYT
                     VKYEVNWKTHEIKVKGHN"
     gene            882..1865
                     /gene="lukF-PV"
                     /locus_tag="O0305_001176"
     CDS             882..1865
                     /gene="lukF-PV"
                     /locus_tag="O0305_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panton-Valentine bi-component leukocidin subunit
                     F"
                     /protein_id="HCY0274325.1"
                     /translation="MDMKKIVKSSVVTSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDSDKLKISQILTFNFIKDKSYDKDTLILKAAGNIYSGYTKPNPKDTISSQF
                     YWGSKYNISINSDSNDSVNVVDYAPKNQNEEFQVQQTVGYSYGGDINISNGLSGGGNG
                     SKSFSETINYKQESYRTSLDKRTNFKKIGWDVEAHKIMNNGWGPYGRDSYHSTYGNEM
                     FLGSRQSNLNAGQNFLEYHKMPVLSRGNFNPEFIGVLSRKQNAAKKSKITVTYQREMD
                     RYTNFWNQLHWIGNNYKDENRATHTSIYEVDWENHTVKLIDTQSKEKNPMS"
     gene            2114..2299
                     /locus_tag="O0305_001177"
     CDS             2114..2299
                     /locus_tag="O0305_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274326.1"
                     /translation="MFILNTQAHARLDQMAQQFEVVCNGLNENEGQAIQTMDQSASLI
                     RSNLIQVKEQLEKLAVY"
     gene            2390..3307
                     /locus_tag="O0305_001178"
     CDS             2390..3307
                     /locus_tag="O0305_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0274327.1"
                     /translation="MFKRTKLILIATLLLSGCSTTNNESNKETKSVPEEMEASKYVGQ
                     GFQPPAEKDVVEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGKGVEVFVHCDDH
                     DIVFNASIPFDKSIIESDSSLRSEDKGDDMSTLVGTVLSGFEYRTQKEKYDNLYKFFK
                     DNEEKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIHKNDKEFKE
                     GMEQARKEVNYAANTDTVTTLFSTKENFTKDNTVDDVIELSDKLYNFKNKPEKSTITI
                     QIGKPTINTKKAFYDDNDPIEYGVYRKDE"
     gene            3364..4302
                     /locus_tag="O0305_001179"
     CDS             3364..4302
                     /locus_tag="O0305_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0274328.1"
                     /translation="MFKKAKLILIATLLLSGCSAMNNESKKDTNTETNTETKSVPEEM
                     EASKYVGQGFQPPAEKDAIEFVKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVE
                     VYVHCEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKY
                     DNLYKFLKENEKQYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIH
                     KNDKEFKEGMQRARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDM
                     PIKNTITTQLGNKLIGTKKARFDDKKVVSFGAFEDE"
     gene            4295..4501
                     /locus_tag="O0305_001180"
     CDS             4295..4501
                     /locus_tag="O0305_001180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274329.1"
                     /translation="MNKINDRDLTELSSYRVYQDINKDNDFTVNEKRFKQADVFEDLY
                     REKLKDTNKLREYNYLQNETFKSA"
     gene            4516..5430
                     /locus_tag="O0305_001181"
     CDS             4516..5430
                     /locus_tag="O0305_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCY0274330.1"
                     /translation="MLKKAKFILMATILLSGCSTTNNESNKETKSVPEEMDASKYVGQ
                     GFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVHCDDH
                     DIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGAVLSGFEYRAQKEKYDKLYKFFK
                     DNEEKYQYTGFTKEAINKTQNSGYENEYFYISAIPYNLAEYRDYFEPLLNKSDSEFSK
                     ELSNVKKQLKDKSKVSVTTTLFSKKKNYTKKSNSENVIKMAEEIKKDKEIPNGIELSI
                     KFSDNKINTVKPNFNGESTSEYGVFDQE"
     gene            5629..5748
                     /locus_tag="O0305_001182"
     CDS             5629..5748
                     /locus_tag="O0305_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274331.1"
                     /translation="MTIFTQLSDRIKKAISKINQANNIPNNARKSPMYHSPIT"
     regulatory      5831..5965
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6027..6572
                     /locus_tag="O0305_001183"
     CDS             6027..6572
                     /locus_tag="O0305_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCY0274332.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(6678..6926)
                     /locus_tag="O0305_001184"
     CDS             complement(6678..6926)
                     /locus_tag="O0305_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCY0274333.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            7034..7987
                     /locus_tag="O0305_001185"
     CDS             7034..7987
                     /locus_tag="O0305_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274334.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTK
                     QHVFHFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTKLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVELEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            7977..9356
                     /locus_tag="O0305_001186"
     CDS             7977..9356
                     /locus_tag="O0305_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCY0274335.1"
                     /translation="MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            9509..10969
                     /gene="ebpS"
                     /locus_tag="O0305_001187"
     CDS             9509..10969
                     /gene="ebpS"
                     /locus_tag="O0305_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCY0274336.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEA
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGA
                     AGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            11094..11210
                     /locus_tag="O0305_001188"
     CDS             11094..11210
                     /locus_tag="O0305_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274337.1"
                     /translation="MLFNKVFSRILAYQFNFLNSHQKDKTPQSTNLTRKNKK"
     gene            11371..12357
                     /locus_tag="O0305_001189"
     CDS             11371..12357
                     /locus_tag="O0305_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCY0274338.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKKQTPLES"
     gene            complement(12472..13440)
                     /locus_tag="O0305_001190"
     CDS             complement(12472..13440)
                     /locus_tag="O0305_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCY0274339.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            13517..14176
                     /locus_tag="O0305_001191"
     CDS             13517..14176
                     /locus_tag="O0305_001191"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCY0274340.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
     gene            14207..14323
                     /locus_tag="O0305_001192"
     CDS             14207..14323
                     /locus_tag="O0305_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274341.1"
                     /translation="MQRLTHIQNECADNDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         5634 a         2102 c         2485 g         4290 t
ORIGIN      
        1 tattgctact tcgtttcatg aatctaaagc tgataacaat attgagaata ttggtgatgg
       61 cgctgaggta gtcaaaagaa cagaagatac aagtagcgat aagtgggggg tcacacaaaa
      121 tattcagttt gattttgtta aagataaaaa gtataacaaa gacgctttga ttttaaaaat
      181 gcaaggtttt atcaattcaa agactactta ttacaattac aaaaacacag atcatataaa
      241 agcaatgagg tggcctttcc aatacaatat tggtctcaaa acaaatgacc ccaatgtaga
      301 tttaataaat tatctaccta aaaataaaat agattcagta aatgttagtc aaacattagg
      361 ttataacata ggtggtaatt ttaatagtgg tccatcaaca ggaggtaatg gttcatttaa
      421 ttattcaaaa acaattagtt ataatcaaca aaactatatc agtgaagtag aacgtcaaaa
      481 ttcaaaaagt gttcaatggg gaataaaagc taattcattt atcacatcat taggtaaaat
      541 gtctggacat gatccaaatt tatttgttgg atataaacca tatagtcaaa atccgagaga
      601 ctattttgtt ccagacaatg aattaccccc attagtacac agtggtttca atccttcatt
      661 tattgcaact gtttctcatg aaaaaggctc aggagataca agtgaatttg aaataacgta
      721 tggcagaaat atggatgtta ctcatgctac tagaagaaca acacactatg gcaatagtta
      781 tttagaagga tctagaatac acaacgcatt tgtaaacaga aattacacag ttaaatatga
      841 agtgaactgg aaaactcatg aaattaaagt gaaaggacat aattgatatg aaaaaaatag
      901 tcaaatcatc agttgttaca tcaattgcat tgcttttgct atccaataca gttgatgcag
      961 ctcaacatat cacacctgta agtgagaaaa aggttgatga taaaattact ttgtacaaaa
     1021 caactgcaac atcagattcc gataagttaa aaatttctca gattttaact tttaatttta
     1081 ttaaagataa aagttatgat aaagatacat taatactcaa agctgctgga aacatttatt
     1141 ctggctatac aaagccaaat ccaaaagaca ctattagttc tcaattttat tggggttcta
     1201 agtacaacat ttcaattaat tcagattcta atgactcagt aaacgttgta gattatgcac
     1261 ctaaaaatca aaatgaagaa tttcaagtac aacaaacggt aggttattct tatggtggag
     1321 atattaatat ctctaacggc ttatcaggtg gaggtaatgg ttcaaaatct ttttcagaga
     1381 caattaacta taaacaagaa agctatagaa ctagcttaga taaaagaact aatttcaaaa
     1441 aaattggttg ggatgttgaa gcacataaaa ttatgaataa tggttgggga ccatatggca
     1501 gagatagtta tcattcaact tatggtaatg aaatgttttt aggctcaaga caaagcaact
     1561 taaatgctgg acaaaacttc ttggaatatc acaaaatgcc agtgttatcc agaggtaact
     1621 tcaatccaga atttattggt gtcctatctc gaaaacaaaa cgctgcaaaa aaatcgaaaa
     1681 ttactgttac ttatcaaaga gaaatggata gatatacaaa cttttggaat caacttcact
     1741 ggataggtaa taattataaa gatgaaaata gagcaactca tacatcaatt tatgaagttg
     1801 attgggaaaa tcatacagtt aaattaatag atactcaatc taaggaaaaa aatcctatga
     1861 gctaaacaga tagataatca aaaaatctta aatatgttaa aatttacaaa cactttcttt
     1921 ctatattagg gtaaccacgt cttaattgac gtggttattt tttcagggca aaaaaagggc
     1981 ggattattta aataaggaca aacacttgtg gaaaatttaa aaggttaaaa ataataaaga
     2041 acttggtata acaagggttt tatacatttg cgtacaacga cgaaatgtca atttaccatc
     2101 acattatgac gatatgttta ttttaaacac acaagctcat gcgcgtcttg atcaaatggc
     2161 acaacagttt gaagttgttt gtaatggctt gaacgaaaat gaaggacaag caattcaaac
     2221 gatggatcaa tctgcctctc taatacggtc aaacttaatt caagttaaag aacaattaga
     2281 aaaactagct gtatactaat taatttatta aatgctactt gttttcattg agaataagta
     2341 gctttttcaa acataaaagt tttacaaaca cataaatggg tgatgagcta tgtttaaaag
     2401 aactaaacta atcttaatag caacattact gctatcagga tgttcaacta ccaataacga
     2461 atctaataaa gaaacaaaat cagtgccaga agaaatggaa gcttcaaaat atgtaggtca
     2521 aggcttccaa ccacctgcag aaaaagatgt ggttgaattt gcgaaaaagc ataaagataa
     2581 aattgccaaa cgaggcgaac aattttttat ggataatttc ggtctaaaag ttaaagctac
     2641 caatgttgta ggtagtggaa aaggcgtaga agtattcgtg cattgtgatg accacgatat
     2701 cgtatttaat gcgagtattc catttgataa atcaataatt gagagtgata gctcattaag
     2761 aagtgaggac aaaggcgatg atatgagtac tttagttggt acagtgttga gtggctttga
     2821 atatcgaaca caaaaagaaa agtatgacaa tttatataaa tttttcaaag ataatgaaga
     2881 gaaataccaa tatacaggct ttacaaaaga agcaataaac aagacgcaaa atgtcggata
     2941 taaaaatgaa tatttttata tcacttactc gtcaagaagc ttaaaagaat atcgtaagta
     3001 ttacgaacca ttgattcata aaaatgataa agaatttaaa gaaggaatgg aacaagccag
     3061 aaaagaagtg aattacgctg ctaatacaga tacagtaaca acattgttta gtacaaagga
     3121 aaattttact aaagacaata cagttgatga tgtaattgaa ctgagtgata aactatataa
     3181 ttttaaaaat aagccagaaa aatctacaat tacaattcaa ataggaaagc ctactattaa
     3241 tactaaaaaa gccttttatg atgataatga tccaatagaa tatggagtgt atcgtaaaga
     3301 tgaataaatt tttttaaaat gctttttaat attctatata aacacataaa tgggtgatga
     3361 gctatgttta aaaaggcgaa attaatctta atagcaacgc tactgttatc aggatgttca
     3421 gcgatgaata atgaatcaaa aaaagacaca aatacagaaa caaatacaga aacaaaatca
     3481 gtaccagaag aaatggaagc ttcaaaatat gtaggacaag gctttcaacc acctgcagaa
     3541 aaagatgcga ttgaatttgt gaagaagcat cgtaaagaat ttgaaaaagt aggtgaacaa
     3601 ttctttaaag ataactttgg actaaaagtt aaagctacaa atgttgtagg taaagatgat
     3661 ggtgtagaag tttatgtgca ttgcgaagat catggcattg tatttaatgc aagtctacct
     3721 ttgtacaaag atgccatcca tcaaaaagga tcaatgcgta gtaatgacaa cggtgatgat
     3781 atgagtatga tggtgggtac agtgctgagt ggctttgaat atcgagcgca aaaagaaaag
     3841 tatgataatt tatataaatt tttaaaagaa aatgaaaagc aatatcaata tacaggtttt
     3901 actaaagaag caattaacaa gacgcaaaac gtcgggtatc aaaatgaata tttttatatt
     3961 acatatttat caagaaattt aaaagaatat agaaaatatt acgaaccatt gatacataaa
     4021 aatgataaag agtttaaaga aggtatgcaa cgagctagaa aagagctaaa ctatactgct
     4081 aatacaaata ctgtagcaac gttgtttagt acgaatgatg aaaggaatag aaaagaaaag
     4141 ataaataatg taatagattt atccgagaaa attgaaagaa caaaagatat gccaatcaag
     4201 aatactataa ctactcaatt aggaaataaa cttattggca caaaaaaagc tcgttttgat
     4261 gataagaaag tagtgtcgtt tggagcattt gaagatgaat aaaataaatg atagagattt
     4321 aacagaattg agtagctata gggtttatca agacatcaat aaagataatg actttacagt
     4381 taacgaaaaa cgatttaagc aggcagatgt atttgaagat ttatatagag agaaactaaa
     4441 agacacaaat aaattaagag agtataatta tttacaaaat gaaactttta aaagcgcata
     4501 aataggtgat gagatatgct taaaaaagca aaatttatct taatggcaac gatactacta
     4561 tcaggatgtt caactaccaa taacgaatcc aacaaagaaa caaaatctgt accagaagaa
     4621 atggatgctt caaaatatgt aggacaagga ttccaaccac ctgcagaaaa agatgcgatt
     4681 gaatttgcaa agaagcataa agataaaatt gctaagcgag gcgaacaatt ttttatggat
     4741 aacttcggtc taaaagttaa agctacaaat gttataggta gtggcgatgg tgtagaagta
     4801 ttcgtgcatt gtgatgacca cgacatcgta tttaatgcga gtattccatt tgataaatca
     4861 attattgaca gtgatagctc attaagaagt aaggataaag gtgatgatat gagtacttta
     4921 gttggtgcag tactcagtgg gtttgaatat cgagcacaaa aagaaaaata tgataaatta
     4981 tataaatttt tcaaagataa tgaagagaaa tatcaatata caggatttac aaaagaagca
     5041 attaataaga cgcaaaatag tggttatgaa aatgaatatt tttatatttc ggccatacct
     5101 tataatttag ctgagtatag agactatttt gaaccattgt taaacaaaag tgacagtgaa
     5161 ttttcaaaag aattgtcaaa tgttaagaag caattaaaag ataagtctaa agtttcggta
     5221 actactactc tatttagtaa aaaaaagaac tatactaaaa aaagtaacag tgaaaatgta
     5281 ataaaaatgg cagaagaaat aaaaaaagat aaagagatac caaacggtat agagcttagt
     5341 ataaaatttt cggacaataa aataaatacg gttaaaccaa attttaacgg tgaaagcact
     5401 tcagaatatg gtgtgtttga tcaagaataa aattaatgat gaaaatttaa cggagaatag
     5461 tgtatattga gtagatcaag aataaaaaga taattctact attgttgtga aggcaaataa
     5521 gtagaagatt ttaagtgtaa tttctggtga tttaaataat aatataaatg gaagtactga
     5581 tataaaactt tttaacctac tagattctta taatttgctt tccattttat gacgattttt
     5641 actcaattga gtgatagaat caaaaaagcc atctcaaaaa ttaatcaagc aaacaacatt
     5701 ccaaacaatg ctcgcaaatc accaatgtat cactctccaa ttacgtaact atgatttaat
     5761 ttaagcatag ttattgaggt tttgtgatat atagtataaa attaatgaga attaaattta
     5821 ataatgtaaa attcatcttc ggggtcgggt gtaattccca accggcagta aataaagcct
     5881 gcgacctgct agtatgtatc atattagtgg ctgatctagt gagattctag agccgacagt
     5941 atagtctgga tgggagaaga tggaggtttt ttgttgtgca ataatcctcc tattcttacg
     6001 agatgaatgg aaggagaaaa ttgaatatgc aacaaaataa acgtcttatc acaataagta
     6061 tgttgagcgc gattgcgttt gtgttaactt ttatcaagtt tcctatacca tttttgccac
     6121 catacttaac tttagatttt agtgatgtac cgtcactact agctacattt acgtttggac
     6181 cagttgccgg tatcgtagtt gcactggtta aaaatttatt gaactactta tttagtatgg
     6241 gcgatccagt tggaccattt gctaactttt tagcaggcgc aagtttctta ttaactgctt
     6301 acgccatcta taaaaataaa cgttcaacaa aatctttgat tactggatta atcattgcaa
     6361 caatcgttat gactatcgtg ttgagtattt tgaactattt cgttctatta cctttgtacg
     6421 gtatgatatt taacttagct gatatcgcaa ataatcttaa agtaatcatt gtttcaggaa
     6481 ttataccatt caatattatt aaaggtatcg ttatttctat tgtatttatt ttactatata
     6541 gaaggcttgc gaatttcttg aaaagaattt aatcaaatta aagcaaaata atatacacat
     6601 aataataaaa agcaggtgac tatcaataaa cgatagcttg cctgcttttt ctatagaaca
     6661 tttgtctaaa aaatcaatta ttcaaatttt aaagcgtccc catcaaatga ttcgtctgca
     6721 attttaatag aatctgtagg gcatccatca attgcatctt ccatatcttc atataattcc
     6781 tcaggtactt ctgcagtacc ttggttatcg tcaaggatta cgaaagcaat accttcgtcg
     6841 tcgtaatcat atatatctgg cgctgctgca ccgcatgcac cacatgcaat acaagtatcc
     6901 atatcaacga ttgtatattt tgccaatgtc ttcgcctcct ttgataaaaa tgctaaaata
     6961 gtaatgtgac taaaatttta gacagcatca tttttatttt caaattatcc gttttacaga
     7021 gtgagggtta aatttgcaac acattataaa aacagcatta caacaaacat ttaactataa
     7081 aacaaataaa agtatttaca atatcttagt tggtaagaaa tctcaccaaa ccttttttga
     7141 cgcttgtagt caacaacagt tgtcattata tcacagttta ccactattaa aatatccgtc
     7201 ttttgagcta tttttagaaa aaatcaatga atttaatgct gaaatggaaa tcatgttgca
     7261 tcctagatat acatttgaaa gcatggggca aacatttcaa gcaattcaac tattagtgca
     7321 aaccatgtct aataccaaac aacatgtttt tcattttgta ccaatctctc aaaataataa
     7381 gatacaagaa actgtaaaga ttgtctataa ctacattaaa gaaaaccaat tacaaattga
     7441 ttttgaaaat gaactacata acttgtttaa agcaataact ttaaaagggc catgttactt
     7501 acattattat ttgcaaggct atgatgaacc aatgtatacg agacagcaag ttagtttaat
     7561 agaaaagcta tctcaacagc aattgtttga atacgaaatg aataatttag tgacaatgat
     7621 gtttgaatta gaaagtggag aatatactat tttatcaaaa ataataatga aacctacatt
     7681 attaaatcaa acttatatta cttatacaaa attgcttgaa caattcacga tggaagatat
     7741 agcggctcaa caacaagtta aaatcaatac tatcgaagat catgtacttg aaatcttaat
     7801 caaaggttac atgtctaatt acgatgatta tgttgaacta gaagatcaac tccagttttt
     7861 gaatttttat caacagcatc gtggcgaacg attaaaattt tacaaagaac aatttgacac
     7921 gttatcatat tttcaattaa aagtattaat cgttggattt gaaagaggtg atctgaatgt
     7981 tgcatgatat tttacgaaac aaatttggat tcgagagttt taaaccggga caacaggaaa
     8041 ttatagaaag tataatgtct caacaacaca ctctaggtat acttccaact ggaagtggaa
     8101 agagtttgtg ttatcaaata cctacgtatt tatcaggtaa gccgacatta attatctcac
     8161 cgttaatatc tttaatggat gaccaagtta tgcagttgaa aataaatgga gaaaaacgtg
     8221 taacatgtat tcactctggt atggatgaaa ttgagaaaaa gcataatatt aaatgtttac
     8281 gacatagccg cttcatcttt ctaagtccag aatttctcct gcaaccgtca aattttaaat
     8341 taatatctat gatagacttt ggcatgattg ttctagatga agcacattgc ctatctgaat
     8401 ggggatatga tttcagacca cattatgctc taataggaaa agtaacaaag cattttaaag
     8461 aagcggttgt cttagcattg acagcaactg caccaccgca tttacaagat gatttgacgg
     8521 aaatgttagc gattcaattc aatgttatta aaactacaat gaatcgccca aatataagct
     8581 ttaagcatct taattttcat gatgatgaag ataaaattga atggttgctg ccgtttctac
     8641 aacagtcggg accaacgatt atttatgtct catcgaaaaa gatgtgtctg aatttagcgc
     8701 aacttattta tgattcaggt tttcttacag gtatttatca tggtgatatg aattatcaag
     8761 agcgacacac agttcaacaa caatttttaa ataatgatat tccgattata gtcgcaacga
     8821 gtgcttttgg tatgggaatt aataaaaaag atattcgcac aatcattcac tttcatcttt
     8881 caacaagtcc ttctaactac attcaagaaa ttggccgtgc gggtcgcgat ggtgaactaa
     8941 gtcaggcaat tagtttattc caaccggacg ataaatatat tttagaaacg ttattatttg
     9001 cagatatgat aacagaagaa gatgtacaaa atttcgaaat aggagaattt ttagctcccg
     9061 ataaacaagc cgttttgaca acgttgcaat cattctatag tatcggcgcc ttgaaacaga
     9121 tatttaagca atcatttaaa cgaaagcaat taggattctt tcgcatgatt ggctattgca
     9181 aattggatca atgtagacgg aagtatttat tagaattttt cggtgaatat ccaccggcac
     9241 aagatcgatg ttgtgacaat gattctaata taactgatat cgcaatttta aataagaaga
     9301 aggtaattag aagtattgga tttgatgaaa agttgcaaaa tttatttctc agatagtatt
     9361 actttactaa aagaaaattg acaagctata attagtgtat acacaattga aaaatgattg
     9421 aaataatttt gaaaaatata cataaacata tgtcatgtgg gtatatttta tgtaaaatca
     9481 ttgtaataga atagaaagga agatggctat gtctaataat tttaaagatg actttgaaaa
     9541 aaatcgtcaa tcgatagaca caaattcaca tcaagaccat acggaagatg ttgaaaaaga
     9601 ccaatcagaa ttagaacatc aggatacaat agagaatacg gagcaacagt ttccgccaag
     9661 aaatgcccaa agaagaaaaa gacgccgtga tttagcaacg aatcataata aacaagttca
     9721 caatgaatca caaacatctg aagacaatgt tcaaaatgag gctggcacaa tagatgatcg
     9781 tcaagtcgaa tcatcacaca gtactgaaag tcaagaacct agccatcaag acagtacacc
     9841 tcaacatgaa gaggaatatt ataataagaa tgcttttgca atggataaat cacatccaga
     9901 accaatcgaa gacaatgata aacacgatac tattaaaaat gcagaaaata acactgagca
     9961 ttcaacagtt tctgataaga gtgaagctga acaatctcag caacctaaac catattttac
    10021 aacaggtgct aaccaatcag aaacatcaaa aaatgaacat gataatgatt ctgtaaaaca
    10081 agatcaagat gaacctaaag aacatcataa tggtaaaaaa gcagcagcta ttggtgctgg
    10141 aacagcaggt gttgcaggtg cagctggtgc aatggctgct tctaaagcta agaaacattc
    10201 aaatgacgct caaaacaaaa gtaattctgg caaggcgaat aactcgactg aggataaagc
    10261 gtctcaagat aagtctaaag atcatcataa tggcaaaaaa ggtgcagcga tcggtgctgg
    10321 aacagcaggt ttggctggag gcgcagcaag taaaagtgct tctgccgctt caaaaccaca
    10381 tgcctctaat aatgcaagcc aaaaccatga tgaacatgac aatcatgaca gagataaaga
    10441 acgtaaaaaa ggtggcatgg ccaaagtatt gttaccatta attgcagctg tactaattat
    10501 cggtgcatta gcgatatttg gaggcatggc attaaacaat cataataatg gtacaaaaga
    10561 aaataaaatc gcgaatacaa ataaaaataa tgctgatgaa agtaaagaca aagacacatc
    10621 taaagacgct tctaaagata aatcaaaatc tacagacagt gataaatcaa aagaggatca
    10681 agacaaagcg actaaagatg aatctgataa tgatcaaaac aacgctaatc aagcgaacaa
    10741 tcaagcacaa aataatcaaa atcaacaaca agctaatcaa aatcaacaac agcaacaaca
    10801 acgtcaaggt ggtggccaaa gacatacagt gaatggtcaa gaaaacttat accgtatcgc
    10861 aattcaatac tacggttcag gttcaccgga aaatgttgaa aaaattagac gtgccaatgg
    10921 tttaagtggt aacaatatta gaaacggtca acaaatcgtt attccataat ataactatat
    10981 aaattgtaac tgaactgcta tatacaaacg tgcatattat aagtatctct aaatattttg
    11041 gagaacttaa attgcgctta taagtatgta gcggtttttt catttttcaa agtttgttat
    11101 ttaacaaggt cttttctcga atattggcat atcaatttaa ctttttaaat agtcatcaaa
    11161 aagataaaac accacaatca acaaatttaa cgaggaagaa taaaaaataa atcaacatat
    11221 taaattgtag tgttattcaa ctccgtagct aacaattctc tatgcacatt aaacaaattg
    11281 tcaaaaatat atcataaatc ttcaagcaca gacttagcgc atcaatcact gaactgttat
    11341 aatagtttgg gattaaagga ggccgaaaca atgcaaaaag ttgaaagtat cataattggt
    11401 ggagggccat gcggattaag tgcggctatt gaacaaaaaa gaaaaggtat tgatacctta
    11461 attattgaaa agggtaatgt cgttgaatca atctacaatt atcctactca ccaaacattt
    11521 ttctcatcaa gtgataaatt aagtattggg gacgtaccgt ttatcgttga agaaagtaaa
    11581 ccaagacgta atcaagcgct agtttattac cgagaagttg taaaacatca tcaattaaaa
    11641 gtaaatgcat ttgaagaagt attaactgtt aaaaaaatga ataataaatt tactattact
    11701 acgacgaaag atgtttatga atgtcgattt ttaacaatcg cgacaggcta ttatggtcag
    11761 cataatacat tagaagttga aggtgcggat ttacctaaag tgttccatta ttttaaagag
    11821 gcacatccgt attttgatca agatgttgta attatcggtg gtaagaattc ggctatcgat
    11881 gctgctttgg agttggaaaa agctggtgct aacgtgacgg ttctatatcg tggtggagat
    11941 tattcgcctt caattaaacc gtggatactt ccaaatttca cagcattagt aaatcatgaa
    12001 aaaattgaca tggaatttaa tgctaatgtt acccaaataa ctgaagatac tgtgacttat
    12061 gaagtaaatg gtgaaagtaa aacgatacac aatgattatg tatttgcgat gattggttat
    12121 catcccgatt atgaattttt aaaatctgta ggcattcaaa ttaatacaaa tgaatttgga
    12181 acagcgccta tgtataataa agaaacatac gaaacaaata tcgaaaattg ctatattgca
    12241 ggtgtaattg ctgcagggaa cgatgcgaat accattttta ttgaaaatgg taaattccac
    12301 gggggcatta ttgctcaaag catgctagct aagaaacaaa cgcccttaga atcataaaaa
    12361 taaaggtcta tgtactaaat aacttagttt tacaacgact gacattcatg atatgtcaaa
    12421 tgaggttgat gactaatgat tgtacataga cctttttatg ttacgtattc attataattc
    12481 aaaatatgat ttgatttcag ctttatctaa attgttgctt aacgcgacta ataattttaa
    12541 tcttgctttt ggaccattca agccgttaga aaaaataaaa ccttgttgtg cgagttggta
    12601 accaccacca tcgtatgcgt aagttggact cacaatacca ttaaaggaac gtgaaactag
    12661 cacaataggt atatttaaag atactaattg ttgaatgcct tctaatgcgc ttggaggtat
    12721 gttgccttgt cctaacgctt caataaccat accatcgata ccttctcgac tataaaaact
    12781 aaaaatgtca cctggcatac ccatatatgc ttttactaat ggtacattta gtttgtcatt
    12841 gacattttcc aatgcttgtt ggcgatatgg catatgatgg aattgcacac gatcctttgt
    12901 caatacacct agcggaccat gatttggact ttgaaatgtg tttgtattag acgtatgtgt
    12961 tttggtaaca ttacgcgccg tatgaatttc atcattaaat acaaccatca cgcctttatg
    13021 acgggccttt tcatcagagg caacgcgaat agcggaaata taattatata atccgtcaga
    13081 accaatttca ttagacgagc gcattgcgcc agtaataaca acaggttgct cgatacctaa
    13141 tattaaatca agtaaaaagg cagtttcttc taacgtatcg gtaccatgcg tgataacgaa
    13201 accatcataa tatttatttg ttactgcttc taaaataata tcctttaatt gtttaacatg
    13261 ttggattgtc atatgaggtg atggtacatt aaaaggattt aattcatcta tttgtgcata
    13321 ttgatttatg acatcttgat gcattgaaat agggttgata tcatttgtta ctactttatt
    13381 agattggtct tgtgacatac taatggtgcc accagtatga ataacaagta gatgtttcat
    13441 atatttcctc ctatatttaa tttacctaat tatgataaaa tattattcat aaaacgacaa
    13501 ggaagggaaa tgacgcatga aagccattaa tattgcatta gatggtccag ctgctgccgg
    13561 aaaaagtaca attgcgaaac gtgtagccag cgaactatca atgatttatg tcgatacagg
    13621 agcaatgtat cgtgcattaa catacaaata tttaaaatta aacaaaactg aggactttgc
    13681 aaaactagtt gaccaaacaa cattagattt aacttataaa gcagataaag gtcaatgtgt
    13741 cattttagat aacgaagatg taacagactt tttaagaaat aatgatgtga cgcaacatgt
    13801 ttcatacgtt gcatctaaag agccagtacg ttcattcgcc gttaaaaaac aaaaagagtt
    13861 agctgcagaa aaaggtatcg taatggatgg tcgcgatatc ggaactgtag tgctaccaga
    13921 tgcagattta aaagtatata tgattgcatc agttgaagag cgagcagaaa gaagatataa
    13981 agataatcaa ttaagaggta tcgaatcaaa ttttgaagat ttaaaacgtg atattgaagc
    14041 tcgtgatcaa tatgacatga accgtgaaat atcaccatta agaaaagcag atgatgcagt
    14101 gacattagat acgacaggca agtcgattga agaagttact gacgaaattt tagcgatggt
    14161 gagtcaaatt aaataatcta ataagtcata actatctatt tgtaatgtgc aacgcttaac
    14221 gcatatacaa aatgaatgtg ctgataatga tttactcaaa ttaaaaggtg atttttattc
    14281 aatgatgaat gaaagttgcc tttttatttt tggtaaaagt taatgcgtca gtgaattgtg
    14341 taagtttttc aaaaagtaaa aagaaataat aaaggtgaat tattagaatt ccaaaaataa
    14401 ttcattacat tcataaagca ttttacaaat ggtaagaaaa tgagtgttac aaatctaaat
    14461 attgcaaaag aagctgattt agtcacaaaa aatgtcctat gtaataattc g
//
LOCUS       DAKTIP010000052        14370 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAKTIP010000052 DAKTIP010000000
VERSION     DAKTIP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.052"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(146..307)
                     /locus_tag="O0305_001193"
     CDS             complement(146..307)
                     /locus_tag="O0305_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274342.1"
                     /translation="MLLILELRFWNLEFGFRNSTFGIQNYNEWKILIIACLMTWFCSD
                     FYLMNCFFV"
     gene            complement(344..1084)
                     /locus_tag="O0305_001194"
     CDS             complement(344..1084)
                     /locus_tag="O0305_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCY0274343.1"
                     /translation="MFNLKHDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTMPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNVCHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISHFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
     gene            1290..2081
                     /locus_tag="O0305_001195"
     CDS             1290..2081
                     /locus_tag="O0305_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCY0274344.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2449..2877
                     /gene="lacA"
                     /locus_tag="O0305_001196"
     CDS             2449..2877
                     /gene="lacA"
                     /locus_tag="O0305_001196"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCY0274345.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2893..3408
                     /gene="lacB"
                     /locus_tag="O0305_001197"
     CDS             2893..3408
                     /gene="lacB"
                     /locus_tag="O0305_001197"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCY0274346.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3421..4353
                     /gene="lacC"
                     /locus_tag="O0305_001198"
     CDS             3421..4353
                     /gene="lacC"
                     /locus_tag="O0305_001198"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCY0274347.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHADIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4357..5337
                     /gene="lacD"
                     /locus_tag="O0305_001199"
     CDS             4357..5337
                     /gene="lacD"
                     /locus_tag="O0305_001199"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCY0274348.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5359..5670
                     /locus_tag="O0305_001200"
     CDS             5359..5670
                     /locus_tag="O0305_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCY0274349.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5676..7388
                     /locus_tag="O0305_001201"
     CDS             5676..7388
                     /locus_tag="O0305_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0274350.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7406..8818
                     /gene="lacG"
                     /locus_tag="O0305_001202"
     CDS             7406..8818
                     /gene="lacG"
                     /locus_tag="O0305_001202"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCY0274351.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYKGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIIDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9084..9953
                     /locus_tag="O0305_001203"
     CDS             9084..9953
                     /locus_tag="O0305_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274352.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIIMVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10236..11237
                     /locus_tag="O0305_001204"
     CDS             10236..11237
                     /locus_tag="O0305_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCY0274353.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11471..12472
                     /locus_tag="O0305_001205"
     CDS             11471..12472
                     /locus_tag="O0305_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0274354.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISTNPAENEGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEIPLVDYIF
                     CTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHRTP
                     DMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVINI"
     gene            12779..14341
                     /locus_tag="O0305_001206"
     CDS             12779..14341
                     /locus_tag="O0305_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0274355.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
BASE COUNT         5014 a         2135 c         2765 g         4456 t
ORIGIN      
        1 attctgaaaa atataatgat ttaatatcgg aatttgataa tgaagatatc taatttttta
       61 atattcgtag cttttatttt tctacttatt ttatgtttat ttttaatctt acaaatgaca
      121 aaccattaaa agtagcatcc caacatcaaa caaaaaaaca attcatcaaa taaaaatcgc
      181 tacaaaacca agtcattaaa cacgcaataa ttaaaatttt ccactcatta taattctgaa
      241 ttccaaatgt cgaattccga aaaccaaact ccaaattcca aaaacgcaac tccaaaatta
      301 aaagcatttc cctaccattc gggaaatgct ttttacatac tgattactct gtcattaatg
      361 attttacaac ggaaaccatg tcgtcatgta tgaccaaagt agcgtcgcta tcataaggtg
      421 ttcgatcttt attgataata attaaattgt cgcctttaaa atgtgatatt aatcctgcgg
      481 caggttgtac aacgagtgat gaacctagta caacaagggt gtcagcatgt tcaattttat
      541 ttaatgccct tatgatggta ggttgatcta acatttcacc gtataatacg atgtccggtc
      601 gaatggcacc accacaatta tcacaatgtt tcaaagttct atcaataaca tctgacttcg
      661 tataagattt atgacataca ttacaataaa aacgatttaa cgtgccatgt aattcatcaa
      721 catgttgact tccagcgtct gagtgcaaac catcgatatt ttgcgtgatg acacctaaag
      781 attgttgatt acgttctaat tttgcaatcc aatcatgaac gatattgggc atcgtatcga
      841 caaatagtaa gcgcttatgg cagaaattga taaaaccttc aggatcatct tctaaataat
      901 cacggcttaa caagtattct ggcgaaagcc catcttttga aatttcatca aataagccac
      961 ccattgaacg gaaatctgga acgccacttg cgacagatac accagcacct gtaaaaaatg
     1021 taatacgatt cgaactatct ataatatgtt ttagtgtctc taaatcatgt ttcaagttaa
     1081 acaccccatc aaaataatga atgatttata acgtctttta cttttattat agtatattca
     1141 ttttacgatt atcaaaagcg cagtttaaac agttcttttt aaaaatgaat tatacatata
     1201 atcgaacagt taaattatat tagtttttac gcaattttta ttatagaata agcatgtata
     1261 ttaaaattga tgtctaaaca taaaggataa tgacccatcg cctaaataaa ggaggcaatt
     1321 ttacattgaa taaacatgaa cgtttggacg aaattgctaa actagtgaat aaaaagggca
     1381 cgataagaac gaatgaaatc gtcgaaggtt taaatgtgtc tgatatgaca gttcgaagag
     1441 atttaattga attggaaaat aaagggattt taacgaagat tcatggtggt gcacgcagta
     1501 attcaacatt tcagtataaa gaaatatcgc ataaagaaaa acatacccgt caaattgcag
     1561 aaaaacgatt tatagctaaa aaagctgcat cattaattga agatggggat actttgtttt
     1621 tcggaccagg aacaacagtg gaactattag cagaagaagt caatcatcat acgctcacaa
     1681 ttattacgaa ttgtttgccg gtgtataaaa ttttgttaga aaaacaaaca gcacattttc
     1741 gtgtctattt aattggtggt gaaatgcgcc atattacaga agcatttgta ggtgaaatgg
     1801 cgaacgccat gttggaaaaa ctaagattta gcaagatgtt ctttagtagt aacgcagtaa
     1861 ataaaggtgc tgtgatgaca tctacattag atgaagccta tacgcaacaa ctggcactaa
     1921 gtaattcaat tgaaaaatac ttgttaatcg atcatacgaa agttggcaaa gaagatttta
     1981 catcattttg tcagttaaac gaattgactg cagtggtcat ggactatgaa gatgaagaaa
     2041 aagtagaaac gattaaaaca tatattgaag tagtcgacta aattaaaagt ataactgcat
     2101 tgttaaaata tgtatctata tgctcataag tgaatgtgaa tttgcattca tttatgggct
     2161 attttttatg caaatttatg tcaacgggaa cctcgacaaa gttttactac aatttcagac
     2221 gaatacctaa ttaaaacaac accacaatat gaatgttcac ttattgtttg aaaatacgtc
     2281 ataaacaaac atattataaa catattatgt tgatttattg tttgtttatg tttataattt
     2341 aaacgtaatc aaattgaaag ccttttctca agattttcat aacctacaaa taaatatgtc
     2401 acattttaag aaaggtattt caaaattaaa gtaaaaagga gtcttattat ggcgattatt
     2461 attggttcag atgaagctgg caaacgatta aaagaagtca tcaaatcata cttattagac
     2521 aataaatatg atgttgttga cgtaacagaa ggacaggaag ttgactttgt tgatgcaact
     2581 ttggctgtag caaaagatgt tcaaagtcaa gaaggtaact taggtattgt tatcgatgca
     2641 tttggtgcag gtagctttat ggttgctaca aaaatcaaag gcatgattgc tgctgaagta
     2701 tcagatgaac gctctggata tatgactaga ggccataaca attcaagaat gatcactatg
     2761 ggatctgaaa ttgttggaga cacattagcg aaaaatgtcg ttaaaggttt tgttgaaggt
     2821 aaatacgatg gtggtagaca ccaaatccgc gtagatatgc ttaataaaat gtgctaagga
     2881 ggaaaaatta ctatgaagat tgcattagga tgcgaccata ttgttacaga tacaaaaatg
     2941 cgtgtatctg aatttttaaa atcaaaagga catgaagtca ttgacgtagg aacatacgat
     3001 ttcacaagaa cacattatcc aatttttggt aaaaaagttg gcgaacaagt tgttagcggt
     3061 aatgcagact taggtgtttg tatttgtgga acaggtgttg gtattaacaa tgctgtaaat
     3121 aaagtacctg gcgttcgttc agcactagta cgtgatatga catcagcgtt atacgctaaa
     3181 gaagaattaa atgcgaacgt tattggcttc ggtggacgta ttataggtga gttattaatg
     3241 tgcgatatta tcgatgcatt tattaatgct gaatataaac caactgaaga gaacaaaaaa
     3301 ttaatcgcta aaattaaaca tttagaaaca agcaatgcag atcaagctga tccacatttc
     3361 tttgatgaat tcttagaaaa atgggacaga ggcgaatacc acgattaagg gacgacacat
     3421 atgattttaa ctttaacatt aaatccatcc gttgatattt catacccatt aacagcattg
     3481 aaattagatg atgttaatcg tgttcaagaa gtaagtaaaa cagctggtgg taaaggttta
     3541 aatgtaacaa gagtacttgc gcaagtaggt gagccagtcc ttgcgagtgg ttttattggc
     3601 ggtgaattag gtcaatttat tgctaaaaaa ttagatcatg ccgacatcaa gcatgcattt
     3661 tataacatta agggtgaaac acgaaactgc attgcaattt tacatgaagg gcaacaaaca
     3721 gaaattttag agcagggacc ggagattgat aatcaagagg ctgcaggatt tattaaacat
     3781 tttgaacaat tattagaaaa agttgaagca gttgctattt caggtagctt acctaaagga
     3841 ttaaatcaag attattatgc acaaatcatt gaacgatgcc aaaacaaagg tgtgcctgtg
     3901 atattagatt gctcgggtgc aactttgcaa acagttttgg aaaatccata caaacctaca
     3961 gtcattaaac caaacatttc tgaactatat caattgctta accagccact tgatgaaagt
     4021 ttagaaagtt tgaaacaggc agtgtcacaa cccttattcg aagggattga gtggattatt
     4081 gtgtctttag gtgcacaagg tgcattcgct aagcacaatc atacattcta tagagtgaat
     4141 attccgacaa taagtgtatt aaatccggtt ggttcaggtg attctacagt tgctggaatt
     4201 acttcagcaa ttcttaatca tgaaaacgac cacgatttac taaaaaaagc caatacttta
     4261 ggcatgctga atgcacaaga agcacaaaca ggttatgtca atttaaataa ttatgatgat
     4321 ttattcaatc aaattgaagt tttagaggtg taaaaaatgt caaaatcgaa tcaaaaaatc
     4381 gcatcaattg aacaattaag taacaacgaa ggtatcattt ctgcattagc atttgaccaa
     4441 cgtggtgctt taaaacgcat gatggcaaaa catcaaactg aagaaccaac tgtagctcaa
     4501 attgaacaat taaaagtatt agttgcggaa gaattaacac aatatgcatc ttcaatttta
     4561 ttagatcctg aatatggttt accagcttca gatgcacgta acaaagattg tggattatta
     4621 cttgcttatg aaaaaactgg atatgacgta aacgctaaag gtcgtttacc tgactgttta
     4681 gtagagtggt ctgcaaaacg tttaaaagaa caaggcgcaa atgctgttaa attcttactt
     4741 tactatgatg ttgatgatgc tgaagaaatt aacattcaaa agaaagcata cattgaaaga
     4801 atcggttcag aatgtgttgc agaagatatt ccattctttt tagaagtatt aacatacgat
     4861 gacaacattc ctgataacgg tagtgttgaa ttcgcaaaag ttaagcctag aaaggttaac
     4921 gaagcaatga aattattctc agaaccacgc tttaacgttg atgtattgaa agttgaagta
     4981 ccagtaaaca tgaaatatgt agaaggcttt gctgaaggtg aagtggttta tacaaaagag
     5041 gaagcggcac aacactttaa agatcaagat gcagcaactc atttaccata catttactta
     5101 agtgctggtg tttctgcgga attattccaa gaaactttaa aatttgctca cgaagctggt
     5161 gctaaattca acggggtact ttgtggccgt gctacatggt ctggtgctgt tcaagtatac
     5221 attgaacaag gtgaagatgc tgctcgtgaa tggttacgta caactggttt caaaaatatt
     5281 gatgacttaa ataaagtatt aaaagatact gcgacatctt ggaaacaaag aaaataagtt
     5341 taaaagggga ttaatgctat gaatagagaa gaagtccaat tattaggttt tgaaattgtt
     5401 gcatttgcag gggatgcacg ttctaagttt ttagaagcat taacagcagc tcaagctgga
     5461 gattttgcaa aagcagatgc actgattgaa gaaggaaaca attgcattgc ggaagcgcat
     5521 agagcacaaa caagcctgtt ggctaaagaa gcgcaaggtg atgatattgc atacagtgta
     5581 acgatgatgc atggtcaaga tcatttaatg acaacaattt tactgaaaga tttaatgaag
     5641 catttattag aattttataa aagagggtga tgacaatgat gcaaaaattg atcgcacaaa
     5701 ttgaaaaagg gaagccattt ttcgagaaat tatcacgtaa catatattta agagccatcc
     5761 gtgatggatt tatttctgca atgcctgtca tcttattctc aagtatcttt ctattaattg
     5821 catatgtgcc aaatatattt ggttttaaat gggataaagg catggaagcc attttaatga
     5881 aaccatacaa ctatacgatg gggcttgtag catttttagt agcaggaacg acagcgaaat
     5941 ctttaacaga ttcatttaac cgtaagctag aaagtacaaa tcaaattaac tttatttcta
     6001 caatgctagc agcgatgtgt ggattcttgt tcttagcttc tgatccagcc aaagatggtg
     6061 gattcttaag tgcatttatg ggaactaaag gtttattaac agcattctta tcagcatttg
     6121 taactgttat tgtttataac ttctgtgtga agcgcaatat tacaattaaa atgccgaaag
     6181 aagtaccgcc gaatatttca caagtattta aggacttaat tccattttca gcggtaatca
     6241 tcattcttta tgcattagat ttagtcattc gcaacagctt taaatcaaat gtagcggaag
     6301 gtattttaaa attattcgaa ccattattta cagcagcaga tggatggatt ggtgtcacaa
     6361 ttatctttgg tgcctttgca ttattctggt ttgtaggtat tcatggtccg tcaattgtag
     6421 agccagcaat tgcagccatt acatatgcga atatcgaagc gaacttcaag ttgcttcaag
     6481 ctggagaaca cgcagataaa attattacat caggtacaca aatgtttatc gttacatttg
     6541 gaggtacggg tgcaacattg gtcgtcccgt tcatgtttat gtggatgacg aaatctaaac
     6601 gaaacaaagc gattggacgt gcatcagttg taccaacatt ctttggtgta aacgaaccaa
     6661 ttttatttgg tgcaccacta gtattgaatc ctgtgttctt tattccattt gtattagcac
     6721 caattgttaa cgtatggatt ttcaaacttt tcgttgaagt gttaggaatg aatagtttta
     6781 gtgtgaattt accttggaca acaccaggtc cattgggcat tatcatgggt acaggttttg
     6841 gtttatggtc attcgtacta gctattactt tgattgttgt agatattatt atttactacc
     6901 cattcctaaa agtttatgat agtgaaattc ttgatgaaga agaaggacgt aaagaaagta
     6961 attcagattt aaaagaaaaa gttgcagcaa actttgatac gaaaaaagct gattcaattt
     7021 tagcggcaag tggtgtatca gacgatgcag ctaaagcttc aaatatcaca gaacaaacaa
     7081 atgtcttagt actttgtgcc ggtggtggta caagtggatt attagccaat gcacttaata
     7141 aagcagctga agaatatcat gtgccagtga aagcggcagc tggcggatat ggtgcacata
     7201 tggacattat gaaagagtac caattaatca ttttagcgcc acaagttgcg tctaactatg
     7261 aagatattaa gcaagataca gatagattag gtattaaatt agcgaagact caaggtgctg
     7321 aatatatcaa attgacacga gatggccaag cagcccttga tttcgttcaa caacaatttg
     7381 aaaattaaga ataggagttt ttcatatgac taaaacatta cctgaagatt ttatttttgg
     7441 tggagcaaca gcagcatatc aagcagaagg tgcgacaaat acggatggta aaggtcgcgt
     7501 agcatgggat acgtatttag aagaaaacta ttggtacact gcagaaccag cgagtgattt
     7561 ttataacaga tatccagtgg atttagaatt aagtgaaaaa ttcggtgtta atggtatccg
     7621 aatttcaatt gcatggtctc gtatcttccc aaatggatat ggcgaagtta accctaaagg
     7681 tgttgagtat taccataagt tatttgcaga gtgtcataaa cgtcatgttg aaccatttgt
     7741 aacattgcat cattttgata caccagaagt gttacacaaa gatggcgatt ttctaaatcg
     7801 taaaacgatt gactactttg tagattatgc tgaatattgt tttaaagaat tcccagaagt
     7861 aaaatattgg actacattca atgaaattgg acctattggt gatggtcaat atttagtagg
     7921 taaattccct ccaggtatta aatatgattt tgaaaaagta ttccaatcac atcataacat
     7981 gatggtggct catgctagag cagtaaaatt atttaaagat ggcggataca aaggagaaat
     8041 tggtgttgta cacgcattgc caacaaaata tccgttcgat ccatcaaatc ctgaagatgt
     8101 tcgtgcagca gaattagaag atatcatcca taataaattc atcttagatg caacatattt
     8161 aggtaagtat tctcgtgaaa cgatggaagg tgtgcaacat atcttatctg ttaatggcgg
     8221 taaattaaac attacagatg aagattatgc cattttagat gcagccaaag atttaaacga
     8281 cttcttaggt atcaattact acatgagtga ttggatgaga ggttacgatg gagaatctga
     8341 aatcacgcat aatgcgacag gtgataaagg cggttctaaa taccaactta aaggtgtcgg
     8401 acaacgtgaa tttgatgtcg acgtacctcg cacagactgg gactggatga tctatcctca
     8461 aggtttatat gatcaaatca tgcgtgttgt taaagattat cctaactatc ataagattta
     8521 tatcactgaa aatggtttag gatataaaga tgaatttatt gaatctgaaa aaacagttca
     8581 tgatgatgca cgtattgatt atgtaagaca acatttgaac gttattgcag atgctattat
     8641 agatggtgca aatgttaaag gttactttat ttggtcatta atggatgtgt tctcttggtc
     8701 aaatggttat gaaaaacgat atggtttatt ctatgtcgat tttgagactc aagaacgtta
     8761 tcctaagaaa agtgcttatt ggtacaaaga gttagccgaa acaaaagaaa ttaaataatt
     8821 atagatatta aaaggcctat cagtcgagtg ctggtaggtc tttatttata gaaagagata
     8881 aattaaacat gcatatacat atttatatat taatcaatgg gtgacacacc attgaaagct
     8941 aaagaagcaa tcaattaaca aacgttaacg gacaactttc caaagtcatc ttaaaagtta
     9001 atgatgtatt caaaatcaac taaaggacat ttttaatcac atcctttata cttatataaa
     9061 agacatgaat gggagttaaa agtgtgaaga aaatttttgt aattattacg acattgttag
     9121 cggtcgctat tattataggc tctattatta tggtggtgtt tagtcaacga caggcacaaa
     9181 catttaaaat acaacaacag caatttgtta aaaagccaat tccaacattg ttcttacatg
     9241 gatttggtgg tagtgccaat tctgaaaagt ttatggtgaa gcaagcagaa aaaagagggg
     9301 taactaaaga tatcatcact gcttacgttt caaaggatgg tgcagtgaca tttaaaggga
     9361 aactaagaaa agatgcagtg aatcctattg tgaaaattga acttgaaaat aacagacaag
     9421 gctatttgga taagaatgct gcgtggttta aaaatgtact gactaagtta caatctgaat
     9481 acaatttcga taaattcaac tttgtcggtc actcaatggg gaacttaaca tttgcacaat
     9541 atatgatgac atatggtaac gacaagtcac tgccacagtt aaacaaacaa gtgaatattg
     9601 cagggacatt taatggtgtt ttaaatatga atgaagatgt caatgaaatt acagttgata
     9661 aagatggtaa accaagtcga atgaatcaac cttatcagca attacgcgtg ctaaaagata
     9721 tttataaagg taaaggtatc gaagtgctaa atatttatgg ggatttgaaa gatggtacac
     9781 attcagacgg cagagtgtct aatagttctt cgaaatcatt aaaatattta ctgggaaata
     9841 gtccgaaaag ttatcgagaa tctaaatatg aaggtgagcc tgcacaacat agtcagcttc
     9901 atgagaatga aaatgttgct aatgagttaa tcgacttctt atggaaaaaa taaagcaact
     9961 gctagttttt ctggggcaaa aaagcttgta atattagagc ttttcaaaaa ttatctattg
    10021 tttgagagtt tccataaaat gtctaagtcg taacatttta aatttatgat tatagcaaat
    10081 ataatgtggt tgaagttaca tgaatgaatc agtgcttaag tcaaaatata acaactaaga
    10141 atgtatttgc atacagacat cacgaagaca caattcaaac ttaacgctta agtaacaaat
    10201 aagtaaaagt ttcaaataaa gaaaggtgat taatgatgca aaataaacaa attcttttta
    10261 acaaaatacc agaaggcatg cctcaagagg atacattcaa aattgaagag attgacacgc
    10321 ctaaacttga gagcgatggt gtacttgtac aaacgctgta tatttcagtg gatccatata
    10381 tgagaggacg tatgactaag gcagattcgt atgtacagcc ttttgaaatt ggtaaaccga
    10441 ttgttagcca tgtcgttgca aaagtaattg attcgacact cgcagattat aaaaaaggag
    10501 atgtcgttgt aggtatgttg ccttggcgaa tcattaatca tgtccaagcg gatcaaatta
    10561 ctaaagttcc aacgacagat gtaccacttg atttatattt aagtgtttta ggaatgccag
    10621 gacaaacagc atatcatggt ctgcttgata ttggacaacc taaagccggt gatactgttg
    10681 tggtttcagc tgcatctggt gctgtaggtt cagttgtagg tcaaattgct aagattaaag
    10741 gttgccgtgt agtaggtatt gcgggtggcg acaaaaaagt aaattattta acagagacac
    10801 tgggctttga cgctggtatt gattataaga aagaggattt tgcagaagcg ttagcacaag
    10861 cagtaccaga tggtattgat gtttattttg aaaatgttgg tggaacagtt ggcgatgaag
    10921 tatttaagca tttaaatcgt tttgcacgtg taccagtatg tggcgcaatt tcgtcatata
    10981 atcatcctga agccgatatc ggaccacgta ttcaaggtac gcttattaag aagcaagtaa
    11041 tgatgagagg cttcttggta agtgaattcg ctaatgcttt taaagaagct agtgaacagc
    11101 ttgcaacatg ggttcaagaa ggtaaaattc agtcacaagt aaccattgaa gatggttttg
    11161 aaaatgcgcc acatgcattt aaaaatttat ttactggcga taactttggt aaacaagtta
    11221 ttaaagtaac ggaataataa agtatacgtt ttaacaaact aactttgtta attgaccaaa
    11281 atttaataat tttttatcta aagtaaattt gttcagcaac atggtagaaa taagtctaaa
    11341 atttaatacg aaagtcgtct gtaattattt atatattcaa atgaaaaagg agtcatgatg
    11401 ctgcgatata agcacacatg gcttctattt tgtataataa gaaataagtt cataaatgag
    11461 gtgaaacgat atgaaaatga taggatttga gaagcccttt aaattggaag aaggaaattt
    11521 atttaaagta tacgaacaaa gaaagccaac gccagaaaac gatgatatct tagtcaaagt
    11581 aaatagcatt agtgtaaatc ctgtagatac aaagcaacga caaatggaag tcactcaagc
    11641 acctcgtgta cttgggtttg atgctattgg tactgttgaa gctatcggac cagacgttac
    11701 attatttagt ccgggtgacg ttgtatttta tgctggatcg ccaaatagac aaggatcaaa
    11761 tgcgacatat caattggtgt cagaagctat agttgcaaaa gcaccacaca acataagtgc
    11821 taatgaagcg gttagtctac cgctaactgg tattacggca tatgaaacgt tttttgatac
    11881 atttaaaatt tctacgaatc ctgctgaaaa cgaagggaag tctgtcttaa tcattaatgg
    11941 tgccggtggt gtaggcagta tagccactca aattgctaaa cggtatggtt taactgtcat
    12001 tacaacagca tcacgacaag aaacaacaga gtggtgtgaa aagatgggcg cagatatcgt
    12061 attgaatcat aaagaggatt tagtgcgtca atttaaagaa attccattag tagattatat
    12121 attttgcacc tataatacag acttgtatta caatactatg attgaactga taaaaccatt
    12181 agggcatatt acgactatcg tagcttttaa tgaagatcaa gatttaaatg cattaaaatt
    12241 aaaaagtata acatttacac atgaatttat gtttgcgcgt ccaattcatc gaacgcctga
    12301 tatgattaaa caacatgagt atttagaaga tattacgaaa aatatagaat cagggcatta
    12361 ccaaccaaca acgacacaag tttttgaagg tttatcacct gaaaacttat atcaagcaca
    12421 tcaattgttg gaaaaacaat caatgattgg aaaattagta attaatatat agattagtaa
    12481 gtaaagtcgc cttatgcaga aaatactgta taggcgactt attttttgtg tctagcgact
    12541 attgattcta aacggaaata tggcaacttt gcaggtatca aatcaataat gcactagaaa
    12601 ggcattactt aaatacaata aaatactttg ttgctcaatg ataaataatt tgagtgtgtt
    12661 ttatatatca tttttaaaag atgaggcatt attaattgtg tctatataaa attacaaatg
    12721 attaaaatgt tgcagttatg ggtatgaact taccattaca cagaaagggt ttgtatttat
    12781 gaaaaataaa tcagtaagtc tagtgttttg ggtatcttta gtgatatgta cgatatttgt
    12841 tgcttttgga gcaatatttc cgaaacagtt agaaaaactc acgcaaaaca ttactacatt
    12901 tattgcgcta catttttctt ggtattattt attgctcgtt ttagttattt tatttgtctg
    12961 tgtgtatatt ttattttcga gatacgcaaa cattacatta ggtgaagaag gcgaagatcc
    13021 tgaattctca ttgccgtctt ggtttgctat gttatttagt gctggtatgg gaataggact
    13081 tgtgttctgg acaacggcag aaccaatcag tcatgccttc aaattaacgc ctattcataa
    13141 agctggcacg caatctgcaa ttaatgatgc aatgcagttt tcatttttcc attgggggat
    13201 tcatgcatgg gcagtctatg gtattgtcgc gctagtgttt gcatatttta gctttcataa
    13261 aggatatcct ggcttagtca gcgctacatt gacgccttta ttaggtgaaa aggcaatgcg
    13321 tggaccttta ggtggtgcca ttgatgtatt ggcagtcata gctacagtaa caggcgttgc
    13381 tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt ttacattttt tatttaatgt
    13441 accgagtaac ttcacgatgc aagtaatctt gattgtcatt gcaaccatat tatttacttg
    13501 gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg agtaatatca acatgttact
    13561 tgcttttgta gtacttattg gtctttttat tgttgggcca acgctataca ttttaaatac
    13621 atttacgaat ggtctaggta attatattgc aaatttcttt agtatgagtt tacgcattcc
    13681 gacaggtggt caaaaattcc aatggttaca aaattggaca atcttttact gggcatggtg
    13741 gatatcttgg gcaccatttg taggtatatt tattgcacgt gtgtcaaaag gacgtacgat
    13801 taaagaattt attctaggtg tactttttgt cccggcgtta gtttgcttta tattctttgc
    13861 ggtattcggc gcgtcagcaa tttacttaca agataatcat attgccgata ttgctaaagc
    13921 tgcgacagaa acagcaactt ttgcgacgct acaacattat ccgctaggtt ttgtattaag
    13981 cttaattact ttacttgtta ttatgatttt ctttgttaca tcagcggatt cggcaactta
    14041 tgttttaggt atgttgagtt caagtggtga tattaatccg aagtcatttg tcaaagtgag
    14101 ttggggtatc attatggcat tatttgcaat catcatgatt tacacaggtg gtactcaagc
    14161 aattcaaaat ttattaatca ttgctgcgtt accattttcc gtggtcatta tagcgatgat
    14221 atggtcgctt ataaaatctt tgagtgagga gaaaccgaga aatagtaata aggttttaat
    14281 taaacatcgt gacccagata ttttagaata tcgttcttca aaacatattg acgaaaatta
    14341 attaataatg aggttttatt tacaaaaact
//
LOCUS       DAKTIP010000053        13878 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAKTIP010000053 DAKTIP010000000
VERSION     DAKTIP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.053"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            58..2163
                     /gene="clpL"
                     /locus_tag="O0305_001207"
     CDS             58..2163
                     /gene="clpL"
                     /locus_tag="O0305_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCY0274356.1"
                     /translation="MNNGFFNSDFDSIFRRMMKDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(2232..2402)
                     /locus_tag="O0305_001208"
     CDS             complement(2232..2402)
                     /locus_tag="O0305_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCY0274357.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(2415..4409)
                     /gene="feoB"
                     /locus_tag="O0305_001209"
     CDS             complement(2415..4409)
                     /gene="feoB"
                     /locus_tag="O0305_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCY0274358.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKYLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAEQVGAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(4421..4648)
                     /locus_tag="O0305_001210"
     CDS             complement(4421..4648)
                     /locus_tag="O0305_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCY0274359.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(4864..7353)
                     /gene="farE"
                     /locus_tag="O0305_001211"
     CDS             complement(4864..7353)
                     /gene="farE"
                     /locus_tag="O0305_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCY0274360.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            7498..8046
                     /gene="farR"
                     /locus_tag="O0305_001212"
     CDS             7498..8046
                     /gene="farR"
                     /locus_tag="O0305_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCY0274361.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(8123..8290)
                     /locus_tag="O0305_001213"
     CDS             complement(8123..8290)
                     /locus_tag="O0305_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274362.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISCF
                     NSDKHDKNKKR"
     gene            complement(8402..9946)
                     /gene="pruA"
                     /locus_tag="O0305_001214"
     CDS             complement(8402..9946)
                     /gene="pruA"
                     /locus_tag="O0305_001214"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCY0274363.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(10136..10735)
                     /locus_tag="O0305_001215"
     CDS             complement(10136..10735)
                     /locus_tag="O0305_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCY0274364.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            10974..11165
                     /gene="cwrA"
                     /locus_tag="O0305_001216"
     CDS             10974..11165
                     /gene="cwrA"
                     /locus_tag="O0305_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCY0274365.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            11405..13813
                     /locus_tag="O0305_001217"
     CDS             11405..13813
                     /locus_tag="O0305_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0274366.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLILTETTTFKAVPGHGIEATIDH
                     HYILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
BASE COUNT         4571 a         2559 c         2225 g         4523 t
ORIGIN      
        1 cgcttcaaaa gtaatttctt ttttgtttag ttcaaaaaaa tttagaggtg atgttatatg
       61 aataacggtt ttttcaatag cgactttgat tcaatttttc gaagaatgat gaaagatatg
      121 caaggttcaa atcaagtcgg aaacaaaaag tactatatta atggtaaaga agtttcacct
      181 gaagaactag cgcaactcac acaacaaggt ggcaatcact ctgctgaaca aagtgcgcaa
      241 gcttttcaac aagcagcaca aagacaacaa gggcaacaag gtggcaacgg caattattta
      301 gaacaaattg gtcgtaacct tacgcaagaa gcacgtgacg gtttattaga tccagtcatt
      361 ggtcgtgata aagaaattca agaaactgct gaagttttaa gtagacgaac taaaaacaat
      421 cctatattag ttggagaagc tggtgttggt aaaactgcga ttgttgaagg tttagcacag
      481 gcaatcgttg aaggaaatgt accagcagca atcaaagaca aagaaattat ttctgtagac
      541 atttcatcat tagaagctgg aacgcaatat cgtggtgctt ttgaagaaaa tattcaaaaa
      601 ttaatcgaag gtgttaaatc ttcacaaaat gccgtactat tctttgatga aatccatcaa
      661 attatcggtt caggtgccac aggaagtgat tcaggtagca aagggttatc tgatattttg
      721 aaacctgcat taagtcgtgg tgagatttct attattggtg caacaacaca agatgaatat
      781 cgaaacaata ttcttaaaga tgctgcatta acgcgcagat ttaatgaagt gcttgttaat
      841 gaaccaagcg ctaaagatac tgttgaaatt ttaaaaggta ttcgcgaaaa attcgaagaa
      901 caccatcaag taaaattacc agatgacgta ttaaaagcat gtgttgactt atcaattcaa
      961 tatattccac aacgattatt accagataaa gcaatcgatg tgttagatat tacagcagca
     1021 catttatctg cgcaaagtcc agctgtcgat aaagttgaaa ctgaaaaacg aatttctgaa
     1081 ttagaaaatg ataaacgtaa agcagtaagt gctgaagaat ataaaaaagc tgacgacatt
     1141 caaaatgaaa tcaaatcatt acaagataaa ttagaaaata gtaatggtga acatactgct
     1201 gttgctacag ttcatgatat ttcagatact attcaacgat taactggtat tccagtttct
     1261 caaatggatg ataacgatat tgaacgttta aaaaatattt ctaatcgttt aagaagtaaa
     1321 atcataggtc aagatcaagc tgtagaaatg gtttcacgtg caattcgccg taatcgtgct
     1381 gggtttgatg acggcaaccg tccaattggc agtttcctat ttgttggccc tactggtgtt
     1441 ggtaaaacag agcttgctaa acaattagca attgatttat ttggtaataa agatgcactg
     1501 attcgacttg atatgagtga atatagtgac acaacagctg tttcaaaaat gattggtaca
     1561 actgctggtt atgttggtta tgatgacaat tcaaatacgt taactgaaaa agtacgccgt
     1621 aatccatact cagtcattct atttgatgaa atcgaaaaag caaatccaca aattttaaca
     1681 ttgttattac aagtaatgga tgatggtaat ttgactgatg gtcaaggtaa tgtcatcaac
     1741 tttaaaaata caattattat ttgtacatca aatgctggct ttggcaatgg caatgacgct
     1801 gaagaaaaag atattatgca cgaaatgaaa aaattcttcc gccctgaatt ccttaaccgc
     1861 ttcaacggca tcgttgaatt cttacattta gataaagatg cattgcaaga tatcgtcaac
     1921 ttattattag acgatgtaca agttacatta gacaaaaaag gtattacgat ggacgtttct
     1981 caagatgcga aagattggtt aattgaagaa ggctatgatg aagaattagg tgcacgtcca
     2041 ttaagacgta ttgttgaaca gcaagtacgt gacaaaatta cagattacta tttagatcat
     2101 acagacgtta aacatgtgga tatagatgtt gaggataacg aattagtcgt aaaaggtaaa
     2161 taacgacact ttaacatatc gcgcatcaaa aatgagcatc aggtcgccct tgcctgtgct
     2221 cattttttta attatttccc tggaaaatga ttcgctgtgt gctgttctgt tccacaacaa
     2281 tcacgattaa tgtcacatgt accacatttt ccttgttttg aacgcttgaa aaattttact
     2341 agtgtatata aggcatatcc gaaaattgct aaaaaaatta aaatgttaat aatgactgac
     2401 actttaacca ctccttaaac aaataaatgt ccgacttgat aaaaaatgaa tgttaagaca
     2461 tatgcagtga ctagaggata ggcaactgca agtgccgtcc atttccatga ataagtctct
     2521 ttacggattg ctgctactgt agaaacacaa ggaatataca atagtataaa tatcataaat
     2581 gcatacgcag atagcggtgt gaattgattt tgaatcacat taacaaggcc tgcatcacct
     2641 gatgaataga taatcgccat cgaacttacg ataacttctt ttgctaaaaa tcctggcact
     2701 aacgtagcac ctgcttgcca tgttccaaat ccgagcggtt gcactaacat accaaagaaa
     2761 ctaccaacca tatgtaaaaa actttgattg atattcacat tgataccatg tggtcctaca
     2821 taacttaata gccaaatgac tactgagccg ccaaaaataa atgtacctgc tttacgaaca
     2881 aagcccttag ccttttccca agtactacgc cacaacgttt taatggaagg cacacggtat
     2941 gttggcaatt ccacaataaa gattgcatta tcatttttta aaatcgtctt agtaagtact
     3001 gtactgacta aaaatgccat aataatacct aaaacataca ggcttaatac tactaaagat
     3061 tgattctctt tgaaaaagat acctacgaac aacgcataca ctggcagtct agcagagcat
     3121 gacatgaatg gtgcaattaa tatcgttgtt aaacgctctt tttcattttc aatactgcgc
     3181 gcagccataa tactcggtac attacaacca aatccgataa tcattggtat aaaagacttc
     3241 ccgcttaaac cgaacgattc cataatacga tccattaaaa ccgcaatacg tgccatataa
     3301 cccgaatctt ctaataaaga tataaagaaa aagagcacaa caatttgtgg tacaaagact
     3361 aatactgatc ctacaccagc aataatgcca tctgtaatta aatcttgtaa aaatggtata
     3421 acaccaagat aattcataat cgtcttcaca ctatctgtaa atgtaccacc tataaatgca
     3481 tcgagttgat ccgacaaagg tgtgccaatc catgtaaatg tagtttgaaa gatcaaccac
     3541 ataattgcta gaaagatagg catccctata tatttatgtg ttaatatctt gtctattcta
     3601 gagctgaaat attgcttatc ttcatctgga tacgttacca cgtcttgcaa taacgtctca
     3661 atataatgat tgcgtatacg ctccatctct cgacgaacag atacagcccc tacttgttca
     3721 gcaacttgat cacgtaaact cgacaattta tttacaacct ctgaattaag ttcgtttgca
     3781 atttgcatgt tatttaataa gaattgaatc gcaataaacc tagcttgata cttatcatga
     3841 gatgtctctg tcattattat ttgacacata tttttaattg tctcttcaat cttctcacca
     3901 taattgattt taaaatgcgg ttgataccct tcccctagat gctttatttc gccaagtaaa
     3961 tattttgttc ctttgcctgt acgtgccaca actggaaaaa taggtgtttt taactttttc
     4021 atcaatttat gataatcgat ttttatcccg cgctttgtag ctacatcaat catatttaat
     4081 ccgatgtata ttggttgatt aagttctaac aattgtactg ttaattgcat atttcttttt
     4141 agttgactcg catcaacaat gttaatgatt cctgaaaatg aatcgtttaa taaatagtct
     4201 gtcactacag tttcatcttt agaaatcggc gataaatcat atgtacctgg taaatcaatt
     4261 aattgtccta cattttcttt aagtttccct acttttttct ctaccgttac gccactccag
     4321 ttgcctatat attcatacga accagttaaa gcgttaaaca aagatgtttt accaacatta
     4381 ggatttccta aaatacaata attttccatt cgtccggctc ctattcttct aatgcaatag
     4441 aacaagcatc gcaatgtcta atacttaact gttgtccgtt tacttcaata atacatggcc
     4501 ctttaaataa acatttttgt ttaatcgtta tgatagcgtc atctgttaac ccaaaggcac
     4561 ttagacgata caacatattc tcattagcaa tatccattcg ctttatttta taagccttat
     4621 tcatttcacc atttttaatg tttaacatac tatttcgctc tcctattaga aataataatc
     4681 attatcactt aaaaatcata acccttaaaa ttgtagctcg caatacttta tttaaataat
     4741 tttcattttt catgtaaaat ttgtgacatt gcaaaaatgt tttgtataat cacttccatg
     4801 caaaaaacct ccgcttcaaa tgtcattgat agacatttaa agcggaggca tgtacatgtt
     4861 attttatgat ttatttcgta gatttttttc taacaattga ttgagctttt caatattttt
     4921 attcaactgt tctatattcg ttgcttgttg ttttgaattt agttgatgct tgttttgata
     4981 atctattaaa gcatcatata agaacaatga acttgctgat tgctgtttca cttcttggta
     5041 caatgccatc gttttatcat ctagtactat tgaatgtgca tcatcttgcc catgtgcatc
     5101 ttgatgtttc gatacatttg ttgcaccttg tggatatact ttaaaggttg tgtcgtatgt
     5161 ttccttacta tctttagctt cagattcctg tgattcaacc gttttatatt tttcaagtgc
     5221 atgtccttca atatcaactc gtggaataat gcgattcaac catgctggta aataccacga
     5281 accttttcca aacaatttcg ttaatgcagg aattaacatc attctgacta cgaaggcatc
     5341 aaagagtaca ccaaacgcta atgccatacc cattgattta atcatgacat cttcttggaa
     5401 tacaaacgca aagaagacac taaacataat taatgcagct gctacaataa caggaccgct
     5461 ttctttcaat cctactttga tagaataatc attatcccct gttttactat attcttcatg
     5521 aattcgcgac ataaggaaga cttcataatc catcgctaat ccaaataaga tacctatagt
     5581 aataaccggt aaaaatgcta gcattggtcc tgtcgtttca ataccaaaca gacctttcat
     5641 aaaaccatct tgcattacta atgttgtaaa tcctaatgtt gccattaatg acaagacgaa
     5701 tcctaaaact gcttttaatg gtattagaat tgaacggaag acaatcatta ataagaaaaa
     5761 tgctaataca acaatgactg aggcaaataa aggtatcgcc tcatttaact ttttagacat
     5821 atcaatatta atgacacttt gtcccgaaat ctccgttttg aacccatatt tatcttgtgc
     5881 atctttatga taatctcgta aatcatgcac taaatcattt gtactctctg cattaggccc
     5941 ttgcttaggt atcacgacca tcaaagcgta atcattatct ttactcattt gtggtggcgt
     6001 aacgatatct acatttttct tatctttaat atctttatat acagactgta aatcttgttg
     6061 taatccttgt ggatcatcct ttttatcttt cacatttatc aacatcggta tttggccatt
     6121 aaatccttca ccaaatttat ccgagataat atcgtaagct tttttctgtg tagaatctgc
     6181 tggtttaaca ccgtcatctg gaataccaag tcgcatatga ctaactggta ttgcagctgc
     6241 tactaatatg attaaaccta gtaatactgc cgcaagtgca tttcctgtaa taaatttaga
     6301 ccatggcgta tcaatatctt ttttgaattt agactgtaat ttattcactt taatgcgttt
     6361 atggaaaatg cttattaatg caggtaataa agttaaagcg ctaagtactg caaaaacaac
     6421 actaattgcc gaagcaaatc ccattaccgc taagaagtca atgcctacta atgataaacc
     6481 acatactgca attacaactg ttacaccagc aaaaacaact gcactacctg ctgttcctat
     6541 tgcaagacca atgcctttaa tgtaatctgt ttcagttttc ataacttgtc gatatctgaa
     6601 taaaataaat aatgcataat cgataccaac tgctagtcca atcattacgg ctaatgtcag
     6661 tgtgacattt ggtatatcga atgcataagt taacaaactg ataataccta caccagaggc
     6721 tagaccaatc aatgcactta taattggtaa tcctgcagca atgactgaac cgaatgtgat
     6781 taacagtaca acaaatgcaa caataatacc aactagttca gaattaccgc ctacttctgt
     6841 agatgtcatt cctgtaccag ttaactctgt ttgtacatta tgattgtcac gtaagtcttt
     6901 caaatgactt ttaacattat ctctagagcc atcttttaaa gatgtttgac taacgtcata
     6961 tgtgatatct gcaaatgcag ttgttttatc tttactaatt tgcttatttt cataaggatc
     7021 tgatatttta tcaatgtgct tgtcatcttt tttaatatca tctaacgttt tcttaatatc
     7081 tttagtaatg ttcggttgca caataccatc atctttagtc gtcttaaaga caacacgtat
     7141 ttgtgccttt tcactatctt gattaaaatg tttttcaatc tttttattcg tatctaacga
     7201 ctctaatcct gtcattttaa tatcattgtc aaatttcggt gcatttgtag caagtggtat
     7261 caatattgca gctacaatca ctatccatgc aatgaccgcg gaccatttat gttttgcgat
     7321 gaatgtcccc atcttatata aaaattttgc caaagtatat tgcctccttt taaaatcaac
     7381 gttatagttt aaatatacag tgtagattat tgttcgatta tagtatctat ccccgacctc
     7441 ttaaagaatc aattggaaaa ttttgtatat taaactacac acaaaggaga aatgtagatg
     7501 aaagagactg atttacgagt tataaagaca aaaaaagcat tgtcgagtag cttgctacaa
     7561 ttgttagaac agcaattatt ccaaacgatt actgtcaatc aaatttgcga caacgcactc
     7621 gtacaccgta caacatttta taaacatttt tatgataaat atgatcttct agagtacttg
     7681 ttcaatcaat tgactaaaga ctactttgct agagatatca gtgaccgtct taatcatcca
     7741 ttccaaacga tgagtgatac gattaataat aaagaggatt tgagagaaat cgcagaattc
     7801 caagaagaag acgctgaatt taataaagta ttaaaaaatg tctgcattaa aattatgcat
     7861 aacgatatca aaaataatag agaccgtatc gatattgaca gcgacatccc agataatctc
     7921 atattttata tttatgactc gttgattgaa ggttttatac attggataaa agatgaaaaa
     7981 attgattggc ctggcgaaga tattgataac attttccata gattaatcaa tattaagatt
     8041 aaatagtaga tgagaaactc atgagcgtta ccaacattca taataaaaac gatagtgtac
     8101 acgttaatga attcgtgtac tactatcgtt ttttattttt atcgtgctta tcgctattaa
     8161 aacaactgat acacaacaca taaactatga agaaaaaaat aaatccgcta tctaaatgac
     8221 tttgactcag ttgtttaaat gaccaaattg ctaatacaat tcccattatt attgaaataa
     8281 cgtatctcac attcttatac ctataatcct tttctaaaaa tatggttgct attacttaat
     8341 ttttaaagtt ataaataaaa agagccaacc gcaatggatg gcccttgttc attatgaagc
     8401 attagaacat ttctgaaaca accttttgtt ctaagaagtg taataagtag tctggactac
     8461 ctgttttagc gtccgtacct gacattttga aaccaccaaa tggatggtat ccaacaactg
     8521 ctgaagtaca gcctctgtta aggtataaat tgcctacatc aaattcgttt accgctttaa
     8581 tccaatgctc gcgattattt gtaatcactg caccagttaa accgtaatct gtatcatttg
     8641 caacctcaat tgcttcatca aaatcgttaa ctttcacaaa gccaacaact ggaccaaaaa
     8701 tttcttcttg catgattcta tctttagatt taagtcctga aatgattgtt ggttctacaa
     8761 agtaaccttt tgaatcatca gtgccgccac cttgttctaa tttaccttct tctttaccaa
     8821 tctcaatata atttttaatc ttatcaaatt gttttttatt aataactggg cccatatacg
     8881 tattgtctac agtattgccc aacgttaatt cttttgttaa tttgattgat ttctctaata
     8941 cttcgtcata aacgtcttta tgcacaattg cacgtgaaca tgctgaacat ttttgaccag
     9001 aaaaaccaaa tgctgacgtt acaatagctt ctgctgccat atctgtatca atattttcat
     9061 caactacaat ggcatcttta ccacccattt cagcgataac acgtttcaag aagttttgac
     9121 cttcttgaac aacggcacta cgttcataaa ttctagtacc tgtcgcacgt gatcctgtaa
     9181 atgtaacgaa atgcgtatct ttatgatcaa ctaagtaatc accaatttct ttcggatcac
     9241 caggaacaaa gttaactacg ccttttggta atcctgcttc ttctaaaatt tccattaatt
     9301 tataagcgat ataaggtgta tcctcagcag gtttcaataa cactgtatta cctgccacaa
     9361 ctggtgctaa agttgtacca gccataatcg caaacgggaa gttccacggc ggaattgtaa
     9421 cacctgtacc aattgattta tagaaatatt tattgtgttc accttcacga tcaagtactg
     9481 gcttaccttg agccaagtcc atcattgaac gtgcatagta ttcaataaaa tcaatacctt
     9541 cagctgcatc accaactgct tcatcccatg gcttacctgc ttcataaacc ataattgctg
     9601 caatttccgc ttttcgacga cgaataattg ccgagacacg taacataagc tctgcacgat
     9661 catttgctga ccatgttttc caagatttat aagcttcgtt tgctgcttta aacgcatctt
     9721 caacatcttg ttttgttgcc tttgatgcat ttgcaatcac ttgtgatgtg tctgcaggat
     9781 tgattgattt aattttgtca tctttgaaaa tcttctctcc attaatcact aatggtatgt
     9841 cttgacctaa ttctttttcc acgtctttca atgctttctt aaacatatcc acattttctt
     9901 ggactgaaaa atcgtaacca ggttcatttt taaattctac taccatgtac acttaccccc
     9961 tataaatttt gaaagtggtt taaccctttg atttaatgat ataacatcat ttaaactcat
    10021 tttactatga ttaaggttag ttttgcaatc gctttcattt ttatgtttta tcacttattc
    10081 tcaagtattt tgaaattgat tggttacttt ttaaaattta tatgggtcgc aactgctact
    10141 ttatcgtttc gtcatttaat gtttcggatg gtaggtcatt atcaatttta cgaacgactt
    10201 tacaagggtt tccaaccgct aagctgtgtg gcggaatatc tttagtgaca acactaccag
    10261 caccaatcac actgccttct ccaatcgtca cccctggtaa cacggctaca tgaccgccaa
    10321 accaagtatt actgccaata tgaatgggtc cggctttttc aaaaccttca tttctatgat
    10381 ggaaattaag tggatgtgtc gctgtgtaga atccacaatt aggtcctata aaaacattat
    10441 cgccaattgt aatctgtcca ccatccataa agtaacaatt ggtgtttaca tagacatttt
    10501 ttcctagttt aacgttccaa ccataatctg tatcaaaagg aatcgaaata cttacattgt
    10561 ctgttgttgt ttgaaataat tgatcaatta attcctttct tttatttgta gcactcggtc
    10621 ttgtatgatt taattcaaag caaatatctt tcgctcgtgc acgttcattg attaagtatt
    10681 gatcaaagtt tgcatcgtac catttttctg ctaacatttt ttctttttca gtcattacac
    10741 ctttcaactc ctaataactt atttacttgt ttaaaagtta atcaaataaa ccttcgccta
    10801 tgcaactaat acgctataac attatgaaat catgacctta tcacccttat ctatacaatt
    10861 ctcgcatcaa atactgctaa agtagtagat aaattcaata ctacagacgc attcattttt
    10921 taatctatta acgtacaatg tgagtaagag aaatataaag gagtatgata gcgatgagaa
    10981 tattaattac aggcacagtt gctatcttaa tcattctagg tttggtcaaa acgatacaag
    11041 attacgaaat gacaaacgac acgagtcgtc agttgtcaga caacaaagat gatgataaag
    11101 tcatccatct taataatttt aaaaatttac atgcgaaaga atttaaccca tctgatttct
    11161 tttaagtcac ctaagaattg caaatccaga agtcatttaa gttttacctt tcattcatac
    11221 atcctttaat attaattacg acttctttta tatagatgct aagtagagag attgttgtgc
    11281 aatgtttgca cggcaatctc tctttttctt tttaaaattg gtaaaagtaa aacgcaacga
    11341 ttgacttata tacctatagg gggtacatta gacgtgtaac aatgaatcac agggaggcaa
    11401 taatgtggct aatacgaaaa aaacaacatt agatatcact ggtatgactt gtgccgcatg
    11461 ttcaaatcgt atcgaaaaga aactgaataa acttgatgac gttaatgccc aagtgaattt
    11521 aactacagag aaagcaactg ttgagtataa ccctgatcaa catgatgtcc aagaatttat
    11581 taatacgatt caacatttag gttacggtgt cgctgtagaa actgtcgaat tagacattac
    11641 aggtatgact tgtgctgcat gctcaagccg tattgaaaaa gtgttaaata aaatggacgg
    11701 cgttcaaaat gcaacggtca atttaacaac agagcaagct aaagttgact attatcctga
    11761 agaaacagat gctgataaac ttgtcactcg cattcaaaaa ttaggttatg acgcgtctat
    11821 taaagataac aataaagatc aaacgtcacg caaagctgaa gcgctacaac ataaattgat
    11881 taagcttatc atatcagcag tattatcttt accactatta atgttaatgt ttgtacatct
    11941 tttcaatatg catataccag cactatttac gaatccatgg ttccaattta ttttagctac
    12001 acctgtacaa tttattattg gatggcaatt ttatgtaggt gcttataaaa acttaagaaa
    12061 tggtggcgcc aatatggatg tacttgttgc tgttggtaca agtgcagcat atttttacag
    12121 tatttatgaa atggttcgtt ggctaaatgg ctcaacaacg caaccgcatt tatactttga
    12181 aacaagcgcc gtactaatta ccttaatctt attcggtaag tatttagaag ctagagcgaa
    12241 gtctcaaaca accaatgcgc ttggcgaatt attaagttta caagctaaag aagcacgcat
    12301 tttaaaagat ggtaatgaag tgatgattcc tctaaatgaa gtacatgttg gagatacact
    12361 tatcgttaaa ccaggtgaaa agatacctgt tgatggcaaa attattaaag gtatgactgc
    12421 catcgacgaa tctatgttaa caggtgaatc tatccctgtt gagaagaatg ttgatgatac
    12481 tgtaattggt tcaacgatga acaaaaacgg tactattact atgacagcaa caaaagttgg
    12541 cggggacact gcgttggcaa atattattaa agttgtcgaa gaagctcaaa gttctaaagc
    12601 gccgattcaa cgattggcag atattatttc tggttatttc gttcctatcg ttgttggtat
    12661 cgcactatta acatttatcg tgtggattac tttagttaca ccaggtacat ttgaacctgc
    12721 acttgttgcg agtatttccg ttctcgtcat tgcttgtcca tgcgcattgg gacttgctac
    12781 accaacttct attatggtag gtactggtcg cgctgctgaa aatggtattt tatttaaagg
    12841 tggcgagttt gttgaacgca cacatcaaat tgataccatc gttttagata agacgggtac
    12901 cattacaaat ggtcgtccag tcgtgacaga ttatcatggt gacaatcaaa cgctacaact
    12961 acttgctact gctgaaaaag attctgaaca cccattggca gaagccattg tcaattatgc
    13021 aaaagaaaag caattaatat taactgagac aacaacattt aaagcagtac ctggccatgg
    13081 tattgaagca acgattgatc atcactatat attggttggt aaccgtaaat taatggctga
    13141 caatgatatt agcttgccta agcatatttc tgatgattta acacattatg aacgagatgg
    13201 taaaactgct atgctcattg ctgttaatta ttcattaact ggtatcatcg cagtggcaga
    13261 tactgtcaaa gatcatgcca aagatgctat aaaacaattg catgatatgg gcattgaagt
    13321 tgccatgtta actggcgata ataaaaacac tgctcaagcc attgcaaaac aagtaggcat
    13381 agatactgtt attgcagata ttttaccaga agaaaaagct gcacaaattg cgaaactaca
    13441 gcaacaaggt aagaaggttg cgatggttgg tgacggtgta aatgatgcac ctgcattagt
    13501 taaagctgat atcggtatcg ccattggtac aggtacagaa gttgccattg aagcagctga
    13561 tattactatt cttggtggcg acttgatgct tattcctaaa gccatttatg caagtaaagc
    13621 aaccattcgt aatattcgtc aaaatctatt ttgggcattc ggctataata ttgccggtat
    13681 ccctatagct gcattgggct tacttgcgcc atgggttgct ggtgctgcaa tggcactaag
    13741 ttcagtaagt gttgtcacaa acgcacttag attgaaaaag atgcgattag aaccacgccg
    13801 taaagatgcc tagattcctt aataatgaag gattcgttgg tgattctgag ataggctagt
    13861 gattggctct ataatgtc
//
LOCUS       DAKTIP010000054        13874 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAKTIP010000054 DAKTIP010000000
VERSION     DAKTIP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.054"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            347..1138
                     /locus_tag="O0305_001218"
     CDS             347..1138
                     /locus_tag="O0305_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCY0274367.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            1153..1797
                     /gene="trmB"
                     /locus_tag="O0305_001219"
     CDS             1153..1797
                     /gene="trmB"
                     /locus_tag="O0305_001219"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCY0274368.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
     gene            2267..3109
                     /locus_tag="O0305_001220"
     CDS             2267..3109
                     /locus_tag="O0305_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0274369.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(3234..3545)
                     /locus_tag="O0305_001221"
     CDS             complement(3234..3545)
                     /locus_tag="O0305_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0274370.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            3632..4708
                     /locus_tag="O0305_001222"
     CDS             3632..4708
                     /locus_tag="O0305_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCY0274371.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            4773..5084
                     /locus_tag="O0305_001223"
     CDS             4773..5084
                     /locus_tag="O0305_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0274372.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            5184..6041
                     /locus_tag="O0305_001224"
     CDS             5184..6041
                     /locus_tag="O0305_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCY0274373.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            6070..6666
                     /locus_tag="O0305_001225"
     CDS             6070..6666
                     /locus_tag="O0305_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCY0274374.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            6687..10511
                     /locus_tag="O0305_001226"
     CDS             6687..10511
                     /locus_tag="O0305_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0274375.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEKRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            10535..11848
                     /locus_tag="O0305_001227"
     CDS             10535..11848
                     /locus_tag="O0305_001227"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCY0274376.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVILFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            11922..12413
                     /locus_tag="O0305_001228"
     CDS             11922..12413
                     /locus_tag="O0305_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCY0274377.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            12487..13845
                     /locus_tag="O0305_001229"
     CDS             12487..13845
                     /locus_tag="O0305_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274378.1"
                     /translation="MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNKESKAVALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEAS
                     ANNLSDISQEAQEVQEAKKEAQAEKDSDTLTKDASAAKVEVSKPESQAERLANAAKQK
                     QAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKAKSKIEKRTF
                     ND"
BASE COUNT         5374 a         1892 c         2595 g         4013 t
ORIGIN      
        1 catattttac taagtacgta aatgttacag ttcataaatt tatgctaacc atgaaaatgg
       61 gatagcaatt attttgaaaa ctagagttta tcgcctacag agattcaaaa acatgtagtt
      121 ttagacacgg taacattata tgtcaagtaa gtgactatag aatgtatggt gtcaatgaca
      181 gacattacga tttaaattta gtcgtgtaat gtaggcattg gttgaggatt tacaataatt
      241 atctggacat aatatttgta ccagtatggt gttataaatt agaatatatg gctcttgaaa
      301 aacctatttc tagagccatt ttctaattga aagtgaggtg gacgagttgg agcagtttta
      361 tcaattaggg tggacacttg attcagcagg tggtgcatct ggtgaagcat atatggctga
      421 acaagatgga caaaagttgt ttttaaaacg aaattcaaat ccatttattg cggcattatc
      481 agcagaaggt attgtgccca aattagtatg gacgaaacgc atagaaacag gcgaggttgt
      541 tacagcacaa cattggaaaa atgggcgtga actatcttca aacgaaatga agcaaacaag
      601 agttgcacat ttattaaaga agatacacaa ttctagacct ttattaagta tgttaaagcg
      661 tatggaaatg gaacctatta ctcctgagat tatgcttaat aaaattaatg cctctttatc
      721 aagagaagtt ttaacacatc atattgtgag aaaatcatta acctatttag aagagcatat
      781 accgagttta gattcgcgtt ttttcactgt tgtacatggt gatgtgaatc ataataattg
      841 gttattatcc gatcgagatg aactattttt agttgattgg gaaggtgcaa tgattgcaga
      901 cccggcaatt gatatcggaa tgctgcttta caactatgtt ccacaacaac aatggtctga
      961 atggcttgaa acatatggtg ttcaagagag cttgaattta aataaacgta tgaaatggta
     1021 tacggtaatt caatctatag gtcttgttca atggtatgaa gagcaaaaaa gatataagga
     1081 tatgaacact tggcttaaat tcctaaatga agttatgaat agtaacatgt ttatttaaga
     1141 ggagaataaa atatgagagt tcgatacaaa ccatgggctg aagattattt aaaagatcat
     1201 cctgaattgg ttgacatgga cggtcaacat gcagggaaaa tgactgaatg gtttgataaa
     1261 acgcaaccga tacacattga aattggttcg ggtatggggc agtttattac aacattagct
     1321 gctcaaaatc ctcatattaa ctatatttca atggaacgtg agaaaagtat cgtttataaa
     1381 gtattggata aagttaaaga gatgggttta acaaatttaa agattatttg taatgacgcc
     1441 attgaattaa atgaatattt taaagacggc gaagtttcgc gtatttattt gaacttttca
     1501 gatccatggc caaaaaatcg tcatgcaaaa cgacgtttaa cgtatcatac gtttttagcg
     1561 ttgtatcaac aaatcttaaa tgatgaagga gatttacatt ttaaaactga taacagagga
     1621 ctatttgcct acagtttaga aagtatgtct caatttggaa tgtattttac aaaaataaat
     1681 ctaaatttac atcaagaaga cgatggcagt aatattttaa cagaatatga aaagaaattt
     1741 tcggataaag ggtcacgtat ttatcgtatg gaagcgaaat tccattcaca aaaatagagc
     1801 acttatgagg gtatgtacac atacaatcat aagtgttttt tcgtactata atggttctat
     1861 agataaaggt tctataataa atcggactga tggaaaaagt ttttaacttt tcatctgtcc
     1921 gattttttga ttttgaatat aaaaaaagcg caattatctc tataattaaa agtgactaaa
     1981 caaataacta aaggagataa tgcgcctatg aacaatgtta tactaaatac ctttgattta
     2041 aaagaataaa gcttgcattt tacttgtaaa ttttaattat taatttaaaa atacaggccg
     2101 atggttatga tgcatttaca tataatatga ttgcattaat ctttataagt agttagaagt
     2161 gtattagtgt ggggcgtttc gtttttgttc gcatttatga atagtgatat gatgtaataa
     2221 acacatgcta attgtaataa cgaatacaga aagaataggt gatgttatga aaatcgggga
     2281 tatatctatt cattatctaa atggtggcaa tacaaaaatg gatggcggtg caatgtttgg
     2341 tgttgttccg aagccgttgt ggtcaaagca atacaatgca aatgaacgaa atcaaatcaa
     2401 tttaccgaca catccaattt tgattcaaac ggctcaatat aatttgatta tagatgcagg
     2461 tattggtaat ggtaaattat ctgaaaagca attacgtaat tttggagtag atgaagaaag
     2521 tcatataatt gctgatttgg caaattataa tttaacgcca aaggatattg attatgtgct
     2581 aatgacacat atgcattttg atcatgctgc cggtttgact gatcaagcag gacatgcaat
     2641 ttttgaaaat gcgattcatg ttgtgcaaca agatgagtgg catgagttta ttgcacctaa
     2701 tataagaagt aaatcaactt actgggataa gaataaaggc gattatagta acaagttgat
     2761 tttattcgaa aaacattttg aaccggttct gggtatcaag atgcaacata gtggaggtca
     2821 tagctttggc cacacgatta ttacgattga aagtcaagga gataaagcag ttcatatggg
     2881 tgatatattc ccaactactg cacataaaaa tcctctatgg gtaacggcat atgatgatta
     2941 tcctatgcaa tcgattcgtg aaaaagaacg catgatacca tattttattc agcaacaata
     3001 ttggttcttg ttttatcatg atgaaaacta ctttgctgta aaatacagcg ataatggtga
     3061 aaacatagat gcatatattt tacgtgaaac attagttgat aataactaaa ataaagatgt
     3121 attactaaac aaattttcaa aaataaaaaa ttgagccaca tccaatctta ctaattaggg
     3181 tgtggctcat ttttaagttt tacgatccaa atcaaatatg gataaaattc gtattaacgc
     3241 tctacaatgt taatgacttc accagtatat gcatctgcat aaaaatcata atgaatattt
     3301 tgaccatttt taatagttgt aattccacct tgataaacta aacggtattt atcagtttca
     3361 ggatgaacga atggttcata aacgatatat gagcctttga cattcataaa atatgatttt
     3421 acttcattaa gtactttaac gggattatag cgtttacgat taataaattg gatgattgaa
     3481 atggtagaaa taattacaat ggctactgca attattgttg gaattatata tttcagttta
     3541 gtcatcattt atgctccctt gatttcaaat tcatatcatt agtttaccat attgaagatg
     3601 atataataac ttataaggag tgagaacttt tatgaacata aataaaaaag taacattaca
     3661 acgaattcaa actttaactg agcttcatgg ggcaccaggt tttgaagaag aagtaaaaaa
     3721 ttatatgact cagcaaatgg cgccgtacgt agatgaattt attgaaaatc gtatgggtgg
     3781 attttttggt gtgaaaaaat ctaaaaatcc aaatgcaaaa cgtgtaatga ttgcagcaca
     3841 tatggatgaa atcggattta tgattacaaa tatcactaaa aatggaatga ttcaattcac
     3901 aaatttaggt ggtgttgcaa atgatatttg gcaaggacaa cgcttagtaa ttaaaaatag
     3961 aaatggcgat aaaattatcg gtgttgtttc taatatacct aaacattttc gtactggtag
     4021 tgaaggtgca ccggaaatta aagatttaac attagatata ggtgctcaaa atgaagatga
     4081 ggtgcgtgag cgcggaatag atataggaga tacaattgta cctcacacgc cattcacaca
     4141 gttatctgaa catcgatata gtgctaaagc atgggataat cgttatggtt gtgtcttggc
     4201 aattgaaata ctagaattat taaaagatat agaattagat gtagacttgt atgttggcgc
     4261 aaatgttcaa gaagaggttg gattacgagg tgcgaaagca tctgcagaga tgatagaccc
     4321 agacgttgca tttgtagttg attgttcacc tgccaatgac gttaaaggaa accaaccatt
     4381 atctggtgaa cttggtaaag ggacgttaat tcgcataaaa gacggtacaa tgattttaaa
     4441 gcctgtattt agagactatt tattaaagtt agtagaagca catgacattg aacatcaata
     4501 ctatatgtca ccaggtggaa cagatggtgg agaaattcat aaagctaata ttggtattcc
     4561 gactgcagtt attggtgtat gtgcacgata tattcatagt acagactcag tatttgatat
     4621 aagagactat tttgcagcta gatctttact ttcagaagcc atttgtaatt tagataataa
     4681 tcaaatagaa acattacaat ataaataatc gggtaataac aactattatc tctaaatagt
     4741 tatatataat cattaattaa ggagacataa aaatgaaaca acttgaatca gaacaacaat
     4801 ttgaatcttt aaaacaaggt gctacagtat ttgaattcac tgcaggctgg tgtccagatt
     4861 gtagagtgat agaaccagat ttaccggaat tagaagcgag atatcctatg tttgacttcg
     4921 tatcagtaga ccgtgataaa tttatggata tttgtattga aaatggtatt atgggtattc
     4981 caagttttct agtatataaa aatggagaac tgcttggaag ttatattgga aaagaacgaa
     5041 aatcaattga acagatagat gcatttttag ctcaatacgt gtaatttaga ctagagaaaa
     5101 acggggtaat actcgttttt ctctgttact atgtgttgat ttattgtaaa ctattataag
     5161 gtgcgaaatt aggagtgtta catatgaata cctttcaaat gagagataaa ttaaaggaac
     5221 gtttaagcca tttagacgtt gattttaaat ttaatcgtga agaagaaact ttgcgtattt
     5281 atcgaacaga taataacaaa ggtatcacga ttaaacttaa cgctatagtc gcaaaatatg
     5341 aagataaaaa agaaaaaatt gtagatgaaa ttgtttatta cgttgatgaa gctattgcac
     5401 aaatggcaga taaaaccctt gagagtatat catctagtca aattatgcct gtcattagag
     5461 cgactagctt tgataaaaaa actaaacaag gtgttccttt tatctatgat gagcatactg
     5521 cagaaacagc agtttattat gcagtcgatt tagggaaatc atatcgtcta attgacgaaa
     5581 gcatgttaga ggatttgaag ttaactgaac aacaaataag agaaatgtct ctgtttaatg
     5641 ttagaaaatt gtcaaattca tatacgactg atgaagtaaa aggtaatatt ttttatttta
     5701 ttaactcaaa tgacgggtat gatgcaagta ggatactaaa tactgcattt ttaaatgaaa
     5761 ttgaggcaca atgtcaaggc gaaatgctcg tagcagtgcc acaccaagat gtgttaatta
     5821 ttgcagatat acgcaataaa acaggatatg atgtgatggc acatttaaca atggaatttt
     5881 tcactaaagg tctagttcca attacatcat tatcctttgg atataaacag ggtcatcttg
     5941 aaccgatatt tattttaggt aaaaataata aacaaaaaag agatccaaac gtgattcagc
     6001 gtttagaagc aaatcgtcgt aaatttaata aagataaata gaaataattg gataaggagt
     6061 tttgtcataa tgaatttatt ttacaatcct aaatatgtag gagatgtcgc atttttacaa
     6121 attgaaccag ttgaaggtga attaaactac aataaaaaag gtaatgttgt tgaaattact
     6181 aatgaaggta atgttgtagg ttataatatt tttgaaattt caaaagatat aacaattgaa
     6241 gaaaaaggtc atattaaatt aactgatgaa cttgtaaatg tattccaaaa gcgtatttca
     6301 gaagctggtt ttgattataa attaaatgct gatctatcac cgaaatttgt agttggctac
     6361 gttgaaacta aagacaaaca tcctgatgca gataaattaa gtgtactaaa tgtaaacgtt
     6421 ggaaatgaca cattacaaat tgtatgtggc gcgcctaacg ttgaagctgg acagaaagtt
     6481 gttgttgcta aagtaggtgc agtgatgcct agcggtatgg taattaaaga tgctgaatta
     6541 cgtggtgttg cctcaagcgg tatgatttgt tcaatgaaag aattgaattt acctaatgca
     6601 cctgaagaaa aaggtattat ggtattaaat gacagctatg aaattggaca agcatttttt
     6661 gaataattaa ggaaggtagt gaaaatatga gctggtttga taaattattc ggcgaagata
     6721 atgattcaaa tgatgacttg attcatagaa agaaaaaaag acgtcaagaa tcacaaaata
     6781 tagataacga tcatgactca ttactgcctc aaaataatga tatttatagt cgtccgaggg
     6841 gaaaattccg ttttcctatg agcgtagctt atgaaaatga aaatgttgaa caatctgcag
     6901 atactatttc agatgaaaaa gaacaatacc atcgagacta tcgcaaacaa agccacgatt
     6961 ctcgttcaca aaaacgacat cgccgtagaa gaaatcaaac aactgaagaa caaaattata
     7021 gtgaaaaacg tgggaattct aaaatatcac agcaaagtat aaaatataaa gatcattcac
     7081 attaccatac gaataagcca ggtacatatg tttctgcaat taatggtatt gagaaggaaa
     7141 cgcacaagcc aaaaacacat aatatgtatt ctaataatac aaatcatcgt gctaaagatt
     7201 caactccaga ttatcacaaa gaaagtttca agacttcaga ggtaccgtca gctatttttg
     7261 gcacaatgaa acctaaaaag ttagaaaatg gtcgtatccc tgtaagtaaa ccttcagaaa
     7321 aagttgagtc agataaacaa aaatatgata aatatgtagc taagacgcaa acgtctcaaa
     7381 ataaacaatt agaacaagaa aaacaaaatg atagtgttgt caaacaagga actgcatcta
     7441 aatcatctga tgaaaatgta tcatcaacaa caaaatcaat gcctaattat tcaaaagttg
     7501 ataatactat caaaattgaa aatatttatg cttcacaaat tgttgaagaa attagacgtg
     7561 aacgagaacg taaagtgctt caaaagcgtc gatttaaaaa agcgttgcaa caaaagcgtg
     7621 aagaacataa aaacgaagag caagatgcaa tacaacgtgc aattgatgaa atgtatgcta
     7681 aacaagcgga acgctatgtt ggtgatagtt cattaaatga tgatagtgac ttaacagata
     7741 atagtacaga tgctagtcag cttcatacaa atggcataga gaatgaaact gtatcaaatg
     7801 atgaaaataa acaagcgtca atacaaaatg aagacactaa tgacactcat gtagatgaaa
     7861 gtccatacaa ttatgaggaa gttagtttga atcaagtatc gacaacaaaa caattgtcag
     7921 atgatgaagt tacggtttcg aatgtaacgt ctcaacatca atcagcacta caacataacg
     7981 ttgaagtaaa tgataaagat gaactaaaaa atcaatccag attaattgct gattcagaag
     8041 aagatggagc aacgaataaa gaagaatatt caggaagtca aatcgatgat gcagaatttt
     8101 atgaattaaa tgatacagaa gtagatgagg atactacttc aaatatcgaa gataatacca
     8161 atagaaacgc gtctgaaatg catgtagacg ctcctaaaac gcaagagtac gcagtaactg
     8221 aatctcaagt aaataatatc gataaaacgg ttgataatga aattgaatta gcaccgcgtc
     8281 ataaaaaaga tgaccaaaca aacttaagtg tcaactcatt gaaaacgaat gatgtgaatg
     8341 ataatcatgt tgtggaagat tcaagcatga atgaaataga aaagaataac gcagaaatta
     8401 cagaaaatgt gcaaaacgaa gcagctgaaa gtgaacaaaa tgtcgaagag aaaactattg
     8461 aaaacgtaaa tccaaagaaa cagactgaaa aggtttcaac tttaagtaaa agaccattta
     8521 atgttgtcat gacgccatct gataaaaagc gtatgatgga tcgtaaaaag cattcaaaag
     8581 tcaatgtgcc tgaattaaag cctgtacaaa gtaagcaagc tgtgagtgaa agaatgcctg
     8641 cgagtcaagc cacaccatca tcaagatctg attcacaaga gtcaaataca aatgcatata
     8701 aaacaaataa tatgacatca aacaatgttg agaacaatca acttattggt catgcagaaa
     8761 cagaaaatga ttatcaaaat gcacaacaat attcagagca gaaaccttct gttgattcaa
     8821 ctcaaacgga aatatttgaa gaaagtcaag atgataatca attggaaaat gagcaagttg
     8881 atcaatcaac ttcgtcttca gtttcagaag taagcgacat aactgaagaa agcgaagaaa
     8941 caacacatcc aaacaatact agtggacaac aagataatga tgatcaacaa aaagatttac
     9001 agtcatcatt ttcaaataaa aatgaagata cagctaatga aaatagacct cggacgaacc
     9061 aacaagatgt tgcaacaaat caagctgtac aaacatctaa gccgatgatt cgtaaaggcc
     9121 caaatattaa attgccaagt gtttcattac tagaagaacc acaagttatt gagtcggacg
     9181 aggactggat tacagataaa aagaaagaac tgaatgacgc attattttac tttaatgtac
     9241 ctgcagaagt acaagatgta actgaaggtc caagtgttac aagatttgaa ttatcagttg
     9301 aaaaaggtgt taaagtttca agaattacgg cattacaaga tgacattaaa atggcattgg
     9361 cagcgaaaga tattcgtata gaagcgccta ttccaggaac tagtcgtgtt ggtattgaag
     9421 ttccgaacca aaatccaacg acagtcaact tacgttctat tattgaatct ccaagtttta
     9481 aaaatgctga atctaaatta acagttgcga tggggtatag aattaataat gaaccattac
     9541 ttatggatat tgctaaaacg ccacacgcac taattgcagg tgcaactgga tcagggaaat
     9601 cagtttgtat caatagtatt ttgatgtctt tactatataa aaatcatcct gaggaattaa
     9661 gattattact tatcgatcca aaaatggttg aattagctcc ttataatggt ttgccacatt
     9721 tagttgcacc ggtaattaca gatgtcaaag cagctacaca gagtttaaaa tgggccgtag
     9781 aagaaatgga acgacgttat aagttatttg cacattacca tgtacgtaat ataacagcat
     9841 ttaacaaaaa agcaccatat gatgaaagaa tgccaaaaat tgtcattgta attgatgagt
     9901 tggctgattt aatgatgatg gctccgcaag aagttgaaca gtctattgct agaattgctc
     9961 aaaaagcgag agcatgtggt attcatatgt tagtagctac gcaaagacca tctgtcaatg
    10021 taattacagg tttaattaaa gccaacatac caacaagaat tgcatttatg gtatcatcaa
    10081 gtgtagattc gagaacgata ttagacagtg gtggagcaga acgcttgtta ggatatggcg
    10141 atatgttata tcttggtagc ggtatgaata aaccgattag agttcaaggt acatttgttt
    10201 ctgatgacga aattgatgat gttgttgatt ttatcaaaca acaaagagaa ccggactatc
    10261 tatttgaaga aaaagaattg ttgaaaaaaa cacaaacaca atcacaagat gaattatttg
    10321 atgatgtttg tgcatttatg gttaatgaag gacatatttc aacatcatta atccaaagac
    10381 atttccaaat tggctataat agagcagcaa gaattatcga tcaattagag caactcggtt
    10441 atgtttcgag tgctaatggt tcaaaaccaa gggatgttta tgttacggaa gcagatttaa
    10501 ataaagaata attatgagta aggagtttta tataatgaca cactatcatt ttgtcggaat
    10561 taaaggttct ggcatgagtt cattagcaca aatcatgcat gatttaggac atgaagttca
    10621 aggatcggat attgagaact acgtatttac agaagttgct cttagaaata aggggataaa
    10681 aatattacca tttgatgcta ataacataaa agaagatatg gtagttatac aaggtaatgc
    10741 attcgcgagt agccatgaag aaatagtacg tgcacatcaa ttgaaattag atgttgtaag
    10801 ttataatgat tttttaggac agattattga tcaatatact tcagtagctg taactggtgc
    10861 acatggtaaa acttctacaa caggtttatt atcacatgtt atgaatggtg ataaaaagac
    10921 ttcattttta attggtgatg gcacaggtat gggattgcct gaaagtgatt atttcgcttt
    10981 tgaggcatgt gaatatagac gtcacttttt aagttataaa cctgattacg caattatgac
    11041 aaatattgat ttcgatcatc ctgattattt taaagatatt aatgatgttt ttgatgcatt
    11101 ccaagaaatg gcacataatg ttaaaaaagg tattattgct tggggtgatg atgaacatct
    11161 acgtaaaatt gaagcagatg ttccaattta ttattatgga tttaaagatt cggatgacat
    11221 ttatgctcaa aatattcaaa ttacggataa aggtactgct tttgatgtgt atgtggatgg
    11281 tgagttttat gatcacttcc tgtctccaca atatggtgac catacagttt taaatgcatt
    11341 agctgtaatt gcgattagtt atttagagaa gctagatgtt acaaatatta aagaagcatt
    11401 agaaacgttt ggtggtgtta aacgtcgttt caatgaaact acaattgcaa atcaagttat
    11461 tgtagatgat tatgcacacc atccaagaga aattagtgct acaattgaaa cagcacgaaa
    11521 gaaatatcca cataaagaag ttgttgcagt atttcaacca cacactttct ctagaacaca
    11581 ggcattttta aatgaatttg cagaaagttt aagtaaagca gatcgtgtat tcttatgtga
    11641 aatttttgga tcaattagag aaaatactgg cgcattaacg atacaagatt taattgataa
    11701 aattgaaggt gcatcgttaa ttaatgaaga ttctattaat gtattagaac aatttgataa
    11761 tgctgttatt ttatttatgg gtgcaggtga tattcaaaaa ttacaaaatg catatttaga
    11821 taaattaggc atgaaaaatg cgttttaata tgtttataat agagtagtat gggtatttat
    11881 tattaatgac attattacat gttaattagg aggcgttttt aatggattgg attttaccaa
    11941 ttgctggaat tatcgctgcg attgcattct taattttatg tatcggtatc gtagctgtat
    12001 taaattctgt taagaaaaac ttagattatg ttgcaaaaac acttgacggt gtagaaggtc
    12061 aagttcaagg tattactcgt gaaacaacag atttacttca taaagtaaac cgtttaactg
    12121 aggatatcca aggtaaagta gatcgtttaa actcagttgt agatgctgtt aaaggtatcg
    12181 gtgactcagt acaaacgtta aacagctctg tagatcgtgt aacaaattca attacacata
    12241 atatttctca aaatgaagat aaaatctcac aagttgttca atggtcaaat gttgcaatgg
    12301 aaattgcaga caaatggcaa aatagacact accgtcgtgg aagtgcaaat tacaaagcta
    12361 ataatgtagc aactgatgca aatcatagct atacttctag agtagataaa taattaataa
    12421 ttaaacaaat gcacttaaat gaggttgtta cttatttaag tgcatttgtt ataggaggat
    12481 atacaaatga cgcagcaaca aaatgataaa agaacattaa aaaataaaca cacttatcaa
    12541 aatgaaccac taccaaaccg taaagatttt gttgttagtt ttataactgg cgcgcttgtt
    12601 ggttcagctt taggtttgta ttttaaaaat aaagtttatc aaaaagcaga tgatttaaaa
    12661 gtcaaagaac aagaactgtc gcaaaagttt gaagaaagaa aaacgcaact tgaagaaacg
    12721 gttgcctata caaaagaacg tgttgaagga tttttaaaca aatctaaaaa tgagcaagcg
    12781 gcattaaagg cacaacaagc agcgataaaa gaagaagcaa gtgcaaataa tttaagtgat
    12841 acatcacaag aagcacaaga gattcaagaa gctaaaagag aagcacaagc agaagcggat
    12901 aaaagtgtgg ctgtatcaaa taaagaatca aaagcagtgg cattgaaagc acaacaagca
    12961 gcgataaaag aagaagcaag tgcaaataat ttgagtgata catcacaaga ggcacaagag
    13021 attcaagaag ctaaaaaaga agcacaagca gaaacagata aaagtgcagc tgtatcaaat
    13081 gaagaaccaa aagcagtggc attgaaagca caacaagcag cgataaaaga agaagcaagt
    13141 gcaaataatt taagtgatat atcacaagag gcacaagagg ttcaagaagc taaaaaagaa
    13201 gcacaagcag agaaagacag tgacacatta actaaagatg caagtgcagc aaaggtagaa
    13261 gtatcaaaac cagagtcaca agctgaaaga ttagcaaacg ctgcaaaaca gaagcaagct
    13321 aaattaacac caggttcaaa agagagtcaa ttaactgaag cgttatttgc agaaaaacca
    13381 gttgctaaaa atgacttgaa agaaattcct caattagtta ctaaaaagaa tgatgtatca
    13441 gagacagaga cggttaatat agataataaa gacactgtta aacaaaaaga agctaaattt
    13501 gaaaatggtg ttattacacg taaagctgat gaaaaaacaa ctaataatac agctgttgac
    13561 aagaaatcag gtaaacaatc taaaaaaaca acaccttcaa ataaacgaaa tgcatcaaaa
    13621 gcatctacaa ataaaacttc aggtcagaaa aagcaacata ataagaaatc atcacaaggt
    13681 gcaaagaaac aaagtagttc aagtaagtca actcaaaaga ataatcaaac tagtaataag
    13741 aattcaaaaa caacaaatgc taaatcatcc aatgcatcaa aaacgccaaa tgctaaagtt
    13801 gagaaagcta aaagtaaaat agagaaacgt acattcaatg actaagatta ctaaaatcgt
    13861 tgaataatca atca
//
LOCUS       DAKTIP010000055        13714 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAKTIP010000055 DAKTIP010000000
VERSION     DAKTIP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.055"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            183..476
                     /gene="esxA"
                     /locus_tag="O0305_001230"
     CDS             183..476
                     /gene="esxA"
                     /locus_tag="O0305_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCY0274379.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            560..3589
                     /gene="esaA"
                     /locus_tag="O0305_001231"
     CDS             560..3589
                     /gene="esaA"
                     /locus_tag="O0305_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCY0274380.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLVLVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            3589..4047
                     /gene="essA"
                     /locus_tag="O0305_001232"
     CDS             3589..4047
                     /gene="essA"
                     /locus_tag="O0305_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCY0274381.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            4019..4261
                     /gene="esaB"
                     /locus_tag="O0305_001233"
     CDS             4019..4261
                     /gene="esaB"
                     /locus_tag="O0305_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCY0274382.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            4274..5608
                     /gene="essB"
                     /locus_tag="O0305_001234"
     CDS             4274..5608
                     /gene="essB"
                     /locus_tag="O0305_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCY0274383.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            5630..10069
                     /gene="essC"
                     /locus_tag="O0305_001235"
     CDS             5630..10069
                     /gene="essC"
                     /locus_tag="O0305_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCY0274384.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRIFNDEIDRRK
                     KILSQYRVTSISEYRKLTGETIPHVFILIDNFDAVKDSPFQEVFENMMIKMTREGLAL
                     DMQVTLTASRANAMKTPMYINMKTRIAMFLYDKSEVSNVVGQQKFAVKDVVGRALLSS
                     DDNVSFHIGQPFKHDETKSYNDQINDEVSAMTEFYKGETPNDIPMMPDEIKYEDYRES
                     LNLPDIVANGALPIGLDYEGVTLQKIKLTEPAMISSENPREIAHIAEIMMKEIDILNE
                     KYAICIADSSGEFKAYRHQVANFAEEREDIKAIHQLMIEDLKQREMDGPFEKDTLYII
                     NDFKTFIDCTYIPEDDVKKLITKGPELGLNILFVGIHKELIDAYDKQIDVARKMINQF
                     SIGIRISDQQFFKFRFIQREPVIKENEAYMVANQAYQKIRWFK"
     gene            10099..10491
                     /gene="esaC"
                     /locus_tag="O0305_001236"
     CDS             10099..10491
                     /gene="esaC"
                     /locus_tag="O0305_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion substrate EsaC"
                     /protein_id="HCY0274385.1"
                     /translation="MNFNDIETMVKSKFKDIKKHAEEIAHEIEVRSGYLRKAEQYKRL
                     EFNLSFALDDIESTAKDVQTAKSSANKDSVTVKGKAPNTLYIEKRNLMKQKLEMLGED
                     IDKNKESLQKAKEIAGEKASEYFNKAMN"
     gene            10507..10821
                     /gene="esxB"
                     /locus_tag="O0305_001237"
     CDS             10507..10821
                     /gene="esxB"
                     /locus_tag="O0305_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxB"
                     /protein_id="HCY0274386.1"
                     /translation="MGGYKGIKADGGKVDQAKQLAAKTAKDIEACQKQTQQLAEYIEG
                     SDWEGQFANKVKDVLLIMAKFQEELVQPMADHQKAIDNLSQNLAKYDTLSIKQGLDRV
                     NP"
     gene            10821..11495
                     /locus_tag="O0305_001238"
     CDS             10821..11495
                     /locus_tag="O0305_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5081 family protein"
                     /protein_id="HCY0274387.1"
                     /translation="MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALI
                     EKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNN
                     GFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLE
                     IEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENH
                     INKAEE"
     gene            11495..11812
                     /locus_tag="O0305_001239"
     CDS             11495..11812
                     /locus_tag="O0305_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274388.1"
                     /translation="MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYY
                     SSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYN
                     IEG"
     gene            11822..13666
                     /locus_tag="O0305_001240"
     CDS             11822..13666
                     /locus_tag="O0305_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease YeeF family protein"
                     /protein_id="HCY0274389.1"
                     /translation="MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSD
                     VSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKA
                     FKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVK
                     VIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALA
                     GMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGM
                     GFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQ
                     NTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGM
                     GGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVES
                     PNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRN
                     SHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKE
                     WQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK"
BASE COUNT         5357 a         1909 c         2592 g         3856 t
ORIGIN      
        1 aatggttaat taaaaatata tgggtatatt caatatattt atttaaaaaa agctaaaaat
       61 acttaaaaaa ctatatacat atactaataa tttatatatt atttgagtaa ggagcacttt
      121 ttcaaaaaat agtgtcccta aaaagttttg ataaacttaa aatattcagg aggtttctag
      181 ttatggcaat gattaagatg agtccagagg aaatcagagc aaaatcgcaa tcttacgggc
      241 aaggttcaga ccaaatccgt caaattttat ctgatttaac acgtgcacaa ggtgaaattg
      301 cagcgaactg ggaaggtcaa gctttcagcc gtttcgaaga gcaattccaa caacttagtc
      361 ctaaagtaga aaaatttgca caattattag aagaaattaa acaacaattg aatagcactg
      421 ctgatgccgt tcaagaacaa gaccaacaac tttctaataa tttcggtttg caataagcat
      481 tctgaaattg gcaaagtcac attttctaat gtggctttgc ttatcatttt tttaagaaaa
      541 caactgaaag gaaataagca tgaaaaagaa aaattggatt tatgcattaa ttgtcacttt
      601 aattattata attgccatag ttagtatgat attttttgtt caaacaaaat atggagatca
      661 atcagaaaaa ggatcccaaa gtgtaagtaa taaaaataat aaaatacata tcgcaattgt
      721 taacgaggat caaccaacga catataacgg taaaaaagtt gagctgggtc aagcatttat
      781 taaaaggtta gcaaatgaga aaaactataa atttgaaaca gtaacaagaa acgttgctga
      841 gtctggtttg aaaaatggtg gataccaagt catgattgtt atcccagaaa acttttcaaa
      901 attggcaatg caattagacg ctaaaacacc atcgaaaata tcgctacagt ataaaacagc
      961 tgtaggacaa aaagaagaag tagctaaaaa cacagaaaaa gttgtaagta atgtacttaa
     1021 cgactttaac aaaaacttag tcgaaattta tttaacaagc atcattgata atttacataa
     1081 tgcacaaaaa aatgttggcg ctattatgac gcgtgaacat ggtgtgaata gtaaattctc
     1141 gaattactta ttaaatccaa ttaacgactt cccggaatta tttacagata cgcttgtaaa
     1201 ttcaatttct gcaaacaaag acattacaaa atggttccaa acatacaata aatcattatt
     1261 gagtgcgaat tcagatacgt tcagagtgaa cacagattat aatgtttcga ctttaattga
     1321 aaaacaaaat tcattatttg acgagcacaa tacagcgatg gataaaatgt tacaagatta
     1381 taaatcgcaa aaagatagcg tggaacttga taactatatc aatgcattaa aacagatgga
     1441 cagccaaatt gatcaacaat caagtatgca agatacaggt aaagaagaat ataaacaaac
     1501 tgttaaagaa aacttagata aattaagaga aatcattcaa tcacaagagt caccattttc
     1561 aaaaggtatg attgaagact atcgtaagca attaacagaa tcactgcaag atgagcttgc
     1621 aaataacaaa gacttacaag atgcgctaaa tagcattaaa atgaacaatg ctcaattcgc
     1681 tgaaaactta gagaaacaac ttcatgatga tattgtcaaa gaacctgata cagatacaac
     1741 atttatctat aacatgtcta aacaagactt tatagctgca ggtttaaatg aggatgaagc
     1801 taataaatac gaagcaattg tcaaagaagc aaaacgttat aaaaatgaat ataatttgaa
     1861 aaaaccgtta gcagaacaca ttaatttaac agattacgat aaccaagttg cgcaagacac
     1921 aagtagtttg attaatgatg gtgtcaaagt gcaacgtact gaaacgatta aaagtaatga
     1981 tattaatcaa ttaactgttg caacagatcc tcattttaat tttgaaggcg acattaaaat
     2041 taatggtaaa aaatatgaca ttaaggatca aagtgttcaa ctcgatacat ctaacaagga
     2101 atataaagtt gaagtcaatg gcgttgctaa attgaaaaag gatgctgaga aagatttctt
     2161 aaaagataaa acaatgcatt tacaattgtt atttggacaa gcaaatcgtc aagatgaacc
     2221 aaatgataag aaagcaacga gtgttgtgga tgtaacattg aatcataacc ttgatggtcg
     2281 cttatcgaaa gatgcattaa gccagcaatt gagtgcatta tctaggtttg atgcgcatta
     2341 taaaatgtac acagatacaa aaggcagaga agataaacca ttcgacaaca aacgtttaat
     2401 tgatatgatg gttgaccaag ttatcaatga catggaaagt ttcaaagacg ataaagtagc
     2461 tgtgttacat caaattgatt caatggaaga aaactcagac aaactgattg atgacatttt
     2521 aaataacaaa aagaatacaa caaaaaataa agaagatatt tccaagctga ttgatcagtt
     2581 agaaaacgtt aaaaagactt ttgctgaaga gccacaagaa ccaaaaattg ataaaggcaa
     2641 aaatgatgaa tttaatacga tgtcttcaaa tttagataaa gaaattagta gaatttctga
     2701 aaagagtacg caattgctat cagatacaca agaatcaaaa tcaattgcag attctgttag
     2761 tggccaatta aatcaagtcg acaataatgt gaataagcta catgcgacag gtcgagcatt
     2821 aggcgtaaga gctaacgatt tgaatcgtca aatggctaaa aacgataaag ataatgagtt
     2881 gttcgctaaa gaatttaaaa aagtattaca aaattctaaa gatggcgaca gacaaaacca
     2941 agcattaaaa gcatttatga gtaatccggt tcaaaagaaa aacttagaaa atgttttagc
     3001 taataatggt aatacagacg tgatttcacc gacattattc gtattattga tgtatttact
     3061 atcaatgatt acagcatata ttttctatag ttatgaacgt gccaaaggac aaatgaattt
     3121 cattaaagat gattatagta gtaaaaacca tctttggaat aatgtcatta cgtcaggtgt
     3181 tattggtaca actggtttgg tagaagggtt aattgtcggt ttaattgcaa tgaataagtt
     3241 ccatgtatta gctggctata gagcgaaatt catcttaatg gtgattttaa ctatgatggt
     3301 cttcgtactt attaatacgt atttactaag acaggtaaaa tctatcggta tgttcttaat
     3361 gattgctgca ttgggtctat actttgtagc tatgaataat ttgaaagcag ctggacaagg
     3421 tgtgactaat aaaatttcac cattgtctta tatcgataac atgttcttca attatttaaa
     3481 tgcagagcat cctataggct tggtgctagt aatattaaca gtacttgtga ttattggctt
     3541 tgtactgaac atgtttataa aacactttaa gaaagagaga ttaatctaat gttgatgaat
     3601 agcgtgattg ctttaacttt tttaacagca tctagcaata atggcggact taatattgat
     3661 gtgcaacaag aagaggaaaa gcgaatcaat aatgatttaa atcaatatga tacaacgcta
     3721 tttaataaag acagcaaagc ggttaatgat gcgattgcta agcagaaaaa agaacgacaa
     3781 caacaaataa aaaatgatat gtttcaaaat caagcgagtc actcgactcg cttgaatgaa
     3841 actaaaaaag tgttattttc caaatctaac ttagaaaaga cttcggagag tgataaaagc
     3901 ccctatattc aaaacaagca ggagaaaaaa atattcccgt acattttgat gtctgtaggg
     3961 gcttttttga ctttaggatt tgtcattttt tcaattcata aagggagacg aacgaaaaat
     4021 gaatcagcac gtaaaagtaa catttgattt tactaattat aattacggca catatgactt
     4081 agcagtacca gcatatttac cgataaaaaa cttaatagct ttagtattgg atagtttgga
     4141 catttcaata tttgatgtca atacacaaat taaagtgatg acgaaaggtc aattacttgt
     4201 tgaaaatgat cgactcattg attatcaaat cgctgatgga gatattttga agttactata
     4261 ggaggaaaaa tagatggtta aaaatcataa ccctaaaaat gaaatgcaag atatgttaac
     4321 gcctttagat gctgaagaag cagctaaaac aaaattacgc ttagatatga gagagattcc
     4381 taagtcttca attaaaccag aacattttca tttaatgtac ttattagaac aacattctcc
     4441 atattttata gatgctgaat taactgaact acgtgacagt ttccaaatac attatgacat
     4501 taatgacaat catacacctt ttgataatat taaatcattt actaaaaatg aaaaattacg
     4561 ttacttactc aatatcaaaa atttagaaga agtaaatcgt acacgctaca catttgtgtt
     4621 ggcaccagat gaattatttt tcacaagaga tggattaccc attgctaaaa caagagggtt
     4681 acaaaatgtt gttgatccat tacctgtgtc agaagctgaa tttttaacaa gatataaagc
     4741 gctggttatc tgtgcattca atgagaaaca atcatttgat gctttagttg aaggaaactt
     4801 agaactacat aaaggaacgc catttgaaac taaagttatt gaagcggcaa cgttagattt
     4861 actaacggca tttttagatg aacagtatca gaaacaagaa caagattata gtcaaaatta
     4921 tgcatatgta cgcaaagtag gacataccgt tttcaaatgg gttgctatcg gtatgacaac
     4981 gttaagtgtt ttattaattg cattcttagc ctttttatat ttttcagtaa tgaagcataa
     5041 tgagcgcatt gaaaaaggat accaagcatt tgtaaaggat gattatacgc aagtactaaa
     5101 tacgtatgat gatttagatg gtaaaaaatt agataaagag gcactttaca tttatgccaa
     5161 aagttatatc caaacaaata aacaaggttt agaaaaagat aagaaagaaa atttacttaa
     5221 taatgtgaca ccaaattcaa acaaagacta cttattatat tggatggaat taggacaagg
     5281 acatcttgat gaagcgatta atattgccac ttatttagat gataacgata ttacaaagtt
     5341 agcgttgatt aataaattaa atgagattaa aaataacgga gatttatcga atgataaacg
     5401 ttctgaagaa acgaaaaagt ataacgataa attgcaagat attttagaca aagaaaaaca
     5461 agttaaagat gaaaaagcga aatctgaaga agagaaagca aaagcgaaag atgagaaatt
     5521 aaagcaacaa gaagagaacg aaaagaaaca aaaagaacaa gcacaaaaag ataaagaaaa
     5581 acgccaagaa gctgaaagaa aaaaatagta taggactgag gcaaagacaa tgcataaatt
     5641 gattataaaa tataacaaac aattgaagat gctcaatttg cgagatggta agacatatac
     5701 tattagcgaa gacgagcgtg cagatattac gttgaaatcg ttaggcgaag tcattcattt
     5761 agaacaaaat aatcaaggta cttggcaagc gaatcatact tctattaata aggtgcttgt
     5821 tagaaaaggt gaccttgatg acattacatt acagctttat acagaagctg attatgcatc
     5881 atttgcgtat ccttcaattc aagatacgat gacaattgga ccaaatgcgt atgatgatat
     5941 ggttattcaa agcttgatga atgccatcat tattaaagat tttcaatcaa tacaagaatc
     6001 acaatacgta cgcattgtgc acgataaaaa tacagatgtg tatattaact atgaactaca
     6061 agagcaacta acgaacaaag cttacattgg tgatcatatt tatgttgaag ggatatggct
     6121 cgaagtacaa gctgatggtt taaatgtatt gagtcagaat acagtggcat cgtcattaat
     6181 tcgcttaaca caagagatgc cacatgcaca ggcagatgat tacaatacgt accatcgttc
     6241 gccaaggatt attcaccgtg aaccgacgga tgatattaag attgaaagac cgccacagcc
     6301 aatacagaag aacaatacag tgatatggcg ttccattata ccgccattag taatgattgc
     6361 tttaactgtt gtcatctttt tagtgagacc aattggtatt tatattttaa tgatgattgg
     6421 tatgagtaca gtaacgatag tatttggtat tacaacgtat ttctctgaaa agaaaaagta
     6481 taacaaagat gttgaaaaac gagagaaaga ttacaaagct tatttggata ataaatctaa
     6541 agaaattaac aaagcgatta aagcacaacg ttttagtttg aattaccatt atccaacggt
     6601 tgctgaaatt aaagatatcg ttgaaacgaa agcaccaaga atatatgaaa aaacatcgca
     6661 tcatcacgat ttcttacatt ataagttagg tattgcgaat gtagaaaagt cattcaaatt
     6721 agattaccaa gaagaagaat ttaaccaacg tcgtgatgaa ctattcgacg atgctaaaga
     6781 attgtatgaa ttttacacag atgtagaaca agcaccatta atcaatgatt taaatcatgg
     6841 gccaattgca tatattggtg cacgacatct cattttagaa gaattggaga aaatgctaat
     6901 tcaattgtca acattccata gttatcatga tttagagttt ctatttgtga cacgtgaaga
     6961 tgaagttgaa acattgaaat gggcacgttg gttgccacat atgacattga gagggcaaaa
     7021 cattagagga tttgtttaca atcaacgaac gcgtgaccaa attttaacgt caatttatag
     7081 catgattaaa gaacgtatcc aagctgtgcg tgaacgcagc agaagtaatg agcaaattat
     7141 tttcacaccg caattagtgt ttgtcattac agatatgtca ttaattattg atcatgtcat
     7201 tttagaatat gtaaaccaag atttatcaga atatggtatt tcattaatct ttgttgaaga
     7261 tgtgattgaa agtttgccag agcatgtaga taccattatt gatatcaagt ctcgtactga
     7321 aggcgaactg attacgaaag aaaaagaatt agttcaattg aaatttacac ctgaaaatat
     7381 tgataacgtc gataaagaat atatcgcgcg acgtttggcg aatttgatac acgtcgaaca
     7441 tttgaaaaat gcaattcctg atagtattac atttttagag atgtataacg tgaaagaagt
     7501 agatcagctt gatgtggtta atcgatggag acaaaacgaa acatacaaaa cgatggcagt
     7561 acctttaggt gtaagaggta aagatgatat tttatcattg aacttacatg aaaaagcaca
     7621 cgggccacat ggtttagttg ctggtaccac tggttcaggg aaatctgaga ttatccaatc
     7681 atacatttta tctttagcta ttaattttca ccctcatgaa gttgcattcc tattgattga
     7741 ctataaaggt gggggtatgg cgaacttatt taaagattta gtccatttag ttggtacgat
     7801 tacaaactta gatggcgatg aagcgatgcg tgccttaaca tcaatcaaag ccgaattgag
     7861 aaaacgtcaa cgtttattcg gagagcatga tgttaaccat attaatcaat accataagtt
     7921 atttaaagaa ggtattgcga cagaaccaat gccacattta ttcattattt ccgatgagtt
     7981 tgccgaatta aaatcagaac aacctgattt tatgaaagaa cttgtatcaa cggcacgtat
     8041 tggacgttcg ttaggtattc atttaatact tgcgacacaa aaaccatcgg gtgttgttga
     8101 tgaccaaatt tggtctaact ctaaatttaa gttggcatta aaagtacaag atagacaaga
     8161 cagtaatgaa attttaaaaa caccagatgc agcagacatt acattaccag gtcgtgcgta
     8221 tttacaagtt ggtaataatg aaatttatga attattccaa tctgcatgga gtggtgcaac
     8281 atatgacatc gaaggcgata aattagaagt tgaagataag acgatttaca tgattaatga
     8341 ctatggtcaa cttcaagcaa tcaacaaaga cttgagtgga cttgaagatg aagaaacgaa
     8401 agaaaatcaa actgagttag aagcggtcat agatcatatc gaatctatta caacacgatt
     8461 agaaatcgaa gaagttaagc gtccatggct accaccattg ccagaaaatg tatatcaaga
     8521 agatttagta gaaacagatt tcagaaaatt atggtcagat gatgcaaaag aagtggaatt
     8581 aacattagga cttaaagacg taccagaaga acaatatcaa ggaccgatgg tattgcaatt
     8641 gaaaaaagct gggcacatcg cgttaatcgg aagtccagga tatggtagaa caacgttctt
     8701 acacaacatt attttcgatg ttgcaagaca ccatcgtcct gatcaagcac acatgtactt
     8761 gttcgatttc ggtaccaatg gtttgatgcc agttacagac ataccacatg tcgctgatta
     8821 ctttacagta gatcaagaag acaagattgc taaggcgata cgtatattta atgatgaaat
     8881 tgatcgtcgt aagaagattt taagtcagta tcgtgtcact agtatttctg aatatcgaaa
     8941 attaactggt gaaacaattc cgcatgtctt tattcttatt gataactttg acgcagtaaa
     9001 agattcacct ttccaagaag tttttgaaaa tatgatgatt aaaatgacgc gtgaagggct
     9061 agcattagac atgcaagtaa ccttaactgc ttcaagagct aacgctatga aaacaccaat
     9121 gtacattaat atgaaaacgc gtatcgcaat gtttttatat gataaatcag aggtgtcgaa
     9181 cgtagtagga cagcaaaaat ttgcggttaa agatgttgtg ggtcgagcat tgttaagtag
     9241 tgatgacaac gtatcattcc atattggcca accatttaaa catgatgaga ccaaatcata
     9301 taatgatcaa attaatgatg aagtatcggc gatgacagaa ttttataaag gtgaaacacc
     9361 aaatgatatt cctatgatgc cagatgaaat taaatatgaa gattacagag aatcattaaa
     9421 cttaccagat atagttgcaa atggtgcttt accaattgga ttagattatg aaggtgttac
     9481 actacaaaaa attaaattaa ctgaaccagc aatgatttca tcagaaaatc cgagagaaat
     9541 tgcgcatatt gctgaaatta tgatgaaaga aattgacata ttaaatgaaa aatatgcgat
     9601 ttgtatcgca gactcaagtg gagagtttaa agcttatagg catcaagtgg ctaactttgc
     9661 cgaagaaaga gaagacatta aagcgattca tcaactaatg attgaagact taaagcaaag
     9721 agaaatggac ggcccatttg aaaaagatac actttatatt atcaatgatt ttaaaacatt
     9781 tattgattgc acgtatattc cggaagatga tgttaaaaag cttattacaa aaggaccaga
     9841 acttggcttg aacattttat ttgtcggcat tcataaagaa ttaatagatg cttatgataa
     9901 acagattgat gttgcacgta aaatgattaa ccaatttagt ataggtattc gtatttcaga
     9961 ccaacaattc tttaaattta gatttattca acgagaacct gttattaaag aaaatgaagc
    10021 atatatggtc gcaaaccaag cttatcaaaa gattagatgg tttaaatagc aatgaattaa
    10081 ataggaggga ggtatgttat gaattttaat gatattgaaa caatggttaa gtcgaaattt
    10141 aaagatatta aaaagcatgc tgaagagatt gcgcatgaaa ttgaagttcg ttctggatat
    10201 ttaagaaaag ctgaacaata taagcgatta gaatttaatt tgagttttgc actagatgat
    10261 attgaaagca cagcaaagga cgtacaaact gcaaaatcta gtgctaataa ggacagtgta
    10321 actgttaagg gaaaggcgcc caatacgtta tatattgaaa aaagaaattt gatgaaacaa
    10381 aagcttgaaa tgttgggtga agatatcgat aaaaataaag aatccctcca aaaagctaag
    10441 gaaattgctg gcgaaaaggc aagtgaatat tttaataaag caatgaatta atattgaggt
    10501 gaagatatgg gtggatataa aggtattaaa gcagatggtg gcaaggttga tcaagcgaaa
    10561 caattagcgg caaaaacagc taaagatatt gaagcatgtc aaaagcaaac gcaacagctc
    10621 gctgagtata tcgaaggtag tgattgggaa ggacagttcg ccaataaggt gaaagatgtg
    10681 ttactcatta tggcaaagtt tcaagaagaa ttagtacaac cgatggctga ccatcaaaaa
    10741 gcaattgata acttaagtca aaatctagcg aaatacgata cattatcaat taagcaaggg
    10801 cttgataggg tgaacccatg atgaaagatg ttaagcgaat agattatttt tcttacgaag
    10861 aattaacaat tttaggtggt agtaaattgc ctctcgtaaa ttttgaattg tttgatccat
    10921 caaattttga agaagctaaa gctgctttaa ttgaaaagga attagtaaca gagaatgaca
    10981 agttaactga tgcaggtttt aaagtggcta cattagtcag agagtatatt agcgccattg
    11041 taaatattcg aattaatgat atgtattttg caccatttag ctatgaaaaa gatgaatata
    11101 ttttgttaag ccggtttaaa aataatgggt ttcaaatacg aattatcaat aaagacattg
    11161 catggtggtc gattgtacaa tcatatcctt tattgatgag acaagaaaag tccaatgatt
    11221 gggactttaa acaaattgac gatgaaacat tggagaactt aaataatgaa agtatcgata
    11281 cgattgggcg tgttttagaa attgaaatat acaatcatca aggtgaccct caacaaagtt
    11341 tatataacat ttatgaacaa aatgatttgt tattcattcg atacccatta aaagataaag
    11401 tgctgaatgt tcatattggt gtcattaata catttatacg agaattattt ggattcgata
    11461 ctgatgaaaa tcatattaat aaagcagagg agtaatgacg ttgagtggaa aaattagtgt
    11521 taaagctgaa acgattgcac atgttgtaaa agaattggaa agcataagtc aaaagtatga
    11581 tgaaatagct caaaactttg gaaaaatagc gcaattaaat tactacagta gtgaaaaagc
    11641 tgcacattct atggaaaatg gctatagtag tgctgcaaca gtcattagtg gtctcaaagg
    11701 tccattgagt acactcggtg gtggcgtcat gaattcagca caaaagttct ttgaagcaga
    11761 tgaacattgg ggtacggaat ttgccaagct ttactataat attgagggat aggtgcatga
    11821 catgacaaaa gatattgaat atctaacagc tgattatgac aatgaaaagt catctatcca
    11881 aagtgtaata gatgcaatag agggtcaaga cttcttagat gtagatacaa caatggatga
    11941 tgcggtaagc gatgtcagtt ctttagacga agatggcgca atatcattaa caagtagtgt
    12001 agtaggtcca caaggatcta aattaatggg gtattatcaa aatgagttat atgattatgc
    12061 atctcaatta gattcgaaaa tgaaagaaat tattgacacg ccatttatag aagatataga
    12121 taaagcattc aaaggtataa cgaatgttaa attggaaaat atactaatta aaaatggtgg
    12181 tggacatggt agagatacct atggggcttc tgggaaaatt gcaaagggag atgccaagaa
    12241 aagtgacagc gatgtttata gcatcgatga aatattaaaa tcggatcaag aatttgtaaa
    12301 agtaattgat cagcattaca aagaaatgaa aaaagaagat aagaaattat ctaaaagtga
    12361 ttttgaaaaa atgatgactc agggcgcttc ttgtgattac atgacagtag ctgaagcgga
    12421 agagctagag gagcaaaaga aaaaagaaga agctatagag attgcagcac tagctggtat
    12481 ggtagtttta tcttgtatta atcctgttgc tggagcagta gctattggtg cttattccgc
    12541 ttattcagca gcaaatgcag ccacaggaaa aaatattgta actggaagaa agctatctaa
    12601 agaagaacga atcatggaag gactttcgct tattccattg ccaggtatgg gcttcctcaa
    12661 aggtgctggg aaaagtttaa tgaaattagg cttcaaaggc ggagaaaaat ttgcagttaa
    12721 aacaggattg caaaagacaa tgcaacaagc agttagtcgt atttcaccta aaatgggaat
    12781 gatgaaaaac agtgtgttga atcaatctcg taactttgct caaaatactc atgttggaca
    12841 aatgctgagt aacatgcgtg gtcaagcaac tcatactgtt caacaaagta gaaattggat
    12901 tggacaacaa gcacaaaatg tcaaacgaat agtgaataat ggacttgata aagaaatagc
    12961 acatccattt aaacaacaac ttgcaccagc gggaatgggt ggtataaaat ttgctgaaac
    13021 aactactttg agaaacatgg gtcaaaacat aaagcgtgct gttacaccac aaaatcacgt
    13081 gacacatggt ccaaaagata gtatggtgag aagtgaaggc aaacatagta taagtagcca
    13141 tgaaatgaat tcatcaaagt atgttgaatc accaaactac accaaggttg aattcggaga
    13201 acactatgca agacttagac ctaagaaact aaaggcgaat attgaataca caacacctac
    13261 tggtcacata tatcgaaccg atcataaagg tcgcataaaa gaagtttatg tagacaatct
    13321 ctctctaaaa gatggggatc gtaatagcca tgcacaaaga actgtggggg gagaggatag
    13381 attaccagac gatgatggag gtcacttaat cgctagaatg tttggtggtt caaaagacat
    13441 tgataacctt gtggcacaaa gtaaatttat caaccgtcca tttaaggaga aaggtcattg
    13501 gtataatctt gagaaagagt ggcaagagtt cttaaactct gggaaagagg tgaaaaatat
    13561 taaaatggaa gtaaaatata gcggtaatag tcaaagaccg actatattta aagttgaata
    13621 tgaaattaat ggtgaaagaa atattagaag aatattaaat aagtagaggt gccaacatga
    13681 catttgaaga gaagcttagc aaaatataca atga
//
LOCUS       DAKTIP010000056        13705 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAKTIP010000056 DAKTIP010000000
VERSION     DAKTIP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.056"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(74..241)
                     /locus_tag="O0305_001241"
     CDS             complement(74..241)
                     /locus_tag="O0305_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274390.1"
                     /translation="MKRIVILENVIVDNPIWLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(369..872)
                     /locus_tag="O0305_001242"
     CDS             complement(369..872)
                     /locus_tag="O0305_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0274391.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(865..1452)
                     /locus_tag="O0305_001243"
     CDS             complement(865..1452)
                     /locus_tag="O0305_001243"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCY0274392.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGDKNV"
     gene            complement(1464..2264)
                     /gene="racE"
                     /locus_tag="O0305_001244"
     CDS             complement(1464..2264)
                     /gene="racE"
                     /locus_tag="O0305_001244"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCY0274393.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(2500..3315)
                     /gene="sdhB"
                     /locus_tag="O0305_001245"
     CDS             complement(2500..3315)
                     /gene="sdhB"
                     /locus_tag="O0305_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCY0274394.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3315..5081)
                     /gene="sdhA"
                     /locus_tag="O0305_001246"
     CDS             complement(3315..5081)
                     /gene="sdhA"
                     /locus_tag="O0305_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCY0274395.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5133..5747)
                     /locus_tag="O0305_001247"
     CDS             complement(5133..5747)
                     /locus_tag="O0305_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCY0274396.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6071..7852)
                     /gene="uvrC"
                     /locus_tag="O0305_001248"
     CDS             complement(6071..7852)
                     /gene="uvrC"
                     /locus_tag="O0305_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCY0274397.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8018..8113
                     /locus_tag="O0305_001249"
     CDS             8018..8113
                     /locus_tag="O0305_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274398.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8176..8490)
                     /gene="trxA"
                     /locus_tag="O0305_001250"
     CDS             complement(8176..8490)
                     /gene="trxA"
                     /locus_tag="O0305_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCY0274399.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(8663..11011)
                     /locus_tag="O0305_001251"
     CDS             complement(8663..11011)
                     /locus_tag="O0305_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCY0274400.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11021..12733)
                     /gene="polX"
                     /locus_tag="O0305_001252"
     CDS             complement(11021..12733)
                     /gene="polX"
                     /locus_tag="O0305_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCY0274401.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLSADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNT
                     KLKK"
     gene            complement(12806..13327)
                     /locus_tag="O0305_001253"
     CDS             complement(12806..13327)
                     /locus_tag="O0305_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCY0274402.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13328..13594)
                     /gene="zapA"
                     /locus_tag="O0305_001254"
     CDS             complement(13328..13594)
                     /gene="zapA"
                     /locus_tag="O0305_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCY0274403.1"
                     /translation="MTQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4174 a         2632 c         1903 g         4996 t
ORIGIN      
        1 aatttcaaga attttgctac tccatatttt aaccatcaaa ttattgatat aatttatgac
       61 tttagaaatc cttttaaata tgtatacgat tcatgatttt aactaaaatt tcaaatttcc
      121 tgacttcttg gaatcttaat tttatacttt ttaataatac acttttaacc aaataatcaa
      181 catgattcaa ccatataggg ttatcaacaa tcacattctc caaaattaca attcttttca
      241 taacataaat gtgttattca ttccaattat cttataaaat tgtcagaaat acttttaaac
      301 aaacattcat gacacgacct cctagttaaa tcagtctata aactaaaaaa agccatagct
      361 acttctattt aaaaagtagt atgactgatt gtttcgtgat gtcgattttt gaaattgatg
      421 gtgccatgta aagtttgatc atcaataata acttcagcat atgtttcttc cattgaactt
      481 ctagattgag atatacttcc aggattaata acatgaacac cattaatata ctcatatttt
      541 gccacatgtg tatgtccata aaatgcaaac aaacaaccta attcaagtcc tttttcagct
      601 aataaatctc ttgttcgatt gacttgatat aaatgtccat gagtataaaa tgctttcacg
      661 tcattatatt ttgcgaccgc ttcattttca aattctgggt aaaaatcaca attgcctttt
      721 actctattaa ataagctaag ttccgtatca tcatacgcga attctgaatc tcctaaatgt
      781 aaatatacat ctgcatctgg gtgcatttca taaatttgat ataaaacgcc tgattcagta
      841 tggttatcac tcacaataat ccatttagac atttttatca ccttcaagaa aagcttgaag
      901 taaattaatc gcatttcgtc tatgactaat ttgccctttt tgttcttttg aaagttgagc
      961 catggtttta tctaatttcg gtacataaaa tatcggatca tatccgaaac cattttcgcc
     1021 atattttcca tctgcaattt cacctgaaac agtaccttta aatacttttg tttccatatc
     1081 agggccactc atacttatga cacaaacaaa ttgcgcacga cgatcagttg tattaccaag
     1141 cttatttaat aatttttcaa tatttgcttc atcgctttta ttttcaccag cataacgtgc
     1201 agagtatata cctggctcac catttaatgc aaaaacttct agtccactgt catcagctat
     1261 gaccgtttta ttcaatgctt ttgcagcagc ttctgatttt aatatagcat tttcttcaaa
     1321 tgttgatcct gtttcttcca catcaaaatc tggtattagt tctgaaatac ctattacgtg
     1381 gtaatctgga aatattactt taaagtcatt tattttccct tgattattcg atgcaataac
     1441 aatctctttc attaaaaatc ctcctagtca ttcactgata tacgttccac attgacagat
     1501 aaatttaacc actctttgat aatgttagta atgtgagtag gatcacctgt tgcaaaaaat
     1561 cgatgatctg gatgttcagt ataacttgca tgttcattac taaatgttag caatgcacta
     1621 acttcacgag ccgtttctaa tccagacgaa atcactgtct ttttaccacc aaaataatca
     1681 tagataggtt tatagagcaa tggatagtgg gtacatccta aaatgacagt atcagactca
     1741 ctattacgcc aacgtttcag tgtttgatga atgacaatgc ttgtaattgt tggatcacta
     1801 tatctcattt gttctacaag tggcacaaaa cctggacagg caacgccatg tacctctaca
     1861 tgtggattga tacgtttaat atgcgtacga tatgcttcag atttaattgt gccttccgtt
     1921 cctagtacta atacattttg atttctagta gtcattattg ctgttctagc acctggttca
     1981 attacgccaa tcactgggat tgataaggtc ttttgtaaat attctaaagc tacagcagtt
     2041 gctgtattac aagcaatcac gagcattttt atatcaaatt ccattaattt acgagcgatt
     2101 tcaactgtat attgttttac ttgttctcct ggtcttggcc catatggaca tcgtccaata
     2161 tcacctaagt aataaatcgt ctcatttggc aactgacgca taatttcttt agctactgtc
     2221 aaacctccga caccagagtc tattacacct attggtttat tcatattcgg tcatccttac
     2281 ttttaattta atttttagtt tatcataact aagcattgga ttttagtatt atgcactgtg
     2341 tttaccattt ttgtcattat aatatttatt ttaaatcagc ccactatcat attgtcatgt
     2401 aatctgctta ttaaaaaaat cccttccaag ttattgtgta tctccattca atttaatttt
     2461 gaaaggaaca taacatttta actcaaaagg gattaatttt tattctactt catggtctga
     2521 accaaagaat gatttaaaca tgtggaatgt tgtttctctg ttcattgctg caatggatgt
     2581 tgttaatgga atacctttag ggcaagcatt aacacagttt tgtgaattac cacactgctg
     2641 taagccacca gtacccatta atgcatttaa acgttcatct ttagtcatag atcctgttgg
     2701 gtgcaaatta aacaaacgaa cttgcgagat tgcttgtgca ccaacgaatt tattattttc
     2761 agtaacatta ggacaaacct ctaaacatac accacatgtc atacatttag ataattcata
     2821 agctgtttga cgttttttct ctggcatacg tggtcccgga cctaaatcat acgttccatc
     2881 aattgggatc catgctttca tacgttttaa gttatcgaac attctagaac gatcaacttg
     2941 taagtcacgg ataactggga aagtattcat tggctctaaa cgaataggtt gttctaattg
     3001 atcaacaatc gcagaacaag attgtcttgc acgaccattg ataaccatag aacatgctcc
     3061 acatacttct tctaagcagt tcatatccca gacaacaggt gttgtttttt cacctttaat
     3121 attaactggg ttacgtctaa tttccattaa acaagcaatg acgtttaaat tttcacgata
     3181 tggaatttca aatgtttctt cataaggctt agaatcactt gtatcttgtc gtttaataat
     3241 taattttact gttttttgtt tcggtttaga ttgtgtttca tgttgtggag tgtttttcac
     3301 tgattgttca gtcattattt tttaccccct ttagacttac ttgtgtaatc acgtttacga
     3361 ggtggtatta aactcacatc gacgtcatca taagtaaact gcggtttttc aaatgcgcct
     3421 tggaatgagg ccattgtcgt ttttaaccac tcttcatcat tacgctctgg gaattctggt
     3481 ttataatggg caccgcgtga ttcgttacgg ttatatgcac caatcgtaat aacacgtgca
     3541 agtactaaca tgttccatag ttgacgggta aagaataccg cttggttact ccaagtttga
     3601 gtatcttcca tatcaatatc ttcataacgt ttcatcaatt caacaatctt tttatctgtt
     3661 tctaacagtt tttcattttc acgaacaaca gttacatttg ctgtcataat ttcaccaagt
     3721 tcacggtgta atttatatgc attttctgta ccgcgcatag ctaataattt atcaaaacgt
     3781 tcttgctctt cagctttacg cttttcaaaa atactttcgt ccatatcagt atatgatcga
     3841 tcaatatttg aaatataatc aatcgcgttt ggacctgcta ctgtaccacc ataaatcgct
     3901 gataacaatg aattggcacc taagcggtta ccaccatgtt gagagaagtc acattctcca
     3961 gctgcaaata accctttaat atttgtcatt tgatcataat ctacatatag accacccatt
     4021 gaatagtgaa cagctgggaa aatcttcatt ggtactttgc gtgggtcatc accagtgaat
     4081 ttttcataaa tctcaatgat accacctagt tttacatcta actcatgtgg atctttatgt
     4141 gacaaatcaa gatataccat gttttcgcca tttataccta atttttggtt aatacataca
     4201 tcgaaaattt cacgcgttgc gatatcacga ggtactaagt taccataatc aggatatttc
     4261 tcttctaaga agtaccaagg cttaccatct ttatatgtcc aaattcgtcc accttcacca
     4321 cgtgctgatt cactcattag tcgcagttta tcatcaccag ggattgcagt aggatgaatt
     4381 tgaatgaact caccattagc ataaatagcg ccttgttggt aaacaatgga agccgctgat
     4441 cctgtattaa tcattgagtt tgttgtttta ccgaaaataa taccagggcc acccgttgcc
     4501 ataataactg catctgaacc aaatgtttca atctcagcag ttgtcatatt ttgtgcaacg
     4561 atacctcttg cactatcatc gtcaccttta actatgccaa ggaattccca tccttcatac
     4621 ttcgtaacta atccatctac ttcatatgca cgaacttgtt catccaatgc atataataat
     4681 tgttgtccag ttgttgcccc tgcatatgct gttctgtgat gtaatgtacc accgaaacgt
     4741 ctaaaatcta atagaccttc atttgttcta ttgaacatta cgcccatacg gtctaataaa
     4801 tgaataattt taggtgctgc ctctgtcatc gctttaacag gtggttggtt tgcaaggaaa
     4861 tcgccaccat acactgtatc atcaaagtga atccaaggag aatcgccttc ccctttagta
     4921 ttgaccgcac cattaatgcc accttgggca caaacagagt gcgaacgctt tactggtaca
     4981 actgagaaca aatctacatg tgcacctttt tctgccgctt taattgttga cattaagccc
     5041 gctaggccac ctccgacaac aataagatgt ttctctgcca taaaaatttc actcccctaa
     5101 attttcaatc tatatttgtt aaatgcgatg tattacataa aggcaataat tgcagtaaca
     5161 ccaatatacg aaataactaa aaatacgatt aatgaaaccc atgtaaatac tcgttgtgat
     5221 tttggagatt gaagtccacc ccaagtaact aagaatgacc ataagccatt tgcaaagtgg
     5281 aacacaacag caataataca aataatataa aatattgccc atccaggatg ttgcaatgtt
     5341 tcgtgcatta aatcgtaatt cacttctttg ccgtaaaatg ctttttgtaa acgtgtttgc
     5401 cataaatgga taccaataaa gataaatgtt aagataccac tcactctttg gaagaagaac
     5461 atccagtttc taaaaatcga gtaatgtcca acattttctt ttgctgtaaa tgcaatgtgt
     5521 ataccaaaca aaccgtgata taacaacgga atgtatataa ataaaaattc tacaataatt
     5581 agaaatggta atgattccat aaagttagat gccttattaa acgcttcagc accttgtgtt
     5641 gcttggtgat tcactaataa atgaacgacc aaaaatgcac ctattgggat aatacctaat
     5701 aacgagtgaa tacgtcttag ataaaattca ttttttgatt gagccaaaag gagtcccccc
     5761 tgtgaacgaa tatttaattt attgagctat ttatattaaa cgtacgctta accccctaaa
     5821 gtgatatcgt ttctagcgtt gtcattatca ttaagcgaaa cattttaaag acaaatacac
     5881 actgcacgat caccaaactg catgtcgaac aatgtaacat ttggattcga tatttaaaat
     5941 tgcttgtgat gataaacttt ctcatttaga aaacgcttcc acgtacattc aaaaaaataa
     6001 ctttgttaac catattgtaa cattatttca tatattttgg ggcatgagaa tgattctcac
     6061 gcccagtaat ttatttatgc aattgttcat gtaggttctt tgcgacgttt tcaggaatac
     6121 ctatattttt aaaatcttca agtgtagctt ccttcatttt cttgattgaa ccgaatgaac
     6181 gcaataataa tgttttacgt ttgttaccga taccatctat atcatcaagt attgatttca
     6241 agcctgtctt ttgacgtgtt tgtctatgaa atgtgattgc gaatctgtga acctcatctt
     6301 ggatacggtg caacaaataa aatgcctggc tatttttctt cagtggtaca atttctgcac
     6361 tagcgccata taataattca gatgtttggt gtttatcatt tttctgcaaa cctgcaacag
     6421 ggatatcaag acctaattcg ttttgtagca catcaataac cccgttcata tgtcctttac
     6481 caccatctac tattattaaa tcaggtaatg gtaatccttc gtttaaaacg cgagaatatc
     6541 gtcgtcttac tacttctctc attgatttgt aatcatctgg acctttaacc gttttgattt
     6601 tatactttct ataatttttc ttatctggtt taccgtcgac aaatgtaacc attgctgaca
     6661 ctggatccac accttgaata ttagaattat cgaatgcttc aattctaatt ggtgtttgaa
     6721 ttcccatttg tgttccaagt tcttcaatag ctttaatcgt tctggactca tcacgtgata
     6781 ttaattcaaa tttattattt aaggatactt tagcgttatg tgcagctagg tcaaccatat
     6841 cttttttggg acctcgcgcg ggttgaacga ttttagtgtc cacaacagat tgaatcattt
     6901 ctttatccaa attacgtggt acatgaactt ccttaggtaa aatatgttgg tttaagctat
     6961 aaaattgtcc aataaatgta taaaattctt cttcttctgt ttgctgtaat ggaatcatcg
     7021 ttgtatctcg ctttatcata ttaccttgtc gtataaagaa aacttggata cacatccatc
     7081 ctttatcaac actataacca aagacatcac gaatcgtttt atctgatgac ataatttttt
     7141 gtttgtttgt cagattttga atatgttgaa ttaaatctct atattcttta gcccgttcaa
     7201 aatcaagtga ttcacttgca gttaacattc gctcttctaa actttttaaa attgttttgt
     7261 cttccccatt cagaaaatca gtaatttcct tcgtcatttg tgcgtattta ctcaaatcaa
     7321 cgtcatatac acatggtcct aaacattgtc caatatggta ataaagacat aatttatctg
     7381 gcatcttatc acatttgcga tatggatata ttctgtctaa taacttttta gtttcttgag
     7441 cagaatatgc attcggatac ggtccgaaat atttgccagt accttgtttt acagttctcg
     7501 tcactagtag tctaggatat ttctccttcg taattttaat aaatggataa cttttatcat
     7561 cctttaataa tatattatat cttggttgat attgtttaat cagattcaat tccagtaaaa
     7621 gtgattctgt ttcacttgac gtgacaataa actcaaagcg acgtatttca ccaaccagtc
     7681 tcgttgtttt agcatcatga gcacccgtaa aatatgatcg caatcgattt cttagctttt
     7741 tagctttgcc aacatatatc acttgatcat tacgatcttt cattaaatag catcctggtt
     7801 ccataggtac gacatttaat ttatttttaa ttcgttgctt atagtcttcc aaaaatatcc
     7861 ccccttctaa ttttaaaatg aatgatgcta caaaagtgct tcaaatttat caccactaat
     7921 tctagttaat aatcaacatc ttcgagtatg acttttcatg cacatcatgt aatattgaac
     7981 atggttttat caaaccaact gctacaaatc tatcagcatg ctagattatc atttaacgtt
     8041 catagattta ttaatcccac cattctcaag tcgtaaacaa tatgatgact tatcattcaa
     8101 ctttttatat taaaaacaca aacaaaaact ggagcaattc attaaagaaa tgccccagtc
     8161 gtcattggtt gtaacttata aatgtttatc taaaacttca gctaagtttt cttttggttg
     8221 gaaaccaaca actttatcaa ctggttgacc gtctttaaag acgattaatg ttggaatact
     8281 catcacttca tatttagctg cagttgatgg attttcatca acatctaatt ttaaaatgtc
     8341 agctttacct tcatagtcag ctgctaattc ttctaatacc ggagcgatca ttttacatgg
     8401 accacaccat gttgcccaaa aatctactag ttgtacacca gattctactt ttgaatcaaa
     8461 atctgcatct gttactttta cgattgccat aaatgccaat cctccttaat tattattatg
     8521 atgttcaaat tatatcagat aacatgcttc atgtatttct atttgctcaa tttttatgct
     8581 tatttcaaat aacatgtttg aaatgtattc ttgttgcttt acattatttt aactgcagct
     8641 atttaattta tcaaattata atttatttta gtgttgcaac ggtaacgcca aatccacctt
     8701 cgcttggcat accacctcta aagtcactaa cacttttatg ctttttcaaa tgttgttgta
     8761 cacctttttg aagtgcacct gtacctttac catgaatgat ataaacttgt tcgtaattac
     8821 ttaaaacggc ttgatctaaa tattgatcta gttcaattaa agcatcctca taacgatagc
     8881 ctcgtaagtc aagttcagtt ttaattgttt gacgattttg acgtgtaacc attttcgttg
     8941 gcttaacttt ttctttttgt tttttctcta aatcttcaat aggtaacttc attttaataa
     9001 ttcccatttg aacaattgct tcttcatcat tgacaatttc taaaacttca cccttttgac
     9061 cgtaagataa tacttttact tcatcaccag caacaatttt atcgtatttt tgcttttgta
     9121 cattttgctt tatagatttc gcttcataat gatcatctaa tcgtttcttc ttatcaatca
     9181 attcatgttc tttaacatct gcaccttttt gttcacgcaa ttgtcttaag tctttaataa
     9241 tatcgtcagc ttcttttgtt gcagccttaa tcttctgatt tgctttttct ttcgcttcct
     9301 ctattagaga cttttcataa ttttggaatt gttgatactg cttagataaa tcatcatgca
     9361 cttgctccgc ttcttttaca agacggtcca gttctaacct ctgtgtctct acacgtttgt
     9421 aattacgctc taatgattca atcatttcat ttatttcttt ttcatcagta ccaatcatcg
     9481 tcttagcctt attaataata ttcaaactaa gacctaactt tttagaaatg tcaaaagcat
     9541 ttgaacgacc cggcacaccc attaataact tatacgttgg actcaaagta tctacatcaa
     9601 attctacact cgcattcata acgccttctc gattataact atatgcttta agttcaggat
     9661 agtgcgtcgt tgccattact agagaaccaa tttttctaac atgatctaaa atgctcattg
     9721 ctaatgcagc accttcactc ggatctgtac ctgcacctaa ttcatcaaat aaaactaaac
     9781 tatgtttgtc tgcatgcttt aaaatttcaa ctatattcgt catatgagat gaaaaagttg
     9841 ataatgattg ttctattgat tgttcatctc cgatatcgca atatacattt ttaaatacac
     9901 ttaactgact accatcaagt gtgggaatca acaatcctga ttgagccata acaataatta
     9961 aacctaatgt ttttaatgtt acagttttac cacctgtatt cggtcctgta ataattaccg
    10021 tttcaatatc ttccataaat tcgatggtat tagctacaac agtctcacga tttaataatg
    10081 gatggtatgc tttaggtaaa tatacagtac ggtcctcttt aaatatcggc tttgttcctt
    10141 taatacttct actatatctc gcttttgcga ttaaaaaatc taactgaccc atgacttgtt
    10201 ctgccacaag tagtgcatct ttgtccgcag ccacataacc agttagttgc gttaaaatgc
    10261 gttctttttc aattgcttcg tcatgacgta atcgactaat ttgattattc atttcaacaa
    10321 ctgatgatgg ctcaatatac aatgtttgtc ctgaagcaga ttgatcatgt acaatcccat
    10381 taaaatcttg tcgatattca gctttgacag gtataacgtt tctttcattc ctaactgtta
    10441 caatagcatc tgataatttt ttctgatttg cttggctttt aacaatacgg tccaaatttt
    10501 gtctaatacg ttgattcgtg ctagaaattt tacttctaat cccttgcaat tcataactcg
    10561 cattatcata taaatcatac gtatcgcatg tttcatttat ttgttgaaaa agatcagtta
    10621 acacaggtaa ttgattcatc ttgtcatcta atattgggta tttaacacct tcatcttctt
    10681 caaccaattg attataaaat gtcttgaatt gattttgtac ttgaattaat ctttttatca
    10741 agttaagctc tgatacattt aaaacgccgc caatatcagc gcgatgaatg aatgctgata
    10801 ctttagataa gccactcaag cttggtaaac gatgcttatt atagatttga gcaatctcat
    10861 ccgtttcttc catttgaaaa acaaccgttt caaaattagt agctggcatc atttgattga
    10921 ccttttccaa gcctaagtca ctaatagttt cattggcaac gagtgatttt attttttcaa
    10981 attctaagac gtctaatgtt ttttgtctca taaaatccct ctatttctta agttttgtat
    11041 tattttcaat aaagtcttta aaagcttcac gcgataatgc gttaatcact ctatcttttg
    11101 ttacaaatcc tttttgcgca gttgctacgc cataattcat aaaatctaaa tgatttgtat
    11161 gatgcgcatc agtgttaata gttaatttca catttggata tttacgaacg atatcagcgc
    11221 tcagatccag tcgatgtgga ttggcattaa tttctaatac tgtattcgtt tcttcagcta
    11281 atgccattaa ttgttcaata ttcggtttat aaccatctct tctacctata atacgccctg
    11341 ttggatgcgc tatatgtcgc acgtatggat tgcgacatgc attagctaat cgttccataa
    11401 tttgttcttc tgattggtta aagctttgat gaatagctcc aattacataa tcaagttgtg
    11461 ctaaaatttc atcatcataa tccagcgagc catcaggtaa tatatccatt tctgtacctg
    11521 aataaatatc aatttcacta tattctttat ctaaagcctt aatttcttcg ttttgtctta
    11581 aaagtctttc tacttgtaag ccattagcaa cacgtaaact ttgtgaatga tcagtaatta
    11641 ccatgaattt ataacctttt gcgatatttg cttctaccat gtctcgaata gaaaacgcac
    11701 catcactata cgttgtatgc atatgaatat caccattaat atcatctatt gtaatgatat
    11761 tacttagatc tttatcaaat tcgctaccat cttctcgcat agcaggtggt ataaaattca
    11821 cattaaaatg ttcatatatc ttggcttcac tatcatattg aattaatgta ccatcagctt
    11881 gttcaattcc atattcactt actttttcat cacgtgcttt agcaagttgt cgaattctta
    11941 tattatgttc ttttgaccca gtaaaatgct gcaatgtatg ataaaaagca cttggttcaa
    12001 ttaatcgaaa atcgacacca atcgtttcat catcatacgc taattctaat gaaacttttg
    12061 tgttccccac tgcaacttct tttactttat tgggaatatt taataattgc tgctgcactg
    12121 cttttgggtt atcggtactt attatgaaat ctaaatcttt gctcatttct ttaaaacgac
    12181 ggaagcttcc tgcagatgaa tattgatcga tataatttaa tgtatctata taatcaatga
    12241 tttcttgatt aagtcttctc atttgatcaa ttggatatct atctttctta gcaccaagtt
    12301 gtttcacagc ttctaatatg ttttgttccg ttttcttagc aaatccgctt aattcactaa
    12361 cttttccatt ttcacaagca acttgaagtg acgctttatc aacaatattc aactctttat
    12421 atagcttagc aattttcttg cttccaagtc cttgaatttt caaaagtgga ataagacctt
    12481 ccggaacttc ttcctgtaat tgctgtaaat actgagattc accggtctca cggtaatcat
    12541 tgattacttc tgcaacacct ttaccaatgc cttttaactc cgttacatca gatatttcat
    12601 ctaatggtcg ttcatctaat tcaagacttt gagctgcttt tcgatacgct gatattttaa
    12661 aagtattttc cccttttaat tccatataag tagcaatttg ttctaatagt ttgataacat
    12721 ctttttttgt cataataaca ctccataaaa agaagaccag gacgtatcat taatatatac
    12781 ctttgtcctg acctcttatg ataatttata aattaatggt gaaagccgat aaatatggga
    12841 tgtgaaaaat caatgaatga ctcacaatag atattttaag ctgatgttgt aatgcttcgt
    12901 taggatataa tgccaataga tataacgtaa agtgtaagac aattatcgtc ataaacacac
    12961 caactatcat tcccattgca cgactgaaaa tatgaatgtt ttgatacgct attattttat
    13021 caaaagttac gataattaga tataaaatga acttacaaaa caatgtaatc attaaaaaag
    13081 ctacaatcgc ttcaaatcga ttttgtagat gattaaaatg aaacgcaaaa gttgtattaa
    13141 atgctgttgt tttaggatat ggaataaata caattaatct ttctacaata gatttgtaaa
    13201 attgactggc aatccacaat gatacaatcg ttgcactcaa atgtatcata gataaccaaa
    13261 aacctcgtct gaatccaacg atgacaaaat acacaaagaa aatgattatg ataaaatcaa
    13321 tgaccattta ttgctcacgc tgctgcaatt tgtgaatttg ttgtttcaaa cgtcgatttt
    13381 cttcttctag tagtactttt tcatgcataa tattcacagc agttagtatt gcttttcttg
    13441 aagtatctaa acctgctgct ttatacccta attcttttat tttatcatca actaaatgtg
    13501 ctacatatcg tatgtgctct gggttatctt ccccaacaat tgtaaaaagc tgatcattaa
    13561 ttgatacatt taccttgttt ttaaactgtg tcatttataa tttctcctga tccttttttt
    13621 aaaatctaaa ttcacgttat aaaatatgac tggatagttt gtctgaattt gatactaata
    13681 ttgttatatt gcaattatga taaaa
//
LOCUS       DAKTIP010000057        13669 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAKTIP010000057 DAKTIP010000000
VERSION     DAKTIP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.057"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            29..2560
                     /gene="secA"
                     /locus_tag="O0305_001255"
     CDS             29..2560
                     /gene="secA"
                     /locus_tag="O0305_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCY0274404.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            2602..2706
                     /locus_tag="O0305_001256"
     CDS             2602..2706
                     /locus_tag="O0305_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274405.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKKSV"
     gene            2875..3985
                     /gene="prfB"
                     /locus_tag="O0305_001257"
     CDS             join(2875..2946,2948..3985)
                     /gene="prfB"
                     /locus_tag="O0305_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCY0274406.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     INFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            4139..4246
                     /locus_tag="O0305_001258"
     CDS             4139..4246
                     /locus_tag="O0305_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274407.1"
                     /translation="MCLLKQHMQCAFSNITYGDFQGIELIIENVLNYMV"
     gene            4394..5233
                     /locus_tag="O0305_001259"
     CDS             4394..5233
                     /locus_tag="O0305_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0274408.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVTKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            5407..6057
                     /locus_tag="O0305_001260"
     CDS             5407..6057
                     /locus_tag="O0305_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0274409.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            6054..6290
                     /locus_tag="O0305_001261"
     CDS             6054..6290
                     /locus_tag="O0305_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCY0274410.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            6559..8544
                     /gene="uvrB"
                     /locus_tag="O0305_001262"
     CDS             6559..8544
                     /gene="uvrB"
                     /locus_tag="O0305_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCY0274411.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            8552..11398
                     /gene="uvrA"
                     /locus_tag="O0305_001263"
     CDS             8552..11398
                     /gene="uvrA"
                     /locus_tag="O0305_001263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCY0274412.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     HDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            12060..12992
                     /locus_tag="O0305_001264"
     CDS             12060..12992
                     /locus_tag="O0305_001264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCY0274413.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            12998..>13669
                     /locus_tag="O0305_001265"
     CDS             12998..>13669
                     /locus_tag="O0305_001265"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0274414.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGSVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGL"
BASE COUNT         4983 a         1903 c         2717 g         4066 t
ORIGIN      
        1 cagtttttta gctaaaggag cgaacgaaat gggattttta tcaaaaattc ttgatggcaa
       61 taataaagaa attaaacagt taggtaaact tgctgataaa gtaatcgctt tagaagaaaa
      121 aacggcaatt ttaactgatg aagaaattcg taataaaacg aaacaattcc aaacagaatt
      181 agctgacatt gataatgtca aaaagcaaaa tgattattta gataaaattt taccagaagc
      241 atatgcactt gttagagaag gctctaaacg tgtattcaat atgacaccat ataaagttca
      301 aattatgggt ggtattgcaa ttcataaagg tgatatcgct gagatgagaa caggtgaagg
      361 taaaacatta acagcgacaa tgccaacata cttaaatgca ttagctggta gaggtgttca
      421 cgttattaca gtcaatgaat acttatcaag tgttcaaagt gaagaaatgg ctgagttata
      481 taacttctta ggtttgactg tcggattaaa cttaaacagt aagacgacag aagaaaaacg
      541 tgaagcatac gcacaagaca ttacttacag tactaataat gagctaggtt ttgattactt
      601 acgagataac atggtgaatt attctgaaga tagagtaatg cgtccattac attttgcaat
      661 cattgatgag gttgactcaa ttttaatcga cgaggcacgt acgccattaa ttatttctgg
      721 tgaagctgaa aagtcaacgt cactttatac acaagcaaat gtttttgcga aaatgttaaa
      781 acaggacgaa gattataaat acgatgaaaa aacgaaagct gtacatttaa cagaacaagg
      841 tgcggataaa gctgaacgta tgttcaaagt tgaaaactta tatgatgtac aaaatgttga
      901 tgttattagt catatcaaca cagctttacg tgcgcacgtt acattacaac gtgacgtaga
      961 ctatatggtt gttgatggcg aagtattaat tgtcgatcaa tttacaggac gtacaatgcc
     1021 aggccgtcgt ttctcggaag gtttacacca agctattgaa gcgaaggaag gcgttcaaat
     1081 tcaaaatgaa tctaaaacta tggcgtctat tacattccaa aactatttca gaatgtacaa
     1141 taaacttgcg ggtatgacag gtacagctaa aactgaagaa gaagaattta gaaatattta
     1201 taacatgaca gtaactcaaa ttccgacaaa taaacctgtg caacgtaacg ataagtctga
     1261 tttaatttac attagccaaa aaggtaaatt tgatgcagta gtagaagatg ttgttgaaaa
     1321 acacaaggca gggcaaccag tgctattagg tactgttgca gttgagactt ctgaatatat
     1381 ttcaaattta cttaaaaaac gtggtatccg tcatgatgtg ttaaatgcga aaaatcatga
     1441 acgtgaagct gaaattgttg caggcgctgg acaaaaaggt gccgttacta ttgccactaa
     1501 catggctggt cgtggtacag atatcaaatt aggtgaaggc gtagaggaat taggcggttt
     1561 agcagtaata ggtacagagc gacatgaatc tcgtcgtatt gatgaccagt tacgtggtcg
     1621 ttctggacgt caaggtgata aaggggatag tcgcttctat ttatcattac aagatgaatt
     1681 aatgattcgt tttggttctg aacgtttaca gaaaatgatg agccgactag gtttagatga
     1741 ctctacacca attgaatcaa aaatggtatc aagagctgta gaatcagcac aaaaacgtgt
     1801 agaaggtaat aacttcgacg cgcgtaaacg tatcttagaa tacgatgaag tattacgtaa
     1861 acaacgtgaa attatctata acgaaagaaa tagtattatt gatgaagaag acagctctca
     1921 agttgtagat gcaatgctac gttcaacgtt acaacgtagt atcaattact atattaatac
     1981 agcagatgac gagcctgaat atcaaccatt catcgactac attaatgaca tcttcttaca
     2041 agaaggtgac attacagagg atgatatcaa aggtaaagat gctgaagata ttttcgaagt
     2101 cgtttgggct aagattgaag cagcatatca aagtcaaaaa gatatcttag aagaacaaat
     2161 gaatgagttt gagcgtatga ttttacttcg ttctattgat agccattgga ctgatcatat
     2221 cgacacaatg gatcaattac gtcaaggtat tcacttacgt tcttatgcac aacaaaatcc
     2281 attacgtgac tatcaaaatg aaggtcatga attatttgat atcatgatgc aaaatattga
     2341 agaagatact tgtaaattca ttttaaaatc tgtagtacaa gttgaagata atattgaacg
     2401 tgaaaaaaca acagagtttg gtgaagcgaa gcacgtttca gctgaagatg gtaaagaaaa
     2461 agtgaaaccg aaaccaatcg ttaaaggcga tcaagttggt cgtaacgatg attgtccatg
     2521 tggtagtggt aaaaaattca aaaattgcca tggaaaataa atgatataaa ataactcctt
     2581 ccaattaaac acctatagtt tgtgttatgg gaggagtctt tttattttac aagcgttaaa
     2641 tactttaaaa aatgtgaaga agttgttaaa cgttgttatg tacttagttt taaaaaatcg
     2701 gtttaggcat atgtcgatga taaatgtact gatttttaac aataaatgca taaactaatt
     2761 gtcagtgtgc ttatatttct taacattgtt atttaacaaa attatgttaa aatttagcat
     2821 tataaaagat gcaaatcaat gacttgaatt gaaatataaa taggagcgaa tgctatggaa
     2881 ttatcagaaa tcaaacgaaa tatagataag tataatcaag atttaacaca aattaggggg
     2941 tctctttgac ttagagaaca aagaaactaa tattcaagaa tatgaagaaa tgatggcaga
     3001 acctaatttt tgggataacc aaacgaaagc gcaagatatt atagataaaa ataatgcgtt
     3061 aaaagcaata gttaatggtt ataaaacact acaagcagaa gtagatgaca tggatgctac
     3121 ttgggattta ttacaagaag aatttgatga agaaatgaaa gaagacttag agcaagaggt
     3181 cattaatttt aaggctaaag tggatgaata cgaattgcaa ttattattag atgggcctca
     3241 cgatgccaat aacgcaattc tagagttaca tcctggtgca ggtggcacgg agtctcaaga
     3301 ttgggctaat atgctattta gaatgtatca acgttattgt gagaagaaag gctttaaagt
     3361 tgaaactgtt gattatctac ctggggatga agcggggatt aaaagtgtaa cattgctcat
     3421 caaagggcat aatgcttatg gttatttaaa agctgaaaaa ggtgtacacc gactagtacg
     3481 aatttctcca tttgattcat caggacgtcg tcatacatca tttgcatcat gcgacgttat
     3541 tccagatttt aataatgatg aaatagagat tgaaatcaat ccggatgata ttacagttga
     3601 tacattcaga gcttctggtg caggtggtca gcatattaac aaaactgaat cggcaatacg
     3661 aattacccac cacccctcag gtatagttgt taataaccaa aatgaacgtt ctcaaattaa
     3721 aaaccgtgaa gcagctatga aaatgttaaa gtctaaatta tatcaattaa aattggaaga
     3781 gcaggcacgt gaaatggctg aaattcgtgg cgaacaaaaa gaaatcggct ggggaagcca
     3841 aattagatca tatgttttcc atccatactc aatggtgaaa gatcatcgta cgaacgaaga
     3901 aacaggtaag gttgatgcag tgatggatgg agacattgga ccatttatcg aatcatattt
     3961 aagacagaca atgtcgcacg attaatatat attttaaaac cgaggctcta aaagggcgtc
     4021 ggtttttggt ttttttaaag gtagctaaat aaattgtaaa ttagattttg gaatatgatt
     4081 tgtttatgaa tatttaagta caattcggta gatagagtta gaatatattt tttaaaagtt
     4141 gtgtttgtta aaacaacata tgcagtgtgc atttagtaat attacctatg gcgattttca
     4201 aggtattgaa ttaattattg aaaacgttct caattacatg gtatgaatac attttacact
     4261 atgataaaag gttgtattct ttttatattg ttaaccattt gattacatcg ttataacaat
     4321 agcttttgac aaaatgtatt gtgctatagt atttgcatac ttaaaatact aacagcaaag
     4381 gaatgacagc aagatgaaaa aatctcttac agtgacggtt tcgtcagtgt tagctttttt
     4441 agctttaaat aatgcagcac atgcacaaca acatggcaca caagtaaaaa cacctgttca
     4501 acataattat gtatcaaatg ttcaagcaca aacgcaatca ccgacaactt atacagtagt
     4561 tgctggcgat tcattatata agattgcttt agagcatcac ttaacgttga atcaattata
     4621 ttcatacaat cctggtgtaa cacctttaat ttttcctggt gacgtgattt cacttgtgcc
     4681 tcaaaataaa gtgaaacaaa ctaaagcggt taaatcacca gtaagaaaag caagccaagc
     4741 taaaaaggta gtaaaacaac ctgtacaaca agcatctaaa aaagtagtag ttaagcaagc
     4801 acctaagcaa gcagtaacta agacagttaa tgtagcatac aaacctgctc aagtacaaaa
     4861 atcagtacca actgtacctg ttgcacataa ctacaataaa tcagttgcta acagaggaaa
     4921 cttatatgct tatggaaact gcacatatta tgctttcgat cgtcgtgcac aattaggtag
     4981 aagtatagga agtttatggg gcaatgcaaa taactggaat tacgcagcaa aagttgcagg
     5041 atttaaagta gataaaacac cagaagttgg cgctattttc caaacagctg ctggtccata
     5101 tggacatgtt ggtgttgttg aatctgtaaa ccctaatgga acaattactg tttctgaaat
     5161 gaactatgct ggatttaatg ttaaatcttc aagaacaatt ttaaatccag gaaaatataa
     5221 ttacatccac taagtaatat atcaagacaa gactatcctc ttagcctgtt taagtaacag
     5281 gttgagagga ttttttggta tcatttaatc agagttatat aaagaagata tttaaatgat
     5341 atttaattga ttgatatgta aaaagaaagt ataatttatg attaattaat ggaggaggta
     5401 attgaaatgg gtgtacatca atattttaaa agattatcag atatggaaag acttataaga
     5461 ttacctggaa aatttaaata ttttgaacac aatgttgcag cacactcctt taaagtaact
     5521 aaaattgcac aatatctagc aacagttgaa gaatatcatg gacgaaagat taattggaaa
     5581 agcttatatg aaaaagcatt aaatcatgat ttcgccgaag tgtttactgg tgatataaaa
     5641 acacctgtta aatatgcgag tagtgaatta aaaaaattat tttcgcaagt tgaagaagaa
     5701 atggtagaga cctttattga agaagaaatt ccattacaat atagagatgt ttataagcaa
     5761 cgactgcaag aaggtaaaga tgattcatta gaaggccaaa tactttcagt tgctgataaa
     5821 attgatttgc tttatgaaac atttggagaa atacaaaaac gtaatcccga agaattattt
     5881 ttcgaaattt atgaaatgag tctagaaaca attattcaat ttgaccattt agcatctgta
     5941 caagatttta ttaataatat cattccagaa atgttgactg aaaactttat acctagaaca
     6001 gaattaagag aaacaaccat gaacatttta aataaaagaa aagaggaaaa tgaatgatat
     6061 ggtattttag cgcagcattc tttccatgtg tcttggtagt attatttagt gtaataacaa
     6121 gaagtaaatg ggtcggtact attctgacat taattttaat tggtgcctca atctataaag
     6181 agtatttcca taacgagtgg attattttta ttgatgtagt gtcattatta gctggttatt
     6241 taattataga tcaactcgaa tttcataaac atcaagatga agatcgctaa gattaacttt
     6301 aaaataatgt ttcaaacaaa tttgttgaaa caaaatgatg attaatataa tgtgtattta
     6361 catactaaaa ataacaagat aaacgatttg atttaaggca agcatagtta gcacaactat
     6421 gtttgttttt ctttgttcga catttttacg aacaaacgtt tgctttttgt gtgactactt
     6481 tgctaaaata tgtaatgaga aagcaaagag ttgagcggaa atataaataa tacgttgaaa
     6541 gaggaggcat atgtgacaat ggttgaacat tatcctttta aaatacattc tgattttgag
     6601 cctcaaggtg accaaccgca agcaattaaa gaaatcgtgg aaggtattaa agcggggaaa
     6661 agacatcaaa ctttattagg tgctactggc acagggaaaa catttacgat gagtaatgtt
     6721 attaaagaag ttgggaaacc aacgttaatt atcgcgcata acaaaacatt agcaggacaa
     6781 ttatatagtg agtttaaaga attttttcct gaaaacaggg tggaatactt tgtaagttac
     6841 tatgattatt atcaaccaga ggcatacgta ccgtctactg acacttttat tgaaaaagat
     6901 gcctcaatca atgatgaaat tgatcaacta cgacattctg ctacaagtgc attatttgaa
     6961 cgcgatgatg taattattat tgctagtgta agttgtatat atggtttagg taatcctgaa
     7021 gaatataaag atttagtagt aagtgttcga gttggtatgg aaatggatag aagtgaatta
     7081 cttagaaaac ttgtagatgt gcaatataca cgaaatgaca tcgatttcca acgaggaacg
     7141 tttcgagtgc gtggtgatgt agtggaaata ttcccagcct ctaaagaaga actttgtata
     7201 agggttgagt ttttcggcga tgagattgac cgtatccgag aagttaacta cctaacaggt
     7261 gaagtgttga aagaaagaga acattttgcg atattcccag cttctcactt cgtaacacgt
     7321 gaagaaaagt tgaaagttgc gattgaacgt attgaaaaag aattggaaga acgattgaaa
     7381 gaattacgag atgagaataa attactagaa gcgcaaaggt tagaacagcg taccaactat
     7441 gatttagaaa tgatgcgaga gatgggattc tgttcaggaa ttgaaaacta ttccgtacat
     7501 ttaactttgc gaccactggg ttcgacacca tatactttat tggattactt tggcgatgat
     7561 tggttagtaa tgattgatga atcacatgtg acattaccgc aagttcgagg catgtataac
     7621 ggagacagag cgcgtaaaca agttttggtg gatcatgggt ttagattacc gagtgcatta
     7681 gataaccgtc cacttaaatt tgaagaattt gaagaaaaga caaaacaact tgtgtatgta
     7741 tctgcaacgc ctggaccata cgaaattgaa catacggata agatggttga acaaattatt
     7801 cgtcctactg gtttactgga tcctaagatt gaggttagac ctactgaaaa tcaaattgac
     7861 gatttattaa gtgaaattca aacaagagtt gagcgtaatg aacgcgtact tgttacaacg
     7921 ctcactaaaa agatgagtga agatttaacc acatacatga aagaagcggg tattaaagtt
     7981 aattatctgc attcagaaat caagacatta gaacgaattg aaataattag agacttacga
     8041 atgggtacat atgatgttat cgtaggtatt aatttattaa gagagggtat tgatatacca
     8101 gaagtttctc tagttgtcat attagatgca gataaagaag ggtttttacg ttctaaccgc
     8161 tcattaattc aaacaatagg tagagctgcg cgtaacgata aaggtgaagt cattatgtat
     8221 gccgataaaa tgactgattc gatgaagtat gcaattgatg agacacaacg tcgtcgagaa
     8281 atacagatga aacataatga aaaacatggt attacaccta aaacaattaa taaaaaaata
     8341 catgatttaa ttagtgctac tgttgaaaat gacgaaaata atgacaaagc acaaactgtg
     8401 atacctaaga agatgacgaa aaaagaacgt caaaagacaa tcgacaatat agaaaaagaa
     8461 atgaaacaag cagcgaaaga tttagatttc gagaaagcta cagaattaag agatatgtta
     8521 tttgaattaa aagcagaagg gtgacaagta aatgaaagaa ccatccatag tagtaaaagg
     8581 tgctcgtgcg cataacttga aagatattga tatcgaacta cctaaaaata aattaattgt
     8641 tatgacaggt ttatctgggt caggtaaatc gtcattagca ttcgatacta tatatgctga
     8701 aggacaacga cgttatgttg aatcattaag tgcctatgcg cgtcaatttt taggccaaat
     8761 ggacaaacca gatgttgata caattgaagg attatcgcca gcaatttcaa tagatcaaaa
     8821 aacaacaagt aaaaatccaa gatcaactgt agcaacagta acagaaatat atgattatat
     8881 acgtttgtta tatgcacgtg ttggtaaacc ttactgtcca aatcacaata tagaaattga
     8941 atcgcaaaca gtacaacaaa tggttgaccg cattatggaa ttagaggcac gtacaaagat
     9001 tcaattatta gcacctgtca tcgctcatcg taaaggtagt catgaaaagc taatcgaaga
     9061 tattggtaaa aaaggttatg tacgtttaag aatcgatggc gaaattgttg atgtaaatga
     9121 tgtacctact ttagataaga acaagaatca tacaatagaa gttgttgtag accgattagt
     9181 tgttaaagat ggaattgaaa cacgactagc tgactctata gaaactgcct tagagctttc
     9241 agaaggacaa ttaacagtcg atgtcattga cggggaagac cttaagtttt cagaaagcca
     9301 tgcttgtcct atatgtggat tttcaatcgg agagttagaa ccaagaatgt ttagctttaa
     9361 cagtcctttt ggtgcttgtc cgacatgtga tggcttaggc caaaagttaa cagtcgatgt
     9421 agacttggtt gttcccgaca aagataagac gctaaacgaa ggtgcaatag aaccttggat
     9481 accgacgagt tctgattttt atccaacatt gttaaaacgt gtttgtgaag tttataaaat
     9541 caatatggat aaacctttta aaaagttaac agaacgtcaa cgtgatattt tattgtatgg
     9601 ttctggtgac aaagaaattg aatttacatt tacacaacgt caaggtggta ctagaaaacg
     9661 aacaatggtt ttcgagggtg tagttcctaa tataagtaga cgattccatg aatctccttc
     9721 agaatataca cgtgaaatga tgagtaaata tatgactgaa ctaccttgcg aaacttgtca
     9781 tggaaagcga ttgagtcgtg aagcgttatc tgtttatgta ggtggtttaa atattggtga
     9841 agtagtcgaa tattcaatca gtcaagcgct gaactattat aaaaacattg atttgtcaga
     9901 acatgatcaa gcgattgcaa atcaaatatt gaaagaaatt atttcccgac tcactttttt
     9961 aaataatgtg ggacttgaat atttaacgtt aaacagagct tcaggtacac tttcaggtgg
    10021 tgaagcacaa cgtattcgat tagcaacgca aattgggtcg cgtttgactg gtgtcttata
    10081 tgtattagat gagccatcaa ttggactgca tcaaagagat aatgatcgat taattaatac
    10141 acttaaagaa atgagagatt taggaaatac tttaattgta gttgaacacg atgatgatac
    10201 aatgcgtgcg gctgattact tagtggatat aggtcctggt gctggtgaac atggagggca
    10261 gattgtgtct agtggtactc ctcaaaaggt aatgaaagat aaaaaatcat taacaggaca
    10321 atacttgagt ggtaagaaac gtattgaagt acctgaatat cgcagaccgg cttcagatcg
    10381 taaaatttct atacgtggag ctagaagcaa caatcttaaa ggggttgatg tggacatacc
    10441 actatcaatc atgacggttg ttacaggtgt atcaggttct ggtaaaagct cattagtaaa
    10501 tgaagtatta tacaaatcat tagctcaaaa aattaataaa tctaaagtaa agccaggatt
    10561 gtacgataag attgaaggta ttgatcaact tgataaaatt attgatattg atcaatcacc
    10621 aataggtaga acgccacgct ctaatccagc aacatatact ggtgtgtttg atgatatacg
    10681 tgatgtgttt gcgcaaacaa atgaagctaa aattcgagga tatcaaaaag ggcgttttag
    10741 ttttaatgta aaaggtggac gctgtgaagc ttgtaaaggt gacggtatta ttaaaattga
    10801 aatgcatttt ttacctgatg tttatgttcc ttgtgaagtg tgtgatggta aacgatataa
    10861 tcgtgagaca ctagaggtta cttacaaagg taaaaatatt gctgacattt tagaaatgac
    10921 tgttgaagaa gcaacacaat tttttgaaaa tattcctaag attaagcgca agttacaaac
    10981 actagttgat gttggtcttg gatacgtcac attaggtcaa caagctacaa cgttatcagg
    11041 tggtgaggct caacgtgtga aacttgcatc tgaacttcat aaacgttcaa ctggtaaatc
    11101 tatttatatc ctagatgaac cgacaacagg gttacatgtt gacgatatta gtagattatt
    11161 aaaagtatta aaccgattag ttgaaaatgg tgatactgtt gtaattattg aacataacct
    11221 agatgttatc aaaacagcag actatattat agacttaggt cctgaaggtg gtagtggcgg
    11281 tggtactatt gttgcgactg gcacacccga agatattgct cagacaaagt catcatatac
    11341 aggaaagtat ttaaaagaag tacttgaacg agataaacaa aatactgaag ataaataaga
    11401 ttaaaagaag tgaaggatgt tataaattta tccttcgctt ctttttatta atttagtaat
    11461 gaatagtaga aagaaaagat gcgtaaaaag aattatgtta agatagggtc aatctagagt
    11521 agttaaacat aaatcgaact gggagtggga cagaaatgat aaagaatcac taatgattta
    11581 ttatgtagtg gttctttgtc attagccaca gctattgtgt acttaaaaat aggaatgcat
    11641 gagtgcaact catgcataag aaatactaat ttctaaagaa aaagtatttc tttatgttgg
    11701 ggccccgcca acttgcattg tttgtagaat ttcttttcga aattctttat gttggggccc
    11761 cgccaacttg cattgtttgt agaatttctt ttcgaaattc tttatgttgg ggccccgcca
    11821 actaattcca atatatcatt gtagagctta ggtcattgat ttttggctcg gacttttatg
    11881 gcgatatgaa ccatgtaaat taagcaagca ataaattaat gattgatatt gacttgtaaa
    11941 ataataacaa taatgaacaa ttaatattta ttttagcttt tcaatgtaga ttggtgttat
    12001 atttttgata tgataagaag agatgtaaga gtagggataa atacaattga ggtgaaccca
    12061 tgttaacgac agaaaaacta gttgaaacat taaagttaga tttaatcgct ggtgaagaag
    12121 gactatcgaa gccaattaaa aatgctgata tatcaagacc gggcttagag atggcaggtt
    12181 atttttcaca ttatgcgtca gatagaatac aactattagg aacaacggaa ctatcgtttt
    12241 acaatttatt accagataag gatcgcgcag gtcgtatgcg taaactatgc agaccagaaa
    12301 cgcctgcaat tattgtgaca cgtggattgc agccaccaga agaattagtt gaagctgcaa
    12361 aagaattaaa taccccactt atagttgcta aagatgcgac tacaagttta atgagtcgct
    12421 taacaacgtt tttagagcat gcacttgcaa agacgacatc tttacatggt gttttagtag
    12481 atgtttacgg tgttggtgta ctaattaccg gtgattcagg aataggtaaa agtgagactg
    12541 cgttggaatt agttaaacgt gggcatagat tagtagcaga tgataatgta gaaatacgtc
    12601 aaattaataa agatgaacta atagggaaac caccaaagtt aatagaacat ctattagaaa
    12661 tacgtggact aggtattatc aatgttatga ctttatttgg cgcgggttca atattaactg
    12721 aaaaacgaat tagattaaat attaatttgg aaaactggaa caagcaaaag ttatatgacc
    12781 gcgtaggtct taatgaagag acgctaagta ttttagatac tgaaatcact aaaaaaacaa
    12841 tacctgtaag acctggtaga aatgttgcgg taattattga ggtcgctgca atgaactatc
    12901 gattaaatat catgggcatt aacacggccg aagaatttag tgaaagatta aatgaagaaa
    12961 ttatcaagaa cagtcataag agtgaggagt aggttgaatg ggtattgtat ttaactatat
    13021 agatcctgtg gcatttaact taggaccact gagtgtacga tggtatggaa ttatcattgc
    13081 tgtcggaata ttacttggtt actttgttgc acaacgtgca ctagttaaag caggattaca
    13141 taaagatact ttagtagata ttatttttta tagtgcacta tttggattta tcgcggcacg
    13201 aatctatttt gtgattttcc aatggccata ttacgcggaa aatccaagtg aaattattaa
    13261 aatatggcat ggtggaatag caatacatgg tggtttaata ggtggcttta ttgctggtgt
    13321 tattgtatgt aaagtgaaaa atttaaaccc atttcaaatt ggtgatatcg ttgcgccaag
    13381 tataatttta gcgcaaggaa ttggacgctg gggtaacttt atgaatcacg aggcacatgg
    13441 tggatcggtg tcacgcgctt ttttagaaca attacatttg cctaatttta taatagaaaa
    13501 tatgtatatt aacggccaat attatcatcc aacattctta tatgaatcca tttgggatgt
    13561 cgctggattt attatcttag ttaatattcg taaacattta aaattaggag aaacattctt
    13621 tttatattta acttggtatt caattggtcg attctttata gaaggatta
//
LOCUS       DAKTIP010000058        13317 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAKTIP010000058 DAKTIP010000000
VERSION     DAKTIP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.058"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            106..558
                     /gene="argR"
                     /locus_tag="O0305_001266"
     CDS             106..558
                     /gene="argR"
                     /locus_tag="O0305_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCY0274415.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            574..2253
                     /gene="recN"
                     /locus_tag="O0305_001267"
     CDS             574..2253
                     /gene="recN"
                     /locus_tag="O0305_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCY0274416.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            2404..3825
                     /gene="lpdA"
                     /locus_tag="O0305_001268"
     CDS             2404..3825
                     /gene="lpdA"
                     /locus_tag="O0305_001268"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCY0274417.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDTNEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3841..4833
                     /locus_tag="O0305_001269"
     CDS             3841..4833
                     /locus_tag="O0305_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCY0274418.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4833..5816
                     /locus_tag="O0305_001270"
     CDS             4833..5816
                     /locus_tag="O0305_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCY0274419.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5829..7103
                     /locus_tag="O0305_001271"
     CDS             5829..7103
                     /locus_tag="O0305_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCY0274420.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIIKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7454..7891
                     /locus_tag="O0305_001272"
     CDS             7454..7891
                     /locus_tag="O0305_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCY0274421.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7905..8885
                     /locus_tag="O0305_001273"
     CDS             7905..8885
                     /locus_tag="O0305_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0274422.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEVLLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(9012..9191)
                     /gene="prli42"
                     /locus_tag="O0305_001274"
     CDS             complement(9012..9191)
                     /gene="prli42"
                     /locus_tag="O0305_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCY0274423.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            9454..10587
                     /locus_tag="O0305_001275"
     CDS             9454..10587
                     /locus_tag="O0305_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCY0274424.1"
                     /translation="MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK"
     gene            10655..12061
                     /gene="gndA"
                     /locus_tag="O0305_001276"
     CDS             10655..12061
                     /gene="gndA"
                     /locus_tag="O0305_001276"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCY0274425.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            12280..12651
                     /locus_tag="O0305_001277"
     CDS             12280..12651
                     /locus_tag="O0305_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0274426.1"
                     /translation="MEKLNQVMLYVDDVEKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKHSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            12590..12844
                     /locus_tag="O0305_001278"
     CDS             12590..12844
                     /locus_tag="O0305_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274427.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            12845..>13317
                     /locus_tag="O0305_001279"
     CDS             12845..>13317
                     /locus_tag="O0305_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0274428.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDID"
BASE COUNT         4857 a         1825 c         2568 g         4067 t
ORIGIN      
        1 tagataaacc atgtttaccc tttttacgta aaggtgttta gtagtcgtaa atcagtgcat
       61 gtgttaaaat aaatgtataa atattcgtaa tcagaggtgt ttactgtgcc caaaaaatcg
      121 gttaggcata taaaaattag agaaattatt tcaaatgaac agatagagac acaagatgaa
      181 ttagttaaac gattaaacga ttatgattta aatgtcactc aagcaactgt ttctcgtgat
      241 attaaagaac tacaacttat taaagtacct ataccttcag gtcaatatgt ttatagttta
      301 ccaaatgata gaaaattcca tcctttagaa aaattgggac gttatttaat ggattccttt
      361 gttaatatag atggtactga taatttactt gttctaaaaa cattacctgg taatgcacaa
      421 tctattggag ctatattaga ccaaatcaat tgggaagaag tactaggcac aatttgtggt
      481 gatgatactt gtttaattat ttgtcgaagc aaagaggcaa gtgatgaaat caagtcaaga
      541 attttcaatt tgttataagg atgcgatgag tttatgttac aaaccttatc aatcaagcaa
      601 tttgctatta ttgaagaatt agaaattcaa ttttctgatg gtttaactgt tttaagtggt
      661 gaaacgggtt caggtaaatc aattattata gatgcaattg gacaattaat tggaatgaga
      721 gcttcatctg attttgtaag acatggtgaa aaaaaggctg tcatcgaagg tatatttgat
      781 attgacgaga gtaaagatgc tatacatata ttgaaaaata tggatattga tgtagatgaa
      841 gattttttat tagttaaacg ggaaattttc agctctggta aaagtctttg taaaattaat
      901 aatcaaacag ttactttaca agatttacga aaagttatgc aagaattatt agatattcat
      961 ggccaacatg aaacgcagtc tttgttgaaa caaaaatacc atttaacatt attggataat
     1021 tacgcagaat cacgttatca agatttgttg gataaatatc accaaacttt tcaaaattat
     1081 aaagccaaaa agcaagagtt agaagattta gaatcagcag accaagcatt gctacagcgt
     1141 ttagatttaa tgaaattcca attagaagaa ttgtctgaag cacatctaaa agaaggcgaa
     1201 atagagcaac ttgaaataga tattaaacgc attcaaaact ctgaaaaatt aagcttagcg
     1261 cttaataacg cacatatgac attaacggat gaaaatgcta tcactgatcg tttatatgaa
     1321 ctaagcaacc atttgttaac aataaacgat atagttccaa acaaatatga caaattaaaa
     1381 gaagatattg atcaatttta ctacatttta gaagatgcaa aacatgagtt atatgatgaa
     1441 atggctaata cagaattcga cgaacaagtg ttaaacgagt atgaatctcg tatgaatttg
     1501 ctgaataatt taaaacgtaa atatggaaaa gatatttcag aattaatcgc atatcaagaa
     1561 aagcttaata atgaaatcaa taaaattgaa aactatgaac aaagtacatc gcagttacga
     1621 gaagaaataa atgcattgta taatcaagtt atagaggttg gacaagcgtt atcaaagcag
     1681 cgtcgtatag tcgctaggga gttaagagac cacattgtat cggaaattca aaacttacaa
     1741 atgaaagacg caaatcttga gatttcattt aaaaaattag aagaaccgaa tattgatgga
     1801 atcgaatttg tagaattttt aatcagtcca aataaagggg aaccattaaa aagtttaaat
     1861 aaaattgcgt caggtggaga actttctaga attatgttag cgttaaaaag tatttttgtt
     1921 aaatcgagag gtcaaactgc aattttattc gatgaggttg actcaggtgt atctggacaa
     1981 gctgcacaaa aaatggcaga gaaaatgcga gatatagcag aatatataca agttatttgt
     2041 atctctcact taccacaagt tgcatcgatg agcgaccatc atttattaat ttcgaaatca
     2101 tcaaaagatg atcgtacaac aacacaagtt caagagttaa ttagcgatga taaagtagat
     2161 gaaattgcac gaatgatttc aggtgcaagt gttactgatt taactcgaga aaatgcaaga
     2221 gaaatgatac aacacaatca aagacgtaga taatttccaa agtgtgttga atactcattt
     2281 atggatttat ttatgtgcat tcaaactaaa tagacaatca tgtaaaatag gtaattaatg
     2341 gacatgaaaa ctatgtatta ataaattttc aatggatata tatcattagt taggagtaag
     2401 aacatgtcag agaaacaata tgatttagtc gttctcggtg gaggtactgc aggatatgtt
     2461 gctgcgatac gtgcgtctca attaggtaaa aaagtggcaa tagtggaacg acaactatta
     2521 ggaggtactt gtctccataa aggttgtatt cctactaaat cgctgcttaa atctgctgaa
     2581 gtatttcaaa cagtaaaaca ggcagcaatg tttggcgttg atgtcaaaga tgctaatgtt
     2641 aattttgaaa atatgttggc acgaaaagaa gacattatta atcaaatgta tcaaggtgta
     2701 aagcatttaa tgcaacacaa tcacattgac atttataatg gcacgggacg tattctaggt
     2761 acatctatat tttcacctca aagtggcaca atttctgtcg aatatgaaga tggtgaatca
     2821 gacttattac ccaaccaatt tgtattaatt gcaactggtt cttcgcctgc agagttgcca
     2881 ttcttatcat ttgatcatga taaaatttta tctagcgatg atatattatc attaaagacg
     2941 ttaccatcaa gtattggtat tattggtggt ggtgttatcg gaatggaatt tgcatcattg
     3001 atgatagatt taggtgttga cgtaacagtt atagaagcag gtgaaagaat tttaccaact
     3061 gaaagtaaac aagcttcaca actattaaaa aaatcattgt cagcacgtgg tgttaaattc
     3121 tatgagggaa taaaactttc tgaaaatgat ataaatgtta acgaagatgg tgttacgttt
     3181 gaaatttcct cagacataat taaagtagat aaagtgttgc tatcgattgg tagaaaacct
     3241 aacacatcag atattggttt aaacaacact aaaataaaac tttcaacatc aggacatatt
     3301 ttaacgaacg aatttcaaca aactgaagat aaacatattt atgcagctgg tgattgtata
     3361 ggaaaattac aattggcaca tgttggatca aaagaaggtg ttgtggctgt tgatcatatg
     3421 tttgagggga acccaatccc agtaaactat aacatgatgc ctaagtgtat ttattcacaa
     3481 cctgaaattg cttctatcgg tttaaacatt gaacaagcaa aggcagaggg aatgaaagtt
     3541 aaaagtttta aagtaccatt taaagcaatt ggtaaagcag tgattgatag ccatgacaca
     3601 aacgaagggt atagcgaaat ggtgattgat caatcaactg aagaaattgt gggtattaat
     3661 atgattggtc cacatgtaac agaattgatt aatgaggcat cactgttaca gttcatgaat
     3721 ggctcggcat tagaattagg actaacaaca cacgcacatc cttccatctc tgaagtgttg
     3781 atggaattag gattgaaagc agaaagtaga gctattcacg tataaatagg aggatttaac
     3841 atgattgatt ataaatcatt aggccttagc gaagaagacc taaaagtaat atataaatgg
     3901 atggatttag gtagaaaaat agatgaaaga ctgtggttat tgaaccgtgc aggtaaaatt
     3961 ccatttgttg taagtggtca aggacaagaa gcaactcaga ttgggatggc atacgcatta
     4021 gaagaaggtg atattacagc gccttattat agagatctag ctttcgttac ttatatgggt
     4081 atctcagcgt atgatacgtt tttatcagcg tttggaaaga aagacgatgt taactctggt
     4141 ggaaaacaaa tgccatcaca ctttagtagt agagctaaga atattttgtc tcaaagttca
     4201 ccggtagcaa ctcaaatacc ccatgctgtt ggtgctgctt tagctttaaa aatggatggt
     4261 aagaagaaaa ttgcaacagc aactgttggt gaaggtagct caaaccaagg tgacttccat
     4321 gaaggtttaa acttcgcggg tgtacacaaa cttccttttg tatgtgtcat tataaataat
     4381 aaatatgcaa tttcagtacc tgattcatta caatacgcag ctgaaaaatt atcggataga
     4441 gcacttggtt acggtataca tggtgaacaa gtagatggta atgatccatt agcaatgtat
     4501 aaagcgatga aagaagcaag agatcgagct atttctggtc aaggttcaac tttgattgaa
     4561 gcagtaacaa gtcgtatgac agctcattca tcagatgatg acgaccaata tcgtacgaaa
     4621 gaagagcgtg aaacgcttaa aaaagcagac tgcaatgaaa agttcaaaaa agagttgctt
     4681 tcagctggca ttatcgatga tgcttggttg gcagaaatag aagcggagca taaagacatc
     4741 atcaataaag cgacaaaggc tgctgaagac gcgccatatc caagtgtcga agaagcctat
     4801 gcatttgtgt acgaagaagg gagtcttaac taatggctaa attatcttat ttagaggcga
     4861 tacgccaggc acaagactta gcattacaac aaaataaaga tgtgtttatt cttggtgaag
     4921 atgttggtag aaaaggtggc gtatttggta ctactcaagg actgcaacaa aaatatggtg
     4981 aagacagagt tatcgatacg ccattagctg agtcaaatat tgttggaact gccattggtg
     5041 cagctatggt aggtaaaaga ccaattgcag aaattcaatt tgcagatttt attttacctg
     5101 cgacaaatca aattattagt gaagctgcga aaatgcgtta tcgttcaaat aatgattggc
     5161 aatgcccatt aacgattcgt gcaccatttg gtggcggtgt tcacggagga ctatatcatt
     5221 cacaaagtat tgaaagtata tttgcttcat cgccaggttt aacaattgtt attccttcca
     5281 caccgtatga tgctaaaggg cttttattat catctatcga atcaaatgat ccagtattgt
     5341 actttgagca taaaaaagct tatcgtttct taaaggaaga agtgccagaa gaatattaca
     5401 ctgtaccttt aggaaaagca gatgtgaaac gtgaaggtga agatttaaca gtattttgtt
     5461 atggattaat ggtcaattat tgtttacaag cagcggatat tttagctgca gacggtatca
     5521 atgttgaagt cgttgattta cgaactgttt atccattaga taaagaaaca attattgatc
     5581 gtgctaaaca tacaggtaaa gttttacttg taactgaaga taatttagaa ggtagtatta
     5641 tgtcagaagt gtcagcgatt attgcagagc attgcttgtt cgatttagat gcaccaatca
     5701 tgcgtttagc tgctccagat gtaccatcta tgccattttc tcctgtatta gaaaatgaaa
     5761 ttatgatgaa tccagaaaaa atcttaaata aaatgcgtga attagcagaa ttctagggag
     5821 ggaaagtcat ggaaataaca atgcctaagt taggtgagag tgttcatgaa ggcaccattg
     5881 aacaatggtt agtttctgtt ggtgatcata ttgatgaata tgaaccatta tgtgaagtta
     5941 ttacagataa agtgacagct gaagtccctt ccacgatatc aggaacaatt acagaaattt
     6001 tagttgaagc ggggcagaca gtagctattg atacaattat ctgtaaaatt gaaactgctg
     6061 atgaaaagac aaatgaaaca actgaagaga tacaagcaaa agtggatgag catactcaga
     6121 aatctactaa aaaagctagt gcaacagtgg aacagacatc tactgctaaa caaaatcaac
     6181 cacgtaataa tggtcgcttt tcacctgttg tatttaaact cgcttcagag catgacattg
     6241 atttatcaca agttgtaggt agtggatttg aaggtcgtgt aactaagaag gatataatgt
     6301 cagttattga aaatggtggt accacagctc aatctgacaa acaagttcaa acaaaatcaa
     6361 catcagtaga tacatcaagt aaccaatcat ctgaagacaa tagtgaaaac agcacaatac
     6421 cagtaaatgg tgtgcgtaaa gcaattgcgc aaaatatggt taatagtgta acagagattc
     6481 cacatgcatg gatgatgatt gaagtagatg ctacaaatct tgtgaatacg agaaatcatt
     6541 ataaaaacag ctttaaaaat aaagaaggat ataatctaac gttctttgct ttctttgtaa
     6601 aagctgtagc agatgcttta aaagcatatc ctttattaaa tagtagctgg caaggaaatg
     6661 aaattgtctt acataaagac attaatattt caattgctgt tgctgatgaa aataaattat
     6721 acgtacctgt gattaagcat gcagacgaaa agtcaatcaa aggtatagct agagaaatta
     6781 atactttagc aacgaaagcg cgtaataagc aattgacagc tgaagatatg cagggcggta
     6841 catttacggt aaataatact ggtacatttg gttcagtatc atcaatgggt attataaatc
     6901 atccacaagc agcgatttta caagtagaat caatcattaa aaagccagta gtaattaatg
     6961 atatgattgc aattcgtaac atggttaatt tatgtatttc aattgatcat cgtattttag
     7021 atggtttaca aacaggtaaa tttatgaatc atattaaaca gcgtatcgaa cagtatactt
     7081 tagaaaatac aaatatatat tagtgataac atagatgcat ctatcgacaa cttgttttat
     7141 cttgttcttg tcgatggatg tattattttt tggcactaaa atatgtgcaa tatattcaaa
     7201 aaagataaag aacaataatc aacatggttg aatgcatttt tgcagtaaag tcaaaataag
     7261 acatcatact tgaaacatat taatgaaaac atgtgaacaa attagttacc atgattttaa
     7321 gcacaataat gtttggtata ttgttaaaat tgtgtctaaa tataggtgtg attcagatta
     7381 gtttattgaa caatatgtta ttaattagta gaatgaggat agtttaaata taaagggata
     7441 ggtgattgaa cttatggaca tgaatttcga tttatacatg aacggtgttg tagaacaagc
     7501 aaggaatgaa attgaatctg cgggatatga gcaattaact actgcagaag atgttgacaa
     7561 agttcttaaa caagatggta caacactagt tatgatcaat tctgtatgtg gttgtgcagg
     7621 tggtatcgca agaccagcag catcacatgc tttacattat gacgtattac ctgatcgtct
     7681 agtgacagta tttgctggac aagataaaga agcgacacaa agagcgcgtg aatacttcga
     7741 aggttatgcg ccttcaagtc cgtcatttgc attagtaaaa gatggaaaga ttacagaaat
     7801 gattgaaaga catcaaatcg aaggtcatga tgtgatgaac gtaattaatc aattacaaac
     7861 attattcaat aaatattgtg aagaaagata agaggcgcta acccatgtta aagttaaatc
     7921 cttacaagat tggatttaga acaataaaaa cagcagtggg tatgacttta ggtgtaatta
     7981 ttagtaagct gttaggttta gataattatg cttcaagcgc catattagtc gtattatgta
     8041 ttaaacatac aaaagtacat tcgctacaag cgattatttc aagattagta tcatgttttt
     8101 tagtattgtt tttaggttca gcaatattta gtttattagg tcagagtcca attgtactcg
     8161 gtattatcgt attgttattt ataccattaa ctgtcgtatt aaaagtacaa gaaggtgtca
     8221 ttacgagttg cgttatatta cttcatgttt ttaatgcaaa atcaattgat gcacatttaa
     8281 ttgttaatga aacattatta ctgttaattg gactaagcat tgcatttaca atgaatttaa
     8341 tgatgccaag tttagacaaa caactagacg aatacaaatg taaaattgag caacaaattg
     8401 ctgatatttt tagtaaatat agttatattt gtgaaaaata tgaagatacc attgcgattg
     8461 aatttgaagt gttactttta aatattaaaa aggcgaagtc tatcgcgttc cgagatgtta
     8521 aaaatcattt tgttagaaac gaaaattcat actatcatta ttttgatatg cgagaagagc
     8581 aagtggaatt gttaatgaga atgaaaccgc tcatcgaaag tatctgtcat aaagatccgc
     8641 ttttaaatga tgttgcgtca ctgctgaaag aaattggtct aaatgtgaat agtaatgact
     8701 atacagcaat gcgtttacac aatttgtatg agacgcgttt gaaattggat gaactgccat
     8761 tgccaacaac acacaaagcg cttaactcga gagcaagtat tattcaaata ttaaatgaat
     8821 tagaagaata tttacaaatc aaatcgcaat ttggttcatt gaaaatgcat agtgaagtag
     8881 cttaaaaaat aatccatccg tatgagccat gtttgaaagt aatcgttgca atacgataac
     8941 ttattaatac atgcgtttat aatggatgga tttttaattg tatgattttg tataggtagt
     9001 tgagatatag tttataaaat tggaatgata ctcgttaata acaatgctaa tacaactgat
     9061 acaagcataa cgataatgat aattcttaaa actttatttt gcattttgtg tgcctccaaa
     9121 tgaataacct ttgtattaat aattacgaca taagatataa aacttttaac ttgagacgat
     9181 ataactagca atcatcataa aggaatataa taaaaaacat gagtaagttt gcttatgtca
     9241 ttttcctcat tatacgatat ctttgaaaaa tcaatcaata tatttttgac aaagcttagt
     9301 ttgagttaca ataattatat ataataaatt gaaaataata tgacacaatt tacaaacctg
     9361 ataaatctgt gtaacgtcaa ttactgtaac attttatttc atcaattttt accgtttggc
     9421 gtacatgaaa attttgataa aaggggttcc attatgatta atgagcaaag attacttaat
     9481 acgttttttg aacttgtaca aatcgattct gagacaggga atgaatcaac aatccaacct
     9541 attttaaaag aaaaattcat agcattaggg ttagatgtta aagaagatga agcggctaaa
     9601 catcctaaat taggtgccaa taatctagtg tgtacaatga atagtactat cgaagaaggc
     9661 gaagtaccta aattatattt gacgagccat atggatactg ttgtacccgc aattaatgta
     9721 aagccaattg taaaagatga cggctacata tactctgatg gtacgactat tctaggtgca
     9781 gatgataaag caggattagc agcgatgctt gaagttttgc aagtgataaa ggaacaacaa
     9841 atcccacatg gacaaattca atttgtgatt actgttggag aagaatcagg tttaattggt
     9901 gctaaagaat taaattcaga gttgttggac gcggactttg gttatgctat tgatgcaagt
     9961 gctgatgtcg gtactacggt tgtaggtgca ccgacgcaaa tgttaatttc agctaaaatt
    10021 tttggcaaaa cggctcatgc aagtacgcca aaagagggtg ttagtgctat taatattgcg
    10081 gcgaaagcaa ttagtcgaat gaaattagga caagttgatg aaataactac tgccaatatt
    10141 ggtaagtttc atggcggttc agctacgaat attgttgcag atgaagtcat tttagaagca
    10201 gaggcacgtt ctcacgatcc agaaagaata aaaacacaag tcaaacatat gactgatgtg
    10261 tttgaaacta ctgcatcaga attaggtggg aaagcagtag ttacagtgga gcaaagttat
    10321 ccaggattta aaattaatga taatgaagcg gttgttaaaa ttgcacaaga aagtgctcgt
    10381 aatttaggat tatctgcaaa tacaattatt tctggtggcg gttcagacgg cagtattatt
    10441 aatacatttg gtattccttc agtcatttta ggagtgggtt atgaaaaaat ccatacaaca
    10501 aatgagagaa tgccgattaa atcattgaac ttgcttgcaa gtcaagtgtt agaaataatc
    10561 aaaatagttg cgcgacattc gaaatgattt agtgtagtgt atgtctattt tgtgatacaa
    10621 tactaatgaa aaatcaacaa gtgaggtaag taatatgaca caacaaattg gagttattgg
    10681 tttagccgtt atgggtaaaa acctagcttg gaatattgaa tcacgcggat atagtgtatc
    10741 tgtatttaac cgctcaagtg aaaaaactga tttaatggtt gaagaatcaa aagggaaaaa
    10801 tattcatcca acgtattcat tggaagaatt tgttaattct ttagaaaaac cacgtaaaat
    10861 tttattaatg gttcaagcag gtaaagctac ggacgcaacg attgatagtt tgttaccttt
    10921 attagatgat ggagatattt taattgatgg tggtaataca aactatcaag atactatcag
    10981 acgtaataaa gcattagcac aaagtgcaat caactttatt ggtatgggcg tttctggtgg
    11041 tgaaattgga gcgttaactg gtccgtcatt aatgccgggt ggacaagaag aagcttataa
    11101 caaagttgca gatattcttg atgcaattgc tgcaaaggca aaagacggtg cttcatgtgt
    11161 tacttatatt ggacctaatg gtgctggaca ctatgtgaaa atggtacaca atggtatcga
    11221 atatgctgac atgcaattaa ttgcagaaag ttatgctatg atgaaagaat tattaggcat
    11281 gtctcatgaa gacattgctc aaacgttcaa agactggaat gctggtgaat tagaaagtta
    11341 tttaattgaa atcactggtg atattttcat gaaattagat gaaaataaag aagcattagt
    11401 tgaaaaaatt cttgatactg caggtcaaaa aggtacagga aaatggactt caattaacgc
    11461 attagaatta ggtattccat taacaatcat tactgaatct gtatttgcac gttttatctc
    11521 ttcaatcaaa gaagagcgcg taaatgcttc taaagaatta aatggtccaa aagcatcatt
    11581 tgatggtgac aagaaagact ttttagaaaa aattcgtaaa gcattatata tgagtaaaat
    11641 ttgttcttat gcacaaggtt tcgcgcaaat gcgtaaagca agtgaagata atgaatggaa
    11701 tttaaaactt ggtgatttag ctatgatttg gagagaaggt tgcattatcc gtgcacaatt
    11761 cttacaaaag atcaaagatg cttacgataa taatccagga ttacaaaact tattgctaga
    11821 tccttacttt aaaaacatcg ttactgaata tcaagatgcg ttacgtgatg ttgtcgcaac
    11881 tggtgttcaa aatggtgtac caacacctgg tttctcatca agtattaatt actatgatag
    11941 ttaccgtgct gctgatttac ctgcaaactt aattcaagca caacgtgact acttcggtgc
    12001 tcatacgtac gaaagaaaag ataaagaagg cgtattccat acacaatgga ttgaagaata
    12061 atattgatta aaggatagtt gaaagatact atcctttttt atatcttttg ataaatataa
    12121 aagagagtgt gatagagtag ctacaaccta taaaaaatta cacaagtatt atccttgacc
    12181 ttaaaagcat cccgccatat ttttaagcat tttgtgaagt aataaaatat ttactgtgtt
    12241 atgatagcat gtactataga gaaatagagg tactatttta tggaaaagtt aaatcaagtt
    12301 atgttatatg ttgatgatgt cgaaaaagcg aaagcatttt ggacagaaac tttagaattt
    12361 gttgttgtaa gtgaaacacc gttagctgag gactatgttg cagttgaagt atcacctacc
    12421 aaagatgctg aaacgtcatt gacgattatg gcaaaagaat ttattgaaaa gcatagcccg
    12481 gaagtgaacc taggaacacc atctttaatg tttaaagaga aaaactttga tgcattatac
    12541 tcaaaattaa atgatttagg tttaactggg catgatatag tagaaatgaa tggtcaacgt
    12601 gtatttaatt ttcaagatgg ccaaggtaac tattttgcag ttagtgacta aacaatcgat
    12661 tttaatattt atacaaacct ttatattgat tttcggattg aatttcagta tgaaggtttt
    12721 atttttatcg ttttatgcaa tcgtttgcac aaatcttttg acttttttag aaagtgtgac
    12781 taaactggta ttatacagtg gttggaagcg ttttccaatg cagaaattaa caggaggtga
    12841 ttaaatggtt acgattaaag atgttgcact aaaagccggt gtttctcctt cgacagtttc
    12901 aagagttata aaaggaaatc aacgtattag cgaagcgaca atttcaaaag tgaagaaagt
    12961 tatggaagaa ttgaattatt ttcctaatac cgctgctaga actttaatta caaaccaaac
    13021 atataaaatt ggtttagtgt taaaagggtc tgaggagcct attcgactga atccattcta
    13081 catcaatgta ttgctaggaa tttctgaaac gtgtaaccag catggctatg gtacacaaac
    13141 gacagtctca aataatatga atgatttaat ggatgaagtt tataaaatga ttaaacaacg
    13201 aatggttgat gcgtttatac tgctctattc aaaagaaaat gatccgatta aacaaatgtt
    13261 aattgatgaa agcatgccat ttattgtgat tggtaagcct acatcggata tagatca
//
LOCUS       DAKTIP010000059        13142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAKTIP010000059 DAKTIP010000000
VERSION     DAKTIP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.059"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(40..642)
                     /gene="rpsD"
                     /locus_tag="O0305_001280"
     CDS             complement(40..642)
                     /gene="rpsD"
                     /locus_tag="O0305_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCY0274429.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(886..1350)
                     /locus_tag="O0305_001281"
     CDS             complement(886..1350)
                     /locus_tag="O0305_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCY0274430.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            1487..3181
                     /gene="ezrA"
                     /locus_tag="O0305_001282"
     CDS             1487..3181
                     /gene="ezrA"
                     /locus_tag="O0305_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCY0274431.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(3255..3395)
                     /locus_tag="O0305_001283"
     CDS             complement(3255..3395)
                     /locus_tag="O0305_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274432.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            3544..4692
                     /locus_tag="O0305_001284"
     CDS             3544..4692
                     /locus_tag="O0305_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCY0274433.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            4692..5915
                     /gene="thiI"
                     /locus_tag="O0305_001285"
     CDS             4692..5915
                     /gene="thiI"
                     /locus_tag="O0305_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCY0274434.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            5959..6729
                     /locus_tag="O0305_001286"
     CDS             5959..6729
                     /locus_tag="O0305_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCY0274435.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLYVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLVFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            6827..7321
                     /gene="tpx"
                     /locus_tag="O0305_001287"
     CDS             6827..7321
                     /gene="tpx"
                     /locus_tag="O0305_001287"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCY0274436.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            7444..8391
                     /locus_tag="O0305_001288"
     CDS             7444..8391
                     /locus_tag="O0305_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0274437.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKIPSQFQGFMTELNQWMDTNRPKK"
     gene            8479..9681
                     /locus_tag="O0305_001289"
     CDS             8479..9681
                     /locus_tag="O0305_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCY0274438.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLVERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(9927..10427)
                     /locus_tag="O0305_001290"
     CDS             complement(9927..10427)
                     /locus_tag="O0305_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0274439.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            10568..11686
                     /gene="ald"
                     /locus_tag="O0305_001291"
     CDS             10568..11686
                     /gene="ald"
                     /locus_tag="O0305_001291"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0274440.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            11810..11908
                     /locus_tag="O0305_001292"
     CDS             11810..11908
                     /locus_tag="O0305_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274441.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(12002..13057)
                     /locus_tag="O0305_001293"
     CDS             complement(12002..13057)
                     /locus_tag="O0305_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0274442.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTECEVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
BASE COUNT         4568 a         1889 c         2417 g         4268 t
ORIGIN      
        1 aaaaaacacc cacaattgtg ggtgtttgta ttgaacgtat tatcttgagt agtactcaac
       61 gattaattgt tcgttaattt cagcaggtaa ttcgctacgt tctggtaaac gtacgaaagt
      121 accagttaag ctgtcagcat caaagtttaa gtactcaggt acgaaattgt tgatttcaac
      181 tgattcaacg atgatgttta atttttgaga tttttcacga actgaaattg tttgaccagg
      241 tttaacagaa taagatggaa tatcaacacg tttaccatct actaagatat gaccgtggtt
      301 aactaattga cgtgcttgac gacgagtacg agctaaacct aatgaataaa caacagcgtc
      361 taaacgactt gctaataaga tcatgaagtt ttcaccgtgt acaccgaatt ttttaccagc
      421 gatgtcaaat gtgttacgga attgtctttc agtcattcca tataagtaac gtaatttttg
      481 tttttcacgt aattgtaaac catattctga taatttttta cgttggtttg gaccatgttg
      541 tcctggtgcg taaggacgtt tttctaattc tttaccagta ccgcttaaag agatacctaa
      601 acgacgagat tttttccagt ttgaacctct gaatcgagcc ataataagac tcctccttta
      661 attctttttt gttgttatga ataaacaaaa aagagtgttg catgcttact aagatatatt
      721 gttttatgtg tcttcacctt atagcgacag ttacgtgaca cgtccgcatt gggaacaaca
      781 tagcgctcaa taatatacaa ctgctacttt cgttaattca cacaaagatt attgtaacat
      841 tttcctgcta actgtcaaca ctttattgaa aatgctgatg tccttttatg cgagttgctt
      901 ttcaataatt ttaacaattg cttcaagatg ttctttgtca ttgtcatcaa atcgatccgt
      961 tataggggca tcgatatcta agacgccgat aattttatca tctttgaaaa ttggaacgac
     1021 aatctcagac ttactattag catcacaagc gatatgtcct tcgaattgat gaacatcagc
     1081 tacaatttgt gtacgacgtt ctgaaacggc tgtaccacat acaccttttc caattggaat
     1141 gtggacacaa gcggggtgtc cttggaaagg tccaagtata agttcatttt gttccaataa
     1201 gtaaaagcca acccaattaa tttgatctag attatcattt aataaggcag acatgttact
     1261 taaaatagca atcatgtgat gttcatcttc aataaggctt gctgcttgtt tctttaataa
     1321 tgtgtagttt gttgggttaa ttgttgtcat atttaaaacc tcttattttc atagatttta
     1381 taatagtata gaatttcgaa aatattatgg caataaattc attttataat ttatccttaa
     1441 gcaaaattac gttataatag taatgataaa ttaggaggag aagcatatgg tgttatatat
     1501 cattttggca ataattgtga ttatattgat tgctgtaggt gtattattct atttacgttc
     1561 aaataaacga caaattattg aaaaagcaat cgaacgtaaa aatgaaattg agacgttacc
     1621 ttttgatcaa aaccttgcac aattatctaa gttgaattta aaaggtgaaa caaaaacgaa
     1681 atacgatgca atgaaaaagg acaacgtaga aagtacaaat aagtatctag ctcctgtgga
     1741 agaaaaaatc cataatgctg aggctttatt agataaattt agtttcaacg catctcaaag
     1801 tgaaattgat gatgcaaatg agttgatgga tagttacgaa caaagctatc agcaacaatt
     1861 agaagatgta aatgaaatta ttgcgttata caaagataat gatgaattat atgacaaatg
     1921 taaggttgat tatcgtgaaa tgaaacgtga tgttttagca aatcgtcatc aatttggtga
     1981 ggcagcaagt ctacttgaaa ctgaaattga aaaatttgag ccaaggttag agcaatatga
     2041 agtactaaaa gctgatggta attatgtaca agcgcacaac catatagctg ccttgaatga
     2101 acaaatgaaa cagctaagat cttatatgga agaaatacca gaattaatta gagaaactca
     2161 aaaagaatta cctggtcaat tccaagattt aaaatatggt tgccgtgatc ttaaagttga
     2221 agggtatgat ctggatcacg tgaaagtaga cagtacatta caaagcttaa aaacagagct
     2281 tagtttcgtt gaaccattaa ttagccgctt agaattagaa gaagctaatg ataaactagc
     2341 taatatcaat gataagttag atgacatgta tgatttaatt gaacatgaag ttaaagctaa
     2401 aaatgatgtc gaagaaacaa aagatatcat tacggataac ttattcaaag ctaaagacat
     2461 gaattataca ttgcaaacag aaattgaata tgtacgtgaa aactactata taaatgaatc
     2521 tgatgctcag agtgttcgtc aatttgaaaa tgaaattcaa agtttaattt ctgtatatga
     2581 tgatatttta aaagaaatgt ctaaatctgc tgtgcgatat agcgaggttc aggataattt
     2641 acaatattta gaagatcatg tcacagttat taatgacaaa caagaaaagc tacaaaatca
     2701 tctgattcaa ttgcgtgaag atgaagcaga agcagaagac aatctgctac gagtacaatc
     2761 gaagaaagaa gaagtgtatc gtcgattact tgcttctaac ttaacaagcg ttcctgaaag
     2821 gtttatcatc atgaaaaatg aaattgatca tgaagttcgt gatgttaacg aacaatttag
     2881 tgaacgtcca atacacgtta aacagttaaa agataaagtg tctaaaattg tgattcaaat
     2941 gaatacattt gaagatgaag caaatgatgt tcttgttaat gctgtttatg cagagaaatt
     3001 aattcaatat ggaaatagat atcgtaagga ctatagcaat gttgataaga gcttaaatga
     3061 agctgaacga ttatttaaaa ataatcgcta taagcgtgcg attgaaattg cagagcaagc
     3121 tcttgaaagt gttgagccag gtgttactaa acatattgaa gaagaagtta ttaagcaata
     3181 gaaactagta tgtagttata cttaaataat atgagcactc tgtcaaattg gactgatgag
     3241 tttaataatt gaagttagcc aacgatacgt tgtctagctt cttttttata tggataaatg
     3301 aaagggacaa taaatataaa tagcaattgt ttaaagataa acgtaatcaa atgtgttgtt
     3361 ttaattaata taagtagtga aaaaagcata atcacacagc tgtttaaata gagtgaaata
     3421 gtctaattct tatttaataa gtagaaataa gattattttg cgctataacc tttatttttt
     3481 gtcggataat agtataatgc ttgagtaaaa caattttaac gattgatgat taatgaaaga
     3541 gaagtgttta ttttgatata tctagataat gcggcaacga cgaaagcatt tgaagaagtg
     3601 ttagatactt atttaaaagt aaatcaatca atgtattata atccgaatag tccgcataaa
     3661 gctggtttgc aggcaaatca attactacaa caagcaaaaa cccaaattaa tgcaatgatt
     3721 aattcaaaaa caaattatga tgttgtattc actagtggtg caactgaatc caataatctt
     3781 gctttaaaag gtattgccta tcgtaaattt gatacagcga aggaaataat tacatccgtg
     3841 ttagagcatc cgtccgtatt agaggttgta agatatttgg aagcacacga aggatttaaa
     3901 gttaaatatg ttgatgtaaa gaaagatggc agtattaact tagaacactt caaagaatta
     3961 atgtcagaca aagtcggttt agtaacatgt atgtatgtaa ataatgtaac tggacaaata
     4021 cagcctattc cacaaatggc taaagttata aaaaattatc ctaaggcaca ttttcatgta
     4081 gatgcggttc aagcattcgg caaaatttca atggatctca ataacataga tagtattagt
     4141 ttaagtggac acaagtttaa tggtttaaaa ggacaaggcg tcttacttgt aaatcacatt
     4201 caaaatgttg aaccaactgt ccatggtggt ggtcaagaat atggcgttag aagtggaaca
     4261 gttaatttgc caaatgatat tgcaatggtt aaagcgatga agatagctaa tgaaaacttt
     4321 gaagcattga atgcatttgt tactgagtta aataatgacg tccgtcaatt tttaaataaa
     4381 tatcatggag tttatattaa ttcttcaact tcaggttcac cattcgtttt aaatattagt
     4441 tttcctggcg taaaaggtga agtattagtt aatgcttttt caaaatatga cattatgata
     4501 tctacgacaa gtgcttgttc atctaaacgt aataaattaa atgaagtatt ggctgcaatg
     4561 ggattatcag acaaatctat tgaaggtagt ataagattat catttggggc tactacaact
     4621 aaagaagata tagcgaggtt taaagaaata tttatcatca tttatgagga aattaaggag
     4681 ttgctaaaat aatgaagtat gatcacttgc ttgttagata cggggagtta acattaaagg
     4741 gttcaaatag aaagaaattt gtaaatcaat taagaaataa tgtaaataag tcattaaaag
     4801 gacttgatgg gtttgtcgtt aaaggcaaac gagatcgtat gtatattgaa cttgaagacc
     4861 atgcagatat aaatgaaata acatatcgat tatcaaaaat tttcggtatt aaatctatta
     4921 gtccagtatt aaaagtagaa aaaacaatag aggcaataag tgcagcggca attaaatttg
     4981 cgcagcaatt tgaagaaaac agcacattta aaattgatgt gaagcgtgcc gataaaaatt
     5041 tcccaatgga tacgtatgaa ttacagcgtg aattgggtgg tgcagtattg aagcacttcg
     5101 acaatatttc agtgaatgtc aaacgtccag atcatgaaat tcgagtggaa gttagattag
     5161 atgcaattta tatgtatgaa gaagttgttc cgggttcagg tggattacca gttggtactg
     5221 gtggtaagac gttactaatg ctttcaggcg gtatagactc accagttgct gggatggaag
     5281 tgatgagacg tggcgtaaca attgaagcga ttcatttcca tagtccacca tttacaagtg
     5341 atcaagcaaa agaaaaagtt attgaattga cacgtatttt agctgaacgt gttggaccaa
     5401 ttaaattgca tattgtacca tttacagaat tgcaaaaaca ggtaaataaa gttgtacatc
     5461 caagatatac aatgacttca acgagacgta tgatgatgcg tgttgctgat aaattagtac
     5521 atcaaatagg ggctttagct attgtaaatg gtgaaaacct agggcaggta gccagtcaaa
     5581 cacttcatag catgtatgca attaataatg taacttctac tcctgtatta cgtcctttat
     5641 taacttacga taaagaagaa attattatta aatcgaaaga aattggtaca tttgaaacat
     5701 ctattcaacc atttgaagat tgttgtacaa ttttcacccc taaaaatcca gtaaccgaac
     5761 caaactttga taaggtagtc caatatgaaa gtgtctttga ttttgaagag atgattaatc
     5821 gtgctgttga aaatattgaa acacttgaaa taactagtga ttataaaact attaaagaac
     5881 agcaaacaaa ccaattaata aacgactttt tataaataaa atcctagagt aaatttaaac
     5941 ataaggggat gttaaactat ggatttgaac ttaacgatga ttataatcat aattttattt
     6001 ggttttatcg cggcgtttat agattcggtt gtagggggtg gcggtttaat ttctacgcca
     6061 gcattattag caatcggtct accaccatct gtggctttag gtacaaataa attggcaagt
     6121 tcgtttggtt ctttaactag tacgataaag tttataaggt ccggtaaagt ggacttatat
     6181 gttgttgcca aattatttgg ttttgtattt ttggcatctg catgtggcgc atatattgca
     6241 acgatggttc cgtcacaaat attgaaacct ttaatcatca ttgcactttc gtcggtgttt
     6301 atattcacat tacttaaaaa agattggggc aatacacgca cgtttactca atttacattt
     6361 aagaaagcca taatatttgc agcacttttt atattaatcg gcttttatga tggatttgta
     6421 ggtgggggaa caggttcatt tatgcttttt gtattgttag tctttggttt tgatttttta
     6481 agtgcagcag gaaatgctaa agttttgaac tttgcttcta atataggtgc gcttgtatta
     6541 tttatggtat taggacaagt agattatgta ataggtttaa ttatggctat aagtatgatt
     6601 gctgggtcat atgctggggc tcattttgct attaaacaag gtgttggtta tgtaaaagta
     6661 ttatttatta ttattactgc tattttaatt ttaaaaaatg cttttgatta tattcaacaa
     6721 tttgtataaa attaaatttg cttttgatta aagcatgaaa attgtaatca aaccataaat
     6781 tgtcgtatga tgtagttaga attttaaaat gcaggaggtc aagtatatga ctgaaataac
     6841 attcaaaggt ggaccaatcc acttaaaagg tcaacaaatt aatgaaggtg attttgcacc
     6901 tgattttaca gtgttagata atgacttaaa tcaagtaaca ttagcagatt atgctggtaa
     6961 aaagaaatta attagtgtgg taccatcaat tgatacaggt gtttgtgatc agcagactcg
     7021 caaattcaac tctgatgctt ctaaagaaga ggggattgtg cttacaattt cagcagactt
     7081 accattcgca caaaaaagat ggtgcgcttc agcaggttta gacaatgtca ttacattaag
     7141 tgaccaccgt gacttatcat ttggtgaaaa ctatggcgtt gttatggaag aacttcgctt
     7201 attagctcgt gcagtatttg tattagatgc agataataaa gttgtttata aagaaatcgt
     7261 tagtgaaggt actgatttcc cagattttga tgctgcttta gctgcataca aaaatattta
     7321 atcattaaag agataaatct taaaatgtat acatcgtgtc catcgttgtc aacagcatta
     7381 aaatagaatt gttttctatg attgctaaga cctatgggca ctttttattg gagagggacg
     7441 aatatggcag aacaacaaac aattatggaa cgcttgtttc atacattaga tgaaaaagct
     7501 aaaacattaa ataatgaaaa tggccaaagt tttattgaaa atcttgggct agcaatggaa
     7561 caagtatata ccaatgaaag aggattgtta gaacaatcga cgttacaaga tcgacgtaaa
     7621 gcattccaat ttgcatattt aagtttaatg caggaagaaa agatacaagc aaatcatcaa
     7681 attacaccag attcaattgg attgatacta ggatttttag ttgagcgttt tatgaacaac
     7741 caagaagaat tacatattgt tgatattgca agtggtgccg gtcatttaag tgctactgta
     7801 aaagaagtgt tacctgaaat tgcggttatg catcatttaa ttgaagttga tccagtttta
     7861 tcacgtgtta gtgtacattt agcaaacttc ttagaaattc ctttcgatgt gtatcctcaa
     7921 gatgccatca tgccactacc attagaagaa gcagatatcg ttattggtga ttttccagta
     7981 ggctattatc caattgatga aagaagtaag gagtttaagc taggttttga agaaggacat
     8041 agttattcac attatttatt aatagaacaa gcaataaatg cattaaaaga tgctggatat
     8101 gcctttctag tggtaccaag taatattttt acaggtgaac atgtaaaaca gcttgaaaaa
     8161 tatattgcaa cagagacaga gatgcaagca tttttaaatt taccaccaac tttatttaaa
     8221 aatgaaaaag cgcgaaaatc tatattaatt ttacaaaaga aaaaatcggg tgaaacaaag
     8281 ccagttgaag tattattggc aaatattcct gatttcaaaa ttccttcaca atttcaagga
     8341 tttatgacag agttaaatca gtggatggac acaaatcgtc ctaaaaaata atctgtaata
     8401 gtatagtcat aaaactgtat gaatgataaa atgaaaaatg aaatagtata aaaagcaaac
     8461 gatggaggat cattacttat gtcaaaatta atcttggcta tcaatgctgg tagttcatca
     8521 ttaaaatttc aattaattag aatgcctgaa gaggaattag taacaaaagg tttagtagaa
     8581 agaatcggat tgaaggattc aatttttaca attgaagtca acggggaaaa agttaaaaca
     8641 gtacaagata tcaaggatca cgttgaggca gttgatatca tgttggatgc ttttaaagcg
     8701 cacaatatta ttaatgatat taacgatatc gatggaacag gtcaccgtgt tgtacatggt
     8761 ggtgaaaaat tccctgaatc agtagctatt actgatgagg ttgaaaagga aattgaagaa
     8821 ttaagcgaat tagcaccgct tcacaaccca gctaacttga tgggtattcg tgctttccgt
     8881 aaattattac caaatatccc tcatgtggca atttttgata cagcattcca tcaaacaatg
     8941 cctgaaaaag catatctata tagcttgcca tatcattatt ataaagatta tggcattcgt
     9001 aagtatggtt tccatggtac aagccataaa tttgtatcac aaagagcggc agaaatgtta
     9061 gataagccaa ttgaagattt acgtattatt tcatgtcaca ttggtaatgg agcatcaatt
     9121 gcagctatcg atggcggtaa atctattgat acatcaatgg gcttcacacc acttgctggt
     9181 gtaacaatgg gtactcgttc tggtaatatt gaccctgctt taattccatt tattatggaa
     9241 aaaacaggta aaactgctga acaagtattg gaaatcttaa acaaagaatc aggtttatta
     9301 ggattatcag gtacatctag tgatttacgt gacttatcag aagaagctga atcaggtaaa
     9361 gcacgttcac aaatggcttt agatgtattt gcttctaaaa ttcacaaata tattggatca
     9421 tatgctgcga gaatgcatgg tgttgatgtt atcgtattta cagcaggtat cggtgaaaac
     9481 tcagtagaaa ttcgtgcgaa agttcttgaa ggcttagaat ttatgggtgt ttactgggat
     9541 cctaagaaaa atgaaaactt actacgtggt aaagaaggat ttattaacta tcctcattca
     9601 ccagttaaag ttgtcgttat tccaacagat gaagaaagta tgattgcccg tgatgttatg
     9661 acattcggtg gtttaaaata atttttaaat aaatattaag cgtctatctc cgctacaatt
     9721 atatgtagga ggtagacgct ttttgtattg tttgaaaatt aactagtgta atgtctcttt
     9781 tcacgaatat cttaaagaat taagtagtcg aagaagatta tgaaaacatt attaagtgag
     9841 ttttatttat aaacaataaa aaaccaagtg cagaaagttg cacttggttc ataatgatca
     9901 agtagttatc taatgatcat ctatatttaa ttttgatatt tttcacgtaa ttgagttgtt
     9961 gcaacttgtg gttggaagtc tgctggtaac tcttcagtac gaacaactaa cacgtcacat
    10021 ggcgcatgac gaacgataga ttctgataca gaaccaacaa taaatctttc cacggcattt
    10081 aagcctgatg taccactcat aatcaagtct gcattaattt catgtgcaag ctttttagga
    10141 atgatagatt taggagagcc aaactctaga cgcgtttcta catctttaac accagcgtta
    10201 gtagctactt ctttataacc atttaataat tcttctgcaa aatgcttaga tttttcagta
    10261 aattgagcat cataaacttc ataagaagaa tacgttcttg aatcaattac attcacaatt
    10321 gttaacttcg catcgttacg tttagcaaca ccaactgctc tgttaaatgc ccattccgct
    10381 tcatgtgaac cgtcaactgc gattaaaata tttttgtaag taatcatagt aagtgcctcc
    10441 ttgttctttc ttaattatat tttaccacaa ttttcggaaa agtcagtgta ataaccttta
    10501 caattttgtg gatttttgat tgcgctttca aaatgttcgc gttaagatga ttatggaggt
    10561 ggatggtatg aaaattggta taccaaggga gattaaaaat aatgaaaatc gtgttggttt
    10621 atcaccaagt ggtgtgcacg ctttagtaga aagtgggcat actgttttag tggaaacaaa
    10681 tgcgggttca ggatcattct ttgaagatgt agattacaaa gaagcaggtg ctgagattgt
    10741 tgctgaacaa gcaaaagttt gggatgtgga tatggttatt aaagttaaag aaccacttga
    10801 atctgaatat ccatatttta aagaagggct tgtattattc acttatcttc atttagcaaa
    10861 tgaagaaaaa ttaacacaag ctttgataga tagaaaagta attagtattg catatgagac
    10921 tgtgcagtta ccagaccgat ctttaccatt gttatcacca atgagtgagg tagcaggaag
    10981 aatgtcagct caagttggcg cagagttcct acaaaaactt aatggtggta tgggaattct
    11041 actaggtggt gtcccaggag tacctaaggg taaagtaact attatcggtg gtggtcaagc
    11101 aggaacaaat gcagctaaaa ttgcactagg actaggtgca gatgttacga ttttagatgt
    11161 taatccaaag cgtttacaac aattagatga tttattcggt ggacgtgtac atacaattat
    11221 gtcaaatccg ttgaatattg agttgtatgt taaacaaagt gatttagtaa taggtgcagt
    11281 tttaattcca ggtgctaaag cgccaagact tgtaacagaa gacatgatta aacaaatgaa
    11341 aaatgggtca gttattattg acattgctat tgatcaaggc ggtatttttg aaacaactga
    11401 taaaattacg acacatgatg atcctacata tattaagcat ggtgtggttc attatgcagt
    11461 tgcaaatatg ccaggtgcag taccgcgtac ttcgacgtta gctttaaata atgctacgct
    11521 accttatgcg ctcatgctag ctaataaagg gtatagagaa gcatttaaat caaatcaacc
    11581 attatcatta ggtttaaata cttacaaagg tcacgtaacc aataaaggcg ttgcagaggc
    11641 atttgaaatg gaatataaat ctgtagaaga agcattacaa ttataatttg aatttgatga
    11701 aaagggaatg gattacagcg tactaaagaa tgacgttatc atctccttgg ttattaaaat
    11761 taaataatga taaattaaag ctagaaacaa tgcgaagaaa tcatcgaaca tgaatgatat
    11821 tgatttgatt cattacatgt tactgatgaa catacctgca tatgtgtttc tagcttgttt
    11881 gttacatatt gcatttagac ttggataaaa atttatgtct taggcgctac aatgttatat
    11941 tggcagtgtt tgactgaatg agaatgcgct tgtaacaagc ttttcatttt tatcccactc
    12001 cttatttttc gtaatgtgtt aatacttcat atccttcatt agtgacaagt atgtcatctt
    12061 caattcttac acctgcaaca ccaggtacat aaatacctgg ttcgattgta ataaccatgc
    12121 cagcttctaa caaattagaa ttagtacttg aaacatcttg atattcatgt tcttgtaatc
    12181 ctaggccatg acctaagcga tgagggaaat attcaccata acctttttct gaaataatat
    12241 ttctagcgat atgatcgata tcttttaatg gtattccagg tttaattgct tggattgcag
    12301 atgtttctgc ttctaataca atattataaa tttcttgtgc ttctttgcta ggttcaccaa
    12361 atttaatagt acgtgtcata tcgctacaat aatgctcata aattacacct aaatcaaata
    12421 gtacatattc attgcttttt aatctgcgat ctcctggtgt gccatgaggt gatgcggcat
    12481 gatctccaaa taaaaccatc gtatcaaaac tcatttcatt gacgccatat tgtttgatag
    12541 tttgctcaat atggttgact acttcacatt cagtcacacc ttcttttaaa taagaaacac
    12601 ctatttcgat acacttatct gctaactcag cagctttacg tattttgcta atttcatctt
    12661 cggatttaat atttctcaat tgtttgattg ttaaatcaac atctccgaat gaattgacat
    12721 tgaaaccaga gattaattgt ttttggcgtg ctactgttaa gtgctcgctt tcaattagta
    12781 atttattgat tgtttgagga taaagtgaaa aagggttttc agtgtctaaa tatccaacga
    12841 tttcacctgt gaaaggtgat gctttgactt cttcgacttc catttttgga caaaatagta
    12901 cttgtttacc atctttctta atcaataatg caaataatct ttcatggggt tcgctacggt
    12961 atccagtaaa ataatataca ttcaacggtg ttgtaatcca tgctgcatca gcttgttgat
    13021 tgttcaattc gtctattatt tttgatattt ttgtcatttt ataaacactc ctcaacataa
    13081 tattacttcc attttatgcc aattattatt agatttcaaa atcgagctaa tacattaact
    13141 ag
//
LOCUS       DAKTIP010000060        12909 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAKTIP010000060 DAKTIP010000000
VERSION     DAKTIP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12909
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.060"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..160
                     /locus_tag="O0305_001294"
     CDS             <1..160
                     /locus_tag="O0305_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0274443.1"
                     /translation="PTNKAPKLLLKGTGNLKGSSVGYKKIEFTFLENKNENIYFTDSL
                     HLEPSEDK"
     gene            224..994
                     /locus_tag="O0305_001295"
     CDS             224..994
                     /locus_tag="O0305_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0274444.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAKIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTKTT
                     KGYYYVNAIKNDKDGRPQENEKRYPVKMVDNKIIPTKEIKDKNIKKEIENFKFFVQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKAPKLLLKGTG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDGLIFKPSEDK"
     gene            1061..1828
                     /locus_tag="O0305_001296"
     CDS             1061..1828
                     /locus_tag="O0305_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0274445.1"
                     /translation="MKRLNKLVLYISFLILVISFTAGCGIGKEAEVKKSFEKTLSMYP
                     IKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMVVQPKGERMKSKGMVLYMNRNTKTTT
                     GKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYGS
                     FKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSGD
                     LKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            1922..4159
                     /locus_tag="O0305_001297"
     CDS             1922..4159
                     /locus_tag="O0305_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274446.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFHHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFQPTNHQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFQVNEVLAYDVIPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKIFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHRLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCKNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEEGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4310..5488
                     /locus_tag="O0305_001298"
     CDS             4310..5488
                     /locus_tag="O0305_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0274447.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHV
                     LGVHVMSTMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEIKR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5490..6878
                     /locus_tag="O0305_001299"
     CDS             5490..6878
                     /locus_tag="O0305_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274448.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRVIQGVSAACIMPSTLAIMKTYYQGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEITNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLNVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7371..9032)
                     /locus_tag="O0305_001300"
     CDS             complement(7371..9032)
                     /locus_tag="O0305_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCY0274449.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9358..11133)
                     /locus_tag="O0305_001301"
     CDS             complement(9358..11133)
                     /locus_tag="O0305_001301"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCY0274450.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHISGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKIDVTTSQKIAREILIDRNGNAE
                     SIKLTINDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITINDSAWQMSFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSPFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            complement(11307..12179)
                     /locus_tag="O0305_001302"
     CDS             complement(11307..12179)
                     /locus_tag="O0305_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0274451.1"
                     /translation="MNKLQDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCGFVIYHTALNFGEALISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVFIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            12292..>12909
                     /locus_tag="O0305_001303"
     CDS             12292..>12909
                     /locus_tag="O0305_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0274452.1"
                     /translation="MAKYKDIASDIRDKIITGDWFYGMKIPSHRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSQQRSQYTVQLINKIE
                     TDDSYIHISKGELGISLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHS"
BASE COUNT         4467 a         1984 c         2340 g         4118 t
ORIGIN      
        1 accaacaaat aaagcgccga agctgttgtt gaaaggtaca gggaatttaa aaggttcatc
       61 agttggatat aaaaaaattg aatttacttt tttagagaat aaaaatgaaa atatttactt
      121 tactgatagt ctacatcttg aaccgagcga ggataaataa tcatctttta accagaatat
      181 gaaatcaatc taggacaacc taataaaagg agagagttgt aagatgatga aacgattaaa
      241 caaattagtg ttaggcatta tttttctgtt tttagtcatt agtatcactg ctggttgtgg
      301 cataggtaaa gaagcgaaaa taaagaaaag ttttgagaag acattgagta tgtatccgat
      361 taaaaatcta gaggatttat acgataaaga aggttatcgt gatgacgaat ttgataaaaa
      421 tgataaaggt acatggatta ttggttctga aatggcaact caaaataagg gggaagctct
      481 gaaagttaaa ggtatggtct tgtatatgaa tagaaatacc aaaacaacaa aaggatatta
      541 ttatgtaaat gcaataaaga atgataaaga cggaagaccc caagagaatg aaaaaaggta
      601 cccagttaaa atggtcgata ataaaatcat tccaacaaaa gaaattaaag ataaaaacat
      661 aaaaaaagaa atcgaaaact ttaagttctt tgttcaatat ggaaacttta aagatttgtc
      721 gaagtataaa gacggagata tttcatataa tccagaggtg ccgagttatt cggctaaata
      781 tcaattaact aatgatgatt acaatgtaaa gcaattacgt aaaagatatg acataccaac
      841 aaataaagca ccgaagttgt tgttgaaggg tacagggaat ttaaaaggtt catcagttgg
      901 atataaagac attgaattta cgtttgtaga gaaaaaagaa gagaacattt actttagtga
      961 tgggttaatt tttaaaccaa gtgaggataa ataatcatga ctcgatcaaa atatgaaatc
     1021 aatccaggaa agataataag aagaatagga ttgtgaaatt atgaaacgat taaacaaatt
     1081 ggtattgtat attagttttt tgatattagt cattagtttc actgctggtt gtggcatagg
     1141 taaagaagcg gaagttaaga aaagctttga aaaaacattg agtatgtatc cgattaaaaa
     1201 tctagaggat ttatacgata aagaaggtta tcgtgatgac gaatttgata aaaatgataa
     1261 aggtacatgg attattggtt ctgaaatggt tgttcaacct aaaggggaac gtatgaaatc
     1321 aaaaggtatg gttctatata tgaatagaaa cactaagact acaaccggga agtatatagt
     1381 gagtgaaaca cttcatgatg aagatggtag acctaagagt aaagataaag aatatcccgt
     1441 taaaatggta gacaataaaa tcattccaac taaaggtatc aaagacgaaa atataaaaaa
     1501 agaaatcgag aattttaagt tcttcgcgca atatggcagc tttaaagatt tgtcgaagta
     1561 caaagacggc gatatatcat ataatccaga ggtaccgagt tattcagcaa aatatcaatt
     1621 aactaatgat gactataatg taaaacaatt acgaaaaaga tataaaatac caacgaataa
     1681 agcaccaaag ttattgttga aaggttcggg agacttaaaa ggttcatcag ttggatataa
     1741 agacattgaa tttacttttg tggagaaaaa aggtgaaaat acttttttta ctgatagtct
     1801 acatcttgaa ccgagtgagg ataaataatc atccacacac acgattcaat atgaaattca
     1861 aatatgttgc tgtaaaataa tgtaaaataa acgtattcat attactgaat tgagggatag
     1921 tatgcaacgt gattatttaa ttcgagtaga aactgagagt atgtcagatt tcaaaaggct
     1981 caatagttta atgattggtt ttgttattaa aggtgaggca cacatttatg atgaaaataa
     2041 tatgacgcaa tgcaacagtg gcgacatttt catcattaac caccgcgact tgtatcgatt
     2101 tcaacttcaa caagatggca tcatatgtta tatccaattc caaatgaaat atttagcaga
     2161 caagtttgat gatgtgcatt gtctatattt tcacttaaca gatgcgacca caacaaagaa
     2221 tatacatcaa ctgagaaata taatggcaag actggtttca acacatatcc gacataatga
     2281 gttgtctaaa ttgactgagc aacaacttgt gattcagttg cttatgcata tgattcatta
     2341 tgtcccgcgt acatatcatt cgaaccaaag tatcttaaat gatgataaag tgaatcaagt
     2401 atgcgactat atcgagttac attttcatga agatttaagc ctttcagaat taagcgaata
     2461 cgttgggtgg tcagagagcc atctgtctaa aaagtttaca gaatcgctag gtgtaggatt
     2521 ccatcatttc ttaaatacga cgcgaattga gcatgcgaaa ctcgatttga catacacaga
     2581 tgaaaccatt actgatattg cattgcaaaa tggcttttca agtgcagcga gctttgcgag
     2641 aacatttaaa cactttacgc atcaaacacc taaacaatat cgaggtgatc gtccagcaat
     2701 cactgaaaac cagcaatcgg cacaacatga ttatcacgac cgtgaattga tattactttt
     2761 aaatgactac attgaagaaa tgaatcattt tattgaagat attgaaaaga tgaactataa
     2821 agagattgcc tttcaaccaa ccaatcacca actaaatcaa tttaatcata ttattcaagt
     2881 gggctatttg aggaatttgc tcaatacaca gtatcaatca cagttgctta catgtcatca
     2941 tgattttcaa gtcaatgaag tattagcata tgatgtgatt ccatatatca tgaaaaagct
     3001 caatgcgcca ttcacgtatg atgcagaaat ttcgaatata ttttatgata ttgatttgtg
     3061 tttagacttt ttattagatc ataactttag tttgaccatg catttggatc agtatgactc
     3121 acgtgattat atcgatgcat ttaaaatttt tatccatcac gttgccctgc atgtcagtca
     3181 tagaaaagat ttgaagttca acttgtatgt gacgacattg cacacgtctt tgattgaaat
     3241 gattgattat tttaaagcat tattccctaa tggtggcttg tacattcact tagatcaagc
     3301 tacggaaaga catctaccat tattgaaacg acttgagcca cacatcgacc attttgtatt
     3361 tgatgccaat tcaaatgacg ctgttgattt taataaaatg aatgatgatg aatttaaaac
     3421 cgcgagtcaa atgattatta ataaaacgaa ttaccttatc gacttaatgc atcgtcatcg
     3481 cctaaagcgt ccactcattt tactcaattg gaatacattg acgggtgata catttattac
     3541 aaacggcgaa tattttagag gtggtatcat cattgagcaa ttattaaaat taagttctaa
     3601 agtagagggt atcggttatt ggttgaatta tgatttgcac gtcagtcatt gtaaaaatga
     3661 acgggattat atgaattcta ttgaactatt tcatcaatat aatggaaaac gtccggtcta
     3721 tttcacggca ttgctattta ataaattaac aagcaatatt ttgtattctg atgatacatg
     3781 tattgtcacg gggactgatt caaattttca aatattgtta tatgatgcaa agcattttaa
     3841 tccgtactta gcgttggaca atcaaatgaa tatgcgtgcg acggaaatga ttcatttgaa
     3901 cattaatgcc ctggaagaag gtatgtataa gattaaacat tttaccttag ataaagaaaa
     3961 tggtgcgtta tttaatcttt ggcgcaaaca tcatacgata catggcatgg acaaggactc
     4021 tatagattac gttaatcgaa tgagttttcc gaaattagaa gtatatgata tagatatcac
     4081 ggacacactg gcattaaaca ttaaaatgat tacgaatggg attcacttaa ttgaagtaaa
     4141 acgttaccca agttcataaa atgatcacaa atcacaaatt ttgatataca taatttgtga
     4201 ttttttatat tcaaagctaa aattgcaaaa aattaatggt taacatctct gttgttggca
     4261 atataaataa tgattaatca tttatgatgt aactaaggag atgaaggata tgaatcaaca
     4321 attaattgaa actttaaaat ctaaagaagg caaaatgatt gagatcagac gttatttaca
     4381 tcagcatcca gaattatctt ttcatgaaga tgaaacggcg aaatacatcg ctgaatttta
     4441 caaaggtaaa gatgtggaag tagaaacgaa tgtcggacca cgtggaatta aagtaacgat
     4501 tgattcaggg aaacctggta aaacattagc aatccgtgca gactttgacg cattacccat
     4561 tactgaagat acaggattat cttttgcatc acaaaataaa ggtgttatgc acgcatgtgg
     4621 tcacgatgca catacagcat acatgcttgt attagcagag acgcttgctg aaatgaaaga
     4681 tagttttaca ggaaaagtcg ttgtgataca tcaaccagct gaagaagtac caccaggtgg
     4741 tgctaaaaca atgattgaaa atggtgtatt agacggtgtt gatcatgtat taggtgtaca
     4801 cgtcatgagc acaatgaaaa caggtaaagt gtattacaga cctggttatg ttcaaacagg
     4861 acgcgcattc ttcaaattga aagttcaagg taaaggtggt catggttcat caccacatat
     4921 ggccaatgat gccattgttg caggtagcta cttcgtcaca gcgttacaaa cagttgtatc
     4981 tagacgacta agtccatttg aaaccggtgt tgtcacaatc ggttcatttg acggtaaagg
     5041 tcaattcaat gtcattaaag atgttgttga aattgaaggt gatgtacgtg gattaacaga
     5101 tgctacaaaa gcaacaattg aaaaagaaat taaacgttta tcaaaaggat tagaggatat
     5161 gtatggtgta acttgcacct tagaatataa cgatgattat ccagcattat ataatgatcc
     5221 agagtttact gagtacgtgg ctaagacgtt gaaagaagca aaccttgatt ttggtgttga
     5281 aatgtgtgaa ccacaaccac cttcagaaga ctttgcatat tatgctaaag aacgcccaag
     5341 tgcctttatt tatacaggtg cagcggtgga aaatggtgaa atttacccac atcatcatcc
     5401 taaatttaac atttcagaaa aatcattact tatttctgca gaagcagtag ggacagttgt
     5461 tttagattac cttaaaggag ataactaaca tgaatgaaac gtatcgcggg ggcaacaagt
     5521 taatcttagg tattgtatta ggtgttatta cattttggtt gtttgcacaa tcacttgtaa
     5581 atgttgtacc gaatttacaa caaagttttg gtacagacat ggggacaatt agtattgcgg
     5641 tcagtctaac tgcactattt tcaggcatgt ttgttgttgg agcaggtggt ctggcagata
     5701 aaattgggcg cgtgaaaatg acgaatatcg gtttattatt aagtattatt ggttcagcat
     5761 taattattat tacgaattta ccggcattat taattttagg tcgtgttata caaggtgtat
     5821 cagcagcgtg tattatgcct tctacattgg ccattatgaa aacttattat cagggtgctg
     5881 aacgtcagcg tgccttaagt tattggtcta tcggttcttg gggtggcagt ggtatctgtt
     5941 cactcttcgg tggtgcagtt gcgacaacta tgggttggag atggattttc atcttctcaa
     6001 ttatcgttgc cgtactttca atgttactca tcaaagggac gcctgaaacg aaatcagaaa
     6061 ttaccaatac acataaattt gacgttgcag ggctaattgt tctagtagtt atgttgctaa
     6121 gtttaaacgt tgtcattact aaaggtgcag cacttggtta cacatcatta tggttctttg
     6181 gtttgattgc aatcgtaatt gtagcattct ttattttctt aaatgttgag aaaaaagtag
     6241 ataatccact tattgatttt aaattatttg aaaataaacc atatacaggt gcaacgattt
     6301 cgaacttctt attaaacggt gttgcaggta cattaattgt agcgaataca ttcgtgcaac
     6361 aaggtttagg ttatacagca ttgcaggcag gatacttatc aattacttat ttaatcatgg
     6421 tgttattgat gattcgagtt ggtgaaaaat tattacaaaa aatgggttct aagcgaccaa
     6481 tgttattagg tacattcatt gtggtcattg gtattgcact tatttcatta gtattcttac
     6541 caggcatatt ttatgttatc agttgtgtcg taggatattt atgtttcgga ctaggcttag
     6601 gtatttatgc aacaccttct acagatacag ctatttcgaa tgcaccgtta gataaagttg
     6661 gcgttgcttc aggtatttat aaaatggctt catcacttgg tggcgcattc ggtgtcgcaa
     6721 ttagtggtgc tgtatatgct ggtgcagttg ctgcaacgag cattcataca ggtgcgatga
     6781 ttgcactttg ggttaacgta ttaatgggaa tcatggcatt tatcgcaatt ttattcgcga
     6841 ttcctaatga tgataaacgt gtcaaagatg cgaaataata acgatgacac gccaagtgtt
     6901 ttgataatga tataaatgaa agtaaagcaa attaaatgag gaaatgtata gagcagtgca
     6961 tatagataaa tctatgattg gattaacact aatataaata taaatgaaaa tgaaaaacca
     7021 tccacttaaa tttttgataa gttaggtgga tggttattta ttgcgttgtt ctaaaaattt
     7081 ggacatttat atggaaagaa ctatatataa tgtgaaggtt atggcaattc gcttcaatga
     7141 aggcgacgtg taaaagtgta tattaaacgt ttcggggtca gactccgaga acagtgtccg
     7201 tcatgcttac cgcatactaa tgaagcatta ttatatcagt gtttatagcc gatttaagat
     7261 aataaatttt gctaaacaaa aaagccaacc catgaatgtt ggattggctt tttacatgca
     7321 tctgaatctc taattttaaa aaaatatgaa tataaataag acagtaaaaa ttaaatttca
     7381 gttgttgcaa tttcttcatc tgtaggtaca tcatcgttaa ggccaacaag tgcttcagaa
     7441 acatttcgtg aatgataacc gatacgttca agaacaccaa tcatatcgat atatagtaat
     7501 ccgccttttg ttgtacattc accacgatta aggcgtttaa tatgaccttt gcgtagttta
     7561 tgttcaatat taaatgattc tctactacgt tctacaattt catctttttt cgttttgtca
     7621 taaacatcta acatgtcgat ggctttatca aatgactcag caacatggtt gaataattta
     7681 tccataccgc gttgtgcatc ttctgtaatg cgaatatctt catcatgttg gcgttttaat
     7741 tgagcgacat actcttctgt tagctctgct acttttaaaa tagagcgatt gacatcaaac
     7801 ataactgcta aacgctcaac gtctgccttc gtaatggctt ttgtagaaat tctaactaaa
     7861 taatttcgaa tgctatcatt gattgtttca acagcttgat gcttttgttc aagctttttg
     7921 atcaattttt tatcgtcttt tgtaatttcg cgaatgtctt caaacattga taagacaatc
     7981 tgacccacat tttgtaattc tttttgagtt tcttgtaatg caacaccagg tgcgtgataa
     8041 acaagatctt tgtttaagtg ctgaggttta tagtcatcag caatatcttt acctgggaca
     8101 agctttgtaa caatccatgc taaacctgct acaaatggta attgaatcaa agtatttgtt
     8161 atgttgaaga taccatgtga tactgcaatc gtcatcgctg gttttaagtg ccataaatct
     8221 tgtaacaaac taatcaaatg aatcacaact ggcaagaaaa ttgtgaagat aattaccccg
     8281 attaagttaa agatgacgtg tacaagcgcc gcacgttttg cagcgattga gccggctaaa
     8341 ctagctaaga tagctgtaat cgtggtacca atgttatcgc ctagtaacac agggattgct
     8401 gcgtttaagc taattaaatc ttgttgataa aattcttgta aaataccaat cgtcgcactt
     8461 gaactttgaa ctagtgctgt taaccctgcg ccgacaatga cagcgagtat tggatttgta
     8521 gacatatcaa gcattaattg cttaaatcca tctaatgatg ctaaaggttt aacggcatca
     8581 cccataaatt ctagaccgaa gaatagtgaa ccgaaaccga atagtatgcg gccaatgtta
     8641 ttgattttag agcgtttaaa gaaaaagatt aagaatgcac ctaatgctaa aattggcatt
     8701 gcatattcgc ctaaatctat accgataata aatgcagtta ccgttgttcc gatattagca
     8761 cccattatca ctccaatggc ttgtttcaat gtcataaatc cagctgttac cagtccgatt
     8821 gtgataactg tcgtacctga actactttgt attaaaatag ttacaacgat acctgcaata
     8881 acacctaata ctggatttga tgtaaatttg tttaaaatat ctcgtagcct gtctcctgct
     8941 gatgcttgaa gcccgtctcc catgattttt aagccgtaaa ggaaaatacc taaaccacct
     9001 aaaaaggaga aaatgacttc tgtaaccgac atttccatta tttcacctca aataagcttt
     9061 atatttagat tatcgcttat aattgtaaat ttaatgttaa gattaggtaa aattatttaa
     9121 caatatatgt tatttgtata tgacttgtaa aatatcgtca cttattatgt aaattttcag
     9181 tgtgaaatgg caggtttgca atcacttgtt taacaaaatg atgcaatcaa tcatgtaatt
     9241 atgtttcatc aaaaaaatca tgagagtgga acaacgaaat aatttttatg aaaacatcat
     9301 ttctgtccca gtctcatgat ttgaaatcac caaataaaaa tctattaatg gttttcgtta
     9361 taacaatttg tgttctttta ataatgactc aatgtacgta ccttttatct ttttaaggaa
     9421 tcctgctaat gcgagttttt gcattttcga atctttagta atctcacgca aatcttggtg
     9481 gtcattcagt tcgtatatgg catccattaa gacgcgaaga tcaaatggac tattgatgac
     9541 ttctggaata ccacgatcta tatttagtaa ttgataaaca gcttccatgg cagtacgaac
     9601 cgaatattct gttgtaaata cagtgtctcg ctctgtttct gcaaagttac caataaatgc
     9661 taagttctga gattgatgcg ggacgactaa aggtctgtcg ccgatagcac gcgtcatgaa
     9721 ataagatgtg atatatggca tataaacagg aatcgtatta gatgcatgtt ttgctaagtc
     9781 ttcaattttg tcagttgata cacctaagtg atacagccat tcttggcata tttcattacc
     9841 actacattct gtaattggct ttttaatata atcgccgttt acatctgaat ataaggcata
     9901 aatccatgta gatatttcat tttcaggttg gtctttaaac tgttgctgac gattgattgt
     9961 aaaactcatt tgccatgcag aatcattgat tgtaataata ccgcctgtaa ctgttttgcc
    10021 tgcaagtggg tcacgtttac aaatactttc tattgtatcg ataatctctt tattgtttgt
    10081 tgtagaagtt gctgaaacaa accaactttt tttaggaata ttttggcaaa acttatcagg
    10141 attaccaaat tcaggacttt gtcgcgctaa atttttccat agtgtccaac taccacctaa
    10201 ttcgtcagtt ggtggcgctg gtgtatcatt atcaccataa gtagagcttt ctgtaatact
    10261 accgtttgtc acaaagacaa gatcgtttat agtcagttta atagattctg cattaccatt
    10321 acggtcaatt aatatttctc gggcaatttt ttgacttgtc gtaacatcta ttttaatatc
    10381 ttcgactttt acatcgtatt caaattgaac cccatgcgat tttaaatatt caaccatagg
    10441 taatactaaa gattcatatt gattatattt agtgaatttt aaagctgaaa agtctgcgag
    10501 accactaata tgatgaacga atcgcattag atagcgacgc atttccattg cagaatgcca
    10561 cggttcaaat gcaaacatcg ttttccagta aatccaaaag tttgaattaa agaagtcatc
    10621 ggaaaataca tctgttattt tgacatcatc taaatcttct tcattcgtta agcataaatc
    10681 taaaatttct ttaatcgccg ttttagtcaa agtgaagtct ccgtctgtga ctaaacgttg
    10741 accctgtttc tcaataacac gacagcgaga atagttaggg tcttctttgt ttagccaata
    10801 gaactcatct aatacagacg cgttatcgat ttctaatgaa gggatagatc tgaataagtc
    10861 ccacaaacat tcaaagtggt tctccatttc acgaccaccg cggacaacat agccttttaa
    10921 aggcatattt tcaccatcaa gactaccacc tgctttaggt aactcttcta aaatatgaat
    10981 cttcgaacct tccatttgac catcccttat taaaaaacaa gctgcagcaa gtgaagctag
    11041 accagatccg attaagtaag cggatttgtt ttctacattt tcaggttttt tagggcgcgc
    11101 aaatgcttca taatttccat aactgtaata catatccacc aagtcctttc gtattagaat
    11161 acactcagac tatacccctt tgtgaattgc aaatattagt atttatattg gtaaatttga
    11221 cgaaattaga tcatttttat atttttgatt tttaaaattt tatcactcga atgtttttgg
    11281 attctgaggt gcgggacttg gaataattat gtattttgaa agaagttact taaactaacg
    11341 cctagtagag tgataattcc gcctataata gcaagggttg ttggtagctc gtctaataac
    11401 agataagata ataataaaga aacgataggt gttaaataaa gagacattgt tgcatcagag
    11461 acaccaactg acttcacaat ataagcaagc aaaacgtatg gaattatagt agggaatata
    11521 gctaaataaa gtaccgatac tattgatgta aaagtggcgc cgtgtatatc gttgatgatt
    11581 tcaggaataa aaataagcat aaatggtgag cttgccatta ttgtatatag tgtgaaagcg
    11641 atgaagccgt atttttctat gtattttttc tggaaagtaa aatacaaact ttcactaaaa
    11701 gatgcaagta aaataataaa aacacctaat acattaatag ttgtgtaatc atctttactt
    11761 attgaaataa tggatattcc tataaatgcg acgagtgaac ttaaccaatt ccattttgaa
    11821 aaatgctctt ttaaaaatat ataagctaaa gcactagaaa aaataggcgt tgtagagact
    11881 agaattccag atatacctgc actaatcaaa gcttcaccaa aatttaaagc tgtgtgatat
    11941 atcacaaatc cacaaaatcc taaaataaaa ataacaggga tatctcttag ttcaggggta
    12001 ggcaatttct ttataattac gaacggcaag agaattattg ttgctaaaat taaacgaaat
    12061 gccgacaatg attctgcact aaaatcattt aacgcaatct ttatcattgg aaatgcagat
    12121 ccccacaata tgatagtaaa taaatatgat agaaaagtag tgtcttgaag tttattcatt
    12181 aatatcatca ctcctttaat tatgtgtttc tatattaaaa aatatgattt aaaatgagta
    12241 caaccaattt tgaatggatt tacctatcca attttaaaag ggagggagaa gatggctaaa
    12301 tataaagata ttgctagtga cataagagat aaaataatca caggggattg gttttatgga
    12361 atgaaaatac cttcacatag gcagttggcg atacagtaca acgtaaatag agtaacgatt
    12421 attaaaagta ttgagttatt agaagctgaa ggatttatct atactaaagt aggtagtgga
    12481 acatatgtta atgactattt gaatgaagca catattacaa ataagtggtc tgaaatgatg
    12541 ttatggtcct ctcaacaaag aagtcagtat acggtgcaat taattaataa aattgagaca
    12601 gatgattcgt atatacatat aagtaaaggt gaattgggta tatcgttaat gccacatatt
    12661 caattgaaaa aagccatgtc taatacagcc agtcatattg aagacttatc ttttggttat
    12721 aataatggct atggttatat caagttaaga gatattatcg ttgaacgaat gtcaaagcaa
    12781 ggtataaatg taggtagaga aaatgtaatg atcacttcag gcgctttaca tgccattcaa
    12841 cttttatcta ttgggttttt aggtcaagat gccataataa tttcgaatac accatcatat
    12901 attcactct
//
LOCUS       DAKTIP010000061        12814 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAKTIP010000061 DAKTIP010000000
VERSION     DAKTIP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12814
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.061"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            48..1877
                     /locus_tag="O0305_001304"
     CDS             48..1877
                     /locus_tag="O0305_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCY0274453.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSAKNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     LPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(2017..4095)
                     /locus_tag="O0305_001305"
     CDS             complement(2017..4095)
                     /locus_tag="O0305_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0274454.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(4233..5642)
                     /locus_tag="O0305_001306"
     CDS             complement(4233..5642)
                     /locus_tag="O0305_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0274455.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            5928..6734
                     /locus_tag="O0305_001307"
     CDS             5928..6734
                     /locus_tag="O0305_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCY0274456.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(6797..7453)
                     /locus_tag="O0305_001308"
     CDS             complement(6797..7453)
                     /locus_tag="O0305_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCY0274457.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            7566..8039
                     /locus_tag="O0305_001309"
     CDS             7566..8039
                     /locus_tag="O0305_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0274458.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(8162..9373)
                     /locus_tag="O0305_001310"
     CDS             complement(8162..9373)
                     /locus_tag="O0305_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0274459.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(9555..10214)
                     /locus_tag="O0305_001311"
     CDS             complement(9555..10214)
                     /locus_tag="O0305_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274460.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(10274..11416)
                     /locus_tag="O0305_001312"
     CDS             complement(10274..11416)
                     /locus_tag="O0305_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCY0274461.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            11684..12070
                     /gene="gtxA"
                     /locus_tag="O0305_001313"
     CDS             11684..12070
                     /gene="gtxA"
                     /locus_tag="O0305_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCY0274462.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFLVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLIITIPITFILSKWILKDSN"
     gene            12204..12311
                     /locus_tag="O0305_001314"
     CDS             12204..12311
                     /locus_tag="O0305_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0274463.1"
                     /translation="MFNLLINIMTSAISGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         4281 a         2000 c         2313 g         4220 t
ORIGIN      
        1 ggcgtatagt gatatccatc aaaaactata aaaaggaata ggagattatg ttcaatcaat
       61 ttaaaagact tattataggg caacctaaaa aaaacagaga actaaaagac gaaaagatat
      121 cgaagtttaa agggttagcg attctttcgt cggatgcatt atcttcagtg gcttacggtc
      181 ccgaacaaat actgattaca ctatcagtag tgggtgcagt tgcaacttgg tatactttac
      241 cgattgcagg tgctgttctg atcttattag ctgctttaat tatgtcttat aggcaaatta
      301 tttatgcata tcctaaaggt ggcggcgcgt atatggtgtc caagaccaat ttaggagaaa
      361 aatggggatt gcttgcagga ggctcattat tagtcgatta catactgact gtcgctgtta
      421 gtatatcatc tggtgctgat gcatttgttg ctgcattccc aagtttatat gggcataaag
      481 tactgattgc atgtttactt gtacttttta ttttaatatt aaatttacgt ggtttaactg
      541 aatctgccac cgtattatcc tatccagtat atcttttcat tatagggtta gtgatattaa
      601 tatttatagg tactttccgt gtggcgacag gagatattca accacatatg catgcatcag
      661 taggaactgc ggttcctgga gtaacattat tcttgctatt aaaagcattt tcatcagggg
      721 cttcatcgtt aacgggtgtt gaagctattt caaatgcggt aaccaatttt agagagccaa
      781 gtgctaaaaa tgctgtgaaa acgttaattg ctatgggttc aattttagcg tttttattag
      841 ttggtatcgt tggtttagca tatgtttatg gaattttgcc acaaacggaa acgacagttt
      901 tatcacaatt agctatgcaa atatttggtg ataatgctgc cttctatttt gtacaggcaa
      961 cgaccgtgat gattttggta ttagcagcca acactggatt tacagctttt ccgatgttag
     1021 cggcttcaat gtctaaagat aaatatatgc ctagaatgtt tactgttaga ggtgatcgct
     1081 taggctactc taactcgatt attattttag gtgtattagc cattatttta attattgtat
     1141 ttgatggtat gactgaagat ttaattccat tgtatgcggt tggtgtattt attccattta
     1201 cactagcaca attcggtatg gttattaaat ggattcatga acgtcctaag aattggttaa
     1261 gtaagttgtc tgtgaactta cttggtggta tcgtgacatt tattgttttt atgattttac
     1321 tcataactaa atttagccaa gtatggccga tacttatttt cttgccgttc gtggtgattt
     1381 tcttcttgaa aattaacaaa cattatcgag atatcgcaga acaattacgt tctgacattg
     1441 atgttcttaa tgtggacgtg gtagatcgta atttagcaat tgtgccgata acaagtatta
     1501 caacagcagt tgataagtct atttattacg cacaaatgct tgcgaataat gatgtcatag
     1561 cagtacatgt atcatttgga gatgaagatg aaaaagcatt ccaagagaaa tggaaacgtc
     1621 atttcccaga tgtaagatta gttattttac attcggaata tcgcagtatt atacgaccaa
     1681 tttcacgttt tatcgataag attaatcgta aagccaacga tcaaaactat atgattacag
     1741 tagtgatacc agaatttatt actaagaaac gttggcataa tttattacac aatcaaacta
     1801 gtttgcgtat gaaattgtat ttaatttatc aaaagaatgt taacgtatgt acaattccat
     1861 ttaaacttaa aaaatagatt ctataatcaa atcggattga tgaaaggtta tttacttttc
     1921 atcaatctga ttttttgatg ttgaatataa aaaccccgta catatgagat gataatgact
     1981 ttgtctcata tgtacggggg tattgttatg tcttgcttaa tgtttggact gtaaagacaa
     2041 gacaatttcg ttgtaattga tattttctcg aatttgagct gcaacgatat tgttgatttt
     2101 atttttgcga attaattcat ctaaaagatg acgttgtaag tacaatgctt ccaatttgtg
     2161 gttatggaac atagcttgcg gtatgatttg attgctgttt tcttgacgtt cttttcgacg
     2221 ttgtgcacgc cactttaatg agcgactcat atcatagtaa tgattaatga ctaagctaac
     2281 ctcaagtaca tttgtactat tttgttcttc ttttaagcgt aaaataatat tgtgattcac
     2341 aatacgcata attttttgaa catctttgaa tgaattttca aattcttgct tcttagattc
     2401 tttatttaag gctttgtagt gcttcatcgc tttaagtcta agccataaca tcttgaagaa
     2461 gtttgcaaat ttttcaacaa atgatgcagt acggtgtgtc tctgttaatt cgacaatatt
     2521 gcggtaatta ttaatatcct gatatgttgc cttaccttta tcaattaaac gctcaagtgt
     2581 agatgtttcg attacttttg caatatcttc taaacgtttg agttcttttg tattttgatt
     2641 atcaggttct gaatttaata aaaataacaa ttctccatag tattggttta atactgggcg
     2701 ataatttgta tcgtttttgt cttttttact ttcgttttta aaatgctgga tcactttttg
     2761 aacaatgaaa attttggcag attgataagt cataccttta aaagtagtat cttcggcaga
     2821 tggtgtaatt aaaggtaaaa caatttgcgc taagattaaa ctaattaata ccatgaaaga
     2881 tgcaataaac aataaatcat tacggtattc gaatgcttgt ccttttgtaa tgataaatgg
     2941 taatgtaagt gccattgaaa gtgaaattgt accgtgaata ccacacatgg tcataataaa
     3001 tgcgtaacgc actcgagaag gtggtgtttc atgtgaatca tgttcctcgt ctagataaga
     3061 ttgtatattt ttcgggaaat aaaaatcttt gtaccagaaa taaacccaaa caaatcgaca
     3121 tgcataaatt gcaatagcga ttaatatagt gatcactatt aaaaatgaaa agttgtcagg
     3181 ctctgtttgg aaaatatcta tgactacagt aggaatcata aaacctaata caacaaatac
     3241 aaaaccattt aaggcataac taaatgtgtt ccaaatttga tggtaattca tttgtagttc
     3301 cgtttgtgct ctaattaaac ggtcgcgttc taaaccatgg attagacctg cgattacaac
     3361 tgcaatgata cctgaagcat gaacttcttc tgctaaaaag tatacgacaa aaggagttaa
     3421 taattgaata aaagttaagg tattgttatc ttttaaacct ttattagcgg ttaagtctat
     3481 acgtattcta acgacaacga atccaataat tgcaccaata agtacaccta gtattgttga
     3541 aatgataaat tgttcaacag cttggaataa tgaaaaggta ccagttacta atgcagtaac
     3601 agcaatttta aatgaaatga tacctgctgc atcattgagt aaagattcac cttctaaaat
     3661 cgtcatagaa cctttaggta ataattttcc gcgtgtaata gcagatactg ctactgcatc
     3721 agtaggacat aaaattgctg ctattgcaaa agcggctggc attggtaagg caggccaaat
     3781 ccaatgtata aaatagccaa caccgactac agttgcaaac actagtgcca ttgacattaa
     3841 tagtataggt ttacgatatt ctaataattt tgttcgagag acgtgggtac cttccacaaa
     3901 aagtagtggc gcgataacgg caaacataaa tacttcagaa ttgaattgga aatcaacttg
     3961 tattggaata atgaaaataa cgacacctaa tgcaatttga ataaaggcag taggaatttg
     4021 tgggaatcga ttattgataa ccgaactaat aatcacagca aaaataaaaa ttaaaaatgc
     4081 ttctaatagt gccatacaat actcctcaaa attttaatag ttaatatttt atcactttta
     4141 aggcataatg acatagatat attgataaaa tgaagttatt ttcaaaaaaa ctctagtatc
     4201 ggttgaactg atactagagc gagatgttta aattattgat tgtcatatct gaaatgaccg
     4261 ctgtcatttt gtcgttgttc atacgcgagc ttttcagcat tcgttttgta ttttttataa
     4321 aagaaaaata aaaatataaa ccagaatggc gaaatataaa tagctgctct tgtttcgtca
     4381 ctaaagaata ataaaatgaa tacaaagaag aagaacgcta gaataatgta agcaataggc
     4441 ttaccaccaa tcaacttaaa tttactgttt ttatgtgcct caggatgctt tttcaaatac
     4501 attagataag cgacaataat cattgcccaa acaactaaaa acaacacagt tgataacgtt
     4561 gtaacgtata tgaatagttg aattgcatta gggaaaatgt agtttaacaa ggctgctata
     4621 agtaatgcaa ttgatgaaac taacattgat aaataaggca cgccatgact attcgtttta
     4681 tttaaaactt taggacctaa cccttgttgt gacagtccga ataagatacg actattcgaa
     4741 tagataccac tatttgtagc agaggccgcg gcagttagca cgacaaagtt aacgacacct
     4801 gctgcaaatg gtacgccgat taatgtaaag agtttaacga aagggctact atttggatcg
     4861 atatcattcc aaggtatgac tgacataatt actaatagac caccgatata gaataataaa
     4921 atacggatag gtacattatt aattgctttc ggtaaggttt tttcaggatc tttcgtttca
     4981 ccagcagtta cacctataag ttcaatacca atgaatgaat atacagcaat ttggaatgac
     5041 attaagaaac caaatgttcc accagggaac atgccaccgt gactgattaa gtttgtgaat
     5101 gatgcatgtc cataatgtgt tttaaatgag aaaaagatta atacaagacc aacgataacc
     5161 atcgcaataa ttgtgacaac tttaataatc gagaaccaaa actccagttc accaaataat
     5221 ctggcaccta ataagttgaa gctaatcaag attaaaacaa taaatagcac ggtaatccaa
     5281 tttgggactt gtggatacca aaatgcaaag tattgtccca tcgcagttag gtctgacata
     5341 cttgatacaa tccaacataa ccagtaagtc cagccaatga caaagccacc aaaagggcct
     5401 aaatattcat ttgcaatatc aacaaatgaa ttaaatcttg tattgctcaa caacaattcg
     5461 cctaatgcgc gcataaaagc gaatagtaca accccaataa tcatgtatgt gaataacagt
     5521 gatggaccag ttaaagatat tgtttgacct gatcctagga ataacccagt tccaattgcg
     5581 cccccaattg ctattaattg gatgtgtctg ttattcaact ctctatgcaa ttttcttgcc
     5641 ataattttcc tcctcaggat aaagcaaata aaaaagttgt gtgtaagcgt tttcttaatt
     5701 ataaatacta aagaaatcca tttaataaag caattatttt tttaaatata agaaaaaaca
     5761 tttgataggt aagggtatga taactaaaac ttatcgattt tttgaaaaga tataagtttt
     5821 tatgcaaaaa tataaaataa tcatctaaat gaagcagtta actatatata ttagataagc
     5881 tatggcataa taaataacat tttaatttga gtaaagaagg taagggtatg aagataggga
     5941 caatcgccga tttacatatt gatcgtcata ataaaaaaac atccgaagat tacttagagg
     6001 cattggttga aatagttaag tataagaaat tggatatatt gttgattgcg ggggatatct
     6061 caaatcatta tcaattgacg caccaattta taacgcaatt gacaaaacaa ttagatatac
     6121 ctgtgaaatt tgtacctgga aaccatgatt tatgggaagt tgaaagtatg actacgcaag
     6181 acatttggaa taattataag agtatgtcac agtgcttggt aggaaaacca tttatagtaa
     6241 atgaagaatg ggcaatcata ggacatactg gctggtatga ttatagcttt gcagcacaac
     6301 gattttcatt agatgagtta caaaaaggaa aacattatgg tgcgacttgg caagataaag
     6361 aacgaatatc ttggggcata tcagatcaaa atttatctaa aatagcggct gaacaagtga
     6421 agaaagatat attagaagta ggaaatagac gagtgatttt agtcacacat gttgtgacgc
     6481 accctgattt cattgttcct atgccgcatc gtatattcga tttttataat gcatttattg
     6541 ggacaagtga tttcaatcct ttgtatgcga tgttcgatat accatatagt attatgggcc
     6601 atgttcattt tcgtaaaagt gtgatagatg atggcagatg ttatctctgt ccgtgtctag
     6661 gctatccaag acaatggcgt tcagaagata tttaccagga aataaatgag acgatacaaa
     6721 taatagaaat ttaaaatgcg caaacctgac ccagtttgcg cattttatgt tttacacacg
     6781 cgagtaatgt gtttacttac gtgtgtttat tttgttgctg attttcaatt gtatatgaat
     6841 gtggttgcac ataaatgcac tttcttcctg gtgaattaaa gctgtattcc attttctctt
     6901 tacgaatttt aataatttgt ttgcgatttg gaatgatggc aggtaaaact aggccacgac
     6961 gaatatgact ccaaagtgga tcgatataat agtaatagta atcatcgtac ccaatgagta
     7021 acgttacgtg aatatttgaa acttgtttcg ctggcttgtt atcaaagcgg aaaacacgta
     7081 gtaatggttt agaaccaaga ttagtatggt atattaacac aggttgacct tgatcgataa
     7141 tacctttaag atcttctaac gatttaccag tgccgtctac gatattagga ttgtattttt
     7201 gtaaaaatgg tacatatgct tctggaaata tcgtttgatg ataattgcca agcttaatga
     7261 ataagtgatg tccaacataa cctttatgtg gattgttcgg atgtgtcggc caatgtctca
     7321 taatttctgt agcagggata tgttggttgt tgtattgcaa catcatggct gcggaaacac
     7381 cttcacaccc catgaccata gggataggaa atagctgact gataggttta actggtaata
     7441 tttttcggtt cataatatag tcctcgcatt gattcaataa atatttaata taattatata
     7501 gcgtcaatgc aaaatgtcct aaacatatgt tttacatgag tgaataaaat taatggagtg
     7561 ataaaatgga atatcaatta caacaacttg cgtcgttaac gttagtaggt attaaagaaa
     7621 cgtatgaaaa tggacgacag gctcagcaac atatagcagg gttttggcaa agatgttatc
     7681 aagagggagt aattgcggat ttacagttaa aaaataatgg tgatttagcc gggatacttg
     7741 gcttatgtat acctgaatta gacggtaaga tgtcatatat gattgcagtt accggagata
     7801 atagtgctga tattgaaaaa tatgatgtca taacattagc aagttcaaag tatatggtat
     7861 ttgaagcaca gggcgcagta cctaaagcag ttcaacaaaa aatggaagag gttcatcact
     7921 acatacatca atatcaagca gatacggtaa aatcagcacc attttttgag ttgtatcagg
     7981 atggtgatac tacaagtgaa aaatatatta cagaaatttg gatgcctgtt aaagggtgat
     8041 tgaatagaaa tgtccttgct atgaaggcgt aattttagtt gaagattcga tctcataatt
     8101 cataaagaag aaccctgaca aagatagata tctttgctag ggttctaatt atgttgaagt
     8161 gttatgcgac atgttgcttt ttaatcattt taaagtaaat gatttgtaga ctgactaata
     8221 gaatggctgt aatgaagata ataatcatat atggtgatgt attaaattcg ccttttaagc
     8281 caactaatgg tgcaacagcg ccacctaaga taaattggaa taagccaagt aaacttgatg
     8341 cgttaccact gccacctgtt cgttcttcca tagccattgt gaaaccaagc ggtccaattg
     8401 acgtcacagg acacacattt aagaagaatg cgatgagtaa gacccaaagt ggtaaatgga
     8461 atgtaagtgt caggataatt aaagcaacac ctaccacttg tataatagtt aaaatgatta
     8521 ataatatgtg gcgatgtaat ttttctacta ataaagcaac gacttgactg acaatgatta
     8581 aacctacacc gttaacagca aacatgatac taaattgttg gggtgtcata ttatacaatt
     8641 tttgagtaat aaatggcgat gcagatgaat aactaaatag cattacataa gttaaacctt
     8701 gtaatagcat tggaataata aatgctggtt ttttcaaaag acttccaaaa tctttaatga
     8761 cgctactaaa attcacctgc tttaattcat ctttagatgt tttaggtaat tgagaagcga
     8821 cgccaattaa aatgatgagt gccacaatag ttaaaattgt gaaaatagaa cgccatgttg
     8881 ctacggataa agctaatcca ccggctaatg gtgcaagaat agtgatgatg ccattaacta
     8941 ccattaaaga tgctaaaaat ttagcgagtg cgttgccact aaatttatca ccagcagagg
     9001 cttttgcaat cacgatgacg ccaccaccag ttaaaccttg aataaatcgt aaagttaaga
     9061 ataatggcaa ttgatcaaca aacatagaca aacctgatac caaaatgaaa atgatcaaaa
     9121 tggatacagc aatccgtttg cgaccaaaag catcggataa aggtccaaag ataaattggc
     9181 ctagtgcaag accaatcatt gtgaatgata atgttaattg aatttctgat gtagtagaac
     9241 caaaatcacc ttgcacttta ggtaatgatg gtacgtacat atctatagtt agtgggccaa
     9301 aagcggtcat gacgcctaac gtaattagca ataaaaatga aaaatgcttc gttttggttt
     9361 tcgttgattg catgatcaga ctcctttaaa tgatataatt atataaatta tatacgtttt
     9421 gtgtgtattt gtatgtactt agaatacaag tttatataaa aaatccctat actcttaatg
     9481 catcatcacg aataacgtag tgatacagat aaagtatagg gaattaatgt ttatcatcaa
     9541 agcaggtgta ataattaatt agcgccacca ttgtcatctt taaataagct ttctaattta
     9601 gatttaatac tgtcgaattt tgtattaata tcatttaaca tgctttcaat tttaccttgg
     9661 cttgcattgt ttttaagtgc attatttgcc tctgtcaatt tgttgatttg gtcttgataa
     9721 aattgagtta gcttagaatc agctttgttt tgttcttgct gcttcaagtt gtcgatttga
     9781 ttttgtaaat ttttaataat gtcatcagtt ttttggttgt ttttaatatt ttctttagca
     9841 ttatctactt gctggttaat ttggccgtct tgattttcaa cttgtttttt ataattttga
     9901 gagactttag ggtcattact aactttatcg ctatcattgt taagtaaggc gcgtgtgaca
     9961 aaaatagcga caataattaa gagaatggca ataatgatat ataaccattt ttttgaacgg
    10021 cgggatttct cgttttcttc ttcaagaatt tgttctcttc gataatctct accattatag
    10081 tatcgttccg ggtgttcttc aggatcgcct ttagcatatt gttgtctata cgatgcgtct
    10141 tcagattgac gacgatgatt ttggttttga ttgtcgtatt gatatgaatc acggtattta
    10201 tcagttcttt tcatgggttt cacctcattc aagttttaac tgcaaataat aaagctgatg
    10261 atttactttg acattattgt aatttcagaa tacgagcaat gtcagttgca gtgtttaata
    10321 aattttgttc gctattttgt aggacatctt ctaaagtgct aggtgaagag attatagaat
    10381 aggcactatc aataccgaaa tcgtaaacat gttgataatt ttcgcctaga ctgccacaaa
    10441 tcgcgatgac aggaatatga tattgttttg cagctaacgc aacgcctacg ggtgttttac
    10501 caaagatggt ctgataatcc atgcgtcctt ctccagtaat aacgaggtct gcatctttaa
    10561 ttctttgatg aaaatctgta atgtcaaaga cgacatcaat accttttgtt aaagttgtct
    10621 cacaaaacgc taataatgct gcgcccatac cgccagctgc accagaacct ggtatttgat
    10681 taacggactt tcctgtgcac atttttatct tatcatgata atgcgacatt gcgaaatcca
    10741 actttggtat catctttgca tcagcgcctt tttgaggacc ataaatatag gtagcaccat
    10801 tttcacccaa taaaggattt gaaacatcac aggccacttt aaaggtcacc tcttttaatc
    10861 gcgaatctag attggttata tcgatttgtg caatgtgagc aagattagca ccattcattt
    10921 gtaataagtc cccgtttaca tcagtaaact ttacgcctag tgcacttagc atacctgtac
    10981 caccatcatt tgttgcactg ccaccaatcc ctaaaataat ggtcttagca ccatgattta
    11041 atgcatcttt aattagttca ccggtaccat atgatgatgt gtataaagga ttacgttcct
    11101 ctttttctaa taaatccaaa cctgacgctg ccgccatttc aataattgca atttgttgtt
    11161 cgtctgcgcg tgcataacat gcttcaatag gtcgcattaa agggtcatta acgatgactg
    11221 tatacttagt ggcacctgtt gcatgcatta aagcatctgt ggtaccttca ccaccatcag
    11281 ccatcggaat gatatcataa tgaagggtat tcccataaac attagtaaaa gcctgtttta
    11341 taatattgcc aacttgctgt gcggtcatac tttccttaaa tgagtcagga gcgatgacaa
    11401 ttttttgtaa ttgcatagta gacatcctct cattactgtt gtcgctctta ttgtatataa
    11461 aattactagt gaactaaagt aatctctata gtgctaatga gtcacgcgat aaatcagtta
    11521 cattataatg gcgtataagg atgaggggaa agagactaaa tttgctgtga aagggtataa
    11581 agattgaaat aagcatattt gaagtgaagc ggattggctc gcatattcga gtatgttcga
    11641 gaacgtagtt atgcatatat tttgatgttg aggagttaca catatgaaac taacacaaac
    11701 gcatgctgaa attttaaaat ttatcattgt tggcggcatt aatacgttaa attattatgt
    11761 tgtctattta ttgctgttaa aattattaca cattgaatat atgattagtc atattaccgg
    11821 atttctagtc gcttttgtga tttcatatta tttgaattgt tattttgttt acagggtaaa
    11881 acctacttgg agaaaattca ttagttttcc aattacgcag attgtcaacg taagcttaca
    11941 aacagtttta ttatatgtat ttgtatcctg gctaaatttg ccagcagaaa ttgcaccatt
    12001 tgctggttta attattacga tacccatcac atttatatta tctaaatgga ttttaaaaga
    12061 tagcaattaa agtagcgtaa accactaaaa aaaactttac ttaagcctta atattgcgtg
    12121 aaatataata aataaatgat ataatatgat ggtcgcctaa catctcgggc gttactttaa
    12181 cgcagagagg aggtgtatga cggatgttca atttattaat taacatcatg acttcagcta
    12241 taagcggctg tcttgttgcg ttttttgcac attggttacg aacgcgcaac aataaaaaag
    12301 gtgacaaata accaacacct aaataaatcc cctcactatg gcagtagtga ggggatttgg
    12361 tgtatttaga tgttcaattt attatgtaat taattatagc attgatgaac aaggtgtgca
    12421 atgtattttt gcaatattac aataaatgaa tttaattata ttagaattaa gtatttcgaa
    12481 aaatgattta atagttgagt cataggatca ttagataaag cgaataatca attataggga
    12541 cattgggata caaatcaatg tactagactc tacaaggcta tattcaggga cccaacaaag
    12601 agaaattgga ttcccaattt ctagagacaa tgcaagttgg ggtgggacga cgaaataaat
    12661 tttgtgaaaa tatcatttct gtcccactcc cagaaccact acataataaa tcattagtgg
    12721 ctctttatca tttctgcccc ataaccttgt gaatttttaa aagaaatgtg tcacaaatcc
    12781 acgcgacatt gataatcgtt atcaactaat atat
//
LOCUS       DAKTIP010000062        12627 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAKTIP010000062 DAKTIP010000000
VERSION     DAKTIP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.062"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..216)
                     /locus_tag="O0305_001315"
     CDS             complement(<1..216)
                     /locus_tag="O0305_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCY0274464.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKAD"
     gene            562..2385
                     /gene="lepA"
                     /locus_tag="O0305_001316"
     CDS             562..2385
                     /gene="lepA"
                     /locus_tag="O0305_001316"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCY0274465.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            2948..4072
                     /locus_tag="O0305_001317"
     CDS             2948..4072
                     /locus_tag="O0305_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCY0274466.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            4172..5149
                     /gene="hrcA"
                     /locus_tag="O0305_001318"
     CDS             4172..5149
                     /gene="hrcA"
                     /locus_tag="O0305_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCY0274467.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            5181..5807
                     /gene="grpE"
                     /locus_tag="O0305_001319"
     CDS             5181..5807
                     /gene="grpE"
                     /locus_tag="O0305_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCY0274468.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            5876..7708
                     /gene="dnaK"
                     /locus_tag="O0305_001320"
     CDS             5876..7708
                     /gene="dnaK"
                     /locus_tag="O0305_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCY0274469.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            7844..8983
                     /gene="dnaJ"
                     /locus_tag="O0305_001321"
     CDS             7844..8983
                     /gene="dnaJ"
                     /locus_tag="O0305_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCY0274470.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            8987..9925
                     /gene="prmA"
                     /locus_tag="O0305_001322"
     CDS             8987..9925
                     /gene="prmA"
                     /locus_tag="O0305_001322"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCY0274471.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            9927..10679
                     /locus_tag="O0305_001323"
     CDS             9927..10679
                     /locus_tag="O0305_001323"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCY0274472.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            10686..12032
                     /gene="mtaB"
                     /locus_tag="O0305_001324"
     CDS             10686..12032
                     /gene="mtaB"
                     /locus_tag="O0305_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCY0274473.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            12325..12501
                     /locus_tag="O0305_001325"
     CDS             12325..12501
                     /locus_tag="O0305_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCY0274474.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT         4725 a         1731 c         2431 g         3740 t
ORIGIN      
        1 gtcagctttg tttgaatgta ttaaattact ttgagcagct ttgtctacta acttaactgc
       61 taagcttact aattcatttt tattatcagc gttatttgaa acagctgttt tagcgttttt
      121 aactgctgta cgcattgcac tcttttgtga aatgttgcgt gcttcagctt tttcagttgt
      181 ttttacacgt ttaattgcag atttgatatt tgccatttct gtcacctcct aaaagtgagt
      241 tcatcttatc aaaattttat ttgattacaa caagaaatat tttaacaaaa gtctaaccgt
      301 tgtgcaatca tttatttgat ggggttagct taaaattttg cagatttaat aaattgtgtt
      361 ggaaacacaa tcaatacgtt ttataattat aagtacgtaa ttaacatatt aacatatcgc
      421 aagtatgtat ttaaataaag attgttataa tttcaaagtt catccaagat tatggcgttt
      481 gcatttacct attaaaaacg ttattatatc aaagatgcga aagataatac gggtttattt
      541 tatgaaagtg agaaggataa aatggataat gagcaacgct taaaaagaag agagaatata
      601 aggaatttct cgattatagc acatattgac cacggaaaat ctacattggc tgatagaatt
      661 ttagaaaata ccaaatcagt tgaaacaaga gatatgcaag atcagttact agattcaatg
      721 gatttagaaa gagaacgtgg tattacaatc aaattaaacg cagtacgttt aaagtacgaa
      781 gctaaagatg gaaatactta tacattccat ttaatcgata cgcctggaca cgtcgatttt
      841 acatatgaag tgtcacgttc tttggcagct tgtgagggcg cgattttagt agtagatgcg
      901 gctcaaggta tcgaagcaca aacattagca aatgtttatt tagcattaga taatgagtta
      961 gagttattgc ctgttattaa caaaattgat ttacctgctg cagaacctga acgcgtgaaa
     1021 caagaaattg aagatatgat aggtttagac caagacgatg ttgttttagc aagtgctaaa
     1081 tctaacattg gaattgaaga gatactagag aaaatagttg aagttgtgcc agctccagat
     1141 ggtgacccag aagcaccact aaaagcgtta atatttgatt ctgagtatga tccatataga
     1201 ggggtaattt catcgataag aattgtggac ggtgttgtta aagccggaga taaaattcga
     1261 atgatggcca ctggtaaaga gttcgaagta acagaagttg gaattaatac acctaagcag
     1321 cttccagttg atgaattaac agttggtgat gttggttata ttattgcaag tattaaaaat
     1381 gttgatgatt ctagggttgg tgacaccatc acattagcta gtagacctgc atcagaacca
     1441 ttgcaaggtt ataagaaaat gaatccaatg gtatattgcg gactgttccc aatagataac
     1501 aaaaattata atgatttaag agaagcatta gaaaaattac aattgaatga tgcatcatta
     1561 gaatttgagc ctgaatcgtc acaagcatta ggttttggtt atagaactgg tttcttaggt
     1621 atgttacaca tggaaataat tcaagaaaga attgaaagag aatttggtat tgaattaatt
     1681 gcaactgcac catctgtaat ttatcaatgt gttttaaggg acggttcaga agtgacggtt
     1741 gataacccag cacaaatgcc agatcgtgat aaaattgata aaatatttga gccatatgtt
     1801 cgtgcaacta tgatggttcc aaatgactat gtcggtgcag taatggaatt atgtcaacgt
     1861 aaacgtggac aatttataaa tatggactat ttagatgata ttcgtgtaaa tattgtttat
     1921 gaattacctt tagctgaagt tgtatttgat ttcttcgatc aacttaaatc taatactaaa
     1981 ggatatgcat catttgatta tgaattcatc gaaaataaag aaagtaattt agtcaagatg
     2041 gatattttat taaatggtga taaagtggat gcgctaagct tcatagttca tagagatttt
     2101 gcatatgaac gtggtaaagc attagttgaa aaacttaaaa cgttaattcc aagacagcaa
     2161 tttgaagtac ctgtacaggc tgcaatagga caaaaaattg tagcgcgtac aaatattaaa
     2221 tcaatgggta aaaacgtttt agctaaatgt tatggcggtg acataagccg taaacgtaaa
     2281 ttacttgaaa aacaaaaagc aggtaaagct aagatgaaag cagttggtaa tgttgaaatt
     2341 ccacaagatg ctttcttggc tgtattgaaa atggatgatg aataatttta aaaaatcaat
     2401 taacaattta caatgaataa agtttaataa ctaaaaagag ggagcctagg ataaattaac
     2461 gtcctgggct ttacaatgtt atattggcag ccatcgacag agttaaaatg agcttataac
     2521 aatggggccc caacacagaa gctgacgaaa agtcagctta ctataatgtg caagttgggg
     2581 tggggcccca acatagagaa tttcgaaaag aaattctaca ggcaatgcaa gttggggtgg
     2641 gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccttat gcatgagttt
     2701 tactcatgta attttatttt taaggacata ttacatctgg ctaatgtgta agagccacta
     2761 cataataaat cattagtggt tctttattat ttctatctca ctccctctaa acaagaataa
     2821 atattaaaat gaatcgatat attagacaat cattgattaa acgttaaagt taaaagtaag
     2881 aataattgca gatagtccaa caggatatag ccgattggat aaaaagtctg agaagcgggg
     2941 cattaaaatg acggtacaaa gtgcatatat acatattcca ttttgtgtaa gaatatgtac
     3001 atattgtgat ttcaataaat attttataca gaatcaacct gtagatgagt acttagatgc
     3061 actaatcaca gaaatgtcta cagcaaaata taggatctta aagaccatgt atgtaggtgg
     3121 cggcacacca acggcccttt ctattaatca gttggaaaga ttacttaaag caatacgtga
     3181 tacgtttaca atcacaggcg agtatacatt tgaagcaaat cctgatgagt taactaaaga
     3241 gaaagtccaa ctattagaga aatatggagt aaaaaggatt tcaatgggcg ttcaaacatt
     3301 caagccggag ttattgtctg ttttaggtag aacgcacaat actgaagata tttacacttc
     3361 ggtgttaaat gctaaaaacg caggtattaa atcaatcagt ttagatttaa tgtatcattt
     3421 accgaaacag acgattgaag attttgaaca aagtttagat ctagctttag atatggatat
     3481 tcaacatatt tcgagttacg gcttaatact tgaacctaaa acccaatttt ataatatgta
     3541 tagaaaaggc ttgctcaaac ttcctaatga ggatttaggt gctgacatgt atcagttgct
     3601 gatgtctaag atagaacaat ctcctttcca tcaatacgaa atatctaatt ttgcattaga
     3661 tggccatgaa tcagaacata ataaggttta ctggtttaat gaggaatatt atggatttgg
     3721 agcaggtgca agtggttatg tagatggtgt gcgttatacg aatatcaatc cagtgaatca
     3781 ttatatcaaa gctataaata aagaaagtaa agcaatttta gtatcaaata aaccttcttt
     3841 gactgagaga atggaagaag aaatgtttct tgggttgcgt ttaaatgaag gtgtgagtag
     3901 tagtaggttc aaaaagaagt ttgaccaatc tattgaaagt gtctttggtc aaacaataaa
     3961 taatttaaaa gagaaggaat taattgtaga aaagaacgat gtgattgcac ttacaaatag
     4021 agggaaagtc ataggtaatg aggtttttga agctttccta ataaatgatt aaaaaaattg
     4081 aaatttcgag tctttaacat tgacttactt tgaccaattt gataaattat aattagcact
     4141 tgagataagt gagtgctaat gaggtgaaaa catgattaca gataggcaat tgagtatatt
     4201 aaacgcaatt gttgaggatt atgttgattt tggacaaccc gttggttcta aaacactaat
     4261 tgagcgacat aacttgaatg ttagtcctgc tacaattaga aatgagatga aacagcttga
     4321 agatttaaac tatatcgaga agacacatag ttcttcaggg cgttcgccat cacaattagg
     4381 ttttaggtat tatgtcaatc gtttacttga acaaacatct catcaaaaaa caaataaatt
     4441 aagacgatta aatcaattgt tagttgagaa tcaatatgat gtatcatcag cattgacata
     4501 ttttgcagat gaattatcaa atatatctca atatacaact ttagttgttc atcctaatca
     4561 taaacaagat attatcaata atgtacactt gattcgtgct aatcctaatt tagttataat
     4621 ggttatcgta ttttcatcag gtcatgttga gcatgtacat cttgcttcag acataccgtt
     4681 taataatgac aaactaaata caatatcaaa ttttgttaca aacaaactaa ctgagtttaa
     4741 tcagaattta caagatgata ttgtctcttt tgttcaatca gagcaggaag aaatttttat
     4801 caataaattg ttaaatacga tgaataatca tatttctaat caaagtaata gcatttatat
     4861 gggtggaaag gttaaactta ttgatgcatt gaatgaaagt aatgtatctt caattcagcc
     4921 cattctacaa tatatagaat caaatagaat tgcagaatta ttacaagata tatcttcacc
     4981 aaatattaat gtaaaaattg gtaatgaaat tgatgacagc ttaagtgata tttcaattgt
     5041 aacaagtcaa tatcattttg atgaaacgtt aaaaggtcaa atcgctgtga ttggacctac
     5101 agctatgcat tatcaaaatg tcattcaatt attaaataga atttggtaat gatggattag
     5161 aaagtattgg aggacagaaa atgacaaata aagacgaatc agttgaaaaa aacactgaat
     5221 caacagttga agaaacaaac gtcaaacaaa atattgatga ttcagttgaa caagctgaag
     5281 aaagtaaagg tcatttacaa gatgaagcaa tagaagaaac gtctgacgag aatgttattg
     5341 aagaaataga tccaaaagat caaaaaatta atgaacttca acaattagca gatgaaaacg
     5401 aagagaaata tttaaggctc tacgctgagt ttgaaaatta taagcgtaga attcaaaaag
     5461 aaaatgaaat aaacaaaaca tatcaagcac aacgtgtgtt aacagatatt ttaccagcaa
     5521 tagacaatat agaacgtgca cttcaaattg aaggtgatga tgagactttt aaatctcttc
     5581 aaaaaggtgt acaaatggtg catgaaagtt tgattaacgc actaaaagat aatggtcttg
     5641 aagttattaa aactgaaggt gaagcatttg atccaaatat tcaccaagct gtagttcaag
     5701 atgataaccc tgattttgaa tctggcgaaa tcactcaaga actacaaaaa ggatacaagc
     5761 ttaaagatag agtattaaga ccatcaatgg tcaaagtaaa ccaataactt aaatttggcg
     5821 aaaagacatt gtttaaaatt aaattaattt aatgattaaa tggaggaatt ttattatgag
     5881 taaaattatt ggtatagact taggtacaac aaattcatgt gtaacagtat tagaaggcga
     5941 tgagccaaaa gtaattcaaa accctgaagg ttcacgtaca acaccatctg ttgtagcttt
     6001 caaaaatgga gaaactcaag ttggtgaagt agcaaaacgt caagctatta caaacccaaa
     6061 cactgttcaa tctattaaac gtcatatggg tactgattat aaagtagata ttgaaggtaa
     6121 atcatacaca ccacaagaaa tctcagctat gattttacaa aacttaaaaa atacagctga
     6181 aagctattta ggtgagaaag ttgacaaagc tgtaattaca gtacctgcat actttaacga
     6241 tgctgaacgt caagcaacta aagatgctgg taaaattgct ggtttagaag ttgagcgtat
     6301 cattaatgaa ccaacagctg cagcattagc atatggttta gacaaaactg ataaagatga
     6361 aaaagttctt gtttttgact taggtggcgg tacatttgac gtatctatcc tagaattagg
     6421 tgacggtgta ttcgaagtac tatcaacagc cggtgacaac aaacttggcg gtgatgattt
     6481 tgaccaagta attattgact acctagttgc agaattcaaa aaagaaaatg gcgtagactt
     6541 atctcaagat aaaatggcat tacaacgttt gaaagatgct gctgaaaaag ctaaaaaaga
     6601 cttatcaggt gtatcacaaa ctcaaatctc attaccattt atctcagctg gtgaaaacgg
     6661 tccattacac ttagaagtaa acttaactcg ttctaaattt gaagaattat cagattcatt
     6721 aattagaaga acaatggaac ctacacgcca agcaatgaaa gacgctggct taacaaactc
     6781 agatatcgat gaagttatct tagttggtgg atcaactcgt attccagcag tacaagaagc
     6841 tgtcaaaaaa gaaatcggta aagagcctaa caaaggagta aacccggacg aagtagtggc
     6901 aatgggagct gcaatccaag gtggcgttat cacaggtgac gttaaagacg tagtattatt
     6961 agacgtaaca ccactatctt taggtattga aattttaggt ggacgtatga atacgttaat
     7021 tgaacgtaac actacgattc ctacatctaa atctcaaatc tattcaacag cagtagataa
     7081 tcaaccatca gtagatgtac acgtattaca aggtgaacgt ccaatggctg cggataataa
     7141 aacacttggt agattccaat tgactgatat tccaccagct gaacgtggta aacctcaaat
     7201 tgaagtaacg tttgatatcg ataaaaacgg tattgtaaat gtaactgcaa aagacttagg
     7261 tacaaataaa gaacaaagaa ttacaattca atcaagttct tcattatcag acgaagaaat
     7321 cgaccgtatg gtaaaagatg ctgaagttaa cgctgaagca gataaaaaac gtcgtgaaga
     7381 agtagactta agaaacgaag ctgacagtct agtattccaa gttgaaaaaa ctttaactga
     7441 tttaggcgaa aatatcggtg aagaagataa aaaatctgct gaagagaaaa aagacgctct
     7501 taaaactgct ttagaaggtc aagatataga agatattaaa tctaaaaaag aagaacttga
     7561 aaaagtgatt caagaattat cagcaaaagt atatgagcaa gcggctcaac agcaacaaca
     7621 agcacaaggt gctaatgctg gtcaaaacaa tgatagtact gtagaagatg ctgaatttaa
     7681 agaagtaaaa gacgacgaca aaaaataatt aaacaataca attagaatgt tgtcatgatc
     7741 tgaaaagtca aagtcaatag aacattggct ttggcttttt atttggatag tatgacatat
     7801 tacgttaaac tatatgagtt aattaagaaa aggagagata gctgtggcca aaagagatta
     7861 ttatgaagtt ttaggcatta gtaaggatgc ttcaaaagat gaaatcaaaa aagcgtatcg
     7921 aaagctttcg aaaaaatatc atccagatat taacaaagaa gaaggtgcag atgaaaagtt
     7981 taaagaaatt tctgaagcct atgaagtttt aagtgatgat aataaacgcg caagctatga
     8041 tcaatttgga catgatggtc cacaaggttt tggtggacaa ggattcaatg gctctgactt
     8101 tggcggtttt agtggcttcg gtggcggcgg ttttgaagat atctttagct ctttcttcgg
     8161 tggcggcaga caaagagatc caaatgcacc gcaaaaaggt gatgatcttc agtatacaat
     8221 gacattaaca tttgaagaag cggtatttgg tacaacaaaa gaaatttcaa tccgtaaaga
     8281 tgtaacatgc gaaacatgtc atggtgatgg tgcaaagcct ggaacaagta aaaagacttg
     8341 tagttactgt aatggcgctg gtcatgtagc tgtagaacaa aacacaattt taggtagagt
     8401 tcgtactgaa caagtttgtc ctaaatgtaa tggaagtggt caagaatttg aagaagcttg
     8461 tccaacatgt cacggaaaag gtactgaaaa taaaacagtt aaattagaag tgaaagtacc
     8521 tgaaggtgtc gacaatgaac aacaaattag attagctggt gaaggctctc caggcgtgaa
     8581 tggaggacct gctggtgatt tatacgtagt atttagagtt aaaccatctg aaacatttaa
     8641 acgtgatggt gatgatattt attacaaatt aaatgttagc ttcccacaag cagctttagg
     8701 cgatgaaata aaaataccaa cattaaataa tgaagttatg ttaacaatcc ctgctggtac
     8761 acaaacaggt aagcaattcc gcttaaaaga aaagggtata aaaaatgttc atggctatgg
     8821 atacggagat ttatatgttg atattaaagt agtaacgcct actaaattaa cagatagaca
     8881 aaaagaacta atgaaagaat ttgcccaatt aaatggtgaa gaaattaatg aacaaccttc
     8941 gaattttaaa gatagagcaa aaagattctt taagggagaa taaagtatga actggacaga
     9001 gctttcaatt attattaatc atgaagcagt agaattggct accaatatac ttgaaaatca
     9061 tggatcaaat ggtgtcgtga tagaagattc agatgattta attaaccaac cagaagataa
     9121 atacggtgaa atttacgctt tgaaaaaaga ggattatcca gataagggag taagattgaa
     9181 agcctatttt aatgaaatga cttatgatga taagttgcga cagcaaatta aagatgagtt
     9241 attaaattta gatgaacttg atcaacataa cgttcaattc agtgagcaaa ttattgcaga
     9301 gacggattgg gaaaatgaat ggaaaaacta tttccatcca ttccgagcgt cgaagaagtt
     9361 cacaatagtt cctagttggg aaacatatgc taaagaagcg gatgaagagc tttgcattga
     9421 gctcgaccca ggtatggctt ttggaacagg tgatcatccg actacaagta tgtgtttgaa
     9481 ggcaatagaa acatatgtat tgccacagca ttcagtaatt gatgttggta ctggctcagg
     9541 tatattaagt attgcaagtc atctaatcgg tgtaaaacgt attaaagcgt tggatattga
     9601 tgaaatggca gtgagtgtag ctaaagaaaa cttcagaaga aatcattgtg aaacgttaat
     9661 tgaagctgtt ccaggtaact tattgaaaga cgaaacagaa aaatttgata ttgtaatagc
     9721 aaatatttta gcgcatatta ttgatgaaat gattgaagat gcttataata ctctaaatga
     9781 aggcggttat tttattactt ctggtattat aaaagagaag tatgaaggta tacagtcaca
     9841 tatggagcgt gtaggtttta aaattatttc agaacaacat gacaacggtt gggtttgtct
     9901 tgttggccag aaagtgagtg aataatgtgc aacgttattt catagaccaa aacgctgatg
     9961 taagtcagcg tttttttatt acaaaaaaag aagatattca tcatataaca aatgtaatga
    10021 gatatgatgt tggtagtaaa attattttaa cttttaatga tcaaacagtt tatttatgtg
    10081 aaattgctga cataacaagt gataaaattg aagttgattt aaaagaaaaa caaaatatta
    10141 atacagaatt gccagttgat gttacgattt gcagtggact aatcaaagct gacaaatatg
    10201 agtggttgct acaaaaagct actgaattgg gtgcttcatc atttatagct gtgagcatgg
    10261 aacgttcaat tgttaaatta aacgaggcaa aagttgccaa aaagatagag cgttggcaaa
    10321 aaataattaa agaagctgct gaacaaagct atcgtttagt gattccatct attcaattcg
    10381 agtcgaattt aaaattaatt tgtgatacta ttgataatta tgactatatt cttattgcat
    10441 atgaagagga agcaaaagat ggtgagttaa gcaatttcaa gcaaacttta caacaattca
    10501 atgctcagga taaagtgttg atgatttttg gtcctgaggg tggtttgtca gaaaatgaaa
    10561 tttcattatt tagtaatact agtacagttg ttggtcttgg gccgagaatt ttacgggcag
    10621 aaactgcgcc attgtatgcg ttaagtgcaa taagttatga aaaagaatta atggggtgaa
    10681 tatttatgtc aacagttgcg tttcacacat taggttgtaa agtaaaccat tatgaaactg
    10741 aagcaatctg gcaattattt aaagaagcaa actatgagcg cgttgacttt gaagcgaatg
    10801 ctgatgtatt tgttattaat acttgtacag taacgaatac gggtgataaa aaaagtcgtc
    10861 aaataattag acgtgcaata agacaaaatc ctgatgctgt aatctgtgta acaggttgtt
    10921 atgcgcaaac ttcatcagct gaaattatgg aaattcctgg tgtcgatgta gtagttggta
    10981 cacaagatag acataaacta ttaggttaca ttgacgaatt ccgtaaagaa cgccaaccaa
    11041 ttaatggtgt tggaaatatc atgaaaaatc gtaaatatga agaattagat gtcccttatt
    11101 ttacagatag aacacgtgcg tcattaaaaa ttcaagaagg ttgtaacaac ttctgcacat
    11161 tctgtattat tccatgggct cgtggcttaa tgcgttcaag agatccggaa aaagtagttg
    11221 aacaagcgac gcaactagtg aattcaggat ataaggaaat tgtattgacg ggaattcata
    11281 caggtggata tggtcaagat ttaaaagatt ataacttggc ccaattatta cgtgatcttg
    11341 aaacgattaa tggattagaa cgaattcgaa tttcttcaat tgaagcaagt caacttacag
    11401 atgaagtaat tgacgtttta gaacgttcaa caaaagttgt gcgtcatttg catattccat
    11461 tacaatctgg ttcagataca gtattaaaac gtatgagacg taagtataca atggatagat
    11521 tttcagaacg attaacaaaa ttgcataaag ctttaccaga cttggcagtt acgagtgatg
    11581 taattgttgg tttcccaggt gaaactgaag ctgagttcca agaaacatat gattttatcg
    11641 taaagcataa gttctctgaa ctgcatgttt tcccttattc tcctagaatt ggcacgccag
    11701 ctgcaagaat ggatgaccaa attgatgaag aaattaaaaa tgaacgggtg cataagttaa
    11761 ttacgctaag caatcaactc ggaaagttat atgcgtctaa atttgatcaa gatgtgcttg
    11821 aagtaattcc tgaggaacag ggtgacacag aaggtacatt agttggatat gcagataatt
    11881 atatgaaagt acaatttgaa ggtgacgaat cactcatagg tcaaattgta aaagttaaaa
    11941 ttacgcaagc aaattatcca ttaaatgaag ggcaagcaat taaagttgtt gatttcgcaa
    12001 caaataaatc tgatagagaa gttttagttt aagtattcct tgattcatac catatggttc
    12061 cgtattaaag atataggttt tgtaacgttg ttttaacggc ttcacaattt actatataag
    12121 caactcttct cattaaataa attattaaga gatttttaga acaaaatcta aaagtctttg
    12181 tgattttgat gtgaaatatg tttcaaataa aaaacaattt actaattgac cataaattac
    12241 agatatatta tacttataaa tgcatagttt tactgtgcaa ttgactataa agttccgttg
    12301 atatttggag ggagggaaat acagatgtct aaaacagtag tacgtaaaaa tgaatcactt
    12361 gaagatgcgt tacgtagatt taaacgttca gtttctaaaa gtggaacaat ccaagaagta
    12421 cgtaaacgtg aattttacga aaaaccaagc gtaaaacgta aaaagaaatc agaagctgca
    12481 cgtaaacgta aattcaaata attaatacct ctgttgactc cctcaacacg aatattaatt
    12541 atataaaaca aacatcacaa gttagtgtct gacactaata tgtgatgttt ttttgttgtc
    12601 aatttttaat taaaaaaagt tatatag
//
LOCUS       DAKTIP010000063        12341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAKTIP010000063 DAKTIP010000000
VERSION     DAKTIP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.063"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..123)
                     /locus_tag="O0305_001326"
     CDS             complement(<1..123)
                     /locus_tag="O0305_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HCY0274475.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVH"
     gene            complement(330..1607)
                     /locus_tag="O0305_001327"
     CDS             complement(330..1607)
                     /locus_tag="O0305_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCY0274476.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQRFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            1768..2391
                     /locus_tag="O0305_001328"
     CDS             1768..2391
                     /locus_tag="O0305_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0274477.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGVPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            2437..3381
                     /locus_tag="O0305_001329"
     CDS             2437..3381
                     /locus_tag="O0305_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCY0274478.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(3467..4909)
                     /locus_tag="O0305_001330"
     CDS             complement(3467..4909)
                     /locus_tag="O0305_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCY0274479.1"
                     /translation="MNYKQSAEEILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            5092..5475
                     /locus_tag="O0305_001331"
     CDS             5092..5475
                     /locus_tag="O0305_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCY0274480.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            6963..9068
                     /gene="rsp"
                     /locus_tag="O0305_001332"
     CDS             6963..9068
                     /gene="rsp"
                     /locus_tag="O0305_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCY0274481.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            9139..9561
                     /locus_tag="O0305_001333"
     CDS             9139..9561
                     /locus_tag="O0305_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCY0274482.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            9826..10182
                     /locus_tag="O0305_001334"
     CDS             9826..10182
                     /locus_tag="O0305_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0274483.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     TTIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(10586..11863)
                     /locus_tag="O0305_001335"
     CDS             complement(10586..11863)
                     /locus_tag="O0305_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCY0274484.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(12045..>12341)
                     /locus_tag="O0305_001336"
     CDS             complement(12045..>12341)
                     /locus_tag="O0305_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCY0274485.1"
                     /translation="FFFGLKTYQGHKNIQLIDSYLEEKNLKDKIKSEKTEYSAKKGLF
                     YKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETKKSLKTAKHKYFNQNYKPSK"
BASE COUNT         4288 a         2014 c         1786 g         4253 t
ORIGIN      
        1 gtgtacaagt gataattgtg ttgtaccagt aaaatcatat gcttttgtta gctctctata
       61 gaaaaagcca ctaaataaac ctatgatcat ataaaaaaga aatgaatata ataacctacg
      121 cattttgaat cctccctcac cttttcatta tatccgatta tacctattat aacagtgaca
      181 aaaaagcgaa gatatgacac aactgaattt ggtttagcac atagtatgac agctaaaata
      241 actttacatc aagcgctata caaataaaat agctatacca tgacgctcgc tacaaacgtc
      301 ttgtatagct attatttcta tttattaaat tatgacggca cttcagtacg ttttgtgcta
      361 tgttttctaa caatagctga aactaaactt gatacagcgc caaaaatcat aaaccctata
      421 aacatccaca ttgtactatt taattcaata tgatgattca agtataatag ttctcttaca
      481 cttgttgcat agtgtgtgaa tggattccaa tcatatacat atttttgata actttcaggc
      541 aacatttgtt taggtaacgt tacaagttgc atactaaaga acattaaaat gaagaaaatt
      601 ggaactgact tcatacctag ccataccata acacctaaaa taaggcccac gaaaccaaga
      661 atcgcaaatg ctacaaaaat tgcgatacga tttggatggt caaaatcaaa tctttgaacg
      721 ccttgcatga aataaatata aacaaagcta cctgcaaatg ctgcaacaac tgcaaatatc
      781 atctgtccaa ttgaagcaat gatacgatgt tgcacgacga tattgttact agttctaaat
      841 gcaaagaaca ataagataga cgttacgatt gaacctatcc aaattggcat aaacattagg
      901 aatggtgcat taccacctgc ttggtgatct ttaactttat taagtttttc attatccact
      961 tttactggat tcgtcaaacc attgatgtcc gcggcattga ctttaacatt ttgactcgtt
     1021 aatgtttcca aactttgctt cgtaatttga gcattaatat tatcacccat accagttaac
     1081 acagctgatg caatttgtga accttgtaag cttgatcctt cacttacaat cgttttaaat
     1141 ttagcctgct taacctctac ttgttgattc cccatttttt gtttcatttg ttgaaccact
     1201 tgtggcggaa tttcacctga agcaactttt tgttgcattt cttctttttt actatccata
     1261 actacttttt gtgttttact catagcatct tttgaaaagt ctttatcaat aatagctact
     1321 ccaaagattt tttgatcttt caaatctttt tcaaggtctt tttcactatc aaccttaacc
     1381 catttaattt tattagaatc actatctaat aatttatcct ctaatttttt accaatgtta
     1441 acatttttat cttgaatcgt tgtaccttta tcctcgttta atataccaat tggtaaatct
     1501 tttggtttag gattatatgc aggataaaaa gctaaagaaa agattaccaa gataatcata
     1561 gttgctattg gtgcaatcca taataattta tttttaaata tattcatttt attaattacc
     1621 tccttattag acaatgtgtt gaatatttcg catgttgtct attataatat gtaagtggga
     1681 ataaacaata cgcaatgttc atcaatacgt tcattaatag acaaatacac ccacattgtt
     1741 caataacaca aggaggttga tttatttttg aaagaagata ggcgaattag aaagacaaag
     1801 tcctccatta aacaagcgtt cactaaatta ctacaagaaa aagatttaga aaagattacc
     1861 attcgcgaca taacaacacg cgctgatatc aatagaggga cattttactt acattacgag
     1921 gataaatata tgttactcgc agatatggaa gatgagtata tttcagaact aacgacatat
     1981 actcaatttg atttgttacg cggttcttca attgaagaca ttgcgaatac ttttgtaaat
     2041 aatatactca aaaatatttt ccaacatatt catgataatt tagagtttta tcatactatt
     2101 ttacaattgg aacgcacgag tcaattagaa ttgaaaatca acgaacatat taaaaataat
     2161 atgcaacgtt acattagtat caatcattct atcggaggcg ttccagagat gtatttttac
     2221 agttacgttt ccggagcaac aatttcaatt attaaatact gggtaatgga caaacagccc
     2281 atttcagttg atgaattagc taaacatgtg cataatatta tttttaatgg tccattaaga
     2341 ataatggcag aaaaccgttt gcataaatca aaccttgatt cactaactta agtaatactc
     2401 gcttttccta aatcctttag aaaggaacta tatatcatga ttacttcatt cagacattct
     2461 gaagatatag ataaacatat tataaaaaca ccattagatc atacagcgtc atggattaat
     2521 gtagtagaac cagaccgaga agaaattgaa aatcttatgg aacaatataa tatacctgaa
     2581 gactttatac gtgacccttt ggactcagaa gaaagttccc gtattgaata tgacgaagat
     2641 actggttact cattaatcat tattgattta cctatcgtca attcaactaa tcgtagcgtt
     2701 ctatcttttg taacgattcc attaggcatt attattggca acggtattat cgtaacagtt
     2761 tgtgacgctg aaaatgaatt tttagaaaat ttacctaagc gtgatattaa tttaaaattt
     2821 cacagcagat ttgcattaga aattttaact actattgccg accattataa tcgtaactta
     2881 cgattactta ataaaagtag aattcgtatt gagaaagaac taaaaaataa cattactaac
     2941 aaacaacttt tcaaattaat ggaagttgaa aaaagtttag tatacttttt agctgcctta
     3001 aaaggtaacg atacaattat taaaaagtta ttccgtttac ctgcaattaa acgttttgaa
     3061 gaagatgaag aattactaga agatttaatc attgaaaata accaagccat cgaaacaaca
     3121 gaattacatc aacgtatctt agaaagtatc acaacatcat acgcttcctt attatctaat
     3181 gatatgaata cgattatgaa gacattaaca cttttcacgg tactattaac gttaccaaca
     3241 ctcgtattta gtttcttcgg tatgaatgtg ccgttgccaa ttgatgacca tagttatata
     3301 tcttggatta ttgtggtagg tatttcatta attcttgtcg taattgttag tattttctta
     3361 tggcgtaaac agaaattata actaaattaa aaggtatgac agcttaaagt ttcatcctat
     3421 tgcatttgca atgactgata ctttgattac tgtcatacct ctttttttat tctacaactt
     3481 ctttatttgc ttttctttta gataaaatta aagtaactgt tatagcaatg atgaatgata
     3541 ttgtcatacc aacaaagtag tgtaaccatc ctgcatgtac tggattgatt gaaataaatc
     3601 ctggcaatcc agcagttcct aatgcgattg ctttaacctt gaagaaagca atatatgctg
     3661 aaccaatacc tgatccaacg atagcgccaa taaatggata tcttagtttt aagttaacac
     3721 caaacatagc cggttctgta ataccaagta atgctgaaat acctgcggca gatgccacac
     3781 cttttaactt cttattttgt ttaataataa agaacgctgc aattgctgca ccaccttgtg
     3841 caacattaga cattgtcgca atcgggaata taaatgatcc acccgtttta gtggcatctg
     3901 caattaatgt cgtttctaca gctataaagc tatgatgcat acctgtaata acaatcggag
     3961 catacaataa tccgaatatt aatccaccaa ttgcaccacc aaattcataa agccatgtta
     4021 aaccatctga taaccagtaa cctaattgtc gagtgattgg acctacaaat gaaaatgtta
     4081 gaaatgctgt tataaaaata gataacaatg gtgttaacaa attatctaac accgttggaa
     4141 taactttgcg taaccctttt tcaattgagg ctaagatata agctgctaca agcataggta
     4201 acacttgtcc ttgataacct acttgattaa tatgcaaacc aaaaacatcc caatatggaa
     4261 tagccttacc tgcttcaact gcttttggga aatcgtatgc gctcattagc gatggatgaa
     4321 ctagtatcat acctaatgca gcacctaaaa atggattgcc accaaagcgt tttgctgcac
     4381 taaatccaat taaaattggt aataatgtaa atggtgcatt cgcaaaaaca tttatcattt
     4441 cagctaatcc agcaaattga ctatatacat ctatcaatga tttacctgaa aagaataaat
     4501 ctttcgcagt caaaatgtta tttaacccca ttaataaacc accagcaacg atggctggta
     4561 taatcggaac aaagatatct gaaagcattt ttacaaatcg ctgtaacgga ttcatatttt
     4621 tagcagattg tgctttgact tccgaagtgg ttgatgcttc ttttccagtt aatttttcca
     4681 gttcactaaa tactttattg actgtaccag acccaataat aatttggtat tgtcccccag
     4741 tagaaaacgt ccctttaact acatccatat tgtttagcgc ctcttcattt actaaacttt
     4801 catcatttaa aactaatcgt agtctcgttg cacaatgcgc cattgcatct aaattctctt
     4861 ctccgcctat cgcgttcaaa atttcttctg cggattgctt ataattcata tatttagcct
     4921 cctaattaga accggttcca tttattccac tttattgtaa ccggttccaa aaatatttgc
     4981 aacactctat tatcatttta tataaacacc tacaccattt ttgatagcgc attcattttt
     5041 attgctatac tacatgcatg tatattaaca ttaattatcg gaggtaattt tatgaaagaa
     5101 cgtggctata tcgaacagct ttggcgtgag gaaaaatatc atgtattgtt acacagtcaa
     5161 cagtcttacc aaatgataag gaatgcttta aaaacggatt tatcacttca tcaagttcaa
     5221 caaatgattg atgttgcttt gttaattgaa ccgagtatag gaagtgtatg taatgctttt
     5281 gatcatatgt ggggatattt aaaaaaatgt gcgaatgaag aagaaagaca acaatcaaaa
     5341 ctacttaaag ctgattttat taatggtaaa atcgacaccc aaactttatt agatttttta
     5401 gcagaacttg ctaataaata tgatgttcaa tatttactac aaagtcgtgt tttaaacaca
     5461 aaaagaaaga gatagattga aactttagtt gtaacggaca aatagtagtc attggagttc
     5521 agcaattcat actttgtact aaagtttact ataatctatc tctcaaggtt taaatgtaca
     5581 tatctatatt aaaatgcgtt tcatgatgtt agaagatacc atacacattt aattagttac
     5641 agcataaatc aatttatacc cttaattatt acattgttta tattttaaat tcgggggagt
     5701 aattttaagt aatatcttgt tgctgctaat tctattgtta ataaattgat attagatgta
     5761 gaaattgagt gtgaaagtta ataatagatg atttagctag taacataatt aattcatata
     5821 tagtcaaaaa gttctgttag atactgttct tgatgaatat tctttgatgt taggtgttag
     5881 ctattctaga tgagtttctt agatgtggat gcctgtcttg atgaagttat ctatgatgtt
     5941 aatataatac ttgatgttga ttcttagatg ttagtgcgat ttcaagatgc tgattctttg
     6001 ttgttgcact atcttggtga cgtttctagt tgtttatagt tatcgcatct cttgatgtaa
     6061 aatctaagat gtttatgcat ttctagatgt taattcttcg ctgcttaaga attatcttga
     6121 tggtaaatct agttgtgcca tgcattttct catgatgaat ctaaggtgtt aattcattga
     6181 tgtgtgctgt ttcttgttgc ttcattatct tgatggtgaa tttcgttgtc taatgcactt
     6241 tttcaaatga ttattctaat tgttacagca ataactagat ggattttctt agatgttcac
     6301 ttttctagat gtttatactt atcttgatgt tttctaaaca gaacttttaa acgctttatt
     6361 ttttagtaga ttgtttaaaa tttcttagtt gatggttatt gttgttgatt tgaataattt
     6421 gtagcgaata ttttgtaaca gggctactaa gaatttttat aacaagaagt gatttcaatc
     6481 gtactaattt tcagcgtttg atgacaaaag tgtatgtcca aacttaaata atggcgataa
     6541 aatttaagtc atgggtacat ttaatgtttt ttgattcaaa cttccatttt ggtcaatttg
     6601 ctttttttat aacatagttg aatatcaata aaagattagc aagttagttc aaaatggcta
     6661 tgtattgttt tttgaaatac ataggtttga aatctatatt aattatgcac caaataattt
     6721 aattagactc tatcgaaaat ttccaaattt tgcttactat ctttctaatt ttacttttat
     6781 attgttcgca atgcataaaa acaagcgttt tcctaggagt tttcattaat ttcttttatt
     6841 tttcactatt accttttcaa aaatattcaa ctataatagg tatactatcc atgattttaa
     6901 aaatttccat gttttacatt taaatgatag atttcaatat atattaggca ggagagagaa
     6961 atatgacatg ccaacttaaa atacatcata cattatcgac aataccaagt cgtaatatta
     7021 ataatattat ggtgctattt tcacttacaa gtaaactcaa tattacgatt aatggtgaaa
     7081 ccaaagacgt aagtaattat attatcttaa ttaaccatgg tgatatttat aatattaacc
     7141 acggcgaaaa tataatcgaa ttaatgatac ccgtttttta tttttatcaa caagatgatg
     7201 atttctttaa tggttattta gatcgtcatt tattacaatc aagtaattat attaagtcat
     7261 taattgccga tttaatcagt acaccaacaa gttcttcttt aatggggaaa aatataggtc
     7321 aaagtattat agacacttta cttaaagaag cttttataag aatagatcat gaatatttac
     7381 caaatattgc tttaagcaat ccggtgttca tagactgtgt aaactatatt catgacaata
     7441 ttgatgcgca cttgtcttta aaagatatag cgatgcattg caacatctct gaatcatatt
     7501 gttctaatct ttttgttcga tatttgagta tgaattttaa agattatttt acaagtatta
     7561 aacttgttaa cgccatcaat ttattacttt ctacaaaaca ttctattaca actgtttctg
     7621 aattggccgg ttttaacagt catacaaatt ttgcaaatca atttaagaat tacttgcatt
     7681 ttagtcctaa acagttccgt tcactcgttt ccaagattac tgaaccacca caaatacact
     7741 tccaacaaga taacgtatca caattcactg agttaatttc aacaattgat ttaacagctc
     7801 aactggctac aaatacaaca gatattcata tagatgattt taatccgaaa gatcgaagtc
     7861 agcgtgccaa agtatttgtt cgatttagta atttcaatga actatttcaa tttattttca
     7921 atgaatatta cgatattaac tttgagcact taccaaaacc tgtcgttttc attgacgata
     7981 ttcacgatat tgagattagc caaacgaatt acaacctgtt aaatagatgt tttgaaaagc
     8041 ttttcgaaaa aaatataggt ttagcaatcg ctattaaatc aacacaacaa ttcgaaacga
     8101 tgaagcaatt aatactaaca tttttacaag gcaatcaaga ctataagaca agcaagaaat
     8161 tagttaaatt tatgctcgta ttttgctcta attctatgac agcagaagaa attcatttat
     8221 gtcatcttaa aattaaaaac aaaaataaag aaattaaata tagtgtgaca gtcgatggct
     8281 ttttagaaac ttattcaacc gtggaacaag tttatgatgt catgcagcgt cttaaattcc
     8341 attattactt tattgatatt gaaaattcaa aaactgcaac acatcttatc actaaaaacc
     8401 aacactatca tcaaactgac actcattttg aacagtataa gaaatttatt ttagattcag
     8461 gtatatcatc aacacaattt gtatataata acctgtctgt aagcggattt aaatatacta
     8521 atgatggtaa gaatccaatt caattatctg acatagtgta tcacttaatc gcattattac
     8581 gatatggcgg tggcattagc tatcaattat tagatgacca ttcaaattat atttccttgt
     8641 acaacaaata tggtagcccc ctaccattaa tgcatttata taaaatgttt agaccttttg
     8701 ttaatgaaga tattgaaatt acaaataatt atgtattgag tcgtaaagat aataactacc
     8761 atttcttatt attcaataaa attaatgatc gatatatgtc agacgtaaaa caagatttca
     8821 ttttccataa tgaattacct caagactctt tgatgattat taaaacattg aatcatgaac
     8881 atggttcaat tcaacatttg cttccaataa gcgatcaact tgtttatata gaaaaagaaa
     8941 ttttagatga attagacaaa acgaattacc ctaaaacgga gcttgcagtt caagaagaaa
     9001 ctggtcgtac atttgaactc aagttaaatc acgacgaggt taaatatatt tgctttaaac
     9061 caagctaaat actaacagtc ctcttgtgtt tagtttctta cgttaaaggc tatttatatc
     9121 ataaggagat gatttgtaat gagtaactca caagcaattc aagcaattga aaacgtgtta
     9181 gcaacgtcaa aagttggtgt attatcaact gcatataata ataaacctaa tagtagatat
     9241 atggtctttt ataatgatgg tcttacttta tatactaaaa cgaatatcca ttctgctaag
     9301 gtcaaagaaa ttaaagataa tccagcagca tatgttttgt taggctataa cgacacaact
     9361 aatcgtagtt tcgttgagat ggaagcgaca atcgaaatcg ttacagaaca agaagtgatt
     9421 gattggctat gggaaacaca agacaaaagc tttttcagtt caaaagaaga tccagagtta
     9481 tgtgttttaa gagtagttcc gcaatccatt aagctaatga atgataaatc attagataca
     9541 cctatcaaaa tcgatttata acacaaagtg tatataggaa ataactttta tgaattctag
     9601 atataacaat gttaaatact taaaataact cgctataatt aaagtgttta atatgtttac
     9661 aattcaattt gcaatgtatt gttttttaga cacactttat tcacatttaa cgtgattagg
     9721 gtgtgtcttt ttaaattaat tttatgtaat ataaatactg catttgcaaa ctgttgcact
     9781 tttaggtata acagaattaa ctacatttaa ggagattgat gaaccatgaa aaagaaaaaa
     9841 ggttttggtc ttggtattag tttaatcgcc atcatgttaa ttgtatgtat tgtattagta
     9901 atcatgatga tgactggcgg aaagaaagat acatactatg gaattatgaa agataatact
     9961 actattgaaa aaatgattag tgaaaaagat gaaagtattg aaaaaaatgt taaattacct
    10021 tcagattcag atgttaaagt taaaaaaggt gattttgtaa ttgtttataa attagcagat
    10081 tcagataaaa ttgttaaagt taaaaaagtt gaccatgacg atgtaccaca tggtttaatg
    10141 atgaaaattc atgacatggg caaaatgcac atgaaacact aattgtaatt taaattacaa
    10201 attttagttg ccatcaaggt atatacgagt aaaagcagcg gtaagttgat ttccaatttg
    10261 gaatcatttt actgctgctt tttatatttg aaatactttc atattgaata gctccacttg
    10321 ccgttcgcct gcgctttgcg catgcataaa agcccctaac aacctgaggt cactgcgctc
    10381 cggttcgcct gcgctttagc gcatgcataa aagcccctaa caacctgagg tcactacgct
    10441 tcggttcgcc tgcgctttag cgcatgcata aaagtcccta acaacctgag gtcactacgc
    10501 ttccgttcgc ctgcgcttta gcgcatgcat aaaagcccct aacaaccaaa ggttgtaagg
    10561 ggctttaaat gtcagatagt attttttaaa ttgattttaa atattcttga ccttttgctt
    10621 tgtcatatac gttttcccat ttagctataa cgattgttga taatgcatta ccaataacgt
    10681 ttacacatgt acgtaccata tctaagatac ggtcaacacc aataattaat gctaaacctt
    10741 gtgccggtaa gcccatggca cctaatgttg ttaataaaac aacgattgaa gttcctggta
    10801 ctgccgccat acctttagac gtaatcatta atgttaacat caacacaatt tgctctgata
    10861 atgttaagtg cattccatac atctgtgcaa cgaataatgc tgcaatagat tgataaagtg
    10921 ctgatccatc taagttaaac gtataaccaa ttggtataac aaaagaagta atttcttttg
    10981 gagaaccgaa gttttccatt ttcttcatca ttacaggaag tacagcttcc gaacttgatg
    11041 tagaaaatgc taaaagcaat tcacttttca agattctaat aatattcatg atattaatac
    11101 cacacatcca tgcaactagt cctagtatag cgaatacaaa gaacaccatt gcaaagacaa
    11161 caactaatac taattttaat agtggtaata atgcggatgc accaaatgta ataattgtag
    11221 tacaaatgaa tgcaaacact ccaagtggtg ctaattttaa aattttatta atcatccaga
    11281 acacagcttc aagcgatccg cttaaaaatt ctttaactgg ttctgctttt ttacctacag
    11341 cagctaatcc taatccaaag aatactgcga agaagataat aggtaataat tcacccttat
    11401 ttaaagcttc aaaaaagttt gtcggaataa tatgtacaat ggtatcaata aaatgatttc
    11461 catatgtaga ttgttctgct gcatgtgcag ttgattgata tttagaaata tcacctttag
    11521 gtaattttgt tgggtctaat ccagcacctg gtttaaatag gttaccgaag ataatcccta
    11581 aacctattgc gattgttgta ataatttcaa agtataaaat tgttttccag ccataacgcc
    11641 ctacagtttt cgattcccca acgttcgaaa tagaaagcgc tagtgagcaa aatacaactg
    11701 gtataacgat cattttaatt aaatttaaaa atacgtcacc aaatggttta atgtaatttg
    11761 ctacgttttc ttgtccatat aataaaagtc ctacgacaac acctaacact aatgcaataa
    11821 caacttgcat aggtaggcta atttttctct tgaatagagc catagtacaa actccccttc
    11881 atctgacatc actcaattat atcagaaaat tgataattat ctataaaaaa ttaaaatatt
    11941 tatcttttat agatgtcaaa aatcactaat ttccatacaa aaactgttta cctcaaataa
    12001 ttaaaattga aggcactaaa ctgcttcacc agtttaacgt ttcactattt tgatggttta
    12061 taattttgat tgaaatattt atgctttgca gttttcaaac ttttcttagt ctctgtatca
    12121 aagccttcaa caaataatcc tttattagta ctgattggtt gcacaacata agttacacca
    12181 ggttcatctt taaatgtaac ttccttatag aagagacctt ttttagcact atactctgtt
    12241 ttttctgatt taattttatc ttttagattt ttctcttcca agtaactgtc aattagttga
    12301 atattcttat ggccttgata tgttttcaga ccaaagaaaa a
//
LOCUS       DAKTIP010000064        12298 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAKTIP010000064 DAKTIP010000000
VERSION     DAKTIP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12298
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.064"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0305_001337"
     CDS             complement(177..1079)
                     /gene="cvfB"
                     /locus_tag="O0305_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCY0274486.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1226..2827
                     /locus_tag="O0305_001338"
     CDS             1226..2827
                     /locus_tag="O0305_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0274487.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            3687..4892
                     /locus_tag="O0305_001339"
     CDS             3687..4892
                     /locus_tag="O0305_001339"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0274488.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIISNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            4956..5945
                     /locus_tag="O0305_001340"
     CDS             4956..5945
                     /locus_tag="O0305_001340"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCY0274489.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            5947..6834
                     /locus_tag="O0305_001341"
     CDS             5947..6834
                     /locus_tag="O0305_001341"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCY0274490.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLRETYDTFKAGENE"
     gene            6831..7553
                     /locus_tag="O0305_001342"
     CDS             6831..7553
                     /locus_tag="O0305_001342"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCY0274491.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKGADVAIDFSNPNLLFPLLDEDFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            7580..8299
                     /gene="dapD"
                     /locus_tag="O0305_001343"
     CDS             7580..8299
                     /gene="dapD"
                     /locus_tag="O0305_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCY0274492.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            8442..9593
                     /locus_tag="O0305_001344"
     CDS             8442..9593
                     /locus_tag="O0305_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0274493.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            9598..10683
                     /locus_tag="O0305_001345"
     CDS             9598..10683
                     /locus_tag="O0305_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCY0274494.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            10673..11938
                     /gene="lysA"
                     /locus_tag="O0305_001346"
     CDS             10673..11938
                     /gene="lysA"
                     /locus_tag="O0305_001346"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCY0274495.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(12178..>12298)
                     /gene="msa"
                     /locus_tag="O0305_001347"
     CDS             complement(12178..>12298)
                     /gene="msa"
                     /locus_tag="O0305_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCY0274496.1"
                     /translation="DVLITSKDFGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
BASE COUNT         4292 a         1935 c         2242 g         3829 t
ORIGIN      
        1 atcaaataat taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct
       61 attcgtttga attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct
      121 gtccgattta atattgaata aaaaaaacac gcatcctacg acacgtgtaa aaatgattat
      181 tcttttgagt ccattcgact ccaacctttt ttagttaaag cgattttacc tgtttctata
      241 ttaataatct tctgtttata taagtgaccg attgcacgtt tgaatgaacc tttactcata
      301 ttgaatactt ctttaatcgc ttcagggctt gatttgtccc agaatggtaa ttcaccatca
      361 tattcaacta gtaaatcaaa gatgacttgg ccgtcatcgt ctaaacgttc atgtgcaagt
      421 ggtaaaaatg aaccatttaa ctcaccttta tcattatgcc cgataattct aacttgaaca
      481 gattcaccta atcttggttc agctttacgt tctgattcat gtacgaaaat tttgtaacct
      541 gattcgctta ataaaaagct accaattcgt aatacgcggt aaggtttggc ttcaatgact
      601 tcgtttttta aattatcgtc gtgtacaggt gtaaacatat tttctacaac agattcactc
      661 gctaaacgtc catacatatg attctcacgg tcaattcgta atgtaactag caaataatca
      721 ccaggttgtg gccatagtga tttcactttt ggtaaatctt cccatggtac taacacttca
      781 cggggtaatc caacatctat acgtgcccca tcgcgatccg ttttaagtac tttagcaaag
      841 tcatatttat ctttcgtaat atcaggcata ttttgagttg caaataattc acctgaacgg
      901 tttggataaa tgaagaaact atattcttca cctacttcta attcatcatc atcgttcatt
      961 tctgattggt ttaactttac gttttcaccg tttggtcctt ttaaaaggta agttgaacct
     1021 tgtaacccta ctacttcaag gaattctata gaacctacta tatctttgtc taatgccatg
     1081 ttattctcct tcgattcttg tattctttat tattataaca tgtgtgagtc attctatcat
     1141 tacaacttat atgaggtcga aactgtgacg ataatatgat ataattcttc cgttagaata
     1201 tcgaatgaaa aaaaggagaa aatgcatgtt acaagtaact gatgtgagtt tacgttttgg
     1261 agatcgtaaa ctatttgaag atgtaaatat taaatttaca gaaggtaatt gttatggatt
     1321 aattggtgcg aatggtgcag gtaaatcaac attcttaaaa atattatctg gtgaattaga
     1381 ttctcaaaca ggacatgttt cattagggaa aaatgaacgt ctagctgttt taaaacagga
     1441 ccactatgct tatgaagatg aacgcgtgct tgatgttgta attaaaggtc acgaacgtct
     1501 ttatgaggtt atgaaagaaa aagatgaaat ctatatgaag ccagatttca gtgatgaaga
     1561 tggtatccgt gctgctgaac ttgaaggtga atttgcagaa atgaatggtt ggaatgctga
     1621 agctgatgct gctaaccttt tatctggttt aggtatcgat ccaactttac acgataaaaa
     1681 aatggctgaa ttagaaaaca accaaaaaat taaagtatta ttagcgcaaa gtttattcgg
     1741 tgaaccagac gtactattac tggatgagcc tactaacggt ctcgatattc cagcaatcag
     1801 ttggttagaa gatttcttaa ttaactttga taatactgtt atcgtagtat cgcatgaccg
     1861 tcatttctta aataatgtat gtactcatat cgctgattta gacttcggta aaattaaagt
     1921 ttatgttggt aactatgatt tttggtatca atctagtcag ttagctcaaa agatggctca
     1981 agaacaaaac aagaaaaaag aagaaaaaat gaaagagtta caggacttta ttgctcgttt
     2041 ctcagctaac gcttctaaat ctaaacaagc aacaagtcgt aaaaaacaac tcgagaaaat
     2101 tgaattagat gatattcaac catcatcaag aagatatcct ttcgttaaat tcacacctga
     2161 gcgcgaaatc ggtaatgact tactaatcgt tcaaaatcta tctaaaacga ttgacggtga
     2221 aaaagtatta gataatattt cattcacaat gaatccaaat gataaagcaa ttttaattgg
     2281 ggatagtgaa attgcgaaaa ccacattgct taaaatatta gccggtgaaa tggaaccaga
     2341 cgaaggttca tataaatggg gtgtaacaac gtcattaagt tacttcccta aagataactc
     2401 agaattcttt gagggcgtta atatgaacct tgtagattgg ttaagacaat acgcaccaga
     2461 agatgagcaa actgaaacat ttttacgcgg cttcttaggc cgtatgctat ttagtggaga
     2521 agaagttaag aaaaaagcta gtgtactttc aggtggagaa aaagtacgtt gtatgctaag
     2581 taaaatgatg ttatcaagtg caaacgttct tttacttgat gaaccaacaa accacttaga
     2641 cttagaaagt attactgctg ttaatgatgg acttaaatca ttcaaaggtt ctattatctt
     2701 tacttcatat gactttgaat ttatcaatac aatcgcaaac cgtgttatcg atttaaataa
     2761 acaaggtggc gtttcaaaag aaattccata tgaagaatat ttacaagaaa tcggcgtttt
     2821 aaaataaaca taatatgtag aatttctttt cgaaaatctc tgtattgagg cccgaccaac
     2881 ttgcacatta ttgtaagttg gcttactgtc accttctata ttgggactta ttccgcaact
     2941 tgtattgttt gtagaaatta ggaatccaat ttctctatgt gttgcaccga caaggattga
     3001 aaaaagctag ctacaaagtc attttcattc agtcaactac tgtcaatata acattgcagg
     3061 gcctagggca ttgattcatg tccgagacta tattcaattt aatagacatt aactgggcgt
     3121 ctcgatgcta atatttatag catcgaggtg tcctttttat tttatttttt aacacatcag
     3181 catagtgaac accccttctc taaattacaa atacagttat caacgcatta aaagagtaca
     3241 tttatttaat gtgcatattg attatgctat aagtattttg aattttattt ttcaaaaagt
     3301 ctcattatca ccacctttta aaatataagc aacgctataa cttgaatttt caggttattc
     3361 caaaaaatct aaattatcgg tttacattca tatttgtcat atgtataatg aacacatacc
     3421 aatttaataa tattttgatg aggcgcatca atcatgagta aagtttagat tactgtctgc
     3481 taacagctaa atttgaaagg gtgcgatgcc gaagcaatta taatagcagt tataatttgt
     3541 tggacttttt ggttaagagc tgagagtttg tcattattta aaaataatgg agtgcatcac
     3601 ttgtatatag attaagagca agttcgcatt ccgaacttgt tcttttttta ttattgtgtg
     3661 cccttcctaa caattaggag gattatatgg taacaagaag tgtgttgaaa tttggcggat
     3721 catccgtcag tgattttaca aaaataaaaa ggatcgctga aatgttaaag gagcgagtca
     3781 atcaagatga acaattaatt gtcgttgtaa gtgctatggg taacacaaca gatcaattaa
     3841 tgacgaatgt atcaaccttg actaaagcac caaaacaaca agaactggca ttattattga
     3901 caaccggaga gcaacaaact gtatcttatt tatcaatggt attaaatgat atcggtatga
     3961 atgccaaagc aatgactggc tatcaagcgg gtattaaaac cattggccat catttaaaaa
     4021 gtaaaattgc tcaaattaat cctcaaacat ttgaacaagc ctttcaagaa aacgatattt
     4081 tagtagttgc tggatttcaa ggcatcaatg aacatcagga attaacaact ttaggcagag
     4141 gtggttctga tacgaccgct gtggcacttg ctgttagtaa tcaaatacct tgtgaaattt
     4201 ataccgacgt tgatggtgtg tatgccactg acccaagact tttaccaaag gctaaacgac
     4261 tagacatcgt ctcatatgaa gaaatgatgg aaatgagcgc tttaggtgct ggtgtacttg
     4321 aaacaagaag tgttgaatta gctaaaaact ataatatccc tttatattta ggaaaaactt
     4381 tatcgaacgt gaaaggaaca tggattatat caaatgaaga aatattagag aaaaaagcag
     4441 ttactggtgt ggctttggat aaacatatga tgcatgtaac aattagttat cccctacctg
     4501 acaatcagct acttacccaa ctatttacgg aacttgaaga aggtgctgta aatgttgata
     4561 tgatttcaca aatcgtcaac ttggatgggc tacaactatc cttcacgatt aaagatagtg
     4621 attttcatca aatttctatg attcttgaaa cattaaagaa tcaatatgaa gcattagctt
     4681 ataaaatcaa tgagcattat gtcaaaattt cattaattgg ctcaggcatg cgtgatatgt
     4741 caggtgtggc atcaaaagca tttttgacat taattgaaaa taatatacct ttctaccaaa
     4801 caacaacatc tgaaataagt atttcatacg tcattgatga ttttaatggg caacaagcgg
     4861 tagaaaaact atatgacgca tttaacattt aatggtaaaa tgattgttaa aatattctaa
     4921 aaattggaaa ttattataaa atggagtgac aagttatgac aaagttagca gttgtgggtg
     4981 caacaggatt agtaggtaca aaaatgttgg agacattaaa tcgtaaaaat attcctttcg
     5041 atgaattagt attattttca tcagcacgtt ctgcagggca agaagttgaa tttcaaggaa
     5101 aaacatatac agttcaagaa ttaactgatg ctcgtgcaag tgaacatttc gattatgtat
     5161 taatgagtgc tggtggcggt acaagcgaac actttgcccc actttttgaa aaagctggtg
     5221 caatcgttat agacaattca agtcaatggc gtatggcaga agatattgat ttaatcgttc
     5281 cggaagtcaa tgaacctaca tttacaagag gtatcattgc caatccaaac tgctctacga
     5341 ttcaatctgt tgtacctcta aaagtattgc aagatgctta tggtttaaaa cgagtggcat
     5401 atacaacata tcaagctgta tcaggttcag ggatgaaagg taagaaagat ttagctgaag
     5461 gtgtaaatgg taaagcacca gaagcatatc cacatccaat ttataataat gtgttaccgc
     5521 atattgatgt gtttttagaa aacggatata caaaagaaga acaaaaaatg attgatgaga
     5581 cgagaaaaat tttaaatgcg ccagacttaa aagtaacagc aacatgcgca cgtgtgcctg
     5641 ttcaagatag tcatagtgtt gaaattgatg taacgcttga caaagaaaca acagcagaag
     5701 atattaaagc gttatttgat caagatgacc gcgttgtttt agtagacaat ccagagaaca
     5761 atgaatatcc aatggcaatc aattctacta ataaagatga agtgtttgtt ggccgtatac
     5821 gtagagatga ttcattagaa aatactttcc atgtatggtg tacatcagac aatttattaa
     5881 aaggtgctgc attaaatgct gtacaagtat tggaacaagt tatgcgttta aaaggagcga
     5941 attaaaatga cacatttatt tgagggtgtt ggcgttgcac ttacaacccc ttttacaaat
     6001 aacaaagtta atattgaagc tttgaaaaca cacgttaatt ttttactaga aaataatgcc
     6061 caagcaatca tcgttaatgg aactactgct gagagcccta ctttaacaac agatgaaaaa
     6121 gaacgcattc taaaaacagt tattgatctt gtagataaac gtgttcctgt catagcagga
     6181 actggcacta atgatactga aaagtcaatc caagcttcaa tccaagctaa agccttaggg
     6241 gctgatgcaa ttatgttaat tacgccctac tacaacaaaa cgaaccaacg tggtttagtc
     6301 aaacactttg aagcgattgc agatgctgtg aaattaccag tcgtgctgta caatgttcct
     6361 tcaagaacga acatgacaat tgaaccagaa actgtagaaa tattaagtca acatccttat
     6421 atagttgctt taaaagatgc tacgaatgat tttgagtatt tagaagaagt gaaaaagcgc
     6481 attgatacaa attcatttgc attatatagt ggcaatgatg acaacgtcgt cgaatactat
     6541 caacgtggcg gtcaaggggt tatctctgtt attgccaatg tcattcctaa agaatttcaa
     6601 gcgttatacg atgctcaaca aagtggatta gatattcaag atcaatttaa accaatcggc
     6661 acactgttat cagctttatc agttgatatt aacccaattc ctattaaagc tctaacaagt
     6721 tatttaggat ttggaaatta tgaattacgt ctaccattgg ttagcctaga agatacagat
     6781 actaaagtgc ttcgtgaaac atatgacaca tttaaagcgg gtgaaaatga gtgaaaatat
     6841 tactaattgg ctatggcgca atgaatcaac gcgttgctag attagcagaa gaaaaaggac
     6901 atgaaatcgt tggggtcatt gaaaatacac cgaaagcaac aacgccatat caacaatatc
     6961 aacatattgc agatgttaaa ggtgccgatg ttgcaataga tttttcaaat ccaaatctgc
     7021 ttttcccttt attagatgaa gattttcatt tgccattagt tgtggcaaca actggcgaga
     7081 aagaaaaact acttaataag ttagatgaat tgagtcaaaa tatgcctgtg tttttcagcg
     7141 cgaacatgag ttatggcgtt catgcattga ctaaaatttt agcagctgct gttcccctac
     7201 ttgatgattt cgacatcgaa ttgactgagg cacatcataa taaaaaagta gatgcaccaa
     7261 gtggtacgtt agaaaaattg tatgatgtga tcgtatcttt gaaagaaaat gtaacacctg
     7321 tgtatgatag acatgaatta aatgaaaaac gccagccaca agatattggt atacattcta
     7381 ttcgtggagg tacgattgtc ggtgaacatg aagttctatt tgctggcact gatgaaacga
     7441 ttcaaatcac gcatcgtgca caatcaaaag atatttttgc gaatggtgca atacaagcag
     7501 cagaacgctt agttaataaa ccaaacggct tttatacgtt tgacaactta taaacatatt
     7561 aaaggagatc gattatttta tggtacaaca tttaacagct gaagaaatta ttcaatatat
     7621 aagtgatgct aaaaagtcta caccaataaa agtatattta aatggtaatt ttgaaggcat
     7681 cacatatcca gaaagtttta aagtatttgg ttcagaacaa tctaaagtaa tcttttgtga
     7741 agcggatgat tggaaacctt tttacgaagc atatggtagt caattcgaag atatagaaat
     7801 tgaaatggat cgtcgcaatt ctgctattcc attaaaagac ttaacaaata cgaatgcacg
     7861 aattgaacca ggtgcgttta ttagagaaca agccattatt gaagatggtg ctgtcgttat
     7921 gatgggcgca acaattaata ttggcgcagt cgttggcgaa ggtacaatga ttgatatgaa
     7981 tgctactctc ggtggtcgtg ctacaactgg taaaaatgta catgtagggg ctggcgcagt
     8041 attagcaggt gtgattgaac cccctagtgc ttcaccggtt ataatcgagg atgatgtatt
     8101 aatcggtgca aatgcagtta ttttagaagg tgtacgtgtt ggtaaaggtg ctattgttgc
     8161 agctggcgcg attgtgacac aagatgtacc agctggtgca gttgttgctg gtacacctgc
     8221 aaaagtgatt aagcaagctt ctgaagtaca agatactaaa aaagagattg tagcagcatt
     8281 aagaaaactg aatgactagt gacgtcaacg tataataatt tcaaggttga gatacgatta
     8341 tgtctcaacc ttattgttta gcattgaata aacttatgat cacaaatttc gaaatacgtt
     8401 acactattta tatagtaatt taacacaaag gattgataat tatgaatgaa ttagaatttg
     8461 ttacgaaaca tcgccgtcat ttacatcaac atcctgaatt aagcttacat gaatttgaaa
     8521 caactgctta tattaaagcg tttttagata gtttaaatat taaatacgat tgcccattgg
     8581 aaactggcgt cattgcatac ttagaaggta atggctcaca tacgatagcg tatagagctg
     8641 atattgatgc gttacctatt ttagaggaaa atgatgtgcc ttatcgcagt caatctgatc
     8701 atgtgatgca tgcttgtgga catgatggtc atacaactgc attaatgctt tttgtacaac
     8761 gttgcaaaga catgcaagat gcaggtcaat taccgcaaaa tgtcgttttc attttccaac
     8821 ctgcagaaga aactggtggc ggtgcaaatc gattaataaa agccggtgcc tttgataagt
     8881 atccaattga agcggtattt ggtattcatg ttaacccatt tgctgatgaa ggcattgcag
     8941 tgataagaga tgaagaaatt acggccagcg caacagagta tcgctttttc ttaacaggcc
     9001 tgtcaagtca tgttgctgat aaagaacaag gtcattcttg tggtgaagca ttacaacatg
     9061 tattaactca aatatcacaa attcaacaat ttcaccttaa cggtttgaaa cgaaatattg
     9121 ttcatattgg tcattttaaa gctggtgaag cgattaacac tgtaccaagt aatggctatt
     9181 tagaaggtac tattcgtaca tatgatattg atgatttaac aatcgttaaa aatcaaatgc
     9241 acaagatagc agaaagtgtc aagcttctgt ttaatgtaga ttgtgaagtt aaatttgcag
     9301 aaggttatcc ccctacaatc aatagtccga aattacgtac tcaaatagag gacgccttaa
     9361 taaaagctga tttaaatgtc tatgacaaac caacgccatt cttatttggg gaagatttta
     9421 gtttttatgg tcaacaacta gctccagctt actttgtttt tataggaaca cgaaatgaag
     9481 ataaaggttt tgtaactggt ttgcacacat cacatttaaa ttttgatgaa aaagtgttaa
     9541 taaacgtggt taatttttac gaaaatttat taaataatta caaagaggtg taatacattg
     9601 acagcaacat ggtctgtaaa taagaaaata tttttacaaa atgcaatcac agtcaaaaac
     9661 aatcagccat taatggcagt tgttaaaaat aatgcatatc actatgacct agaatttgct
     9721 gtaactcagt ttatccatgc aggtatagat acatttagca caacatcact acgagaagca
     9781 attcaaatta gacaacttgc tccagatgca acaatctttt taatgaatgc agtttacgag
     9841 tttgatttag tccgtgaaca tcaaatacat atgactttgc cgtcgttgac atattactat
     9901 aaccataaaa atgatttagc tggtattcat gttcacttag aatttgaaaa tttattacat
     9961 cggtctggat ttaaagattt aaacgaaatt aaagaagtat tgaaagatca ccatcataat
    10021 caaaatgcaa aaatgattat tagtggttta tggacccatt ttggatatgc tgatgaattc
    10081 gatgtgtcag attataatgt tgaacgttca caatggatgg aaattgttga agcactttta
    10141 tctgaaggtt atcagttcga cctaatccat gctcaaaata gtgcgagttt ttatcgggaa
    10201 ggacaaatat tactacccca ccatacacat gcgcgtgtag gtattgcgtt atacggttca
    10261 agaccatata gttcactgaa tcaacatgat atagttcagt cattaacttt aaaagcacat
    10321 gttattcaag tgcgcgaagt acaagctggt gattattgcg gttatagctt tgcctttgaa
    10381 gtgactaaaa acaatacaaa attagctgta gttgatatcg gttatggcga tggaatttta
    10441 agaactcgtg ctaaacatga agcacttatc aatggtaaac gctacccgat acgtgcatta
    10501 atgatgagcc atatgtttgt tgaagtagat ggcaatgtac atgcacaaga tgaagttatt
    10561 ctttataata atgatatccg catcgatgaa tataccttta aaggtgttgg tgcaaattct
    10621 gaacaattaa gtgctatgaa tcatgattct ttaaaaaagg agtacatttc aaatgactgt
    10681 taaatataat caaaatggcg aattaacaat ggatggtatt agtttaaaaa cgattgcaca
    10741 aagctttggt acacctacca ttgtttatga tgaactacaa attagagaac agatgcgccg
    10801 ttaccatcgc gcatttaaag atagtggatt aaaatacaat atttcatacg cctcaaaggc
    10861 atttacttgc attcaaatgg tcaaacttgt agctgaggaa gatttacagt tagatgttgt
    10921 ttctgaaggt gaattatata cagctttaga agcaggtttt gaaccgagtc gcatccattt
    10981 ccatggtaac aataaaacga aacatgaaat taggtatgct ttagaaaata atatcggtta
    11041 ttttgttata gattcattag aagaaattga attaatagac cgctatgcta atgatacggt
    11101 tcaagttgta ttacgagtta atccaggtgt tgaagcacat acacacgaat ttattcaaac
    11161 tgggcaagaa gatagtaagt ttggattatc aattcaatat ggcttagcta aaaaagcaat
    11221 tgacaaagtc caacaatcta aacacttaaa attaaaaggt gtacattgtc atattggttc
    11281 acagattgaa ggtacagaag catttattga aactgctaaa attgttttac gttggcttaa
    11341 agagcaaggc attcaagttg aattattaaa ccttggtggt ggctttggta ttaaatatgt
    11401 tgaaggtgac gaaagtttcc ctatcgaaag tggtattaaa gatattacag acgcaataaa
    11461 atccgaaatt aaagttctag gtatagatgc accagaaata ggtattgaac cgggacgatc
    11521 aattgtaggt gaagctggcg ttactttata tgaagttgga accattaaag aaattccaga
    11581 gattaataaa tatgtttcaa tcgatggcgg tatgagtgat catatcagaa ctgcacttta
    11641 tgacgcaaag tatcaagcat tgcttgttaa tagaaatgaa gaagcagatg acagtgtaac
    11701 tatagctgga aaattatgtg agtctggtga tatcattatt aaagacgcta aattaccttc
    11761 atcagtcaaa cgtggagact atcttgctat attatcaact ggtgcatatc attactctat
    11821 ggcatccaat tacaatcaaa tgcaaaagcc ttctgtgttt ttcttaaaag atggcaaagc
    11881 acgtgaagtt ataaagcgac aatcgttaag acaactcatt attaatgata caaaataaaa
    11941 ataaacaaag taatccccga agcacagaaa attatactgt gactcgggga atttttatgc
    12001 attatagatg actgtagaaa acatgcttgg acatatactc taaaaaagga agaacagcac
    12061 atttttagtt agaaagtatt tatttctttt tgtcattaat cctttatatt tagttagccc
    12121 tattcaacta atttaaagca tcatatacat accacacttt aaacaagcat catgaaatta
    12181 gattttatgc tttacttgct ttttcaaata atataaaacg ataagaatta acaacaaata
    12241 taatgtataa aacagaattt catcaaatcc aaaatcttta cttgtaatta atacatca
//
LOCUS       DAKTIP010000065        12280 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAKTIP010000065 DAKTIP010000000
VERSION     DAKTIP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.065"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            8..1363
                     /locus_tag="O0305_001348"
     CDS             8..1363
                     /locus_tag="O0305_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCY0274497.1"
                     /translation="MWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSV
                     TDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAM
                     TALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGP
                     MARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQT
                     QDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAF
                     MIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATN
                     LIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYS
                     MVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVT
                     I"
     gene            1610..3460
                     /gene="nrdD"
                     /locus_tag="O0305_001349"
     CDS             1610..3460
                     /gene="nrdD"
                     /locus_tag="O0305_001349"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCY0274498.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            3457..3993
                     /gene="nrdG"
                     /locus_tag="O0305_001350"
     CDS             3457..3993
                     /gene="nrdG"
                     /locus_tag="O0305_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCY0274499.1"
                     /translation="MILLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            4093..4203
                     /locus_tag="O0305_001351"
     CDS             4093..4203
                     /locus_tag="O0305_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274500.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(4219..4380)
                     /locus_tag="O0305_001352"
     CDS             complement(4219..4380)
                     /locus_tag="O0305_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274501.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKITVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            4512..6134
                     /locus_tag="O0305_001353"
     CDS             4512..6134
                     /locus_tag="O0305_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0274502.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            6398..7084
                     /locus_tag="O0305_001354"
     CDS             6398..7084
                     /locus_tag="O0305_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0274503.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQDIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(7272..7835)
                     /locus_tag="O0305_001355"
     CDS             complement(7272..7835)
                     /locus_tag="O0305_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCY0274504.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINSLLESSDLTEAETQEAQKIKSQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(7924..8019)
                     /locus_tag="O0305_001356"
     CDS             complement(7924..8019)
                     /locus_tag="O0305_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274505.1"
                     /translation="MKNVSIYKTTQELSFVSSLDFYLTKHLTLML"
     gene            8088..9578
                     /gene="betB"
                     /locus_tag="O0305_001357"
     CDS             8088..9578
                     /gene="betB"
                     /locus_tag="O0305_001357"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCY0274506.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            9839..11548
                     /gene="betA"
                     /locus_tag="O0305_001358"
     CDS             9839..11548
                     /gene="betA"
                     /locus_tag="O0305_001358"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCY0274507.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            11826..12038
                     /locus_tag="O0305_001359"
     CDS             11826..12038
                     /locus_tag="O0305_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0274508.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
BASE COUNT         4157 a         1809 c         2398 g         3916 t
ORIGIN      
        1 tgataatatg tggttaacag taatggggct cattattatt atttcaattg taggtttact
       61 cattgccaaa aagataaatc cagttgtagg tatgacaatc ataccttgct taggggcaat
      121 gattttagga tatagtgtga cagatttggt tggatttttt gctaaagggt tagatcaagt
      181 catcaacgtt gttattatgt ttatctttgc cattattttc tttggcatca tgaacgatag
      241 tggtttattc aagccgcttg tcaaacgctt aatattaatg acacgaggca atgtcgtcat
      301 tgtctgtgca atgacagctt taattggcac aatagcccaa ttagatgggg ccggtgcggt
      361 aacatttttg ctttctattc ctgcattatt acctttatat aaagcgttaa atatgaataa
      421 atatttattg attttactat tagcattaag cgcggcgatt atgaacatgg taccttgggg
      481 aggtccaatg gctcgtgtag ctgcagtgtt aaaagccaaa agtgtcaatg aattatggta
      541 tggattaata cctattcaaa taataggttt cattcttgtt atgttgtttg cggtatatct
      601 tggatttaaa gaacagaaac gtatcaaaaa agcaatagag agaaatgaat taccgcaaac
      661 acaagatata gatgtacata aattagttga agtatatgaa cgagatcaag atgtaaggtt
      721 tcctgtaaaa ggacgtgcaa gaacaaaatc atggataaaa tgggtgaata cagctttaac
      781 tttagctgtt attctatcga tgttaataaa tattgcgcca cctgaatttg cattcatgat
      841 aggtgtttcg ttggcacttg ttattaattt taaatcagtg gatgaacaaa tggaacgatt
      901 aagagcgcat gcgccgaatg cattaatgat ggctgcagtg attattgcag caggtatgtt
      961 tttaggtgta ctaaatgaaa ccggtatgct taaagcgatt gcgaccaatt taatcaaagt
     1021 gattcctgca gaagtaggac catacttgca tattattgta ggtttacttg gcgtaccatt
     1081 agatttacta actagcacag acgcttatta ttttgctgtg ttaccgattg tcgaacaaac
     1141 agcagggcaa tttggtgtac cgtctgtatc aacagcttat tcaatggtca tagggaatat
     1201 tataggtaca tttgtcagcc cattttcacc agccttatgg ttggcaattg gtttagcaga
     1261 ggcaaacatg ggcacgtata ttaagtatgc attcttttgg atttggggat tcgctatcgt
     1321 tatgttagta attgcaatgt tgatgggcat tgtgacgatt taagtatgaa aaaatagaaa
     1381 ctatggtcac gttgcaaaat gaaataatag ttgcataaac atgtcgaaat gacggacgaa
     1441 tctttaaaca attttaaaaa ttaatgaaat aattgtgtag aaatatgaat ttcactaaat
     1501 gttaataact ttgtgacgtt ttagttaaca gactaataaa aatttgaaaa tactatatat
     1561 agtggtataa cgtaatgagt agacacaata tataggaaga aggggtaaaa tgaatcaaat
     1621 cgaagaagca ttaacgggtt tgatttctaa agatcctgct attgttaacg aaaatgctaa
     1681 caaagatagt gatacatttt caacaatgag agatttaaca gcaggtatcg tttctaaatc
     1741 ttacgcatta aatcatttat taccaaagca cgttgcagat gcacatcaaa gaggggacat
     1801 acattttcac gacttagatt atcatccatt ccaaccgtta actaactgtt gtttaataga
     1861 tgctaaaaat atgctacata atggatttga aataggcaac gcgaatgtaa cttcaccaaa
     1921 atcaatacaa actgcatcag cgcagcttgt acaaattata gccaatgttt ctagcagtca
     1981 atatggtggc tgtacggttg accgcgttga cgaattactt agtacatatg cacgacataa
     2041 tgaagaacaa cataggaata tcgcaaagca atttgtcaaa gaatctgaaa ttgatcgtta
     2101 tgttgatcaa caagtcacta aagacatcaa tgatgcgatt gaaagtttag aatatgaaat
     2161 taatacctta tatacatcta atggacagac accttttgta acattaggat tcggcttagg
     2221 tacagatcat ttaagtcgca aaattcaaca agctatctta aatactcgta tcaaaggctt
     2281 aggaaaagac cgcacgacag cgattttccc gaaacttgta ttttcaatta aaaaaggaac
     2341 caactttagt ccgcaagatc cgaactatga cattaaacaa ctagcattaa agtgttcaac
     2401 gaaacgtatg tatccagata ttttaaatta tgacaaactc gtagaaatat taggtgattt
     2461 caaagcgcca atgggttgtc gttcattttt accaagttgg aaagatgcgg aaggtcattt
     2521 tgaaaataat ggtcgttgta atcttggtgt tgttacactt aatttaccta gaatggcatt
     2581 agaatctgcc ggtaatatga cgaaattctg ggaaatcttt tatgaacgta tcgatgtgtt
     2641 acatgatgca ttactttatc gtataaatcg tttgaaagat gctgtaccga ataacgcacc
     2701 gattttatat aaaagtggcg catttaacta taaattaaaa gaaacagatg atgttgctga
     2761 gttatttaaa aataaacgtg caacgatttc aatgggctat atagggttgt atgaaacagc
     2821 tactgttttc tatggtccag actgggaaac atctcaagaa gcaaaagcat ttacgcttga
     2881 aattcttaaa gaaatgaaac gttatcaaac gaaatggaca gaattatatg acatttggtt
     2941 cagtatttac agtacgccga gtgaatcgct aacggatcgt ttttgtcgtt tagaccaaga
     3001 gagatttgga gatattaaag acattacaga taaaggatat tatcaaaact ctttccatta
     3061 tgatgtacgt aaagatgtta caccttttga aaagttagat tttgaaaaag attatcctta
     3121 ttatgcgagt ggtggtttca ttcactattg tgagtatccg aaattgcaac acaatttgaa
     3181 agcactagaa gcggtatggg actactctta tgacaaagtt ggttacttag gtacaaatat
     3241 tccgattgat cattgttatg aatgtgatta cgatggagat tttgaagcaa ctgaaaaagg
     3301 atttaaatgc ccgaactgtg gcaatgataa tcctaaaaca gttgatgtcg ttaaacgaac
     3361 atgtggttac ctaggcaatc cagttcaacg tccagtaatt aaaggccgtc ataaagaaat
     3421 ttgcgcacga gtaaaacata tgaaagcgcc taaagaatga tacttttaga cattaaacaa
     3481 ggacaaggtt atattgctaa aatagaatca aatagctttg ttgacggtga aggagtaaga
     3541 tgcagtgttt atgtatcagg atgtccattt aattgtgttg gatgttataa caaagcctca
     3601 caaaagttca gatatggcga gaaatacact gatgaaatat tagcagaaat attagatgat
     3661 tgcgatcatg attatatatc tgggctaagt ctattaggtg gcgaaccatt ttgtaatttg
     3721 gatattacat taaatcttgt caaagcattt cgagcacgtt ttggaaatac aaagacaatt
     3781 tgggtatgga ctggattttt atatgaatat ttagcaaatg attgtacaga acgtcgagag
     3841 ttattatcat acattgacgt tttagtagat ggtctattta tacaacactt attcaaacct
     3901 gatttaccat ataaaggttc tttaaatcaa cgcattatag atgtacaaca atcactctcg
     3961 catgcgcgta tgattgaata tatagttagt tgaatatgta ttagaagtca aggtaacatt
     4021 cgttgccttg gcttcttttt aggttaggta cataattgaa agttaataaa agcaattctt
     4081 tataaaaata tattgataga atatgaccta acaatcattt tgataccaat actaaaagtt
     4141 gcatatccgt tttttaaaaa agttgaaaga gaaaagtggt attttagtgg gaaggaagtc
     4201 taactttttg gtagcgtttt acaataaata aatattcgtt aataacgtat aaatattctt
     4261 aaatgccatt ctagtaaaat ttgttaaatt cgttaaatcg taacttaaca ctgttatttt
     4321 agcgctatta aggttttgtt tattacggga aaaattatat aaatattcaa taattgccaa
     4381 gtttcaaatt gtatgaaatt tgcattatta ttaaatgtta gttattgtca attttgtgaa
     4441 tcaatataat tattacattt tgagataaat cgaaacagga ttcataaaat taataattag
     4501 ggggagcaca attgaaaaaa gagaaagtta tggactggac gacctttata gggacagtag
     4561 ctgtacttct ttttgcagtt atacctatga tggcttttcc aaaagcaagt gaagatatca
     4621 tcactggtat taatagtgcc atttctgatt caattggttc gatatattta tttatggggc
     4681 tggcgatttt ttgctttgta atgtatatcg catttggtaa atatggtaat gtcacacttg
     4741 gtaaagcaag tgataaacca gaatttaata catttacatg ggcggcaatg ctgttttgtg
     4801 caggcatagg ctctgatatt ttatactggg gcgttattga atgggctttt tactatcaag
     4861 ttccaccaaa tggcgcgaaa agtatgagtg atgaagcact ccaatatgcg acgcaatatg
     4921 gtatgttcca ctgggggcca attgcttggg ctatttatgt tctaccagca ttaccaattg
     4981 gttatttagt atttgttaaa aaacaaccgg tgtataaaat tagtcaagct tgtcgtccga
     5041 ttttaaaagg tcaaacagat aaatttgtag gtaaagttgt agatatctta tttatctttg
     5101 gattgctagg tggtgcggca acatcactag cgttaggtgt gccattaatt tctgcaggca
     5161 tagaaagatt aactggttta gatggtaaaa atatgatttt acgttcggcc attttattaa
     5221 caatcacggt tatatttgcc attagttcat atacaggatt gaaaaaaggt attcaaaagt
     5281 taagtgatat caacgtttgg ctatcctttg tacttttagc ctttatattt attattggac
     5341 cgactgtttt tattatggaa acgacagtga cagggttcgg aaatatgttg agagatttct
     5401 ttcatatggc aacatggtta gaaccattcg gtggtattaa aggtcgaaaa gaaacgaatt
     5461 tcccacaaga ctggacaata ttctactggt catggtggtt agtatatgcg ccatttatcg
     5521 gtttatttat cgctagaatt tcaaaaggtc gacgccttaa agaagtcgtg ctaggaacaa
     5581 ttatttatgg aacgcttgga tgcgtattat tctttggtat ttttggtaac tatgctgtgt
     5641 atttacaaat ttctggacag tttaatgtaa cacaatattt aaatacacat ggtacagagg
     5701 caaccattat tgaagtggtg catcatttac cattcccatc attgatgatt gtactattct
     5761 tagtatctgc tttcttattc ttagcaacaa catttgattc gggttcatat attttagcgg
     5821 cagcatctca gaaaaaagtg gtaggcgaac cattacgtgc caatcgttta ttctgggcat
     5881 ttgcattgtg cttattgcca ttttcattga tgctagttgg tggtgaacgt gcattagaag
     5941 tattgaaaac tgcttcaata ctggcaagtg tgccattaat tgttattttt attttcatga
     6001 tgatatcatt tttaatcatt ttagggcgcg atagaattaa acttgaaacg cgtgctgaaa
     6061 aattaaaaga agttgaacgt cgttcattgc gaatcgttca agtatcagaa gaagaacaag
     6121 acgataattt ataattcaaa gcgggtctgg gacgacgaaa tgaattttgt gaaaatatca
     6181 tttctgttcc attccccttt ttttagtagc attgtaggat gaacttttag gttttcatta
     6241 atgttgtact aaaagattta attttttagt gctccaagta cttatttatt gtatgaagca
     6301 tattctaaat cgaagtttga aagactctca ttgattatta aattaaataa agggtactgc
     6361 gtatgtacaa ttcaaattaa tcgaaggatg aaataaaatg actaatcaat ttaaaaataa
     6421 acagtccaaa ttacatgaca gtttagaatc catcacaaaa aacttatatg cgacacctac
     6481 ttcggaactg ccttttgata atcgatttct atttaaatct tttattttaa aacgtgaaac
     6541 aggtaatatt gttatctatc attccggaca cttaggtgac tcccaacaag acattgcatc
     6601 attaggtggt gtttcaaaag tattgatgaa tcatgatcat gaatctatag gaggttctaa
     6661 tcaagttgaa gccccttact ttatacatga aaatgatgtg gctgcactga aacataagat
     6721 ttctgttcaa aaacaattta gtaatcgtgt aatgttggat aaggatttag aagttattcc
     6781 cgcgcctgga catacaccag ggacgacact atttttatgg gatgatggtc atcaccgtta
     6841 cttatttact ggagatttta tatgttttga agggaagaga tggcgtacag ttatattagg
     6901 ttcaagtgat agagaaaaat ctattcaaag tttagagatg gttaaagaat tagattttga
     6961 tgtacttgta ccttgggtta ctatcaaaga tgaaccgtta gtttattttg tagaaaatga
     7021 atatgaaaaa cgtgaacaaa tacaaaatat tattgataga gtacgtgagg gcgagaatag
     7081 ctaattgaaa tatattggcg aagcaatgta acgaatctaa gaaagcccta gaaaatacct
     7141 ccataattga ttgtcatata aaacaaaaac ggtaatttct atttattgag atagaaatta
     7201 ccgtttattt cgtggaccta ttgcattgtt tttatcatgc ataatcatca ttgtcgttgt
     7261 ttgagtcaat tttaattttc agaatcagaa ggctgttctg gaattgggaa atatttgaaa
     7321 atttcaccgc tttcaatcgc ttcggttaac tgttctaacc attcgtaata aacatgtgta
     7381 tgatcaagct gagatttaat tttttgtgcc tcttgtgttt cagcttcagt taaatcactg
     7441 ctttcaagta atgaattgat aatagcttga gcatctttta ctgcttcgac attgatgtca
     7501 atttcacgct ggaatttttt agtgaaaaag tttcggaaaa agatgaaaaa gtctttctcg
     7561 gcgataaaat gttgtttgcg gcttcctctc gtaaattgtt gtttaacaat atcaaattcc
     7621 tgcaatttct taacgccagc actcatactt ggtttgctca tttgcaattg atgacgcatt
     7681 tcatcaagcg tcatactgcc ttcaaacacc attgtgccat ataagtttcc tacacttcta
     7741 ttagtgccat acaaatccat tgtctgtcca attgaattaa ttacaatatc ttttgcttgt
     7801 tctaattgtt gctgtttgtt ctgagaacga gtcatcattg cacctccgta catcattttg
     7861 gtcacgttaa aataaatact aatacattat aaaacctttt ctaaaaaaag acattaaaaa
     7921 tatttaaagc attaaagtta aatgtttcgt taaataaaaa tctaacgaac ttacaaaact
     7981 taattcttga gttgttttgt aaattgacac atttttcatt tctatgctaa cataagtttg
     8041 taaaattcgt taaataaaaa tttaacaaac ttaacggagg ttgttgaatg gaacttttaa
     8101 aacatttatc tcagcgtcaa tatattgatg gtgagtgggt tgaaagcgcg aataaaaata
     8161 caagagatat tatcaatcct tacaatcaag aagtgatatt tacggtttct gaagggacaa
     8221 aagaggatgc agaacgtgca atcttagctg caagacgtgc gtttgagtct ggtgaatggt
     8281 cgcaagaaac tgctgaaaca agaggtaaaa aagtacgtgc catcgcggac aagattaaag
     8341 aacatcgcga agcgttagca cgattagaaa cattagatac tggaaaaacg ttagaagaat
     8401 catatgcaga tatggatgat attcataatg tgtttatgta ttttgctgga ttagcagata
     8461 aagacggtgg cgaaatgatt gattcaccaa ttccagatac agaaagcaaa attgttaaag
     8521 aaccagtagg tgtagttaca caaattacac cttggaatta tccgttatta caagcatcat
     8581 ggaaaattgc gccagcgctt gctacgggtt gttcactagt tatgaaacca agtgaaatta
     8641 caccattaac aacaatacgt gtttttgaat taatggaaga agttggtttc cctaaaggaa
     8701 caattaatct tattctaggt gcaggttctg aagttggtga cgtaatgtca ggtcataaag
     8761 aggttgacct tgtatcattt acaggtggca ttgagactgg taagcatatt atgaaaaatg
     8821 ctgctaataa tgttacgaat attgccttgg aacttggcgg taaaaatcca aacattatct
     8881 ttgatgatgc tgattttgaa ttggcagtag accaagcgtt aaatggtgga tatttccatg
     8941 caggtcaagt ttgttcagca ggatcaagaa tattagtaca aaacagtatt aaagacaaat
     9001 ttgagcaagc acttattgat cgcgtgaaaa aaatcaaatt aggtaatggt tttgatgctg
     9061 atactgaaat gggaccagtg atttcaacag aacatcgtaa taagatcgaa tcttatatgg
     9121 atgtagctaa agcagaaggc gcaacaattg ctgttggtgg taaacgtcca gatagagatg
     9181 atttaaaaga tggtctattc ttcgagccaa cagtcattac aaattgtgat acgtcaatgc
     9241 gtattgtaca agaagaggtt ttcggacctg tcgttactgt agaaggcttt gaaactgaac
     9301 aagaagcgat tcaattagcg aatgattcta tatatggttt agcaggtgct gtattttcta
     9361 aagatattgg aaaagcacaa cgcgttgcta acaagttgaa acttggaacg gtgtggatta
     9421 atgatttcca tccatatttt gcacaagcgc catggggtgg atacaaacaa tcaggtatcg
     9481 gtagagaatt aggcaaagaa ggcttagaag agtaccttgt ttcaaaacac attttaacaa
     9541 atacaaatcc acaattagtg aattggttta gcaaataaaa attagataag gtgagtgcca
     9601 ttgtaagaac acaagacact cactttgttt tgtataagtg gcgaaatgtt gattgataat
     9661 ttggactaaa cgcaaaatga atcatagatt atttcattac tgttagtaac aatcgtaaaa
     9721 ggaaaagcga gtgttttggt tagctaagtt tagcaattca acgataacca atcagccact
     9781 aacaaatatt tcatgcaata ctcactttga aatacaacaa actttggagg tcataacgat
     9841 gagtaacaaa aacaaatcat atgattatgt catcattgga ggaggcagtg caggttctgt
     9901 actaggtaat cgtctgagtg aagataaaga taaagaagtc ttagtattag aagcgggtcg
     9961 cagtgattat ttttgggatt tatttatcca aatgcctgct gcgttaatgt tcccttcagg
    10021 caataaattt tacgattgga tttattcaac agatgaagaa ccacatatgg gcggtcgtaa
    10081 agtagcacat gcccgaggta aagttttagg tggatcaagt tcgattaatg gcatgattta
    10141 tcaacgtggt aatccaatgg actatgaagg ctgggcagaa ccagaaggta tggaaacttg
    10201 ggattttgcg cactgtttac cgtattttaa aaaattagaa aaaacatacg gtgcagcgcc
    10261 ttatgataaa tttagaggcc atgatggacc aattaagtta aaacgagggc cagcaacgaa
    10321 tcctttattc cagtcattct ttgatgcagg tgttgaagca ggctatcata aaacacctga
    10381 tgtgaatgga tttagacaag aaggttttgg accgttcgat agtcaagtac atcgtggtcg
    10441 ccgaatgtca gcttcaagag catatttaca tccagcgatg aagcgtaaaa acttaaccgt
    10501 tgaaacacgt gcctttgtaa ctgaaattca ttatgaaggt agaagagcaa ctggtgttac
    10561 gtataagaaa aatggcaaac tacataccat cgatgctaat gaagtcattt tgtctggtgg
    10621 ggcattcaat acgccacaat tactacaatt atctggtatc ggtgattcag agttcctaaa
    10681 atcaaaaggc attgagccac gtgttcattt acctggtgtg ggtgaaaact ttgaagatca
    10741 cttagaggta tacattcaac ataaatgtaa ggaacctgta tcattacagc caagcttaga
    10801 tatcaagcgc atgccattta ttggtttaca atggattttc acacgtacag gtgcagcagc
    10861 atctaaccat tttgaaggtg gcggatttgt acgttcaaat aacgaagttg attatcctaa
    10921 cttaatgttc catttcttac caatagctgt aagatatgat ggtcaaaagg cagcggtcgc
    10981 acatggttat caagttcacg taggaccaat gtattcaaac tctcgtggta gcttgaaaat
    11041 caaatctaaa gatccattcg aaaaaccaag tatccgcttt aattatttat caacagaaga
    11101 agataaaaaa gaatgggtag aagcaattcg tgtagcaaga aatatcttat ctcaaaaagc
    11161 aatggatcca ttcaatggtg gagaaatttc accaggacca gaagtgcaaa cagacgaaga
    11221 aattcttgat tgggttcgta gagatggtga aacagcatta cacccatctt gtagtgctaa
    11281 aatgggtcct gcttcagatc caatggcagt agttgacccg ttaacaatga aagtccacgg
    11341 tatggaaaat ttacgtgtag ttgatgcatc agctatgcca cgtacgacaa atggcaatat
    11401 acacgcacct gttttaatgt tagctgaaaa agcagcagac attatacgtg gcagaaagcc
    11461 tttagagcca caatatattg attattataa gcatggtgtt catgatgaaa atgaaggtgc
    11521 aatcgaagta aaaccatacg ctaaataaat ttatatagta gactcttagt ctggcatcta
    11581 ttgtattggt gccaggcttt tttgcgtttt atctaaatga gaagttaatg tgttgttcaa
    11641 aaacggtgtg tgagtatatg aactgaaaat agagttaatt gtacattgtg atgattaatt
    11701 gacctaattg gcagacatta aaagagtgca caaccttaaa aagttcacta aatagacatt
    11761 taattgtgtt ttcttgaaag ctttcgtggt aggatttata taagtaaatt gagagaagga
    11821 atactatgag cttttacgaa tatatacaaa catttaaaga tgataaaaca ccattaggcg
    11881 aattagcgat ttggattaaa gaagatgatt cattcccaaa acaagagaag ctaacagaaa
    11941 acattttgtc ttattttcat caaatgtcca atatagatca tgagttttta gaaattgtaa
    12001 aaagatcact ttctctgtat gatcaattaa aatcgtaaaa tcagaaataa agttagctaa
    12061 aataaaaggc cggtgatttc acagatgaag ttgtcggcta ttttcatgac taatgttaaa
    12121 taatgatcat gaaattgaag acacaaaaat agtgggcgac tcctgtcccc catttgcatg
    12181 aaatattaat actttttaca aagtcttatc ttttaaaaag aagccaaact atgattgtaa
    12241 ttaataaata cgggaatgaa ttagtaatag atataaagta
//
LOCUS       DAKTIP010000066        12229 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAKTIP010000066 DAKTIP010000000
VERSION     DAKTIP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.066"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(5..217)
                     /locus_tag="O0305_001360"
     CDS             complement(5..217)
                     /locus_tag="O0305_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274509.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQEQI"
     gene            complement(247..858)
                     /locus_tag="O0305_001361"
     CDS             complement(247..858)
                     /locus_tag="O0305_001361"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCY0274510.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(858..1550)
                     /gene="pyrF"
                     /locus_tag="O0305_001362"
     CDS             complement(858..1550)
                     /gene="pyrF"
                     /locus_tag="O0305_001362"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCY0274511.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(1660..4833)
                     /gene="carB"
                     /locus_tag="O0305_001363"
     CDS             complement(1660..4833)
                     /gene="carB"
                     /locus_tag="O0305_001363"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCY0274512.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(4826..5926)
                     /gene="carA"
                     /locus_tag="O0305_001364"
     CDS             complement(4826..5926)
                     /gene="carA"
                     /locus_tag="O0305_001364"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCY0274513.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(5928..7202)
                     /locus_tag="O0305_001365"
     CDS             complement(5928..7202)
                     /locus_tag="O0305_001365"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCY0274514.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(7220..8101)
                     /locus_tag="O0305_001366"
     CDS             complement(7220..8101)
                     /locus_tag="O0305_001366"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCY0274515.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(8129..9436)
                     /locus_tag="O0305_001367"
     CDS             complement(8129..9436)
                     /locus_tag="O0305_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCY0274516.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(9654..10181)
                     /gene="pyrR"
                     /locus_tag="O0305_001368"
     CDS             complement(9654..10181)
                     /gene="pyrR"
                     /locus_tag="O0305_001368"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCY0274517.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(10580..11497)
                     /locus_tag="O0305_001369"
     CDS             complement(10580..11497)
                     /locus_tag="O0305_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0274518.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTSEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(11497..11988)
                     /gene="lspA"
                     /locus_tag="O0305_001370"
     CDS             complement(11497..11988)
                     /gene="lspA"
                     /locus_tag="O0305_001370"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCY0274519.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
BASE COUNT         3736 a         2361 c         1774 g         4358 t
ORIGIN      
        1 gtttttaaat ttgttcttgt ttacgtaata tatcctttac atgtttaaat aaatcatcat
       61 tatgatctat agttgtttta ttttttatat gtttttgctt cgttgaactt tcgtcgacat
      121 cgtgactact agatagattg gaatcatttg aatgattgat aaattcgtta caatgtttta
      181 tcttcccata taaatcatta gatgttttgt cattcatttg ttgaattcct ccttctagtg
      241 tctatattat gctaagttgt ctctccattc aactaatgtt tggatatctt cactagaaat
      301 tttaccttca ttttcagcta cttcaattaa ttcattgtaa tcacttaaag tgtaaaaagg
      361 tagttgaata ttgctaaatg tatcatctgc ttttttcaaa ccgtaagtaa agatagctac
      421 aacacctaat acttctgcac ctgctagttt taaggcttca actgctgtga ctgaagatcc
      481 ccctgtcgaa attaaatctt ctatcacaac tactttttta ccttcacttt tagcaccttc
      541 gatttgattt tgcttaccat gactcttatt tgatgaacga acataattca ttggtaattt
      601 taatttttca gcaataaaag ctgcatgtgg aataccagct gttgcagtac cagaaataac
      661 ttctacttca ggaaagtgtt ctttaattaa gttaattaaa ccatcgcgga ttgcgcctcg
      721 aactaaagga taacctaacg taacacggtt atcacagtaa atcggtgatt taatacctga
      781 actccatgta tataaatcat ttggtgataa tgttacagct tcaatatcta ataatgattt
      841 tgcaatttct ttagccatta tactaaccaa ctttctttaa ttttatgata actttcgact
      901 ggattgtcac tttgtgtaat cggtctaccg actacaatat gcgtcgaacc aagctgtctt
      961 gcttcttccg gtgtcgtaat acggtgttgg tcattttgag atgcaccttt aggtctaata
     1021 cctggtgtta cttttaaaaa tgatgtacct aacttttcag tcaacatacg actttcaaga
     1081 ggtgaacaaa caacgccatc taaaccagct gcatttgcta acttggcata atttaaaacg
     1141 gcctcttcaa tcgatgtttg tatattttgt tcgtgtcgta acatgtcttc tgttgttgac
     1201 gtaagctgtg ttactgcaat aatttttgta tcttgattat gttttcttaa tcctttaatg
     1261 gcctcagaca tcatttttac gccaccagca gcatgaacat ttaccagatc aacattcaat
     1321 ttagctagtc cttccatcgc cttaccaact gtattaggaa tatcatgcag ttttaaatct
     1381 aaaaatacat catggcctct ttcttttatc tcattaatta attgaggacc ttcttgataa
     1441 aaaagttcca tacctacttt tacgaataat gattcatcaa ataaatctaa aaattgattt
     1501 actttttctt ttgattcaaa atctaatgca ataattggta aatctttcat catatatgtc
     1561 tccctttata ttaagaaatt cattaaaata atgaggctgt aatataaact tctttaagta
     1621 aatggttaat tacagcccca ccgatacagt ttgattgatt tacatttgac gcattgtaaa
     1681 tgtcatactt tcaattacat tcgttaaggc attagctgta tctaaagatg tcaaacatgg
     1741 aataccattt tcaactgtag tacgtctaat ttggaagcca tccctttcta cttctttacc
     1801 tttagtcatt gtatttataa cgatttgaac atcaccattt tgaatacgtg ttaataaatc
     1861 attttcgcca ccaattttgc ctactacttc tgcaggtatg tcatactcag ctaatttatt
     1921 agctgttcca gacgttgcta aaattttata gccaacttca tttaagcgtt gtgccaattt
     1981 aacaacttcc tctttatctt tgtcactgac ggtcattaat actgtaccgt gatctttaac
     2041 ttcaacgcca ctacctgtta accctttgaa taacgccttt tctaatgtag tatctttccc
     2101 catcacttca cctgttgact tcatttcagg tcctaaagta atatcaacat ttttcaattt
     2161 attaaaacta aatactggtg ctttcacaaa gacaccctca gcatatggtt gaaccccttc
     2221 ttgataaccc atatctgtta gtttttcccc aatgattgct cgcatagcta attgtgccat
     2281 tggaatatca gtaattttac ttaagaatgg taccgttcta ctagaacgtg gatttacttc
     2341 taaaacatac acaccatcgt gagctataac gaattgaatg ttgattaagc caatgatgtt
     2401 taaaccttta gctaatttta tagtatagtc ctcaagtgtt gctaactcgt cttctgtcaa
     2461 agtttgtggt ggatatacag cgattgagtc accactatgc acaccagcac gttcaatatg
     2521 ttccatgatt cctggaataa tgaccgtttc tccatcacaa atcgcatcaa cttcaatttc
     2581 tttaccagtt aaatatctat cgactagtac cggatgttcc ggactcgctt ttacagcctg
     2641 ggtcatatag ttttctaact ctttgtcatt gtctacaatt tccattgcgc gaccacctaa
     2701 tacataagaa ggtcttacta caaccggata tccgatttct gcagcattcg ctaatgcttc
     2761 ctcaggtgat gtagctgttt tcccttgtgg ctgtggcacg ttaatttttc ttaatagtgc
     2821 ttcaaattct tttctatctt cagcacgatt tagattttct agtgaagtac caagtatttt
     2881 aacaccatgt ttagccaatt tgtctgctaa attaatcgct gtttgtcctc caaattgtac
     2941 aacgacacct ttaggttttt ctaaattaat gatattcatc acatcttctt cagttaaagg
     3001 ttcaaagtat aatttgtcag aaattgagaa gtctgttgaa actgtttctg gattgttatt
     3061 cacaattatc gcttcgtacc ctgctttttg aattgcccaa acggcgtgaa ctgtcgcata
     3121 gtcaaattct acaccttggc cgattcgaat tggtccagag cctaatacta agattttttc
     3181 tttgtcagta actatggatt cattttcagt ttcgtatgta ccataataat atggtgttga
     3241 agattcaaat tcagctgcgc aagtatcaac catcttgtaa acaggtttaa tatcattttc
     3301 catacgcaat tgatatactt cttcttccgt catattaaag cgatgcgcta ttgttttatc
     3361 actaaatcca taatcttttg catatttaag atattctaaa tcaccttgat gctcttttaa
     3421 ttgatgctca atatcaataa tgttttggaa cttgtgtaag aagaagtaat caatctgagt
     3481 catattatga atttcttcta atgttgtgcc tcttctaatt gcttcgccga tgaaaaataa
     3541 tcgttcatca tcttggtgtg aaatacgttc tttaatataa tcaagatcga agctttcacc
     3601 atttggtaat cctaagtgat gcacaccata ctcaagtgat cgaattgctt ttaacaatga
     3661 ttcttcgtaa gttcgaccaa tggccataac ttcacctgtt gctttcattt gtgtgccaag
     3721 ctctcgttct cctttttcaa atttatcaaa aggaaatctt ggtatttttg aaatcacata
     3781 gtctaaagtt ggttcaaacg ctgcataaga tgttcctgta attggattta acatttcatc
     3841 taatgttaga ccaaccgcga ttttagcagc taattttgca ataggatatc ctgttgcttt
     3901 tgaagctaac gctgatgaac gtgatacacg cggatttact tctataatat aataatcgaa
     3961 tgaatgggga tctaatgcta attgaacatt acaaccacct tcgataccta aagctcgaat
     4021 aacttttaat gaaacatcac gtaacatttg atactcaaca tctgataatg tttgactagg
     4081 agccacaaca attgaatcgc ctgtatgaat accaactgga tcaatatttt ccatgttaca
     4141 tacaacgatg gcattatcgt ttttatcacg cattacttcg tattcgattt ctttaaaacc
     4201 tgcgatagat ttttctaata aacattgcgt tgctggacta taatgaagac catttgagac
     4261 gatttcatgt aattcttcat cattatgaca aataccgcct ccggtaccac ccatcgtaaa
     4321 tgccggtcta acaattagcg ggtatcccac ttgctctttg aatttaaagg cttgctctac
     4381 tgtatttaca atgtcactct ctggtacagg aacgtttaaa tcattcatta atgttctaaa
     4441 catttcacgg tcttctgctt gttgaattga tgttagctca gttcctaata attggacgtt
     4501 attatcttga agcacaccac tttcgtgtag ttgaatcgcc atgtttaaac ctgtttgacc
     4561 acctaaagtt ggaagtaaag cgtcaggttg ctctttacgt ataattcgcg ctataaaatc
     4621 atgagttaac ggttcgatat atactttatc cgcaatttcc ttatcagtca tgattgtcgc
     4681 tggatttgaa tttacaagaa taactcgata tccctcttct tttaaagcta gacatgcttg
     4741 tgttccagca taatcaaatt cagctgcttg acctatgata attggcccag accctattac
     4801 taaaattgtt ttgatatcat tacgtttagg cattgatatg acgctccttt tccttaaaat
     4861 tagtcatcat tgctacaaaa tcatcaaata gatagtttga atctgacggt cctggatttg
     4921 cttcaggatg gtattgaaca gaaaatgctg gtaatgtttt atgttttaaa ccttctacag
     4981 taccatcatt taatgcaaga tgagtaactt ctaaatcagt actttttaac gaatctatat
     5041 ctattgcata tccatggttt tgactcgtaa tatcaacttt tccagtctct aaatttttaa
     5101 ctggatggtt cgcaccacga tgaccaaact tcattttaaa tgagcttgct ccttgagata
     5161 atgcaaaaag ttgatgtcct agacagatac caaagaacgg aattttccct aaaattcctt
     5221 gaatcattgg aatcgcacat tctacaactt caggattacc tggtccgttt gatagcataa
     5281 cgccatctgg agccattgct aaaatttctt cggcagtagt tgtatatggt acaactgtga
     5341 cgttacaacc tctgacgttt aattctcgaa caatattttg cttcttacca aagtctacaa
     5401 gtacgacact tagatcctta cctgtcgaaa catacggtgt tttagtcgaa acgatttcta
     5461 cttcgttttt aggtaattct acttgttgta aatgtttgac aagttgatca atatcttctt
     5521 ttcgatcagt aaaaccagct tttaacacac cgtgttgtcg aattttacgc gtaatacttc
     5581 ttgtatcaac acctgcaatc cctggaattt gatgcaattc taaaacgtca tgaagtgtct
     5641 tttgctgtct aaaattactt ggatgcgcac tcgcttcttt cactacaata ccgtttaatg
     5701 taggtactaa tgattcaaaa tcgtctctat tgataccata attaccgatt aatggatacg
     5761 taaaagtaat gatctgacct gtatatgatg gatctgaaat agtttcttga taacctgtca
     5821 tcgctgtatt aaatacaatt tctcctacag ttaagttatc agatcctaaa cggtagcctt
     5881 cgtaaaaaga accgtcttct aacactagat aacgtttgct ttgcatatta tttatccccc
     5941 tcaaatttaa cttcgccttc aaccattgtt aagatcggat ttccataaac tttatagccg
     6001 ataaatggtg tattatctgc ttttgataag aaatcttctc ctttaatttc ttgttcacta
     6061 tctaaatcaa tgattgttaa atctgcataa ccattttctt ttaatgtgcc gtattctaaa
     6121 ttaaatgtct cacatggttt aattgttaag taatctacta attgttgtaa tgtccaatca
     6181 ccatttttta caaaatgcgt atataataat gggaatgctg tttcactacc aacaattccg
     6241 aatggtgctt tttccattgg ttgtgctttt tcatcacgtg catgtggtgc atggtctgtt
     6301 gcgatacagt caattgtacc gtctagtaac ccttctaaca aagcctctct atcttcagta
     6361 cttctcaatg gtggattcat tttataaatg gcattattac caggaatatc atcttctgtt
     6421 aacaataaat ggtgtggtgt aacttcagct gtaacatgaa tgcctgcgcg tttagcgtca
     6481 cgaatgactc taacactttc tttagtagaa acatgacata catgataatg acaacctgct
     6541 gcttcagcta atagtacatc tcttgcgatt tgaacagatt cacaaatgtt tggaatacct
     6601 ggtataccta actctttact gcgtttccct tcatgcattg caccaccata gattaatgaa
     6661 ttatcttcac agtgtgctac gatggctttg tttacttttg cagcttcaat catgccttca
     6721 tacatcatgc ttgcagtttg tactcctaca ccgtcatctg taaacgcaaa ggcaccttct
     6781 tttactagtg ctgggaaatc aaccaattct ttacctaatt gacgtgttgt aattgaagca
     6841 taaggtaata cacgtacttg agcattgtca tcgattaatt tttgtaaagc ttcaaaatgt
     6901 tctacagaat ctggtaccgg tcttgtgtta ggcattggac atacagttgt aaatccgcct
     6961 ctagcagcag ctttagtacc agtttcaatt gtctctttat attcaccacc aggttcacgt
     7021 aaatgaacat gaacatcgac aaatccaggt gacacaaagt gacctttcgc atctatgatg
     7081 tcaacaccat tgcttggttc aattgcaggt gcaatttgtt taattacctt accatcaatt
     7141 aaaatatctg cttgttgtaa ttcgccattt tgtaatactt taccgttttt aattaatttc
     7201 atcattttcg tccccttacc tattttaata attcatcaat gactgccatt cttaagtaaa
     7261 cgccattttc catttgctta aaaattcttg attttgaagc ttctactaaa tcgctttgta
     7321 tttctactcc tctattcaca ggtgccggat gcataacaat agcatgttct tgtaatttgt
     7381 tatagcgtac ttcatttaag ccatgctttt gatgataatc atctgctgca aatctagttt
     7441 cttctgcaag cccatgcctt tcatgttgaa ttcttaataa cataactata tctactgttt
     7501 ctataacatc atctatattt acataaggtg cttctaaaga atcatcaatc caagcatttg
     7561 ggctattaaa cattacgttt gcacctaatg cttttaaact atggtaatta ctacgtgcga
     7621 cacgtgaatt tttaatgtct ccacaaatca atacattcaa gccttcaaaa tatccatatt
     7681 cttcatatat cgtcattaaa tcaagtaaac tttgtgttgg atgttgtcca ctaccatcac
     7741 cagcattcgc aattgggatg ttaatattcg ctaatttttc atagtagtta ttaaacggat
     7801 gtctaatgac taataaatca cagccaatac tttctaaagt tttacatgtg tcatataaag
     7861 attcaccttt tgaaacagat gatgttgatg tttcaaagct aatcgttttt aaccctagct
     7921 taagttctgc catttcaaaa ctacattttg ttcgagtaga attttcaaag aataaatttg
     7981 cgacatattt cccttcaaag tttggtagtt gacgttcacc agatttaaat tgacttgcct
     8041 tttggataag tttgtatatt tgatctgtag ataaatgttc cattgataat aaatgattca
     8101 taaaagcgcc ctccttaatt tgtaaatctt aattgttttg ttttttctct ttaggtaaga
     8161 ttaagttcaa aataattcct gaaagtgcag ctaatgccat cccctcaatt tgaaggttga
     8221 taccaatttc ttttaagtta aatactaaat taccgatacc tacaactaaa attacagaag
     8281 ctataactaa atttcgattg ttcgcaaaat ctactttgct ttcaactaac attcttaagc
     8341 cacttgctgc aataataccg aaaagtaata tagatactcc tcccataact ggtgtaggta
     8401 tagaagaaat taatgcagtg aacttaccaa tgaatgctaa aacaattgct ataactgctg
     8461 caccaccaat gacgtaaata ctgtatattc tggtaatcgc taatacaccg atattttcac
     8521 cgtatgttgt acttggtggt ccaccaataa tactggcaaa cattgtagaa acaccatcac
     8581 caatgattga tttatcaagt cctggctttt caaagaagtt tctacctacg attttattca
     8641 ataccatttg gtgcccaata tgttcactta ctgttacgaa cacaatcgga atcattacaa
     8701 gtacaagtcc taagtgaaat gaaggtacat aatctttaaa tggcagataa atatgaggga
     8761 aatctatcca ttttgctgac ataattgcat caaatttcac aatccccata aaaatcgcta
     8821 ccacataacc tacgataata cctacaagaa ctggaattaa agataggaat cctttaaaaa
     8881 atccttgaac gactattgtt acaagcaagg ttatcattgc aacaattaag aaactgatat
     8941 tgtaaccttt catatctcca ggattttcat acattgccat attgactgca gtaggcgcta
     9001 agcttaaacc aattaccatg atgactggtc caacaacaac tggtggtaat aatttcatta
     9061 accatgctgt cccacttaat ttgattagaa tcccgatgat gacgtacata acaccactca
     9121 tgaataatgc tacaagcatg tctcctaagc tatgcgtact taatcccgtg ataattggcg
     9181 tgataaatgc aaagctagat cccaagtatg ctggtatttg cgccttcgtt attaagatat
     9241 aaagtaatgt accgattccc gaagctagta acgctgctga tattggtagt cctgttaaga
     9301 atggtactag tactgttgcg ccaaacatcg caaataaatg ttgtaagctt aaaaatgccc
     9361 attgcgctgg ttgtggtttt tcatttacat ctagtacggg ttttactgtt cgttcaaaca
     9421 tttcatcatt ttgcataata ttcatttcct ccgataataa aaaaatctct ttacatcagt
     9481 atatgtaaag agacaaaaag tgtgacaagt tgctacaagt cattttcgtc catagaaatt
     9541 gacttatagt tgtcgaacat gagggtatta ttagataaac aagcatatga aaacttattt
     9601 atcattcaac tcccccacct ttttcagtct ctcgtactga attaaaaggg gtattattta
     9661 attataactg catttctttg atccatttct tctaaataga cacttaccgt ttcctcttta
     9721 gaagtaggta tatttttacc aacaaaatct gctcgaattg gtaactcacg atgtcctcga
     9781 tcaaccaaag cagctaaacc aattttaata ggtctagcat ttagcaaaat agcatcaagt
     9841 gaagcacgaa ccgttcgacc agtatacagc acatcgtcaa taatgatgac tactttatct
     9901 gtaatatctg tgtcgatgtc tattgcgtct tttgtcgtaa gtgatgacat gtgctctata
     9961 tcatctctaa agtatgtaat atcaattgtt ccagtaggta tacgttgttg ctcaatttga
    10021 tgaattttat cttgtatacg attcgctaaa tattcacctc ttgttttgat acctaaaaga
    10081 attaaattat cagtaccttt gttatactcc aaaatttcgt gagcgattct cgtcactgta
    10141 cgttgtattg cggcatcatc cattatgata cgttcagaca tattctcacc ctttcaaaat
    10201 ttagaaacaa aaaacctcat gtctttaata tcgacatgag gtttagtata tacaatcagc
    10261 ctatttacat agaagaatcg tagtattact acttattctt gcatatgtga aagtgttatg
    10321 atcattttag tatatatcta tattttcatg tctttgacgt ctctctggac gctcaattaa
    10381 agactcttta tgttgtactt aaactgttat aaggataata ctaatcaggc gctttgtcaa
    10441 tctatattga aaaattaacc atactcagta aaaatgttaa ccttaatcta ttttataatg
    10501 tgttttcatt taaaaaaacg aaaacgataa acacttatag tttacataat aatgttatcg
    10561 taattatagc acgacacaat tatgcatctc tttttcgaat tgtatctaat aaatcttcaa
    10621 agtcttgtgg taattcagca tgtctttcaa tatattcact tgttactgga tgttcgaatc
    10681 caataagtcc agcatgtaga gcttgaccac caatatccaa tgtctttttc ggtccatact
    10741 ttggatcacc aactaatggg aagccaatat atttcatgtg aacacggatt tgatgcgtac
    10801 gtcctgtttc aagttgacat tcaacaagcg tataatcttt aaaatgttct agtacgttaa
    10861 aatgtgtcac tgcttcctta ccatcatcaa caacagccat agattgacga tcatttttgt
    10921 ttctaccaat tggcgcatcg attgtaccgt aatcatgagg aatattcccg tgaactaaag
    10981 cgatatattt tcttttaaca gatttatcca ttaattgttc tacaagccca cgatgagcaa
    11041 tatcattttt agcaaccatt aataaaccag aagtatccat atctatacgg tgaacaatac
    11101 ctggacgaat ttctccatta atacctgaca aatttttaat ttgatacatt aaaccattaa
    11161 ctaatgtatt ggtataatgc cctggtgatg gatgaactac catgcctttc ggtttatata
    11221 caactgcaac atcgtcatct tcataataaa tatctaaatt taaattttca ggtagaatat
    11281 cagcttcaac cacttctttt tcagtgacaa ctatatgatc attaagtttc actttataat
    11341 tagatttaac aactttatcg tttgcaacga ctaaacctgc tttaatccaa tcttgtatct
    11401 ggttacgaga ccaatcatta tttaattcag gcagcaactt atctacacgc atacctgttt
    11461 gttctttatc tgtaatgtta aattcataag tctccattac ttaacctcct tctccttttt
    11521 attggaagta tcctttaata aggcaataat aattaatatt acaccaattg ttaaacttga
    11581 atctgcgata ttaaatattg gaaaatcata accaaaaata tttgtatcaa taaagtcaac
    11641 aacttctcct gttaaaattc tatcaataaa gtttccaagt gcacctgcaa aaagtaaact
    11701 aatagcaact tgcataaaca aattatattg agcatcttta ataaagaaat atactaaggc
    11761 tattaatata ataatggtaa taataaagaa aaatgtcatt tttccactca atattcccca
    11821 tgcagcacca ttatttcgat gtgatgttat gtttaaaaag tgcggtatca cttcaaatga
    11881 atctccaatt ttcattgtag tagctataat atatttagta acttggtcaa atataacgac
    11941 aaatactgct attaaaatgg aagtgccaat aaaatatttt ttgtgcattt tcgttcctcc
    12001 aatcaatcgt ccatgagaca actctttata ttatagctta cacctgctaa taaaaaaagt
    12061 aagcatatta cattaaatct aatgttacta actcaatact tgataaacta ctatgttttg
    12121 acattaaata tgaacttaat tattcattta tcatatttaa gatgacatta aaaattagga
    12181 aagcaggctg gaacataaat ccctaaaaag acagtagtaa gatattttc
//
LOCUS       DAKTIP010000067        12142 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAKTIP010000067 DAKTIP010000000
VERSION     DAKTIP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.067"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            175..1410
                     /gene="arcA"
                     /locus_tag="O0305_001371"
     CDS             175..1410
                     /gene="arcA"
                     /locus_tag="O0305_001371"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0274520.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1443..2453
                     /gene="argF"
                     /locus_tag="O0305_001372"
     CDS             1443..2453
                     /gene="argF"
                     /locus_tag="O0305_001372"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0274521.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            2544..3974
                     /gene="arcD"
                     /locus_tag="O0305_001373"
     CDS             2544..3974
                     /gene="arcD"
                     /locus_tag="O0305_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0274522.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            3991..4932
                     /gene="arcC"
                     /locus_tag="O0305_001374"
     CDS             3991..4932
                     /gene="arcC"
                     /locus_tag="O0305_001374"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0274523.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            5031..5735
                     /locus_tag="O0305_001375"
     CDS             5031..5735
                     /locus_tag="O0305_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0274524.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            6085..8784
                     /gene="clfB"
                     /locus_tag="O0305_001376"
     CDS             6085..8784
                     /gene="clfB"
                     /locus_tag="O0305_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCY0274525.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNSQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASNFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDISKLSDSYYADP
                     NDSNLKEVTDQFKNRIYYEHPNVASIKFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     VDPVTNRDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPEPSP
                     DPDPDSDSDSDSGSDSDSGSDSDSESDSDSDSDSDSDSDSDSESDSDSESDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSESDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKVSKQHK
                     TDALPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
     gene            8900..9661
                     /locus_tag="O0305_001377"
     CDS             8900..9661
                     /locus_tag="O0305_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCY0274526.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNSDTTVKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRINVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            9906..10361
                     /locus_tag="O0305_001378"
     CDS             9906..10361
                     /locus_tag="O0305_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0274527.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(10464..10574)
                     /locus_tag="O0305_001379"
     CDS             complement(10464..10574)
                     /locus_tag="O0305_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274528.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(10644..12068)
                     /locus_tag="O0305_001380"
     CDS             complement(10644..12068)
                     /locus_tag="O0305_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCY0274529.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
BASE COUNT         4247 a         1877 c         2294 g         3724 t
ORIGIN      
        1 aatgtatatt ttgacccaaa atgcctttat ttatgtataa aaatccatta ttatgtattg
       61 tataacaaaa agatatgaaa ttttcgactt tctttatgtg aatataatca catgtaagcg
      121 tttgaagatt gtctatactc taaatgaatt caaagataaa aggaggaaat agacatgaca
      181 gatggtccaa ttaaagtaaa tagcgaaatt ggagctttaa aaactgtgtt acttaagcgt
      241 cctggaaaag aattagaaaa tttagtacct gattatttag atggattact atttgatgat
      301 attccatatt tagaagtagc tcaaaaagag catgaccatt ttgcgcaggt gctaagagaa
      361 gagggtgttg aagtacttta ccttgagaag ttagcagctg aaagtattga aaatcctcaa
      421 gtaagaagtg aatttattga tgatgtatta gcagagtcta aaaaaacaat attaggtcat
      481 gaagaagaaa ttaaggcatt atttgcgaca ctttctaatc aagaacttgt agataaaata
      541 atgtcagggg tacgtaagga agaaattaat ccgaaatgta cacatctagt agagtatatg
      601 gatgataagt atccattcta tttagatcca atgccaaacc tttattttac tagagatcca
      661 caagcctcaa taggacacgg tataacaatc aatcggatgt tctggagagc acgacgacga
      721 gaatcaatat ttattcaata tattgtaaag catcatccta gatttaaaga tgcgaatatt
      781 ccaatctggt tagatcgaga ttgcccattc aatattgaag gcggcgatga acttgtttta
      841 tctaaagatg tcttggctat aggcgtttca gaacgtacat ctgcacaagc tattgaaaag
      901 ttagcgcgac gtatttttga aaatccgcag gcgacgttta aaaaagtagt agcaattgaa
      961 attccaacta gtcgaacttt tatgcactta gatacagtat ttacaatgat agattatgac
     1021 aaatttacaa tgcattcagc cattttaaag gcagaaggca atatgaatat atttattatt
     1081 gaatatgatg acgtaaataa agatattgcc atcaaacaat ctagtcattt aaaagatact
     1141 ttagaagacg tactaggtat agatgatatc caattcattc caacaggaaa tggtgatgtc
     1201 attgatggtg ctagagagca atggaatgat ggctcaaata cattatgtat aagaccaggc
     1261 gttgtagtga cttacgatag aaactatgta tcgaatgatt tattgagaca aaaaggcatt
     1321 aaagtcattg aaatatctgg tagcgagttg gtacgtggac gtgggggccc tagatgtatg
     1381 agtcaaccat tattcagaga agacatttaa cgtaataaat tatagaaacg agggtgaaaa
     1441 taatgacaga aattcaaaaa ccgtatgatt taaaaggcag atcattatta aaagaaagtg
     1501 attttaccaa agcagaattc gaaggactta ttgattttgc aattacatta aaagagtata
     1561 agaaaaacgg tattaagcat cactacttat ctggaaaaaa tattgcacta ctattcgaaa
     1621 agaattcgac gagaacgcgt gctgcgttta cagttgcgtc tattgattta ggtgcgcatc
     1681 cagaattttt aggaaaaaat gatattcaat taggcaaaaa agaatctgta gaggatactg
     1741 cgaaagtatt aggtagaatg ttcgatggta ttgaattccg tggtttttca caacaagctg
     1801 ttgaagattt agcgaagttc tctggtgtac cggtgtggaa tggattaaca gacgattggc
     1861 atcctacaca aatgttagct gattttatga caataaaaga gaattttgga tatctagaag
     1921 gaataaactt aacttacgtt ggagatggac gtaataatat tgcgcattca ttaatggtag
     1981 caggtgctat gttaggtgtt aatgtaagaa tttgtacacc taaatcatta aatccaaaag
     2041 aggcatatgt tgatattgca aaagaaaaag cgagtcaata tggtggttca gtcatgatta
     2101 cggataatat tgcagaagca gttgaaaata cagatgctat atatacagat gtttgggtat
     2161 cgatgggtga agaaagtgaa tttgaacaac gtattaattt attaaaagac tatcaagtga
     2221 atcaacagat gtttgattta acaggtaaag attcaacgat attcttacat tgtttaccag
     2281 cattccatga tacaaataca ctttatggac aagaaattta tgaaaaatat ggattagctg
     2341 aaatggaagt tacagaccaa atctttagaa gtgaacattc aaaagtgttt gatcaagctg
     2401 aaaatagaat gcatacaatt aaggcagtaa tggcagcaac attggggagt taatcactaa
     2461 atggaacgat atgaatatga tgtgtctgat gatataagtg tcatgtacag acacctcata
     2521 ttggtattaa aggagaaatg aatatgaacg aatcaggaga taacaaactc agtaaatctt
     2581 ctttaattgg actagttata ggatccatga ttggtggcgg tgcgttcaat ataatgtctg
     2641 atatgggcgg taaagccggt ggattagcca ttattattgg ttggattatt acagctatag
     2701 gaatgatttc attagcgttc gtatttcaaa atttaaccaa tgaacggccg gagctagacg
     2761 gtggtattta tagttatgct caagcaggat ttggcgattt tgtaggattt atcagtgctt
     2821 ggggatattg gttctcagcg tttttaggca atgttgccta tgcaacacta ttgatgtcag
     2881 cagtaggtaa ctttttcccg atttttaaag gaggcaacac attaccaagt gttattgtcg
     2941 cctcgttact actctggggt gtccatttct tgattttaaa aggcgttgaa acagcagcat
     3001 ttatcaatag tattgttact gttgcaaagt taataccgat tttacttgta atcatatgca
     3061 tgataattgc attcaatttt gacactttta aaacaggctt tttcagtatg acgtcagagg
     3121 gtgtattgcc atttagttgg gcgagcacaa tgagccaagt taaaagtacg atgctagtga
     3181 cagtttgggt gtttatcggt atcgaaggtg cagtaatttt ttctagtaga gctaaaaata
     3241 aaaaagatgt aggtagtgcc acggttatag gacttatatc agttttaatt atctatttct
     3301 tattaactgt attagctcaa ggcgtgattt tgcaaaatca tatttcgcaa ttagattcgc
     3361 caagtatggc acaggtgctt gcaactattg taggtggttg gggatctaca cttgtaaata
     3421 ttggtttaat tatttcggta ctaggtgcat ggttaggatg gacactgctt gctggtgaat
     3481 tacctttcat tgttgcaaaa gatggattat ttccaaaatg gtttgctaaa gaaaataaaa
     3541 atggagcacc tgtaaatgca ctgcttatta ccaatatatt agtacaatta tttttaataa
     3601 gtatgctatt tacacagagt gcgtatcaat ttgcattttc actagcatca agtgctattt
     3661 tataccctta catgttcagt gcattttacc aagttaaata cactttagag catcgacagc
     3721 aagcaactac taaacaatgg acgattggta tcatagcctc aatttatgct atatggctta
     3781 tatatgcagc aggtatcaat tacttattat tgacgatgtt actttatatt ccagctcttc
     3841 ttgtttatac aatcgttcaa aagaataatc agacacgttt gattaaatca gactatattc
     3901 tttttatgat tattatcgta cttgcagtta tcgggttaat taagttattg atgggaacga
     3961 taaatgtttt ttaaaaggag cgacaaaaat atgaaagaga aaattgtcat tgcattaggc
     4021 ggtaatgcga tacagacaac agaagcaaca gctgaagcac aacaaacagc tattagatgt
     4081 gcgatgcaaa accttaaacc tttatttgat tcaccagcgc gtattgtcat ttcacatggt
     4141 aatggtccac aaattggaag tttattaatc caacaagcta aatcgaacag tgacacaacg
     4201 ccggcaatgc cattggatac ttgtggtgca atgtcacagg gtatgatagg ctattggttg
     4261 gaaactgaaa tcaatcgcat tttaactgaa atgaatagtg atagaactgt aggcacaatc
     4321 gttacacgtg tggaagtaga taaagatgat ccacgatttg ataacccaac taaaccaatt
     4381 ggtccttttt atacgaaaga agaagttgaa gaattacaaa aagaacagcc agactcagtc
     4441 tttaaagaag atgcaggacg tggttataga aaagtagttg cgtcaccact acctcaatct
     4501 atactagaac accagttaat tcgaacttta gcagacggta aaaatattgt cattgcatgc
     4561 ggtggtggcg gtattccagt tataaaaaaa gaaaatacct atgaaggtgt tgaagcggtt
     4621 atagataaag attttgctag tgagaaatta gcaacgctga ttgaagcaga taccttaatg
     4681 attcttacga atgtagaaaa tgtatttatt aactttaatg aacctaatca acaacaaatc
     4741 gatgatattg atgtagcaac actgaaaaaa tacgcggcac aaggtaagtt tgtggaagga
     4801 tcgatgttgc caaaaataga agctgcgata cgatttgttg aaagtgggga aaacaaaaaa
     4861 gttatcatta ccaatttaga gcaggcatac gaagctttga ttggtaataa aggtacacac
     4921 attcacatgt agcactttat cacgcgacaa aacattaaat atgtttctcc gttgattcaa
     4981 atgaaaaagt tgtctgctga cactttgcaa ggtttgaagg agtttaactt atgacagaaa
     5041 actttatttt gggtagaaat aataaattag aacatgaact aaaggcatta gcagattaca
     5101 ttaatatacc atatagtata ttacaaccat atcaaagtga atgttttgtc agacattata
     5161 cgaaaggcca agttatttat ttttcgccac aagaaagtag caatatttac tttttaattg
     5221 aaggtaacat tattagagaa cattacaatc aaaatggaga tgtatatcgt tattttaata
     5281 aagagcaagt attatttcca atcagtaact tatttcatcc gaaagaggtt aacgaattgt
     5341 gtacagcatt aaccgattgt acagttcttg gattgcctag agaattgatg gcctttttgt
     5401 gcaaagctaa tgatgatata tttttgacac tttttgcatt aataaatgat aatgagcagc
     5461 aacacatgaa ctataacatg gcattaacaa gtaaatttgc taaagatcga attatcaaat
     5521 tgatatgcca tctatgtcag acagtaggat acgatcaaga tgaattttat gaaatcaaac
     5581 agtttttaac tattcaactc atgagtgata tggctgggat ttcccgggaa acagctggtc
     5641 atattattca tgaacttaaa gatgaaaaac ttgttgttaa agatcataaa aattggttag
     5701 taagcaaaca tttattcaat gatgtatgtg tttaatatac aatgtaaaat gaataagttg
     5761 aacatgaggt ctaacgtaca tttatacgtt aggccttttt tgctagcatg atgaataatt
     5821 taaaatgtta gttaaatttg attgttgaaa ttacagtaaa atttaaggtg atgaaaaatt
     5881 tagaacttct aagtttttga aaagtaaaaa atttgtaata gtgtaaaaat agtatattga
     5941 tttttgctag ttaacagaaa attttaagtt atataaatag gaagaaaaca aattttacgt
     6001 aatttttttc gaaaagcaat tgatataatt cttatttcat tatacaattt agactaatct
     6061 agaaattgaa atggagtaat atttttgaaa aaaagaattg attatttgtc gaataagcag
     6121 aataagtatt cgattagacg ttttacagta ggtaccacat cagtaatagt aggggcaact
     6181 atactatttg ggataggcaa tcatcaagca caagcttcag aacaatcgaa cgatacaacg
     6241 caatcttcga aaaataatgc aagtgcagat tccgaaaaaa acaatatgat agaaacacct
     6301 caattaaata caacggctaa tgatacatct gatattagtg caaacacaaa cagtgcgaat
     6361 gtagatagca caacaaaacc aatgtctaca caaacgagca ataccactac aacagagcca
     6421 gcttcaacaa atgaaacacc tcaaccgacg gcaattaaaa atcaagcaac tgctgcaaaa
     6481 atgcaagatc aaactgttcc tcaagaagca aattctcaag tagataataa aacaacgaat
     6541 gatgctaata gcatagcaac aaacagtgag cttaaaaatt ctcaaacatt agatttacca
     6601 caatcatcac cacaaacgat ttccaatgcg caaggaacta gtaaaccaag tgttagaacg
     6661 agagctgtac gtagtttagc tgttgctgaa ccggtagtaa atgctgctga tgctaaaggt
     6721 acaaatgtaa atgataaagt tacggcaagt aatttcaagt tagaaaagac tacatttgac
     6781 cctaatcaaa gtggtaacac atttatggcg gcaaatttta cagtgacaga taaagtgaaa
     6841 tcaggggatt attttacagc gaagttacca gatagtttaa ctggtaatgg agacgtggat
     6901 tattctaatt caaataatac gatgccaatt gcagacatta aaagtacgaa tggcgatgtt
     6961 gtagctaaag caacatatga tatcttgact aagacgtata catttgtctt tacagattat
     7021 gtaaataata aagaaaatat taacggacaa ttttcattac ctttatttac agaccgagca
     7081 aaggcaccta aatcaggaac atatgatgcg aatattaata ttgcggatga aatgtttaat
     7141 aataaaatta cttataacta tagttcgcca attgcaggaa ttgataaacc aaatggcgcg
     7201 aacatttctt ctcaaattat tggtgtagat acagcttcag gtcaaaacac atacaagcaa
     7261 acagtatttg ttaaccctaa gcaacgagtt ttaggtaata cgtgggtgta tattaaaggc
     7321 taccaagata aaatcgaaga aagtagcggt aaagtaagtg ctacagatac aaaactgaga
     7381 atttttgaag tgaatgatat atctaaatta tcagatagct actatgcaga tccaaatgac
     7441 tctaacctta aagaagtaac agaccaattt aaaaatagaa tctattatga gcatccaaat
     7501 gtagctagta ttaaatttgg tgatattact aaaacatatg tagtattagt agaagggcat
     7561 tacgacaata caggtaagaa cttaaaaact caggttattc aagaaaatgt tgatcctgta
     7621 acaaatagag actacagtat tttcggttgg aataatgaga atgttgtacg ttatggtggt
     7681 ggaagtgctg atggtgattc agcagtaaat ccgaaagacc caactccagg gccgccggtt
     7741 gacccagaac caagtccaga cccagaaccg gaaccaagcc cagacccgga tccggattcg
     7801 gattcagaca gtgactcagg ctcagacagc gactcaggtt cagatagcga ctcagaatca
     7861 gatagcgatt cggattcaga cagtgattca gattcagaca gcgactcaga atcagatagc
     7921 gattcagaat cagatagcga ctcagattca gatagcgatt cagattcaga tagcgattca
     7981 gattcagata gcgattcgga ttcagacagt gattcagatt cagacagcga ctcagaatca
     8041 gatagcgact cagaatcaga tagtgagtca gattcagaca gtgactcgga ctcagacagt
     8101 gattcagact cagatagcga ttcagactca gatagcgatt cagactcaga cagcgattca
     8161 gattcagaca gcgactcaga ttcagacagc gactcagact cagacagcga ctcagattca
     8221 gatagcgatt cagactcaga cagcgactca gactcagaca gcgactcaga ctcagatagc
     8281 gactcagatt cagatagcga ttcagactca gacagcgact cagattcaga tagcgattcg
     8341 gactcagaca gcgattcaga ttcagacagc gactcagact cggatagcga ttcagattca
     8401 gatagcgatt cggattcaga cagtgattca gattcagaca gcgactcaga ctcggatagc
     8461 gactcagact cagacagcga ttcagactca gatagcgact cagactcgga tagcgactcg
     8521 gattcagata gcgactcaga ctcagatagt gactccgatt caagagttac accaccaaat
     8581 aatgaacaga aagcaccatc aaatcctaaa ggtgaagtaa accattctaa taaggtatca
     8641 aaacaacaca aaactgatgc tttaccagaa acaggagata agagcgaaaa cacaaatgca
     8701 actttatttg gtgcaatgat ggcattatta ggatcattac tattgtttag aaaacgcaag
     8761 caagatcata aagaaaaagc gtaaatactt ttttaggccg aatacatttg tattcggttt
     8821 ttttgttgaa aatgatttta aagtgaattg attaagcgta aaatgttgat aaagtagaat
     8881 tagaaagggg tcatgacgta tggcttatat ttcattaaac tatcattcac caacaattgg
     8941 tatgcatcaa aatttgacag tcattttacc ggaagatcaa agcttcttta atagcgatac
     9001 aactgttaaa ccattaaaaa ctttaatgtt gttacatgga ttatcaagtg atgaaacgac
     9061 atatatgaga tatacaagca tagaaaggta tgcgaatgaa cacaaattag ctgtgattat
     9121 gcccaatgtg gatcatagcg catatgctaa catggcatat ggtcatagct attatgatta
     9181 tattttggaa gtgtatgatt atgttcatca aatatttcca ctttccaaaa agcgtgatga
     9241 caattttata gcaggtcact ctatgggagg atatggcaca attaaatttg cattaacaca
     9301 aggggataaa tttgccaaag ctgtaccatt atctgctgtg tttgaagcgc aaaatttaat
     9361 ggatctagag tggaatgatt tttcaaaaga ggccataatt ggcaatcttt caagtgttaa
     9421 aggaactgaa catgatccgt attacttgct agacaaagct gtagctgaag ataaacaaat
     9481 tccaaaattg ctcattatgt gtggtaaaca agacttttta tatcaagaca acttagattt
     9541 tatcgattat ttatcacgca taaatgttcc ttatcaattt gaagatggac caggagatca
     9601 tgattatgca tattgggatc aagcgattaa gcgtgctata acatggatgg tgaatgatta
     9661 attatttctt ggaaaatatg tggctgcatt aaatacacag agtgagagat acaaactatt
     9721 tacgcacgac taacatttct aagtgtttaa attatttttg tattaatatg attggcgcaa
     9781 tttgctgata cacaaaaatg tttctcgtga aacttagatt tagcttatag ttttatcatc
     9841 atttgtatga cttacattat aaattttatt ataatgaggt taacgctttg aaaggagtca
     9901 tcatcatgtc gaccaataaa aacgattatg agcatatgtt gttttatttt gcatataaaa
     9961 cctttattac taccgctgat gaaattatag agaagtatgg tatgagtcgt cagcatcatc
    10021 gttttttgtt ttttatcaat aaattacctg gtattactat taaatcatta ctagaaatat
    10081 tagaaatttc taaacaagga tcacatgcaa cacttcaaaa attaaaagag caaggtctca
    10141 ttattgaaaa agttttagag actgatcgac gtgtcaaaaa attatattcg acggataaag
    10201 gcgatcaact cattgctgaa ttgaacaagg cgcaagatga attattgcaa aatatatatc
    10261 aacaagtcgg ttcggattgg tatgatgtga tggaagcatt ggctaaaggg cgacctggct
    10321 ttgattttat taagcatttg aaagatgaaa aagaaagcta gcatcagaaa tgttaaaaat
    10381 cttcgcattc ttaaatttaa aaaatatgtc aaaaagtgta taataaaaac atataattta
    10441 attgaactca gtttcaacac atcttagaaa ggagtttgaa tgatgaaaaa attagcagtt
    10501 attttaacat tagttggcgg tttatacttc gcatttaaaa aataccaaga acgtgttaac
    10561 caagcaccta acattgagta ctaaattaaa ccataaaaaa ttcccgaaca ccttgttata
    10621 gtgctcggga atttttttat gctttacttg aatatatcaa atattatttt tgcgctttct
    10681 gtattttcga tattaccact aaatgattct gatctaggtc cgtaagcgta ggtattaaca
    10741 tcctcgcctg tatgtccatc ggaagtccac cctgtataag atttatcatt tactggcttc
    10801 tgaatagcgt gttgtagggc ttttgtttgc gtttctactt ctgcggattt ttcgtctttt
    10861 tcttttttaa gtagtctttt tagcttttta ttctcttttt taaccttttt catatcatct
    10921 tgtgaaaatt caaatccata accttcatta ataacttttt cagggtcttc acctttagcc
    10981 attttttctg tcatatatga tccagagtgt ttcatagatt taatcggttg aggattccat
    11041 tcgtatcctt tatctttacc aattgttaga ccaccagtag agtgatctgc tgttgcaacg
    11101 acaagtgtat ctttatgttt cttagcatat tgaatggcgt catcgaatgc tttttcaaaa
    11161 ccttccattt cagacataac gcctgtaata tcgttggaat gcgctgattt atctatagaa
    11221 gcaccttcga ccattaaaaa gaatcctttt ttattgcgct caagcttact aagtgcactt
    11281 tgttgcatat cagctaatga tggttcgtct ttagaagcat ctattgcaag tggcatattt
    11341 ttatctgcaa acaaaccaag aactttatct ttatcagatt ttgataactc cttactgttc
    11401 gtggcaaggt cgtaaccatc ttttttgaat tttttatcta aattgccatt acttttaccg
    11461 aaatatttag cgccgccgcc taataaaaca tcaactttat gctttccgtt gattttatct
    11521 ttataaaatt gtttagcgat ttcgttttta tcatctctag aagtcacgtg tgcagcatat
    11581 gctgctggtg ttgcatctgt taattcagct gttgaaacaa gaccagtcga cttacctttt
    11641 tcttttgcac gttcaagcac cgtctttact ttctgcttgt tactgtcaac accgatggca
    11701 ccattatatg tcttatgacc agaactaaag gctgttccgc cagctgcaga atcagtaata
    11761 ttctgttttg ggtcatttga atatgtacga tttgtgcctt ttaaatatga atcaaaagca
    11821 gtaggggtca tttctttagc atgcggatca tttttataat aacgataagc tgtgttaaat
    11881 gatggaccca tgccatcgcc aactaaaaag ataacatttt ttggattttt agtattacca
    11941 accgcgaaac tttcatcttt agaactttta tcggattgcg caattgcagg tgtgacagaa
    12001 ctaaaaaccg ttgacacgat aataaggtta gcaactgcaa attttgtggc ttttttaact
    12061 gataacataa gacatcctcc tgagtatatg actatgtctt cagtgtaaaa gaggaatttt
    12121 gagcaattat gtagttttag tt
//
LOCUS       DAKTIP010000068        12000 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAKTIP010000068 DAKTIP010000000
VERSION     DAKTIP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 12000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.068"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(92..757)
                     /locus_tag="O0305_001381"
     CDS             complement(92..757)
                     /locus_tag="O0305_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274530.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(757..1812)
                     /locus_tag="O0305_001382"
     CDS             complement(757..1812)
                     /locus_tag="O0305_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274531.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            1948..2622
                     /gene="hssR"
                     /locus_tag="O0305_001383"
     CDS             1948..2622
                     /gene="hssR"
                     /locus_tag="O0305_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCY0274532.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            2615..3988
                     /gene="hssS"
                     /locus_tag="O0305_001384"
     CDS             2615..3988
                     /gene="hssS"
                     /locus_tag="O0305_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCY0274533.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTIDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVNQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSL"
     gene            4151..4594
                     /locus_tag="O0305_001385"
     CDS             4151..4594
                     /locus_tag="O0305_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0274534.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDNVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            4591..5046
                     /locus_tag="O0305_001386"
     CDS             4591..5046
                     /locus_tag="O0305_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCY0274535.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YTHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVVYVLIWIIQFFKNKNYVDTINKQLKQLK"
     gene            complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="O0305_001387"
     CDS             complement(5112..6590)
                     /gene="mqo"
                     /locus_tag="O0305_001387"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCY0274536.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGV
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(6971..8569)
                     /locus_tag="O0305_001388"
     CDS             complement(6971..8569)
                     /locus_tag="O0305_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0274537.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTFTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            8886..10559
                     /locus_tag="O0305_001389"
     CDS             8886..10559
                     /locus_tag="O0305_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0274538.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKSNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAILPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(10712..11341)
                     /locus_tag="O0305_001390"
     CDS             complement(10712..11341)
                     /locus_tag="O0305_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274539.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKNQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(11655..>12000)
                     /locus_tag="O0305_001391"
     CDS             complement(11655..>12000)
                     /locus_tag="O0305_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0274540.1"
                     /translation="DSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKD
                     FQGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGD
                     VTDTDLIMEKEL"
BASE COUNT         4039 a         2132 c         1691 g         4138 t
ORIGIN      
        1 cctgtttttt gtaaacccaa atcgtattct gttatctaac ttttatacta ctatgtctta
       61 aatcattaac atttagcgtc tttacaagcc actaatcagt tattttgcca tcttctaatt
      121 caatcactcg atctgcatat tcaaataatc ttcgatcgtg tgtaatcata ataccaatca
      181 tttgttgttc tttaatttgt tgacgtatca tctcaacaac ttttgttgct ctatcggcat
      241 ctaaacttgc tgtgggctca tctgctaaaa tgattttcgg attattcata aatgctctca
      301 taatcgcaac acgttgcttt tcaccgccag ataactgatg cggatataca ttcaagcgat
      361 cttctaaacc aatatttttc aaaagttgta ttgctcttgt tgaactttgt tgtttggtca
      421 ttcccgcttc ttgacctacg agtgtcaatt gctctatcac ttttaaataa ggaactaaat
      481 gtgaagattg aaaaataaaa ccaatatctt ccaatcgtaa atcactagga cgatgctgtt
      541 tatcaaacaa tggcgcatca ttgtaaagca ctgtaccact cgtttgactt aacaatccgc
      601 ctaatatcgt tagcaatgtt gttttcccag aaccagaggc accatttaaa atgacaaatt
      661 ccccttgttc cacttcaaaa ttaatacctt ttaaaacttt tgtttcagac aaaccttctc
      721 cgaaattttt gacgatatct tcaacgacta atgccattat tctgcacctc caattgcttc
      781 gataggatcc actttaaata atttgataaa tgatagtgag gcacctaaaa tcgctactaa
      841 tataaatatc cccaccatta ataaaatatt ttgcgttgtt aagtaaaaag gcatcgttac
      901 cggcatcata aatgatagtc ctactatgat gatcacagca atacctaccc caattattgt
      961 tagtgttaaa atttgtaaaa ctaacgcact caataaatgt cttgtcttaa taccaattgc
     1021 tttcaaaatg ccaatttgtg atattttttg aatcgtcata acatagaaaa atgcacttag
     1081 aacgattgct gtaatagcaa acaaactaac aatcatcata tttaacggtg cttgctctgc
     1141 ttgataactc gcaatattac ctgttaaatc tttttcactc acaacttgaa cgtcatttat
     1201 tttattaagc tcatcacgtt gttgttgtgt taaatttttc actggataaa atgccgtatt
     1261 aaccttattc aatgcattaa atccgttatc gttcaatagc acaatggaac tatgcgcata
     1321 cattgtgtcg tttaaaatac cagaaactct ataactagtt gaattatttt taaaatgtaa
     1381 tctatccccg actctaatgt tgtctgcagc taacttatca ttaatagcga cctcatgagc
     1441 atttttagga taatcacctt ttttaagttg tatgcgttgt tgtttaacgt cattcattgt
     1501 aatcacatct tgttctttat tagataattt caatgtttga atattcattt tatatggttc
     1561 ttgatgaatt acctttttaa tttgattctg ttgcgtatca ctaagttgcg atttctcaat
     1621 ttgcggctct ttcatttttt gaacaacata ttcatcatta tcaaagtgtt caaataacga
     1681 aatattctcc ctaccaagcc cttgagctaa accactaata aataacacca taatacttaa
     1741 taaaagaatg attaatgtga ttaaaatata acgaaatttg taaaacataa tctcttttat
     1801 cgctaatttc atatcgattc acttctccct atttcttctt tagcgtcagt ataaggttga
     1861 aatatgaact caatatgaac aattgttttt ttacgcaaaa atattttaca tgtataataa
     1921 atgaaaaaga taagggagtt tatagctatg gtgcaatgtc ttgttgtcga tgacgatcct
     1981 cgaatcctaa attatatagc tagccattta caaacagagc acattgatgc atacacacaa
     2041 ccaagtggtg aagctgcatt aaaactttta gaaaaacagc gtgtcgatat tgcagtggta
     2101 gatattatga tggatggtat ggacggcttt caattatgta atacattaaa aaatgattat
     2161 gatataccag ttattatgtt aacagcgcgg gatgcactta gtgacaaaga gcgtgcgttt
     2221 ataagcggta ctgacgatta tgtaaccaaa ccctttgagg ttaaggaact tatttttaga
     2281 attcgtgctg tattacgtcg atataatatc aattcaaatt cagaaatgac tattggcaac
     2341 ttaacgctaa accaatccta tttggaactc caagtatcta ataaaacgat gacgttaccg
     2401 aacaaggaat ttcaattatt atttatgctt gcagcacgtc ctaagcaaat ctttactcgt
     2461 gaacaaataa tagaaaaaat ttggggctat gattatgaag gagatgagcg aacagttgac
     2521 gttcatatta agcgactacg ccaaagatta aaaaaattaa atgccacact tacaattgaa
     2581 acagtaagag gacaaggcta taaggtggag aatcatgttt aaaacactct atgctagaat
     2641 tgcgatttat tccattacgg ttattttatt tagtgcatta ataagttttg tattaacaaa
     2701 tgtttactat cattataatt taaaagcatc taatgacgcg aaaatcatga agacgcttaa
     2761 agaagcaaga caatatgaac aatcagctaa accaacgcac attcaacaat atttcaaaca
     2821 tttagggcaa atgaattacc aaataatgac cattgatcaa aaaggtcata agacatttta
     2881 tggggagcca tttagagaag atactttatc acaaaatgct attaacaacg tattaaataa
     2941 tcaagattac catggtatta aagataaacc attcgcatta ttcgtaactg gatttttcga
     3001 caatgtaaca gataatactg ttggtattaa ctttaagaca aaagacggtt caatagcagt
     3061 atttatgcgt ccagatattg gagaaacatt tagcgagttt agaacattct tagccgtctt
     3121 gttaatgttg ttattattta tctcgatttc attagttatc gcatcaacct attcaatcat
     3181 tcgtccagta aaaaagttaa agctagcgac cgaacgctta attgatggtg attttgaaac
     3241 acctatcaaa caaacacgca aagatgaaat tggaacatta cagtatcact ttaataagat
     3301 gagagagtca ttgggacaag ttgatcaaat gaggcagcat tttgtacaaa atgtatctca
     3361 tgaaatcaaa acaccattaa cacatattca tcatttatta agcgagttac aacaaacttc
     3421 tgataaaaca ttaagacaac aatatataaa tgatatctat accattacaa cgcagctgag
     3481 cggattaaca actgaattgt tgctcttatc cgaattagat aatcaccaac atttattatt
     3541 cgatgataag attcaagtca atcaactcat taaagatatc attcgacatg aacaatttgc
     3601 tgctgatgaa aagtcgttaa tcattttagc tgacctagaa tcaatcaatt tcctaggaaa
     3661 tcaacgtttg ttacatcaag cattgagtaa tttattaata aatgctatta agtatacaga
     3721 tgttggtggt gccattgaca tcgctttgca acatagtcac aacaatatca ttttcacaat
     3781 aagtaatgat ggttcaccaa ttagtccaca agctgaagca cgtttatttg aacgttttta
     3841 taaagtgagc aaacatgata atagcaatgg tttaggtctt gcaattacaa aatccatcat
     3901 tgaattgcat catggtacaa tccaattcac acaaagtaac gagtatgtaa ccacctttac
     3961 tattacactg ccaaataatt cactttaatc tctacctcct gaaaaaacat ttctgttttt
     4021 atttcaggag gtatctttta tattgattag cgctttctag ccacttaaca tttaatttct
     4081 accattcaac aaaatttaaa tatatcgatg gtcatttatg gtttaataat tttgaaggag
     4141 gcattaacct atgatgaaac tcaatttatt tatcaatgca aatgagacgg aatcttatat
     4201 tgatatacat gcacctaaaa tgaatgataa cgttcaaagt attattaatg cagtcaatga
     4261 tttagacaaa tcacatacat tagtcggata catagacaaa gaaatccata tcattaatgt
     4321 atcagatgtt ataacattcc aagttattaa taaaaatgta acagccatta caagcaatca
     4381 aaaattcaaa ctaaaattac gactttatga actagaaaaa cagttaccac aacatttcat
     4441 tcgcatttcc aaatcagaaa tcgtcaacaa gtattacatt gaaaaattat tattagagcc
     4501 aaatggactt atacgtatgt atcttaaaga tgcacactac acctattctt ctagacgtta
     4561 tttaaaatct ataaaggagc gcttatcaat atgaaaaatt tgaaaaactc attatttatt
     4621 agcttaatca ttggtttgtc cctttcactg ttctttagta tgttatttgc agacggcaaa
     4681 tattatccac tgaacccaca atcaactatt ggcatattgt attatacgca tttcacagaa
     4741 actaccgtaa tgttgatttc tatcatttta tggttactca taggcgtcgt ctttttcctt
     4801 ggagatttta tctttaaata cacagattgg agcattacta aagcaactat aatgcatttc
     4861 ataacaacgt atgtcggatt cttgccttta gcaatgcttg ccggttggtt tccactaaca
     4921 gtgcattatc taatcatatt cacaattatc ttcattgtgg tttatgtatt aatttggata
     4981 attcaattct ttaaaaacaa gaactacgta gacactatta acaagcaact gaaacaatta
     5041 aaatgatttt tattaaccac taaaaaacgg taatgtctat cacattaaaa aagacattac
     5101 cgtttcgttt attatttaac ttgtaaatac ttagttactt cttcattaat tttatgatat
     5161 aaatcctcat gatctgttaa gcgataacca aatgacggca ccatttcttt aatctttggt
     5221 tcccatcctt tgaattcatc acgatagcat ctctgtaaaa catcaaacat aatatctaca
     5281 gctgttgacg caccaggtga tgcaccaagt aatgcagcta atgtgccatc atctgacgta
     5341 ataacttcag taccaaattg taagttacct ttagaatcct cagtatcctt gattacttgg
     5401 acacgttgcc ctgctgtaat cacttcccaa tcttcatttt tagcatttgg gaaaaagact
     5461 cttaaatcat ccatacgctc atcattagat aacatcaatt gtgacactaa atacttcgta
     5521 agactcattt ctttgatacc tgcagataac atcgtcacga tattatttgg tttaaccgat
     5581 ttaattaaat ccatatgtga acctgttttt aaaaatttag gtgagaaacc tgcaaatgga
     5641 ccaaataaca atgaacgctt gccgtctaca aaacgtgtat ctaagtgcgg cactgacatt
     5701 ggtggcgcac ccactgctgc ttttccgtac actttagcat gatgacgatc aatcacttct
     5761 tgatttgtac agcgcaggaa taatccactt accgggaaac caccaatatg ttttgattgt
     5821 ttaatcccag tcttttggag taatggtaaa ctcgcaccgc cagcaccaat aaatacaaaa
     5881 tcagattcat atgttgtcgt ttcattagtt tctaaatctt taactttaac tagccatacc
     5941 ccagatttct gtttcttgat gtctaataca ttctgcttat attgaattcc cacaccacgt
     6001 tgctctaaat tatcaaataa ctttgcagtt aacgcaccaa agttaacatc tgtaccagtt
     6061 tcatcataag ttaaagcaat tggttcatca gacttacgac cttcaatcat taacggtacc
     6121 caagatttca tttcttcttc atcttgcgat aatttcattt tttcgaataa aatacttttc
     6181 tttaacgttg caacacgact ttttataaaa gctacattat cttcgccaat gacaaaactc
     6241 atgtgtggca ccgcttgaat aaagcgatct gggttatcta attgtcctgt tttaactaaa
     6301 tatgcccaaa actgttttga aatttggtac tgctcattta tcttaattgc tttactacaa
     6361 tcaactgtgc catccttacc ttcttttgta tagttcaact cgcaaagtgc tgaatgccct
     6421 gtaccggcat tattccatac atttgaactc tcttcgccag gttgtgctaa tttttcaaac
     6481 actttaatat ttttctcagg tgataattct ttaagtaatg ttcctaatgt tgcactcata
     6541 ataccgccac caattaagat gacatctgtt ttgctatgtt gtgttgtcat aacaaataca
     6601 gtcccccttt ttaaaatgaa taatggaacg ttcgagaaat acgacatatt aataccactt
     6661 taaatcaata agtgtctcaa taggttccca tggtgatgct ctattactat ctactatgta
     6721 tattaatatt ttataataac gaaactactt taattatata ctttatgtgc gatagcacaa
     6781 agacaaaacc acaaacctta acagctatca accaatttaa tttgattgct gattactcac
     6841 tattcatttt aacataaagc gctaccatta ttataatgtc aaacttcttt cattaatgat
     6901 ccataaaaaa aagatgagat gttagaaaac atctcacctt ctgtatattt atattttgaa
     6961 atacgttttt ttataataat gacaagatga aagtccaaat acagatgaat attagtaaac
     7021 atacactgta tttcaatgtc atttttaata attctgattc cttaccaact tgttttactg
     7081 ctgcagttgc aattgcaatt gattgtggtg aaatcaattt tgccgctaca ccaccaactg
     7141 tattagcaga tacaagaagt gaaccacttg ttccaatttg ttgtgcaaca gaagcttgaa
     7201 ttggtgcaaa taatgagttg ttatttacaa ctgatcctgt cataaacaca cctatccaac
     7261 ctaaaattgg tgatagaact gggaagacat tacctgcttt agcaatacct tgacccattg
     7321 ctgcacttaa accaccataa gttgtgattt tagaaattgc taagatgaaa caaattgtaa
     7381 gaactggtaa ccacaactct ttaaatgtaa cgccgaataa tctacctgca tctttaaagt
     7441 taaccttttt agacattaaa attgtaataa taatagttaa taaaatagct gtacctgttt
     7501 gtccaataat atttaaagtc aatactaatg gtttatgcgt aacttcgctg attgttccag
     7561 gtaagttgaa tttaaatact aatgatgata aagcaccatt tggtaagaat aagtttttaa
     7621 agaatggcgc actccaaatc attacaatga ctgttaatac aatgaatggg ctccatgcat
     7681 gtaatactgc ttttgcagaa tgtgcttttg caggttcaat ttcttcatct ttattaacac
     7741 gataaatgtg ttttggttgg aattttttag aaaatactgc taatgctaac attgttaata
     7801 acggtggaat aatatctgct aattcaggtc cactgaatac agttaataat ccttgagtaa
     7861 atgtataagt gattgaaact actaaaattg ctggtaatgt ttctttaaca cctctaaaac
     7921 catcaataat aaagataagt aagaatggaa taatgaaatt tatgattgcc aatgttaaag
     7981 ttgctgattg agaaacacct aatactgaaa catctccagg taatttcaac gtttctacaa
     8041 cacctacagg gataccaatc gcaccaaaag caccagacgc tgcatttgcg actaaacata
     8101 acatcgcagc ttttaatgga ttaaatccta attgtgttaa taaaagtgca caaattgcaa
     8161 ttggtacacc aaatcctgct gcaccttcta aaaatgcgtt gaatgcaaat ccaataagta
     8221 aaacttgaat acgttggtct tgtgaaatat ttgtaatact atcttgaatt gttaaaaatt
     8281 gtccagattc aacagtaatt ttgtataaca atactgccat cataacgata tagccaatcg
     8341 gaatgatacc ttggaagaaa ccttcgacta ctgcaccaga agcgatacca actggcaatt
     8401 tgaaaaatgg tattgcaatt agtaatgtta caacaagtgt tgtaatagct gcataaatac
     8461 ctttcatttt aaaaacagtt aaacatagta aaaacagtac aatcggtatt gctgcaatta
     8521 aagacgataa taataaatta tcaaatgggt taaacgtatt tactaacata ggctgactct
     8581 tctttccata aagtagtagt gtaattgctt cattaaaatc atgctctggc tatagcattt
     8641 tgttatcgat aacattttac agaacagtga gattaatgtg atttttttca caattaaaat
     8701 ataccatttt ttcagactat taacaatatc actttcctat ttaattgttt tatttttcat
     8761 tttaaatctg ttcatcaaaa tgaattgtac ttaatgtaat gaagtaactt atactattca
     8821 tgaattttac ataagaccgt gctaaaataa ggaagatact acgatgagga atggaagtta
     8881 tagcaatgat taaacagata aatgtttcta atatgcaaaa gtttgaatct caattaatga
     8941 aagctcagtc tgaaggttat acacatgtcg ttccatatgc caatgagata atgatttatc
     9001 aatcgatgtt agatgcggtt caattatatc ctaagtctat cgtggttgat tatactgtgg
     9061 atggacaata caaaaatgat tgtcactatt tcggtcaatc atcgatcaac attgccgatt
     9121 gggctcaaaa caataattat tatcctaacc tgatatatgc gattcaacag actcttgatt
     9181 taatacatta ttactctgta gaaacgattt tcgatttagc tttattgaca cttctgaaag
     9241 gtgatttatc aatcgatggt catgtcgttt ttgattttaa agcccctata gcaacaagtg
     9301 cttcaatatg ggaaactatt aaaactattg aagattttga tatgatgtct cagttttatt
     9361 tgaataaaat ggcttatatt gatcatcatc ctataccttt tcgcaacctt tttatcgagg
     9421 attcagaaca acttaattcg ccagataatt ggttgtattc taccaagttt atgctaccta
     9481 aatggttata taaaatagca aagcaacgtg ctgataacaa acaattacaa aacttcggcc
     9541 tgtatacgaa gcaaccaaac gttttaaaag atcacatcgt ttttatcggt gatcatcatc
     9601 aatatatagg taattcaaaa tatttattta cttacttcgt taaacataat ccaatgactg
     9661 cctgttattt tgttactgac gatcgtcgcg gaccacattt catatcacct aagtctgaaa
     9721 aagctgacga attgattaac agcgcacgcg tagttatagt tgaaaatgat atacctgaaa
     9781 cgttacaacc aaacggtact ttaattcaat tacatcaagg cacaccaata atgcaattat
     9841 ttttagatag taaagagcct attaaaaata tagaaacacc tttttatcgt gctaaaaggt
     9901 ataatcgctg gttgcaattc gattatgtta tacattctgc tgatgatatt agccactttt
     9961 atcaaacggc ttttcctagt caccaagcaa atgtattggc ttatggtaat cctaaacacc
    10021 aatatttact acaaaagagt aacgaaagta ctactcaaca acagtataag aaatcgttta
    10081 aaataaatga tcaaaaacca gttttattat acgcacctat tggattagtg agtgcgcaac
    10141 aattaccact atcagatgct ttattcaaag cctatcatgt tgtggttcag ggtgttgatg
    10201 aagcgatctt accagaagag gcgcttgttg caccaaagta tcttagtgct caagacttaa
    10261 ttctaatgtc tgatgtcgtt atcactgatt actcaaatat aatatttgat gcgatggcta
    10321 tagacaaaac agtagcatta tacactccaa atcacagtca atacattgag tctcaaggtg
    10381 ttaacgaaga tatttggcgt catctatcaa aaatttggta tacagaccga caattattaa
    10441 ttaataattt aatttcccag gcaataccag tgattaaata tccacaaata caacataaag
    10501 aacaaccatt agaatctatt tcacaactta tattgtctaa gatgacatct aatcaatagt
    10561 gtttaaattt ctcagtggct gtgaatgagg tttaaaagta ctataaaacg taaactttga
    10621 tactttaaaa tacgcaaaaa acggtaaacc ctaattcata ttatagagtt taccgtttta
    10681 ttttttaact tgcatcatag ttatattaac attattgttg gtagtttgga tcagtaacca
    10741 ttgcttgtcc agtataatca accgttacaa ttgaatattt tccatttgca tttgggtctt
    10801 taaaactaaa cacatactta tagttgccat tatgttcttc aatagaataa tcattataca
    10861 ctttattatt actaccaaat ttatttgctt cattattagc cgcatttaaa gctgtttgga
    10921 aatttggcaa ttgctgtaaa gcttgatttt tatttccatt aaacggataa atttgacgtg
    10981 caaccggcgc ggcattttgt ccataatatg gtgcaacgta acttgatttt tgattattat
    11041 tcgcttggtt attacttgat tggttattat ttgtttggtt ttggtcattg tttgttgcat
    11101 ttgaattaga ttgttgctgg ttatcgtttg cactattatc tttattatct ttgtttacgt
    11161 ctttactatc atctttatta tctttcttat ctttagatga atcatttgtt tttttatctt
    11221 gttgttcagt tttcgcttta tcatcttttt ctttattacc gtctttttgt tggtcactat
    11281 cttgaccaca tgcagctaaa aataatgata atgctagtaa ccctgtaact aatcttttca
    11341 tacatatctc ctcctataat tcgatattca ttgaataatc ttgaaataca tatctaccat
    11401 gtgtatcttt tcatggcttg ttaccaaaga cgtcaacatt aatatgatct gaattatcac
    11461 aatatccata actacaatct aaggttcttt ttattatacc ctaatttttg ttcattatta
    11521 tttaattttt gtgaatttta tgttttctat aaatttaatt attttacttt aacaattcat
    11581 tacgcattta gcatttcaag gtatacacaa tatttattac tatgatttca ttttatctgc
    11641 tgcaaaaaca atcattataa ctctttttcc ataattaaat ctgtatccgt tacatcacct
    11701 gtttgaaaat gatgttcacc aaccacttta aatccatgac gtttataaaa tgcttgagca
    11761 cgaggattat gctcccaaac tcctagccaa attttatgtt tattatgttc ttgagcaatt
    11821 ttttcggcca attctatcaa ttgtgaacct cttccgccac cttgaaagtc tttcaaaaaa
    11881 tatatgcgct gcacttctaa ataggtctcc cccatttctt cagtttgagc actattaata
    11941 ttcatcttta tataaccaac attcgcacca tcttcttgat aaaaataatg aaatgaatct
//
LOCUS       DAKTIP010000069        11861 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAKTIP010000069 DAKTIP010000000
VERSION     DAKTIP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.069"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            15..1049
                     /locus_tag="O0305_001392"
     CDS             15..1049
                     /locus_tag="O0305_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0274541.1"
                     /translation="MLTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFYSLLAILL
                     ILLLASFIKATRANNDSKEAVNKTNMIQKKYEDNANTVQYSPKLKLYADKFIDTYMNI
                     SKDSKELESREKELLKYFPSDYKKPDEKISDTERKLNSKEFYNIKRKDKQTIIQYIVN
                     YDVNITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPIKSENNKYVVVE
                     YPYFTPIPDSQLNQAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASSKSDDMAYLMDN
                     PEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYTLDITKKDGKYY
                     VKNMTNSIGG"
     gene            1054..1314
                     /locus_tag="O0305_001393"
     CDS             1054..1314
                     /locus_tag="O0305_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274542.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            1326..1709
                     /locus_tag="O0305_001394"
     CDS             1326..1709
                     /locus_tag="O0305_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCY0274543.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1744..4239
                     /locus_tag="O0305_001395"
     CDS             1744..4239
                     /locus_tag="O0305_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0274544.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESKEMQEKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
     gene            4293..5651
                     /locus_tag="O0305_001396"
     CDS             4293..5651
                     /locus_tag="O0305_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCY0274545.1"
                     /translation="MAMNEIALFKGVRIQPYQKYIDFMFASVLAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
     gene            5656..7503
                     /locus_tag="O0305_001397"
     CDS             5656..7503
                     /locus_tag="O0305_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274546.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNIIMNSTSSIDGNIDYIHENSGMNHIRNQLFD
                     MTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDYDKKSEKELDDIVKDEVEKKNNK
                     YMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKLIFSTFALFLFLFLVFSWIVSFI
                     PGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIKLANSFIEPDSQNAYFLNSIFII
                     VILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNRTQERFNKIRHEQAQNKKAKREN
                     QRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNGNKRREQQESMNGNDVKSASVES
                     NANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVNQPLNNENHSINRKEQKSVQTAY
                     DTDVQKRQIQNATQNQQSRQSGNRNQPITRNSQSKDRLKEQKDINKHGK"
     gene            7493..8539
                     /locus_tag="O0305_001398"
     CDS             7493..8539
                     /locus_tag="O0305_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0274547.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            8546..9136
                     /locus_tag="O0305_001399"
     CDS             8546..9136
                     /locus_tag="O0305_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274548.1"
                     /translation="MKQKIIMFTLVTVILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDITVNDIHVYFDTYDPKKDSYKVFVQFDERIETDGD
                     DKIEHRQTSAQLDLVRTAEGWRIDNLKRFNLKPLGR"
     gene            9192..9548
                     /locus_tag="O0305_001400"
     CDS             9192..9548
                     /locus_tag="O0305_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCY0274549.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            9657..10160
                     /locus_tag="O0305_001401"
     CDS             9657..10160
                     /locus_tag="O0305_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274550.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            10141..10677
                     /locus_tag="O0305_001402"
     CDS             10141..10677
                     /locus_tag="O0305_001402"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0274551.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEEIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
     gene            10897..11445
                     /locus_tag="O0305_001403"
                     /pseudo
     CDS             10897..11445
                     /locus_tag="O0305_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         4338 a         1320 c         2198 g         4005 t
ORIGIN      
        1 taataagcaa gtacatttta acacgatttt ctaaaaacgg aaagcatttt gatattccta
       61 aagaacaacg ccgagtaatt ccaaaattta aaaatcgtcg taagatgatt gtaatttgtt
      121 tttactcatt attagctatt ttgttaattc ttttattagc ttcttttatt aaagctacaa
      181 gagcaaataa tgacagtaaa gaagcagtta ataaaacgaa tatgattcaa aaaaagtatg
      241 aagataatgc gaatacagtt cagtatagtc ctaaattaaa gttatatgct gataagttta
      301 ttgatacgta tatgaatatc tcgaaagatt caaaagaatt ggaatctaga gaaaaagaat
      361 tgttgaaata ttttccatca gattataaaa aacctgatga aaaaatttca gatacagaac
      421 gaaaattgaa tagtaaagaa ttttataata ttaaacgtaa agataaacaa acaattatac
      481 agtatattgt taattatgat gtgaatatta ctgaaaagaa agaagttaaa gttaagaaga
      541 aaaagaaaag tgaaaaagat aaagatgagt atgaaacaaa aacagaagaa aagcaacgta
      601 aagtaaatca aaacatttta ataaatatac ctattaagag tgaaaataat aagtatgttg
      661 ttgttgaata tccttatttt acgccaatcc ctgatagtca attgaatcaa gctaaaatgg
      721 ttgaggataa tttgaaagac aataaacggg aagacaatcc gaaagcaaaa gcttttattg
      781 aagatttctt taataaatat gcttctagta agtctgatga tatggcctat ttaatggata
      841 accctgaggg cttagaagga actagagaag tttctcagat aagagaaatt aggttatatc
      901 caaaaggcga tgattatgtt gctaaagttg aaattttgat gaaagataaa gattctcctt
      961 tggagaattt ggagcattac acattagata taacaaaaaa agatggtaaa tattacgtta
     1021 aaaatatgac taattctatt ggaggttaat gttatgagtg gtttatttaa tttttttgtt
     1081 ttaggtgctg accgtccatc acttggtgga gttggtgact ggataagtaa tgaagtaggt
     1141 acgggtattg gtttagtagt attggttgtt ggtattgtaa aatgggctag tggtaaatat
     1201 gggcatatgg ttatattatt tgttgttggt ggctttttat tcttagtttc taaaggtcct
     1261 gaacaggtat ttaatgcgat tgcaggtgtt tggaaaatga tttttggcgg ttgaggtgat
     1321 tccatttgga taaaaaagct tataatttaa agcgaacttt tgaacaaccg atagttatgt
     1381 atgaatttac agataagttt cgtattaata agggttttcg tttagatttt tgggctacat
     1441 ttttgattgt gtggtttata ttatttttgt tattttggta tttattacag cctgtaatta
     1501 tgtcgattgg tggacttatg tttatttact ttacggttgc accttattat ataacgaaat
     1561 atattgttaa gttaaaacaa gatggaaaga aattattttt cttcttatgg gattttgtaa
     1621 tatttgtttt taatgtgcag ttaagaaaag ttaaattaag ttatgatgaa gaagtagaat
     1681 atcatgataa aaaaataaca tttaaatagc ccctattggg gttattttta ttggagggat
     1741 gacatgaatt taaaagcacc gtatgcaggt ttaagagata atttgttatt aacgaaaact
     1801 ggcgaagttt gggcgtatta tcgtgttcgt tctgagtcta tatcaacagc aaattatgat
     1861 gcaaaagaag aatcaaaaaa gcgtatgcgt tattttttag agtcgattaa aggttatcaa
     1921 gattttcatt ttgaaatgta tgaccacgat ttagatttgc gaaataaatt taaagaaatt
     1981 agtaaagatt ttgatgatga agcttttaat gtagctgaat attattcaaa agaaactatt
     2041 gacgtattag aacaagaatt acaaatgatt acgcataatt cttttgttat gggtgttaaa
     2101 atacgtgaat tagctgacga tgcagttaca cttaaagaga ttttaaaaag tacattatca
     2161 gatacggctg agcgtattgt ttcgaatttt ggttttgatg tgaatttaga cgataaaatt
     2221 ttagataagt ataaaacatt tgaacgagat ttggctgata gttttctagg tattggaggc
     2281 gagcgtttaa ctgagaatga attggcttat attattagaa agccttttat ttcaaatgca
     2341 acacatgacg tacaggaaga atcttcaaga agagctatta cagatattta taatgcagta
     2401 attgacccta ctcgcttgag tgttttagaa gttgaaaatg ataacgagaa attttataca
     2461 acaacagttg ttgttgatga ttttccgtta gatatggaat atacacattt atttgagaaa
     2521 gctcaaaata tgccttttcc ggttgaatgc catgtaaaag caaaaatcat taataatgag
     2581 aaagttaaga aaaaatttga ccgtgcgaaa ataaatcata aacaacaagc aaaagaaaag
     2641 caagatactg gcgacagtgc ttcggaagat gttcaagata atttgtttgt attgaatcaa
     2701 atggaacgag aagtgactgg ctcaggtgta tttattgagt gggtatttat gtttgtaatt
     2761 aaaagtgatg attatagaga ttgtaaacgt aaagcaaagc gtttgatgaa acgattaaag
     2821 gaaactcaaa tatatgcagt taatccatta gctgaccagt tacaattgtt ttatcaatgt
     2881 ttacatggta atgatttatt tattaataag tattggttac aaagaacaac ggcaacaggt
     2941 atagcagaga atttatttgg tgtgtctcaa agtttgggaa caaaaacggg tttttatata
     3001 gggcgtattg ataaatttat acgttctgtt tcacgtgagg aagctgttgc aagttcaagg
     3061 gatataattt tgtttagttt attgttggcc gcaaaaggta ttaagggggc tgtcagtgat
     3121 agtccgcatg ttttaattac tggtcaaact gggaaaggta aatcattttt agcaaaatta
     3181 ttatggattt atacatcatt ttttaaaggt caaatgttgt actgggatcc aaaatctgag
     3241 tttgcagaat ggtttgacag agtaacagaa agtaaggaaa tgcaagaaaa gtatccccta
     3301 tttattaatc atttaaaaac atttaaatat gtaacattga attatgaaga ttcaaccaat
     3361 tatggttgtc tagaccctat tgtattttta gatggtattg aagctaatga gatgttgaaa
     3421 tcagtgtttg atgaaattaa atcttttgaa aattatcatc atattgaaac agcaatttat
     3481 caagctattt ctgatacggt tgaagaacgt gaaaatggta aaaaagttgg ttcattaaat
     3541 gtaattgaaa aattacagaa taatgaagat gagcatgtaa aaaatgcagg cgatttatta
     3601 tttgaaaaaa cgcaaaacaa tattttaaaa cttgttttta gtgatggtac gaatccagct
     3661 ttaaatattc aagaaaaagc aactatttta caggttaaag gtttagatat gcctaaagca
     3721 gatgatgata catcaagtta ttcaacaagt gagaaaaatg ggattacatt aatgttatta
     3781 attggtaagt ttttagagaa attcggttct cgtagagatg ttcaaacgac tatatttatt
     3841 gatgagggtt gggcttttag tgcttcaaga caaggtaaaa aagttggtaa gcgtataaaa
     3901 agaacgggac gttctgagaa taattcactt gtatttatta cacaatcggt aaaagataaa
     3961 gcagatgatg atggtggtaa ctttggttgt cattttgcat ttgatgaaaa agatgaacgt
     4021 gaggatattt taaaatcttt aggcttagaa tattcaaaag aatctcctga aaacatggag
     4081 atgttgaaag atttgaagaa aggtcaatgt atattcagtg atttttatgg acgtgttgga
     4141 aaaatggttg tacattgtcc atttgaggaa atgactgaag catttagaac acaagaagat
     4201 tctgcaagct ctaaagctga agaaaaattt gcgatgtaat ttttagcaca tacaaaattg
     4261 tatgtgcttt ttattttaaa ggagtggatt tgatggcaat gaatgaaatt gctttattta
     4321 aaggcgtacg tatacagcct tatcagaagt atattgattt tatgtttgca agtgtattag
     4381 catttatttt tttggtatat cgtatatata ttttgtttat tgattataaa tcagttaata
     4441 aaaaattgga tatattgacg ttgtttgcat attttaaacc gcatttattg tatttattaa
     4501 ttggtacagc aattatattt gtgatatgta agcttattgc tcagttttta atacgtttta
     4561 aaacaaaaga tttggctgaa atgattgaaa cacaaggttt tcataatcgt acagtaatca
     4621 agaaaacaac tgagtatatc cctattgatt ataaacaaac tgaatatgat tattatccaa
     4681 caatgtttta taagcgtggt aaaaaatctt ttgttattca tattaagaaa gacggttcga
     4741 ggtttcaaga taattattta gagttagaga cgattattga accaatgttt aattctgaat
     4801 tagtagaaaa aaagcatatt ggtcgttatt tgagatatga atttatgcca cttaaatata
     4861 aaaagcgtat tgttatgaat ggcgaacaag aatcaaatac aattttttat gatacaaaaa
     4921 ttgctattac gcatcaaatt acttgggatt ttgtgaaagc acctcatgga ttggttacag
     4981 gtataaccgg aggtggtaaa acatattttt tattttatgt gattcgtgag ttattccgta
     5041 gacattcaga agtacgttta ttagacccaa aagtttcaga tttgagcttt atgaagcgcg
     5101 ttattggtga tgataaagtt gcagatacta aaggtcaaat attaaaacaa ttgagagaag
     5161 ctaataatga aatggaagag cgttttagat taatgaatga tagttcggat tataaaatcg
     5221 ggaatgattt ccgtaatttt gatatgcgcc cctatttcat tatttttgat gaggtaacag
     5281 cttttacttc aactttagat aaaaaagaat tacaagaaat gaatgattat ttaattaata
     5341 ttattatgaa aggtcgtcaa gctggtgtat ttatgttttt aacagcacaa agaccagatg
     5401 cagatgtgat taaaggtaat gtgcgtgacc aattagggtt acgtgtgtct ttaggtaatt
     5461 tgtcaaatga tggttatcgt atgacctttg gtcaaactga taaagaattt caaacgattc
     5521 atgatagtga tatcggccga ggttatattt ctatacttgg acaatataat gaaccaattt
     5581 tatttgatgc acctttaatg gaacaatatg attttgtgga agatgtgaaa caaatattaa
     5641 ataaggagtg atttgatgaa gcataaaagt gtgtatcttt tatgcttttt aattttgcct
     5701 attctgcttt taacatctgt gagtgcttat gctgtttcta atccagttgg agagccctca
     5761 catgcagtta agccaaaaat tgaaaagtat gatttatcac attatcgttc aatatatagt
     5821 gaaaaaggcg actggaatcc gtttggtcaa gaagaaattt cacgtcaaat taataatgtg
     5881 tcagattttt tcttttctat gactaagatt ttagcaggtg ttacagatta tgcgattgaa
     5941 aatttatttc aattagatgt tattaatgat tttgcggata aaatcggcga ttttgttggc
     6001 gatatatatc aaaagttatt gagtaattta gcattaacat tatttattat tgtttgtttt
     6061 aacgcattta ttatttttag tgtacaaggt aatgcaagag aagcattaaa acgagcgttt
     6121 ttaattatgt gtttaattgg ttttggtgtt ggtatacttg cgaatgcagg taatattata
     6181 cgtggtacaa acaatattgg taaggactta aataatataa ttatgaatag tacatcttct
     6241 attgatggta atattgatta tattcatgag aattcaggta tgaatcatat acgtaatcaa
     6301 ttatttgata tgacgatata tagaacatat ttagtaatga attatggaac tgtcgacgaa
     6361 aaagagataa aagcaaaagg taaagaccgt attgataaca ttttaaaact tgattatgat
     6421 aaaaaatcag aaaaagaatt agatgatatt gttaaagatg aggttgaaaa gaaaaataat
     6481 aaatatatga cgcaaggtta tgtatttcaa aagttggcta tttcagtgat tggttttatt
     6541 attacacttt ttatgtcgat agtattttta tcaataagtt ttgcgaagtt gattttttct
     6601 acatttgctt tatttttatt cttattttta gtattcagtt ggatagtgag ttttatacca
     6661 ggatttgaat tatctgtttt ctctgcgttt gcaaaaacgt taggctatat tattttaagt
     6721 gcttgtatga cattcttatt tgtgatagtt ggtttgtgta tcaagcttgc aaatagtttt
     6781 attgaaccgg atagtcaaaa tgcttatttt ttaaattcaa tatttataat tgtaatattg
     6841 tttgtaatgt ataaaaaacg ggctcaaatt atcaattttg taagtcgtgg taatattagt
     6901 ttttctccta gtgcaattgg tgctggtgtg atgaatcgaa cacaagaacg ttttaataag
     6961 attcgtcatg aacaagcgca aaataagaaa gctaaacgag aaaatcaaag agatgaaccc
     7021 gccccaccac ttcaaaatga taatgattta agaagaagac aacaggataa accaatgcct
     7081 ttatttataa ataaagataa tcaaaaaaat ggtaataagc gcagagaaca acaagagtca
     7141 atgaatggta acgatgtaaa atctgcttct gttgaatcaa atgcaaataa ttattctaaa
     7201 caaccacaaa aagcatcaca gcaagaacat caagtacgag aaacaagaca gcgaaaagat
     7261 atacaaaggt cgccacaagt ggttaatcag ccattaaata atgagaatca ttcaataaat
     7321 cgtaaagaac agaagtcagt tcaaactgct tatgatactg atgttcaaaa acgacaaata
     7381 caaaatgcga cacagaatca acaaagtaga caatctggaa atcgtaatca acctattacg
     7441 cgtaattctc agtcaaaaga ccgtttaaaa gagcaaaagg atatcaataa gcatggtaaa
     7501 taaaaaagat attgctaaaa aagtaatagg taaaacgcca ataggcgtta aattgaaatt
     7561 agcgaaagtc atcatcattt tatgtgtggt ggcttttttt atatttccag taattataat
     7621 gtttttgtta gcgcctgatt taaataaagg taaagatgat gctggttgta ctgttagtgg
     7681 cggtaatgtt tcggcaaatg gtattgataa gtttaacgaa aatgctaaag gtggaaaatt
     7741 agagggtaaa ggaaaagaaa tacagaaaat tgcagaaaaa aataaagtac ctgtgaatat
     7801 ttttatggcc attattgcga gtgaatcaca atggggtaaa ggagaaaatg cgacaagaca
     7861 aaataatcct ttgtctgtta tgggttcaaa atcgattcat gattcaacat atccaactat
     7921 tgaagatggt cttaatgcgg gagctaaaaa tttgtatgat gtttatattt ctaaaggttt
     7981 agatactcct aaaaaaatag gtcccaaata tgcgcctgtt ggtgcgtcaa atgaccctaa
     8041 taatatgaat gcccgttgga taccaacagt tgaaaaaatt atgaaagatt taggcggttc
     8101 agaagctaaa acgtcatgta gtaatggtaa aggaaagtct ataaagttta atggtaaatt
     8161 gccacattgg tcaaatgatg accctggaaa aggtaattta tacactgctg gtcaatgtac
     8221 ttggtatgca tacggtatgc gtcaaaaaat gggtaaacct gtatcgactt attggcatga
     8281 tgcacataaa tggaatgata gagctaaagc tgagggttat aaagttgata aaaatccgga
     8341 acctggtgca ttatttattg ctgaacaagg tgcaggcggt catgatgggc attatggtca
     8401 tgtggctgtt gttattggtg taagtgatgg tggtaaaaca tttagaataa cagaaatgaa
     8461 ttgggaaggt gcttttaaag ttaatgaacg tacattgaaa atgactgacg gatatagttt
     8521 tattcatgat aaggagtgaa aataaatgaa gcaaaaaatt attatgttta ctcttgtaac
     8581 agtaatatta ttttgtgctg ttttaattgg ttatcagatt cctaaacaac aagttaaaat
     8641 gaaacaaaat caaattgaag atttgcagga agaacaacgt atattaagag ataaaaatgg
     8701 agagttaaat aaattagtaa agagacaatc aaaaacagtt atttctgatg aagaaaaaca
     8761 aatacgtgaa gtttcttcaa attttgttaa acagatgttt gaaatgaaaa aagattcttc
     8821 ttttaaatca aaagcaccac aaataaagcc tttagttaca aaggattatt acgatacatt
     8881 atttaaggat tctaaagata aatatgattt atatgatgat ataacggtta atgatattca
     8941 tgtttatttt gatacttatg accctaaaaa agatagttat aaagtttttg ttcagttcga
     9001 cgaacgtatt gaaacagatg gagatgataa aattgaacat cgtcaaacat ctgcacaatt
     9061 ggatttagtt cgtacagctg aaggttggcg tattgataat ttaaaacgtt tcaatttaaa
     9121 acctctagga cgttagtttg tttcaagacg atagcaatga tataatttac gtaaaaagga
     9181 gtgaatttat catgaggcga tggtttgtat taattttagg attagttata ttattatctg
     9241 catgtggtca gaaatatgat aaagaaattg atgaagttac aaagttagaa aaagaaagta
     9301 ttcaagatgt gaaaaataca aagaagtata aaaatgttga gcggtcaaaa agttattata
     9361 agatttataa tgatggtgag gttattataa tgacttatat gccttttaaa gatagtaata
     9421 caaaagtttc aagagtatat aaaataaatc agactagcga taaatatgaa gaagattcta
     9481 atattgatgc tgaaaagttt gagaaagata ataaaccagt ttatgaagaa aataatatga
     9541 aaaaataaag aatcaactcc ctatacgctt tagcgatggt atcgggagtt gatgaacata
     9601 tatcctctta taacttattt tataaataac ctttttaaaa gtaaaggagg atatacatga
     9661 aaaaaagaaa aaaacgtaaa gaattagact ataaaacacg tctagctatc gcaaagtatt
     9721 taaatgatgg tttaaacatc attcagattt ctaagatttt aggtcgtgat gatgcagttc
     9781 tttatagaga agtaaataag cgttctgttg atggtgttta taatccttta ttagctgagg
     9841 aagatgctat aaaatcgcgt agtatttcag atgaacaagt tgctaaacgt agacgtttga
     9901 atgatgaaac aaaagcatat attgaagaaa aactttcatt aaaatggtct cctagacaga
     9961 tagcaagtca aattgaaaaa gatacagggt attatataag ttatcctact atatatagat
    10021 atattcgcaa tggattggta aaagttgatg ttaagcgtga tatgagacaa gcaggcaaga
    10081 aatataataa gtcttctgaa aagcgtggca aattagacgt agggtctcgt gttataaagt
    10141 atcggctcaa aaagtattaa taatcgtgaa gaatttggac attatgaaat tgatactgtt
    10201 tggagtgtta gaccatcaac atattgcttg ttaactatta ttgaaagaaa gacaagatat
    10261 ttatatgcta gtagattaaa tacacgtaaa tccaatgtag tttgtaatga gattataaat
    10321 attatgaaac cattattacc caaaagtata actatggata gaggtaagga gtttgctcta
    10381 tttgaagaaa tagaagatga attgaattgt agtatttatt ttagtgattt gggttgtcca
    10441 tatcaacgtg gttctattga aaatgcaaat ggacttttaa gacagtatta tcctaaaggt
    10501 acggattttg caaatataac acaaaagtca ttagatgaag ctgttaaaca aattaatgca
    10561 cggcctcgaa tgatatttga ctataaatcg agtgaggaaa tgctaaagta ccatgtaagc
    10621 acacaaaatt gtgaacctat attaaatgac tgtgtgaggc acgaacccgt caattaatat
    10681 aggttaatct aagcgaatgg tttacaatga gcgcggaagg acaggacgaa taaattttgt
    10741 atgtaaaatg cataatctaa aaagaagcat actatttaaa tctgtggttt ttgacagata
    10801 ttactagtaa aatgcttctt tttcttcttc tgtactcttt tagtaatatt tattaaaaaa
    10861 atttaagaaa ttctcatttt tctattgaca cactgccatt taattaaagc aattggagta
    10921 ttgttactca ttgtcggcat cgtcataatg aatcaattta acaagaataa tttattacta
    10981 actgatcaaa agtatttact gttttggctt ctattaggat ttatttttgg tttctttcca
    11041 cctattcaaa cgacaattaa tagtgcttta gctagtcata ctcattcacc agcctttgca
    11101 tcattagtat catttacaat tgggtcaata gcgctattga ttttaaccgc tatttttaat
    11161 cgttctttaa aactaaaaac aagtcattta aaattcggta aattaaagcc tatctatttt
    11221 actggcggta tacttggcat ggcttttgta acagctaaca ttatcttaat gcctcatatg
    11281 ggtgcagcat taacaacact tattgggatg tttggccaga ttctaatggg catattgata
    11341 gatcactttg gattatttgg ttcacctaaa atagcaatga catccagaaa aactattggt
    11401 ctattatgta ttttgacagg cattatactt ttaagattat tttaaattaa cttttagctt
    11461 atcattttaa cttgtaatta tttttaaaag tgataagcta tttttttgtg gtctaaaaat
    11521 ctttagaaaa gcgttgattt gggtagtatg ctttgacaca acaaatttta atttagcaaa
    11581 ttcgatagtc aactcattct taagacctaa attaatgtta ttttttaata atttacacca
    11641 aattaatagc aaaaattatg ttattcgtgc taatatttca tagttggtta ttcaattaat
    11701 taaaaataag tcaaaatgca caacttttta taattcattg agtcgagttt gaaaaataaa
    11761 agtgctttaa tgcatgatca attatcgtac tttctattat ttgttacccg ttatcaatcg
    11821 gaataacgta tagacacttt aacgtgctat agattggttt t
//
LOCUS       DAKTIP010000070        11833 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAKTIP010000070 DAKTIP010000000
VERSION     DAKTIP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.070"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(86..778)
                     /locus_tag="O0305_001404"
     CDS             complement(86..778)
                     /locus_tag="O0305_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCY0274552.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1163..1960
                     /locus_tag="O0305_001405"
     CDS             1163..1960
                     /locus_tag="O0305_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0274553.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2093..3040
                     /locus_tag="O0305_001406"
     CDS             2093..3040
                     /locus_tag="O0305_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCY0274554.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            3642..6596
                     /gene="fdhF"
                     /locus_tag="O0305_001407"
     CDS             3642..6596
                     /gene="fdhF"
                     /locus_tag="O0305_001407"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCY0274555.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPESELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRQPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            6596..7069
                     /locus_tag="O0305_001408"
     CDS             6596..7069
                     /locus_tag="O0305_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCY0274556.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            7297..7530
                     /locus_tag="O0305_001409"
     CDS             7297..7530
                     /locus_tag="O0305_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274557.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            7552..8328
                     /locus_tag="O0305_001410"
     CDS             7552..8328
                     /locus_tag="O0305_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0274558.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
     gene            8639..9763
                     /locus_tag="O0305_001411"
     CDS             8639..9763
                     /locus_tag="O0305_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCY0274559.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLFVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(9855..10808)
                     /locus_tag="O0305_001412"
     CDS             complement(9855..10808)
                     /locus_tag="O0305_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCY0274560.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(11170..11661)
                     /locus_tag="O0305_001413"
     CDS             complement(11170..11661)
                     /locus_tag="O0305_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0274561.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHT
                     PSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNY
                     IHI"
BASE COUNT         4194 a         1645 c         2237 g         3757 t
ORIGIN      
        1 gcgaagccat tcaatacgaa gtattgtata aatagagaac agcagcaaga tattttctaa
       61 ttgaaaatca tcttactgct gttttttagg aatcgatggg gtttaaaaat tgatgatagt
      121 tatcaatgga tatgtctctg gtttgaatcg tcttaccgaa tgaatacgaa atatctataa
      181 tattttcaat ttcgagttcg gcagttttgt cttgagcaat gtcataaatg actgcatgcc
      241 aatgtccatg tttacacaaa aggccaataa atataacgtt ttcagcttta atattatcat
      301 caaccctatg atctactaac atcacatttc gttctgtaca ataaattaaa atgtcggaaa
      361 acagcattgg gagtgtcata tgttgtgtgt catcaaattt aatataaaag tccatgcgtt
      421 taagcaattt tcttaagcgt gtttgtggaa gacttaaaca ttgttttata attgcgttga
      481 tttcgctttt atatggtaat gtcgagtacg actgactttc attaagcact aaaaataagg
      541 ctgataattg tgtttcagat aagtttaatg caattttaga ttgtgcttgt tgtatttggt
      601 aaccaccatt tttcccataa tgcgcataaa ttttaacacc ttgattttct aaatcatcaa
      661 tatctcttaa aattgtgcgt ttggatacgt tgcaatattt agctaattct aaagcggtca
      721 tttttttatt ttgttgaatt gcagtaatta ttaaattttg cctttcagct ttattcatga
      781 cagccacctc ctaaaagatg atatattaca caaagtataa cataagagtg acaatgagga
      841 cattttcaat atcttttaaa aaaaatcgta aaaattttgt ggaaaacgct tacattttaa
      901 aaaagattgt gctatgatat tacttgtaac agggggagcg attaaacaaa ggggtagagc
      961 tatataacag atagctttta atcgttcaag ttacatcaca acatcttcaa tatttattac
     1021 ttactttcct ttctatttgt cggctagcac gtgactagcc gattttttta tgcataaaat
     1081 gaagaagtct tcagttgtat tgttgattct tttaatatag ggtaataata aaatgataat
     1141 gatatgagaa gtggtggacg aaatgacaga taaaacatta cagcaaatag ataagttaat
     1201 atgttcgtgg ttgaaacaaa tagacaatgt tattccgcaa ttaattatgg aaatgactac
     1261 agaaactaag cgtcatcgat ttgatttagt tacaaatgta gataaacaga ttcaacaaca
     1321 attccaacaa tttttagcaa cacattttcc ggaacaccaa ctattagcgg aagaaaaaag
     1381 taatgaaatg attacgaatg aaattaatca tttatggatt atggatccca ttgatggaac
     1441 tgccaattta gtaaaacagc aagaagatta ttgtattata ttggcgtatt tttatgaagg
     1501 taaaccaatg ttatcgtacg tatatgatta tccgcataaa aagctttata aagcaatacg
     1561 aggagaaggt gctttttgta atggaattaa gatggaagaa ccaccatcgt taaaattgga
     1621 agacgctatt atatcgttta atgcacaagt gatgaatcta gatacggtgc aagatttatt
     1681 tgacgcctca tttagttatc gtttagttgg tgcttgcgga ttagattcta tgcgagtggc
     1741 aaaaggtcaa tttggggcac atattaatac aaatcctaaa ccttgggaca ttgctgcaca
     1801 gtttttattt gcagaactat tgaatctaaa aatgacgact ttagatggta aggcaattga
     1861 tcatttaaag ggtgcacctt ttattataag taataaagca tgtcatgaaa cggtacttaa
     1921 aattttaaat gcaaatggtg gttatcaaaa atacagatag atattctcta gagataaaaa
     1981 atgataaaaa atagttatac gtctacagaa gtatagtaaa aaaattattt tactatataa
     2041 taaatagaga aaacagacaa agtatgtaag gaatagtaaa ggagtggtgg gattgaaacg
     2101 ttcgtctaaa agtaaaatga gcctaccgaa aaaaatattt ttatgggtat tcggtatttt
     2161 agtaatctta gcgattgtag ctgttgtgta tgtagctgct aaaattttta ttactggtaa
     2221 taagatacac aatccgttag atcgcaatca ttcagaatta agagataaaa aagtgagttt
     2281 aaacgatggt gatccattca caatcgcttt atttggtgtc gattcagatg ctgatcgtaa
     2341 gaaaaagggt ggcggagagc gtagtgatag tattatgatt ttatctatca accctaaaac
     2401 gaagaaaact gaaatagtaa gtataccgcg tgatacaaga gcggaaattg ttggccgtgg
     2461 tactactgaa aaaattgcac atgcctatgc atatggtgga ccaaatatgg ctgttaaatc
     2521 acttgaaaaa ttaatgaatg tgccaattga tcattatgcg acaatcgata tggatggttt
     2581 gcataatatg atagatagca tcggcggtgt tgatgtagta agtaatgata cattcactgt
     2641 tgatggtgtt cgttttacaa aaggacagca gacacatgta aatggtgatc aagcattgaa
     2701 gtttattaga agtcgtaaag aagaaggtgc tggtggtgac tttggtcgac aacaacgtca
     2761 gcaaattgtg cttgaagcga tggccaataa gattgcaagc ccatcatcga ttacacattt
     2821 taatagtcta atgaatgaaa ttcaaaataa tgttaaaaca gatttaacat taggtgattt
     2881 gaatacgatt agaagcaatt ataaagatgc taatgacacg attaataaac atcaattgag
     2941 tggtcaaggc ggcattcaaa gtgacggatt gtactacttc attcctagtg agcagtcaaa
     3001 agctgaaagt actaagttat taaaagataa tctagagtag taaactaagg gaaaataagt
     3061 tatcaaaatt gtacacaaat taattagata gaatgatctg taaatagaat aacgaataga
     3121 actaatagcc cgtaaataga aggtgataac actttgtatt tatgggctat tttatttaat
     3181 gcctgatttg atttttataa attaatccat tacaaattta atttaaagat aaatattacc
     3241 ttctaaatga tagtagatat ttatcaaact tgggttatta attttcagaa aaagggtatt
     3301 tataaatagt ataacgattt ttaggtgagg tgattattaa atattgtcat taataaggtt
     3361 gtgaaagata tggcaaaaag cgtgaaacag ataattggaa aactaataat gataattatt
     3421 ttcaattaaa caaaaagatg atttaatttc gcgcaaaata tttcaaataa aaatgattgt
     3481 aaaaaggcaa aatacaaaat tttacttaac aactagtaca taaagtaata caattaaatt
     3541 aattctatct gaaagatgtg tggggcatcg ttattttagg tggatatgag caatttatta
     3601 aaagtcattt acggaaaata tatatagacg gggtgagtaa tatgcaagaa catttggtgg
     3661 ttacacttga tggaaaagat tatcttgtag aaccaggtac gaatttactt gaatttatta
     3721 aatcacaaga tacttttgta ccttcaattt gttataacga gtcgatggga ccaattcaaa
     3781 catgtgatac atgtactgtt gagattgacg gtaaaattga acgttcatgt agtacggtga
     3841 ttgatcgtcc aatgactgta aatactgtga acaatgatgt gaaagatgct caaaaagaag
     3901 cgcttgatcg aattttagaa aagcatatgc tgtattgtac agtatgtgat tataataatg
     3961 gtgattgtga aattcataat acgatggatg catgggggct tcagcatcaa acgtatgaat
     4021 ataaagagaa gccatacgaa aaagattacg gcccatttta tcgttatgat ccaaatcaat
     4081 gtatcttatg tgggcgttgt gtagaagcat gtcaagatat cgaagtgaat gaaactatta
     4141 gaattgattg ggatcgtgaa catccacgtg ttatttggga taatgatgta ccgattaatg
     4201 agtcttcatg tgtatcttgt ggtcaatgtg cgacggtatg tccatgtaat gccatgatgg
     4261 aagtgaatat ggaaggtaat gcgggttata tgactgatac ggaacctggt tcattagcag
     4321 caatgattga tttaactaaa aaagcagaac ctggttatgg tccactattt gcaatctcag
     4381 attcagaagc agaaatgcgt aaagagcgta ttaagaaaac taaaacagtg tgtacttatt
     4441 gtggtgtggg ttgttcattt gaagtttgga ctaaagatag agaaatttta aaagtacaac
     4501 catcacatga ttctccagca aataaaattg cgacttgtgt taaaggtaag ttttcatggg
     4561 gacatattaa ttcagatcaa cgattaacta aaccactagt aaggaaaaat ggtgagttcc
     4621 atgaagtaga atgggatgaa gctttaaacg tcattgcaga taattttaca gctattaaag
     4681 aaaagcatgg cccagatgca ctatcattca tttcttcttc taaagcgacg aatgaagaat
     4741 cgtatttaat gcaaaaatta gcaagacaag ttattggcac aaataacgtt gataactgtt
     4801 caagatattg ccaagcacct gcaacaaaag gcttatttag aacggttgga cacggcggtg
     4861 actcaggtag tattgaagat ttagaaaaag cggcaatgtc tgtattgata ggtactaata
     4921 cagccgaagc tcatccagtt atcgcatcac gcatgaaacg tgcacaaaaa ttatttggtc
     4981 aaaaaataca tgtatttgat attagaaaac atgaaatggc agaacgtgcg gatcgttttt
     5041 atcaacctaa accaggtacg gatttagcgt ggttaagtgc agtgactaag tatattattg
     5101 atcatgattt acacgataaa gcatttattg atgagtgggt agatgatttt gatgaatatt
     5161 acaaatcatt agaaacattt acaatggctt ttgctgaaga agcaacaggt attcctgaat
     5221 cagaattgat taaatttgct gaagaatgtg ctaaagctga atctgttgta atttgttggg
     5281 caatgggtat tacacaacaa gacattggta gtgactcaag tacagcgatt tcaaacttat
     5341 tattagtaac aggtaattat cgtcgtcctg gtactggtgc gtatccatta cgtggacata
     5401 ataatgttca aggatgtagt gatatgggaa gtatgcctga taagattact ggttatcaaa
     5461 gtattgaagc ggatgatatt cgcgctaaat ttgaaaaaga atatggcgtt aaattgaatc
     5521 caaaagctgg taaagataat catgaaatgg tagaaggtat acatgacgga gaagtacact
     5581 cattgtactt atatggtgaa gatacgggta ttgtggattc aaatattaat tttgtacaag
     5641 ctgcgtttga aaaattagat ttcatggtag tccaagatga atttttaaca ttcacagcaa
     5701 catacgcaga tgttgtattg ccagcaagtc cttcacttga aaaagacggt acttttacaa
     5761 ataccgaacg tcgtattcaa cgtttatacc aagcattaga acctcttggt gattcaaaac
     5821 ctgactggaa aattttccaa gcaattgcta atagattagg atttgattgg aattacaagc
     5881 atcctagtga aattatggat gaagtagcac gcttaacacc tctatatgct ggggtaagtt
     5941 atgatcgttt agaaggattc aatagtttac aatggccagt acaacctgat ggtactgatg
     6001 agcctattct atacttagaa ggattcaatt ttgataatgg taaagcaaaa ttattcccat
     6061 tatcatttga taattacttt aagcaagatg aaatttatga tattcatgta aacaatggtc
     6121 gattgttaga gcacttccat gaaggtaata tgacttatca aacaccaatg attaaataca
     6181 aagtgccacg tgcatttgtt gaaatttctc cagagcttgc tgaagataga ggcattcatg
     6241 aaggtgcaga agttaagctg atttctgaaa caggagaagc ggtattacaa gttcacgtga
     6301 cagatcgtgt taaaggtaaa gaaatctata ttccattaaa taatgatgcg atggaaaatg
     6361 gagatctagg tgcaattaac ttattaacta atagtgatgt tgatcaatac acggatacac
     6421 catcttataa acgtactagc tgtcgtttgg aggtaattac gaaacgtggt aagtcaccgt
     6481 tgaaccctaa taatttccgt gtcaataaaa aacgtcaacc gcagtacagt gttcaagtac
     6541 agaaaaaatg ggaacgttca gattatgttt tcccgggaaa tcaggtggat aaataatggc
     6601 tgagagaata tcttctaaaa ttagacgctt agaaaaatca gaagaacaaa ttaagttaga
     6661 aagtttaaat gaagttacag aggcaattgc agcgaataaa gatagtatat taaaagcaat
     6721 caagcttatt aaaacattag atgatgcaaa actattagat gctttaaatg gtgcaattcg
     6781 aggacgtcaa gtcatcataa ataaatttgc agttgaactt aataaagata tttatacagg
     6841 gctattatct aatatggctt caatggtatt tttattaggt gaactcaatg tatcagattt
     6901 aagcgacttc ctaaataaag ttaataaagg attacacgtt gccaatcaag cgagtccaaa
     6961 tgctaagaca actataagaa gtctattggg cgtattgaaa gatgacgaca tgaaccgtag
     7021 tttaacatac atgcttaata tgctaaaagg tatgtctcga gaagaataac attacaatga
     7081 aaaagtgttt ctacatgcaa gcagtgtagg agcacttttt ttaatttttg taaaaattaa
     7141 aaagggtgtt tttgatggaa taaatgaata atattgaaaa acaaaaaaat cacgtgctat
     7201 tgtgaatcaa tcatcgacag gcctatgaaa catcatgtat tattatgatt atgtaagtta
     7261 tttaaataaa aagggtgagt gcatattaat tcgagaatgt taaaaacgtc agctgtaatt
     7321 tctaatacat tattagtcat aggtttagtc tgtctattta tgatgaaatt agtgttagct
     7381 attacttttt ttgctgtttc attatcaatt agtctagttg tctttaatat gatgttcaga
     7441 gatagaacaa cgatgaaagt aatcgttaac gcttcttttt taatcgtaat attagcgatt
     7501 gtagtggcat attttttatt gtctaagtaa gaaaaatgag gttttgtcta tatgaagaag
     7561 aatttcaagt tacgcatttc aacgctacta ttgatagtta ttttagttgt ttttgctgta
     7621 ttactcatcg tgaatgaaac taaattgttt aaaaatgatg tgaattactc ttttgatgag
     7681 gctgtttcaa tgcaacaagg gaaaggtatt gtacagacaa aagaagagga tggtaaattt
     7741 gttgaagcaa ataataatga aattgctaaa gcaatgacta tttcacataa agacaatgat
     7801 atgaagtata tggatatcac agaaaaagtg ccaatgtcgg aatctgaagt taaccaattg
     7861 ctaaaaggta aggggatttt agaaaatcga gggaaagttt ttctagaagc tcaagaaaaa
     7921 tatgaggtta atgtcattta tcttgttagc catgcattag tagaaacagg taacggcaaa
     7981 tcagaattag caaaaggcat taaagatggg aaaaaacgct attacaactt ttttggtata
     8041 ggagcattcg atagtagtgc tgttcgtagt gggaaaagtt atgctgaaaa ggaacaatgg
     8101 acatcaccag ataaggcgat tattggtggt gcaaagttca ttcgtaatga atattttgaa
     8161 aacaatcaac tgaatttata tcaaatgcga tggaatccag aaaatcctgc gcaacatcaa
     8221 tatgcgagtg acattcgctg ggcagataaa attgccaaat taatggataa atcctataag
     8281 cagtttggta taaagaaaga tgatattaga caaacatatt ataaataaga catcggtgct
     8341 taaaggagct ggaacaattt attgtttcga gctcctttag cgcattctga gtgtgttagt
     8401 taaatggatt ttaacctaac aaaaaacgct atatagcatc aaatatgcta tatcccacat
     8461 cattgttaca aatgtacatg atgtaaatga atattgctgt ctaaatgtgc atgtaatata
     8521 caatggtgca gataatacac ttaagtcctt aaaaatgaaa cgttagttcc aagagtcatt
     8581 tttaaacaat agtgcatgtg ataaaataga aaagaatgaa aaatatagag gtgacaatat
     8641 gaagatagca attataggtg caggcatcgg tggattaaca gctgctgcat tattacaaga
     8701 acaaggtcat actattaaag tctttgaaaa aaatgagtca gttaaagaaa ttggcgctgg
     8761 gattggtatc ggagataatg tgcttaaaaa actaggtaat catgacttag ctaaaggtat
     8821 taaaaatgct gggcaaatct tatctacaat gacagtgtta gatgacaaag atcgcctgtt
     8881 aactactgtt aaattaaaaa gtaatacatt gaatgtgacg ttaccacgcc aaacattaat
     8941 tgacattatt aaatcttatg taaaagatga cgcaatattt acaaatcatg aagtcacgca
     9001 tatagataat gagacagata aagttaccat acatttcgcg gaacaagaaa gtgaagcatt
     9061 tgatttatgt attggtgctg atggaattca ttctaaagtg agacaatctg taaatgctga
     9121 cagtaaagta ttatatcaag ggtatacatg ctttagaggt ttaattgatg atattgattt
     9181 aaagcatcca gattgtgcaa aagaatactg gggaagaaaa ggaagagtag gtattgttcc
     9241 gttattaaat aatcaagcat attggttcat tacaattaac tcgaaggaaa acaatcataa
     9301 atatagttcg tttggtaaac ctcatttgca agcatacttt aatcactatc caaatgaagt
     9361 tagagagatc ttagacaaac aaagtgaaac aggtatctta ttgcataata tttatgattt
     9421 gaaaccactc aaatcttttg tttatggtcg tactatttta ctaggagatg cagcacatgc
     9481 gacaacgcct aatatggggc aaggtgctgg acaagcaatg gaagatgcta tcgtattagt
     9541 aaattgtttt aacgcatacg actttgaaaa agcattacag cgttatgata aaatacgtgt
     9601 caaacatact gcaaaagtaa ttaagcgttc tagaaaaatc ggtaaaattg cccaatatcg
     9661 tagtcgttta tttgttgcag ttagaaatcg tattatgaaa atgatgccaa atgcattagc
     9721 agctggacaa actaaattct tatataaatc gaaagaaaaa taatacaaca atatgaaaac
     9781 ccccgtatgt tgaaacgaga gctcaacata tgggggttct tgtttttata atgttattat
     9841 aataaattca attattagtt aacgacaaat tgtggtttct caccttgaac ggcactaatt
     9901 gcagcattag caacaatttt agacatcatg tcacgtgctt caaatgtagc attaccaata
     9961 tgcggtgtta atactacatt attaagtgat tttaagtcat cggtaatatc tggttcaaat
    10021 tcatatacat caagtgcagc accttcaatt tcattatctt tcaatgcttg cactagtgct
    10081 tgttcgtgca cgattggacc acgagaggca ttgattaaat acgccgtaga tttcatcatt
    10141 ttaaattgtt ctgtatcaat taaatgatgc attttaggat tataagcagc gttgatagtg
    10201 ataaaatctg cattctttaa tagtgtatct aaatctacat attttgcacc gatttctcgt
    10261 tctttttctt ctttgcgatt aggtccagtg tatagcacat ccatgtcaaa tgctcttgca
    10321 cgacgagcta ctgcactacc aatttcacct aaaccgataa tgccgattgt tttcccagat
    10381 acttctctac ctctgaaaaa taaaggtgcc catccatcaa atccagttgt acgtgataat
    10441 tggtcccctt caacaatacg acgcgctact gcaagtacta atccaattgt taaatcagca
    10501 gtcgcgtttg ttgatgcttt aggtgtgttt gtaacatcta tacttttttc tcgggcatac
    10561 tcgatatcaa tattattaaa accagcgcca tagttggcaa tgatttttaa gtctttacca
    10621 gcatcgataa catctttatc aacgtttgta gataataaac taattaaggc agtcgcgttt
    10681 ttaacacctt taattaaagt gtctttatcg actaatcctt taccttcata catttcaact
    10741 tcaaaatgtt cttgtaaaag ttttaaacct acttctggta ttgcaccagc aacataaact
    10801 ttttccataa aagatcactc cttttatctt agtatagtag aagattagac agtatacaac
    10861 tatgtcatga tgtcttgtgt atcaatgatg taagcgcgta cttttgatgg aggtgatata
    10921 acttaggcac tgtagaacta tgaatattgt aatgtggaaa aactggatca attaaattag
    10981 ataacgtagt tttaaagtta atagtattag aaaaaattaa tattttgaat atgggaggaa
    11041 atataaataa gtaggtggca acgaaaaata gcaaaaaaag agcttctcct ataaaggaaa
    11101 gctcaaagtt ttttgatgac atatgtacta gaattaagtt tcaagacaat atgtatcatc
    11161 gtgtttatat taaatatgga tgtagttgta gttacctgct tcacttgcag aaatagttct
    11221 agaacttact gagaaaggtc cgccactata attcatttct gaaattgtaa ctgaaccatc
    11281 actgtttaca ctttctacat atgcaacgtg accaaatggt ccttcagaag attgtaggat
    11341 agcgccttta gaaggtgtat gatttactgt gaatccagca ccttgtgcag cagcagccca
    11401 attattagca tttccccaag tagaaccgat ttctccgcca actttatcat atacatacca
    11461 agtacattgt cctgcagtgt ataagttacc agaatgtgaa attgatgatg tagttgtcgt
    11521 agtcgttgta gtttgagtcg tgttgtagtt atagttgttg taatttgtat aattttcagc
    11581 agcatctgca tgatgtgctt gacctactaa tgctgtgccg attcctgctg ttaacgtagt
    11641 tgctgttact aattttttca tgaataaagt cctccaaagt tctatatctt tttttataaa
    11701 taaaacgtag cgactgtttt attctcacat ctcgaattga tgacaatagt tactttaaca
    11761 aaattaatgc ttcttgtggg gaatgttatt gatttgtaaa agaataaaaa aactttgact
    11821 aattttgtaa taa
//
LOCUS       DAKTIP010000071        11566 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAKTIP010000071 DAKTIP010000000
VERSION     DAKTIP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.071"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            130..1242
                     /locus_tag="O0305_001414"
     CDS             130..1242
                     /locus_tag="O0305_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCY0274562.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            1264..1887
                     /locus_tag="O0305_001415"
     CDS             1264..1887
                     /locus_tag="O0305_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0274563.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            2243..2707
                     /locus_tag="O0305_001416"
     CDS             2243..2707
                     /locus_tag="O0305_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0274564.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            2886..4010
                     /locus_tag="O0305_001417"
     CDS             2886..4010
                     /locus_tag="O0305_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCY0274565.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLNQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            4079..4423
                     /locus_tag="O0305_001418"
     CDS             4079..4423
                     /locus_tag="O0305_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCY0274566.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            4806..4904
                     /locus_tag="O0305_001419"
     CDS             4806..4904
                     /locus_tag="O0305_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274567.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            5195..6391
                     /locus_tag="O0305_001420"
     CDS             5195..6391
                     /locus_tag="O0305_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0274568.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            6381..9317
                     /locus_tag="O0305_001421"
     CDS             6381..9317
                     /locus_tag="O0305_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0274569.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQSNIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            9314..10255
                     /gene="yhaM"
                     /locus_tag="O0305_001422"
     CDS             9314..10255
                     /gene="yhaM"
                     /locus_tag="O0305_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCY0274570.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(10376..11338)
                     /locus_tag="O0305_001423"
     CDS             complement(10376..11338)
                     /locus_tag="O0305_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCY0274571.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         4321 a         1628 c         1967 g         3650 t
ORIGIN      
        1 aaatcagttc tccgaaatat aactattgag taatcaaggc cattttatca tgtttacgtc
       61 taagatttaa aaatataata actcaatgta taatagttaa tacacgtaaa aatgaattgg
      121 agcgatttga tggaacaaag gacgcgacta gctttattaa aagagattgc cgaattttta
      181 aacgaagaaa ctgaaatgta tagcatgacc caaggtgcgt taaaatattt aattgagggc
      241 agtaatttca cgacaggctg gatatttttc atcaatagcg taggtgaaca tgaacttgta
      301 tcacatgtgg ctctaccaca atctttgaca gcagaccact gtcactatat taaagatggg
      361 tcttgttggt gtgtgaaagc atttaatcaa cgtaggctaa tgaaagcgtc gaatatcgtc
      421 aattgttctc gtattaactt agcctctaaa gcatttccta gccaaaatga caatattaca
      481 catcatgcaa cggtaccact taaatcgggt caagaacaat tcggcatttt aaatgtagcg
      541 tctccaaata ctgaaatata tagcgatgaa gatttagagt tgctagagtc tgtcgcattt
      601 caattagggt cagccatcaa acgtatttat ttaacagacc gtgaaaaaga agcggcaaaa
      661 attaatgaac gtaatcgttt agctagagat ttacatgatt cagtaaatca aatgttattt
      721 tctgtaaaac taacagcaca tgctgcgtat ggcatgtcaa acgaatccat cgctaaacaa
      781 gcattcaaga cgattgagga aacgagtcaa aatgccgtta atgaaatgcg tgcattgatt
      841 tggcaactta agccagttgg acttgagcaa ggtttaattc atgctttgac agcatatagt
      901 aaattaatgc atatacaatt aaatgtaaat gtagaaggtt taatcgattt atctaatgaa
      961 atcgaagaaa acatatacag agcattacaa gagtgtatta ataatgttaa gaaacatgct
     1021 gatacgaata aaatggatct tacattaaaa caaatgaacg atatactata tattgacgtg
     1081 attgattatg gtcaaggatt tgagatcgat aatgttcaga tagcttcatc acacggtatt
     1141 aataacataa aacaaagagt taaattatta agaggtaaag tgacctttca ttctcagcca
     1201 acaaagggta cacaaataca attcacaatt cctataaaat agcttgattt aaggagataa
     1261 cccatgaaca aagtaatatt agtagatgac cattatattg tgcgacaagg attgcgattt
     1321 ttattatcca cgattgaaaa catagaagtt ttacaagact ttgcagatgg agaaacattt
     1381 ttagaatatt taaaagagca tgagcaccct gatattgtgc tattagattt agtgatgcct
     1441 ggcatgaatg gtattgaaat tacggaatat attaaggcac attatccgga tattaaagtt
     1501 ttggtattaa caagttatgt tgatgatgaa catgtaattt cagcaatcaa taaaggtgct
     1561 gatggttatg aaatgaaaga cgttgagcct cagcaattaa ttgaaactat tagacgagtt
     1621 atgaacggtg aaaaaatgat acatcctaag gcacaagatg tattcgaaac agttagccaa
     1681 aaaccacact acacgaataa gttgtcaaag agagaaattg aagtgttacg tgaaatggtt
     1741 aaaggtaaaa caaataaaga gattgcagaa actttatttg tatctgaaaa aacaattaaa
     1801 acacatgtca gtcatatatt tagtaaatta caagttagcg atcgtacaca agcagcaatt
     1861 tatgcaatgg aaaataagtt gatttagaat gagcttttaa atatttgtcg aaaaatagac
     1921 aatttttaat ctgattttga tgaatttttt atagcgatag ggtttattat acagttttct
     1981 ttgtaaattt gatatcatta taaaagtgta agttttatca aatctagtga ttatgcataa
     2041 aaatttactt catttacttt cgcttcatta taaagtaaac ttaactcttg ttgacttgat
     2101 caaaatttaa tacaaatgtg aaattgactc ttttcatttt attaaaaata tattaagatg
     2161 taatctgtgt ttcaagaaat gtgtattatt gcaatttctt gtgtccggat aaaattcccc
     2221 aaacagaaag gtagataaca gaatggatag acagagtttt acagatttaa ttcaaacaaa
     2281 atttaaaatg gttcgtatag aggctggtta tacgcaagat actatggcgc aaacaattgg
     2341 actttctaaa aagactttag tacaaatcga aaaagagaga gtattaccaa actggacaac
     2401 ttgtatttca atttgtgcat tattcagaga ctctgaagta ttaaacagta catttggctg
     2461 tgatccatta gaaatcgttc aaacaatttc aagaaatcac tgtgcatatc caaaccatgc
     2521 accaacaagt gatatttatt ggaataacat tgagactcgt aatgggtaca ttttacaaag
     2581 taacaaagta agcaatattt atcgtgtatt aaatccagat aaccaaccaa ttttcggtac
     2641 ttctaaaatg agagaagctg aaacatactt taacagaaat gctaaagaag aattagtaca
     2701 tatctaggcc tatatacttt taataaataa taaaagcgat gtaacacacc tgtttcgaga
     2761 ggtgaggcat cgctttttgt atgtaaacta ataataaatt gagcagaaag tgaaatgatt
     2821 aatgaattaa aaaataaaaa tagtggtata atgaataact atgttgtcac aaaggagtca
     2881 aaattatgaa tgcactattt attatcatat ttatgattgt agtaggggcg ataattggtg
     2941 gcattactaa tgtaattgca attagaatgc tgtttcaccc ctttaaacca tactatatat
     3001 ttaaatttag agtcccattt acaccaggtt taataccgaa aagacgcgaa gaaattgcaa
     3061 ctaaaattgg ccaagtgatt gaagagcatt tgcttacaga aactttaatt aatgaaaaat
     3121 tgaaaagcga gcaatcacag caagcaatag aatctatgat tcaacagcag ttacaaaagt
     3181 tgacgaaaga tcaattgtca ataaaacaaa ttacttctca aatcgatatt gatttagaac
     3241 aagtattaca aactaatgga aatcaatata ttgaatcaca attgaataat tattatacaa
     3301 agcatcaaaa ccaaacaata gcatctttat tgccaaatca acttgtaaca tttttaaatc
     3361 agcatgtaga taacgcaaca gacttattat gtgatcgtgc aaggaattat ttatcatctg
     3421 caaaaggcac acaagatatt aatgatatgt tggatacttt tttcaatgag aaaggtaagt
     3481 taattggtat gttgcaaatg tttatgacaa aagagagtat tgcagatcgc attcaacaag
     3541 aacttatacg tttaacatct catcctaaag caagaacaat tgtgacatcg ttaattacca
     3601 atgaatatca aacttttaaa gataaaccat tgaatgaact gttagatgca tcgcagttta
     3661 atgaaatcgc tgaaaatcta tcagtgtatg taacaacata tgcaagcaaa caagcgaata
     3721 agccagtggt tacgttaatg cctcaattcg ttgattattt agaaggtcaa ctctctagca
     3781 aattagcaaa tttaatcatt gaaaaattat cgattcactt atctacaatt atgaaaaaag
     3841 tggatttacg aggattaatt gaagaacaaa ttaatacgtt tgacttagat tatattgaaa
     3901 aattaattat agaaattgct aacaaagaac taaagttgat tatgtcatta ggtttcattt
     3961 taggtggtat tattggattt ttccaaggtt tggttgcaat ctttgtctaa aaaagcgaga
     4021 tgtggtaaag taatatcgaa cgttttaata aacatcacaa caaaggagtg ctataaccat
     4081 ggcagtaaat ttatatgatt atgcaaatca attagaacaa gctttaagag aaagcgaaga
     4141 atacaaagca atcaaagaag cattcgctaa tgtaaaagct aacgaagaat ctaaaaagtt
     4201 attcgacgag ttccgtgaaa ctcaaattaa cttccaacaa aaacaaatgc aaggtgaaga
     4261 aattgctgaa gaagatttac aaaaagcgca agaacaagcg caagcaattg aaaaagatga
     4321 aaacatctct gcattaatga atgctgaaca aaaaatgagt caagtattcc aagaaatcaa
     4381 ccaaattatc gttaaaccat tagacgaaat ttacgctgac taatattaaa ataatatttt
     4441 tcaatccttt aaatagagga atagactgcc gacagagtcc gagactttgt gggtagtttt
     4501 tttagttttg ataacggaag ttagaggctc tctgtcaaat tgggcagatg cattcagtaa
     4561 cggaagttag gctagtaaac gtgtcctaac ttcctttttg cattttgaag tgtaaagttt
     4621 tcaacataat actattagtt cggtcatgta tcggactgat ggaaaagcgt ttcactttta
     4681 atgactcatt aagaacggcc tgaaaatgtt tggcgtatta agtgcaatga tagttttgac
     4741 atttagtttc taattggtca ttactgccga gcaaatctag tagagtaatc atgtaaatct
     4801 ttaatgtgcc atttgattca ctagcggtgt taataactac ggaaattgca tttccgactg
     4861 aaatttttga aaaatatcaa cgtacgctac aaataaaatt ttaaactgtt ataaatgtgt
     4921 ctcaatttca tatgttcatc gacgatatga agcgtattat ggtaaaatga agaaataata
     4981 aacttgttaa taaataaaac atcacgattt gactaaagca ctttattatt gtgtagataa
     5041 tagtttttta acgaaataaa aatggcgact ggttttaata aatcagctaa tgaatcacta
     5101 cacctataag tatgaatata gtgattagaa tgctttgtat agttggattt tgcaaaattg
     5161 atgttagaac ctttaattaa ggagatatgg atgtatggtt aaatttattc attgttcgga
     5221 tttacattta gatagtcctt tcaaatctaa gagtcatata agccctaaaa tttttgagga
     5281 tgttcaaaaa agtgcttatg aaagttttaa aaatattgta gatattgcat tacaacaaga
     5341 tgttgatttt gtaattatcg cgggtgattt atttgatagt gaaaacagaa cattacgtgc
     5401 tgaaattttc ttaaaacagc aatttgaacg tttacaaaat gaacaaatct ttgtttatgt
     5461 ttgtcatggt aatcacgatc ctttatcatc aaagatttca tcaaactggc cagataatgt
     5521 ttctgtattt tcaaataaag ttgagacgta tgaagcaatt actaaatctg gtgaaacaat
     5581 ttatattcac ggatttagtt atgaaaatag agcaagttat gagaacaaga ttgatgaata
     5641 tccatcaagt caaggccaaa aaggcataca tattggtgtc ttgcatggta cgtatagtaa
     5701 atcttcagtt aacgaaagat ataccgagtt cattttagaa gatttaaaca gtaaattgta
     5761 tcattattgg gctttaggtc atatacatga acgtcaacaa ttaagtgata tgcctgtaat
     5821 taactattca ggtaatattc aaggtagaca ttttaatgag caaggtgaaa aaggttgctt
     5881 attaatcgag ggtgaccact taaaattaaa gactaaattt tatcctacac agtatattag
     5941 atttgaagaa gcaactattg aaacggataa gacatctaag caaggtttat acgaggtcat
     6001 tcaaaacttt aaagaacaag tgagagaaga aggaaaagcc ttttatcgtt taacgcttgt
     6061 tattaatagt gagacattaa tttcacctca agatttatta caagttgaag aaatgattac
     6121 agattatgaa gaaaacgaaa atcaatttgt atatattgat gagttaaaaa tacaatatgc
     6181 acaaaatgat gagtcacctt tagttaatga attttcagcg gaattattag tcgatcaaac
     6241 tgtttttgat aaagcgatgt cagatttata tttaaatcca agggcatcta agttcctaga
     6301 cgattatgga acattcgacc atacagcatt agttaatcgt gctgaagaaa tattaaaagc
     6361 tgaaatgaga ggtgaacaaa atgataatta aatcacttga aatttatggt tacggtcaat
     6421 ttgttcaacg taaaattgaa tttaataaaa acttcactga aatttttggt gaaaatgaag
     6481 cgggtaaatc gacgattcaa gcattcatcc attcgatatt atttggattt ccaactaaaa
     6541 agtctaaaga gccaagacta gaaccacgtc taggtaacca atacggtggt aaattagtac
     6601 ttattcttga tgatggctta gagattgaag ttgaacgaat taaaggcagt gctcaaggtg
     6661 atgtgaaagt atatttacct aatggtgctg tgcgtgatga tgcttggtta caaaagaaac
     6721 ttaattatat ttctaaaaag acatatcaag gtatcttttc atttgatgta ctagggcttc
     6781 aagacattca tagaaatcta aatgaaaaac aattgcaaga ttatttatta caagcagggg
     6841 ctttaggatc aactgaattc acgtcaatgc gcgaagtgat taatcgtaaa aaagatgaat
     6901 tatataaaaa atcaggtaaa aatccgatca ttaatcaaca aattgagcaa ttaaaacaac
     6961 tagaaagtca aattcgtgaa gaagaagcaa agctagaaac atatcatcgc ttagtagatg
     7021 atcgagataa atcatcacgt cgattagaga atttaaagca taatttaaat caattatcaa
     7081 aaatgcatga agaaaaacaa aaagaggttg ctttacatga tcattcacaa gaatggaagt
     7141 ctctagaaca acagttaaat attgagccaa tcacattccc agaaaaaggt gtggatcgtt
     7201 acgaaaaagc acgagcgcat aagcaatcgt tagaaagaga tattggttta agaaatgagc
     7261 gtttagctca acttaaagaa gaagcgactc aattagagcc agttaaacaa tctgatattg
     7321 acgccttcat tagtttgaat caacaagaaa atgaaattaa aaataaagaa tttgaactta
     7381 ctgcaatcga aaaggatatt gcgaataaac aacgtgataa agatgaattg caatcaaata
     7441 ttggttggtc tgaaacgcat catgacgtag atagttcaga ggcaatgaaa agttatgtca
     7501 gtgagcaaat caagaataaa caagaacaag ctgcatacat taaacaatta gaacgtagtt
     7561 tagaagaaaa taaaatcgaa gataatgcgg ttcatagcga actagattct gttgaagaaa
     7621 aaatagttcc tgaagagact tttgaaaaga aaaaagaata ctcacaacaa gtcattgaat
     7681 taaacgaaaa agagaacttg tatagcaaat tgaaagaacg tttcgaaatt gagcaacaag
     7741 aaaaacaaaa acgtcaaaaa ttgttacgaa caacgtttat tcttttaaca ttagtaggta
     7801 ttggtttaac tgccttctca tttatttcaa acaacatgtt gtttggtatt attttcgcag
     7861 tattaacttt agtatttgtt atcggtatca tcatgtctaa atcaaaagaa gtagattata
     7921 gcgaggccat cacagatgaa atcgaagaaa ttaaagcaca actagccatt ttagatgaaa
     7981 attatgactt agattttgat cttgatgagc aatatcgcat tcgtgatcac tggcaacaag
     8041 ctttgaaaaa taaagatatt ttagaagaga aacgtcagta tattgaaggt cgtttaaatg
     8101 atgcgaaagg gcgtcatgat gaattacaat cgacagttga gaacgttaag gatgaattat
     8161 acttatcatc taaaatttca aatgatttaa ttgttgatag tatttctaca atggcgaata
     8221 ttaaagcatt agatcaacat atcagtgatt taaatcaaca acgccagcaa ttggttcaag
     8281 aattagatac attctacaat catgctgaag ctgttacaaa atcacaattt gtttatttca
     8341 acaaattatc tttattccac gatgtgcaac aatggttgaa atcagctgaa gacacgaatg
     8401 aaaaatggcg tattaatgct gaaaatacca aacttgttac aaatgaatta aatcatttaa
     8461 atgctcaatt agaagaaaat aataaagaaa ttacagcact attcgatttt atcaatgttg
     8521 gtactgaaga agatttttac caacatcatg aagattacca aacttatact agtaatttga
     8581 gtcgttttaa tgatttaact aaatatcttg agaaccaaaa ctattcttat gaattaagtt
     8641 ctagtttaag tgaaaaaaca actgcacaac ttgaagaaga agatcattta ttggctactc
     8701 aagttgacga atataatgag caatatcttg aaatgcaagc acaagtcagt gatttaagtg
     8761 cacaaatcaa tcacatggaa actgatacaa cgcttgctaa tttaagacat gaatatcaca
     8821 gtcttaaaaa tcaacttaat gatatcgcaa aagattgggc aagtttaagt tatttacaaa
     8881 gtttagttga tgaacacatt aaacaaatta aagataaacg tttgcctcaa gttattaatg
     8941 aagcggtaga aatattgaag catttaacag atggcagata tacgatgatt aactataatg
     9001 aagattcaat tacggttaaa catgttaatg gtcaattata tgatcctgtt gaactaagtc
     9061 aatctacaaa agaattactt tatgtagctt tacgtatcag tttaattaag gtactaagac
     9121 catattatcc gttcccatta attgttgatg atgcatttgt tcattttgat aaaaaacgta
     9181 ctgaaaaaat gttgaattat ttacgatcat tatcagaaca ctatcaagta ctatacttta
     9241 catgtgtaaa agataatatt gttccatcaa aagaagtgat tacattaaac aaaatagagg
     9301 aaggcgggaa acgatgagaa atatagagaa tctaaatccc ggagattcag ttgatcactt
     9361 tttcttagtg cataaagcta cacagggtgt aacagcacaa ggtaaagatt atatgacatt
     9421 acatttgcaa gataaaagtg gtgaaattga agcgaaattt tggacggcta caaaaaatga
     9481 tatggcaaca atcaagcctg aagaaattgt acatgttaaa ggtgacatca taaactatcg
     9541 cggaaataaa cagatgaaag tcaaccaaat tagactagcg acaactgaag atcaattaaa
     9601 aacagaacaa tttgtagatg gtgcaccttt atcaccggca gaaatacaag aagagatttc
     9661 tcattatttg ctagatattg aaaatgctaa tttacaacgt atcacacgtc atttattgaa
     9721 aaaatatcaa gaacgatttt acacatatcc agctgctagt tctcatcatc ataactttgc
     9781 gagtggctta agctatcatg tattaacgat gttacgtatt gcaaaatcaa tttgtgacat
     9841 ttatccattg ttaaacaaaa gtttgttata tagtggtatt attttgcatg atattggtaa
     9901 agttagagaa ttgagtggtc ctgttgcgac gtcgtataca gtcgaaggta acttattagg
     9961 acacatctcg attgcgagtg atgaagtagt tgaagcagct cgtgaattga acattgaagg
    10021 agaagaaatc atgttgttaa gacatatgat tttatctcat catggtaagt tagagtatgg
    10081 ttctccaaaa ctgccatact taaaagaagc agaaatttta tgctatatcg ataatatcga
    10141 tgctagaatg aatatgtttg aaaaggcata taaaaaaact gacaagggtc agtttacaga
    10201 taaaatattt ggtcttgaaa atcgtagatt ctacaatcct gaatcactcg attaagaatt
    10261 aaaagatatc ggacagatga gtatactgaa acatgagctc atctgtccga tttttaatgc
    10321 gaaaaaaccg agcggccgtg gtatgatttt tgaaccacgg tcgctcggtt ttgtgttatt
    10381 ggctcatgcc ggattgtccg ccttgtgcgc caccttgttt aagtttttca gggtttaaga
    10441 ttttatcttc gacaactgat ttaatatcac gatctttaaa gtcaacatcg tattctttta
    10501 atagatcttt gtatgcatca gtcaataatt ttggattttt ttgtactttc tgatcgacta
    10561 atttttcttt caggctttgt ttttcactgt taaagtctgt tggtttatca gctttaataa
    10621 tatgatatcc aaagcttgat ttaacaacct ctgatacttc accatcttta agcttaaata
    10681 gtgctttttc aaaatcttta tcagtttgtc ctttaagaac ataacctaat tcgccatctt
    10741 ttttagctga accagtatcc attgattctt ttttagcgat ttcaccaaat ttacttggat
    10801 cttttgaaac ttctttttga atttcttcag ctttttgttt cgcttcttta tcatctaagc
    10861 cttctttgtc gcttttctta gatttaactt taattaaaat gtgtgaagct ttcttgctgt
    10921 cttctttaat ttcagaatca gagattttaa ttttatctga tagtaattct ttatgataag
    10981 cagcagtacg taaattttct ttatatttat cggctgttaa accttgctgt tgaagggcct
    11041 tttcaaattt atctttaccg ccgtattgct tttgcatttt ttcaatttgt tcgtcaatct
    11101 tcttatcatt aactttattt ttatatttat cagctaaaat tttatttaac atttcagtaa
    11161 atgatgcatt tgcaatttga tctttaccga tttttttcat tgtatctgca actgttacgt
    11221 ctccagcttt agaagaaatt aatgtatttt ctttagagtc tgtggcacta gcgccacaag
    11281 cgcctaataa taaagcacta gctgttaccg gaacgattaa tttgtttatc atcttcatag
    11341 ttgaaactcc tttgtaagtt aatcatcgtg aatataacat agttttatat tatcaccaat
    11401 caataaccca tgacatcaga ctagagcatt aggtttaggg gtagaaattg atgaaaataa
    11461 aaaaacaagg tctatataca tttgattgta agctattaca tagcctaaaa acaaataata
    11521 acaccttgct ataattattt tgttataaat gatatggatt tttaat
//
LOCUS       DAKTIP010000072        11548 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAKTIP010000072 DAKTIP010000000
VERSION     DAKTIP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.072"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            32..2737
                     /gene="acnA"
                     /locus_tag="O0305_001424"
     CDS             32..2737
                     /gene="acnA"
                     /locus_tag="O0305_001424"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCY0274572.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2917..3384
                     /locus_tag="O0305_001425"
     CDS             2917..3384
                     /locus_tag="O0305_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0274573.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(3591..3887)
                     /locus_tag="O0305_001426"
     CDS             complement(3591..3887)
                     /locus_tag="O0305_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCY0274574.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4263..4871)
                     /gene="plsY"
                     /locus_tag="O0305_001427"
     CDS             complement(4263..4871)
                     /gene="plsY"
                     /locus_tag="O0305_001427"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCY0274575.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLQVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5077..7068
                     /gene="parE"
                     /locus_tag="O0305_001428"
     CDS             5077..7068
                     /gene="parE"
                     /locus_tag="O0305_001428"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCY0274576.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7068..9470
                     /gene="parC"
                     /locus_tag="O0305_001429"
     CDS             7068..9470
                     /gene="parC"
                     /locus_tag="O0305_001429"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCY0274577.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVYEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            9720..11180
                     /locus_tag="O0305_001430"
     CDS             9720..11180
                     /locus_tag="O0305_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCY0274578.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4022 a         1653 c         2215 g         3658 t
ORIGIN      
        1 aatataaaaa tgtatcaagg gggatcatta aatggctgca aattttaaag agcaatcaaa
       61 aaaacatttt gacttgaatg gccaaagtta tacttactat gatttaaaag ctgtagaaga
      121 gcaaggtatt actaaagttt ccaatttacc ttattcaatt cgtgttttgt tagaatcttt
      181 acttcgtcaa gaagatgatt ttgtaattac agacgatcat attaaagctt taagtcagtt
      241 tggaaaagat ggaaatgaag gcgaggtgcc atttaaacct tctcgtgtta ttttacaaga
      301 tttcacaggt gtaccagccg tagttgattt agcttcttta cgtaaagcaa tggatgacgt
      361 tgggggagat attactaaaa ttaatccaga agtaccggtg gatttagtta ttgaccactc
      421 agttcaagtg gatagctatg caaatccaga agctcttgaa cgtaatatga aattagaatt
      481 tgaacgtaac tatgaacgtt atcagttttt aaattgggca acgaaagcat ttgataatta
      541 caatgcagtt cctcctgcaa ctggaatagt tcaccaagtt aacttagaat atttagcaag
      601 tgttgtacat gttcgtgatg tagatggtga aaaaactgca tttccagata cattagttgg
      661 tactgattca catacaacaa tgataaatgg tattggcgta ctaggatggg gtgttggtgg
      721 tattgaagct gaagctggaa tgcttggaca accttcttat ttcccaattc cagaggttat
      781 tggtgtacga ctagtaaatt cattaccaca aggcgcaaca gcaactgatt tagcgttaag
      841 agtaactcaa gagctacgta aaaaaggtgt tgttggtaaa tttgtggagt tctttggtcc
      901 aggtgtacaa catttaccac tagcagaccg tgctacaatt gcaaacatgg caccagagta
      961 tggagcaact tgcggattct tcccagttga tgatgaatct cttaaatata tgaagttaac
     1021 tggtagatca gacgaacata tcgcgctagt aaaagaatat ttgaaacaaa accatatgtt
     1081 ctttgatgtt gagaaagaag atcctaatta tacagatgtt atcgaattgg atttatcaac
     1141 agttgaagca tcgctttcag gaccaaaacg tcctcaagat ttaattttct taagtgatat
     1201 gaaatcatca tttgaaaatt ctgtaacagc tccagcaggc aaccaaggac acggtttaga
     1261 taaaagtgaa tttgataaga aagctgaaat taactttaaa gatggatcaa aagctacaat
     1321 gaaaacaggt gatattgcaa tagcagcaat tacatcatgt acaaatacat ctaaccctta
     1381 tgtaatgtta ggtgcaggtt tagttgctaa aaaagcagtt gaaaaaggct tgaaagttcc
     1441 tgaatacgtt aaaacttctc tagcaccagg atcaaaagtt gttaccggat atttaagaga
     1501 tgctggctta caaccttatt tagatgattt aggcttcaac ttggttggtt atggatgtac
     1561 aacttgtatc ggtaattcag gtcctttatt accagaaatt gaaaaagcga ttgctgatga
     1621 ggacctatta gtgacatctg tattatctgg taaccgtaac tttgaaggtc gtatccatcc
     1681 tcttgttaaa gccaattacc tagcttcacc acagttagtt gttgcttatg cattagctgg
     1741 aacggttgat attgatttac aaaatgaacc tattggtaaa ggtaatgacg gtgaagatgt
     1801 atatttgaaa gatatttggc catcaattaa agaagtttca gataccgttg atagtgttgt
     1861 aacacctgaa ttatttattg aagaatataa taacgtatac aataacaacg aattatggaa
     1921 tgagattgat gtaactgatc aacctctata tgactttgat cctaattcaa catacattca
     1981 aaatccatca ttcttccaag gattatctaa agaaccgggt acgattgttc cattaaatgg
     2041 tttacgtgtt atgggtaaat tcggtgattc tgtgacaact gaccacatct ctccagcagg
     2101 tgcaattggt aaagatacgc cagctggtaa atatttacaa gatcatcaag tgcctattcg
     2161 tgaatttaat tcatatggtt caagacgtgg taatcacgaa gtaatggttc gaggtacgtt
     2221 tgctaatata cgtattaaaa accaattagc gccaggtact gaaggtggtt ttacaactta
     2281 ttggccaaca aatgaagtaa tgcctatctt tgatgctgca atgaaatata aagaagatgg
     2341 tacaggttta gttgtattag ctggtaacga ttatggtatg ggttcatctc gtgactgggc
     2401 agcaaaaggt acaaacttat taggtgttaa aacagttatt gcacaaagtt atgaacgtat
     2461 ccatcgttca aatttagtta tgatgggtgt attaccatta gagtttaaaa aaggtgaatc
     2521 agctgattct cttggtctag atggtacaga agaaatttct gttaatattg atgaaaatgt
     2581 tcaaccacat gactacgtca aagttactgc taagaagcaa gatggtgatt tggtagaatt
     2641 tgacgctatg gttcgttttg actcacttgt tgaaatggat tactatcgtc acggtggaat
     2701 tttacaaatg gttttaagaa ataaattagc gcaataaaaa atagatatca cagtaaaatt
     2761 ttaatcggta tttgaggcac tttgatagtg ctcaaaaata cttctataaa ctttatgata
     2821 taataggtcg gttcaaaacc tttgtgttct tggccggcct cttcttgtat tttatgttag
     2881 aatgagatat aaagttaaac tgaaagaagg cttaaaatga tatatagtat tacagaaata
     2941 gaagcgcgtt atgctgaaac tgataagatg ggtgtaattt atcacgggaa ttatgcaact
     3001 tggtttgaag ttgcgcggtt ggattatata tcgaagttag gttttagtta tgctgatatg
     3061 gaaaaacaag gaatcatttc acctgtgact gacctcaatg tcaattataa aaagtctatt
     3121 ttttatccag aaaaagttaa agttaaaact tgggttgaaa aatattcgag attacgttca
     3181 gtgtataaat atgagatttt taatgaaaaa ggtgaacttg caactacagg ttccacagaa
     3241 ttgatttgca ttaaagaaga tacttttaag cctatacggt tggatcgtta tttcccagat
     3301 tggcatgaag cttatagtaa agtgcaaacg ctcaataatg aagggaaaac agtagagata
     3361 atggatggta ttgattcttt ataagttact gtaaatgttt ggagaggcaa tattaacaat
     3421 taatctataa aaaatagcca atacgttaac attaatgtaa ttagcgtgaa gataagctaa
     3481 tatatagcaa aaacgaactt gttcatacaa catcagatgt gctaaaccaa agtacaactt
     3541 ccgaggttca ggacacatca gaatgtgtaa acaagttcgt tttattttag ttattttgtg
     3601 gaataagaaa tttcatcttc gtgatcaact acatttacaa taatgtggtc atcttcaaag
     3661 taccacaaat ctttttccgc tacgacaaca tttaaatcgt catattgttg ttcatagcca
     3721 atatcaacat cttcctttgg ttcaactgta aaagcaggac taaatccttg cttgagttgg
     3781 aattcgccac catatcttac aaaaaacacg agcactttat tattttcagg caactcaagt
     3841 tcatttttaa accaagttac tgctgcatca gtaagttcta tttgcataac aatgacctcc
     3901 tatatttatt acagaatggg agtgggccaa cttgtattat ttgtggaatt tcttatcgaa
     3961 attctctgtg ttggagccca ccccaacctg ctcattattg taagctgaat ttccataagc
     4021 ttctatgttg ggacccccga ccagaattga aaaaacctag ttataagcgc attttgttcg
     4081 gttaactaca gctaatataa cattgtggag cctatgacat tgatttatgt ctcggtctca
     4141 aatgttcttg taaaaaaact aagtatagtt gaattttaag ttaaaaaaat tgtttttgca
     4201 aaaataaaat aatccatcaa ctgcaaaaaa atatgcaatg atggactaat gtcaaaaaat
     4261 tgttacatcc attttatttt aggttcttcg cctctaaaaa tccttgcgat attagagcga
     4321 tgtctaatta tcaatatgat tgaaactaag aaactaacga ctaataaaat atagtcttga
     4381 atgataagcg agccaatcac acagcaaatt gctgcaacga tacttgctaa agaaacatat
     4441 ttaaaaatct tcaatacaat aaagaagata attgcaagta ttagtaaaag tatcggattg
     4501 actcccaaga cgacacctgc actagttgca acagctttgc caccttggaa ttttaaataa
     4561 acaggataaa cgtgtccaag tatagcgaat aagccaacaa ttaaaccatt tgtaaaaaaa
     4621 gtactaatag ggccatctgc gtgaacttgt aaccataaag ggaagaaaac agttatgaac
     4681 cctttgaaaa tatctagaaa tgttaccaag aatcctgcag gacgacctaa tactctaaag
     4741 ctattagtag cgccagtatt accactacca aattgtctaa tatctttttt gaaaaataat
     4801 tttccaatta cgaatccact tgggaaagcg ccgataagat aacttagtag taacatgacg
     4861 attatcatca taaatattac acatccttta atatcttagg actattttat catattcatt
     4921 taaattacgg ctaaaaattt taaaaacggg gattaatata tggaattaag ctatgaaagt
     4981 taattgatac ttgcatttta cgctgattta tataagaata actattgtat agttttaaaa
     5041 acgaacgtac gtttgcagga ggcgaaatca ttggcaatga ataaacaaaa taattattca
     5101 gatgattcaa tacaggtttt agaggggtta gaagcagttc gtaaaagacc tggtatgtat
     5161 attggatcaa ctgataaacg gggattacat catctagtat atgaaattgt cgataactcc
     5221 gtcgatgaag tattgaatgg ttacggtaac gaaatagatg taacaattaa taaagatggt
     5281 agtatttcta tagaagataa tggacgtggt atgccaacag gtatacataa atcaggtaaa
     5341 ccgacagtcg aagttatctt tactgtttta catgcaggag gtaaatttgg acaaggcggc
     5401 tataaaactt caggtggtct tcacggtgtt ggtgcttcag ttgtaaatgc attgagtgaa
     5461 tggcttgaag ttgaaatcca tcgagatggt aatatatatc atcaaagttt taaaaacggt
     5521 ggttcgccat cttctggttt agtgaaaaaa ggtaaaacta agaaaacagg taccaaagta
     5581 acatttaaac ctgatgacac aatttttaaa gcatctacat catttaattt tgatgtttta
     5641 agtgaacgac tacaagagtc tgcgttctta ttgaaaaatt taaaaataac gcttaatgat
     5701 ttacgcagtg gtaaagagcg tcaagagcat taccattatg aagaaggaat caaagagttt
     5761 gttagttatg tcaatgaagg aaaagaagtt ttgcatgacg tggctacatt ttcaggtgaa
     5821 gcaaatggta tagaggtaga cgtagctttc caatataatg atcaatattc agaaagtatt
     5881 ttaagttttg taaataatgt acgtactaaa gatggtggta cacatgaagt tggttttaaa
     5941 acagcaatga cacgtgtatt taatgattat gcacgtcgta ttaatgaact taaaacaaaa
     6001 gataaaaact tagatggtaa tgatattcgt gaaggtttaa cagctgttgt gtctgttcgt
     6061 attccagaag aattattgca atttgaagga caaacgaaat ctaaattggg tacttctgaa
     6121 gctagaagtg ctgttgattc agttgttgca gacaaattgc cattctattt agaagaaaaa
     6181 ggacaattgt ctaaatcact tgtgaaaaaa gcgattaaag cacaacaagc aagggaagct
     6241 gcacgtaaag ctcgtgaaga tgctcgttca ggtaagaaaa acaagcgtaa agacactttg
     6301 ctatctggta aattaacacc tgcacaaagt aaaaacactg aaaaaaatga attgtattta
     6361 gtcgaaggtg attctgcggg aggttcagca aaacttggac gagaccgcaa attccaagcg
     6421 atattaccat tacgtggtaa ggtaattaat acagagaaag cacgtctaga agatattttt
     6481 aaaaatgaag aaattaatac aattatccac acaatcgggg caggcgttgg tactgacttt
     6541 aaaattgaag atagtaatta taatcgtgta attattatga ctgatgctga tactgatggt
     6601 gcgcatattc aagtgctatt gttaacattc ttcttcaaat atatgaaacc gcttgttcaa
     6661 gcaggtcgtg tatttattgc tttacctcca ctttataaat tggaaaaagg taaaggcaaa
     6721 acaaagcgag ttgaatacgc ttggacagac gaagagctta ataaattgca aaaagaactt
     6781 ggtaaaggct tcacgttaca acgttacaaa ggtttgggtg aaatgaaccc tgaacaatta
     6841 tgggaaacga cgatgaaccc agaaacacga actttaattc gtgtacaagt tgaagatgaa
     6901 gtgcgttcat ctaaacgtgt aacaacatta atgggtgaca aagtacaacc tagacgtgaa
     6961 tggattgaaa agcatgttga gtttggtatg caagaggacc aaagtatttt agataattct
     7021 gaagtacaag tgcttgaaaa tgatcaattt gatgaggagg aaatctagtg agtgaaataa
     7081 ttcaagattt atcacttgaa gatgttttag gtgatcgctt tggaagatat agtaaatata
     7141 ttattcaaga gcgtgcattg ccagatgttc gtgatggttt aaaaccagta caacgtcgta
     7201 ttttatatgc aatgtattca agtggtaata cacacgataa aaatttccgt aaaagtgcga
     7261 aaacagtcgg tgatgttatt ggtcaatatc atccacatgg agactcctca gtgtacgaag
     7321 caatggtccg tttaagtcaa gactggaagt tacgacatgt cttaatagaa atgcatggta
     7381 ataatggtag tatcgataat gatccgccag cggcaatgcg ttacactgaa gctaagttaa
     7441 gcttactagc tgaagagtta ttacgtgata ttaataaaga gacagtttct ttcattccaa
     7501 actatgatga tacgacactc gaaccaatgg tattgccatc aagatttcct aacttactag
     7561 tgaatggttc tacaggtata tctgcaggtt acgcgacaga tataccacca cataatttag
     7621 ctgaagtgat tcaagcaaca cttaaatata ttgataatcc ggatattaca gtcaatcaat
     7681 taatgaaata tattaaaggt cctgattttc caactggtgg tattattcaa ggtattgatg
     7741 gtattaaaaa agcttatgaa tcaggtaaag gtagaattat agttcgttct aaagttgaag
     7801 aagaaacttt acgcaatgga cgtaaacagt taattattac tgaaattcca tatgaagtga
     7861 acaaaagtag cttagtaaaa cgtatcgatg aattacgtgc tgacaaaaaa gtcgatggta
     7921 tcgttgaagt acgtgatgaa actgatagaa ctggtttacg aatagcaatt gaattgaaaa
     7981 aagatgtgaa cagtgaatca atcaaaaatt atctttataa aaactctgat ttacagattt
     8041 catataattt caacatggtc gctattagtg atggtcgtcc aaaattgatg ggtattcgtc
     8101 aaattataga tagttatttg aatcaccaaa ttgaggttgt tgcaaataga acgaagtttg
     8161 aattagataa tgcagaaaaa cgtatgcata tcgttgaagg tttgattaaa gcgttgtcaa
     8221 ttttagataa agtaatcgaa ttgattcgta gctctaaaaa caagcgtgac gctaaagaaa
     8281 accttatcga agtatacgag ttcacagaag aacaggctga agcaattgta atgttacagt
     8341 tatatcgttt aacaaatact gacatagttg cgcttgaagg tgaacataaa gaacttgaag
     8401 cattaatcaa acaattacgt catattcttg ataaccatga tgcattattg aatgtcataa
     8461 aagaagaatt gaatgaaatt aaaaagaaat tcaaatctga acgactgtct ttaattgaag
     8521 cagaaattga agaaattaaa attgacaaag aagttatggt gcctagtgaa gaagttattt
     8581 taagtatgac acgtcatgga tatattaaac gtacttctat tcgtagcttt aatgctagcg
     8641 gtgttgaaga tattggttta aaagatggtg acagtttact taaacatcaa gaagtaaata
     8701 cgcaagatac cgtactagta tttacaaata aaggtcgtta tctatttata ccggttcata
     8761 aattagcaga tattcgttgg aaagaattgg gacaacatgt atcacaaata gttcctatcg
     8821 aagaagatga agtggttatt aatgtcttta atgaaaagga ctttaataca gatgcatttt
     8881 atgtttttgc gactcaaaat ggcatgatta agaaaagtac agtgcctcta tttaaaacaa
     8941 cgcgttttaa taaaccttta attgctacta aagttaaaga aaatgatgat ttgattagtg
     9001 ttatgcgctt tgaaaaagat caattaatta ccgtcattac taataaaggt atgtcattaa
     9061 cgtataatac aagtgaacta tcagataccg gattaagggc agctggtgtt aaatcaataa
     9121 atcttaaagc tgaagatttc gttgttatga cagaaggtgt ttctgaaaat gatactatat
     9181 tgatggccac acaacgcggc tcgttaaaac gtattagttt taaaatctta caagttgcta
     9241 aaagagcaca acgtggaata actttattaa aagaattaaa gaaaaatcca catcgtattg
     9301 tagctgcaca tgtagtgaca ggtgaacata gtcaatatac attatattca aaatcaaatg
     9361 aagaacatgg tttaattaat gatattcata aatctgaaca atatacaaat ggctcattca
     9421 ttgtagatac agatgatttt ggtgaagtaa tagacatgta tattagctaa aaactatatg
     9481 caatcacgaa attaaatgat aaaatacagt aatgttaaat tttgactaaa ttcaagggat
     9541 ttatattaaa tgctgaccaa gtacttatcg ttaaattagc gatatacttt gaccaaacta
     9601 atataaatct cttgtattgt ataagaaatg atgatgtatc tctatattta ctgaaggttc
     9661 aattcggtct aattttatag aaggtacatg attcatgtag aggatgcgag gggtttattt
     9721 tgaaagattt cgatagttta atacctggat ggtttaaaga atttgtccat gttggtaccg
     9781 atttaatatg gtctcaatat ttaattggtc tattattgac agctggattc ttctttacga
     9841 ttagttctaa attcgttcaa ttacgaatgt tacctgaaat gtttagagct ttagtagaac
     9901 gtccagaaac tttagaagat ggtaagaagg gtatttcgcc attccaagca tttgcgatta
     9961 gtgctggttc gagagttggt actggtaata ttgctggtgt tgcgactgcg attgttttag
    10021 gcggtccagg tgcagtattt tggatgtggg ttattgcatt tataggtgca gcgagtgcat
    10081 ttatagaagc gactttggct caggtttata aagtacatga taaagatggt ggattccgtg
    10141 gtggtccagc ttactatatt actaaaggtt taaatcaaaa atggctaggt atcgtatttg
    10201 cgattttaat tacaattaca tttgcatttg tatttaacac agtgcaatct aatacaattg
    10261 cggagtcgtt aaatacgcaa tataatatta gtccagtaat cacaggtatt attttagcaa
    10321 tcgtaacagc tattattata tttggtggtg tacgtagtat tgctacgtta tcttcgttaa
    10381 ttgtaccgat tatggctatc atttacattg gtatggtttt agtaatattg ctatttaatt
    10441 tagatcaaat tgttcctatg ataggtacga ttattaaaag tgcatttggt atcgaacaag
    10501 taactggtgg cgctgtaggt gctgcggttc ttcaaggtat caaacgtggt ttattctcta
    10561 acgaagctgg tatgggttct gcgccgaatg cagcggcaac tgctgccgta ccacaccctg
    10621 ttaagcaagg tttaatccaa tcattaggtg tgttctttga tacaatgttg gtttgtacag
    10681 caactgcaat catgatttta ctatattcag gactgaaatt tggtgataac gcacctcaag
    10741 gtgttgcagt tactcaatca gcacttaatg agcatttagg ttctgctgga ggtattttct
    10801 taacaatagc agttacactg tttgcatttt catctgttgt aggtaattac tattacggtc
    10861 aatctaatat tgaattttta tcaacaaacc gtgtaatatt atttatcttt agatgtcttg
    10921 ttgtagtact tgtctttgtc ggtgcagttg taaaaacaga aacagtatgg aatacggcag
    10981 acttatttat gggcttaatg gcaattgtaa acattatttc cattatagga ctgtccaatg
    11041 tagcttttgc attgatgaaa gattatcaaa agcagaaaaa agaaggcaag aaccctgtct
    11101 ttaaacctga aaacttagaa attaacttat ttggaattag tgcttggggc gctaacaaat
    11161 ataagaactc tgataaataa atatttttaa tgtaacgatt cattatataa aaatcaaata
    11221 aaccctgagg ttttcaaatt aaagcgaaaa tctcagggtt ttaaatttgt actcaattat
    11281 catctgtatc attacactag ttaatctaag atttgattat atattaatat agtaaggttg
    11341 agatttattt cctccattgg tgtgcaaaga ctataccatt gataagacat attgttggat
    11401 agatgaatat aaatttgttt tagctttgca caatttaata gtacgtttta gtttgtattg
    11461 agtattaatt cagataatat ataaataaat agaattattt gtaaatatgg gagtcagatt
    11521 tttttattat agttattcca ctttgaga
//
LOCUS       DAKTIP010000073        11365 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAKTIP010000073 DAKTIP010000000
VERSION     DAKTIP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.073"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(424..1764)
                     /gene="glnA"
                     /locus_tag="O0305_001431"
     CDS             complement(424..1764)
                     /gene="glnA"
                     /locus_tag="O0305_001431"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCY0274579.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1783..2151)
                     /locus_tag="O0305_001432"
     CDS             complement(1783..2151)
                     /locus_tag="O0305_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0274580.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2394..3632)
                     /locus_tag="O0305_001433"
     CDS             complement(2394..3632)
                     /locus_tag="O0305_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0274581.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(3651..4889)
                     /gene="hflX"
                     /locus_tag="O0305_001434"
     CDS             complement(3651..4889)
                     /gene="hflX"
                     /locus_tag="O0305_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCY0274582.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            5001..5477
                     /locus_tag="O0305_001435"
     CDS             5001..5477
                     /locus_tag="O0305_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0274583.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            complement(5699..5932)
                     /gene="hfq"
                     /locus_tag="O0305_001436"
     CDS             complement(5699..5932)
                     /gene="hfq"
                     /locus_tag="O0305_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCY0274584.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(5947..6882)
                     /gene="miaA"
                     /locus_tag="O0305_001437"
     CDS             complement(5947..6882)
                     /gene="miaA"
                     /locus_tag="O0305_001437"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCY0274585.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(6900..7814)
                     /locus_tag="O0305_001438"
     CDS             complement(6900..7814)
                     /locus_tag="O0305_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274586.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(7964..9637)
                     /locus_tag="O0305_001439"
     CDS             complement(7964..9637)
                     /locus_tag="O0305_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCY0274587.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(9795..11291)
                     /gene="glpK"
                     /locus_tag="O0305_001440"
     CDS             complement(9795..11291)
                     /gene="glpK"
                     /locus_tag="O0305_001440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCY0274588.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
BASE COUNT         3461 a         2194 c         1587 g         4123 t
ORIGIN      
        1 atcgataaac aaaaagatat tttttgtttt gtttctcttt tcatatagta ttaccccctt
       61 aataatgcgt agtaaggtcc ctcttttcgg ggtcttacct tagaacgttc tgcaaatgaa
      121 ttcgatgaga agtaatatga atatggctat tttcaagtaa tactcaacgt tttcgcgacg
      181 ttcttttatc gcctcatctc atcacctcca aatatattaa aattcatgtg aactaaaata
      241 taaaatggtc ttccccagct ttaaaaaaat aaatacataa aacatttact tgaccaaaac
      301 ttgaccaaat ccatttttta acaaaagtgg tcaagtgaaa tcccatcttt ccaaatcaaa
      361 aaagagctaa agcaaaatgc tctagccctt gatattactg atttcccatc attgtttagc
      421 gtattaatat tgcttcatgt actgatctct ttcccattca gagacttgag ttctgtagta
      481 atcccattca attgattttg aattaataaa ttgattatag atatgatttc ctaaagcttt
      541 tttaataact tcattttcac gcattgcttt taatgcagta taaagtgttg aaggtaagtc
      601 ttgaatgcct actgcttcac gttcttcacg gttcatttcg taaatatttt ggttaactgg
      661 ttctggaact tttaatttat ttttaatacc atctagtcca gcttctaaga ttgcagctaa
      721 cgccatgtat gggtttgcag ctggatctac tgaacgtact tcgatacgag tagataatcc
      781 tcttgaagat ggtacacgga ttaatggtga acggttttta ccactccatg caatataaca
      841 tggtgcttca taaccaggta ctaaacgttt atatgagttt actaacgggt tacatacagc
      901 agtaaatccg cgtgcatttt taagcacacc tgctgtaaat tgatatgcag tttccgttaa
      961 gcccatttca gtatttggat caaagaatgc attttcttta cctttgaata atgaaacgtt
     1021 aaagtgcata ccgctaccat tcacaccgaa taatggttta ggcataaatg ttgcgtgtaa
     1081 attatgttta cgtgcgattg ttttaacaac caatttaaat gtttggatat tatcacatgc
     1141 tgtaacagca tctgcatatt taaagtcaat ttcatgttga ccaggggcaa cttcatggtg
     1201 actagcttca atatcgaagc ccatatcctc taattctaaa acaatatcac gacgacagtt
     1261 ttcacctaaa tctgtaggtg ctaaatcgaa atatccacca tcatcattaa gttctaaagt
     1321 tggttcccct ttttcatcca acttaaacaa gaagaattct ggttcaggcc ctaggttaaa
     1381 gtctgtgaag cctaaatctt ccatttcttt taatacacgt tttaagtttg cacgaggatc
     1441 cccttcaaat ggtgttccat ctgttttata tacatcacaa attaaacgtg caactttacc
     1501 ttgtccagca gtccatggga agattaccca agtatctaaa tctggatgta agtacatatc
     1561 tgattcttcg atacgtacga aaccttcgat agaagaaccg tcaaacatca tttcgttatc
     1621 aagtactttt tctaattggc ttacaggcac ttcaacattt ttaattgttc ctaaaatatc
     1681 agtgaattgt aatcttaaat atcttacatt ttcctcttct gcaaatttac gaatgtcgtc
     1741 tttagtgaaa gtacgttttg gcattttaaa atcctccaaa ttttatttaa taaatcggga
     1801 taaatcacca cgatttattg gcaaagtttc tccaataggc ttttgcgtgg catctacaat
     1861 catctttttt cttatctctt gttcatctgt tgttaaatgc tcttgtgagt cataaatgat
     1921 ttgtttaatc cctttgatat taaatccttt ttctaataat gatttaattt ctagtaatct
     1981 ttccaaatca ttgagtgaga acagacgttt ttgaccttct gttctttcag gtttgatgag
     2041 ttcatgtgtt tcatagtaac gtatttgcct tggcgttaaa tccgttaact tacttactac
     2101 actcatagag aagacagcca tatttcgtct gattgcatca ttcgatatca ttgttcctct
     2161 cctctacttt tgaacttgtt aataacttat cataattttt ttaggatttc aaaaatatgt
     2221 caggttttct aacataaagc ttaaaacatt gatataactg cgatttcgac ttctaaaacg
     2281 acataaaaca atctacagct aaaatatttt ttcaattttt aaaaataatc tgataaatct
     2341 ccgcaaaaaa tttcacccaa aacacattat catttagggt gaaatttaat ctattatata
     2401 agtccttgtt ctttcaactg attaacagct cttgtcacag caattttaac gtgttcatat
     2461 gttaatcctc cttgaacata tgcttcataa ggaggacgaa taggtccatc tgcagataat
     2521 tcaatcgatg aaccttgaat aaacgtacca gctgccataa taacatcatc ttcgtaacca
     2581 ggcatataac taggttctgg actaaaatgt gcattaattg gggatgcgtg ttgaatactt
     2641 tgacaaaatg aaatcatttg ttctttcgtt tcaaatttaa ctgtttgaat taaatcagtt
     2701 cgtttttcgt agtacttcgg cgttgtgttc atattcattt tttctaaaaa taaactagta
     2761 aacaatgcac ctttaagact ctgactgaca acgtgtggtg ctaaaaagaa accttgatac
     2821 atttcaagca atgcatttaa tgatgcaccc gcttctttac caataccagg tgctgtcaat
     2881 ctataaccac atcgttcaat taaatctttt ctaccagcaa tgtatccacc aatcttagct
     2941 aaaccaccgc cagggttttt aattaatgat cctgctatta aatcggcacc acattctata
     3001 ggttcacgtc tttcaacaaa ttccccataa cagttatcca caaatattaa aatattagga
     3061 tgcacgtttt tcaacctagt aattaccttt tcaatttcat ctagcggaat tgaaggtctt
     3121 tgatcatagc ctttcgaacg ttgaatcgct attactttgg tgcgctcaga aaccccatct
     3181 aacacacttt caatatcgat cttaccttct ttaagtgcaa tatctttata cgatacgcca
     3241 tgctccatta aactttcaat accatttccg tttacgccaa tgacttcaag taaagtgtca
     3301 tatggactac ccgttatata aattaattca tcaccatgtt ttaataaact ttgtaatgca
     3361 atagtaatcg catgcgtacc tgaaataatt tgcggacgaa caattgcatc ttctgcttta
     3421 aatgcctgcg catatatttc ttctaaatga tcacgtccaa agtcatcata cccgtatcct
     3481 gttgatcctt gtagatcact ttcggttgct ttgacatgat gaaatgcatt taatactttt
     3541 tcttgattaa tatatgctgt ttcttcaatt tctttaaaat atggtgctaa cgttgattcg
     3601 acgtcagcta ctatcttact tatatctttc atttgaattt cgtcctttta ttatttttta
     3661 aatcctttta tacgataaac attttcaatt tcatcatatt taagttcagt cactaatgta
     3721 tgttgtttta gaaaatataa tcgatctgca ttattcgtcg caattgtctc atcataataa
     3781 gttaattgcc ttttgatttc atcaataaat aacgtcttaa caagtaattt atcaccatca
     3841 tttttagaag aaacaaaaac attaggcaaa tcacttgctg gacgatttga tgcatgatca
     3901 cataagtcct ttttattaaa aataactatt tgagaaatat gactcatatc taattgtttg
     3961 attaaatcat taactgtgtc atactgcgta cggtattcag gatggctact atctacgaca
     4021 tgtactaata aatctgcacc tttagcctct tctaaagttg atttaaaagc tgcaattaac
     4081 gtcgtaggta gtttctgtat aaaaccaaca gtatctgaaa taattaaatt aaatccatca
     4141 tttatttgaa tttgtcgtgt tttaggatct aacgttgcaa ataattgatc tttttcatac
     4201 gtctcttcat ttgctaaaac attaaaccat gatgatttac cagcatttgt ataaccaact
     4261 aaagctactt gaaacacctg attttgattt cttttatttc gatatctttc gcgatgttct
     4321 tctaccgtcc gcaattgatg tttaatttca ttcatacgag ttcgaatatg tctgcgatcc
     4381 atctctaact tcgtttcacc agggcctcta gttccaatac cgccacctaa acgagaaagg
     4441 cttttaccat ggccttgcaa tctaggtaat aaataatcaa gttgtgctag ctctacttgc
     4501 aatttacctt ctttacttct tgctcttaat gcaaatattt caagaatcaa ctgagttcta
     4561 tcaataattt ttacacctaa agcttcattt agtgatttgg attgtgcagt cgttaattca
     4621 tcatttgtga tgactacatc aatatctttg aactcaataa atgcttgaat ttcttcaatt
     4681 ttacctttac caacataata tttgcgatct acacgatctc tgttttgagt aatttgaccc
     4741 aacacttcaa gttggcaagt ctctgataaa gatgataatt cctccattgt agactcaaaa
     4801 ttaaattgct tatcatcttg agcatgtaca ccgactaaga cagctttttc tagtttattt
     4861 ttagtatcat gaatttgttg ctgagccatg ttatacatct ccttaataaa atcctactca
     4921 aagtattcta tcatagagtt ttagtaaaga ctattacacg attttgtggt acattttaag
     4981 taaatcgaaa ggatgataca atggagacaa tttatgattt tgtagtagaa acaaataaag
     5041 gtgttactta caaattagat gcatataagg gtgacgttat gctaattgtt aatacagcta
     5101 gcgaatgtgg ctttacttca caatttgaag gtttgcaatc attatacgag aaatacaaag
     5161 atcaagggtt tgtaattcta ggtttccctt gtaatcaatt tggtggccaa gaacctggtt
     5221 caggcgaaga agcagctcaa aactgtaaat taaactatgg tgttaccttc cctatgcatc
     5281 aaaaaataga cgtaaaaggg gaacatcaat tacctttatt tagatatttg actgcggcac
     5341 aacacggatt ctttaatgag aaaatcaaat ggaatttcac taagttttta gtggatcgcg
     5401 aaggtaacgt tgttaaacga tttgcacctc aaaaaaaacc tgttcaaatc gaaagagaaa
     5461 tagaaaaatt attataaaat atactagctc atagcaaata aaacgcttca tataaagtcg
     5521 tatcatatat tgactcgacc tttatatgaa gcgtttttat tacaaacatt cctctattgt
     5581 atcttgtcat ttgaacatca tttaattgat gtcacatatg actttatttc tatatctatt
     5641 tatcatactt caaacgttat gtacacgagt cattaaatcg tgcccatatg attacaactt
     5701 attcttcact ttcagtagat gcttgacctt cagtttctac tgtataagtg ctgatcgcat
     5761 gtttgtaaat caagtgttgt ttgccttgag aatttaagct tacgacatac ttgtcgtatt
     5821 cttcaataac acctttcatt tggaaaccgt ttagaaagaa tacagttact tcagtttggt
     5881 ttgctttaaa attctctagt gctttgtctt ggatgttttc gtttgcaatc atctgtcgga
     5941 ctccttttac ttaatctggg ttgtaatctc atctaacatc atttgaagtg acatattttc
     6001 tttatctaac caatgaacac tcattttatt cttgaaccat gtcatttgtc gttttgcata
     6061 ttggcgtgaa tgttgcttta aatcattgac agcatcttca taaatcattt gtccgttaat
     6121 cacaggtatt aattctttat atccaatagc ttgcatactt tggcaagatt catagccttg
     6181 ttcaacaagt tgttgcactt ctctaaataa tccgtgatcc aacataatat caacacgttt
     6241 atttattctt gaatataatg ttttacgcga catttcaatc cctaataata atgtatcata
     6301 attttcagta aattgttgca ctttcttgcg attactcaaa agtttttttg tttttaaata
     6361 atattcaata gcgcgcaaca ctctttggcg gttgttaggg tgaatatttt ctgcagaaac
     6421 cgcatcaaat tgagctaaat aatcgtgtag ttgctgatta tctaaatgtt ctaatgcaga
     6481 taacttttgt ttaactatgg ataattgtgc aggtgtaact gtttcatctt ctaattcata
     6541 attatatatt aatgattgaa tatataagcc tgttccacct gctatgattg gaactttacc
     6601 tctattcgtt atatcagtaa ttaaatcttc tgctaatcgc ttgaattcat atgctgaaaa
     6661 tgtatcatca ggattcaaga tatcaattaa atgatgtgga ataccatcca tttcttcagg
     6721 tgttactttt gcagttccaa tattcatatg tttgtagact tgcatagagt caccgcttat
     6781 gatttcacca ttgatacgct tcgcgagttc gatgctaagc tctgttttac ctgaagcagt
     6841 tggccccaca attactacaa taaaaggctt atttttattc atatttaatt ccactttctt
     6901 tattttttat aaaattgatt caaccattta aacaagtctt cccaaatttt gtcatgatct
     6961 ttttcaaata acacttcatg acgtttatga tgataaagat tcactttaac atttttaatg
     7021 ccagcttttc tatatatatt cgccaatttt aaaatccctt taccataatc acctaaagga
     7081 tcgtcatatc ctgaaactaa taatataggc atggcatgat ttaacatttt catatttttt
     7141 aattgtgatg tatgtagcat ataataggct gtttgatata ataattgatt tgacacatta
     7201 aatccactat atggatcttt aataaagtta tctacttcaa ttggattact agaaatccaa
     7261 tcactttgtg tacgtaatgg acgtattttt ttattaaaac tatttgatac taactggtta
     7321 acccattcaa ctcgtttctc agcaccataa atttttgtaa tcagttgtaa cactttaacg
     7381 gttggtaaac ctttccataa tgaatacata ccagtaccac ttaaaattag accatcaaca
     7441 tattgcggat atgtttctac aaacaatcta gctataacgg atcccattga atgtccgatt
     7501 ataatgtatg gtttatcaac attgcctctc actgtttgcg ctacttcaaa ggcatcaccg
     7561 ataactcgtt tcatatcatc gtaatgccct cttgttgatt catcaatatt aataccatgt
     7621 cctcgatgat tatgacgtat cacatcgaag ccatgctttg aaagtgcatg tgctaattta
     7681 tcgtaacgtt ccatatgttc agccatacca tgaaatatat gaataattcc ttttgaatct
     7741 acatctgtaa aattcacttt aacttcaata ttcgtattat cttgtacagt taatttaaat
     7801 tgtttttcag tcatcatctc acacctttaa taacaacgct tttatattcg cttttattat
     7861 tatcatacat ttagtgatgc atagtttcaa ttgactaatg tatctcgtat aaaaaaactg
     7921 atgcccttaa aacaggacac cagtttatga ttgtacaaat taattattct ttaactgctg
     7981 gttgattatt tccatgttgc gcttctttaa ttgcttgctc aacttcttca gtatatgctt
     8041 caatttgagc tggagagtaa tcaagcatat ctgccataat atcgatgaca gcatctttat
     8101 aatctaatac atctttaata ttgaaataca ttttaccaga acgacgaact aagaaatcgt
     8161 taggtttgta taccatttct tgttgaatac tataaacaag ttctacataa atttctaatg
     8221 gtaacttgct atcatggtat tgagatgttt gcgcaatgtt gaacaattca tcaacatttg
     8281 aaccatattt agatgctaaa cgtcttgcaa catcttcatc aatgccgaat cctttagcta
     8341 catctacttt ttgctctaca aacgcatcaa agttcttgct accacctaca tcgccacctg
     8401 aaattgccag accttttgta ttacatggac taaatgttaa accgtagtct tttttcaagc
     8461 gtttagatac taaatcaaca atgtcttgag ccatgtgacg atagcctgtt aatttaccac
     8521 ctgcaatagt taataaacct gatttacctt cccaaatttc atccttacga gagatttcag
     8581 aagggtcttt gccttcttcg taaattaatg gtctaattcc tgcccatgtt gattcaatat
     8641 cttcatctgt aacattaaca ctagggaaca tgtaattaat cgcatcgatt aaatagtctc
     8701 tgtcttcttg tgtagttaat ggtgaagatt tgatattgtc atagaatgta tctgtagtac
     8761 ctacatacgc ttttccttca cgtggaattg caaaaatcat tcttccatct ttttcagtat
     8821 caaagtatac tgcttgacct aatgggaatt ttgattgatc aataacaaca tgtacacctt
     8881 tagttaaacg taatttttta ttattgcgtg cataatcacc acttctaaca tcatcaaccc
     8941 atggacctgc tgcattaacc acttttttag ccttaattgt ataattttca ttagttaatt
     9001 tatctataac tttaacacca tttacttgtt gatttttatc ataagtgaag tgttcagatt
     9061 tagtatagtt gataatttct gcgccttttt cagcagcacg cttcataact tcaatagtta
     9121 aacgcgcatc gtcagtacga tattcaacat agtaaccgcc gccttttaga ccttcttttt
     9181 taactaatgg ttctttagct aaagtttctt ttttagataa cattttttta cgttcagact
     9241 tcttaacacc tgctaaacga tcatacattc ctaaaccaat tgatgttgag aatttaccaa
     9301 atgttccacc tttatgcatt ggtaaaagca tccactctgg agtcgtaaca tgaggcccat
     9361 tttcataaac aatcgcacgt tctttaccag tttcggcaac tactccaatt tggaattgtt
     9421 ttaagtaacg caaaccacca tggactaatt ttgtagatct tgagcttgtt ccttgtgcaa
     9481 agtcttgcat ttcaactaat gcaactttca ttcctctttc actcgcgtct agtgcaatac
     9541 ctgcacctgt aataccgcca ccaataatta ctaaatcata ttcatcattt cttaaattct
     9601 ttttaatatg ttctctctta aaagtagaca atgccattcc aaaacgcctc ctaaaaaatg
     9661 taaaaacgag agacttgtcc cacataaaca atccttcaat tgtacatgtt cgaacaaatc
     9721 tctcgcttct cgattcaaat attaacttaa attgattgta tcacaatgtt tatacaaaag
     9781 tctaatctac aagtttattc tgttttaaaa acttgtgttg cttcaacagc ttttttccaa
     9841 cctctatata atttttctct ttcgccttca tccattttcg gatcgaattt ttcttctaat
     9901 ttccagtttt tagcgatatc atctttactc tcccagaatc caactgctaa acctgctaaa
     9961 aatgcagcac ctaaagctgt agtttcttga atttcaggtc tttcaacaga agtattaaca
    10021 atgtctgctt ggaactgcat aataaagtta tttttaactg caccaccatc gacacgtaaa
    10081 ctttggacat caataccaga gtcttttgac attgcttcca taacgtcacg agtttggtaa
    10141 catagtgatt ctaaagttgc acggataaag tgctcttttt cagttccacg tgttaaaccg
    10201 aaaatcgcac cacgtgcttc agaatcccaa tatggtgttc ctaaacctac aaaagctgga
    10261 acaacataaa caccctcagt agagtcaact cgtgtcgcat aactttctga ttgtggtgct
    10321 gaattaatca ttcttaatcc atcacgtaac cattggattg ctgaacccga aacaaagatg
    10381 gaaccttcaa gcgcataatt tacttttcca tcaataccat aagcaattgt tgttaataaa
    10441 ccactttcag atttaaccgc tttgtcacct gtattcatta acatgaagcc accagttcca
    10501 tatgtgtttt tcacgtcacc acgttcgaag caagcttgtc caaataatgc tgcttgttga
    10561 tcaccagcta ctccagcgat tggtacttct tgaccataga agtggtaatc aattgtctta
    10621 ccatatactt cactcgaagc tttaacttct ggcaacatat ttttaggtac tgtaagtagt
    10681 tctaataact catcgtccca ttctaaatca tggatattaa acattaatgt acgactcgca
    10741 tttgaataat cagtaatatg cgcagctttt cctgataatt tccatactaa ccaagtatca
    10801 atcgttccaa ataatagatc gccattttct gctttttctc gtgcaccttc aacattgtct
    10861 agaatccatt taacttttgt acctgcaaaa tacggatcta aaagtaatcc tgtcttatct
    10921 ctaaatgttt gttcatatcc ttgttgtttt aattctgaac aaattgattg tgtttgacgt
    10981 gattgccaaa caattgcgtg ataaattggg cggccagtat gtttgtccca aacaaccgtt
    11041 gtttcacgtt ggtttgtaat accgatacct gcaatttgat cagctctaac atcattttca
    11101 ttaattactt ccgtcattac agctaacaca gatgtccaaa tttcatttgc atcatgttca
    11161 acccaacctg attgtggaaa atattgctta aactcacgtt gtgctacccc tgcaatttcc
    11221 cctttttgat tgaataaaat cgctcttgag cttgttgttc cttggtctat agataaaata
    11281 tatttttcca ttagtagtat ctcctttatt aaaaaataat cgctaaccca ttgtacaaaa
    11341 tctgttaaat tttggcgaaa aaata
//
LOCUS       DAKTIP010000074        11334 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAKTIP010000074 DAKTIP010000000
VERSION     DAKTIP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.074"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            33..2108
                     /gene="topA"
                     /locus_tag="O0305_001441"
     CDS             33..2108
                     /gene="topA"
                     /locus_tag="O0305_001441"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCY0274589.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            2264..3571
                     /gene="trmFO"
                     /locus_tag="O0305_001442"
     CDS             2264..3571
                     /gene="trmFO"
                     /locus_tag="O0305_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCY0274590.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            3988..4884
                     /gene="xerC"
                     /locus_tag="O0305_001443"
     CDS             3988..4884
                     /gene="xerC"
                     /locus_tag="O0305_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCY0274591.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            4881..5426
                     /gene="hslV"
                     /locus_tag="O0305_001444"
     CDS             4881..5426
                     /gene="hslV"
                     /locus_tag="O0305_001444"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCY0274592.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            5492..6895
                     /gene="hslU"
                     /locus_tag="O0305_001445"
     CDS             5492..6895
                     /gene="hslU"
                     /locus_tag="O0305_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCY0274593.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            6920..7693
                     /gene="codY"
                     /locus_tag="O0305_001446"
     CDS             6920..7693
                     /gene="codY"
                     /locus_tag="O0305_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCY0274594.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            7744..7836
                     /locus_tag="O0305_001447"
     CDS             7744..7836
                     /locus_tag="O0305_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274595.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            8035..8802
                     /gene="rpsB"
                     /locus_tag="O0305_001448"
     CDS             8035..8802
                     /gene="rpsB"
                     /locus_tag="O0305_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCY0274596.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            8836..8949
                     /locus_tag="O0305_001449"
     CDS             8836..8949
                     /locus_tag="O0305_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274597.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            8984..9865
                     /locus_tag="O0305_001450"
     CDS             8984..9865
                     /locus_tag="O0305_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCY0274598.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            10002..10724
                     /locus_tag="O0305_001451"
     CDS             10002..10724
                     /locus_tag="O0305_001451"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCY0274599.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            10743..11297
                     /gene="frr"
                     /locus_tag="O0305_001452"
     CDS             10743..11297
                     /gene="frr"
                     /locus_tag="O0305_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCY0274600.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
BASE COUNT         4298 a         1621 c         2238 g         3177 t
ORIGIN      
        1 ttatctttag taattagatt agcgaggggg aaatgacatt ggcagataat ttagtcattg
       61 ttgaatcgcc tgcaaaagca aaaaccattg aaaagtattt aggtaagaaa tataaagtta
      121 tagcttcaat gggacacgtc agagacttac caagaagtca aatgggtgtc gacactgaag
      181 ataattacga accaaaatat ataacaatac gcggaaaagg tcctgttgta aaagaattga
      241 aaaaacatgc aaaaaaagcg aaaaacgtct ttctcgcaag tgaccccgac cgtgaaggtg
      301 aagcaattgc ttggcattta tcaaaaattt tagagcttga agattctaaa gaaaatcgcg
      361 ttgttttcaa cgaaataact aaagacgctg ttaaagaaag ttttaaaaat cctagagaaa
      421 ttgaaatgaa cttagtcgat gcacaacaag cgcgtcgaat attagataga ttagttggct
      481 ataacatctc gccagttctt tggaaaaaag taaaaaaagg gttgtcagcg ggtcgagttc
      541 aatctgttgc acttcgttta gtcattgacc gtgaaaatga aattcgaaac tttaaaccag
      601 aagaatattg gactattgaa ggagaattta gatacaaaaa atcaaaattc aatgctaaat
      661 tccttcatta taaaaataaa ccttttaaac taaaaacgaa aaaagatgtt gagaaaatta
      721 cagctgcatt agatggagat caattcgaaa ttacaaacgt gactaaaaaa gaaaaaacgc
      781 gtaatccagc aaacccattt acaacttcta cattacaaca agaggcggca cgtaaattaa
      841 actttaaagc aagaaaaaca atgatggtcg cacaacaatt atatgaaggt atagatttga
      901 aaaaacaagg tacgattggt ttaataacat atatgagaac cgattctaca cgtatttcag
      961 atactgccaa agttgaagca aaacagtata taactgataa atacggtgaa tcttacactt
     1021 ctaaacgtaa agcatcaggg aaacaaggtg accaagatgc ccatgaggct attagacctt
     1081 caagtactat gcgtacgcca gatgatatga agtcattttt gacgaaagac caataccgat
     1141 tatacaaatt aatttgggaa cgatttgttg ctagtcaaat ggctccagca atacttgata
     1201 cagtctcatt agacataaca caaggtgaca ttaaatttag agcgaatggt caaacaatca
     1261 agtttaaagg atttatgaca ctttatgtag aaactaaaga tgatagtgat agcgaaaagg
     1321 aaaataaact gcctaaatta gagcaaggtg ataaagtcac agcaactcaa attgaaccag
     1381 ctcaacacta tacacaacca cctccaagat atactgaggc gagattagta aaaacactag
     1441 aagaattgaa aattgggcga ccatcaactt atgcaccgac aatagatacg attcaaaagc
     1501 gtaactatgt caaattagaa agtaagcgtt ttgttcctac tgagttggga gaaatagttc
     1561 atgaacaagt gaaagaatac ttcccagaga ttattgatgt ggaattcaca gtgaatatgg
     1621 aaacgttact tgataagatt gcagaaggcg acattacatg gaggaaagta atcgacggtt
     1681 tctttagtag ctttaaacaa gatgttgaac gtgctgaaga agagatggaa aagattgaaa
     1741 tcaaagatga gccagccggt gaagactgtg aaatttgtgg ttctcctatg gttataaaaa
     1801 tgggacgcta tggtaagttc atggcttgct caaacttccc ggattgtcgt aatacaaaag
     1861 cgatagttaa gtctattggt gttaaatgtc caaaatgtaa tgatggtgac gtcgtagaaa
     1921 gaaaatctaa aaagaatcgt gtcttttatg gatgttcgaa atatcctgaa tgcgacttta
     1981 tctcttggga taagccgatt ggaagagatt gtccaaaatg taaccaatat cttgttgaaa
     2041 ataaaaaagg caagacaaca caagtaatat gttcaaattg cgattataaa gaggcagcgc
     2101 agaaataata tttttatttc ctagatacat tttaagattg ttaaatagaa tcattagtga
     2161 atcttatttt aaagatagta aaggattaat ctaaataagt gcggataata taaacataac
     2221 aacataatta aaagacataa atgacaataa aaggagtata gaaatgactc aaactgtaaa
     2281 tgtaataggt gctggtcttg ccggttcaga agcggcatat caattagctg aaagaggaat
     2341 taaagttaat ctaatagaga tgagacctgt taaacaaaca ccagcgcacc atactgataa
     2401 atttgcggaa cttgtatgtt ccaattcatt acgcggaaat gctttaacta atggtgtggg
     2461 tgttttaaaa gaagaaatga gaagattgaa ttctataatt attgaagcgg ctgataaggc
     2521 acgagttcca gctggtggtg cattagcagt tgatagacac gatttttcag gttatattac
     2581 tgaaacactt aaaaatcatg aaaatatcac agttattaat gaagaaatta atgccattcc
     2641 agatggatac acaattatcg caacaggacc acttactaca gaaacccttg cgcaagaaat
     2701 agtggacatt actggtaaag atcaacttta tttctatgat gcggctgctc caattattga
     2761 aaaagaatct attgatatgg ataaagttta cttaaagtcc cgttatgata aaggtgaagc
     2821 tgcatattta aactgtccta tgactgagga tgaatttaat cgcttttatg atgcagtatt
     2881 agaagctgaa gttgcgcctg taaattcatt tgaaaaagaa aaatatttcg agggttgtat
     2941 gccttttgaa gtaatggcag aacgcggacg caagacatta ctatttggac caatgaaacc
     3001 agtaggatta gaagatccaa agactgggaa acgtccttat gcggtggttc aattaagaca
     3061 agatgacgct gctggtacac tctacaatat tgttggcttc caaacgcatt taaaatgggg
     3121 agctcaaaaa gaagtcatta aattaattcc aggcttagaa aatgttgata ttgttagata
     3181 tggtgtgatg catagaaata ccttcattaa ttcaccggac gtattaaacg agaaatatga
     3241 attgatttca caaccaaaca tacagtttgc gggacaaatg actggtgttg aaggttatgt
     3301 agaaagcgca gctagcggct tagttgcagg tatcaatctt gcgcataaaa tattaggcaa
     3361 gggtgaggta gtatttccga gagaaacaat gattggaagt atggcttact atatttctca
     3421 tgctaaaaac aataagaatt tccaacctat gaatgctaac ttcgggttat taccatcttt
     3481 agaaactaga attaaagata aaaaagaacg ctatgaagca caagctaata gagctttgga
     3541 ttacttagaa aatttcaaaa aaactttata aaatagttag aaagactaga tatgctattc
     3601 attcttaagt catcaacgag taagtaatga ctttctaaat ggaaaatact tatcctagtc
     3661 tttttaattt tggaattgtt acgtatttct gacaatttag aattcgcatt caaaaaatat
     3721 ctaaataaat aacacgcaat aagttgattg atgtaacatg taagagaatg ttttaaataa
     3781 actttattta aaaggcaatg aaataataaa tggcaaggct attaataaag acttttagta
     3841 attaatttaa aaaagaggta ttctaattaa caggttttcc gattagttac aattatttaa
     3901 ttctcaaaag atttagaatt gattatcaaa ttactgtaag ccctttgctg tatatgctac
     3961 aattcttatt gatggagggt aaatgtattg aatcatattc aagatgcgtt tttaaataca
     4021 ttgaaagttg aacggaattt ttcggaacac acattgaaat catatcaaga tgacttaatt
     4081 cagtttaatc aatttttaga acaagaacat ttagagttga atacttttga atacagagat
     4141 gctagaaatt atttgagcta tttatattca aatcatttga aaagaacatc tgtttctcgt
     4201 aaaatctcaa cgttaagaac tttctatgaa tattggatga cgcttgatga gaacattatt
     4261 aatccatttg ttcaattagt acatccgaaa aaagaaaaat atcttccgca attcttttac
     4321 gaagaagaaa tggaagcgtt attcaaaact gtagaagagg acacttcaaa aaatttacgg
     4381 gatcgagtta ttcttgaatt gttgtatgct acaggcatcc gtgtttcgga attagtaaat
     4441 attaaaaaac aagatataga tttttacgcg aatggtgtta ccgtattagg aaaagggagc
     4501 aaagagcgct ttgtaccgtt tggtgcttat tgtagacaaa gcatcgaaaa ttatttagaa
     4561 catttcaaac caattcagtc atgcaatcat gattttctta ttgtaaatat gaagggtgaa
     4621 gcaatcactg aacgcggtgt acgatatgtt ttaaatgata ttgttaaacg aacagcaggc
     4681 gtaagtgaga ttcatcccca caagctcaga catacatttg caacgcattt attgaatcaa
     4741 ggtgcagacc taagaacagt acaatcgtta ttaggtcatg ttaatttgtc aacaactggt
     4801 aaatatacac acgtatctaa ccaacaatta agaaaagtgt atctaaatgc acatcctcga
     4861 gcgaaaaagg agaatgaaac atgagtaata caacattaca tgcaacaaca atttatgctg
     4921 taagacataa tgggaaagca gctatggctg gagatgggca agtaacgctt ggtcaacaag
     4981 tcatcatgaa acaaacggca agaaaagtgc gacgtttata tgaaggtaaa gtgttagctg
     5041 gtttcgcagg tagtgtagca gatgcgttta cgttatttga aaaattcgaa acaaaattac
     5101 aacagtttag tggtaactta gaaagagctg ctgttgaatt ggcacaagaa tggcgaggcg
     5161 ataaacaatt acgtcaatta gaagctatgc taattgtaat ggataaagat gctattttag
     5221 ttgtcagtgg aactggcgaa gttattgctc cagatgatga ccttatcgct attggatcag
     5281 gaggcaacta cgcattaagc gcaggacgtg cattgaaacg ccatgcatcg catttgtctg
     5341 ctgaagaaat ggcatatgag agcttgaaag tagcggctga tatttgtgtc tttaccaacg
     5401 ataatattgt tgtcgaaaca ctataataat cagagcacga taaataatta cgagcaatta
     5461 attttagtta aaagacggag gaatgaaatt aatggataca gctggaataa gattaactcc
     5521 aaaagaaatc gtatctaaat taaatgaata catcgttgga caaaatgatg ctaaacgtaa
     5581 agtggcaatt gccctacgta atcgatacag aagaagttta ttagatgagg aatcaaagca
     5641 agaaatttca cctaaaaata ttttgatgat tggaccaacc ggcgttggta aaactgaaat
     5701 tgcaagaaga atggccaaag ttgtcggcgc gccatttata aaagtagaag ctactaaatt
     5761 tactgaggta ggttatgtag gacgagatgt tgaaagtatg gttagagatc ttgttgatgt
     5821 ttcagtaaga ttagtcaagg cgcagaaaaa atcattggta caagatgaag caacagctaa
     5881 ggccaatgaa aaacttgtta agttattagt tccaagtatg aaaaagaaag cgtctcaaac
     5941 gaataatcct ttagagtcac ttttcggagg tgcaattcca aatttcggac aaaataacga
     6001 agatgaagaa gaaccaccta ctgaggaaat taaaacaaaa cgttctgaaa ttaagagaca
     6061 gctagaagaa ggcaaacttg aaaaagaaaa ggtaagaatt aaagtcgaac aagatcctgg
     6121 tgctttaggt atgctaggta caaatcaaaa tcagcaaatg caagagatga tgaatcaatt
     6181 aatgcctaaa aagaaagttg agcgagaagt tgctgttgag acggcaagga aaatcttagc
     6241 tgatagttat gcggatgaac taattgatca agaaagcgct aaccaagaag cgcttgaatt
     6301 agcagaacaa atgggtatca tctttataga tgaaatcgac aaagttgcga cgaataatca
     6361 taatagtggt caagatgtct caagacaagg tgttcaaaga gatattttac ctatacttga
     6421 aggtagcgtt attcaaacca aatatggtac tgtgaatact gaacatatgc tgtttatagg
     6481 tgctggagct ttccatgtat ctaagccgag tgacttgata ccagaattgc aaggtcgttt
     6541 tccgattaga gttgaacttg atagtttatc ggtagaagat tttgtaagaa ttttgacaga
     6601 accaaaattg tcattaatta aacaatatga agcattgctt caaacagaag aagttactgt
     6661 aaactttacc gatgaagcaa ttactcgctt agctgagatt gcttatcaag taaatcaaga
     6721 tacagacaac attggtgcac gtcgacttca tacaatttta gaaaagatgc tagaagattt
     6781 atcattcgaa gcaccaagta tgccgaatgc agttgtagat attaccccac aatatgttga
     6841 tgataaatta aaatcaattt caacaaataa agatttaagt gcatttattc tataaaaata
     6901 tacaaaagga gaaaaattca tgagcttatt atctaaaacg agagagttaa acacgttact
     6961 tcaaaaacac aaaggtattg cggttgattt taaagatgta gcacaaacga ttagtagcgt
     7021 aactgtaaca aatgtattta ttgtatcgcg tcgaggtaaa attttaggat cgagtctaaa
     7081 tgaattatta aaaagtcaaa gaattattca aatgttggaa gaaagacata ttccaagtga
     7141 atatacagaa cgattaatgg aagttaaaca aacagaatca aatattgata tcgacaatgt
     7201 attaacagta ttcccacctg aaaacagaga attattcata gatagtcgta caactatctt
     7261 cccaatttta ggtggagggg aaagattagg tacattagta cttggtcgag tacatgatga
     7321 ttttaatgaa aatgatttgg tactaggtga atatgctgct acagttattg gtatggaaat
     7381 cttacgtgag aagcatagtg aagtagaaaa agaagcgcgc gataaagctg ctattacaat
     7441 ggcaattaat tcattatctt attctgaaaa agaagcgatt gaacatatct ttgaagaact
     7501 tggcggtacg gaaggcctat taatcgcatc aaaagttgca gatagagttg gtattactag
     7561 atctgtaatt gtaaatgcac tacgtaaatt agaaagtgct ggtgtaattg aatcacgttc
     7621 tttaggaatg aaaggtactt tcattaaagt taaaaaagaa aaattcttag atgaattaga
     7681 aaaaagtaaa taagtcgatg agtctgggac ataattcaat gtcctagtcg tgacgatgtt
     7741 atcttggcgg tagtcaatga aatgactttg atgattatgt tgaagtcatt tctttatttt
     7801 tgtctaattt tcaatcaaat tgatatttca cattaacttg atagaattac ctttaaattt
     7861 atggaaaaat gcttgaaaaa catttgcttt agacacggca ttatgttata tttattttcg
     7921 gctataaagc caatacacac atatcaaacg tgattgtgta aagggtgcaa tatttatata
     7981 ttgtctttac aatagtttgc tatggaggta attaaccaat aggaggaatt tataatggca
     8041 gtaatttcaa tgaaacaatt actagaagcg ggtgttcact tcggtcacca aacacgtcgt
     8101 tggaacccaa aaatgaaaaa atatatcttc actgagagaa atggtattta tatcatcgac
     8161 ttacaaaaaa cagtgaaaaa agtagacgag gcatacaact tcttgaaaca agtttcagaa
     8221 gatggtggac aagtcttatt cgtaggaact aaaaaacaag cacaagaatc agttaaatct
     8281 gaagcagaac gtgctggtca attctacatt aaccaaagat ggttaggtgg attattaact
     8341 aactataaaa cgatctcaaa acgaatcaaa cgtatttctg aaattgaaaa aatggaagaa
     8401 gatggtttat tcgaagtatt acctaaaaaa gaagtagtag aacttaaaaa agaatacgac
     8461 cgtttaatca aattcttagg cggaattcgt gatatgaaat caatgcctca agcattattc
     8521 gtagttgacc cacgtaaaga gcgtaatgca attgctgaag ctcgtaaatt aaatattcct
     8581 atcgtaggta tcgttgacac taactgtgat cctgacgaaa ttgactacgt tatcccagca
     8641 aacgacgatg ctatccgtgc ggttaaatta ttaactgcta aaatggcaga tgcaatctta
     8701 gaaggtcaac aaggcgtttc taatgaagaa gtagctgcag aacaaaacat cgatttagat
     8761 gaaaaagaaa aatcagaaga aacagaagca actgaagaat aatcaactgt tgaatctgac
     8821 ttagatatag tttaaatggg tgataagata ttaatgctta tcaccttttt taaaaagaaa
     8881 atcgaggcaa attacaaata ttcaattaga gtattggcaa tcttgcctat aataatgcta
     8941 aaatcataat atataaaatg ataacttatt ggaggaataa tgaatggcaa ctatttcagc
     9001 aaaacttgtt aaagaattac gtgaaaaaac tggcgcgggt atgatggatt gtaaaaaagc
     9061 gctaactgaa actgatggtg acatcgataa agcgattgac tacctacgtg aaaaaggtat
     9121 tgctaaagca gctaaaaaag cagaccgtat tgcggctgaa ggtttagtac atgtagaaac
     9181 taaaggtaac gacgcagtta tcgttgaaat caactctgaa acagactttg ttgctcgtaa
     9241 cgaaggtttc caagagttag ttaaagaaat cgctaatcaa gtattagata caaaagctga
     9301 aactgttgaa gctttaatgg aaacaacttt accaaatggt aaatcagttg atgaaagaat
     9361 taaagaagca atttcaacaa tcggtgaaaa attaagtgtt cgtcgttttg ctatcagaac
     9421 taaaactgat aacgatgctt tcggcgctta cttacacatg ggtggacgca ttggtgtatt
     9481 aacagttgtt gaaggttcaa ctgacgaaga agcagcaaga gacgttgcta tgcatatcgc
     9541 tgcaatcaac cctaaatatg tttcttctga acaagttagc gaagaagaaa tcaaccacga
     9601 aagagaagtt ttaaaacaac aagcattaaa tgaaggtaaa ccagaaaaca tcgttgaaaa
     9661 aatggtggaa ggacgtttac gtaaatactt acaagaaatt tgtgctgtag atcaagactt
     9721 cgttaaaaac cctgatgtaa cagttgaagc tttcttaaaa acaaaaggtg gaaaacttgt
     9781 tgacttcgta cgctatgaag taggcgaagg tatggaaaaa cgcgaagaaa actttgcgga
     9841 tgaagttaaa ggacaaatga aataatctgt cataaagtaa aacaaggaag aagacacctt
     9901 taatgttgct ttattaaaat gtaaatcatt ctaataaaac gacaactgtg tcttctttac
     9961 ttgtatatgt tacatatatt cacgatagag aggataagaa aatggctcaa atttctaaat
    10021 ataaacgtgt agttttgaaa ctaagtggtg aagcgttagc tggagaaaaa ggatttggca
    10081 taaatccagt aattattaaa agtgttgctg agcaagtggc tgaagttgct aaaatggact
    10141 gtgaaatcgc agtaatcgtt ggtggcggaa acatttggag aggtaaaaca ggtagtgact
    10201 taggtatgga ccgtggaact gctgattaca tgggtatgct tgcaactgta atgaatgcct
    10261 tagcattaca agatagttta gaacaattgg attgtgatac acgagtatta acatctattg
    10321 aaatgaagca agtggctgaa ccttatattc gtcgtcgtgc aattagacac ttagaaaaga
    10381 aacgcgtagt tatttttgct gcaggtattg gaaacccata cttctctaca gatactacag
    10441 cggcattacg tgctgcagaa gttgaagcag atgttatttt aatgggcaaa aataatgtag
    10501 atggtgtata ttctgcagat cctaaagtaa acaaagatgc ggtaaaatat gaacatttaa
    10561 cgcatattca aatgcttcaa gaaggtttac aagtaatgga ttcaacagca tcctcattct
    10621 gtatggataa taacattccg ttaactgttt tctctattat ggaagaagga aatattaaac
    10681 gtgctgttat gggtgaaaag ataggtacgt taattacaaa ataaatttag aggtgtaaaa
    10741 taatgagtga cattattaat gaaactaaat caagaatgca aaaatcaatc gaaagcttat
    10801 cacgtgaatt agctaacatc agtgcaggaa gagctaattc aaatttatta aacggcgtaa
    10861 cagttgatta ctatggtgca ccaacacctg tacaacaatt agcaagcatc aatgttccag
    10921 aagcacgttt acttgttatt tctccatacg acaaaacttc tgtagctgac atcgaaaaag
    10981 cgataatagc agctaactta ggtgttaacc caacaagtga tggtgaagtg atacgtattg
    11041 ctgtacctgc cttaacagaa gaacgtagaa aagagcgcgt taaagatgtt aagaaaattg
    11101 gtgaagaagc taaagtatct gttcgaaata ttcgtcgtga tatgaatgat cagttgaaaa
    11161 aagatgaaaa aaatggcgac attactgaag atgagttgag aagtggtact gaagatgttc
    11221 agaaagcaac agacaattca ataaaagaaa ttgatcaaat gattgctgat aaagaaaaag
    11281 atattatgtc agtataaaac taatatacaa tgacatatta aaatgccagt atta
//
LOCUS       DAKTIP010000075        11280 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAKTIP010000075 DAKTIP010000000
VERSION     DAKTIP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.075"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(141..326)
                     /locus_tag="O0305_001453"
     CDS             complement(141..326)
                     /locus_tag="O0305_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCY0274601.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            621..1562
                     /locus_tag="O0305_001454"
     CDS             621..1562
                     /locus_tag="O0305_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCY0274602.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASLKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSDGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKIKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            1574..2818
                     /gene="fabF"
                     /locus_tag="O0305_001455"
     CDS             1574..2818
                     /gene="fabF"
                     /locus_tag="O0305_001455"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCY0274603.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVNS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(2873..3244)
                     /locus_tag="O0305_001456"
     CDS             complement(2873..3244)
                     /locus_tag="O0305_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCY0274604.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            3487..4413
                     /locus_tag="O0305_001457"
     CDS             3487..4413
                     /locus_tag="O0305_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274605.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            4413..5483
                     /locus_tag="O0305_001458"
     CDS             4413..5483
                     /locus_tag="O0305_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274606.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            5500..6582
                     /locus_tag="O0305_001459"
     CDS             5500..6582
                     /locus_tag="O0305_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274607.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLKVERVSFNEK"
     gene            6572..7513
                     /locus_tag="O0305_001460"
     CDS             6572..7513
                     /locus_tag="O0305_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0274608.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPAEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            7532..9187
                     /locus_tag="O0305_001461"
     CDS             7532..9187
                     /locus_tag="O0305_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0274609.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            9399..11114
                     /locus_tag="O0305_001462"
     CDS             9399..11114
                     /locus_tag="O0305_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0274610.1"
                     /translation="MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            11165..>11280
                     /locus_tag="O0305_001463"
     CDS             11165..>11280
                     /locus_tag="O0305_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274611.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLG"
BASE COUNT         4099 a         1588 c         2159 g         3434 t
ORIGIN      
        1 taaatttaaa agtgatgggt tagaggtcat tgagctttaa aatattcaaa atacaaaaca
       61 ttaatggcca aaaataaaag ccgcctttat ctgggcagct tcaataataa gaaagacata
      121 tttcatttta tactaaatag ttattgtgat gaatctttcg gcggtttaat tactgcagca
      181 aaaattgctg tgaaaatcgt gaacaatact gccatgataa ttggattcac tacatttaag
      241 ctgtctccac ctactaggct attaagtaca aagttaacca tttgcattaa taataatgcc
      301 caaaagaatg ttacgaggtg tttcatgtca ttctacctcc actttaatta tatatatttt
      361 attttaagtg aaagttagaa atttgtatag taacatctca tatattttga ccatattata
      421 cagtttaaat aaatgatttt atctgaatgg ctattctaaa ttaagcgcat taaaaccaat
      481 ttcatactga aatttgacga taataaagca ttaaaatttt attaactagt caatattcct
      541 acctctgact tgagtttaaa aagtaatcta tgttaaatta atacctggta ttaaaaattt
      601 tattaagaag gtgttcaact atgaacgtgg gtattaaagg ttttggtgca tatgcgccag
      661 aaaagattat tgacaatgcc tattttgagc aatttttaga tacatctgat gaatggattt
      721 ctaagatgac tggaattaaa gaaagacatt gggcagatga tgatcaagat acttcagatt
      781 tagcatatga agcaagttta aaagcaatcg ctgacgctgg tattcagccc gaagatatag
      841 atatgataat tgttgccaca gcaactggag atatgccatt tccaactgtc gcaaatatgt
      901 tgcaagaacg tttagggacg ggcaaagttg cctctatgga tcaacttgca gcatgttctg
      961 gatttatgta ttcaatgatt acagctaaac aatatgttca atctggagat tatcataaca
     1021 ttttagttgt cggtgcagat aaattatcta aaataacaga tttaactgac cgttctactg
     1081 cagttctatt tggagatggt gcaggtgcgg ttatcatcgg tgaagtttca gatggcagag
     1141 gtattataag ttatgaaatg ggttctgatg gcacaggtgg taaacattta tatttagata
     1201 aagatactgg taaactgaaa atgaatggtc gagaagtatt taaatttgct gttagaatta
     1261 tgggtgatgc atcaacacgt gtagttgaaa aagcgaattt aacatcagat gatatagatt
     1321 tatttattcc tcatcaagct aatattagaa ttatggaatc agctagagaa cgcttaggta
     1381 tttcaaaaga caaaatgagt gtttctgtaa ataaatatgg aaatacttca gctgcgtcaa
     1441 tacctttaag tatcgatcaa gaattaaaaa atggtaaaat caaagatgat gatacaattg
     1501 ttcttgtcgg attcggtggc ggcctaactt ggggcgcaat gacaataaaa tggggaaaat
     1561 aggaggataa cgaatgagtc aaaataaaag agtagttatt acaggtatgg gagccctttc
     1621 tccaatcggt aatgatgtca aaacaacatg ggagaatgct ctaaaaggcg taaatggtat
     1681 cgataaaatt acacgtatcg atactgaacc ttatagcgtt cacttagcag gagaacttaa
     1741 aaactttaat attgaagatc atatcgacaa aaaagaagcg cgtcgtatgg atagatttac
     1801 tcaatatgca attgtagcag ctagagaggc tgttaaagat gcgcaattag atatcaatga
     1861 aaatactgca gatcgaatcg gtgtatggat tggttctggt atcggtggta tggaaacatt
     1921 tgaaattgca cataaacaat taatggataa aggcccaaga cgtgtgagtc catttttcgt
     1981 accaatgtta attcctgata tggcaactgg gcaagtatca attgacttag gtgcaaaagg
     2041 accaaatggt gcaacagtta cagcatgtgc aacaggtaca aattcaatcg gagaagcatt
     2101 taaaattgtg caacgcggtg atgcagatgc aatgattact ggtggtacag aagcaccaat
     2161 tactcatatg gcaattgctg gtttcagtgc aagtcgagcg ctttctacaa atgatgacat
     2221 tgaaacagca tgtcgtccat tccaagaagg tagagatggt tttgttatgg gtgaaggtgc
     2281 tggtatttta gtaattgaat ctttagaatc agcacaagct cgaggtgcca atatttatgc
     2341 tgagatagtt ggctatggta ctacaggtga tgcttatcat attacagcgc cagctccaga
     2401 aggtgaaggt ggttctagag caatgcaagc agctatggat gatgctggta ttgaacctaa
     2461 agatgtacaa tacttaaatg cccatggtac aagtactcct gttggtgact taaatgaagt
     2521 taaagctatt aaaaatacat ttggtgaagc agctaaacac ttaaaagtta actcaacaaa
     2581 atcaatgact ggtcacttac ttggtgcaac aggtggaatt gaagcaatct tctcagcgct
     2641 ttcaattaaa gactctaaag tcgcaccgac aattcatgcg gtaacaccag atccagaatg
     2701 tgatttggat attgttccaa atgaagcgca agaccttgat attacttatg caatgagtaa
     2761 tagcttagga ttcggtggac ataacgcagt attagtattc aagaaatttg aagcataact
     2821 ataaaaatct tcagtaacgt tgttttagtt actgaagatt ttttcagttt ctttatacta
     2881 agatgagcga cagcacaatc gtcataataa aatataaaat atttattaat aataagggga
     2941 ttatccatgt agaaacaaag taatgctctt tttttacctc ttgtgggttg aaaaatggat
     3001 catcagagat agacttcttc tttttcgaag atgacatttg atactttaat cttctaaaac
     3061 cataacttgt cgcatcaaaa atgccttctt gtacaagtaa aatcaaaaat atgctaataa
     3121 aaataattaa tgaaacataa aacaatatat ttaaatatgt aatgatagta tggctattaa
     3181 aaagccatat aataaacgtt aatattggcg ttattagtgc cattccaagc cattttttca
     3241 acatttgatc actcccactt atagaaaact cttacgcata gtttacatta aaatcagaca
     3301 ttgaggaatg attttttaat ttcttcagct ttattgaaat tctaaaatca atcattcttc
     3361 attagtttaa agcaaaaaaa tattgatata tagtaaatat tgtatatata atattagtta
     3421 agatttcaga aaattttgaa gggaatggaa atttagaaat cggaatttgt tagaggaggg
     3481 gattagatgg ggaaatatat tttcaaacga tttatttata tgcttatttc tttatttatt
     3541 attattacaa ttacattttt cttaatgaaa ttaatgccag gttcgccatt taacgatgct
     3601 aaattaaatg ctgaacaaaa agaaatttta aatgaaaaat atggattaaa tgatcctgta
     3661 gctacgcagt atttacatta tttaaaaaat gttgttacag gcgattttgg taattcattc
     3721 cagtatcata atcaacctgt gtgggatttg attaaaccga gactactacc ttcttttgaa
     3781 atgggtctta cagcaatgtt catcggtgtg atactgggac ttattttagg tgttgcagca
     3841 gctactaaac aaaattcttg ggttgactat acaactacag ttatttcagt tattgcagta
     3901 tctgtaccat cttttgtact tgctgtactt ttacaatatg tatttgcagt taaattaaga
     3961 tggttcccag tagctggatg ggaaggtttt tcgaccgcgg tattaccgtc acttgcatta
     4021 tctgcagctg ttttagcaac tgtcgccaga tacataagag cagagatgat agaggtatta
     4081 agttcagact atattttatt agcgagagct aaaggtaatt cgacaatgcg tgtacttttt
     4141 ggacatgcac ttagaaatgc tttaattcca attattacaa ttatcgttcc catgttagca
     4201 agtattttaa caggcacttt aacaattgaa aatatttttg gagttcctgg attaggggat
     4261 caattcgtac gttcaattac aacaaatgat ttctcagtaa tcatggcaat cacactatta
     4321 tttagcacac tgtttatcgt ttctattttt attgtagata ttttgtacgg tgtgatagat
     4381 ccacgaattc gtgttcaagg aggtaaaaaa taatggctga aaataaaaac aatttgtcga
     4441 ttaacgacga tcattctaat gcagctatga cgcatacctc tgacgctatc gcatcatctg
     4501 attttattat tagagaatta gatttgaatc aggaacctga aatgcaacga gaaagcaaaa
     4561 acttttggca agatgcttgg gctcagttaa aacgaaataa gttagctgtt gtcggtatga
     4621 taggtttaat tatcattgta atatttgctt ttatcggtcc agttataaat aaacatgatt
     4681 atgctgaaca aaatgtagaa catagaaatc ttccggcaaa aatacctgta ttagacaaag
     4741 ttccattttt accttttgat ggtaaagatg cagatggcaa ggatgcttat aaagcagcaa
     4801 atgctaaaga aaattattgg tttggtactg atcagttggg tcgagattta tggacaagaa
     4861 catggaaagg tgctcaaatt tcattgttta tcggtgttgt tgcagcgatg ttagatattt
     4921 ttattggtgt tgtatatggt gcgatttctg gattcttcgg tggacgtgtc gatacgatta
     4981 tgcaacgtat acttgaagtc atagcatcta ttccgaattt aattgtcgta attttatttg
     5041 tattaatttt tgaaccatcc atttggacaa ttatattggc tatgtctatc acaggctggt
     5101 taggcatgag cagagttgta cgtggagaat ttttaaaatt aaaaaatcaa gagtttgtca
     5161 tggcttcgaa aacattgggg gcttcaaaat tcaaattgat atttaagcat attttaccta
     5221 atacattagg tgctatcgtg gttacatcaa tgtttacagt acctagtgct attttcttcg
     5281 aagcattttt aagtttcatt ggtataggtg tacccgcacc tcaaacatcg ttagggtcat
     5341 tagtaaatga tgggcgcgca atgttattaa tttatccaca tgaattattt ataccagcaa
     5401 tgattttaag tttattaatt ctattctttt acttatttag tgatggatta cgtgatgcat
     5461 ttgatccgaa aatgcgtaaa taaaaagggg gcatagcata tgactgaaag aatattagaa
     5521 gtaaatgatt tgcatgtttc ctttgatatt acagcagggg aagtgcaggc agtgagaggc
     5581 gtagattttt atttgaacaa aggggaaaca ttggcaattg ttggtgaatc aggttcaggt
     5641 aaatctgtaa caacaaaagc aattacaaaa ttattccaag gggacacagg aagaattaaa
     5701 aagggagaaa ttttattttt aggggaagat ttagcaaaaa aacctgaaaa tgagttgatt
     5761 aaattacgtg gcaaagatat ttcaatgatc tttcaagatc caatgacatc tttaaaccca
     5821 acgatgcaaa ttggtaaaca agtcatggaa ccattaatta agcacaaaaa ttatagtaaa
     5881 gcacaagcta aaaagcgcgc attggaaata ctaaatcttg taggtttacc aaatgcagaa
     5941 aaaagattta aagcatatcc tcatcaattt tcaggtggac aaaggcaaag aattgttatt
     6001 gcaaccgcat tagcttgtga acctaaagtg ctcattgctg atgaaccaac gactgcatta
     6061 gacgtaacga tgcaggcaca aattttagat ttaatgaaag aactacaaca aaaaatcgat
     6121 acagcaatta tttttataac gcatgattta ggggttgttg cgaatattgc tgatagagtg
     6181 gcagttatgt atggtggtca aatggttgaa acaggagatg ttaacgaaat attttatgat
     6241 ccaaagcatc catatacatg gggattatta tcgtcaatgc ctgatttatc aacaacaaat
     6301 gacacaccat tactagcgat tcctggagcg ccacctgatt tattacaccc acctaaaggt
     6361 gatgcatttg cgagacgtag tcaatatgca ttagatattg attttaaagt agaaccaccg
     6421 tggtttaaag tttcaccgac acattttgtg aaatcttggt tattagacgc acgtgcacca
     6481 aaagttgaac tacccgagct ggtaaaacaa cgtatgaaac cgatgcctaa taattatgaa
     6541 aaaccactca aggtagaaag ggtgtcgttc aatgaaaaat gatgaagtgc tattatctat
     6601 taaaaattta aagcaatatt ttaacgcagg aaagaaaaac gaagtgagag cgattgaaaa
     6661 tatttcgttt gatatataca aaggggaaac attaggttta gtaggagaat cggggtgtgg
     6721 taaatctaca actggtaaat caattattaa acttaatgat attacaagtg gagaaatttt
     6781 gtatgagggt attgatatac aaaagattcg taaacgtaaa gatttgctta aatttaataa
     6841 aaagatacag atgatttttc aagacccata tgcgtcttta aatcctaggt taaaagtaat
     6901 ggatatagta gctgaaggta ttgatatcca tcatttagca actgataagc gtgaccgaaa
     6961 aaaacgtgtc tatgatttac ttgaaactgt tggattaagt aaagaacatg ccaatcgcta
     7021 tcctcatgaa ttttcaggtg gacaacgcca acgtattgga attgcccgtg cattagccgt
     7081 tgaaccagaa ttcattatcg cggacgaacc aatatcggca ttggatgttt caatccaagc
     7141 tcaagtagtt aatttattat taaaattaca acgtgaaaga gggattacgt tcctatttat
     7201 agctcatgat ctatcaatgg tgaagtatat ttcagatcgt attgcagtca tgcattttgg
     7261 gaaaatagtt gaaattggac cggcagaaga aatttatcaa aatccattac acgattatac
     7321 taagtcttta ttatcagcca ttccacaacc tgatcctgaa tcagaacgca gtcgcaaacg
     7381 atttagttat attgatgatg aagcaaataa tcatttaaga caattacatg aaattagacc
     7441 gaatcacttt gtctttagta ctgaagaaga agcggcacaa ctacgagaaa ataaattggt
     7501 gacacaaaat taaggggaag ggggaaatgc aatgacgaga aaatttagaa cacttatttt
     7561 aattttgatt gctacaattg cattaagtgg ttgtgctaat gacgatggta tttattcaga
     7621 taaaggtcaa gtattcagaa aaattttgtc atcagactta acatcccttg atacatcatt
     7681 aataacggat gaaatatctt ctgaagtgac tgcgcaaaca ttcgaaggtt tatacacatt
     7741 aggaaaaggt gacaaaccgg tgttaggtgt tgcgaaagct tttcctgaaa agagtaaaga
     7801 tggtaaaact ttaaaggtta aattaagaag cgatgctaaa tggagcaatg gtgacaaagt
     7861 gactgcacaa gactttgttt atgcttggag aaaaacagtt gaccctaaaa caggttctga
     7921 atttgcatac attatggggg acattaaaaa tgcgagtgat attagtactg gtaagaaacc
     7981 tgtagagcaa ttaggtatca aagcattaaa tgatgaaaca ttacaaattg aattagaaaa
     8041 gccggttcca tatattaatc aattattagc acttaatacc tttgcacctc aaaatgaaaa
     8101 agttgccaaa aaatatggta aaaattacgg tacggcagct gatagagcgg tatacaatgg
     8161 tccatttaaa gttgatgatt ggaaacaaga agataaaacc ttactatcta aaaatcagta
     8221 ttattgggat aaaaagaatg taaaattaga taaagtgaat tataaagtta ttaaagactt
     8281 acaagccggt gcatcattgt atgatactga atcagtagat gacgcagtta ttactgcaga
     8341 tcaagtaaat aaatataaag acaacaaagg attaaacttt gtgttaacga ctgggacatt
     8401 ttttgtaaaa atgaatgaaa aacaatatcc tgattttaaa aacaaaaatt taagattggc
     8461 tatcgcacaa gcaatagata aaaaaggata cgttgattca gtgaaaaaca atggctcaat
     8521 tccttccgat acactaacag ccaaaggaat tgcgaaagcg cctaatggca aagattatgc
     8581 gagtaccatg aattcgcctt taaaatataa tcctaaagaa gcaagagcgc actgggacaa
     8641 agctaaaaaa gagttaggta aaaatgaagt gacattttca atgaacacag aagatacacc
     8701 agatgcaaaa atatctgctg aatatatcaa atcgcaagtt gagaaaaatt taccaggagt
     8761 tactttgaaa attaagcaat taccgtttaa acaaagagta tcactagaac tgagtaacaa
     8821 ttttgaagca tcacttagtg gttggtctgc agattaccct gatcctatgg cttatttaga
     8881 aacaatgacc acaggtagcg cacaaaataa tacagactgg ggtaataaag aatatgatca
     8941 attacttaaa gtagcaagaa ccaaattggc acttcaaccg aacgaacgat atgaaaactt
     9001 gaaaaaagca gaagaaatgt tcctaggaga tgcaccggta gcaccaattt atcaaaaagg
     9061 tgttgcacat ttaacaaatc ctcaagtaaa aggattaatt taccataaat ttggtccaaa
     9121 taactcactt aaacatgtat atattgataa atcgatagat aaagaaacag gtaagaagaa
     9181 aaaataatat gctttgtaaa ttaggctgga gacatatctc cagtcttttt gtgttggata
     9241 aaaactttgg gaataaaaat ttaaaataag tcgtttttta aattactgaa attgattaaa
     9301 tgcataaata actgaatatt ctaaaaataa acttgtaata attttttcta tgagtaaact
     9361 aaaaagaaaa aattagattg aaagtaggag gcatatgtat ggggaagcta attaaatata
     9421 tttcaatact tcttattgtc gttttagtgt tgagtgcttg cggaaaaagc agtaataaag
     9481 atgaaggagt aaaagatgct actaaaacgg aaacctcaaa acataaaggt ggtaccttaa
     9541 atgtagcatt aacagcaccg ccaagtggtg tttattcttc gttattaaat agtacacatg
     9601 cagattctgt agttgaggga tattttaacg aaagcttatt agcaactgat aaaaaaatac
     9661 gtcctaaggc atatattgct tcatggaagg acatcgagcc ggctaagaaa atagaattta
     9721 aaattaaaaa aggtattaaa tggcatgatg gtaatgaatt gaaaattgat gattggattt
     9781 attcaattga agtcttagct aacaaggact acgaaggtgc ttattatcca agtgtagaaa
     9841 atatccaagg tgcgaaagat tatcatgaag gaaaaactga tcatattagc ggattgaaga
     9901 aaatagatga ctacactatg caggttacat ttgataaaaa acaagaaaat tacttaacag
     9961 gatttattac tggaccttta ttaagtaaaa aatatttatc agatgtacca attaaagatt
    10021 tagcgaaatc agataaaatc cgaaaatatc ctattggtat tggaccgtat aaagttaaga
    10081 aaatcgttcc aggtgaggct gttcaactcg ttaaatttga tgattattgg caaggtaagc
    10141 ctgcactaga caaaatcaat ttaaaagtta ttgatcaagc gcaaattatt aaggcaatgg
    10201 aaaaaggcga tattgatgtt gcgaatgatg ctaccggtgc aatggcaaaa gatgctaagt
    10261 catctaatgc tggtctcaag gtattatctg cgccaagctt agactacggt ttaataggat
    10321 tcgtatctca tgattacgat aaaaaagcta ataaaactgg taaagtgaga ccaaaatatg
    10381 aagacaaaga attacgtaaa gcaatgcttt atgcaattga tagagaaaaa tggatcaaag
    10441 cgtttttcaa tggttacgct agtgaaatca atagttttgt accatctatg cattggatag
    10501 cagccaatcc taaggaccta aatgattaca aatatgatcc tgaaaaagct aaaaaaatct
    10561 tagataagtt aggttataaa gatagagatg gtgacggatt tagagaagat cctaaaggta
    10621 ataaatttga gattaacttt aaacattatt caggttctaa tcctactttt gaaccaagaa
    10681 ctgctgcgat aaaagatttc tgggaaaaag ttggcttgaa aacaaatgtg aagttagtag
    10741 aattcggtaa atataatgaa gacttagcaa atgcatctaa agatatggaa gtgtacttca
    10801 gatcatgggc aggaggtaca gatccagatc catcagattt ataccacact gatagacctc
    10861 aaaatgaaat gagaacagtt ttaccaaaat cagatcaata tttagatgat gcattagact
    10921 tcgaaaaagt aggcattgat gaaaagaaac gtaaagatat ttatgttaaa tggcaaaaat
    10981 atatgaatga tgagttacct ggattaccaa tgttccaagg taaatcgata actattgtta
    11041 acgataaagt acgaaactta gacattgaaa ttggaactga tcaaagttta tataatttaa
    11101 ctaaagaagc ttagtaatgt aacctcgacg attttgtcga ggtttactta tatgggggga
    11161 tgttatgaat aatgtattgt tagaggttaa agatttagaa acatcattaa aaataaataa
    11221 tgaatggtta gcaactgttg aaaatatttc ttttgaatta tctaaaggag aagttttggg
//
LOCUS       DAKTIP010000076        11120 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAKTIP010000076 DAKTIP010000000
VERSION     DAKTIP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.076"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            126..842
                     /locus_tag="O0305_001464"
     CDS             126..842
                     /locus_tag="O0305_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0274612.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            842..1315
                     /locus_tag="O0305_001465"
     CDS             842..1315
                     /locus_tag="O0305_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCY0274613.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDGRRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1407..1523
                     /locus_tag="O0305_001466"
     CDS             1407..1523
                     /locus_tag="O0305_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274614.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1668..2312
                     /locus_tag="O0305_001467"
     CDS             1668..2312
                     /locus_tag="O0305_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0274615.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2428..3294
                     /locus_tag="O0305_001468"
     CDS             2428..3294
                     /locus_tag="O0305_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0274616.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKEWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3564..4784
                     /locus_tag="O0305_001469"
     CDS             3564..4784
                     /locus_tag="O0305_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCY0274617.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4781..5269
                     /locus_tag="O0305_001470"
     CDS             4781..5269
                     /locus_tag="O0305_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCY0274618.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5368..6057)
                     /locus_tag="O0305_001471"
     CDS             complement(5368..6057)
                     /locus_tag="O0305_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCY0274619.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6184..6627
                     /locus_tag="O0305_001472"
     CDS             6184..6627
                     /locus_tag="O0305_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0274620.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISNTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6694..7089
                     /locus_tag="O0305_001473"
     CDS             6694..7089
                     /locus_tag="O0305_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274621.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKDKEASQDKQSK
                     DQTKSSDKDNHKKPTSADKDQKANDKHQS"
     gene            7227..7526
                     /locus_tag="O0305_001474"
     CDS             7227..7526
                     /locus_tag="O0305_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274622.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGHTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7606..8148
                     /locus_tag="O0305_001475"
     CDS             7606..8148
                     /locus_tag="O0305_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0274623.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEEHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8244..8810)
                     /locus_tag="O0305_001476"
     CDS             complement(8244..8810)
                     /locus_tag="O0305_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCY0274624.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEKFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8812..9270)
                     /locus_tag="O0305_001477"
     CDS             complement(8812..9270)
                     /locus_tag="O0305_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCY0274625.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9273..9956)
                     /locus_tag="O0305_001478"
     CDS             complement(9273..9956)
                     /locus_tag="O0305_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274626.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10128..11003)
                     /locus_tag="O0305_001479"
     CDS             complement(10128..11003)
                     /locus_tag="O0305_001479"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCY0274627.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3905 a         1569 c         1923 g         3723 t
ORIGIN      
        1 aaaactaagt aacagtttga agaaatcgta gttcaataat gttaattgtg aaaatgtata
       61 taaacataaa aaaatcatgt ataatatatg ttgttaatta aacagttcga aagcgagatg
      121 acattatggg acgtaaatgg aataacatta aagaaaaaaa ggcccaaaaa gataaaaaca
      181 caagtagaat atatgcgaaa tttggtaagg agatttatgt tgcagcaaaa tctggtgaac
      241 ccaatccaga atctaaccaa gctttaaggt tggtgcttga acgcgctaag acatattcag
      301 tgccgaatca tattattgaa aaagcaatag ataaagctaa gggtgctgga gacgaaaact
      361 ttgatcacct aagatatgaa ggatttggcc caagcggatc aatgctaatt gttgatgcgt
      421 taacaaataa tgtaaatcgc actgcctctg atgtgcgagc tgcttttggt aaaaacggcg
      481 gtaatatggg tgtatctgga tcagttgctt atatgtttga tcatgtggca acatttggta
      541 ttgaaggaaa gtctgttgac gaaatacttg aaacattaat ggaacaagat gtagatgtaa
      601 atgatgtgat tgacgataat ggattgacaa tagtctatgc tgaaccagat caatttgcag
      661 tcgttcaaga tgcgcttcgt gcagcaggtg ttgaagaatt taaagttgct gaatttgaaa
      721 tgttacctca aacagatatt gaactttctg aagcggacca agtaacattt gaaaaattaa
      781 tcgatgcatt agaagattta gaagatgtac aaaacgtatt ccataatgtg gatttgaaat
      841 aatgaaatca gcagaacaat ggattgatga attgcaactt gaatcacatc ctgaaggtgg
      901 tttctataga gagacaattc gagaagtatt gaaagatgga cgcagagcgc cgtttagtag
      961 tatttatttt ttacttacag atgacaatat ttcgcatttt catcgaattg atgctgatga
     1021 agtatggtac tatcatgctg gcgattctct aacaattcat atgataaatc cggatgggga
     1081 atatacgact gcaacattgg gtactgatat ccaaaatgga gatgtattgc aatatgtagt
     1141 gcctaaagga acaatttttg cttcttcaat cgaaatatca aatactttta gtttagtagg
     1201 ttgtatgtgt caaccggcat ttgagtttaa gcagtttgaa ttgtttaagc aatctgaatt
     1261 aattacgcaa tatccgcatc ttaaatcagt aatcgaaaaa tatgctttaa aataaaagtg
     1321 atcaatgaag tggtttgaag gttgttaata aacctttgag tcacttcatt tttatatgta
     1381 ttcttgattg aatcagaata gatttgatgc ttcagctgtt tttaatgaaa atagcattaa
     1441 atgattttga aaacgataag agtgtgttat ttatattttt gaaaaatcac ttttatgaag
     1501 aaatgtgtgt actatctaat taatattgtc tattttaagt aaattgaagc gagttgtagt
     1561 aaaataataa taaatatttt catatgatta acaaaaacta taaaactgta tcatgacatg
     1621 acatcattta tgtagtttta tataataatg acaaggagtt ggtgactgtg aaagtaaagt
     1681 atatagataa acgtcactgg cgtcgcctaa ttgataggga atacacagag gtaaaagtta
     1741 ataataatag gtttaagggt attataggct tagtcacgat gaaaaaggtt cgtgatcctt
     1801 tagaggtgac ggtagttgga caaaatatca ttgtcgcaga tgacaattat aaatggttgc
     1861 aaatactacc tgaaaagaaa cgttatagta taactgtaat gtttgataat aaaggcaatc
     1921 cattagaata ttattttgat ataaatatca aaaatataac gcaaaaaggt aatgcgcgta
     1981 cagtagattt atgtttagat gttttagcat taccaagtgg tgaatatgag ttggtagatg
     2041 aagatgattt aatgtttgca ttagaaagtg agcaaattac aaaaaagcaa tttcatgaag
     2101 catatatgat tgcacatcaa attatggcag agttagaaaa tgattttaaa ggattccaaa
     2161 agaaaatcat gtactgcttt aataaaatta atgcaaaggc tcaaaaaaat catcaaaagc
     2221 cacaaaataa aactaatatt gaaaaaagca aacaaataaa gcctaagcaa tataatcaaa
     2281 ctaaaaatca ccaacaacaa aagaaaaact aagtaattca agctgcagcc ataccaataa
     2341 aattggtgaa attattcgtt agtaatgaac tcatgaatgc ttgtagccat gaaagttcaa
     2401 taattgaata atttatgggg ggaaattatg aagattgaag actatcgttt actaataaca
     2461 ttagacgaaa cgaaaacgtt acgtaaagcg gctgaaattt tatatatatc tcaacctgct
     2521 gttacacaaa gactaaaagc tattgaaaat gcttttggag tagatatttt tatcagaaca
     2581 aaaaaacaat tgattacaac aactgaagga acaatgatta ttgagcatgc tcgtgacatg
     2641 ttgaaaagag agcgattatt ttttgacaaa atgcaggcac atattggtga agtgaatgga
     2701 acaatatcaa tcgggtgttc ttctttgatt ggacaaacct tacttcctga agttttgagc
     2761 ctatataatg cccaatttcc taatgttgaa atacaagtgc aagttggttc aactgaacaa
     2821 attaaagcaa atcatagaga ttatcatgtt atgataactc gtggaaataa ggtaatgaat
     2881 ttagctaaca cacatttatt taatgatgat cattatttta tttttccaaa aaatagacga
     2941 gatgatgtta caaagttacc atttatagag tttcaagctg atccgattta tataaatcaa
     3001 ataaaagaat ggtataacga taatttagaa caagattacc atgcaactat tacagtggat
     3061 caagtagcaa cttgcaaaga aatgttgatt agtggtgtag gtgttacaat cttgccagaa
     3121 attatgatga aaaatatcag caaagaacaa tttgagtttg aaaaagtaga aattgataat
     3181 gaaccgctga ttcgttcgac atttatgagt tatgatccga gcatgttgca attgccacaa
     3241 gttgattctt ttgtaaatct catggcgagc tttgttgaac aaccaaaggc gtagttttag
     3301 actaatttaa ggtttgtatt taattttaaa ctattcggtt aaattgaacg tagttggttg
     3361 ctaatgcacc aacagcaaaa gagcccctaa ttaataaatt aaaaggggac aaaggaatac
     3421 agttggttgc taatgcacca actgcataag agcccctaat taataaatta aaaggggaca
     3481 aaggaataca gttggatgct aacgcaccaa ctgcataaga gcccctaatt aataaattaa
     3541 aaggggctct aagaagcggg gttatggata tgtttgcagc gttattacaa ataaagaatt
     3601 ataaactctt tgttgctaat atgtttctac taggtatggg tattgcggtt acggtcccat
     3661 atcttgttct ttttgcaact aaagatttag gtatgacaac aaatcagtat ggattacttc
     3721 tagcatctgc agcgattagc cagtttacag taaattcaat tattgctaga ttttcggata
     3781 cgcatcactt taatagaaaa attattatta ttctcgcatt attaatgggt gcgcttggtt
     3841 tttcaatata cttttttgta gatacaatct ggttattcat attactatat gcgattttcc
     3901 aaggattatt tgcaccagca atgccccaac tttacgcatc tgctagagaa tctatcaatg
     3961 tttcaagctc taaagataga gctcaatttg ccaacacagt attacgttca atgttctcat
     4021 tgggcttttt atttggtcca tttattggtg cccaattaat cggattaaaa ggctatgctg
     4081 gattgtttgg tggaacaata agtatcattt tatttacttt agtacttcaa gtgtttttct
     4141 ataaggattt aaacattaaa caccctatta gtacgcaaca acatgttgaa aaaattgctc
     4201 ctaatatgtt taaagacaaa acgcttttat taccatttat tgcatttatt ttattacaca
     4261 ttggacaatg gatgtatacg atgaatatgc ctttatttgt tactgattat ttaaaagaaa
     4321 atgaacaaca tgtcggttat ttagctagtt tatgtgctgg tttagaagtg ccatttatga
     4381 tcattcttgg cgttttatca tctagattac agactcgaac attgttgatt tatggagcga
     4441 tttttggtgg tttattctac ttcagcattg gggtatttaa aaacttctat atgatgttag
     4501 caggacaggt gtttttagct attttcttag cggttctttt aggaattggt attagttatt
     4561 tccaagatat cttaccagat tttccaggat acgcttcaac actattttct aatgcaatgg
     4621 ttattggaca gttaggcggt aacctattag gtggtgctat gagtcactgg gtaggtttgg
     4681 aaaatgtatt ttttgtatca gcagcatcaa tcatgttagg tatgatactt atattcttta
     4741 ctaaaaatca aaaaattaca aaagaggatg tgatatcaac atgacaatta ttttatggct
     4801 acttatcatc gctgccttca tgttagcatt tgttgggttg attaagccga ttattccttc
     4861 tgttttagta ttatgggttg gctttttaat ctatcaattt ggctttcata atcagcattt
     4921 atcatgggtg ttttatgtat ctatggcatt gctaacaata ttaattttat gtgccgactt
     4981 tttagctaat aaatattttg tgaatcgctt cggtggttct aagtttggag agtatgcagc
     5041 tttaattggt gtggttattg gatgttttgt tttaccgcca tttggaatta ttattatacc
     5101 ttttattttg gtattcatag ttgaattaat acaaggctat tcatttgaaa gagcagttaa
     5161 agtaagtata ggttcaatcg tagcattttt aacaagtagt atagctcaag caatcattat
     5221 gtttataatg attgtatggt tctttataga tgctttattg attaattaat aaaaagctta
     5281 ttgcaaaata tgtttttcgg taactgtaat ttagtgattt tatcattaac agtaccaaat
     5341 tcgcatatga tgtaataagc tttttgttta taaaaatgta tgagaatgtt ttttcgaaat
     5401 atttctttca atgcgtaatc caataggcat aactataaat gaaaatataa taaataccca
     5461 attgcttacc tgaatatatg gtccaaaggc aagtaatgat tgtggaataa agaatacata
     5521 gaaaaatcct acaattagta gaatgattgc taaataggtg aatatccata tccaatttga
     5581 attgttaaaa cactgcagtt gtattgtttt aaaactaaac catataaata aaaagacaat
     5641 aaagaatcca acgactactg aaaacgggaa tgaaacaaaa tataaattac ttccattttt
     5701 ttccatgaaa aatcctaaaa atcctttgag aaaactaaca atcccaatta atagaacgat
     5761 gtgttgatag atatatttaa aaatattttt aaatgtttca ttaggcatcg cttttagttc
     5821 ttttattgca tgtgcttttg ggtcgtgatt gaaaaaatct aaggctaata aaccatgttg
     5881 ttctgcgctt aataattgtt tgagtatacg gttaataatt aactctgtat catgagggtt
     5941 gacgcgaaag tcagagcgca tataagtcat ataattctcg aagatttctc tatcagtatt
     6001 gcttaatctt aatgatttaa cattattttc ttttgttaat tgcgcagtac ttttcattgt
     6061 tacttaagcg ctcctttaaa aatgtttaat tccaaattaa aatggaaatg attttatagt
     6121 attaataagg tcaatcatat catattaaac gcataaatat aacgattaat attggagagg
     6181 aaaatgagga cacttaataa agatgaacat aattatatca agcaaatagc taatatacat
     6241 gagacattat tgtcgcaagt agaatccaac tataaatgta ctaaactgag tattgctctt
     6301 aggtacgaga tgatatgttc aagattagaa catacaaatg ataaaattta tatatatgaa
     6361 aatgaaggtc aattaatagc gtttatttgg ggacatttta gtaatgaaaa aagtatggtt
     6421 aacattgaac tgctatatgt tgaaccacaa tttcgcaaac tgggaatagc tacgcaactg
     6481 aagattgcgc ttgaaaaatg ggcaaaaact atgaatgcaa agcgaataag caatacaatt
     6541 cataaaaata atttgccaat gatatctttg aataaagatt taggttatca agtgagtcat
     6601 gtgaaaatgt ataaagatat tgattagaat taggattatg ttgctaattc atgttaaaat
     6661 taaaaaagat ttaatgacgt taaggagttt tatatgaaga aattaatcat cagtattatg
     6721 gcggtcatgc tatttttaac aggttgtggt aaaagtcaag agaaagccac tctggaaaag
     6781 gatatcgata atttacaaaa agaaaataaa gaattaaaag acaaaaaaga aaagcttcaa
     6841 caagaaaaag aaaaattagc agataagcaa aaagaccttg aaaaagaagt gaaagattta
     6901 aaaccttcaa aagaagataa caaggatgat aaaaaagacg aagacaaaaa taaagacaaa
     6961 gataaagata aagaggcatc acaagataag caatcaaaag atcaaactaa gtcatcggat
     7021 aaagataatc acaaaaagcc tacatcagca gataaagatc aaaaagctaa tgacaaacac
     7081 caatcataat cgaattgctt acttgttata gatgaaaggt acagcgtttt aaaccttatt
     7141 ttaagggtat gtattaatta aaatgtggtc atgattgaaa cagaacgtaa aaatagacaa
     7201 cgtaattaat aaaggagaga aacggcatgc atgaacaaga ttttagaatt ttagagggtc
     7261 aagatattac tttgccagaa ttaggtagag aattagaaaa tattacagga catacgattg
     7321 ctgattctac tggcgaaatt aagcgtgtaa ttgcacattt accaaacttt gagtccgata
     7381 cagatacttt tgttgctaca tatcgtttaa accatcaaca agattttata gatgcaactt
     7441 ttactgcgct gaaatcagat agagcacgtt taaaagaagt gccagttcat gttgaactta
     7501 taagttatat ttctaaatca aaataaactg ctatctaaaa cgcaaagttg atcaaaatat
     7561 cgattttgtg ttttttattg agaaattata taggagtgtc aatcgatgat ttattgtgaa
     7621 acagagcgtt taatattaag agactggcat gaagatgatc tgttaccttt tcaaaaaatg
     7681 aatgcgaatt atgacgtacg taaatatttt ccaagtttat tgagttatcg tcgttcagaa
     7741 ttagatatga gaactatgga tgcggttatt aaagattatg gcattggatt atttgctgta
     7801 gaagataaag agtcccatca atggataggc tttataggtt tgaattatat tccagaaaca
     7861 agcgattatc catttaaaga attaccgctt tatgaaatag gttggcgctt gttgccagaa
     7921 ttttggggaa aaggattagc aactgaaggc gcaaaggcaa cattgaagtt agcagaagaa
     7981 catcaaatat acgatgtcta tagttttaca gcagaagcaa ataaagcttc acaacgtgta
     8041 atggaaaaaa ttggcatgac agtgtatgat catttcgaat tacccaatct aagtaagtat
     8101 catttattaa aaaggcaagt gcgctattac attaatcttc gaaagtgaaa aatttataca
     8161 taagcgtaac aaacacccct aacattgttt agctgatgat aacaatatta ggggtgtttg
     8221 tttatttttt aaccttagaa tgattaatcg tatgaacgag tacccagagg tttgaaattt
     8281 aatattgatt caattaatga ttccttagtg tcgcataacg gtgcaagagc acgatactta
     8341 ggatcaataa aaccttcctc aatcatatgg tcaatcattg tttgtagtgg attgaaaaag
     8401 ccattaatat tataaatggc aataggcttt tcatggatac ctatttgagc ccaactatac
     8461 atttcgaaaa atttttctag tgaacctgcg ccaccaggag ccatgacaaa tgcatctgca
     8521 agttctgcca ttttattttt acgttcatgc atagaatcaa ctaaaattaa ttcagttaaa
     8581 cgttggcttg tgatttcatg ttcatctaac attttaggca tgacgccaat agctttgccg
     8641 ccatgatcta atacaccatc ttgaatggca cccataatgc caattgaccc tgcaccaaat
     8701 actaattcat aaccttgttc agcaaaatat ttacctaaat cgtatgcttt ttgtacatat
     8761 gaagggtcat gacctttgct tgcaccacaa taaactgcga ttcgtttcat gttaatccag
     8821 ctccttaatt cgatgaatga cttttaatag tgattgttca aacacttttt gatcttgctt
     8881 tgtaaaaggt gggggacctt tgtggcgacc accttgtttt ctaatttgtg cattcatata
     8941 tcgtttatct aatagttgtt gaatattttt ggaattgtat atcttcccat tatgatgcat
     9001 gacaattaag actttgtcga ctaataaact tgcgagtcca taatcttgag tgactacgat
     9061 atcatccttc gttgataatt gaacaatttt gtaatcaact gcatctggtc catcatcaac
     9121 atataatgtt gatacatgtg gaggatataa ttggttcgaa aaatggctga agctccgaat
     9181 aattgtcaca aaaatgcctg tctcagttgt taaatctata atagaatcaa caacaggaca
     9241 agcatctcca tcaataataa tatgtgtcac aattatgcct ctgtattgtt ttctttattt
     9301 tgttgagagg cgcttttggc aacataatct ttatattttt taaatgactt gatgcgtgct
     9361 ttatcagctt cttgttggcg tttttgttct tctttgtgtc gtttttcaat atttttttgt
     9421 aactttttat tcattttagc gatttctttg cgattttttt cagctagttt atcgcctttt
     9481 ttctcagttt tctcatctaa tttattaggt gttaagcctg ctttttcttc gtatttttgt
     9541 gattttttca tatctttaat acgttgtatt tcattctttt cgcgggcttt ttgctcttct
     9601 ttatgacgct tttcgatatt tttttgaagt attttattca ttttatcagc gtctttacga
     9661 ttttgtttag ctaatttttc gccttttttc tcaatatagg caggatcatg ttctctagca
     9721 aactttttaa gttcacgttt attttcaaaa tcttgttttt tatcgccgac atattcttta
     9781 acatcactcg ctgtgttact gattgctgca gatgtttttg aagcaacttt acttgtagca
     9841 tctgtaactt tttgtacgtc cggatgttgt ttgatacgtt tacgttcaac aattaacggt
     9901 accaatacaa ttggtaatac attaatcata aatttgatga cttttttctt atccatagat
     9961 cttgcctcca taattacttt attaatttta cataccctat gatacatcaa tataaacgat
    10021 gatagtagtg aatcactatt aagtatttca gatgtttttt aaaagaagac aataaaaact
    10081 gccaatcaag tgattcctta attgacagtc tatattttaa aaggaaatta aataccttta
    10141 ccaatgccaa atccgaagta aagtatagca ataaagatta ctaatacaat tctgtaaatg
    10201 gcaaatggaa ttagtttgat tttgttaatt agatgcaaga atgttttgat tgcaattagt
    10261 ccaacagtaa atgcagctaa aaagcctaaa atataaaaag gtatatcagc aatctgaata
    10321 tcttgataat gttttaataa agataaacca ctagctgcta acataattgg aacagccata
    10381 ataaatgtaa agtccgatgc tgctttatga tttaatttca ttaatacccc agttgaaatt
    10441 gttgagcctg aacggctgaa accaggccac atagctactg cttgagaaat accaattaca
    10501 aatgcttgga aataactgat ttgatctact gtttgtgggt ttttaacttt agctgagtat
    10561 ttatcagcaa taatcatata gatagcacct acgaataagc caatcataac agttggcaca
    10621 ctaaataaat gttcttcgat gaaatcatca aatagtaagc ctaaaatacc tgctggcacc
    10681 atacccacta atacatgtaa taaatttaaa cgtcttggct ttgaacgtct ttgttgatcg
    10741 ttatctcctt caacatgttt gtgtttacca atatgtaaaa tctctaagaa gcgttcgcgg
    10801 aacacccatg ctgctgcaaa gacggatcct aattggatga cgattttaaa tgtaaatgct
    10861 gactgagaac ctaaaaattc agatgatttt aaccacatat catcaactag gatcatatgt
    10921 ccagtagagg aaacaggtgc aaattctgtt aatccttcga cgacccctaa gataatacct
    10981 tttattaatt caatgataaa cataatgtac ccactttcat tactcaattt aatttattta
    11041 aatatcaaaa ttaccatatc atgatagcat attcatttaa agacatgcta gttataatac
    11101 tagactaaag atgtatatat
//
LOCUS       DAKTIP010000077        11093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.077, whole genome shotgun sequence.
ACCESSION   DAKTIP010000077 DAKTIP010000000
VERSION     DAKTIP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.077"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            53..1042
                     /gene="ccpA"
                     /locus_tag="O0305_001480"
     CDS             53..1042
                     /gene="ccpA"
                     /locus_tag="O0305_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCY0274628.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(1140..2309)
                     /locus_tag="O0305_001481"
     CDS             complement(1140..2309)
                     /locus_tag="O0305_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCY0274629.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(2334..2966)
                     /locus_tag="O0305_001482"
     CDS             complement(2334..2966)
                     /locus_tag="O0305_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0274630.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTHEDLKALTFDAHLTAFRD
                     AEDQYKALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGKLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFENYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            3135..4841
                     /gene="acsA"
                     /locus_tag="O0305_001483"
     CDS             3135..4841
                     /gene="acsA"
                     /locus_tag="O0305_001483"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCY0274631.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRIFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            5281..6948
                     /locus_tag="O0305_001484"
     CDS             5281..6948
                     /locus_tag="O0305_001484"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCY0274632.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            7244..9931
                     /gene="harA"
                     /locus_tag="O0305_001485"
     CDS             7244..9931
                     /gene="harA"
                     /locus_tag="O0305_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCY0274633.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            10062..10154
                     /locus_tag="O0305_001486"
     CDS             10062..10154
                     /locus_tag="O0305_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274634.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(10134..11039)
                     /locus_tag="O0305_001487"
     CDS             complement(10134..11039)
                     /locus_tag="O0305_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0274635.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
BASE COUNT         4000 a         1640 c         2072 g         3381 t
ORIGIN      
        1 tgaaatgaaa acatgttatt ataatgaata aaacgtttac aaggaggaaa ttatgacagt
       61 tactatatat gatgtagcaa gagaagcgcg tgtctctatg gccacagtgt cgcgtgttgt
      121 taatgggaac caaaatgtta aagcagaaac taaaaataaa gttaacgaag tcattaagcg
      181 tttgaattat cgtccaaatg ctgttgctag aggtttagct agtaaaaaga caacaacagt
      241 aggtgtgatc attccagata tatctaatat ctattattca caacttgctc gtggacttga
      301 agatattgca acaatgtata aatatcactc aattatttca aattcagata acgatcctga
      361 aaaggaaaaa gaaattttta ataacttatt aagtaaacag gttgatggta ttattttcct
      421 tggtggtaca attactgaag aaatgaaaga attgataaat caatcatctg tacctgtagt
      481 agtatcagga acaaatggta aggatgcaca tatagcatca gttaatattg attttactga
      541 agctgcgaaa gaaattacgg gagaattaat tgaaaaaggc gctaaatcat ttgctttagt
      601 aggtggagaa cattctaaaa aagctcaaga agatgtttta gaaggtttaa ctgaagtgtt
      661 aaataaaaat ggccttcaat taggtgatac attgaattgt tctggtgctg aaagttataa
      721 agaaggcgta aaagcttttg ccaaaatgaa aggcaatttg ccagatgcca ttttatgtat
      781 cagcgacgaa gaagcaattg gtattatgca tagtgcaatg gatgctggta ttaaagttcc
      841 agaggaatta caaattatta gtttcaataa tacacgatta gttgagatgg ttagaccaca
      901 actttctagt gttattcaac cattatatga tatcggtgca gtagggatgc gcttattaac
      961 aaaatatatg aacgatgaaa agatagaaga accaaatgta gttttacctc acagaattga
     1021 ataccgagga actacaaaat aaattcacaa aattaggcat tcatctaacg acccaaatta
     1081 tatgggtgtt ggaagaatgc cttttattta tcttttaaaa tcgttgcaga ttaggttact
     1141 tattgacgag tagattcgta ccaactcgct atatgtaaag ctaatttttt atttttttca
     1201 ctaatttctt ttgtgcgggg gacataggta taatcattta aacgatcttc ccatctttta
     1261 ggtaataatt cagatgaata atgtttccat ttattaatcc attctaacgg taaataacca
     1321 ctttgaattg gttgatcaat taaacttaag aatacatgac tccatgcacg tggtacgact
     1381 ctccaaatat tgtagcctcc gccaccaaac ataattacct ttccattcgt ataagaatca
     1441 gctaaatatt ttacaaaata tggaatttca tataatgaat gtaacgtaca atttagatga
     1501 gttagtggat cacgataatg tatatcgaca ccatttacgc ttagaataat atcaggttta
     1561 aaactcttta cgacaggctc aactgttaat ttaaaacact ccaaaaatga tgcatcttct
     1621 gtatacggtt caagtgggac atttacagtg tgtccatagc cgatatcttc accgcgctca
     1681 gtatagtgac cagagcctgg gaaaagaaat tttccggttt catggataga ataagtagta
     1741 acatggttat cggcatagaa actccattgt gtaccatctc catgatgtgc atcggtatct
     1801 atgattaaaa cgcgttgatt gtattcttta gctaagtatt gtgcggtaat tgcaatatca
     1861 ttgtatatac aaaaaccact tgctcgacca ggttgagcgt gatgcaaacc accacctaag
     1921 tgacaaccat ttaatacttt gcctgacata ataagatctg ctaaagttaa agcgcctcca
     1981 acaattgtgg cactatggcg gtgcatatgc ttaaattgac cattctcttc atcatttaat
     2041 ccatatttct tagcctcatc ttcactgata atgccatgtg aagcatgctt aatagcttcg
     2101 acgtaatcat atttatgaat taacattaat tcgtcatctg ttgcaattct aggttgtact
     2161 atttgttctg gagacaataa atttgcattc aaaagtagct ctgttgttaa ttttaaacgc
     2221 atttgattga agggatgttg gtcatgaaat cgatattgta ataacttatc tgaataaaca
     2281 tatgcagttt ttgatgaatg ttgttgcata taatccctcc gatattccaa aaattaaaag
     2341 aaaaaccgat tcatataacg aatatcatca aacgcttgtt gctgttctaa tgtaatgttt
     2401 ttgccaattc ttgccattaa acaattagct ggatgacttg ttatttctgg atcatctgta
     2461 gcgaatattt caagtccacc agttgccatt aaccgctgca ttaatttttt atagtcaaat
     2521 acatctaact ttgaattttt taaatcccaa tgccagtaat attctgtagt tataacgata
     2581 taattctcga attctggtgt agaaaggcta agttgtatca gcttttctgc aagttgtaat
     2641 tgcctaaaat tgatgctgac ttcaattgcc cccaattcga ttaaatatgg aagcttacct
     2701 gtagaccagc gctcaatttc atcaggatag tggaaagtga cataacccac aatgagttga
     2761 tcttggcgag caacataaat tctaccttct ggtaatgttg taatttctaa caaagcttta
     2821 tactgatctt cagcatctct aaatgcggtt aaatgcgcat cgaaagtaag cgctttcaaa
     2881 tcttcgtgtg ttaaaggacc ttcaataaca aattgcttgt catgaatgta ataatcttcg
     2941 gattgatacg tctttaaatg attcatattt tcaactcctc aatcgacgtc gtgattgtat
     3001 taaattcatt atatagaaaa tttacaataa ttaatactag aaaaaggata aaagtaaaaa
     3061 ttttgaataa ttagaaatgt tatgtataat attgagaaag aaagcgtttt cacataacaa
     3121 agggggagtt tcaaatgaaa gtcgaagttt ataaaggagc gcaaggtaaa cataacctta
     3181 aagattatga agaaacatat aatacttttg attggaaaga cgtagaacaa gcattttctt
     3241 ggagtgaaac tggaaaaatg aacatggcat atgaatgcat agatcgccat gtagatcaag
     3301 gattagggga taaaatagcg ttaaattaca aagatgagca cagaaaagaa tcgtatactt
     3361 ataaagatat gcaacggtta tctaataaag cagcgaatgt tttgtctgaa catgcagaag
     3421 ttgacaaagg tgacagagta tttatattta tgtcgcgtac acctgaacta tattttgcgt
     3481 tgttaggtgt tttaaaaatt ggtgcaattg ttgggccgtt atttgaagca tttatggaaa
     3541 aggcagttgc ggatagatta gagaacagtg aagctaaagt gttaattact aataaggcat
     3601 tgttacctcg agtacctgta gataaattac caaacttgaa aaaaattgtt gtcgtagatg
     3661 aggatgtaga agacaattac atagacttca ttagtttgat ggaaactgct agcgatgaat
     3721 ttgacattga atggttaaag tcggatgatg gtttgatttt acattataca tcaggttcta
     3781 ctgggcaacc taaaggtgta ttgcatgttc aacaagcaat gttagtgcac tatatttctg
     3841 gaaaatatgt attagattta caagaagatg atgtttattg gtgtacagca gatccaggtt
     3901 gggttacagg aacatcttat ggtatttttg caccatggtt aaatggcgct acaaattgta
     3961 tagctggtgg tcgcttttcg ccagaacagt ggtatagtat gattgaagat tttaaagtga
     4021 cgatttggta tacggcacca acagctttaa gaatgttaat gagtgctggt gacgatattg
     4081 ttgagaaata tgacttgtca tcgttacgtt cgattctatc agtaggtgag cctttaaatc
     4141 ctgaagttat aaaatgggcg aaaaaagtat acggtttaac ggtgttagat acttggtgga
     4201 tgacagaaac aggtggacat atgattgtta actatccaac gatggacgtc aagcttggct
     4261 caatgggcaa accattacct ggtattcaag ctgcaattat cgatgatgca gggaatgaat
     4321 taccaccaaa tcgaatgggc aaccttgcta taaaaaaagg ctggccatca atgatgtatc
     4381 gtatctggaa gaatccagaa aaatataaat catattttat tggagactgg tatgtatctg
     4441 gtgattcggc atataaagat gaagatggtt acttctggtt ccaaggacgt gttgatgatg
     4501 taattatgac agctggtgaa cgagttggac catttgaggt tgagtctaaa ttggttgaac
     4561 acgaagcagt tgccgaagca ggaattattg gtaaacctga tccggttcgc ggtgaaataa
     4621 ttaaggcgtt tgttgcactg agaaaaggat atgaaccaac agacgaatta aaagaagaaa
     4681 ttcgtatatt tgttaaagaa ggtttgtcgg cacatgcagc accacgtgaa atcgaattta
     4741 aagataaatt acctaaaaca cggtcaggta aaattatgag acgtgtatta aaagcttggg
     4801 aattaaattt agatgctggg gatttaagta caatggaata atgacatgaa tgttattgaa
     4861 gatttttttc gaagaataaa gggtgacaac atatttcatg tcaatgttta aataatcgtt
     4921 tactttacga taagcaatat aaagaactgt taacttgtgt catatcattt cgtagaaagc
     4981 atttgaaaat gatgacataa caataatggc atatctttat attgcttttt atttttaata
     5041 tgatctttgg aagatgatta ttttaaataa tagaaaaata tagttatcaa tagtatcaag
     5101 cgctaaaagt tgtataatac aaaactttaa taagtgaatt tattgcaaaa atgaaagcgc
     5161 taacccgatt tagtcgacaa gtttttaaca gttcgttatt atatgaatgt aagtaaaaat
     5221 ttcttagcta caacttacat attataaatg cataaattaa acaaaaaggg gcgaaaaaag
     5281 ttgactcatt tatcagattt agatattgcg aatcaatcaa cactacaacc aattaaggat
     5341 attgctgcat cagtaggtat ttcagaggat gcattagaac cttatggtca ttacaaagct
     5401 aaaatcgaca ttaataaaat tacgccaaga gaaaacaaag ggaaagttgt tttagtaact
     5461 gcgatgagcc caacaccagc tggtgaaggt aaatcaacgg ttacagttgg tttagctgat
     5521 gcattccatg agttaaataa aaacgttatg gttgcattaa gagagcctgc tttaggacca
     5581 acatttggta tcaaaggtgg tgcgactggt ggtggttatg cgcaagtctt acctatggaa
     5641 gatatcaact tacatttcaa cggagatttc catgcgatta caactgcaaa taatgcattg
     5701 tctgcgttta tcgataatca tattcaccaa ggtaacgaat taggaatcga tcaaagacgt
     5761 attgagtgga aacgtgtatt agatatgaat gatcgtgcac ttagacatgt aaacgttggg
     5821 ttaggtggac ctacaaatgg tgtaccacgt gaagatggct ttaatattac agtagcgtct
     5881 gaaattatgg cgattttatg tttaagtaga agtattaaag acttaaaaga taaaattagt
     5941 cgtattacta ttggttacac tagagatcgc aagccagtta cagttgcaga tttaaaagtg
     6001 gaaggtgcac ttgcaatgat tttaaaagat gcaataaaac caaacttagt acaatcaatt
     6061 gaagggacac ctgcattagt tcatggtgga ccatttgcga atatcgcaca cggttgtaac
     6121 tcaattttag caactgaaac agcacgtgat ttagctgata tcgttgtaac ggaagctgga
     6181 tttggttcag acttaggcgc tgaaaaattc atggacatta aagtgcgtga agcaggattt
     6241 gatccggcag ctgtcgttgt tgttgcgaca attcgtgcgt taaaaatgca tggtggtgta
     6301 gcgaaagata atttaaaaga agaaaatgta gaagcagtaa aagcaggaat tgttaattta
     6361 gagcgtcatg ttaataatat taaaaaattc ggtgtagaac cggttgttgc aattaatgca
     6421 tttatacatg ataccgatgc agaagtagaa tatgtaaaat cttgggctaa agaaaataac
     6481 gtacgaattg ccttaactga agtttggaaa aaaggtggta aaggtggcgt tgacttagca
     6541 aatgaagtat tagaagtcat tgatcaacct aattcattta aacctttata tgaattagaa
     6601 ttaccattag agcaaaagat tgaaaagatt gtgactgaaa tctatggcgg ttcaaaagta
     6661 acgtttagca gtaaagcgca aaaacaatta aaacaattta aagaaaatgg ttgggataat
     6721 tacccagtat gtatggcgaa aacacaatat tcattctcag atgatcaaac gttgttaggt
     6781 gcaccatcag gatttgaaat tacaattcgt gaattagaag cgaaaacagg tgcaggattt
     6841 atcgtagcgt tgacaggtgc aatcatgact atgcctggtt tacctaaaaa accagcagca
     6901 ttaaacatgg atgttactga tgatggtcat gcaattgggt tattctaata aatcatgtca
     6961 attgtttaat aaagataagt aaatagttta atagaccgga ctgttggaga tgcattattt
     7021 cagcagttcg gttttttgct gtgctaaaaa tagattcaat ttggcgaatc taacgacaat
     7081 gtttgaaggt ggttaattaa tgtatatgaa gataaaaagt gggcttgaag aataggaaag
     7141 cgatgcaatg aatattccat attaaaaaaa aataataaaa taggttgcaa tatttaattg
     7201 ggatgcgcta caattaacac taataatagg agttgttaac aacatgaaca aacatcaccc
     7261 aaaattaagg tctttctatt ctattagaaa atcaactcta ggcgttgcat cggtcattgt
     7321 cagtacacta tttttaatta cttctcaaca tcaagcacaa gcagcagaaa atacaaatac
     7381 ttcagataaa atctcggaaa atcaaaataa taatgcaact acaactcagc cacctaagga
     7441 tacaaatcaa acacaacctg ctacgcaacc agcaaacact gcgaaaaact atcctgcagc
     7501 ggatgaatca cttaaagatg caattaaaga tcctgcatta gaaaataaag aacatgatat
     7561 aggtccaaga gaacaagtca atttccagtt attagataaa aacaatgaaa cgcagtacta
     7621 tcactttttc agcatcaaag atccagcaga tgtgtattac actaaaaaga aagcagaagt
     7681 tgaattagac atcaatactg cttcaacatg gaagaagttt gaagtctatg aaaacaatca
     7741 aaaattgcca gtgagacttg tatcatatag tcctgtacca gaagaccatg cctatattcg
     7801 tttcccagtt tcagatggca cacaagaatt gaaaattgtt tcttcgactc aaattgatga
     7861 tggagaagaa acaaattatg attatactaa attagtattt gctaaaccta tttataacga
     7921 tccttcactt gtaaaatcag atacaaatga tgcagtagta acgaatgatc aatcaagttc
     7981 agtcgcaagt aatcaaacaa acacgaatac atctaatcaa aatacatcaa cgatcaacaa
     8041 tgctaataat caaccgcagg caacgaccaa tatgagtcaa cctgcacaac caaaatcgtc
     8101 aacgaatgca gatcaagcgt caagccaacc agctcatgaa acaaattcta atggtaatac
     8161 taacgataaa acgaatgagt caagtaatca gtcggatgtt aatcaacagt atccaccagc
     8221 agatgaatca ctacaagatg caattaaaaa cccggctatc atcgataaag aacatacagc
     8281 tgataattgg cgaccaattg attttcaaat gaaaaatgat aaaggtgaaa gacagttcta
     8341 tcattatgct agtactgttg aaccagcaac tgtcattttt acaaaaacag gaccaataat
     8401 tgaattaggt ttaaagacag cttcaacatg gaagaaattt gaagtttatg aaggtgacaa
     8461 aaagttacca gtcgaattag tatcatatga ttctgataaa gattatgcct atattcgttt
     8521 cccagtatct aatggtacga gagaagttaa aattgtgtca tctattgaat atggtgagaa
     8581 catccatgaa gactatgatt atacgctaat ggtctttgca cagcctatta ctaataaccc
     8641 agacgactat gtggatgaag aaacatacaa tttacaaaaa ttattagctc cgtatcacaa
     8701 agctaaaacg ttagaaagac aagtttatga attagaaaaa ttacaagaga aattgccaga
     8761 aaaatataag gcggaatata aaaagaaatt agatcaaact agagtagagt tagctgatca
     8821 agttaaatca gcagtgacgg aatttgaaaa tgttacacct acaaatgatc aattaacaga
     8881 tttacaagaa gcgcattttg ttgtttttga aagtgaagaa aatagtgagt cagttatgga
     8941 cggctttgtt gaacatccat tctatacagc aactttaaat ggtcaaaaat atgtagtgat
     9001 gaaaacaaag gatgacagtt actggaaaga tttaattgta gaaggtaaac gtgtcactac
     9061 tgtttctaaa gatcctaaaa ataattctag aacgctgatt ttcccatata tacctgacaa
     9121 agcagtttac aatgcgattg ttaaagtcgt tgtggcaaac attggttatg aaggtcaata
     9181 tcatgtcaga attataaatc aggatatcaa tacaaaagat gatgatacat cacaaaataa
     9241 cacgagtgaa ccgctaaatg tacaaacagg acaagaaggt aaggttgctg atacagatgt
     9301 agctgaaaat agcagcactg caacaaatcc taaagatgcg tctgataaag cagatgtgat
     9361 agaaccagag tctgacgtgg ttaaagatgc tgataataat attgataaag atgtgcaaca
     9421 tgatgttgat catttatccg atatgtcgga taataatcac ttcgataaat atgatttaaa
     9481 agaaatggat actcaaattg ccaaagatac tgatagaaat gtggataaag atgccgataa
     9541 tagcgttggt atgtcatcta atgtcgatac tgataaagac tctaataaaa ataaagacaa
     9601 agtcatacag ctgaatcata ttgccgataa aaataatcat actggaaaag cagcaaagct
     9661 tgacgtagtg aaacaaaatt ataataatac agacaaagtt actgacaaaa aaacaactga
     9721 acatctgccg agtgatattc ataaaactgt agataaaaca gtgaaaacaa aagaaaaagc
     9781 cggcacacca tcgaaagaaa acaaacttag tcaatctaaa atgctaccaa aaactggaga
     9841 aacaacttca agccaatcat ggtggggctt atatgcgtta ttaggtatgt tagctttatt
     9901 cattcctaaa ttcagaaaag aatctaaata attaactaaa tatagcatat gtatgattaa
     9961 ctttgtagac aatgtgaaag caattaattt ataaactatt gattggttta atggctttcc
    10021 tttagagtaa ataaaaagaa cagcagtgag aaattttcta attgaaaata atcttactgc
    10081 tgtttttaat atttggattc attgttgtgg ttactttaaa aagtgagcat caattaacgc
    10141 ttttttcgat ttaacaaatg tgatttaata tcatatttta atgcgtcgtt gtattctttt
    10201 tcagtgattt gatcttcgat taacatacgc tttaatacat aatgttgtct ttgaatacta
    10261 tatttcaaat ctttatccga ttttaacgtt ccatcttttt cgtagggtgt atagccatat
    10321 gggctttgca acaaaccgat aaggtatgca gattgtgcaa ttgataaatc ttttggtgga
    10381 ataccaaata gactatatga agcggatgca attccggaaa tattagcgcc attataatct
    10441 ctaccgaagg gaactatatt taaatatgta tatataattt catcttttga gagtaggtgt
    10501 tctaatctaa ttgctaggcg aagttcattt gcttttctac tatatgtttt ttcgttggta
    10561 agaacttgat ttttaacaag ttgttgtgta attgtgctac cacctgaact ttgatcagta
    10621 ttaaaaatat cttgtatcat tgctcttaaa atcgcctttg gtaagatgcc atcatgttta
    10681 taaaataaag tgtcttcaga tgacgttaat gctttaatga catttggact tgatgtttta
    10741 gggcctataa tgagtgagtt ttgagaatgg ttatactcat ataataaatt tttgttatta
    10801 tgatctaata attcatcgcc aggtatttgt cgaacttttt ttattaaagc atcatctgat
    10861 aatgagtcgg acgttttagt taaatgatga aaatataaag acatcgcaat cacagcgata
    10921 gcaacgatgg caatgaaaat cataaatata aaaacaatga catgtttgat agctttatat
    10981 atcttttcaa aatgatatat acgatgattc aattgatgat tgttgtcttg attcgtcatc
    11041 tttaacctcc taaattacgt atagtatacc atatttaagc aatttgactt tta
//
LOCUS       DAKTIP010000078        11092 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAKTIP010000078 DAKTIP010000000
VERSION     DAKTIP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.078"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            24..719
                     /locus_tag="O0305_001488"
     CDS             24..719
                     /locus_tag="O0305_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0274636.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNKPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            721..1491
                     /locus_tag="O0305_001489"
     CDS             721..1491
                     /locus_tag="O0305_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274637.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1864..3828
                     /locus_tag="O0305_001490"
     CDS             1864..3828
                     /locus_tag="O0305_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCY0274638.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQNCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKDLQAQIDILKMLMHDRYLD"
     gene            4172..5251
                     /locus_tag="O0305_001491"
     CDS             4172..5251
                     /locus_tag="O0305_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274639.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMVVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5356..5949)
                     /locus_tag="O0305_001492"
     CDS             complement(5356..5949)
                     /locus_tag="O0305_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274640.1"
                     /translation="MEHIFREGQNGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYDFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFNTRGAQVEEVWVKGHEITETGLTAGQQILGK"
     gene            complement(5971..6939)
                     /locus_tag="O0305_001493"
     CDS             complement(5971..6939)
                     /locus_tag="O0305_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCY0274641.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHG"
     gene            complement(7017..7451)
                     /locus_tag="O0305_001494"
     CDS             complement(7017..7451)
                     /locus_tag="O0305_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0274642.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7678..7962
                     /locus_tag="O0305_001495"
     CDS             7678..7962
                     /locus_tag="O0305_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0274643.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLLKAVVEHARENNLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8133..8939
                     /locus_tag="O0305_001496"
     CDS             8133..8939
                     /locus_tag="O0305_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0274644.1"
                     /translation="MAFHDKTATQVTNIVLNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNSQSYGLTHVDIYQPDASTHTFTAPEGFDITVHSNTDLVPEK"
     gene            complement(9059..9730)
                     /locus_tag="O0305_001497"
     CDS             complement(9059..9730)
                     /locus_tag="O0305_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCY0274645.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQEPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10000..10992
                     /locus_tag="O0305_001498"
     CDS             10000..10992
                     /locus_tag="O0305_001498"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0274646.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
BASE COUNT         3622 a         1950 c         1766 g         3754 t
ORIGIN      
        1 ttaatagcaa aggagatagt gatatggatg ttttaacaat agaacattta acaaagaaga
       61 taggcaacaa aacgattctc gaagatgtat catttaagct gaaacgcgga caaatagttg
      121 gtctcgttgg agcgaatggt gcaggtaaaa caactttaat gaaagttata ttaggttact
      181 ctagtttcca aagcgggaat tttaatgtta ttaacagcaa ggacagcaaa agcaatatcg
      241 gcgcattgat tgaaaatcca ggaatatatc cttttatgtc tggatatgaa aacttgaagt
      301 tattgaatga atcaaaaaac actcaagata tcgataaaat tgtctcacaa cttcatatgg
      361 atgaatacat tcataaaaaa gctaaaacgt attctcttgg tatgaaacaa aaattaggaa
      421 ttgctatagc atttttaaat aaacctcaat tcattatctt agatgaacca atgaatggct
      481 tagatccaaa agctgtgcga gatgtacgtg aattgattgt ccaaaaagcg caagaaggtg
      541 ttactttctt aatttcgagt catattttaa gtgaattagt taaaatcaca aactctatcc
      601 ttattattaa caaaggtaaa attgttacag aaacatcgga agaagaactt aaacaattta
      661 aagataatga tttagaaaat gtattactag aaatcataga aagggaggac caagcataaa
      721 atgggaactt taattaaaca agaatgtttc aaattattta aaaagaaatc aacttttatc
      781 gcacctattg tctttattct actaatggtt gctcaaggtt atattgctac aaaatacaat
      841 gaaattttta cgccacagga atctttcaca tctgcttata atggtttttc atggtttgca
      901 tttttattaa ttattcaagc aagtacaatc atttcaatgg aatttcatta cggtacgatt
      961 aaaaatttac tctatcgtga atattcaaga acaactatga ttgttagcaa aatcatcaca
     1021 ttatttatta tttctttaat ttattttgtt attacaatta ttgcttcaat tgttattggg
     1081 tctttattct ttaatgattt aaatatattt gaaagtagcg gtaatcaatt atctttattg
     1141 aatcaattat tattagttag tttaggcaca tttgttggcg tttggttagt tttaagctta
     1201 acgttgctat tatcatctgc aacaaattca acgggagtag ccattgctgt aggtattgtt
     1261 ttttattttg caagttctat tttagcagtt attcaaacgg cacttttaga aaaaatagac
     1321 tggctaaagt ggaatcctat taatatgatg aatattatgc ttcaaacagt tgaaaaaggc
     1381 tttagtaagt cgacaaaatt agaacttcat gaattgttta ttggtaatat tgcttatatt
     1441 tctattttct taatacttgt agtatttatt ttcaagaaga aaaatattta gtaacttaaa
     1501 gtattaaatg tctaaataca cacatattcc atcgtaattc aaaatcattt tcaaatccct
     1561 tcacccaaat aatggtgcgg ggattttttc atccaaattt tggaaattcc acattttaca
     1621 tatcgtaatt ttttaggaaa ctagtgaata taacaaatcc ctcctctcat ttttaaaata
     1681 gatatatcac ttccccactt ttacttaact aaactgcaac ggttcctaat accaaaatcc
     1741 tgccctctat ttttatcaat tcaagcatac ttattgaaaa atgttaacgt tttcttgata
     1801 atcattgtaa gcgcatttat tttataaact aacgtttgaa atatactaca ggagtgacac
     1861 gtaatgactc aaattactga aaaagaatta aaaaagaagt atttagattt actatcccaa
     1921 aattttgata ctccagaaaa acttgcaact gaaattatca atttagaatc aattttagaa
     1981 ttacctaaag gtacggaaca tttcgtcagc gatttacatg gtgaatatga agctttccaa
     2041 cacgtattac gcaacggttc tgggaacgtg cgagcgaaaa tcaatgatat tttcaaagag
     2101 agactttcaa ctaaggagct taatgactta actgctcttg tctactatcc agaagacaaa
     2161 ttaaaattga ttaaaagtga tttccaaaat tgcggtcaac ttaatgtctg gtatatcaca
     2221 acaatcgaac atttaattga gttaattaaa tattgttctt caaaatatac gcgttcaaaa
     2281 ctgcgtaaag cattgccaaa acaatacgtt tatattattg aggaactact gtataaaagt
     2341 aatgaatatc aaaataaaaa atcatattac gaaacacttg ttaaccaagt aattgaactt
     2401 aaacaggcag atgatttaat tattggactc gcttattccg tacaacgctt agtcgtcgat
     2461 catttacacg ttgtcggtga tatttatgat cgtggaccac aaccagataa aattatggat
     2521 acactgatta attatcattc cctagatatt caatggggta atcatgatgt gctttgggtt
     2581 ggagcctatg ctgggtcaaa agtatgctta gcaaacttac ttcgaatttg tgcacgttat
     2641 gataatttag atattatcga agacgcttat ggcattaatt taagaccact gcttacttta
     2701 gctgaaaaat actatgacgc agataatcct gcttttaagc ctaaaaaaag acctgacaaa
     2761 cacgaacgtt taactcaacg tgaagaaagt caaattacta aaattcatca agctattgcg
     2821 atgattcaat tcaagttaga aataccaatt attaaacgtc gtccaaattt cgaaatggaa
     2881 gaacgtcttg tgcttgaaaa ggttaattat gatacaaatg aaattacagt ttatggtaat
     2941 acatacccat tgaaagacac atgtttccaa actgtcaatc gtgataatcc agcagaatta
     3001 ctacctgaag aagaagaagt catgaataaa ctattattgt cattccaaca atctgaaaaa
     3061 ttacgtcgtc atatgtcttt cttgatgcgt aaaggctctc tttacttacc atataatggc
     3121 aatttactca ttcatggttg tattccagtt gatgaaaatg gtgagatgga atcatttgaa
     3181 attgatggtc atacttacag cggccaagaa ttattagatg tgtttgagta tcatgtccgt
     3241 aaatcatttg atgaaaaaga aaatactgat gacttatcga cggatttagt ttggtattta
     3301 tggactggga aatattcgtc actatttggt aaacgtgcca tgactacgtt tgagcgatac
     3361 tttattgcag ataaagcttc tcataaagaa gaaaagaatc cgtactatca tcttcgtgaa
     3421 gatgtgaata tggttcgtaa aatgctcagt gatttcggat taaatccaga tgaaggacgc
     3481 attattaatg gtcacacacc agtgaaagaa atcaatggcg aagatcctat caaggctgat
     3541 ggaaagatgc ttgtcattga tggtggcttt tcaaaagctt atcagtcaac aactggcatt
     3601 gcaggctata cactattgta taattcattc ggtatgcaac tcgttgcaca ccaacaattt
     3661 aacgctaaag aaaaaatact ttccgaaggt atcgatgaac tttctataaa gcgcgtcgta
     3721 gataaagaat tacaacgtaa gaagattcgt gataccaata ttggtaaaga tcttcaagct
     3781 caaatagata ttttgaaaat gttaatgcat gatcgttact tagactaaag acgacatttg
     3841 atgcatgtat cgtgcactta tttagtggta ctgtgtttac gcattggcta cgcaaacacg
     3901 atgataaata ggcgacctag ctacaccgaa aaaaatcccc tcactattgc gagtagtgag
     3961 gggattggtg tatctatgct aattcctgtt aactgtatta taacaacttt acagcaaaaa
     4021 aatgcaaaat caactaataa attaatgaaa tgaccgacac cctcgcttct actccaattt
     4081 aacaaaatac tatattagta ctatattttt cattaacatg tatgttacat tttctatatt
     4141 acttaaaatt acgaagggac atttttaaat catgcgtatc ttaaaagagt ccattattgt
     4201 ggcatttgcc tttgttggtg ttgtcgttgg tgccggcttt gctactggtc aagaaatttt
     4261 ccagtttttc acaagtcatg gcgcatatag catttcaggc attattgtaa caggactatt
     4321 gattacttta ggtggaatgg ttgtcatgca tacaggtcat catctaaagt ccagaaatca
     4381 ttctgattca attaactatt tcttataccc ctctattgca agaggttttg atattatttt
     4441 aacaatgttt atgttgtctt tagctattat tatgactgca ggtggtgcgt caaccattca
     4501 tcaaagtttc aacttaccgt attggctgag cgcactcata ttagtcgcct ttattttagc
     4561 aacactgttt ctaaaattcg atcgtttaat tgctgtgctt ggcggtgtta ccccattttt
     4621 aattgcgatt gtcattatga ttgcggtcta ctatttcaca acaagtcatc ttgattttac
     4681 tgccgctaat aatgatgctc agattcataa gcagaaatca ttatcacctg gatggtggtt
     4741 tgatgcgatt aactatgcaa gcttgcaaat tgctgctgcc ttcagcttct tatcagtgat
     4801 gggtagtaaa gttaaatatc gtgactcaac gttatacggg ggcttgattg gcggtttaat
     4861 cattacattt ttactcatga tgattaatct aggtttaatt tctcaattcg ataaaattaa
     4921 acacgtagat ctacctacat taaaattagc gacacaaatg tctccgtcaa ttggtattat
     4981 tatgtctgtc attatgatac ttgtcatcta caatactgtt gttggattaa tgtatgcatt
     5041 tgcgtcacgt ttcagcgttc cattcagcag acgttacttc atcattatta ttacaatggc
     5101 tgtcatcact tatattagta catttatcgg tttcatttca ttaattggaa aagtattccc
     5161 tattatggga ttgttcggtt tcatcttact catacctgta ctctataaag gtttaattaa
     5221 gcgtattacc ggcaaatctc atatcgatta aaatatctat actattgtaa aatttagtac
     5281 agcacttcaa aatcaaaaaa gcgggcagat gaaaagtaaa aatcttttcc atctgtccgc
     5341 ttcttaatag aactgttatt tcccaagtat ttgttgacca gccgttaatc cagtttctgt
     5401 aatttcatgg cccttcaccc aaacttcttc gacttgtgcc ccacgtgtat taaacaagtt
     5461 aatgacttgt tcacttgcag ctaatggcac aattggatca tgtttcccca tagaaagcaa
     5521 cacactgaca tctgataaat cctttgttga cgttacttca actgggtata acggtgcata
     5581 taacaatgct tttttaaatg gtgcttctga acgcaacatt aagttaatcg ctatatttga
     5641 tccatttgaa aatccaacaa gtactgcttt ttcaatatca aaatcataac gttcagcagc
     5701 ttctttaatg aacgtcaaca attcttgtcc acgaaatgcc aaatcttctt catcataaac
     5761 accttcacca agacgtttga aataacggtt catcccattt tctgaaactt gtcctctaat
     5821 acttaacaag tgataatttt cattcaatgc ttcgcctaac ggtaataaat cgaactcatc
     5881 accacctgta ccatgcaata atattagtgt tggcgcacca ttttgtcctt ctctaaaaat
     5941 atgttccatc acaaatcctc ctcattccaa ttaaccatgt tgacgcttcg tattaaaagg
     6001 tctaacttcc gattcaatat attctctttt attttctaaa aatggtggta aggataaccc
     6061 ttcgcctaat gtttcataag gttcatcttc cataaatcct ggtccatctg ttgaaatttc
     6121 tattaaaata tgccccacac gtgcatataa tgcttcaaaa tagaaacgat taacgatgcc
     6181 tgagttatta atacctacct ctttatattt cgttgcccac gcttctattg catcatgatc
     6241 tttcacacga aatgacacat gatgtacctc accataacct tgacgtgctg ctggcccttt
     6301 atcatctttt attaagatta cctggccacc attgcctcct tcgccaactt caagtaatgc
     6361 gacattatct tcatgcgcaa tagttgtcat accgtaaaca gtctctaaaa tatttttaaa
     6421 gtcgtcaaaa taacttactt taatttcaat ggggcctaat ccataaatcg ctttatctac
     6481 tggaaccggt ccattcttcc aaggtacacc aggtgctacc ccttcattta actcatctga
     6541 aattaattga tacacttggc catcgacttc ttcaaatggc aacacttttt taccaaataa
     6601 ttcttgaata ccttcgtgtt taacaccaaa ctcatcaaag cgctgttcat aatatgttaa
     6661 tgcgtcatcg ttaggcactc taaaagacgg tcttgtaatg gaatttgttc ctgcctgccc
     6721 ttttgtaata tttggaaaat caaagaacgt catgtctgta cctgccgaac ctacatcatc
     6781 tgcaaaaaaa gtatgatacg tataaatatc atcttgattg actgtctttt taactaaacg
     6841 catgcctagt acttctgtaa aaaatttata attacgttct gcatcatctg tcattgcagt
     6901 aacatggtga atacctagta attcatgatt atttatcatt ttatataccc tctttatatt
     6961 tttagtgcat ttatctcgaa atcgagatac tttaatgtaa atttttagtg cacgccttac
     7021 acttctgtag attgtgcact tagtttctta aacgcttgtt gtaagattgt taattcatcc
     7081 tttgttaaca catcaaacgc ttttgttaat gtctcagcat gcttagggaa aatatctgcc
     7141 atttgacttt gacctttttc agttaaacaa gccatatata cacgtttatc atctttatcc
     7201 ttttcacgtg taatccaacc tttgtcctct aattgactta caacatatga aatgctgcta
     7261 cttgcaatta atacgcggtc tctaatacgt tgaattggtt gcggaccttt attataaagc
     7321 aactcgagca ctgcaaattc agtaatattt aagccatatc gctttacgtc ttcttttgta
     7381 atttgctcta atgtgtctaa cgccctattc attccgacaa aaacattgag agattgtttc
     7441 gttcgatcca tatgatcacc aactttttta ttttatttcg atttcgagat aaattatata
     7501 gctgaataga tttaatgtca agatacgtgt tttcatcttc atttcaaact gtgcttaata
     7561 aaaacgacta catagatgtg gcaatgtcgt cacatgactg tcatgcacct ttgacttcgt
     7621 ttaagttaaa atagaatagg gaaatactat aatcaatcat gataggagtt gatatgtatg
     7681 agtaaccttg aaatcaaaca aggcgagaac aaattctata ttggtgatga tgaaaataat
     7741 gctttagctg aaatcacata ccgttttgtg gataataatg aaattaacat tgatcataca
     7801 ggcgtatctg atgaacttgg tggtcaaggt gttggcaaaa aactacttaa agcagttgtt
     7861 gaacacgctc gagaaaataa tttgaaaatt attgcctcat gttcatttgc caaacatatg
     7921 ttagaaaaag aagattcata tcaagatgta tatcttggtt aaataaattt tgcgatggtg
     7981 caactgtacc atcgcttttt tgttcgcatc aatataaaat tgcaaaacga ctagttattt
     8041 tgcatttcaa actttatgct atcaattcat tttcttttct ggtatacttt ttatactaac
     8101 ttatagaaaa atagaaaaag aggcgatgca taatggcatt tcacgataaa acagcaacac
     8161 aagtgacaaa cattgtactg aacgtaagag acttagactt aatgacaaca ttttacaaaa
     8221 acattttagg actatccgtt aaaagttctg acgataatac aaccgtatta tctgttggga
     8281 ctggtggcca tactctaacg ttacatttat tagaagacgg ccgtcagact tccccacgtg
     8341 aagcagggct ttttcatata gcatttttat taccaactac tgaggatcta gctaacttct
     8401 tatatttcgt ggcacaaaaa aatatgggca tcggcgctgg tgatcattta gtaagtgaag
     8461 ctttatattt caacgacccc gaaggtaatg gtattgaagt ctatcgcgat agaccttcat
     8521 cttcatggga atggcaaaat ggcaaagtta aaatggatac tttagaagtt gatagccaaa
     8581 ccttattaac acatcgtact gatgaaggtt ggcaaggaat gccagcaaaa ggcatgattg
     8641 gacacttaca tttaaaaaca catgatttag acgcagcata tcaatgttac attgaacaat
     8701 taggattcca acatgtgtct gattttccac gtgcgctatt tatgtcaacg aatcattatc
     8761 accatcatat tgctgctaat acttggcaat ccaatcaagc aagacaaaac aattcacaaa
     8821 gttatggatt aacacatgtt gatatttatc aacccgacgc ctcaacacat acatttacgg
     8881 caccagaagg gtttgacatc acagttcatt caaatactga ccttgttcca gaaaaataat
     8941 catccatact ttattaatac aattttttca cgcaaaagcc tgcgattcat ttatgtaatc
     9001 gcaggctttt ttattgctaa ctttttaatt ttatatattt agacatacgg tattctattt
     9061 attcttttgg tccaacccat tcaataacat cttcataagc ttggcgtgtt ttatttttcg
     9121 gtggctcttg ttgtctgtag ccaaatgcga ccatcactga taaaccaaat tgctcagtat
     9181 ctaagatccc tttatttgct aaaatgtctg tcactgtatc cagactaaaa ccttccatcg
     9241 gacatgaatc aatacctaac aatgcggctg tcgtcatcat attgcctaat gcaatatacg
     9301 tttgtttact tgaccagtca tacaaggctt gatcattatc agaaatatgg aaatctgctt
     9361 ggaatgcatc gaatttttgt tcaacagctg gaatcgtttg tgcctcatat tttttaatat
     9421 ctcttaacat atgttgtaca tacggtgatc ttgacgttac atttttacgc gcaaaaatta
     9481 gcacaaaatg gctcgctgta tctaattgtt ttgctgcgcc ccaactgtgc gcttttaatt
     9541 catcacgtaa cgcttgatct tgaatcacga caaacttcca aggttctaac ccaagagaac
     9601 ttggagacaa tctacctgac tctaatattg tttcaaaatc ttctttcgaa actttctttt
     9661 gtggatcgaa ttgcttagtc gcatgtctga aatgaaatgc atccataatt gtttgattca
     9721 tattgctcat tgaacatagc ctcccatttt tatatttatt gctatacaaa ttatacttca
     9781 tgttttaaaa agactaaagt aatccgtttg caattcacta taacttgtgc acatgttata
     9841 agttttctgc aataaaacac acttttgaat ttcgggaaat ttagtgttat aatatatttg
     9901 tgatattttt cacaattaaa ttgtggcgtg attttaatgt gcataatctt taatttcact
     9961 catttacttt tcaatctttt aaaagcgagg tttttaataa tgacaaaaat tatgttcttt
    10021 ggtacgcgtg attatgagaa agagatggca ttaaattggg ggaaaaagaa taatgtcgaa
    10081 gtaactactt ctaaagagct attatcaagt gctacagtcg atcaattaaa agattacgat
    10141 ggcgtaacta caatgcaatt tggtaagtta gaaaatgacg tttatcctaa attagaatct
    10201 tacggtatta aacaaattgc acaacgtact gctggatttg atatgtatga tttagattta
    10261 gctaaaaaac acaatattgt gatatctaac gttcctagtt attcacctga aacaattgca
    10321 gagtattctg tttctatcgc cctacaatta gtgcgtcgct tcccagatat tgaacgccgt
    10381 gtacaagcac atgattttac ttggcaagca gaaatcatgt ctaaaccagt taaaaatatg
    10441 actgttgcaa ttattggtac gggtcgtatc ggtgctgcta cagctaaaat atatgcagga
    10501 tttggtgcta caattacagc ttatgacgcc tatcctaata aagatttaga ctttttaact
    10561 tataaagata gtgttaaaga agctattaaa gatgccgata ttatttcttt acatgttcca
    10621 gcgaacaaag aaagctatca tttattcgat aaagcaatgt ttgatcatgt gaaaaaaggt
    10681 gcaatcttag ttaacgcagc acgtggtgca gtcatcaata cacctgattt aatcgctgca
    10741 gtgaacgatg gtactttgtt aggtgctgcg attgatactt atgaaaatga agcagcatac
    10801 ttcacaaatg actggactaa taaagacatt gacgataaaa cattattaga gttaatcgaa
    10861 catgaaagaa ttttagtaac accacatatt gctttcttct ctgatgaagc agtacaaaac
    10921 cttgttgaag gtggtttaaa cgcagcatta tctgtcatta acactggtac atgtgaaaca
    10981 cgtttaaatt aatatatata tttaaaagca aaggcgcgcc atgtgccttt gctttttatt
    11041 ttgtatatct gtatcaaaat cgatttgatt aaaatccgct ttattttatc at
//
LOCUS       DAKTIP010000079        10994 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAKTIP010000079 DAKTIP010000000
VERSION     DAKTIP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.079"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            265..2454
                     /locus_tag="O0305_001499"
     CDS             265..2454
                     /locus_tag="O0305_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCY0274647.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            2466..2918
                     /locus_tag="O0305_001500"
     CDS             2466..2918
                     /locus_tag="O0305_001500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCY0274648.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            2915..3790
                     /locus_tag="O0305_001501"
     CDS             2915..3790
                     /locus_tag="O0305_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCY0274649.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            4251..5513
                     /locus_tag="O0305_001502"
     CDS             4251..5513
                     /locus_tag="O0305_001502"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCY0274650.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            5529..7295
                     /gene="aspS"
                     /locus_tag="O0305_001503"
     CDS             5529..7295
                     /gene="aspS"
                     /locus_tag="O0305_001503"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCY0274651.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            7373..7563
                     /gene="ssrS"
                     /locus_tag="O0305_001504"
     ncRNA           7373..7563
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="O0305_001504"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7628..7756
                     /locus_tag="O0305_001505"
     CDS             7628..7756
                     /locus_tag="O0305_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274652.1"
                     /translation="MGFIAFNKCFLVEFWENNQSDNAKYKILSYYVLMYKSVKEVK"
     gene            7756..8529
                     /locus_tag="O0305_001506"
     CDS             7756..8529
                     /locus_tag="O0305_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCY0274653.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(8690..9964)
                     /locus_tag="O0305_001507"
     CDS             complement(8690..9964)
                     /locus_tag="O0305_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCY0274654.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            10049..10471
                     /locus_tag="O0305_001508"
     CDS             10049..10471
                     /locus_tag="O0305_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0274655.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            10571..10753
                     /locus_tag="O0305_001509"
     CDS             10571..10753
                     /locus_tag="O0305_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0274656.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKVIGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            10793..10939
                     /locus_tag="O0305_001510"
     CDS             10793..10939
                     /locus_tag="O0305_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274657.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
BASE COUNT         3955 a         1529 c         2159 g         3351 t
ORIGIN      
        1 tatttcataa attgtagtat catagaacta atatttaaaa aatgaaacag tagatttagg
       61 tcgaattttt gtaaaagttt taaaagtagg aatagtatac aaattaaact cgctcaagta
      121 aaattaatat tacgattaat gacgacagga taaatattta tcgtcgacgg acgtatgatt
      181 ggtgtgggac aaatactatt caacaagagt acctaaatca ttgtttaagg cgaagtaata
      241 aatatgaatg gggtgtatca tataatgaac aacgaatatc catatagtgc agacgaagtt
      301 cttcacaaag caaaatcata tttgtcagca gatgaatatg agtatgtttt aaaaagctat
      361 catattgctt atgaagcaca taaaggtcag ttccgaaaaa acggattacc atacattatg
      421 catcctatac aagttgcagg tattttaaca gaaatgcgat tagacggacc gacgattgtc
      481 gcaggttttt tgcatgatgt aattgaagat acaccgtata catttgaaga tgtaaaagaa
      541 atgttcaatg aagaagttgc tcgaattgtt gatggtgtga cgaagcttaa aaaagtaaaa
      601 taccgctcaa aagaagaaca acaagctgaa aatcatcgca agttatttat tgcgattgcc
      661 aaagatgtac gcgtaatttt ggtgaaatta gcagacagat tacataatat gcgtaccttg
      721 aaagccatgc cgcgcgaaaa acaaattaga atttctcgag aaacattaga aatttatgca
      781 ccattagcac atcgtcttgg tattaataca atcaaatggg aactagaaga tacggctctt
      841 cgttatattg ataatgtgca atattttaga atagtcaatt taatgaagaa gaaacgtagt
      901 gaacgtgaag cgtatatcga aacggctatt gatagaatac gtactgaaat ggaccgaatg
      961 aatatcgaag gcgatataaa tggtagacct aaacatattt acagtattta tcggaaaatg
     1021 atgaagcaga aaaaacaatt tgatcaaatt tttgatttgt tggcgatacg tgttattgtc
     1081 aattctatta atgattgtta tgcgatactt gggttggtgc atacgttatg gaaaccgatg
     1141 ccaggacgtt ttaaagatta tattgcaatg cctaaacaaa atttgtatca gtcattgcat
     1201 actacagtag taggcccaaa tggagacccg ctcgaaatcc aaatacgaac gtttgatatg
     1261 cacgaaattg ctgagcatgg tgttgcagca cactgggctt acaaagaagg taaaaaagta
     1321 agtgaaaaag atcaaactta tcaaaataag ttaaattggt taaaagaatt agctgaagcg
     1381 gatcatacat cgtctgacgc tcaagaattt atggaaacct taaaatatga cttacagagt
     1441 gacaaagtat acgcatttac cccagcgagt gatgttattg agttgccata tggtgctgtg
     1501 ccgattgatt ttgcttatgc gattcacagt gaagtaggta ataagatgat tggtgccaag
     1561 gtgaatggca aaattgtacc aattgactat attttacaaa caggcgatat tgttgaaata
     1621 cgtactagta aacattcata tggaccaagt cgtgattggt tgaaaattgt taaatcgtct
     1681 agtgccaaag gtaaaattaa aagtttcttc aaaaaacaag atcgttcatc taatattgaa
     1741 aaaggccgaa tgatggttga agctgaaata aaagagcaag gatttagagt cgaagatatt
     1801 ttgacagaga aaaatattca ggttgttaat gaaaaatata actttgcaaa tgaagatgat
     1861 ttattcgcag ctgtaggatt tggcggcgtg acatccttac agattgttaa taaattaact
     1921 gaaagacaac gtattttaga taaacaacgt gctttaaatg aagcacaaga agttacgaaa
     1981 tcattgccta ttaaagacaa catcattact gatagtggtg tctatgtaga aggtttagaa
     2041 aatgtactta tcaagttgtc aaaatgttgt aatcctatac caggtgatga tattgtaggt
     2101 tatatcacca aaggtcacgg tattaaagta catcgcactg attgcccaaa tattaagaac
     2161 gaaactgaac gactaattaa tgttgaatgg gtaaaatcaa aagacgcaac tcaaaaatat
     2221 caggttgatt tagaggtaac tgcgtatgac cgaaatggct tgttgaatga agtactacaa
     2281 gctgttagct cgacagccgg caatttaatt aaagtttcag gacgttcaga tattgataaa
     2341 aatgcaataa taaatattag tgtcatggtg aaaaacgtga atgatgttta tcgtgtggta
     2401 gaaaagatca aacaacttgg tgatgtttat acagtaacaa gagtttggaa ctagaggtgc
     2461 aaaatatgaa agtagttgta caaagagtta aagaagcatc ggtgacgaat gatacattaa
     2521 ataatcaaat caaaaaagga tattgtttat tagtcggtat cggtcagaac tctacagagc
     2581 aagatgcaga tgtaattgca aagaaaattg ctaatgcaag attatttgaa gatgacaata
     2641 ataaattaaa ctttaatatc caacaaatga atggtgaaat actatcagtt tcacaattta
     2701 ctctctatgc agatgtaaaa aaaggtaacc gtccaggttt ctcaaattct aaaaatcctg
     2761 atcaagcggt aaaaatttat gagtatttta atgatgcgct acgagcgtat ggtcttactg
     2821 tgaaaacagg tgaatttgga acacacatga atgttagcat aaataatgat ggtccagtca
     2881 ctattattta tgaaagtcag gacggcaaaa ttcaatgaaa aaaatagagg catggttatc
     2941 taaaaagggt cttaaaaata aacgtactct aatagtagtg attgcctttg tcttatttat
     3001 catcttttta tttttattgc tgaatagcaa tagtgaagat agtgggaaca tcacgataac
     3061 tgaaaatgct gaattacgta caggtccaaa cgctgcgtat ccagtcatat ataaagttga
     3121 aaaaggtgac cattttaaaa agattggtaa agtaggtaaa tggattgaag ttgaagatac
     3181 atccagtaat gaaaaaggtt ggatagctgg atggcacaca aatttagata ttgtcgcgga
     3241 taatacgaag gagaaaaatc ctttgcaagg taaaacaata gtgcttgatc ctggtcatgg
     3301 aggtagtgac cagggtgctt caagcaatac taaatataaa agtttagaaa aagattatac
     3361 gttgaaaaca gcaaaagaat tgcagcgtac tttagaaaaa gaaggcgcaa ctgttaagat
     3421 gacaagaaca gacgatacat atgtttcact agaaaatcgt gatatcaaag gcgatgccta
     3481 tttgagtata cataatgatg cgttagaatc atctaatgca aatggaatga cagtttattg
     3541 gtatcatgat aatcaaagag ctttagcaga tacgttagac gctacgattc agaagaaagg
     3601 tctactttct aatcgcggtt caagacaaga aaattatcaa gtgttaagac aaacaaaagt
     3661 tcctgctgtt ttattagaat taggttatat tagtaaccca actgatgaaa cgatgattaa
     3721 agatcaatta catagacaaa ttttagaaca agcaattgtt gatggcctta aaatttattt
     3781 ttctgcgtag ggcttgcaaa aatatgtgaa agtagttatc attgatattg aattttataa
     3841 ctaaaaccgt tagtattctt gaaatggtaa atgaaatagg tagcaatcta actaagattg
     3901 tgtaggaata taatccatag actgaaagat tatgctgagt agtttatata cattgaacac
     3961 aagaagaggt gctttatgaa aagtaaagcc gttaaacgta cgttaaacgt tttgagtggg
     4021 tttattaaat gcacgcttat aaaaagtaat gatgattaca attaggcatg ttttttaaac
     4081 caatgagggt ggcaacacgg atattcgtcc cttgtgcagt ttcaggctgt gcaagggctt
     4141 tttcttttgc ttttaagatt atgatttatc gtgcaaagtt aagtggtcgt atatagtttt
     4201 agttttaaaa aggtaattaa ataaaatagt ttgccgaggg agatgtcaaa atgattaaaa
     4261 tacctagagg gacgcaggat attttacctg aagattcaaa gaaatggcgt tacattgaaa
     4321 atcaattaga tgaattaatg acattttata attataaaga aataagaaca ccaatttttg
     4381 aaagtacaga tctttttgca agaggtgttg gtgattcaac cgatgtcgta caaaaagaaa
     4441 tgtatacatt taaagataaa ggcgatagaa gtattacatt aagacctgag ggaacagctg
     4501 cagttgtgcg ttcatatatt gaacataaaa tgcaaggtaa tccaaaccaa ccaattaaac
     4561 tttattacaa tggaccgatg tttagatatg aacgtaagca aaaaggacgc tatcgtcaat
     4621 ttaatcaatt tggtgtagaa gctattggtg ctgaaaatcc tagcgtagat gcagaagtat
     4681 tagctatggt tatgcatatt tatcaatcat ttggattaaa acatttaaag cttgttatta
     4741 atagtgtagg ggatatggcg tctcgaaaag aatataacga agcgttagtg aaacactttg
     4801 aaccagtaat tcatgaattt tgttcagatt gtcaatcacg tttgcataca aatccgatgc
     4861 gaattttgga ttgtaaagta gaccgtgata aagaagcgat taagactgca cctagaatca
     4921 ctgatttctt aaatgaggaa tctaaggcat attatgaaca agtaaaagct tatttagatg
     4981 atttaggtat tccatatatt gaagatccta acttagttcg tggattggat tattatacac
     5041 atacagcatt tgaattaatg atggataacc ctaactatga tggtgccatt acaacgcttt
     5101 gtggtggtgg ccgttataat ggtttattag aattgctaga tggtccaagt gaaacaggta
     5161 ttggttttgc gctaagtata gaacgattat tgcttgcact tgaagaagaa ggtatcgaat
     5221 tagatattga agaaaactta gatttattca ttgttacaat gggtgatcaa gcagatcgat
     5281 atgctgtgaa gctattaaat catttgagac ataatggtat taaagcagat aaagactatt
     5341 tacagcgtaa aattaaagga caaatgaaac aagcagaccg tttaggtgcc aagtttacaa
     5401 tcgttattgg tgatcaagaa ttagaaaata ataaaatcga tgttaaaaat atgacaactg
     5461 gtgaatctga aacaattgaa ttagacgcat tagtcgaata ttttaagaag tagagagggc
     5521 gttaaaatat gagtaagaga acaacttatt gtggattagt tactgaggca tttttaggac
     5581 aagaaattac attaaaagga tgggttaaca atcgtcgtga ccttggtgga ttgattttcg
     5641 ttgatttaag agatagagaa ggaattgtac aagtcgtgtt taatcctgca ttttcagaag
     5701 aggcattgaa aattgctgaa acagtacgtt ctgaatatgt tgtagaagtt caaggtacag
     5761 ttacgaagcg tgaccctgaa acagttaatc ctaaaattaa aactggccaa gttgaagtac
     5821 aagttacaaa tattaaagtg attaataaat ctgagacacc accattttct ataaatgaag
     5881 aaaatgttaa cgttgatgaa aatattcgat taaaataccg ttatttagat ttacgtcgtc
     5941 aagagttagc gcaaacattt aaaatgagac atcaaattac acgttctatt cgtcaatatt
     6001 tggatgatga agggttcttt gacatcgaaa caccagtact aacgaagtca acacctgagg
     6061 gtgcacgtga ctatttagta ccatctcgtg ttcatgatgg tgaattttat gcattaccac
     6121 aatcaccaca attatttaag caattattga tgattagtgg atttgacaaa tactaccaaa
     6181 tcgtaaaatg cttccgtgac gaagatttac gtgcagatcg tcaacctgaa tttacacaag
     6241 tcgatattga aatgagtttt gtagaccaag aagatgtgat gcaaatgggt gaagaaatgc
     6301 ttaaaaaagt tgttaaagaa gttaaaggcg ttgaaattaa tggcgctttc ccacgcatga
     6361 catataaaga agcgatgcgt cgctatggtt ctgataaacc agatacacgt tttgaaatgg
     6421 aattaattga cgtttctcaa ttaggacgtg atatggactt taaagtattt aaagatactg
     6481 ttgaaaatga tggtgaaatt aaagcaattg tcgctaaagg tgcagctgaa caatatactc
     6541 gtaaagatat ggatgcttta acagaatttg taaacatcta tggtgctaaa ggattagcgt
     6601 gggttaaagt tgtggaagat ggtttgacag gtccaattgg acgtttcttt gaaacagaaa
     6661 atgttgaaac attacttaca ttaactggtg ctgaagctgg tgacttagta atgtttgttg
     6721 cagacaaacc aaatgtcgtt gcacaaagtt taggtgcatt acgtgtcaaa ttagctaaag
     6781 aattaggttt aatcgatgaa acaaaattaa acttcttatg ggtgacagat tggccattat
     6841 tagaatatga tgaagatgcg aaacgttacg ttgcagcaca tcatccattt acatctccaa
     6901 aagaagctga tattgctaag cttggcactg cgccagaaga agctgaggca aatgcttatg
     6961 acatagtatt aaatggttat gaattaggtg gcggttcaat cagaattcat gatggtgagt
     7021 tacaagaaaa aatgttcgaa gttcttggat ttactaaaga acaagcacaa gaacagttcg
     7081 gcttcttact agatgcattt aaatatggtg caccaccaca tggcggtatt gcattaggat
     7141 tagaccgatt agtcatgtta ttgactaata gaacaaactt gagagataca atcgcattcc
     7201 ctaaaacagc atctgctaca tgtttattaa cgaatgcgcc tggtgaagtt tctgataaac
     7261 aattagaaga actttctttg cgaattcgtc actaaatttt aaaaaatcaa taaactttgg
     7321 tttgttataa tttcattaat tttaattatg tggtaatata acatcataag atagtcatct
     7381 gagttgttcg taagtttgct ttttaatttg ggcctaacac tctttgatca agggagccca
     7441 atgggttttc ttgcagcgta cacgccttat atgaaggacg tacaaaacaa gaaacagggc
     7501 acccacctgt atataacagg ccgaatgatc aagctattat aactacggca atacggactc
     7561 tatcgatacg caagactttg tcttgcgtat ttttatgcgt ttctttaagt ttttaataaa
     7621 gaattagttg ggttttatag cgtttaataa atgtttcctc gttgaatttt gggaaaataa
     7681 tcaaagtgat aatgctaaat ataaaatttt aagttactat gtacttatgt ataaatcagt
     7741 gaaagaggta aaataatgaa acatcaattt tcaagaaatg aactagcgat tggacaacaa
     7801 ggtttagatt tattgaagca acaaactgtt gtcatacttg gcgttggagg tgttggttca
     7861 tttgctgctg aagcattagc aagaacgaat attggtcata ttatattaat agataaagat
     7921 gatgttgata ttacaaatgt aaacagacaa ttacacgctt taactacaac ggtaggacaa
     7981 agtaaagttt ctttgatgga agaacgtatt aaattaatta atccagattg taaagtcacg
     8041 cccttacata tgttttatac agaagagaca tatgaagaaa ttttcgataa ctacgatata
     8101 gattattttg tagatgcaag tgatacaata atgtacaaag tacacttaat gaaagagtgt
     8161 cttgaccgag ggattaaagt gatttccagt atgggtgctg caaataaaac agatccaaca
     8221 cgttttacaa tttcagatat ctctaaaaca cataccgatc caatggccaa aattattcgt
     8281 caaaaattga aaaaactagg aattcgtaaa gggattccag ttgtattttc agatgaaagt
     8341 ccaattgtca taagagaaga tgtaaaagat atagttggag ataaaaatgc tatcaatcga
     8401 aaaggacaaa tgccaccttc ttcaaatgcc tttgtgccaa gtgttgttgg attaatttgt
     8461 gcaagttatg tggtgaatga cgtattaaaa gatattccag ttcgtcgcat taaagacaaa
     8521 gggcaataat tcattttgaa agggataatt ttcaacgtaa ggcaagtgta accacaccat
     8581 aaaactaaaa ctgactagtt cgcaagcata gtagaatatg cctcgtgtac tagtcagttt
     8641 tgatttgatt atagctaaaa tatcaaaggt aatcgattta atcttatttt tacggccttt
     8701 gtttaagtaa gttgttatat atttctttga gttgttgttc acttttagat gtcgtttttg
     8761 gttcgtaata aattttgttt tttagtttat caggtaaata ttgctgtgaa acatagccat
     8821 taacatattg gtgtggatat ttgtaaccaa tagatcggcc tagatcttta gcaccttgat
     8881 aatgtccatc ttttaaatgg tttggaattt ggcccacatg accgtttcta atatcggaca
     8941 atgcactatc aatggcactc attgctgaat ttgacttagg tgataagcat agttcgatta
     9001 ctgcttggct tagtggaatt ctagcttctg gtaaacctag acgttctgct gattcaatag
     9061 cagcaagtgt tctctgacca gcattaggtg aggctaagcc tatatcctca tagctaatta
     9121 caagtaatcg tcgaactatt gtaggtaaat ctccagcttc aattaatcgt gctaaataat
     9181 gtaaagcggc attgacgtcg ctaccacgga tagatttttg gaaagcgctc ataacatcgt
     9241 aatgcatgtc accatccttg tcacttacaa atgcaccttt ttgtaaacag tctttagcat
     9301 cttgcaatgt aacatgtcga taaccgtctt tgtcattatc tgcgcttaat acagctaatt
     9361 ccaatgcatt taacgcacta cgaacatcac cttggctttg tgtagaaaag taggtcatgg
     9421 catcttcatc aattttgggt tgatatgttt tcaaaccatt ctcatcatct tctattgcac
     9481 gagttaacgc ttggcgcaca tcttcgtcat ttaaaggata taactcgaaa atttgcgctc
     9541 ttgaacgaat cgctggattg atagcatgat aaggatttga agttgtagca ccgatcaaga
     9601 cgattttgcc attttctaaa tgaggtaata aaaagtcttg tttagcttta tctagtcgat
     9661 gtatttcatc taataacaag ataacttgac cagacatttt agcttcttca acaacaagtt
     9721 gcatatcttt tttagtgtta gttacagcat tcaattgtct gaatttatat tgcgtactgc
     9781 ccgaaattgc tttggcaata cttgttttgc ctataccagg tggaccataa aaaatcattg
     9841 aagttaattt ttttgtatca accattcttc tgataatgcc tcttggtcca actaaatgtt
     9901 gttgggaaat gatttcatct atattttttg ggcgcattct cgatgctaat ggttctgtac
     9961 tcacatttgt cacacctttc ttatactatc ttaactcaaa aaatgctaaa atgggaatat
    10021 caatattcga attaagtgag gttatacaat gaaaatttct actaaaggga gatatggact
    10081 tacattgatg atttctcttg ctaaaaaaga ggggcaagga tgtatatcat taaagtcaat
    10141 tgctgaagaa aataatttga gtgatttata tttagaacag cttgtaggtc ctttaagaaa
    10201 tgcggggtta attcgaagtg tacgcggtgc taaaggtgga taccaattaa gagtgccagc
    10261 ggaagaaatc tcagcagggg atattataag actgttagaa ggtccaatta catttgttga
    10321 aagtattgaa tcagaaccac ctgcgcaaaa acaactatgg attcgcatga gagatgcagt
    10381 gagagatgtt ttagataata caacattgaa atatttagcg gaatatgtag atacaagtga
    10441 agatttagac ggatacatgt tttatattta atcgtataaa tactatacta taacataaaa
    10501 acttcatatt ataatgttta gcgaacctcc ttagtggtat ataaatatat acatccaagg
    10561 aggtttcact atggcagacg aaagtaagtt cgatcaattt aaaggaaatg ttaaagaaac
    10621 agtaggtaac gttactgata acaaagaatt agaaaaagaa ggtcagcaag ataaagtgat
    10681 tggtaaagca aaagaagttg ttgaaaatgc taaaaacaaa ataactgatg caattgataa
    10741 acttaaaaaa taagttcaat taaactaatc aaatataaga ggagcgatta atatgagttt
    10801 tatggataaa gctaaagacg cagtagaaaa atttaaaaat agcgataatg aacaagttaa
    10861 aaatgtaaaa gataagataa atgaatatac aggatcgaat aacgaagaga aaaaagaaaa
    10921 tgaagataaa gagaaataaa tttctttaaa ttttggatat gcttttcaat attttgaaag
    10981 cgccccatca aata
//
LOCUS       DAKTIP010000080        10952 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAKTIP010000080 DAKTIP010000000
VERSION     DAKTIP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.080"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..477)
                     /locus_tag="O0305_001511"
     CDS             complement(<1..477)
                     /locus_tag="O0305_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0274658.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFY"
     gene            complement(876..1298)
                     /locus_tag="O0305_001512"
     CDS             complement(876..1298)
                     /locus_tag="O0305_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCY0274659.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            1446..2162
                     /locus_tag="O0305_001513"
     CDS             1446..2162
                     /locus_tag="O0305_001513"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCY0274660.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     VPQAPGQIDVTDLKAQVTLY"
     gene            2245..2784
                     /locus_tag="O0305_001514"
     CDS             2245..2784
                     /locus_tag="O0305_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCY0274661.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEGDMPKAPRKNRNLITLY"
     gene            complement(2934..3254)
                     /locus_tag="O0305_001515"
     CDS             complement(2934..3254)
                     /locus_tag="O0305_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0274662.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            3398..3754
                     /locus_tag="O0305_001516"
     CDS             3398..3754
                     /locus_tag="O0305_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCY0274663.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3912..4292
                     /gene="gcvH"
                     /locus_tag="O0305_001517"
     CDS             3912..4292
                     /gene="gcvH"
                     /locus_tag="O0305_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCY0274664.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVAIIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSEMIGE"
     gene            4471..5349
                     /locus_tag="O0305_001518"
     CDS             4471..5349
                     /locus_tag="O0305_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCY0274665.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMQ
                     EETLNQSKIGGMPFLKYFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            6091..6477
                     /locus_tag="O0305_001519"
     CDS             6091..6477
                     /locus_tag="O0305_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCY0274666.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            6470..6766
                     /locus_tag="O0305_001520"
     CDS             6470..6766
                     /locus_tag="O0305_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0274667.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      6824..6927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7016..8041
                     /locus_tag="O0305_001521"
     CDS             7016..8041
                     /locus_tag="O0305_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCY0274668.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            8034..8729
                     /locus_tag="O0305_001522"
     CDS             8034..8729
                     /locus_tag="O0305_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274669.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            8747..9568
                     /locus_tag="O0305_001523"
     CDS             8747..9568
                     /locus_tag="O0305_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0274670.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            complement(9711..10931)
                     /locus_tag="O0305_001524"
     CDS             complement(9711..10931)
                     /locus_tag="O0305_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0274671.1"
                     /translation="MWLEKFKNKNKETKYRYYEKYKDPLTAKWRRVSVVLNKNGKQSQ
                     KEAQRLLNERIEEKLNDKKPTTLKSLTFHVACDEWFQNYIKTSGSKRTTIKTKLSKLN
                     TLKKFVDEDILINKITLSYAQQVFDEMDSKGYVYQVNKDALSIFKNVFEYTRRIYKLQ
                     DLEFLKDITLNKRIKSYDEVKAKRNKYLELNEIESIIKDINTKAQKMHSGIHKRFYLF
                     VALMTEFQALNGMRIGEMLAIQNEDIDFDNKSLNINGTIHWFHDESGGFGVKDTTKTE
                     SSYRTIGLSSRSCEILKKAILENKKDSKWNDGYLNRNFVFTNHKGNPMQTERFNKILR
                     EAAKDVGIDKEVSSHILRHSHISLLSQQGVSLKAIMDRVGHSDHRTTLSIYSHVTEQM
                     DKDMMNKLEQVKLG"
BASE COUNT         3789 a         1569 c         1922 g         3672 t
ORIGIN      
        1 ataaaaaagc caatgttcat cgaaataggt atttccgatt ttaattgcat ttttctcaaa
       61 accaagaatg tcgaatccta gtgcactata gaaaacttta gcacctatgt tgtttgacac
      121 aattgatgta agtaaagatt cgtaattgtt ctgccgtgca taattaataa tgaaattaat
      181 gagctcgcta ttaatcgatt tatcgttatt agtaacaaag ttgtatttaa ttaatgattt
      241 atgttctttt ccaacgtatc gaatttgttc taaggcagct gttgcgataa gtgtttcgtt
      301 ttcataacag ccaaatacaa tacatttcgg tgaaccttga tttaaaatgt catgtattaa
      361 ggtgtcagtc attgtttgac tataatgtga atcttgagtg aattcttcat tgactgtcga
      421 aacaagtctc ttatactcat taaaatcatt attcgttaat gttttaattt caatcattac
      481 tgacctcctt aatcattatt aagaaagatt ttgtactaaa aaaatttctt aattacatat
      541 taaaatgttt attaaaattt tgcaatcact gatttatatg cacattcttg taaatggatt
      601 atcaatttaa ttatatttat ttttaagttc aggaaattat ggaattggtt attggtcgac
      661 actggacttt tatttgtaga cggtatgcga agaagtcaag ccagtattgg aaccggtcac
      721 atttatgatg actaaggtac aatcattttc agttaaatat tgccagaaat ttcaatcacg
      781 aatagaaaaa ataaccaacc aaaaattttt ggttggcaaa tttgaatttt atgtgaattt
      841 tattaataac gttgaataat ctctttaagt taatgctaat ctacaacatt tacatttaaa
      901 tcgacattaa tatttccttg agtcgctttt gaatatggac aaaattcatg agccatttgt
      961 aaatattttt cagcttcttc ttgagatata acatttttaa ttgtcgcatc aattgaaaca
     1021 cttaatttag gactttctga gtctgaatca tcttctagtc tcactgttag tgttacttct
     1081 ggatgagcat cacgcacttt gttttgcttt aaaattaggt cgaaagcacc gttgaagcaa
     1141 gatgcataac ctgctgcaaa taattgttcg gggttagtag ctttaccatc tgcttgagca
     1201 ggcggaacga tatcaatatc taatgctcga tcatcagtat aaacatgtcc tttacgtccg
     1261 ccaacattcg tcgctttagt ttcataatgt attgccatac ttataacctc ctaatttaaa
     1321 atacatttat gctttaccca ttcgaaaata ccttaatcat tttcatttat agcattgttt
     1381 gattgaagga taaaaagttg ttgtttacaa taaaaataat gagtatctga aatgagggat
     1441 tcactatgac acatgtggaa gtagtagcga ctatcgcgcc acaattatct atcgaagaaa
     1501 ctttaattca aaaaattaat catcgtattg atgcaataga cgtattagaa ttacgaattg
     1561 atcaaattga aaatgtcaca gttgatcaag tggcagaaat gattacaaag ctgaaggtta
     1621 tgcaagattc attcaaatta ttagttacgt atcgtacaaa gttacaaggt ggctatgggc
     1681 aatttacaaa tgactcgtat cttaatttaa tatcagactt agcaaatatc aatggcatag
     1741 atatgattga tatagaatgg caagcagata ttgacattga aaaacatcaa cgaatcatta
     1801 cacatttgca acagtataat aaagaggtgg ttatatcaca tcataatttc gaaagtacgc
     1861 ctccattaga tgaattgcaa tttatatttt ttaaaatgca aaaattcaac ccagaatacg
     1921 ttaaattagc agtaatgcca cataataaaa atgatgtgtt aaatttattg caggcaatgt
     1981 ctacattttc agatactatg gactgcaaag ttgttggtat ttcaatgtct aaacttggac
     2041 taataagtag aacggctcaa ggcgtttttg gtggtgcgtt gacttatggt tgtatcggag
     2101 taccacaagc tccaggacag attgatgtta ctgatttaaa agcacaagtg actttatact
     2161 aataaaatga attaggttgc ttttgtgtaa gtgctgtctt ataattgaga agtattatca
     2221 ttgttcgtgg aggagcgatt ttagatggaa ttacaacaag caatagctaa tagaagaagt
     2281 gtgaaaaaat ttaaaagaga tatgcacata gatgacgcat tgctatatca agcaattgag
     2341 aaagctgctg atgctccaaa tcacggaatg agggaaccat ggagagttgt gcatgttccg
     2401 aaagacagat taggagatat gagtaaggat atttctaaat ttgcatttcc taatgaatta
     2461 gataagcaac aatgtcatta tgatgcagtt acgaaactag gtggcatgtt attgcttatt
     2521 ttaaaaacag atccaagaca acgtcaaaat gatgaaaact actttgcatt tggtgcatat
     2581 gcacaaaatc ttatgttgtt actttatgaa gcgggaatag gtacatgttg gaaatcgcca
     2641 ttatatatct atgatcctaa agtaagaaaa acacttggta taaagaaaga tgaagttctt
     2701 gctggattct tatatttaac ggatttagaa ggagatatgc ctaaagcacc acgtaaaaat
     2761 agaaacttaa ttacattata ttaatatgta taattataga aacattaata aaagctgagt
     2821 catgaattga tggacatcta tcgagttaga gatttaatct aacttactag agtcggtaca
     2881 ataacagtct cagcttttta ttgtgcagta tatacacatt tttattttag tatttattta
     2941 aaagtttctg ctaaaaatga ttcaacttgt tcaggtgact tagcatttgc tgaatgaagg
     3001 tgtgcaattt tatcgccgtt tttaaatact agcaagctag ggatacccat aacttcattt
     3061 tcaacaacta catcttctaa ttcatcacga ttaacagtat accattggta atcattatat
     3121 tgttctacga ttgggtcaat ccataaatcc atagcacgac agtctgggca ccatcctgcc
     3181 tcaaatttaa caattacagg tgtatcgcta ttaattacag atttaaatga ttcattactt
     3241 ttgattgatt gcattgtaac aactcctcta gatagtttaa taatttttat tatagctaaa
     3301 tttatatcat aataaaaaat tttagcttca aaatgaaagc cttttgcttt ggaaaatgat
     3361 atatttattt taaatacata aaggaggttg cagtcgtatg attaaatttt accaatataa
     3421 gaattgtaca acttgtaaaa aggcagcaaa gtttttagat gaatatggcg taagttatga
     3481 accaattgat atcgttcaac atacacctac aataaatgaa tttaaaacaa taattgcaaa
     3541 tacaggcgta gaaattaata aattgtttaa tacacacggc gcgaaatatc gtgagcttga
     3601 tttgaaaaat aaattacaaa ctttatcaga tgatgaaaag ttagagttgt tatcatctga
     3661 tggtatgtta gtaaagcgtc ctctagcagt aatgggcgat aagataacat taggatttaa
     3721 agaagatcaa tataaagaga cttggttagc gtaagtgaaa tgtaagcgtt tactaaatat
     3781 ctcgatattt agattcatta catgtaaaat gaaataagct atacaattgt taatttttat
     3841 aaatatagtt gaatagcatc tagccttatg gcatcattaa tgatgtaaag attaattagg
     3901 aggggattct cttggcagta ccaaatgaat tgaaatattc aaaagagcat gaatgggtta
     3961 aagttgaagg taatgtagca ataattggaa tcacagaata cgcacaaagc gagttaggtg
     4021 atattgtttt cgttgaatta ccagaaacag atgatgaaat taatgaaggg gatacgtttg
     4081 gtagcgtaga atcagttaaa actgtatcag aattatatgc accaatctct ggtaaagtag
     4141 ttgaagtcaa cgaagaacta gaagatagtc ccgaatttgt aaatgaatct ccatacgaaa
     4201 aagcatggat ggtaaaagta gaaattagtg atgaaagtca gattgaagct ttattaacag
     4261 ctgaaaaata ttcagaaatg attggtgaat aatcaccgtg taactcctta atctaagatt
     4321 gaggagttta tttttagtct gagaaaataa atgatatgaa agaaaaatta ttaggtacta
     4381 ttatttggag tattgctaca ttttattatt caagaatgat ggaaataatg aatttagcta
     4441 ttttaaaaat aaaaattggg ggaagttaat atgctaaaca ttcaagacgt tagtcatctt
     4501 tctaaaaagg agcaaaaagc atataaccgt ttcgtagaat ctgtagaaaa cggtaattta
     4561 ccagtactac catgtattga aatggatcta aaagagatgc aagaagaaac attaaaccag
     4621 agtaagattg gtggaatgcc atttttaaaa tattttaaag atataccatt agatgaaaat
     4681 aatgtaccaa tggtattgct agcacagatt aatttggatg atcttccaga acaacaagaa
     4741 ttatttcctg taaaagaagg gatattgcag ttttggatta gttcagaaga tcaaatgtat
     4801 ggaatgtctg aaaatttaaa gggaaacaat ataaactcaa ggcttgttta tataaaagag
     4861 ccaattacag atttatcact cgaaaatatt caagcgcatt tgaagtcatt agatgctgat
     4921 aatgaggata tcccgttcag tggagcattt tctatagaat ttagattgtc gaaacaaact
     4981 attacatgta ctgattataa gtacgatgag gacgtgcttg cattgtggaa taaagtcaat
     5041 ccatccttcg cgctaaaatc aatgtttggt ggttatgatg aattgatgga acctgtgtgt
     5101 aacacattta ctgctaagga accatttaat caacttggtg gttatccata ttttgaccaa
     5161 atagatccaa gaacgaacga tcaagaactg aaaatgtatg atagagtctt actgcaaatt
     5221 gattctacaa gagatggtaa ttcttcgatt atatggggtg atttaggtat tgccaatatc
     5281 ttagtgaaat ctactgacct tgaggctatg aagtttgatg attacatgta ttcatgggat
     5341 tgcagctaag agaatcataa aaatatatta ctttaataag tgtagtatct atagatcaat
     5401 ctagggagtg ggacataaat gatattttcg caaaatttat ttcgtcgtcc caccccaact
     5461 tgcattgtct gtagaaattg ggaatccaat ttctctttgt tggggcccat ccccaacttg
     5521 cattgcctgt agaatttctt ttcgaaattc tctgtgttgg ggcccctgac tagaattgaa
     5581 aaaagcttgt tacaagcgca ttttcgttca gtcaactact gccaatataa cttcgtagag
     5641 catagaacat tgatttatgt cccagcctgt tttcataagc cacagctaat gtgtatttaa
     5701 aaataggaat acatgagtaa aactcatgca taagaaatac taatttctaa agaaaaagta
     5761 tttctttatg ttggggcccc gccaacttgc acattattgt aagctgactt accatcagct
     5821 tctgtgttgg ggccccgcta taattgaaaa gtttgttata ggtgtatttt cttttggtta
     5881 actattgtta atataacatt gtagatttta ggatgttgat ttttgactta gcctttgtct
     5941 gcttatgtta aatgttccag ctagtcaaac attgagtttg taattattat attaagctag
     6001 tttaaatttt ttgtgattta tgttttattc ataccaattt tgtgaaatga tacagtgaca
     6061 gatgtttatt gattttaaag ggtgatgatg atggctattg taaataaagt gataattgtt
     6121 gaaggaaaat ctgataaaaa aagggtgcaa caggttattg cagaaccagt caatattatt
     6181 tgtactcatg gaacaatgag tatagataag cttgatgata tgatagaatc actgtatgat
     6241 aaacaagttt ttgtattagc cgattctgat gacgaaggag atcgaattag aaattggttt
     6301 aaacgttatt tgagtgaaag tgaacatata tttattgata aaacttactg tcaagttgcg
     6361 aattgcccca aacaatattt ggcgcatgta ctttcaaaac atggctttac ttgtaagaaa
     6421 gaaacacctc ttttaccgaa tataaataat gaaaggttag ttttagtaaa tgaataattc
     6481 attagacatc aaagatgtaa ctacatttta tgaggaagac aaacatttaa tctttggtta
     6541 tacaccaacg tgtggtactt gtaaggtttc agaaagaatg ttagacattg ctaatgaaat
     6601 attgcagtta ccattattga aaatagattt aaacttttat cctcagtttt gtaaagatat
     6661 gcaaatcatg tctacgccga ttttattgtt gatgaataaa gataaagaag taaaacgaat
     6721 ttatgcattt aaatcggtga ctgatttgtt agaaaattta aaatagttat tgacgaaatt
     6781 ttactaaggt gttaggatta atacaaatta aatgatgcgt aaactcttat cgagagtggt
     6841 ggagggatgt gccctacgaa gcccggcaac cgtcttatat agaaatggtg ccaattcaca
     6901 taaagtttta acttttgaag atgagagaaa caatactact attgctttct caatttttct
     6961 atcgatattg agaaagcatt ttttatttta ttaagcaaca cagggaggaa tcaacgtgat
     7021 tgaattaaaa gaagttgtta aagaatatcg gactaaaaat aaagaagtcc ttgctgtaga
     7081 tcacgttaat ttatcgattc gagcaggatc gatttatggc gtcattggtt tttctggagc
     7141 aggaaaaagt actttgattc gaatgtttaa tcatttagaa gcgcctacat caggtgaagt
     7201 tattatagat ggagaccata taggtcaatt gtccaaaaat ggattaagag caaaaagaca
     7261 aaaagtaagt atgatcttcc aacattttaa tttgttatgg tcaaggactg tgttaaaaaa
     7321 tattatgttt ccgcttgaaa ttgcaggtgt ccctagaagg agagctaagc aaaaagcatt
     7381 agaacttgtc gaactcgtcg gtttaaaagg tagagaaaag gcttatccat cagagttatc
     7441 aggtggacaa aagcaacgtg ttgggattgc acgagcgtta gctaatgatc caacggtctt
     7501 gctttgtgat gaggcaacaa gtgcacttga tccgcaaaca acagatgaaa ttttagatct
     7561 actactaaaa attagagaac aacaaaattt aacaattgta ctaattacgc atgaaatgca
     7621 tgtcattcgt cgtatttgtg atgaagttgc agttatggaa agtggtaaag tgatagaaca
     7681 aggaccggtg acacaggttt ttgaaaatcc gcaacacact gtgacaaaac gatttgtgaa
     7741 agacgattta aatgatgatt tcgaaacatc tttaacagaa ttagagccat tagaaaaaga
     7801 tgcatatatc gttagattag ttttcgctgg ttcaacaaca accgagccta ttgtatcgag
     7861 tctatcaact gcctatgata ttaaaattaa tattttagaa gcaaatatta aaaatacaaa
     7921 aaatggaaca gtcggctttt tagttctgca tattccatat atttcaagtg tagatttcgg
     7981 aaaattcgaa aaagagttaa ttgagcgaca agttaaaatg gaggtgttaa gacatgggta
     8041 aatcatttag tgaaattata aatgaaatga ttacaatgcc taatattcag tggccagaag
     8101 tttggactgc aatagtcgaa acactataca tgacagtcgt ctcaactata tttgcattta
     8161 tacttggtct tattttaggt gtgttattat tcttgtctgc taaaggtaag tctatcggtg
     8221 caaggttatt ttattctatc gtttctttca ttgttaactt atttagagcg ataccattta
     8281 ttattttaat tttattatta attccattta caagtttgat acttggaacg ataagtggtc
     8341 cgacaggtgc gttaccagcc ttgatcattg gcgcagcacc gttttatgca aggctcgtag
     8401 aaattgcttt taaagaaatt gataaaggtg tcatcgaagc ggcttggtca atgggcgcta
     8461 atacttggac agtaattcgt aaagtccttt tacctgaagc tatgccagcg ctagtgtctg
     8521 gcattacagt tacagcaatc gctttagttg gttcaacagc agttgcaggt gtaattggtg
     8581 ccggtggttt aggaaattta gcatacttaa caggtttcac tcgaaatcaa aatgatgtca
     8641 ttttagtatc aacagttttt attttaatta ttgtatttat aatccaattc attggggatt
     8701 ggcttacaaa taaacttgat aaacgataaa ttgggggttt cattttatga aaaaattatt
     8761 tggtcttatt ttagtattaa catttgcagt tgtattagca gcttgcggta atggaaacaa
     8821 aagtggcagt gacgacaaga aaataacagt aggtgcttca ccagcaccac atgctgaaat
     8881 tttagaaaaa gcaaaaccat tattagagaa aaaaggttat gaactagata ttaaaacaat
     8941 taacgattac actacaccta ataaattact agacaaaggt gaaattgacg caaactattt
     9001 ccaacataca ccatatttaa acacagagaa aaaggataaa ggttacaaaa tcgtaagtgc
     9061 cggtgatgtt cacttagaac ctatggctgt atactctaaa aagtataaaa gtttaaaaga
     9121 attaccaaaa ggtgcaacag tctatgtgtc taataatcca gctgaacaag gacgtttctt
     9181 aaaattcttc gttgatgcag gtttaattaa aatcaaaaaa ggcgtaaaaa ttgaagatgc
     9241 taagtttagt gatattacag agaataaaaa agatattaag tttaataata aacaatcagc
     9301 agaattctta cctaaaattt atcaaaatga agacgctgat gctgttatca ttaattcgaa
     9361 ctttgcaatc gaacaaaaac taaatcctaa aaaagattct attgctgtag aaagtgcgaa
     9421 agataatcct tatgcaaact taattgctgt taaagaagga catcaagatg ataagaaaat
     9481 caaagcatta attgaagtat tacaatctaa agatattcaa gacttcatta atgaaaaata
     9541 caatggtgca gttattcctg ctaaataata ccaattaaat aaaaataccc tttaataaca
     9601 acgattaagg ggttattttt gtggtattga aaataaaaaa gggcacaaaa ggggcagtat
     9661 atgcaaatta tcgaatttta aacaaaaaaa tacgctcaga tttgagcgca ttatccaagt
     9721 tttacctgtt caagtttgtt catcatatcc ttatccatct gttcagtaac gtgagaataa
     9781 atggaaagtg ttgtacggtg gtcggaatgg cctacacgat ccataatagc tttaagtgac
     9841 acgccttgtt gagagagtaa tgatatgtgg ctatgtctta atatatgtga agaaacttct
     9901 ttatcaatac caacatcttt agctgcttcc ctaaggattt tattgaatct ttcagtctgc
     9961 attgggttgc ctttatgatt agtaaataca aaatttctat ttagatatcc atcattccat
    10021 tttgaatcct ttttgttctc cagtattgct ttctttaata tctcgcaact tctgctactc
    10081 aatccaattg ttctataact agactctgtt ttagtggtat cttttacacc gaatccacca
    10141 gattcatcgt gaaaccaatg gattgttccg ttaatattta aactcttatt atcaaagtct
    10201 atatcttcat tttgaatggc taacatttca ccaatacgca taccatttaa ggcttggaat
    10261 tctgtcatga gtgcaacgaa taaataaaac cgcttatgga tacctgagtg catcttctga
    10321 gccttcgtat taatatcttt gatgatagat tctatttcat ttaattcgag gtacttatta
    10381 cgtttagctt tcacttcatc ataagactta attcttttat ttaatgttat atcttttaaa
    10441 aattctaaat cttgcagttt atagatacgt ctagtgtatt caaatacatt tttgaatatg
    10501 cttaacgcat ctttgtttac ttgatataca taacctttac tatccatttc atcgaaaact
    10561 tgttgcgcat atgaaagtgt tattttattg attaaaatat cttcatctac aaatttcttc
    10621 aaagtgttta gcttactcaa tttagtttta atagttgttc tttttgaccc agacgttttg
    10681 atataattct gaaaccactc atcacatgcg acatggaaag ttagtgactt aagtgtagta
    10741 ggtttcttat cattcagttt ttcttctata cgctcattta agagtctttg agcttctttt
    10801 tgtgattgct taccattctt attaagtacc acgctaacac gtctccattt ggcagtgaga
    10861 gggtctttgt acttctcgta atatctatat ttagtttctt tattcttatt tttaaatttt
    10921 tctaaccaca ttttgcttct ccctcctcaa aa
//
LOCUS       DAKTIP010000081        10944 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAKTIP010000081 DAKTIP010000000
VERSION     DAKTIP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.081"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(115..858)
                     /locus_tag="O0305_001525"
     CDS             complement(115..858)
                     /locus_tag="O0305_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0274672.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            938..1384
                     /locus_tag="O0305_001526"
     CDS             938..1384
                     /locus_tag="O0305_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCY0274673.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            1498..2658
                     /locus_tag="O0305_001527"
     CDS             1498..2658
                     /locus_tag="O0305_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCY0274674.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            2645..4249
                     /locus_tag="O0305_001528"
     CDS             2645..4249
                     /locus_tag="O0305_001528"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0274675.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHTLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAV
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            4343..4438
                     /locus_tag="O0305_001529"
     CDS             4343..4438
                     /locus_tag="O0305_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274676.1"
                     /translation="MKPLVQSFTTSYAVKQQSNYKLSSTPLSSPK"
     gene            complement(4399..5529)
                     /locus_tag="O0305_001530"
     CDS             complement(4399..5529)
                     /locus_tag="O0305_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0274677.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(5636..7102)
                     /locus_tag="O0305_001531"
     CDS             complement(5636..7102)
                     /locus_tag="O0305_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCY0274678.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDK
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            complement(7230..7847)
                     /locus_tag="O0305_001532"
     CDS             complement(7230..7847)
                     /locus_tag="O0305_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCY0274679.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            8019..9293
                     /locus_tag="O0305_001533"
     CDS             8019..9293
                     /locus_tag="O0305_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCY0274680.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            complement(9387..10649)
                     /locus_tag="O0305_001534"
     CDS             complement(9387..10649)
                     /locus_tag="O0305_001534"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCY0274681.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
BASE COUNT         3761 a         1956 c         1646 g         3581 t
ORIGIN      
        1 agaatttaca agtcttattt gtttaattat ctttagtatt tcatcattag tttttaaaat
       61 tatcaattgc gatatacgaa tcctcatccc tctatcaaat taccattcat cgtctcactt
      121 aataaataat ttcggattat ctgtaattaa tccatctaca cccatttgtc ttaatttttc
      181 tccagttttc aatttattaa ctgtgtatgg cataacctgt aattgatgat gatgtgcttt
      241 atcaacaaat tttctggtca ctaatgcata attaggatta acataactag caatttgagc
      301 aattcttgaa aagtttggct ttttatacca ataattacgt ttactacaaa gcacacctaa
      361 ttcatatata ctgcccaatg tgtttaactt ttcgatgcat tcgatatcaa aagattgtat
      421 aactacttgt gtcgcatcga ctttcttttc ttccaaaaat gccagcaatt tacattctat
      481 ttctggatat aaattcggac ttttcaattc aatcaatagc tttttatcat actttaagca
      541 taatgaaagt acttcatcta acgtgggtat tcgttctccc ttaaaagcaa catctttata
      601 actaccaaaa tcaaatgatt ttaattgcga taatgtgtaa tcagcaatac gccccttacc
      661 atccgatgtt ctatcaattg tttcatcatg tatcacaaca aaatgttggt ctttggtcaa
      721 atgaacatct atttctaaca tagcaacatt gagccccatt acctctcgat aaccgaccat
      781 tgtattttca ggaaaatcac tcggcaatcc acggtgcgaa acaatttgta attcatcttt
      841 cagtttattc agagtcattg ataagtcacc tttctgttaa actttgataa actattctaa
      901 aattaaacta tcacaattat attggaggtt aaagcaattg catcaacatg actttaaagt
      961 ccaaacttct tggcaaggtg gtcgtaacaa tgtcggaaac gttcaaggcg acatactttc
     1021 agagaatatt tctatacctg cttctttagg tggtgttggt ataggaacaa atcccgatga
     1081 aatgttagta tcagccgctt catcatgtta tatcatctca ttagcagcta ctcttgaacg
     1141 tgcaaagttc acagatattt caattgaaca acaatcgatt ggaacagctt gtttaaataa
     1201 cggaaaattc agtatgtcaa aaattgtgca ccatcctcaa attcaaattc caagtgatca
     1261 aatagcacaa ttagaaaagc gattaccaaa attgataaca attgcagata ataattgcat
     1321 gatttcaaat gctgtaagaa ataatgtgga cataaaaatc tatcccatca ttcaggccaa
     1381 ataaaatcgt taagtgataa cgtttgttcg acaatatagc gttatcactt ttttaggctt
     1441 ttaaattttc agaaaatttg ctaaaatagt gaaatcttat ttataggagg catataaatg
     1501 tattatcatc aaccgttgtt attaacacct ggcccaaccc ctgtacctga tgccattatg
     1561 agagaaattc aagcacctat ggttggtcat cgttctaaag attttgaaga catcgcacaa
     1621 caagcatttc aaggtctaaa gccaatattt gggagtcaaa atgatgtact tattttaaca
     1681 tctagcggta caagcgtctt ggaggctagt atgttgaaca ttgtaaaccc tgaagatcac
     1741 ttcgttgtca ttgtttcagg tgcctttggt aaccgattta aacaaattgc acaaacttat
     1801 tacaaaaatg tgcatattta tgacgtaaca tggggagaag ctgtagatgt caaagatttc
     1861 atcaatttcc tttcaacttt aaatgttgaa gttaaagcag tatttagtca atattgcgaa
     1921 acatctacga cagtgctaca ccctattcac gagttaggaa atgccattaa tcaatttaat
     1981 agtaatattt attttgtagt tgacggcgta agttgcattg gtgctgttga tgttgacatt
     2041 aacaaagata aaattgatgt acttgtttct ggtagtcaaa aagcaattat gttacctcca
     2101 ggattagctt ttgtagctta tagccaccgt gcaaaagaac atttcaaaga agtaactacg
     2161 ccaaaatttt atctagactt aaataaatac atttcgtcac aagctgacaa ttctacaccg
     2221 ttcacaccaa atgtgtcttt atttagaggt gtaaatgcat acgttgaaac cgtaaaagca
     2281 gaaggtttca atcacgtaat agcacgacac tatgcaatta gaaatgcatt aagaagcgcc
     2341 ttaaaagcat tagatttaac tttattagtc aatgataaag atgcatctcc aacggttaca
     2401 gcattcaaac ctaatacaaa tgatgaagtg aaaataatca aagatgaact taaaaatcgc
     2461 tttaaaataa caattgctgg tggtcaaggc catcttaaag gtcaaatttt aagaattggt
     2521 catatgggga aaattagtcc tttcgatatt ttatcggtag tatctgcttt agaaattatt
     2581 ttaactgaac accgtaaagt taactatatc ggtaaaggta tatcaaaata tatggaggtt
     2641 attcatgaag caatttaatg tactcgttgc agatcccata tcaaaagatg gtatcaaagc
     2701 attattagat cacgaacaat tcaatgtaga tattcaaact ggcttgtccg aagaagcatt
     2761 aatcaaaatt ataccttcat accatgcttt aatcgttcgt agtcaaacta cggttactga
     2821 aaatatcata aatgctgctg attctttaaa agtaatcgca cgcgccggtg ttggtgtaga
     2881 taatattaat attgatgctg caacattaaa aggtatttta gttattaatg ccccagatgg
     2941 taatacgatt tcagctactg aacatacact ggcaatgtta ttatcaatgg cacgaaatat
     3001 tccgcaagca caccaatcac ttacaaataa agaatggaat cgaaatgcat ttaaaggtac
     3061 tgagctttat cataaaacat taggtgtcat tggtgctggt agaattggtt taggtgttgc
     3121 taaacgtgcg caaagtttcg gaatgaaaat actagctttt gacccttact taacggatga
     3181 aaaagcaaaa tctttaagca ttacgaaggc aacagttgat gagattgccc aacattctga
     3241 tttcgttaca ttacatacac cactaacacc taaaacaaaa ggcttaatta atgctgtctt
     3301 ttttgccaaa gcaaaaccta gtttgcaaat aatcaatgtg gcacgtggtg gtattattga
     3361 tgaaaaggcg ctaataaaag cattagacga aggacaaatt agtcgggcag ctatcgatgt
     3421 gtttgaacat gaacctgcaa ctgactcgcc tcttgttgca catgataaaa ttattgttac
     3481 acctcatttg ggtgcttcaa cagtcgaagc tcaagaaaaa gtggcaattt ctgtttcaaa
     3541 tgaaatcatc gaaattttaa ttgatggtac tgtaacgcat gcagtgaatg cacctaaaat
     3601 ggacttaagc aatatagatg atactgtaaa atcattcatc aatttaagcc aaacagttgg
     3661 tgaattagct attcaattaa tgtacaatgc accaagctct attaaaatta cgtacggtgg
     3721 cgacttagcc tctattgata gtagtttatt aacacgtaca attattactc atattttaaa
     3781 agatgatctt ggtcctgaag tcaatattat caatgctcta atgttgttaa atcaacaaca
     3841 agtgacatta aatattgaaa ataataaagc agagacaggt tttagtaact acttagaggt
     3901 agaactatca aacgatagcg attccgttaa agttggcgct tctgtcttta caggtttcgg
     3961 tccaagaatt gttagaatta ataatttttc tgtagactta aagccaaatc aatatcaaat
     4021 tgtgtcatat cataatgata ctccaggtat ggtaggaaaa actggcgcat tgttaggtaa
     4081 atacaatatc aacattgcat ctatgacttt aggtagaact gaagcgggcg gagatgcgct
     4141 aatgatttta tccgttgatc aacctgtttc aaacaatata attgatgaac ttaaacaagt
     4201 tggtgaatac aatcaaattt tcacaactga attgacggta cagtcataaa caatttatgt
     4261 aaattcaaaa tgccaagaag acattggatt acttccattg cttcttggca ttttttcatt
     4321 attaatttca ttgtaactca atatgaaacc tttggttcaa tcattcacta caagttatgc
     4381 tgtcaaacaa caatcaaatt acaaattatc tagtacacct ctaagttcac ctaaatgatt
     4441 aataacatag tcggcatgat gcgcttctaa ctcacctgca gcatccttac cttttaaacc
     4501 tgttaatgtt ccaataaacg ttgcacctat tttttgagca cttaataagt cagctaacga
     4561 atcgcctact ataaatacgt catctttatt tacaatgtta tcttgcttat tgatataaga
     4621 ttcatattta tcgcgattat taccatataa agctgcgata taactaaaag gattcggctt
     4681 tcctaatggt cgtgcttgcg gatacatatt ctctgcttct aaaacatcac ttgctgttgc
     4741 aataaaatca gcttcaaaat atggtaacaa tcctaaattt tcaaatggca caacagtctc
     4801 agtataagga cgacctgttg caatacctaa ttcgaaacca gcacctttta aatcattcag
     4861 aagtacctta acttcatcta ctggtctcaa aataatttct tgataaatat aacctgtctt
     4921 aaaagtagtt cgtgctattt tcttttcaac atcttcatac aacttcgatc ctaaatacca
     4981 ttcttgataa acttcttgcg ctaacgtcca taatgcaccc ttcaaactaa ataaagtagc
     5041 atccgaaaca tgtaactctg ttgttgcaaa ctcttccaaa gcagcataaa tattattttt
     5101 accaactttt acattatcca aaaattgtaa aggtagttgt tcatttaaat taaaacagtc
     5161 tgctaagttt gtgcttatat tttgcaactt caattcgaca ggctcatctt gatacatgaa
     5221 tgcctcaatt tcatcatgtg ataacttttt caaaatatca attaaatgaa tactaaaaac
     5281 gataaataac atatcccaat ttgaattcag ccctagcgat tttaatttgt ttaaaatctt
     5341 atctttttga aaaattcgat tcctaatgtc ttgtatatcg ttatcagtca aagtttccca
     5401 atctatatgt gaatgaagac ctaaataaca cttatccatt aataattcat ataccgttaa
     5461 tgcagagaca tcgaaacaac gttcttcact taaaaaaacg ccatcaacat caaataaaat
     5521 tttcttcaca atccccactc catttctgaa aattcagata taaatcattc tactatttga
     5581 ctaaaaaaag cgcaaaccct attgaagtta gatttgcgct ttagctgttt aaattttata
     5641 aatgtttttc aatttcatca gcaacctgct gtacgtgtgt accgacaata acttgagttg
     5701 aatgtttgcc attaacagta acaccaactg caccggcgtt tttaatcttc tgtttatcaa
     5761 taatagatgt gtcttttaac tctagacgca accttgttgc acaattggtt aaattaacaa
     5821 tattctcttg accgcctaaa ccttctaata tttgtatagc atgttgatga tatttacttt
     5881 gtttaatatc attttcacca ggagcaatat tatcttttac aactgttggg tcaactaatt
     5941 cattttcacc tctaccaatc gtattcaagt taaatacttg gattactaca cggaaaatca
     6001 catagtataa gatgaaaaat acaacacctt gaacaagcaa catcaatgga tgatttgata
     6061 ctggattaat tagtgataac acataatcta tcaaacctgc actaaatgaa aatccagctg
     6121 tccaatggaa tgtagctgcg ataaataaag ataatcctgt taataacgca tgaacaacat
     6181 ataagattgg cgcaacaaac ataaatgcaa actcaatcgg ctctgtaaca ccaacgaaaa
     6241 atgctgcaac tgaactcgct aggaaccaac cgtaaacttg ttttttctga gtagttttag
     6301 ctgtatgata cattgctaac gcagccgctg gaataccgaa catcatgatt gggaagaatc
     6361 ccgcttgata gcgtcctgta atacctttta tagcatcttt gccactttgg aatttaccaa
     6421 tatcattaat accaatcgta tcaaaccaga acacactatt cagtgcatga tgtaatcctg
     6481 taggaattaa taatctattg gcaacaccat atatgaaagc tccaaacgat cctaaaccga
     6541 ctatagattc accaaatttt acaatccatg aataaagtag tggccataag aataacaata
     6601 tgacaactaa aaatgtacag taaaatgcag tcataattgg aactagacgt ttaccactaa
     6661 aaaatgataa tgctaatggt aattctgttt cactaaactt attgtatgca taagctgcta
     6721 ttaaacctat tacaatacca acaaagacat tgccattatt catcttttca aaagctgaat
     6781 ttatttccga agctttcatt cctaataaag gcgctaattt cattggtgat aatacaactg
     6841 taactaaaaa atatcctaac gtagctgcaa gcgcgactac accatcattt ttctttgcca
     6901 ttcctatagc tacaccaatt gcaaataaaa tacctaattg ctctaaaatc gtagtaccta
     6961 ccgtagtaaa gaacattgcg attttcggcg tcgcatgaag tgcatttaac gtattaccaa
     7021 ttccggcaat aattgctgca gccggtaaaa tggcaactgg taacattaac gaacgcccta
     7081 aattttggaa aaatttatac attgaatgtc atccttctta aaataatgta gaaatataaa
     7141 gattactaat gtaactagaa taactacttc gatactccgt tatagtcacc taggcttact
     7201 aaccagctat atttctacct caagttattt tataaacttt ttacaatttc atgcaattct
     7261 tgttgtaact ttgctgttcg tgtttcaatc tcttttgtaa tataatcgat acgctcgttt
     7321 cgttttaaat ctttaggtaa atcgttaata tcgattggtt tgccaatatt tatgtatgct
     7381 tgtcctgtta aaagaccgtg aatcttagta ggaccaacat aagcaacagg taatattggt
     7441 gacttactta acattgcaat tgttgaagca ccacgtttca aaggtgcacc ttcttgcgat
     7501 gtgcgagaac ctgttgggaa gataccaact gtcttattat ctttcaacaa attgattggg
     7561 cgttttaaag tactaggtcc tggattttca cgatctacag gaaatgcatt taaagacgtt
     7621 aaaaatttac caatccattt atttttgaat aattcttttt tagccatata atgaatttga
     7681 ttaggatata atgccatacc tagcataatg acttcgttat aactttcatg cgtacaagtt
     7741 acgacatatt tactatcctt aggaatatta tctttaccga ttacgtataa tgattttgac
     7801 attttaacta aaatgaaatt caaaatctta ctaatcactg aatacattgt gccacctact
     7861 taacttattt gtttataaca actagatttt atcactatat ttttactaga acaagatagt
     7921 ctgaaaatac atctaaagtt gtatagtgta ttactagcaa taattataaa aaaaagacat
     7981 aattaaagca tatataaatc atataatgga ggttaagtat gtcagatttt aatcatacag
     8041 atcattctac aacaaaccat agccaaacac ctagatacag aagacctaaa tttccatggt
     8101 ttaaaacagt catcgttgca ttgattgctg gaattattgg tgcacttcta gtacttggta
     8161 taggcaaagt attaaatagt acaattttaa ataaagatgg ttcaactgtt cagacaacaa
     8221 ataataaagg tggcaatcaa ttagacggtc aaagcaagaa attcggtacc gttcatgaaa
     8281 tgataaaatc tgtctcccct acaattgttg gagttattaa catgcaaaaa gcatcaagtg
     8341 tagacgactt attaaaaggc aaatcatcta aaccatctga agctggagta ggttcaggtg
     8401 ttatctatca aataaacaac aattcagctt atatcgttac aaacaatcat gttattgatg
     8461 gcgcaaatga aattagagtc caattacata ataaaaaaca agttaaagcg aaattagttg
     8521 gtaaagatgc agtaactgat attgctgtac ttaaaattga aaatacaaaa ggtattaaag
     8581 cgattcaatt tgccaactct tcaaaagtac aaactggcga tagcgtattc gcaatgggta
     8641 acccattagg attacaattt gctaactctg taacatctgg tatcatttca gcaagcgaac
     8701 gtacgattga cgctgagaca actggtggca atacaaaagt tagcgttctt caaacagatg
     8761 ctgctattaa cccaggtaac tcaggtggcg cattagtaga tattaatggt aatttagttg
     8821 gtattaactc aatgaaaatt gctgcgacac aagttgaagg tatcgggttt gctattccaa
     8881 gtaatgaagt taaagtaaca attgaacaac ttgtaaaaca tggtaaaatt gaccgccctt
     8941 cgattggtat tggtttaatt aatttgaaag atattcctga agaagagcgc gagcaacttc
     9001 atactgatag agaagacggt atttatgtcg ccaaagctga tagtgatatt gatcttaaaa
     9061 aaggtgatat tattacagaa attgatggca agaaaattaa agatgatgtt gatttaagaa
     9121 gctatttata tgaaaataaa aaacctggtg aatcagtcac tgttaccgtt atccgtgatg
     9181 gtaaaacaaa agaagttaaa gtgaaattaa aacaacaaaa agaacaacca aaacgtcaaa
     9241 gccgatcaga acgtcaatca cctggccaag gcgatagaga tttctttaga taatttacag
     9301 acagaaaagt acattaagag cctactcatt acattcttag gaatgaatat gagtaggctc
     9361 taatttattt agctatgcaa ttatatttat tgatagttaa ccatgaagta ttttttctta
     9421 ccgcgacgaa taatcgtaaa ttcgccatca attttatctt ctggtgctaa agcataatta
     9481 acatcttgtt gtctctcacc attaatataa atcgcaccat tgttaacatc ttcacgtgct
     9541 tgtcgtttag aaggagaaat gcctgtttca ataaggactt caacgatatt tgttgtgtca
     9601 tttgataatg tcacttgagg cacatcttta aatccatctt ttaattcttt cgctgataat
     9661 gattttaaat caccactaaa taatgcttgt gaaatacgga ttgcatcatt taatgcatct
     9721 tcaccatgaa taaatttagt tacttcttca gctaatgttt tttgagcttc acgtaaatgc
     9781 ggtgcttcat ttttagattg ttctaagcga tcaatttctt cttttcctaa gaaagtaaag
     9841 tattttaaga atttaattac atcttcgtct gattgattaa tccagaattg ataaaattca
     9901 taaggacttg ttttttcagc atctaaccaa acagcacctg actcagactt accaaatttc
     9961 ttaccatctg atttagttac aagcggaata gttaaaccgt atgcgtctgt ttgaccatac
    10021 atacgacgca ttaattcaat accacttgtg atattacccc attgatctga tccacctact
    10081 tgaatcttac aattcaattc tctattcaaa tgaccgaaat caatagcttg taaaatcgtg
    10141 tatgtgaatt ctgtatatga aataccatgt tctaaacgac tttggattga atctttacct
    10201 aacatgtaat taacgccgac gtgtttacca tagtcacgta aaaaactaat taatgagatt
    10261 tgtcctaacc agtctctatt attaacaagc actgcaccat ggtctgttcc aaattcaaaa
    10321 atattgtgca tttgcttact aataccttcg atatttttat ctacttgttc ttctgtttgt
    10381 agcacacgtt cttctgattt acctgatgga tcaccaatca tacctgtacc accgccaatt
    10441 aaaacgatag gacgatgtcc atgttcttga aaacgtctta atgttaagaa tggtagtaag
    10501 tgaccaatat gtaaactatc tgccgttgga tcggcaccgc agtataacgt cacttgttct
    10561 ttatttaata aatcttcaat accttgttca tcagtttgtt gataaataag acctctccat
    10621 tttaaatctt caattaatac attcgtcatt attatttcct cctatttata attatttatt
    10681 gaatacttgt taaaatactt taaagttttt tgaacgtaaa aaaaaccctt acaacaaata
    10741 tgtaagggcg cgattgcacg ttaccaccaa acttaaacat aatcataaga taatgttcac
    10801 tctattaatg atacgttcat taataaacgt aggacatgtt agttataaag gtgtattcat
    10861 attattaaaa acactagttc acagcgacca ctagctctct gatgatttca aataatatta
    10921 cttgtccttt tatcctattc ttta
//
LOCUS       DAKTIP010000082        10878 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAKTIP010000082 DAKTIP010000000
VERSION     DAKTIP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.082"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            111..542
                     /gene="mraZ"
                     /locus_tag="O0305_001535"
     CDS             111..542
                     /gene="mraZ"
                     /locus_tag="O0305_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCY0274682.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            558..1493
                     /gene="rsmH"
                     /locus_tag="O0305_001536"
     CDS             558..1493
                     /gene="rsmH"
                     /locus_tag="O0305_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCY0274683.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            1507..1908
                     /gene="ftsL"
                     /locus_tag="O0305_001537"
     CDS             1507..1908
                     /gene="ftsL"
                     /locus_tag="O0305_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCY0274684.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            1889..4123
                     /locus_tag="O0305_001538"
     CDS             1889..4123
                     /locus_tag="O0305_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0274685.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVINMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            4415..5380
                     /locus_tag="O0305_001539"
     CDS             4415..5380
                     /locus_tag="O0305_001539"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCY0274686.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            5382..6731
                     /locus_tag="O0305_001540"
     CDS             5382..6731
                     /locus_tag="O0305_001540"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCY0274687.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            6747..8066
                     /locus_tag="O0305_001541"
     CDS             6747..8066
                     /locus_tag="O0305_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCY0274688.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQADFDEEIYLINKDFKKEESNDKNNDSASSHANDNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNVISVDNEQPQSAPKEQNSDSIDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEENPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8172..9584
                     /gene="ftsA"
                     /locus_tag="O0305_001542"
     CDS             8172..9584
                     /gene="ftsA"
                     /locus_tag="O0305_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCY0274689.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            9617..10789
                     /gene="ftsZ"
                     /locus_tag="O0305_001543"
     CDS             9617..10789
                     /gene="ftsZ"
                     /locus_tag="O0305_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCY0274690.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
BASE COUNT         4039 a         1475 c         2144 g         3220 t
ORIGIN      
        1 ataaatcggg catttttttg ttttttaaaa taaatttcac aaattttgta taaatagtgg
       61 tggatagtgg ggagatgtgg taaattatat ataaggtgag gtgataaaaa atgttcatgg
      121 gagaatacga tcatcaatta gatacaaaag gacgtatgat tataccgtcc aagtttcgtt
      181 atgacttaaa tgagcgtttt attatcacaa gaggccttga taaatgttta ttcggttaca
      241 ctctagacga atggcaacag attgaagaga aaatgaaaac cttacctatg acaaaaaaag
      301 acgcacgtaa gtttatgcgt atgttcttct ctggtgctgt tgaagtagaa cttgataagc
      361 aagggcgtat taacatccct caaaacttga ggaaatacgc taatttaact aaagaatgta
      421 cagtaatcgg tgtttcaaat cgtattgaga tttgggatag agaaacttgg aatgatttct
      481 atgaagaatc tgaagaaagt ttcgaagata ttgctgaaga tttaatagat tttgattttt
      541 aaaatggagg aattgaagtg tttcatcata tcagcgttat gttaaacgaa accattgatt
      601 atttaaatgt aaaagaaaat ggtgtgtaca ttgactgtac gctaggtgga gcgggacatg
      661 ccctttattt actaaatcaa ttaaatgacg acggaagatt aatagcaatc gatcaagacc
      721 aaactgcaat tgataatgct aaagaggtat taaaggatca tttgcataag gtgacttttg
      781 ttcatagcaa cttccgtgaa ttaactcaaa tattaaaaga cttaaacatt gaaaaagtag
      841 atggaattta ttacgacttg ggtgtttcaa gcccacaact cgacattcca gaacgaggat
      901 tcagttatca ccatgacgca acattagaca tgcgtatgga ccaaacacaa gaactaacag
      961 catatgaaat tgttaacaat tggtcatatg aagcgttagt gaagattttt tatcgctatg
     1021 gcgaggagaa attttcaaaa cagatagctc gaagaatcga agcacatcgc gaacaacaac
     1081 caataacaac aacattagaa ttagttgaca ttataaaaga aggtattcct gcaaaagcaa
     1141 gaagaaaagg cggacatcct gcaaaacgag tatttcaagc actacgaatt gcagtaaacg
     1201 atgaattgtc agcttttgaa gattcaatag aacaagcgat tgaattagtg aaagtagatg
     1261 gcaggatttc ggtaatcact ttccattctt tagaagatcg tttatgtaaa caggtgttcc
     1321 aagaatatga aaaaggtcca gaggtaccaa gaggattacc agttatacca gaagcatata
     1381 cacctaagtt aaagcgtgtt aatcgtaaac cgattaccgc tacagaagaa gatttagatg
     1441 acaataacag agcacgaagc gcgaaattac gtgtagctga aatacttaaa taaggagcaa
     1501 tttataatgg ctgtagaaaa agtgtaccaa ccatatgacg aacaagttta taatagtata
     1561 ccgaagcaac aaccacaaac taagcccgaa aagaagactg tttcgagaaa agtggttgta
     1621 caattaacta aatttgaaaa agttttatac ataactttga ttactgtaat tgctatgtta
     1681 agtatttata tgctatcttt aaaaatggat gcgtatgata cgcgaggaaa gattgcagat
     1741 ttagattata aaatagataa acaatcaagt gaaaacagtg ctttacaatc tgaaatcaaa
     1801 aagaattctt cttatgaacg catatacgaa aaggctaaga aacaggggat gagccttgag
     1861 aacgataatg taaaggtagt gcgtagtaat ggcgaagcaa aaaattaaaa ttaaaaaaaa
     1921 taaaataggg gcagtcctac ttgttggttt attcggactg ctctttttta tattggtttt
     1981 aagaatttca tatatcatga ttactggaca ttctaatggt caagatttag tcatgaaggc
     2041 aaatgaaaag tatttagtta agaatgcaca acaaccagaa cgaggaaaga tatatgatcg
     2101 taatggtaaa gtgctagcag aagatgtaga aagatataaa cttgttgcag taatagataa
     2161 aaaggcgagt gccaattcta aaaaacctag gcatgtagtt gataaaaaag agactgcaaa
     2221 gaaattatct acagtcatta atatgaagcc agaggaaatt gaaaagagac ttagtcaaaa
     2281 gaaagctttc caaattgaat ttggacgcaa aggaacaaat ttaacgtatc aggacaaatt
     2341 gaaaatagag aaaatgaatt tgcctggtat ttctttattg cctgaaacag aacgctttta
     2401 tccaaatggc aattttgcat cacacttaat tggtagagct cagaaaaatc cggatactgg
     2461 tgaacttaaa ggtgcacttg gagttgaaaa gatttttgat agttatttaa gtggatctaa
     2521 aggatcattg agatatattc atgatatttg gggatatatc gcaccaaata ctaaaaaaga
     2581 gaagcagcct aaacgtggtg atgatgtcca tttaacaatc gattcaaata ttcaagtatt
     2641 tgttgaagaa gctttagatg gcatggttga aagataccag ccgaaagatt tatttgcggt
     2701 tgtcatggat gccaaaactg gagaaatttt agcatacagt cagcgaccaa catttaatcc
     2761 tgaaactggt aaagactttg gtaaaaagtg ggcaaatgac ctttatcaaa acacatacga
     2821 gcctggatca acatttaaat catatgggtt agcagctgct attcaagaag gtgcttttga
     2881 tcctgataag aaatataaat ctggacatag agatattatg ggttcacgta tttcagactg
     2941 gaatagagtc ggttggggtg aaatcccaat gtcactcgga tttacttatt catctaatac
     3001 attgatgatg catttacaag atttagttgg tgcagacaaa atgaaatctt ggtatgaacg
     3061 atttggattt ggaaaatcaa ctaaaggtat gtttgatgga gaagcacctg gtcaaattgg
     3121 atggagtaat gagttgcaac aaaaaacgtc atcatttggt caatcgacaa cagtaacacc
     3181 tgttcaaatg ttacaagcgc aatcagcgtt ctttaatgat ggtaatatgt taaaaccatg
     3241 gtttgtgaat agcgttgaaa atcctgttag taaaagacaa ttttataaag ggcaaaaaca
     3301 aatcgcaggc aaaccaataa caaaagatac tgctgaaaaa gttgaaaagc aattggattt
     3361 agttgtgaat agtaagaaga gtcacgctgc aaactatcgt attgatggtt atgaggtcga
     3421 aggtaagact ggtacagcac aagtcgctgc acctaatggt ggtggatacg ttaaaggtcc
     3481 aaacccatat tttgtaagtt ttatgggtga cgcgccgaag aaaaatccta aagttattgt
     3541 atacgctggt atgagcttgg cacaaaaaaa tgaccaagaa gcttatgaat taggtgttag
     3601 taaagcgttt aaaccaataa tggaaaatac tttgaaatat ttaaatgtag gtaaatcaaa
     3661 agatgacaca tctaatgcag agtatagtaa agtgccagat gttgaaggtc aagacaaaca
     3721 aaaagctatt gataatgtga gtgcaaaatc attagaacca gttactattg gttctggcac
     3781 acaaataaaa gcacaatcta taaaagcagg gaataaagtc ttacctcata gtaaagtact
     3841 gttattaaca gatggagact taactatgcc tgacatgtca ggatggacga aagaagatgt
     3901 cattgctttt gaaaacctaa caaatattaa agtaaattta aaaggtagcg gttttgtgtc
     3961 ccaccaatca attagtaagg gacaaaaact tactgaaaaa gataaaatag acgtagaatt
     4021 ttcatcagag aatgtagaca gcaattcgac gaataattct gattcaaatt cagatgataa
     4081 gaagaaatct gacagtaaaa ctgacaagga taagtcggac taacaagaat ggctacatta
     4141 aattaggatg tattgtcact atttgttttt actaaagtaa tgctgtgata gaaaacaaca
     4201 tcttcattta atctttataa ataagtgttg atagagaatt ttaaggtaga cgaggtcaga
     4261 ttaacttgtt tgttataggc aaacttttta tactggcctc ttatgccgta aaaaagaaaa
     4321 attataaatt ttgattacaa ctttgtttat ttaattgaaa aattgataat atattagtgg
     4381 ttaaaaaagt ctggattaag aagaggagat agttatgatt tttgtatatg cgttattagc
     4441 gctagtgatt acatttgttt tggtacctgt tttaatacct acattaaaaa ggatgaaatt
     4501 tggtcaaagt attcgagaag aaggtccaca aagccatatg aagaagactg gtacaccaac
     4561 gatgggtgga ctaacatttc tattaagtat tgtgataacg tctttggtgg ctattatatt
     4621 tgtagatcaa gctaatccaa tcatactgtt attatttgtg acgattggtt ttgggttaat
     4681 tggttttata gatgattata ttattgttgt taaaaagaat aaccaaggtt taacaagtaa
     4741 acagaagttt ttggcgcaaa ttggtattgc gattatattc tttgttttaa gtaatgtgtt
     4801 tcatttggtg aatttttcta cgagcataca tattccattt acgaatgtag caatcccact
     4861 atcatttgca tatgttattt tcattgtttt ttggcaagta ggtttttcta atgcagtaaa
     4921 tttaacagat ggtttagatg gattagcaac tggactgtca attatcggat ttacaatgta
     4981 tgccatcatg agctttgtgt taggagaaac ggcaattggt attttctgta tcattatgtt
     5041 gtttgcactt ttaggatttt taccatataa cattaaccct gctaaagtgt ttatgggaga
     5101 tacaggtagc ttagctttag gtggtatatt tgctaccatt tcaatcatgc ttaatcagga
     5161 attatcatta atttttatag gtttagtatt cgtaattgaa acattatctg ttatgttaca
     5221 agtcgctagc tttaaattga ctggaaagcg tatatttaaa atgagtccga ttcatcatca
     5281 ttttgaattg ataggatgga gcgaatggaa agtagttaca gtattttggg ctgttggtct
     5341 gatttcaggt ttaatcggtt tatggattgg agtgcattaa gatgcttaat tatacagggt
     5401 tagaaaataa aaatgtatta gttgtcggtt tggcaaaaag tggttatgaa gcagctaaat
     5461 tattaagtaa attaggtgcg aatgtaactg tcaatgatgg aaaagactta tcacaagatg
     5521 ctcatgcaaa agatttagaa tctatgggca tttctgttgt aagtggaagt catccattaa
     5581 cgttgcttga taataatcca ataattgtta aaaatcctgg aataccttat acagtatcta
     5641 ttattgatga agcagtgaaa cgaggtttga aaattttaac agaagttgag ttaagttatc
     5701 taatctctga agcaccaatc atagctgtaa cgggtacaaa tggtaaaacg acagttactt
     5761 ctctaattgg agatatgttt aaaaaaagtc gcttaactgg aagattatcc ggcaatattg
     5821 gttatgttgc atctaaagta gcacaagaag taaagcctac agattattta gttacagagt
     5881 tgtcgtcatt ccagttactt ggaatcgaaa agtataaacc acacattgct ataattacta
     5941 acatttattc ggcgcatcta gattaccatg aaaatttaga aaactatcaa aatgctaaaa
     6001 agcaaatata taaaaatcaa acggaagagg attatttgat ttgtaattat catcaaagac
     6061 aagtgataga gtcggaagaa ttaaaagcta agacattgta tttctcaact caacaagaag
     6121 ttgatggtat ttatattaaa gatggtttta tcgtttataa aggtgttcgt attattaaca
     6181 ctgaagatct agtattgcct ggtgaacata atttagaaaa tatattagca gctgtgcttg
     6241 cttgtatttt agctggtgta cctattaaag caattattga tagtttaact acattttcag
     6301 gaatagagca tagattgcaa tatgttggta ctaatagaac taataaatat tataatgatt
     6361 ccaaagcaac aaacacgcta gcaacacagt ttgccttaaa ttcatttaat caaccaatca
     6421 tttggttatg tggtggtttg gatcgaggga atgaatttga cgaactcatt ccttatatgg
     6481 aaaatgttcg cgcgatggtt gtattcggac aaacgaaagc taagtttgct aaactaggta
     6541 atagtcaagg gaaatcggtc attgaagcga acaatgtcga agacgctgtt gataaagtac
     6601 aagatattat agaaccaaat gatgttgtat tattgtcacc tgcttgtgcg agttgggatc
     6661 aatatagtac ttttgaagag cgtggagaga aatttattga aagattccgt gcccatttac
     6721 catcttatta aagggtgtga gtattgatgg atgataaaac gaagaacgat caacaagaat
     6781 caaatgaaga taaagatgaa ttagaattat ttacgaggaa tacatctaag aaaagacggc
     6841 aaagaaaaag atcaaaggct acacattttt ctaatcaaaa taaagatgat acatctcaac
     6901 aagctgattt tgatgaagaa atttacttga taaataaaga cttcaaaaaa gaagaaagca
     6961 atgataaaaa taatgattct gcttctagtc atgcgaatga taataatatc gatgattcta
     7021 cagactctaa tattgaaaat gaggattata gatataatca agaaattgac gaccaaaatg
     7081 aatcgaatgt aatttcagtc gacaacgaac aacctcaatc agctcctaaa gaacaaaata
     7141 gcgactcgat tgatgaggaa acagtaacga aaaaagaacg aaaaagtaaa gtaacacaat
     7201 taaagccatt aacacttgaa gaaaagcgga agttaagacg taagcgacaa aagcgaatcc
     7261 aatacagtgt tattacaata ttggtattgt tgattgctgt tatattaatt tacatgtttt
     7321 caccacttag taaaattgcg catgtaaata taaatggaaa taatcacgtt agtacttcaa
     7381 agataaacaa agttttaggt gttaaaaatg attcaaggat gtatacgttt agtaaaaaaa
     7441 atgctattaa tgatctcgaa gagaatccat taatcaaaag tgttgagata cacaagcaat
     7501 taccaaacac attaaacgta gatatcacag aaaatgaaat tattgcttta gtgaaatata
     7561 aaggtaaata tttaccttta ttagaaaatg gtaaattgct taaaggttca aatgatgtca
     7621 aaattaatga tgcacctgtc atggatggtt tcaaaggtac aaaagaagat gatatgatta
     7681 aggcgttatc tgaaatgaca cctgaagtta gacgatatat tgccgaagtg acatacgccc
     7741 caagtaaaaa caaacaaagc agaattgaat tgtttacgac agatggactt caagtaatcg
     7801 gtgatatttc gacgatatct aagaaaatga aatattatcc gcagatgtca caatcattat
     7861 caagggatag ttcgggtaaa ctaaaaacac gaggctatat tgatttatca gtcggtgctt
     7921 catttatccc ataccgtgga aacacgtcta gtcaatcaga aagcgataaa aatgtgacta
     7981 aatcatctca agaggaaaat caagcaaaag aagaattaca aagcgtttta aacaaaatta
     8041 acaaacaatc aagtaagaat aattaaaaaa ttttgatatt gtctatgttt atagttcaca
     8101 agccattcaa cgtattgtaa actaaggata gtgtattttt ttaatagtaa tttgtcagga
     8161 ggtgcctatc tatggaagaa cattactacg taagtattga tattggatca tcaagcgtaa
     8221 aaacaatagt aggcgagaaa tttcacaatg gtataaatgt gataggtaca ggacaaacct
     8281 acacgagcgg tataaaaaat ggtttaattg atgattttga tattgcgcga caagcaatca
     8341 aagacacaat taaaaaggca tcaatcgctt cgggtgttga tattaaagaa gttttcctga
     8401 aattacctat cattggaacg gaagtttatg atgaatcaaa tgaaatcgac ttttatgagg
     8461 atacagaaat caacggttca catatcgaaa aagtattaga aggtattaga gaaaaaaatg
     8521 atgtgcaaga aacagaagta attaatgtgt tcccgattcg ttttatagtc gataaagaaa
     8581 atgaggtttc agaccctaaa gaattaattg ccagacattc attaaaggtt gaagcaggcg
     8641 taattgctat tcaaaaatcg attttaatta atatgattaa atgcgtagaa gcatgtggtg
     8701 ttgatgtatt agatgtttac tctgatgcat ataactatgg ttcaatccta acagctactg
     8761 aaaaagagtt aggtgcatgt gtcattgata ttggtgaaga cgttacgcaa gttgcttttt
     8821 atgaacgcgg tgaattagta gatgctgatt ctatcgaaat ggcagggcgt gatattacag
     8881 acgatattgc acaaggatta aacacttctt atgaaactgc tgaaaaagtt aaacaccaat
     8941 atggtcatgc attctatgat tctgcttcag atcaagatat cttcactgtt gaacaggttg
     9001 atagtgatga aacagtacag tatactcaaa aagatttgag tgactttatt gaagcgcgtg
     9061 tagaagaaat attcttcgaa gtatttgatg ttttacaaga tttaggatta acaaaagtaa
     9121 atggtgggtt tattgtaact ggtggatctg caaacttact tggcgtaaaa gaattattat
     9181 cagatatggt aagtgaaaaa gttagaattc acacgccatc acaaatggga attagaaaac
     9241 ctgaattttc ttcagcaatt tctacaattt ctagtagtat cgcttttgat gagttattag
     9301 attatgttac aattaattat catgataatg aagaaactga agaagatgtt attgatgtga
     9361 aagacaaaga taacgaatct aaattaggcg gatttgattg gtttaaacgt aaaacaaaca
     9421 aaaaagatac tcatgaaaat gaagtagagt caacagatga agaaatttat caatcagaag
     9481 ataatcatca ggaacataaa cagaatcatg aacatgttca agacaaagat aaagataaag
     9541 aagaaagtaa attcaaaaaa ctaatgaaat ctctatttga atgattattg gccaataaaa
     9601 ctaggaggaa atttaaatgt tagaatttga acaaggattt aatcatttag cgactttaaa
     9661 ggtcattggt gtaggtggtg gcggtaacaa cgccgtaaac cgaatgattg accacggaat
     9721 gaataatgtt gaatttatcg ctatcaacac agacggtcaa gctttaaact tatctaaagc
     9781 tgaatctaaa atccaaatcg gtgaaaaatt aacacgtggt ttaggagcag gagctaatcc
     9841 tgaaatcggt aaaaaagctg cagaggaatc tcgtgaacaa attgaagatg caatccaagg
     9901 tgcagacatg gtatttgtta cttctggtat gggtggcgga actggtactg gtgcagcacc
     9961 agtcgttgct aaaattgcaa aagaaatggg cgcattaact gttggtgttg taactcgtcc
    10021 atttagtttt gaaggacgta aacgtcaaac tcaagctgct gctggagtag aagctatgaa
    10081 agctgcagta gatacattaa tcgttatacc aaatgaccgt ttattagata tcgttgacaa
    10141 atctacgcca atgatggaag catttaaaga agctgacaac gtgttacgcc aaggtgtaca
    10201 aggtatctca gacttaatcg ctgtttctgg tgaagtaaac ttagactttg cagacgttaa
    10261 gacaattatg tctaaccaag gttctgcatt aatgggtatt ggtgtttctt ctggtgaaaa
    10321 tagagcggta gaagctgcta aaaaagcaat ctcttctcca ttacttgaaa catctatcgt
    10381 tggtgcacaa ggtgtgctta tgaatattac tggtggcgag tcattgtcat tatttgaagc
    10441 acaagaggct gctgatattg tccaagatgc tgcagatgaa gacgttaata tgattttcgg
    10501 tacagttatt aatcctgaat tacaagatga gattgttgta acagttattg caactggttt
    10561 tgatgacaaa ccaacatcac atggtcgtaa atctggtagc actggattcg gaacaagcgt
    10621 aaatacttct agcaatgcaa cttctaaaga tgaatcattc acttcaaatt catcaaatgc
    10681 acaagcaact gatagtgtaa gtgaaagaac acatacaact aaagaagatg atattcctag
    10741 cttcattaga aatagagaag aaagacgttc aagaagaaca agacgttaat cggttaatat
    10801 atatacacaa ataattcaac acaaatcatc agataacata tctgatgatt tttttactaa
    10861 tttttagaac atgtagaa
//
LOCUS       DAKTIP010000083        10848 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAKTIP010000083 DAKTIP010000000
VERSION     DAKTIP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.083"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            180..455
                     /gene="rpsP"
                     /locus_tag="O0305_001544"
     CDS             180..455
                     /gene="rpsP"
                     /locus_tag="O0305_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCY0274691.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            643..1146
                     /gene="rimM"
                     /locus_tag="O0305_001545"
     CDS             643..1146
                     /gene="rimM"
                     /locus_tag="O0305_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCY0274692.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            1146..1883
                     /gene="trmD"
                     /locus_tag="O0305_001546"
     CDS             1146..1883
                     /gene="trmD"
                     /locus_tag="O0305_001546"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCY0274693.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            1986..2336
                     /gene="rplS"
                     /locus_tag="O0305_001547"
     CDS             1986..2336
                     /gene="rplS"
                     /locus_tag="O0305_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCY0274694.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(2580..5186)
                     /gene="yfhO"
                     /locus_tag="O0305_001548"
     CDS             complement(2580..5186)
                     /gene="yfhO"
                     /locus_tag="O0305_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCY0274695.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLRSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSANMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVCI
                     IAILYVLLSPTHPLALIVGIILLIVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSLSTLKQHDYHSNYVNQLIKKINQNATGSFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKIKSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNIKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRVVTPVTIRIKAPDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKHGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            5587..6471
                     /gene="ylqF"
                     /locus_tag="O0305_001549"
     CDS             5587..6471
                     /gene="ylqF"
                     /locus_tag="O0305_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCY0274696.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            6455..7222
                     /locus_tag="O0305_001550"
     CDS             6455..7222
                     /locus_tag="O0305_001550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCY0274697.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            7331..8497
                     /gene="sucC"
                     /locus_tag="O0305_001551"
     CDS             7331..8497
                     /gene="sucC"
                     /locus_tag="O0305_001551"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCY0274698.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            8519..9427
                     /gene="sucD"
                     /locus_tag="O0305_001552"
     CDS             8519..9427
                     /gene="sucD"
                     /locus_tag="O0305_001552"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCY0274699.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            9654..10772
                     /locus_tag="O0305_001553"
     CDS             9654..10772
                     /locus_tag="O0305_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0274700.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGAVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVINNNELTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     KTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKAEVAHKVVHNYENDMIFIRPFKKA"
BASE COUNT         3992 a         1489 c         2023 g         3344 t
ORIGIN      
        1 tatgaatttt tataacctat taacagtcta atttagtaat cacatagtta ttattgtaag
       61 aaaaaggttc aataattttc ctggtaaaga aaaaactctt tacaaacatt catacacctg
      121 ttaatattat ttcttgtaga aaataaaaat taaaacatga cttaaaggag attttataaa
      181 tggcagttaa aattcgttta acacgtttag gttcaaaaag aaatccattc tatcgtatcg
      241 tagtagcaga tgctcgttct ccacgtgacg gacgtatcat cgaacaaatc ggtacttata
      301 acccaacgag cgctaatgct ccagaaatta aagttgacga agcgttagct ttaaaatggt
      361 taaatgatgg tgcgaaacca actgatacag ttcacaatat cttatcaaaa gaaggtatta
      421 tgaaaaaatt tgacgaacaa aagaaagcta agtaatttag cgtaaaattg ttctaacaat
      481 aagaataact cgtttacact gacagttatt actcaatgat acgttgggaa tatcacatgt
      541 tagtaatata gaacgtattg ggtaccataa tggtgccctt tttctttgaa ttattttcaa
      601 ttaaaataga agtgttcaaa gcatagagtt ggaggtaata gaatgagagt tgaagttggt
      661 caaattgtta acacacatgg tattaaaggt gaaattaaag taaaatccaa ttcagacttt
      721 acagacgttc gttttcaacc cggtcaagtg ctgacagttg tgcataacaa taacgacctt
      781 gaatatactg ttaagtcaca tagagtgcat aaagggcttc atatgcttac atttgaaggt
      841 attaataata ttaatgatat tgagcattta aaagggagtt ctatttatca agagcgtgat
      901 catgaagata tcgtacttga ggaaaatgaa ttttattatt cagatattat aggatgtaca
      961 gtttttgatg atcaagaaac accaataggt cgtgtaatta atatatttga aacaggtgcg
     1021 aatgatgtgt gggtgattaa aggatctaaa gaatatttga ttccttatat tgctgatgtt
     1081 gtaaaagaag tggatgttga aaataaaaaa attatcatca cgccaatgga aggattgttg
     1141 gattaatgaa aattgattat ttaactttat ttcctgaaat gtttgatggt gttttaaatc
     1201 attcaattat gaaacgtgcc caagaaaaca ataaattaca aatcaatacg gttaatttta
     1261 gagattatgc aattaacaag cacaaccaag tagatgatta tccgtatggt ggcggacaag
     1321 gtatggtgtt aaagcctgaa cctgttttta atgcgatgga agacttagat gtcacagaac
     1381 aaacacgcgt tattttaatg tgtccacaag gcgagccatt ttcacatcag aaagctgttg
     1441 aattaagcaa ggccgaccac atcgttttca tatgcggaca ttatgaaggt tacgatgaac
     1501 gtatccgaac acatcttgtc acagatgaaa tatcaatggg tgactatgtt ttaactggtg
     1561 gagaattgcc agcgatgacc atgactgatg ctattgttag actgattcca ggtgttttag
     1621 gtaatgaaca gtcacatcaa gacgattcat tttcagatgg gttattagag tttccgcaat
     1681 atacacgtcc gcgtgaattt aagggtctaa cagttccaga tgttttattg tctggaaatc
     1741 atgccaatat tgatgcatgg agacatgagc aaaagttgat ccgcacatat aataaaagac
     1801 ctgacttaat tgaaaaatat ccattaacta atgcagataa gcaaatatta gaaagatata
     1861 aaataggatt gaaaaaaggt tagtattgtg ctaacataat ctgagtgtgg aaacactaaa
     1921 atcactatga tccgctgcta tatatttgtc gaggcaagaa cataggttga agaggagaat
     1981 ttataatgac aaatcacaaa ttaatcgaag cagtaactaa atcacaatta cgtacagact
     2041 taccaagttt ccgtcctggt gatactttac gtgtacacgt acgtatcatt gagggtactc
     2101 gtgagcgtat ccaagtattc gaaggcgttg taattaaacg tcgtggcggt ggcgtttctg
     2161 aaacgtttac agttcgtaaa atttcatcag gtgttggcgt ggaacgtaca ttcccattac
     2221 acacaccaaa aattgaaaaa atcgaagtta aacgtcgtgg taaagtacgt cgtgctaaat
     2281 tatattactt acgtagttta cgtggtaaag ctgctagaat ccaagaaatt cgttaatcag
     2341 catttaaaca aagctatgta tgagtcaaat tcgactcaaa caataaagcc atctaggtca
     2401 cttttatgga gtgatataga tggctttttt tgctgtttag ttaatgtaaa gttgataatg
     2461 taattgttta ttccttctct attcataacg tgtttgctca aaatggttat ataaacaaaa
     2521 atctactaag tgccattgaa aaggacttag tagatttatt aaatatatgc gtagcaacat
     2581 tatttttgtc ttgcccatct cgtgaaaata atactacata taatgccaaa tatagtaatt
     2641 gttataagta aataatagta tggtggggta tagctcaatt gaatctttgt tatattttta
     2701 ggtgctttaa tgccggtcat aacaccattt acttgttcaa ctttaagact ttgatcacct
     2761 gatgtcgctt tcataccttg attatatgct gttggcaaaa caatataccc agatgaattt
     2821 ttatttttag taatagtata accatgcttt gtcttactaa ctttgacagc ttctaatgaa
     2881 tttgaagcgt ctttaagcgt ggtataatct tcgccgtata tcccttttaa atttactcga
     2941 tacttacctt taggcaatga taatctaatt ctatctggag ctttaatgcg tatcgttacg
     3001 ggtgttacaa cgcgtcgata tttataagtg agtttatttc tttcttgtgt atattcattc
     3061 actttaacat catgagcttt atccggcgaa agtaattcta aatccatttc aaaatacaaa
     3121 tctttaaatt gattagaaac tgattttggc aactgtacag ttagaccacc attattttgt
     3181 ttaacttgta ataaatgttt tgtaggagat tgccaggctg cactatttaa tttaattgtt
     3241 gaatctgata gtaaattttt attggcttta aaatgtgtat taacatcttt aatattgtta
     3301 gaaacaatcc cttgcaacat tgcttgttct ttatctaatg gagattttaa ttctttattg
     3361 gaaaagacct tatttgtaat atgtgcactt ggataatgga tggtattttt agaatgaatc
     3421 caacgaactt tattgtcttt gtgttcagac ttaattttaa atccatatgg taagttgtca
     3481 tcatgattca ctctaattcg atcattaaca ttccaaagtg atagtaaatt ttgacgattg
     3541 ccaagtaatc tataagtgct gtttttatcg attggcatat taatttggag tgtcttgtca
     3601 taatatttta aaatgtctcc attaaaaata ctagaatata atgaaatgcc attataatga
     3661 tatataaatg gtgaatttaa tgcatagtct gacatataat caatgcgatt aaatgagcct
     3721 gttgcatttt gatttatctt ttttataagc tggtttacat agttactatg gtaatcatgt
     3781 tgtttcaacg ttgataatga ttgttgataa ggtttgattg ccatgttttt gttgttatct
     3841 aaaatgacga tttgttgaat cataacgatt aatactaata ttgcaacggt taatttttta
     3901 taacgccata aactaaattt taaaataacg gcaagcacta ttagcaggat aatacctact
     3961 ataagtgcaa gtgggtgtgt cggtgatagt aatacataaa gtattgcgat gatgcatacg
     4021 ggtattgttc tgattaaata atatttcata tttaatgttg ataaatgttg aataaacaat
     4081 ccgcaaagag cacttgatga tagtgctaag atatacaccc aacgcctttc tggaaatgaa
     4141 aaaccattaa aagcactgtc gaaatactgt gataatgaac ctataaataa tatccatgtt
     4201 actattgcga aaagtctata aaagtaaaaa cgatacagtt tgaatgacaa taatgcaacg
     4261 atagtaagaa ttgaaatcgt aatataaaat ccatcgctaa agaaaaaata atgataatca
     4321 agtggtgtca aaaacggtat atcaacattg ggattttgct ttctgtcatt ttccaaaaac
     4381 gcagaaatgc cagtgaataa accaaataca cttgataaca cactcaaaac tgtagcagat
     4441 atgacgcaaa ttaatttttg tgttctagag acaatgtcat atttgtaagt gaaaatgagt
     4501 cgatataaat agtagcaacc tataataata gcttgataat aactgaaata aaaattgcta
     4561 aatagtgtta aggctatcgc aacaatgaaa ataccgattt tgcgttgttg aaaatatctt
     4621 tccaaaccaa gaatcgataa tggcaataaa tataataaat ttccataaaa tgaccaagta
     4681 aaattaaagt atataacgac agttgacatg ccgtataaaa tcgtagcgat catatttgct
     4741 gagcgtttaa agtgtaatat tttaaataag tagaaggtca cgacaaatgt tatgatagct
     4801 cgtatcatag ccataataag ttggtttgtc ggccaaaaat gtattgtcgt cggattaaat
     4861 ataccaaccg tttctcctat tttaatgaat agaaaattta gccacattaa aggtgacagc
     4921 gaataataat atgatagtcc tttcatataa tcgccaccta atccaaacga tgcatcatat
     4981 aaactagaaa aactacgtag atgttcatac aaatacattt gaaatggcat catttgacgg
     5041 aatccatctc cagccccgct aaaaacagta ccattcacaa tataatcata gatatgagta
     5101 gaaaataaaa taagcgttaa tattacacta atgaaagtta taacaaagaa ttgtttgacg
     5161 tttgaattta gccacttttt taacacaaca ttatcctcaa ctttcaaatt taaaattaag
     5221 tttaactgaa actaaagtta atgaggttct tgataggtaa agacgaagat gactgtggaa
     5281 cagatacctt atcatagtta cttaaacttt ggatcatttt cagtttatca ttaaacaaat
     5341 atattgaata ataaaaatgt catactgata aagatgaatg tcacttaata agtaacttag
     5401 atttaacaaa tgatgatttt taattgtaga aaacttgaaa taatcactta tacctaaatc
     5461 taaagcattg ttaagaagtg tgacaatgtt aaaataaata tagttgaatt aatgaatttg
     5521 ttctataatt aacaggttat agattttaat aatgagaaaa gaattgacga aagtaaggtg
     5581 aattgaatgg ttattcaatg gtatccagga catatggcga aagccaaaag agaagtaagt
     5641 gaacaattaa aaaaagtaga tgtagtgttt gaactagtag atgcaagaat tccatatagt
     5701 tcaagaaacc ctatgataga tgaagttatt aaccaaaaac cacgtgttgt tatattaaat
     5761 aaaaaagata tgtctaattt aaatgagatg tcaaaatggg aacaattttt tattgataaa
     5821 ggatactatc ctgtatcagt ggatgctaag cacggtaaaa atttaaagaa agtggaagct
     5881 gcagcaatta aggcgactgc tgaaaaattt gaacgcgaaa aagcgaaagg acttaaacct
     5941 agagcgataa gagcaatgat cgttggaatt ccaaatgttg gtaaatccac attaataaat
     6001 aaactggcaa agcgtagtat tgcgcagact ggtaataaac caggtgtgac caaacaacaa
     6061 caatggatta aagttggtaa tgcattacaa ctattagaca caccagggat actttggcct
     6121 aaatttgaag atgaagaagt cggtaagaag ttgagtttaa ctggtgcgat aaaagatagt
     6181 attgtgcact tagatgaagt tgccatctat ggattaaact ttttaattca aaatgattta
     6241 gcgcgattaa agtcacatta taatattgaa gttcctgaag atgcagaaat catagcgtgg
     6301 tttgatgcga tagggaaaaa acgtggctta attcgacgtg gtaatgaaat tgattacgaa
     6361 gcagtcattg aactgattat ttatgatatt cgaaatgcta aaataggaaa ttattgtttt
     6421 gatattttta aagatatgac tgaggaatta gcaaatgacg ctaacaatta aagaagttac
     6481 gcagttgatt aatgcggtta atacaataga agaattagaa aatcatgaat gctttttaga
     6541 tgagcgaaaa ggtgttcaaa atgccatagc taggcgcaga aaagcgttag aaaaagaaca
     6601 agctttaaaa gaaaagtatg ttgaaatgac ttactttgaa aatgaaatat taaaagagca
     6661 tcctaatgct attatttgtg ggattgatga agttggaaga ggacctttag caggtccagt
     6721 cgttgcatgc gcaacaattt taaattcaaa tcacaattat ttgggccttg atgactcgaa
     6781 aaaagtacct gttacgaaac gtctagaatt aaatgaagca ctaaaaaatg aagttactgc
     6841 ttttgcatat gggatcgcga cagctgaaga aatagatgaa tttaatattt ataaagctac
     6901 tcaaatcgcc atgcagcgag ctattgatgg attatcagta caaccaacgc atttattgat
     6961 agacgcgatg acgcttgata atgcactgcc tcaagtatct ttaatcaagg gtgatgcaag
     7021 aagtgtgtcc attgcagctg caagtatcat ggcaaaggtt tttcgtgatg attatatgac
     7081 acagttatct aaagattatc ctgaatatgg ttttgaaaaa aacgcgggtt acggtaccaa
     7141 acaacattta ctagcaatcg atgatattgg cattatgaaa gagcatagaa aaagctttga
     7201 acctataaaa tcgttactgt aaatcgtaaa attcaattaa atgataaaaa agtagcattt
     7261 aagtttacaa tagcgcttac attttttata attgtaaggg aacttaacct aagtaacagg
     7321 aggatggaag atgaatatcc acgagtatca aggtaaagaa atatttcgtt caatgggcgt
     7381 tgcagttcca gaaggacgag tagcatttac tgctgaagaa gcggtggaga aagcaaaaga
     7441 attaaattct gatgtttatg ttgtaaaagc acaaattcat gctggaggta gaggtaaagc
     7501 aggcggagta aaaattgcta aatctttatc tgaggtagaa acatatgcaa aagaattatt
     7561 agggaaaact ttggtgacac atcaaactgg tccagaaggt aaagaaatta agcgtttata
     7621 tatcgaagaa ggttgtgcta ttcaaaaaga atattacgtt ggattcgtta ttgatcgtgc
     7681 gactgaccaa gtaacattga tggcgtctga agaagggggc actgagattg aagaagttgc
     7741 tgcgaagact cctgaaaaga tcttcaaaga aactatcgat ccagtaatcg gactttcacc
     7801 attccaagca agacgaattg cgtttaatat taatattcct aaagaatctg ttaacaaagc
     7861 tgctaaattc ttattagcac tttataatgt attcattgaa aaagattgtt caatcgtaga
     7921 aatcaaccca ttagttacaa cagctgatgg tgatgtattg gcattagatg ctaaaattaa
     7981 ttttgatgat aatgcattat tcagacataa agatgttgta gaattacgtg atttagaaga
     8041 agaagatccg aaagagattg aagcgtctaa acatgattta tcatacattg cattagatgg
     8101 tgacatcgga tgtatggtta atggtgcagg tttagccatg gcaacaatgg atacgattaa
     8161 tcatttcggt ggaaacccag ccaatttctt agatgcaggc ggaagcgcta ctagagaaaa
     8221 agtaactgaa gcatttaaaa tcattttagg tgatgaaaat gttaaaggta tttttgtaaa
     8281 cattttcggt ggcattatga aatgtgatgt tatcgcagaa ggtatcgttg aagctgtaaa
     8341 agaagtagat ttaactttac cactagttgt acgcctagaa ggtacaaatg ttgagttagg
     8401 taaaaaaatc ttaaaagact caggattagc aattgaacca gcagcaacaa tggctgaagg
     8461 tgcacaaaaa attgttaaac tagtcaaaga agcataagga aaggatggga gcactaagat
     8521 gagtgtattt atagataaga atactaaagt aatggtacaa ggtattacag ggtctactgc
     8581 ccttttccat acaaaacaaa tgcttgatta tggtacgaaa atagtagcag gtgtgacgcc
     8641 tggtaaaggt ggtcaagttg ttgaaggcgt tcctgttttc aacactgttg aagaagctaa
     8701 aaatgaaact ggggcaacgg tttcagtcat ttacgttcca gcaccatttg ctgcagactc
     8761 aattttagaa gcagctgatg cagacttaga tatggttatt tgtatcactg aacatattcc
     8821 tgtattagac atggttaaag ttaaacgcta cttacaaggt agaaaaacac gtttagttgg
     8881 tccgaactgt ccaggtgtga ttacagcaga tgaatgtaaa attggtatta tgcctggcta
     8941 tattcacaaa aaaggtcatg ttggtgtagt atctcgttca ggtacattaa catatgaagc
     9001 agtgcaccaa ttgactgaag aaggtattgg tcaaactaca gctgttggta ttggtggaga
     9061 cccagtcaac ggaacaaact ttattgatgt tttaaaagca ttcaatgaag atgacgaaac
     9121 gaaagcagtt gttatgattg gtgaaatcgg tggtacggct gaagaagaag cagctgaatg
     9181 gattaaagcg aatatgacaa aaccagttgt aggctttatc ggtggacaaa cagcacctcc
     9241 tggaaaacgt atgggacatg ctggtgcaat catttcaggt ggtaaaggta ctgctgaaga
     9301 gaaaattaaa acattaaata gttgtggtgt gaaaacagcg gcaacacctt cagaaattgg
     9361 ttcaacatta attgaagctg ctaaagaagc aggtatttat gaatcattat taactgttaa
     9421 taaataaagt taaaagatga tataaatggc tatagcccat tccatacttt ataaaagtat
     9481 cggaaatggg ctatttgtcg tgcgatgaat tttagtacac ttttcatatt ttggaaagac
     9541 aagttaatta ttttacctcc tacaatagct ttataaaaca aagaaaattt taattaaaat
     9601 atatagcgca ctatgttata atgtttgtat attattgtaa ggagtgtttc atcatgaata
     9661 aacaacaaag taaagtacgc tattcaatta gaaaagttag tattggaatt ttgtcaattt
     9721 caataggtat gtttttggca ttgggtatgt cgaacaaagc atatgcagat gaaattgata
     9781 aatctaaaga ttttacaaga gggtatgagc aaaatgtatt cgcgaaatca gagttaaatg
     9841 ctaataaaaa tacgacaaaa gacaaaataa aaaatgaagg tgctgttaaa acatcggaca
     9901 caagtttaaa gttagacaac aaatcagcaa tttcaaacgg aaatgaaatt aatcaagata
     9961 taaagatttc aaatactccg aaaaactcaa gccaaggtaa caatctagtt attaataaca
    10021 atgaacttac taaagaaatt aaaattgcaa acttggaagc tcaaaattct aatcagaaga
    10081 aaacgaataa agttactaat aattactttg gttactacag ttttagagaa gctccaaaaa
    10141 cacaaatcta tactgtaaaa aaaggagaca cacttagtgc tatagcatta aaatacaaaa
    10201 ctacagtttc aaatattcaa aatacaaata atatagcaaa tcctaattta atatttattg
    10261 gtcaaaaatt aaaagtgcca atgacaccat tagtagaacc aaaaccaaaa acagtgtctt
    10321 caaataataa aagtaatagt aatagcagta cattaaatta tttgaaaaca ttagagaata
    10381 gaggatggga tttcgacggt agttatggat ggcaatgttt cgatttagtt aatgtatatt
    10441 ggaatcatct ttatggtcat ggattaaaag gatatggagc taaagatata ccatatgcaa
    10501 ataattttaa tagtgaagct aaaatttatc acaacacacc aactttcaaa gctgaacctg
    10561 gggacttagt ggtttttagt ggaagatttg gtggaggata tggtcataca gctattgtct
    10621 taaatggtga ttatgatgga aaattaatga agttccaaag tttagatcaa aactggaata
    10681 atggtggatg gcgtaaagca gaggttgcac ataaagttgt tcataattat gaaaatgata
    10741 tgatttttat tagaccattt aaaaaagcat aatttaaatc gaaggcagga cattgaaata
    10801 tgaaattttc aactttaagt gaagaagaat ttaccaacta caccaaaa
//
LOCUS       DAKTIP010000084        10821 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAKTIP010000084 DAKTIP010000000
VERSION     DAKTIP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.084"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            25..633
                     /locus_tag="O0305_001554"
     CDS             25..633
                     /locus_tag="O0305_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCY0274701.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            970..2178
                     /gene="gltS"
                     /locus_tag="O0305_001555"
     CDS             970..2178
                     /gene="gltS"
                     /locus_tag="O0305_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCY0274702.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVAALDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(2226..3275)
                     /locus_tag="O0305_001556"
     CDS             complement(2226..3275)
                     /locus_tag="O0305_001556"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCY0274703.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(3306..4253)
                     /gene="corA"
                     /locus_tag="O0305_001557"
     CDS             complement(3306..4253)
                     /gene="corA"
                     /locus_tag="O0305_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCY0274704.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            4350..4841
                     /locus_tag="O0305_001558"
     CDS             4350..4841
                     /locus_tag="O0305_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCY0274705.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQSTITWPEQLGHKIAIPHKG
                     ALK"
     gene            4838..5257
                     /locus_tag="O0305_001559"
     CDS             4838..5257
                     /locus_tag="O0305_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274706.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(5365..6285)
                     /locus_tag="O0305_001560"
     CDS             complement(5365..6285)
                     /locus_tag="O0305_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274707.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            6493..7131
                     /locus_tag="O0305_001561"
     CDS             6493..7131
                     /locus_tag="O0305_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274708.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(7377..9308)
                     /locus_tag="O0305_001562"
     CDS             complement(7377..9308)
                     /locus_tag="O0305_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCY0274709.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNK
                     DEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSPMLNLEVLKFP
                     TFTLTTIINMVVMLSLYGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGK
                     LLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTA
                     AINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVV
                     QDHMHELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSN
                     KKAKATAQKLDADNSINHE"
     gene            complement(9321..9968)
                     /locus_tag="O0305_001563"
     CDS             complement(9321..9968)
                     /locus_tag="O0305_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCY0274710.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVAIVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            10089..10640
                     /locus_tag="O0305_001564"
     CDS             10089..10640
                     /locus_tag="O0305_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCY0274711.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
BASE COUNT         3548 a         1873 c         1624 g         3776 t
ORIGIN      
        1 tcatataatt gcgaggagaa tattatggat ttcgttaata atgatacaag acaaattgct
       61 aaaaacttat taggtgtcaa agtgatttat caggatacca ctcaaacgta tacaggctac
      121 atcgtggaaa cggaagctta cttaggtttg aatgatcgtg cggctcatgg ctatggcggt
      181 aaaataacac ctaaagtcac gtcattatat aaacgtggtg gtacaattta tgcacatgtc
      241 atgcatacgc atttactcat taattttgta acaaaatctg aaggtatacc tgaaggcgta
      301 cttatccgcg caattgaacc agaagaaggt ttatccgcta tgttccgtaa cagaggtaag
      361 aaaggctacg aggtaacgaa tggcccagga aaatggacta aggcatttaa cattccacgg
      421 gctatcgatg gcgctacgtt aaatgactgt agattgtcta ttgatactaa gaatcgtaaa
      481 tatcctaaag atattattgc tagtccacga atcggtattc caaataaagg tgattggaca
      541 cataaatctt tacgttacac agtgaaaggt aatccatttg tgtctcgcat gcgtaaatca
      601 gattgtatgt ttcccgaaga tacttggaaa taaatgccat ctttcattga ttactatcat
      661 gaaaatgaaa tctatctcct tataagtcaa tcaatcgtgc cgtcaacatg cggatgggtt
      721 gattgttttt ctttgtatcc atcatatttt ttgattcatc tcctcttatt gaacttgttc
      781 ttaattataa aatataacaa tagaattatt tataattatt aaatttagat gcattaatat
      841 tattgatatt attttcaaaa actagaaata ttgatttgtt gcatgtataa tgttaaaagc
      901 gcccttttat aacgcttaca tataaaagct tatttaggga gagggatatt caacaagggg
      961 gatttgaaaa tgatagaact taatgcaatt acaacattat gtttagcttg tatcctttat
     1021 ttacttggta aggctatcgt taatcacgtt aattttttaa aacgtatttg tataccagca
     1081 ccagtgattg gcggcttaat ctttgctatt ttagttgcgg ctttggattc atttggcatg
     1141 gttaagatta aattagatgc ttcattcatt caagatttct tcatgttagc attctttacg
     1201 acaatcggtc ttggtgcatc attgaaatta tttaaattag gtggcaaagt cttgctatta
     1261 tactttatgt tttgtgctat catttcagtc attcaaaaca tagttggtgt atcactagca
     1321 aaagtattaa atattaaacc tttgttagga ttaacagcag gttccatgtc tatggaaggc
     1381 ggtcatggta atgctgctgc ttatggtaag acaattcaag atttaggtat tgattcggca
     1441 ctgacagcgg ctcttgcagc tgcaacttta ggtcttgtat ttggagggct tatcggtggt
     1501 ccagttgtaa aatttttaat caaacgttat aacttaaagc ctcaacatag tgacgataca
     1561 tttaaagatt atagccaagt agcatataac gaacatttac atagtaaatt taatgccact
     1621 gaagtattct tcattcaatt tacaatcgtt gtattctgta tggcagttgg aagttatttc
     1681 agtcatttgt ttacagctca aacagggatt aatgttccaa tttacgttgg ctcattattt
     1741 gtagctgtta ttgtccgaaa tatctctgaa agttttaatt ttaatattgt agatttaaaa
     1801 attactaatc aaattggcga tgtcgcatta ggtattttct tatctcttgc gctaatgagc
     1861 attcaattaa tcgaaattta taaacttgct atacctctta ttattatcgt tttagttcaa
     1921 gttgtcgtta tgattttatt tgctgtttta attttattta gaggtttagg aaaagattat
     1981 gatgctgcag taatggtagg tggttttatc ggtcatgggc ttggtgcaac gccaaatgcc
     2041 atggcaaatt tagatgttat tactaaaaaa tatggaaact cacctaaagc atatttagtt
     2101 gtacctattg ttggtgcatt cttaatcgat ttaattggtg ttatagtcat tatgggattc
     2161 atacaatggt ttagttaaac accaaactca taaataaaag aggaggcctt cgcctcctct
     2221 tttatttatc ctcgatgtat attcaagtta cgttgttcta tccatgacaa tatttccgga
     2281 ctaaatacga tttgtttttg tgttaagtcg tcaatatttt tagcatctaa catcgtcatt
     2341 attgatttca tgtgttcaat aaatgattct acataagcta ctgtatgtgc aatgccatta
     2401 ttttcaactt gatttaaaaa cggacgtgac ataccagttg cctttgcacc aagtgctaaa
     2461 cttttaattg catcgagtgg tgtacgtaaa ccaccactcg cgaaaactga aatttcgctt
     2521 tgataagccg ttgtttcaag taatgactca actgtagact gtccccatga tgataagtaa
     2581 tccatatctt tatttgcacg acgttcattt tcaatatcta caaagttagt accacctttg
     2641 ccactaacat cgacatactt gacgcctatt tgttgtaagt catgcattaa ttctttgctc
     2701 ataccaaatc caacttcttt tataatgact ggaacagaca ctcgtgatac aatcgacgct
     2761 atattatcta accaagtcac aaattcacga ttcccttcag gcataactaa ttcttgagga
     2821 gaattaacat ggatttgtaa cgcttgtgcc tcaagtaatt caactgcttc caaagccttt
     2881 tctactggta cgtccgcacc aacattgcta aaaatcatgc cttcaggatt catttttcgc
     2941 gcaatcgtaa acgtctcagc catgcgtgga tttctcaatg ccgcatgtgt tgatccaact
     3001 gccatcgcta agccagtttc tcttgcaact acagctagct tttcattgat gtttttcgtc
     3061 cactcgctac cacccgtcat tgcattaata taaaccggat atgccatcgt taagtcaggc
     3121 gtctgtgatg tcaaatcgat atcatttaca ttaattgatg ggatagaatg atgcacaaaa
     3181 cgcatcttat caaaatctga atgcattgcg tcagattggg ccattgctat ttcaacatgt
     3241 tcattttttc tctgttctct ttgaaaatca ctcatgatta aacctacctt ttcgtcattt
     3301 ctaatttaaa accatttttt acgtctgaaa aagatgatta atacgacagc tataacaaac
     3361 attacagcta agcaaatata atatccataa tgtaaatgta atgccggcat atttacaaag
     3421 ttcataccat aaatcccagc tatgaatgtt aacggtgaaa atataactga tactaatgtc
     3481 agtacttgca taatactatt cattctaaat gacgtgtatg actcaaaatt ttctcgtatt
     3541 tcgtttgtca tttcttgagc agtacgaatg atattacgtt gcttaatcaa gtggtcatcg
     3601 atatgttgaa tgtatagcga atgtttatta tctataatca aatcaccatt ttgtttcatt
     3661 gtatcaatta gctcttgcat aggaaacagt acacgtttta ctttaatcaa atccgaacgt
     3721 aacttaaaga cactatccat gaccatttta ttaaagcgat catctacatg gcggtcttca
     3781 aaatgataaa cactatcttc aagtgcatat acaaagttga aatatttatc aaccatcata
     3841 tctaaaatta atatgacgac atctgcacaa tctaattctg catctaatgt attcatatac
     3901 ttatagacta ctttatttaa tgattccaac gtttgatgat gatatgttac taatacattg
     3961 tcttgtataa aaatatttag tgctattggt gaatagtttg accccataat actatggaat
     4021 actaagtatt gataatcttt ataagattta tatttagctc gtggcatacc gttaattgca
     4081 tcatccactt ctaaatcatt aaaattaaaa tgtgctttaa accattcatt ttcttgttca
     4141 ttcggttcat caaaatcata ccaaacaata gtcgcatctt ttggtatctc tttgatatca
     4201 tcaactactt taaacggttc atatgtagtt tgataccgta tctttaaagc catcgatact
     4261 ccccctaaat aacgaattct ctattatttt atcatgaatt aaataacgtg tatgtcttaa
     4321 tttattttag tatgatagtc actaaggaga tggttattat caaacaactt tttacacata
     4381 ctcaaaccgt aacatctgaa ttcattgacc ataacaatca tatgcatgat gcaaattata
     4441 atatcatttt tagtgacgtc gtgaatcgtt ttaattacag ccacggtctt tctttaaaag
     4501 aacgcgaaaa tttagcatat acgctattta cactagaaga acatacgaca tacctctcag
     4561 aattgtctct tggcgatgta tttactgtta ctttatatat ttatgattac gattataagc
     4621 ggttgcattt atttttaaca ttaactaaag aagatggtac actagcatca acaaatgaag
     4681 taatgatgat gggaattaat cagcacacac gtcgttctga tgcttttcct gaatcatttt
     4741 caacacaaat agcacactat tataaaaatc aatcaactat cacttggcct gaacaattag
     4801 gacataaaat agcaattcca cacaaaggag cattaaaatg acagatgcat tacaacaaaa
     4861 gattcatatc gaattactag atttattaga tgatgttaag tttgaattaa cagaattaaa
     4921 tgcacaaaaa gggttataca ttaacggacc agcaaatcag ctacttaagc gtggcgtgca
     4981 tatggcttat gttcaaggac aaaagcaagc catcgataat attatgacta ttgtggaaca
     5041 acagcttgaa gatcaacatt tcctagaaca ttatgataaa tttcaaaatg aggttgctca
     5101 tcgcaattat gataaaacag ctaactttgc agaacttagt gatattccac gtcaattcga
     5161 taatttttta gatcaatttt atcaaattaa agggcaatac tttatcatca cacatatcaa
     5221 tacacttatt ggtgattttc actcagaagc tcattaacaa ttagtctata taacccttgc
     5281 tatattttca aaaacaaaac ccaattacgt tttcatgtca aatatcatct tgcatgaaat
     5341 cgtaactggg tcatttatat gttattagtt attttgtgtt acatcctcat ctatcgattt
     5401 ggcaatttgt ttaatagctt tatgtgattg tctaattgga taaattggaa aatcatgtac
     5461 catcttagga taatcataaa actcaatgta ttgatgatgt tgcaacatca tttgttcaaa
     5521 tagcttcata tcaggatgtg tcatttcacg tccaccacca aacatataaa ctggtggcaa
     5581 tccttctatt gtgccattaa ttggcgatat gcgcttatct gttaatggta ggccattcgc
     5641 ccattttttc ataatctcat tgacaccaaa ctgacttaga accgcatctt gttcgattaa
     5701 ggcgtccgaa atatctttat tagatagtgt tgcatctaaa attggtgaga ttaaatacaa
     5761 tttattcggt aatggctgtt gattatctaa aagagattgt acaaaggata atgccagtgc
     5821 accacctgaa ccatcaccca tgactacgac attttgatgt cctacttcag atactaattg
     5881 atcataaaca cgttgtatcg cttggaaagt atcgtcaata tgaaactctg gtgtctttgg
     5941 atagataggc agtacaacct catataatgt acttaaagtg attttatcca acaatctcca
     6001 atggaacggt gatggttgta gtgcattgaa tccaccgtga atatataaaa ttttcttatc
     6061 aatttgatgt ctgaaattaa agcgaaagac ttgcatatca tctaatgaca atttttctaa
     6121 atttgcttta acatttaatg ttgaaggctg cttatgtttt tttctatttt caatttctct
     6181 tttataaaaa aatctttcaa catcttgatc atttttaaac ataatcgagc gattgtgaag
     6241 caaatattta ttgacaacgc tattcataac acggtttcta atcaatgtct taacctacct
     6301 ttatatattt tatgtatcca atgattgtct atcccctaca ttctttgcca aaaaaagtat
     6361 ataatgtaga agatattttc tttttcactt tcaaatttaa gactacaatt gaacagtgat
     6421 ttttcatcat tataacagac aactagacat attgataagt aaagaaaaga actttatacg
     6481 gaggtacctt gcatgacaaa tccaaatcaa cgattagaac catttgatga gacatttcaa
     6541 caaccgaata ttcatcgtgg taagcgatat ggtaagaaaa aacgttcatt ggtaagcatg
     6601 attattcaaa tcattgttgt tatattaacc gcaatcgctg gatacagcat gtggaaacaa
     6661 cccattttta atattgtctt caccaaagaa tctgtagatt ttgatggcat taaaaatttt
     6721 aacgagacaa ttggaaaaat gcatcaatta aatattaatt tgggtaatat caatgattta
     6781 caacaaggaa tcgatcgctt aattttagtc tttaacggtt tctttatttt atgtatcgtt
     6841 agcctagtca taacaatact gacaattgta tttaatcgca cagttctaaa agtagtaaat
     6901 attttagtgt taacaattat gttagtcatt acattgtatt tcagttatat tattaaagca
     6961 ctcgcacaag aaattgcaga tgttttcaaa aaatattatt taaatgtacc accagatcaa
     7021 gtgattacag aagctgacgc tattcataac ggcatcattt tacttggctg tagcatcggt
     7081 ttacttgtta taagcttatt cttccgtaat cgcatgcctc gtttaaaata atgttatata
     7141 gtagggattg ggacagaaat gttattttca caaaattcat ttcgttgacc cacttcctgc
     7201 tttttcttta ttaatgacct actacgtcaa tgccaaattt taatataatt cctctaacat
     7261 tctataaatc aacaaataaa aaaagtatcg ataaccatat ttggcaccga tactttaaaa
     7321 atcatgtccc cacaacataa tttttgatta gtcacacttc aattaattta ttttatttat
     7381 tcatgattga tactattatc tgcatctaac ttttgagctg tagcttttgc ttttttatta
     7441 ctttgtaaaa ataaacataa aattaaggcg atgatgctaa atatcgttgc aactataaat
     7501 gcatcattaa taccttcaac cgttgctagt ttatttacaa attgtagtaa cactttcatt
     7561 gcgccttctt gtccgccata ttgtgatgct aattcatgca tatgatcttg tacaacagga
     7621 ttcgttttat ctaactcttc cccaaaagct gataagtgtt gtgtagtttg tgttgtcatt
     7681 acagtaacta agattgctgt acctatagag cctgctaatt gacgcatcgt atttaagaaa
     7741 gcattaccat gagaggcaag tcgtcccggt aacgcattaa tagctgcagt taccattggc
     7801 atcattataa atgccatacc aaatgaacga agtacataga tacccatgat tgtcatatat
     7861 ggtgtatcca tatttaattt agttaattcc catgttgcat aagtcattac agcaatacca
     7921 aagatagcta atggttttaa accaatagta tctaacaatt tacctgcaaa tggtcctagt
     7981 agacccataa ttagagaacc aggtaataat aacaatccgg aatctaatgc tgagaatccg
     8041 cgtaaatttt gtaaataaat cggtaataaa atcataccac catataaact taacattaca
     8101 accatattaa taattgttgt taatgtaaat gttgggaatt tcaatacttc taaattcaac
     8161 attggtgatt tcattcttaa ttctctaata acgaatagaa taataaagat aataccaatc
     8221 gcaaacattg tttctatctc tactgaaccc caacctttgt tgccagcttc tgagaaacca
     8281 tataacaaag caccaaaacc aatcgtacta aaaatgatac ctgggatatc agctttaggg
     8341 tttgttgtat attgatataa cttaaaccat acaaaaccaa ttaaaatagc gataatcccg
     8401 ataatgaaca taccgtaaaa catcacattc caatggtaat tttgtacaat ataacctgat
     8461 aatgttggac caattgcagg tgctaaaatc attgcgatac ccattgtacc catggcagca
     8521 ccacgttttt caggtggata aattgtaata ataacaattg aacctaatgg cattagtaca
     8581 cctgcaccaa tggcttgtaa tacacgtcca accatcatga ttgggaaatt cattgaaatc
     8641 gcacagatta atgaaccaat tgtaaagagt actaacgcaa ctaaaaataa ttttcgatat
     8701 gaatatttat taaatagata cgccgtaatt ggtattaaaa taccgtttac taacatgaat
     8761 cccgtcatca accattgccc tgttgacgca gaaatattaa attccgtatt aatttttggt
     8821 aaagcaacat ttaataatgt ttggtttaaa atcgcaataa acataccgaa taataatgcc
     8881 gctaatattt taccgcgtga aacaccttca ccaaaaataa agtttttatg ttcttttttt
     8941 attttttcat tcactttata ttcttctgat tcaggatttt tagcagcaac tgcttcctca
     9001 tccttattat tagtgaatgc ttcttgatct ttctcagacc cctttgtgta accatttaga
     9061 ctaacttggc tatgatcatc ttgattgtca acacgcaact cttcatgcgt catacgttgt
     9121 gtcgattgat cagttgtttt gtctaaatca ctagctttaa atttagattg atttgattga
     9181 cgtgtcgtaa attgttgttc cttttgttgg cgtttgcctt tttttcttga tcttattaaa
     9241 aataaattga taaccccaac aataatgagc gctaaaataa tgtagctaat aatgaaggtc
     9301 gtagtcattt aatgaccccc ttaattttta tggattttta cttcagcgtt cattccagga
     9361 acaacttgtt tagacggttc tgattctaga gtgattttaa caggtattac ttgagaaact
     9421 ttagtgtagt taccatcact atttgatgat ggcattaatg aaaagcttgc agcagttgct
     9481 tttccaatac tatcaacttt acctttaata gaagcttttt gaccgtcaat agtcacatca
     9541 acatctttac ctacttcaac atctttaata tctttttcgt caatatttgc tgttacatat
     9601 aaatcatcta aattgtatgc ataagcgatt gggttaccag cttgcaccat tgaaccttcc
     9661 ataccatcta atttggcaat tgtacctttt tgaggcattt taagatccat atctttcgtt
     9721 tcgccatctt gaccttgtac agtaacaatt gctactttgt cacctttatc gagtttgtca
     9781 ccttgtttaa cattaagtga tttaatttgt ccagatgcag gacttgctat tttaatttga
     9841 tcgccattta cttttgcatt atcagttgtt acatagcttg ttgttttatt ccaaaaataa
     9901 aagccagcaa tcccaattgc taacagtaca acaaccgtaa tgacattaat taatatcatc
     9961 tttttcatga aacatttcct cctatttgct tttaaaattc actttattta ttatagagac
    10021 atgataacta aaaaaacacc aacaattttt ataagagtgt tggttaaaat tcacaaaggt
    10081 gtaacaatat gaaacgacaa gccaaaatag aaattcaaaa cgcacttgtt gatttgatgg
    10141 ctgaatatcc ttttcaagaa atatccacta aaatgatttg cgcgtattgt aatattaatc
    10201 gttctacttt ttatgattat tacaaagata aatttgattt attggatacc atcaactcaa
    10261 agcataagga aaaattccaa tttcttctaa gcgcgctaca tcataatttc gaaaatatta
    10321 aacaggataa gcttaaactt tataaattct tcattattat tgctaagtat atcaagcaca
    10381 atgagcagtt tttcaaagac attttagtta cctatccaat gaaaacatta ttcatagact
    10441 atataaattt agcgagagat tactatcaac aaattatgaa tgactatagt actacagtaa
    10501 ccaataaaca gctttatgtt acatatatca taggcggtca agctggggtg tttattaatt
    10561 ggctttcgaa tggttgtaat gaatcacctg aagaagttgc tgacatttta ttggctaata
    10621 cgattaaact tcaaaactaa taatacggtt tcattctaat aaagaaatgc tataaaacaa
    10681 ctagataaag ttaaaccaaa tggatagata atagtacata tgtaacggtg ctatcaatat
    10741 aaaaatgccg agatttccac ataataatct cggcattttt atttttgatg ctaaattcat
    10801 aggtctatga atagctcaag t
//
LOCUS       DAKTIP010000085        10759 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAKTIP010000085 DAKTIP010000000
VERSION     DAKTIP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.085"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..553)
                     /locus_tag="O0305_001565"
     CDS             complement(<1..553)
                     /locus_tag="O0305_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274712.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIR"
     gene            complement(722..1438)
                     /locus_tag="O0305_001566"
     CDS             complement(722..1438)
                     /locus_tag="O0305_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274713.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            complement(1600..2106)
                     /locus_tag="O0305_001567"
     CDS             complement(1600..2106)
                     /locus_tag="O0305_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274714.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            complement(2242..3042)
                     /locus_tag="O0305_001568"
     CDS             complement(2242..3042)
                     /locus_tag="O0305_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274715.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTAAFINKHHDEKHV"
     gene            complement(3026..3745)
                     /locus_tag="O0305_001569"
     CDS             complement(3026..3745)
                     /locus_tag="O0305_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0274716.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            complement(3800..4750)
                     /locus_tag="O0305_001570"
     CDS             complement(3800..4750)
                     /locus_tag="O0305_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0274717.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVTGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            complement(4898..5785)
                     /locus_tag="O0305_001571"
     CDS             complement(4898..5785)
                     /locus_tag="O0305_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0274718.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSNDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            complement(6164..6799)
                     /locus_tag="O0305_001572"
     CDS             complement(6164..6799)
                     /locus_tag="O0305_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCY0274719.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            complement(7187..8848)
                     /locus_tag="O0305_001573"
     CDS             complement(7187..8848)
                     /locus_tag="O0305_001573"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCY0274720.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            complement(8845..9261)
                     /locus_tag="O0305_001574"
     CDS             complement(8845..9261)
                     /locus_tag="O0305_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCY0274721.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            complement(9354..9461)
                     /locus_tag="O0305_001575"
     CDS             complement(9354..9461)
                     /locus_tag="O0305_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274722.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            complement(9536..10546)
                     /gene="adhP"
                     /locus_tag="O0305_001576"
     CDS             complement(9536..10546)
                     /gene="adhP"
                     /locus_tag="O0305_001576"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCY0274723.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
BASE COUNT         3425 a         2030 c         1375 g         3929 t
ORIGIN      
        1 aacgaattgc caccgcacta tcatcctcag acttaatgct atatattctt tgtttttgat
       61 accatttcat cgtcttctct tgatttctaa acaatttaga aaataaaaga atatacgctt
      121 cagacttatt aaaacccgct atatcatctt gatattgtat taacaattga gtaaagctgt
      181 gtattaagtc atctttcttc gatgcaatgg tagttaatga ttttacacga taagcatatt
      241 tatctgtaaa taacttagca actaccccac ctaaatcatg tcctagaata tgtgcctcat
      301 gaatatttaa cttctccatt agaattttta aatcctcaac gtgatcgttt aaatcgtatg
      361 attcactttt agaagacttg ccatgacctc ttaaatcgta agtaagtact ttatatgatg
      421 atgctagttg atgttgcaaa tcttcaaatc cagctaaatt accatccaat ccatgaagta
      481 atattatagg atccccttta ccttcaattt gataagttaa ttggatttga ggattaattg
      541 tgactttatt cattgtgaca cctccctcaa taaactatat acccaacctt cattctatat
      601 acgcttccat tttataaaca atttttttaa attatttata aattaccaaa agttattcca
      661 acaataaaaa aactattcat ctcaattaat acaattaatc aagtgaatag ttttatcata
      721 tttataatct tgtacgtgaa gataatctaa ttgtgaaata tataactaga actactaata
      781 atatccatat aattaatgaa aaaatctggg ctgattgtgg attatgaatc attgcatcga
      841 cggttttttg acaaaataat aacccgattc ccgaaaactc aagtcgctta taaaatatgc
      901 cggcaattac taaaataata ccaatgaaaa agaaaaattg atgtccaaaa tttgtaattg
      961 gtataacgcc ccaagtaata ttaaatgctt gtaatataat taataacaag gatatgatag
     1021 cgataattgt acccataatc atttctattt tattactacg caagtaattc ttttgagaca
     1081 cccttaggta tacaacaata gtcattggaa aaatcactaa acaataaaat agactcattc
     1141 cagtaatagc ttgaaactga acatactgtt tatcatataa ataggatatg agaatgaagt
     1201 ttataataaa gaaaataact ggattcattt gtaaagcaaa caaattcctt tgaatcatta
     1261 atatgatttg tgctggagat tttcttttat attcaatata atcttgatct ttcttttcag
     1321 aattttttat atcagtaatt actttctctt taatatcatc aatcaaatgc ggtgataaac
     1381 ctttatgatt aaagtaatct tctatatttt ctagtaaaat tttatcgtta atacgcattg
     1441 gtagtactcc tttaaaatga aacatcttta tttattgtat tggtttaatt tccccttcgc
     1501 aaatcataaa aattaatatt cactgtatta tgccatgtaa taaaataacc gacaagtatt
     1561 gcgatttaac ttgtcggtta agcacgatta atttaattat tatttttcca aatcaatacg
     1621 atataatttt atgtttttat cttttggtgc tgaggcaaat tgataagtcg ttttgccttt
     1681 ttcaatatat ccaaaattac cagcaacttt gctatattcc gtatgtgtta ctgctttgcc
     1741 aacataattt ttaacagctt gatatgttgg accattagca tcattataca tcattgaaat
     1801 atgagttact ttaccattgt ttttcttacc atcagtagtt actaataaca tacctttttt
     1861 agtatggaat tcgtaataat gttcagttcc atcttcattg taagaataca tcggattttt
     1921 gtacttagtt aaaacatctt taatagattc gccaattttc acattttcta atgactgatc
     1981 acccttaatt aattgtttaa cagtatcaat agagttgcca cttgctgctt cggcactagt
     2041 gttcactaag cctactccca ttacaacaga acatgcaatt atacttgccg ttagtagctt
     2101 tttcataagc actctctcct tttatttata tcgtcttgtg ctcacaacca ttatacaaca
     2161 atcatttcta aaacaacagt catattacaa ttatattaca aataataatt actactttta
     2221 tatttcactt atcactaaaa attaaacatg cttttcatcg tgatgtttgt tgataaatgc
     2281 agctgtatct tcgacgaatc tttcttgctc ttcaacaaat gggaatgcac ttgattcttg
     2341 atatacttca aagtccgcat tttggattaa atcagctact tctttagctt ctaatcttgt
     2401 tgttctttca ccgaattctc ctgcaataat ttttgtcggt actacgacat ttctataagt
     2461 ttgagaaata tcagcgtttt tgaatacctc tttaactgct tgaatttctt ctttagttga
     2521 tatttcattt gtatctacga catgtttgag gaatcgattc atttttctcg gacgataata
     2581 cttacgctta tttaaaaatt tatcttgttt ttcaggatcc cagtttcgaa taatatgggc
     2641 atattttcta aataaacgtt cttccggtaa ttcaccttca atagatgttg gatttaccaa
     2701 cgtaagtgat gatgtaaatt caggataacg tactgaaata tccgcaccaa tgattccacc
     2761 catttcatgg catacaaatg caacttcttc aatatataaa tatttaagta attcaacaat
     2821 gtcatcagaa aaatctttta tttcaatgtg acgaggttta tcagaatagc catgtccacg
     2881 taaatcaatt aacacaactt gaaatgattt tgctaattct gctgctaatt tattaaacac
     2941 agaataatta tcaagtacag tatgaatcaa tacgatagga tagccttcac ctaatgtact
     3001 gtaatgtatc gatgttccat cttttctagt aaatagatcc ataattttct cctttattat
     3061 cattttttat tggtaaaatg gttaataatt cttccactgt tgtaattgta aaatcaactt
     3121 cttcttcgag tggttcaatt tcagcatctt cttgtttata ccatacacta accatgccca
     3181 tagctcgtgc tggagctaca tcatttaacg catcatcgcc aacatacata atttgctcag
     3241 gtaatacccc tagttgatca atcatatctt caaaaatttt aggatgtggt ttacgaaaac
     3301 caactgtttc tgatgttgat aaataattaa taacatgcat caaaccaagt gaatgtaatc
     3361 gaaattgttt aatcttagat ttaccatttg cgataacacc aatttgaaag cctttttgcg
     3421 atagcttttc tagtgtatac aaagtatcgt aataaggaaa tacataacga taaaaatgca
     3481 tttcaaaatc attaaataag tctttccaag ttaacctatc tacatggaaa cgtttaatga
     3541 tttctttata taaatcaggt ttatcattat cttcatcgtc atctagctca ataaatgctt
     3601 ttttaaaatc tgccagttga acatgaatta agtagtcatg aaatcgttca tattgctctt
     3661 cgataaattt ctctcgagat ttaactctgt ccaacaacgt accttctaaa tcaaatacaa
     3721 ctgctttaat ttgattcaaa tccatcgctt atgccctctt tatgtttata gttattttta
     3781 ttttagcaaa tgtcgtgtgc tagtgtaacc gtttcgactg agatattagc atgtaaaata
     3841 acactggtat accaattaat gcaatgataa tacttgccgg tatttgtact ggtgacaata
     3901 tatggctacc aataaaatct gccataacca ttatttgtcc tccagttaca atatttaaag
     3961 ccaattgtct tcctaatgat tgtttccact gccattttct aatgagttgc ggtatcacca
     4021 taccaatgaa tccaatgatg ccaacatatg cgacatttat cgccgttatc atagaagcta
     4081 tacataacgc gatatatgta atcaattgaa catttaagcc tagtgactga ctttttagtt
     4141 ctcctaattg caataacttt agttgattaa gaatgataaa tataccaaac aatgcaatga
     4201 taaatgtgat tgctattata gatacatttg agtattctgc atcaccaaaa ccaccaaaca
     4261 gataattggc aattgtattt aatttgcgag gttttaataa aatcaaaaaa tatagaagtg
     4321 aattgaataa cgcaccaatc attaaaccac ttaatattaa gattctaact ggatagcctt
     4381 ggctcaatac cgacgtaatg actaatacag ttattaatgt tatcaaacta aatgttattg
     4441 aaaatacagg aatccataac gttgttaaac ctaaaaataa tgctaatcct gaaccaaatg
     4501 tagcgccgct tgccaatcct aatgtaaagc tatctgccaa tgcattattt aaaactgttt
     4561 gaaatataag gcctgcaaca gttaatatca tacctgtcaa caatgcttct aataatctcg
     4621 gtaatctaac attgattaaa atagactggt taaatacatc attgatatcg cccaactgcc
     4681 aaaatagata tatgctagtt gttataatca atatgactat ccagctcaat aatactttat
     4741 taaatgtcat agcattctct tttctttcat cgataattac attcattata gcaatcacat
     4801 actcgcgttg ctatcattca ctaaatatat tttaaaaacc ccttatctca aacatgtatt
     4861 ttttacatgt agcaataagg ggcataatta gaatggttta ttttctataa attgcatctc
     4921 ttaattcttt taatccttca tcaatacgtg gacctggtct tgatacttca tcaccattta
     4981 caacttcaat acgtgtattc ttgacagcat taattttatt gaatccacct ctttttttga
     5041 tgatatccat ataatctgat cttgtcttag cttccgtcga aattaatata tctgggttct
     5101 ttttaataat actttccttc gttacagggt tccaaccatt aatgtcacta tacacatttt
     5161 gggcttctaa tttttctaac atatcattaa aaaatgtatg cttccctgct gtatatattt
     5221 caggctttga tgaaacctca ataaatactt ttgatttttt atgatgagca ggaattgaat
     5281 ctatgacttt atcgatatta tctttagttt cctcaacaag ttgttcagcc tgcttatcat
     5341 gatgcgttaa tttcccaatt tgcttaaatg tgttgtaagt ttcatcaatt gattgtgcat
     5401 ctttaacata cactactttg atgccttgtt tctctaatga tgacaatact ttattagcag
     5461 ttgccttttg cgactcatgc gcaagaatta gatctggctt tgcctttaaa agttcctctt
     5521 tatttagatt caaagcatca aattgtttct tacccttttt cacatctttt ggataatcat
     5581 caaccgttga aacaccaact atgtatttac ctaatcctaa ttcatataaa atttcagtat
     5641 tactaggcat taacgaaaca attctatggt acggcgattt agacgagcca ttatcattgc
     5701 taccttgttt atcattatct ttcataccac agccactcag gacaagcata aaaatcaaaa
     5761 taaaagcaat taacgatttc ttcacactat cacatctcct ttttaatccc acttttcgct
     5821 ttagcgatta gagggattta tgcagttgtg cggtagcatc caactgcgtt cctcttcatc
     5881 tttagcgatt agaggctttt atgcagttgt gccttagcat ccaactgcgt tcctcttcac
     5941 ctttagtgat tagaggcttt tatgcagttg ctgcacaaac aactgtattc ctttactccc
     6001 cttgagcttt agcgattagg ggctcttatg cagttgctgc acaaacaaac aactgttttt
     6061 ttctttatat gcttctattt attatataag tccacatact aaaagaaaat agtaaattat
     6121 tttttatatt tcatataaac tcatctatct aacttcacag cgtttacttt ttaaagtaag
     6181 gttctaaaaa atcataattc tttatatctt tgtctctaaa gtaatgttta ccaaatacat
     6241 ctaacagagc atgaaattca ttagcatctt gatttgtaaa atgatttggt aatgtgacta
     6301 ctttttttaa ttgatcataa tttttagtgt tttcatatcc taatttatca tatatttttc
     6361 ttgtatagct atctggaata aattcaatac gtccgaatat ataaacaagt aagacatctg
     6421 ctgtttcact tccaatacct ttcaaagata ataattcttt tcttaatcca cctttatatc
     6481 gctcattaat ctcttgataa ttgaaatgat gtcgtgctaa ccatgttaat aatgttttaa
     6541 tcgtcagtga tttactttta taaaagccac ttgaatgtat caatgattgt aacgtttcaa
     6601 taggtagttc taatatatga tttggattaa aatgcgtatg ttctttaatc tgattcaatg
     6661 caatttctgc atttcgccat ctagtatttt gaactaaaat tgcacctaac atcatttcaa
     6721 tgtcattatc agcaggccac caaaattgtg gtccgagatg ttcatataaa actttatata
     6781 attcatcagt tcctaacatt tacaccctcc attttagagg gagtgggaca gaaatgatat
     6841 tatcgtaaaa tttatttcgt cgtcccaccc caacttgcat tgtctgtaga aattgggaat
     6901 ccaatttctc tttgttgggg ccccgccggc aaggttgact agaattgaaa aaagcttgtt
     6961 acaagcgaat tttcgttcag tcaactactg ccattataac tttgtagagc atagaacatt
     7021 gatttatgtc tcaggctctc ttatgcagtt gtgcagagca tccaactgtg ttcctcttaa
     7081 ccttattcac atactaagca ttccccaata tgtcacatac ctaaaaaaac actccaacac
     7141 aaagtatctt tcgagaaact ctacgttgga gtgtatataa atgttcttac attgattcag
     7201 gtgcgcttac accgaccatt gccaatgcat ttttcaatgt aattctgacc gcttcaatca
     7261 tagcaacatg tgcttttgtt ttttcaatat catctgttaa cactttttca gcattataga
     7321 atttatggaa atgagaagct aaatcttgaa tataattagt aattctatgt gccgatctat
     7381 gctcagcagc actttcaatt gtaggttcga aatcagctac ttttttcaac aattcaatcg
     7441 ctttttcatt agtaatcgtt gtaaaatcat tcgcagcagt cacttcaata ccttgctctt
     7501 tcgcttgttt taaaattgaa caaatacgcg catgtgcata ttgagcatag taaactggat
     7561 tgtcttgaga ttgctctttc gctaattcca tatcaaaatc aaagtgacta tcaggactac
     7621 gcatagttaa gaaataacgt gcagcgtcaa cgccaacttc gtccataatt tctcttaatg
     7681 taatcgcatt accagtacgt ttactcatct tcacttcttt accattttcc attaaacgaa
     7741 ccatttgcat gatttgaatt tctaaacgat tactatctac accaaacgtt tcaagagatg
     7801 ctttcaaacg attaatataa ccatgatgat cagcaccaaa taaatcgatt aaaatgtcat
     7861 taccacgttt tactttatcg aagtggtacg caatatctgg taagaaatac gtatatgtac
     7921 cgtcattttt aattaatact ctgtctttgt cgtctttaaa atcagttgta cgtaaccatg
     7981 tagcgccatc agcttcatac gtataaccta attctttcat ttttgctaaa acttcaagaa
     8041 tttctccttt ttcatataaa gatgtttcac taaaccaatt atcaaaatgc gtattgaact
     8101 ctgctaaatc atttttcaat ttagccattt cgtattctac gcctaatttt ctaaattctt
     8161 tcaaacgtgc ttcttcagaa taatctttaa tttcagggtg tttctctgct aaatctttac
     8221 ctatttcaat aatatctttt ccattatagc catcttctgg cattgaataa ctattgtcac
     8281 ctaaagcttc aaagaaacgt gtttcaatcg aacgcgctaa gttagtaatt tgattaccag
     8341 catcattaat ataatattca cgtgttacat tatagccagc tgcagttaaa atattagcta
     8401 aagcatcacc aactgctgca tttctagcat gaccaatatg taaatctcct gtagggttag
     8461 ctgaaacata ctcaagcaat acattttgac cttttgattc atttacatgt ccaaattgat
     8521 cacctttttc aattgcttca ggaataattg ctgttaaata ctgattatct aagtaaaaat
     8581 taatgaatcc tggaccagca atgtcaattt gttttacatg tgctttttca gtatctaagt
     8641 tatcaacaat cgcttgagca atttcacgag gattacgctt tgcaatctta gtcagtacca
     8701 tcgcaatatt agtagcataa tctccatttt ttgtatcttt aggaacttca attttaatat
     8761 caggaatctc atctgctaat cctgctttgt taatacttgc tgcaatttct tctactaatg
     8821 tttgtttcac ttgatcaata atattcattc gcctatctcc ttatagttaa tttcatattg
     8881 ataagaaccc attttttcat tatcttgata taattcataa tgaatcttta gcttgccacc
     8941 gtctccactt acgaaatgta aaatgcgtaa tgttttaact tctagtggaa ttcgtccagc
     9001 cgatatatcg taaaaagttg ttgtcgtttg tccttcaaca aaatgcaaat tcatattaat
     9061 gtcgccttta cgaatcaact taacgctatc atcatccact tttattgtaa cattaactgt
     9121 tgcatcctca atttgttcta cataccgaat aaagttagat tgcctttgtt gccaagttcc
     9181 ttcagtagta aattcaaatt tttctttttc gttgtgctgt ttcaacactt gctttgtttg
     9241 aatacttact tttttatcca atttactact caccttaatc ttcgcataat agaaatatta
     9301 tagcataaaa taaggtcatc ttgttatgcc ttcttaaagc taaataggtt caattaatta
     9361 acaattccat acttaatcaa cgcttcatat atgtaagcca ttttataaaa tacaaacaac
     9421 acctcaaaac caaaaaacac gagattttca ctatctttca tgaaaacctc gtgtttgatt
     9481 ataatgatag tgttcagagc actaccttct ttatctatat agtcagttga tatttttaaa
     9541 atttaataac cattctacca gttatagtac cattttccat ttcttcaaaa atatcattga
     9601 tttcttctaa ttttcttaat tgaactttag gtgttacttt attttcagca gcaaattcaa
     9661 acgcttcacg taagtcttgt cttgtaccaa caagtgaacc tactacttca ataccatcaa
     9721 gcactaatct tgggatatct aagttcattt tatcaacagg taaaccaacg gcaacaactc
     9781 ttgcaccagc ttttacaaca tcaaccgctt ggttaaatgg cgtcttagca actgaagtta
     9841 ccactgttgc atctaatcct ttgttatcag ttaatttcat aacttctgca actggatcaa
     9901 catctttaga attaataata gcatcagcac ctaattcttt cgcgaatgct aatttatcat
     9961 cattgatgtc gaaggcaaca attttagccc ccataacgtt tttagcatat tgtaaagcta
    10021 ggttacctaa accacctata ccaaaaacac ctaaccattg tccaggtttt acattactta
    10081 cttttacagc tttataagtt gtcacacctg cgcatgtaat agaagacgct gctgctggat
    10141 ctaatttttc aggtactttc acagcatagt ctgcagtaac aataacttgt tcagccattg
    10201 caccatctac tgtataacca gcatttttca cactacggca aagtgtttca cgacctgttg
    10261 tacaatattc acatcttcca cagctttcga acatccaagc gatagacaca cggtctccaa
    10321 tttttaatga ttctacatct tccgcaactt cgatgacttt accaatacct tcatgaccta
    10381 aagtaacgcc tgtaacatca ccaaaatcag cattcttaac atgtaaatcg gtatgacaaa
    10441 cgccacaata ttccgtttgt accaacgctt caccaggttt taaagctctt aactttttgt
    10501 cctcaatact tactttgtga tctttcgtta caactgctgc tctcataata atgtcctcct
    10561 tatagtcact ccagtatgta tgatattaat tggctattac tttcaaaaaa gaaaacgaat
    10621 aattatagat gtaattttta tttgtaatag acgccagatt aaaacatact actgtgacct
    10681 tgtttttagt tagatatcaa ccgttaactt ttaacgtttc cctagttaat atgttaaaag
    10741 aaatgacata atcgatatg
//
LOCUS       DAKTIP010000086        10721 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAKTIP010000086 DAKTIP010000000
VERSION     DAKTIP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.086"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            107..1336
                     /locus_tag="O0305_001577"
     CDS             107..1336
                     /locus_tag="O0305_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0274724.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            1591..1683
                     /locus_tag="O0305_001578"
     CDS             1591..1683
                     /locus_tag="O0305_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274725.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
     gene            1861..2694
                     /locus_tag="O0305_001579"
     CDS             1861..2694
                     /locus_tag="O0305_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCY0274726.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            2976..3773
                     /locus_tag="O0305_001580"
     CDS             2976..3773
                     /locus_tag="O0305_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274727.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            3809..4813
                     /locus_tag="O0305_001581"
     CDS             3809..4813
                     /locus_tag="O0305_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0274728.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            4810..5826
                     /locus_tag="O0305_001582"
     CDS             4810..5826
                     /locus_tag="O0305_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0274729.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDKLILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            6060..7028
                     /gene="dhaK"
                     /locus_tag="O0305_001583"
     CDS             6060..7028
                     /gene="dhaK"
                     /locus_tag="O0305_001583"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCY0274730.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            7070..7654
                     /gene="dhaL"
                     /locus_tag="O0305_001584"
     CDS             7070..7654
                     /gene="dhaL"
                     /locus_tag="O0305_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCY0274731.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            7647..8009
                     /locus_tag="O0305_001585"
     CDS             7647..8009
                     /locus_tag="O0305_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCY0274732.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            8125..8622
                     /locus_tag="O0305_001586"
     CDS             8125..8622
                     /locus_tag="O0305_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274733.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            9078..10145
                     /locus_tag="O0305_001587"
     CDS             9078..10145
                     /locus_tag="O0305_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274734.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            10296..>10721
                     /locus_tag="O0305_001588"
     CDS             10296..>10721
                     /locus_tag="O0305_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274735.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFI"
BASE COUNT         3575 a         1478 c         2067 g         3601 t
ORIGIN      
        1 tcgtatttta catagcaatt taactgattt tagagtccac ggtacagaag tttgatattt
       61 caatgtttct aaatttttaa aaaattaaat cataggtggg tgccaaatgt ttttattaat
      121 caacattatt ggtctaattg tatttcttgg tattgcggta ttattttcaa gagatcgcaa
      181 aaatatccaa tggcaatcaa ttgggatctt agttgtttta aacctgtttt tagcatggtt
      241 ctttatttat tttgattggg gtcaaaaagc agtaagagga gcagccaatg gtatcgcttg
      301 ggtagttcag tcagcgcatg ctggtacagg ttttgcattt gcaagtttga caaatgttaa
      361 aatgatggat atggctgttg cagccttatt cccaatatta ttaatagtgc cattatttga
      421 tatcttaatg tactttaata ttttaccgaa aattattgga ggtattggtt ggttactagc
      481 taaagtaaca agacaaccta aattcgagtc attctttggg atagaaatga tgttcttagg
      541 aaatactgaa gcattagccg tatcaagtga gcaactaaaa cgtatgaatg aaatgcgtgt
      601 attaacaatc gcaatgatgt caatgagctc tgtatcggga gctattgtag gtgcgtatgt
      661 acaaatggta ccaggagaac tggtactaac ggcaattcca ctaaatatcg ttaacgcgat
      721 tattgtgtca tgcttgttga atccagtaag tgttgaagag aaagaagata ttatttacag
      781 tcttaaaaac aatgaagttg aacgtcaacc attcttctca ttccttggag attctgtatt
      841 agcagcaggt aaattagtat taatcatcat cgcatttgtt attagttttg tagcgttagc
      901 tgatctattt gatcgtttta tcaatttgat tacaggattg atagcaggat ggataggcat
      961 aaaaggtagt ttcggtttaa accaaatttt aggtgtgttt atgtatccat ttgcgctatt
     1021 actcggttta ccttatgatg aagcgtggtt ggtagcacaa caaatggcta agaaaattgt
     1081 tacaaatgaa tttgttgtta tgggtgaaat ttctaaagat attgcatctt atacaccaca
     1141 ccatcgtgcg gttattacaa cattcttaat ttcatttgca aacttctcaa cgattggtat
     1201 gattatcggt acattgaaag gcattgttga taaaaagaca tcagactttg tatctaaata
     1261 tgtacctatg atgctattat caggtatcct agtttcatta ttaacagcag ctttcgttgg
     1321 tttatttgca tggtaatatg tcgaagagtg actatgataa tacattttaa ctaataaata
     1381 tgtccaggca tgtcgtctat tgatataggt gagatgcttg gactttttta ttattgatat
     1441 aaaggtattt aaatattttt aaagttaccg aaattgaagc attataaaaa ccaagtgcac
     1501 atggtaatac acttggcttt tatgggaaat gaatattatt gtacatatga cagtaaggac
     1561 taggtacagt catagtactt cgagcaaaat ttgttttgtt attataaaca acacaaagga
     1621 gataacttct ctattgaaga agttaaaaac attatagcag acaatgaaat gaaagtaaat
     1681 taaaaattca gaatattttt aattattata ttgtgagtga tatttattag ggaaagctat
     1741 tcttcatata aattagttaa atagtaaatc tttgttagaa ctctttccat agagttgtga
     1801 cagcattttt gttgtgctac tatttttata gtctaataaa tatataaagg ggatggtttc
     1861 gtgaataaaa cggttaaaga tttaatacta gttgtcttag gttcatttat ctttgctgca
     1921 ggtgtaaatg catttattat ttctggtaac ttaggtgaag gcggggttac aggtttagca
     1981 attattttat attatgcgtt tcatatttca ccagccatca ctaacttctt ggtcaacgca
     2041 gtattgattg ccataggtta taaatttttg agtaagagaa gtatgtactt aactattctt
     2101 gtaacaattc ttatttcaat atttttgagt ttaacagaat catggcaagt agaaactgga
     2161 aacagcattg tgaatgccat ttttggtggt gtaagcgttg gactaggaat cggagtaatt
     2221 atccttgcag gcggtacaac agcaggtaca acaattttgg cgagaattgc aacgaaatac
     2281 ctcgatgtaa gcacgccata tgctttgctt ttcttcgata tgatcgttgt tgcaatttca
     2341 cttacagtta ttccacttga taaagtatta gtaacagtaa tatcacttta tataggaaca
     2401 aaagtgatgg aatatgtcat agaaggttta aacactaaaa aagctatgac gattatttca
     2461 actaatcccg acaaacttgc caaagcaata gacgagcaaa ttggaagagg tttaaccatt
     2521 ttaaacggac atggctatta tacgcgtgaa gaaaaagatg tcttatacgt tgttatttct
     2581 aaaacacaag tttcaaaagc aaagcgatta attaaacaaa tcgataaaga tgcattcctc
     2641 gtaattcatg atgtaagaga tgtctatggt aatggctttc ttgcagatga ataaataaat
     2701 ggtatgagca cacatactta aatagaagtc cacggacaag tttttgaact atgaagactt
     2761 atctgtgggc gttttttatt ttataaaagt aatatacaag acatgacaaa tcgagctatc
     2821 caatttaaaa agtaatgtta gtcaataaga ttgaaaaatg ttataatgat gttcatgata
     2881 atcattatca attgggatgt ctttgaaaat tgataattta aaaatagaaa ttatttttta
     2941 taaacagaaa gaattttatt gaaagtaggg aaattatgaa tcgtttgcat ggacaacaag
     3001 ttaaaattgg ttacggggat aacacgatta taaataaatt agatgttgaa ataccagatg
     3061 gcaaagtgac gtcaatcatt ggtcctaacg gctgcgggaa atctactttg ctaaaggcat
     3121 tgtcacgttt attggcagtt aaagaaggcg aagtattttt agatggtgaa aatattcata
     3181 cacaatctac gaaagagatt gcaaaaaaaa tagccatttt acctcaatca cctgaagtag
     3241 cagatggctt aactgttggg gaattagttt catatggtcg ttttccacat caaaaaggat
     3301 ttggtagatt aactgctgag gataagaaag aaattgattg ggcaatggaa gttacaggaa
     3361 ctgatacatt ccgacaccgt tcaatcaatg atttaagtgg tggtcaaaga caacgtgttt
     3421 ggattgcaat ggcattagca caaagaactg atattatctt tttagacgaa ccaacaacat
     3481 atttagatat ctgtcatcaa ttagaaatac tagaattagt tcagaagcta aatcaggaac
     3541 aaggttgtac aattgtcatg gttcttcatg atatcaacca agcgattcgt ttctcagatc
     3601 atcttattgc gatgaaagaa ggggatatca tcgctacagg ttcaacagaa gacgtattaa
     3661 cacaggaaat attagaaaaa gtttttaata ttgatgttgt tttaagtaaa gatcctaaaa
     3721 ctggaaaacc tttactggta acttatgact tatgtcgcag agcttattct taattaagta
     3781 agttaatatg ataaaaagga caattaacat gacaaataga gagaacccaa cgccattgaa
     3841 gtttttatcc tatattatag gtttaagtat gatactacta atcacactat ttatttctac
     3901 attaataggt gacgccaaaa ttcaagcctc tacaattata gaggctattt ttaattataa
     3961 tcctagcaat caacagcaaa acatcatcaa tgagattagg attcccagaa atatagcagc
     4021 agtaattgta ggtatggcgc ttgcagtttc tggtgcgatt atacaaggtg ttactcgtaa
     4081 tggtcttgct gatccggcgc tcataggttt aaattcaggt gcttcatttg ctttagcatt
     4141 aacatatgca gttttaccaa acacttcatt tttaatattg atgtttgctg gatttttagg
     4201 tgctattcta ggaggtgcta ttgtattaat gataggccga tctagacgtg atggatttaa
     4261 tccgatgcgt attattttag cgggtgcagc agtaagtgct atgttaacag cgctaagtca
     4321 aggtattgca ttagctttta gactaaatca aacagtaaca ttttggactg ctggaggcgt
     4381 ttcaggcaca acatggtcac accttaagtg ggcaattcca ttaattggta ttgcgttatt
     4441 cattatatta acaattagta aacaacttac cattttaaat cttggtgaat cattagctaa
     4501 aggtttaggt caaaatgtaa caatgatcag aggcatatgt ttaattattg ctatgattct
     4561 agcaggtatt gcagttgcta tcgctggaca agttgcattt gtaggtttga tggtacctca
     4621 tatagcaaga tttttaattg gaactgatta tgctaaaatt ctaccattaa cagccttgtt
     4681 aggtgggata ctcgtgcttg ttgccgatgt gatagcacga tatttaggag aagcgcctgt
     4741 tggtgcaatc atttcattta tcggtgttcc ttacttttta tatttagtta aaaaaggagg
     4801 acgctcaata tgattagttc aaataataaa cgcagacaat tgatagcact ggctgttttt
     4861 agcattctac tatttctagg ttgtacttgg agtattacct caggtgaata caacatacct
     4921 gttgaaagat ttttcaaaac tttaattgga caaggtgatg ccattgataa gttaatctta
     4981 ttagatttca ggttacctcg gatgatgatt actattttgg ctggcgcagc gcttagtatt
     5041 agtggtgcaa tagtgcaaag tgtcacaaaa aatccaatag ctgaaccagg tatattaggt
     5101 attaacgcag gtggcggatt tgcaatcgca ttatttattg caattggtaa aattaatgct
     5161 gacaactttg tttatgtact gccgttaata agtatactag gtggtatcac cactgcattg
     5221 attattttta ttttcagttt taataaaaat gaaggtgtta cacctgcgag tatggtatta
     5281 ataggtgtag gtttacaaac agcattatat ggtggctcaa ttacaattat gtcaaaattt
     5341 gatgataagc aatctgattt catcgctgct tggtttgcag gtaatatttg gggtgacgaa
     5401 tggccatttg tcattgcatt tttaccgtgg gtgttgatta ttattcctta cttactattt
     5461 aaatcgaata cactaaatat tattcatacg ggtgataata ttgcacgagg tctaggtgta
     5521 aggttaagca gagaacgttt aatattattc tttatcgcag tgatgttatc atctgctgct
     5581 gtagcagtag caggttcaat ttcgtttatc ggattaatgg gtccgcatat tgccaaacgt
     5641 atcgttggac cacgtcacca gttgttttta ccaattgcca ttttagtagg ggcatgttta
     5701 cttgttatag ctgatacaat tggcaaaatt gtattacaac caggtggggt tccagcaggt
     5761 attgtcgtag caattattgg tgcaccgtat ttcttatatt taatgtacaa aacgaaaaat
     5821 gtatagtgtc aatggacaca acttattgct atgaaaggca ctttattata aggcttttca
     5881 tagcattttt tatttaatga gccactcaag actatttatt ttttcaataa tgaaccatta
     5941 agttatcaag aggatcttat caaaaatata tttgataacg gtatcaggtt aattctttat
     6001 gatagcgcat tcatttattc tgttttatac tatgactgat aataccaagg aggtacaaca
     6061 tgatgaaaaa gttaatcaat aaaaaagaaa catttttaac tgatatgctt gaaggattgt
     6121 taattgcgca cccagagtta gatctgattg ctaatacagt tattgtaaaa aaagctaaga
     6181 aagaacatgg tgtagcaata gtctctggag gtggaagcgg acatgaacct gcgcatgccg
     6241 gttttgttgc agaaggtatg ctagatgcag cggtttgtgg cgaagtattt acatcaccta
     6301 cacctgataa aatattagaa gctattaaag cagtagatac tggtgatggt gtattactag
     6361 ttgtaaaaaa ctatgcaggt gacgtgatga atttcgaaat ggcacaagag cttgcagaaa
     6421 tggaaggtat aaatgttcaa actgttattg ttcgtgacga cattgctgtg acaaacgaag
     6481 tacaacgtcg tggtgttgca ggaacagtgt ttgttcataa gcttgccggt tatcttgctg
     6541 aaaaaggtta ttcattaaca gagataaaat cgcgtgtaga agcgttgtta cctgaaatta
     6601 aaagtattgg tatggcaatt gagccaccgc ttgttccaac tactggaaaa tatggctttg
     6661 atattgaaga cgacaaaatg gaaatcggta ttggtataca tggtgaaaaa ggtattcata
     6721 gggaagaagt aaaggatatt gatcatattg ttggaacatt gttagacgaa ttgtataaag
     6781 aagttactgc caatgatgtc atattaatgg taaatggtat gggtggtacg ccgttatctg
     6841 aattaaatat cgtaactaaa tatattcaac aaaatttagc tgcaagaacg gttaatgttg
     6901 ctaaatggtt tgttggtgat tatatgacat ctttagacat gcaaggtttt tctataacta
     6961 tcgtgcctaa taaaccagaa tatttggaag catttttagc accaacaaca agtcaatact
     7021 ttaaataaga agtgaatatg aataaatata catttatgag gtggcacaaa tgaaagtgaa
     7081 tgatatgaaa gcacgtttat taaatttaga agaaacgttt aaaaaacatg aatctgaatt
     7141 aactgaatta gatcgagcaa ttggtgatgg tgaccacggg gttaacatgg ttcgtgggtt
     7201 tagtagtctt aaagacaaac ttgatgatag ctcaatgcaa tcattgttca aatcaactgg
     7261 tatggcattg atgtcaaatg ttgggggtgc atcaggacca ctgtatggct ttagctttgt
     7321 taaaatgtct gcagtcacca aagatgatat ggataatcaa gatttcatta cactaattca
     7381 ggcatttgcc gaagcggttg aatcacgtgg taaagttact ttaaatgaaa agacaatgta
     7441 tgatgtagta gcgcgagcag cagagaagct taaaaatggt gaaactttaa cattcaatga
     7501 tttacagcaa ttagcagata atacaaaaga tatggtagca acgaaaggta gagctgcata
     7561 ttttggagaa gaatcaaaag gttatattga tccaggtgct caaagtatgg tttatatttt
     7621 aaacgctttg attggagatg aagataatgc ctaaaattat acttgttagc cacagtaaag
     7681 aaattgcaag tggtacaaaa tctttgttaa agcaaatggc aggtgacgtt gatattatac
     7741 caatcggggg attaccagat ggttcaattg gaacttcatt tgatatcatc caagaagttt
     7801 tgactaaatt agaggatgat gcattgtgtt tttacgatat tggatcttca gaaatgaatg
     7861 tagatatggc aattgaaatg tatgatggta atcatcgtgt gttaaaagtt gatgcaccaa
     7921 ttgttgaagg cagttttatc gcagcagtaa agctatcaat cggcggttca attgatgatg
     7981 cattagcaga aatcaaacaa tcattttagt taaaatttac taataatgaa aaatgtaaac
     8041 ctttttcaaa tgaaacttta taaaaaatat gatagtatat atgtaaatgt ttaataaaat
     8101 ctggagaaat aggaggacat tgccatgcaa caccttataa aaaaacatgt attgaatggc
     8161 gagtttgatt tagtacgaca attgatgtcc gaaacagatt ttatggaatt tgaagaagca
     8221 tatatttcaa gtgcgcatga agtagaaagt atgatgtttt atacatgtat tttagatatg
     8281 attaagtacg aagaatcatc tgaaatgcat gacttagcat ttttattgct tgtgtatcca
     8341 ctaagtgaat atgaaggtgc tttggattct gcttattatc atgcagacgc ttccataaaa
     8401 cttactgacg gcaaagaagt taaaagtttg ttacaaatgt tattattgca tgcgatacca
     8461 acacctgtta tttcagataa gaaggctttt gatatcgcca agcaaatttt aaaattagat
     8521 cctaataata atgttgctcg taacgtctta aaagacactg ccaaacgtat ggacaacgtt
     8581 gttgttgata taaatgaatt acaccaacgt aatgcacgtt aattacattt caattatatt
     8641 agcttaataa tagttttaac atttggttgg gttgggcata tgttccagcc ttttttaata
     8701 cttaaaaact aacgaagtat acttgtgtgc acaaatggtt tttatacaac attttataaa
     8761 tttatacatt ttaataaaga acatacgata gatggtttaa accttgttaa ctgagaaatt
     8821 ttgatatgta ttcttcgaaa tttaactaaa tatacgaaat tcaagaagca caataattaa
     8881 tcatttttcc tatacaaaag ttcgtatgac tgcattataa aagcataaat ttataatttt
     8941 tttaaatgtc attgaacgtg ataatgtgaa tggattgagc aattttgaaa aagtgaaaaa
     9001 taacctatgc ggcttgcaat taattttcag tacgttataa tgcacactgt gcaaaattaa
     9061 ggaggtctat tattcacatg atgatgaata aagaagcaac aaaaattgga tttgcctacg
     9121 tcggcattgt agtgggcgca ggattttcaa ctggacaaga agttatgcaa tttttcacta
     9181 aatatggctt gtgggcttat ttaggtgtta ttatatctgg ttttatttta gcttttattg
     9241 ggcgccaagt agcaaaaatt ggtactgcct ttgaagcgac aaatcatgaa tcaacattac
     9301 aatacgtatt cggtgaaaag tttagtaaag tctttgatta tattttaatc ttcttcttat
     9361 ttggtatagc tgtaaccatg atagctggtg caggcgcaac atttgaagaa agttataaca
     9421 tacctacatg gctaggtgct ttaattatga cattagcgat ttatattacg ttgctattag
     9481 actttaataa aatagtacgt gcactaggta tcgttacacc atttttaatt gttttagttg
     9541 tattaatcgc tggcgtttat ttatttaaag gtcatgtttc attagcagaa gttaaccaag
     9601 tagtgcctga agcaagtatt tggaagggaa tctggtttgg tacaatatat ggtggattag
     9661 ctttttctgt aggttttagt accatcgtag caatcggtgg ggatactgaa aagcgtacag
     9721 tgtcaggtgc aggcgcgatg tatggtggta ttatctatac tgtattacta gcattgatca
     9781 actttgcatt gcaaagtgaa tatccaacta ttaaaaatgc ctcaattcct acattgacgt
     9841 tagcaaataa tatccatcct ttaatagcaa cagtgttatc tgttattatg ctggcggtta
     9901 tgtataatac tattctagga ctaatgtatt catttgcagc acgttttaca gaaccataca
     9961 gtaaaaatta tcatatcttt attattataa tgatggtagc aggttattta ttaagtttcg
    10021 taggatttgc tgaattaatt aataagttat atacaattat gggatatgta ggcttattta
    10081 ttgtagtagc tgtaattatt aaatatttca aacgtaaaaa tgcggataaa aaacatattg
    10141 cttaatatca tatgagggat atccgaaact ttacaattga atcactttgt tttaacctta
    10201 aaagcaattc gtctctactc ttatcgggcg aattgctttt tatatttatt cagtctatta
    10261 atatgagcgt ctaacaaata gagaggtacg atgtaatgaa taaagataat aaatggacga
    10321 tgataactgc gctttttata actgtaatca gtgtattgtt agcatttcat ctgaaacaac
    10381 attatgacca aattacaaat gagaaccatg ctaataaaga caaaattaat attaaaaata
    10441 aaaatgtgcg catttatcaa aaccttacat acaatagagt tttccctaac agtaaattag
    10501 atattattac acctgttgat atgtcttcta atgccaaact gccagttatt ttttggatgc
    10561 acggtggtgg ttatattgcg ggtgataagc agtataaaaa cccattatta gcgaaaattg
    10621 ctgaacaagg gtacattgtt gtgaatgtaa attatgcatt ggcgccacaa tataaatatc
    10681 ccacaccatt aattcaaatg aatcaagcaa ctcaattcat t
//
LOCUS       DAKTIP010000087        10663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAKTIP010000087 DAKTIP010000000
VERSION     DAKTIP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.087"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            354..1106
                     /locus_tag="O0305_001589"
     CDS             354..1106
                     /locus_tag="O0305_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCY0274736.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTEIEL
                     TAVKTKKKIVTIQELEVQLRNYLNEKYKLYEQGGDIVKGYVKYYNDDEQNVEYDFYNL
                     NGEYGREVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(1308..1577)
                     /locus_tag="O0305_001590"
     CDS             complement(1308..1577)
                     /locus_tag="O0305_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274737.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            1891..2577
                     /gene="mtnN"
                     /locus_tag="O0305_001591"
     CDS             1891..2577
                     /gene="mtnN"
                     /locus_tag="O0305_001591"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCY0274738.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            2597..3124
                     /locus_tag="O0305_001592"
     CDS             2597..3124
                     /locus_tag="O0305_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCY0274739.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            3125..4225
                     /gene="yqeH"
                     /locus_tag="O0305_001593"
     CDS             3125..4225
                     /gene="yqeH"
                     /locus_tag="O0305_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCY0274740.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            4239..5045
                     /gene="aroE"
                     /locus_tag="O0305_001594"
     CDS             4239..5045
                     /gene="aroE"
                     /locus_tag="O0305_001594"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCY0274741.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELEGFNITIPHKERIIPYLDYVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            5049..5339
                     /gene="yhbY"
                     /locus_tag="O0305_001595"
     CDS             5049..5339
                     /gene="yhbY"
                     /locus_tag="O0305_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCY0274742.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            5342..5911
                     /gene="nadD"
                     /locus_tag="O0305_001596"
     CDS             5342..5911
                     /gene="nadD"
                     /locus_tag="O0305_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCY0274743.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            5901..6485
                     /gene="yqeK"
                     /locus_tag="O0305_001597"
     CDS             5901..6485
                     /gene="yqeK"
                     /locus_tag="O0305_001597"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCY0274744.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            6486..6839
                     /gene="rsfS"
                     /locus_tag="O0305_001598"
     CDS             6486..6839
                     /gene="rsfS"
                     /locus_tag="O0305_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCY0274745.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            6842..7558
                     /locus_tag="O0305_001599"
     CDS             6842..7558
                     /locus_tag="O0305_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0274746.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            7598..8284
                     /locus_tag="O0305_001600"
     CDS             7598..8284
                     /locus_tag="O0305_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCY0274747.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            8376..8837
                     /locus_tag="O0305_001601"
     CDS             8376..8837
                     /locus_tag="O0305_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCY0274748.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            8842..>10663
                     /locus_tag="O0305_001602"
     CDS             8842..>10663
                     /locus_tag="O0305_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HCY0274749.1"
                     /translation="MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIVIYHLLAVSGSHIAAI
                     VFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKI
                     KSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSR"
BASE COUNT         3893 a         1316 c         1939 g         3515 t
ORIGIN      
        1 aaaaatatat ttgttgagag tggtgcatga caaatgtact gctctttttg ttgtgtatca
       61 gtattgtgat gttttaatga gaatattata tgaatcatta tgaaatttaa taaaaataaa
      121 agaaatgatt atcatttttt cttatatact gttaaacggt ttggaatttt taggtataca
      181 ctgtattggt tgatataact caactaataa ttgcgaacag agtatttcaa attgaaaagt
      241 attatgagcg tgatacataa tcaaaattgt aggctcaaga accactacat aataaaccat
      301 aagcggttct ttatcattta tgtctcgctc tcaaatgtaa attaataatt gttttggggg
      361 agtttgaagt taaatattta acaggattta ttttaatatt attgttagaa ggaattttta
      421 caaattcagc gagtgcaatc gaatattcag acttacatca taaaagtaag tttgattcaa
      481 agcgtctaag taatgctaag atgtcattca tcaatccaac tcagcttgaa aataaaaaca
      541 caaacgatag actgttgaag catgatttgt tatttcatga catgttcgta aatgatgatt
      601 ggaaaaagga ttttaaagtt gaatttgaaa atgaggcact ttcaaagaaa tttataaata
      661 aggatatcga tatatttgct ggaaattatg gatacggatg tcatggggga gcaaccaata
      721 aaacgcaatg tagttatggt ggtgttactt taagtgacaa taataaatac gatgattata
      781 agaatatacc ttgtaattta tggattgacg gacatcaaac agaaatagaa ctaactgcag
      841 taaaaacgaa aaagaaaatt gttactattc aagaattaga ggttcaatta agaaattatt
      901 tgaatgagaa gtataagttg tacgaacaag gtggcgacat tgttaaaggg tatgttaaat
      961 attataatga tgatgaacaa aatgtagaat atgattttta taatttaaat ggtgagtatg
     1021 gtcgtgaggt attaaaaatg tatgctgata ataaaactat caatagtgac aaattgcatt
     1081 tagatatcta tttattcaaa tcataattta attaagttaa tcattaataa tctttgacag
     1141 tgaaatataa agtacctgtc attatgttgt tacaaataaa aacagcagta aggatgctaa
     1201 cacttagaaa agtcttactg ctgttttcta ttagaattat ctttaatgca caaaggaatt
     1261 cgatacgaaa caaatatgat taactgtgta attacaaaat aaaactacta tagtaaaaag
     1321 aagataaaga cgatgataat aaatgcatcg aatataattt tcttgatgtt tttatcaaca
     1381 acatcaggta aggacataag tataaatcct agaattagag tgtagcgaca tatgttcata
     1441 attgttgctg taaactcgat attaaatgaa aactttaaaa atatacttat aaataaaatg
     1501 acaaacgacg caattgcaat aaaactgccg tatgtagcta tctttgttac gagactattt
     1561 tgattatttt cagccattgt aaaatcctct tatttcatca ttattatttt aaacatatca
     1621 ttttcctgtt atttaataaa gtttcttgac acgattggtt gatgtaattg atatgattag
     1681 tttttgaaaa atagacaaat tagaagcatc gaattcactg ttatgagttc aaaaataatt
     1741 atttatgata ccaagttatt gaaagagctt tttataaaaa taaagcaaaa gttttataca
     1801 gtaatgaaaa tcatgatact tgtcgttgta gtgattaaaa tgtgataaaa taacaaatgc
     1861 taggattttt taaaagaggt gaatcaaaag atgattggta taattggtgc catggaagaa
     1921 gaagtaacaa tattaaaaaa taaattaaca caattaagcg aaatttcagt tgcacatgtt
     1981 aaattttata ctggcatttt aaaagataga gaagtagtga ttacccaaag tggcattgga
     2041 aaagttaatg ctgcaatttc tacgacatta ttaattaata agtttaaacc ggacgtcatt
     2101 attaatacag gttctgctgg agctttagat gaaagtttaa atgtaggtga cgttcttata
     2161 agtgatgatg taaaatatca tgatgcagac gcaacagcat ttggttatga atatggacaa
     2221 ataccacaga tgccggtagc atttcaatca agtaaacctt taatagaaaa agtatctcaa
     2281 gttgtacaac aacaacaatt aacagctaaa gtaggcttaa ttgtaagtgg tgatagcttt
     2341 atcggtagtg ttgaacaacg ccaaaaaatt aaaaaagcat ttccaaatgc gatggcggtt
     2401 gaaatggaag caactgcaat tgcacaaaca tgttatcaat ttaatgtacc atttgttgta
     2461 gttcgtgcag tttcagactt agcaaatgga gaagcggaaa tgagcttcga agcattttta
     2521 gaaaaagcag ctgtatcatc aagtcaaact gttgaagcat tagtgtctca attataaaaa
     2581 ggtaaaggtg agttaaatgg gtttagttcg caagtttttt atgccgaatt catatgttca
     2641 atcaatattt caaattgatt tagacaagtt agtggacaaa ggcgttaaag gtattattac
     2701 agatttagat aatacgctag taggttggga tgttaaagaa cctacagaac gtgttaaagc
     2761 atggtttaag gaagctaatg aaaaaggaat cactattaca atcgtgtcta ataataatga
     2821 gtctcgtgtt gctagtttta gtcagcattt agacatcgat tttattttta aagcgagaaa
     2881 gccaatgggg aaagcgtttg ataaagcaat aactaagatg aatatcagac cagatcaaac
     2941 tgttgttata ggtgaccaaa tgcttactga tgtatttggt ggtaatcgtc gaggtctata
     3001 tacaattatg gttgttccag ttaaacgaac tgatggcttt attactaagt ttaatagatt
     3061 aattgaaagg cgattattac gtcatttcag taaaaaaggt tatatcacat gggaggaaaa
     3121 ttgattgtct gacattttaa aatgtatcgg ttgtggtgcg ccacttcaat ctgaagataa
     3181 aaataaacct ggttttgtac cagagcataa tatgtttcgt gatgacgtga tttgcagacg
     3241 ttgtttccgc ttgaaaaatt ataacgaagt tcaagatgta ggattagaaa gtgaagactt
     3301 tttaaaatta ttatcaggac ttgcggataa aaagggtatt gtcgtcaatg tcgtggatgt
     3361 atttgacttt gaaggatcat ttattaatgc agttaaacgt attgtcggaa acaaaaaaat
     3421 cattttagca gcaaataaat tggatttatt gccgaaacaa attaataaac gtcgagttaa
     3481 agaatggtta aaacgaacag caagaaaata tggtttggaa gctgacgatg tcgtattaat
     3541 ttcagctgaa aaaggctggg gcatagacga cttattatca tcaattgcga atattcgaga
     3601 aaatgaagat gtgtatattg tagggacaac gaatgttggg aaatctacat tgattaataa
     3661 actgattgaa gctagtgttg gtgaaaaaga tgtagtaaca acttcaagat tccctggaac
     3721 aactttagat atgatagata ttcctttaga tgaaacatca tttatgtatg atacaccagg
     3781 tattattcaa gatcaccaaa tgacgcattt agttagtgaa aaagaattga aaattattat
     3841 gcctaagaaa gaaataaaac aacgcgtata tcaattaaat gaggcgcaga cattattctt
     3901 cggcggtcta gcgcgcatag attatgtatc aggtggtaaa cgtccgttag tttgtttctt
     3961 ttctaatgac ttgaatatac atcgtactaa aacggagaag gctaatgatt tatggcgtaa
     4021 tcaacttggc gatttattaa cgccacctgg aaatccacaa aattttgatc ttaatgaggt
     4081 aaaggctgtt agacttgaaa caggcaaaga gaaacgcgat gttatgatct ctggtctagg
     4141 ctttataact ataggaccag gggctaaagt aatcgttcgt gttcctaaaa atgttgatgt
     4201 tgtattaaga aattctattt tataaggtga ttaaaaaaat gaaatttgca gttataggaa
     4261 atcctatttc acattccttg tcgcccgtta tgcatagagc aaattttaat tctttaggat
     4321 tagatgatac ttatgaagct ttaaatattc caattgaaga ttttcattta attaaagaaa
     4381 ttatttcgaa aaaagaatta gaaggcttta atatcacaat tcctcataaa gaacgtatca
     4441 taccgtattt agattatgtt gatgaacaag cgattaatgc aggtgcagtt aacactgttt
     4501 tgataaaaga tggcaagtgg atagggtata atacagatgg tattggttat gttaaaggat
     4561 tgcacagcgt ttatccagat ttagaaaatg catacatttt aattttgggc gcaggtggtg
     4621 caagtaaagg tattgcttat gaattagcaa aatttgtaaa gcccaaatta actgttgcga
     4681 atagaacgat ggctcgtttt gaatcttgga atttaaatat aaaccaaatt tcattagcag
     4741 atgctgaaaa gtatttagct gaattcgata tcgttattaa tacaacacca gcgggtatgg
     4801 ctggaaataa cgaaagtatt attaatttaa agcatctttc tcccaatact ttaatgagtg
     4861 atattgttta tataccgtat aaaacaccta ttttagagga agcagagcgc aagggaaacc
     4921 atatttataa tggcttagat atgtttgttt accagggtgc ggaaagcttt aaaatttgga
     4981 ctaataaaga tgctgatatt aattctatga aaacagcagt tttacaacaa ttaaaaggag
     5041 aataacatat gcttactggc aaacaaaaaa gatacttaag aagtttagca cacaatattg
     5101 atccgatttt tcaaattgga aaaggcggta tcaacgaaaa tatgattaaa caaatagatg
     5161 atacgttaga aaacagagaa ttgattaaag tacatgtact acaaaataac tttgatgata
     5221 aaaaagaatt agctgaaaca ttaagcgaag ctactcatag tgaattagtg caagtgattg
     5281 gatctatgat agtgatttat agagaatcta aagataataa agaaattgaa ttgccataat
     5341 aatgaaaaag atagtacttt acggcggtca gtttaaccct atccatactg cacatatgat
     5401 agtagctagc gaagtatttc atgaattaca gccagatgaa ttttattttt tacctagttt
     5461 tatgtctcca ttgaaaaagc accatgattt tatagacgtt cagcacagat taacaatgat
     5521 acagatgatt atcgacgagc ttggttttgg agatatttgt gacgatgaaa ttaaacgtgg
     5581 tggtcaaagt tatacctatg acacgatcaa ggcattcaag gagcaacaca aagacagtga
     5641 gttgtacttt gttattggga cggatcagta taaccaacta gagaaatggt atcaaattga
     5701 atacttaaaa gaaatggtta cttttgtagt tgtaaatcga gacaaaaata gtcaaaatgt
     5761 tgaaaatgct atgattgcaa ttcagatacc tagggtagat ataagttcga caatgattcg
     5821 acaaagagtt agtgaaggga aatctatcca agttcttgtt cctaaatccg ttgaaaacta
     5881 tattaagggg gaaggattat atgaacattg aaaaagcaaa acggcttgca aaagagaaac
     5941 tgccagagaa acgttataat cattctttaa gagttgcaga aacagcgatt aaattggcag
     6001 aaatttatga tggagacact agtaaagtag aattagcagg tgtattacat gatttctgta
     6061 aatatgatga tttaggtaaa atgtatcaaa ttgttcgaca atacgaatta ggtaatgatc
     6121 tactgagtta tggtagtgaa atattgcatg gccctgtgtg tgcagcaatc atggaacatg
     6181 aatatggtat caatgatgag gaagtattaa tggctatcaa ataccatact actggacgtc
     6241 aacaaatgac aaaaactgaa aaactgattt ttattgcaga ttacatcgaa cctggaagaa
     6301 caatcccagg agttgatgat attcgagata tggcatacaa tcaaggtagt ttagataaga
     6361 caatttatga aatttctaaa cgcacagtac tatttttaat acagaaagat attacggtat
     6421 ataataagac gattgactgt ttaaattatt ataactatag tgacgaaaga ataaaggatg
     6481 attaaatgaa ttcacaagaa ttattagcaa ttgctgtgga tgcaattgac aataaaaaag
     6541 gcgaagatac gatttcttta gaaatgaaag gtatcagcga tatgacagat tattttgttg
     6601 taacgcacgg aaataatgaa cgacaagttc aagcgattgc tagagcggtg aaagaagtag
     6661 ccaatgaaca aaatatagaa gtaaaacgta tggaaggata caatgaagcg cgttggatat
     6721 taattgactt agctgatgtt gtggtacatg ttttccataa agacgaaaga aattattata
     6781 atattgaaaa gttatatcaa gatgcaccat tagaatcata tagtcaggtt gcgtattaat
     6841 tatgtcgcaa tatgcagaaa tgagcctagt gtacgatcaa ttgactcaag atcaaccata
     6901 tgaaaaatgg tttgaaattg taaaaaatca ctgcaaagat gaatcaaata ttttagatat
     6961 tggatgcggt actggtagtt taacagttca attagaagct ttaggtaatg ttactggtat
     7021 ggatttaagt gttgatatgt tgactgttgc agctcaaaaa tcagcaaatg taaattggct
     7081 tgaaggagat atgacatctt ttgatttgca acaacaattt gatatcatca ctattttttg
     7141 tgactctcta aattatttgc aagatgaaac tgcagtgatt gaaacattca tcaatgttta
     7201 tcatcatctg actgattcag gtgtatttat ttttgatgta catactgttt ataaaatgat
     7261 gactttgttt aataatcaaa gttatattga tgataaaggg gacatttttt tagcatggga
     7321 tgctgtgcaa ggagatttac ctttaagtgt ttatcatgat atgacatttt tcattcgaca
     7381 tgaagatgaa acatattcaa gatttgatga gtctcatttt cagagaacat ttgatgaaaa
     7441 gacatatttg tcttggttag ctcaagttgg ttttaaacat gttgaaacat ttactgattt
     7501 taatatagat gaacataatg aagatgcaga aagattgttt ttcattgcga aaaaataaaa
     7561 gtaaaaccac tcgctcagac gtgtataaag ttgaggagtg gttttattgt atcaattttt
     7621 attacgctat aaagattttt taactcaatg gaagttatat attctaagtg ctgttgtttt
     7681 aattatggta ttaattggtt ttatattctg gagacaagat gattatactt caagaaattt
     7741 tgaaaataaa gatactgctc tgaaacaaag cactagtgaa aatagtagtt tgtccaaagt
     7801 agaagatgtc caggtcaaag atggagataa ttccaaaaat aagggtcctg tatatgtcga
     7861 tgtaaaaggt gctgttaaac atcctaatgt ttataaaatg acatctaagg atagagtagt
     7921 tgatttactt gataaagcac aattattgga ggatgcagat gtaagtcaaa ttaatttgtc
     7981 tgaaaaatta acagatcaaa aaatgatttt catacctcat aaaggacaaa agaatgttga
     8041 accacaaatt gaagtaaaca gtgtgcacga aaaaaatggg aacacaaata atactaaagt
     8101 aaatttaaat acggcatctg tatcagaatt gatgtctgtt cctggagttg ggcaagctaa
     8161 agctaatgca attgttgaat atcgcaacca acaaggtgca tttcaagaaa ttgacgattt
     8221 gaaaaaagta aaaggttttg gaagtaaaac ttttgataaa ctgaaatctt atttcacgac
     8281 ataatggatt attttaattg caaattttat tttttgtgat taatcttata ttattagaaa
     8341 atgttactaa attttaagta tggaaggtga acatcttgga aagaatcaaa tgggaagaat
     8401 attttatggc acaaagtcat ttgctagcat tacgttcaac ttgtcaaaga ttatctgtag
     8461 gtgcaacgat tgttaaggat aatcgtatta ttgctggtgg ttataatggc tctgtagctg
     8521 gcgaggtgca ttgtatagat gaaggatgtt taattgaaga tggacattgt atcagaacga
     8581 tacatgcaga aatgaatgct ttattacaat gtgcaaaaca aggtgtatct actgaaggtg
     8641 caacaatcta tgttactcat tttccatgcc taaattgtac aaagtcaatt attcaagcag
     8701 gtataaagcg tatctactat gcagaagatt atcataacca tgaatatgca actaaattac
     8761 tcaaacaatc tggtattgaa tttaaaaaaa ttccattttc accagaatat gttgctaaat
     8821 atctgactaa aggttaatgt tttgctgttt gtcgcgttat caatgattgt aggagtgctt
     8881 tggaattcta gcaaagtgct ctctacattt cttttcattt tacttttgta tattacttat
     8941 cgtaaaaata aaatcgttta tgcccctatt tctctctttt taatcatttt ctccgcatgg
     9001 tatttacatt attcacaaca agcaatattt aattatatca attatattga acgtaattct
     9061 cagtttaatg agcgtgctca agtaatccaa attcaacgtc aaggtagtga cacatataaa
     9121 ggtaggttga gtttaaaaaa tgaaatatat cctttctttt taacagataa aaagaatttt
     9181 gatttaaaga aaattgaaag tcgtaattgt attgttaaag gacaattcaa agttaatgac
     9241 aataagtttg taactcttaa attacaaagt atagttgtac aaagctgcct agaatcgaac
     9301 cggtctaatt taattgagaa acataaacag tttataatga atcgaattta tgattcgggt
     9361 attaagtttc cggatcgtat tatggcattg attactggtg acgtaaaaga agttaatgag
     9421 caatttaagg aacgtgttaa agagatagtt atatatcatt tgctggcagt tagtggctcg
     9481 catatagctg caattgtatt tttaatttac caacctttaa aacgattaaa tttaccttta
     9541 tttgtcatta aaggaattac aatcattgta ttagctttat ttgctcaata cacaaattat
     9601 gcacctagtg ctgtaagagc tataataatg acaactcttg tactggttat tactaagcaa
     9661 attaaaataa agagtattca gctattagca tttgcattta taattatgtt tattttaaat
     9721 ccactagttg tttatgatat tggatttcaa ttttcattca tcatttcatt ttttattatg
     9781 ctactttttc cttttttaca gcaattgtca aagttacaat cattattcat aattacgttt
     9841 attgcacaat tagcttcatt tatcgttgcc attccaagct ttcatcaact tcaatgggtg
     9901 ggatttttat ctaatttgat ttttgtaccg tactattcga ttatattgtt tccgctatct
     9961 attttattct ttattacaag tcattttatt gtgggattaa cgccgctaaa ttacttggtt
    10021 gacctaagtt ttaattttca tgactggtta ctagacctat tcacaagaat caagcaatca
    10081 catttttctg ttcccaagtt taatgattgg atatttatag tatttataat ttctgtttat
    10141 tacatatttt ggttattggc taaacgtaaa tatatattgg ttacgttttg gactataatt
    10201 attctgacat tattaataac gtttccaaca aattcacatc acaaaattac aatgttaaat
    10261 gtggggcagg gagacagtat tttatatgaa ggcggtaaga accaaaatgt cttgattgat
    10321 acaggtggga aagtgattga tgatactaaa caacctagtt attcaatttc taaatatcat
    10381 attttaccaa cgctaaatga aagagggata aatgaattag agtatctaat tttaacacat
    10441 ccacacaatg accatattgg tgaagtggaa tatattatta gtcatattaa aattaaacat
    10501 atagtgatat acaataaggg atatagtagt aatacattga tgttattatc gaaattaagc
    10561 cataagtaca acattaaact tatggatgta agacaagtta gtagttttaa acttggagat
    10621 agtagttttc tattttttga tagttttatt ccaaatagcc gag
//
LOCUS       DAKTIP010000088        10545 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAKTIP010000088 DAKTIP010000000
VERSION     DAKTIP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.088"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..117)
                     /locus_tag="O0305_001603"
     CDS             complement(<1..117)
                     /locus_tag="O0305_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274750.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVR"
     gene            155..616
                     /gene="tsaE"
                     /locus_tag="O0305_001604"
     CDS             155..616
                     /gene="tsaE"
                     /locus_tag="O0305_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCY0274751.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            597..1259
                     /gene="tsaB"
                     /locus_tag="O0305_001605"
     CDS             597..1259
                     /gene="tsaB"
                     /locus_tag="O0305_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCY0274752.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1232..1696
                     /gene="rimI"
                     /locus_tag="O0305_001606"
     CDS             1232..1696
                     /gene="rimI"
                     /locus_tag="O0305_001606"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCY0274753.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1689..2714
                     /gene="tsaD"
                     /locus_tag="O0305_001607"
     CDS             1689..2714
                     /gene="tsaD"
                     /locus_tag="O0305_001607"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCY0274754.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2816..4426)
                     /locus_tag="O0305_001608"
     CDS             complement(2816..4426)
                     /locus_tag="O0305_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCY0274755.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPAKIYERAKDNVPKI"
     gene            4515..4643
                     /locus_tag="O0305_001609"
     CDS             4515..4643
                     /locus_tag="O0305_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274756.1"
                     /translation="MIKTMSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(4787..6715)
                     /gene="abc-f"
                     /locus_tag="O0305_001610"
     CDS             complement(4787..6715)
                     /gene="abc-f"
                     /locus_tag="O0305_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCY0274757.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDCLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLLLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
     gene            6968..7603
                     /locus_tag="O0305_001611"
     CDS             6968..7603
                     /locus_tag="O0305_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCY0274758.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            7907..8986
                     /locus_tag="O0305_001612"
     CDS             7907..8986
                     /locus_tag="O0305_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCY0274759.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVHQQSAKVKQQTQIV"
     gene            9046..9270
                     /locus_tag="O0305_001613"
     CDS             9046..9270
                     /locus_tag="O0305_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCY0274760.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            9478..>10545
                     /locus_tag="O0305_001614"
     CDS             9478..>10545
                     /locus_tag="O0305_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCY0274761.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILC"
BASE COUNT         3464 a         1493 c         1913 g         3675 t
ORIGIN      
        1 tcttacaatt aactttgaat agataatctg tttcaaagat aagaaattta agttgaaatt
       61 tgttacaata taaaggttaa atataaagtt agatacgatt gaacaatgga taatcatttt
      121 tatgaatgaa aagcataata taggagaatc aacattgata aagataaata atttagatga
      181 aatgaatcaa tttgctatat ttttagttga gcaattgaaa agtggtgatt tgattttact
      241 taacggagat ttaggagcag gtaaaacaac gttaacgcaa tttataggaa aagctcttgg
      301 tgtaagacgt acgattaatt ccccgacatt taacatcatt aaatcatata ggggtaaaaa
      361 tttaaaattg catcatatgg attgttatcg cttagaagat tctgatgaag atttaggatt
      421 tgatgaattt ttcgaagatc aggcaattac tgttattgaa tggagtcaat ttataaaaga
      481 tttacttcca gcgacgcatt tatctattaa catttcaaca atatctgaaa atacaagaca
      541 aattgagttg ttcgcgcaag gagaacatta tgaacaaatt aaggaggcaa ttatccatga
      601 attcgctgct cattgataca tcgaatcaac cattatcagt agcattgatg caaaacgatg
      661 aagttttagc tgaaataact acggattcta aacaaaatca ttcagtgcaa ttaatgcctg
      721 cgattagtca gctatttgag cagagcaaaa tagctaaaca gcaattagat gcgattattg
      781 ttgctgaagg tcctggttca tatactggct tacgtatcgg tgttactgtt gctaaaacat
      841 tagcgtatgc gttagatgtt aaattatatg gtgtttcatc attaaaggca ttggcagcaa
      901 caatcgatca tactgataaa ttattggttc ctgtgtttga tgcaagacga caagcagttt
      961 atacaggcat attccagtgg caaaatggtc aattagtgac tattttagaa gatcaatata
     1021 tgtcgataga ggcattgcga gcttttttag aagatttaaa ccaaccattt atttatattg
     1081 ggaaagatac tgtaaaatta caagatgaat tacaaggtga agtgattgcg cagctaccaa
     1141 atgcatcagt catgtatcaa ttgctagata aaccttcaga cgtacataca tttactccta
     1201 aataccataa attagctgag gcggaacgaa attggatcaa cagtcaaaag aacaattaaa
     1261 cattagagag atgactaaag aagatgtgcc acaagtcttt gatcttgagc gtcggagttt
     1321 caatgacagt tcttggacaa ttgacgcatt ttatcatgaa atagagcaaa ataactttgc
     1381 gaaatatttt gtcttagaat ttgaacaaca aattattggt tatttaggtt tatggattgt
     1441 tatcgatcaa gctcaaatta caacagttgc aatcgatgat aattatagag gctatggttt
     1501 aggtcaaatg cttttaaaat acggaataaa ctatgcaagt cacacttgtg atgtgatgag
     1561 tttagaagta agagttaata ataaagtggc acaacatgta tatgaaaatt taggatttca
     1621 atatggtggt aaacgtaaaa attattatgg tgaaggtgag gacgcaatgg ttatgtgggt
     1681 gaatttaaat gactaaagat atattaatac tagctgttga aacaagttgt gatgaaacaa
     1741 gcgttagtgt tataaaaaat ggcagagata ttttatcaaa tacagtttta agtcagattg
     1801 aaagtcataa acgatttggc ggtgtcgttc ccgaagtggc aagtagacat cacgttgaag
     1861 gtataacagc aacaataaac gaggctctag gggatgccga tgtatcaata gaagatattg
     1921 atgccatagc ggttacagaa ggccctggac taattggtgc gttactaata ggtgttaatg
     1981 cagccaaagc attggcattt gcttacgata agccacttat tcctgttcat catattgcag
     2041 gacatatata tgctaatcac atagaagagc cattaacatt cccgctaatt gcacttattg
     2101 tttcaggtgg acatactgaa ttagtttata tgaaggatca tttatcattt gaagtcattg
     2161 gtgaaacacg agatgacgca gtaggtgagg cttatgataa agtggcacga acaattggtt
     2221 taaattatcc aggtggtcca caagttgatc ggttggctgc tgaaggtgaa gatacttatt
     2281 cattccctcg tgtttggttg gataaagata gttatgattt tagttttagt gggttgaaaa
     2341 gtgccgtaat caatcaactt cacaatcaac gacaaaaaaa tattccaatc attgaagcta
     2401 acgtagcaac gagctttcaa aacagtgttg tagaggtgct cacgtttaaa gctattcaag
     2461 cttgtaaaga atatggtgtt cagcgattaa ttgttgctgg tggcgtggcg agtaataaag
     2521 gattacgtca atctttagcg gatcaatgca aagtcaatga cattcaatta actatcccaa
     2581 gtcctaaatt atgcacagat aatgctgcaa tgataggcgt tgccggccac tatttgtatc
     2641 agcaaggtcg atttgctgat ttagcattaa atgggcacag caatatagat ttagaagagt
     2701 attctgcaga ataaaataaa cgtcagttat taacatgatt actatgaaca ttattgaaac
     2761 tgttcatagt cttctgtcat gtgatagctg acgtttttat gtttaaagtt aaatgctaaa
     2821 ttttcgggac attatctttt gctcgttcat atatttttgc tggaaatgaa gtgattttta
     2881 ataattcgat ggcattacgt gtatttgctt tgccaggctt aattttgtaa tcaaaatgta
     2941 tgttattatt ttctattacc tcattgaaat ggtaattttc ataacgttgt tttaagagtt
     3001 cagctaactc aatatcatgt gttgctgcaa taactctaaa gttagatttt tcatgtaaaa
     3061 atgatagtac tgattctgaa gcggcaattc gttcagttgt gttggtacct ttaaaaattt
     3121 catctataaa gcagtatatt ttttgattat ctggaatttc aacaatacgc ttaatagatt
     3181 ttaattcagc cataaaataa ctatcgcctg ataacacatc atcggcattt gccattgacg
     3241 tgaacacaat tccaggttga tatacaaatt tgcttgcggt aactgtttgt atagccgatg
     3301 caagaataat gtttattgct atggatttca taaatgttga tttacccgaa gcattagacc
     3361 cagttaacag tatattttgt gatagtgaga aatcattggc tactgcatct gctatgagtg
     3421 gatgtgttaa ctcagagaat actatgccat catttgaatc atcaatttgt ggttcagtat
     3481 aacattctag cgttctacga tacattgcta atgcataatg attatctaat tcagctatgt
     3541 aatcgaaaca atttttaagc tcattcatat gtgttgtata gcttttttga attgaatgaa
     3601 ataaaacata atcaagcatg aaaataagtt taactagttt aattaccata gctccgatat
     3661 cttcatcatt aacttcagct aaaacactcg ttaaatgacg tgctgttcta aattgcttaa
     3721 aattgacttc aggttgtgga gcatgtttaa tttttgagat agtatagcct tgttttaaaa
     3781 cattggatgc ataaaagata gattttaagt catcctcata tgtacgcttt aaagttgctg
     3841 ataaaatgat attaaatagg caactcataa gaaataataa aataccaact tgtgaattaa
     3901 taaatataac tgcaaatgag ataactggta aaaacgggca tagaaacaat atattttgac
     3961 gttttaccgg tgtgatttgg tctggaaatg ttggataaac agttttacca atcaaagcta
     4021 aatgatatgt tacgtttttt ctaaattcag cattatcatt aaataaacta agtaatgatt
     4081 tgttcgtaat tgaaagcatt ccacgtaaac aggcatatag cttcatttca ccaatagctg
     4141 ttaaattata attcatctta tgaaagattg cattcatatt taagtcggac caagttttat
     4201 catcaactaa tgaatgtgat tcaaattttt ccttgtagag cttatactca tagtcgaaat
     4261 gtgagttcgg tctgataaaa gcttctaatg gagaaatcgt ttgccataat gtttccatag
     4321 attttaatag acgttgacga ctcatgtatc gtccaacaat tcctattaca gatgtaagta
     4381 gaataattgc tataacaaat attaaaaatg tttgatttgt gctcataagt atccccttat
     4441 ttatttttaa taataataaa aatatacttc aatattatta ttatttcata aagaaaatgt
     4501 cttaacaatg aattttgata aaaacaatgt caatagagaa catgcgtact tatcaacaaa
     4561 gagtgaataa cttgtggata aaaggtgttg ataacgtcat ttattgtgga tatcaggtgg
     4621 gaaaacagaa aataaatgat tgattagtgt taatttactt gtgggtaact aggataagtc
     4681 tgtggatagc tataatatca gcgtttttat tgtgaataaa aaaattaaaa tttgtaaaaa
     4741 aataactcta actatagcga ttttacagtt agagttatgt atatttttat agtttttgtt
     4801 gtaattcttc ccatttagac atagcgtgtt ctaatttttg ttcgctatct tgtttttgga
     4861 tagctaattc gtttgctttt tgtggattgt tatacacttc tggttgggtt aattgctcgt
     4921 ctatttgtaa gatagtagtc tctaaagttt ctatttcatt ttcacactcg gatatttgtc
     4981 gttcaagttt gcgttgttca cgtctttgtt ctttttggtt atgatacgac gattgttcca
     5041 tagacttttt atgtgcttgt acgttaactg actgttcttc ttggtgcgct ttcaacgctt
     5101 ccgcttcctc aactttttca atgtaatatt gataatctcc taaatacatc tttccgccat
     5161 caattgttaa atcaaatacc ttgtttgcta attggttaat aaagtaacga tcatgggaaa
     5221 caaatagtat tgttccttca aaatgttgga gtgcttgctc taacatttct tttgaatcta
     5281 tatcgaggtg gttggtaggt tcatctaaaa taagtacatt gtcacgttgc aacataagta
     5341 gtgctagtag taaacgtgct ttttcaccac cagataaatc attaattatc tttttaacat
     5401 cgtcttgtac aaataagaaa cgtccaagaa ctgctcgaat atctttttca ttcattaacg
     5461 gatattgatc ccacacataa tctaaaatcg ttttactaga tttaaattct gcttgctttt
     5521 gatcataata accaatttgt aaatttgcgc cgaaagtaat atcgccatta agcgcttttt
     5581 gttgattagc aatagtttta attaaggtcg attttccaat accatttggc ccaatgattg
     5641 ctatatgatc gcctttagag acctctatac tcataggttt ggtaattgca gtttgataac
     5701 cgatttctaa attttttaca tgcatgacgt cattacctgt attccggtca aagccaaatt
     5761 gaatatttgc acttttggca tctaacattg gtttatcaat gcgttccatt ttttctaaaa
     5821 tcttacgtct actttttgcc attccacttg ttgaagcacg ggtaatattt ttctcaacaa
     5881 aagtttctaa tcgttttatt tctgcttgtt gactttcata ttcttgcatt cgtttttgat
     5941 aatataaatc ccgttgctgt ataaattcct cgtaattacc aacatagcgt ttgacatcac
     6001 ctaaagccac atcataaatt tgagtaacta ttttatctaa aaagtaacga tcatggctga
     6061 tgatgacgat tgcaccttta aaataacgta gataatcttc aagccacttt gtcgtttcta
     6121 aatctaagtg gttagtaggt tcatctaaaa gtaataaatc aggttcgttt aatagcattt
     6181 gagctaaaga taaacgtgtt ttttggccac cgctaaaatc attgataggt ttattgaaat
     6241 cttcttcact aaaatttaac ccgtaaagta ctgttttaat tttactttca tattgatatc
     6301 cttctaattg ttcaaattga ttcgataaag attcataacg ggacatatgt gttttatatg
     6361 tatcactatc gtaatcattt gcatgttttg ataaacaatc tgtttcttct ttgattaagc
     6421 tttccattcg tttaatatgt tcaaatggtt ttgacatttc ttcaaaaact gttgcattag
     6481 aatttaaagt catttgttga gttaaataac ctagttttaa gtttttaatt tttgacacat
     6541 ttccagaatc atagttttct acgccagcta taattttcat caatgttgat ttaccggcgc
     6601 catttcttcc tacgatacca attcgttcac cagtttttac ttcaaaatca acatcagtaa
     6661 atatatcttc accatcgaac gattttgata tatgattgag ttgtaaaagt atcattcgat
     6721 cttcaccttt cgtctttctc aaattttatt aacttatgaa aaaagtcgta attattacat
     6781 taataaccat tttacagtat aaaacgccgt cttgaaacta agatatttat ttttaaaatt
     6841 caataatgta taatatttag gtattcattg taatcaatta tttgtttttt aataatctaa
     6901 caattacgct ctaactcgtt tgaatacata gatataattc attcaaacac gaaggaggaa
     6961 atagcgaatg agtgaccaag ttaaaattcc tcgagcaact ttaaaacgtt tgccgttata
     7021 ttatagattt gtcagttcat taaaatctaa aggtatagat cgtgtaaatt caaaagcgat
     7081 tagcgatgcg ttacaaattg actcggcaac aattcgtcgt gacttttcat attttggcga
     7141 attaggtaaa aaagggtacg gatataatat agatagttta ttggatttct ttaaatctga
     7201 actaagcgag agtgacatga tcaaaatcgc aattgtcgga gttgggaacc tagggaaagc
     7261 tttgctcaca tataactttt caatacatga cgatatgacg attacagaag cgtttgacgt
     7321 aaaagaagat gttattggcc agaaaatagg gaacgttatt gttaaagata acgatgaatt
     7381 aataacaaca ttgaagaagg aagaaataga tgttgtgatt ctaactacac cagaaagagt
     7441 tgcacagaaa gttgcagatg aactcgtcca agctggtgtg aaaggtattt taaacttcac
     7501 tcctggtaga attaatacgc cttcagatgt gcaagtacat caaattgact taggtataga
     7561 attacagtca ttattattct ttatgaaaaa ttacagtgaa taaatgtgat gttagctttg
     7621 aatgattaac cttgttatat ctttaattaa ttatagaaca gtctgggact aagtatgtct
     7681 caggcttttt ctgttatgtt tgaaaatgaa aggaaaaact gttgtgttga taaatatagt
     7741 ataggttaat aaattaacac gtagagatat aatgatgaat cgtaattaat cgccttcctt
     7801 atatgaatat aatataaata tcaaaaatat tgtgtataag gttgaaaaca agaaaatcat
     7861 cacctataat acatactaaa cttatcggaa atgagaggtt aagattatgt ggatgatttt
     7921 tagtggctta attgtcgggg gactcttagg ttttgtcatg caaagaacaa gattttgttt
     7981 aacaggtggc tttcgagata tgtatgtgca aaagaataat aagatgttct atgcattatt
     8041 aatcgctatt actattcaaa gtataggatt attgattttg acggcaacag atattttaca
     8101 aattcctgca catagttttc caatattggg aacaattata ggttctttta tttttggaat
     8161 tggaatagta ttggctggag gatgtgcaac aggtacttgg tatcgcgctg gtgaagggct
     8221 aattggtagt tggattgcat tagtattata tgctgttact gcagcaatca ctaaaacagg
     8281 gattttaaag ccagtaatgg ataaaattaa tcaaccaacg aatgtaaata gtgatatgtc
     8341 tcaaacaact ggcattccgt tttggggatt agtcgttata ttaactataa tcaccatttt
     8401 tctagttgta agaacactca ataataagaa agttcgagtt gctgttccaa aactaaagca
     8461 acgttataca ggtattagat attacctttt cgaaaaacga taccatccat ttattgcagc
     8521 aattgtaatt ggacttatcg cactcttagc ttggccaatg agtgcatcaa ctggaagaaa
     8581 tgacggttta ggtataacaa cgccttcagc aaatttagta cactttttga ttacaggtga
     8641 aactaaattt attgattggg gtgtcttttt agttctagga attttcattg gttcatatat
     8701 tgcagctaga ggatcaagag aatttaaatg gcgattgcca gacaagatta caatacgaaa
     8761 cagtgccatt ggtggcatat gtatgggatt tggtgcgtca gttgctggtg gttgttctat
     8821 cggtaacggt ttggttgaaa cggcaacgat gacttggcaa ggatggattg cgctagcatc
     8881 gatgatagtt ggtgtatgga caatgagtca ttttatcttt gttcgtccaa tgaaaaaagt
     8941 acaccaacaa tctgcaaagg ttaaacagca aacgcaaata gtatagaaga ttattatgca
     9001 aatgatgttg atcaaataaa agtgattgga aaaggagaaa taattatgat acacgaatta
     9061 ggtacagtag gaatggtatg tccatttccg ttaattgaag cgcaaaagaa aatggcaaca
     9121 ttgcaatctg gagatgaatt aaaaattgat tttgattgca cgcaagcgac ggaagccatt
     9181 ccaaattggg ctgcagaaaa tggttatcct gtaacaaact atgaacaaat tgataatgct
     9241 tcatggacaa ttacaattca aaaagtttaa cgttatcatt ttaacaataa aatagatatt
     9301 agattctatg gctacttccg ctaatttaaa agtgagtaag tagtcttttt tgttttagtt
     9361 catgaaatca tttttatata gtgtggcaca ttttattcca aaagatgtaa taaaacttaa
     9421 cgcatttttg ctttttataa attgtcagat tattatgaaa aaaagggagt ggtaagtatg
     9481 aatcttaacg atacgatatt tatgtttttg tgtacattat tagtttggtt aatgacacca
     9541 ggattaagtt tattttatgg tgggttagtt caatctaaaa atgcgcttaa tactgtcatg
     9601 caaagtatgg cagcaattgt gcttgttaca tttgtatgga taacagttgg ttttacaatt
     9661 agttttggga atgggaattt atggttcgga aattgggaat atacttttct taatcatgta
     9721 ggttttgcga ctcaagaaga tattagccca catattcctt tcgctttgtt tatgttattt
     9781 caaatgatgt tttgtacgat tgcaatttct attttatctg gttcaatcgc tgagaaaatg
     9841 aagtttattc cttatttatt attcgtagta atatggactg ctcttgtata cagtccagta
     9901 gcacattggg tttggggcgg cggttggatt aacaaactcg gtgtattaga tttcgctgga
     9961 ggtacggttg ttcatattac atcaggtgtt tctggtttag tattagctat tatgattgga
    10021 aaaggaaaca aacattctga atcaacacca cataatctta tcattacgtt gattggcggt
    10081 atattcgtgt ggattggttg gtatggattt aatgtaggta gtgcttttac atttgataat
    10141 attgcgatgc ttgcatttac aaatactgtc atttcagcca gtgcaggtgc tataggttgg
    10201 ttaattttag aatatatttt taaaaagacg acaagtttac ttggactttt actcggtgca
    10261 ttagcaggat tagttgtcat tactcctgca gcaggatatg taacatatct tagtgcaaca
    10321 ataatggctt taataggagg tatctgttgt tatattgtca ttaattacat caaggtaaaa
    10381 ctaaaatatc atgatgcatt agatgcattt ggtattcatg gtgttggtgg tattattggt
    10441 gctgttttaa cagcagtttt ccaaagtaaa aaagccaatc ctgacattga gaatggcttt
    10501 atttatactg gtgacataca tattatactt gtacaaatat tatgt
//
LOCUS       DAKTIP010000089        10265 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAKTIP010000089 DAKTIP010000000
VERSION     DAKTIP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.089"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..2614
                     /locus_tag="O0305_001615"
     CDS             <1..2614
                     /locus_tag="O0305_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCY0274762.1"
                     /translation="IVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVKA
                     LIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLIQ
                     LWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKSI
                     FTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWNS
                     IKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLNW
                     VGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEIIE
                     FPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSAF
                     NWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDITK
                     AAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEGV
                     DFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTMV
                     KPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGSG
                     ATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSRG
                     LFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKVF
                     DGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGFD
                     DPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQE
                     STKVKFRKGGIAIQ"
     gene            2611..4095
                     /locus_tag="O0305_001616"
     CDS             2611..4095
                     /locus_tag="O0305_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCY0274763.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYK
                     GRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            4111..7896
                     /locus_tag="O0305_001617"
     CDS             4111..7896
                     /locus_tag="O0305_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274764.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTTETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            7886..8038
                     /locus_tag="O0305_001618"
     CDS             7886..8038
                     /locus_tag="O0305_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274765.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            8085..8372
                     /locus_tag="O0305_001619"
     CDS             8085..8372
                     /locus_tag="O0305_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274766.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            8430..8726
                     /locus_tag="O0305_001620"
     CDS             8430..8726
                     /locus_tag="O0305_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCY0274767.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            8876..9052
                     /locus_tag="O0305_001621"
     CDS             8876..9052
                     /locus_tag="O0305_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCY0274768.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(9105..9212)
                     /locus_tag="O0305_001622"
     CDS             complement(9105..9212)
                     /locus_tag="O0305_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274769.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            9264..9518
                     /locus_tag="O0305_001623"
     CDS             9264..9518
                     /locus_tag="O0305_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCY0274770.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            9530..>10265
                     /locus_tag="O0305_001624"
     CDS             9530..>10265
                     /locus_tag="O0305_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0274771.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERL"
BASE COUNT         3736 a         1431 c         2147 g         2951 t
ORIGIN      
        1 tattgttcaa gcgcttcaaa atatatgcaa ttttatcaaa gttatatttg aatttatctt
       61 aaattttgta attaaaccaa taatgtttgc aatctggcaa gtgatgcaat ttatttggcc
      121 ggcggttaaa gccttgattg tcagtacttg ggagaatata aaaggtgtga tacaaggaac
      181 tttaaatatc atactaggtt taattaagtt cttctcaagt ttatttactg gagattggcg
      241 aggagtttgg gacgcgatta ttatgattct taaaggagtc gttcaattaa tatggaattt
      301 aattcaatta tggtttgtag gcaaaatact tggcgttgtt aggtactttg gcggattgct
      361 aaaaggatta ataacaggta tttgggacgt aataaaaagt atattcagta aatctttatc
      421 agcaatttgg aatgcgacaa aaagtatttt tggattctta tttaatagtg tcaaatcaat
      481 tttcacgaat atgaaaaatt ggttatctaa cacatggagt agtatccgta cgaatacgat
      541 aggaaaagca cagtcattat ttagtggtgt gaaatcaaaa ttcactagtt tgtggaatgc
      601 gacaaaagat atttttagta atctaagaaa ttggatggca aatatttgga attccattaa
      661 agataatacg gttggaattg caagtcgttt atggagtaaa gtgcgtggaa tttttacaaa
      721 tatgcgtgac ggcttacaaa gtattatcag caaaattaaa agtcatatcg gcggtatggt
      781 agatgctatt aaaaaaggac ttaataaatt aattgaaggt ttaaactggg tcggtggtaa
      841 gctgggaatg gataaaatac ctaagttaca cactggtaca gaacacacac atactactac
      901 aagattagtt aagaacggta agattgcgcg ggatacgttc gctacggttg gggataaagg
      961 acgtggaaat ggtccgaatg gtttcagaaa tgaaataatt gaattcccta atggtaaacg
     1021 tgtaatcaca cctaatacag atactaccgc ttatttacct aaaggctcaa aagtatacaa
     1081 cggtacacaa acttattcaa tgttaaacgg aacgcttcca agatttagct taggtactat
     1141 gtggaaagat attaaatccg gtgcatcatc ggcatttaac tggacaaaag atcaaatagg
     1201 taaaggtacc aaatggcttg gcgataaagt tggcgatgtt ttagatttta tggaaaatcc
     1261 tggcaaactt ttaaattata tacttgaagc ttttggaatt gatttcaatt ctttaactaa
     1321 aggtatggga attgcaggcg acataacaaa agctgcatgg tctaagatta agaaaagtgc
     1381 tactgattgg ataaaagaaa atttagaagc tatgggcggt ggcgatttag tcggcggaat
     1441 attagaccct gacaaaatta attatcatta tggacgtacc gcagcttata ctgctgcaac
     1501 tggaagacca tttcatgaag gtgtcgattt tccatttgta tatcaagaag ttagaacgcc
     1561 gatgggtggc agacttacaa gaatgccatt tatgtctggt ggttatggta attatgtaaa
     1621 aattactagt ggagttatcg atatgctatt tgcgcatttg aaaaacttta gcaaatcacc
     1681 acctagtggc acgatggtaa agcccggcga tgttgttggt ttaactggta ataccggatt
     1741 tagtacagga ccacacttac attttgaaat gagaagaaat ggacgtcact ttgaccctga
     1801 accatattta agaaatgcaa agaaaaaagg taggttatca attggtggcg gtggtgctac
     1861 ttctggaagt ggtgcaactt atgcaagccg agtaatccgt caagcgcaaa gtattttagg
     1921 aggacgttat aaaggtaagt ggattcatga ccagatgatg cgagttgcaa agcgtgaaag
     1981 taactatcaa tcaaatgcag tgaataattg ggatattaat gctcaaagag gagacccatc
     2041 aagaggatta ttccaaatca tcggctcaac ttttagagca aacgctaaac gtggatatac
     2101 taactttaat aatccggttc atcaaggtat ttcagcaatg cagtacattg ttagacgcta
     2161 cggttgggat ggatttaagc gtgctggtga ttacgcatat gctacaggtg gaaaagtttt
     2221 tgatggttgg tataacttag gtgaagacgg tcatccagaa tggattattc caacagatcc
     2281 agctcgtaga aatgatgcaa tgaagatgtt gcattatgca gcagcagaag taagagggag
     2341 aaaagcgagt aaaaataagc gtcctagtca attgtctagt gtaaatgggt ttgatgaccc
     2401 aagcttatta ttgaaaatga ttgaacaaca gcaacaacaa atagctttat tactgaaaat
     2461 agcgcaatcc aacgatgtga ttgcagataa agattatcag ccgattattg acgaatacgc
     2521 ttttgataaa aaggtgaacg cgtctataga aaagcgagaa aggcaagaat caacaaaagt
     2581 aaagtttaga aaaggaggaa ttgctattca atgatagaca ctattaaagt gaacaacaaa
     2641 acaattcctt ggttgtatgt cgaaagaggg tttgaaatac cctcttttaa ttatgtttta
     2701 aaaactgaaa atgtagatgg acgttcgggg tctatatata aagggcgtag gcttgaatct
     2761 tatagttttg atataccttt ggtggtacgt aatgactatt tatctcacaa cggctttaaa
     2821 acacatgatg acgtcttgaa tgaattagta aagtttttta actacgagga acaagttaaa
     2881 ttacaattca aatctaaaga ttggtactgg aacgcttatt ttgaaggacc aataaagctg
     2941 cacaaagaat ttgcaatacc tgttaagttc actatcaaag tagtactaac agacccttac
     3001 aaatattcag taacaggata taaaaatact gcgatttcag accaagtttc agttgtaaat
     3061 agtgggactg ctgacactcc tttaattgtt gaagcccgag caattaaacc atctagttac
     3121 tttatgatca ctaaaaatga tgaagattat tttatggttg gtgatgatga ggtaaccaaa
     3181 gaagttaagg attacatgcc tcctgtttat catagtgagt ttcgtgattt caaaggttgg
     3241 actaagatga ttactgaaga tattccaagt aatgatttag gtggtaaagt cggcggtgac
     3301 tttgtgatat ccaatcttgg cgaaggatat aaagcaacta atttttctga tgcaaaaggt
     3361 tgggttggtg ctggcacgaa acgagggctc cctaaagcga tgacagattt tcaaattacc
     3421 tataaatgta ttgttgaaca aaaaggtaaa ggtgccggaa gaacagcaca acatatttat
     3481 gatagtgatg gtaagttact tgcttctatt ggttatgaaa ataaatatca tgatagaaaa
     3541 ataggacata ttgttgttac gttgtataac caaaaaggag accccaaaaa gatatacgac
     3601 tatcagaata aaccgataat gtataacttg gacagaatcg ttgtttatat gcggctcaga
     3661 agagtaggta ataaattttc tattaaaact tggaaatttg atcacattaa agacccagat
     3721 agacgtaaac ctattgatat ggatgagaaa gagtggatag atggcggtaa gttttatcag
     3781 cgtccagctt ctatcatagc tatctatagt gcgaagtata atggttataa gtggatggag
     3841 atgaatggat taggttcatt caatacggag attctaccaa aaccgaaagg cgcaagggat
     3901 gtcattatac aaaaaggtga tttagtaaaa atagatatgc aagcaaaaag tgttgtcatc
     3961 aatgaggaac caatgttgag cgagaaatcg tttggaagta attatttcaa tgttgattct
     4021 gggtacagtg aattaatcat acaacctgaa aacgtctttg atacgacggt taaatggcaa
     4081 gatagatatt tatagaaagg agatgagagt gtgatacatg ttttagattt taacgacaag
     4141 attatagatt tcctttctac tgatgaccct tccttagtta gagcgattca taaacgtaat
     4201 gttaatgaca attcagaaat gcttgaactg ctcatatcat cagaaagagc tgaaaagttc
     4261 cgtgaacgac atcgtgttat tataagggat tcaaacaaac aatggcgtga atttattatt
     4321 aactgggttc aagatacgat ggacggctac acagagatag aatgtatagc gtcttatctt
     4381 gctgatataa caacagctaa accgtatgca ccaggcaaat ttgagaaaaa gacaacttca
     4441 gaagcattga aagatgtgtt gagcgataca ggttgggaag tttctgaaca aaccgaatac
     4501 gatggcttac gtactacgtc atggacttct tatcaaacta gatatgaagt tttaaagcaa
     4561 ttatgtacaa cctataaaat ggtattggat ttttatatag agcttagttc taataccgtc
     4621 aaaggtagat atgtggtact caaaaagaaa aacagcttat tcaaaggtaa agaaattgag
     4681 tatggtaaag atttggttgg gttaactagg aagattgata tgtcagaaat caaaacagca
     4741 ttaattgctg tgggacccga aaatgacaaa ggaaagcgtt tagagctagt tgtgacagat
     4801 gacgaagcgc aaagtcaatt caacctacct atgcgctata tttgggggat atatgaacca
     4861 caatcagatg atcaaaatat gaatgaaaca cgattaagtt ctttagccaa aacagagtta
     4921 aataaacgta agtcggcagt tatgtcatat gagattactt ctactgattt ggaagttacg
     4981 tatccgcacg agattatatc aattggcgat acagtcagag taaaacatag agattttaac
     5041 ccgccattgt atgtagaggc agaagttatt gctgaagaat ataacataat ttcagaaaat
     5101 agcacatata cattcggtca acctaaagag ttcaaagaat cagaattacg agaagagttt
     5161 aacaagcgat taaacctaat acaccaaaaa ttaaacgaca atattagcaa tatcaatact
     5221 atagtaaaag atgttgtaga tggtgaatta gaatactttg aacgcaaaat tcataaaagt
     5281 gatacaccgc cagaaaatcc agtcaatgat acgctttggt atgatacaag taaccctgat
     5341 gttgctgtct tgcgtagata ttggaatggt cgatggattg aagcaacacc aaatgatgtt
     5401 gaaaaattag gtggtataac aagagagaaa gcgctattca gtgaattaaa caatattttt
     5461 attaatttat ctatacaaca cgctagtctt ttgtcagaag ctacagaatt actgaatagc
     5521 gagtacttag tagataatga tttgaaagcg gacttacaag caagtttaga cgctgtgatt
     5581 gatgtttata atcaaattaa aaataattta gaatctatga caaccgaaac tgcaacgatt
     5641 ggtcggttgg tagatacaaa aactttattt cttgagtata gaaagaaatt acaagatgtt
     5701 tatacagatg tagaagatgt caaaatcgcc atttcagata gatttaaatt attacagtca
     5761 caatacactg atgaaaaata taaagaagcg ttggaaataa tagcaacaaa atttggttta
     5821 acggtgaatg aagatttgca gttagtcgga gaacctaatg ttgttaaatc agctattgaa
     5881 gcagctagag aatccacaaa agaacaatta cgtgactatg taaaaacatc ggactataaa
     5941 acagacaaag acggtattgt tgaacgttta gatactgctg aagctgagag aacgacttta
     6001 aaaggtgaaa tcaaagataa agttacgtta aacgaatatc gaaacggatt ggaagaacaa
     6061 aaacaatata ctgatgacca gttaagtgat ttgtccaata atcctgagat taaagcaagt
     6121 attgaacaag caaatcaaga agcgcaagaa gctttaaaat catacattga tgctcaagat
     6181 aatcttaaag agaaggaatc gcaagcgtat gctgatggta aaatttcgga agaagagcaa
     6241 cgcgctatac aagatgctca agctaaactt gaagaggcaa aacaaaacgc agaactaaag
     6301 gctagaaacg ctgaaaagaa agctaatgct tatacagaca acaaggtcaa agaaagcaca
     6361 gatgcacaga ggagaacact gactcgctat ggttctcaaa ttatacaaaa tggtaaggaa
     6421 atcaaattaa gaactactaa agaagagttt aatgcaacca atcgtacact ttcaaatata
     6481 ttaaacgaga ttgtccaaaa cgttacagat ggaacaacaa tcagatatga tgataacgga
     6541 gtggctcaag ctttaaatgt ggggccacgt ggtattagat taaatgctga taaaattgat
     6601 attaacggta atagagaaat aaaccttctt atccaaaata tgcgagataa agtagataaa
     6661 accgatattg tcaacagcct taatttatca agagagggtc ttgatatcaa tgttaataga
     6721 attggaatta aaggcggtaa caataacaga tatgttcaaa tacagaatga ttctattgaa
     6781 ctaggtggta ttgtgcaacg aacttggaaa ggcaaacgat caaccgatga tatattcaca
     6841 cgtcttaaag atggacatct aaggtttaga aataataccg caggcggttc actttatatg
     6901 tcacattttg gtatttcaac atatattgat ggagaaggcg aagacggagg ttcatccggt
     6961 actattcaat ggtgggataa aacttacagt gatagcggta tgaatggcat aacaatcaat
     7021 tcctatggtg gtgtcgttgc actaacgtca gataataatc gggttgttct ggagtcttac
     7081 gcttcatcga atatcaaaag caaacaggca ccggtgtatt tatatccaaa cacagacaaa
     7141 gtgcctggat taaaccgatt tgcattcacg ctgtctaatg cagataatgc ttattcgagt
     7201 gacggttata ttatgtttgg ttctgatgag aactatgatt acggtgcggg tatcaggttt
     7261 tctaaagaaa gaaataaagg tcttgttcaa attgttaatg gacgatatgc aacaggtgga
     7321 gatacaacaa tcgaagcagg gtatggcaaa tttaatatgc tgaaacgacg tgatggtaat
     7381 aggtatattc atatacagag tacagaccta ctgtctgtag gttcagatga tgcaggagat
     7441 aggatagctt ctaactcaat ttatagacgt acttattcgg ccgcagctaa tttgcatatt
     7501 acttctgctg gcacaattgg gcgttcgaca tcagcgcgta aatacaagtt atctatcgaa
     7561 aatcaatata acgatagaga tgaacaactg gaacattcaa aagctattct taacttacct
     7621 attagaacgt ggtttgataa agctgagtct gaaattttag ctagagagct gagagaagat
     7681 agaaaattat cggaagacac ctataaactt gatagatacg taggtttgat tgctgaagag
     7741 gtggagaatt taggattaaa agagtttgtc acgtatgatg acaaaggaga aattgaaggt
     7801 atagcgtatg atcgtctatg gattcatctt atccctgtta tcaaagaaca acaactaaga
     7861 atcaagaaat tggaggagtc aaagaatgca ggataacaaa caaggattac aagctaatcc
     7921 tgaatataca attcattatt tatcacagga aattatgagg ttaacacaag aaaacgcgat
     7981 gttaaaagcg tatatacaag aaaataaaga aaatcaacaa tgtgctgagg aagagtaatc
     8041 tttagcacta tttttataca aaaatttaag gaggtcattt aattatggca aaagaaatta
     8101 tcaacaatac agaaaggttt attttagtac aaatcgacaa agaaggtaca gaacgtgtag
     8161 tatatcaaga tttcacagga agttttacaa cttctgaaat ggttaaccat gctcaagatt
     8221 ttaaatctga agaaaacgct aagaaaattg cggagacgtt aaatttgtta tatcaattaa
     8281 ctaacaaaaa acaacgtgtg aaagtagtta aagaagtagt tgaaagatca gatttatctc
     8341 cagaggtaac agttaacact gaaacagtat gaaaagctat gagttagata ctcataatct
     8401 ttattctttt agaaagcggg tgtactgaat tggggtggtt caaaaaacac gaacatgaat
     8461 ggcgcatcag aaggttagaa gagaatgata aaacaatgct cagcacactc aatgaaatta
     8521 aattaggtca aaaaacccaa gagcaagtta acattaaatt agataaaacc ttagatgcta
     8581 ttcaaaaaga aagagaaata gatgaaaaga ataagaaaga aaatgataag aacatacgtg
     8641 atatgaaaat gtgggtgctt ggtttagttg ggacaatatt tggatcgcta attatagcat
     8701 tattgcgtat gcttatgggc atataagaga ggtgaataaa atgtttaaac taatctttgg
     8761 ttatagtttc tggacatgtt tttggttcgg taaatgtaaa taagttttag tcagtgcttc
     8821 ggtactgact ttttatttat tgttgtaatt atggtaatat gcagaagtga gcaagttgga
     8881 tagatggtgg ctatctgagt ataaggaggt ggtgcctatg gtggcattac tgaaatcttt
     8941 agaaaggaga cgcctaatga ttacaattag taccatgttg cagtttggtt tattccttat
     9001 tgcattgata ggtctagtaa tcaagcttat tgaattaagc aataaaaaat aaccatcgct
     9061 aactttggct ggtttcgatg gttaaatggt tattaattta atctttaatc taaaatagcc
     9121 accgtctttt taacgggctc attagggtaa catgtttgcg catgttgccc tttttctata
     9181 tataaattaa cacaccataa tataaatatc aaatagacgg cttattagtc gtctttttat
     9241 tttgggtaaa aggagataag aatatgatta attggaaaat tagaatgaaa caaaaatcat
     9301 tttgggtagc gatattgtca gctatctttt tatttgctca aaacatcgca aaagctattg
     9361 ggtatgatat ccaagtttat acagagcaat taacagacgg tttaaacgct atattaggat
     9421 ttttagtatt aactggtgtg attcaagacc cgactactaa aggtataggt gatagccacc
     9481 aagctttaga atatgaagaa ccaagaagaa aatactagga ggtaaaataa tgaaaacata
     9541 cagtgaagca agagcaaggt tacgttggta tcaaggtaga tatattgatt ttgacggttg
     9601 gtatggttac caatgtgcag atttagcagt tgattacatt tattggttgt tagaaattag
     9661 aatgtgggga aatgcaaaag atgcaatcaa taacgatttt aaaaacatgg caacagtata
     9721 tgaaaacaca ccatcgtttg ttccacaaat aggtgatgtg gctgtattta ccaaaggaat
     9781 atataaacaa tacggtcata ttggtttagt gtttaatggt ggtaatacaa accaattttt
     9841 aattttggaa cagaactatg acggtaacgc aaatacgcct gcaaagttac gttgggataa
     9901 ttattacggc tgtactcact ttattagacc taagtataaa agtgagggct taatgaataa
     9961 gatcacaaat aaagttaaac cacctgctca aaaagcagtc ggtaaatctg caagtaaaat
    10021 aacagttgga agtaaagcgc cttataacct taaatggtca aaaggtgctt attttaatgc
    10081 gaaaatcgac ggcttaggtg ctacttcagc cactagatac ggtgataatc gtactaacta
    10141 tagattcgat gttggacagg ctgtatacgc gcctggaaca ttaatatatg tgtttgaaat
    10201 tatagatggt tggtgtcgca tttattggaa caatcataat gagtggatat ggcatgagag
    10261 attga
//
LOCUS       DAKTIP010000090        10220 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAKTIP010000090 DAKTIP010000000
VERSION     DAKTIP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.090"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(453..1697)
                     /locus_tag="O0305_001625"
     CDS             complement(453..1697)
                     /locus_tag="O0305_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCY0274772.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVDEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(1806..2996)
                     /locus_tag="O0305_001626"
     CDS             complement(1806..2996)
                     /locus_tag="O0305_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCY0274773.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            complement(3304..4431)
                     /locus_tag="O0305_001627"
     CDS             complement(3304..4431)
                     /locus_tag="O0305_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCY0274774.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(4793..5170)
                     /locus_tag="O0305_001628"
     CDS             complement(4793..5170)
                     /locus_tag="O0305_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCY0274775.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(5586..6179)
                     /locus_tag="O0305_001629"
     CDS             complement(5586..6179)
                     /locus_tag="O0305_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCY0274776.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            6243..6626
                     /locus_tag="O0305_001630"
     CDS             6243..6626
                     /locus_tag="O0305_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCY0274777.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHDRKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            6757..9162
                     /gene="mnhA1"
                     /locus_tag="O0305_001631"
     CDS             6757..9162
                     /gene="mnhA1"
                     /locus_tag="O0305_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCY0274778.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            9155..9583
                     /gene="mnhB1"
                     /locus_tag="O0305_001632"
     CDS             9155..9583
                     /gene="mnhB1"
                     /locus_tag="O0305_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCY0274779.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            9583..9924
                     /gene="mnhC1"
                     /locus_tag="O0305_001633"
     CDS             9583..9924
                     /gene="mnhC1"
                     /locus_tag="O0305_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCY0274780.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            9917..>10220
                     /locus_tag="O0305_001634"
     CDS             9917..>10220
                     /locus_tag="O0305_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="subunit D of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; contains an oxidoreductase domain; catalyzes the
                     transfer of electrons from NADH to ubiquinone; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HCY0274781.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQT"
BASE COUNT         3106 a         1908 c         1569 g         3637 t
ORIGIN      
        1 aaacagcagt aagatatttc caaattgaaa ttatcctact gctgtctttt tgggagtggg
       61 acagaaatga tattttcgca aaatttattt cgtcgtccca ccccaacttg cacactattg
      121 taagctgact tttcgccagc ttctgtgttg gggccccgcc aacttgcatt gtctgtagaa
      181 attgaggagc taatttctct gtgtcggggc tccaccccaa cttgcacact attgtaagct
      241 gactttccgc cagcctctgt gttggggccc cgccaacttg cacactattg taagctgact
      301 ttccaccagc ctctgtgttg gggccccgac tatttttgaa aagagcgtgt tacacgggca
      361 ttgttttaca gtcaactact gctaaaataa aattaacgag cttagggctt tgttttctgt
      421 cccaagctcg ttaaatcaca tatgataatt aattatgccc aaccacgata tctagctgct
      481 tctgctgtac gtttaatacc tatgatatat gctgcaagtc tcatatctat ttttcggttt
      541 tgagacaatt cgtaaatcgt atcaaatgcc gcttctaatt tttcacgtag cttttcatca
      601 acttcttctt cagaccaata ataaccttga ttattttgta cccattcgaa gtaagaaacc
      661 gttacaccac cagcacttgc taatacgtct ggaactaata atataccacg ttcagttaaa
      721 atacgtgttg cttctggtgt tgtaggtcca ttagcagctt caacaacgat actagcttta
      781 atatcatgtg cattgtcttc tgtaatttgg tttgaaatag ccgctggtac taaaatgtca
      841 caatctaatt caaacaattc tttatttgag attgtttctt caaataaatt tgttaccgta
      901 ccaaaactat cacgacggtc taataaataa tctatatcta agccatttgg atcgtgtaat
      961 gcaccgtaag catcagagat acctacaatt tttgcaccta aatcatataa gaatttagct
     1021 aagaaacttc cggcattacc gaaaccttga ataacaacct tggcaccttc aatttgcata
     1081 ttacgacgtt ttgcagcttg ttcaattgca ataactacac ctagtgcagt tgatctgtcg
     1141 cgtccatgag aaccacccaa tacaattggt ttacctgtga tgaaacctgg tgaattaaat
     1201 ttatctaatg cactatattc atccatcatc caagccataa tttgtgagtt tgtaaataca
     1261 tctggtgctg gaatatcttt gttcggacct acgaattgtg aaattgctct tacatatccg
     1321 cgtgataaac gttcaacttc atgaatgctc atttgacgtg gatcacaaac gataccaccc
     1381 ttaccaccac cgtatggtaa gtttacaatg ccacatttca aagtcatcca cattgataat
     1441 gcttttactt cttcttcatc aacatctggg tggaaacgca cgcccccttt tgttggtcca
     1501 acagcatcat tatgttgcgc acggtaacct gtgaatgttt ttactgtgcc atcatccatt
     1561 cgtacaggga tacgcacttg taacattctt aaaggttctt taattaaatc gtacattcct
     1621 tcgtcaaatc ccaatttatg caatgcttct ttaataattc cttgagtaga agttactaaa
     1681 ttattgttct cagtcatgat ccttttcgcc tcttctttac ctaatgattt cgctttcaaa
     1741 catattgtaa cataacgtat tcctttttaa agcccttaca aactgattgt tacaactttt
     1801 tgacattatt gaaatacatg tcttattttt tcaagtgcaa ggtccaattc ttctttagta
     1861 ataattaatg gtggtgcaaa acgaatgaca gtatcatgcg tttctttaca taataagcct
     1921 tcttctttca aagcttcaca atatggtcta gcactttcat taagttccac acctataaac
     1981 aaaccacgtc cacggacttc tttaattgat ggatgatcaa tttgctttaa ttgttcttta
     2041 aaataatctc ctaattctaa agagcggcct ggtaaatcct catcaacgat aacatctaat
     2101 gcagcaattg atgcagcaca agcaagtgga ttaccaccaa atgttgaacc atgtgagcca
     2161 ggtgtaaaga catctaatac ttctttatct gctaatacaa cagaaattgg gaagactcca
     2221 ccacctagtg ctttacctaa aatatagaca tcaggtttta cattatccca atccgtagca
     2281 aataatttac ccgaacgacc taatcctgct tggatttcgt cagcaataaa taagacatta
     2341 tgttcatcac ataattctct aattgctttc aaatatcctt ctggcggtat atttataccc
     2401 gcttcacctt gaattggttc tactaaaact gctgcagtat tttcattaat tgcagctttc
     2461 aatgcatcta catctccaaa atcaactttt ctaaatccat ctaataacgg accataacca
     2521 cgttggtatt ctgcttctga agataatgaa actggcgcca ttgttcgacc atggaagtta
     2581 ccattaaatg caatgatttc tgctttattt ggctcaattc ctttaacatc gtatgcccag
     2641 cgtcgtgctg ctttcaaagc tgtttctact gcttcagcac ctgtattcat tggtaaagct
     2701 ttatctttac ctgccagttt acaaattttt tcgtaccatt cacctaagtt atcactatga
     2761 aaagcacgtg aaactaaagt cactttatca gcttgatctt ttaatgcttg aataattttc
     2821 ggatgtctat gaccttggtt aacagcggaa tatgcagata acatatccat atatttattg
     2881 ccttcaggat ctttaaccca taccccttca gcttctgaaa tgacaattgg caatggtaaa
     2941 taattatgtg ctccgtaatg atttgttaac tcaataattt tttcagattt agtcatcata
     3001 tctccccttt tcatcattta taactattat acatgaaaca ttatccaaat aattacatta
     3061 gttttcaaag cagatacttt tccaccaaaa aagatgaaat aatcactaag tttcattaaa
     3121 tttgtctatt ttgaaaaccc ttacatttat aatgacataa ttacttaaat gattacaagc
     3181 aaaagaattg ataattttac acttaatcaa aagtatattt tactaagaat atttttattt
     3241 ataaatattg aaaaccacta acaaattgca tacacaatat cattagtggt aacagttaaa
     3301 cacttattta tctttacgtg gtaatggata aaacccttca ttaaattgat gccataaatt
     3361 aggagttaag tgatgattat cttctctctc tggatcaaag taattaataa tttcatcttc
     3421 ccgtccatct ttaatttttt caacaaattg ataatccagt agtagctctc taccaatggc
     3481 tactaagtca acaccaacat tttcaactgc atctaaagct tcgtcagctg tgaaaattga
     3541 accaataccg ataagtggca tacgaccatt tatccatttg tgaattaaag gcagtctttc
     3601 ttgtccagcg tatttacctt cacgtgtcgt tgcatgcgta tccatcattg aaacatgaat
     3661 atagtcgatt ggcatatggc taattttatt aacgagttcc tctgtaattt ccattgtgat
     3721 tcctggagac tccgcttcct ctggagataa tctgtatcca actataaagt ctttattgcc
     3781 atacgcttct ttcgctttaa gtacttcttc aatcacagcg accgggaatt tatattgatt
     3841 tgcccataca tcatttcttc tattatagta tggtgataca aattgatgaa ttaagtaatg
     3901 attcgcgcca tgtatttcaa caccatcaaa ccctgcttca attgcacgtc gcgttgcttc
     3961 accaaaatcc ttgattgctt gttcaatctc ttcattcgtc atttctctag cactatgttc
     4021 ttgtttctga ccaaaacttt ttaaagaaat tggacttggt gctacgacgt ctccatcagg
     4081 tgttaattca ggcaatgctt gtgcaccgcc atgatgtatt tgtacgagtg ctttggcacc
     4141 gtttttcttc attgctgtag ctaatcgttt tagtccttca atattactgt catgcgcgat
     4201 tgatggctgt cctggaaatg cttttccgac atcactcaca ttactcgcag cattaattgt
     4261 aataccaaca tcttgtgaac gcttttcaat ataaggaagt tctacatctg aaatagtacc
     4321 atcatcattt gaagaaatat gtgttaaagg ggctaacaca aatcgatttc tcaactctac
     4381 tccatttggt aattctactt tatcaaacaa tggttcgtat ttacttttca ttacttaatt
     4441 cctccttaaa attattgagt tgactataac atgtaatttt ttcattctcc aattatctgt
     4501 ttatatagtt gcaattcgtt taatacttta agttgtagtt ctcatttcta aaataaataa
     4561 aaaaaacaaa tccacataca atattgcaaa tcaacgattc tatctaacaa agataatgga
     4621 tatgtcacct taaaccattt gtacgtgtaa atagaatcaa tcatattacg ttatcgtaga
     4681 caaaaagaga ttagtatcta ataaaattta atagatacta atctctttta tagtctaaga
     4741 aactttcatt tcagaccctt tgtatgattt tcggtacgat ttatctgttc ctttagtcgt
     4801 ttcgaattgc tcgctttgac tgttttatcc agattggtaa gcgttcttta agtgtttgaa
     4861 acccatattt ttctgtttct ctgatttcat ctaatactga ttgtttttcc ttcttacgct
     4921 cataattttt gaagtaatca ttatccttta atgatagatt aagctttcct tcatcatcta
     4981 tagacaaaat tttagcttta acaatttggc cttctgatag aaatttcttc aaattatgaa
     5041 cgtagtcatc cataatttct gatatatgaa tcagtccttc agtatgatta ggggtctcaa
     5101 caaacgcacc gtatggttga ataccagtta cacgcacctt gatatgttgg ccaattttgt
     5161 agttattcaa cttaataacc ccttatttcg tttacttaac actattatca tagcacaaat
     5221 cgaaatggtg gactaattaa aacatcaaat catacgaata ttattttata aatgaataag
     5281 aattctacaa acttggattt acaaataaaa ttagcaacaa attcctatct aaatcaatat
     5341 gatatccagc tactttgttt tcaatttatt tactatttga atagcaattc tctgtaaaaa
     5401 cataaaaagc aacctcaact attatcttta aaatacacat ctatttaaaa actgtgcttt
     5461 cttgataaat atattgagat tgctcatcat attagtttat ttattttcgt tggaatatat
     5521 actaatccat tcataaatta gctttatccg agtttaaaat gttggtagtt aattatgttt
     5581 agatattatt cttcaacatc aatagattca attacaacat catgaagtgg tttatcttgt
     5641 ggtcccactt ttgtatttgc aatatcttct aatgtagttt caccatcaat gatttgaccg
     5701 aatactgtat gtttttgatc taaccatggt gtaccaccct tttcgccata tgcatcaacg
     5761 attggttgag gccagccacc atctgcaagt tgacttaaca tattttgagg tacttctttc
     5821 atttgaacaa tgaaaaattg tgaaccatta gtattaggtc ctgagttagc cattgataat
     5881 gcgccatata agttaaatgc atttaatgaa aattcatctt caaaagcacc gccataaata
     5941 ctttcgccac ccataccagt agctgttgga tcgccacctt gaatcatgaa gtcattaatg
     6001 acacggtgga atgtgattcc atcataataa ccattttttg catgtgtcac aaaattttca
     6061 actgtttttg gtgcaatatt tggaaataat ttgaatgtca tgtcaccttt atttgtgtgc
     6121 ataaccactt tgatttcacc ttgttgtact tctttgttta actgtggata gttagccatt
     6181 tatattctcc attcatgtta tgatacatat atattaacat acttttaagg gaggaaaata
     6241 gcatgaagct atatcgattt tcacacaaaa caggtagcta tggtgtcacg attgttgaag
     6301 aaaatggaga cgatgtcctt gttaaagttg aacaagttat caaacacccg aaacaaggtg
     6361 atttacataa ccctactgaa actgaaggtg tatttttcca tgaccgcaaa gcattaagcc
     6421 attttgaaaa acgctatacg aaacgttcac aattacgcga cttcaatgtt gatgtattgc
     6481 gctatgaaga ttcacttcaa caagcaatta cagatttaga aactaaactt aaagctcagc
     6541 aaacaaaaca tgctgaaatg tctttagcta gtttagcacg cttaaaagaa gattattcac
     6601 ttcaatacaa agaaaatttt aaataaaaaa tgacgtgttt cttcttattt aaatacttct
     6661 gaaaataaag cacaacaagg agtgtgtacg tttttcagaa ctatatcatt catgtataat
     6721 gaataacata aatatttttt aaaggaggac aacatagtga gtttgttaca tattgcggtg
     6781 atattaccgt taatattcgc actcattatt cctatcctgt atagattttt taaacgtata
     6841 catttaggat ggttcgtatt acctgtccct atcgtcatat ttatttatat gcttacatta
     6901 attaaaacga cgatgtcagg taatactgta atgaaaacct taaattggat gcctcatttt
     6961 ggtatgaact ttgatttata tcttgatggc ttaggtttac tctttagttt gcttatttca
     7021 ggaatcggca gtttagtagt attatattca atcggctact taagtaagtc agaacaacta
     7081 gggaattttt attgttattt attactattt atgggtgcaa tgttaggtgt cgtactttca
     7141 gataatgtta ttattctata tttattctgg gagctaacgt cattttctag ttttttactt
     7201 atttcattct ggcgcgaacg acaagcatct atatatggtg ctcaaaaatc gctcataatc
     7261 acagtatttg gtggtctatc attattaggt ggtatcatct tactcgctat accaacacaa
     7321 tcatttagca ttcagtacat gattcaacat gcttctgaaa ttcaaaatag cccattcttc
     7381 atttttgcga tgattttaat catgattggt gcttttacaa aatctgctca gtttccattt
     7441 tatatttggc taccagatgc aatggaagca cctacaccag tcagtgctta tcttcattca
     7501 gcaacaatgg ttaaagcagg gttatattta atcgctcgaa tgacaccaat cttcgcagca
     7561 tcgcaaggtt gggtatggac tgtcacactc gttggtttaa ttacattatt ctgggcttcg
     7621 ctaaacgcaa caaaacaaca agacttgaaa ggtatactgg cattttcaac agtatcccaa
     7681 ttaggtatga ttatggcgat gcttggaatc ggtgccatta gttatcacta tcaaggcgat
     7741 gatagcaaaa tatatgctgc tgcatttacg gctgcaatat tccatttaat caaccatgca
     7801 acatttaaag gtgccctatt tatgattaca ggtgcagtgg atcattctac tggtacacgt
     7861 gatgttaaga aattaggtgg cttactaaca attatgccta tttcatttac aatcactgtt
     7921 attacagcat taagtatggc tggtgtacca ccgttcaatg gtttcttatc aaaagaatca
     7981 tttttagaaa caacatttac agcaagccaa gcaaatttat tcagtgttga tacattaggc
     8041 tacctattcc caatcattgg tatagtgggc agtgtcttca catttgtata ttcaattaaa
     8101 tttataatgc atatattttt cggacaatat aaacctgaac aattacctaa aaaagcacac
     8161 gaagtatcaa tattaatgct tttatcacca gctattttag ctacattagt aattgtattt
     8221 ggtttattcc ctggcatttt aactaattcg attattgaac cagctacatc ttcgattaat
     8281 catacggtaa ttgatgatgt tgaattccat atgttccatg gattaacgcc tgccttttta
     8341 tcaacattag tgatttatat tttaggtatc ttactaattg tgacatttag ttattgggtg
     8401 aaattgttgc aacgacaacc tggtaaacta acattcaatt attggtacaa cagaagtgca
     8461 aatgttattc caaactactc tgaaaagatg acgaatagct atgttaccga ttattcacgt
     8521 aataatttag ttatcatttt tggtgcatta attttattaa catttgtcac tatattctca
     8581 gtacctttca acattaattt taaagacgtt agtccgatta gaattttcga agtttgtatc
     8641 gtaattttat tattaagtgc tgccttttta attcttttcg ctaaatcaag attatttagc
     8701 atcattatgt taagtgcagt tggatatgct gtgtctgtat tgtttatatt ctttaaagca
     8761 cccgacttag cattgacaca gtttgtcgtt gaatctattt caacagcatt gttcttatta
     8821 tgcttctacc acttaccaaa tttaaatcgt tataatgaaa agcgctcatt ccagcttact
     8881 aatgctttaa ttgctggtgg tgttggtttg tccgtcatta ttatcggttt aattgcatac
     8941 ggaaatagac attttgaatc gatatctaag ttttatcaag agcacgttta tgatttagct
     9001 cacggtaaaa acatggtaaa cgtcatactt gttgatttcc gtggtatgga tactttattc
     9061 gagtcatctg tgcttggtat cgcaggatta gctgtatata caatgattaa attacgtaaa
     9121 aagcgtcaaa cgcaaggtaa cgaggtgaaa aatcatgaat agacaacaaa atgatctaat
     9181 tttgcaattt gctgcagtta ttatcttttt catggtaatg gtctttggtt tttcactttt
     9241 cttagcagga cattatacac ctggtggcgg tttcgtcggc ggattattat tcgcaagttc
     9301 acttgttatc attacgattg catttgatat agaaacaatg cgtaaaattt tcccattaga
     9361 ttttaaaatc ttgataggta tcggattagt tttttgtatt gcgacgccta tcgcaagttg
     9421 gtttttaggc aaaaatttct ttacgcatgt cacatttgat ataccgttat ttatattaga
     9481 acctgttcat atgacaacag cagtattctt tgatttcggt gttttatgtg cagtagttgg
     9541 tactgtcatg acaattatta tttcgattgg agagaacgaa tagtggaaat tattatgatt
     9601 tttgttagtg gtattctcac agcaattagt gtctatctcg ttttgtctaa aagtctgata
     9661 cgaattgtta tgggaactac actattaaca catgcagcaa atttattttt aataactatg
     9721 ggcggactta aacatggtac tgttccaatt tatgaagcga acgtaaaaag ctatgttgat
     9781 cctatcccgc aagcacttat tttaacagca atcgttatcg cctttgcgac aacagccttt
     9841 ttcttagtat tagcatttag aacatataaa gaattaggca cagataacgt tgagagtatg
     9901 aaaggagttc cagaagatga ttgaaagtaa tatgcttgtg ttaacgctag ttattccagt
     9961 aattacagcc atattacttg tctttattgg aaagcgtcct attataaagc gttatgtcgc
    10021 actaggcggt acattattaa ccttggtcgc ggcaattatc aatttagcaa atgttgttaa
    10081 acatggacca atacgcgtag agctaggttc atggaaagca ccatacagta ttgtctttgt
    10141 attagacata ttcagtgccc tactaataat tactagtatt attattacag ctattgtgat
    10201 tttatattca taccaaacaa
//
LOCUS       DAKTIP010000091        10215 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAKTIP010000091 DAKTIP010000000
VERSION     DAKTIP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.091"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            102..305
                     /locus_tag="O0305_001635"
     CDS             102..305
                     /locus_tag="O0305_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274782.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            488..1423
                     /locus_tag="O0305_001636"
     CDS             488..1423
                     /locus_tag="O0305_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCY0274783.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(1505..2473)
                     /locus_tag="O0305_001637"
     CDS             complement(1505..2473)
                     /locus_tag="O0305_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0274784.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVKVDKHFGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2606..2950)
                     /locus_tag="O0305_001638"
     CDS             complement(2606..2950)
                     /locus_tag="O0305_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0274785.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(2992..3402)
                     /locus_tag="O0305_001639"
     CDS             complement(2992..3402)
                     /locus_tag="O0305_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0274786.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(3782..5848)
                     /locus_tag="O0305_001640"
     CDS             complement(3782..5848)
                     /locus_tag="O0305_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0274787.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVLLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(6149..7888)
                     /locus_tag="O0305_001641"
     CDS             complement(6149..7888)
                     /locus_tag="O0305_001641"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCY0274788.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELREFIEMAKIPVIHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAIASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(7935..8624)
                     /locus_tag="O0305_001642"
     CDS             complement(7935..8624)
                     /locus_tag="O0305_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCY0274789.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(8617..9012)
                     /gene="cidA"
                     /locus_tag="O0305_001643"
     CDS             complement(8617..9012)
                     /gene="cidA"
                     /locus_tag="O0305_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCY0274790.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(9225..10103)
                     /locus_tag="O0305_001644"
     CDS             complement(9225..10103)
                     /locus_tag="O0305_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0274791.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
BASE COUNT         3152 a         1959 c         1531 g         3573 t
ORIGIN      
        1 gaatctttac ttttttttaa agctaaattt acatcaactt aacaatggtt ggttatactg
       61 aagatgaaaa tatttagtat aacttagtgg aggcgataaa ggtgcaattg agtcattccg
      121 ttaaagttgc aatttctatc tatttagcac ttatctttat aacgttcact tcttatttag
      181 tcattatttt atatacgagt atgactggac atgatgtatc acatttcgtg ttagatagtc
      241 agcattctca tcatggatct cttacgcaaa aacatttgag tcttcctgaa atctcattta
      301 aatagtttat cctctgtgtt tcaacattca tttcccatat cgattcattt atctatcatc
      361 tagaccacta catcttagat gattttttta ttttctcatt tcactctttc ttaaaagtcg
      421 atataatgaa ttaaatcatt atcatacacc gacatatttt atgttgttgg tgttaagttt
      481 taaaggggtg agatacttgg cgaataatca ttcagctttg cgtttaaaag gaattatact
      541 tgccattgtc ggtgcttgtt tatggggatt aggtggtact gtttctgatt tcttgttcaa
      601 atataagaat attaatgtcg attggtacgt cactgctcga cttgtagtca gtggtgtttt
      661 cttacttatt atgtacaaaa tgatgcaacc caaacgttca atatttagcg tattccaaga
      721 tcgacgtatg ttaggcaaat tacttatctt cagtatactg ggcatgttag tagtacaata
      781 tgcttatatg gcatctatta atacaggtaa tgctgcgatt gcaacattac tacaatacat
      841 tgcgccagtt tatattatta tttggtttgt cataagaggc gttgcaaaac taacattatt
      901 tgatgtgctt gctattatca tgacactatt aggaacattt ttattattaa caaatggttc
      961 attttctaat ttagtcgtca atcctgcaag tttattctgg ggtattttag ctggtgtagc
     1021 actcgctttt tacacaattt atccttcaga cctacttaac cgcttcggtt cgattctaat
     1081 tgtcgggtgg gcaatgctta tttctggtgt tgcgatgaat ttacgccatc caatttggca
     1141 cattgatatc actaaatggg acatatcaat tatattattt ttaatctttg gtattatcgg
     1201 tggtaccgca ctcgcatttt atttctttat cgacagttta caatacatat cagcgaaaga
     1261 aacaacatta ttcggaactg ttgaacctgt cgtagccgtt atcgcaagca gtctatggtt
     1321 acatgtggca ttcaaaccat ttcaaatcgt aggcatcatt cttattatga ttttaatttt
     1381 attactatca cttaaaagac aacctgaaac attagatgaa taagaaaact ctgataatca
     1441 ctttagcaag taactattat ttaacaacgt agttacctta taggtgatat cagagttttt
     1501 tattttagtt aataatattt ttcacttggt ataaaaagcg tcgtcgctct ggtatcggaa
     1561 atactggata aatatggaat tggtaatatc caggtataaa gtcatgttcg atacctttcg
     1621 cactcaacaa ttgcgataag ttcaaagcat ctgggtacag tacttctttt gtaccaacag
     1681 ttaatgtaat acgacctaaa ccatctaagt caccattaat tggtgatact ttgtagttat
     1741 ctaaaggtgt gtcccctgcc cattgttcag ctaagaacac actgccatcc acacctacca
     1801 ttgggtcttt ctttaagtag tcaggaattt caggatgctg catcgttgca tctaaaactg
     1861 gtgaaattaa tacaatatgt cctggttgca caatatgttt ttcttttaac aattgagcaa
     1921 atgataaagc aatttgaccg cccgcagagt cacccattac aacgatttgt ttagaatctg
     1981 ctacttgatt caataaatca tggtacaact tttcaaaaag cacatacgtc gcttgataat
     2041 cttgatgcgg aatcttcgga tatactggca tgatgacttt agcattgagt gtttctgcaa
     2101 gttcatcaat aaattcgaaa tgaattttga gtgggtcttg gaaccatgcg cctccatgtg
     2161 catataatac aacgcgttga tgcttatctt gtttatcgtt aacggtataa acggttgaac
     2221 cgaagtgctt atccactttt actggcatct taaactgata attggttccc tcatattcgc
     2281 ctgctgtctg tggcgccatt ttttctaatg ctttcatcgc ttcttcttta tacataaaac
     2341 gacgcttagc tctagataat cgtatacctt gttctaacat ataacttttc acactgcgtt
     2401 tttctttaat tctaatatgt gcgtatgctg caactaaaaa tccaaacgca agtgtagacc
     2461 attttttacg catttgtcgt cactcctctt tgatgatttc tactatagtt ctactgtacc
     2521 atgctgattc taattactac caatatttac tgggaaataa gttatgatgt ctacataatc
     2581 ttagcaatgc taatcatttg cgatattaat gctcatcgac atcgttattt acactatcat
     2641 tcatttgctt aagatgatgt tcaaaacgtt gcatatcgat aaaacgccct tgacgatgca
     2701 tttcttttcc attcataaaa attaaatcca caggtacagt gaaaatattt aattctccag
     2761 caatagcttc cacctgactt tgattaatca cagctaatgg cacattggga tatgattgca
     2821 tcaagtcttc aatttgtggt aaaacggcat gacacacgct acactgatcg cgcatgacat
     2881 gtacaactgc taacggatat tgatgaataa attcacggaa tgcttcgaca ctatttaatt
     2941 gcttaaccat ttcgaacacc ttcttacttt ttatatttta aaatgaagtc attattttgt
     3001 tgctaaaatg tcttgaaaca cttgtggcac agaggttatc ttcttatcac tcaatctgta
     3061 aaaaccgcct gtctgtgtac caatacaatg cacgccatgt gcggacacaa tttcaaactg
     3121 aaacacccat ttcatcttgc ttgctttaac taagtaaata cgacctactg gtttgtcatg
     3181 tattgtgaca ggtactttat actctgcttc cgttttcata agtataggtg aaatgccttg
     3241 attgatcatt gttgagtaat tcatgtgctg attaataaac gctgttctca aatcttcgaa
     3301 ccatctaata taaacgatat tactaacgac acccatcgca tcaatgtcgt aaccattcac
     3361 ttcgatttct ttctctgcaa ctaatagacc ttctgttgtc ataatgacaa atctccttat
     3421 cttacaaata gatttaaaat catgttaatt tatttttatt actttcatga taaaaataca
     3481 agcaattgaa ctttattacg gaaaatcaaa cttttcaatg agactattaa tttggctagt
     3541 cgcgaagatg catttttcta aaatgtgaca aaaacataag caatgttttc tatatctaac
     3601 actaatattt aacttattat catcaacaca cctgctatac atcattgcgc actaaaaagg
     3661 cagtagcttc attttaagct actgcctttt gtaacgtatt ctctttcaac tcgctacttc
     3721 gcatacgcac tacgaatgac taaaagttca ttcatttgat atgctgtttc atttttaatc
     3781 attatttgac tgtcataatt agcttacctg gatcagcatc ttgtacatct tcaatgacaa
     3841 gttctttatt ttcaagattt gtaataatca ttggtgtaac gatgcttggt gcgtgtgctt
     3901 tcaagtatgc taaattcact ttcattaatg gttgaccttg tgttactttt tcatcaacgt
     3961 taatcagact ttcgaatcct tcaccattca gtttcactgt atcgatacca atatgaataa
     4021 gtacttcgac gccactttca gattctaatc ctatcgcatg tttcgtaggg aagattgttt
     4081 tcactgtacc atcaaacggt gctacaattt cacctttttc agggataaag ccaacaccat
     4141 cacccatcat tttaccagcg aacacttgat caggtacttc tgataatgga atgatttgac
     4201 cgatacctgg tgcatagatg tctgttgttc caagtgcttc tacgtgaact ggttcatctg
     4261 acatttcttc agtcactgtc gtttcacttg gtttcgtaat ttcaccactc ataatcttgg
     4321 ccatatcatg tttaatttga tctgattttg gaccaaagat agcttgcata ttgtttccaa
     4381 cttctaatac acctgatgcg cctaaagctt taatacctgc tacatctact tttgatttat
     4441 caaccacttc tacgcgtaga cgtgtaatac atgcatctaa atgtttaatg ttttcttttc
     4501 cacccattgc atctaagaca tcaaatggta attttgcgac actagagtta cgaatttcag
     4561 tttcttcatc ttcacgacct ggtgttttca atttaaactt acgaattgca aagtcgaata
     4621 agaagtaata cacgatagca tatacaatac cgactggaat aactaataat gcgtgtgaac
     4681 gatcccagtt taataaacca tataaaatat aatctatgaa accacctgag aatgtcatac
     4741 caattttaac gcctaataaa tgcattacta agaatgatgt accagctaat aatacgtgaa
     4801 ttccataaag tactggtgct acaaataaga atgaaaattc taatggctca gtgataccag
     4861 ttaaaaatgc agttaatcct gctgataaca ttaaaccacc cacgactttt ttacgttctg
     4921 gtcgtgcatt tttataaata gcaaatgccg ccgctggtaa accaaacatc ataaatggat
     4981 atttaccagt agtaaatgca ccagcagtaa atggtacgcc atctttcaat tgtgccatcc
     5041 aaatacgttg gtcaccacga accaattcac ctgcgtgatt tgtataactt ccgaattcaa
     5101 accagaacgg tgaatagaaa atatgatgta aaccaaatgg aattaatgag cgttcaataa
     5161 taccgaatat aaacgttgtt aatgttaaat ttttatttaa taagaaattc gataaactat
     5221 ttaatccatc ttgaattggt ggccacgcaa agcttaaaag cacacctgtt gcgattgcta
     5281 cgaccgatgt cacaatcggt acaaatcgtt tacctgcaaa gaatcctaaa aatggtggta
     5341 gtgtaatatt ataaaatttg ttgtaacacc atgcggctaa agcacccatg ataataccac
     5401 caaacacacc cgtttgtaac gttggaatac ctaatactaa agcatgtgct ggttctttcg
     5461 ctgcttgact taattctttt gcccctttgg catatgagaa aatgtcatca attgtaatgt
     5521 gcaacacttt ccccattgtt gcattcataa ttaagtaacc tactagcgct gctaatgctg
     5581 caacaccgtc tcctcctgct aatccaagtg ctgtaccaac tgcaaataat aatggcaagt
     5641 tatcaaatac aacttgtcct gctgcttcca tgaccgacga aatcattaca atgatatcgt
     5701 tttttaacca tggtgcaatt tctactaatt gttcgttgtg cattgcgtta ccaaacgcta
     5761 ataaaatacc agctgctggt aaaatcgcaa caggtaacat taatgcttta ccgatacgtt
     5821 gcaattgtcc aaacaatttc ttaaacatat aaacatcccc tatctcaatt tccgcttcca
     5881 tgtacaaaaa aagcaggcat gaacaaacaa gactattctc atagtgcggt tgtttgctca
     5941 tgcctgcatt accagtaaca cgcaagtgat atctaattca tagctattat acatatttgt
     6001 aaaagcgttt tcaacaactt tttcacaatt ttatcatcaa ttcagtacga tcatttgtga
     6061 taaataaaca aaacggcaat tcccattttg ttgaaataga aattaccgtt tttatagttc
     6121 aattcaattg tgataacctt taaatcagtt ataagaaacg ttttgctgcc actgaaattg
     6181 gtggaatttg atctaaatct aaatgtttat cttcagtaat tgatctaaat gcccacttac
     6241 cataaccaag cgcttcttca tttacaattt tacctggtaa tggcgcagca ttaggatcaa
     6301 catatacatc tacaatcgtt ggtacatctt gtgctaatgc ctcttcgact atagcatcta
     6361 cttcgctagc actcttaatt gtataacctt taccacctgc tgcctcagca aattttgcat
     6421 gatccatatc agaaaaatca actgcatatt ctaattcacc agctgcttgt tgttcatatt
     6481 taataaatgc taactgtttg ttattaagta caaatacagt taaaggtaaa tcatattgta
     6541 ctgctgtagc gaagtcttgc attaccattt ggaatgcacc gtcaccagca attgcgatgg
     6601 cttgtctatt tggatatgca atttttgatg caattgcacc tggaagaccg caacccattg
     6661 tacctaacca acttgaaatg atgaacttgt tatttacacc aaggtttaag tatcgagttg
     6721 accaaactgt tgctgtacct acatctgctg aaatcactgc atcatcttta ataaatttat
     6781 tgattgatgc cattaatcgt tctggacgta atggtttact attattattt ttatcttgtt
     6841 ccatccattt atcccaaacc gctttacgtt ctaacgtttt gtttaagaat ggtctttcag
     6901 caacatgttt aatattttca gttaactgat gcaacgcaat tttactatct ccaacaattc
     6961 ctacattaat attgaaacga tgtccgatat ttttaggatt tgtgtcaatt tgaatggctt
     7021 taatattttt cttaggtaag taatccacat atggatagtt tgtaccaacc ataattaata
     7081 aatccgcttc ctgcattgtt tgataagatg ttttggtacc gattttacct aagttaccga
     7141 tactatatgg atgatcatcc ggcaagattg ttttagctgg taatgaatga atgacaggaa
     7201 ttttagccat ttcaataaat tcacgtagct catctttcgc atgtttcgca cctacaccaa
     7261 ttaacatgac aggcttttta cttttattaa ttagtttaac cgcttttttg atgtctttat
     7321 attttggtga tactactgtt ggtcttgatg tatctactgg tttattcgtt gtatctttaa
     7381 ttttttcagt taataagtcg ttaggacaaa taacaacagc tacacctttt tgttcatatg
     7441 ccgtacgaat tgcttcgtta acgatttcaa acacattgtc acctttttca atttggtgat
     7501 tataaacggc tacatcttca cataattttt gtaaatttgt ttcttggaat gctttcgttc
     7561 caagtgctgt actattcgtt tgtccagata atattaattg cggtacatta tccattttgg
     7621 catcatacat accatttaat aaatgaatta aaccagggcc accgatactt aatgccacac
     7681 cgattttacc agttaatttt gtgtaaccag cagccgctaa gcttgctact tcttcatgac
     7741 gtacatgata aaatttaaat tgatctctca ctgtacgtaa actatcgact actgcgtcga
     7801 ttgagtctcc tggaatacca tacaagtgat ctatatccca tgcttgtaat gctttaacta
     7861 atgcttcatt tgcttttatt tttgccatta ttactaatag cctccctttc tgtctatcat
     7921 tttaaatatc taaattaata gaataataaa attagaacag gtattaacac tgaactaata
     7981 actgcagtta aaatcatccc aattgaacta aatgcaccgg attcaatatc catttctagt
     8041 gctttagctg tgccaaatgc atgtgacgca ttcccatacg ttaatccttt ggcgatagaa
     8101 gattcaaatc ttccaaatct taataacatc gaacctaaaa tactaccgat taaacccgtt
     8161 gtgataataa aaagtacggt catcgtatct gtaccaccta gttcatgtga cacttcgata
     8221 cctactgcgg ctgttataga tcggggcaat aacgttacaa tgacgtcttt agaatagcca
     8281 aatgctttaa gtgttaagaa cactaacatg aaattcagca tgacgccagt taatacactt
     8341 gcaaaaatga tagagacatt gtctttaatt ttctctctat ttttataaag tgggtacgct
     8401 aaacatacga ccgttgcgtt taaaatatgg ttgatccaac tgccaccttt catatacccg
     8461 ttataactaa ttccaaagat aagtaagaca aaaataattc ctaaagatgc aattaatgct
     8521 ggattcaaaa atgggttcgg atatttttgt tgtaaccttt tagcgaaata atataaaacg
     8581 acagtcaaca aaatcattaa taaggcttgc acgtaatcat tcataagcgt ctacaccttt
     8641 acgatgttta tgtttaacag acattttttc agcaatataa cctgaagata atgcaacgat
     8701 acatgttcct atgataatga ctgcgaaaaa gagtatataa tttagcgtaa tttcggaagc
     8761 aacatccata atacctacaa ctgacggtat gaagaaaaag accatcgtct ttaataaaaa
     8821 gtttgcaccg tcttctaccc aagttagcgg tacaatctta aattgtaata gtaaataaaa
     8881 taaaaataga ccaacaatac tgccggctaa gggaagatga aaaatctttt gaatttctgt
     8941 gccaatataa gtaatcacaa tgatgattcc tagttgtagt agtagtttga ttattaattg
     9001 gactttgtgc atggcgccat ccctttctaa atatgtctaa attgttacaa taactattat
     9061 aaagatggcg tacacaatat aaaaatactc tttttttata gtttgtatta ctattttcta
     9121 tggaaacgct ctctaaattt cacttcattt tcacaaaaaa acactcatta tttcaatgac
     9181 ccacgtaatg atgcgcctta aataatgagt gctttattaa tatgttagcc taaacggtct
     9241 ttcaaaaatt ctatccattt cgttgtggca tgacttaatt gtttatcctt cttccaaaca
     9301 acacctaatt cccaatgtac atgagcatct tcaatgcgta gcagcttcac atctccattt
     9361 agttgctctg aaattgatgt tggtaaaata ctaataccta attcattcgt aactaaatct
     9421 tctataacat gccattgtga aatttgcgct acagtattcg gaacaaagcc aacgttttta
     9481 gcattttcaa taattttatc attcaagtaa aagtctttat taaataaaat gaagtcttca
     9541 cctgctaaat cttcgagttt aacagtttca tattttgcga gtcgatgctc tctgctcacg
     9601 ataagtcgca aatcttcctt atctagggta gtataatcga aaatatgatg atcgactggc
     9661 aaagtggtca cgcctatatc tacttcatca ttgataattt gctgttcaat tgttttaccg
     9721 ccattttcta tcaaattata tgtaacattt ggatattgtt gatggaatgc accaagaata
     9781 ttgataagaa tcttcatatt catgactgcc gacatgccca tgtttatatg tcctgtttcc
     9841 agtccattca agcgttccat ttcagatggt aaataatcat acagtgcaac aatttcttta
     9901 cttttctcat aaaaaatttg acctgcatcg gtaagaatta aatgtctttt acttctatca
     9961 aataagggcg tgcccatttc attttcaata tctttaattg ctttactaat tgtaggttgt
    10021 gcaatatata atgatttgga agcattagtc atgcctcctt gcttaacgac ttcaataaaa
    10081 tatttcatat gtttgatatc cactaacatc atcctaacta tttcaacacg tttatttcat
    10141 tatacacaac ttcttaaaca ttcaataaat ttagatttat aaaattgctt tataaaaagt
    10201 tttatgtaaa ataca
//
LOCUS       DAKTIP010000092        10143 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAKTIP010000092 DAKTIP010000000
VERSION     DAKTIP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.092"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(916..1359)
                     /locus_tag="O0305_001645"
     CDS             complement(916..1359)
                     /locus_tag="O0305_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0274792.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(1524..1901)
                     /locus_tag="O0305_001646"
     CDS             complement(1524..1901)
                     /locus_tag="O0305_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274793.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPFTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFF"
     gene            complement(2219..2809)
                     /locus_tag="O0305_001647"
     CDS             complement(2219..2809)
                     /locus_tag="O0305_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCY0274794.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(2806..3018)
                     /locus_tag="O0305_001648"
     CDS             complement(2806..3018)
                     /locus_tag="O0305_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCY0274795.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            3079..3625
                     /locus_tag="O0305_001649"
                     /pseudo
     CDS             3079..3625
                     /locus_tag="O0305_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3720..5336)
                     /gene="groL"
                     /locus_tag="O0305_001650"
     CDS             complement(3720..5336)
                     /gene="groL"
                     /locus_tag="O0305_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCY0274796.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(5412..5696)
                     /gene="groES"
                     /locus_tag="O0305_001651"
     CDS             complement(5412..5696)
                     /gene="groES"
                     /locus_tag="O0305_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCY0274797.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5871..6614
                     /locus_tag="O0305_001652"
     CDS             5871..6614
                     /locus_tag="O0305_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0274798.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(6639..7892)
                     /locus_tag="O0305_001653"
     CDS             complement(6639..7892)
                     /locus_tag="O0305_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274799.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDNPKPNPDPKPDP
                     NKPNPDPKPDPDKPKPNPNPKPDPNKPNPNPSPDPDQPGDSNHSGGSKNGGTWNPNAS
                     DGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEV
                     TDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGE
                     IGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMM
                     VVFSIMLFLFVKRNKKKNKNESQRR"
     gene            8089..8715
                     /locus_tag="O0305_001654"
     CDS             8089..8715
                     /locus_tag="O0305_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCY0274800.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            9076..9861
                     /locus_tag="O0305_001655"
     CDS             9076..9861
                     /locus_tag="O0305_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCY0274801.1"
                     /translation="MKVQIYQLPIVFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGEYVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT         3271 a         1809 c         1439 g         3624 t
ORIGIN      
        1 acatttatac caccaaaaag tcctaaaaat taccgctaca ctaaaggaca aatttatcta
       61 taaaataact atccatcttt aataagaaat gaactagtgt tttaatctat tacattaaaa
      121 aaactaccta aacaggtagt tttttattta ttttaggccg attaaatgtc ttatgtcttt
      181 aattaagtaa aatgcacaag tacaccttta aaaagattta ctcaaaacta aactatacct
      241 agttaaccgc ttgtgattaa gaaattttaa atgtggtttc ttttaattat gtcaaccacc
      301 cataaggatt ttaatcaagc actcattcat tcctgcactc gcaatatgca ttacttatag
      361 aagtggatgc actctcatat tatggcaata ccataactcc cgcaccataa tgatttaaat
      421 accattatga atctcaatta caaattagca cataaaaaag acgcctccca taagtctagt
      481 caccaaatgg aaaacgtctc gtaattaaaa tatatttgcg tgcattctaa tttgtactta
      541 gaatacatca tactcgttca taatgtaatt ttctaattag tcaaaactat atacagtttt
      601 acatcattcc cggcattatg cactaataaa aacttacttt aatacacgct ttaaagatta
      661 ttaaatcagc gttttaattt ttagacaacg ctattatttt tagtttttca attctattat
      721 gtcataatta tgtcactcaa aaactgtttt ccaacattga tttattgttg gaaaatctcg
      781 aaaatctagt ctatttctcc aacaatcgaa gaattatgcc cttttttctc cctttaaata
      841 agtcataata cgaggcatac atgcaacatt tacaataaaa tatgttccta catcgtatta
      901 tacgaatgct cacacttata gttgtttagt aaacccagat gtattgctaa catacccatt
      961 atcactatat agtttatgtg cagatagtct ttcatttcta ttaccactat ttagtgttat
     1021 tgctttacaa ttcaaccgtt tagaaaattc ttcagaatca gctaataatc tctttccata
     1081 accttttttc ctaaattcag aatgtataac aaacgcaagg attctcatat actctgcatt
     1141 tttttcgtaa aacatcattt tacacatacc acttaaacca attattttat tttcttttat
     1201 caaaagcagt aggaagtaat catcatgatt cgttattttc tttagtctct tttttaaatc
     1261 attctcattt gttggataac ctagatcatc atataattta catagttgat ttaatttttc
     1321 aaaatcactg ttattaaaca atcttgtaat tatgctcata taacccaccc taaaaatatt
     1381 atgtacctat tgttacatac aatagatacg caaaatcatt tctcactgca aatcaggacg
     1441 tttctcagcg taaacaaatc atttctattc ttttgaaaaa tatctgcata tcttcacagt
     1501 aattttataa cagattaata aaattaaaaa aacataacaa caagtattat aataaatatt
     1561 ataatagcta tcgtattaaa tagtttagaa tatgcaaact gaaaaataaa aatcacaatt
     1621 aaaatactaa gtaatgtaaa tggtaatagc atcatcagat ctttaacatt atttctttgt
     1681 tcttgctccc aatcgctaat agccctaaaa ctactttttt tatctatagg cgatgtaaaa
     1741 ataccatata ttgaaggtgc tatacctcct aaaatagcag ttcccaaagt tgtcattact
     1801 gaaattactg cgaaagtatc atccgaaagc aataaattca aactaatgca ttgtttatta
     1861 cccatcgaat ttattgacca aatagctaga gaaataaaca acccaaaatt taaaataaat
     1921 gatatagtaa tagcaattgt ttacaaaaca cgaaattttt catttttatt tatattatcc
     1981 atttttctcc ctttttctta aatcatttta ttatatatta caataatcaa tctgaaatgt
     2041 tgatgtaatt tgaaaaaaat atcatacttt tactcctgaa aacctcccta aatcatcaat
     2101 atgaaaatca gtattaggat attgcgttta caactctttt aaatctcact cattcttctc
     2161 atcgtcttaa ccgatactat caataaatac tggcaacacc ataaaatata tacccaattt
     2221 ataaaaacct aattgactca taatcttcta acggtatatc atccacaatc acagtatgat
     2281 taggattatc gttagataca tctttcactg ccttatctaa cccctcatca cctccgtccc
     2341 attcaccaat attaatgaat ataggtacat taccgtttat atcatgctta tctgtaaata
     2401 atttatgata tttacccaac atatcacgag cttttaaacg atcactaggt tttattggta
     2461 cctctatcag ttcaacatgt tcattataga ctaattgtac tttgccactt tgtggatttt
     2521 ctttatattc tccacgcttg actacaactt ctttcgtttc tgtttcatca ccgactgccg
     2581 cattcgttag cacatgtaat aactcttttg cggttaatac attctcatct ataatcttat
     2641 ctttttgttc ttgtatatat tgcttgatgt gcggcttttt caataaccta cacgctgtca
     2701 catgtgcgct atttgcgtta tatcctgctt ttatggcact ttgtgttaca ttcagtgttc
     2761 taatatactc attcacaaaa cgtgcttgct ttgcagttaa ctcactcatt ttatcacccc
     2821 cacaatttta tctaatatgg tttcatacca taatattaca gattgttctg aacaatctaa
     2881 ggcactacta atatcttgat aactaagtcc ttgtataagg gagtcaaaaa tataaaactc
     2941 tttatcggtc gctaatctgt caacaatcat ttctatgtga ttctttataa tatgatcatt
     3001 gacattatcg tctgtcatca attcgtcaga atcttcatca cctattaaaa agaaatcatc
     3061 agtatttatt tcatcatcgc cccgttaact agctttgaag tctttagcac acttgcatat
     3121 accggctgtc gtgctggcga gatactagca ttgaagtggt ctgatattga ttttgaaaac
     3181 aacacgatta gtattactaa aacatattac aatccaaata ataacaagaa gaaatatcag
     3241 atacttcccc ctaaaactga aagttctatc ggtaagattt ccgttgaccc aaatgtaata
     3301 aaggtgttgc gtgattataa gataaacgtt caaaataatt ggaaaaacga attatataat
     3361 gataaccatt gtaatgaaaa aactatcaat gtggatacaa accattatgt cacgtaccag
     3421 catactcaaa aatatctcta cgcactcatt cagatacaca cattgcgcat tgctcataga
     3481 atcaggtgta cacattaaag aaatacaaga acgattacgg cataaagata tcaataccac
     3541 tatgaatatc tatgctaaaa tcacaaattc atacaaaaaa gacgcctccc ataagtttag
     3601 tcaccaaatg gaagacgtct cgtaattaaa atatatttgc gtgcattcta atttatactt
     3661 agaatgaatc atactcgtgc ataatgtaat tttctagtta gtcaaaacta taaacagttt
     3721 tacatcattc ctggcatgcc acccatgtta ggttggtcat tatttttttc tggaattgat
     3781 gctacaaccg cttcagtcgt taagaacatt gctgcaacac ttgcagcatg ttgtaatgct
     3841 gagcgtgtta ctttagttgg atcaacgata ccttcttcta acatattaac ccactcgttt
     3901 gtagcagcgt taaaaccaac acccggctct gcgtttttca aacgttctac aataacagaa
     3961 ccttctaatc ctgcattttc agcaatttga cgaactggtg cagttaatgc tttaagtaca
     4021 atatttacac ctgtttcaat gtcaccttca gcttcaattt cacttacttt ttggtaaaca
     4081 tttactaatg cagtaccacc acctgcaaca ataccttctt caactgctgc acgtgtagaa
     4141 tttaatgcat cttcaatacg taatttacgt tctttaagct ctgtttcact tgctgcacct
     4201 actttgataa ctgcaacacc acctgctaat ttagctaagc gctcttgtaa tttttcacga
     4261 tcaaagtcag attcagtttc ttcaatttga gatttcaatt ggctaacacg tgcatcaatg
     4321 ctgttttcgt caccgtcacc atcaacaaca gtggtattat ctttagttac ttctacttta
     4381 cttgcagtac ctaacatatc aattgatgca tcttttaaat ctaagcctaa atcatcagta
     4441 atcacttgcg caccagttaa aatagctaaa tcttcaagca tcgcttttct acgatcacca
     4501 aaaccaggtg cttttactgc aacagctgta aatgtgccac gcatacggtt tagcacgata
     4561 tttgttaatg catcgccttc aacttcatca gctacaatta agattggacg attagattga
     4621 accacttgtt ctaataaagg taagatatct tggaaagacg agattttctt atctgttact
     4681 aaaatgtatg ggcgttctaa ttcagcaacc attttatctg aatcagtaac catatacggt
     4741 gattgataac cacgatcaaa ttgcatacct tcaaccactt ctagttcagt gtttagtcca
     4801 tttgattctt caattgtaat gacaccatcg ttacctactt tttccatagc ttcagaaata
     4861 taacgtccaa tttcttcatc tgctgctgaa atcgcaccta cttgcgcaat ttcattttta
     4921 ttttcaactt tttgagaatt ttcatgtaac gcttcaacag caactttaac tgctttgtcg
     4981 ataccttgtc gtaaaccaac tgggttcgca ccacttgtaa catttttcaa gccttcttga
     5041 atcattgctt gagctaatac tgttgcagtt gtcgtaccgt caccagcaat ttcatttgtc
     5101 ttatttgcga cttcttgaac tagtttagcc cccatatttt catatggatc ttctaattcg
     5161 atttctttag caatcgtcac accatcattc gtaattaaag gtgctgtaaa ctctttatct
     5221 aatacaacat tacgtccttt aggaccaatc gttactttaa ctgcatttgc aagttggtca
     5281 acaccacgta acattgcttg acgtgcatct tcagagaatt tcaattgttt aaccataaat
     5341 gataaacctc catttgttta gttgtgtttc attttcgttc tatttaattt atttatgaat
     5401 taagttctgt attattcaat aactgctaaa atatcttctt catttaatac cagatatgtt
     5461 tcattatctc gtttaacttc tgtaccagca tattgttgga acacgacacg gtccccttct
     5521 ttcacttcag gagtcactct tgtaccatca tttaataggc gtccagttcc tactgcaacg
     5581 ataacgcctt cgtttgattt ttctttagca ctatcagtta aaacaatacc acttttagtt
     5641 gtttgttctt gttctttttt ctcaataatc acacgatttc caattggttt tagcatgatt
     5701 gttcctcctt aaaaaaccta aagtttagca cttaacatta aagagtgcta acatacattt
     5761 ataataatca aatttggtca aaatttcaag tcataacctt ttaattaagt tttgtaactt
     5821 caagttattt acgataaaat aaattataaa caaatatttg gaggaaaatt atgacaagat
     5881 tatgggcatc attgctaact gttattattt atatattgtc tcaattttta ccgcttctca
     5941 ttgtaaaaaa attaccattt gtacaatata gtggcataga actgactaaa gcagtcattt
     6001 acatacaact tgttctattt ttaatcgccg ccacgacgat tattttaatt aatttaaaaa
     6061 ttaaaaatcc aacaaaatta gaattagaag ttaaagaacc taaaaaatat atcattccat
     6121 gggcattgct tggatttgca ttggtaatga tttatcaaat ggtagtgagc attgtattaa
     6181 cgcaaattta tggtggacaa caagtaagtc ctaatacaga aaagctaatt attattgctc
     6241 gaaaaatacc tatatttatc ttctttgtat ctattattgg tcctttatta gaagaatatg
     6301 tattcagaaa agtaatcttt ggagaattat ttaatgcgat taaaggtaat cgtatcgtgg
     6361 catttattat tgctacaaca gtaagttcat taatatttgc attagcacat aatgatttca
     6421 aatttattcc agtttatttt ggtatgggtg tcattttttc attagcatat gtttggacaa
     6481 aacggcttgc tgttccaatt attatccata tgttacaaaa cggatttgtc gttatattcc
     6541 aattacttaa tcctgaggct ttgaaaaaag ccacggaaca agcgaatttt atatatcaca
     6601 tttttattcc ataataaaaa aaacgaatca cacacggatt atcgtcgctg tgattcgttt
     6661 ttatttttct ttttatttcg ctttacaaat aagaatagca tgattgaaaa taccaccatc
     6721 atagaacata caattgctag ccaactttta ctatatgcag ttgacgcact tttcttatta
     6781 tcttcttgtt ttttaactac tgtaaaatcg tctttttcat gcggttcaaa tgagcgttgt
     6841 tttgaatccg gcttatcttc gggctttttc aaatctggca ttgatccaat ttcaccagta
     6901 attaatgcga gtacttgttc atctaaatta cgatagtacc tttctgattt caatggattg
     6961 aaatattgga atctaaagta attcgattgc tgttgtaatc catttaaata tgcatttccg
     7021 ctgaaatttt gttttcgaat aatattataa atgtcttcat cagtaacttc tccataatct
     7081 ttgcccaact tattaatttg atttaaaata tacggattat acttgtaagc cccatttgcc
     7141 aaggctaaaa atcgttggga tacaaaatca ttaccagtag gattttgtga gtttccttga
     7201 ttcccatttg gttgccattg accttgatta gatccatctg aagcatttgg gttccatgtc
     7261 cccccatttt tcgagccacc agaatgattg gaatccccag gttgatcggg atctggtgac
     7321 gggttaggat ttggcttatt agggtctggt tttggattcg gatttggctt tggtttatct
     7381 ggatctggtt ttggatccgg atttggctta ttagggtctg gttttggatc tggatttggt
     7441 ttcgggttat ctggatctgg ttttggatcc ggatttggct ttggtttatc tgggtctggt
     7501 tttggatccg gttttggttt cgggttatct ggatctggtt taggattcgg acttgggttt
     7561 tggttatttg ggtctggttt tgaatctgta cttgggtttt ggttgtttga gtctggcttc
     7621 acattattcg aatcaggatt agcatcacga ctattcgaac ctgttccttc attacccgtt
     7681 tgatttgatt cgtgaggtgt cccagtctca ttattattca tttcactatt tactgatggt
     7741 gaagttatat tatgattcaa attagtagtt ggtttttctc catttaagtt atcctttgct
     7801 tcaactgtgt gcgttgcaag ccctaaacta ctactactta aaattaaaat acttgttgaa
     7861 agtaacattt tcttaaacca atgatatgac atttcacgag acacctccct ttttcatgta
     7921 agtattaatc taagtataat aaatatgcgt tgaaaatgca tttatatttg gaatttgaaa
     7981 atgaatatta atattcttga aattttcgta aatattgctt tacaagtacg agtataaatt
     8041 ttatacttag tctattaagt attctttact aaagaaggag caataattat gggcttattt
     8101 aaaaaagata aaaaagcaat gacttttgac aatgcaatgg aagaaagacg ttcaatatat
     8161 aatttgaaag attcgatatc aattagcgat gacgaattag aatcaatgat tgcacatgca
     8221 gttaaacatg ttccttcatc atttaattct caatcaacta gaattgttct cttgttaaat
     8281 gacaacaata ataaattttg ggataacaca aaagcaattt taaaagaagt catgggagag
     8341 aatcgtgatt tcgagccaac tgaacaaaaa attgataact tcaaacattc atacggtaca
     8401 attttgtttt atgaagatca agatgttgta agtggtttac aagaacaaat gccaaactac
     8461 tatgataatt ttgcaatatg gtcaactcaa acaaatgcca tgcatcaatt tgcaatttgg
     8521 actgcattag ctactaaagg cataggtgct tcattacaac actataatcc acttgttgat
     8581 gagatgactt caaatgaatt taatattcct aaaagttgga aacttattgc tcaaatgcca
     8641 tttggtgata ttcgtgaagc tgctggcgaa aaaacattta atccagtgga agatcgtttc
     8701 gtaataaaaa aataatcaat atattgttag gaatgggaca gaaatgataa agaatcatta
     8761 gttattcatt atgtagtagt tcttacacat tagccgctgc taatgtgcac ttaagaatag
     8821 gaatacatga gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt
     8881 tatgttagag cccaccccaa cttgcattgt ctgtagaaat tggtgttcca atttctctat
     8941 gttggggccc catcaactac tgtcaatata actttgtaga gcctaggact ttgattttat
     9001 gtcttgtgct cgttttttgc gtttcaacat aacttttagt aagttatata taagatctat
     9061 caaggatgtg atgttatgaa agtccaaatt tatcaattac cgattgtttt tggagatagt
     9121 tctaaaaatg aaactcaaat aacgcaatgg ttcgaaaaaa atatgaatgc tgaagtagat
     9181 gtagtcgttt tacctgaaat gtggaataat ggctatgatt tagagcattt aaatgaaaaa
     9241 gcagataata atctaggaca aagtttttca tttattaaac atttagctga aaaatataaa
     9301 gtcgatatcg tagctgggtc agtttctaac attagaaaca atcaaatatt taatacagct
     9361 tttagtgtga ataaaagtgg ccagctgatt aatgaatacg acaaagttca tttagttcct
     9421 atgttaagag agcatgaatt tttaaccgct ggcgaatacg tagctgaacc ctttcaattg
     9481 tctgacggta cttatgtgac ccaacttatc tgttatgatt tacgttttcc agaattatta
     9541 agatatcctg ctcgtagtgg tgctaaaatt gcattttatg tggcgcaatg gcctatgtca
     9601 cgtcttcaac attggcattc attactaaag gcacgtgcaa ttgaaaataa tatgtttgtc
     9661 attggaacta atagcactgg attcgatggt aacacagaat atgctggtca ttcgatagtt
     9721 ataaacccaa atggagattt agttggagaa ttaaatgaat ccgcagatat tttgactgta
     9781 gatttaaact taaatgaagt agaacagcaa cgcgaaaata tacctgtatt taaaagtatt
     9841 aaattagatt tgtataaata agaagcgccc gaaataatat ttaacacatg tcatgactaa
     9901 acatagattt atgagagatt tttcataagt ctatgtttat ttttttataa tagataactc
     9961 agtaagaacc catttcgccc tttgcaaatg aatgattttc ttaattaaat tgattaattt
    10021 tttgaccttt tccaacatta gacttattca tatattttaa cggcgggtct cataatgatg
    10081 attaactcat cccttcttca ttacaaaaaa ggccgcgagc ttgggagggg ctcacgacca
    10141 tac
//
LOCUS       DAKTIP010000093         9937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAKTIP010000093 DAKTIP010000000
VERSION     DAKTIP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.093"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(72..629)
                     /locus_tag="O0305_001656"
     CDS             complement(72..629)
                     /locus_tag="O0305_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCY0274802.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPYTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(696..2336)
                     /locus_tag="O0305_001657"
     CDS             complement(696..2336)
                     /locus_tag="O0305_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCY0274803.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK"
     gene            2530..2631
                     /locus_tag="O0305_001658"
     CDS             2530..2631
                     /locus_tag="O0305_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274804.1"
                     /translation="MMLQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(2610..4655)
                     /locus_tag="O0305_001659"
     CDS             complement(2610..4655)
                     /locus_tag="O0305_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCY0274805.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            5241..6296
                     /locus_tag="O0305_001660"
     CDS             5241..6296
                     /locus_tag="O0305_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCY0274806.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            6296..7192
                     /gene="murQ"
                     /locus_tag="O0305_001661"
     CDS             6296..7192
                     /gene="murQ"
                     /locus_tag="O0305_001661"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCY0274807.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            7204..8658
                     /locus_tag="O0305_001662"
     CDS             7204..8658
                     /locus_tag="O0305_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0274808.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            8661..9536
                     /locus_tag="O0305_001663"
     CDS             8661..9536
                     /locus_tag="O0305_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0274809.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(9730..>9937)
                     /locus_tag="O0305_001664"
     CDS             complement(9730..>9937)
                     /locus_tag="O0305_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274810.1"
                     /translation="MAVTVGNFTSIFGMFAAILFMYYYLLYKSPEYNQRKRFKHFIHI
                     TNLIIIAFSTFVLVHLALKLFFSI"
BASE COUNT         3213 a         1635 c         1941 g         3148 t
ORIGIN      
        1 acaacccgac gtattaatac tttgtgaaat aaagatcccc ctcaacatga tcgaaggggg
       61 gaagtatatg tttagttcaa tatatttaga aagtcgtttg tagttattgt ttgtcccatc
      121 aatgggaata cattatctat tggaaattga tgtagcgttt cgttttgtgc actcatcata
      181 tctgtaacaa aaaactgatt gtagtttaat tgataggcat ctcgcgctgt cgtatctacg
      241 ccaatatgcg ttgcgacacc accaagaaca atcgtatcaa ttcctcgacg tcgcaattgt
      301 aagtccaaat ctgttcctac aaatgcacta aaatgtcgtt tgtctatgac aaaatcgtca
      361 tctctcttgt ctaataaatg atggaaacga ctgtagtcgt cgccttcttt tggtggtaat
      421 gagatcattg catttggttg caatacatct ttaccatcat agaaattcac gcgaacaaaa
      481 gcgataaagc cattgttttt tctaaaaaca tctattaatt tattagcgtt ttgaacgaca
      541 ttttcagctg tatatggggc ataatccatt ttaagaatac cttcttgcag gtcgattaac
      601 actaaagcgg ttttattaaa attaatcatc ttatatcaga gtcctattcg tttaataaag
      661 tattcgctta aacttatacc ctgtatacga tgaaattatt tattttgttg tgaaaaagct
      721 ttagcgatat cgatgagttt cttcggtgcg tcttcgacag ccattttgac ttcgacaaaa
      781 tgcatcacat cgggatgacc attaattgca ttaaacgtgt cttgtaaatc ttttgatgat
      841 tcaacgtcat gaatttcaac atttttacca ccaaatacag ctggtaaagc tttataatcc
      901 cacatgtgaa tttcattata aggttcatac atgccgtgaa taagtcgttc taccgtatag
      961 ccgtcattat taatcacaaa taataccggt ttaatatgct gtctaatcat agttgaaata
     1021 gcttgaacag ttagttgcaa tgagccatca ccaattaata ataagttacg acgatctttg
     1081 tctgctaatt gtgaacctaa tgttgcaggt aatgtatagc cgatagaacc ccataacggt
     1141 tgccctataa aagtattgtt tttgtataat gctaaatcat aagcaccaaa gaatgatgta
     1201 ccttgatcag caatgatgac atcatttggt tttaagaaat tttgcatcat tttaaaataa
     1261 gtttgttgtg ttaatggttc tgtgccaaca gtataatcgg gtgatgttgg acgatgatac
     1321 gcagggaacg ttgcgttatt cgtatatgaa atattggata actgttttaa caatgatggt
     1381 agagatattt catcatttgt aacatcgtca attttgatat tgtgatgatt taacataacg
     1441 acatcatcga tattgaattg gtatgaaaaa cctgctgttg ctgaatctgt taatttggct
     1501 ccaatattta aaattaaatc gctgttgtcc acataatctc gtattttatc ttcggcaatt
     1561 ttcccatcgt aaatacccat ataatatgga ttttcctcat taaaagcacc ttttcctaat
     1621 gaaagttgtg ctactggtat ctgtgtttga tttacaaaat cttctaattc ttgatggagg
     1681 tgaaaactgt taatttcatg tccagtaatg atgataggct gcttcgcttg atgcagttta
     1741 gttgctaata actctatata tgttgatgca tccgtatatt tagttgccgt cacttcaaat
     1801 ggtgtcggta tctcaatttc agagattgcg acatcgattg gtaaatgtaa atgaactggg
     1861 cgtctttcgg cgattgctgt attaattaaa cgtggtattt cggttgttgc attttcaggt
     1921 gtgatataac cttgtgcaac ggttatatgt gcaaacattt ttcgatagtc gtcaaatgta
     1981 ccttcaccaa gtgagtgatg tacatattta ccgccttgtt caacagcacg tgtcggcgca
     2041 cctgtaatcg caatgacagg tatgcgttca gcatatgaac ctgcgatacc gttgacggca
     2101 cttaattcgc caacaccaaa tgtagtaact aatgcagcga gtccattaag acgggcataa
     2161 ccgtccgctg cgtaacttgc gtttaattca tttgtatttc ctacccaatc tacattggga
     2221 ttgctgataa tatcgtctag aaaagcgaga ttaaaatcac caggaacacc aaaaatttta
     2281 tcgacgcctg ctcgatgaat agcgtcaatt aagtaagctc caatgcgttg tttcataaaa
     2341 aaagccacct tttctctata tttacactta aaatgatagc ctcgaatata gtgtaaggtc
     2401 aaggaagttg actttgttgt gaaaacgcta acaatagtga acaattaata ttttttagga
     2461 aatgagtatg aaggggggct ttaattgatg atcttaaaat tcaaaattta aggggagagc
     2521 gaactatcaa tgatgctgca aaatacaata gcaaagaaaa aacaggcgcg tcaccttatg
     2581 aaggtatacg cacctgttta tagtaagcat tatttagctt caaataattg atcgccaaat
     2641 gaaatgttgc catgttcacc ttgtttaaaa tcaaggtttg taatgtttcc ttgtgtcacg
     2701 ataataggcg taatatcact ctttgcatga ttgcggatgt agtctaaatc aaagttgatt
     2761 aataaatcac cttgtttaac ttcttgacct tcctcaacat gtaaagtaaa gccttctccg
     2821 tttaatttaa cagtgtctaa accgatgtgg attaatagtt ctaaaccact atctgataca
     2881 agaccaattg catgttttgt tgggaaaatc atttgtactt taccgttgaa tggtgcacga
     2941 acttcacctt gtgaaggttt gatagcgata ccgtcaccca tcattttttc gctgaacact
     3001 tgatcaggca cttctgataa tggtgttact tcaccagtta atggtgcatg cacgatatgg
     3061 ctcaattcgc ttgttgcaga tttatcttct gcaacaacaa cagtttcgtc tttatcgtct
     3121 tccatagtag taggattttc tactacttga ccattcataa tctgttgcat ttcatgtttg
     3181 atttggtcag atttaggacc aaaaattgct tgcatattat tgccgacttc taatacacca
     3241 gatgcgccta aatctttcaa accaggaaca tcaactttag atttgtcgtt aacttcaaca
     3301 cgtagacgtg tgatacaagc gtctaaatgt ttaatgtttg ctttgccacc catagcttct
     3361 aatactgcat atggtaattc agttgctgaa gcagtagccg cttgtgattg tttatcttca
     3421 cgacctggtg ttttgtattt taattttaca attaagaatc ggaatacgaa gtagtaaata
     3481 actgcgtata caagacctac aggaatgact aaccaccatt gtgtcttatt aggtagtata
     3541 ccgagtaaga agtagtcgat gaaaccacct gagaatgtat aacctagatg aagatctaat
     3601 aagtacaatg ttaagaatga taaaccatca agtactgcgt gaataaagaa taataatggt
     3661 gctacaaata agaatgagaa ttctaatggt tctgtaatac cagttaagaa tgatgttaaa
     3721 gcagcagaac ccattaaacc tgctactact ttcttatttt caggtttagc tgtgtgataa
     3781 attgctaaag ctgctgcagg taaaccgaac atcataacag ggaattcacc ttgcatgaat
     3841 ttaccagctg tcaaatgtgc gccttcacga atttgttcga taaagatacg ttggtcaccg
     3901 tgaataattt caccagctgc atttttccat gaaccaaact cgaaccagaa cggtgcgtgg
     3961 aaaatgtgat gtagaccgaa tggaattaat aaacgcttga tgaaaccaaa taagaatacg
     4021 gcaacaccag tatttgaatc taataatcct gtactgaatg catttaatcc tgattgaatc
     4081 gttggccaaa ttaatgccat tgggaatgct aaaataaatg atgttgtagc catcataata
     4141 ggtacgaaac gcttaccagc gaagaaacct aaataagatg gtaagttaat gttatagaac
     4201 ttgttataac accaagctgc cagggccccg attataatac cgccgaacac acctgtttgt
     4261 aatgttggga tacctaaaat gctagcgtaa ccactcgctg gatcaccaat attcttaggt
     4321 gtaacttgta aaaagtcgcc cattgttttg ttcatgatta tgtaaccgac gaatgctgcg
     4381 atagctgcta cgccatcacc gccagctaat ccgattgcga cacctaatgc gaaaatcata
     4441 ggcaagttat caaaaatgat accaccagca cctgtcatta atttagcgac agtttgtacg
     4501 ccaccatttt gtataaacgg caagtagtgt tgtaatgatt caccttgcat agctgtaccg
     4561 atagctaata acagaccagc tgctggtaaa atcgcaacag gtaacattag cgctttacca
     4621 atacgttgca attgaccgaa aagtttcttc ctcacttgtc caacctccaa agttgtattt
     4681 attatttaag attcaataaa aaaagacacg agcaaataga ctgtgttggt aaccatcaca
     4741 gcttaattta actcatgcct aatcttactt agtaacacgt tggtgtatgt aattaatgta
     4801 aaagaagcaa ttgactaagt agtatataaa actgatacgt tagtttatct agcttaccat
     4861 cacatcttat tgaatcttat ttgtttgcga ctcctatttt agcatggtga agtatgcgtt
     4921 ttcaatacaa ttattaaatt caatttcaaa tctcttaatt gttcacagat tattattaaa
     4981 acataatata agataatcct gaataaaatg tataaaagtt attgtaataa tccagcagta
     5041 gcagctatta tcagcactta aaagaaagga taatctttga tggtgtaata attaacttgt
     5101 gacagaaata tgtaagatta ttacaatgaa caggctttaa ctgattaata ttgtaatgtg
     5161 ttttctctta tagtaaaatg taagcgatta cacaactcaa ctatattttc ctaataatta
     5221 tattgttaag gagggctact ttgacaggct tttcagtgta tttaggacaa cctttagatg
     5281 aagcgtatat taagcgaatg attaaacaag gttaccaaat gatttttaca tctgtacaaa
     5341 taccagaaga agatgacgag acaaaatatc attatttcac aaaactactc aatttattaa
     5401 aacatgaaca agtgacttac ctcatagatg ctaatccatc tattttaaca ccatcttttt
     5461 atgaccatct tcgacaatat gatgcacaat ttatgattcg tatcgatcat agtacatcaa
     5521 ttgaggcaat cgaagcgata atggcacagg gtttaaagtg ctgtttgaat gcaagtatta
     5581 tttcccggga attgttaaca agcttacatc aacaattgaa tgattttaca ttactttcat
     5641 tttgtcataa ctattatcca agaccagata cgggattatc tgttgacttg gtcaataaga
     5701 aaaatgaact catttatcaa tttaatccaa aggcacaaat atatggtttt attgtaggga
     5761 gtgatttgcg aggtcctttg cataaaggct tgccaacaat tgaagcaacg agacatagtc
     5821 atcctgtcgt tgcagctaaa ttattacaag aaactggtgt atctgaagtg ttagttggag
     5881 actcattgat tgaaatgagg caggcaaaac aacttataga tttttgcaag cataggcatt
     5941 tcacgttatg tattgaagaa gtgtttgata cgacagtgac ttaccttttc gatatgtgtc
     6001 ataaagtacg cccggataat ccggaaaatg tcattcgttc ggaaacgtca agacaaatat
     6061 gtccacattc gattcaacca cagtttacga cgcaacgacg cattggttca gtaaccgttg
     6121 ataatttgaa taacggacgt tatcaaggcg aaatgcaaat tgtgagacaa acgcttagtg
     6181 cacatgacaa tgtgaatgtt gttgcacaaa ttattaaaga agacttacca ctgttaagtt
     6241 gtatcgagcc gaatgataca tttgattttc aaaaaactag ggagtgtaag aagtgatgga
     6301 aaatagtacg accgaagcgc gtaatgaagc gacgatgcat cttgatgaaa tgactgtgga
     6361 agaggcttta attacgatga ataaagaaga tcagcaagtc ccgttagcag ttcgaaaggc
     6421 aataccacaa ttgacaaaag taattaaaaa aacaattgca cagtataaaa agggtggacg
     6481 attgatttat atcggtgcag gtacaagtgg aaggttgggt gtcttagatg cagcggagtg
     6541 tgtacctaca ttcaatactg accctcatga aattataggt attattgctg gtggacaaca
     6601 tgctatgacg atggctgtag aaggtgcgga agatcacaaa aaattagcgg aagaagattt
     6661 gaaaaatata gatttaacat caaaagatgt cgttatagga attgccgcga gtggcaaaac
     6721 gccatatgtt ataggcggtt taacatttgc taacacaatc ggtgctacaa cagtatctat
     6781 ttcatgcaat gaacatgcag ttataagtga aattgcgcag tatccagtag aagttaaagt
     6841 tggtccagaa gtattaactg gttcaacacg tttaaagtct ggtacagcac aaaagttaat
     6901 tttaaatatg atttcaacca tcacaatggt tggtgtcgga aaagtttacg ataacctcat
     6961 gattgatgtt aaagcaacca atcaaaaact gatcgaccgt tcagtgcgta ttattcaaga
     7021 aatatgtgct atcacatatg atgaagcaat ggcgttatat caggtatctg agcatgatgt
     7081 gaaagttgcg acagttatgg gtatgtgtgg catttctaag gaagaagcaa caagacggtt
     7141 attaaacaat ggtgacattg ttaaacgagc aatcagagat agacaacctt aggagggatt
     7201 taaatgacca aagaacaaca acttgcagaa cgaattattg ctgcagtagg tggtatggat
     7261 aatatagata gtgtcatgaa ctgtatgaca cgtgtgcgta ttaaagtatt agatgagaat
     7321 aaagtagatg accaagaact aaggcatatt gatggtgtca tgggtgttat acacgatgaa
     7381 cgcattcaag ttgtggttgg acctggtaca gtcaataaag tggctaatca tatggcggaa
     7441 ttaagtggtg ttaaactagg tgacccaata ccacaccatc acaatgatag tgaaaaaatg
     7501 gactataaat catatgcagc tgataaagca aaggcgaata aggaagcgca taaagcaaaa
     7561 caaaagaatg gtaagttgaa taaagtattg aaatcaattg ccaatatctt tataccgttg
     7621 attcctgcat ttattggagc tggattaatt ggtggtattg cagcagtact gagtaactta
     7681 atggtggcag gctatatttc aggtgcttgg attacgcaac ttataacagt atttaatgtc
     7741 attaaagacg gtatgttagc atacttagct attttcactg gtattaatgc ggctaaagaa
     7801 tttggtgcga caccaggact tggtggcgtg attggtggta caacgttatt aacgggtatt
     7861 gctggtaaaa atattttaat gaatgtcttc actggagaac cattgcaacc tggacaaggt
     7921 gggattattg gcgttatttt tgccgtttgg attttaagta ttgtcgaaaa gagattacat
     7981 aaaattgtgc caaatgcgat tgatattatt gtaacgccga ctattgcatt gttgattgta
     8041 ggactattaa ctatctttat ctttatgcca ttagcaggtt ttgtttcaga cagtttagtt
     8101 tcagtagtta acggaattat tagtattggt ggcgtattta gtggatttat cattggtgca
     8161 agcttcctac cgttagttat gttagggctt catcatattt ttacgccaat tcatatagaa
     8221 atgattaacc aatcaggtgc tacttactta ttgccaattg cagcgatggc tggtgctgga
     8281 caagtaggtg ccgcattagc actttgggta agatgtaaac gcaacacaac attacgtaat
     8341 actttaaaag gtgcattgcc agttggtttc ctaggtatcg gagaaccatt aatctatggt
     8401 gtgactttgc cattaggtcg acctttctta actgcttgta ttggtggtgg tattggtggc
     8461 gctgtaatag gtggaattgg acatattggt gccaaagcaa taggcccaag tggtgtgtca
     8521 ctattaccat taatctcaga taatatgtat ttaggttata ttgcaggatt acttgctgcg
     8581 tatgctggtg gattcgtttg tacatattta tttggaacga caaaggcgat gcgacagaca
     8641 gatttgttgg gtgattaatg atgacaaata ttttatatcg cattgataag cagttgagtg
     8701 attttacgaa gacagaaaag ataatcgctg attacatttt aaagaatcca cataaaatca
     8761 ttgatatgac tgtgaatgat ttggcagatg ttacgaatgt tagtacagca tcaattgtta
     8821 gatttagtcg gaaaatgaca catcaaggtt ttcaagagct aaagattgcg atatctcgat
     8881 acttacccga agatattgca accaatccac atttagaatt gattgaaaat gaatctgtag
     8941 aaactttgaa aaataaaatg attgctagag caacgaatac gatgcgattt gtagctacta
     9001 atattatgga tgcgcaaatt gatgcaattt gtgatgtgtt gaaaaatgcc aggacaatat
     9061 ttttatttgg atttggcgca tcgagtttga ctattggtga tctttttcaa aagttatctc
     9121 gtattggctt aaatgtcagg ttattacatg aaacgcattt acttgtgtca acatttgcga
     9181 cgcatgatga tagagattgc atgatttttg tgacgaatca aggtagtcat agtgaattgc
     9241 agtcaattgc acaggtggcc acacattaca gtattcccat cataactata tctagtacag
     9301 ctaataatcc agtggctcaa attgcagact atgcattgat ttatggcaga actgatgaaa
     9361 atgaaatgcg tatggcggct acaacgtcac tatttgcaca gttattcacg gtagatatat
     9421 tgtactatcg atttgtagca ttaaattatc atgcgattct agattgtata acccaatcga
     9481 aaatggcact tgataattac aggaagcatc ttgcgacgat agattttaaa cattagatat
     9541 tgtgtgcgtt ctaacataaa gttagttgca acgtaaacaa ttgaagtttt tgatgtgatt
     9601 cagttgcttg atgaaaaatt gttgctacaa gtttgtaaaa ctggcataga ggctattttg
     9661 aaaatgataa aggcgtgaca aattaaagca aagttcagat ttgagctttg cttttttcat
     9721 agatggaaat taaatgctga agaataattt taatgctaaa tgaactaata cgaaggtgct
     9781 aaaagcaatt attatcaaat tagtgatatg aatgaaatgt ttaaatcgtt tgcgttgatt
     9841 gtattcggga cttttataga gtaggtagta gtacataaag agtatggctg cgaacatgcc
     9901 gaatattgaa gtgaagttac ctacagtgac tgccatg
//
LOCUS       DAKTIP010000094         9856 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAKTIP010000094 DAKTIP010000000
VERSION     DAKTIP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.094"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(319..1878)
                     /gene="rny"
                     /locus_tag="O0305_001665"
     CDS             complement(319..1878)
                     /gene="rny"
                     /locus_tag="O0305_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCY0274811.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2232..3275)
                     /gene="recA"
                     /locus_tag="O0305_001666"
     CDS             complement(2232..3275)
                     /gene="recA"
                     /locus_tag="O0305_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCY0274812.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
     gene            complement(3440..4591)
                     /locus_tag="O0305_001667"
     CDS             complement(3440..4591)
                     /locus_tag="O0305_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCY0274813.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLTNHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            complement(4818..5396)
                     /gene="pgsA"
                     /locus_tag="O0305_001668"
     CDS             complement(4818..5396)
                     /gene="pgsA"
                     /locus_tag="O0305_001668"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCY0274814.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            complement(5430..5822)
                     /locus_tag="O0305_001669"
     CDS             complement(5430..5822)
                     /locus_tag="O0305_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274815.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            complement(5841..6668)
                     /locus_tag="O0305_001670"
     CDS             complement(5841..6668)
                     /locus_tag="O0305_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCY0274816.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            complement(6773..7477)
                     /locus_tag="O0305_001671"
     CDS             complement(6773..7477)
                     /locus_tag="O0305_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0274817.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            complement(7477..8763)
                     /locus_tag="O0305_001672"
     CDS             complement(7477..8763)
                     /locus_tag="O0305_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0274818.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            complement(8763..>9856)
                     /locus_tag="O0305_001673"
     CDS             complement(8763..>9856)
                     /locus_tag="O0305_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0274819.1"
                     /translation="SFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGDK"
BASE COUNT         3070 a         1872 c         1281 g         3633 t
ORIGIN      
        1 aacagattta caattaaaat aactcaactt agaaataaat cctaattaat attgcagaag
       61 agaagatgtg ttacatcttc tcttcttttt tattggatag aaaagcaatc atctttatag
      121 gtttacataa tataaatata gtaattacga agcctaaaac atttttatta aaagcaataa
      181 cttcttattt aatataaggt ccgtcttact ttcaaataac tactgtttaa tatagttatt
      241 accgataaag tgctgtctaa ctagattaag actacaactt aaaaaagctc cctcactaat
      301 ttgtgaggga gacaaaaatt atttcgcata ttctactgct ctagtctctc gaacaactgt
      361 caccttgata tgaccaggat attgtaattc atcttcaatc tgatttttaa tatctctagc
      421 caatcgataa gattttaaat catcaatttc ttcaggagat acaatcactc ggatttctct
      481 acctgcctga atcgcaaatg ctttttctac accatcataa ctttctgata acgtttctaa
      541 acgttctaat cgacgaatat aattctctaa tgtttctttt cttgcacctg gacgagccgc
      601 agacaatgca tctgcagcag caacaaggat agatataata gatgtaggtt caacatcacc
      661 atgatgagaa tggattgcat taataactgt ttcattttca ccatattttt tcgctaattc
      721 tacaccgatt tcaacatgac taccttctac ttcatgatca attgctttac caacatcatg
      781 taaaagtcca gctcgtttcg ctaatgtctc atcttcgcct agctcagcag ctaacatact
      841 agcaagatgc gcaacttcaa ttgaatgttt aagtacattt tgaccgtaac tcgtacgata
      901 gtttaaacgc cctacaattt ttactaagtc aggatgcata ttatgtgcgt tcacttcaaa
      961 tgtagcttgt tcacctgctt ctctaataat atcgtctact tcttttctag ctttttcgac
     1021 catatcttca attctacctg gatgaatacg tccatcagat actaagttaa caagtgctgt
     1081 tctagcaatt tctcttctta ttggatcaaa accagataat ataaccgctt ctggtgtgtc
     1141 atcaataatt aaatcaatgc cagttaaagt ttcaagtgtg cggatgtttc gtccttctcg
     1201 tccaatgatt cgacctttca tctcatcatt aggtaagtta actactgata ccgttgattc
     1261 acttgtgtga tctgctgcta atctttgtac tgctgtagct aataattctt ttgctgtttt
     1321 atcaactttt tctttagctt ctttttcttt ttctttaaca agtactgcaa tatcttgtga
     1381 cagttcttcc tctactcttt gaagttgctc attaatagct tcttcttgag tgagaccgga
     1441 gatgcgttct aattcttgtt catgcttcat tattaacgtt tgaacactac tctcttttgc
     1501 atctacttgt tgttgttttt cttcaatttt tgattctttt tgctctaaaa tctcatcttt
     1561 tttatctaat agatcagatt tgcgctctaa gttttcttct ttttgaagaa gtcgggtttc
     1621 ttgtctttga agttcgctac gtctttctcg tagttctgct tcagtttgtt ctcttaggat
     1681 ttggttttct tcttttgcct caagtaattt ctcttttttg atattgtcag cttctttatg
     1741 tgcttgattt acaatatctt cggcagtttg tctagcttgt gattgctttt gaagcaacaa
     1801 atttcgggca acaacatacc ctccaacaac tcctagaatg atccccagca aaatgagtag
     1861 gaggcttaat aaattcacac aaacacctcc ttttctaggg tttgctcttt atatgaaatt
     1921 caatatgact ttatgaaatt atatataaat catacaaatt aattgtacgg tttataggag
     1981 ataagtgtca agttatcaat ttgcactttt gcactatatt gaatgctatt tctactattt
     2041 tctaaagttt tgaagctcga ttataaaact attgataatg taataataaa taaaataaaa
     2101 aaccttatag tctcgatttg tagtgtatcc cataaagtta gatattattc tagctttatg
     2161 agatgaatat cactttcaat caatcctata aggatatttg ctaagtttaa ctatagatat
     2221 aaatttgtgt actattcttc gtcaaataat gactttggtg catcttctgt ttcttcaaca
     2281 tcaccatcag atatacctaa tttttctctc aatttacgat caatttcttc tttaatttgt
     2341 ggattttctt tcaagtacat tttaacattt tccttacctt gacccattcg ttcgccattg
     2401 taagaatacc atgctcctga tttatcaacg atgtcgtttt caacacctaa atcaataagt
     2461 tcaccctctt tagaaatacc ttgtccatac ataatatcaa cttcagctac tctaaatggt
     2521 ggtgccactt tatttttaac gactttaatt ttagttctat tacctacaat ttcttgtcct
     2581 tgtttaagct gttctgcacg acgtacttct agtcttactg aactatagaa ttttaatgca
     2641 cgtccacctg gtgtagtctc tggattaccg aacataacac caactttttc acgaatttgg
     2701 ttgatgaaaa tagcagttgt atttgattta gaaatagcac ctgaaagttt acgtaacgct
     2761 tgtgacatta aacgagcttg taaaccaacg tgagtgtctc ccatttctcc ttcaatttca
     2821 gctttaggtg ttaaagcagc aactgagtct acaactacaa tatcaactgc accacttcta
     2881 acaaatgctt cggcgatttc aagaccttgt tcaccatgat ccggttgcga taaatataaa
     2941 ttatcgatat ctacgcctaa tgcttgagca tattctggat ctaaagcatg ttcagcatcg
     3001 ataaatgctg ccaccccgcc attactttgt acttcagcaa tagcgtgaag cgctactgtt
     3061 gtcttaccag aactttcagg accataaatt tcaataattc gtcctttagg atagccacct
     3121 acacctagcg cattatctaa tgtaactgaa ccagttgatg tagttgaaac tcggcgacct
     3181 atattgtcac ccaacttcat tacggcacct ttaccgaaag atttctccat atttttaatt
     3241 actgtatcta aagctttttg acgatcgtta tccaaagcga gacctcctaa ttgaaattgc
     3301 tatcttttaa ttatcttacc aatactatac aaggattcaa aataaaatac aagtgttttg
     3361 cgaatatttg ttcgtataaa atcttgaaat caaaacgcaa aaacgaacaa acgtgcttat
     3421 atttttatcg ataatacaat taatttgttg ttctaagcca atttaataat cttatgagcg
     3481 cataattttg acttctgttc tttaataaat ttctagattt agacatttta aattgctcag
     3541 ttttaaattc aaagttatta tcatagccta tatgaactac accatcatac tctaatagaa
     3601 taattgctga tgacacatta aacaatttat taacaaattg cactgcatct tttaattgct
     3661 gctcaatagg tttattaata tcaacaaagt tttcattgtg attaatcata ccctttagaa
     3721 catcgtttaa atccacttct ttcaatcgat gatataaagc accattagta ataccatcat
     3781 atattacaaa aggttcatga attttcttta ttacagcttg ctcaattaat gtgtcatctg
     3841 aaccataata atattctcca atacgatcaa gaatttcttg tttaacaggt tgaatcaatg
     3901 attgtgcttg ttcttttgag tcggcatttg cagtcaatct aatatatact tcatgacttc
     3961 ccgccaaagg cgcaatcgta ggattagtct gtttatcgat aagatctatt aatattgttt
     4021 ctactttaga ttcacctatt cccgcaaatc ttaatagttc agaatgtata attcgattat
     4081 ggtttataaa atgtgacaac aattcatttt tcaccattgg ttgcatttct ttcggtggac
     4141 ctggtaataa aataatttgt ttgttttcaa aattcaccat cattcctgga gccatgccat
     4201 gatgatttgt taatacagtt gaaccttcaa ttactaaagc ctgttgttta ttattaggtg
     4261 tcatttcttg tccttgttcc tcaaaatagc tttcaatata ttttaaagaa ggctcatcaa
     4321 taactaaatc tttaccaaca atctgggcca ctgtatgctt cgttaagtca tctttcgtag
     4381 gacctaagcc acctgttaaa ataacagtat catatttttc tagcgcatga cgtacacttg
     4441 attctaaacg ttttttatta tctccaataa ctttatgttc taatacattt tgtccaattt
     4501 cattaaatac tttagataga aattgtccgt tggtattagc gatttgacct agcaatagtt
     4561 ctgagcctac agcaataatg gcaattgaca tttatatccc ccttgtatcg tttaattcat
     4621 tttttcatgt atttcattta aaaattcaca tcaattttag attcaatgta tcagatgcta
     4681 ctacagattt tccctaaaaa aatgaacaaa tattgtttta caatatatca ttataaacaa
     4741 ttgcgtggct aatttctaaa attgggatta aaaaaactag attccatatg aaaatgaaat
     4801 ctagtataaa caaatattta tttttgttta aaaacatctc tacctttata aaagtattca
     4861 ataccagata agatagtaaa aataacgcca atgtataata aaatttgtcc taatgacaaa
     4921 ccaatcaatg ttgccaatgg atcacctaat aacaaccaag taattgctac catagtaact
     4981 gctgttttaa ttttacctaa ttgaccagct gcacttacga atccttgttc aatttgtagt
     5041 aaacgtaaac cagttacggc aaattctctg gcaataataa tgattgctac tacagaattt
     5101 gttagtccta gttgcacaag tacaattaaa gcacttgcaa ctaataattt atccgctaat
     5161 ggatccaaaa atttccccat atttgtaact aaattccatt ttctagctaa ataaccatca
     5221 acaaaatcgc taagggaagc caatataaaa ataaaaccac tgattaataa ctcaattctt
     5281 atttcatatc ctcctagaaa tgacacattg ccaaatccaa aatcaactaa cgcaaacaat
     5341 ataaaaactg gtattaacac tactctaaaa accgtaatct ggttcggaat attcattata
     5401 catcctcatt tctcactaat ttatttctgt taaaatatta aaactaacat gatccataac
     5461 aataaagtta ttaaaactgt aatacccatg attactaata attgtattgg ctcttttgat
     5521 ttactcttat aatctaagtc tttattatta ttgaaaactg taattacttc gtcccattcg
     5581 gcttggttcg atggaatttc atcttgatga gcttgaatta attggttagg ttcaatattt
     5641 actacgcttg catattttct aataaatcct tcgctgtaat ttttattcgg tagttgatcg
     5701 aattcattat tttcaatatg cactagcatt tcacgtttaa ttccagtacg ttgctctaat
     5761 tctgttaaag tcattcctaa cctttcacgt ctacctttta gcgcttcacc gaccgttttc
     5821 aattcatagc ctccttacac ttactcgaag aaaccaaaac caccaccaaa cggatctcca
     5881 aaatctaaat tattcttact ttggactgtc tgtttcttca tttcttcgta atgtatttct
     5941 tgattttcat tttctctcaa ttcgataata tagtcaaaat cttccattgt acaagtactt
     6001 gtttcaacaa ataaatctgg atgttcaacg actttgattg atggtaacgt cataacttcg
     6061 ttaacaagct cttgatgttt tggattagat tctctggcag taactgcacc atcaataatg
     6121 tatacatgat tggcatcata ttccccttta attagagagc tacgtacagt ttgttttatt
     6181 aaagtagaac taataaataa ccatctctta tgtgcacaaa cacttcccgc aacaattgat
     6241 tcagttttac caacccgtgg catacctcta atgccaatca acttatgacc ttcttctttg
     6301 aacaattcag ctaaaaagtc tactaacaag cctaaatctt cacgctcaaa tcgaaaggtt
     6361 ttcttatctt ttgcatcttg ctcaatatat cttccatgtc ttactgcaag acggtctctt
     6421 aattctggtt ttttaagctt tgttatttca atttcattta taccacgagc tatttgctca
     6481 aaacgttcaa ctttttcaag attgtctgtt ttaattaaaa ggcctcgttt accttgatca
     6541 acaccattaa ttgtaacaat acttatacct aacataccta ataaactaga tacatcacct
     6601 aaaagacccg gtctattaat ttcaatttcg tattctaggt accattcttt tttctctgca
     6661 actgtcatgg tgaacctcac cccttgttta attaacaaat tatgattcat tgtaatattc
     6721 atattataac atcaatcata aaaacatttg ctcataaaaa tagttctata ttttaaatat
     6781 accaagcacc attaactttc tgtatagttc cagtaacact tctagcattt gggtgatata
     6841 aataggcgca tgtatgagca acctcactcg gtaaaaccaa tcgctgttgt ggtaattcag
     6901 ttatcattgc ttgaagttca tcttcttgcc actcacttgc catattaccg gctacaaatc
     6961 caggtgcgat agcatttact gtcactgatg ttagtgcaag ctcttgacta agcgccttaa
     7021 cgaaacctaa ttgtgcactt ttcatcgccg aataaatagt ttccatacta gctcctgtct
     7081 caccccaaat tgatgaaatt acaataattc ttccattggc actttgacgt aaaacatcaa
     7141 cgaaatatct acataatcga attaattgca agacatttaa ctgataacat gcatcaatat
     7201 catggtcttt catatcttgt aaaacaccat ataaagactg accacttgca tatattaaac
     7261 agtctaatga tttaatgtca ccaaatgttt tgtcaatatc aattgtttga gataaatccg
     7321 cttgtataaa acgaacttta tcatcattaa atttgctagt taattcattt atatcagtac
     7381 gataatattg cacataaact tcaaatccat cagttaataa ttgtttgact atctcagaac
     7441 caattgaacc agaaccacct aatactaatg ctttcattac tttttaatct ccaaacgact
     7501 atcgacttgc tgatctaaat ttaaatataa cgatgacgtt tcgttaatac tatctaacgt
     7561 gatattttca acaatgtcta acatgtcaaa cacgctaaca ccttcaaaat acaatttagt
     7621 atattgatta gcaatatatt caggtgagtt taaacttgat atgaattcac ctataaattg
     7681 ctttttcaaa agttcaaatg cttctgcatc ttggaaattg ccttttttat cacgcaactc
     7741 atctaataat aattttttta atttatctgg ttcttcagta gcacttgtca cgattgaaaa
     7801 actatacgtc ggctctagta caaattgata accaaatgta tcatcgataa gtccttcgtt
     7861 taataaattc tgataaaaat ctgtttcttc cccaaaaatt aactcaaaga ataatgacat
     7921 ttctaaatca cgttgtacat atttttgagg cgcttcttgt aatggtttat ttttaaaacc
     7981 aagcattagt cttggtgatt gaattttcat agattcagta acaaatgctt ctttaacatc
     8041 ctccggttca tcaacaagtc ctcgttcgat tttgggttgg ttaactttat tacgagcatc
     8101 ctcgtgttgt tttactattc gacatattgc ttcaggatcc acatcgccaa caacaaataa
     8161 aaccatattt gatggatgat aaaacgtttc ataacataga tacaaatcat cttttgtaat
     8221 atcgtatata ctttctacac taccggcaat atcaacacgt attggatgtt gttgatacat
     8281 tgcacgcaat gtatcaaaca ttaatttata tccaggttgc tcttgataca tttttatttc
     8341 ttctgcaata ataccttttt ctttatcaac agtttctttt gtaaaataag gcgtttcaac
     8401 cattgtaagt aaacgtttaa tgttgttttc aatattatca gttgcactga acaagtagct
     8461 tgtacgatca aagcttgtaa acgcatttgc ttgtgcgtta tcttcagcaa acgcagtaaa
     8521 taagtcttct tcttcttttt caaataattt atgttctaaa aagtgagcaa ctccatcagg
     8581 tacagtaaca aattggtctt gtccaagggg tttgaattga ttatctaatg aaccaaattg
     8641 tgtagtgtaa gtgacaaatg tcttttgaaa acctggtttg gggataataa ataatcgtaa
     8701 accattttct aattcttgtt cgaatactct ttcgtctatt aattcataat aacgctcttt
     8761 cattatttat cccctccttt tgtcaacaca tagattgtat ctaaaaatgc tttttcagca
     8821 acagaaacaa tatcttcgcg acttaccttc tgtatatcat ttataaatgt ttctttgctt
     8881 tgtggttgct ctaataatat ttggttatgc ataatctcta taatactttt cggacgatct
     8941 tcagattcat atcgatgaga aatgattact tttttagcta actctaattt ttcttcagtg
     9001 aaatctcctg cttttatttt ttcaaattca cttataatag tgtcttttgc agtttcgtac
     9061 ttatcacttg aaaccccact caaaacaaat aaatagccat ttttgccatc aatttgtgaa
     9121 tgtatagagt acgctaaact ttgcttttct cgcacttcat taaataaaac agatgaagga
     9181 tctcctccaa acatcatgtt aaatacaaca aaggcagcat atccactttg tccatattgt
     9241 gttggaaaac ggtatcccat atttaattta gcttgatcca cgtcatcata ttcaacaata
     9301 taatcaactt cttcatcgtg taaatgatga gtagaatgtt ggaattgatg tttatcgaat
     9361 ggtttaagtg caaatttttc acgtatttgt ttctcaacac tttcaggttc tacattgccg
     9421 acaacataaa cagaacattg atcattatta atcattgatt gatatgtatg atatagtgtt
     9481 tcagcagtaa tatgtgggat ttgttctagt tgtcctgtag ataagtattt atatgcttca
     9541 ttttcaaaca tatggtcgag taattttaaa aacgaatatt gtgctttatt atctaccatt
     9601 gcttctattt ttttggctaa taatgttttc tcttggttaa caaaattatc attgaatgct
     9661 ttattttcaa ttaatggatt ccaaatgatt tcttgtaata aatctaatcc ttgattaaat
     9721 aatgattcac cgtttcttaa ataacgttca ttaacaattt ctaatgaaaa tgtaatgaca
     9781 tgctgatctt tgaattttga aattgtacta ttcacatacg caccatataa atcggctaag
     9841 tgattattaa acgact
//
LOCUS       DAKTIP010000095         9769 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAKTIP010000095 DAKTIP010000000
VERSION     DAKTIP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.095"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..845)
                     /locus_tag="O0305_001674"
     CDS             complement(<1..845)
                     /locus_tag="O0305_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCY0274820.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGEPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHEL"
     gene            complement(865..4305)
                     /locus_tag="O0305_001675"
     CDS             complement(865..4305)
                     /locus_tag="O0305_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCY0274821.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEGLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNAQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQALKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLELLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4609..5949
                     /locus_tag="O0305_001676"
     CDS             4609..5949
                     /locus_tag="O0305_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCY0274822.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGAL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(6155..6487)
                     /locus_tag="O0305_001677"
     CDS             complement(6155..6487)
                     /locus_tag="O0305_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCY0274823.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(6571..7716)
                     /locus_tag="O0305_001678"
     CDS             complement(6571..7716)
                     /locus_tag="O0305_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCY0274824.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(7925..8304)
                     /gene="rnpB"
                     /locus_tag="O0305_001679"
     ncRNA           complement(7925..8304)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0305_001679"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(8367..8711)
                     /gene="gpsB"
                     /locus_tag="O0305_001680"
     CDS             complement(8367..8711)
                     /gene="gpsB"
                     /locus_tag="O0305_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCY0274825.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
     gene            complement(8725..9288)
                     /locus_tag="O0305_001681"
     CDS             complement(8725..9288)
                     /locus_tag="O0305_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCY0274826.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            complement(9281..9631)
                     /locus_tag="O0305_001682"
     CDS             complement(9281..9631)
                     /locus_tag="O0305_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCY0274827.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     ILNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTDG"
BASE COUNT         3012 a         1696 c         1393 g         3668 t
ORIGIN      
        1 tgtaattcat gttcgttaca aattgataaa atatgattaa gcgttgttgc aaatgacatt
       61 ttctcaaaaa gcgcttcact atcaattggt acttgtgtgt gaatttcagc cagtcgcttc
      121 gataaatata gttcatctaa attgtcatta atcttattac gttgcccagc tgataatgca
      181 tcaatgtttt caaccacatt ttcaacgctt tgatattgct gaattaactt aatagccgtt
      241 ttctcgccaa tacctttaac acccgcatat ccatctgctg tatcacccat aaacgcttta
      301 atatcgatta gttgttgtgg ttcaagggca tattcttcgt taaaacgatg taatgtatat
      361 ctattataaa tgttaaaacc ttttttaatt agccaaactt caacattgtc attaatacat
      421 tgcagtaaat ctttgtcgcc cgtaataata tagacatcgt tatcagttga atattgttgt
      481 gctaatgtac ctataacatc atccgcttca tagtttttaa cgccaatatt tacaaagcca
      541 aattgctctg aaatttcttt aacataatca aattgtggta tcaattcttc tggtggtgca
      601 gaacgatttt gcttataacc atcaaacata tcatttctaa aagttgattg tcccatatcc
      661 caacatacag ctacatgtgt agggcgtatt tcatgtattg ccgaaaagat atgacgcaca
      721 aatccttgta ttccatttgt aggttcacct tgtgaattgt acataaattg tttatgaaga
      781 cttgtagcat agaaatgtct aaataatagc gccataccat ctacaagtaa tattttatta
      841 ggcatttaaa acgttccttc tttcttaata taaaatagtt ttcagttgtt gatgtttatc
      901 tttcaattga ttgataagtg ttgtatctaa atcaaatgct aataaatcgt ttatttgcgt
      961 ttgaatatta gcttcaaaac tttcaaattg cgattcagct tctacagcca tttgctttac
     1021 atataattca agctgttgcc tcaataatcc catttgtggt tgtaataatt ctaacgtact
     1081 ttgacaaatt gattcatgta tatctctttg cccatttgga ttcaaaattt tacgttttgt
     1141 taattgttta ggcaatgcat ttagcatatc gttgaaatcg atgtgcaata atggttgctc
     1201 tatattagct gattcaaagt taaactgagg attaataatg acatgtaaat ctgctaattg
     1261 ttgaacgatt ggtgcaattt gctcagtgag ttgcttatta aagtattttt ttatacgttc
     1321 tgtaattaaa gattgctcta aaaacaatcg ttgatgaatt tgatctaagt atactctcgt
     1381 agacactttc ttttcttcat taaaatcact attttgcgtc atttgagaat taaacactga
     1441 tttaacatcg tcaagtagtt gtagttttaa acgggcattt aaatgataaa cttgttcttc
     1501 aacttcgttg tctgtatgtt gtaacgtcgc atcaattaat tgatgttgta aacgctgttt
     1561 atctttataa tctgttaacc tttgttggcg acgtgaaata tcagctttat ttgtttcaaa
     1621 ttctgtaatc atctctacat aagaacgatc catttgttga agctgatgaa tcatttgttg
     1681 ttctaaaatt gatttagatt caacatcaac aaattgttgt atgctttgtt ttaattgatc
     1741 aatgccctta tcttcagctt gcaatgcatt tcgacttgat acagcaaaaa tgtctgattg
     1801 taagtgtact tgtcttaatg catctgatac atatgtttca actgcttcaa gatcatcttg
     1861 actttctgct aaatcagcag cattaattac catttttaat gcttggtttt cattcaactg
     1921 gttcatatct ttcatgtgtt ctataaacgc tttgtcatta tcagtaaatg aatgattaaa
     1981 ataacttaca tacaatatta agtctgaaga agttaaaatt tgttcggttt catttgtatg
     2041 cctttggtta tttgagtgta gccctaatga atcaacaatt attttacctt ttaaccaatc
     2101 atgcattaat gcaatgtgta ctgtcttaac aaatgttgca tattcatctt ctgcactcca
     2161 ctttttcaat tcctgttgat taatggcatg tttttctcca ttttccaaca tattgacata
     2221 caatttataa tgtttttcaa ctgcctgaat aaaagcgagt tgatttttat ttaaatgtga
     2281 ttttaacttt tctaaatctg aattaataaa gtcttctata gtcgaataag acatatcttg
     2341 gtattcaact actgcattaa tttcatctaa taattgcgat tgtgatttta gcgttatata
     2401 actctcgtct ccataagata tttctgtagt agctgctgta gtaggatttg gagaactgac
     2461 taaaatatgc tcgcctaata atgcatttat caaactactt ttaccagcac taaatgttcc
     2521 aaatacacct atttttatta atttattatc tatacgtgtt aatgtttcgt ggatatcttg
     2581 ctttgtacgc ttgaacaaag gcacatccga aattatatca agcgcttttt gaatatctat
     2641 agacatattt gttgtagctg tattttgatt tagctgctga ttatcacgat tatcctgagc
     2701 attatcagta gccacttgat atgttgtctc ttgtcgacct attaatttat ctagagattc
     2761 atctaaatga atatagtagt gacgataatt cttagtcgtc aatgactgac gcagctcatt
     2821 taattctgta taacgttgat attcttttaa atcatcactt tcttctgttg gtaattcatc
     2881 tgcctgcaca ttttctatta tttctttaaa aattggtgtt gattgttgtt caacatattt
     2941 cttaatggct ttaaccactt cgtctgaaaa tgtaagtaca taagtattgc tgattgatgt
     3001 ttgtggttga tataaacctt caatcatttc tggcttaacg tcataatgct gatttaatac
     3061 tttatctgaa gcttcttttt tattgataaa acgcgtaaca aatgacatat cttctcgcat
     3121 tggttgacga atttgttgat taacatgttc ttgtaacgca tctgtcgctg ttaataatcg
     3181 ctgttgttga atttgtagtt ttttcttctt tttattaaaa aatccgccaa cattaaagtc
     3241 ttgagacata ctttccaaat aacttcgtaa catttctctc atattatgcg gcataatgta
     3301 tgcattttct aaaatatttt tacgcttatt ctttaaaaat gccattaatt catctggatt
     3361 atttaaaagt tgtgcctctt cactaattgc ttgatgttgt tgactattta aaaatgcttg
     3421 ttcaaactcc gcttcttcga tacctaaatc ttctagtact tcctgaattt cagactgaat
     3481 gtaatctagc tgagcttcgg taatgtattc aaccgttctt gatgtataat cctctattgt
     3541 ctctctatgt tgatctaatg aaattagata acttgataat acttcaagtt cattttcagg
     3601 gtgatcaaat ttagatacat aaaaggtgcg ttctaattta ataccccaat ctgcaattga
     3661 tttttcaact cgagatttaa acgtagagaa tgacaattca tcgtcttgat gcttgtcaat
     3721 ttgattaatg ataaacacaa caggtattcc aacatcattt atatgcttca taaacttaaa
     3781 gttaagttca gattgaacgt ggttatagtc aaccgtataa aatatcatat tacttgtata
     3841 catatattgt tctgttattg actgatgtga tgcaacattt gaatcaacac ctggtgtatc
     3901 ttgcaacgta aacccatttt caaatttagc tgattgaaaa ttaatttcta cagattcaac
     3961 gtcgacattt tggcgattca tttcccttac ttcatcataa ttagataatt tggcatacgt
     4021 ttgattcggc aaattagcaa taatatcgtg attgtctgaa actgacacaa tagcagtatt
     4081 acttgtcgtt ggtacaggtg aacttggtaa gatatcttgt tcaattaata aatttatcag
     4141 tgtcgattta cctgcagaaa aatgtccaac gaacgaacat gtatattgct gcaaatatac
     4201 tttcttaatt acttggttaa ttgtatgtaa aagtgcttca tttcgcgact tttcaacttc
     4261 tttttttaat ttatataaaa gatctaattg ttctttatta atcatgttac tttccccttt
     4321 ggttcgttca catagaaatg cttgcttgtt acaacgaatt catacaatat cgatgtccat
     4381 ttaaacatag atcgttaaat ggctcgcatt gtattatgtt taaattttat catagctgct
     4441 tgataaaaca tacgaataat caattttctt caaacattcc aacttaaaag ttcatttttc
     4501 taaaaatcac aaatcatcgt taaatttttc tagaaaataa atattgtcac agtttgttca
     4561 cacacatcac agaattgaca ccgtataata gccaattggg agggaaatat gaaaaataca
     4621 tttaatccat tacaaagatt acatttctat gcagcaatat ttattgctcc actgttaatc
     4681 actttaacca tttcgggcat tggttacttg ttctttccag aagttgaaaa taatatttat
     4741 aagaatgagt tttttggtga cagtgatgta aaaacgcatc aaacattaaa tgatgcagta
     4801 catcaagttg aacaacaata tgaaggattc tttgtaagta aagttagcat acttgatgaa
     4861 ccatataaca aacgaattac actgagtgat atggcaggaa atcaacgtta cgtctttcta
     4921 gatcataaca atcaatttgt tgcagatcaa aatgcgaaac atacgtattc taatgtgatg
     4981 cgaagtatac atagttcttt gtttactgaa aatactatta ttaattattt agtagagtta
     5041 accgcatgtt ggatgatatt catgatttta tctggtactt atttactcat taagaagcat
     5101 ttaatttcta acaaaagtaa ggcacttcgt tggcaaaagt ggcacgcaat gattggagtt
     5161 atcattgcaa ttccagtatt tgtattagtc ttaactggat tgccatggtc tggttttatg
     5221 ggcagtaaaa ttgccggtat gatggacaca aacggtgccc ttggtcaagg tgaattagcg
     5281 attaatccac ctaaatcaga tttgaacgaa ttaccttggg ctacacgtaa aaataaacag
     5341 ccagcttcat ccgaaaaagg ttcaagtggt catcatggta atgcagcaat gcctcaaacc
     5401 aaattagatt atcaaatatc tattgataag gtcgttgaac aggcgcaaaa agctggtatt
     5461 aaaaagccgt tttcaatcgt atatccaagt gataaaaatg gtacctttat tgtatctaat
     5521 actagtaatt caggtgttac tgggctagat gtatcaccat acaaggaaca aacactttat
     5581 ttcgatcaat atagcggtaa aaagctaggt acgattaaat atgatgacta cggtattatt
     5641 gctaaatggt ttacatgggg cattccgctt cacgaaggtc atttattcgg cattttaaat
     5701 aaaatcatta atttatttgt atgtatcgct ttattagtag ccattggcat ggggtttgtc
     5761 tcttggataa agcgtacaaa aaatactgca ataaaagtac cacatcgcgt aaaaaaacca
     5821 gcatctatat cactcataat atgtttaatt gtattaggat tattaatgcc attatttgga
     5881 ttatcactta tccttgtatt tataattgaa ttaatattat atattaaaga tcgtcgtgct
     5941 aaacaataat gcacttaaag ttttgaactg acgaaattta caaaatgatt ctcgtctctc
     6001 taattactta aaacgggttc tataataaat cgtactgatg gaaagttttt actttttatc
     6061 tgtccgattt tttgaatttg aagataaaaa aacatctaaa acgcataaga agataactct
     6121 acttcttcaa aattacgtta tagatgcttt tttattaatt catatgcatg tcgtgaattt
     6181 tcatcattaa accgtgtggg acgtcatcat gtttaacaat ctcttgttta taaaatgtct
     6241 tcccatcatc tttagaaact aattttacaa agtctccttt tttcggcttg aaatcattag
     6301 atggttctac tttgatatta tgtttgacta atccatcttt ttcactgaca actttttctg
     6361 catttgtatt actattcata taaccaaaat aagtcgtttt ttgactagaa aaaatcatta
     6421 aacatacaac ggcaatacca ataacaacca atgcaataga taaacctaga cccatttttt
     6481 tacccattta tagactcctt catttttcaa aaatacctat tatcatgaga acttaattat
     6541 tactaatttt agtcacgcat tagaaacact ttagttttca atagttttac gttctgtctt
     6601 tttaccccag tattgataat aagtacattc tatatatcca ttaaataact tacgtcgctt
     6661 tgttgcttta cgatctacta aatattcaaa ttctttatta cttgttaaaa tgtatgtaga
     6721 taaaaatgga tgttgtttca ttagtttacc aatataacgg tacatttctt caacttcttc
     6781 acgatcacca atacgttcac catatggagg atttccaatt aacgccaccg gttcttctgt
     6841 atcaattgtt aatgtattga catcttttac actaaattta ataatatcag acaatccaac
     6901 ttcttcagcg ttacgcttag caatctctac catttctgga tcgatatcag aagcatatac
     6961 ttcgatttct ttatcataat cagccatctt atccgcttca tcacggtaat catcataaat
     7021 atttgctggc atgatgttcc attgctctga tacgaactcg cgattaaaac caggtgcgat
     7081 attttgagca attaaacaag cttctatagc tattgttccc gaaccgcaaa atggatcaat
     7141 taaaggtgta tcacctttcc agtttgcaag acggattaaa cttgctgcca acgtttcttt
     7201 aattggtgct tcaccttgtg ctaatctata accacgtctg ttcaaaccag aacctgatgt
     7261 gtcgatagtc aataatacat tatcttttaa aatggcaact tcaacagggt atttggcacc
     7321 tgattcattt aaccaacctt tttcgttata tgcgcgacgt aatcgttcaa caatagcttt
     7381 cttagttatc gcctgacaat ctggcacact atgtagtgtt gatttaacgc ttctaccttg
     7441 aactgggaag ttaccctctt tatcaattat agattcccaa gggagcgctt tggtttgttc
     7501 gaataattcg tcaaacgttg ttgcgttaaa acgtccaaca acaattttga ttcggtctgc
     7561 tgtgcgcaac cataaatttg cctttacaat tgcacttgcg tctccttcaa aaaatatacg
     7621 accattttca acatttgttt catagcctaa ttcttgaatt tccctagcaa caacagcttc
     7681 taatcccatc ggacaaactg caagtaattg aaacatatat gattctcctt ttatacaggt
     7741 attttattct tagcttgtgt tttttataca tttccaacaa atttaatcgc tgatacatta
     7801 acgcatccgc ttactatttt aaaacaaggc agtgtcatta tatcaagaca aggcgttaat
     7861 tttaagtgtt cttttcatga aaaaagctct ccatcatcta ggagagctaa actagtagtg
     7921 atatttctat aagccatgtt ctgttccatc gtactcatca cgtgcactag tcacactggt
     7981 actcaggtga taaccatctg tctacaccac ttcatttcgc gaagtgtgtc tcgtttatac
     8041 gttgaattcc gttaaacaag tgctcctacc aaatttggat tgctcactcg aggggtttac
     8101 cgcgttccac cttttatatt tctataaaag ctacgtcact gtggcacttt caaattactc
     8161 tatccatatc gaaagactta ggatatttca ttgccgtcaa attaatgcct tgatttattg
     8221 tttcatcaag cacgaacact acaatcatct cagactgtgt gagcatggac tttcctctat
     8281 ataatatagc gattacccaa aatatcactt ttaaaattat aacatagtca ttattagtaa
     8341 gacagttaaa cttttgtatt tagtaattat ttaccaaata cagctttttc taagtttgaa
     8401 atacgtttta aaatatctac attatttgaa gatgtatttg ttgttgtatt attcgaagaa
     8461 aaacttttat tgtcctgagg tcttgatgtt gctacacgta gtcttaattc ttctaattct
     8521 tttttaagtt tatgattctc ttctgataat tttacaactt cattattcat atcggccatt
     8581 ttttgataat cagcaataat gtcatctaaa aatgcatcta cttcttctct tctatagcca
     8641 cgagccatcg ttttttcaaa atctttttca taaatatctt ttgctgataa tttcaatgaa
     8701 acatctgaca ttttttccac ctcattagaa actttgatct tcagaccact gtaagtcatt
     8761 gatgaatgct gttaattcat cgaacgtcac aatatcacaa gtatagtttg ttttatccat
     8821 aaaatcaact aacatctgct tgaagaactt aggactcgcc tcttgttcct catcataaat
     8881 gagcaatgtt tgatccgaat gttcaagcat aaattgatct gcttgtttaa attgaaaagg
     8941 accttgatac gacgtatgaa aaatactatc aacataatct gcatgcttaa ttatgttggc
     9001 atacttactt tgattatgtt cattccactt ttctgtatgt ccttggaacg gtgtaattac
     9061 agcaaacttt aacgaatcat atgttcgttg taattcaata acaacttctg cagtccataa
     9121 ttctataccc atttgccctt gtattaacac ccattctaat ccttcatcca acagttgttc
     9181 aattttatgt tttataaatt gttttaaata atgtacttca ggtgcgtcat ctttaaaaat
     9241 gtttaattcg aatgatttgt aacctgttac ataaactgtt ttaaccatca gtaccctctt
     9301 ttgttacgcc atctaatata ttttgtaaat catattgaac acttttaagc ttttctataa
     9361 ataactttcg acttgttcgc ttaaaatgac attcgacaga aagttgttca atgttagcaa
     9421 tcagtagctc aaatttcctt gaattcatat aaggtacttc tataataaat tcacgatgta
     9481 atttgatctc attgagtatg ctatcaatat gttcagtata tggctttaca gtttggtaaa
     9541 aatcatgatc ttgttgttgt gatttcacat tttcaaaatt ttgttgcatg ttgttaactt
     9601 cataaattag tgattcgact acatcattca tgaggacgcc tcctacattt tttaatttat
     9661 cacaatatac tgtattcgtc atgttttaac actctatata atttgattta actatttttt
     9721 caaatgtgtt atctgttaaa tcaagtaaat ctaaaaactt cctatataa
//
LOCUS       DAKTIP010000096         9616 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAKTIP010000096 DAKTIP010000000
VERSION     DAKTIP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.096"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0305_001683"
                     /gene_synonym="sfnaD"
     CDS             complement(122..2098)
                     /gene="sfaD"
                     /locus_tag="O0305_001683"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCY0274828.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPLIPSSLVATYPFNNESPIVTL
                     IKRYQSAASLSDFESSAKSWVETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2200..3393
                     /gene="sfaA"
                     /locus_tag="O0305_001684"
     CDS             2200..3393
                     /gene="sfaA"
                     /locus_tag="O0305_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCY0274829.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDASRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3380..5137
                     /gene="sfaB"
                     /locus_tag="O0305_001685"
     CDS             3380..5137
                     /gene="sfaB"
                     /locus_tag="O0305_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCY0274830.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5144..6214
                     /gene="sfaC"
                     /locus_tag="O0305_001686"
     CDS             5144..6214
                     /gene="sfaC"
                     /locus_tag="O0305_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCY0274831.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNHY"
     gene            complement(6315..6440)
                     /locus_tag="O0305_001687"
     CDS             complement(6315..6440)
                     /locus_tag="O0305_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274832.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6606..7589
                     /locus_tag="O0305_001688"
     CDS             6606..7589
                     /locus_tag="O0305_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0274833.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7601..8632
                     /locus_tag="O0305_001689"
     CDS             7601..8632
                     /locus_tag="O0305_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCY0274834.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8629..9597
                     /locus_tag="O0305_001690"
     CDS             8629..9597
                     /locus_tag="O0305_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCY0274835.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
BASE COUNT         3241 a         1347 c         1737 g         3291 t
ORIGIN      
        1 cctgtttttt gtgattttta ataatttgaa tatggaaaat gtattattct ctcatttgta
       61 tagattgtat ttaataagtt aatgtaatcc ttgagctcac gattaataaa attctataac
      121 cttaattatt ttctcgatac aaagggttat taactttaat ataagtataa tgatgtgcct
      181 catcttcaag acgcattgtt gtaacacact tataatctat aaatggcgcg aacatggtat
      241 ctttaatttc atttatgcga ttctcattta cttgattaga tttgtgtgtc gaaagtacaa
      301 gttgatcaaa aatgttatct agtacatcac gaacgatata ccacatatgt ctttctaagt
      361 ttgaatcatt tgatgcttta gagattgtaa gaattaattc gcctaaatgg ttttgaacgg
      421 tagaataaaa ggctttgtta aacacagacg tttttgaatc agtaagaatt cttgattttt
      481 catggaaatg agatgtactg tatcccattt catttagttg tgctttatca attcttaaac
      541 cttcaaagtc tctaatatac atcgtgtcca atagaccatc tttccgaaat gttgcaatag
      601 cattttgcaa gtgagcctct aatgcaatgc catatttagt aactagtggg attacgagac
      661 caagcaatgc tttactataa gtttcaaccc acgatttcgc cgatgattca aaatcagaca
      721 atgatgcagc tgattgataa cgtttaatca atgtcacgat aggtgattca ttgttaaatg
      781 ggtaggttgc aactaagctt gaaggaatca atggtgtgac ttcttgtgga atcatttggt
      841 atatattttt tctaaataat gtacctaatt gttcacttcg ttcagtttga tagtctgctt
      901 cgtcttgctc attataaaaa tgaataccag caacttcatc aataattgtt gatgcatagg
      961 acttaaatat gacatctttc tccaaaatat catttaaaat acgtgtcatt agtggaccat
     1021 tgtgcgtcgt ttgttctgat aatgtacgaa tctcacctgt aatatgaacg tttgtcgaca
     1081 atttgatgtg tggcgacata gctgggtatt taggaactaa tgttctgaaa gataaaccag
     1141 cataataatc caacgtatgt tttgcttcaa tgattaattc tttatctact tctgcttgat
     1201 aatcagaatg taatacgtca tctaattgcc atggatgaac aatcataatg tgataatcat
     1261 taaagttaaa ctttggcgta aattcatttt ctaattgttt aattaagtcc ggaaatagtt
     1321 gatgaacagt tgtatcataa tctttagaca gtgacatagt acggcttaat ttactgtgaa
     1381 tcagtactat tttcaactta ataggttgat tgaattctga agaatatagg aatgtttgta
     1441 atgcatttaa acctttacgt aatttagccc caggatgtag cggatgacct tcaataacgg
     1501 cttgctctga acgcaagtaa ctatcttcgc tattttcgat aatattaaat aaaggtgcag
     1561 aatcatgttg cattgacagt gcttgatagc taattgcaaa tgtcatatta gttgcactgt
     1621 ttattaaatc ttgctgaaat tgatcactag cagcattttt taaatctggt gcttcaatta
     1681 aaatacactc aagaatttca tttggatggt gtactcgtgt aatcgtattt gtaatgtcat
     1741 ctttaatgta gaaagggcct tcaacatcaa ttcgatcaaa ggcgtgttct ccagtgatag
     1801 gagcatataa tgtttgctta gcttgtggga actggatttc tagtatatga gtcgttgaga
     1861 tatctaacat aatcaaatca cgactcaata ttttcttact ttgagtgcgc gctttaacta
     1921 agttttcgcg atgcattgat gtgaccaatc tctgagtgac tttatctctt ccttgtaaaa
     1981 tcatctcttt aaaaatatta gcccaatcgc tattatgttg ttgtaaaaat aaatatgttt
     2041 cttgttcttc tttgttaaat tttaatgtct gttctttaaa aattaagttc aagttcataa
     2101 ttcacctcta tgaaatattt tacaaaagca agatagattt gtataatcca tattaatgat
     2161 aatgattctt attatcaaca gaatgcgggt gtaagtttta tgacaaaata tttttttagc
     2221 agttcttttc tactatttct aggtaattgg attggacaaa tagggctaaa ttggtttgta
     2281 cttaccactt atcataacgc agtttatctg gggattgtca atttttgcag acttgtacca
     2341 atattattac taagtgtgtg ggcaggggca attgccgata aatatgataa agggcgattg
     2401 ctgagaatta caatttcatc atcattttta gtaactgcaa ttttatgtgt gctcacgtat
     2461 agtttcactg caattccaat tagcgtcatt attatatatg cgacattaag agggatttta
     2521 agtgcggttg aaacaccttt aagacaagca atcttaccag atttatcaga taaaatatct
     2581 actacacaag ctgtatcatt tcattcattc atcattaata tttgtcgttc aatagggcct
     2641 gccattgctg gtgtcatatt agcagtctat catgcgccaa caacatttct tgcacaggca
     2701 atttgttatt ttatcgcagt tttattatgc ttaccattac attttaaagt aactaaaata
     2761 cctgaagatg catcaagata catgccgtta aaagttatta tagattactt caaattacat
     2821 atggaaggtc gacaaatatt tataacatca ttattgatta tggcgacagg tttttcatat
     2881 acgacacttt taccagtttt gacaaacaaa gtatttccgg ggaaatctga aatatttggt
     2941 atcgctatga cgatgtgtgc cattggtggt attattgcaa cgctagtttt acctaaagta
     3001 cttaaatata ttggtatggt aaatatgtat tatttaagtt catttttatt tggcattgct
     3061 ttgttaggtg tggtatttca caatattgtc atcatgttca tttgtattac attgattggg
     3121 ttatttagtc aatgggcacg tacgacaaat cgcgtttatt ttcaaaataa tgttaaagat
     3181 tatgaacgtg gtaaagtact gagtattatt atgatggata gaggtatgat tccattggga
     3241 agtctattaa tgagtatatg tgcagatgtg tttggcattg ttagaacttt ttcaataatg
     3301 ggaataagta ctatatgcat tacaatggta ttctatttta taaatagaaa gttgaagtta
     3361 aagttggagg aaagtaatca tggtatatct tgaatgggca aaggcagata gaaatattca
     3421 atatcgtgta attaacgcca ttattaaaga acgtatttac cccgagcaaa catttatttc
     3481 gcaaaaagga tctttaattg aaattcagta tcatatgcat gtgttgacta ttgaagttgt
     3541 tagaaaaagt gcattagaac gctatgagtt tacaggtgat attacttatt taaataaagg
     3601 tgaaacgtca ttaattataa ctttagaagg tttattagat gtgttgaatc atgactttga
     3661 tatccctatt tcagagcgac tacgcgaaga gttaatacac agtcgagata gtttagttga
     3721 aacatataag caaatgtctc acagacaaac gttaataagt caaagtttta aattttcaag
     3781 gttaccacaa gatattaact ttttttcatg gttacaacat gtaaaagata gtgataagac
     3841 agatgattta acttattctg agagtttggt accagagggg catccaacac accctttaac
     3901 caaaacgaaa ttgcccttaa ctatggaaga agtacgagca tatgcacctg agtttgaaaa
     3961 agaaatccct ttgcaaatta tgatgattga aaaagaccat gttgtgtgca cagctatgga
     4021 tggtaatgat caatttatta ttgatgaaat aattcccgaa tactacaatc agattcgtgt
     4081 gtttttaaag agtttaggtt tgaaaagtga agactataga gcgattttag tacatccttg
     4141 gcaatatgat catacgatag ggaaatattt tgaagcatgg attgctaaaa aaatattaat
     4201 tccaacgccg tttacaatac tttcaaaagc aactttatca tttaggacga tgtctttaat
     4261 tgataaacca taccatgtta agttgcccgt cgatgcacaa gcaacaagtg ccgttagaac
     4321 agtctcaact gtgactactg tagatggacc aaagttaagt tatgctttac aaaacatgtt
     4381 gaatcaatat ccaggattta aagttgctat ggaaccgttc ggtgaatatg caaatgttga
     4441 taaagatagg gcacgtcagc ttgcttgtat tataagacaa aagcctgaaa ttgatggaaa
     4501 aggtgcaaca gtagttagtg caagtctagt taataaaaat ccaatagatc aaaaagttat
     4561 cgtggatagt tacttagagt ggttaaatca aggaattact aaagaaagta ttacgacatt
     4621 tattgaacga tacgctcaag cattaatccc gcctttaatt gcttttattc aaaattatgg
     4681 aattgcttta gaagcacaca tgcaaaatac agtagtgaac ttggggccac attttgacat
     4741 tcaattttta gtgagagatt taggtggttc tagaattgat ttagaaacat tacaacatcg
     4801 tgtatcagat attaaaatta caaatgatag tttaatagct gattctatag atgcagtgat
     4861 tgcaaaattc caacatgctg ttattcaaaa tcaaatggca gaattaatcc atcattttaa
     4921 tcagtatgat tgtgttgaag aaaccgaatt atttaacata gtacagcaag tagtagcgca
     4981 tgccattaac ccaacactac cacatgcaaa tgagttaaaa gatattttgt ttggaccaac
     5041 aattactgtc aaagcgttgt taaatatgag aatggaaaat aaagtaaagc aatatttaaa
     5101 tattgagtta gataatccga taaaaaaaga ggtgtagtac tacatggcac acgttaacat
     5161 aaatatatcg aagattaaat ataacgccaa agtacttcaa acagtttttc aaagtaaaaa
     5221 tatgcaattc acaccagtaa ttaagtgcat agctggtgac cgtacaattg tagaaagctt
     5281 aaaagcgtta ggtatcaatc atgttgcaga atccagattg gataacataa ttagtattgc
     5341 agatcaggat ttaacatata cgttattgcg tacgcctgcc aaaaaagaga tttcagatat
     5401 gatagaaaaa gttgatatga gtatccaaac agaattatct accatacatc aaataaatga
     5461 agtagcagaa gttttaggaa aaaaacataa gattttatta atggtagatt ggaaagatgg
     5521 ccgtgaaggt gttctgactt atgatgtttt agattatatt aaagagatta ttcacttgaa
     5581 aaatatccat tttgtaggtt tagcattcaa ttttatgtgt tttaaatctg atgcgccaag
     5641 tgatgatgat atctttatga ttaatcgatt cgtctcagct gttgaaagag aaataggata
     5701 tcgattgaaa attatttcag gtggtaattc gagcatgtta ccacaacttt tatataatga
     5761 tttgggtaaa ataaatgaat tgcgaatagg tgaaacgttg tttagaggtg ttgatacaac
     5821 gacaaatcaa gcgatcgcta tgttatatca agatgcgatt acattggagg ctgagatttt
     5881 agaaattaaa cctcgagtta atacacaaac gcatgaatca ttcttacaag ctattgtaga
     5941 tattggttat ttagatacaa aagtcgataa tatttcacct atggatcagc atatcaatat
     6001 tttaggcgct tcgagtgatc atttaatgct tgatttaaat ggtcaaggac actatcaagt
     6061 tggagatcat atatcttttt cattaaatta tgaagcactt tctcattcga tgtatatgaa
     6121 aaatttacat aaggtatata ttgatgattc aaagattgat acgctacttc aaaattttga
     6181 tgtgaaatct cctgctatgg taaaccacta ttaatatatt tatcaataag tctaagttga
     6241 caagttatac tacatatcgt ttgcacggtt gtatcataat tgttcaactt agattttttg
     6301 tatttgttga tttatcaaat taagtgcaac agttcgtcac ataaaattgc aacagataat
     6361 atcagctgaa ttacagggat aacggtcatg ctaaatggtg tcaattgtat taatgcaaag
     6421 atgatatagc aatgatacat tatcagtatt ttgtctaagg aaatgtgcta attgtagtca
     6481 taattattag aggaaatata tagtcataca ttttagaaat acaaaaaaga ttgaacgcta
     6541 cttgacaatg ataattgtta tcaataaaat aataaatgaa gttatacata ttaaggagtg
     6601 gaacgatgag aggtctaaaa acttttagta tattgggatt aatagttgcc ttacttttag
     6661 ttgcagcttg tggtaatacg gataattcaa gtaaaaaaga atcatcaact aaagatacta
     6721 tttcggtaaa agatgaaaat ggtacagtaa aagtacctaa agatgcaaaa cgtatcgttg
     6781 tattagagta ctcatttgca gatgcattag cagcattaga cgttaaacca gttggtattg
     6841 ctgatgatgg taagaaaaaa cgtatcatta aaccagttag agaaaaaatt ggggattata
     6901 cttctgtagg tacacgtaaa cagccaaact tagaagaaat tagtaaatta aaaccggatt
     6961 taattatcgc tgatagcagt agacataaag gtattaataa agaattaaac aaaattgcac
     7021 caacattatc attaaagagt tttgatggag actacaaaca aaatattaat tcgttcaaaa
     7081 caattgctaa agctttaaat aaagaaaaag aaggcgaaaa acgtcttgct gagcatgata
     7141 aattaatcaa taagtataaa gatgaaatta aatttgatag aaatcaaaaa gtgcttccag
     7201 cagtagttgc taaagctggt ttattagcac atccaaacta ttcatatgtt ggacaatttt
     7261 taaacgaact aggatttaaa aatgcattaa gtgacgatgt aacaaaaggt ttaagtaaat
     7321 atttgaaagg accttactta caattagaca ctgaacattt agctgattta aatccagagc
     7381 gtatgatcat tatgacagat catgctaaaa aagattctgc tgaattcaag aagttacaag
     7441 aagatgcaac atggaaaaag ttgaatgcag ttaaaaataa tcgcgtggat attgttgacc
     7501 gtgatgtttg ggcaagatct cgtggcttaa tttcttctga agaaatggct aaagaacttg
     7561 ttgaattatc aaaaaaagaa caaaagtaag gtggaagtaa atggctataa aagaaataag
     7621 tagccaatct gccatagatc ataaaagaaa aagacgcaca acactcacgt atatagtgag
     7681 tttgtgcttt ctttttattt gtatatattt aaatatggcg attggttctt cgaaaattaa
     7741 ttttagcgat atcattcact atgttactgg tcatacagat acgaaagcaa cgtttttatt
     7801 gcataatgta cgtatgccaa ggatgattgc agggttattt attggcggtg cattagcggt
     7861 atctggtttg ttaatgcaag caatgacacg caatccgcta gcatcaccta aaatttttgg
     7921 tgtcagttct ggtgcatcgt ttttcattgt atttgtgacg attatcattc cagcattaga
     7981 atattatgca ttatatttag gtgtgatagg tgcatttata ggtggtttaa ctgtttatac
     8041 actttcaggt gctacgaaag gtatgacacc cattaaatta gcattggctg gtatggccat
     8101 ccatttgttt tttagtagta tgaccgaagg tattattatt ttgaatgaga attcaaacga
     8161 acaagtgatg ttttggcttg tcggttcatt atccagtatg aagtgggatg aaattttaac
     8221 cattctacca tggattattg gtgctttaat tgtaacgatt tttattggac gtcaactaac
     8281 gataatggaa ctaggcgatg acattgctaa gggattgggt caaaatataa ataaagttag
     8341 aattatcatt ggactattag tcattattct tacaggtatg tcggtgtcag tggcaggacc
     8401 aattggattt gttggcttaa tcgtaccgca tatagtcaaa cgctatgtga gtaagaatta
     8461 tttagtcatg attccattaa catttattat aggtgcagat ttgttactac tatcagatgt
     8521 attaagtcgt ttaattacat atccttatga atcaccagta gggattgtta catcattcgt
     8581 aggtgcactt tacttcttat ttataacgat taaaggggtg aaaagaatat gatgacaacg
     8641 aaaattatac gtcgttatag tttaatcaca atccttttaa ttgtgagtat attcataagt
     8701 ttatgtgtag ggtctgtcat gattcatccg attgatgcta ttaagggaat ctttacacaa
     8761 gatgatttta ttttaaatga gtatcgtata cctagaacat tacttggtat tattatcggt
     8821 agtagcttgg ctatttcagg tgcaattata caagctgtaa taagaaaccc gttagcctca
     8881 cctgatgtta taggtatttc taaaggtgcc agtttagctg ctgtgattat tattatgacc
     8941 tttccaacag caccattgtt tgtattacct ataggttcat tcttaggtgc ctttgcagta
     9001 agtttatttt tatcattttt aatttcaaaa tatgatgtta aaggttcaaa gcttgcctta
     9061 attggtttag caataggcgc aatatgtacg gcgattgtgc agttcttatt aatcagaaat
     9121 ccactggaag caaacaaagc gcttgtttgg ttgacaggta gtctatatgg acatgatatg
     9181 tcgaatattt atactatatt accttggttt ttaataacga taccaatcat cattttattg
     9241 agttatcaat tagatgtatt gaatctaggt gatgctgttg caaccgcact cggtttaaaa
     9301 gttaaaacta ttaaaatgct attgttaatt ttagcagtaa tattagcagg atccgcaatt
     9361 tcagttgtcg gtggtattag ctttttagga ttaattgcac ctcatattgc gagacaatta
     9421 ataggaaata aaacattaca cgttattatc atgtcggggt taattggtgc tatattgtta
     9481 acatttggag atggtttagc ccgtggcatt catccgccac tggatattcc tgttggggtt
     9541 atcattgcaa ttatcggtgc accatacttc ttaatattat tacgaagaat gaaataacta
     9601 acatatgatt agagtt
//
LOCUS       DAKTIP010000097         9609 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAKTIP010000097 DAKTIP010000000
VERSION     DAKTIP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.097"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..168)
                     /locus_tag="O0305_001691"
     CDS             complement(<1..168)
                     /locus_tag="O0305_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0274836.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLI"
     gene            complement(193..1557)
                     /gene="radA"
                     /locus_tag="O0305_001692"
     CDS             complement(193..1557)
                     /gene="radA"
                     /locus_tag="O0305_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCY0274837.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEVLSF
                     ALHS"
     gene            complement(2042..4498)
                     /locus_tag="O0305_001693"
     CDS             complement(2042..4498)
                     /locus_tag="O0305_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0274838.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(4512..5519)
                     /locus_tag="O0305_001694"
     CDS             complement(4512..5519)
                     /locus_tag="O0305_001694"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCY0274839.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(5509..6075)
                     /locus_tag="O0305_001695"
     CDS             complement(5509..6075)
                     /locus_tag="O0305_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCY0274840.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(6094..6555)
                     /locus_tag="O0305_001696"
     CDS             complement(6094..6555)
                     /locus_tag="O0305_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCY0274841.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            6713..7927
                     /locus_tag="O0305_001697"
     CDS             6713..7927
                     /locus_tag="O0305_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCY0274842.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(8135..8695)
                     /gene="pdxT"
                     /locus_tag="O0305_001698"
     CDS             complement(8135..8695)
                     /gene="pdxT"
                     /locus_tag="O0305_001698"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCY0274843.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(8699..9586)
                     /gene="pdxS"
                     /locus_tag="O0305_001699"
     CDS             complement(8699..9586)
                     /gene="pdxS"
                     /locus_tag="O0305_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCY0274844.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
BASE COUNT         2818 a         1896 c         1339 g         3556 t
ORIGIN      
        1 aattaagaac ataaaaatac taccgataat gccatctaca tagtgatttt ttaaaaagct
       61 ggagttttgt aatccaagat catttgcaat ttcaggaata ataattattc ctaatgcgct
      121 cccaataatt aagtaaataa taataaccat tagtttaacg atattcacac aatgtcctcc
      181 tttcttgatg ttttatgaat gaagagcaaa tgacaatact tcatgtacag tagttacacc
      241 tattacttgt ataccttcag gatatgtcca tccgcctata ttatttttag gaataattac
      301 acgtttgaaa cctagttttg cagcctcttg cacgcgttgt tctatccgag atacacgacg
      361 tacctcaccc gttaaaccaa cttctccaat atagcaatct aatccgtcga cagctttatc
      421 tttaaagcta gatgcagttg ctacaattac acttaaatca actgctggct ccgttaactt
      481 tacaccgcca gctactttga tataagcatc ttgttgttgt aatagataat tttctttctt
      541 ttccaaaaca gccatcaaca aacttaatcg attatgatca atccctgttg ccattcgtct
      601 cggattgtta aaagtcgttg gagttaccag cgcttgaact tctattaaaa gtggtctggt
      661 tccctccatg gttgcaacaa ttgttgaacc tggaacattt gttgaacgtt cttctaaaaa
      721 catttcagat ggattattta caccttttaa tccactttgc ttcatttcga agattcccat
      781 ttcattcgtt gaaccaaaac ggtttttaac agctcgcaaa attcgatatg cgtggtgttc
      841 atcgccttca aaataaagca cagtatcaac catgtgttct agcaatcttg ggccagcaat
      901 ttgaccttct ttcgttacat gacccactat aaaagttgca atgttcattt gtttagcaat
      961 attcattaaa ctttgtgtac tttcacgaac ttgtgaaaca gaacctggcg cagagctgat
     1021 ttcaggatga tatattgttt gaatcgaatc cactactaat aaatcaggtt gttcttcttt
     1081 tactgtttga taaataactt caagatctgt ttcagctaat acttgcaatt cacttgaatc
     1141 ttcatctaat cgctctgcac gtaatttagt ctgactaagc gattcttctc cagtaatata
     1201 tagtactttt ttcttttgag ataacgatgc acaaatttgt aaaagtaacg ttgacttacc
     1261 aatacctgga tccccaccaa taagtactaa cgatccgctc acaatacctc cacctaatac
     1321 acggttgaat tctgctgaat ctgttaacac tctcggcgtt gtttcatgtt taatactatt
     1381 taatttttgt actttacctg ctaattcctt ggttttaact ccatgtttag gattggctgc
     1441 tttttcaaca atttcctcca tttgattcca agcgccacaa ttaggacatt tccccatcca
     1501 tttaggagat tgataaccac aagccataca ttcaaaaatc acttttttct tggccaaaat
     1561 tgcacctcca ctttcctttg aacaaatcta ttttaaacgc acattcccat tatgacaaat
     1621 taaatgtgag ttacatttgt ttgtatttta acatgactac taacgcaaac tgttttacca
     1681 gatttatttt ttatgaatat tataaaataa acttacatca tgttgatagt cggtatcttt
     1741 caaagataaa atcttaataa tttcttagta aactcttttc tctagattta tcacaatatt
     1801 atatagacct attttatttt gacgtaagtt gctagtatct tcaaacaaaa aacctttata
     1861 aaaattcata cctttatgct atcgctgtag gctcattaac ttgttacata taattcttaa
     1921 ctatcctttg atgattgttt tattagattg tttcgttgat ggatactttc acgaatttct
     1981 atagttcaat gctactaaaa aaacagccca aaactttaat ttgttttgga ctgttttata
     2041 attatgcttg cgatggtgtt ttagtttctg aagtttgttc agcaatgtca tatttaaact
     2101 ctttaccatc atgatctact gtaactttct taccttcaat ttgattacca tctaatatta
     2161 attcacttaa attatcttcg atagtttttt gtatcgctct aattaatggt cttgcaccat
     2221 attctggatc atatccttct tctgcgattt tgtctttcgc tttatcagtt acaataatat
     2281 ttatgttttg ttcagataat cgatttgtta atttattaac catcattgtt acaatttctt
     2341 ttaattcttc ttttgttagt ttatggaata caatgatatc atctacacgg tttaaaaatt
     2401 ctggacggaa tgaatttttt aattctttta acatcgtttt tcgaattgtt tcataatctt
     2461 gtccatcact tgaaccaccg aatccagcaa atcgttgatc ttgtaattct tgtgccccaa
     2521 cgtttgatgt cattatgata attgtatttc tgaaatcaac tgtacgtcct tttgtatctg
     2581 tcaaatgtcc atcatctaaa acttgtaata gaatattaaa tacatctgga tgagcttttt
     2641 caatttcatc aaataaaatt acagaatatg gtttacgtct aactttttca gttaattgtc
     2701 caccatcatc atgaccaaca tatcctggag gagcaccaac taatcggctc actgcgtgtt
     2761 tttccataaa ttcactcatg tctacacgga tcatcgcatc atcatcgcca aacattgatt
     2821 cagctaaagc tctagctaat tcagttttac caacaccagt tggtccaagg aagataaagc
     2881 taccaattgg tcgtttagga tcttttaacc ctgcacgggc acgtctaacc gctttactga
     2941 ttgaattaac agcatctttt tgcccaataa ctctctcatg taatgtatct tctagactaa
     3001 gaagtttttc agattctgtt tcattgattt tagttaatgg gatacctgtc catcctgcaa
     3061 taacttcagc aatatcttct tctgacaatg aagttgacat gccattttgt gcattcttcc
     3121 attcattttt agcttcttca tattgctttt caagttttgt ttgtttatca cgcaggttag
     3181 cagcattttc aaactcttga gcatgtactg cggcatcttt ttcattttta actttttcaa
     3241 tttcttgttc aatttctttt aaattattag gtgtcgtatg actcttaagt cttactttag
     3301 aacttgcttc atcaattaaa tcaattgctt tatctggtaa gaaacgatct gaaacgtatc
     3361 tgttacttaa tttaacagct gcttcaatag cttcgtctga aatattaata cgatggtgtg
     3421 cttcgtaacg atctcttaat ccttttaaaa tagcaactgt atctactact gaaggttcat
     3481 caacttgtac aggttggaaa cgacgttcta aagccgcgtc tttttcaata tttttgcgat
     3541 attcatctaa tgtagtagca ccaatacatt gtaattcacc acgtgctaat gccggcttca
     3601 aaatattcga agcatcgata gcaccttcag caccaccagc accaactaaa gtatgcaact
     3661 catcaataaa taggatgaca ttacctgctt gttggatttc ttccataacc tttttcagac
     3721 gctcttcaaa ttcaccacga tatttagtac ctgcaactac tgttcccata tctaaagaca
     3781 taacacgctt atcttttaat gtctctggta cctcattatt cactatggct tgcgctaaac
     3841 cttcagcaat agcagtttta ccaacacctg gctctccaat aagcacagga ttgtttttcg
     3901 tacgtctact taatacttca attacacgtg taatttcttt atcacgtcct ataacaggat
     3961 ctaatgtacc gtctttggca atgactgtta agtcacgagc taaactatct aaagttggag
     4021 tattatttga cttactagct tgtgcatttt tattactcat ttcagggttt cctaaagctt
     4081 tcacaacttg tgcacgtgct ttagtaatat ttaaatctag atttgcaaaa actcttgctg
     4141 caacaccttc attttcacga atcaagccta ataaaatatg ttccgttcca acaaaattgt
     4201 gatgtaattt tctagcttca tccatcgata attcaatgac ttttttagct ctaggtgtat
     4261 aatgcaatgt accaacatga tcttgaccat gtccgattaa tttttcaact tcttcaatta
     4321 ctttatcttc agtgatatta aaactttcta atacttttgc agcaattcct tcaggttctt
     4381 tcattaaccc caataatagg tgttctgttc ctatatttga atgatttaaa cgaattgctt
     4441 cttcttgggc atgtgctaat acgcgctgtg cacgctcagt taatctacca aataacataa
     4501 ataatgacct cctactttat atgttctctt agtatatctg ctcgtttttc ttttacagat
     4561 ttgtcatctt cttcatctaa taaaaatggt gactgtatag ctaccatcaa ttcattaaat
     4621 ttaaagtttt gtaattcaat gtaatttaaa tctataccaa gtttaacttc gcttaatcta
     4681 taagaagcct cttccatagt tatcattcta cagttttgta aaatacctag cgagcgaaaa
     4741 acacggtctt gtgtttctaa ttgattataa gtgtctaact tttgtcgtat ttgtttttct
     4801 tcatgaatga tttgattaac aacttctgtt aatgtttcta tgatttctaa ctcagattta
     4861 ccaagtgtaa gttggttgga tacttgataa gtatgtccat aaacttgcga accttcaccg
     4921 taaatacctc tgattgtata tccaaaacga ttaatggttt gagcaatccg tgtcattctt
     4981 ttcataatag atagacctgg caaatgtagc atcacgcttg ctctcatacc agtacctata
     5041 ttggtaggac atgtagttaa ataaccaagt tgttcatcat aacttatatc aaggcttcga
     5101 tctaattcat catcaattga tgaagcttga ttatataaag cctgtaatgt cgtgtcagtt
     5161 cccatagctt gaatacgaat atggtcctct tcatttatca tgacacttaa agattcatcg
     5221 tcattcacta atactgcggc tgctggttgt tttattagtt caggactaat caaatgcttt
     5281 gcaaccattt tcattttact ttgttgatcc atttgatcaa gacgcattaa ttcaaagttt
     5341 ggcaaggcat cttgtacctc atttataact ctaaatccat cattttcagt agcatacatt
     5401 agtggatgca catgattttc taaattacgc gctaaccgaa ttctagaaga cataacaatt
     5461 ggtgtttctt cattactttt catccattgg ctgatattat catgaatatt atgcgtcatc
     5521 atgttgcacc tcactctcag cttttagtgc tttaatttca tctctaacaa tggctgcttc
     5581 ctcaaaatct tggatttcaa taagtttttt caaatattca ttcttttctt cgatttttcg
     5641 ctttaaagct atctttttat gtgaagaatg tggtgtcttt ccaacgtgct caaattgtcc
     5701 accttgaact ctgcggacga tatcaatgat gtcatcttta aatgttgcat aacaattagc
     5761 acacccaaat ttaccaacat gtgcaatatc tttcaaagtc atatgacatg atgggcaacg
     5821 tttttcttct ttaaacgcta cttcttgaaa attaattcca tgtttcgtag ctaaatgttg
     5881 taaaatttgc ttaacaacaa atgcttcttc gaaattatct tgatgttctt gatattcagg
     5941 ttgttcatta gcttgattcc acggatggtg tccctcagca caagtttgac acaccatttt
     6001 ttcttctgtt ttatttttac ttgtaacttt aacttttaat tccgcttcat taagttgaca
     6061 attttcacaa agcacttcaa cacctcattt catttagtaa taatttataa ctggtaacaa
     6121 acgttttaaa atatttgctc taataatatc tctagaaacc atatccattg atagcgtttc
     6181 tctatcaata actgcttgaa tcattttagc ttcacgttca tttattaaca ttttatctaa
     6241 aagcccatca ataatataat aagcttgttg ttgagaaata gaaggtccaa tcagctgaag
     6301 caaatgatta atataacctg ttgcatcttt attttcaatt ttagtgattc ggatgtaacc
     6361 accaccacca cgtttacttt cgatttcata accatgttca ttagtgaatc gtgttttgat
     6421 tacataattt aattgtgatg gtacgcaatc aaaacgctgt gcgatattcg ctctctgaat
     6481 ttcaacgaca tcttcattcg actcttcaaa taaacgtttg atgtattgtt ctatgatgtc
     6541 agacatattg tgcatgtata tcaccccttt ttgaccttct ttgactatat tatatgacct
     6601 attttgacct ttttcaatta atttgttttt atattttcaa aaatttatga tgttagccct
     6661 tccattttat tgtatgatag attgtagaaa aaactaaagg aatgagaaat atatgcatat
     6721 tctaattggg attatcggaa ttatcttctt tttagcactt gcgtttctat ttagctcaga
     6781 caggaaaaat attcgctgga aatatgtagg aattttactt gcaattcagc ttatctttgc
     6841 atttatcttg cttaaaacta caatgggaat caaggttatt ggtggtattt caaacggttt
     6901 taactactta ttattaaaag cagcagatgg tgttaacttt gtattcggtg gtattcaata
     6961 tatcgatcct aagcaaccac cattcttctt cagtgtatta ttacctatcg tatttatttc
     7021 agcgattatc ggtatccttc aatacacaaa gattttacct ttaatcatta atgttttagg
     7081 attcttaatt tctaaaatta atggtatggg acgtttagaa tcatacaatg cagttgctgc
     7141 agcaattttg ggacaatctg aagtatttat ttcaattaaa aaacaattac catacatacc
     7201 taagcaacgt ttatacacat taacagcttc agcaatgtcg actgtttctg cttcaattat
     7261 cggtgcatac tttaccttaa ttgaacctaa atatgttgta acggctgttg tcttaaactt
     7321 atttggtggt ttcatcatcg cttcaattat taacccttat aaagttaatg aagaagatga
     7381 caaattatta gttgaagaaa ctgaagaaag acaacaatca ttcttcgaaa tgctaggaga
     7441 atatatttta gatggattta aagtagctgt cattgtaggc gcaatgttaa tcggatacat
     7501 tgccattatc gctctattaa atggaattgt aagcaatatc tttagtacag tttcaggtgg
     7561 cgctatttct tgggatttcc aaacattaat tggttttgtc tttgcaccat ttgcattctt
     7621 agtcggtgtg ccatggcaag atgcagttca agccggttca gttatggcga caaaactatt
     7681 atctaacgaa tttgtagcaa tgcaagcact tggtaaactt agtgatttat ctgaacacgc
     7741 aaaaggtgtt acatctgtat tcttagtatc atttgctaac tttagctcta tcggtattat
     7801 ttcaggtgcg attaaatcac ttaatgacaa aaaaggagat acagttgccc gctttggttt
     7861 gaaattatta ttcggtgcaa cactagtatc tttcatttca gctgcaatcg ctggattctt
     7921 tatttaacaa atacatcaac atcgaattaa ccccttcaaa attttgacta ttctcagtct
     7981 ctttgaaggg tttttttctt atgtatagct ttatttttta tcatttttaa ttttgacata
     8041 aaaaataact cctccaaagc taagtttgaa ggagatattt tcttctcaac aaatctataa
     8101 atatcaataa ttcgttgatt tagcatacat taagctatgc tttttttaca atatgattaa
     8161 taaagtaatc agttactcta tagtcatctg ttaattcagg atggaatgat acgcctaaat
     8221 atttaccttg ctgaacagct acaattttct cattaacctt acataggata tctacgcctt
     8281 gacctacttt ttcaatatgt ggggctctta taaagacacc ttcaatatct gtagcgatac
     8341 ctttaatatc taattctgtt tcaaagctgt caacttgtct accgaatgag tttcgttgta
     8401 cagtaatatt caacttgtta aggtatcctt cttcaccaac tatatcttgc gctagaacta
     8461 ttaatcctgc gcatgtacca aacataggta aagttgaatt ttgtaaagcc tctttaaatc
     8521 catataaatt cattaatcga cgtaacgttg tagactcgcc accaggtaat attaagccct
     8581 cgatttcttc taattgttca acttttttaa ctgcaatacc ttcatgacca cttaattcaa
     8641 tatgtctaat atgttcacgt actgcacctt gtaatgctaa tacacctatt ttcatatctt
     8701 accaaccacg ctcttgcata cgttcttcta atgataattg attgatatct aaacctttca
     8761 tagcagtgcc aagttcactt gctaatcttc caattagttc atagtcttgg taatgtgttg
     8821 ttgcttgaac aattgcttta gcaaattttt ctggatcttc tgatttaaaa atacctgatc
     8881 caacgaatac accgtcagca cctaattcca tcattaaagc agcatcttga ggagtcgcaa
     8941 cgccaccagc tgcaaagtta actaccggta aacgaccatt gtctttaatt tgttttaaaa
     9001 tttcataagg cgcaccgata tctttcgcaa aagtcataat ctcatcatca ttcattacag
     9061 tcaatcgact aacttctgaa ttaacttgtc tcatatgtct tacagcttca acaatattac
     9121 ctgtacctgg ttcaccttta gtacgtaaca tagcagcacc ttcaccaatt ctacgcgcag
     9181 cttcacctaa attacgacat ccacatacaa atggtactgt aaattgatct tttcttaagt
     9241 gatattcctc atctgctggt gttaacactt ctgattcatc aatatagtca acacccatcg
     9301 cctctaatac tcttgcttca gtgatatgac caatacgtgc tttagccatg actggaatag
     9361 aaacagcatt cattacttct tctacaattt tagggtttgc catacgtgca acaccaccag
     9421 cagctctaat atcagaaggt actcgttcta atgccataac tgctaccgcg ccagcttctt
     9481 ctgcaattct tgcttgctca gcattaacga catccataat aacgccgcct ttttgcattt
     9541 cagccatacc tcttttgact ctgtctgatc caataatttt actcattata cataaaaccc
     9601 cttttcaag
//
LOCUS       DAKTIP010000098         9561 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAKTIP010000098 DAKTIP010000000
VERSION     DAKTIP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.098"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(289..1035)
                     /locus_tag="O0305_001700"
     CDS             complement(289..1035)
                     /locus_tag="O0305_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCY0274845.1"
                     /translation="MPIEYFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVESHS"
     gene            complement(1040..1513)
                     /gene="ytkD"
                     /locus_tag="O0305_001701"
     CDS             complement(1040..1513)
                     /gene="ytkD"
                     /locus_tag="O0305_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCY0274846.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEIADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIYYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            1579..1836
                     /gene="yidD"
                     /locus_tag="O0305_001702"
     CDS             1579..1836
                     /gene="yidD"
                     /locus_tag="O0305_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCY0274847.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(1833..2834)
                     /gene="menC"
                     /locus_tag="O0305_001703"
     CDS             complement(1833..2834)
                     /gene="menC"
                     /locus_tag="O0305_001703"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCY0274848.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDEVADGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGAKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(2839..4317)
                     /gene="menE"
                     /locus_tag="O0305_001704"
     CDS             complement(2839..4317)
                     /gene="menE"
                     /locus_tag="O0305_001704"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCY0274849.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNSLLDNTMGIQYETSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNVYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(4476..4931)
                     /locus_tag="O0305_001705"
     CDS             complement(4476..4931)
                     /locus_tag="O0305_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCY0274850.1"
                     /translation="MKICLMLYLLIFLSSCSTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            5234..5884
                     /locus_tag="O0305_001706"
     CDS             5234..5884
                     /locus_tag="O0305_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCY0274851.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTNNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNHHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            5965..6960
                     /locus_tag="O0305_001707"
     CDS             5965..6960
                     /locus_tag="O0305_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCY0274852.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMPHSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            7036..7662
                     /locus_tag="O0305_001708"
                     /pseudo
     CDS             7036..7662
                     /locus_tag="O0305_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7703..8047
                     /locus_tag="O0305_001709"
     CDS             7703..8047
                     /locus_tag="O0305_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCY0274853.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIENAEHTLFTPYM
                     LETLSSLGVKDSIVDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRIIQEK"
     gene            8145..8696
                     /locus_tag="O0305_001710"
     CDS             8145..8696
                     /locus_tag="O0305_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274854.1"
                     /translation="MEKNEYIAKYNEYSQLLDATYSQAVAYLLSKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTRASEGLYCHHISEDKFQNLSDLRFISKFKYSYDVQKKENL
                     VYCDLIEHLILHAIITKESHGQFGVAGLCQMIKPTVIDWYIGEYNPKPAWMQATKARA
                     YLPGILVEKLLIKIDDMLKGIEI"
     gene            complement(8914..9555)
                     /locus_tag="O0305_001711"
     CDS             complement(8914..9555)
                     /locus_tag="O0305_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0274855.1"
                     /translation="MFTPIVINLLNDVGSIFLVRVGNQLKVIEKVHYEDILESKIVRL
                     FQAFIKDKMDQLFITSEHFAIDIDKLLFQLNLNVRYIKMEGNTSGYLSGRTIYINNNH
                     SINNTRFSIARELGRYLFKNTDLMRDSNDNSLKNLHEMIYESDVNQFSVDLLMPKKQI
                     EALVYNFYEVNNINLSSGLSEKERNKLLNLISTKLEVSKVAAGLRLYNLGIHI"
BASE COUNT         3301 a         1729 c         1471 g         3060 t
ORIGIN      
        1 tagaaccttt tctgaaccat acgcatagcg tatggttttc tttttacaat taaagagcca
       61 accgttgtta tagtctaaca atggttggct cctcttattt tatgtgctaa aaatttatag
      121 gcaattttat tacaacaatg tacatttaag gtgaccttca tgccaaaatc gcatcactca
      181 tttaatggaa gcagcacgtc ttcatataaa gtaccgatcc ctaattcaac gcatgtagta
      241 ccacatcttc aaagcttgat agttcccatg cgcacaccac gtttcatact agctatgcga
      301 ctcaacttgg ttcataaact ctttaatata agtcaatgtt tcaaccatcg ctggtggtct
      361 tggcacatgt ccttctgcca tttgataaaa tgtttcatgc gtggcacctt ttaactctag
      421 ttggtccgct aaataatacg catgatgaat accaacttgc tggtctttcc ctccatgtac
      481 aattaatatt ggcggactgt tttcattaat gtttggaatc gcttggcgtg cctcatatgc
      541 cgctcgatct tttttcggat gaccaatcat tcttcgtagc atgcctctta aatcgacacg
      601 ttcttcatac attaaatcaa tatctgagac accaccccag attgtataac ttgttactgg
      661 taagtcttga aatgtcaaca atccttgtaa accacctcgc gaaaaaccaa ccatgtggat
      721 aaatgcatgt ggatatttat catgtagcaa ccttaataat tgcgtcacat catttaaatc
      781 gccacggtaa aattcgtctt tgccttcact cccattgtta cctcggtagt atggcccaat
      841 cactaaagtt tgactatctg aaaattgcat taatctacct gcgcgcacac gtcctacttg
      901 acctttgcca cctcgcaaat aaactacaat gcgatttact tcatgatgtg gtgtcatcat
      961 taaagctttt acttgtaagt catctgacaa atatgtaatt tcttcgaatt gatgcgtaaa
     1021 atattcaatt ggcattcgtt tacgtttgat aaaacccaag tgattgcacc ctctctacgc
     1081 attttaaaat ggtactatct tgcagtaaga aactccgttg tgcgagttca atatcattga
     1141 tacagttaaa caacactggc cctgctgttt ctaaataatc gttcttgctt accaatgatt
     1201 caacttcgat aaaatataca tcttttacaa aatcagtttg atcatgtgtt tcaatggtat
     1261 attgtgctat gtaataaata tttttaactt tggcgcctgt ttcttcatat aattcacgtg
     1321 taactgcttc agcactactt tccccgcgtt cccttttacc accaggaaat tcaatccccc
     1381 gtaaattatg tttggtaaaa agcaattgat ttttaaacgt tggaatagct agcacatgat
     1441 tgccatctgc tatctcatta tcctttttaa atgtcaaatt aacttgacga ttatctttat
     1501 ccctaaactt cacgcgcatc acatccctac attgtatgtt aatataatag ttaattacta
     1561 tcgttggagg cattaattat gaaaaagata ttcttggcga tgattcattt ttatcaacgt
     1621 ttcatttcgc cactcactcc accaacttgt cgtttttatc caacatgttc agagtacact
     1681 agagaagcga ttcaatacca cggtgctttc aaaggccttt atttaggtat ccgtcgtatt
     1741 ttaaaatgtc atccgcttca taaaggcggc tttgaccctg ttccgttaaa aaaagacaag
     1801 tcagcaagca agcattcaca taaacataac cattaatatg gttgtaattg agttatatcc
     1861 actaaagggg ggcgaaattc gagtcgcccc tcttttaata tgcctgaatg cgccaccaca
     1921 tcttgttcaa aataataacc tgctggtgta acatctcctg gataatcacc tttacgagca
     1981 agcatcgctg taaaatagcg gcttaaacca tattcgtaca tgccgccaat aaccactttt
     2041 gcaccatgac ttttcaaagt atcaattgcc gtttgcactt tatcaatgcc acctagacga
     2101 aatggtttta atacaacaac tttcacattg tataattcta tcaaattaat tatgtccaac
     2161 aacgatgttg ccttttcatc aagggctatt ggaggtattg ttccatccgc tacttcatca
     2221 agcatggaga tatctttaaa tggctcttcg atataaagaa cctgttcacg cgctaataac
     2281 tgtaactgtg tgaaatcttg acgatccaag gactcatttg catctataac caattgaaag
     2341 tgaaagtcta attcccgtaa cactctaatt tgatgcatga tttgaggcgt ccattttaat
     2401 ttaattctgg tcggctttgt tgcttttaat gactctagtt gtttatttga taagccgctc
     2461 gctgtcgctc catatgctac tgaaaatgaa ggcagtacat gaaacatttg atacaatgcc
     2521 atgacaatag ttgcccttgc agcaggcgta ttttccaatg aatctactaa ttttagtgct
     2581 gcttcatacg tttcaaatga tttatttcta ttatcttcga accattgctc aattacatgt
     2641 ttcactgagg caattgtttc atgatcatac caatctgttt gaaaagcgtt acattccccg
     2701 aaatatgcat ttcctttgtc atcaatcaat tcgataaaca aacaatcacg atgcgttaaa
     2761 gtgactttcg gtgttacaat ttgtgactta aatggctcac tatatttata aaaatgcaaa
     2821 gctgtcaact tcatcaaatc atcctctata caacttattt ctttgtaatt tacctgttga
     2881 tgtataaggt aaagtatcaa ccttttcaaa gtgtttcggt actttatatt tcgctaaatg
     2941 ttgtgataaa tatgcaatca attgtgcctt tgaaatgtca ctttcactga caaaatataa
     3001 tttaggcact tggccccaag tatcatcagg atgccctaca catactgcgt cactgatacc
     3061 tggaaattgc ttcgctaccg tttcaatttg atatggataa atattttcac cgccactaat
     3121 aattaaatct ttacgtcggt cataaatcat gacataacct tcatgatcta tttcagcaat
     3181 gtcacccgta ttaaaataac cattttcaaa cgtacccgtt aaatctgttg gatacaaata
     3241 tacattcatc acattggcgc ctttaatcat taattctcca tgaccttctt tattaggatt
     3301 tttaattttt acgtcaacat tggcacttgg catccctaca gtgtcaggac gtgcatgcaa
     3361 catttccggt gttgctgtta aaaattgcga acatgtctca gtcataccaa atgaattata
     3421 aattggcagg ttatattgta atgccgtctc tatcatagtg gcagataatt tagcaccgcc
     3481 gagtaatatt ttttgtaaat tataaggttc atgtaaacct tgttgcataa gccaatttaa
     3541 agtttgtggc acaagcgaaa tgtgcgtgat tcgttcattt ttaatcatcg ttaaaatttg
     3601 ttcggcattg aatttatcaa caatgcgcac agtaaaacct tcaataacag ctcttaaaag
     3661 tacactgaga cccgaaatat gataaatcgg caagacagat agccaattag tgtcacgatc
     3721 aaatcccaag ctctctttac atccgattgc actggcataa tgattacgaa acgtttgtgg
     3781 caccgctttt tgagggcccg ttgtccctga tgtaaacata atcgatgcaa tgtcatctaa
     3841 attaaatgat gtatttaata tgttggacgg cgactctttc ggcaccacag tttcattcga
     3901 tgtttcatat tggataccca ttgtgttgtc caacaaactg ttcgttgtaa tatcccttcc
     3961 agcgaattca atatcatcca gcgatacaat ttgaaaccct cgtaattcca gtggcaaggt
     4021 acaaaaaatc aattgtacat cgattgactt catctgattc gtcatctcat taggtgtcaa
     4081 ccttgtatta atcatcgcaa tttcaatatt tgccaaccaa catgcatgta ttaaaatgat
     4141 cgattgaatc gaattatcta tgtatagccc aacacgagat tgttgataag ccttgagtct
     4201 tttagccaat agactcgctt cacagtataa attttgataa gtataagatt cttgaccgtc
     4261 tgttatcgca atatgatgtc cattttgttg tgcttgttta tataaccaaa agtccatgcg
     4321 ttattcctcc aaaatcattt acattataat tataacgatt ttatgacatt ctagcagtgg
     4381 ttatgtttaa aaatataaaa aagtagacga attgatgcat tgatatgatt gttataatgc
     4441 tcaatacata tcgttatatc attcgtctac tattatcagt tatttttatt taattttagt
     4501 gtcattctgt cattttgatg tggtgattta cccattgttg ccacatcatc tgcaatgtca
     4561 attggtatac ggttcatgtc ttgtaatgca cttaaatgga atacttcatc atctaaattt
     4621 tcaatgagat atacataata tgttaccttg tcctttttat attttaacgt tttccaaaag
     4681 tccggcttgc aattcaatac attatccgga atatattcaa taaataagta acgtttgctg
     4741 cctactttgt ctatgaaata ttttgcagtg cctttttcta tacctcttat atgtgcatag
     4801 tctgctgaaa agtaaatact acctattgtt tcattatgtt gttgtatttc aaatcgttgg
     4861 cctactattt tattatttgt gctacaacta cttaaaaaaa tcagtagata tagcattaaa
     4921 catattttca tccccttgaa ttttaaaaac tttttcaaaa gcaacacctc taaaaataaa
     4981 tacaatatat tatagcaaat agaaattatt attttgttaa attatgttgt catgttcgtg
     5041 tcattttgag tcgtaataca actttacaat tattaaatta acacttctac aatccaaaaa
     5101 tatttttaaa gttttgaaaa aaattaaagt atttccacta ctaatttggt aaaatatgat
     5161 tatgtgatat agtgaaagcg ttttacactt aataactccc tcttaaatgc atccaggttt
     5221 tatgtagtaa atcatgaaaa taacatataa atatagagga gatttacctt tgaatacaga
     5281 gaacaacaag aatcaaaacc aatctgttaa aaattctgaa agacgcggca tgttaaaagg
     5341 atgcggcggt tgccttattt cttttatttt attaataatc ttattatcag cctgttcaat
     5401 gatgtttagt aataatgaca attccactaa taatcaatca tcaaaaacgc aattaactca
     5461 aaaagatgaa aataaaaatg aagataagcc tgaggaaaaa tcagaaacag caacagatga
     5521 ggatttacaa tcaaccgaag aagtacctgc aaatgaaaat actgaaaata atcaacatga
     5581 aattgatgaa ataacaacaa aagatcaatc agacgatgat attaacacac caaacgttgc
     5641 agaagataaa tcacaagacg acttgaaaga tgatttaaaa gaaaagcaac aatcaagtaa
     5701 ccatcatcaa tccacgcaac ctaagacctc accatcaact gaaacaaaca cgcaacaatc
     5761 atttgctaat tgtaagcaac ttagacaagt atatccgaat ggtgtcactg ccgatcatcc
     5821 agcatatcga ccacatttag atagagataa agataaacgt gcatgtgaac ctgataaata
     5881 ttaaacaaca agcgaattga attcaaattg tatttagctt tatgcactaa tcacatagta
     5941 aataatgagg gagatttttt aggcatgagc aatcaattca aaagcgaaga agagcgaaga
     6001 caatgggaac aattccaagc tttccaaaat caacaaaacc aacagaacca gcaatacgga
     6061 caaaagaaat ctaaaaaagg atggttctgg ggctgtggtg gttgtctagt attatttatt
     6121 ttaattatca tcggtatttc agcttgtaca gctggtatta caggtaacct tggcggaaat
     6181 agttctaaag aaacgaacaa aacccataaa atcggtgaaa ctgttaaaaa tggcgacctt
     6241 gaagtcactg taaattcagt ggaaactatg aaatctgtag gaccatctct tgcaccaaca
     6301 aacgctaaag gtatatttgt cgttgctgat gtgacgatta aaaacaaagg taaagaagcg
     6361 ttaacaattg atagttcaat gtttaagcta aaatccggtg ataaaacatt tgaagcagat
     6421 aatacaggtt caatgtctgc taatcaaagt gacaatggta gtatagaaaa ttcatttttc
     6481 ttacagcgta taaatccaga tagcactgct caaggtaaaa ttgttttcga tgtgtcagaa
     6541 aacatagcca acgcaaaaga taaaaaatta gaagttattt ctagtttatt tagcgtcaag
     6601 aagattacat ttgatttatc cgatgctaaa aaaacatcaa aagctaaaaa agacaagcaa
     6661 gatacagaag tagctgttgc gagttcaaat agcgataatg taagttatga agcttcggct
     6721 actacacctg ctacaacttc tagtgcggat actgattctg aagatagcga aaagtctagt
     6781 aaagatgagg ataagcagaa tgcgtctaaa agtgataaat ctagtgtaga aaaaagtgaa
     6841 tctaatgagg aaactgctcc tgtagagccc atgccccata gcaaacctac cactagtgaa
     6901 gcaccaccta gccaaaatat tcacaatgaa gatagcatgt acgacgcttc aacagaataa
     6961 aatttcaggg tagctcggct acccttcttt tacggaaaaa ttaattatac ataatcaaat
     7021 caaggagata aaaaaatgaa attcaaagct atcgttgcaa tcacattatc attgtcacta
     7081 ttaactgcct gtggtgctaa tcaacataaa gaaaatagta gtaaatcaaa tgacactaat
     7141 aaaaagacgc aacaaactga caacactaca cagtcaaata cagaaaagta aatgacacca
     7201 caagaagccg aagatatagt tcgaaacgat tacaaagcaa gaggcgttaa tgaatatcaa
     7261 acattaaatt ataaaacaaa tcttgaacga agcaatgaac atgaatatta tgttgaacat
     7321 ctagtccgcg atgcagttgg cacaccatta aaacgttgtg ctattgttaa tcgacacaat
     7381 ggcacaatta ttaatatttt tgatgatatg tcagaaaaag acaaagaaga atttgaagca
     7441 tttaaaaaga gaagccctaa atacaatcca ggtatgaaca atcatgatga aacagatggt
     7501 gagtcagaag acattcaaca tcatgacatt gataataaca aagcaattca aaatgacata
     7561 ccagatcaaa aagtcgacga taaaaatgat aaaaatgctg ttaataaaga agaaaaacat
     7621 gataatgggg caaataattc tgaagaaact aaagttaaat aatggcatac tttgattaat
     7681 cgtaattttt aggagaaacg ctatgataac atttgagaat atacaacaac ttgagaaata
     7741 caccttaatg actatgcatg gtctttttaa tcaacttaaa ctcggcatta tttcaatcga
     7801 aaacgcagag catacgcttt ttacacctta tatgttggaa acgctctctt ccctaggcgt
     7861 gaaagacagc attgtcgatt taattcataa agggactgaa ttagaagact ttgcggcatt
     7921 taatttatca attgaagaca cagttacagt ctgtttacaa agaactgaag aactattaaa
     7981 acaatacaaa aatgtggaat tcaatgacaa aatattaatc aattggcgta ttatacaaga
     8041 gaaatagaca tataaaagtc gaatgtaact acgtgagtat tgattttatt ctttgtaaat
     8101 tacaagcatt tcatattata aagtttgaaa agaggtatat tgaaatggag aaaaatgaat
     8161 atatagctaa atataatgaa tatagtcaat tattagacgc tacatactcg caagctgtag
     8221 catacctttt aagtaaatat ggcgctgtaa ccgatgatta ttataaagaa aaatcataca
     8281 cgcgattttt aaatggagaa atcaaaagta tttcaaaagg aaaatacact agagctagcg
     8341 aaggattata ttgccatcat ataagcgagg acaaattcca aaatctatct gatctaagat
     8401 tcatttccaa atttaagtac tcatacgacg ttcaaaagaa agaaaactta gtgtactgtg
     8461 atctaatcga gcatttaatt ttacatgcaa ttattacaaa agaatcccat ggccaatttg
     8521 gtgtagctgg attatgtcaa atgatcaaac caacagtcat tgattggtac attggcgaat
     8581 ataatccaaa accagcatgg atgcaagcca ccaaagcacg tgcctatttg cctggaatat
     8641 tagtagagaa attactcatt aaaattgacg atatgttaaa aggaatagaa atataagatt
     8701 tccttgagtc tagataaatg attaatgtag atttattttt tgctgttgag attttgttat
     8761 agatgtttaa acctgtaatt aaatatattt tataaaatag accacgcata cctatctata
     8821 aacggacaat gtttataaat gagtttgcat ggtcttgaat tgtattaaat ttcttttggt
     8881 tttaataaat cgactagatt ttcacaatat ttatcaaata tgtattccta aattatacag
     8941 ccttaatcca gcagctactt tcgaaacttc caacttagtt gatataaggt tcaatagttt
     9001 gtttcgttct ttttcagata aaccagaact taaattgata ttattgactt cataaaaatt
     9061 atagactaat gcctctattt gctttttagg cataagtaag tcgactgaaa actgatttac
     9121 gtcgctttca taaatcattt catgtaaatt ctttagacta ttatcgttac tatctctcat
     9181 taagtctgta tttttaaata aataacggcc caattcacga gctattgaaa atcttgtatt
     9241 attaatcgag tgattattat taatatagat tgttcttcca cttaaataac ccgaagtatt
     9301 accctccatt ttaatatatc taacatttaa attaagttga aataatagct tgtctatgtc
     9361 aatagcaaag tgttcagaag taataaaaag ttgatccatt ttgtccttta taaatgcctg
     9421 aaataatcga actatttttg attctaaaat atcttcataa tgaactttct caataacttt
     9481 caattgattc cccaccctaa ctaaaaaaat cgaacctaca tcatttaata aatttataac
     9541 tataggtgta aacattatta a
//
LOCUS       DAKTIP010000099         9457 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAKTIP010000099 DAKTIP010000000
VERSION     DAKTIP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.099"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            238..1464
                     /locus_tag="O0305_001712"
     CDS             238..1464
                     /locus_tag="O0305_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274856.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAEISGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDAIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDQQRLVGLITRANVVDIVYDTIWGDSEDTVQTEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            1461..2096
                     /locus_tag="O0305_001713"
     CDS             1461..2096
                     /locus_tag="O0305_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274857.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            2113..3054
                     /locus_tag="O0305_001714"
     CDS             2113..3054
                     /locus_tag="O0305_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0274858.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKNPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            3054..3749
                     /locus_tag="O0305_001715"
     CDS             3054..3749
                     /locus_tag="O0305_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274859.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            4043..5443
                     /locus_tag="O0305_001716"
     CDS             4043..5443
                     /locus_tag="O0305_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274860.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            5669..6604
                     /locus_tag="O0305_001717"
     CDS             5669..6604
                     /locus_tag="O0305_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCY0274861.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            6926..7777
                     /locus_tag="O0305_001718"
     CDS             6926..7777
                     /locus_tag="O0305_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCY0274862.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYQTWK
                     DGMNPIK"
     gene            complement(7869..9365)
                     /locus_tag="O0305_001719"
     CDS             complement(7869..9365)
                     /locus_tag="O0305_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCY0274863.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
BASE COUNT         3103 a         1307 c         1895 g         3152 t
ORIGIN      
        1 tttaaatgtc gaatatacga atgtgcaaac aaagtaatcg gtagaaattc aacatacata
       61 gcgccgttta ctgttaagta ttcacattac agatgaaaaa tataaaattc tacataatca
      121 agaccatgat gtgtacttgt ttaacttatg actctatttg tttaacaatt gcgataatgg
      181 tctttttatt ttatgcgtat cattcgtcat attttttatg aggaaggaga aatgattatg
      241 ttaagtatta agcatttaac gaaaatttat tctggtaata aaaaggcagt agatgacatc
      301 tctttagata ttcaatctgg ggaatttatc gcatttattg gaaccagtgg aagtggcaaa
      361 acgactgctt taagaatgat aaaccgtatg attgaagcga cagaaggaca aattgaaatt
      421 gatggtaaag atgttcggag tatgaatcct gtcgaattgc gtagaaatat tggctatgtt
      481 attcaacaaa ttggcttaat gcctcatatg acgattaaag agaatattgt gttggtaccc
      541 aaattgttga aatggactaa agaggaaaag gataaacgtg caaaggaatt aattaaactt
      601 gtggatttac cggagtcatt tttagagcgt tatccagcag aaatatcagg tgggcaacaa
      661 caacgtatcg gtgttgtaag agcacttgcg gccgaacaag atattatttt aatggatgaa
      721 ccttttggtg cattggatcc tattacgaga gatacgttac aagatttagt taaaacgtta
      781 caacgaaaat taggcaagac gtttatcttt gtaacacatg atatggatga agcgattaaa
      841 ttagcagaca aaatttgtat tatgtcagaa ggtaaggtgg tgcaatttga tacgccagac
      901 aatattttaa gacatcccgc aaatgatttt gtacgtgatt ttataggaca aaatagactg
      961 attcaagacc gtcccaatga caagactgta gaaggtgtaa tgattaaacc aatcacgata
     1021 caagcagaag caacactgaa tgacgccgtt catattatga gacaaaaacg tgttgatgct
     1081 atttttgtag tagatagtaa taaccattta ctaggtttct tagacattga agatataaat
     1141 cagggtatac gtggacacaa aagtttacga gacaccatgc aacaacatat ttataccgtt
     1201 caaattgatt ctaaattaca agattctgta cgtacgattt taaaaagaaa cgttaggaat
     1261 gtacctgtcg tagatgatca acagcgttta gtaggactga ttacgcgtgc caatgttgtt
     1321 gatattgtat atgacacgat ttggggcgat agtgaggata cagtgcaaac agaacatgtg
     1381 ggggaagaca ctgcgtcctc aaaagtgcat gagcaacaca ctactaatgt caaagtacgt
     1441 gacataggag atgataaatc atgattgagt tcctacatga acatggtgga cagttgatgt
     1501 cgaaaacact ggaacatttc tatatttcta tagtggcatt attacttgcc atcattgttg
     1561 cagtacctat aggcatttta ttatcaaaaa caaagcgaac tgccaatatt gtattaactg
     1621 tggcaggtgt cttacaaact attccaacac tagctgtact tgctattatg ataccgattt
     1681 ttggtgttgg taaaacgcct gcaattgtag cgctatttat ttatgtatta ttacctattt
     1741 taaataacac ggtactcggt gttcaaaata ttgatagcaa cattaaagaa gctggaaaaa
     1801 gtatgggaat gacacaattt caattgatga aggatgttga attgccgtta gcattgccgc
     1861 ttatcattgg tggcattcgt ttgtcatctg tgtatgtaat tagttgggct acacttgcaa
     1921 gttatgtagg tgcgggtgga ttaggtgatt tcattttcaa tggtttaaat ttatatgatc
     1981 cactgatgat tgtaactgca acggtactcg ttactgcact agcattaggt gttgatgcct
     2041 tattagcttt agttgaaaaa tgggtagttc ccaaaggctt aaaagtatct ggataattag
     2101 gaggctaaga taatgaagaa aattaaatat atacttgtcg tgtttgtctt atcgcttacc
     2161 gtattatctg gatgtagttt gcccggacta ggtagtaaga gcacgaaaaa tgatgtcaaa
     2221 attacagcat tatcaacaag cgaatcgcaa attatttcac atatgttacg gttgttaata
     2281 gagcatgata cacacggtaa gataaagcca acattagtaa ataatttagg gtcaagtacg
     2341 attcaacata atgccttaat taatggggat gctaatatat caggtgttag atataatggc
     2401 acagatttaa cgggagcttt gaaggaagca ccaattaaaa atcctaagaa agcaatgata
     2461 gcaacacaac aaggatttaa aaagaaattt gatcaaacgt tttttgattc gtatggtttt
     2521 gcgaatacgt atgcattcat ggtaacgaag gaaaccgcta aaaaatatca tttagagaca
     2581 gtttcagatt tagcaaagca tagtaaagat ttacgtttag gtatggatag ttcatggatg
     2641 aatcgtaaag gcgatggcta tgaaggattt aaaaaagagt atggttttga ctttggtaca
     2701 gtgagaccaa tgcaaatagg tctagtctac gacgcattaa actcagagaa gttagacgtt
     2761 gcattaggtt attctacaga tggtcgaatt gcggcgtatg atttgaaagt acttaaagat
     2821 gataaacaat ttttcccacc ttatgctgcg agtgctgttg caacaaatga attattacgg
     2881 caacacccag aacttaaaac gacgattaat aagttgacag gaaagatttc gacttcagag
     2941 atgcaacgct tgaattatga agcggatggt aaaggtaaag aacctgctgt cgtcgcagaa
     3001 gaatttttga aaaaacacca ctattttgat aaacagaaag gtggtcataa gtaatggaag
     3061 gtaatttatt acagcaatta ttcaattatt atgttacgaa ctttggttat ctatgggatt
     3121 tatttttcaa acacttatta atgtctgtct atggtgtgct gtttgcagct ttaattggta
     3181 ttccattggg aatcttgctt gcaagataca caaaactttc tggatttgta attacaattg
     3241 caaatataat tcaaacagtt ccagtcattg caatgttagc tattttaatg ttagtcatgg
     3301 gcttaggttc agaaacagta gttttaacag tgtttttata tgcgttactt ccaattataa
     3361 aaaacactta tactggtata gctagtgttg atgcgaatat taaggatgct ggcaaaggta
     3421 tgggaatgac acgcaatcaa gtgctacgaa tgattgaatt accgttatct gtttcggtta
     3481 ttatcggtgg cattcgtatt gccttggttg ttgcgatagg tgttgttgcc gttggatcat
     3541 ttataggagc acctacgctt ggtgacattg tgattcgtgg tacaaatgcg acggatggca
     3601 caacgtttat tttagcaggt gcgattccga ttgctatcat tgcaatcgtc attgatgtac
     3661 tattaagatt tttagaaaaa cgattagacc caacaacacg acatcgtaaa aatcaatcta
     3721 atcatcggcc gcaaagtatt aatatgtaat agtagaagat gtttataatt tagcgatttc
     3781 gtttcatgat ttataaaaaa tgaggctact caaggagctc aaataatctt tgagtagcct
     3841 ttttataggt tgtgtttgta tgcgtttaca ctaaaatagc aattattatc atgaaagttt
     3901 ttggataaaa agcgttaatt attgtaaaaa tactaaaaaa tgagatgttt tatttataat
     3961 tttctgcaaa tttatgatat tgtttcttaa tatatcatat taaaaatttg tttttcttaa
     4021 acataggagg cttatctaat tcatggacac atcaaaacaa tttagaggtg acaaccgatt
     4081 gcttttgggt atcgttttag gggttattac cttttggcta ttcgcgcagt cacttgttaa
     4141 tcttgttgtc ccattacaat caacatatag tagtgacgtt ggaacgataa atatcgctgt
     4201 tagcttatct gccttatttg ctggtttgtt tatcgtaggt gctggtgatg ttgctgataa
     4261 atttggtcgc gtcaaaatta cttatgtagg attgatatta aatgttgtag gttcattact
     4321 catcatcatt acacctttgc cagcattttt aattataggt agaataattc aaggtttgtc
     4381 tgcagcatgt attatgccat caacacttgc tattattaac gaatattata ttggtacaag
     4441 aagacaacgt gccttaagct attggtctat tggttcttgg ggtggtagtg gtatttgtac
     4501 gttgtttggt ggcttaatgg ctacatatat aggttggcgt tcaatatttg ttgtttcaat
     4561 tctattaaca ttattagcaa tgtacttaat caaacatgca cctgagacta aagcagaacc
     4621 aatcaaaggt atgaaagcag aagctaaaaa gtttgacgtt attggtttag tcattttagt
     4681 agtgacgatg ttaagtttaa atgtaatcat cacacagacg tctcattttg gtttagtttc
     4741 accgttaatt ctaggtttaa ttgttgtgtt tatctgttca ttagttggtt ttgtatatta
     4801 cgaaaataaa ataaagcatc cacttgttga tttttcaatt tttaaaaata gaggatacag
     4861 tggtgcaact atttcaaact tcttattaaa tggtgtagca ggtggtgcac ttatcgttat
     4921 taacacgtat tatcaacaac aattaggatt taattcttcg caaacgggtt atatttcatt
     4981 aacgtattta ataacagtgt tgtcaatgat tcgtgtaggt gaaaagattt tatctcaaca
     5041 tggtccgaag cgcccactat tactaggaag tggctttaca gtgattgggt taatcttatt
     5101 gtcgttaaca tttttaccag aagtgtggta tatcatatct agtatagttg gatatttatt
     5161 gtttggtact ggtttaggat tatatgctac accatcaact gatacagcag ttgctagtgc
     5221 gccagatgat aagtcgggtg ttgcttcagg tgtgtataaa atggcgtcat cattaggaaa
     5281 tgcatttgga gtagcagtat ctggtacggt ttatactgtg ttagcagcta atttaaattt
     5341 gaacttaggt ggtttcacag gtatgatgtt taatgccttg ctagcaattg ttgcattttt
     5401 agtcatttta ctattagttc ctaaaaatca aacgaatttg taaaactgaa atgaaagcaa
     5461 gttattatgt agggatttta aaggaaattt tgtgaaagta agtttatcat acacacttaa
     5521 tgttgcgtat tgacgtttaa tgttaggtgt gttcttttat agacgataaa agctgtgtgc
     5581 atattaagcg aatgattttc aaattgacgc taatatgcga aagtagtatt tttaaaatga
     5641 acaacaacga tgaagagggg tttataggat gaaaattgca attgctggat cgggtgcatt
     5701 aggtagtggc tttggtgcca aactatttca agcaggatat gatgtcacac ttattgacgg
     5761 atatacatct catgttgaag cggttaagca acatggatta aatataacga ttaatggaga
     5821 ggcattcgag ttaaacattc cgatgtatca ttttaatgat caaccggacg aaagcattta
     5881 cgatgttgtc tttctatttc caaagtctat gcaattaaaa gaagtgatgg aagatatgaa
     5941 gccacatatt gataatgaaa cgatcgtcgt atgtacgatg aatggtctga agcatgaaga
     6001 agtcattgcg cagtatgttg ctcaatcaca aattgtcaga ggtgttacga cttggacggc
     6061 aggtcttgaa agccctggac acagtcattt acttggtagt ggaccagttg aaataggtga
     6121 actagtggat gaaggtaaag aaaatgttat aaaagttgct gatttactta acgaagcgga
     6181 attgaatggt gtcattagta aagatttata ccaatcgatt tggaaaaaga tttgtgttaa
     6241 tggtacggca aatgcattaa gcacagtgtt ggagtgtaat atggcatcgc tgaatgaaag
     6301 tagttatgcg aagtgtttga tttataaatt aacgcaagaa atagtgcatg tagcgacgat
     6361 tgataatgtt catttaaatg ttgatgaagt atttgaatat ttagttgatt taaatgaaaa
     6421 agttggtgcg cattatccat ccatgtatca agatttaatt gttaataata gaaaaactga
     6481 aattgattat attaatggcg cagttgcaac attaggtaaa caacgtcata ttgaagcgcc
     6541 agtcaatcgc tttattactg atttaattca tactaaagaa agtcagcgac acgcacagga
     6601 ttaattggtg ttttaggtat gattacaagg aaatgacaat cacgtgatat tacggtcatt
     6661 attaagattg aaatgtaata aataaagaac agcagtaagg tactttcaaa ttgaaatgat
     6721 cttggtgctg tttttcttga ttgatcttcg tcataattca gatttgtcat aggtctacga
     6781 catactatta gtatttacta gacagttttt acgacgacac tttgaaaaat tttgaggcaa
     6841 atcatttgga agtctcacgt gaattttgta aactcatcaa gcaagtaatt atattaaaaa
     6901 gacaaataga gaaaaggtgt ttataatgag taaaattttt gtaactggtg caacgggcct
     6961 tattggcatt aaattagttc aaagactaaa agaagagggg catgaggttg ctggttttac
     7021 tacatctgag aatggtcaac aaaagctagc tgctgttaat gtaaaagcat atattggtga
     7081 tatattaaaa gctgatacta ttgatcaagc gttagcagat tttaaaccag aaatcattat
     7141 caatcaaatt acggatttaa aaaatgttga tatggcagca aatacgaaag tacgtattga
     7201 aggttctaaa aacctaattg atgcggcgaa aaagcatgac gttaagaaag taattgccca
     7261 aagtattgcc tttatgtatg aacctggcga aggattagca aatgaggaaa cttcacttga
     7321 ttttaactca actggcgata gaaaagtaac ggttgatggt gtggttggtt tagaagaaga
     7381 aacggctcgt atggatgaat acgttgtttt acgttttggc tggttatatg gcccaggtac
     7441 ttggtacgga aaagatggca tgatttataa tcaatttatg gatggtcaag tgacactttc
     7501 agatggcgta acatcatttg tgcatcttga tgatgcagtt gaaacatcta ttcaagctat
     7561 tcattttgaa aatggtatct ataatgtagc agatgatgca cctgttaaag gttctgaatt
     7621 tgcagaatgg tataaagaac aacttggtgt tgaaccaaat attgatattc aacctgcgca
     7681 accatttgaa cgtggcgtaa gcaatgagaa gtttaaagcg caaggtggta ctctgattta
     7741 tcaaacttgg aaagatggca tgaatccaat taaataataa tttatccgtt taatatacaa
     7801 agaataaaga cttggtcgaa tcgtggatga tatattatca aacgcacggc tcgaacaagt
     7861 cttttttatt atgtcttcgt tatctttgta tgaaggaata acagaattac aattaatgta
     7921 ctgaataatg caattaatgt tgtgattagt gctaatttaa tttctattgg tagccaagtc
     7981 agtacaaaag accaattatt gctaccgaga atgagatatg gtaatgcata taatatgagc
     8041 gctaaagcga tacatataca taatgataac caactcaata cagcaatccg ttttgatcta
     8101 cgcaaaagaa tttgaccatg gcgcatttgc caagcacgat aagctgagaa tataaatgct
     8161 aataaaataa gtgttgtcat caaaacggta acaatattaa attgatcttt atattgattg
     8221 agcatagacg caaccgtcga atatcgcttg tgatttacaa tttgtgtatt aagatgctca
     8281 actaatttgg gtacatattc cgaatttaga tttgcaagta caacaattcc ataattttgt
     8341 tttggattta gtaaaataaa tgatgaaaag ttatctagcg ttcctgaatg aaacactaaa
     8401 tgttcatcat tattggtaaa ccagccggaa gcatatgcat tggcattagg ttcaccaatt
     8461 gttgaagata aatttttatg tgattgttga actaatgatt tgtatttatc aggtggatta
     8521 agttggaatt ttatccaatg ttccaaatct tcagttgatg tcatcatata tgctgatggt
     8581 gtatcccaaa ggttaaattc aggtttagag acgacaggtg tcgaaccttg taattcatag
     8641 ccaatagcat catgttttga tttgtaattg gtttgtttga atgatgtatg tgtcatatgc
     8701 aaaggcttga gccatgaatt tgtaatatat tttgtatagg attgcttcgt aacgttttgg
     8761 ataattaaac ctaataaatc atagttcata tttgagtatt caaattcttc tccgggctta
     8821 tgatgtaatt catcacccat aattgcacgg gttacatcat ttaaacgatt atttttgctt
     8881 gtcacagaat cttcgcttgt aatatcacta ggtataccac ttgtttgagc caaaagttgc
     8941 ttaatcgtaa tagtttcatt ttgaccatta tagttcattt taaaatgagg cacatgtttg
     9001 gatacggcat catttaagtt taatcgacct tcttgagcta attttaaaat tgcaagacct
     9061 gtgaaagctt tcgtattaga agcaatttca tactttgttg tgggagaagc tttgactttt
     9121 ttatcaacat cttgataacc ataaccttta tttaaaaaaa ctttgccatt ttttactatt
     9181 aaaattgatg ctccaggaat gtgtcccttt tgtaaatcat gctcgataat tgtatctatt
     9241 tgttgttgcg aatcattggt taaccgtgtc ttcgtattgc tatttaatgt tatatatata
     9301 gcaattgaaa tggcgactaa aatgataata gaaatggata gaaaatacag ttttttagta
     9361 gtcataaacg ttccttttta tatgttaaat ttacttaagt gcctaatgag atttatttta
     9421 ttataccagt tctattgaat aaattaatta aagaggt
//
LOCUS       DAKTIP010000100         9438 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.100, whole genome shotgun sequence.
ACCESSION   DAKTIP010000100 DAKTIP010000000
VERSION     DAKTIP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.100"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            60..1085
                     /locus_tag="O0305_001720"
     CDS             60..1085
                     /locus_tag="O0305_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCY0274864.1"
                     /translation="MNNKVLATGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDS
                     MAKYHLI"
     gene            complement(1178..2524)
                     /locus_tag="O0305_001721"
     CDS             complement(1178..2524)
                     /locus_tag="O0305_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCY0274865.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAIAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATTILLKGRQKEQQ"
     gene            complement(2539..2823)
                     /locus_tag="O0305_001722"
     CDS             complement(2539..2823)
                     /locus_tag="O0305_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCY0274866.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            complement(2825..3268)
                     /locus_tag="O0305_001723"
     CDS             complement(2825..3268)
                     /locus_tag="O0305_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCY0274867.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            complement(3273..5228)
                     /locus_tag="O0305_001724"
     CDS             complement(3273..5228)
                     /locus_tag="O0305_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCY0274868.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLIKDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            5440..5859
                     /gene="mepR"
                     /locus_tag="O0305_001725"
     CDS             5440..5859
                     /gene="mepR"
                     /locus_tag="O0305_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCY0274869.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            5966..7321
                     /gene="mepA"
                     /locus_tag="O0305_001726"
     CDS             5966..7321
                     /gene="mepA"
                     /locus_tag="O0305_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCY0274870.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            7425..7865
                     /locus_tag="O0305_001727"
     CDS             7425..7865
                     /locus_tag="O0305_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCY0274871.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILASSKYQGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(7948..9306)
                     /gene="glpT"
                     /locus_tag="O0305_001728"
     CDS             complement(7948..9306)
                     /gene="glpT"
                     /locus_tag="O0305_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCY0274872.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
BASE COUNT         3131 a         1513 c         1750 g         3044 t
ORIGIN      
        1 tacaaaatat aatgactgct ataatgagta atcaatagac acaaagagga gattatgtga
       61 tgaataataa agtattagca accggtggta cagggtttgt tggcatgcga attatttcac
      121 gattattaga acaaggttat gacgtacaaa cgacgatacg tgatttaagt aaagctgata
      181 aagtaattaa aacaatgcaa gacaacggca tttccacaga gcgattaatg tttgtcgaag
      241 cggatttatc acaagatgaa cattgggatg aagcaatgaa agattgcaag tatgtcttga
      301 gtgtagcatc tccggtgttt ttcggtaaaa cagacgatgc agaagtgatg gcgaagcctg
      361 caattgaagg tatacaacgt attttaagag ctgcagaaca tgcgggtgtt aaacgtgtgg
      421 taatgactgc aaactttggt gcagttggtt ttagtaataa agataaaaat tcaatcacaa
      481 atgaaagtca ttggacaaat gaagatgaac caggcttatc agtatatgaa aaatcaaaat
      541 tgttagctga aaaggcagcg tgggattttg ttgagaatga aaatacaaca gtagaatttg
      601 ccacaatcaa tccagttgca atttttgggc catcattaga tgcacacgtt tcaggaagct
      661 ttcatttatt agaaaattta ttgaatggtt caatgaaacg tgtaccgcaa attccgttaa
      721 atgttgttga tgtgagagac gtagctgaac tgcacatttt ggcaatgaca aatgaacaag
      781 ctaatggcaa gcgatttatt gcgacggctg atggacaaat taatttgttg gaaattgcaa
      841 aattaattaa agaaaagaga cctgaaatag ctcaaaaagt ttctactaaa aaattaccag
      901 actttatttt gagtctaggt gctaaattta atcatcaagc taaagaaggt aaacttttat
      961 tagatatgaa ccgcaatgta agtaacgaac gtgcaaaaac attacttggt tgggaaccga
     1021 ttgcgacaca agaagaagca attttagcag ctgtcgatag tatggctaag tatcatttaa
     1081 tataatacaa aacaccgtcc ataagtaagt ggctttcctc ttgataaaag gggcattcac
     1141 tcaatatgtg acggtgtttt atatttctac gttaacacta ttgctgttct ttttgacggc
     1201 cttttaataa aattgttgtc gcacctacga taataataaa tagaatcgca ccaaataatc
     1261 ccatatattt tactgcgtta ccgaacacga taccgacagc taaaaagtct gtatctgaga
     1321 atgttgttgc agcaccacct aattcgccta aaaatggcaa gaataataat ggtaaaaacg
     1381 tgattaggat accatttaga gcggcgccag caatagcacc tttaataccg cctcttgcat
     1441 taccgaatac agcagccgtt gcacctaaga agaagtgtgc aactacgcca ggtaaaatga
     1501 cgacgccacc aaataagaat aagataaaca taccgatgac acctgtaata aagctgacaa
     1561 agaatccaat taatactgca ttttgtgcat aagggaacac aatagggcag tctaatgcag
     1621 gtttagaatt tggtacaagc ttttcagaaa ttcctttaaa tgctgggacg atttcagcta
     1681 agattaaacg aacgcccgtt aaaataataa atacaccagc agcaaatgtc acaccttgaa
     1741 ttaatgaaaa gacaataaag ttttgaccat cactaataga ttcgtgtaca taactaacgc
     1801 ccgcaaataa gcatgcgatg aagtaaagta atgccatcgt aatcgagata ctaattgtac
     1861 tttctcgtaa gaaacttaag ccttttggaa atttaatctc ttccgttgat ttagacttac
     1921 ctttgaataa ttgacctaca gcacctgcgg caaagtaact gattgagcca aaatgaccta
     1981 aagctacttg gtcattccct gtaatttttc gcatcgtagg ttggagtaat gcaggtaata
     2041 ctgccatgat taatcctaat acgagtgcgc cgataacaat cgttagccag cctttaatat
     2101 gactgactgt taaaatgatt gctaaaaacg cagccatgta aaatgtatga tgacctgtta
     2161 aaaagatata ttttaaatta gtgaagcggg caattaaaat attaacaatc atgccacaga
     2221 ccatgatgag tgcagctgtt gttccaaaat cttttaaggc tagtgagacg atagcttcgt
     2281 tgttaggtac gataccttgc acaccaaatg cgtgttggaa tattttgccg aatggttcaa
     2341 gagatcgaac gacgacatca gcacctgcac ttaaaattaa gaagcctaat atcgttttaa
     2401 tggttcctga agtgatcgtt gcggcaggtt ttttctgaac gattaaacct ataaaggcaa
     2461 tcagtgcaac aagaatggct ggttgactta aaatatcgac tataaaatta aggattgctt
     2521 gcataggtcg tacctccttt aaatcatgtt aagttgttgt aatttttctg agagcttttg
     2581 ttgtaattca gctttatcta aaatattatc aagaactaag acatccccta gacgttcggc
     2641 attttcagct aaatctctac cacaaataaa caagtcagcc atctctggac ttgctgtcat
     2701 aatgtcacta tgttcaactt cgatatcaga tggtgcatta agttgcctaa gtgcttcttg
     2761 tgcgttcatt tctaccataa aactacttcc taaaccgtgg ccacatacta ctaaaatttt
     2821 catattaatc atgctccttt aaaatgtttt taatgtcttg tgcatttgtt gcagttaata
     2881 gttgctggac tgtttggtta tcgcccagta cggttgctaa attttgtaat acagataagt
     2941 gtgaatgatt gtcgatggca ctcaatacaa aaatgagaga tgcgtagtga tcttcatcac
     3001 aaaatgccac atgttgattc aactttaata gacttaaacc aacttgatgt acgtcattgt
     3061 tcggtcttgc atgtgcaatt gcaatttcag gtgcgataac gatataaggt ccaagttcat
     3121 taacgctatc aatcattgct tgaacatagc cttgttcaat aatttgttct tgtagtaatg
     3181 gctgagaagc tatagttata gcttcagtcc aatcatttac ttgttctttt acaatgatgc
     3241 gtgttgttga caaaatgtct aatgacacgt tattaagcct cctttgtcat agttaaagca
     3301 atgtgttgtt taattttaaa aatattccca tctaagaaat cttgtcgata taagtcgttg
     3361 cttaagcatt cgcttaactg tcccaatgcc tttaaatgtg cattggggtg gtccgttgct
     3421 aatgtaatta caaggtgaac gggatcgtta gctttactac caaagataat cccttcagtg
     3481 aaatatgtta gtgcgaaacc tacaccattc tgtacataat cagtaccagc gtgaataagt
     3541 gcaatatgtg gactaatgac catatatgac ccgaattgtt caaattgttt taaaattgca
     3601 gctgtataat ttgaatagac aatgccatct ttgattaaag gttgcacagc cactgcaatt
     3661 gcggattcaa ttgataatgg ttgtttattt ataatgatgc gatgttcagg caataaatct
     3721 gcgagtgact tgccatcagt tgccattttc atgactcgtt gttctcttga gtcattgata
     3781 atttgattca atttttgacg agattgttga ttgataaatg gatcgacatg aataactggt
     3841 acagctgata tttcacaagg tactgttgaa atgacataat caatgttatc ttgcaataat
     3901 cgactttctt ccaattgata aatggaatag gcatcccaaa tgtgaaactc aggatacagg
     3961 tgatttagtt ttgattttaa aagttgtgac gtgcctatac cagaaccaca tagtaagaca
     4021 accttaatca ttgattgttt atgtgttgca acacgctcta tacttgatgc gaagtgaatt
     4081 gtaatgtatg ttaattcatc ttcgttgaag cgaatagcag catcttgttc aattggacta
     4141 atatgcttgc taacggcttc aatgatttga ggatagcgac gcataacttc ttgcctcaaa
     4201 ggattaggtt gtagcatatc gtatttaata cgatgtatag ctggtttgat atgtgtgatc
     4261 agactggtat gtaacttgtt gtcttttgac atatcaatgc ctaattcttg gctaacacaa
     4321 gtgatcaatt catgtatatt ttgcgataaa tcatggtatt caaaggtaat tgaagatgct
     4381 gtatgttcag tcattttaga gcctagtaaa tgtaacgtga taaagataat ttcagactct
     4441 ggaaatgtga cattacaact gcgttctaag ttttctatca tttttgaagc aatagcatac
     4501 tgattagtat gtcgccattt atcaatttca ttgataggta tatcgaacga aaaattttca
     4561 tttaaacgct gaatggcaat gagtatatga tagattaagc catcgatagc cgactgaact
     4621 aaatgataat tttcactatt taatgtctta ataatggcac ggcgaaccaa tgcgattgat
     4681 tctgaattaa agatatccgc ctctataaaa ggtgcagctt gtttcatata ttgatgtata
     4741 aagtgtgcat acgctttacg ataatgatct tcctcaccaa taatattgaa tcctttattg
     4801 tggacataat ttaactttaa atggtattga tctagttggg cttgaatcat tttaatatca
     4861 tctgcaattg tccgacgcga aacattaaca tcttgcgcaa gttgctttgt tgaaacagga
     4921 tcggttgttt cgaataactt taaagcgata tgtgtgagtc gttcatcttt tgaaaaatga
     4981 atttgatttg ttagattgtg ctctaattca tttaatagtg tcgcgtgagc tgttgttact
     5041 ttgatgcctg cagctttatt acggctgact tggtaatgat aagtttcagc atattgctca
     5101 atatatgcta tatcatattg aatggtacga ggtgatacac caagttgatt agcaatggta
     5161 ttgattggaa taaacgtttg ctcatgaatt aaaagataca aaatttcgat ttgtctataa
     5221 cttaacaacg taatatcctc ctatttgtaa ttgtaagcga tttcttaaaa acgtagatat
     5281 gcaatctctt tcatatttta atccgaaaaa ttgcatatca aaatgtttat ggcgcaagat
     5341 tttataggaa cttttaaaat aaattagata ttcatgttgt caatttaaaa atgtcgcagt
     5401 atatttagtt agacatctaa cgaaatggtg gtgcaataaa tggaattcac ttattcgtat
     5461 ttatttagaa tgattagtca tgagatgaaa caaaaggctg atcaaaagtt agagcaattt
     5521 gatattacaa atgagcaagg tcatacgtta ggttatcttt atgcacatca acaagatgga
     5581 ctgacacaaa atgatattgc taaagcatta caacgaacag gtccaactgt cagtaattta
     5641 ttaaggaacc ttgaacgtaa aaagctgatc tatcgctatg tcgatgcaca agatacgaga
     5701 agaaagaata tagggctgac tacctctggg attaaactcg tagaagcatt cacttcgata
     5761 tttgatgaaa tggaacaaac actcgtatcg cagttatctg aagaagaaaa tgaacaaatg
     5821 aaagcaaact taactaaaat gttatctagt ttacaataaa tgataagtgt gactggtaga
     5881 aatcagtcac tttgtcttta atattatagt tagatatcta attgttagta agctaattat
     5941 tggaaaagac aaggagtatt gaacaatgaa agacgaacaa ttatattatt ttgagaaatc
     6001 gccagtattt aaagcgatga tgcatttctc attgccaatg atgataggga ctttattaag
     6061 cgttatttat ggcatattaa atatttactt tataggattt ttagaagata gccacatgat
     6121 ttctgctatc tctctaacac tgccagtatt tgctatctta atggggttag gtaatttatt
     6181 tggcgttggt gcaggaactt atatttcacg tttattaggt gcgaaagact atagtaagag
     6241 taaatttgta agtagtttct ctatttatgg tggtattgca ctaggactta tcgtgatttt
     6301 agttacttta ccattcagtg atcaaatcgc agcaatttta ggggcgagag gtgaaacgtt
     6361 agctttaaca agtaattatt tgaaagtaat gtttttaagt gcaccttttg taattttgtt
     6421 cttcatatta gaacaatttg cacgtgcaat tggggcacca atggtttcta tgattggtat
     6481 gttagctagt gtaggcttaa atattatttt agatccaatt ttaatttttg gttttgattt
     6541 aaacgttgtt ggtgcagctt tgggtactgc aatcagtaat gttgctgctg ctctgttctt
     6601 tatcatttat tttatgaaaa atagtgacgt tgtgtcagtt aatattaaac ttgcgaaacc
     6661 taataaagaa atgctttctg aaatctttaa aatcggtatt cctgcatttt taatgagtat
     6721 cttaatggga ttcacaggat tagttttaaa tttattttta gcacattatg gaaacttcgc
     6781 gattgcaagt tatggtatct catttagact tgtgcaattt ccagaactta ttatcatggg
     6841 attatgtgaa ggtgttgtac cactaattgc atataacttt atggcaaata aaggccgtat
     6901 gaaagacgtt atcaaagcag ttatcatgtc tatcggcgtt atctttgttg tatgtatgag
     6961 tgctgtattt acaattggac atcatatggt cggactattt actactgatc aagccattgt
     7021 tgagatggcg acatttattt tgaaagtaac aatggcatca ttattattaa atggtatagg
     7081 tttcttgttt actggtatgc ttcaagcgac tgggcaaggt cgtggtgcta caattatggc
     7141 cattttacaa ggtgcaatta tcattccagt attatttatt atgaatgctt tgtttggact
     7201 aacaggtgtc atttggtcat tattaattgc tgagtcactt tgtgctttag cagcaatgtt
     7261 aatcgtctat ttattacgtg atcgtttgac agttgataca tctgaattaa tagaaggtta
     7321 aatatttcgt ccacttctgg ctgagtatat ttcggtcgga agtgtatttt tcgaaaaaaa
     7381 taaatatatg atacgattat gaaaaaataa agtgagattg gcatatgtgt aaatctaaaa
     7441 tactgttgaa aaatattttt agtgaagaat cagaagttaa agatttaact gaagaaaaat
     7501 ataatcaaga ttacgaagca ttaacattta gctttaaaga ggaaacatat caaagtaggt
     7561 tagctaagaa aacaccgact aaatcgggat atttcgtgac atgttggaca aaagacgaag
     7621 ataattataa tcggccatac aaaattgaag agtttgctga ttacctgatt gttgctgtta
     7681 tcgatgatga attaaatggc tactttctat ttcctaggga attattggta gaaaaaggta
     7741 tcttagcttc atctaagtat caagggaaaa tggcttttag agtttatcct aagtggtgta
     7801 atcaattgaa taaaacagca gggcaaacac aaaagtggca atgtaaatat ttttttgaat
     7861 actaataaaa agtcatatgg ttgaacttat aagagatgaa tatatcatct ttcgataaga
     7921 taaccatatg actttatttt ttatcattta atgatgaacg gtttcttgtc ctactttatt
     7981 ccaagtgagg ataaagctca acattgcaaa cacactaatt gctgttaata aaataaaacc
     8041 gacatcccat ccgaatttat caactacagc acctaagacg atgttggcca ttacagcacc
     8101 aaacagataa ccaaataatc ctgttaatcc agctgctgtg ccagctgctt ttttaggtac
     8161 ataatctaat gcttgtaaac caattaacat aactggtcca tatattaaga aaccaatggc
     8221 aattaatgag acattgtcta accaagcatt gcctggagga tttaaccaat aaattaatac
     8281 aaatactgtg acacctaaca taaagaagaa acctgcaggt ccacgacgac ctttgaataa
     8341 tttatcagaa atgtaaccac ataataatgt accaggaatt ccagcccatt cgtataagaa
     8401 gtatgcccaa cctgatgctt ttaagtcgaa atgtttttct tcacttaagt agactggcgc
     8461 ccaatcaagt acaccataac gcacgaaata aacaaatata tttgcaaagg caattgccca
     8521 tacccattta ttgttcagta catatttaaa taaaatttct tttgtagtta attctgtttc
     8581 taatgttttc ttatcgcttg tagcaaagtc atttttataa atttcgattg gaggtaaacc
     8641 ttgagattga ggtgtgtctc taatcaatac gtatgaaatt gcggcaatga taagtgctaa
     8701 gagtgcaggg taaatgaata caccttcgaa accttttaaa taaccaaagt tgataaatgc
     8761 agttgttgta ataccccaag cagcaatagg tgccataata cctccaccaa cattatgcgc
     8821 aacgttccaa agggcagtct tacttccgcg ttcacttaca ctaaaccagt gaacgagaac
     8881 acggcctgaa ggtggccagc ccataccttg aaaccatcca tttaagaata ataggacaaa
     8941 cataataccg atacctgatg tgaagaacgg tacaaatccc attaacaaat tgacgatagc
     9001 agtgagtgct aatccaagaa ctaagaatat ccgagcattg ctccgatcac ttacagtacc
     9061 cataaagaac ttactaaatc catatgcgat ggaaacagca gaaagtgcaa aacctagttc
     9121 cgcttttgta aaaccttgct cttgcaatgc cggcatcgct aacgaaaagt ttttacgtaa
     9181 taaatagtac ccagcataac cgatgaaaat accaagaaat acttggagac gtaatcgttt
     9241 ataggtatca tctatctgat tttctggcaa aggcttaata tgctttgcag gtttaagaaa
     9301 attcataaaa tcctccttaa tatgtattta tatgcatttt gtgcgataaa cttatattag
     9361 acatgtatga aagcgttgtc aatatgcttt tgtgaaatct tgcatattta gttattgaag
     9421 tattttataa acatttag
//
LOCUS       DAKTIP010000101         9215 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAKTIP010000101 DAKTIP010000000
VERSION     DAKTIP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.101"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            146..754
                     /locus_tag="O0305_001729"
     CDS             146..754
                     /locus_tag="O0305_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0274873.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            969..4520
                     /gene="rpoB"
                     /locus_tag="O0305_001730"
     CDS             969..4520
                     /gene="rpoB"
                     /locus_tag="O0305_001730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCY0274874.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            4684..8280
                     /gene="rpoC"
                     /locus_tag="O0305_001731"
     CDS             4684..8280
                     /gene="rpoC"
                     /locus_tag="O0305_001731"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCY0274875.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIHHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            8417..8671
                     /locus_tag="O0305_001732"
     CDS             8417..8671
                     /locus_tag="O0305_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCY0274876.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            8769..9182
                     /gene="rpsL"
                     /locus_tag="O0305_001733"
     CDS             8769..9182
                     /gene="rpsL"
                     /locus_tag="O0305_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCY0274877.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
BASE COUNT         3202 a         1452 c         1845 g         2716 t
ORIGIN      
        1 ttttatagca agtattgcta taatataatg attctttgag aagttaaaac cccgttattt
       61 tgataacggg gttttattca tttaaagact gagtgaaatg ttataattat aatgacgagt
      121 tacaaagtga agatgaggtg gaataatgag tcattattac gatgaagatc caagtgtaat
      181 tagcaatgaa caacgtattc aatatcaatt aaaccatcat aaaattgatt taataactga
      241 taacggagtg ttttcgaaag ataaagtaga ttatggttca gatgttcttg ttcaaacttt
      301 tttaaaagcg catccacctg gtccaagtaa gcgaattgcc gatgttggtt gtggttacgg
      361 accaattggt ttgatgattg ctaaagtatc accacatcat tcaattacaa tgctagatgt
      421 taatcacaga gcgctagcct tagttgaaaa aaacaaaaaa ttaaatggta ttgataatgt
      481 gatcgtaaag gaaagtgatg ctttgtctgc tgtggaagac aaaagttttg attttatttt
      541 aaccaatcca ccaataagag cagggaaaga aaccgtgcat cgtatattcg agcaagcatt
      601 acatagatta gactcgaacg gtgaactatt cgttgtaatt cagaagaagc aaggtatgcc
      661 atctgcaaag aaaagaatga atgaactttt tggaaatgta gaagtggtaa ataaagataa
      721 aggatattac attctgagaa gtataaaagc ttgaaatgaa atggatattc tgttatagtt
      781 atataatgta aaaatttatg ttcaataagt gtgtactttt acgttaaata gataagttaa
      841 ttaagaataa atatagaatc gaaaatggtg tcatcattag tgttgccgtt ttctttttgt
      901 ctttttatta atatgcttat ggtatttagc taaaagcgga tcacataatt tttgaggggt
      961 gaatctgttt ggcaggtcaa gttgtccaat atggaagaca tcgtaaacgt agaaactacg
     1021 cgagaatttc agaagtatta gaattaccaa acttaataga aattcaaact aaatcttacg
     1081 agtggttcct aagagaaggt ttaatcgaaa tgtttagaga catttctcca attgaagatt
     1141 ttactggtaa tttgtcatta gagtttgtgg attaccgttt aggagaacca aaatatgatt
     1201 tagaagaatc taaaaaccgt gacgctactt atgctgcacc tcttcgtgta aaagtgcgtc
     1261 taatcattaa agaaacagga gaagttaaag aacaagaagt ctttatgggt gatttcccat
     1321 taatgactga tacaggtacg ttcgttatca atggtgcaga acgtgtaatc gtatctcaat
     1381 tagttcgttc accatccgtt tatttcaatg aaaaaatcga caaaaatggt cgtgaaaact
     1441 atgatgcaac aattattcca aaccgtggtg catggttaga atatgaaaca gatgctaaag
     1501 atgttgtata cgtacgtatt gatagaacac gtaaactacc attaacagta ttgttacgtg
     1561 cattaggttt ctcaagcgac caagaaattg ttgacctttt aggtgacaat gaatatttac
     1621 gtaatacttt agagaaagac ggcactgaaa acactgaaca agcgttatta gaaatctatg
     1681 aacgtttacg tccaggtgaa ccaccaactg ttgaaaatgc taaaagtcta ttgtattcac
     1741 gtttctttga tccaaaacgc tatgacttag caagcgtggg tcgttataaa acaaacaaaa
     1801 aattacattt aaaacatcgt ttatttaatc aaaaattagc tgagccaatt gtaaatactg
     1861 aaactggtga aattgtagtt gaagaaggta cagtgcttga tcgtcgtaaa atcgacgaaa
     1921 tcatggatgt acttgaatca aatgcaaaca gcgaagtgtt tgaattgcat ggtagcgtta
     1981 tagacgagcc agtagaaatt caatcaatta aagtatatgt tcctaacgat gatgaaggtc
     2041 gtacgacaac tgtaattggt aatgctttcc ctgactcaga agttaaatgc attacaccag
     2101 cagatatcat tgcttcaatg agttacttct ttaacttatt aagcggtatt ggatatacag
     2161 atgatattga ccatttaggt aaccgtcgtt tacgttctgt aggtgaatta ctacaaaacc
     2221 aattccgtat cggtttatca agaatggaaa gagttgtacg tgaaagaatg tcaattcaag
     2281 atactgagtc tatcacacct caacaattaa ttaatattcg acctgttatt gcatctatta
     2341 aagaattctt tggtagctct caattatcac aattcatgga ccaagcaaac ccattagctg
     2401 agttaacgca taaacgtcgt ctatcagcat taggacctgg tggtttaaca cgtgaacgtg
     2461 ctcaaatgga agtacgtgac gttcactact ctcactatgg ccgtatgtgt ccaattgaaa
     2521 cacctgaggg accaaacatt ggattgatta actcattatc aagttatgca cgtgtaaatg
     2581 aattcggctt tattgaaaca ccatatcgta aagttgattt agatacacat gctatcactg
     2641 atcaaattga ctatttaaca gctgacgaag aagatagcta tgttgtagca caagcaaact
     2701 ctaaattaga tgaaaatggt cgtttcatgg atgatgaagt tgtatgtcgt ttccgtggta
     2761 acaatacagt tatggctaaa gaaaaaatgg attatatgga tgtatcgccg aagcaagttg
     2821 tttcagcagc gacagcatgt attccattct tagaaaatga tgactcaaac cgtgcattga
     2881 tgggtgcgaa catgcaacgt caagcagtgc ctttgatgaa tccagaagca ccatttgttg
     2941 gtacaggtat ggaacacgtt gcagcacgtg attctggtgc ggctattaca gctaagcaca
     3001 gaggtcgtgt tgaacatgtt gaatctaatg aaattcttgt tcgtcgtcta gttgaagaga
     3061 acggcgttga gcatgaaggt gaattagatc gctatccatt agctaaattt aaacgttcaa
     3121 actcaggtac atgttacaac caacgtccaa tcgttgcagt tggagatgtt gttgagtata
     3181 acgagatttt agcagatgga ccatctatgg aattaggaga aatggcatta ggtagaaacg
     3241 tagtagttgg tttcatgact tgggacggtt acaactatga ggatgccgtt atcatgagtg
     3301 aaagacttgt gaaagatgac gtgtatactt ctattcatat tgaagagtat gaatcagaag
     3361 cacgtgatac taagttagga cctgaagaaa tcacaagaga tattcctaat gtttctgaaa
     3421 gtgcacttaa gaacttagac gatcgtggta tcgtttatat tggtgcagaa gtaaaagatg
     3481 gagatatttt agttggtaaa gtaacgccta aaggtgtaac tgagttaact gccgaagaaa
     3541 gattgttaca tgcaatcttt ggtgaaaaag cacgtgaagt tagagatact tcattacgtg
     3601 tacctcacgg cgctggcggt atcgttcttg atgtaaaagt attcaatcgt gaagaaggcg
     3661 acgatacatt atcacctggt gtaaaccaat tagtacgtgt atatatcgtt caaaaacgta
     3721 aaattcatgt tggtgataag atgtgtggtc gacatggtaa caaaggtgtc atttctaaga
     3781 ttgttcctga agaagatatg ccttacttac cagatggacg tccgatcgat atcatgttaa
     3841 atcctcttgg tgtaccatct cgtatgaaca tcggacaagt attagagcta cacttaggta
     3901 tggctgctaa aaatcttggt attcacgttg catcaccagt atttgacggt gcaaacgatg
     3961 acgatgtatg gtcaacaatt gaagaagctg gtatggctcg tgatggtaaa actgtacttt
     4021 atgatggacg tacaggtgaa ccattcgata accgtatttc agtaggtgta atgtacatgt
     4081 tgaaacttgc gcacatggtt gatgataaat tacatgcgcg ttcaacagga ccatattcac
     4141 ttgttacaca acaaccactt ggcggtaaag cgcaattcgg tggacaacgt tttggtgaga
     4201 tggaggtatg ggcacttgaa gcatatggtg ctgcatacac attacaagaa atcttaactt
     4261 acaaatccga tgatacagta ggacgtgtga aaacatacga ggctattgtt aaaggtgaaa
     4321 acatctctag accaagtgtt ccagaatcat tccgagtatt gatgaaagaa ttacaaagtt
     4381 taggtttaga tgtaaaagtt atggatgagc aagataatga aatcgaaatg acagacgttg
     4441 atgacgatga tgttgtagaa cgcaaagtag atttacaaca aaatgatgct cctgaaacac
     4501 aaaaagaagt tactgattaa tacgcaattt acaaaacagg caaaaagata ctaagctgaa
     4561 ttttattgat gattcagttt agtactttaa gccattttaa ataaatgcaa atcaatcaaa
     4621 tagcacagct aatctaaatt gaaggaggta ggctccttga ttgatgtaaa taatttccat
     4681 tatatgaaaa taggattggc ttcacctgaa aaaatccgtt cttggtcttt tggtgaagtt
     4741 aaaaaacctg aaacaatcaa ctaccgtaca ttaaaacctg aaaaagatgg tctattctgt
     4801 gaaagaattt tcggacctac aaaagactgg gaatgtagtt gtggtaaata caaacgtgtt
     4861 cgctacaaag gcatggtctg tgacagatgt ggagttgaag taactaaatc taaagtacgt
     4921 cgtgaaagaa tgggtcacat tgaacttgct gctccagttt ctcacatttg gtatttcaaa
     4981 ggtataccaa gtcgtatggg attattactt gacatgtcac caagagcatt agaagaagtt
     5041 atttactttg cttcttatgt tgttgtagat ccaggtccaa ctggtttaga aaagaaaact
     5101 ttattatctg aagctgaatt cagagattat tatgataaat acccaggtca attcgttgca
     5161 aaaatgggtg cagaaggtat taaagattta cttgaagaga ttgatcttga cgaagaactt
     5221 aaattgttac gcgatgagtt ggaatcagct actggtcaaa gacttactcg tgcaattaaa
     5281 cgtttagaag ttgttgaatc attccgtaat tcaggtaaca aaccttcatg gatgatttta
     5341 gatgtacttc caatcatccc accagaaatt cgtccaatgg ttcaattaga tggtggacga
     5401 tttgcaacaa gtgacttaaa cgacttatac cgtcgtgtaa ttaatcgaaa taatcgtttg
     5461 aaacgtttat tagatttagg tgcacctggt atcatcgttc aaaacgaaaa acgtatgtta
     5521 caagaagccg ttgacgcttt aattgataat ggtcgtcgtg gtcgtccagt tactggccca
     5581 ggtaaccgtc cattaaaatc tttatctcat atgttaaaag gtaaacaagg tcgtttccgt
     5641 caaaacttac ttggtaaacg tgttgactat tcaggacgtt cagttattgc agtaggtcca
     5701 agcttgaaaa tgtaccaatg tggtttacca aaagaaatgg cacttgaact atttaaacca
     5761 ttcgtaatga aagaattagt tcaacgtgaa attgcaacta acattaaaaa tgcgaagagt
     5821 aaaatcgaac gtatggatga tgaagtttgg gacgtattgg aagaagtaat tagagaacat
     5881 cctgtattac ttaaccgtgc accaacactt catagacttg gtattcaagc atttgaacca
     5941 actttagttg aaggtcgtgc gattcgtcta catccacttg taacaacagc ttataacgct
     6001 gactttgacg gtgaccaaat ggcggttcac gttcctttat caaaagaggc acaagctgaa
     6061 gcaagaatgt tgatgttagc agcacaaaac atcttgaacc ctaaagatgg taaacctgta
     6121 gttacaccat cacaagatat ggtacttggt aactattacc ttactttaga aagaaaagat
     6181 gcagtaaata caggcgcaat ctttaataat acaaatgaag tattaaaagc atatgcaaat
     6241 ggctttgtac atttacacac tagaattggt gtacatgcaa gttcgttcaa taatccaaca
     6301 tttactgaag aacaaaacaa aaagattctt gctacgtcag taggtaaaat tatattcaat
     6361 gaaatcattc cagattcatt tgcttatatt aatgaaccta cgcaagaaaa cttagaaaga
     6421 aagacaccaa acagatattt catcgatcct acaactttag gtgaaggtgg attaaaagaa
     6481 tactttgaaa atgaagaatt aattgaacct ttcaacaaaa aattcttagg taatattatt
     6541 gcagaagtat tcaacagatt tagcatcact gatacatcaa tgatgttaga ccgtatgaaa
     6601 gacttaggat tcaaattctc atctaaagct ggtattacag taggtgttgc tgatatcgta
     6661 gtattacctg ataagcaaca aatacttgat gagcatgaaa aattagtcga cagaattaca
     6721 aaacaattca accgtggttt aatcactgaa gaagaaagat ataatgcagt tgttgaaatt
     6781 tggacagatg caaaagatca aattcaaggt gaattgatgc aatcacttga taaaactaac
     6841 ccaatcttca tgatgagtga ttcaggtgcc cgtggtaacg catctaactt tacacagtta
     6901 gcaggtatgc gtggattgat ggccgcacca tctggtaaga ttatcgaatt accaatcaca
     6961 tcttcattcc gtgaaggttt aacagtactt gaatacttca tctcaactca cggtgcacgt
     7021 aaaggtcttg ccgatacagc acttaaaaca gctgactcag gatatcttac tcgtcgtctt
     7081 gttgacgtgg cacaagatgt tattgttcgt gaagaagact gtggtactga tagaggttta
     7141 ttagtttctg atattaaaga aggtacagaa atgattgaac catttatcga acgtattgaa
     7201 ggtcgttatt ctaaagaaac aattcatcat cctgaaactg atgaaataat cattcgtcct
     7261 gatgaattaa ttacacctga aattgctaag aaaattacag atgctggtat tgaacaaatg
     7321 tatattcgct cagcatttac ttgtaacgca cgacatggtg tttgtgaaaa atgttacggt
     7381 aaaaaccttg ctactggtga aaaagttgaa gttggtgaag cagttggtac aattgcagcc
     7441 caatctatcg gtgaaccagg tacacagctt acaatgcgta cattccatac aggtggggta
     7501 gcaggtagcg atatcacaca aggtcttcct cgtattcaag agattttcga agcacgtaac
     7561 cctaaaggtc aagcggtaat tacggaaatc gaaggtgtcg tagaagatat taaattagca
     7621 aaagatagac aacaagaaat tgttgttaaa ggtgctaatg aaacaagatc ataccttgct
     7681 tcaggtactt caagaattat tgtagaaatc ggtcaaccag ttcaacgtgg tgaagtatta
     7741 actgaaggtt ctattgaacc taagaattac ttatctgttg ctggattaaa cgcgactgaa
     7801 agctacttat taaaagaagt acaaaaagtt taccgtatgc aaggtgtaga aatcgacgat
     7861 aaacacgttg aggttatggt tcgacaaatg ttacgtaaag ttagaattat cgaagcaggt
     7921 gatacgaagt tattaccagg ttcattagtt gatattcata actttacaga tgcaaataga
     7981 gaagcattta aacaccgtaa gcgtcctgca acagctaaac cagtattact tggtattact
     8041 aaagcatcac ttgaaacaga aagtttctta tctgcagcat cattccaaga aacaacaaga
     8101 gttcttacag atgcagcaat taaaggtaag cgtgatgact tattaggtct taaagaaaac
     8161 gtaattattg gtaagttaat tccagctggt actggtatga gacgttatag cgacgtaaaa
     8221 tacgaaaaaa cagctaaacc agttgcagaa gttgaatctc aaactgaagt aacggaataa
     8281 caagtatata acagaggcta atgctttagc ctcttgttat ttttatgtaa attatttgat
     8341 ttaatgttga cgaattctct tgttcaatgt taatatatta aaggttgatg caagcagaac
     8401 tttggaggat aaattattgt ctaaggaaaa agttgcacgc tttaacaaac aacattttgt
     8461 agttggtctt aaagaaacgc ttaaagcgtt aaagaaagat caagttacat ctttgattat
     8521 tgctgaagac gttgaagtat atttaatgac tcgcgtgtta agccaaatca atcagaaaaa
     8581 tatacctgta tcttttttca aaagcaaaca tgctttgggt aaacatgtag gtattaacgt
     8641 caatgcgaca atagtagcat tgattaaatg agaattagta agtgttttac ttactaaatt
     8701 ttatttaacc taaaaatgaa ccacctggat gtgtgggatt aaaaagtgaa gagaggagga
     8761 catatcacat gccaactatt aaccaattag tacgtaaacc aagacaaagc aaaatcaaaa
     8821 aatcagattc tccagcttta aataaaggtt tcaacagtaa aaagaaaaaa tttactgact
     8881 taaactcacc acaaaaacgt ggtgtatgta ctcgtgtagg tacaatgaca cctaaaaaac
     8941 ctaactcagc gttacgtaaa tatgcacgtg tgcgtttatc aaacaacatc gaaattaacg
     9001 catacatccc tggtatcgga cataacttac aagaacacag tgttgtactt gtacgtggtg
     9061 gacgtgtaaa agacttacca ggtgtgcgtt accatattgt acgtggagca cttgatactt
     9121 caggtgttga cggacgtaga caaggtcgtt cattatacgg aactaagaaa cctaaaaact
     9181 aagaatttag tttttaatta aatcttaaac ttaaa
//
LOCUS       DAKTIP010000102         9212 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAKTIP010000102 DAKTIP010000000
VERSION     DAKTIP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.102"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            352..1806
                     /locus_tag="O0305_001734"
     CDS             352..1806
                     /locus_tag="O0305_001734"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCY0274878.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            2229..2876
                     /gene="cysE"
                     /locus_tag="O0305_001735"
     CDS             2229..2876
                     /gene="cysE"
                     /locus_tag="O0305_001735"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCY0274879.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            2860..4260
                     /locus_tag="O0305_001736"
     CDS             2860..4260
                     /locus_tag="O0305_001736"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCY0274880.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            4253..4657
                     /locus_tag="O0305_001737"
     CDS             4253..4657
                     /locus_tag="O0305_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCY0274881.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            4665..5411
                     /gene="rlmB"
                     /locus_tag="O0305_001738"
     CDS             4665..5411
                     /gene="rlmB"
                     /locus_tag="O0305_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCY0274882.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLLTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            5411..5935
                     /locus_tag="O0305_001739"
     CDS             5411..5935
                     /locus_tag="O0305_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCY0274883.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARMQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            6016..6585
                     /locus_tag="O0305_001740"
     CDS             6016..6585
                     /locus_tag="O0305_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCY0274884.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            6700..6843
                     /gene="rpmG"
                     /locus_tag="O0305_001741"
     CDS             6700..6843
                     /gene="rpmG"
                     /locus_tag="O0305_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0274885.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            6899..7081
                     /gene="secE"
                     /locus_tag="O0305_001742"
     CDS             6899..7081
                     /gene="secE"
                     /locus_tag="O0305_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCY0274886.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFR"
     gene            7094..7642
                     /gene="nusG"
                     /locus_tag="O0305_001743"
     CDS             7094..7642
                     /gene="nusG"
                     /locus_tag="O0305_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCY0274887.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            7823..8245
                     /gene="rplK"
                     /locus_tag="O0305_001744"
     CDS             7823..8245
                     /gene="rplK"
                     /locus_tag="O0305_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCY0274888.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            8453..9145
                     /gene="rplA"
                     /locus_tag="O0305_001745"
     CDS             8453..9145
                     /gene="rplA"
                     /locus_tag="O0305_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCY0274889.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
BASE COUNT         3459 a         1249 c         1805 g         2699 t
ORIGIN      
        1 aattagaatc aattttaacg tatttgaata gttaatttga agtttaattt cacagtacat
       61 aatattcatt tttaagtcgt tgaaatgttt atgtagtgca gtcttgattc ttagggatag
      121 cttttttaaa gtgttgaaaa aacaacagct ttctatttaa aatttaagca tattacccag
      181 ggaattatgt tatgattaaa caatgaaatt attttaaggt catgttaaaa agcattgttt
      241 cgttttaaca ataagacgag ttagacattt gttatgacgg tcgaagatag caaatgaaca
      301 atttatatga actaacttga aaatctgata tttaatttag gagtgaaaaa aatgagcgat
      361 cgtataagag taagatatgc accaagtcca actgggtatc ttcatattgg taatgcaaga
      421 acagcattat tcaattactt gtatgctaaa cattacaacg gagattttgt gattcgaatt
      481 gaagatactg ataaaaaacg taatttagaa gatggagaaa catcacaatt tgataatctt
      541 aaatggttag gattagattg ggatgagtct gtagataaag acaatggcta cggaccatat
      601 cgtcaatctg aacgtcaaca tatctaccaa ccattaatag atcagttact agcagaagat
      661 aaagcatata aatgctatat gacagaagaa gaattagaag ctgaacgtga agcgcaaatc
      721 gctcgtggtg aaatgcctcg ctatggtggt caacatgcgc atttgactga agaacaacgt
      781 caacaatttg aagcagaagg acgccaacca tcaattcgtt tccgagtacc tcaaaaccaa
      841 acgtattcat ttgatgatat ggtaaaagga aatatttcat ttgattcaaa tggtattggt
      901 gactgggtta tcgtaaaaaa agatggcatt ccaacgtaca attttgcagt agctatagat
      961 gatcattaca tgcaaatttc agatgtaatt cgtggtgatg atcatatttc aaacacgcct
     1021 aaacaaatta tgatttatga agcatttggc tgggagccac ctcgttttgg tcatatgtca
     1081 ttaattgtta atgaagaacg taaaaagtta agtaaacgtg atgggcaaat tttacaattt
     1141 attgagcaat atcgtgactt aggttattta cctgaagcgt tatttaattt tattgcgtta
     1201 ttaggttggt ctcctgaagg tgaagaagaa atcttttcta aagaagaatt tatcaaaatc
     1261 tttgatgaaa agcgtttgtc aaaatcacca gcatttttcg ataagcaaaa attagcatgg
     1321 gttaataacc aatatatgaa acaaaaagat actgaaacag tattccaatt agcattacct
     1381 catttaatta aagcaaattt gattcctgag gtgccgtcag aagaggattt atcttgggga
     1441 cgcaaattaa ttgcgcttta tcaaaaagaa atgagttatg ccggtgaaat tgtaccttta
     1501 tcagaaatgt tctttaaaga aatgccagct cttggtgaag aagaacaaca agtgattaat
     1561 ggagagcaag taccagagtt aatgacgcac ttattcagta aattagaagc acttgaacca
     1621 tttgaagcgg ctgaaattaa aaagacaatt aaagaagttc aaaaagaaac aggaataaaa
     1681 ggcaagcaat tatttatgcc tattcgtgtt gctgtaacag gccaaatgca tggtcctgaa
     1741 ttaccaaata caattgaagt acttggtaaa gaaaaagtgc taaaccgttt aaaacaatat
     1801 aagtaatgaa atagacaaga tcaaggtagt atatacttga attttataac gtaaccacat
     1861 atgataaagt tgtattaaca aattacaaca atatgaataa agtattttat ataaaacgaa
     1921 ggattagtaa ttaaatttat acgatgcaga gagtgtacgg ttgctgtgag tacaacgtag
     1981 aaattaatga atgcaccttc gtaaaatgaa ttaaatatat aatgagagtg atgagcatta
     2041 agttgactta gtttccttga taatttggaa gcgcccgcaa tattattaat gttattcgct
     2101 aaattcagag tggaaccgtg cggaagcgcc tctaacaata caacttgtat gttagtggtg
     2161 cttttttgat atttaatttc gcaggtactt caattatgta tttacgtagt tcaatcattg
     2221 aggaggaaat gatcttgtta aaaagaatga gagacgatat aaaaatggta tttgagcagg
     2281 atccagcggc acgttcaaca ttagaagtca ttacaacgta tgcaggttta catgcagttt
     2341 ggagtcattt gattgcacat aagttataca accaaaaaaa atatgttgca gcacgcgcga
     2401 tatctcaaat ttcaagattt ttcacaggta tagaaatcca tccaggtgct aaaattggaa
     2461 agcgtctatt tatagatcat ggtatgggcg ttgtaatagg agaaacatgt acaattggtg
     2521 ataatgtgac aatctatcaa ggcgtgacac ttggtgggac agggaaagaa agagggaaaa
     2581 gacacccaga tataggagac aatgttttaa tagcagccgg tgcgaaagtt ttaggaaata
     2641 ttaaaataaa ttcaaatgta aatattggtg caaattcagt tgttttacaa tcagttccaa
     2701 gttattcaac ggttgttggt ataccaggac atattgttaa gcaagatggt gttcgagttg
     2761 gaaaaacatt tgatcatcgc catctacctg atccaattta tgaacaaatt aagcatttag
     2821 aacgacaact tgaaaagact aggaatggag agattcaaga tgattacatt atataatacg
     2881 cttacacgtc aaaaagaagt gttcaagcct atagaaccag ggaaagtaaa aatgtatgta
     2941 tgtggtccta ctgtatataa ctacattcat attggtaacg caagaccagc aattaattat
     3001 gacgtagtga gacgttactt tgaataccaa ggatataatg tagaatatgt atcaaatttt
     3061 acagacgtag atgataaatt aattaaacgt tctcaagaat taaatcagtc tgttcccgaa
     3121 attgcagaaa aatatatcgc tgcttttcat gaagatgttg gtgcgttaaa tgttagaaaa
     3181 gcgacttcaa atccaagggt aatggaccat atggatgaca ttattcaatt tattaaagat
     3241 ttggtggatc aaggttatgc atatgaaagt ggtggcgatg tttactttag aacacgtaaa
     3301 tttgaaggtt atggtaaatt aagtcatcaa tccatagatg acttaaaagt gggtgctcgt
     3361 atagatgcag gagagcataa agaagatgca cttgatttta cattgtggaa aaaagcgaag
     3421 cctggcgaga ttagttggga tagcccattt ggtgaaggta gaccaggatg gcatatagaa
     3481 tgttctgtaa tggcatttca tgagctagga cctacaattg atatacatgc gggtggttca
     3541 gatttacaat ttccacatca tgaaaatgaa atagcacaat cagaagcaca taatcatgcg
     3601 ccatttgcta attattggat gcataatggt ttcattaata ttgataatga aaaaatgagt
     3661 aaatcactag gcaactttat tttagttcac gatattatta aagaagttga tccagatgta
     3721 ctaagattct ttatgattag cgtacattat agaagcccaa ttaactataa tctagaattg
     3781 gtagaatcag cacgtagtgg actagagcgt attcgcaata gttatcaatt aattgaagag
     3841 cgcgcacaaa ttgctactaa tattgaaaat caacagacat atattgatca aattgatgcg
     3901 attttaaatc gttttgaaac agttatgaat gatgatttta atacagctaa tgcaattaca
     3961 gcttggtatg atttagcaaa acttgcgaat aaatatgtac tagagaacac aacatcaaca
     4021 gaagtaattg ataaatttaa agcagtttat caaattttca gcgatgtttt aggtgtaccg
     4081 ttaaaatcta aaaatgcaga tgaattattg gatgaagatg ttgaaaaatt aatcgaagag
     4141 cgtaatgaag caaggaaaaa caaagatttt gcacgagcag atgaaattcg agacatgctg
     4201 aaatcacaaa acattatatt agaagacaca cctcaagggg ttagatttaa acgtggataa
     4261 tcaacaagat aatcacatta aattattgaa tccattgacc ttagcatata tgggagacgc
     4321 agtcttagat caatatgtac gtacctatat cgttttaaag cttaaaagta agcctaataa
     4381 actacatcaa atgtctaaaa aatatgtatc tgccaaaagt caggcgcaaa cgttagaata
     4441 tttaatggag caagaatggt ttacagacga agaaatggat attttgaagc gagggcgtaa
     4501 cgcgaaaagt catactaaag ctaaaaacac tgatgttcaa acatatcgta aaagttcagc
     4561 gatagaagca gtgataggtt ttctttattt agaaaaaaga gaagaacgat tagaggcatt
     4621 attaaataaa ataataacaa tagtaaacga aaggtagtga cgatgtggaa gatacggtta
     4681 ttgttggtag gcatgctgtt agagaagcga ttattactgg gcatccgata aataagatat
     4741 tgattcaaga aggtattaaa aagcaacaaa ttaatgaaat tttaaaaaat gcaaaagatc
     4801 aaaaaatcat tgttcaaact gtaccaaaat ctaaattaga ttttttagca aatgcaccac
     4861 atcagggtgt tgcagcgctt attgcaccat atgaatatgc tgacttcgat caatttttaa
     4921 aacagcaaaa agaaaaagaa ggtttattga cagtacttat attagacggc ttagaagacc
     4981 cacataactt gggatcaatt ttaagaacag ccgatgcaac gggagttgat ggtgttatta
     5041 ttcctaaacg tcgttcagtt acactaacgc aaacagttgc aaaagcctca acaggtgcaa
     5101 ttgaacatgt accagttatt cgagtgacaa atttagctaa aactatcgat gaactaaaag
     5161 ataatggctt ttgggtagct ggcactgaag ctaataatgc aacagattat agaaatctag
     5221 aagcggacat gtcattggct attgtaattg gtagcgaagg acagggtatg agtcgcctag
     5281 taagtgataa atgcgatttt tatattaaga ttccaatggt tggacatgta aacagtttga
     5341 atgcttcggt tgcagcaagt ttaatgatgt acgaagtatt tcgaaaaaga catgatgttg
     5401 gagaaatata atgaaagaac gttacttaat cattgatgga tacaatatga taggacaatc
     5461 accaacgcta agcgccattg caaaagagaa tttagaagaa gctagaatgc aattaataga
     5521 tgcaattgca aattataatg cagttatttc agatgaaatt atttgtgttt tcgatgctta
     5581 tgaccaatcg ggtgttgaaa gagaatacat gtatcatggc gttaaaacga tttttaccaa
     5641 ggaaaaagaa acagctgata gtttcataga acgttatgtt tatgaacttt atgacaagca
     5701 tactaagcat attacagttg taacaagtga tatgagtgag caacatgcta tctttggatc
     5761 aggtgcatat agaatatcat ctcgcgaaat gtggagagat ttaaaagaaa atgaaattga
     5821 tgtgagtaaa tcattagatg atataagtga aaacaagcca agaactcgaa ttccgttatc
     5881 ttctgaaatc cttgcagaat ttgaaaaaat acgaagagga catcataaga aatgacattt
     5941 ccgtcatctt gaaattttga atgtaacaat attaaactaa ccttaaattt tagatagaag
     6001 gggttagttt aatatttgaa atacgatttg acaactcaag acagtacaat caaacgtaac
     6061 aatgcaataa atgataaaga cttcgaaaag ttagtaatgg atctacaacc attaattatt
     6121 cgacgcatca aaacatttgg atttaatcat tatgatttag aagacttata tcaagaaata
     6181 cttatacgga tgtataggtc ggtccaaaca tttgatttta gtggagagca gcctttcaca
     6241 aattatgttc aatgtttaat tacgtctgta aagtatgatt atttgagaaa atatttagct
     6301 acaaataaaa gaatggataa tttgattaat gaatatagag ttacgtatcc atgtgcaata
     6361 aagcgttttg atgttgaaaa caattatttg aataaattag caattaaaga gttgatttgt
     6421 cagtttaagt atttgagtgc atttgaaaaa gatgtcatgt atttaatgtg tgaacaatat
     6481 aagccgagag aaattgctca attgatgcat gtaaaagaga aagtgattta taatgccata
     6541 caacgatgta aaaataaaat aaaacgttat ttcaaaatga tttgaaaagc gccttaggac
     6601 gtgaattgaa ttataacgtg ttacttactg atggtttgac atttgttata aattttatgt
     6661 atagtatact ggtattataa tgaataaagg tgaattattg tgagaaaaat acctttaaat
     6721 tgtgaagctt gtggcaatag aaattataat gttcctaagc aagaaggctc ggcaacaaga
     6781 ttaaccttaa agaaatattg tccaaaatgt aacgcgcaca caattcataa agaatcgaaa
     6841 taaatacatt cgaaataata ctttgataat atgttcaaag gatttggagg ttgagcagat
     6901 ggctaaaaaa gaaagtttct ttaaaggcgt taagtctgaa atggaaaaaa caagttggcc
     6961 gacgaaagaa gagctattta aatatactgt aattgtagtt tctactgtta tattcttctt
     7021 agtctttttc tatgccttag atttaggaat tacagcattg aaaaatttat tatttcgtta
     7081 gaggagtgaa gacatgtctg aagaagttgg cgcaaagcgt tggtatgcag tgcatacata
     7141 ttctggatat gaaaataaag ttaaaaagaa tttagaaaaa agagtagaat ctatgaatat
     7201 gactgaacaa atctttagag tagtcatacc ggaagaagaa gaaactcaag taaaagatgg
     7261 caaagctaaa acgactgtta aaaaaacatt ccctggatat gttttagtgg aattaatcat
     7321 gacagatgaa tcatggtatg tggtaagaaa tacaccaggc gttactggtt ttgtaggttc
     7381 tgcaggtgca gggtctaagc caaatccatt gttaccagaa gaagttcgct tcatcttaaa
     7441 acaaatgggt cttaaagaaa agactatcga tgttgaactc gaagttggcg agcaagttcg
     7501 tattaaatca ggtccatttg cgaatcaagt tggtgaagtt caagaaattg aaacagataa
     7561 gtttaagcta acagtattag tagatatgtt tggccgagaa acaccagtag aagttgaatt
     7621 cgatcaaata gaaaagcttt aattaacaat taaagttatt aaactaacca aaagataaaa
     7681 aagagtattg attttttaat tagaaaagtg ttaaaattat gtggtcgcgc ttttagagcg
     7741 cccatttcgt cacgaaatgt taagagtggg agggcaaaac tgagccctgt gaccacatca
     7801 cgatatcaag gaggtgcaca tcgtggctaa aaaagtagat aaagttgtta aattacaaat
     7861 tcctgcaggt aaagcgaatc cagcaccacc agttggtcca gcattaggtc aagcaggtgt
     7921 gaacatcatg ggattctgta aagagttcaa tgcacgtact caagatcaag caggtttaat
     7981 tattccggta gaaatcagtg tttatgaaga tcgttcattt acatttatta caaaaactcc
     8041 accggctcca gtattactta aaaaagcagc tggtattgaa aaaggttcag gcgaaccaaa
     8101 caaaactaaa gttgctacag taactaaaga tcaagtacgc gaaattgcta acagcaaaat
     8161 gcaagactta aacgctgctg acgaagaagc agctatgcgt attatcgaag gtactgcacg
     8221 tagtatgggt atcgttgtag aataatttta cgaatattaa atttgattac atgatttaaa
     8281 cgatgaagca gataacagag ataataatga tgaattataa atataatctg aatgactaga
     8341 ttaatgattg atttattcat aagattaatt cttctgttgt ctgctcttaa cttgcatata
     8401 gcaagtaatg tgggaggaaa ttccgctaaa accactaaag gaggaactat aaatggctaa
     8461 aaaaggtaaa aagtatcaag aagcagctag taaagttgac cgtactcagc actacagtgt
     8521 tgaagaagca attaaattag ctaaagaaac aagcattgct aactttgacg cttctgttga
     8581 agttgcattc cgtttaggaa ttgatacacg taaaaatgac caacaaatcc gtggtgcagt
     8641 tgtattacca aacggaactg gtaaatcaca aagtgtatta gtattcgcta aaggtgacaa
     8701 aattgctgaa gctgaagcag caggtgctga ctatgtaggt gaagcagaat acgttcaaaa
     8761 aatccaacaa ggttggttcg acttcgatgt agtagttgct acaccagaca tgatgggtga
     8821 agttggtaaa ttaggtcgtg tattaggacc aaaaggttta atgccaaacc ctaaaactgg
     8881 aactgtaaca atggatgtta aaaaagctgt tgaagaaatc aaagctggta aagtagaata
     8941 tcgtgctgaa aaagctggta tcgtacatgc atcaattggt aaagtttcat ttactgatga
     9001 acaattaatt gaaaacttca atactttaca agatgtatta gctaaagcta aaccatcatc
     9061 tgctaaaggt acatacttca aatctgttgc tgtaactaca acaatgggtc ctggagttaa
     9121 aattgatact gcaagtttca aataataaat gatataaaca attacaggct gaaagaaata
     9181 tctttcagtc tgtaaaaata tattgacaat aa
//
LOCUS       DAKTIP010000103         9194 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAKTIP010000103 DAKTIP010000000
VERSION     DAKTIP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.103"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            42..566
                     /locus_tag="O0305_001746"
     CDS             42..566
                     /locus_tag="O0305_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCY0274890.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      553..650
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            725..1816
                     /gene="gcvT"
                     /locus_tag="O0305_001747"
     CDS             725..1816
                     /gene="gcvT"
                     /locus_tag="O0305_001747"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCY0274891.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLIN
                     QLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEY
                     NVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQ
                     KENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRD
                     EFEMGRELLVQVRKRQLKAKIVKKNQIDK"
     gene            1836..3182
                     /gene="gcvPA"
                     /locus_tag="O0305_001748"
     CDS             1836..3182
                     /gene="gcvPA"
                     /locus_tag="O0305_001748"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCY0274892.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            3175..4647
                     /gene="gcvPB"
                     /locus_tag="O0305_001749"
     CDS             3175..4647
                     /gene="gcvPB"
                     /locus_tag="O0305_001749"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCY0274893.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSKHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
     gene            complement(5012..5398)
                     /locus_tag="O0305_001750"
     CDS             complement(5012..5398)
                     /locus_tag="O0305_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCY0274894.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            5556..6386
                     /locus_tag="O0305_001751"
     CDS             5556..6386
                     /locus_tag="O0305_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCY0274895.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(6450..6668)
                     /locus_tag="O0305_001752"
     CDS             complement(6450..6668)
                     /locus_tag="O0305_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274896.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLISFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(6682..7263)
                     /locus_tag="O0305_001753"
     CDS             complement(6682..7263)
                     /locus_tag="O0305_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274897.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            7368..8429
                     /locus_tag="O0305_001754"
     CDS             7368..8429
                     /locus_tag="O0305_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCY0274898.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            8455..9012
                     /gene="efp"
                     /locus_tag="O0305_001755"
     CDS             8455..9012
                     /gene="efp"
                     /locus_tag="O0305_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCY0274899.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
BASE COUNT         3273 a         1242 c         1693 g         2986 t
ORIGIN      
        1 taactataag attgttttgt tattgaaaga agggaaaacg catgaatcat gataaatcac
       61 caataatttt aattggtttc atgggtaccg gtaaatctac gattggtaaa tacgttgcag
      121 atgagcaaaa tttatcattt attgatatag attcatatat cgaagagaag tataagttaa
      181 caataccaga aatattttgt aaacatggtg aacaatattt caggaattta gagttcacat
      241 gtttgcaaga atgtattaac actgcagata taattgctac tggtggtggt attattgaga
      301 gtgaagaggc atttaatttt ttgaaaaatc aaaaaaacat tatttggtta gattgtaata
      361 ttgatattat atatagtcga atcaatgatg acccacatcg acctaatgca aataataaga
      421 caatcaagca gttaaatgac ttgtattgct cgcggaattt aagatataat gaaatcgcat
      481 tcaagaaatt tgatagtcat ttgctatcaa tttcagaaat atattatgaa ttgctaaatt
      541 taataaaagc gagtgatcag tattagagag aatagagcgt taagactcta tcgccgaagg
      601 tgcaagtaat ttattacgaa actctcaggc aaaaggataa tactgtaacg cgttcctgaa
      661 ttggtgattt ataaacaggg tagcgattgc tatcctgttt ttataatttt aagggggtat
      721 ttcaatgtca agtgatttaa aacaaacacc tttatatcaa aattatgttg atagaggtgc
      781 aaaaattgtg gaattcggag gatgggcgat gcctgttcaa ttttcaagta ttaaagagga
      841 gcataatgct gttcgatacg aaattggcct gtttgatgtt agtcatatgg gtgaaattga
      901 agtaacaggt aaagatgcta gtcagtttgt gcaatattta ttatcaaatg atactgataa
      961 tttaactact tcaaaagcat tatatactgc tttatgtaat gaagaaggcg gtattattga
     1021 tgatttagta atatataaat tagctgacga caattattta ttagttgtta atgctgctaa
     1081 tactgaaaaa gattttaatt ggattttaaa acacaaagag aaatttgatg ttgaagtaca
     1141 aaatgtatca aaccaatatg gtcaattagc aatacaagga ccaaaagcta gagatttaat
     1201 taatcaatta gttgatgaag atgtaactga aatgaaaatg tttgaattta aacagggtgt
     1261 caaattattt ggagcaaacg tcattttatc tcagtcaggt tacacaggtg aagatgggtt
     1321 tgaaatttat tgtaatatcg atgatactga aaaaatttgg gatggtttat tagagtataa
     1381 tgttatgcca tgtgggttag gcgctcgtga tacattaaga ttagaggctg gattgccatt
     1441 acatggacaa gatttaactg aatcaattac accatatgaa ggtggtatcg cttttgcaag
     1501 taaaccatta attgatgctg attttattgg taaatctgta ttaaaagatc aaaaagaaaa
     1561 tggtgcacca agaagaacag tgggattaga attacttgaa aaaggaattg caagaactgg
     1621 ttatgaagtt atggatttag atggaaatat tattggagaa gtaacttcag gaacacagtc
     1681 tccatcatca ggaaaatcaa ttgcacttgc aatgataaaa agagatgagt ttgaaatggg
     1741 tagagagttg cttgttcaag ttcgtaagcg tcaattaaaa gcgaaaattg ttaagaaaaa
     1801 tcaaattgat aaataattaa aaaggggtgt gcattgtgag tcatcgttat atacctttaa
     1861 ctgaaaaaga caagcaagaa atgttacaaa caattggtgc aaaatctata ggagaattat
     1921 tcggtgatgt accaagtgac attttattaa atagagattt aaatattgct gaaggcgaag
     1981 cggaaacaac gttacttaga agattaaatc gcattgcaag caagaatatc actaaagaaa
     2041 cgcatacatc atttttgggt gctggtgttt atgatcatta cgcgccatca gttgtggatg
     2101 ctatgatttc aagatcagaa ttttacacag catatacgcc atatcaaccg gaaatttctc
     2161 aaggtgaatt acaagccatt tttgagttcc aaactttaat ttgtgaatta acagatatgg
     2221 atgtagcaaa ttcttctatg tatgatggga tgactagctt tgctgaagca tgtatcttag
     2281 catttagtca aacaaagaaa aataaaattg tagtctcaaa aggtttacat tatcaagcat
     2341 tacaagtact tcatacttat gctaagacgc gtaaggaatt cgaagtagtg gaaatcgatt
     2401 tagatggcac tgttacagat ttgaaaaaat tagaagcagc agtggatgat gaaacagcag
     2461 ctgttgcagt acaatatcca aacttttacg gttcaattga agatcttgaa aagattcata
     2521 gctttattga agataaaaaa gcattattta tcgtatatgc aaatccatta gcattgggat
     2581 tacttacacc tccaggttca tttggtgcag atattgttgt aggtgatact cagccattcg
     2641 gaattcctgc tcaattcggt gggccacatt gtggatattt tgcaacgacg aaaaagttaa
     2701 tgcgtaaagt gccaggtaga ttagtcggtc aaacacaaga tgatgaaggt aatcgtggct
     2761 ttgtattaac attacaagca cgtgaacaac atattcgacg cgataaagcg acatctaata
     2821 tttgttctaa ccaagcatta aatgctttag cgtcatcaat tgcaatgtca gcacttggta
     2881 aacaaggtat ctatgatatt gcagttcaaa atatagaaca tgctaactat gctaaacaac
     2941 aatttattaa aaaaggtttt gaagtattag atggtacatc tttcaatgag tttgtagtca
     3001 aatttgacaa accaattcaa caagttaacg aggaattagt gaagtacaat attatcggtg
     3061 gttttgattt aggtgttgtt tcagacgact ttaaaaatca catgttaata gctgtaactg
     3121 aattaagaac aaaagatgaa atcgatacat ttgtagaaaa ggctggtgag ttaaatgact
     3181 agtaaatcaa gtccattaat ttttgaaaga tctcgtgaag gcagatatgc atattcatta
     3241 ccaaaaagtg atattaaaac aaattctgtt gagtcattgt tagatgataa atttattcgt
     3301 aaaaacaaag cggagtttcc tgaagttgct gaactagact tagtacgtca ttatactgaa
     3361 ctttcaaata aaaatttcgg tgtagacaac ggattttatc cattgggttc atgtacgatg
     3421 aagtataacc ctaaaattaa tgaaaaggtt gctagaatac caggatttag tgaatcacat
     3481 ccattacaag atgaagatca agttcaaggt tcattagaaa ttatttatag tttgcaagaa
     3541 gaattaaaag aaattactgg tatggatgag gtgacattac aaccagctgc tggcgcacat
     3601 ggtgaatgga ctgcattgat gatatttaaa gcttaccatg agaataatgg tgaaggtcat
     3661 cgtgatgaag tcattgtgcc agattctgcg catggtacga atccagcctc agcttcattt
     3721 gcaggattta aatcagttac tgtaaaatca aacgaacgtg gcgaagttga tattgatgac
     3781 ttgaaacgtg ttgtaaatga aaatacagca gctattatgt taactaatcc aaacacttta
     3841 ggtattttcg aaaaaaatat tatggaaatc cgtgaaatcg tccataatgc tggtggtcta
     3901 ttatattatg atggtgcgaa tttaaacgct attatggaca aagttcgccc aggagatatg
     3961 ggatttgatg ctgttcattt aaacttgcat aaaacattta ctggtccaca tggtggtggc
     4021 ggtcctggtt caggtccagt cggtgtagta aaagaactag caagttactt accaaagcca
     4081 atggttatta aagatggcga caaatttaaa tatgataatg acattaaaaa ttctatcgga
     4141 cgtgtaaaac cattttatgg taactttggt atttacttaa gagcttatac gtatattcga
     4201 actatgggag caactggact taaagaggtt tctgaagcag cggttcttaa tgcgaattat
     4261 attaaagcac gtttatctaa acactttgaa ataccttata aacaatattg taaacacgag
     4321 tttgtgttaa gtggtgtgcg tcaaaaagaa tttggtgtac gtactttaga catggctaag
     4381 cgattattag atttcggtgt acatccacca acaatatact tcccattaaa tgttgaagaa
     4441 ggtatgatga ttgaaccgac tgagacagag tctaaagaaa cacttgatta ttttatcgat
     4501 acattaatta gtattgctga agaagctaaa aatgatcctg ataaagtgct agaagcacca
     4561 catacaactg tgattgatcg attagacgaa gctacagctg ctcgtaaacc aatattaaag
     4621 tttgaaaatc ttaaacagga aaaataaagt attaaacaca tattccgaga attattattc
     4681 taactttgta tgaagattta aggataatgg tttcaaaatc aattgaaaaa gacaatttct
     4741 atttaaacaa gaaaactaaa ccgaagtaat aactcttagg gtttggtatt attctttcat
     4801 agaaattgtc ttttcatttt ttagattgcg gtaattgaat cgtattgaaa atgagctgaa
     4861 ctttctttat tatgctgaaa ctaagtttaa tgatcagtga agctagagaa atatagtaac
     4921 agactttgtt aaaaaattaa aatctgagta ttgttattga tgatcaatta acactcagat
     4981 tttattaaat cgtatattaa atttacaaaa actatttttt agactttatt tttccagtcc
     5041 attttttata gccgcctttt aacatataga tatctgtata tccattcttt ttcaaaatac
     5101 gagcggctct atagctagca atcccattgg catcacataa gtataccggt tgatcttttc
     5161 ttaatccttg gaatcgttgc ctgaacattg tcataggaat attgcgagac ccattaatgt
     5221 gaccgtagtc atagtcaact ttctctctaa catcgatgac ttgagctttt ctaatcccat
     5281 tatggaattc attttgatct aattctttaa cagctcgctt gttaagaatt tgttgaacaa
     5341 tcatataagc aataattgct ataactaaaa ttattgcgat gtacaaacta gcactcatct
     5401 tgcatcctcc ctaattaaac gataatataa ttataagact gttaggtcaa attatcaaaa
     5461 tatttcacta aagaattgca attattattc atttagttaa attgattaat tagcgaatca
     5521 aatattatac attttgctga taggagttct tgaatatgac tgaaacttgg aattttatta
     5581 atacaggaag taaagaccca tattataata tggcaatgga tgaagcgtta ttaaattttg
     5641 tctctagagg tgaaattgat ccagttatac gtttttacac atggaatcct gcaacattat
     5701 caatagggta tttccaaagg ctacaaaaag aaattgatat tgataaggtt aaagagaaag
     5761 gttttggtct cgtaaggcgt caaactggtg gccgtggggt gctacatgat aaagagttaa
     5821 cttacagtgt gattgtgcct gaatcgcatc cgaatatgcc ttctacagtt acagaagcgt
     5881 acagagtgat ttcacaagga ttattagaag gatttaaaaa cttaggattt gatacttatt
     5941 ttgcggtacc taaaacgcct gaagagcgtc aaaaattaaa acaacctcga agttcagtgt
     6001 gttttgatgc accaagttgg tatgagctag ttgttgaagg ccgtaagatt gctgggagtg
     6061 ctcaaacgag acaaaagggc gtaatcctcc aacacggttc gattttacaa gatattgata
     6121 ttgatgaact gtttgatatg tttatttata aaaatgaaag attaaaatta aaaatgaaag
     6181 aggcattcgt tgaaaaagca gttgccatca atgatatttc agatgaacac attactatta
     6241 gtcaaatgga agaagctttc gagaaaggtt ttaagaaagg tttaaatata gaatttaagc
     6301 cacttgaatt aacagaagct caattggcag aagtggaaga attaactgaa aaatatcgtt
     6361 cagatgaatg gatgtttaga aagtagtttt tagaataaaa aatggaagca cgtagactat
     6421 tttacctaca agtgcttccg tttttttatc tattttcttc tttgatttct tttatactta
     6481 cgcattgctt tgcgatattt gcgttggtct tcagttaaga tgaagaaata ataaatcaga
     6541 taaataatac cagctattac agcaaagctg attaacattc tgacgattga aaaaataaag
     6601 gcatctagat tcattactaa tccaatcgca gcaataataa gtacaagata aaatattata
     6661 ttacgcatta aaagactcct atcatgattt tgcatttaag tttaattttg acaacgcttg
     6721 ttgtcctttt gtaagttgtt ttttatcttt attttgatag ccatttttaa tatcatcaaa
     6781 tgcatgattc aaatcagagt ttaatttatc gatattttta gacgtttttt tatcatcatc
     6841 actaagtgat aaaccatcga tagcatcttt gtatgaagtg tgagcacttt gaatatcatt
     6901 agaaatttga tttaatttcg attgaacatt cttatcgcct ttgtttttac ttacatcttt
     6961 ctcaatatcg ttataggatt tcaaagcttt agcaactttg tcgtagtatg ttgatgtaac
     7021 attcatcaat ttaattttcg tattattaga tgcagttttt ttactgtttt ctttatcctt
     7081 ttcaagccct ttaattttag atttagaatc actaatttgt tgattaagtt cttgaatatc
     7141 tagtttgagt tgatgattat cttctcttaa atctgttgtt ttttcttcta atggtgctaa
     7201 attttgtgat ccacatccag ctaacaataa tgttgcgcca acaattgaaa ccaatttttt
     7261 cattactatc tccctaaaat caatatctat tattttttat ccttattatg atattatttt
     7321 aatgtataaa gttgaaatgc acaaacgttt cactaggagg taataagatg agcagaataa
     7381 cacaagtgca tcgtatttta gaacaaaaac atttagatgc gattatcatt ctttcggatt
     7441 acaatagaag atatttatca ggttttactg gtaccagtgg agcacttatc atatctaaag
     7501 ataaacaata tttgataaca gactttagat atattgatca agcaactaag caggcgccaa
     7561 attatgaaat tattaatcgt aaatctacta ttattggtga gattaaagaa ttgctacacc
     7621 aagaaaattt tgaaaatgta ggttttgagg ggcatcatgt aagttatgat acataccttg
     7681 aattaaataa aagccgtata tcattaataa gcatttctaa tactgtagat aaaattagag
     7741 acgtcaaaga tgctgacgaa attgctttaa ttcaaaaagc agctaatatt gttgatgaaa
     7801 catatgaata tattttaact gttgtaaaag caggcatgac tgaaaaagaa ttaaaggcaa
     7861 tattagaaag caaaatgcta gaattaggag cagatggacc atcattcgat acgattgtag
     7921 catctggtca tagaggtgca ttaccacatg gtgttgcaag tgataaaatt attgaaaaag
     7981 gcgacatgat tacattagat tttggcgcgt attataacgg ctattgttca gatattacta
     8041 gaacatttgc tattggagaa ccagatccta aactgaaaga aatatatcaa atagtacttg
     8101 aatctcaaat gaaagcaatt aatgagatta gacctggcat gactggtgca gaagctgatg
     8161 ccatttcaag aaactattta gagtcaaaag ggtatggaaa agaatttgga cattcactag
     8221 gacatggtat tggtttagaa atccatgaag ggccaatgct ggctcgtacg atacaagata
     8281 aacttcaagt taacaactgt gttacagtag aacctggtgt ttatatagaa ggtttgggcg
     8341 gtataagaat agaagatgat attttaatta cagaaaatgg ttgtcaagtc tttactaaat
     8401 gcacaaaaga ccttatagtt ttaacataag cgtgtaaaat gaggaggaaa ctgaatgatt
     8461 tcggttaatg attttaaaac aggtttaaca atttctgttg ataacgctat ttggaaagtt
     8521 atagacttcc aacatgtaaa gcctggtaaa ggttcagcat tcgttcgttc aaaattacgt
     8581 aatttaagaa ctggtgcaat tcaagagaaa acgtttagag ctggtgaaaa agttgaacca
     8641 gcaatgattg aaaatcgtcg catgcaatat ttatatgctg acggagataa tcatgtattt
     8701 atggataatg aaagctttga acaaacagaa ctttcaagtg attacttaaa agaagaattg
     8761 aattacttaa aagaaggtat ggaagtacaa attcaaacat acgaaggtga aactatcggt
     8821 gttgaattac ctaaaactgt tgaattaaca gtaactgaaa cagaacctgg tattaaaggt
     8881 gatactgcaa ctggtgccac taaatcggca actgttgaaa ctggttatac attaaatgta
     8941 cctttatttg taaacgaagg tgacgtttta attatcaaca ctggtgatgg aagctacatt
     9001 tcaagaggat aatctctaat ttgttaacaa atagcttgta ttcactatac tgatttaacg
     9061 taagatattc taaataagtc tcataaagct attgcctaaa atgattatag gttatatgct
     9121 gatatgaggc tttttatttt taaaataatt tttaaaatat aaaacgaatc gtctaattgg
     9181 tagtgaaaaa acat
//
LOCUS       DAKTIP010000104         9152 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAKTIP010000104 DAKTIP010000000
VERSION     DAKTIP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.104"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..121
                     /locus_tag="O0305_001756"
     CDS             <1..121
                     /locus_tag="O0305_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCY0274900.1"
                     /translation="SVAQKINHHPRKSLGYRTPLEVFMSFIEDDKLVQLNLTI"
     gene            150..341
                     /gene="vraH"
                     /locus_tag="O0305_001757"
     CDS             150..341
                     /gene="vraH"
                     /locus_tag="O0305_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCY0274901.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            734..934
                     /locus_tag="O0305_001758"
     CDS             734..934
                     /locus_tag="O0305_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0274902.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(1061..1630)
                     /locus_tag="O0305_001759"
     CDS             complement(1061..1630)
                     /locus_tag="O0305_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCY0274903.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(1890..2285)
                     /locus_tag="O0305_001760"
     CDS             complement(1890..2285)
                     /locus_tag="O0305_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0274904.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(2353..2706)
                     /locus_tag="O0305_001761"
     CDS             complement(2353..2706)
                     /locus_tag="O0305_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCY0274905.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
     gene            complement(3159..3998)
                     /gene="noc"
                     /locus_tag="O0305_001762"
     CDS             complement(3159..3998)
                     /gene="noc"
                     /locus_tag="O0305_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCY0274906.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(4041..4760)
                     /gene="rsmG"
                     /locus_tag="O0305_001763"
     CDS             complement(4041..4760)
                     /gene="rsmG"
                     /locus_tag="O0305_001763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCY0274907.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(4760..6637)
                     /gene="mnmG"
                     /locus_tag="O0305_001764"
     CDS             complement(4760..6637)
                     /gene="mnmG"
                     /locus_tag="O0305_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCY0274908.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILELLEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(6704..8083)
                     /gene="mnmE"
                     /locus_tag="O0305_001765"
     CDS             complement(6704..8083)
                     /gene="mnmE"
                     /locus_tag="O0305_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCY0274909.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(8222..8575)
                     /gene="rnpA"
                     /locus_tag="O0305_001766"
     CDS             complement(8222..8575)
                     /gene="rnpA"
                     /locus_tag="O0305_001766"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCY0274910.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(8696..8833)
                     /gene="rpmH"
                     /locus_tag="O0305_001767"
     CDS             complement(8696..8833)
                     /gene="rpmH"
                     /locus_tag="O0305_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCY0274911.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
BASE COUNT         2843 a         1798 c         1281 g         3230 t
ORIGIN      
        1 cagtgttgct caaaaaatca atcatcatcc tagaaaatct ttaggttata gaacaccatt
       61 ggaagtattt atgagtttca tcgaagatga taaacttgtc cagcttaatt tgacaatcta
      121 aagatttata aaaaaggaga gattgaatca tgaaatttag agaattagta aaacaatcat
      181 atgaggattt gaaaaatttg actgtaactt ggtataacgt cattgcttta acaatacttg
      241 ttattgtatt aagctcattc acaacaccaa tgataggcat tccagctggt ttattaggtg
      301 gctcttatta tttaaaacgt cgtgaagaaa aaggcaagta agcacattcc atcggtatag
      361 aacattgcta tgacctttca agtcagattt taatatcaac aagtagcgtt tatcgctgct
      421 tgttttttat ggattgctta taaaatcatc atgtttaaac gatatgtcac tttgaaaact
      481 agcaaacacc ataaaagact acaatttttt tatttttata aaccccttta aatcaacgtt
      541 tctaaggttt ttaaaaatct tttttaattt attttcaaaa aacactgtac attatgccaa
      601 tatgagcgta tagttggtct tacgtaataa aagctcgtga attaaattgt agtgtatttg
      661 tttagaatat cctctttttt agttatgaat ttgttacaaa tattaagtgc aaaagcacac
      721 ggaggttttc tatatgaata acggtacagt aaaatggttt aacgcagaaa aaggttttgg
      781 tttcatcgaa caagaaaatg gcggagacgt attcgtacat ttctcaggta tcgctagcga
      841 tggctacaaa actttagaag aaggtcaaaa agttactttc gaaatcactg aaggtcaacg
      901 tggagaccaa gcagttaacg tacaaactgt ttaatcttac aacataaaac gactcattat
      961 aaatgaatcg ctttgattac caacaaggtt ctaaatcttg ttggtatttt tttatgctat
     1021 tttggataat ataaataata tgagacgata ccacgatgat ttataaaaac ttatttgcca
     1081 aatacacact aaggaaaatt atgaggctga gtataccaat tactgatata atagaaagcg
     1141 catttgtcat cgtatttcta gctttagtcg tttgtacttc actaggatct tttccattca
     1201 taaaagcaac aattcgatcg tagttgtcca ttttatgcac tttttttaaa tcttctattt
     1261 tcatagatgc ataaaaacct attcccaaaa tgattgccac cgttacaccc caagtccaat
     1321 tccaataaac aactaaaggt cccattaaaa ttgcagctaa tgtcatgaaa ataagcatca
     1381 catatgtcca taagttaaga cttcgttgcg ctttaatatc aggtacaaat ggaatggtcc
     1441 cttttactaa atcatctaaa gttacattga acaattcaca catcattaat aagttatgta
     1501 tgtctggtaa gcttttgtca ttttcccaat tagaaatact ctgcctagat acatataact
     1561 tttcagcaag atattcttgt gaataaccat ctcgttccct atacttttta atttgtttac
     1621 tcagattcaa ttacatacgc ctccagtcaa ttagtacaat cattacaatc agactatcag
     1681 ataaagttaa atcaagtcac ctttatgatt gactaaatgt tttgacatac gacaacatcg
     1741 gggttcgaag actcccttaa gacagacctg tatgattacg cgcttgtaat tttctcaatg
     1801 ccaacttgaa tttcacttat taatgatgca acttttccat gctattcaat gcttgcaaag
     1861 ttagtcctag ttaagagatt acgttcacac tatttactat tcgattttcc agctttccaa
     1921 ctatgctttt ctaatctttt ttgtgctata aattctaccg cttcaaaaat acatactgca
     1981 ctgatgaacc agcttaggaa accaacaaca atgctaatca accacatgtg cgtcatatgt
     2041 aacatcatcc atgtaactaa aatacaaact aatacccctg tcataccaaa cactgcgcca
     2101 cgacttacat gcacagcgac tttatcacca tattgcatac ctgtaataaa catagacact
     2161 aaaaatactg ccggaaacgt tgcaaatata ccgccaaatt ctttccaagg tagtgttaca
     2221 gatacaatat aacttaataa tactgcaaat ccccccacaa aaaatttcat taatgttaat
     2281 ttcatgatgt tctgcctcca ataatttatt acttaataac tgaatacaac taattatcac
     2341 gcattttcca aactataaat gtctaatact gaatattgcg agggaaatga caaaccaaca
     2401 aacaactgtg aatattgcgc ctttccgata acctttatgc ttaatgtaga gagatgtcaa
     2461 aaatacagtt aatatacaag acagaatacc gacaattgct ccagtactaa gattcatcga
     2521 catctccact aattgcgtac cacgatgatc taaagctaat gcgataatag ccgcaagaaa
     2581 gactgccggc atagtagcta taatacctcc taacttacca cctaccttat cagcaataat
     2641 tgaagctaat gcaacagcga taccaccaat gacaaaatga agaattgcac ttccaattga
     2701 aaacatcttc aacgtacctt tctataatta attcaataca aaacgtatca ttcacacaaa
     2761 gtgtaaaact taaattttct actgtaaagg caactcaagc gatgttcata aagctttcat
     2821 gatactttca taaactagca ataaagggtt caaatgaaag tatatccagc tcaaattagg
     2881 gagtgggaca gaaatgatat tttcgcaaaa tttatttcgt cgtcccaccc cagcaaggtt
     2941 gactagaatt gaaaaaagct tgttacaagc gcattttcgt tcagtcaact actgccaata
     3001 taacttcgta gagcatagaa cattgattta tgtaccagcc tgatcaacat ataaatataa
     3061 atttttatgt ttcacgtaaa acaaaaaaaa agacaaagct gttatgatct tagctttgtc
     3121 ttttatgtgt tagtcatcat gtatacgaca tcctactact aacgtttata tattcgaatt
     3181 tttatttcat aataatcttc atgatctttg tctttatgct caacatgtaa tcctgtttgt
     3241 tgaatcgctt ggatactttt gcctacctca tctcgtgctt gagtgacatc ttgtgcaaag
     3301 cgaaggtttt gtgctttgac tttttcgggc cccgtttttt ggcgtactct atcttcagtc
     3361 tgtttcacat ttagcttttg tgcaatgact tgctcaatca acgcttcttg ttcgctatca
     3421 gacaatgata ataccgctct cgcatgacgt tcagtaattt taccttctct taagcgaagt
     3481 agtactttcg gcgccaactt caataaacgc aacttatttg caataaagct ttgactttta
     3541 cctaaacttt ttgccaattc actttgcgtt gtatcaccaa tttccaataa tttcttatag
     3601 gcttccgctt cttcaacaac agacaaattt tctctttgaa tattctcaat taatgcaaca
     3661 acagccgtct cttcatcatc catatcacga ataataacgt ctgcttgagg taaatttagt
     3721 gattgtattg ctctaaatcg gcgctctcca gcaataattt caaacatatc ttcttcaatc
     3781 ggtcttacaa caataggttg tagtaaacca tgttcatgta ttgattcagc aagttcttta
     3841 attttatttg gttcaaacac ctgtcttggt tgataacggt tgggaacgat acgttcaatt
     3901 tgaatggatt caacattact attgcgatct tcttcaatat gtccaatgat gtcatctttg
     3961 tttttcaaac caaataattt tgaaaaaggt tttttcatta tccattcgct ccttttatgt
     4021 tagttgtaaa ggattatgca ttatttttca agtaaaggag tcttattagg cgtccctggt
     4081 tttcttggat actttttcgg cgtctgtctt tttttatcaa taatgaacat ctggcgctct
     4141 ccagcatctt ctggcaattc aaaggtatgt gtttctgtaa cattaccacc taacacacta
     4201 attgcaaatt ttgcttcttc taattcttct tcaccttttg aagattttaa tgcaacaaac
     4261 tgtccacctt ttttaactag cggtaaacac aattcactta acacggataa tctagctact
     4321 gctcttgcag taacaacatc ataagactcc ctgtagacac ccttaccaaa tgtttctgct
     4381 ctatcgtgta taaagctgac atcctgtaat tgtaattctg acgctaaatg gtttaaaaat
     4441 tgaatacgct tatttaatga atcaacaatc gtcactttta actgcggaaa cattattttt
     4501 aacggaatac ttggaaaacc agctccagcg cctacatcac atatacttat aggctgatta
     4561 aaatcaaaat aaaaactagg tgcaatggaa tcataaaaat gtttcaaata tacatcgtgt
     4621 tcatctgtaa tacttgtcaa attcatcttt tcattccatt caacaagtaa acgataatat
     4681 gtttgaaact gttgtttttg agtctcagtt aattgaatat tatgttcttt taattgttct
     4741 gctaaccatt ctacagtcat tagtcactca ccctttggag tttaccttgt tctaagtaaa
     4801 tcaataatat agatatgtct gctggattta cccctgatat tctagaagct tgtgcaatat
     4861 ttaaaggttt tacttctgat aatttttctc gcgcttcagt cgccaaacta tcaatcttac
     4921 tataatctaa gtcttctgga attttcttct cttccatacg cttaactttc tcaacttgtt
     4981 gtagtgattt attgatataa ccttcatatt ttgtttgtat ttctacttgt tcttcaacat
     5041 ctgcattcaa ttgatgttct tcttctaaaa gttctaaaat tatatcgtaa gtcatttcag
     5101 gtctgcgtaa taaatcgata gctaaaatac catcttttaa gcgagaacca ccatgttgtt
     5161 caataatcgc ttgcgtatgt tcgtttggtt taatacgaat atctgataaa cgcttaattt
     5221 ccgcatcaat ttgctgacgt ttttcattaa aacgtgcata tctttcttca gaaatcatac
     5281 caagttcata tcccatatcc gtcaatctca aatcagcatt atcatgacgt agtaacaaac
     5341 gatattctgc acgtgatgtt agtaaacgat aaggttcatt agtaccttta gttacaagat
     5401 catcgattaa gacaccaata tatgcatctg aacgacttaa tatcttttcg cctgtgttta
     5461 acactttacc tgcagcgtta atacctgcca tcaatccttg tcctgctgct tcttcataac
     5521 cagatgtacc attaatttga cctgcagtat ataagttttt aatcattttc gtttcaagtg
     5581 taggccataa ctgcgttggc acaatcgcat catattcaat tgcgtagccg gcacgcatca
     5641 tatctgcttt ttcaagacct ggtatcgtct ctaacatttg acgttgcaca tgttcaggaa
     5701 gacttgtaga caatccttgc acatatactt catttgtatt acgaccttca ggctctaaga
     5761 aaagttgatg tcgcggctta tcattaaatc gaacaaattt atcttcaatt gaagggcaat
     5821 aacgtggccc ggttccttta atcatccctg aatacattgc agatagatgt aaattatcat
     5881 cgataacttt gtgtgtttca gcattagtat acgttagcca acatggcaat tgatctaata
     5941 tatattctgt tgtttcaaag ctgaatgcac gacctacatc gtcacctggt tgtatttcag
     6001 tcttcgaata gtcaattgtt tttgaattta cacgcggtgg tgtacctgtt ttaaaacgaa
     6061 caatatcaaa accaagttct cttaaattgt ctgataatgt gattgatggt aattggtgat
     6121 ttggtccact tgaatacttc atattaccta aaatgatttc accacgtaaa aatgttcccg
     6181 ttgtaataat tactgcttta gataaatact ctgtaccaat atttgtacgt acacctttaa
     6241 cttcattatc ttctataata agttcgtcta ccataccttg cattatatgc aaattttctt
     6301 catcttcaat cacgcgtttc atttcttgtt gataaagtac tttatctgct tgcgctctta
     6361 gtgctcttac agcaggtcct ttacctgtat ttaacattct catttgaatg tgtgttttat
     6421 cgattgtttt tgccatttgt ccacctaaag catcaatttc acgaacaacg atacctttag
     6481 ctggtccacc tacagatggg ttacatggca taaatgcaat attatctaaa tttattgtta
     6541 gcattaatgt tttagcacca cgtcttgcag atgctaaacc tgcttctaca cctgcatgtc
     6601 ccgcacctat aacgattaca tcatattctt gaaccacaat ataaacctcc ttatttgata
     6661 tcttactagc cttcttaaga cggtattccg tctatttcaa ttactattta cctaagcaga
     6721 attgactgaa taactgatcg atgagttcat cacttgcagt ctcaccaata atttctccta
     6781 atatttccca agttctagtt aaatcaattt gtaccatatc cataggcaca ccagattctg
     6841 ctgcatcaat cgcatcttgt atcgtttgtc ttgcttgttt taataatgaa atatgtcttg
     6901 aattagaaac ataagtcata tcttgatttt gtacttctcc accaaagaac aaatctcgaa
     6961 tttgtatttc taattcatca ataccttctt gttttaacat tgaagtttga attaatggcg
     7021 tatcacctat catatcttta acttcattaa tatctatgtt ttgctctaaa tccattttat
     7081 taacaattac gattacatct tcatttttaa ccacttcata taatgtgtaa tcttcttgag
     7141 tcaatgcttc gttattgttt aatacaaata aaattaagtc tgcttggcta agagcctttc
     7201 tagagcgttc aacaccaatc ttctctacta tatcttctgt ctcacgtata ccagcagtat
     7261 caactaatct taatggcacg ccacgaacat tgacgtactc ttctaagaca tctctagtag
     7321 tacctgctac ctcagttaca atcgctttat tatcttgtat taaattattt aacatcgatg
     7381 atttacctac gtttggttta ccaacaataa ctgtagataa accttcacgc ataattttac
     7441 cctgcgcacc ggtatctaat aaacgattaa tttcctgttt gatttcttta gactgctcta
     7501 aaagaaattc agtagtcgca tcttcaacat catcgtattc aggataatca atattcactt
     7561 ccacttgagc gagtatctct aatatagatt gacgttgttt tttgattaag tcacttagac
     7621 gaccttcaat ttgattcatc gcaactttag aagctctatc tgtcttcgag cgaataaagt
     7681 ccataactgc ttcagcttga gataaatcaa tacgaccatt taaaaaggca cgttttgtaa
     7741 attcacctgg ctcagccatt ctagcgccat atgtcatagt aagttccagc actctattaa
     7801 tcgttaaaat accaccatga caattaattt ctataatatc ttcgcgtgta aatgtttttg
     7861 gcgctcttaa cacagacacc ataacttctt caatcacttc tttagactct ggatcaataa
     7921 tatgaccgta attaatcgta tgtgatggaa catcatttaa aagatgtttt cctttatata
     7981 atttgtcagc aatttcaacg gcttgcggtc cagacaatcg aacaattcca attgcccctt
     8041 cacccattgg tgttgaaata ctcgtaattg tatctaaatc catattgcta ctcgcctcct
     8101 tcaacgatgt gaatacattt taaagtaagt tattataacc ctaaggtcag tcttacgttt
     8161 gtctgaggta agacttcggg atgtgttgag tggttaatgt tttccttccc ctaccctatc
     8221 cttacttaat ctttttatta aaaactttgg caattttaag tacgtgctca agactattct
     8281 gtatttgtaa agtcgtcata tctttagctg gctgtcttgc tattacaata atatctttgg
     8341 ccaatatatg cgacttatgt actttgaaat tttcacgtat tgctctttta atcttgtttc
     8401 ttaacactgc attacctagt tttttagaaa cactaatacc taagcgaaaa tggtctattt
     8461 ctttattatt acaagtgtat acaacaaatt gtctgttggc tacagaatga ccttttttat
     8521 atattctctg aaaatctgca ttctttttaa ttcggtaagc tttttccaat aacatcactc
     8581 gcttatttat cgtttttatt tgaagctata tttaaacttc tattgagctt ataacataaa
     8641 tttctattta ttcttaattt aaacgaaaaa aaagatcact gatgagtcag tgatcttatg
     8701 cagataaaac tttacggcct ttacgacgac ggcgcgctaa aactttacgg ccattttttg
     8761 tgctcatgcg ttttctgaaa ccatgaactt tactatgttt acgtttattt ggttgataag
     8821 tacgttttac catgcaaaac acctccatct ttatacagtt atctaatata ccagattatg
     8881 cagactctga tcactttaat agataagatt ttgcgtttct tagattaaaa ttctcgtatt
     8941 agctcattga ttatctagtc ataattcaag caactactac aatataacaa aatcctattt
     9001 ataacgcaag ttcattttat actactgctc aattttttta cttttatcga ttaaagatag
     9061 aaatacacga tgcgagcaat caaatttcat aacatcacca tgagtttggt ccgaagcatg
     9121 agtgtttaca atgttcgaac accttataca gt
//
LOCUS       DAKTIP010000105         8927 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAKTIP010000105 DAKTIP010000000
VERSION     DAKTIP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.105"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1063)
                     /locus_tag="O0305_001768"
     CDS             complement(<1..1063)
                     /locus_tag="O0305_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCY0274912.1"
                     /translation="MTLKNQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKGGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQ"
     gene            complement(1238..1540)
                     /locus_tag="O0305_001769"
     CDS             complement(1238..1540)
                     /locus_tag="O0305_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274913.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            complement(1554..1874)
                     /locus_tag="O0305_001770"
     CDS             complement(1554..1874)
                     /locus_tag="O0305_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCY0274914.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELDYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            complement(2025..2309)
                     /locus_tag="O0305_001771"
     CDS             complement(2025..2309)
                     /locus_tag="O0305_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274915.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGVSKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            complement(2318..2746)
                     /locus_tag="O0305_001772"
                     /pseudo
     CDS             complement(2318..2746)
                     /locus_tag="O0305_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(2941..3165)
                     /locus_tag="O0305_001773"
     CDS             complement(2941..3165)
                     /locus_tag="O0305_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCY0274916.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVARDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(3182..3385)
                     /locus_tag="O0305_001774"
     CDS             complement(3182..3385)
                     /locus_tag="O0305_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HCY0274917.1"
                     /translation="MSKINHITIVLSFTKVDANGKQTEFKRRFANINPDASNDQIKTF
                     SKLIERLTGETYSNIELIKSLSI"
     gene            3544..4104
                     /locus_tag="O0305_001775"
     CDS             3544..4104
                     /locus_tag="O0305_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCY0274918.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVSQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            4123..6525
                     /gene="mnhA2"
                     /locus_tag="O0305_001776"
     CDS             4123..6525
                     /gene="mnhA2"
                     /locus_tag="O0305_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCY0274919.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAIQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYVYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVLALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            6512..6937
                     /gene="mnhB2"
                     /locus_tag="O0305_001777"
     CDS             6512..6937
                     /gene="mnhB2"
                     /locus_tag="O0305_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCY0274920.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            6934..7278
                     /gene="mnhC2"
                     /locus_tag="O0305_001778"
     CDS             6934..7278
                     /gene="mnhC2"
                     /locus_tag="O0305_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCY0274921.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            7268..8764
                     /gene="mnhD2"
                     /locus_tag="O0305_001779"
     CDS             7268..8764
                     /gene="mnhD2"
                     /locus_tag="O0305_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCY0274922.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            8765..>8927
                     /gene="mnhE2"
                     /locus_tag="O0305_001780"
     CDS             8765..>8927
                     /gene="mnhE2"
                     /locus_tag="O0305_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0274923.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRK"
BASE COUNT         2882 a         1388 c         1432 g         3225 t
ORIGIN      
        1 tttgttttaa agcctccaaa taatgattaa ttctattcgt atgctcatca ttaaattgtg
       61 aatgcgctaa aattttatct aaaaaatcag tatctgtaag caacccctca gattgcgcca
      121 tactttcggc agttaacact gtatctaaag tagtagcaac ttgtttacat aaccaatcaa
      181 gattatcctc aatggacttt aatgttggtc tagtagttaa attcaatttt tcagtatctt
      241 taataaataa cgcccaaggt ctatataatg gccaccgctt acgattttta tcatttccac
      301 ctttaggagg agagataaaa cgcaaattat tatgcaatat agactttaca atttcgctta
      361 tattatctgt tctactcaac actttcgcac aattaactgc atatgccttt cgcatttgta
      421 tttcataacg attccatagc ccaatgtctt cgacatcaca atgacgttta aacgcttgtt
      481 cataattctt ttcataaaaa cgacaatata aattgctttg tttagaacca atataaaaag
      541 tagcgccttt acttcttccg tctgacaaat taaaaccgtt tattgcgctt ccagctctaa
      601 aacgtgaaac acactcagct ttttcagcct ttttaattaa taaaggcact tttaaatatg
      661 ttttataatc atcaattgca atatcaattc ttgtaaaatt tgcattaact gataaacatt
      721 tgtcaaaaaa atcttgccaa gttttattca actgctgtaa atgatgttca aactctctac
      781 aacctgtacc agttaactgt aagtgaatgc ccattgattc atcatcactt gaataataca
      841 atttaatgtt tgaaaattca taaccgcgat tataaaagta aatgccacta tttcgttttt
      901 tgaacaacgt tataggcaaa ccgataacgt cctcaattac tgctgaaata ggggctatat
      961 ggaaagtgac ctgaacccag tcaacaactg cctccacagc acttttattt gtacgctcta
     1021 cccccctatt agtaaggggg gtacactggt tttttaaagt catatttatt tatgcctcct
     1081 atcaaatcgc aagcgatttg atacatgacc gttctcgctt ttttaacgcg tatttgtcgc
     1141 aggctccgcc caaatgctta acgcactccg tgcttgcatt tctgcgcctg catacgctaa
     1201 aagctacacg gtcatgttaa atcactaata ctatatgtta aacatcaata tcgccaatat
     1261 aaatacaatc ttccaaattc acttgtagca ttctgccatg tcctgcttca ctcaagctca
     1321 tacacaatag acaaaggtca gcgtaaattt taggccgacc ttttaccgta ttttctttaa
     1381 cgtaactatc attaaattta gactgtattt ctttagctaa agtcattaat gaaactttat
     1441 cgccccaatt tatacgacta gcaacatagt ttaaaatagg ttcactcaaa tttgaactat
     1501 ttgtgtagcg atataatcta tctgctgtat aataatccat ttacatcacc taattatttt
     1561 tgtttaagtg cactattaga accaactact ttaatatctt ctgcattcca gctatatgcg
     1621 atagttccaa aatcgcctga catttgaaca tacattttaa cgtctggttc aactaattcg
     1681 cattcttgta aatagtctaa ttccacttta ttaggcgttt taacagtgat ttgttcgcct
     1741 tgttcttgac tcgctaattt atatgcataa acagttttat cagtacgctc tccattttca
     1801 tatttatatt tttcatctac acctaaaaag tttaattgtc ctaaagtttt tttaatatcc
     1861 aatactggtt tcataatata tccatccttt acaaaatttt taataaaatt aaacgacttc
     1921 taaagggtcg cacgaatagg aataactccc gcaaatccgt accactgcat ttttgataat
     1981 ggagtgtcta tgcttctcct gacctatcat atttttaatc aaacctatcg gttcatcacc
     2041 tcaaaaaatt ctaagtcttc aattgaaata tgatacatct tacaaataaa tgtattagac
     2101 acaccatatt gttttaaaaa tttataatcc ttataagtaa atcgataatc attctttttt
     2161 gccatttcac aaacttctaa tatacgaaaa tatgcaaaat tatacatttt acaaattttt
     2221 cttttagaaa caccatgaaa tcttaaaatt acaaatttaa gataatttaa ttgatttgaa
     2281 ccatcaaaaa aaggtgtaaa ctcagtcata attatcaatt cctttcataa gaattaactg
     2341 ttaactaaag tttacaccac caattaaaat catgaattgt agcgccaaat aatccaaatg
     2401 tatcaataat gacacccata ataatttgac ccgcaactgt tgcaattaca gttaatgttg
     2461 cacctaattt tggcaataac aataaattgc cagttaaaaa gctaacccca agcaaaccac
     2521 cgactaccca tgtgtagtta aatgattgat tattgtaaaa gtgaatagta aatacttctg
     2581 gattgataat gatatttaaa ataattaaac aaattgttcc aactgaaaat gaaatgaatg
     2641 aagtatagaa aggcgattta gtatacagtg atagtcttga attgacagat gtttggatag
     2701 gaataagcat tccaactagg acaccaatga tatagaaaag aagcatatga ataaagtctc
     2761 actttccaaa gttaataata aaatcatata tttacattat cattatagag aaaatataca
     2821 actcatttac aataatgttg aaaaaaacag gacattatta caacaaaatc accctagaca
     2881 atcatgatga aagtctaggg tgttagtatt tatactaaat taattgcgct ttctttatat
     2941 ctatttctta tcttcaaata aaacagttgt tgttttatca ataatttgag cagaatgtac
     3001 tttggtgata gtaccactat ttgactttat aatatttaaa tctactaatg cattcatact
     3061 atcacgtgca acttgctcgg tcactacgtt gtttaattgt ggtaattgca gttttacagg
     3121 cttgtctagt gaagatttaa aacttaattc aagtgtttta gtcatgaata tgtcctcctg
     3181 attaaattga taaagatttg atgagttcga tattgctata tgtttctcca gtaagtcgct
     3241 caataagttt gctgaatgtt ttaatttggt cgtttgatgc atcagggtta atgttagcga
     3301 atcgacgctt aaattctgtt tgtttgccgt tagcgtctac tttagtaaat gataatacaa
     3361 tagtgatgtg gtttatttta ctcatatttt aaaacctcct ttcacactat atatcgaaac
     3421 aaaataataa aatggctaat tttattttct atgtttaaaa tctataaaaa aggcaataga
     3481 tatatgtaac taaaatataa tatagctatg attaagacac ttaaagcaag gggaggttga
     3541 gtaatgaata aagtagaagc gattaaattt aatgatgata ttgttaaaat gtatgaagcg
     3601 ctcaagataa aatctgaacg tgactattta ttctttaagt tagctataca tagtggattg
     3661 aaagtatcag aattattaac aattacagtc tctcaagtta agagactaat tgaaaagtgt
     3721 acgttatcag aaatgtgtaa agcacatttt cattcgttga ttaaaattag gttaccagaa
     3781 acattatcga aagaactact tcaatatata gaggacagga gtctttcgaa tgaagacgtt
     3841 ctttttcaat cactacgaac aaatcaagta ttatctagac agcaagcata tcgaataatt
     3901 caccaagcat caattgaagc tggtatagat aatgtaggac taacgacatt gcgtaagaca
     3961 tttgcatatc atgcttatca aaaaggtata cctataccag tcattcaaaa gtatttaggg
     4021 catcaatctg ctattgaaac actaaatttt atcggtttag aaaatgagtg tgaacatagt
     4081 atttatattt cattacaatt atagaaacaa aggaggctaa taatgagttt ggtttattta
     4141 ttaattgcta tacttgtgat tatggcgatg atacttctaa tgtctaaacg tagagcattg
     4201 gctaaatatg ccgggtacat agcgttggtt gcacctgtaa tttcatctat ctattttttg
     4261 attcaaatac catcagtagc taaactgcaa tatctttcta cctctattcc atggattaag
     4321 acattagata ttaatttaga tttacgttta gatggtttaa gtttaatgtt ttctcttatt
     4381 atttcactta ttggaattgc agtattcttc tatgcaactc aatatttatc ctctcgaaaa
     4441 gacaatttac caaggtttta tttttattta acgttattta tgttcagtat gattggtatt
     4501 gtattatcag acaatacgat attgatgtac attttttggg aattaacgag tgtatcatca
     4561 tttttattga tttcatattg gtataacaat ggtgacagtc aatttggtgc gattcaatca
     4621 tttatgatta cagtatttgg tggattggcg ttattagttg gttttattat gctgtatatc
     4681 atgacaggaa cgaataacat cacagagata ttaggacaag cagatcatat taagaatcat
     4741 ggattgttta tccctatgat ttttatgttt ttattaggtg catttacaaa atcagcacaa
     4801 tttccatttc atatttggct acctagagca atggctgcac ctacacctgt aagtgcttat
     4861 ttacattcag ccacgatggt aaaagctggt atctttttat tacttcgatt tacaccatta
     4921 ttaggtctta gcaatatgta cgtatatatc gttacgtttg ttggtttaat aacaatgtta
     4981 tttggttcaa ttacagcttt aaaacaatgg gatttaaaag gtatcctagc gtactctaca
     5041 atcagtcaac ttgggatgat tatggctatg gtgggtatag gtggcggata tgctcaacac
     5101 caacaagacg caatagcatc tatttatgta tttgtattat ttggtgcgct atttcatcta
     5161 atgaatcatg ccatctttaa atgtgcgctt ttcatgggag taggtatttt agatcatgaa
     5221 gcaggttcaa gggatatacg aattttaagt ggaatgcgtc aactatttcc taaaatgaat
     5281 ctagtcatga cgatagcggc tctatctatg gctggagtac catttttaaa tggattttta
     5341 agtaaagaaa tgtttttaga tgcattaaca caaactggac aattatccca atttagtttg
     5401 atttcaatga tagctatcgt gtttgttggt gttattgcga gtgtttttac attcacatat
     5461 gcactataca tggtaaaaga agtattttgg acaaaatatg attctaaggt ttttactaaa
     5521 aaaaatatcc acgaaccatg gttgtttagt ttaccatctc ttatattaat ggtgctagta
     5581 cctgtaatct tttttgtacc aaatatattt gggaagggga ttatcgttct agcattaaga
     5641 gctgtatcag gtggtaatca tcaaattgat caattggcac cacatgtttc gcaatggcat
     5701 ggatttaaca taccgcttct tttaaccatc atcattattt tattgggtag tgtactagca
     5761 atcaaagtag attggaaaaa agtgttcaca ggtaaaatta gacagatttc agtttcaaaa
     5821 agctatgaga tggtatatcg acattttgaa aagtttgcta cgaagcgatt taaacgtgtt
     5881 atgcaagatc gtttaaacca atacattatt atgaccttag gcatatttat gattatcatt
     5941 ggatatggtt atattcgaat tggacttcct aaagtacatc agttacatgt ttctgaattt
     6001 ggggcattag aaattatatt agcaatcgta actgtcacaa ttggtatttc tttaattttt
     6061 atacgtcaac gactgacaat ggtcatttta aatggagtca tcggatttgt tgtgacctta
     6121 ttctttatag caatgaaagc ccctgatcta gcattgactc agctagtagt tgaaacaata
     6181 acgacgatac tatttattgt cagtttttca agattaccaa acgtgccaag atctaacgct
     6241 aacaaaaaaa gagaaataat taaaatttct gtatcactct tgatggcact tattgttgta
     6301 tcattaattt ttattacaca acaaacagat ggtttatcat caatatcaga cttttattta
     6361 aaagctgaca aactaacagg tggtaaaaat attgtaaatg cgatacttgg tgactttaga
     6421 gcattagata cattatttga aggattagtg ttaattatta ctgggctagg tatttacaca
     6481 ttattaaatt atcaagatcg gaggggacaa gatgaaagag aatgatgtcg tgttaagaac
     6541 ggtcacgaaa cttgttgtat ttattttatt gactttcgga ttctatgtct tcttcgcagg
     6601 tcataataat cctggtggtg ggtttattgg tggtttaata tttagttcag cgtttatttt
     6661 aatgtttctg gcttttaatg ttgaagaggt tttagaaagt ttaccgattg attttagaat
     6721 tttaatgatt attggagcat tggtatcatc tattactgcg ataataccta tgttttttgg
     6781 aaaaccattt ttgtctcaat atgaaacaac ttggatactt ccaattttag gacaaattca
     6841 tgtaagtaca ataacacttt ttgaattagg tattttattc tcagttgttg gtgttattgt
     6901 cacagtgatg ttgtcgctta gcggaggtcg atcatgaatt taatattatt actagttata
     6961 ggatttttag tgtttatagg aacatatatg attttatcaa tcaatttaat tcgtattgta
     7021 atcggaattt caatatatac tcatgctggt aatctcatta ttatgagtat gggaacgtat
     7081 ggttctagta gatcagaacc actaataact ggtggaaacc aattgtttgt tgatcccttg
     7141 ttacaagcta ttgtactaac tgcaatagtt atagggtttg ggatgactgc gtttttactt
     7201 gtacttgttt atagaactta taaagtaaca aaagaagatg aaattgaagg cctaaggggg
     7261 gaagatgatg ctaagtaact tattgatttt accaatgtta ttaccattcc tttgtgcctt
     7321 aatccttgta tttttaaaaa ataatgatcg tatttctaaa tatttatact taggtacaat
     7381 gactatcacc acaattattt cattaatgct attaatttat gttcagcgtc accgtccaat
     7441 tacgctagac tttggaggat ggtcagcgcc ctttggtata cagtttttag gagattcttt
     7501 aagtttaatt atggttacaa ccgcttcgtt tgtgattact ttaattatgg catacggatt
     7561 tgggcgtggc gaacataaag caaatcgtta tcacttgcca tcgttcatat tatttttaag
     7621 tgttggcgtg ataggctctt ttctaacatc agatttattt aatttatacg tcatgtttga
     7681 aattatgtta ctagcgtcat ttgtactcat tacacttgga caatctgtag aacaattacg
     7741 tgctgcaatt atttatgttg tcttgaatat tattggttca tggctattct tattaggtat
     7801 aggtttactt tataaaacag taggtacatt aaacttttca catattgcaa tgcgtttgaa
     7861 tgacatggga gataatcgca ctgttacaat gatttcatta atcttcttag tcgcatttag
     7921 tgcgaaagca gcgctggtcc tttttatgtg gctacccaaa gcctacgctg tgttaaatac
     7981 tgagcttgca gcattatttg cagcgttaat gaccaaagta ggggcctatg cattaattcg
     8041 attcttcact ttactatttg atcaacataa tgatctcata catccattgc tagcaactat
     8101 ggctgctata actatggtca tcggcgctat aggtgtcatt gcttataaag atattaaaaa
     8161 gattgcagct taccaagtca taatctcaat aggatttatc attttaggtt taggaacaaa
     8221 cacgtttgca ggtattaatg gtgcaatatt ttatttggta aatgacattg ttgtaaaaac
     8281 attgctattt tttattattg gtagtttagt ttacattaca ggctatcgac aatatcaata
     8341 tttgaatggc ttagctaaaa aagaaccttt atttggagtt gcgtttatta taatgatttt
     8401 tgctattggc ggcgtgcctc catttagtgg ctttccgggg aaagtactta ttttccaagg
     8461 tgcattgcaa aatggcaatt atattggact agcgttaatg attattacta gtctaattgc
     8521 aatgtacagt ttatttagga tactttttta tatgtatttt ggagataaag atggggagga
     8581 agttaatttt aagaaaatcc cgctatatcg aaaaagaatt ttaagtattt tagtagttgt
     8641 ggttatcgca atcggaattg ctgcacctgt tgtgttaaat gttacaagtg atgcaactga
     8701 gttgaacacg agtgatcaat tatatcaaaa acttgtaaat ccgcatttga aaggagagga
     8761 ctaaatgaat caaatagttt taaatattat cattgcattc ttatgggtat tatttcaaga
     8821 tgaagatcat tttaaattct cgactttctt ttctggatat ctaattggtt taattgtcat
     8881 ttatatatta cacaggtttt tcagcgatga tttttatgtt agaaaaa
//
LOCUS       DAKTIP010000106         8851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAKTIP010000106 DAKTIP010000000
VERSION     DAKTIP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.106"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            88..1611
                     /locus_tag="O0305_001781"
     CDS             88..1611
                     /locus_tag="O0305_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274924.1"
                     /translation="MSKKLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFNNAQAQQSDYSKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYNDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1679..2845
                     /locus_tag="O0305_001782"
     CDS             1679..2845
                     /locus_tag="O0305_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274925.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALLITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRTLITWFYTMLWSIVGVVWIVFSIPNIFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2842..3762
                     /locus_tag="O0305_001783"
     CDS             2842..3762
                     /locus_tag="O0305_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0274926.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3776..4435
                     /locus_tag="O0305_001784"
     CDS             3776..4435
                     /locus_tag="O0305_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0274927.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4487..5437)
                     /gene="lytM"
                     /locus_tag="O0305_001785"
     CDS             complement(4487..5437)
                     /gene="lytM"
                     /locus_tag="O0305_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCY0274928.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5763..6755)
                     /locus_tag="O0305_001786"
     CDS             complement(5763..6755)
                     /locus_tag="O0305_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0274929.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWKSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6989..8365
                     /locus_tag="O0305_001787"
     CDS             6989..8365
                     /locus_tag="O0305_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0274930.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8442..8792)
                     /locus_tag="O0305_001788"
                     /pseudo
     CDS             complement(8442..8792)
                     /locus_tag="O0305_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         2978 a         1489 c         1430 g         2954 t
ORIGIN      
        1 ttaatttaaa caaaatttaa aataaaccat ttaataaaat gtattgttct gatatgatta
       61 actcataaat aattgggagg aagcaaaatg tctaaaaagt taaaaattat aattcctatt
      121 attattgtct tactattaat aggtggaatc gcatggggag tttatgcatt ttttgctaat
      181 acaccgaaaa atacatacct aaaaagtgaa caacaaacag caaaaatgta taaagattat
      241 tttaatgacc gttttgaaaa tgaagtgaag ttccaagaaa agatgaaaga taattcgttt
      301 ttatcttcat tagaattaag cgcagatgca tcagatgaaa ttgttaaagg gcttggcatt
      361 cctaaatctg ttgttaatgc atcgaaaatt aaaatgtcat atggacatga tcctaaaaaa
      421 gagaaatcaa tgattaatct tgaaccaaca atcgcagact ctgcattagg gaaattccag
      481 ttagctgcag ataaagataa gcattatttc gaatcaccat tatttaaagg gaaatatagt
      541 gttaataatt ctgatttatt atcaacttat tcaaaactta caggtgaaga tgaagaaaca
      601 gcaaaagaaa atggtattac aaaccaacaa ctaaatttaa atactctttt taataatgct
      661 caagcacaac aaagtgacta cagcaaaatt gccgaaaaat attccgaact tattgtcgac
      721 aaattagatg acgataattt tgataaaggt aaaaaagaag aaattaaggt taatggtgaa
      781 aagtacaaag ttagacctgt cacgttaaca cttagcagag ctgacactaa aaaaattaca
      841 ttagctgtat tagaagaagc taaaaaggat aaagacctta aaaaattaat ggaagaacaa
      901 ggtgctacaa aagactttga aaaagacatc aaaaaagcaa ttgacgatgt caaagaaact
      961 aaaaaggatg aatttgctaa aattcaatct aaaatttata ccgaaaaaca tacgattgta
     1021 aaacgagaaa ttactattac agacaaagaa aataataaaa ctaaaatcaa aggtactaat
     1081 actttagaag acgataagtt aaaactagat tacgcacttg atttcgatca agataaatac
     1141 acgtatgctg aagcgaaata tacaattaaa ggcgtatctt ctaaagaaaa agataataaa
     1201 tataatgata aatacgaatt tggtaaaaag acagaatatg atgaatcaaa aatcaaatta
     1261 gataaccaag aaaaagtaga tggcacaaaa cgtcaagata aaggtaaaat cactgtcgca
     1321 ttagataaat atagcgacga aaatgaattc acttttgaaa ataatataga ttctgacgta
     1381 aaaaataaca ctcagaaatc tacgttaaat atcggcatca aatatgctga agaaccaatt
     1441 aatttcattt taaaatctag cacaaaattg aaagcagata ttgattttga tgatagtggt
     1501 gcaaaagatt tcaatagtct atcttcaaaa gaccgtgaaa aacttgaaaa agaaatcgaa
     1561 aaaaatggcg gcaaaatgtt tgaatcaatt ttaaaaaagg catctaaata actaaaatat
     1621 aaaaataaga ttggaaggtc atcttgcctt tcaatcttat cattggagga gtggttaaat
     1681 gaaaacaata catttgtttc gcatctacca ctctttttta ttgaaaaagt ggtatttgat
     1741 tatctattta ctatttatat tagcggcact ccttattaca ttaacgacaa tccaacatgt
     1801 aacagaagat gacaatcatt ttaatatagg tgtcgtagat aaagatcaat caagtgaaac
     1861 gaaattaatc ttaaactcta ttggtaaagg gagtaaccta ggaaaaaacg tgagcattaa
     1921 agcatatgat gataagcaag cacatacttt gttaaaaaaa cataaacttc aaggctattt
     1981 tgtttttgat aaaggtatga ccaaggcatt ttataaacaa ggcgaactac caatttcagt
     2041 atatacatat gatcaacaat ccatgaaaag tgtcgtgcta tctcagctaa cagattctgt
     2101 ttaccaacgt cttatgcgat caatgggtgg catcttagct tttcaagact tagcaccgaa
     2161 agcatcacat tctgacagta tcaatgttat gactgatttg ctgattacag gattaaaccg
     2221 ttcaggtgca tttaacttag aaccgattca tttatacgat acgggcagtt attatgcaat
     2281 tacaggattt ttaacaacgg tattcatctt tgcactatct ttatttacag ttttgaaaat
     2341 gaatcaagat actgtattga aagcgcgatt gaaaatgttt catttttcta aagagcgttt
     2401 attaatcatt cgtacgttga ttacatggtt ttatactatg ttatggagta tcgttggtgt
     2461 agtttggatt gtgttcagta ttccgaatat ctttgaatta tataattggc caacgttagc
     2521 cattcattta agctattatg ttaccttttt aatactgtgg ttattactca tcgaactttt
     2581 aacaacaggc ttgttgaata gtattagtaa agtcatatta gctattgtca tcctggtgtt
     2641 atctggttta acaataccta cgattttttt acaacatata gcaaatggtg ttttcaatat
     2701 tcaaccgttc gcagttgtaa caaatcaatt gttagaaatt attttaaaca actacatttt
     2761 agaactgcac cctagtttct atcttagctt catcgcactt ttgataatta atttagctgt
     2821 attggtttgg aggtatcgtc aatgaagcct tatatacaac ttgttgtgtt caagcaatgg
     2881 ttacaataca tcttgctcgt aacaaccatt gtcatcgcac tcgtacttat tggtatcggt
     2941 taccgtgtag cacatgacaa cttcaaaata ccgattacca ttcaagattt agaccaaacc
     3001 actgcatcaa aatcattcgt caataaaatt aaacaatctg actatgtaac tattaaaaaa
     3061 gtcgatgaag atgaaagcta tattgaagat gatgttacta aaaaggaagc tattttaagt
     3121 atgcaaattc ctaaaggttt ctctcaaaaa ttaaaagaga accgtttaaa agaaacgata
     3181 cagttatatg gtagagatga ctttataggt ggtattgctg tagaaattgt tagtagttca
     3241 ttatatgagc agcaaattcc taacattatt tatgaacacc ttgaggatat gaaacagcat
     3301 caatccatcg atgctatcaa caagtcctat cataaacata cacctgaatc taaaatcaaa
     3361 tttgtgtcgc ttactaaaca agcacaacac tctatttcaa ttagcttaat ctttgcggtg
     3421 attttatttg ttagcgctgt tcaagtagta cttcattatc gtttaaacca acaagcagca
     3481 ttgcaacgat tatcacaata tcatttaagc cgtttcaaac tatatagtac ttatgtaatg
     3541 acacatacga ttttgttatt gttggtacta ttggcagtta gtctatattt gtctcaacca
     3601 ctcagcttaa tattttactt aaaatcactg ttacttatat tgatttatga gataggtatc
     3661 gtctttatct tattccatat tcaaacaata agtcatcgat tattcatgac atttatatat
     3721 gcacttgcta tgggtatcgt atacttgatt attttcatgt aaaggagcgt aactgatgat
     3781 agaaattaat aacctttcaa agcgttaccg taacaaacag attttcaatc atttaactat
     3841 gtcctttgat agtaatcgtt taaccgtatt acttggtgat aatggtgctg gaaaatcaac
     3901 attacttcgt atgattgctg gtattgaaaa agctaatgat ggaactatca actatttcgg
     3961 cgaaaaatgg aatcaaagac aaatacaaaa tcacatcggt tatgtgccac aagacattgc
     4021 gttatttgaa cacatgacag tggctgaaaa cattaaattt tttaaatcac tttgtaaaaa
     4081 tccaattaac gatacaacta tcaacgaata tttacagcaa ttaaactttg atgatacgtc
     4141 tgccaaagta tctacattgt ccggtgggaa taaacgtaaa attaatatat tagtaggttt
     4201 actaggtcaa cctcgaattc tcattttaga tgaaccgaca gttggtattg atttaaaatc
     4261 tagacatgac atccaccaac tacttaacat catgaaatct aaatgtttaa ttatattaac
     4321 tacccatcat ttagatgaag ttgaagcact tgcagatgat atcaagttaa ttggccaaga
     4381 tcctttttat caacatgttt tagaggacaa acaatggact tatacctatt attaaaacga
     4441 aaaaatccca agctgcgtat gatatcgcaa cttgggattt tctgtattat ctactttgca
     4501 agtatgacgt tgggtctact gcatattgat taccgatgcc accagacata cgttggaagt
     4561 gtacgtgagg cgctgttgaa ttacccgtac tacctgaata tgcaatttgg tcaccagctt
     4621 tgactttatc accagctgaa acagttaaac gattattatg catataccat tggtagttat
     4681 tactgttcgc ttctttaatc gttacttgat tgccgccacc atagttactc caacctgctt
     4741 gtactactgt accatcagtt aatgagtaaa ctggtgaatt ttcaggcatt gcatagtcga
     4801 caccgtaatg cgcaccacca ccgtgatatt gtccatatgg ttgtagttgt ttacgacttg
     4861 ttaaccagct tgcgtctttc gcatgaccac tagctgtcgc tttacttgct gatccaccat
     4921 tttgattaga tgtgccatta ggataattga cctttccatt accatcatgg ctgttatacg
     4981 cttggttgtt gttactatgt gaataatagc tcgcgtctgg acctacattt gattgataac
     5041 catattgatt aatatgctgt tggctttgac tcgctgtgta gtcattgtta tctcctgctg
     5101 ttgctggatt cacatatgtt tggccgcttc cattggcatt tgcatttttt ggataacagt
     5161 tataaaaata atgcgtatgt ccttgagcat ctacgaatgt atagctatat tctttattat
     5221 caaacattgc ttgattccag ttaccatcag gtgtgtgatg ataatcccca ttagaatcaa
     5281 ttgtataata agtaccataa gatacgtctt gtgattgtgt tgacatttgt gtatgtgctt
     5341 gttgggtgtt tgtcgtttct gctgcatctg cttgatgcgc cattgtaaat gtagcgaagc
     5401 ccatcgttgc aatcgctgct gctgttaatt ttttcatgta taaaacatcc tccattaaag
     5461 ttaaagttag ttttcaatta aactgtactg cacatactaa aagaattaga caactgagta
     5521 aaggatttaa ttctcatttt ccaactattt aatattcccg aaatgtttta ctaaactcat
     5581 tacattgtca ttacaaaata gccatacatt gatattaaaa tgacatctct cactgcattc
     5641 gtttaacctt ttataaattt tcaaaattaa caactaatcg ttcgtccatg ttcgcgattc
     5701 aacgctaatg catgatagta atcatccatc aaatcatatc aaccaaattc cattatcaat
     5761 cgctattgat tgtcattcaa ctttctaata gtgatatgct tctcaggctt aaaaatcgtc
     5821 atatcttttc tattaattaa atcatctgtg agctttaatg ctactaattc attgctgcca
     5881 taatacttaa tatataacgt tcttgtagtt aaatttatta cggtctgata catcgtatag
     5941 tgattgtcag catcatgcgg acgtacaatt ccaatcggta tatttaccgc atctaataaa
     6001 taaaatgcat tcattaaatc catttcttta tcattgtttt gagcaatgtt tgctttcata
     6061 aatgccattc tcacaaagcg ctcagttgaa gtaaatccac ctggcaatcc aaatgtacct
     6121 gcttcattgc ctaaaggttc aatcgttaca ccttccaata aatttgctgt tgctggataa
     6181 ggagaaatat tgatatattg tcttaaatta ctataatgcc aatttaagtc tggatgattt
     6241 gttaagacac caataggatt atcttttata accacttcac cctctttaaa tgaaacttcg
     6301 actgtatgtc cagttgcatc ggaaacatga taatgcaatg gcggaacttc accgatgtca
     6361 tttaaatata cagctacaac atgtatttgg gatgcttgtt gtttcatatc ttcaatgctt
     6421 gttgtatatc ccaaaatcca tgtcacaatt tcattttgcg taatattcat cgcgtccgct
     6481 ttgtgtgttg atccatatga actataacct cggaaatatt gtgttgaaat ggcaacgcca
     6541 tgttcattaa caccatcacc ataaataaaa ccttccatat ctgttcctgt gccaataaag
     6601 ccatattgcg tttggcctgt cgtgccagtg cgagatttcc aacgataatt tctaggcgtc
     6661 actgctggcg aaccatctaa tggataatca taatccatcg tgcgtccaag aagtacttga
     6721 ttatttaaag tttgtattgt gaatcctgtg cacattgttc tcactcctct gtaccttcat
     6781 ttactttaat cactttcaaa taaagctgtt tcacttaaac atactataaa aaatcaatta
     6841 tacaagcaat taattgatat tcattctcaa taactgtggt atgatatgta aggaaatcat
     6901 gacttatgtg tgagtgaacg atcatctata catccgttca cttcatctca tgactttcta
     6961 tatttaattt ttacaaggag tgacatctgt gaataacaca caatcttcac cacgcagtaa
     7021 tattattatt gcgattatgt tgtctgcatt aacatattgg ttgtttgcac aatcatttat
     7081 taatatagga cctctcgttg gtcaaacgta tcaaacctct cctgccgtgt taaatttatc
     7141 tattagttta acttccttcg ccacaggtat cttcatggtg gctgcaggtg atattgctga
     7201 taaaatagga caactgagaa tgacatacat gggtctcata atcagtatgt ttgcatctct
     7261 tctattaatt atatcggaca tcactgcact gctcatcatc ggtagaattt tacaaggtct
     7321 atcagcagct atcttgttac cttcaacagt tggcgtgtta aataatcaat ttaaaggaga
     7381 acatttaaga cgagcgatta gttatctaat gattagtact gttggtggca tcggcctagc
     7441 tggtgttatc ggcggtttaa ttgcctcaaa tttcggatgg caaacgaatt tcatcattag
     7501 tatagtcatt gctttcattg ccatattgct tctaaaaggc acacctgaaa aagtaagtca
     7561 acatagccac cgtcatccat tcgattacaa aggtatgtcg attttcgctg ttatgattgg
     7621 tagctttaca ttattgttaa cacaaggatt cgaacaaggt tggtttagta cattttcaat
     7681 catttgtctg agcattttta tcatcactac gttgatattc atcatcatcg aacgtcgaca
     7741 tgaagtacct tttattgatt tctcagtatt acgcaaccgt ccgttcattg gtgcattttt
     7801 aaataacttt gttttaaata gcggtctagg cgtaacagtg gtctttttca tatatgctca
     7861 aacacacctt ggtttatcag ctgcgcaatc tggacttgtt acattgccat atgccattgt
     7921 ggcagttgcg atgattcgtt taggtgaaaa agcaacatta cgtttcggtg gcaaattgat
     7981 gctcatcatt ggtccgttgt tccctgtcat cggcatcact attattagca tgactcaatt
     8041 attggcatca caatatgtca ttgcagttat cattggtttc gtcatatgtg cgataggtaa
     8101 tggtttagtc gcaacacctg gacttacgat tgcaattttc agtatgccta atgaaaaagt
     8161 tggtttagct acaggattat ataaaatgag tggtacatta ggtggcgcct ttggtatagc
     8221 actaagtact acagttttca gtatgttaca actaaactat gcaccaagtg tagctgcaac
     8281 cgtaacattt atagtcagca ttgtattgat gatccttggc tcattgtctg catacatgat
     8341 cattccaaaa acagttaaat cttaaatata atagaagaat tatgtttcga aatatcttta
     8401 tcactttaaa atgatataca agaaatccaa gaaaaataag cgaactgaat aaataaagat
     8461 tcaattaacg catcagtatt aggattcact ctaaaacgat taatagtttt ataagaaggt
     8521 gtttgatctt gagctaacca catcattcga atactgtcat gaagtaattt ctctattcta
     8581 cgaccagaaa atacagattg agtatatgca tataagatga tttttaacat catctttgga
     8641 tgataggatg ttgcgccacg atgatgtctg aattcatcga atttgctatc aggtatcgtt
     8701 tcaacaattt cattaacatg tcgcgaaata tcattttgag gaattctaac agaagttttt
     8761 attggtagtg taagttgggc aaagtgtctt atttttttaa agtatttcaa agtaaaatta
     8821 catgttaata cgtagtatta atggcgagac t
//
LOCUS       DAKTIP010000107         8759 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.107, whole genome shotgun sequence.
ACCESSION   DAKTIP010000107 DAKTIP010000000
VERSION     DAKTIP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.107"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..648
                     /gene="capA"
                     /locus_tag="O0305_001789"
     CDS             <1..648
                     /gene="capA"
                     /locus_tag="O0305_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCY0274931.1"
                     /translation="TKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKYQANTQIL
                     VNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSKLSSMLTI
                     TNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKADGTAVKV
                     APKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSIQKFN"
     gene            664..1350
                     /gene="cap8B"
                     /locus_tag="O0305_001790"
     CDS             664..1350
                     /gene="cap8B"
                     /locus_tag="O0305_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCY0274932.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            1353..2117
                     /gene="cap8C"
                     /locus_tag="O0305_001791"
     CDS             1353..2117
                     /gene="cap8C"
                     /locus_tag="O0305_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCY0274933.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMMDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVINGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2137..3961
                     /gene="cap8D"
                     /locus_tag="O0305_001792"
                     /pseudo
     CDS             2137..3961
                     /gene="cap8D"
                     /locus_tag="O0305_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
     gene            3951..4979
                     /gene="cap8E"
                     /locus_tag="O0305_001793"
     CDS             3951..4979
                     /gene="cap8E"
                     /locus_tag="O0305_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCY0274934.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKQFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVYYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            4992..6101
                     /gene="cap8F"
                     /locus_tag="O0305_001794"
     CDS             4992..6101
                     /gene="cap8F"
                     /locus_tag="O0305_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCY0274935.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNTKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6105..7229
                     /gene="cap8G"
                     /locus_tag="O0305_001795"
     CDS             6105..7229
                     /gene="cap8G"
                     /locus_tag="O0305_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCY0274936.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGL
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7232..7858
                     /locus_tag="O0305_001796"
     CDS             7232..7858
                     /locus_tag="O0305_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCY0274937.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            7863..>8759
                     /locus_tag="O0305_001797"
     CDS             7863..>8759
                     /locus_tag="O0305_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0274938.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTDDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKEIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVIL"
BASE COUNT         3228 a         1203 c         1709 g         2619 t
ORIGIN      
        1 acaaaaatta aagaagtatt acaaaaaaac ttgaagattt taattatttt accgctatta
       61 tttttaatta ttagcgctat tgttacattt ttcgtcttat cacctaaata tcaagctaat
      121 actcaaattt tagtgaatca aactaagggt gacaatcctc agtttatggc gcaagaggtt
      181 caaagtaata ttcaacttgt aaatacgtat aaagaaattg ttaaaagtcc tagaatttta
      241 gatgaggtgt caaaggactt aaatgataag tattcaccat ctaaattgtc gagtatgttg
      301 acaattacaa accaagaaaa tacgcaactt atcaacatcc aagttaaaag tggtcataaa
      361 caagattcgg aaaaaattgc gaatagcttc gctaaagtta caagtaaaca aattccgaag
      421 attatgagtg tggataacgt atcaatttta tctaaagcag acggtacagc agttaaagtc
      481 gcaccaaaaa ctgtagtgaa tctaatcggt gcattctttt taggattagt tgtcgcgctt
      541 atatatatct tcttcaaagt aattttcgat aagcgaatta aagatgaaga agatgtagag
      601 aaagaattag gattgcctgt attgggttca attcaaaaat ttaattaagg atggttgcta
      661 cttatgtcaa aaaaggaaaa tacgacaaca acactatttg tatatgaaaa accaaaatca
      721 acaattagtg aaaagtttcg aggtatacgt tcaaacatca tgttttcaaa agcaaatggt
      781 gaagtaaagc gcttattggt tacttctgaa aagcctggtg caggtaaaag tacagttgta
      841 tcgaatgtag cgattactta tgcacaagca ggctataaga cattagttat tgatggcgat
      901 atgcgtaagc caacacaaaa ctatattttt aatgagcaaa ataataatgg actatcaagc
      961 ttaatcattg gtcgaacgac tatgtcagaa gcaattacgt cgacagaaat tgaaaattta
     1021 gatttgctaa cagctggccc tgtacctcca aatccatctg agttaattgg gtctgaaagg
     1081 ttcaaagaat tagttgatct gtttaataaa cgttacgaca ttattattgt cgatacaccg
     1141 ccagttaata ctgtgactga tgcacaacta tatgcgcgtg ctattaaaga tagtctgtta
     1201 gtaattgata gtgaaaaaaa tgataaaaat gaagttaaaa aagcaaaagc acttatggaa
     1261 aaagcaggca gtaacattct aggtgtcatt ttgaacaaga caaaggtcga taaatcttct
     1321 agttattatc actattatgg agatgaataa gtatgattga tattcataac catatattgc
     1381 ctaatatcga tgacggtccg acaaatgaaa cagagatgat ggatctttta aaacaagcga
     1441 caacacaagg tgttacagaa atcattgtaa catcacatca cttacatcct cgatatacca
     1501 cacctataga aaaagtgaaa tcatgtttaa accatattga aagcttagag gaagtacaag
     1561 cactaaatct aaagttttat tatggtcagg aaataagaat taccgatcaa atccttaatg
     1621 atattgatcg aaaagttatt aacggtatta atgattcacg ctatttacta atagaatttc
     1681 catcaaatga agttccacac tatactgatc aattattttt cgaattacag agtaaaggct
     1741 ttgtaccgat tattgcacat ccagagcgga ataaagcaat aagtcaaaac cttgacatac
     1801 tatacgattt aattaacaaa ggtgctttaa gtcaagtgac aacggcgtca ttagcgggta
     1861 tttccggtaa aaaaattaga aaattagcaa ttcaaatgat tgaaaacaat ctgacacatt
     1921 tcatcggttc agatgcgcat aacacagaaa tcagaccgtt cttaatgaaa gacttattta
     1981 atgataagaa attacgtgat tattatgaag atatgaacgg atttattagt aatgcgaagt
     2041 tagttgttga tgataaaaaa attcctaaac gaatgccaca acaagattat aaacagaaaa
     2101 gatggtttgg gttataaaca gcaaatgagg ggttttatgg cacatttatc tgtgaaattg
     2161 cggcttttaa tactagcatt aatcgattca ctgatagtga cattttcagt attcgtaagt
     2221 tattacattt tagaaccgta tttcaaaaca tattctgtca aattattaat attggcagct
     2281 atatcactat tcatatcgca tcatatttca gcatttattt ttaatatgta tcatcgagcg
     2341 tgggaatatg ccagtgtgag tgaattgatt ttaattgtta aagctgtgac gacatctatc
     2401 gttattacga tggtggtcgt gacaattgtt acaggcaata gaccgttttt tagattgtat
     2461 ttaattactt ggatgatgca cttgatttta ataggtggct caaggttatt ttggcgtatt
     2521 tatcggaaat accttggagg taagtcattt aataagaagc caactttagt tgttggtgct
     2581 ggtcaagcag gttcaatgct gattagacaa atgttgaaaa gtgacgaaat gaaacttgaa
     2641 ccggtattag cagtcgatga tgacgaacat aaacgcaata tcacaattac tgagggtgta
     2701 aaagtccaag gtaaaattgc ggatattcca gaactagtga ggaaatataa gattaaaaaa
     2761 atcatcattg caattccaac tattggtcaa gagcgtttga aagaaattaa taatatttgc
     2821 catatggatg gcgttgagtt attgaaaatg ccaaatatag aagacgtcat gtctggtgag
     2881 ttagaagtga accaacttaa aaaagttgaa gtagaagatt tactaggcag agatcctgtt
     2941 gaattagata tggatatgat atcaaatgaa ttgacgaata aaactatttt agttacgggt
     3001 gcaggtggtt caataggatc agaaatttgt agacaagttt gtaatttcta tccagaacgt
     3061 attattctac ttggccatgg tgaaaacagt atttatttaa tcaatcgtga attgcgaaat
     3121 cgcttcggaa aaaaatgttg atatcgttcc tattatagcg gatgtgcaaa atagagcgcg
     3181 tatgtttgaa attatggaaa cgtataaacc atacgcagtt tatcatgcag cagcacacaa
     3241 gcacgtgccg ttaatggaag acaaccctga agaagcagta cgtaataata ttttaggtac
     3301 gaaaaatact gctgaagctg ctaaaaatgc agaggtaaag aaattcgtta tgatttctac
     3361 ggataaagcc gttaatccgc ctaatgtcat gggagcttca aagcgaattg cagaaatgat
     3421 tattcaaagt ttaaatgatg aaacgcatcg aacaaatttt gttgcagtga gatttggtaa
     3481 tgtacttgga tcgagaggat ctgtgattcc acttttcaaa agtcaaattg aagaaggtgg
     3541 gccagttact gtgacacatc ctgaaatgac acgttacttt atgacaattc ctgaagcttc
     3601 tagactagtt ttgcaggcag gggcattagc agaaggtggc gaagtatttg tgctagatat
     3661 gggagaacca gtgaaaattg tagatttggc acgtaattta attaagctaa gtggtaaaaa
     3721 agaagacgac atacgcatta cttatacagg gattagaccc ggcgaaaaaa tgtttgaaga
     3781 gcttatgaat aaagatgagg ttcatcctga acaagtattt gaaaaaattt atcgtggcaa
     3841 agtacaacat atgaaatgta atgaagttga agcgattatt caagacatcg tcaatgactt
     3901 tagtaaagaa aaaattatta actatgccaa tggcaaaaag ggagataatt atgttcgatg
     3961 acaaaatttt attaattact gggggcacag gatcattcgg taatgctgtt atgaaacagt
     4021 ttttagattc taatattaaa gaaattcgta ttttttcacg cgatgagaaa aaacaagatg
     4081 acattcgaaa aaaatataat aattcaaaat taaagttcta cattggtgat gtgcgtgata
     4141 gtcaaagtgt agaaacagca atgcgagatg tttattacgt attccatgca gcagctttaa
     4201 aacaagtgcc gtcatgtgaa ttctttccag ttgaggcagt gaagacaaat attattggta
     4261 cagaaaatgt cttacaaagt gctattcatc aaaatgttaa aaaagtcata tgtttatcta
     4321 cagataaggc agcgtatcct attaatgcta tgggtatttc aaaagcaatg atggaaaaag
     4381 tattcgtagc caaatcaaga aatattcgta gtgaacaaac gcttatttgt ggtacaagat
     4441 acggtaatgt gatggcttca agaggatcag taataccttt gtttatcgac aaaatcaaag
     4501 ctggagaacc tttaacgatt acagatcctg atatgacaag atttttaatg agcttagaag
     4561 atgcggtaga actagttgtt catgcattta agcatgcaga gacaggagat attatggttc
     4621 aaaaagcacc aagctcaacg gtaggggatc ttgcgaccgc attattagaa ttgtttgaag
     4681 ctgataatgc aattgaaatc attggtacgc gacatggaga gaaaaaagca gaaacattgt
     4741 tgacgagaga agaatacgca caatgtgaag atatgggtga ttattttaga gtgccggcag
     4801 actccagaga tttaaattat agtaattatg ttgaaaccgg taacgaaaag attacgcaat
     4861 cttatgaata taactccgat aatacacata ttttaacggt ggaagagata aaagaaaaac
     4921 ttttaacact agaatatgtt agaaacgaat tgaatgatta taaagcttca atgagatagg
     4981 agagattgac gttgaatatt gtaattacag gagcaaaagg ttttgtagga aaaaacttga
     5041 aagcagattt aacttcaacg acagatcatc atattttcga agtacatcga caaactaaag
     5101 aggaagaatt agagtcagca ttgttgaaag cagactttgt cgtgcattta gcgggtgtta
     5161 atcgacctga acatgacaaa gaattcagct taggaaacgt gagttattta gatcatgtac
     5221 ttgatatatt aactagaaat acgaaaaagc cagcgatatt attatcgtct tcaatacaag
     5281 caacacaaga taatccttat ggtgagagta agttgcaagg ggaacagcta ttaagagagt
     5341 atgccgaaga gtatggcaat acggtttata tttatcgctg gccaaattta ttcggcaagt
     5401 ggtgtaagcc gaattataac tcagtgatag caacattttg ttacaaaatt gcacgtaacg
     5461 aagagattca agttaatgat cggaatgttg aactaacgct aaactacgtg gatgatatcg
     5521 tcgctgaaat aaagcgtgct attgaaggaa ctccaacgat tgaaaatggt gtacctacag
     5581 taccaaacgt atttaaagtg acattgggag aaattgtaga tttattatac aagttcaaac
     5641 agtcacgtct cgatcgaaca ttgccgaaat tagataactt gtttgaaaaa gatttgtata
     5701 gtacgtattt aagctatcta cctagtacag actttagtta tcccttactt atgaatgtgg
     5761 atgatagggg ttcttttaca gaatttataa aaacaccgga tcgtggtcaa gtttctgtaa
     5821 atatttctaa accaggtatt actaaaggta atcactggca tcatactaaa aacgaaaaat
     5881 ttctagtcgt atcaggtaaa ggggtaattc gttttagaca tgttaatgat gatgaaatca
     5941 ttgaatatta tgtttctggc gacaaattag aagttgtaga cataccagta ggatacacac
     6001 ataatattga aaatttaggc gacacagata tggtaactat tatgtgggtg aatgaaatgt
     6061 ttgatccaaa tcagccagat acgtatttct tggaggtata gcgcatggaa aaactgaaat
     6121 taatgacaat agttggtaca aggcctgaaa tcattcgttt atcatcaacg attaaagcat
     6181 gtgatcaata ttttaatcag atattagtac acactggtca aaattatgat tatacattga
     6241 atcaaatttt ctttgatgat ttggaattaa gacaaccgga ccactactta gaggcagttg
     6301 gaagtaacct tggagaaacg atggggaata ttattgcgaa gacatatgat gttttattac
     6361 gcgaacaacc agatgcactt ttaattcttg gtgatacaaa tagttgttta gcagcagtat
     6421 ctgctaaacg attaaagatt cctgtgttcc acatggaagc gggtaataga tgctttgatc
     6481 agaatgtacc tgaagaaatc aatcgtaaaa ttgttgacca tgtcagtgat gtgaatctac
     6541 cttatacgga acatagcaga cgttatttat tagatgaagg cctcaataaa gcgaatatct
     6601 ttgtgacagg atcaccgatg acagaagtga tagaagcgca tcgagataaa attaatcaca
     6661 gtgacgtttt aaataaacta ggattagaac cgcaacaata cattttagta tctgcgcata
     6721 gagaagagaa tatcgataat gaaaagaatt ttaaatcatt aatgaatgcg ataaatgata
     6781 ttgccaaaaa gtataaaatg cctgtgattt attcaacgca tccaagaagt tggaagaaaa
     6841 ttgaagaaag taaatttgaa tttgatccat tagttaaaca gttaaagcca tttggtttct
     6901 ttgattataa tgcattgcaa aaagatgcat ttgttgtgct atcagatagt ggaacattgt
     6961 cagaagagtc gtctattttg aagttccctg gtgtccttat tcgaacttcc acagaaagac
     7021 cggaagtact agataaaggt acggttattg taggtggtat tacctataac aatctaatcc
     7081 aatccgttga actagcaaga gagatgcaaa acaataacga accgatgatt gatgctattg
     7141 attataaaga cactaacgtt tcgacaaagg tagttaaaat tattcaaagc tataaagata
     7201 ttatcaatcg aaatacttgg aggaaatgac gatgaggata gcgattgaaa agataattgg
     7261 tttgctgaaa aaccagtcct ctaaagaatc gaatgttaag attcatcgct tggcgtatat
     7321 tacaaactca aaatttgatg gcaataacta tatagataga tggtgtaaaa tcaggaattc
     7381 tcacattggt gaatacagtt atattggatt tggtagtgat tttaataatg tagaagtagg
     7441 aagatattgt tcgatatctt cggatgtaaa aattgggtta ggaaaacatc ctacacactt
     7501 ttttagctca tcaccgattt tttattctaa taataatcca tttaacataa agcaaaagtt
     7561 tatagacttt aatgaccaac caagccgtac aacaattaaa aatgatgtgt ggattggtgc
     7621 aaatgtaatt attatggatg gtttaacaat aaatactggt gcagtcatag cagccggctc
     7681 agttgttact aaaaatgtag gagcatatga ggttgttggt ggtgttcctg caaaagtgat
     7741 taagaagcga tttgacaata aaacaattga aaaacttttg gaaagcaagt ggtgggagaa
     7801 aacgcctgac aaactaaaag gattttcggt tgaatattta aataaaaagg atacttaatg
     7861 atatgagaat tttaaatatt gtatcgagta atattgttca agacccaagg gtacttaaac
     7921 aaatagaaac aattaaaggc gttacggatg attataaaat tgttggaatg aataattcac
     7981 aagctactaa taagcgattg gaaaatttag attgtaatta tcgtttgtta ggtagcaagg
     8041 tagatccaaa aaatattctt tctaaattaa ttaagcgtat aagatttgca acaggtgtta
     8101 tccgagaaat taaagcttat aaacctgacg tgattcatgc aaatgatttc gacgtattat
     8161 taatggtcta tttaagcaat tataaaaaag ctaatattgt ttatgatgcg catgaaatat
     8221 atgcgaaaaa tgcctttatt aataaagttc cacttatttc aaagtttgta gaaagtatag
     8281 aaaaacacat agtaaaacat cgtgttaatg ccttcgtaac agtaagtcat gcagcaaaag
     8341 aatattatca atctaaagga tataagaagg aagcgaatgt tattacgaat gcacctattt
     8401 taaatgatag cagagaattt aaagaaatcg aaaactttaa agaaattgta tatcaaggtc
     8461 aaattgtaat ggacagagga tatgaagagt ttattattgc ttcatcagct tttaaacaaa
     8521 atgctccttc attcataatt cgagggtttg gtccgcatga agaagtgata aaagaactga
     8581 ttagttataa cccggaaaat attaggttgg ataaaccagt tgaagtaaaa gaattggttg
     8641 ataagttagc agaaagtaat gttggtgttg tcttgacgaa accagtatct attaattttg
     8701 aatatacagt atctaataaa atttttgaat gtatacatgc tggtttacca gtaatttta
//
LOCUS       DAKTIP010000108         8624 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAKTIP010000108 DAKTIP010000000
VERSION     DAKTIP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.108"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            34..786
                     /locus_tag="O0305_001798"
     CDS             34..786
                     /locus_tag="O0305_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCY0274939.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            complement(866..1510)
                     /locus_tag="O0305_001799"
     CDS             complement(866..1510)
                     /locus_tag="O0305_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCY0274940.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            1632..2375
                     /locus_tag="O0305_001800"
     CDS             1632..2375
                     /locus_tag="O0305_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCY0274941.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            2369..3205
                     /locus_tag="O0305_001801"
     CDS             2369..3205
                     /locus_tag="O0305_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCY0274942.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            3202..4131
                     /locus_tag="O0305_001802"
     CDS             3202..4131
                     /locus_tag="O0305_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCY0274943.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(5291..7333)
                     /locus_tag="O0305_001803"
     CDS             complement(5291..7333)
                     /locus_tag="O0305_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCY0274944.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(7673..8110)
                     /gene="mnhG2"
                     /locus_tag="O0305_001804"
     CDS             complement(7673..8110)
                     /gene="mnhG2"
                     /locus_tag="O0305_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCY0274945.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAEKREEHRKKWYQND"
     gene            complement(8085..8387)
                     /gene="mnhF2"
                     /locus_tag="O0305_001805"
     CDS             complement(8085..8387)
                     /gene="mnhF2"
                     /locus_tag="O0305_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCY0274946.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            complement(8384..>8624)
                     /gene="mnhE2"
                     /locus_tag="O0305_001806"
     CDS             complement(8384..>8624)
                     /gene="mnhE2"
                     /locus_tag="O0305_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCY0274947.1"
                     /translation="FKTKDMNPGLLSYETRLTSDWSITFLTILIIITPGSTVIRISQD
                     SKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
BASE COUNT         2982 a         1459 c         1270 g         2913 t
ORIGIN      
        1 aactttaaat taatatctta tatgaggtta acaatgaatt atttatcaaa cttttttaat
       61 acgacaattc aacttaatat atacattatc gttattgttg cagcttgtta tattgctatt
      121 catcaatata gacataagcc tgttttgaac tatttagatg tcattttaaa ctatatccct
      181 gttttaacac atgaatttgg tcatgtactg tttaataaac tcgctggtgg ccgtgcaaaa
      241 gatttagtca ttgtaacaag tccacgagag cgacaacaga cattacaaca aggattcgca
      301 attacacaat ctagacatct agcaggccaa tggcttacaa cgattggtgg ttattttatg
      361 ccacctatta tgttattgat aggtttagcg tcgagccatt atcagatacc tagtttcttt
      421 atatttacct atttattgat atttatttat tttttaattt taacatctag aaaaggctca
      481 cccattgttg ttataacttt aatttctatc atgttgtatt ttatattaaa agatgaaaat
      541 atagtagaaa ttcaactact tgtcacaatg agttatcaat atattttagg tgtattatta
      601 ggcgaagttc tacaatctag ttggacgatt gctaaattga ctttccagcg tccgagtcca
      661 cagtgggatg gtagtgcact taaagaactc agtcatgtac ctatcttcat ttatagtaca
      721 atctggatta tcttcaatct ttatgcagtg aatattttac tcaaatatac tcacttgata
      781 aattaactaa taaaaaagaa gcctaaagca attatccata tgtctttatc agttcaatca
      841 tggatatctt tatggcttct ttattttatt tttcagcaaa catgttcatt gcattttcgt
      901 aactgacaat aattacatta tcattacgtt taatgataat tactttattc atttcatctt
      961 tcgatacaat ttccacttca ttaccaatag aaatatcttt actagacaaa tatattagca
     1021 aatcggtctt atctctcaca cgtttgattg taacgatatc accaggttca taattcaaaa
     1081 tcgttgttat atatttctct ttatattcat tatttctagg aatcacaccg ccgtgcgggc
     1141 aagtttctgg gaaatttaac aggctatcca gcctttcaac aaataaatct gaaattcgat
     1201 gttctaaaat ttctgcttct tgatgtactt cttcccaatt atatttcaat atttctatta
     1261 aaaataattc taatagtcga tgtctcttaa tgatatcaag cgtatgcgtt aaaccatcct
     1321 ctgttaatct aacacctttg tatggttttg tttcaacata gcctgctttt tcaagacgtc
     1381 ctaccatttc acttacagat ggaggcttaa tatttaaaaa ttgagataag attttatttg
     1441 tcacaaagtt tttatcgcca ttattcgtaa ggattgcctt taaatagtcc tctttttctt
     1501 cagttaacat actttcacct cacatacatt gtctatatta tatcacgaat ttacttgaca
     1561 tgataaatat tctcagttta ttatacaatt aattaggtta gcctaaactt ttaattagga
     1621 ggtataaacg tttgttagaa acaaaagatt taaatctgtt tttaggtaat aagcatgtac
     1681 ttaaaaacat ttccttatcg ataccagtac gcggcgaaat aattggtatc atgggcccga
     1741 atggtgctgg taaatcttcc cttatcaagt ctttaattgg tgaatttaat gctaccggta
     1801 ctaaattgtt atataacaaa cctatacaac aacaactgca acatattaca tatattccac
     1861 aaaaagcaca tattgattta gattttccta taagtgtgga acaagtgatt ttatcaggtt
     1921 gctacaaaga aattggatgg tttagacgac ctaataaatc agcaagggat aaactcaaac
     1981 agttattaag cgatttagaa ttagaatctt tacgtcatcg acaaatttca gaattaagtg
     2041 gtggacaatt acaacgtgtg ctagtagcaa gagcattgat gtccgaaagt gaagtttatt
     2101 ttctagatga gccgtttgtc ggaattgatt ttagtagcga aaaattaatc atgacaaaaa
     2161 tcgagaactt aaaacaacaa ggaaaactta ttcttatcat ccaccatgat ctatcaaaag
     2221 caaagcaata ctttgatcgc attattctat taaatcaaac attacgatac tttggtgata
     2281 gtgaagaggc tatgagtgtc actcgcttaa acgaaacatt tatgagtagc actgactgta
     2341 gtgaccctag tcaaaggagc aatataacat gttagagttt gtcgaacatt tatttacata
     2401 tcaattcttg aatcgagcat tgataacttc aattattgta gggatagttt gtggtacagt
     2461 tggtagttta attgtattac gtggtctttc attaatggga gatgcaatga gtcacgcagt
     2521 attacctggt gttgccctat cattcttatt tggtattcca atgtttgtag gcgcacttat
     2581 aactggtatg atcgcaagta tttttatcgg ttatatcaca tctagtagta aaacgaaacc
     2641 tgacgccgct attggaatta gttttaccgc tttcttagct tcggggatta ttattattag
     2701 cttaataaac actacgacag atttgtacca tattctgttt ggtaatttac tggcaatcac
     2761 aaatagtgca tttttaacaa ctattgtgat tggttcaatc gtccttattc taatcattat
     2821 tttctatcgt ccattaatga tttctacatt cgatccaacg tttagtagaa tgagtggtct
     2881 aaatacgacg ttattacatt actttgtgat gttgttactc tcattagtaa cagtagcaag
     2941 tattcaaacg gtaggtatta ttcttgtagt tgcattatta attacacctg catctacagc
     3001 atttttaatt agtaaaaaac tatactcaat gatgattata gcaagtttaa taagtgtcat
     3061 aagttcaatt gtcggacttt attacagcta tatttacaac attcctagtg gtgcgacaat
     3121 cgtactttgt acgtttgtaa tttatatcat cacattattt ttcacaaaat ttacgaatag
     3181 aaagaaacga ggaagtttaa catgaaaaaa ttagtacctt tattattagc cttattactt
     3241 ctagttgctg catgtggtac tggtggtaaa caaagcagtg ataagtcaaa tggcaaatta
     3301 aaagtagtaa cgacgaattc aattttatat gatatggcta aaaatgttgg tggagacaac
     3361 gtcgatattc atagtattgt acctgttggt caagatcctc atgaatatga agttaaacct
     3421 aaagatatta aaaagttaac tgacgctgac gttattttat acaacggatt aaatttagag
     3481 actggtaacg gttggtttga aaaagcctta gaacaggctg gtaaatcatt aaaagataaa
     3541 aaagttatcg cagtatcaaa agatgttaaa cctatctatt taaacggtga agaaggcaac
     3601 aaagataaac aagatccaca cgcatggtta agtttagata acggtattaa atacgtaaaa
     3661 acaattcaac aaacatttat cgataacgac aaaaaacata aagcagatta tgaaaagcaa
     3721 ggtaacaaat acattgctca attggaaaaa ttaaataatg acagtaaaga caaatttaat
     3781 gacattccaa aagaacaacg tgccatgatt acaagtgaag gtgccttcaa gtacttctca
     3841 aaacaatacg gtattacacc aggttatatt tgggaaatta acactgaaaa acaaggtaca
     3901 cctgaacaaa tgagacaagc tattgagttt gttaaaaagc acaaattaaa acacttatta
     3961 gtagaaacaa gtgttgataa gaaagcaatg gaaagtttat ctgaagaaac gaagaaagat
     4021 atctttggtg aagtgtacac agattcaatc ggtaaagaag gcactaaagg tgactcttac
     4081 tacaaaatga tgaaatcaaa tattgaaact gtacacggaa gcatgaaata acacgctgtg
     4141 ttttaatgaa gtaagatgaa ttgatgttga tgcaacctaa aatattggta tctccaatat
     4201 tttaggccac acatcaacat aacaaagtcg aaggctaata gtcccatatc gtgcgttaaa
     4261 tatatattac cctcctatta atatatatac cgttcccgat cgcacgatat ggtggtatta
     4321 gaacttctct ttgaacgaaa gagaaaagct agaagttctt atgcagtttt aattaaactg
     4381 taaacatttg tcactcttta aatcaaagag taaagttaaa agctttatgt ggttttgatt
     4441 aaactgcgaa cagctgcttc tctttgaacg aaagagaaaa gctagaagtt cttatgcagt
     4501 tttaattaaa ctgtaaacat ttatcactct ttaaatcaaa gagtaaagtt aaaagcttta
     4561 tgtggttttg attaaactgc gaacagctgc ttctctttga acgagagaga aaagctagaa
     4621 gttcttatgc agttttaatt aaactgtcgt tcccttcatc tcttttaacc acagagatgc
     4681 gttagaagtt cttctaatac aatttataca acgccattcc ctacacactc ttataaaaga
     4741 gattcacgcg cgtcaataaa ttgtattaca tactaactaa aaagcttttc ttaatcgtac
     4801 taacgaagtt agaggttctt atgtgattgg acaactgttc tccctactag acttttagca
     4861 tagctggtta aaagtactat tacagattgt tcaattgtat gcccctttcc tttgaacgga
     4921 atattaaaag ctatgtgcag taacttttaa taggcatacc acttttgcat caagctataa
     4981 ataatcgcat actttgatat ttaatctggc ctatcaaagt aaacatctat ggtttcctcc
     5041 ccccatagat ccttaagcca cactcatccg atgtcatatg agtgtggcta ttttttgtga
     5101 atacacttaa cttcaccttt aaatgacttc gcttaatcta tctcctccaa tttttaaaac
     5161 ttatttcaat taaatctcaa atattaataa ttcgtatagt ttaaaactat ctattcaatt
     5221 cccctaaaat aaaagggatt gatgaaaagc ccttcagttt ttcatcaatc ccttttagca
     5281 taattaggaa ctacgtcata ctatctacaa taaccatttc atcataattg atggcttctc
     5341 ttagttttaa cgctgtacct tctgatactt catctgtatc aatcaactca cgtaaaatgc
     5401 gacgttgttc tctcaatgca tttaatttaa ttttagtaat cgtatcttca cttgctgagt
     5461 tgaagaaatt tgatggtgtt aagttttcaa ttcgcattaa ataaccgtca caaaccattc
     5521 caacttcaag tgtattatct tttgttgttt ccttagacaa acgactaacc acattataat
     5581 ggactaattt attaatttta tttaattcca ttaagttatc cgtaacacta agtgaagatg
     5641 cggagttcac tctcgtttgt actcttcgtt ttaataaagc acctcgtaat aatacaatca
     5701 ttcttcttat taatgatgct tgtctatata cctgtgttct ttcagcataa cgcatatagt
     5761 tttcaagtac actattcgtt atttgtcctt catctactaa agactctaat gtttttgttt
     5821 ctacattaaa agcaattttt tgtagacgtt ctaattcttt agagttttca tcatctttct
     5881 ctacagtttt taaaaatgct aatttatcat gatattcttt aatcacgtta ccatatttaa
     5941 aacttgtttc gaaagtagat ttttgattta gataatcaat aacttgttct aatatataaa
     6001 ttctagcaac tttaaacgac atattgccaa ttactgtttt aggtgcaggt ttcgttaata
     6061 atggcaataa tacttgcgca actaccaaac taataataac cataccagat gcaataaata
     6121 ataagtcgtt tctatacgta aaagcatgat gccctgctaa aaaatacggt aacgttaatg
     6181 cgattgctaa agaaattgtt ccatgcacac cacataacgt cataattaaa gcgtataaac
     6241 ttcgctttgg tggtttctca gtcgttggat tatcatcatc atttttagtc atcatttttt
     6301 ggaatggact gatggctaaa taaaaataag gatataagac ataaacccaa acaaatctaa
     6361 atagatagac agctaaagca acaacaatag tgatgcctat taaaaagatt aaattgtgcg
     6421 gttctgtttt gataatttta ataataactt caggtactaa aaatcctaat attgaaaaaa
     6481 caaagccatt taaaacataa cctagtatat tccatgtatg attgtaactc atttgcagtt
     6541 gtgtacgtac ttgcataatt ctgtcacgtt cgaaaccatg tacaagtcct gcaactactg
     6601 ctgcaatgat tcctgatgcg tgaaacaatt cagcaattaa atacgtaaca aatggtgtta
     6661 acaattgaat aattgtaaac atattaatgt tttcatatcc tcgacgcatc aatgttaatc
     6721 ggaaccttac taatgccata cctataagta aaccaaccac tgcgccacca attgatgcaa
     6781 ttaaaaacaa ctgaacagca tcaacaagtg aaaaagcacc tgtaactaat actccaacag
     6841 ctattttaaa tgaaataata ccagcagcat cattcaataa tgactcacct tcaagaattg
     6901 tcattgctcc ttttggcaag acctttcctt tagtgattgc ttgcactgct actgcatcag
     6961 taggacaaag aatggcagca attgcaaatg ctgctccaat aggtaaatct ggccaaatcc
     7021 aatgaataaa taaacctaca cctatcacag tagtaatgac taatcctaat gccatcatca
     7081 tcactggctt aatatatttc cttaaatgga ctctagaaac attaacacct tctacaaata
     7141 acaaaggcgc aatcattgtt accataaaca attcagaatc aaaattaaat tgaacaggga
     7201 ttggggtaat aaatagtaac atgcccaaga aaatttgtat aaatgctagg ggtactttag
     7261 gtatgaaagt atggacaaac gaacttagta tcacaacagc tataaatata agaattgttt
     7321 caaatatttc caaactttca cctctctaaa aagtattgtt taattgaaaa ttaagtatca
     7381 catctcgttg taattatact ttagaggata aattgagtta gcgaccacaa aagcacttta
     7441 atatagatat atgtctacga ttgcagtact taaatttgca attatttaat tttattttat
     7501 cactaattgt ttgtataaat aaacaacttg ctttcacata acaacattaa cttataatac
     7561 aaaaaatgag caccttaaaa tcgactaacc aatttcaaag tactctttta atgattaatt
     7621 ttgaaaacag attttcaaaa gcattgttat gcttaacaat ttagccaaca cttcaatcgt
     7681 tttgatacca tttcttacga tgctcttctc gtttttcagc acgtaattgt aatgcttctg
     7741 tagagttttg ttcatttgaa cttaataata ttgatgcatg tgtgtgagca tcattttttc
     7801 gatacatata agcgccgttg cgataagcag cgcgagcgac taagtgcatg ccgactggtg
     7861 aagttaaatt aataaaaaca agtgacagta ataaacgcac actgaaaaat cctgtattca
     7921 caataaaata aattaataca ccgattaaag ttaataacac ggataaagtt gaactttttg
     7981 tcgcagcgtg acttcttaag aaaacatctt ggaatttcac gatacctatt gcactaataa
     8041 gagcaataaa actacctaac aacaacatca cagcagcaat aagactaaag atttcttttg
     8101 ttatttccat taaacacatg ccccccacca ataaagcgtg atattgaaac agaacttaca
     8161 aaagatataa tggcaatgag catgattgaa tctaagaaag aaacggtgcc cataagtaca
     8221 cttaacacac ccacaattga cattacgaca gcacttgttg tatcaaatgt aacgacacga
     8281 tctgctgttg taggtccctt gattaatcta aataaacaga tgattaatgc aattccaaaa
     8341 ataatgagtg aactaataat cataatatgt gttattgttt gtatcatcgc gacacctcca
     8401 atattaagtc ttcataatgc ttaatacttc ttaacaaact atctttttct ttttctgaca
     8461 cgtcgatact atgaataaaa aactttttag agtcttgaga aattcgtatt actgtagacc
     8521 ctggagttat aataattaaa attgttaaaa atgttattga ccaatcactt gttagtcttg
     8581 tttcatatga aagtaatcca gggttcatat cttttgtttt aaaa
//
LOCUS       DAKTIP010000109         8515 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAKTIP010000109 DAKTIP010000000
VERSION     DAKTIP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.109"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            198..1151
                     /locus_tag="O0305_001807"
     CDS             198..1151
                     /locus_tag="O0305_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274948.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1314..1517)
                     /locus_tag="O0305_001808"
     CDS             complement(1314..1517)
                     /locus_tag="O0305_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0274949.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
     gene            1816..2304
                     /locus_tag="O0305_001809"
     CDS             1816..2304
                     /locus_tag="O0305_001809"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCY0274950.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2297..3232
                     /locus_tag="O0305_001810"
     CDS             2297..3232
                     /locus_tag="O0305_001810"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCY0274951.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNTLVGKKLI"
     gene            3229..4536
                     /gene="rsmB"
                     /locus_tag="O0305_001811"
     CDS             3229..4536
                     /gene="rsmB"
                     /locus_tag="O0305_001811"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCY0274952.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4539..5633
                     /gene="rlmN"
                     /locus_tag="O0305_001812"
     CDS             4539..5633
                     /gene="rlmN"
                     /locus_tag="O0305_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCY0274953.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5640..6383
                     /locus_tag="O0305_001813"
     CDS             5640..6383
                     /locus_tag="O0305_001813"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCY0274954.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6380..8374
                     /gene="pknB"
                     /locus_tag="O0305_001814"
     CDS             6380..8374
                     /gene="pknB"
                     /locus_tag="O0305_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCY0274955.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
BASE COUNT         3213 a         1178 c         1587 g         2537 t
ORIGIN      
        1 aaattaatgg ctataaacta ttttatatct atctatgttt taactactta aattattaga
       61 caaaacaatt ttcttaaatt atcaattaaa gtatttaaaa atattgatgg ttaatgtaaa
      121 atatcaatat aaataagttt tttaaattta tgtatgttta tttgattcaa acaaaataac
      181 ttaagaggag aaagtttatg aaaaagacac tgggatgttt acttttaatt atgcttttag
      241 tcgtagcagg ttgttctttt ggtgggaatc ataaattatc atcaaagaaa tcagaagaat
      301 caaaacaaga aactgtaaaa aaagaatcgg aagaagagaa agatccagat ttagagaaat
      361 atgaagaaat agagaagaaa atgaaaggaa ttaaagatgc gccatctctt gataagttgg
      421 atccattaat gacagaaaag tcgtttacga atagtaaagg gattcaagga tggaaagatt
      481 acaaagaatt aatgggtaaa gtggaacttg cagattatag atttactaaa gattcaaaag
      541 gatcttcaat aaaagatgtt gatgcattct ttaaaggtaa gaaaggtata aaaaggaaag
      601 tgattgaaac acacgatgat gtaaaacaag ttgattattg gtatgtagat ccagatggaa
      661 agaaaattgg caattcaaac acacctgttt tttacgcaga aattatgaca aaatataaag
      721 atggaaagtt agtttatgca tcagtcgaac caggatctta cgtaatacat aaagatgatg
      781 caattaaata tgacgattat tctaagttaa aaaaattaag tcagctaact aaacttgatc
      841 atccaaaacc agttccatat agcgtagctc aaatcaaatc tttcggagta cctttaacaa
      901 gcgtttcatt tatgacacat ggatcaaagg atactaaaga tgaagtgttg ccggcattgg
      961 cctatttcac tttttcacca aaaaattatg aagacaagtc taatccagat ccaaaagttt
     1021 taaatttagt acatatggat ttcttaaatg catctagtga ttttggtaac gcacattttg
     1081 ttgttttaag taaatatatt aaagagtatg aatcaaacta tgaaacagcg tcagatgatt
     1141 ctttaaaata gtatttactg tgtgaaaaat aaatagtgta ctacattaaa taatcgcaat
     1201 aataatcccg ataaacaatc agcattactg cttatcacat agagttcgta ataactataa
     1261 ctctatgatt cgcaaataat aaatgattgt catcgggatt tatttttatc aatttataaa
     1321 gtgacattac cttgttcatc agcaggtttg aaaacagtaa tcactgcact aataattgct
     1381 aaaatgtgtg ggatacctgt ccaacagaat attaagtgta gaataccttg catattcttg
     1441 ccggcataaa atttatgaat accaaaacta cctaagaaca atgctaataa aatataaata
     1501 actttgttta cttgcatttc tttccctcca gttgaattgc ttataatgac attagcttct
     1561 ctttttatta tacccacttt tagttcaaac attctagttt aagcattccc aatcatctaa
     1621 atttcagtta ttcaatcctt acaataaatt taggattaca tttcagttgc attgtattat
     1681 tttacgtgtg aaatatacgt aatgaatcac atgacaatct tcaaattgaa aaatatacat
     1741 tctatgatgt aaggtcgcat ttttaatata tttacgttat aatagtttga tgaattttat
     1801 tgaggagtaa gagttatggc gattaaaaag ttagtaccag catcgcatcc tattttaacg
     1861 aaaaaagcgc aagcagttaa aacatttgat gattcgttaa aaagattatt acaagattta
     1921 gaagatacaa tgtatgcaca agaagctgct ggcttatgtg cacctcaaat taatcagtca
     1981 ttgcaagtgg caattattga tatggaaatg gaaggattat tacaacttgt taatccgaaa
     2041 attattagtc aatcaaatga aacaataaca gacttagaag gttcaattac attgccagat
     2101 gtttacggcg aagtgacaag aagtaaaatg atagttgtcg aaagttatga cgtcaatggg
     2161 aacaaagttg aactaactgc acatgaagat gtagcaagaa tgattttgca tattatagat
     2221 caaatgaacg gtatcccttt tacagaacgt gcggaccgta ttttaacaga taaagaagtg
     2281 gaggcatatt ttataaatga ctaaaataat atttatgggt acaccagact tttcaacaac
     2341 tgttttagaa atgcttattg cagaacatga tgtcattgca gtcgtaacgc aaccagatcg
     2401 acctgttgga cgtaaacgtg ttatgacacc accaccagtt aaaaaagttg caatgaaata
     2461 tgatttacct gtatatcaac ctgaaaaatt aagtggatca gaagaattag aacaattgct
     2521 tcaattagat gtagatttaa ttgtaactgc tgcttttgga caattattac ctgaatcatt
     2581 gttggcatta ccaaatcttg gggcaattaa tgtacatgca tcattgttac cgaagtatag
     2641 aggtggtgca ccaattcatc aggcaattat cgatggtgaa caagaaaccg gcataacaat
     2701 tatgtatatg gttaaaaaat tagatgcggg taatattatt tcgcaacaag caattaaaat
     2761 agaagaaaat gataatgtcg gtacgatgca tgataaatta agtgtattag gggcagattt
     2821 attaaaagaa actttaccat ctattataga gggcacaaat gaaagcgtac ctcaagatga
     2881 tacgcaagca acatttgctt ccaatattcg acgcgaagat gagcgaatta gctggaataa
     2941 accaggaaga caagtgttta atcaaattcg tggattatca ccatggccag ttgcttatac
     3001 aactatggat gacactaact tgaaaatata cgatgctgaa ctcgttgaga ctaataagat
     3061 aaacgagcct ggaaccatta tagaaacgac taaaaaagcc attattgttg ctacaaatga
     3121 taatgaagct gttgcaatta aagatatgca attagctggg aaaaagagaa tgttagctgc
     3181 caattattta agtggtgcgc aaaacacact agtagggaag aaacttatat gatagaaaac
     3241 gtgagaagtc ttgcttttga cacgattcaa gatatattaa atgaaggtgc gtatagtaac
     3301 ttgcgtatca atgaagtgtt gtcagaaaat gaattaaatg caatggataa ggctttattt
     3361 acagaaattg tctacggaac cgttaaaaga aaatatacgt tagattttta tttaaagcct
     3421 tttgtgaaaa caaagattaa ggcatgggtt aggcaattat tatggatgag tatttatcaa
     3481 tatgtttatt tagataaagt tccaaatcat gccattatta atgaagcagt tgaaatagca
     3541 aaagaacgcg gtggctatca taatggtaat gtcgtaaatg gtattttacg tacaatgatg
     3601 cgtagtgact tacctgattt taatgaaatt gcagatccta aaaaaagaat ggcaatcgag
     3661 tatagtatgc cgaagtggat tatagatcat tgggcaacac attatggtct cgaagaaact
     3721 gaaacaattt tacagtcatt tttagaaacg acatcaacaa ctgtgcgtgc caacctgacg
     3781 cgagcatcat tagatgatat tattgaaaag ttgcaagacg aaggttatga cgttgaaaaa
     3841 gatcatgact taccttattg tctccatata ggaggacaac caattattca ttctcgttca
     3901 tttaaagatg gattcgtttc aattcaagat aaaagctcaa tgtttgttgc acacattatg
     3961 aatgtagacc gacatgatca cgtattagat gcatgtagtg cacctggcgg taaagcttgt
     4021 cacattgctg aagttttaat gccagaaggg caagttgacg cttcagatat acatgatcac
     4081 aaaatagact taattaattt taatataaaa aaattacgat taacaaatat taaagctttt
     4141 caacatgatg cgacaaaacc ttatgataaa acatacgata agatacttgt tgatgcacca
     4201 tgtagcggat taggtgtaat gagacataag ccggagatta agtatactca aagcaaacaa
     4261 catattgagt cactagttga attacagctt gaaatattgg aaaatgtaaa aaacaatgta
     4321 aaaataggtg gcgaaatcat ctattcaaca tgtacaattg agcaactaga aaatgaaaac
     4381 gtgatttata cgtttttgaa aaataataaa aacttcgaat ttgaaccgtt tcaacatccg
     4441 ataactggag agttggtcaa aacgttacaa atcatgccgc aagactttaa ttcagatgga
     4501 ttctttatca ctaagataaa aagaaaggac aattaggaat gataactgct gaaaagaaaa
     4561 agaagaataa atttcttcca aattttgaca agcaatcaat atattcattg cgatttgacg
     4621 aaatgcaaaa ctggctcgtt gaacaagggc aacaaaaatt tcgagcgaaa cagatttttg
     4681 aatggttata tcaaaaaaga gtagattcga ttgatgaaat gacgaactta tcgaaagact
     4741 tacgacagct tttaaaagat aactttactg ttacaacttt aacaactgta gtaaaacaag
     4801 aaagtaaaga cggtacaatt aaattcttat ttgaattaca agatggctat acaattgaaa
     4861 ctgttttaat gagacatgat tatggaaatt cagtatgtgt aacgacacaa gtaggttgtc
     4921 gcatcggatg tacgttttgt gcttctacac ttggcggctt aaaaagaaac cttgaagctg
     4981 gcgaaattgt ttcacaagtt ttaacagttc aaaaagccct tgatgctaca gaagagcgcg
     5041 tatctcaaat tgtcataatg ggtatcggtg aaccatttga aaattatgat gaaatgatgg
     5101 actttttaag aatcgtcaat gatgataaca gtttaaatat tggtgcacgt cacattacag
     5161 tatcaacatc aggtatcatt cctagaatat atgactttgc ggatgaagat attcaaatta
     5221 attttgctgt aagcttacac gccgcaaaag atgaagtgcg atcacgcttg atgccaatta
     5281 accgtgcata taatgttgag aagttaatcg aagcaattca atattatcaa gaaaaaacaa
     5341 atcgtcgtgt tacttttgaa tatggtctgt ttggtggtgt gaatgaccaa ctagaacatg
     5401 caagagaatt agcacattta ataaaaggct taaactgcca tgttaactta attcctgtca
     5461 accatgttcc agaaagaaat tatgtgaaaa cggctaaaaa tgatatcttt aaatttgaaa
     5521 aagaattaaa gagactagga attaatgcca caatacgtcg tgaacaaggt tcggatattg
     5581 acgcagcttg tggtcaatta agagcaaagg aacgacaagt agaaacgagg taaagacaaa
     5641 tgctagaggc acaatttttt actgatactg gacaacatag agataagaat gaagatgcgg
     5701 gtggtatttt ttataatcaa actaatcaac aacttttagt tctgtgtgat ggtatgggtg
     5761 gccataaagc aggagaagtt gcaagtaaat ttgttacaga tgagttgaaa tcccgttttg
     5821 aagcggaaaa tcttatagaa caacatcaag ctgaaaattg gttgcgtaat aatataaaag
     5881 atataaattt tcagttatat cactatgcac aagaaaatgc agaatataaa ggtatgggta
     5941 caacatgtgt ttgtgcactt gtttttgaaa aatcagttgt gatagcaaat gtcggtgatt
     6001 ctagagccta tgttattaat agtcgacaaa ttgaacaaat tactagtgat cactcatttg
     6061 ttaatcatct tgttttaacg ggtcaaatta cgccggaaga agcatttaca catccacaac
     6121 gtaatattat tacgaaggtg atgggcacag ataaacgtgt gagtccagat ttgtttatta
     6181 agcgattaaa tttttatgat tatttattat taaattcaga tggattaact gattatgtta
     6241 aagacaatga aattaagcgt ttgttagtaa aagaaggtac aatagaagat catggtgatc
     6301 aattaatgca attggcatta gataaccatt cgaaagataa cgttactttc atactcgcgg
     6361 ctattgaagg tgataaagta tgataggtaa aataataaat gaacgatata aaattgtaga
     6421 taagcttggc ggcggtggca tgagtaccgt ttatcttgct gaagatacga tacttaacat
     6481 taaagttgca attaaggcga tttttatacc acctagagaa aaagaagaaa cattaaaacg
     6541 ttttgaacga gaagtacata actcatcaca gctatcacat caaaatatag taagtatgat
     6601 cgatgttgat gaagaagatg actgttacta cttagtaatg gaatatatcg aaggtccgac
     6661 tttgtctgag tatattgaaa gtcatgggcc attaagtgtt gacacagcga ttaattttac
     6721 gaatcaaata ttggatggca ttaaacatgc gcatgatatg cgtattgtac atagagatat
     6781 taagccacaa aatatattaa ttgacagcaa taaaacgttg aaaatatttg attttggaat
     6841 tgctaaagct ttaagtgaga cgtctttaac tcagactaat catgtgttag gtactgtgca
     6901 gtacttttcg ccagaacaag caaaaggtga ggcaacggat gaatgtacag atatttattc
     6961 tataggtatt gtgttatatg aaatgcttgt tggtgaacca ccctttaatg gagaaactgc
     7021 agttagcatt gcgattaaac atattcagga ttctgtgcca aatgtgacaa cagatgtacg
     7081 taaggatatt ccgcaatctt taagtaatgt cattttacgc gctacagaaa aagacaaagc
     7141 gaatcgttac aaaacaattc aagaaatgaa agatgatttg agtagtgttt tacatgaaaa
     7201 tcgagcgaat gaagatgtct atgaactcga taaaatgaaa acgatagcgg tacctttgaa
     7261 aaaagaagat ctagcaaagc atattagtga acataagtcg aatcaaccta aacgtgaaac
     7321 gacgcaagta cctattgtaa atgggcctgc tcatcatcag caattccaaa agccagaagg
     7381 tacggtgtac gaaccaaaac ctaaaaagaa atcaacacga aagattgtgc tcttatcact
     7441 aatcttttcg ttgttaatga ttgcacttgt ttcttttgtg gcaatggcaa tgtttggtaa
     7501 taaatacgaa gagacacctg atgtaatcgg gaaatctgta aaagaagcag agcaaatatt
     7561 caataaaaac aacctgaaat tgggtaaaat ttctagaagt tatagtgata aatatcctga
     7621 aaatgaaatt attaagacaa ctcctaatac tggtgaacgt gttgaacgtg gtgacagtgt
     7681 tgatgttgtt atatcaaagg gccctgaaaa ggttaaaatg ccaaatgtca ttggtttacc
     7741 taaggaggaa gccttgcaga aattaaaatc gttaggtctt aaagatgtta cgattgaaaa
     7801 agtatataat aatcaagcgc caaaaggata cattgcaaat caaagtgtaa ccgcaaatac
     7861 tgaaatcgct attcatgatt ctaatattaa actatatgaa tctttaggca ttaagcaagt
     7921 ttatgtagaa gactttgaac ataaatcctt tagcaaagct aaaaaagcct tagaagaaaa
     7981 agggtttaaa gttgaaagta aggaagagta tagtgacgat attgatgagg gtgatgtgat
     8041 ttctcaatct cctaaaggaa aatcagtaga tgaggggtca acgatttcat ttgttgtttc
     8101 taaaggtaaa aaaagtgact catcagatgt caaaacgaca actgaatcgg tagatgtacc
     8161 atacactggt aaaaatgata agtcacaaaa agttaaagtt tatattaaag ataaagataa
     8221 tgacggttca actgaaaaag gtagtttcga tattactagt gatcaacgta tagacattcc
     8281 tttaagaatt gaaaaaggaa aaacagcaag ttatattgtt aaagttgacg gtaaaactgt
     8341 agctgaaaaa gaagtcagct atgatgatgt ataaatataa ttgaagtaaa tgtaccgagg
     8401 tttctatttg gaagtctcgg tatttttatg ttgagattgc ggtagtttta aaatgcttca
     8461 tgtcatcata tacaggtgag cattgtctat caattctgta tcactctcac aatat
//
LOCUS       DAKTIP010000110         8444 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAKTIP010000110 DAKTIP010000000
VERSION     DAKTIP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.110"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            3..1133
                     /gene="dnaN"
                     /locus_tag="O0305_001815"
     CDS             3..1133
                     /gene="dnaN"
                     /locus_tag="O0305_001815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017723676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCY0274956.1"
                     /translation="MEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVIL
                     TGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQF
                     QTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETRP
                     VLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDNE
                     EDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAIDR
                     ASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYMM
                     DALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            1514..1759
                     /gene="yaaA"
                     /locus_tag="O0305_001816"
     CDS             1514..1759
                     /gene="yaaA"
                     /locus_tag="O0305_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCY0274957.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            1756..2868
                     /gene="recF"
                     /locus_tag="O0305_001817"
     CDS             1756..2868
                     /gene="recF"
                     /locus_tag="O0305_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCY0274958.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            2878..4812
                     /gene="gyrB"
                     /locus_tag="O0305_001818"
     CDS             2878..4812
                     /gene="gyrB"
                     /locus_tag="O0305_001818"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCY0274959.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            4849..7512
                     /gene="gyrA"
                     /locus_tag="O0305_001819"
     CDS             4849..7512
                     /gene="gyrA"
                     /locus_tag="O0305_001819"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCY0274960.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFENLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEE"
     gene            complement(7599..8411)
                     /locus_tag="O0305_001820"
     CDS             complement(7599..8411)
                     /locus_tag="O0305_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCY0274961.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         2947 a         1300 c         1681 g         2516 t
ORIGIN      
        1 tgatggaatt cactattaaa agagattatt ttattacaca attaaatgac acattaaaag
       61 ctatttcacc aagaacaaca ttacctatat taactggtat caaaatcgat gcgaaagaac
      121 atgaagttat attaactggt tcagactctg aaatttcaat agaaatcact attcctaaaa
      181 ctgtagatgg cgaagatatt gtcaatattt cagaaacagg ctcagtagta cttcctggac
      241 gattctttgt tgatattata aaaaaattac ctggtaaaga tgttaaatta tctacaaatg
      301 aacaattcca gacattaatt acatcaggtc attctgaatt taatttaagt ggcttagatc
      361 cagatcaata tcctttatta cctcaagttt ctagagatga cgcaattcaa ttgtcggtaa
      421 aagtgcttaa aaacgtgatt gcacaaacaa attttgcagt gtccacctca gaaacacgcc
      481 cagtactaac tggtgtgaac tggcttatac aagaaaatga attaatatgc acagcgactg
      541 actcacaccg cttggctgta agaaagttgc agttagaaga tgtttctgaa aacaaaaatg
      601 tcatcattcc aggtaaggct ttagctgaat taaataaaat tatgtctgac aatgaagaag
      661 acattgatat cttctttgct tcaaaccaag ttttatttaa agttggaaat gtgaacttta
      721 tttctcgatt attagaagga cattatcctg atacaacacg tttattccct gaaaactatg
      781 aaattaaatt aagtatagac aatggggagt tttatcatgc gattgatcgt gcctctttat
      841 tagcgcgtga aggtggtaat aacgttatta aattaagtac aggtgatgac gttgttgaat
      901 tgtcttctac atcaccagaa attggtactg taaaagaaga agttgatgca aacgatgttg
      961 aaggtggtag cctgaaaatt tcattcaact ctaaatatat gatggatgct ttaaaagcaa
     1021 tcgataatga tgaggttgaa gttgaattct tcggtacaat gaaaccattt attctaaaac
     1081 caaaaggtga cgactcggta acgcaattaa ttttaccaat cagaacttac taaaaataaa
     1141 tataaataaa ggatgacgtg attaattaaa acgtcatcct ttattttttg gcaaaaataa
     1201 ttctaggtgc gtatgtaaaa taaatttggc agcattttaa acagcaaata aaagacgcca
     1261 attaaattta tgacaaatgt atccaaaatt taataagtgt gcttatatgc cctttaaatt
     1321 taaaatttta atagtcaata acaagttgaa tataaaagtt aaacgccgtt aaatagcgtt
     1381 aaaaaattga aaatgacagt attgccaaaa aataagaatt aattatttat atgtaaacgg
     1441 tttctacctc tattttaaat gaaatttgtg acaaaaaaag gtataatata ttaatgacat
     1501 acaaagaaat ggagtgatta ttttggttca agaagttgta gtagaaggag acattaattt
     1561 aggtcaattt ctaaaaacag aagggattat tgaatctggt ggtcaagcaa aatggttctt
     1621 gcaagacgtt gaagtattaa ttaatggagt gcgtgaaaca cgtcgcggta aaaagttaga
     1681 acatcaagat cgtatagata tcccagaatt acctgaagat gctggttctt tcttaatcat
     1741 tcatcaaggt gaacaatgaa gttaaataca ctccaattag aaaattatcg taactatgat
     1801 gaggttacgt tgaaatgtca tcctgacgtg aatatcctca ttggagaaaa tgcacaagga
     1861 aagacaaatt tacttgaatc aatttatacc ttagctttag caaaaagtca tagaacgagt
     1921 aatgataagg aactcatacg ttttaatgct gattatgcta aaatagaagg tgagcttagt
     1981 tatagacacg gcacgatgcc attaacaatg tttataacta aaaaaggtaa acaagtcaaa
     2041 gtgaatcact tagagcaaag tcgtctaact caatatattg gacacctcaa tgtggttcta
     2101 tttgcgccag aagatttgaa tattgtaaaa ggctctcctc aaataagacg acgctttata
     2161 gatatggagt tgggccaaat ttctgctgtt tacttaaatg atttagctca ataccaacgt
     2221 attttaaagc aaaagaataa ttacttaaag cagttacaat taggccaaaa aaaggactta
     2281 acaatgttgg aagtattaaa tcagcagttt gctgaatatg caatgaaagt aactgataaa
     2341 cgtgcacatt ttattcaaga gctagagtcg ttagctaaac cgattcatgc tggtatcaca
     2401 aatgataaag aagcgttgtc gctgaattat ttacctagtc ttaaatttga ttatgctcaa
     2461 aatgaagcgg cacgacttga agaaattatg tctattctta gcgataatat gcaaagagaa
     2521 aaagaacgag gcattagctt attcggacca catcgagatg atataagttt tgatgtgaat
     2581 ggcatggatg ctcaaacata tggttctcaa ggacagcaac gtacaacggc tttgtccatt
     2641 aaattagctg aaattgagtt aatgaatatc gaagttgggg aatatcccat cttattatta
     2701 gacgatgtac tcagtgaatt agatgattcg cgtcaaacgc atttattaag tacgattcag
     2761 cataaagtac aaacatttgt cactacgaca tctgtagatg gtattgatca tgaaatcatg
     2821 aataacgcta aattgtatcg tattaatcaa ggtgaaatta taaagtaaca gaaagcgatg
     2881 gtgactgcat tgtcagatgt aaacaacacg gataattatg gtgctgggca aatacaagta
     2941 ttagaaggtt tagaagcagt acgtaaaaga ccaggtatgt atataggatc gacttcagag
     3001 agaggtttgc accatttagt gtgggaaatt gtcgataata gtatcgatga agcattagct
     3061 ggttatgcaa atcaaattga agttgttatt gaaaaagata actggattaa agtaacggat
     3121 aacggacgtg gtatcccagt tgatattcaa gaaaaaatgg gacgtccagc tgtcgaagtt
     3181 attttaactg ttttacatgc tggtggtaaa tttggcggtg gcggatacaa agtatctggt
     3241 ggtttacatg gtgttggttc atcagttgta aacgcattgt cacaagactt agaagtatat
     3301 gtacacagaa atgagactat atatcatcaa gcatataaaa aaggtgtacc tcaatttgac
     3361 ttaaaagaag ttggcacaac tgataagaca ggtactgtca ttcgttttaa agcagatgga
     3421 gaaatcttca cagagacaac tgtatacaac tatgaaacat tacagcagcg tattagagag
     3481 cttgctttct taaacaaagg aattcaaatc acattaagag atgaacgtga tgaagaaaac
     3541 gttagagaag actcctatca ctatgagggc ggtattaaat cgtacgttga gttattgaac
     3601 gaaaataaag aacctattca tgatgagcca atttatattc atcaatctaa agatgatatt
     3661 gaagtagaaa ttgcgattca atataactca ggatatgcca caaatctttt aacttacgca
     3721 aataacattc atacgtatga aggtggtacg catgaagacg gattcaaacg tgcattaacg
     3781 cgtgtcttaa atagttatgg tttaagtagc aagattatga aagaagaaaa agatagactt
     3841 tctggtgaag atacacgtga aggtatgaca gcaattatat ctatcaaaca tggtgatcct
     3901 caattcgaag gtcaaacgaa gacaaaatta ggtaattctg aagtgcgtca agttgtagat
     3961 aaattattct cagagcactt tgaacgattt ttatatgaaa atccacaagt cgcacgtaca
     4021 gtggttgaaa aaggtattat ggcggcacgt gcacgtgttg ctgcgaaaaa agcgcgtgaa
     4081 gtaacacgtc gtaaatcagc gttagatgta gcaagccttc caggtaaatt agccgattgc
     4141 tctagtaaaa gtcctgaaga atgtgagatt ttcttagtcg aaggggactc tgccgggggg
     4201 tctacaaaat ctggtcgtga ctctagaacg caggcgattt taccattacg aggtaagata
     4261 ttaaatgttg aaaaagcacg attagataga attttgaata acaatgaaat tcgtcaaatg
     4321 atcacagcat ttggtacagg aatcggtggc gactttgatc tagcgaaagc aagatatcac
     4381 aaaatcgtca ttatgactga tgccgatgtg gatggagcgc atattagaac attgttatta
     4441 acattcttct atcgatttat gagaccgtta attgaagcag gctatgtgta tattgcacag
     4501 ccaccgttgt ataaactgac acaaggtaaa caaaagtatt atgtatacaa tgatagggaa
     4561 cttgataaac ttaaatctga attgaatcca acaccaaaat ggtctattgc acgatacaaa
     4621 ggtcttggag aaatgaatgc agatcaatta tgggaaacaa caatgaaccc tgagcaccgc
     4681 gctcttttac aagtaaaact tgaagatgcg attgaagcgg accaaacatt tgaaatgtta
     4741 atgggtgacg ttgtagaaaa ccgtagacaa tttatagaag ataatgcagt ttatgcaaac
     4801 ttagacttct aagcgctgtg aactgaactt ttgaaggagg aactcttgat ggctgaatta
     4861 cctcaatcaa gaataaatga acgaaatatt accagtgaaa tgcgtgaatc atttttagat
     4921 tatgcgatga gtgttatcgt tgctcgtgca ttgccagatg ttcgtgacgg tttaaaacca
     4981 gtacatcgtc gtatactata tggattaaat gaacaaggta tgacaccgga taaatcatat
     5041 aaaaaatcag cacgtatcgt tggtgacgta atgggtaaat atcaccctca tggtgactca
     5101 tctatttatg aagcaatggt acgtatggct caagatttca gttatcgtta tccgcttgtt
     5161 gatggccaag gtaactttgg ttcaatggat ggagatggcg cagcagcaat gcgttatact
     5221 gaagcgcgta tgactaaaat cacacttgaa ctgttacgtg atattaataa agatacaata
     5281 gattttatcg ataactatga tggtaatgaa agagagccgt cagtcttacc tgctcgattc
     5341 cctaacttat tagccaatgg tgcatcaggt atcgcggtag gtatggcaac gaatattcca
     5401 ccacataact taacagaatt aatcaatggt gtacttagct taagtaagaa ccctgatatt
     5461 tcaattgctg agttaatgga ggatattgaa ggtcctgatt tcccaactgc tggacttatt
     5521 ttaggtaaga gtggtattag acgtgcatat gaaacaggtc gtggttcaat tcaaatgcgt
     5581 tctcgtgcag ttattgaaga acgtggaggc ggacgtcaac gtattgttgt cactgaaatt
     5641 cctttccaag tgaataaggc tcgtatgatt gaaaaaattg cagagctcgt tcgtgacaag
     5701 aaaattgacg gtatcactga tttacgtgat gaaacaagtt tacgtactgg tgtgcgtgtc
     5761 gttattgatg tgcgtaagga tgcaaatgct agtgtcattt taaataactt atacaaacaa
     5821 acacctcttc aaacatcatt tggtgtgaat atgattgcac ttgtaaatgg tagaccgaag
     5881 cttattaatt taaaagaagc gttggtacat tatttagagc atcaaaagac agttgttaga
     5941 agacgtacgc aatacaactt acgtaaagct aaagatcgtg cccacatttt agaaggatta
     6001 cgtatcgcac ttgaccatat cgatgaaatt atttcaacga ttcgtgagtc agatacagat
     6061 aaagttgcaa tggaaagctt gcaacaacgc ttcaaacttt ctgaaaaaca agctcaagct
     6121 attttagaca tgcgtttaag acgtctaaca ggtttagaga gagacaaaat tgaagctgaa
     6181 tataatgagt tattaaatta tattagtgaa ttagaagcaa tcttagctga tgaagaagtg
     6241 ttattacagt tagttagaga tgaattgact gaaattagag atcgtttcgg tgatgatcgt
     6301 cgtacagaaa ttcaattagg tggatttgaa aacttagagg acgaagactt aattccagaa
     6361 gaacaaatag taattacact aagccataat aactacatta aacgtttgcc ggtatctaca
     6421 tatcgtgctc aaaaccgtgg tggtcgtggt gttcaaggta tgaatacatt ggaagaagat
     6481 tttgtcagtc aattggtaac tttaagtaca catgaccatg tattgttctt tactaacaaa
     6541 ggtcgtgtat acaaacttaa aggttacgaa gtgcctgagt tatcaagaca gtctaaaggt
     6601 attcctgtag tgaatgctat tgaacttgaa aatgatgaag tcattagtac aatgattgct
     6661 gttaaagacc ttgaaagtga agacaacttc ttagtgtttg caactaaacg tggtgtcgtt
     6721 aaacgttcag cattaagtaa cttctcaaga ataaatagaa atggtaagat tgcgatttcg
     6781 ttcagagaag atgatgagtt aattgcagtt cgcttaacaa gtggtcaaga agatatcttg
     6841 attggtacat cacatgcatc attaattcga ttccctgaat caacattacg tcctttaggc
     6901 cgtacagcaa cgggtgtgaa aggtattaca cttcgtgaag gtgacgaagt tgtagggctt
     6961 gatgtagctc atgcaaacag tgttgatgaa gtattagtag ttactgaaaa tggttatggt
     7021 aaacgtacgc cagttaatga ctatcgctta tcaaatcgtg gtggtaaagg tattaaaaca
     7081 gctacgatta ctgagcgtaa tggtaatgtt gtatgtatca ctacagtaac tggtgaagaa
     7141 gatttaatga ttgttactaa tgcaggtgtc attattcgac tagatgttgc agatatttct
     7201 caaaatggtc gtgcagcaca aggtgttcgc ttaattcgct taggtgatga tcaatttgtt
     7261 tcaacggttg ctaaagtaaa agaagatgca gaagatgaaa cgaatgaaga tgagcaatct
     7321 acttcaactg tatctgaaga tggtactgaa caacaacgtg aagcggttgt aaatgatgaa
     7381 acaccaggaa atgcaattca tactgaagtg attgattcag aagaaaatga tgaagatgga
     7441 cgtattgaag taagacaaga tttcatggat cgtgttgaag aagatataca acaatcatca
     7501 gatgaagaat aataaaaaat aagacttccc tatatgtagg ggagtcttat ttttatgcta
     7561 gaaagtaatg ctttactata ttcaatgatt agtaatgact aactttctaa ttgtttcatt
     7621 gcgtaaggta tttcattgat aagtcttgat ggtggcacca catacatatc ttttgcaagg
     7681 ttttcgccaa taaaactatg tgtatatgtg gcactcataa ccgcttcttt taagttatca
     7741 aattgaccga caaagcttgt aatcatacca gcaagtgtat cgcccatacc accagtcgcc
     7801 attgctgggc taccgattgt caatttaaag tcttcatctt taaagaaaat ttcagtacca
     7861 tgttttttaa gtacaacagt tgcacctaaa cgatcaactg cttcacgatt acgctcatat
     7921 gtctgttcct caataggaat accacttaat cgttcccatt ctttgaggtg tggtgtaaag
     7981 atcacacgac atgtaggtaa ttgcggtttc agtttactaa agattgtaat cgcatcgccg
     8041 tctacgatta aattttgatg cggttgtata ttttgtagta ggaatgtaat ggcattattt
     8101 cctttgaaat caacgccaag acctggacca attagtatac tgtcagtcat ttcaatcatt
     8161 ttcgtcaaca ttttcgtatc attaatatca ataaccatcg cttctgggca acgagaatgt
     8221 aatgctgaat gatttgttgg atgtgtagct acagtgatta aaccactacc gctaaataca
     8281 catgcacgag ccgctaacat aatggcacca cctaagttag cagatccacc aattaataaa
     8341 attttgccat aatcaccttt atgtgaatct tctttacgct taggaatgtt aatagaattt
     8401 aacgtttcca tagtgatata acctcccatg taaaagcctt ttcc
//
LOCUS       DAKTIP010000111         8389 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAKTIP010000111 DAKTIP010000000
VERSION     DAKTIP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.111"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..430
                     /gene="crtO"
                     /locus_tag="O0305_001821"
     CDS             <1..430
                     /gene="crtO"
                     /locus_tag="O0305_001821"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCY0274962.1"
                     /translation="NIANLGTRIPDKYFRQKYIIFKSFNFEKHGKFWNKWFYVRKWKH
                     KILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAELIHWISILPVIIFNKGPRLVK
                     YINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGERHD"
     gene            423..1916
                     /locus_tag="O0305_001822"
     CDS             423..1916
                     /locus_tag="O0305_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0274963.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            1922..3049
                     /gene="crtQ"
                     /locus_tag="O0305_001823"
     CDS             1922..3049
                     /gene="crtQ"
                     /locus_tag="O0305_001823"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCY0274964.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            3086..3949
                     /locus_tag="O0305_001824"
     CDS             3086..3949
                     /locus_tag="O0305_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCY0274965.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            3961..5469
                     /locus_tag="O0305_001825"
     CDS             3961..5469
                     /locus_tag="O0305_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0274966.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            5896..7050
                     /locus_tag="O0305_001826"
     CDS             5896..7050
                     /locus_tag="O0305_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0274967.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            7073..8071
                     /locus_tag="O0305_001827"
     CDS             7073..8071
                     /locus_tag="O0305_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCY0274968.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNFIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(8161..8367)
                     /gene="copZ"
                     /locus_tag="O0305_001828"
     CDS             complement(8161..8367)
                     /gene="copZ"
                     /locus_tag="O0305_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCY0274969.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
BASE COUNT         2868 a         1244 c         1696 g         2581 t
ORIGIN      
        1 aaatatagca aatttaggta caagaattcc tgataagtat tttcgtcaga agtacataat
       61 atttaaatca tttaactttg agaagcatgg aaaattttgg aacaaatggt tttacgtaag
      121 aaaatggaaa cataagattt tagatggtca tcagcttaat caaaatatat atgatcagcg
      181 tcatttaatg acaatcaata ctgatgaaat tgaaaaaatg attatagaga caaagagggc
      241 agagttgatt cattggatat cgatacttcc agtcatcata ttcaataaag gccctcgttt
      301 agtaaagtat ataaatattt tctatgcaat gatagctaat gttccaatca ttattgtgca
      361 acgctataat cgaccgagat taacgcagtt actacgcata ttaaaacgaa gaggtgaacg
      421 tcatgactaa acatatcatc gttattggtg gtggcttagg tgggatttct gcagcaattc
      481 gaatggcaca aagtggctat tcggtctcat tatatgaaca aaataatcat ataggaggca
      541 aagtgaatcg tcatgaatca gatggctttg gctttgattt aggtccatct attttaacga
      601 tgccttatat ttttgaaaaa ttattcgaat atagcaagaa gcaaatgtca gactacgtta
      661 caatcaagcg attgccacat caatggcgta gcttttttcc agatggaacg actatcgatt
      721 tgtatgaagg tattaaagaa acaggtcagc ataatgcgat attgtcgaaa caggatatag
      781 aggaactgca aaattatttg aattatacaa gacgaatcga tcgtattact gaaaaagggt
      841 atttcaacta tggtttagat acactatctc aaattattaa atttcatggg ccattaaatg
      901 ctcttattaa ttatgattat gtacatacta tgcaacaggc catagacaag cgtatctcga
      961 atccatactt gcgacaaatg ttaggctatt ttatcaaata tgtaggttct tcatcatacg
     1021 atgcgccagc tgtattatct atgttattcc atatgcaaca agagcaaggc ctttggtatg
     1081 tagaaggtgg aatccatcat ttagccaatg ccttggaaaa gctagcgcgt gaagaaggtg
     1141 tcacaattca tacaggtgca cgtgtggaca atattaaaac atatcaaaga cgtgtgacgg
     1201 gtgtcagatt agatacaggt gagtttgtaa aggcagatta tattatttca aatatggaag
     1261 tcatacctac ttataaatat ttaattcacc ttgatactca acgattaaac aaattagaga
     1321 gggaatttga gccggcaagc tcaggatatg tgatgcattt aggtgttgct tgccaatacc
     1381 cgcaattagc acatcataat ttctttttta cggaaaatgc ttatctcaat tatcaacaag
     1441 tttttcatga aaaggtattg ccagatgatc cgaccattta tctagtaaat acgaataaaa
     1501 ctgatcacac acaagcgcca gtaggttatg aaaatatcaa agtcttacca catattccat
     1561 atattcaaga tcagcctttt accactgaag attatgcgaa gtttagggat aaaattttgg
     1621 ataaattaga aaaaatggga cttactgatt taagaaaaca cattatttat gaagatgttt
     1681 ggacaccgga ggatattgaa aaaaattatc gttctaatcg tggtgcaata tatggtgttg
     1741 tagcagataa aaagaaaaac aaaggattta aatttcctaa agaaagtcag tattttgaaa
     1801 acttgtactt tgtaggtgga tcagtaaatc ctggtggtgg catgccaatg gttacattaa
     1861 gtgggcaaca agtcgcagac aaaataaacg cgcgagaagc gaagaatagg aagtgatatc
     1921 tatgaaatgg ttatcacgaa tattaacagt aatagtgacc atgtctatgg cgtgtggtgc
     1981 attgatattt aatcgtagac atcagctaaa ggcgaaaacg ctgaacttca atcataaagc
     2041 attaacaatt attattccgg ctagaaacga agaaaaaaga ataggtcatt tactacattc
     2101 gataatacaa cagcaagttc cagtagatgt cattgttatg aatgacggat cgacagatga
     2161 aacagcacgt gtagcacgtt catatggtgc gactgtagta gatgttgttg atgatactga
     2221 cggcaaatgg tatgggaaat cacatgcttg ttatcaaggt gtgacgcatg catgtacgaa
     2281 tcgcattgcc tttgtagatg ctgatgtaac tttcttaagg aaagatgctg ttgaaacgtt
     2341 gattaatcag tatcaattac aaggtgaaaa aggattgtta agcgtacagc cttatcatat
     2401 aacaaagcgt ttctacgaag ggttttcagc gatatttaat ttaatgacag tcgttggtat
     2461 gaatgtattt tctaccttag acgacggtcg gactaaccag catgcatttg gaccggtgac
     2521 attaacaaat aaagaagatt attatgcaac tggaggtcat aaaagtgcaa accgtcatat
     2581 tattgaagga tttgctttag gaagtgcata tacttcacaa tcattgcccg taacagttta
     2641 tgaagggttt ccatttgttg catttcgcat gtatcaagaa ggatttcagt cattacaaga
     2701 aggatggaca aagcatttgt caactggggc aggtggcaca aagcctaaga tcatgacagc
     2761 aattgtgttg tggttgtttg gttctatagc gagtatttta gggctatgtc ttagtttaaa
     2821 atatcgccaa atgtctgtaa gaaaaatggt agcactttac ttgagctata ctacacaatt
     2881 tatttatctg catcgaaggg tcggccaatt ttctaattta ttaatggtat gtcatccatt
     2941 gttatttatg ttttttacta aaattttcat ccaatcttgg aaacaaacgc atcgttatgg
     3001 tgtagttgaa tggaaaggtc gtcaatattc tatatctaaa gaacaataaa tcaaggtaat
     3061 ggcatttcaa tataggagga ctagtatgac aatgatggat atgaatttta aatattgtca
     3121 taaaatcatg aagaaacatt caaaaagctt ttcttacgct tttgacttgt taccagaaga
     3181 tcaaagaaaa gcggtttggg caatttatgc tgtgtgtcgt aaaattgatg acagtataga
     3241 tgtttatggc gatattcaat ttttaaatca aataaaagaa gatatacaat ctattgaaaa
     3301 atacccatat gaacatcatc actttcaaag tgatcgtaga atcatgatgg cgcttcagca
     3361 tgttgcacaa cataaaaata tcgcctttca atctttttat aatctcattg atactgtata
     3421 taaagatcaa cattttacaa tgtttgaaac ggacgctgaa ttattcggat attgttatgg
     3481 tgttgctggt acagtaggtg aagtattgac gccgatttta agtgatcatg aaacacatca
     3541 gacatacgat gtcgcaagaa gacttggtga atcgttgcaa ttgattaata tattaagaga
     3601 tgtcggtgaa gattttgaca atgaacggat atattttagt aagcaacgat taaagcaata
     3661 tgaagttgat attgctgaag tgtaccaaaa tggtgttaat aatcattata ttgacttatg
     3721 ggaatattat gcagctatcg cagaaaaaga ttttcaagat gttatggatc aaatcaaagt
     3781 atttagtatt gaagcacaac caatcataga attagcagca cgtatatata ttgaaatact
     3841 ggacgaagtg agacaggcta actatacatt acatgaacgt gtttttgtgg ataagaggaa
     3901 aaaggcaaag ttgtttcatg aaataaatag taaatatcat agaatatagg tggttgaata
     3961 atgaagattg cagtaattgg tgcaggtgtc acaggattag cagcggcagc ccgtattgct
     4021 tctcaaggtc atgaagtgac gatatttgaa aaaaataata atgtaggcgg gcgtatgaat
     4081 caattaaaga aagacggctt tacatttgat atgggtccca caattgtcat gatgccagat
     4141 gtttataaag atgtttttac agcgtgtggt aaaaattatg aagattatat tgaattgaga
     4201 caattacgtt atatttacga tgtgtatttt gaccacgatg atcgtataac ggtgcctaca
     4261 gatttagctg aattacagca aatgctagaa agtatagaac ctggttcaac gcatggtttt
     4321 atgtcctttt taacggatgt ttataaaaaa tatgaaattg cacgtcgcta tttcttagaa
     4381 agaacgtatc gcaaaccgag tgacttttat aatatgacgt cacttgtgca aggtgctaag
     4441 ttaaaaacgt taaatcatgc agatcagcta attgaacatt atattgataa cgaaaagata
     4501 caaaagcttt tagcgtttca aacgttatac ataggaattg atccaaaacg aggcccgtca
     4561 ctatattcaa ttattcctat gattgaaatg atgtttggtg tgcattttat taaaggcggt
     4621 atgtatggca tggctcaagg gctagcgcaa ttaaataaag acttaggcgt taatattgaa
     4681 ctaaatgctg aaattgagca aattattatt gatcctaaat tcaaacgggc cgatgcgata
     4741 aaagtgaatg gtgacataag aaaatttgat aaaattttat gtacggctga tttccctagt
     4801 gttgcggaat cattaatgcc agattttgca cctattaaaa agtatccacc acataaaatt
     4861 gcagacttag attactcttg ttcagcattt ttaatgtata tcggtataga tattgatgtg
     4921 acagatcaag tgagacttca taatgttatt ttttcagatg actttagagg caatattgaa
     4981 gaaatatttg agggacgttt atcatatgat ccttctattt atgtgtatgt accagcggtc
     5041 gctgataaat cacttgcgcc agaaggcaaa actggtattt atgtgctaat gccgacgccg
     5101 gaacttaaaa caggtagcgg aatcgattgg tcagatgaag ctttgacgca acaaataaag
     5161 gaaattattt atcgtaaatt agcaacgatt gaagtatttg aagatataaa atcgcatatt
     5221 gtttcagaaa caatctttac gccaaatgat tttgagcaaa cgtatcatgc gaaatttggt
     5281 tcggcattcg gtttaatgcc aactttagcg caaagtaatt attatcgtcc acaaaatgta
     5341 tcgcgagatt ataaagattt atattttgca ggtgcaagta cgcatccagg tgcaggcgtt
     5401 cctattgtct taacgagtgc gaaaataact gtagatgaaa tgattaaaga tattgagcgg
     5461 ggcgtataag ggagtagtct aagagaaaga tgtgagaaaa gtataagggg aacgtaaaag
     5521 gcatagtgaa ataagtatgt gcttaagaag ttttatttaa caatgaatga cgcctgttaa
     5581 ggttcacgtt tgaatacgta ccttgatggg cgttatactt tatataagca tatctaactg
     5641 gttgcgcatg cggatatgca tgacagttaa atcataacga ttgagtgcat attaatctga
     5701 aaattcctaa tattgacaat aatattatac ctacctaaaa tgaaaataaa ttcattcaat
     5761 acaacgtgtg tcataagttc tgttgaacat aacggtgaat ggcgatgtca ttgtaaccat
     5821 ctaaatgaga gtgatacaag ttgttaggat tatgatagtc gcccaggcta tgtttaatga
     5881 tagaggagga atttgatgat ctctaacaaa ttagcaaata tccctgatag ttatttcggg
     5941 aaaacgatgg gacgtaaaat cgaacatgga ccattgccat taattaatat ggcagttgga
     6001 atccctgatg ggcccacgcc acaaggtatt attgatcatt ttcaaaaagc actaactatt
     6061 cctgaaaatc aaaaatatgg tgcttttcat ggtaaagaag cgttcaagca agctattgta
     6121 gatttttatc aaagacaata taatgtgacg ttagataaag aagatgaagt atgcatttta
     6181 tatggtacaa aaaatggatt agtggcagta ccaacatgtg ttatcaatcc aggagactat
     6241 gtattactac ctgatccagg ctacacggat tatttagcag gtgtactttt agctgatggc
     6301 aagccagtcc cgcttaattt agaaccgcca cattatttgc cagattggtc caaagttgat
     6361 tcacaaataa tagataaaac aaaactcatt tatttaacgt atccaaataa tccaactgga
     6421 tcgacagcta cgaaagaagt ttttgatgaa gcgatagcta aatttaaagg tacagacact
     6481 aaaatcgtgc atgattttgc ttatggtgct ttcggtttcg acgctaaaaa tcctagtata
     6541 cttgcttcgg aaaatgggaa agatgttgcg attgaaattt attccttgtc taaaggttat
     6601 aacatgtcag gttttagagt cggttttgca gttggtaata aagatatgat tcaagctttg
     6661 aaaaagtacc aaacgcacac aaatgctggg atgtttggtg cattacaaga tgccgcaata
     6721 tatgctttga atcattatga tgatttttta gaagaacaaa gcaatgtatt taaaacacgt
     6781 cgtgaccgat tcgaagccat gcttgcaaaa gctgatttgc catttgttca tgctaaaggt
     6841 ggcatatatg tatggttgga aacaccgcct ggttatgaca gcgagcaatt tgagcaattt
     6901 ttagtgcaag agaagtcaat acttgttgct ccagggaaac catttggaga gaatggtaat
     6961 cgttatgtga gaatttcatt ggcgttagat gaccagaaat tagacgaagc tgcaataaga
     7021 ttaacagaac tagcatattt atatgaataa taaagttaag ggtgatgtta aaatgacaaa
     7081 aattaaaatc atgagtgtac gtgatgaaga tatgccttat atcaaggcgt gggcagagaa
     7141 acatcatgtt gaggtagata ttactaaaga agcattaact gatgacaatg ttgaaggtgt
     7201 cgctggttat gatggcttat cgctatcaca acaaatacca ttatcagagc atgtttataa
     7261 acgattaaat gaactaggta ttaaacaaat cgcacaacgt agtgctggat ttgatacata
     7321 tgatttggag ttagcaaaca agtataactt tattgtgtca aatgtgccgt catactctcc
     7381 gaattcaatt gcggagtttg ctgtgaacca ggctattaat gtggttcgtc attttaatca
     7441 aattcaaacg aaagtgcggg agcatgattt tagatgggaa cctactattt tatcgaaatc
     7501 cattaaagat ttaaaagtag ctgttattgg tacaggtcga attggccgtg tagtagccga
     7561 tatatttgcc aatggttatc aaagtgatgt ggtcgcatac gacccgtttc ctaatgctaa
     7621 aattgcaacg tatgtcgatt acaaagatac gattgaggaa gcggttgaag gtgctgatat
     7681 cgtgacatta catgtacctg caacgaaata taatcattat ctatttaatg ctgaattatt
     7741 taaacatttt aaaaagggcg cagtatttgt caattgtgcg agaggttctt tagtagatac
     7801 taaggcgtta ttagacgcat tagacaatgg tgtgattaaa ggtgcagcac ttgatacgta
     7861 tgaatttgaa cgcaaacttt tcccaagtga tcaaaggggc aaaacactga acgatccatt
     7921 gttagaatcg ttgattgaca gggaagatgt catattaaca ccacatattg cgttttatac
     7981 tgaagctgca gttaaaaatc taattgtcga tgcattagat gcaacattag atgtattgca
     8041 gactggagat actaggttac gagtaaatta aaaatcgaac tgatgagata atttggattg
     8101 ttgggattct catcagttcg attttttaat ttggtgttga tgacgttgaa tattgcctaa
     8161 ttaaacgaca tcgtaacctt gatcttcaat tgcgtctttc atttgagata cagcaacttt
     8221 actgtcatca tattgaacac ttacttgacc attttcaagg ttaacgtcag ctgaagtgac
     8281 accgtcaata ttatttaatg cagattcaac agcacttttg cagtgaccac agctcatacc
     8341 ttcaacattt aaaatttctt gtgacatagc attcacctcc ttttagagc
//
LOCUS       DAKTIP010000112         8359 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAKTIP010000112 DAKTIP010000000
VERSION     DAKTIP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.112"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..143
                     /locus_tag="O0305_001829"
     CDS             <1..143
                     /locus_tag="O0305_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0274970.1"
                     /translation="IKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHF
                     AA"
     gene            157..828
                     /gene="modB"
                     /locus_tag="O0305_001830"
     CDS             157..828
                     /gene="modB"
                     /locus_tag="O0305_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCY0274971.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            829..1434
                     /locus_tag="O0305_001831"
     CDS             829..1434
                     /locus_tag="O0305_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0274972.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            1604..2608
                     /locus_tag="O0305_001832"
     CDS             1604..2608
                     /locus_tag="O0305_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCY0274973.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            2639..3145
                     /locus_tag="O0305_001833"
     CDS             2639..3145
                     /locus_tag="O0305_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCY0274974.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     DGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3147..3641)
                     /gene="moaC"
                     /locus_tag="O0305_001834"
     CDS             complement(3147..3641)
                     /gene="moaC"
                     /locus_tag="O0305_001834"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCY0274975.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTLIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            3704..4963
                     /locus_tag="O0305_001835"
     CDS             3704..4963
                     /locus_tag="O0305_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCY0274976.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETKTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQKDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFVDGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            4960..5445
                     /gene="mobB"
                     /locus_tag="O0305_001836"
     CDS             4960..5445
                     /gene="mobB"
                     /locus_tag="O0305_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCY0274977.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            5459..5905
                     /locus_tag="O0305_001837"
     CDS             5459..5905
                     /locus_tag="O0305_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCY0274978.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            5911..6144
                     /gene="moaD"
                     /locus_tag="O0305_001838"
     CDS             5911..6144
                     /gene="moaD"
                     /locus_tag="O0305_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCY0274979.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6151..6750
                     /gene="mobA"
                     /locus_tag="O0305_001839"
     CDS             6151..6750
                     /gene="mobA"
                     /locus_tag="O0305_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCY0274980.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            6770..7792
                     /gene="moaA"
                     /locus_tag="O0305_001840"
     CDS             6770..7792
                     /gene="moaA"
                     /locus_tag="O0305_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCY0274981.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
     gene            complement(8187..8357)
                     /locus_tag="O0305_001841"
     CDS             complement(8187..8357)
                     /locus_tag="O0305_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274982.1"
                     /translation="MFRFISFGQLVVYSTTFIPKKDILQLNGMECDINVKEPLKIYYV
                     VILKSGIEMIFS"
BASE COUNT         2892 a         1204 c         1701 g         2562 t
ORIGIN      
        1 taattaaaga agtagaactt aagaagccaa tcacatacga agctggtgct acatcagata
       61 gtaaattagc aaaagagtgg atggaattct taaaatcaga taaagctaaa gaaatactaa
      121 aagaatacca ctttgcagca taaggagttg taatccatgc ctgacttaac acctttttgg
      181 atatcaatac gagttgctgt aatcagtacg attattgtaa cggttttagg tatttttata
      241 tctaaatggt tgtatcgtcg taagggttcg tgggttaaat tattggaaag tttattgata
      301 ttacctattg ttttgccgcc aacggtatta ggttttattc tattaatcat cttctcgcca
      361 agaggaccaa tcggtcaatt ctttgcgaat gtactacatt tacctgtagt gttcactttg
      421 acaggtgctg tgatagcatc tgtcattgtt agttttccac taatgtatca acatactgtg
      481 caaggcttca gaggtataga cacgaaaatg attaatacag ctagaacgat gggagcaagt
      541 gaaacgaaaa ttttcctcaa attaatttta ccattagcta aacgctctat tttagcaggt
      601 ataatgatga gttttgctcg tgcattaggt gagtttggtg ctacattaat ggttgcagga
      661 tatattccaa ataaaacgaa tacactacct ttagaaatat acttcttagt ggaacaaggt
      721 agagaaaatg aagcgtggtt atgggtatta gtgctagtcg cattctctat tgtggttata
      781 tctacaatta atttattgaa taaagataaa tataaggagg tcgactagat gcttaaaatc
      841 aatgtgaaat atcaattaaa gaacacttta attcgcatca atatagatga tactgaacca
      901 aaaatttatg cagttcgtgg tccatctggc attggtaaaa ctactgtttt aaatatgatt
      961 gccggattac gtaaagcaga tgaagctatt atcgaagtga atgggcaatt acttactgat
     1021 acggcaaaaa acgtgaatgt taaaattcaa caacgacgta ttggatatct gtttcaagac
     1081 taccaattgt ttcctaatat gacggtctat aaaaatatta cttttatggc tgaaccatct
     1141 gaacacatcg atcaattaat tcaaacttta aacattgatc atttgatgaa acaatatcct
     1201 atgacattgt caggtggaga ggcacaacgt gtagcacttg cacgtgcact tagcacgaaa
     1261 ccagatttaa ttttattaga tgaacctttt tctagtttgg atgatactac aaaagatgag
     1321 agtattacat tagttaaacg tattttcaac gaatggcaaa taccaatcat atttgtgaca
     1381 cattcaaact atgaagcaga acaaatggct catgaaatta ttacaattgg gtaatcattt
     1441 atttgccatt aaagagttta gaacgtattt aaaattgtag aagtgaatgc ttctatcagc
     1501 attttaatga tgttttaaac tcttttttag gggcagtttt tttgagagac attgacgcgc
     1561 gtcatataat gaaagtaatg ataaaaagaa aggataactt aatgtgagtc aagaacgtta
     1621 ttcaaggcaa attttattta aacaaatagg tgaaataggt caaagcaaaa taaatcaaaa
     1681 atgtgcgttg attattggta tgggcgcatt aggtacacat gtggccgaag gacttgttag
     1741 agcaggcatt gccaaactaa tcattgttga tagagattat attgaattta gtaatttaca
     1801 aagacaaaca ttgtttactg aagaagatgc tttgaaaatg atgcctaagg tggttgcagc
     1861 taaaaagcat ttgctagcgt tacgtagtga tgttgatatt gatgattata ttgcccatgt
     1921 ggattattat tttttggaaa cacatggaca ggacgttgac gttattattg atgcaaccga
     1981 taactttgaa acacgacaac tgattaatga ttttgcatat aaatatcgta taccttggat
     2041 ttatggtggt gttgtacaga gtacatatac agaagctgca tttatacctg gtaaaacacc
     2101 ttgctttaac tgtttggtac cacaattgcc agcattaaat ttaacatgtg atacagtagg
     2161 ggtcattcaa cctgccgtga cgatggcaac aagtttacaa ttaagagatg cgatgaaagt
     2221 attaacggaa caaccaattg acacaaaaat aacttatggc gatatttggg aaggtagtca
     2281 ttattcattt ggtttcagta aaatgcaacg ttcagactgt acaacttgtg gagatgtacc
     2341 aagttatccg tatttaaaca agaatgaaca acgttatgca acattgtgtg gtagagacac
     2401 tgtacagtat gaaaatgcat caattacaca cgacattctt gttcaatttt taaaacaaca
     2461 tcagttaaat tatcgcagta attcgtatat ggttatgttt gaatttaaag gacaccgcat
     2521 tgttgctttt aaaggtggaa ggtttttaat acatggcatg acacgcacat cagatgccac
     2581 acatctaatg aatttattgt ttggataaaa aaagataaga caaaaggagt gtaatattat
     2641 gggcgaacat caaaacgtta aattgaatcg tacagttaaa gcagccgtac taacggtatc
     2701 agatactaga gactttgata cagataaagg tggtcaatgc gtgcgccaac tattacaagc
     2761 agatgacgtt gaagtgagtg acgcacatta tacaattgtg aaagatgaaa aagtagccat
     2821 cacgacgcag gtgaagaagt ggttagaaga agatattgat gtcatcatta cgactggtgg
     2881 aacaggtatt gcacaacgtg atgtgacgat tgaagcagta aaaccacttt taactaaaga
     2941 gatagatggc tttggggaat tgtttagata tttgagttat gttgaagatg ttggcacgcg
     3001 tgcattattg tctcgtgctg tagcaggtac agttaataat aaattgatat tttcgattcc
     3061 aggatcaaca ggcgcagtta aattagcatt agaaaagctc attaaaccag aattaaatca
     3121 tctgattcat gagcttacaa aataatttat tgatttgatt ggcgttgaaa atctccagat
     3181 ttaccgccag acttgctttc aaggtaggtt tcgccaataa tcataccttt atcaactgct
     3241 ttcgtcatgt cgtaaatggt taaagccgtt gctgatgcag cggttaaagc ttccatttca
     3301 acaccggttt tgccagttgt agagacagtt gtttgaatgt ttaaagtata aaggggtgca
     3361 tttgtttcat cccagctgaa gtgaacatct atgccagtca atggtaatgg atggcacatc
     3421 ggaataagtg ttgatgtatt tttggcagcc ataataccag cgatttgagc agtgttcaat
     3481 acattgcctt tggtattggt gttattagaa atctgcttaa aaatagtttc atttacagta
     3541 atgcttgaat gagcgacagc agttcttttt gtaatttgtt tgtctgatac atcgaccatt
     3601 ttggcgtggc cttgttgatt aatatgagta aactcagtca ttttacccct cctagtgcat
     3661 ctagtatatc atgaaaaaat aaaagttttg gagatgattt ttaatggtag tagaaaaaag
     3721 aaacccaatc ccagttaaag aagcaattca acgtatcgtt aatcagcaga gttcaatgcc
     3781 ggcaattacg gtagcacttg aaaaaagtct aaatcatatc ttagcagaag atattgtagc
     3841 tacttatgat ataccaaggt ttgataaatc accttatgat ggttttgcaa ttcgcagtgt
     3901 tgattcacaa ggggcaagtg gtcagaatcg cattgagttt aaagtgattg atcatattgg
     3961 tgcaggttca gtttctgata aattagttgg ggatcacgaa gcggtgcgta ttatgactgg
     4021 agcacaaata cctaatggcg cagatgctgt tgttatgttt gaacaaacga ttgaactaga
     4081 agatacattt acaattcgta aaccattttc aaaaaatgaa aatatatctt taaaaggtga
     4141 agaaacaaag acaggcgatg ttgttctaaa aaaaggacaa gtaattaatc caggggctat
     4201 cgcggtcctt gcaacatatg gctatgcaga ggttaaagtt attaagcaac cgagtgtcgc
     4261 tgttattgca acaggaagcg aattattaga tgttaatgat gtattagaag atggaaaaat
     4321 tcgtaactct aatggcccaa tgattcgtgc cttagcagaa aaattaggtc ttgaagttgg
     4381 tatttacaaa acacaaaaag atgatttaga tagtggcatc caagtcgtta aagaagctat
     4441 ggaaaaacat gatatcgtta ttacaacggg cggagtttct gttggagatt ttgactattt
     4501 acctgagatt tataaggctg taaaggcgga agtgttattt aataaagtag caatgcgtcc
     4561 tggtagcgta acaacggttg catttgtaga tggaaagtat ttgtttggat tatctggaaa
     4621 tccatcagct tgttttacag gatttgaact atttgtgaaa ccagctgtta aacatatgtg
     4681 tggcgcacta gaagtcttcc cgcaaataat taaagcaaca ttaatggaag attttaccaa
     4741 ggcaaaccca ttcacacgat ttatacgtgc taaagcaacg ttaacaagtg ctggagctac
     4801 tgtagtacct tcaggattca ataaatcagg tgcggttgta gcgattgcac atgctaactg
     4861 tatggtcatg ttaccaggag ggtcacgtgg ttttaaagcg gggcatacag tagatattat
     4921 attgactgaa tctgacgctg ctgaagagga acttctttta tgattttaca aattgtaggt
     4981 tacaaaaagt ctggtaagac aacattgatg aggcatattg tctctttctt aaagtcacat
     5041 ggttatacag ttgctactat taaacatcat gggcatggta aggaagatat tcaattacag
     5101 gattcagacg tcgatcacat gaagcatttt gaagcggggg cagatcaaag tattgtacaa
     5161 ggttttcaat atcagcaaac tgtaacacgt gtagataatc aaaatcttac tcaaattatt
     5221 gaaaaatctg ttacaattga caccaatatc gtattagttg aaggctttaa aaatgctgat
     5281 tttgaaaaag tcgtagtcta tcgaaatgaa gaagagttgc aagtattaca acaattgtcg
     5341 aatgtttgtt atagcattaa tgtaagggag catgaagatt ttacagcatt tgagcaatgg
     5401 ttattaaata aaattaaaaa tgattgtgat acacaattaa catagaggat tgaaatgaat
     5461 gaaacaattt gaaatcgtga cagaaccgat acaaacagaa caatatcgtg aattcactat
     5521 aaatgaatat caaggtgcag tagttgtttt taccggtcat gttcgcgaat ggactaaagg
     5581 cgtcaaaacg gaatatttag aatatgaagc gtatattcca atggctgaaa agaaattggc
     5641 acaaattgga gatgaaataa atgaaaaatg gcctggaacg ataacgagta ttgttcatag
     5701 aatagggcca ttacaaattt cagatatcgc tgtattaatt gcggtttctt caccgcatcg
     5761 taaagatgcc tatcgagcaa atgaatatgc aattgagcgt ataaaagaaa ttgttccgat
     5821 ttggaaaaaa gaaatttggg aagatggttc aaaatggcaa gggcatcaaa aagggaatta
     5881 tgaagaagcg aagagggagg aataagagag atgaaggtac tttacttcgc agaaattaaa
     5941 gatatattac aaaaagcaca ggaagatatt gtgcttgaac aagcattgac tgtacaacaa
     6001 tttgaagatt tattgtttga acgttatccg caaatcaata ataaaaagtt tcaagttgct
     6061 gtaaatgagg aatttgtaca aaaatcggat ttcattcaac ctaatgatac tgttgcatta
     6121 attccaccgg ttagtggagg ttaagggagc atgaaagcaa taattcttgc aggtggtcat
     6181 tcagtgcgat ttggtaagcc caaagctttt gcggaagtga acggtgagac cttttatagt
     6241 agagtaatta agacattaga atcaacaaat atgttcaatg aaattattat tagtacaaat
     6301 gcgcaattgg caacgcaatt taaatatcca aatgttgtta tagatgatga gaatcataat
     6361 gataaaggtc cattagcagg aatttataca atcatgaagc aacatcctga agaagaattg
     6421 ttttttgtcg tttctgttga tacaccaatg attactggta aagctgtaag cacgttgtat
     6481 cagtttttag tttctcatct tattgaaaat catttagatg tcgcagcttt taaagaagat
     6541 ggacgtttta ttccaacaat tgcattttat agtccgaatg cattaggcgc tataactaaa
     6601 gcactacatt ctgataatta cagttttaaa aatgtatatc atgaattatc aacggattat
     6661 ttggatgtaa gggatgtaga tgcgccctca tattggtaca aaaatataaa ttatcagcat
     6721 gatttggacg ctttaattca aaaattgtaa gctgttagga ggtccacaaa tggtagaaca
     6781 aataaaagat aaactaggac gtcccatccg tgacttacgg ttatctgtga cagatcggtg
     6841 taactttagg tgtgattatt gcatgcctaa agaggtattt ggagatgatt tcgtattttt
     6901 acctaaaaat gaacttttaa cgtttgatga aatggctaga atcgctaagg tatatgcaga
     6961 attaggtgta aaaaaaatac gcattacagg tggagaacca ttgatgcgac gggatttaga
     7021 tgtacttata gctaaattaa atcaaatcga tggtattgaa gatattggtt tgactacaaa
     7081 tggtttgtta ttaaaaaagc atggacaaaa gttatatgat gctgggctgc gcagaattaa
     7141 tgtcagtttg gatgctattg atgatacgct atttcaatca atcaataatc gtaatattaa
     7201 agcgactacg attttagaac aaattgatta cgcgacgtct attggtttga atgtaaaagt
     7261 aaatgttgtt atacaaaaag gtattaacga tgatcaaatc ataccaatgc ttgaatattt
     7321 taaagataaa catatagaga ttcgatttat agaatttatg gatgttggta atgataatgg
     7381 atgggatttc agtaaagttg taactaaaga tgaaatgctt acaatgatag agcagcactt
     7441 tgaaatcgat cctgtagaac caaaatattt tggggaagta gcaaaatatt atcgccataa
     7501 ggataatggt gttcaatttg gtttgattac aagtgtttca caatcatttt gttctacatg
     7561 tacacgcgca aggctgtcat cagatgggaa gttttacgga tgtttatttg caactgtcga
     7621 tggatttaac gttaaagcgt ttattcgttc tggcgtgacc gacgaagaat taaaagaaca
     7681 atttaaagct ttatggcaaa taagagatga tcgatattca gatgagagaa ctgctcaaac
     7741 agttgccaat cgtcaacgta aaaagataaa catgaattat attggtggtt aatgtgtagg
     7801 gaccactaca tattaaatca ttagagatgt tttaatattt ctgtcttact ccctaaaata
     7861 caatattatt tattaaagta aaaacggtca tatctatgcc agatttaata gaaatgatcg
     7921 tttttaaagt ttttacaagt tggcggggcc ccaacacaga agctgacaga aagtcagctt
     7981 acaataatgt gcaagttggc ggggccccaa catagagaat ttcaaaaaga aattctacag
     8041 acaatgcaag ttggggaacg gggccccaac acagaaggtg acgaaaagtc agcatacaat
     8101 aatgtgcaag ttggcggggc cccaacatag agaatttcaa aagaaattct acagacaatg
     8161 caagttgggg atcaacgaaa taaattttat gagaatatca tttctatccc actcttaaga
     8221 atcactacat aataaatctt tagtggttct ttaacattga tgtcacactc catgccattg
     8281 agttgtaata tatctttttt aggtataaat gttgtcgaat aaacaacaag ttgtccaaaa
     8341 gatataaatc taaacaaga
//
LOCUS       DAKTIP010000113         8350 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAKTIP010000113 DAKTIP010000000
VERSION     DAKTIP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.113"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..156)
                     /locus_tag="O0305_001842"
     CDS             complement(<1..156)
                     /locus_tag="O0305_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274983.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTY"
     gene            complement(316..1194)
                     /gene="hchA"
                     /locus_tag="O0305_001843"
     CDS             complement(316..1194)
                     /gene="hchA"
                     /locus_tag="O0305_001843"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCY0274984.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            complement(1460..2647)
                     /locus_tag="O0305_001844"
     CDS             complement(1460..2647)
                     /locus_tag="O0305_001844"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCY0274985.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            2818..3993
                     /locus_tag="O0305_001845"
     CDS             2818..3993
                     /locus_tag="O0305_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCY0274986.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGKEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTFKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            complement(4275..5459)
                     /gene="tuf"
                     /locus_tag="O0305_001846"
     CDS             complement(4275..5459)
                     /gene="tuf"
                     /locus_tag="O0305_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCY0274987.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(5676..7757)
                     /gene="fusA"
                     /locus_tag="O0305_001847"
     CDS             complement(5676..7757)
                     /gene="fusA"
                     /locus_tag="O0305_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCY0274988.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            complement(7880..8350)
                     /gene="rpsG"
                     /locus_tag="O0305_001848"
     CDS             complement(7880..8350)
                     /gene="rpsG"
                     /locus_tag="O0305_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCY0274989.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
BASE COUNT         2562 a         1599 c         1374 g         2815 t
ORIGIN      
        1 atatgtatgt ggtattttca aaccatttaa ttcactctca atgacaccat gtgtatatgg
       61 tttcacaaat tttgatacta cttgaccgtt agttgtatta attaaaaaca cacggcctga
      121 agcagttcca taatcaattc caatgctata agacatcatg tttcctccat atcgctagtt
      181 gcggttatta gttttgcaag cgttttctta aatcatactc ataataggtt ctattttaca
      241 agtatcactt aaattttgcc aaaaaaaaag agccctcata catttacata tgaggctcct
      301 ctaattaata tttaattatt tattttgtat tgcatttaac atttcattaa ctgctaattt
      361 tcctaactca tttgaagcta aaggactgtc acctgttaat aatttacgat cttttaacgt
      421 tcttcctgtc atatcgtcgt taactacttt taatccttgt ttagttaata aatcagcaac
      481 taaccatttc aagcgtccag gtaaataacc tatttcaata tttgcacctt catctaatga
      541 gtcagggaag acacaaacag agtatccttc taatggagat ttttctctgt taagccctgc
      601 tgaaagtagt gctgctggtc catgacataa tgtaactata aagcggtcat tgtctaatgc
      661 ccaatctaat gtttgttgaa cgtcctcact ttcagaaata ccaacaactg cagcatgtcc
      721 gcctgggata aagacagata aatagtctga atcaggtcct aattcatttt taatcacatc
      781 tgctaatttt tttggctgtt ttaatttttc tttcaattta ttataagtac ttataactgc
      841 ctcgtcttca gttggcatag cccataattc taatttaact ggataaccag ataatgtcgc
      901 aacatcaacg tcaaaacctg cttccattaa atgatgtaaa ggtaataaca tttcaacagg
      961 atgattaccc gtagagaaca tttttccatt ttccaataat acatatcgct cttcagcagc
     1021 aatcatcaat actttccatt taccatcttt ataggcacct ttgtgttcaa caccatcaaa
     1081 atctgtttta ggtgctgtat attgactaag ggaatatggt gatgggaaaa atgcgttatc
     1141 ttctgcttta tctggcgttg gttgcttact taattcattt acatcttgtg acatagttta
     1201 ttcctacttt ctctatatag ttactactag tatagacata tactacacac tataaacatg
     1261 tattactatt aaaatgctca ttatcaaaaa taatcaaaat gaatttcttt gaaaaaaata
     1321 caactaaatt ctacaagatg cttttaaaat tcaaacttca attttgggga gcactaaaca
     1381 ttgatatacg aacttactgt taataaacta aacattaaaa aaagcccacg acaatatttg
     1441 ccgtgggaat aaataaatat taaatcaact tcatttcttt tcctactttt tcataagccg
     1501 caattgcttc atctaacatg tcttttgtat gtgcagctgt aggcatattt cttacacgtc
     1561 ctgtacctct tggtactgtt gggaaaacga tagatttcac atagacacct tcgtctttta
     1621 aacgcttact aaattcttga gttgtttttt catcaccaat aattactggt gtaattggag
     1681 tttctgactc acctgtatca tatcctaatt ttgacaatcc attttttaaa tattgtgcat
     1741 tgttccataa tttatcatgt aattcagttg aatccattaa ctttttaact gcttcagtta
     1801 ttgctttggt atccccaggt gctaatgatg tagagaataa gaatggtcgt gattgtgctt
     1861 ttaaccaatc tattaactct tttgtacctg ctacataacc gccaacgaca ccaattgctt
     1921 tagaaagcgt acctatttgg aaatcgattt tatcttgtaa accaaaatgt ttaaccgtac
     1981 cagcgccttt acccataaca cctgaaccat gagcgtcgtc aacataagtt aataaaccaa
     2041 attcttctgc aatttctaca atttcaggta atttagccac atcaccatcc atactaaaaa
     2101 cgccatcagt gatatacatc actttattgt attgacctga ttcaactgct tcttttgctt
     2161 tcgcacgtaa atcatccatg tctgaatggt taactcgaat aattttagct ttagataagc
     2221 gacatccatc aataattgat gcatgattaa gctcatctga taaaatagca tcatttttat
     2281 tcatgacagc tgaaatagca gccatattac aattaaatcc tgattgataa gctattgcag
     2341 cttctgttcc tttaaatttt gctagtgttt cttctaattc gtcgtgtaaa tctaatgtac
     2401 cattgattgt acgaacagcg cctgcaccta caccatgtgt atcaatagct gcttttgcag
     2461 ctgatttcaa atcttcattt gttgctagtc ctaaataatt atttgaagat aagttaatgt
     2521 atgatttccc attgattttg atttctggtc cgtttgcacc ttcaattgta tctatttcat
     2581 tatacaaacc attttctttt agataattta tattttcctc taaaaactca tgtaatgatt
     2641 gaaccacagt atataatccc ccttaagtat tgtttggtaa tacctttatc atacagtaat
     2701 tctacataac ttgaaagcct tatcattcat agataaaata tctcaaagca atcatttagt
     2761 acgataattt cataaaatgt tatactataa acattatttt agaaagtagt gataagggtg
     2821 ttagattggt tccaactagc aaataataaa gaaaataaaa caattcaact cagacgttat
     2881 ttacaccaat atcctgagtt atcttttgaa gaatttcaaa cacatgatta tattgttaac
     2941 caattaagcc agttatcttg tgatattgaa actcctatcg gccgcaatgg tatcaaagcc
     3001 acatttaaag gcttgggcac aggacctacc attgcattaa gagctgactt tgatgcgtta
     3061 cctgtcgagg aattaaacga tgtgccttat aagtctaaaa atccaggatg tatgcatgcg
     3121 tgtgggcatg atggtcatac agcaatttta cttactgtag cagagatttt agatgaacat
     3181 aaacatttat tagaaggtaa tgttgtatta atattccagt acggtaaaga aatcatgcca
     3241 ggtggttctc aagaaatgat agatgctgga tgtctggaaa atgtagatag aatttacggt
     3301 acacatttat ggagtggtta tcctactgga actattcatt cacgtgcggg ggcaatcatg
     3361 gcctctccag atgaatttag cgtcacattt aaaggtcgtg gtggtcatgg tgccaaacct
     3421 catgaaacca ttgacccaat agtaatcatg gcagagttca ttttaagtgc acaaaaaata
     3481 atttcacgta cgatcgatcc agttaaacaa gctgtacttt catttggaat gattcaagca
     3541 ggcaccacag atagcgttat tcctgatcaa gcattttgta aaggtacagt tagaactttc
     3601 gattcagata tacagaatca tgtaatggac aaaatggata agcttctaca aggtttagca
     3661 atagcaaatg atattaacta tgatttgaat tatattaaag ggtatttgcc tgtccataat
     3721 aacgagaaag catatcaagt tattaaagaa gcaacaaatg acttacatgt acgctttaat
     3781 gaatcagatt taatgatgat tggagaagat ttttcacatt atttgaaagt acgaccaggt
     3841 gcattctttc tgactggttg tggtaatgaa agtaaaggta ttacagcgcc acaccataat
     3901 cccaaatttg atattgatga aaaatcatta aaatacgctg tagctgtatt tttaaaaatc
     3961 atagaattag agcaagtatt taaaacaaat taagcattta aattaattga aagatatgaa
     4021 tctgagacat aaactaatga aatgctccct tcaaagtaga cattgaaaaa atgaaaactt
     4081 tgaagggagc attttacaat gtccaagact caacaatttt atttaaacag tattatcagt
     4141 tataagtttt attagtcagt ctttcatcaa ttattgttct tctaaatcga atagccatta
     4201 caaaatttac acattaaaaa aagacccctc gattgaggga tcttcttttt atataaaatc
     4261 taagaaatta gaaattattt aatgatttca gtaacaacgc ctgatcctac agtacgtcca
     4321 ccttcacgga ttgagaaacg agtaccgtct tcaatcgcga ttggagcgat taattctact
     4381 gtcatttcaa cgttatcacc aggcattacc atttcagtac cttctggtaa gtgaacaaca
     4441 ccagttacgt cagtagtacg gaaatagaat tgtggacgat agtttgagaa gaatggagtg
     4501 tgacgtccac cttcgtcttt tgataatacg tatacttctg ctttgaattc agtatgtggt
     4561 gtaattgaac caggagcagc taatacttga ccacgttgta cgtcttcacg agcaacacca
     4621 cgtaataatg caccaatgtt gtcaccagct tcagcgtagt ctaataattt acggaacatt
     4681 tcaacacctg taacagttgt tttagatgtg tcatgtaaac cgatgatttc aacttcttca
     4741 ccaactttga tttgaccacg ttcaacacgg cctgtagcaa cagtaccacg accagtgatt
     4801 gagaatacgt cctcaactgg catcatgaat ggtttgtcag aatcacgttc tggagttgga
     4861 atgtaagtat ctacagcttc cattaattct aagatttttt cttcgtattg agcatcgcct
     4921 tctaaagctt ttaatgctga accagcgatt acaggtacat cgtcacctgg gaagtcatat
     4981 tcgcttaata agtcacgaac ttccatttct actaattcta ataattcttc atcgtcaacc
     5041 atgtcaactt tgtttaagaa tactactaat gctggtacac caacgttacg tgataaaaga
     5101 atgtgttcac gagtttgtgg cattggaccg tcagcagcag atactactaa gataccgccg
     5161 tccatttgag cagcaccagt gatcatgttt ttaacgtagt cagcgtgtcc tgggcagtca
     5221 acgtgagcgt agtgacgttt gtcagtttgg tactcaatgt gagaagtatt gattgtgata
     5281 ccacgttctt tttcttctgg agcgttgtca atcatgtcat atgattgtgc aactgagtca
     5341 ccattttttg ctaatacagt agcgattgct gctgttaatg ttgttttacc atggtcaacg
     5401 tgaccgatag taccgatatt ggcatgttct ttagaacgat cgaatttttc ttttgccatt
     5461 ataaaatctc tcctattcta attaaaaata atatttaaat ttatctctca tgatagtttc
     5521 tcaccatcat gagaaattat ttatagcgat gcaaaaagaa ttttacaatt cttttataat
     5581 gcattcaccc caaaaaatca aactataaag atgatagctt acatttaagc tcaccttgta
     5641 ttttaaccta ggctagctag tcaaaacaag ttatattatt cacctttatt tttcttgata
     5701 atatcttcag cgattgattt tggaacttca gcatagtgat cgaagtacat agtgtaagta
     5761 ccgcgacctt gagtgtttga acgtaatgat gttgcataac cgaacatttc tgaaagtggt
     5821 acataagcat taacaacttg tgcattacca cgaggttcca taccatcaac acgtccacga
     5881 cgagatgtta cgtcacccat gatatcaccc atgtactctt caggcatttc aatagttact
     5941 ttcatcattg gttctaagat tacaggatca cattttttag cagcttcttt aagtgctaat
     6001 gatgcagcaa ttttgaaggc catttcagat gaatcgacat catggtatga accatcatat
     6061 aatttagctt taacatcaat taaaggataa cctgctaaaa caccattttc catagcatct
     6121 ttaagaccag cttctactga tggaatgtat tcacgaggaa ctacaccacc aacgatagcg
     6181 ttttcgaatt cgaaacctgc gcctgtttcg tttggtgtga attcaatgtg aacatcaccg
     6241 tattgaccac gaccaccaga ttgacgagag aatttacctt gaacttgtgc agatgatttg
     6301 aatgtttcac gatatgaaac cattggagca cctacgttac attcaacgtt gaattctttc
     6361 ttcatacggt ctactaagat gtctaagtga agctcaccca taccaccgat gataacttgt
     6421 ccagtttctt cgtcagtgtg tgcatggaat gttgggtctt cttcttgtaa tttaactaaa
     6481 gcttgagtca ttttatcttg gtcagcttta gattttggct ctactgataa gtgaataact
     6541 ggctctggga attccattga ttccaagata atgtcatttt tctcaccaca taaagtatca
     6601 ccagtacctg tatctttaag acctaccgca gcagcgatat ctccagagta tacagtatcg
     6661 atttcttgac gtgagttagc gtgcatttgt aataaacgac ctacacgttc acgtttacct
     6721 ttagtagagt tcttaacgta tgaaccagat gtcattgtac ctgaatacac acggaagaat
     6781 gttaatttac caacataagg gtcagtcata actttgaacg ctaatgcagc gaattcagct
     6841 gaatcgtctg ctttcgcgat tacttcttct tcagggttgc tagcacggtg accaataatt
     6901 ggtttaacgt ctagtggtga aggtaagtaa tcaattacag cgtcaagcat taattgaaca
     6961 cctttgtttt tgaaagctgt accacaaagt actgggtaga attctacgtt agtagtagct
     7021 tggcggatag cttcttttaa ttcagaaact gaaatttctt cgtcaccaag atatttttcc
     7081 attaattcgt cgctagtttc tgcaactgct tcgattaagc tagcacgagc ttcttcagct
     7141 ctatctaagt ggtcttcggg aatttcaatt tcttcaattt cagtacctaa atcatttgta
     7201 tatttgaaac atttcatttc aactaagtca atgattgctt cgaattcgtc ttccgcacca
     7261 attggtaatt ggattggagc agcgttagct tgtaaacgat catgtaatgt acttacagag
     7321 tattcgaagt tagcacctaa tttgtccatt ttgtttacaa atacgatacg tggaacacca
     7381 taagttgtag cctgacgcca aactgtttca gtttgaggtt caacacctga ttgtgcatca
     7441 agtactgtaa ctgctccgtc aagtacacgt aatgaacgtt caacttctac agtgaagtct
     7501 acgtgtccag gtgtatcgat aatgtttaca cggtgacctt cccaagctgc tgttgttgca
     7561 gcagatgtga tagtaatacc acggtcttgt tcttgctcca tccagtccat ttgtgaagca
     7621 ccttcgtgtg tttcaccaat tttgtggata cggccagtgt aataaagaat acgttcagtc
     7681 gtagtcgttt taccagcatc aatgtgagcc atgataccga tattacgagt tttttctaat
     7741 gaaaattctc tagccatgta ttttttctcc ttccagttta tattactgaa taaatacgat
     7801 agatatggcg atgccctaag catgaacgct taatgaaaat tcattaatat agaacacact
     7861 cagggtaaaa gcttttatct taccaacggt agtgagcaaa tgctttgttt gcttcagcca
     7921 ttttgtgagt gtcctcacgt ttcttaacgg caccacctgt attatttgct gcatctaaaa
     7981 tttcgttagc taaacgatct tccatcgttt tttcaccacg aagacgtgca tagttaacta
     8041 accaacgtaa acctaaagta gtacgacgct ctggacgaac ttctactggt acttgatagt
     8101 tagaaccacc tacgcgacga gctttaactt ctaatactgg cataatgttg ttgattgctt
     8161 cttcgaatac ttctaatgca tcacgaccac tgcgttgttc aactaggtcg aatgctgaat
     8221 aaagaattct ttgtgctgtt ccacgtttac catctaacat aattttgtta attaatttag
     8281 ttactaactt agagttatga attggatctg gtaatacgtc tcttttaggt actgatcctt
     8341 tacgaggcat
//
LOCUS       DAKTIP010000114         8333 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.114, whole genome shotgun sequence.
ACCESSION   DAKTIP010000114 DAKTIP010000000
VERSION     DAKTIP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.114"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            201..1073
                     /locus_tag="O0305_001849"
     CDS             201..1073
                     /locus_tag="O0305_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCY0274990.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
     gene            complement(1460..2839)
                     /locus_tag="O0305_001850"
     CDS             complement(1460..2839)
                     /locus_tag="O0305_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0274991.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EELEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            3119..3475
                     /locus_tag="O0305_001851"
     CDS             3119..3475
                     /locus_tag="O0305_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274992.1"
                     /translation="MLKQILLRATKISVLFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTVFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            3472..3657
                     /locus_tag="O0305_001852"
     CDS             3472..3657
                     /locus_tag="O0305_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274993.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(3753..3944)
                     /locus_tag="O0305_001853"
     CDS             complement(3753..3944)
                     /locus_tag="O0305_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274994.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(3955..4590)
                     /locus_tag="O0305_001854"
     CDS             complement(3955..4590)
                     /locus_tag="O0305_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0274995.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(4665..5582)
                     /locus_tag="O0305_001855"
     CDS             complement(4665..5582)
                     /locus_tag="O0305_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCY0274996.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFVSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            5850..6380
                     /locus_tag="O0305_001856"
     CDS             5850..6380
                     /locus_tag="O0305_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0274997.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(6446..8050)
                     /locus_tag="O0305_001857"
     CDS             complement(6446..8050)
                     /locus_tag="O0305_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCY0274998.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASSGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
BASE COUNT         2955 a         1547 c         1138 g         2693 t
ORIGIN      
        1 tattatttgc acatgggagt atgctatttt ttatctatac atcttaaaaa aatttacagt
       61 tttacaattc aatatcatta aacaaacaac atattcagct tatttttcat tctcttacgt
      121 tacaagttgt aaataccatc gcgttaaaca acaatagcca acgctaataa aatatattgc
      181 gttaaagtgg agtgattaat atgtcaaacg tactaacaga aatagatagt caatatccat
      241 atatgactaa aaatgaaaaa aagatagcta aatttatact aaattcacct caaaaggtga
      301 ttaaaatgag gtctcaagac ttagcaagct tattagatat tagtacatca tcagtcattc
      361 gattcagcaa gaaaattact gatggcggtt ttcacgactt gaaaataaat atatctaaat
      421 atgttcctaa agcatcatca atttacaatg ttgaattaat gaataacgaa agcacagaat
      481 ctttaagaac taaactccat acacgtacta cacgcgcact taatcatgca aataacgaat
      541 taaatgataa aactattgat caaatatgtc attgtttaaa acgttctgag acgattttta
      601 tatatggttt tggcgcatct tttgtagttg ctaccgactt ataccaaaag ttatcaagaa
      661 taggtcttaa tattcaactt gttcaagaaa cacatatttt tgctacatta ttagcaactc
      721 acaattcgaa cgacagtgtt attcttatta ctaacaatgg tacgcaaagt gaaatgcaat
      781 caatggttaa agttattgat gactaccata tacctataat tacaattact agtacaaggg
      841 ataatcccgt agcacaggca tcaaatattg ttttaactta tgggaaaact gatgaaaatg
      901 agatgcatat gggagcaaca acttcattat ttgctcaaat gttcactatt gatattttat
      961 attatcgtta tgttgcatta aattatcacg catcattaga tttcataaca caatctaaaa
     1021 tggctttaga taactatcgt aaacatttat caaatattaa ctttaaacat taacagtctt
     1081 atataaattc tttaatcatg tcatataata atttatgcat cattttcaaa gcttacatgt
     1141 cttttatctt gtataacata cgcatatgac caatgatttg ataaggccat tcaatttctt
     1201 atattaaaaa caaacgcagt tgattaacgg attcattcca tatatcaatt gcgtttgtta
     1261 ttttaataat gctatttttt attttaaagc ccacagtaaa aactaacgat aatattgcca
     1321 cttataagtt tttcacacta caacttcgat ttaagctata tttataaaaa aatgcgccta
     1381 ctcataacat tctttggcta tttaacttta ataagctagg gccaagtttg tcataagata
     1441 agcgcacatt ataatcatac tattttgctt ttacattatt tttattatat ctaataaaat
     1501 aataaatcgt tacaatcgtt aagaagatga caccaataag cactgatatt ctagtttcaa
     1561 cattaaatac cataccaacc aatactaata ttaagaataa gattgttaaa tagttagtta
     1621 ctgcgccacc aggcattttg aatgggtgac cttccaattc ttcagggtgt aattttctaa
     1681 aacgtaaatg actgaataaa atcataaacc aaggaatcat acctggtaaa atagacgcac
     1741 tataaacgta tacgaaaata ctcttcgctc catcgatata taaaggtaaa attacgttta
     1801 ataaagcacc aattaaaata ccaagagata ctgctacaac agtgtacaat ggtacaccat
     1861 ttctcatgat tttagtaaat attttcggca tttcaccttt atgagcaagt gtataaatca
     1921 tacgactcgc actgaaaata cctgagttac atcctgacat tgctgcagtt aatacaacaa
     1981 agttaattaa tccagctgca aatgtaatac cgattttagc aaatgttgca acaaacggac
     2041 ttccaatgtc tcctaattga ttccaaggat acactgaaac aataacaaaa atagcgccta
     2101 aatagaaaat taaaattctc cagataacac cattcactgc tttaacgata tttttctgag
     2161 gatctttagt ttcacctgcc gtaataccta tcagttccac accttggtat gaaccaatta
     2221 caattgataa tgcaaagaag aaaccaacaa taccatttgg cataaatccg ccatttgtcc
     2281 atagattaga aatacctacc gcatggccac catttccaaa accaaagaaa ataagaccaa
     2341 aaccagcaat aatcattaaa ataattgtta caactttaat taaagcaaac caaaattcaa
     2401 attctccaaa cgcttttacc gagaataaat tcgctgccat taataataaa atagcaataa
     2461 caccaggaat ccaagttggc aattccggga accaaaactt catatattct cctactgcga
     2521 tgacctcact cataccaact acaatccatt ggaatatatt actccatgct gtcatataac
     2581 ctgctgcagg atgtatataa tcacttgcaa atgttgcaaa tgatcctgtt gtagggttta
     2641 aataaatcat ttcccccatt gctctcatga ttaaaaataa aaagataccc gcaattaaat
     2701 atgcaaggat aactgatggg cctgtccatt taattgtact tgtcgcaccc atgaaaagcc
     2761 ccacgccaat cgtaccacca agtgcaatca tttgaatttg acgggcactc aatccccttt
     2821 gtagttcatt gttctccata acttcttacc ccatctctga ttgatactaa ataaatccat
     2881 aaaaaaactt attgccgtaa ttatactata aattatcgat tttggtacat taatatttgc
     2941 ataccatgca atcgtttaca gaactactat agcaggcttt tgcaactttg caaacaatta
     3001 ttttgtgatt tttctaaaaa attaaaaaag ttgctcaagt accgttgagt cacatacaac
     3061 ttcagctatt cttggtaatt atgatataat tttaataata ttaaaaagga gtatatcaat
     3121 gttaaaacaa atactgctac gtgctacaaa aatatcggta ctctttgctg tagcattttt
     3181 catcataaat tatatcggta tggaaaaacc tgacatccta tatttagttg gaagaactat
     3241 tattgcaaca cttgctttca tactcatttg tcttacagta tttactatta ttaattctcc
     3301 agagcgcaag attaaactcg gtacgacttt accgattgca ttaattattg gtatcatttt
     3361 cggcgccata tttttaacgg tacaaatcgg tgtcatcact gggttaatca tcggtgtgat
     3421 tgctacgttt atttgggaat tgattgaaaa aaataaagga ggtcgttcat catgacaaca
     3481 gcattaattg gtgtaattgt attaatctta ctaattatta gctttgtgcc aaactaccgt
     3541 gctatgaaat cagcaaaaga acaaggaagt aatgcaacaa gagctacaat tatggtaggc
     3601 atagatatta tcttaattgt acttatcatc gtgacgttat tattaaaata tatgtaacta
     3661 tttaaaccac cctatctcat acataaacat gaacgttcta ccatttcaca tacatatggt
     3721 cgtccattta atgaggtaag gtggttttct cgttaaagtt taccttgtaa tagtaaaaaa
     3781 tagccaattg ttgcgagtat taagtaaatc attgaaccaa caacaaaaga tcttacttgc
     3841 acgcgaaatg gtgtctgttt gttaaatata ggaccaatca ccaaatttcc aacgcctaaa
     3901 attactggac cagctagaaa aaacaatata atgtaaccca tcatgatacg cctcctattt
     3961 cgtaaagaaa cgcgctacaa tttgatcgta atcaatatct tttgctacat aatctacatt
     4021 agaaaagtcc tgtgcgcttg tcattttatt cgtattaaca ataacatcta atccagctgc
     4081 aatcgcggct gttgcaccat ttacagaatc ttcaatagcc aaacattgtg tcggcatata
     4141 atttaattgt tgtactgcag ataaatataa ttcagggtca ggtttaactt gttcaacatc
     4201 ttctctacca acaactacct ctatataatc gtctagacct aatgctttaa aggtgggcat
     4261 tatttccgct ttcacactac ttgtggcaat tgccatagga atgtgacgtt gctttaaaaa
     4321 tgccatcaat gatttaatcg tatcaatcat cggtaatgtt gtactagtaa gatgatgttc
     4381 ttcataaagt ttatctttat tttccgaacc aatcgcttta attaaatggt catgcaaatc
     4441 tgtagctgct cctccaatag aagaacgata aaaatcaatg cttataggat cggcattatg
     4501 catctctaaa tgtttattaa taacattaaa taaatgttgt tccgtatcta ttattgttcc
     4561 atcgaaatca aatataactg ctctatacat atatttactc ctctattttt tctctttatc
     4621 aaagcttaac atagtccgat gaccacttca ttctattagt gcaattataa cttttttgac
     4681 caatactttg ctaaaatagg ccctgttata ttatgaatga aactaaacac tgcgcctggt
     4741 actgctgcaa ttggattaaa atgcaatgct gctaatgaca cagctaaacc agagttctgc
     4801 attccaactt caatactgac tgctttttga tcttgtcgat ctaattttaa aagacgagct
     4861 aaccaatatc caatcgtata ccctaatacg ttatgtaaaa taactactaa aaatattaat
     4921 aatcctgtag ttaagatttg gtgcttactg ccacctacaa ctattgctaa aattaatgaa
     4981 atagcaacaa ctgatataat tggcaaagct gtagaagctt tttcagcaat tttacgatta
     5041 ataatttgca aaacaatacc taaagcaatt ggaattaata caacttgaac aactgaccac
     5101 aacatactca cgaaagatac ttccaaccat tcatttgcaa atagatatat taacgcaggt
     5161 gtaacgaatg gcgctagcaa cgtagagacc gtcgtaatag aaacagaaag tgctacgtta
     5221 gctttggcta aataactcat tacattactt gatgtcccac ccggacaaca tccgactaat
     5281 attacgccaa cagcaatatc aggtggtaaa tgaaaagact ttgcaattat aaatgctaat
     5341 gtgggcataa tactgaattg tagacagaca ccaataatta ctgctctagg tgctttaaag
     5401 accattttga aatcattagg tgtaattgtt aatcccatac ctaacataac aataccaagt
     5461 aaataaggta cccatttacc taatgtcgca acatgttgtg ggaaaataaa tccaataaca
     5521 gctgctacta acatccatac caaaaaactc ttcgtggcaa atatactcaa tttttgaaac
     5581 attgttaaca catcctcgtc gataatattg aaaaatatta ccataagacc agtaaggtta
     5641 caattacttt tctgaaaatt aagattattt agtcattctc ttcttattgc aaatatttta
     5701 tcactgataa acaattattt taaacttcat tcaaaagtct tagtgaataa agaaaaaaag
     5761 cgtggtaaaa tcaacttgaa caaaattaag tcacctttat aaatattaaa cgtgtattta
     5821 atgccatgta ttcctcggga ggtcctcaca tgttaattga taaattcgaa acatacatca
     5881 tcaacatcgc cggtttaaat gaccgtacta ctagaaaaaa attaagtaag ttatgcaaaa
     5941 gtgtacaatt ttgtgatgcg ttgcaattct caataaacaa acagtttaat cagtatgtat
     6001 tagaaattag cttaccaaag caacaacttc catacttcat ttcattttta agttttcatc
     6061 agtatagtat tttccaagta ctttcaccaa agaaaataaa tgaattactt gattctgata
     6121 atttatatca atctgcaaag cgtttcgata tcaatattga tggcttgcaa gatgctttca
     6181 ttaaagacaa agtcatcgat attatgaata tgttccaaaa tcacactgat atcacttata
     6241 cgttgaataa gtctcatgca catattatat gtacgccaga aatatttgct aaattgttac
     6301 atacgattgc aactagaaat atcgacattc tatctgccaa ttatagatcg tcatctatga
     6361 gcaaagctcg tatatcataa taaaagacac atctatacga tgatcattta caatgattgc
     6421 gtatagtgat gtgtcttttt ttgtattatt tatcttcgga cgattgatcc tcaaccattt
     6481 gttcaaatgc ttcacgaact tgtggtactg tcatacctac tacaacttgg atactcttac
     6541 cactctttac taatccgtgt gccatttgtt gatgcgtgaa atattccgta tctgcaactt
     6601 tactttcatc ataaacagtt aagcgtaatc ttgttgtaca gttagtaaca tctttgatat
     6661 tttctttgcc gcctaaaccg tcgaggtaat atgcagcttt gtcttcgtat tcattaccag
     6721 atgaagcacg tttagaatct acgctatcac cttttttatt tttgtaatct tgcttagaaa
     6781 ataatttaac ttcctcttct gttttcttac gtccaggtaa tggaatatca aatttcaaaa
     6841 ttaagaatct gaataagaag aagtaaatcg ctacaaatat cagaccgata ataacttgtg
     6901 caacataagt accccaatgt tctttaccta atggcaacca gtttgttgaa ataaagtcta
     6961 acaagccacc acccatgtta cctacaacac caaatgcgta cattaaagta tccattgatg
     7021 ctgctaaaac tgcgtgtaaa acaaataagt atggtgcgat gaataagaat gtaaactcta
     7081 atggctcagt aatacctaca acgactgctg ttaatgtggc aggaataagt agtgccgcca
     7141 cctttttacg attttcttta ggtgtcgtag catacattgc taaagcaata cctaaacatc
     7201 caaatacttt cccatttcct tgaagcataa agccataagg gaattgctct tttaatggct
     7261 tagtactctt agcaaactca tttaaatgtt gtaaccattc agctttcaag ccgtgattga
     7321 ctactactgg tccaacttca attggtgcat aaataaagtg atgtaatcct gttggtatta
     7381 atacacgttc taagaagtga tacaaccata caccgatata gcctgaagca atgataaagt
     7441 gttgcattga accaataccg tgctgaacag ttggccaaat aacacaagta attgctgcta
     7501 atggtaacat cacaaaaaat gaaatcgtaa ctacaaatgt taaaccttgg aatacaccaa
     7561 ccatttctgg taatcgttta ctatagtatc tattgtgaat ccaagtaata atagctgaaa
     7621 taataatacc acctagaata ttcgtatcca atgtggcaat acctgcaatt gattttaaac
     7681 caggtacatt ttcaacgcct ttttctaaat tagcgccaaa cgtatgtggc cattgtgtta
     7741 aaatggcatt tataaatgta ttaaacatta agtaacccat caatgctgca agtgctgcat
     7801 gacctggtgc ttttttagct aaagaaagtg gtagacctac aacaaataca acttccatat
     7861 gcgtaaaaat aacccagcca ccagattcta ttactgacca aaatttaaac caaaacgtat
     7921 gttgatctgc taaactcccc atgattgtag gatttttaaa taatgtcgca aagccaagca
     7981 ctatcccaaa gaaagcgaac attaataccg gtacaatcat tgcactaccg aaacgcttta
     8041 tcgcattcat cttctattcc ctccatatca tctttcctaa caatacatct aattagattc
     8101 atttataaat agatgtctta ctatttaaat ataatatata gtaaacgctt acacacctac
     8161 aacgacattg acgtattttg aaagtatttt gtataatcag attatctttt catatagtga
     8221 aaattttttc acgaccttat atatgacatc gttgtatttg taatacattc gttttaaacg
     8281 cataatcaaa cctatatcaa tacacaaata tatataatga catacaagat ttt
//
LOCUS       DAKTIP010000115         8166 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAKTIP010000115 DAKTIP010000000
VERSION     DAKTIP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.115"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(516..1433)
                     /locus_tag="O0305_001858"
     CDS             complement(516..1433)
                     /locus_tag="O0305_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCY0274999.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMTHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            1524..1625
                     /locus_tag="O0305_001859"
     CDS             1524..1625
                     /locus_tag="O0305_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275000.1"
                     /translation="MVINEIDFYRVGRENIVIAVYNEHIKFLFILIS"
     gene            complement(1703..3208)
                     /locus_tag="O0305_001860"
     CDS             complement(1703..3208)
                     /locus_tag="O0305_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCY0275001.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAISPLIIKAMKGIR"
     gene            complement(3549..4049)
                     /gene="queF"
                     /locus_tag="O0305_001861"
     CDS             complement(3549..4049)
                     /gene="queF"
                     /locus_tag="O0305_001861"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCY0275002.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(4069..4935)
                     /locus_tag="O0305_001862"
     CDS             complement(4069..4935)
                     /locus_tag="O0305_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCY0275003.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
     gene            5736..6134
                     /gene="nrdI"
                     /locus_tag="O0305_001863"
     CDS             5736..6134
                     /gene="nrdI"
                     /locus_tag="O0305_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCY0275004.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            6097..>8166
                     /gene="nrdE"
                     /locus_tag="O0305_001864"
     CDS             6097..>8166
                     /gene="nrdE"
                     /locus_tag="O0305_001864"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCY0275005.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKL"
BASE COUNT         2903 a         1253 c         1342 g         2668 t
ORIGIN      
        1 gtagttgact gcggggcccc aacatagaga aattggaacg ccaatttcta cagacaatgc
       61 aagttggggt gggccccaac ataaagaaat actttttctt tagaaattag tatttcttat
      121 gcatgagtgt aactcatgca ttcatatttt taagtacaca ttagctgtga ctaatgataa
      181 agaatcgcta cataatcaat cattagtcgt tctttatcat ttccgtcccg ctctcaataa
      241 atgttagttt atcttattat tataaatcgg atgaatgtgt taatctatgg cagattacac
      301 gtcatccgat tttttataga atttgaaaaa gacgcataaa ccactatgat ttaaaataca
      361 acatcaatca ttttagtggc atgcgccaaa attatatgtc tgtttttgaa acagggtaat
      421 agcttaaagc taataaaaac gaatataagg tgcgttgaat cttatgatta cactccaaac
      481 ctaatataat atcgggttaa gatcattccg gatgcttaca aatcattgac agtaagtaac
      541 tgaatggcat ttggtataac ctcaatatca ataggtgttt ctaatgaaat ttcgccatca
      601 atatcaactt tcattgctgg atctgttgta agtgaaatct ttttaccagg tatatgctca
      661 ataccttgag taatttcatt ccaattcatg ctatcacgct ttttaaaaat atcatttaaa
      721 atactgaaac tttgttcatt aaaaatgaaa gtgttcagtt caccatcttg aggagacaaa
      781 tcagtcaatg gtatacgact accaccaatg aatggaccat ttgctgttag tatcatggtc
      841 gtttcgccag aatatgtctt atcatctatt gataattgat aattaaattg tgttggattt
      901 agcagtgttt tgacagttga tccaatataa ctcaatttac caaatatatc ttttgaacca
      961 tcttgtacgt tttcagcgtt ttgaacaatg agacctaagc caacaaagtt gagtgcatat
     1021 tgattattta ttttaattac atcgtatgta ccaacttgtg cagaaatcat ttgttcacta
     1081 gcttgtttat gattaggtgc tatatttagc gtttttgtaa aatcattaaa agtaccgcct
     1141 ggtaaaatgc caatagggag ttgaaggtca tgtgtcataa caccgtttat aagttcgtta
     1201 accgtgccat caccgccaag aataaataat atatctacat cttttgcata gtttttagtt
     1261 ttgatttctt ggcaatattt aataatgtca ccttcgtttt cactcaattg aatagaaaga
     1321 tgcttacaaa ttgaacttaa tgctgttgta acttccccaa taccttgatt aatatttttt
     1381 aatccactgt gttcatggta aaagaggaca ccatgtgtat atttattttc catagtttag
     1441 cctactttct aaaaattggt tcattaaata tatataccca cttttaattg ttaataccaa
     1501 aaatatgttt ttaaatagag aaaatggtaa taaatgaaat tgatttctat agagtgggac
     1561 gagaaaatat agttatagct gtctataatg agcatattaa gtttttattt atactgatat
     1621 cttgaattta attaatagaa acctataaaa aaacagtaag ccatttaaat gacttactgt
     1681 tttttgaatt aggccaacaa tattaacgta tacctttcat cgctttgatg attaaaggtg
     1741 agattgctaa tacaattgtt gtaacaataa ttgcaacaac acctaggaaa ataaagtaat
     1801 ttgtttgacc tagtggttct attaacttaa ctaaagtacc attgattgct tgtgcagaag
     1861 cgttagttaa gtaccaaata ctcatcattt gggcattaaa tgctttaggt gctaacttaa
     1921 cagcagcact attacccgtt ggtgataagc atagctcacc gataacacaa ataatgtacg
     1981 ataaaataac ccagttaact gaaaagtttg atgaacctga tgcataacct acaataccaa
     2041 ttagtatgta tgacgcacct gctaagaacg taccaattgc aaattttact ggcaggctag
     2101 gttgtttagt tccaagcttt tgccataaaa gtgaaataat tggagctagt aataaaataa
     2161 ataatgggtt aattgattgg aagatcgctt caccaaagtt tgttttccaa ccaaataagt
     2221 ttaatttcat atctgaatgt tcaattccat atatgtttaa tacattagac ccttgttctt
     2281 gaatagccca gaacaccatt ccaagaataa ataatggaat aaatgcttta acacgagaac
     2341 gttcagtatc agtgacatct ttacttctaa taattaaagt gaagtaaatg attggtaatg
     2401 caatacctaa tactaaaaca gtattactaa ctaagttaaa tgataatgag ttagttaatg
     2461 caccaataac gataattaat acaattgcta aaacaacact tccgataata agaccatact
     2521 ttttcttttc agctggtgtc aatgggttag taggtttcat accaacgcta cctaagtttt
     2581 tgcggttgaa aagtacatac catactaaac ctaatgccat accaactgct gcaatcaaga
     2641 atccgccgtg gaagttttta acattaacaa agtgttgcaa aataataggt gataataatg
     2701 cacccatatt aactgacata tagaaaataa caaaacctgc atccatacgt ctatcatttt
     2761 caggatataa acggccaacg atatttgaaa tgtttggctt cattaaacct gaaccaataa
     2821 tgatgaagaa cattgatgtg aataagccga ttaatgcaaa tggtaagctt aaacaaatat
     2881 gtccgataat aataaagact gcacctaata aagtagcgcc tctagtgcct gtaattctgt
     2941 cagcaatcca tccgcctggt attgatgtca tatagattaa tgaaccataa actgacataa
     3001 ttgacatagc tgttgtttta tcaattccaa ggccattatc tgttacggca aagtacatgt
     3061 agaaaatgag tagggcacgc atgccataat aactaaacct ttcccagaac tctacaaaga
     3121 agagtacgcc tagtcctcga ggatgcccga aaaatcctgt ttgaggtatg tcttgaattt
     3181 gatttccatg ggagttttgt tgtgtcatgt atacatccca tcctttcttc ccctaataca
     3241 atagtttaag taaatttgat tgtgccttat caactaaata ttccaacaaa tcacaaaaaa
     3301 ataaaaatat tgagaatatt ctgttattaa gcgcgcacaa ttaattagag ataatataaa
     3361 aatatactta ttgacgttaa agtgcaatag gtttctataa aaatgttgtt taattcgtgt
     3421 actaatattc ttttggtgta ctttggtata aaatgttatt ttaatgtaac ctattatgta
     3481 tgctaaatga aaaacacacc aataagttag gaaaatcaac ttattggtgt gtttattaaa
     3541 tgatacattt aacgattatc tattttttcg ggatataaat catgattcat caaacgatgc
     3601 tcagccattt tttcatattt agaatttgga cgtccatagt ttgtataagg atcaatagaa
     3661 attccaccac gtggtgtgaa cttgccccag acttcaatat aatgtgggtc cataagctct
     3721 atcaaatcat tcataataat attcatacaa tcttcgtgaa aatcaccgtg atttctgaaa
     3781 ctaaataagt ataatttcaa agattttgat tcaaccattt taacatttgg aatatatgaa
     3841 atatagatag ttgcaaaatc tggttgccca gtaattggac ataatgatgt aaattctgga
     3901 cagttgaatt ttacgaaata gtcacgacct tgatgcttat tatcaaacga ttctaataca
     3961 tcaggacgat agtcaaaatt gtaagtattg tcttgatttc ctaataaagt tatatcttgt
     4021 aattcatctt gttgacggcc atgtgccata taaagcgctc ctttaaattt atttttttat
     4081 tattttggcg tctcggcgtg ctttttcaaa catgtaataa cttgcaccga taataacgac
     4141 gtaacctaat gttgcataga aatctggaga ttctccgaat agaataaatc caagtattgc
     4201 tgtgaaaatt atagatgcat acgtaaaaat agaaatatct tttgctgctg caaaactata
     4261 tgctaaagta acaccaattt gacccacagc ggcagctaag ccagccccta atagataaag
     4321 tatttgcatc tgactcattg gttcataagt atatgcagtg aaaggtatta aaacgatgac
     4381 agaaaataag gagaagtaaa atactatagt atatggtgct tctcttgtac taagtgctcg
     4441 aacacatgta tatgctgatg ctgcaaaaat acctgagaat aagccagcta atgatggaat
     4501 catagatgat gaaaattcag gtttcactat taaaagcata cctaaaatag caattatcat
     4561 tgctgtaatt tgatacttcc ttaccttttc atgtaagaaa acaatgctta ataaaatcgt
     4621 ccagaaagga ttgagtttca ttaatgaatc ggcatcacta agtaccatat gatcaatggc
     4681 ataaatattt aacaatacac caataagtcc aagtgttgat cgtgttatta ataagggttg
     4741 acttgaaagt ctgccaaaca ttggctgatg gtatttatat ataaaaaata atggaataaa
     4801 cattgctact aagtttcgtg ctaatgattt ttgaaaaaca ggaaggtcac ctgcaagtct
     4861 gaaaaacact gacataaaac tgaaaccaat agccgaaatt aaaatggcaa tgataccttt
     4921 tactttagga ttcaatttta tcgcctcttt tatataaaat taacgtattt atattagcat
     4981 aaaacaacat gttgtgcata aatagttgaa atttactata aaaagactat aatagactgt
     5041 agcgaacaaa cgttctgtgt ttatttgtcg gaataatagg gcattacact tttatgaatg
     5101 tttgtgttat tacataaaac aaatatcaat tcagtatcaa gctaataagc tttttcttga
     5161 tttctgttga tacaattgag attgacacag atttaaaaaa atcaagtgat atctactaaa
     5221 aaattttttt aaatttgttc aagtttttct aatttagtat tggtgcctag ttggaacgtt
     5281 ttacgaacat tcgattagaa aatggcactt taaatcatag tgtgtcttat gtataatgaa
     5341 acacataata tagtgttggt gaaacgaaaa agacacaata tcttgtgttt tgtatgcaaa
     5401 tgctttattt atgaagaaat tacatttaaa agtaatttaa cacagaaatt taatagttat
     5461 tatcaattaa tagtcatatt tttagaaaat gtactgagca aatggaagat atccaatgat
     5521 gtaaacacta catatagtga tttttataca ttcaacccat ataagctact attttctcaa
     5581 atataaatct atgcaattgg tttacatttg agaaaataag tagcttcatt atagttaata
     5641 caatgctgag ataaccatag taaccatgtt gttaaagcat tttttaattg gaatgactac
     5701 tttatttaaa agggttgaag aaagaaggtg atccaatgaa aataatatat ttttcattta
     5761 ctggaaatgt ccgtcgtttt attaagagaa cagaacttga aaatacgctt gagattacag
     5821 cagaaaattg tatggaacca gttcatgaac cgtttattat cgttactggc actattggat
     5881 ttggagaagt accagaaccc gttcaatctt ttttagaagt taatcatcaa tacatcagag
     5941 gtgtggcagc tagcggtaat cgaaattggg gactaaattt cgcaaaagcg ggtcgcacga
     6001 tatcagaaga gtataatgtc cctttattaa tgaagtttga gttacatgga aaaaacaaag
     6061 acgttattga atttaagaac aaggtgggta attttaatga aaaccatgga agagaaaaag
     6121 tacaatcata ttgaattaaa taatgaggtc actaaacgaa gagaagatgg attctttagt
     6181 ttagaaaaag accaagaagc tttagtagct tatttagaag aagtaaaaga caaaacaatc
     6241 ttcttcgaca ctgaaatcga gcgtttacgt tatttagtag acaacgattt ttatttcaat
     6301 gtgtttgata tttatagtga agcggatcta attgaaatca ctgattatgc aaaatcaatc
     6361 ccgtttaatt ttgcaagtta tatgtcagct agtaaatttt tcaaagatta cgctttgaaa
     6421 acaaatgata aaagtcaata cttagaagac tataatcaac acgttgccat tgttgcttta
     6481 tacctagcaa atggtaataa agcacaagct aaacaattta tttctgctat ggttgaacaa
     6541 agatatcaac cagcgacacc aacattttta aacgcaggcc gtgcgcgtcg tggtgagcta
     6601 gtgtcatgtt tcttattaga agtggatgac agcttaaatt caattaactt tattgattca
     6661 actgcaaaac aattaagtaa aattgggggc ggcgttgcaa ttaacttatc taaattgcgt
     6721 gcacgtggtg aagcaattaa aggaattaaa ggcgtagcga aaggcgtttt acctattgct
     6781 aagtcacttg aaggtggctt tagctatgca gatcaacttg gtcaacgccc tggtgctggt
     6841 gctgtgtact taaatatctt ccattatgat gtagaagaat ttttagatac taaaaaagta
     6901 aatgcggatg aagatttacg tttatctaca atatcaactg gtttaattgt tccatctaaa
     6961 ttcttcgatt tagctaaaga aggtaaggac ttttatatgt ttgcacctca tacagttaaa
     7021 gaagaatatg gtgtgacatt agacgatatc gatttagaaa aatattatga tgacatggtt
     7081 gcaaacccaa atgttgagaa aaagaaaaag aatgcgcgtg aaatgttgaa tttaattgcg
     7141 caaacacaat tacaatcagg ttatccatat ttaatgttta aagataatgc taacagagtg
     7201 catccgaatt caaacattgg acaaattaaa atgagtaact tatgtacgga aattttccaa
     7261 ctacaagaaa cttcaattat taatgactat ggtattgaag acgaaattaa acgtgatatt
     7321 tcttgtaact tgggctcatt aaatattgtt aatgtaatgg aaagcggaaa attcagagat
     7381 tcagttcact ctggtatgga cgcattaact gttgtgagtg atgtagcaaa tattcaaaat
     7441 gcaccaggag ttagaaaagc taacagtgaa ttacattcag ttggtcttgg tgtgatgaat
     7501 ttacacggtt acctagcaaa aaataaaatt ggttatgagt cagaagaagc aaaagatttt
     7561 gcaaatatct tctttatgat gatgaatttc tactcaatcg aacgttcaat ggaaatcgct
     7621 aaagagcgtg gtatcaaata tcaagacttt gaaaagtctg attatgctaa tggcaaatat
     7681 ttcgagttct atacaactca agaatttgaa cctcaattcg aaaaagtacg tgaattattc
     7741 gatggtatgg ctattcctac ttctgaggat tggaagaaac tacaacaaga tgttgaacaa
     7801 tatggtttat atcatgcata tagattagca attgctccaa cacaaagtat ttcttatgtt
     7861 caaaatgcaa caagttctgt aatgccaatc gttgaccaaa ttgaacgtcg tacttatggt
     7921 aatgcggaaa cattttaccc tatgccattc ttatcaccac aaacaatgtg gtactacaaa
     7981 tcagcattca atactgatca gatgaaatta atcgatttaa ttgcgacaat tcaaacgcat
     8041 attgaccaag gtatctcaac gatcctttat gttaattctg aaatttctac acgtgagtta
     8101 gcaagattat atgtatatgc gcactataaa ggattaaaat cactttacta tactagaaat
     8161 aaatta
//
LOCUS       DAKTIP010000116         8140 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAKTIP010000116 DAKTIP010000000
VERSION     DAKTIP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.116"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            127..942
                     /locus_tag="O0305_001865"
     CDS             127..942
                     /locus_tag="O0305_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HCY0275006.1"
                     /translation="MHYIKFIESKDNTKLYMKVNDIQDAKANIIIAHGVAEHLDRYDE
                     ITAYLNEAGFSVIRYDQRGHGRSEGKRAFYSNSNEIVEDLDAIINYVKSNFEGKVYLI
                     GHSMGGYTVTLYGTKHPNTVNGIITSGALTRYNNKLFGNPDRNISPDTYIENNLSEGV
                     CSDLEVMEKYKLDDLNAKQISMGLVFSIMDGVRYLKDNAQQFTDNILILHGKEDGLVS
                     YVDSLQLYQEIGSAHKSLHIYDRLEHEIFNESSYNRTIFNEVIEWLETELTYN"
     gene            1264..2442
                     /locus_tag="O0305_001866"
     CDS             1264..2442
                     /locus_tag="O0305_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCY0275007.1"
                     /translation="MSKRSSVVAHYKNGKFQKEFFIQNNKNGYNYLLKYLNDLDHPQL
                     IFESTGIYSRGMERFCCVNQINYIQMNPLEAKFKTSALRSWKTDQADAHKLACLGPTL
                     KQTGSLPIHELIFFELRERARFHLEIENEQNRLKFQILELLHQTFPGLERLFSSRYSI
                     IALNIAEIFTHPDVVLDIDKDVLITHIFNSTDKGMSMDKATKYALQLRVIAQESYPNV
                     DRHSFLVEKLRLLIQQLKQSIHHLKQLDDAMIQLAQQLDYFENIHSIPGIGKLSTAMI
                     IGEIGDIKRFKSNKQLNAFVGIDIKRYQSGHTHCRDTINKRGNKKARKLLFWVIMNII
                     RGQHHYDNHVVDYYYKLRKQPNEKPHKTAIIACINRLLKTIHYLVMNHKLYDYQMSPH
                     "
     gene            2702..3394
                     /locus_tag="O0305_001867"
     CDS             2702..3394
                     /locus_tag="O0305_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0275008.1"
                     /translation="MTKTYDVWWQKGQESDDDMARDHQEAWERTIKMLDTSDIEGKTI
                     LDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKH
                     VFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYG
                     ATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESF
                     LIKASVKEGTEK"
     gene            3391..4944
                     /locus_tag="O0305_001868"
     CDS             3391..4944
                     /locus_tag="O0305_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0275009.1"
                     /translation="MRKCVLMTVAASATLLLAGCGNGQKEDKDVTVSLPTEAKADKLD
                     AQGYDAAMPVYSAVYDALVKYDKDKGIKAGLADKWSVDESGKVYEFHLKKNVKFSDGS
                     ALDAKDVKFSIDRAKAMNKDSTVETLKKLDKVVVKNEHVVQIRLKSPSNQVLNELTQV
                     RPLRIMSPHSVEDGKVNGKFEKAIGTGAFVVDKTGKEKTTMKPNKYFDNGHPVNYHLA
                     FQTIEDGDSRNSAVQSGSVDISGGALGMLSDEQIKQDKKNKNLTIEDRPSTVSHFMAF
                     NPKNDVLNQRTIREAISKSIDAKDIAGKSVNGLFQKNVQFVTKNNQQPHDYDMKAAER
                     LLKSEGYHKNDDGIFEKNGKPLSFNLVIQTAEFPNWKDKAEKVQRQLKQAGIKLNVKT
                     LDSQSYYDTLWTKKDYDLIFYRTYSDALMPYNFISSVFKNNDGQPGVLADDETLTKQL
                     DDFPSTVSKEDQQCSFDDIFKHFNQQYYGVPIAYPNETFVVSDKVKQFKFSGLTDAPI
                     DYKALKVNE"
     gene            4947..5903
                     /locus_tag="O0305_001869"
     CDS             4947..5903
                     /locus_tag="O0305_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275010.1"
                     /translation="MLKRTIKFILYLIVSSFIIFILVEKTSGNPAILYLQRHGYTSIT
                     QDNIEAAQHQLGLGQHVLLRYIDWVGHALTGNLGYGFSTNEAVTAMIMEAIVPTLVLI
                     IVSSCIMLPFGYIVGYFVGTRPHTRYANGIRGFAQVMTSMPEYWLAILFIYYLGVRWQ
                     LLPFVGSDSWQHFVLPIFTIVVIEGCHILLMTAHLITQTLDQDAYQLAQLRHFSLKAR
                     IIVQIKEIFAPLMTISINSIIHLIGKAVILEVIFSMSGIGKLLINAINQRDYPLIQGI
                     VIFIIVFIMLMNYLGDVIILKNEPRLRRRHTQQSGNEKRGTM"
     gene            5903..6670
                     /locus_tag="O0305_001870"
     CDS             5903..6670
                     /locus_tag="O0305_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275011.1"
                     /translation="MKKYQTYIAIGSLLSLMVVLITYGLMQDTQHLNPLESPNGQHWL
                     GTDQLGRDFLVRLIVGSLVTLSLTGIVILLSVCMGLIFGLIAGIERRWLDQIIMFVAD
                     MLLAIPSFIIALVILSLVSNSMIGLILALTIGWIGRYLRYFRNLTRDIQKRPFVQYAR
                     LSGNSTFKTTVTHVIPHLLSSIFALVTADFGKMMLSISGLAFLGLGIKPPTPELGTIL
                     FDGKSYFNGAPWLFFFPGVLLGGFALLCQIINKKITQ"
     gene            6637..7404
                     /locus_tag="O0305_001871"
     CDS             6637..7404
                     /locus_tag="O0305_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275012.1"
                     /translation="MSNYQQKNNAVNTVVNVNQLSILDQEKVLLKDVDLTVTKGAFHC
                     IIGESGSGKSLLTRTILGMKQSQLCYQGDIDIDLTQTDAVFQDVQSNMFQNITLAKHF
                     QYIYEANRTHLTKQRIKEDVLQMMQLLGLRQGEQLLERYPFELSGGMAQRVAFIMSLI
                     RRPNYLFLDEPTSALDQENIKKFMHYLLRAQERYQMTIVFITHDINLVKDCATHISIM
                     QQGKLIENGEAASILTKPTHNYTKKLITIAHRRQPYA"
     gene            7397..8032
                     /locus_tag="O0305_001872"
     CDS             7397..8032
                     /locus_tag="O0305_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002490830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0275013.1"
                     /translation="MLKIERLTKYIDTQLIFKEISCTINDQHLLISGESGCGKSTLAK
                     IIAGLDTDYQGELYLNGRLRESYTSKEWMKHIQYVPQYQRDTLNQRKTVLATLLEPLK
                     NYKVNKQRYTSSIEAVLDQCNLPHDILNHKVSTLSGGQFQRVWIAKALILEPEILILD
                     EATTNLDVINEEAILQMLISLKMTQLIIISHDTYVLSQFEGIQLQLNKLNN"
BASE COUNT         2870 a         1252 c         1547 g         2471 t
ORIGIN      
        1 taaaaaacat gggcttaaag ggttaattta tatagtatta ctgcaaacat tgatttgggt
       61 caaaaattag gggtattaaa aaaatgaatg aatcataatt ttatcaagct gattggagag
      121 gttaaaatgc attatataaa atttattgag tcaaaagata atacaaaact ttatatgaaa
      181 gtgaatgata ttcaagatgc aaaagcgaat atcattatag ctcatggtgt ggcagaacat
      241 ttagatcgtt atgatgagat aacagcatat ttaaatgaag cgggttttag tgttattaga
      301 tatgatcaaa gagggcatgg tcgttctgaa ggcaagcgtg ccttttatag caatagtaat
      361 gaaattgtcg aagatttaga tgcgataata aattatgtga agtcaaactt tgaaggtaaa
      421 gtttacttaa tcggtcatag tatgggtggt tatacagtca ctttatatgg aacgaaacat
      481 ccaaatacag tgaatggtat tataacttct ggagcattaa cacgttataa taataaacta
      541 tttggcaatc ctgatagaaa catatcacct gatacttata tagaaaacaa tttaagtgag
      601 ggggtatgtt ctgatttaga ggtaatggaa aaatataaac ttgatgattt gaatgcgaaa
      661 caaatctcta tggggctcgt cttttcaata atggatggtg ttaggtattt gaaagacaat
      721 gctcaacaat ttacagataa tattttgata ttgcatggca aggaagatgg gctagtaagc
      781 tatgtagatt ctttacagct ttatcaagaa ataggatcag cacataaatc attacacatc
      841 tatgatcgtt tggagcatga aatatttaat gaaagttctt ataatagaac tatttttaac
      901 gaagttattg aatggcttga aacggaatta acttataact aaaacagtat agttccgtgt
      961 atttgattat aagaaattat gaggatatta aacatactaa gattagctat gaagaaatct
     1021 atgacgatag attttttcat agctattttt tatagttata gagaggagta gactgtccag
     1081 actcttggat tttaaatccg taaaaaaaac aagtcagctt tactctcacc ttttgaaatt
     1141 cgtttgtagt atgttgggtt cttgaaaccg tgtataggaa aatgaaatga gaaaggttaa
     1201 gtaaagtttt tagcttctca actattcaaa ggaggttttt ttatcgatta cttaggtgtt
     1261 gatattagta aaaggagtag tgtagttgca cattataaaa atggaaaatt ccaaaaagag
     1321 tttttcatcc aaaataataa aaatggctac aattatttac tcaagtattt gaatgactta
     1381 gaccacccac aactcatttt tgaatctaca ggtatctatt caagaggtat ggaacgattt
     1441 tgttgtgtaa atcaaattaa ctatattcaa atgaatccgt tagaagccaa atttaaaacg
     1501 agcgctctaa gatcatggaa aactgatcag gcagatgctc ataagcttgc ttgtttagga
     1561 ccgacgctca aacaaacagg cagcttacct atacatgagt taatattctt tgaattaaga
     1621 gaacgtgccc gttttcatct agaaatcgag aatgaacaaa atcgacttaa atttcagatt
     1681 cttgaattac tccatcaaac attccctggt ttagaaagat tatttagtag tcgatattca
     1741 atcattgcac tcaacatcgc agaaattttt actcatccag acgtggttct tgatatcgac
     1801 aaggatgtac ttattacaca tatattcaat tctacagata agggaatgtc aatggataaa
     1861 gctacaaaat atgcacttca attaagagtg attgctcaag aaagctatcc taatgtcgat
     1921 agacattcct ttctagtcga aaaattacgc ttacttattc aacaattaaa acaatctatt
     1981 catcatctca aacaattaga tgatgccatg attcaattag cacaacaact cgattatttt
     2041 gaaaatattc attcgatacc tggtattggt aagctaagca cagctatgat tattggggag
     2101 attggtgata ttaagcgatt taaatcaaat aaacaactca acgcttttgt aggcattgat
     2161 atcaaacgat atcaatcagg tcatacacac tgtagagata ccatcaacaa gcgtggtaat
     2221 aaaaaagcga gaaaactttt attttgggtg attatgaata taataagagg gcagcatcat
     2281 tatgacaatc atgtcgtcga ttattactac aaactaagaa agcagcctaa tgagaaacct
     2341 cataagactg ccatcattgc ttgtataaat cgattattaa aaacgattca ttatctggta
     2401 atgaatcata aattgtacga ttatcaaatg tcaccacatt agccaaacgt acaattaaat
     2461 atattttaat accttattca aaaaattaaa atgaacggtt tagttaagta atgcttattt
     2521 taattataag tacttgacta atcgtaagaa agagcctagg acataaatca atgtctcgcg
     2581 accacagctt aatttttggt gttcattcat gactttaaaa aatccttatt gcataaatgt
     2641 acatagtgta gtactattca aaacgtaatt attacgattt gaattaagcg aggagaatga
     2701 aatgactaag acttatgacg tttggtggca aaaaggtcaa gaatcagatg atgatatggc
     2761 acgagaccat caagaagctt gggagagaac aataaaaatg cttgatacat ctgacatcga
     2821 agggaaaacg attttagatg tgggatgtaa tcaaggcgga tttttacgac agttatacga
     2881 tacaacaccg tttaaaaaag gtgttggcat agatttagca cgtttatctt tggaaaaggc
     2941 agagacatta aaaggacaac gtccacttac atactattta acagataaac cgcaagaaac
     3001 gaagcacgtg tttgatacgg cagtaagtac gtctgtcttg tacttaatag aagatattcc
     3061 gcaacatgca aaagatttaa aagaggtatt gaaaccaggc ggtgtttatt acgcttcatt
     3121 cgcggattta actaataacc caagtcgtca gtttatggat gacacgatta atcaatatgg
     3181 tgcaacacct tctcagaatc actctctaaa acatatcgtt gatagctttg tggatgcagg
     3241 atttgaagtt gcagtaatga aagagcatgt acctgacgtg attgatttaa cacattatag
     3301 cgatttttat ttatcaccga atgattattt acaaacacta tatgaagaat cgtttttaat
     3361 aaaagcaagt gtgaaagaag gtactgagaa atgaggaaat gtgtattaat gacggtagca
     3421 gcaagtgcta cgctcttatt ggcaggttgt ggcaatggtc aaaaagaaga taaagatgtt
     3481 acggtatcgc tacctactga agcaaaggcg gataaacttg acgcgcaagg ctatgatgca
     3541 gcgatgcccg tttatagtgc agtgtatgat gcattagtta aatatgataa agataagggt
     3601 attaaagcag gtttagcaga taaatggagc gttgatgaat cagggaaagt ttatgaattc
     3661 catttgaaaa agaatgttaa attctcagat ggttcagcat tagatgctaa ggacgtgaaa
     3721 ttctcgattg atcgtgcgaa agcgatgaac aaagattcga ctgtagaaac gttaaaaaaa
     3781 ttagataagg tcgttgttaa aaatgagcac gtggtccaaa ttagattgaa atctccttca
     3841 aatcaagtgt taaatgaatt aacacaagtg agaccgttgc gtattatgag tccacattca
     3901 gtagaagatg gtaaagtaaa cggtaaattt gaaaaagcga ttggaacagg tgcatttgtt
     3961 gttgataaaa ctggtaaaga aaaaacgaca atgaagccaa ataaatattt tgacaacggt
     4021 cacccagtca attatcatct tgcattccaa acgattgaag atggggactc aagaaattct
     4081 gcagtacaaa gtggttctgt agatatttct ggtggtgctt taggtatgct ctcagacgaa
     4141 caaatcaaac aagataagaa aaataagaac ttaacgattg aagatagacc tagcacagta
     4201 agtcacttta tggcatttaa ccctaaaaat gatgtattaa atcaacgcac aattcgtgaa
     4261 gcgataagta agagcatcga tgcgaaagac attgcgggca aatctgtaaa tggtctgttc
     4321 cagaagaacg tacaatttgt gactaaaaat aatcaacagc cacacgatta tgatatgaaa
     4381 gcggctgaaa ggttacttaa atcagaagga tatcataaaa acgatgacgg catctttgaa
     4441 aagaatggca aacctttatc atttaactta gtcattcaaa ctgcagagtt cccaaattgg
     4501 aaagataaag ctgaaaaagt gcaacgtcag cttaaacaag ccggtattaa gttaaatgtg
     4561 aaaacgttag attcacaatc atactatgat acattatgga cgaaaaaaga ctatgatttg
     4621 attttctata gaacgtattc agatgcatta atgccttaca actttataag ttcagtgttt
     4681 aaaaataatg atggtcaacc aggggtgtta gctgatgatg aaacattaac gaaacagcta
     4741 gacgatttcc catcaaccgt atcaaaagaa gaccaacagt gttcatttga tgacatattt
     4801 aaacacttta atcaacaata ctatggtgtg ccaattgctt atccaaatga gacgtttgta
     4861 gtgagtgata aagtaaaaca attcaaattc tctggactta cggatgcacc aattgattat
     4921 aaagcgttga aagttaatga atagcaatgc tcaaacgtac aattaaattc atactttatt
     4981 taatcgtaag ttcgtttatt atcttcattt tagttgagaa gacatctggt aatccagcga
     5041 ttctgtatct acaacgtcat ggttatacgt cgattacgca agacaatatt gaagcggcac
     5101 aacatcaact tggcttagga caacatgtgt tactaagata tatcgattgg gttggacatg
     5161 cactcacggg caacttagga tacggcttta gtacgaacga agcagttacc gctatgataa
     5221 tggaagccat cgtgccgacg cttgtgctaa tcattgtctc tagttgtatc atgttgccat
     5281 ttggctatat tgttggttac ttcgttggga cgcgtccgca tacacgttac gctaatggaa
     5341 ttcgtggatt cgcacaagtg atgacctcaa tgccagaata ctggttagct attttattca
     5401 tttattattt aggcgtacgt tggcaattgt taccatttgt aggtagtgat tcatggcaac
     5461 actttgtgct gccaatcttc acaattgttg ttatagaagg gtgtcatatc ttattgatga
     5521 cagcacatct gattacacaa acgttagatc aagatgcgta tcaactggcg cagttaagac
     5581 atttttcgtt aaaagcgcgt atcatcgtac aaattaaaga gatatttgca ccactaatga
     5641 cgatttcaat taacagtatc attcatttaa ttggaaaagc cgtaatacta gaagtcatct
     5701 tcagcatgtc tggtataggt aaattgttga ttaatgctat taaccaacga gattatccac
     5761 tgattcaggg cattgtcatc tttatcattg tctttattat gctaatgaat tatttaggcg
     5821 atgtgattat tttgaagaat gaacctagac ttcgacgacg tcatacccag cagtcaggca
     5881 atgagaaaag aggtacgatg tgatgaaaaa atatcaaacg tacatcgcaa taggttcact
     5941 attgagtttg atggttgtat taattacgta tggtttaatg caagacacgc aacatttgaa
     6001 cccacttgag tcacctaatg gacaacattg gttgggtacc gatcaattag gcagagactt
     6061 cttagtaaga ctgattgtcg gtagtcttgt cacattgagt ttaacaggca tagtgattct
     6121 attaagcgtt tgtatgggac ttatctttgg cttaattgca ggcatagaaa gacgatggtt
     6181 agatcaaatc atcatgtttg ttgccgatat gttgctggct attccgtcat ttattatcgc
     6241 attagtcatc ttaagtttag taagtaactc catgataggt ttgatacttg ctttaacgat
     6301 tggatggata ggacgttatt tacgttactt cagaaattta acgcgagata ttcaaaaacg
     6361 tccatttgtt caatatgcac gattgagtgg gaactcaaca ttcaaaacga cagtaacaca
     6421 tgtgattcca catttattaa gtagtatatt cgctttggta acggctgact ttggcaaaat
     6481 gatgctcagc atatctggac ttgcttttct aggactaggt attaaaccgc cgacgcctga
     6541 gttaggaaca attctttttg atgggaaaag ttatttcaac ggcgcaccgt ggctcttctt
     6601 cttccctggt gtattgttag gaggtttcgc cttattatgt caaattatca acaaaaaaat
     6661 aacgcagtaa atacggtagt caacgtcaat caattatcga ttttagatca agagaaagta
     6721 ttgttaaagg atgttgattt gacagtaact aaaggtgcat ttcattgcat tataggtgaa
     6781 agtggcagtg ggaaatcact gttaacaaga acaatacttg gaatgaaaca atcacaatta
     6841 tgttatcaag gagatattga catcgattta actcaaacag atgcagtgtt tcaagatgtt
     6901 caaagtaata tgtttcaaaa tataacatta gctaagcatt tccaatacat ttatgaagcc
     6961 aatcgcacac atctcactaa acagcgtatt aaggaagatg tcttacagat gatgcaatta
     7021 cttggtttaa gacaagggga acaattgctt gagcgttatc ccttcgaact tagtggaggt
     7081 atggcacaac gtgtcgcctt tataatgtca ttaattagac gtccgaacta cttattttta
     7141 gatgaaccaa cgagtgcact tgatcaagaa aatattaaaa agtttatgca ttaccttctt
     7201 agggcacagg agcgctacca aatgaccatt gtttttatca cacatgatat taacttagtg
     7261 aaagattgtg ccacacatat tagtattatg cagcaaggta aattgataga aaatggtgag
     7321 gccgcgtcga tcttaactaa gccgacacat aattacacga aaaaattaat tacgattgca
     7381 catcggagac aaccttatgc ttaaaataga gagattaacc aaatatatag acacgcaact
     7441 gatatttaaa gagatatcat gtacaattaa cgaccagcac ttactcataa gtggggagag
     7501 tggttgtggt aaatccacat tagccaagat tatcgctggc ttagatacgg attatcaggg
     7561 cgaattatat cttaatgggc gcttacgtga atcttatacg tctaaagagt ggatgaagca
     7621 catccaatat gtacctcaat atcaacgtga tactttaaat cagcgtaaaa cggtattagc
     7681 tacattatta gaaccactta agaattataa ggtaaataaa cagcgttata catcaagcat
     7741 tgaagcagtg cttgatcagt gtaatttacc acacgatata cttaatcata aagtttcgac
     7801 attaagtggt ggccaatttc aacgcgtctg gatagctaaa gctttaatat tagaaccaga
     7861 gattctcata ttggatgaag ctacaaccaa cttagatgtc attaatgaag aagctatact
     7921 tcaaatgttg atttccttaa agatgacaca attaatcatt atttcacatg atacatacgt
     7981 cttaagccaa tttgaaggaa ttcagttaca gctaaataaa ttgaataatt aagatcacaa
     8041 atcttaatat ggtgaatatt taatggtacc taaaaaataa aatttaaact acaatgtcta
     8101 aatccatatg ttgtttcatt agaggattta aaaatgatta
//
LOCUS       DAKTIP010000117         7937 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAKTIP010000117 DAKTIP010000000
VERSION     DAKTIP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.117"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            408..809
                     /locus_tag="O0305_001873"
     CDS             408..809
                     /locus_tag="O0305_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0275014.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(1072..2769)
                     /locus_tag="O0305_001874"
     CDS             complement(1072..2769)
                     /locus_tag="O0305_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCY0275015.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFITS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLYKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            3044..3667
                     /gene="gmk"
                     /locus_tag="O0305_001875"
     CDS             3044..3667
                     /gene="gmk"
                     /locus_tag="O0305_001875"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCY0275016.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            3667..3885
                     /locus_tag="O0305_001876"
     CDS             3667..3885
                     /locus_tag="O0305_001876"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCY0275017.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            4101..5300
                     /gene="coaBC"
                     /locus_tag="O0305_001877"
     CDS             4101..5300
                     /gene="coaBC"
                     /locus_tag="O0305_001877"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCY0275018.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            5300..7708
                     /gene="priA"
                     /locus_tag="O0305_001878"
     CDS             5300..7708
                     /gene="priA"
                     /locus_tag="O0305_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCY0275019.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         2765 a         1157 c         1443 g         2572 t
ORIGIN      
        1 ggggctgctt aaccgcatcc gaatattaca aacactatag cttttattat ataaaaaatt
       61 tattagcgga taattaactt tattaaaatc caaccaatta agaatacgag taataggact
      121 aatactggaa tcacataatg taacataacg tccctccttt aacttaattt taattgtaat
      181 caaatttgac aataagtcaa aacattaata cctatgataa gtatcattta ttaacatatg
      241 tatcatattt ttaatcttgc gtaattttta tcgttaacta tgtgatttaa tcaacaggcc
      301 aataagacgt ttgtcattcg tgcacataac ggtacgacgc gcttttgtca ttttaattat
      361 gttaagataa tagtagttat agaagttcaa tctataggag gcatagcatg gatattccaa
      421 aaatcacgac atttttaatg tttaataacc aagctgaaga agctgttaaa ctatacacaa
      481 gcttatttga agatagtgag attataacaa tggctaagta tggtgaaaat ggacctggtg
      541 atcccgggac tgtacaacac tcaatattta cattaaatgg acaagtattc atggcgattg
      601 atgctaatag tggcacagaa ttaccaatga atcctgcgat ttcattattt gttacagtaa
      661 aagatactat tgaaatggaa cgactattta atggattaaa agatgaaggt gccattttaa
      721 tgccaaaaac gaatatgcca ccatacagag agtttgcttg ggttcaagat aagtttggag
      781 taagttttca attagcatta cctgagtaaa aggattcgca cagcgttgaa tgataaagaa
      841 acactttttc ttatgcatgc gttttaccta tgtatttcta ttttaagtac acattagcat
      901 tttagtttca ctcattttta aaatcacaaa gataatttgt aacttaaatg atgatacgtt
      961 atttaaaaac acgatacttc gtttcaatga acgcattaaa taataaataa acacctcacc
     1021 ataagaaagg actactttct tttgtgaggt gtttatttgt cgacaacttg attatgattg
     1081 tttcattttt tgaataagtt cataatcagg tgtagcatac aaagtttttt gattgtcata
     1141 tgttacaaac ccaggctttg cacctgatgg tttatgcaca tttttaatta atgtataatc
     1201 aacaggtatt tgtccagaat taccagcttt tgaaaagtat cctgctaaca tagccgcttc
     1261 cttgattgtc gtatcacttg gtgcatcatt aaatataacg acatgtgaac caggaatatc
     1321 ttttgtgtgt aaccatgtgt gagttttttt agctttttta tttgttaaat aatcattttg
     1381 cttgttattc ttaccaacat atatatcgtc gccatcagtt gatacataat gttgtaattg
     1441 aatctgcgct ttctttttct tagtttgatt tttacgctgt ttcataaagc cttgttctgc
     1501 taattcatct ctaatttcat caatgtcatg gacagaaata tgatgtaatt gttgttcgat
     1561 tgttgaaaaa taatctatat tgtctttcgt caattgaatt tgatgttgta attcacgttc
     1621 tctcgttttc atacgattat attgtttata ataatattga gcatttgctg atggggattt
     1681 tgtaggattt aaaggaatga caacttcttc attcgtataa taattcaatg ccgtcacttc
     1741 tttatcgcct tgcttaattc gatatatatt agcagtgatc aattcaccat ataactgttc
     1801 agtatcttta tttttagact gctcatattc ttcaatcaac ttcgctaatt tattttgata
     1861 tttgtacaac tgctgttgaa caaatcgaac taaatcattc gcacgttgtt taacgcgttc
     1921 acgttcgcca cgcgcatcat aaaaacgatc aagcaaatca tttaatgaat cgtatgtaac
     1981 tgtatcatca ttaaattgat ttaactttat aaaatagaaa tcctctttac ctgtttcatg
     2041 atttttatga aaaataggag taggtggtaa cttggtttct gccattactt cgtcaaatgc
     2101 ttctggtaat gttgatgaag taataaattg acgacgacta acgatttcat tcgtaattaa
     2161 agggctaaat ccttcaaact gattcaataa ttgtttagca atattacctg cgttaaaatc
     2221 gatatatttc aacacctctg cacctgtaat atcatacgga tttattttgt gctgagtagg
     2281 tggtgcttca taattaaatc ctggcattac tgtacgatag tgattcgtat ttggtgttaa
     2341 gtgtttaaat ccttcaatta ttttgcgatt ttcatctact aaaattaagt tactatgttt
     2401 acccataatc tcaaggatga cagtgcggta aatagtatcg ccaatttcat ctttactctt
     2461 tatatcgatt tcaatgcgac gatcattacc aatttgctta atcgattcga taataccacc
     2521 ttctaagtgt tttctaaaaa cacgcgcaaa catgggtgga ttaaatggat tatcatattt
     2581 tttagtagtc aattgtaatc ttgaaaagtt tggatggatt gacaataaca attgatggtt
     2641 ttgtctattt tgacgtacaa ccattagtat cgtgtcatta tcaggttgat tgattttgtg
     2701 aacgcgtcct gttgttaaaa attgtagaga ctcaaccatt ttctttgtaa ataagccatc
     2761 ataagccata atttatcttc caccttcttc atatcattcg tttcatttta acatgattgt
     2821 aacttttcag tatagttatt gaaccataca ttatgccata cattttacat tatctaaaag
     2881 tttgcaattc aatttattta attaaaatta atcgtgcaat ataacgatat tgttagactt
     2941 aatagaaatt atggcatgca atttcaaata tgctatacaa tataaagaac aatgtgatat
     3001 catatttaaa taatagaaga ttagcttaga gaggtcgtaa ggcatggata atgaaaaagg
     3061 attgttaatc gttttatcag gaccatctgg agtaggtaaa ggtactgtta gaaaacgaat
     3121 atttgaagat ccaagtacat catataagta ttctatttca atgacaacac gtcaaatgcg
     3181 tgaaggtgaa gttgatggcg tagattactt ttttaaaact agggatgcgt ttgaagcttt
     3241 aatcaaagat gaccaattta tagaatatgc tgaatatgta ggcaactatt atggtacacc
     3301 agttcaatat gttaaagata caatggacga aggtcatgat gtatttttag aaattgaagt
     3361 agaaggtgca aagcaagtta gaaagaaatt tccagatgcg ctatttattt tcttagcacc
     3421 tccaagttta gaacacttga gagagcgatt agtaggtaga ggaacagaat ctgatgagaa
     3481 aatacaaagt cgtattaacg aagcgcgtaa agaagttgaa atgatgaatt tatacgatta
     3541 cgttgtagtt aatgatgaag tagaacttgc gaagaataga attcaatgta ttgtagaagc
     3601 tgagcactta aaaagagagc gcgtagaagc taagtataga aaaatgattt tggaggctaa
     3661 aaaataatgt taaatccacc tttaaaccaa ttaacgtcac aaattaaatc aaagtattta
     3721 attgcaacaa ctgcagcgaa aagagcgcgt gaaattgatg aacaacctga aactgaatta
     3781 ttaagtgaat atcattcatt taaaccagtt ggtagagcgt tagaagaaat tgctgacggt
     3841 aaaattcgcc ctgttatttc aagtgattat tatggtaaag aatagtttcg accattaaaa
     3901 atatgtgaag ctagacgtac atcaaatacg tcaaggcata tccaaatcaa acgcacctat
     3961 gtaggtgtgt ttttttagtt tatccaatga ttgatgttat aataatacta aatttgtatc
     4021 tataaaaaag taatgagcat ttgtgcgcat atgatgatgt aaagcgtaaa tgtcatagta
     4081 atatttaagg gagattgaat atgaagaaaa tattattagc cgttacaggt ggcattgcgg
     4141 catataaagc aattgatttg acaagtaagt taacacaatc tgggtatgaa gttcgcgtta
     4201 tgttaacgaa tcacgcacaa aaatttgtga caccattagc atttcaagca ataagtcgaa
     4261 atgctgttta tacagatact tttatagaag aaaatccttc agaaatacag catattgcat
     4321 taggtgattg ggcagatgca atcattgttg cacctgcaac ggcaaataca attgcaaaat
     4381 tgagtgtagg tattgctgat gatttggtga catcaacgtt gctagcaaca gagacaccga
     4441 aatttattgc gcctgctatg aatgtgcata tgtatgaaaa taaacgtacg cagcaaaata
     4501 ttaatatttt aaaagaagat gggtatcatt ttatcgaacc aggaagcgga tttctagcat
     4561 gtggttatgt tgctaaagga cgtatggaag aaccgcttca aatcgtttct gtaattgatg
     4621 ctcattttca aaatagtaat cgtttagcta atagttcatt tcaagataaa cgcgcattgg
     4681 ttacagcagg accaactatt gaagttatcg atccagtcag atttgtatcc aatcgttctt
     4741 ctggaaaaat gggctatgca atagctgaag cattgcgaaa tcgaggagct atcgtgacgt
     4801 tagttgctgg tcctacaaca ctagaggatc caaaagatat tgaagttatt catgttcaaa
     4861 gtgctgaaga aatgtttgaa caagtgacaa gccgatttga cgaacaagat attgttgtaa
     4921 aagcagcagc cgtatctgac tatacaccgg ttgatgtatt agaacataag atgaaaaagc
     4981 aggatggtga tttgtcagta tcttttaaac gtactaaaga cattcttaaa tatttaggtg
     5041 aacataaaac atcacagtat ttaataggct ttgcagcaga gactgaagat attgaaaatt
     5101 atgcacaaca aaaattacgc aagaaaaatg cagatgtgat tatttcaaat aatgttgggg
     5161 atatgtctat cggatttagt tctgatgata atgaattgac aatgcatttt aaaaataatg
     5221 aaaaagtaaa tatcaagaaa ggaaaaaaag tagtattagc tgcacaaatt ttagatgaac
     5281 tagaaactag gtggcaataa tgatagcgaa agtcatagtc gatgtcgcgt cgaagagcgt
     5341 tgactataaa tttgattata taattcccga acaactcgaa tctgtcatcc aacctggtgt
     5401 gcgtgtaatt gtaccttttg gaccaagaac gattcaaggt tatgtaatgg aagtaacagc
     5461 agaacctgat gcacaacttg acgtttcgaa gttaaaaaaa atcatagaag tgaaagatat
     5521 acaaccagaa ttaacatcag aattaatagc tttaagtgag tggatgggtt caactcatgt
     5581 cattaaacgt atttctatgc tagaagtgat gcttccgagt gctattaaag cgaagtataa
     5641 aaaagcattt aagatgaaag atgacataga gctaccttca gctttattac aaaaatttga
     5701 taagcatggt tactattatt ataaagatgc gcaaaaaaat aatgatattc aattgcttat
     5761 gaagttgtta aaagatgata tcgttgaaga aaaaacgatt ctcacacaaa atataactaa
     5821 aaaaaccaag cgtgctgttc gtgtcattga agggtatcat cctgatgaag tattagctaa
     5881 gttggagaaa gttattaaac aatacgattt gtatgcttac ttgtctgaag aacaacataa
     5941 aacaatattt ttaactgata ttgaggatat gggcttttca aaatccagtt tagatggact
     6001 tatcaaaaaa ggttatgttg aaaaatatga cgcggttgtt gaaagagacc catttaaaga
     6061 tcgtgttttc gaacaagaat caaaacagca attaacagaa gaccaatata aagcatatga
     6121 agcgattaaa gctaaaattg taagccaaga gcaagaaaca tttttacttc atggtgtgac
     6181 gggatcaggt aaaacagaag tatatttaca aacgatagaa gatgttttaa gccaaggaaa
     6241 acaggcgatg atgttagttc ctgaaatcgc tctaacaccg caaatggttt tacgcttcaa
     6301 acgtcgattt ggtgatgacg ttgctgtatt acattctggc ttatctaatg gggaacgtta
     6361 tgatgaatgg caaaaaatta gggatggtcg tgcgagagta agtgttggtg caaggtcaag
     6421 tgtgttcgca cctttcaaaa atttagggtt aatcatcatt gatgaagaac atgaatctac
     6481 atataaacaa gaagattatc cgagatatca cgctagagaa attgcccaat ggcgaagtga
     6541 atatcatcac tgtccagtca ttttaggaag tgcaacacca tgtcttgaaa gttatgcacg
     6601 agctgaaaaa ggcgtttatc atttgctatc attaccaaac agagtgaacc aacaagcttt
     6661 acctgaaatt gatatagtag acatgcgtga agaattgagt gaaggtaatc ggtcaatgtt
     6721 ttcaaaagat ttacgtgaag ccatacaatt aagattagat cgacaggaac aagttgtttt
     6781 atttttaaat cgacgtggtt atgcatcgtt tatgttatgt cgggattgtg gatatgtacc
     6841 gcaatgtcca aactgtgata tttcattaac gtatcataaa acgacagact tattaaaatg
     6901 tcactattgt ggttaccaag agacgccacc gaatcaatgt ccaaattgtg agagtgaaca
     6961 cattcgacaa gtaggtactg gtactcagaa agttgaagaa ctattgcaac aagaatttga
     7021 agatgcgcgc ataattagga tggatgtaga tacaacctca aagaaaggtg cacatgaaaa
     7081 gttattgact gaattcgaaa aaggtaacgg tgacatttta ctaggtactc agatgattgc
     7141 gaaaggatta gattatccaa atattacttt agttggtgtg ctgaatgcag atacaatgtt
     7201 aaatttacct gattttcggg cgagcgaacg tacttatcaa ctattaacgc aagtggctgg
     7261 tagagctggt cgtcatgaaa aggcaggtca agtcatcatt caaacgtata atccagatca
     7321 ttattcaata ttggatgttc aaaaaaatga ttatttaaca ttttatcgtc aggaaatgga
     7381 atatcgtaaa ttaggaaagt atccaccgta ttattatttg attaatttca caatctcaca
     7441 taaagaaatg aagaaggtta tggtagcatc gcagcatgtt cataaaattt tattacagca
     7501 tttaacagaa aaagcgcttg tactaggtcc atctccggca gcacttgcga gaatcaacaa
     7561 tgaatttaga ttccaaattt tagtgaaata taaaagtgaa cctggattat tacaagccat
     7621 tcagttttta gatgactatt accatgaaaa atttataaaa gaaaaattag cattgaagat
     7681 tgatattgat ccacagatga tgatgtaaca ttactaatta ttagaaacaa gttcagtatg
     7741 tacgagtatt tgaaccagtg tttgaatatt tactttaagt acagaaaaag ggcagaatat
     7801 acaactgtta actatttaaa ttagcagttt atattctgtc tttttatatg gctttataac
     7861 ttacgtgatt ttggtttgat aaggaattta ttagtatttt catttacgaa ttctgatttt
     7921 aatgcaagcg ccatact
//
LOCUS       DAKTIP010000118         7875 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.118, whole genome shotgun sequence.
ACCESSION   DAKTIP010000118 DAKTIP010000000
VERSION     DAKTIP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.118"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            12..554
                     /locus_tag="O0305_001879"
     CDS             12..554
                     /locus_tag="O0305_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCY0275020.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            659..1108
                     /locus_tag="O0305_001880"
     CDS             659..1108
                     /locus_tag="O0305_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0275021.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            1157..2044
                     /gene="xerD"
                     /locus_tag="O0305_001881"
     CDS             1157..2044
                     /gene="xerD"
                     /locus_tag="O0305_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCY0275022.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTVTEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(2122..2628)
                     /locus_tag="O0305_001882"
     CDS             complement(2122..2628)
                     /locus_tag="O0305_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCY0275023.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            2720..3451
                     /locus_tag="O0305_001883"
     CDS             2720..3451
                     /locus_tag="O0305_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCY0275024.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            3444..3986
                     /gene="scpB"
                     /locus_tag="O0305_001884"
     CDS             3444..3986
                     /gene="scpB"
                     /locus_tag="O0305_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCY0275025.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGMTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            3979..4716
                     /locus_tag="O0305_001885"
     CDS             3979..4716
                     /locus_tag="O0305_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCY0275026.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            4849..5574
                     /gene="srrA"
                     /locus_tag="O0305_001886"
     CDS             4849..5574
                     /gene="srrA"
                     /locus_tag="O0305_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCY0275027.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            5555..7306
                     /gene="srrB"
                     /locus_tag="O0305_001887"
     CDS             5555..7306
                     /gene="srrB"
                     /locus_tag="O0305_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCY0275028.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            7551..>7875
                     /locus_tag="O0305_001888"
     CDS             7551..>7875
                     /locus_tag="O0305_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0275029.1"
                     /translation="MKKFIGSVLATTLILGGCSTMENESKKDTKTETKSVPEEMEASK
                     YVGQGFQPPAEKNAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGI"
BASE COUNT         2911 a         1058 c         1498 g         2408 t
ORIGIN      
        1 acgtgattat aatggattta aatgaaaaaa caattgatcg aacagttatt tataatggca
       61 aaattgtaga tgtagaaatt catacagtga cattaccaaa cggtgaaacg tcaacaagag
      121 aattagttta tcataatggt gcagttgctg tttgtgcagt gacacctaaa aaagaagtcg
      181 tattagtgaa acagtatcgt aaaccagtag aaaaaccatt gctagaaatt ccagctggta
      241 aattagaaga tgatgaagat agagtcgaag cggcaaaacg tgaattagaa gaagaaacag
      301 gatatattgc caaagagtta acacatgttg tagatatgta tggttcacca ggtttttgcg
      361 atgaacaatt atcaatatat tttacggata atttagaaga aggtacagtt cacttagatg
      421 aagatgaatt tgtcgaagtc attaaagttc cgattgagaa tgttaaatcg atgctaatga
      481 ataaagaaat agaagatgcg aagactatca ttgcattgca acacctatta ttaaattata
      541 atcattctaa ataacaaatg agaaaagctt gcattttatt gagaaaactg ttagttttaa
      601 ttgtgaagtt tgaaataatt tgtaatgatt ttaattatta gtaggggagt ggacatcgtt
      661 ggaagaacga ttaaatcgcg ttaagcaaca attacaacaa tcatcatata agctaacgcc
      721 acaacgcgaa gctactgtta gagttctaat tgaaaatgaa aaagatcatc taagtgctga
      781 agacgtatat ctgaaagtaa aagataaagc gcctgaaatt ggcttggcga cagtatacag
      841 aacgttagag ttgttagctg aactaaaagt tgtcgacaaa attaactttg gtgatggcgt
      901 cgctcgtttt gatttaagaa aagaaggcgc aaaacatttc caccatcatt tagtatgtat
      961 ggaatgtggt cgtgtagatg aaatcgatga agatttgtta ccagaagttg aaaatcgagt
     1021 tgaaaatgag ttcaatttta aaattttaga tcatcgttta actttccatg gtgtgtgtga
     1081 aacgtgccaa gctaaaggta aaggatagta aattgcgtag gttaaattaa ccttcgcttt
     1141 ttttagaggt gtggttatgg aaacaattat tgaagaatat ttgcgtttta tacaaattga
     1201 aaaaggacta agttctaaca caattggtgc ttatagacgt gatttgaaaa agtatcaaga
     1261 ttatatgact gaacatcata tctcgcatat tgattttata gatcgacaat taattcaaga
     1321 gtgtttgggg catttaatag accaagggca atctgctaaa tctattgcgc gatttatttc
     1381 aacaatccgt agttttcatc aatttgctat aagagaaaaa tatgcggcga aagatccaac
     1441 ggtattatta gattcaccaa aatatgacaa aaaattgcct gacgttttaa atgttgacga
     1501 agtgttggct ttattagaaa cgccagattt aaataaaatt aatggatatc gtgatcgtac
     1561 gatgttagaa cttctgtacg caacgggaat gcgtgtatct gaattgatac atttagagtt
     1621 agaaaacgtg aacttaataa tgggatttgt acgcgtattt ggtaaaggcg ataaagaaag
     1681 aattgtacca ttaggcgacg cagtcattga gtacttaact acttatattg aaacgattag
     1741 accgcaactt ttaaaaaaga ctgttactga agtcttattt ttaaatatgc atggtaaacc
     1801 tttatcacga caagcaatat ggaaaatgat taaacaaaat ggtgtaaagg caaacattaa
     1861 aaagacgtta acgccacata cgttacgcca ctcttttgcg acacatttat tggaaaatgg
     1921 cgcagattta agagcagtgc aagagatgtt aggtcactct gacatatcta ctacccaact
     1981 ctatacacat gtttcgaaat ctcaaattag aaaaatgtat aaccaatttc atcctagagc
     2041 ataaagtgaa caataactca aaagtcacaa tacacatgac taaaaatgtc tgtgctattg
     2101 tggctttttt aaattggttg attaattacg tctatgtttt cttaattgaa tcgcttcttc
     2161 ttttgctgca atcacttctg aacgatcacg gcgcatgtga tggtctacaa taaaaggatc
     2221 tgttgctgtt tcctgattat aatcatagtc tggatagttg gccttgatga tgcgttcaaa
     2281 gactggagtt attggtaata taacagatga aaaaggcttt gctgcattca attttgcaat
     2341 ctgttgctca attaacaact gataatcatt taaattaagg tataacgcat ctctatcttt
     2401 agcattttgt attatttctt tagatttatt aaaagactta taggcgcctt ttaaattatt
     2461 gcggcgataa tggtaacaag cagttgcaaa caagattaaa ctaacaactg catcttgctt
     2521 actgtagtta ttttcagctt tccatgcatc ttctaaaatg tcatgacata ggaaataatg
     2581 ttgcttagta tgaaattgat aatagaaatt tatcagtgcc tgttgcattt tgttatcacc
     2641 ccaatttaaa agtaagttat tttcatgcta taatatttta gagaattatg cacatatgac
     2701 gcaatacgag gtagatatta tgtatgaagt taaattagat gctttcaatg gaccattaga
     2761 tttattgctg catcttatcc aaaaatttga aatagatatt tatgatattc ctatgcaagc
     2821 attaacagag cagtatatgc agtacgttca tgcaatgaaa cagcttgaaa ttaatattgc
     2881 aagtgaatac ctagtattag cgtcagaact cttaatgatt aaaagtaaga tgctattacc
     2941 acaatcaaca tcagatatgg atgttgatga tgacccacgg gaagatttag ttgggcgttt
     3001 aatagaatat caaaattata aagaatatac tgctatttta aatgacatga aagaagaaag
     3061 agatttttat tttacaaaaa gaccgacaga tttatctcat ttggaaacag atgaatcttg
     3121 ggatccaaat catacgattg atttaactga attaattgta gcttatcaaa gagttaaaaa
     3181 tagagttgag ttaaatacac ctaaatctgt tgaaatccga aaagagacat ttaccattca
     3241 acaagctaca gaacaagtga catcgagatt gaaagataaa gatcatttta acttctttag
     3301 tctgtttacg ttttctgagc caattgaaca agtagtcact cactttttag ctattttaga
     3361 gatgtcaaaa gcaggaataa ttaatattga gcagcaacgt aattttgaag atattaacat
     3421 tattagagga gtgaactacc attttggata atcatggtat attagagtcg cttttattta
     3481 cagctggcga tgaaggttta gatgaaaaac aactattaga aatattagat atgtcgaaag
     3541 accaactcgt tgaattaatt gaaaattatt catcacatgg attaatgata caacgatttg
     3601 gaatgacgta tgttttaacg actaaaaaag aagcggcaac gtatattgaa caattaattg
     3661 aacaaaagtc acaaatgaaa ttatcacaag cagcaatgga agtactatca attattgctt
     3721 ataaccagcc attatcaaga agtgatattg aattaattcg tagtatcaat tcagatggtg
     3781 cagttaagac attgattgcc aaaggactag ttgaggctaa agtggttaat gaacagcgta
     3841 gccaacagtt aattactact gatttatttt taaatgtatt tggtatttcc aatatagaag
     3901 atttgccgac aactgaagaa gacgatgaag aaatggatgc ttttttcagt aatctagtca
     3961 atcaaaaagg agaaaataat gactaaagaa ttagaacgat tacaaaaacg tatagctaat
     4021 agcggttata cttcaagacg taaggcagaa actttaattt ctgaaggaaa agtgaaagtg
     4081 aatggtacga ctgtcactga attaggaaca aaagtgaagc catcagatac gattgaagtt
     4141 gaaggtatta aaatagaact agaagacaaa atttacattt tattccataa accaactcaa
     4201 gtgataacaa gtgtatctga tgatagagga cgtacagtgg ttacagatta ctttaaagat
     4261 atagaagcac gtatttatcc tgttggtcgt ttagactatg atacttctgg attgttatta
     4321 ctcacaaatg atggtgaatt tactaattta atgacacatc caagatatca aattaagaaa
     4381 aaatatgttg cgaaattaaa aggttattta atgagagaag aagtgaaagc gctagaaaaa
     4441 ggtattgaat tagaagatgg tatgacgcaa ccggctgaag tgaaagttaa gaaacaagat
     4501 aaagataaaa acacaacatt ggttgaaatt acaattacag aaggtcgtaa tcgccaagtc
     4561 agaagaatgt tcgaacattt tggtcatcaa gtgacgaaac tgtctcgtat tgaatatggg
     4621 cctttaaatg ttgtcggttt gaatgctggt gaaggacgtg tattgacgcc tcatgaagta
     4681 aaagtgatgc gtcatttagc agaacatggg aaataattaa ataaaatatg tatttatcac
     4741 aaagtttgag aattttttca caaaatttta ggtttttaat aactgtatgc gctttcctgt
     4801 gatataatac ttttgtaaag aaaagcatgt gtgggaggta tgacctgtat gtcgaacgaa
     4861 atacttatcg tagatgatga ggatagaatc agaagattac ttaaaatgta tttagaaaga
     4921 gaatcttttg aaatccatga agcaagtaat ggccaagagg cttatgaact tgcaatggag
     4981 aataattatg cttgcatact actagattta atgttgcctg aaatggatgg tatccaggtg
     5041 gcaactaaat tgcgtgaaca taaacaaaca ccgattatta tgttgactgc taaaggtgaa
     5101 gaaacaaacc gtgttgaagg ttttgaatct ggtgcagatg attatatcgt caaaccattt
     5161 tcaccaagag aagtagtctt aagagttaaa gcacttctaa gaagaacgca atctacaact
     5221 gtagaacaaa gcgaacctca cgcacgtgat gtgattgaat ttaaacattt agaaatagat
     5281 aatgatgcac atcgcgtact tgctgataat caagaagtta atttgactcc taaagagtac
     5341 gaattattaa tatatttagc taaaacacca aataaagtat ttgaccgtga acaattatta
     5401 aaagaagttt ggcattatga attctatggt gatttaagaa cagttgatac tcatgttaaa
     5461 cgacttagag aaaagttaaa tcgtgtgtct agtgaagctg cgcatatgat tcaaacagtc
     5521 tggggcgttg ggtataaatt tgaggttaaa tctaatgatg agccggctaa atagtgtcgt
     5581 aattaaactg tggttaacta ttattttaat agtgacgaca gttttaattt tattaagtat
     5641 tgctttaatt acctttatgc aatactattt cacacaagaa accgaaaatg ccataagaga
     5701 agatgctaga cgtataagtt cactggtcga acaatcacat aataaagaag aagcaataaa
     5761 atatagtcaa acattaattg aaaatcctgg tgggttgatg attataaata ataaacatcg
     5821 tcaatcaacg gcttcacttt ctaatattaa aaagcaaatg ttgaatgaag tagtcaacaa
     5881 cgaccatttt gacgatgtgt ttgataaagg taaatctgtt actcgaaatg taacgattaa
     5941 agaaaagggc tcatctcaaa catatatttt gttaggctat ccaacaaaag cacagaagaa
     6001 tagtcatagc aaatatagtg gagtctttat atataaagac ttgaaatcaa tcgaagatac
     6061 aaataatgct attacgatta tcaccataat tacggctgtt attttcttaa caattacaac
     6121 agtctttgcg tttttcttat cgtcaagaat tacaaaacct ttaagacgtt taagagacca
     6181 agctacacgt gtatctgaag gggattactc ttataaacct tctgtcacaa cgaaagatga
     6241 aattggtcaa ttatcgcagg catttaatca gatgagtaca gaaatcgaag agcatgtcga
     6301 cgcattatcc acatctaaaa atattagaga cagcttaatt aactctatgg tagaaggtgt
     6361 cctaggtatt aatgagagtc gacaaattat cttatctaat aagatggcga atgatattat
     6421 ggacaatatt gatgaagatg ctaaagcttt cttattaaga caaatagaag atacttttaa
     6481 atcaaaacaa actgaaatgc gcgatttaga aatgaatgca cgattctttg ttgtgaccac
     6541 aagctatatc gacaagattg aacagggagg taaaagtggt gttgttgtga cagttcgtga
     6601 tatgactaat gagcacaatc tagatcaaat gaagaaagat ttcattgcta atgtatcaca
     6661 tgaattacgt acaccgatat cattacttca aggttatact gaatcaattg tagatggtat
     6721 tgttacagaa ccggatgaaa taaaagaatc gcttgccatt gtccttgatg aatcgaaacg
     6781 tttaaatcgt ttagttaatg aattgttaaa tgtcgcacgc atggatgctg aagggttatc
     6841 cgtaaataaa gaagttcagc ctattgcagc gttactagat aagatgaaaa ttaagtatcg
     6901 ccaacaagct gatgatttag gtctaaatat gacttttaat tattgtaaga agcgtgtttg
     6961 gagttatgat atggatcgca tggaccaagt actaacgaac ttaattgata atgcatcacg
     7021 ttatacgaaa cctggagatg aaattgcaat tacttgtgat gaaaatgaaa gcgaagatat
     7081 tttatacatt aaagatacag gtacaggcat tgcaccagaa catttacaac aagtatttga
     7141 tcgtttttat aaagttgatg cagcgagaac gcgaggtaaa caaggtaccg gtttaggttt
     7201 gttcatttgt aaaatgatta tcgaagagca tggtggttcc atagatgtta aaagcgaatt
     7261 agggaaaggc acaacattta ttattaaact accaaaacca gaataaaatt gaatatagtt
     7321 atttcagaac gcatgttatt gattagagac tctaatttat agcatgcgtt ttttgattga
     7381 tgtggggaat tttgtatgtg gattagaact tagggttttt gcgaatatca actattaaat
     7441 atattactaa tttatacaaa aatataaagt ttgataaagt tatttatttg attataaaaa
     7501 tagggtaaaa tatagatata ttgtattaat taaattattc gaggtgtcat atgaaaaaat
     7561 tcattggatc agttttagct acgacattaa ttttaggggg atgttccacg atggaaaatg
     7621 aatcaaaaaa agacacaaaa acagaaacaa aatctgtacc agaagaaatg gaagcttcaa
     7681 aatatgtagg ccaaggcttt caaccgcctg cagaaaaaaa tgcgattgaa tttgcgaaga
     7741 agcatcgtaa agaatttgaa aaagtaggtg aacaattctt taaagataac tttggactaa
     7801 aagttaaagc tacaaatgtt gtaggtaaag atgatggtgt agaagtttat gtgcattgtg
     7861 aagatcatgg cattg
//
LOCUS       DAKTIP010000119         7800 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAKTIP010000119 DAKTIP010000000
VERSION     DAKTIP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.119"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(86..892)
                     /gene="yjbH"
                     /locus_tag="O0305_001889"
     CDS             complement(86..892)
                     /gene="yjbH"
                     /locus_tag="O0305_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCY0275030.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
     gene            complement(915..1280)
                     /locus_tag="O0305_001890"
     CDS             complement(915..1280)
                     /locus_tag="O0305_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCY0275031.1"
                     /translation="MTTTPYDIIGKEALYDMIHYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHIDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            complement(1384..1977)
                     /locus_tag="O0305_001891"
     CDS             complement(1384..1977)
                     /locus_tag="O0305_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCY0275032.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            2163..2510
                     /locus_tag="O0305_001892"
     CDS             2163..2510
                     /locus_tag="O0305_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275033.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            2527..3162
                     /locus_tag="O0305_001893"
     CDS             2527..3162
                     /locus_tag="O0305_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCY0275034.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            3179..3988
                     /locus_tag="O0305_001894"
     CDS             3179..3988
                     /locus_tag="O0305_001894"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCY0275035.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            3985..4839
                     /locus_tag="O0305_001895"
     CDS             3985..4839
                     /locus_tag="O0305_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0275036.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDRIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            4860..6245
                     /gene="mgtE"
                     /locus_tag="O0305_001896"
     CDS             4860..6245
                     /gene="mgtE"
                     /locus_tag="O0305_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCY0275037.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            6255..>7800
                     /locus_tag="O0305_001897"
     CDS             6255..>7800
                     /locus_tag="O0305_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCY0275038.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFS"
BASE COUNT         2615 a         1102 c         1396 g         2687 t
ORIGIN      
        1 tacgttctaa taatttaatg ttgcaaaatt aagagtcagt aaaaagacct cgttacattt
       61 atggtgtacg aggtctttaa tttatttatt ttgatttgat tttaggcatt ttagatttcc
      121 agaaatcgcc atctggatat ttaagttttt caattttttg ttgaatggct aacttcttta
      181 actctttgtt taaaagtttt tctggccatt cataaatagt aagtaattct tccatcgtta
      241 caagttgttg ttgctgtata taagtttcta atttaggagg aagattcttt tcgataggtt
      301 tacccatcaa ttcattaatt atataagtat agatgtgata tgggtataat ccttcgactt
      361 ttaaaccttc ttcatgaaca tcttcactaa agaaaacgag agaaggggct tgttcgattt
      421 ccatttctct tgcaatatgc aaatcaattt tcaagctttc ggttagttta cttttttgta
      481 agtcgtcttt aaatacttct aaatcaatac ctgcattttg aatacagtca caaatcattg
      541 attctgtaat aatatctctt ttaggtatga tttcattttg cattaaatgt ataaatcgtt
      601 cggcacgtac acgaccttgt aactcagctg ctttataagc tagggcgatg ttatcaaagt
      661 tggatgtact ttgagcttgg catttcgtta atacttttaa cgaaggattt aatatatgtc
      721 tgatacgtat atattgatta tattcaattc ttaatttgga taagattgct gataatttga
      781 agcaatcgga gctaaatgga tcgaaaaatg aataaatttc gattttactt acaggtgata
      841 gattgatatc ttcacgactc ttattttcca ttattcgtaa ttctccagcc atgtttattc
      901 acctacaatt aattttagga attcaccata tgattagcag ttaatcttaa gcgctcaaat
      961 aaataatctc caacaccttg tggaaacgca gcgcgattaa ttgctgtctg catattttct
     1021 aaccatgcat ctctttcaaa ttcagtgatt gtaaaatcta tatgtctttt tcttagcata
     1081 ggatgtccgt gttcttcggt ataaatgttt ggaccgccca aaaactgtgt taaaaattgt
     1141 ttttgtttac gacttgtttc tgcaaaatct cctggaaaca ggtgattaag tcgttcatct
     1201 ttttctacaa gggtgtaaaa ataatgaatc atatcgtata acgcttcttt accaatgatg
     1261 tcatatggtg ttgttgtcat ttaatcaccc attttcaaaa atttactgtt acgaacttaa
     1321 gttaatatat aactaatata acatgatttt aaacatttga aagaaatatg catatttgcc
     1381 aatttaattt atattgtttg aaagtgtttc ttttttcttg aaaaaacgtt gaactttatt
     1441 taaaggttga tgatgttcga ggtttagttc gtttaataaa gattggaact tttgtaaacc
     1501 ttgattatag tctttaactt cgaactctaa ctcataatcc gtagtatcga aatactcact
     1561 tttatctaaa accagtaaat cacctttata tttagtttct tggcgatatg tcgttaatgc
     1621 accaagtatt gataaagttg tatcttttac accaaactgt tcaactataa tttgacgaat
     1681 gtcatctgga agattgtcgt ttgaaataat caagttcatc tctggtttaa tgtcgacgat
     1741 atagttgtat tctaatagac caacctttgc tggtgtcttt aaagtcattt catattgatt
     1801 gtctttaact cttatgcgta gtgcagagcg atgttccttt aatttgaaat cgggtgtatc
     1861 aatatagtaa ttgacttgct taaaaagcac actgtcttta aaatatttct cttgcaattt
     1921 attatagatt gatgcagtta tcatttgttt aaattctatt tcatgatttg ttgccatgat
     1981 atgtatacac ctcgtatcaa attcaattta tcttaactat attatgaatg acaaagttga
     2041 attttaaaag taatttcctt tatctattat caatgttaat ttgaccatta aaaatagtgt
     2101 tcgtaagtgt tttgtattat tgaattgtgt taaaatgtta tggaataaga ggaggattaa
     2161 gcatgcgttt atatattaat gaaattaaaa ttaaagatga catactttat tgttatacag
     2221 aagattctat taaaggatta tctgaagtag gacaaatgct cgttgatagt gataattatg
     2281 cctttgcgta tacattagat gatggtaaag cgtatgctta tctcattttc gtacaagaaa
     2341 catggacgat gttgcatgaa aacatgacta aaaaaattat tatcaatgat gaactagaat
     2401 tgactgaatt ccaccaagaa cttacttata ttttagacaa cataaaaggg aataataatt
     2461 atggtaagga atttgttgca accgttgaag aaacattcga cattgaataa agcggggtga
     2521 agcactatga atcaatggga tcagttctta acaccttata agcaagcggt tgatgagttg
     2581 aaagtgaaac ttaaaggcat gcgcaaacaa tatgaagttg gtgaacaagc gtcgccaata
     2641 gaatttgtta ctggtcgtgt taaaccaatc gctagtatta tagataaggc aaacaaacga
     2701 caaataccat ttgataggtt aagagaagaa atgtacgata tcgctggttt aagaatgatg
     2761 tgccaatttg ttgaagatat tgatgttgtc gtcaatattt taagacaaag aaaagatttt
     2821 aaagtaattg aagaacgaga ttatattcgt aacactaaag aaagtggtta ccgctcgtat
     2881 catgtcatta ttgaatatcc aattgaaaca ttacaaggcc aaaaatttat attggctgag
     2941 attcagattc gtacattagc aatgaatttc tgggcaacga ttgaacatac tttacgatat
     3001 aaatatgatg gtgcttatcc ggatgaaatt caacatcgtt tggaaagagc ggcagaagca
     3061 gcgtatttac ttgatgaaga gatgtctgaa attaaagatg aaattcagga agctcaaaaa
     3121 tattacacgc aaaaacgttc taaaaaacat gaaaatgatt aacgaggtgt tataaatcat
     3181 gcgttataca attttaacta aaggtgactc caagtctaat gccttaaagc ataaaatgat
     3241 gaactatatg aaagattttc gcatgattga ggatagtgaa aatcctgaaa ttgttatttc
     3301 agttggtggt gacggtacat tactacaagc attccatcag tatagccaca tgttatcaaa
     3361 agtggcattt gttggagttc atacaggtca tttaggattt tatgcggatt ggttacctca
     3421 tgaagttgaa aaattaatca tcgaaattaa taattcagag tttcaggtca ttgaatatcc
     3481 attgcttgaa attattatga gatacaacga caacggctat gaaacaaggt atttagcatt
     3541 aaatgaagca acgatgaaaa ctgaaaatgg ctcaacactt gttgtggatg ttaacttaag
     3601 agggaaacac tttgagcgat ttagaggcga tggattatgt gtatcaacac cttcgggttc
     3661 aacggcttat aacaaagcgc taggtggcgc actgatacat ccttcacttg aagcaatgca
     3721 aattacagaa attgcctcga taaataatcg tgtgtttaga acggtaggat caccacttgt
     3781 attaccaaag catcatacat gtttaatatc accagttaat catgatacca ttagaatgac
     3841 gatagatcat gttagtatca aacataaaaa tgttaattca atacaatacc gtgtagcaaa
     3901 tgaaaaagtg aggtttgcac gttttagacc attcccattc tggaaacgtg tgcacgattc
     3961 tttcatatca agtgatgaag aacgatgaaa tttaagtatc atatatcaca acaagaaact
     4021 gttaaaactt ttttagcacg acatgatttt tctaagaaga cagtgagcgc cattaaaaat
     4081 aatggcgctt taattgttaa tgatgaacca gtcacagtgc gtaagcaatt aatgacaaat
     4141 gatatattag aaattcattt accgcgagaa ataccgagtg ttaatttaat accttatgct
     4201 cgtaagctag aagtattgta tgaagatgct tttatcatca tagttactaa accaaacaat
     4261 caaaattgta cgccttcgag agaacatcct catgaaagtt taatcgaaca agtactatat
     4321 cattgtcagg aacatggtga aaatattaac ccacatattg ttacgcgtct agatcgtaat
     4381 acaactggta ttgtgatatt cgctaaatat ggacatatcc atcatttatt ttctaaagta
     4441 aacttgaaaa aaatatatac ttgccttgta tatggtaaaa cccatacatc tggtattatt
     4501 gaagctaata ttagacggtc aaaggatagg attataacta gagaagttgc ctcggatggt
     4561 aaatacgcta aaacatctta tgaagtaata aatcagaatg ataaatacag tttatgcaaa
     4621 gttcatttgc atacgggacg tacacatcaa attcgtgtac attttcaaca tattgggcat
     4681 ccaattgtgg gagattcttt gtatgatggt tttcatgaca aaattcatgg tcaagtactg
     4741 caatgtacgc aaatatattt tgttcatcca atcaataaga acaatattta tattacaatt
     4801 gattataagc aattacttaa attattcaat caactctaat tcacacaggg ggtgtaagta
     4861 tgtcaatgaa cacagatgaa aaagagcgtg ttcaagagga attatacgac caaactttat
     4921 tagatcaata tttagaaaat gatgatattg atcaatttag agatgaattt ctagcattac
     4981 acacatatga acaaagtgag tattttgaag atactaccga tgaaaataga caaaagattt
     5041 ttcaatattt atcacctgaa gaagttgcaa atttctttga tcaattagat attgatgacg
     5101 atgaatatga gttgctattt gataagatga atgcgacata cgcaagtcac atattagaag
     5161 aaatgtcata cgacaatgca gtagatattt taaatgagtt gactaaacca aaagttgcta
     5221 gtcttttaac attgatgaat aaagatgacg cgaatgaaat caaagcatta cttcactatg
     5281 atgaggatac ggccggcggt attatgacga cggagtattt atcacttaaa gcgcatacgc
     5341 ctgttaaaga agcattatta ttggtcaaag cgcaagcacc agacgcagaa acaatatatg
     5401 ttatatttgt cgttgatgat gatggtaaat tagtaggtgt tttatcgcta agagatttaa
     5461 ttgtagctga aaatgatgct tatattgaag atattatgaa tgaacgtgtc attagtgtga
     5521 atgtagcaga cgaccaagaa gatgttgctc aagttatgag agactatgat ttcatggctg
     5581 tacctgttat agattaccaa gaacatttgc ttggtatcat cacgattgat gatattttag
     5641 acgttatgga tgaagaggct agtgaagact actctcgttt agccggggta tcagatatcg
     5701 attcgactaa tgattcaatc attaaaacag cattaaaacg tttaccatgg ttgattattt
     5761 taacattttt aggaatgatt actgcgacaa ttttagggag attcgaaaaa acattagaaa
     5821 atgtagcgct actcgcagcg tttattccta ttattagtgg tatgtcagga aattcaggta
     5881 cacaatcttt agccgtttca gttcgtaaca ttacgacagg ggaaattaat gagcaaagta
     5941 aatttagaat tgcattaaga gaagcaggaa gtggtgtatt atcgggtgtt gtatgttcaa
     6001 caatattatt tacaattatt gttgcaatat atcatcagcc acttttagca ttaatcgttg
     6061 caggaagttt aacttgtgcg atgacggtgg ggacgtttgt aggttcgatg attccattat
     6121 tgatgaataa attaaatatc gatccagcag tggctagtgg accatttatt acaacaatta
     6181 atgatattat tagtatgttg atttattttg gtttagctac atcatttatg gcttacttaa
     6241 tttaaggagg agttatggag tttttatctt tagttattgt tgttttagca gcgtttttaa
     6301 ctccaataat tgtcaatcga ttaaatatta atttcttgcc agttgttgtt gcagaaattt
     6361 tgatggggat tgtgattgga aattcatttc taaatatagt agaaagggat tcaattctaa
     6421 atattttatc aacgttaggc tttatctttt taatgttttt aagtggttta gaaattgatt
     6481 ttaaagcttt taaaaaagat aaacgcgcac gtcaaggaca aaatgatgat gaatcctcaa
     6541 ttccagggca tcttaatcta gcgttaactg tatttgcatt tattatgatt atttcgattc
     6601 ttttagcgta tgtatttaaa tggcttggat tagtggatga tgtgttatta atggtcatta
     6661 tcatttcaac tatttcctta ggcgtagttg ttccaacttt aaaagaaatg aatattatga
     6721 gaacaactat agggcaattt atcctattag tagcagtact tgcggactta gtaactatga
     6781 ttttattaac ggtctatggc gcaatcaatg gtcaaggcgg cagtacaata tggttaatag
     6841 gtatattagt tgttttcaca gcaatttcat atattttagg tgttcaattt aaaagaatgt
     6901 catttttaca aaaattgatg gatggtacga cgcaaatcgg tattcgtgcg gtatttgcat
     6961 taataatatt attagtagcc ctagcagagg gagttggcgc agaaaatata ttaggtgcat
     7021 tcttagcagg tgtcgttgtt tcattattaa atccagatga agaaatggtt gaaaagttag
     7081 actcatttgg ttatgggttc tttattccta ttttctttat aatggttggt gtagatttaa
     7141 acataccttc attaattaaa gaaccgaaat tactaattat cataccgatt ttaatcgttg
     7201 catttatcat ttcaaaatta attccagtca tgtttattcg acgttggttt gatatgaaaa
     7261 caacgattgc atcagcattt ttattaacat caacattatc gctcgtgata gctgcagcca
     7321 aaatttcaga aagattaaat gctatttcag ctgaaacgtc aggtatatta attttaagcg
     7381 cagtcattac atgtgtattc gttccgatta ttttcaaaaa actgtttcca gttccagatg
     7441 agtttaaccg taaaattgaa gttagtttaa ttggtaaaaa tcaattaacg attcctatag
     7501 cgcaaaattt aacatctcag ttatatgacg tgacattata ttatcgcaaa gacttgagtg
     7561 atcgtcgtca attgtcagat gatatcacga tgatagaaat tgctgattat gaacaagatg
     7621 ttttagaacg actaggtctg tttgaccgag acatagttgt ttgtgctacg aatgacgatg
     7681 atattaaccg aaaagttgct aaattagcca aagcacatca agttgagcgt gtcatttgca
     7741 gacttgaaag cacaacggac gatacagagt tagttgattc aggtattgaa attttcagta
//
LOCUS       DAKTIP010000120         7775 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAKTIP010000120 DAKTIP010000000
VERSION     DAKTIP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.120"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..462)
                     /locus_tag="O0305_001898"
     CDS             complement(<1..462)
                     /locus_tag="O0305_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HCY0275039.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEET"
     gene            complement(586..720)
                     /locus_tag="O0305_001899"
     CDS             complement(586..720)
                     /locus_tag="O0305_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0275040.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            complement(777..911)
                     /locus_tag="O0305_001900"
     CDS             complement(777..911)
                     /locus_tag="O0305_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCY0275041.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1275..1348
                     /locus_tag="O0305_001901"
     tRNA            1275..1348
                     /locus_tag="O0305_001901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1309..1311,aa:Arg,seq:tct)
     gene            complement(1823..2011)
                     /locus_tag="O0305_001902"
     CDS             complement(1823..2011)
                     /locus_tag="O0305_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0275042.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            2260..3207
                     /locus_tag="O0305_001903"
     CDS             2260..3207
                     /locus_tag="O0305_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCY0275043.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLKNVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKEKQDKFLRNASQ"
     gene            complement(3512..3739)
                     /locus_tag="O0305_001904"
     CDS             complement(3512..3739)
                     /locus_tag="O0305_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275044.1"
                     /translation="MTHLTKVLDTLTGICVVLLFRKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4047..5603)
                     /locus_tag="O0305_001905"
     CDS             complement(4047..5603)
                     /locus_tag="O0305_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCY0275045.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            complement(5775..6707)
                     /gene="arcC"
                     /locus_tag="O0305_001906"
     CDS             complement(5775..6707)
                     /gene="arcC"
                     /locus_tag="O0305_001906"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0275046.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            complement(6730..7731)
                     /gene="argF"
                     /locus_tag="O0305_001907"
     CDS             complement(6730..7731)
                     /gene="argF"
                     /locus_tag="O0305_001907"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0275047.1"
                     /translation="MKNLRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLK
                     NKNIALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYD
                     GIEYRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTY
                     VGDGRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDI
                     DQGVKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEIMDKTGNPNVIFEHCLPS
                     FHNADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
BASE COUNT         2501 a         1447 c         1043 g         2784 t
ORIGIN      
        1 tgtttcttca gatataaata tctttttaat atatttatgc aacggcgtct gatttaacct
       61 tctcttttgc gtttcggtta caccattagt aacaatatat aaatcatgtc ttttcgataa
      121 ttcgacaatt gtttctaatg tttgatcaaa gtatttaact ttagcttctg ctaatccatt
      181 tctaaataac acatctgcac gatgcccatc aacttccatt tgatgatgtt tgaagtaatt
      241 cacaaatcgt tctgataata cttcagactt cgttaattta ttttgttgaa aagcttccca
      301 atgttggtga ttgatttttt taaatgttaa aaaatcatcc tttgttgctt tatgattaaa
      361 aacattcgcc atatagtgaa acgcccattc ttctgcatca taaaaatcaa caattgtatc
      421 atcaaagtct atcaaaatat ttttatatcc caatttcccc atctcctata ttgtctatgt
      481 atctaaatct taacagaggc tcaaatttct gcaaataaaa taaactgagt gcataacatt
      541 aaagtatgct cacccagttt attttaaaga atattagtta ttatattaga atccaaataa
      601 tttacctagt aaacccacac cgttagcaac gatgtctacg atacttgtgc ctaatttcac
      661 actatcatgt tgttgtgcag cttgcacagt atttgcgatt gcttctgcta gtccagtcat
      721 ttaaatctct ccctcacctt tgaaataata ctgattactt acataacata ttgaaattag
      781 aatccgaata atttacctaa taaacctacg ccattttcaa cgatgctcac aatgcttgtg
      841 cctaattttg cgccatcatt attaattgct gcagttacgg tatctttaat tgcgttaaat
      901 aaaccttcca ttgaaaacac tccttaaaat ttaaatttga agataacaaa aacgtgcgta
      961 gcttttaaat caccgaaatg ttattcgctt atcgttttgt tgttgttatt ttaaaataaa
     1021 tttgatacaa ttagtttgtt tatccgcaca acatcttata atgtacttaa ctgtatttta
     1081 aagagaaaag aaatacagtt aggcattcaa aactgtattt aacacaatta agttgcctga
     1141 attcgtattt aagtcttatt gaaccttttt agataaatag ctctataata gtgaaaaata
     1201 taaacatttt ttatttacaa ggtattgcta atttaagttc atttagatat aataattctt
     1261 gtgttgttaa acgtgtcctg gtagctcagc tggatagagc aatggccttc taagccatcg
     1321 gtcgggggtt cgaatccctc ccaggacgtt tataggtatt tttatacgca ttaccaaaca
     1381 aaagagttcc gtgattacgg ggctcttttt gttttgaatt tcagtaatat agtatgatgc
     1441 gtcaccaaaa cgtcccccgc ataagccccg aaaatacagt aattaaaaca agcatgctta
     1501 ttcgttatag aattttttga cacacaattg acacgcgtct gacacttgtt tatacatttt
     1561 taattaagta attttgtgct caaatttcat ctatactgca cctgaactac accaacacta
     1621 caccaagatt tttaacactc accatttgca tgcgtagaga tttttattat tatattattc
     1681 ctatagattt tgatactatt caaaatttta gggacttttc aggggcccga aatcctataa
     1741 ttataattat atacatctaa aaaaaataac cacgtccatc gagacgtggt tttttaatta
     1801 tttaactcac taattattct tcttaatttt cattgctaaa tatactattg gcaaatttat
     1861 aaagtagttc agcgtttttc aatgacatat tgtctaatga tctttcattt tttctcattc
     1921 tgtgtattgt gctttgtgga actcctgttt gtttcgatat atgtaaactg ctcaaatcac
     1981 tgtctaatag tttttgaatt tgatttctca ttttgtacac ccccttgtaa atcttcaatc
     2041 atcatttaaa ttaaataatt acttttccac atattccaat atttaggttg caaagcatac
     2101 ctcaaatatc attaattttg agatttaaat gtcaattatg tgtctatcaa tccaatatac
     2161 atactctaat aacgtaatag tacacactct tcttattaat tggccatagc tatcatgata
     2221 taattagtga agaaaatcac ataagaaagg ttgtaaatca tgagacttca aaaagcacct
     2281 ctagtaacgt caggactagt cttaggatta ttaggcctgg gtaatctatt aaaagactta
     2341 tctcttactt taaacgctgt ttgcggaatc tttgctttct tgatttggat tcacctttta
     2401 tgtactatga tcaaatattt taataatgtg aaagaacaat taaacagtcc tctagtttca
     2461 tcagtgttca caacattttt catgtctggc tttttaggta ctacttattt aaatacattt
     2521 tttagtaaca taacttttat caatagctta ataacgccta tttggatttt atgccttgtg
     2581 ggaattatga cgcatatgat tattttttca ataaaatatt taaaagattt ttcacttaaa
     2641 aatgtttatc cttcgtggac tgtacttttt attggtattg ctatcgcagg attgacggca
     2701 cccgttagcg gatatttttt cataggtcaa ttaacagtaa tatatggctt tgtagctact
     2761 tgtattgtct tacctatagt tttcaagcga ttaaaagcat ttccattgca gacgtcaatc
     2821 aaaccgaaca catcgacaat ttgtgcacca ttttctttag tcgctgcagc atatgttata
     2881 gcttttccta aggcgaatgc ttttatcgta attatatttt tactattagc tcaaatattt
     2941 tatttttata tcattataca attgcctaaa ttactaaaag aacctttttc gcccgtattt
     3001 tcagctttca cattcccttt agtaatctca gcaactgctt taaagaacag tttgcctgta
     3061 cttatgtttc cagacatttg gaaaggtctt ttgtttatcg aagtgttatt agccactgta
     3121 atagtactta gagtctttat aggatatctt cacttctttt taaaaaagga aaaacaagat
     3181 aaatttcttc gtaatgcgtc tcagtaacac tattaccaag aattaacacg tatatttaat
     3241 aactaaaaag aggcaggtaa tcttgcctct ttttagtgta tatcaatgcc tctttgattt
     3301 aatatgatct tatcaatgat accttctgac taaagctata gtaactatac tagaacgtct
     3361 agttcataaa cacgtttcac tctaaacttt atttaaataa tatataagca gcaaacgcca
     3421 aggctaatat accaataaaa taattaataa agatttttat tttagtcttt gtaatagttg
     3481 acaaatttta tcacctctaa atatcgataa attaaacacc gttatttttc ctttgcctat
     3541 cttttatcat ttcagaaggt actccgaaaa taaacatcag aataaataat ataattggca
     3601 aatgtggtat gttttctagc aaataccatc ttaaattcca atcaaacacc atatttgcat
     3661 acgccacaaa atattttcta aataataata ctacgcatat tccagttagt gtatctaaaa
     3721 cctttgtcaa atgtgtcatt tatagccatc ctttatatat gctattttca caaccatatg
     3781 tatcatgtgt tacaaaaact gtttacagca aatacatatt taccacgata aatataatat
     3841 accatagatc taacattaca ttaaaaacaa attttaaaaa tatcattatt aaatagagca
     3901 ttgtatcaat tacttactag tgttatcatt accgtcatta ttttcttaat caaaaataat
     3961 aatgcacctc gcttagttta gtatttctac aaacctacga gatgcataaa accccattag
     4021 tattttttat aatatagcat ttcaaattat gaataaatta gtacttgcgt aattagtact
     4081 ccgccaccga aaatcaatac aaaagcaact accggccata caaatcggaa ccaatgtgaa
     4141 tatcgcatgt ttaacatttg tagtgtggcc ataacaagtc cagtcggcgc taagaataac
     4201 atcgcatatt gaccaaattg atatgtcgta acgatgacga atcttggtat acctactgta
     4261 tcagctagtg gtgcaaagat aggcattgat aataccgcta agccagaaga tgatggcacg
     4321 ataaaaccta aacagaagaa aataaatagt aatacgatga taaataatgg tccactcata
     4381 tgttgaacta aagatgatga aaagtgtaag attgtatctg aaatcatacc ttcattcaac
     4441 actaaattaa tacctcgagc taaaccaata attaaagata cacctactaa acttgatgca
     4501 ccattgacaa atgcatctac agttcctttt tctcccaatc cagatttacc tgtcccagca
     4561 ataaacatta ttataattgt aaatattaaa aatgctgaag ccataactgg gaaccaccaa
     4621 ccttgcgtca taactcccca taccataatt ggaaatggta gtacaaataa tgtaaggatt
     4681 atcttcttac gcaaagtaaa atgggcacta tcgtcatctt ttaatacaga ccattgctgt
     4741 tcaaaagcat ctttgtcttc ataagaatat gacgctttag gatcgttttt aattttttta
     4801 cagtaccaat ataaataact aataacaaaa atcgcaccga caatacaagc acctattctc
     4861 caatacaagc catccgtaaa agttgtacca gcggcattag aggcaattac aaccgagaac
     4921 gggttaatag ttgaaaatgt actaccgaca gagctggcaa ggaatatggc accaactgaa
     4981 acgatagaat cgtatcctaa cgctataaat atagggacta aaatcggata aaatgctaca
     5041 gcttcttctt caataccaca taatgtcccg ccgataatca ttagtattga tacaaacaca
     5101 attagcataa attcatgccc ttttgttttc ttcgttaaag ctaacaatcc cgattcaaaa
     5161 gaaccactgg cttgaactac gccaataagt ccccctagta caagaatgaa taccatgata
     5221 tcgaccgctt ctatcgtacc ttcaaccatg ctacttgtta tttgttctgg tccagctgga
     5281 tgttgcttta atctttcata agtattcgga attgataccg gcttattaat tgcacctgat
     5341 ttaaattgtt caatcttaat tttaaccccc attttgtcta gttcctgttg cgtacccgga
     5401 acctttttca cttggttatg agggttaact atctttagtt cttgggatga aggttcgtaa
     5461 gaaagtttag aatatgcacc agcaggaata acccatgttg ctataactgc aacaaccgtt
     5521 aaaatgaata aaatcataaa tgcacctggc atttttaatt taaatcgttt tttcttttca
     5581 ctttcattaa ttgtattttc cacggtttca tctccttcga catttaacct agcatttcta
     5641 ccttaaagat tttataaata taaattaaga aagtgcaccc cgcatcaaaa tagaggcatt
     5701 attttcaggg ggtgcacata aataataaaa atcatgcatt tgacatatag taattgaaaa
     5761 gcgtttcaat tcaattactt tttaatcaca gtacctactt taccctctaa ggcagcatct
     5821 aattcattta atgatgttat aagcacactt ccttttggat tgttttcaat aaatgatatg
     5881 gctgcttcaa tttttggtaa catacttcct tttgcaaatt gattttcgtc tatatatcgt
     5941 tttaattcat caacatttgt tgttttcaaa ggctgttggt tttcagtgtt aaaattaata
     6001 tatacataat caattgctgt taaaataatc aattgatcgc attgaatatt agcacccaac
     6061 aacgcacttg ttttatcttt gtctataact gcatcaatac ctttaaaacc atcatgttgc
     6121 tctctaatta ctggtatacc tccaccacca gcagcaataa cgagtgtatc atttttaata
     6181 agtgttttaa tactctctaa ttcaataata gagatgggtt gtggtgaagg aacaacgcgt
     6241 ctatatcctc ttccagcatc ttcaacaaat ataaatcctt tttctttttg aatttgttca
     6301 gcttcttctt tgttgtaaaa taacccaatt ggttttgaag gattgttaaa tgccggatca
     6361 ttttcatcaa cttcaacttg tgtcactagt gttaccactt gtttatccat tccaatagaa
     6421 tgcaattcat tttgtaagct ttcttgtaat tgatagccga tgtaagcttg actcattgcg
     6481 ccacattcag caaatggaaa tgccggacct tggttatgtt ctgcagcata gttaagtccc
     6541 aaattaatgc ttccaacctg tggtccatta ccatgactaa taacaatctc atgtcctttt
     6601 gttattaatc ctactaatga tttcgcagta tttttaacaa gctcgagttg ttcttgaggt
     6661 gattttccta aagcattacc acctaatgct actactattt tcgccatcat attcacttcc
     6721 ttatatcatt taaaattcac ccaatgtagc aaccatgact gctttgattg tatgcattct
     6781 gttctcagct tcttggaata caactgaagc tttactttcg aatacttcat ctgtaacttc
     6841 catttctcga ataccatatt tttcaaaaat ttgttgacct attttcgtat cagcattatg
     6901 gaaagatggt aagcaatgct caaaaataac atttggatta ccagttttat ccattatttc
     6961 tttatttact tgatatggtt tcaataattc aagtcgttct ttccatactt catcaggttc
     7021 acccattgat acccaaacat cagtgtaaat tacatccgaa ccttttacac cttggtcaat
     7081 atcatctgtg attaatatgt tgccaccatt ttcagcggca atatttttac agcgatttaa
     7141 taattcatct gttggattta attcttttgg acaaactaaa tggaagttca tacccataat
     7201 ggcagcacct tgcattaatg catttgcaac gttattacga ccatctccaa catatgtaaa
     7261 gttaatatct gcataatctt tttttaagac ttcttttgct gttaagaaat cagcaagaac
     7321 ttgagtagga tgatcttcat cagttaaacc attccacact ggtacgcctg aatactcagc
     7381 taaagtttct actgttcttt gtgaaaaacc acggtattca atgccatcat acattccacc
     7441 aagcacacgt gcagtatctt tagttgtttc ttttttaccc atttgtgatc cagttgggcc
     7501 taaataagtt acatttgcac cttgatcatg cgctgcaact tcaaatgcac atcgcgttct
     7561 tgtagaatct ttttcaaata acagtgcaat atttttattt tttaacatag gcttttcagt
     7621 gccaatatat ttagcacgtt ttaaatcctc ggagagtgtt aataagaatt ctacctcttg
     7681 tcgtgaaaag tctaataaag ttaaaaaact tctgtttcgt aaatttttca ttaataacaa
     7741 ctccttttat tttagttaca cctaaattgt aagcg
//
LOCUS       DAKTIP010000121         7726 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAKTIP010000121 DAKTIP010000000
VERSION     DAKTIP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.121"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            208..2304
                     /gene="pnp"
                     /locus_tag="O0305_001908"
     CDS             208..2304
                     /gene="pnp"
                     /locus_tag="O0305_001908"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCY0275048.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2540..4213
                     /locus_tag="O0305_001909"
     CDS             2540..4213
                     /locus_tag="O0305_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCY0275049.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4469..6838
                     /locus_tag="O0305_001910"
     CDS             4469..6838
                     /locus_tag="O0305_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCY0275050.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6843..7556
                     /locus_tag="O0305_001911"
     CDS             6843..7556
                     /locus_tag="O0305_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0275051.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7587..>7726
                     /locus_tag="O0305_001912"
     CDS             7587..>7726
                     /locus_tag="O0305_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCY0275052.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KL"
BASE COUNT         2813 a         1072 c         1517 g         2324 t
ORIGIN      
        1 ataatcaaga tattaaaaat aaagtatgtt tttttaaaaa atataactta tatttatact
       61 gataagggtg ggacgataag tctattttgt aaataataga tggatatccc gctctctttt
      121 tttccaattc aatattttat aactaatatt aaaatacgat aataaatgat atgatataac
      181 tattagattc aagagaggag atttataatg tctcaagaaa agaaagtttt taaaactgaa
      241 tgggcaggaa gatctttaac gattgaaaca gggcaattag ctaaacaagc aaatggcgct
      301 gtattggttc gttatggaga tacagtcgtg ttatcgacgg caactgcatc aaaagaacct
      361 cgtgatggag atttcttccc attaacagtg aactatgaag aaaaaatgta cgctgcgggt
      421 aaaattcctg gtggatttaa aaagagagaa ggacgtcctg gtgacgatgc aacattaact
      481 gcgcgattaa ttgatagacc aattagacct ttattcccta aaggatataa gcatgatgtt
      541 caaattatga acatggtatt aagtgcagat cctgattgtt caccacaaat ggctgcaatg
      601 attggttcat ctatggcgct tagtgtgtcg gatattccat tccaagggcc aatcgccggt
      661 gtaaatgtgg gttatattga cggtaaatat atcattaacc caacagtaga agaaaaagaa
      721 gtttctcgtt tagaccttga agtagctggt cataaagatg cggtaaacat ggtagaggca
      781 ggcgctagtg agattactga acaagaaatg ttagaggcga ttttctttgg tcatgaagag
      841 attcaacgtt tagttgattt ccaacaacaa atcgtcgacc acattcaacc tgttaaacaa
      901 gaatttattc cagcagagcg tgatgaagcg ctagttgaac gtgtaaaatc tttaaccgaa
      961 gaaaaaggac ttaaagaaac agttttaaca tttgataaac aacaacgaga tgaaaatctt
     1021 gataacttaa aagaagaaat cgtcaatgaa tttatcgatg aagaagatcc agagaatgaa
     1081 ttacttatta aagaagttta tgcaatttta aatgaattag tgaaagaaga agttcgacgt
     1141 ttaattgcag atgaaaaaat tagaccagac ggccgtaaac ctgatgaaat ccgtccatta
     1201 gattctgaag ttggtatttt acctagaacg catggttcag gtctatttac acgtggtcag
     1261 actcaagcac tttcagtttt aacattaggt gctttaggcg attatcaatt aattgatggt
     1321 ttaggacctg aagaagaaaa aagattcatg catcattaca acttcccgaa tttttcagta
     1381 ggtgaaactg gtccagtacg tgcgccaggt cgtcgtgaaa ttggacatgg tgcgttaggt
     1441 gaaagagcat taaaatatat tattcctgat actgctgatt tcccatatac aattcgtatt
     1501 gtaagtgagg tacttgaatc aaatggttca tcatctcaag cgtcaatttg tggatcaaca
     1561 ttagcattaa tggatgcggg cgtaccgatt aaagcaccag ttgctggtat tgctatgggc
     1621 cttgttacac gtgaagatag ctatacgatt ttaactgata tccaaggtat ggaagatgca
     1681 ttaggtgata tggactttaa agtcgctggt actaaagaag gtattacagc aatccaaatg
     1741 gatattaaaa ttgacggttt aacgcgtgaa attatcgaag aggctctaga acaagcgaga
     1801 cgtggtcgtt tagaaataat gaatcatatg ttacaaacaa ttgatcaacc acgtactgaa
     1861 ttaagtgctt acgcgccaaa agttgtaact atgacaatta aaccagataa gattagagat
     1921 gttatcggac ctggtggtaa aaaaattaac gaaattattg atgaaacagg tgttaaatta
     1981 gatattgaac aagatggtac tatctttatt ggtgctgttg atcaagctat gataaatcgt
     2041 gctcgtgaaa tcattgagga aattacacgt gaagcggaag taggtcaaac ttatcaagcc
     2101 actgttaaac gtattgaaaa atacggtgcg tttgtaggcc tattcccagg taaagatgcg
     2161 ttgcttcaca tttcacaaat ttcaaaaaat agaattgaaa aagtggaaga tgtattaaaa
     2221 atcggtgaca caattgaagt taagattact gaaattgata aacaaggtcg agtaaatgct
     2281 tcacatagag cattagaaga ataatattta aagtcatatg acgacaatgt atcgtcatgt
     2341 gattttttta tgccactttt tacgaagtga cccgttttga atttgttgta ttgaacattt
     2401 taaaacgctt tattattttg tgtgcaactg ttaattatcc tgtatgtata gtgattaata
     2461 gtgtacatca agtgtttttt aacttataat gaatagtgag tttatatatg gacgggtaac
     2521 aaatttagga ggtaagattt tgagtttaat aaagaaaaag aataaagata ttcgcattat
     2581 accattaggc ggtgttggcg aaattgctaa aaatatgtat atcgttgaag tagacgatga
     2641 aatgtttatg ttagatgctg gacttatgtt tccagaagac gaaatgctag gtattgatat
     2701 tgttatacca gacatttcat acgtacttga aaataaagat aaattgaagg gtatattcct
     2761 tacacacgga catgagcacg cgattggtgc agtgagttat gttttagaac aattagatgc
     2821 accagtatat ggatctaaat tgacaatagc gttaattaaa gaaaatatga aagcccgtaa
     2881 tattgataaa aaagttcgct actatacagt taataatgat tcaattatga gattcaaaaa
     2941 cgtgaatatt agtttcttta atacgacaca cagtattcct gatagtttag gtgtttgtat
     3001 tcacacttca tatggtgcca ttgtgtatac aggtgaattt aagtttgacc aaagtttaca
     3061 tggacattat gcaccagata ttaaacgtat ggcagagatt ggtgaagaag gcgtatttgt
     3121 cttaatcagt gattctactg aggcagagaa acctggatat aatactccgg aaaatgtgat
     3181 tgaacatcat atgtatgatg cttttgcaaa agtgcgaggt cgcttgatag tttcatgtta
     3241 tgcttcgaac tttatacgta ttcagcaagt tttaaatatt gctagcaagc taaatcgtaa
     3301 agtgtcattt ttaggaagat cacttgaaag ttcatttaat attgctcgta aaatggggta
     3361 tttcgacatt cctaaagatt tgctaattcc tataacagaa gttgataatt atcctaaaaa
     3421 tgaagtgata attatagcta ctggtatgca aggagaacct gtagaagcct taagtcaaat
     3481 ggcgcaacat aagcataaaa ttatgaatat cgaagaaggc gattctgtat ttttagcaat
     3541 tacggcttct gctaatatgg aagttatcat tgcgaataca ttaaatgagc ttgtacgtgc
     3601 tggcgcacat attattccaa ataacaaaaa gattcatgct tcaagtcatg gttgcatgga
     3661 agaattaaaa atgatgatta atattatgaa acctgaatac tttattcctg tacaaggtga
     3721 atttaaaatg cagatagcac atgcgaagct agcagctgaa gcaggtgttg caccagaaaa
     3781 gattttcctt gtggaaaaag gagatgtcat taattacaac ggtaaagata tgatattaaa
     3841 tgaaaaggta aattcaggaa atattttaat agatggcatt ggtattgggg atgtaggaaa
     3901 tatcgtgttg agagaccgtc atcttttagc agaagatggt atctttattg ctgttgtaac
     3961 gttagatcct aaaaatagac gtatagctgc gggacctgaa attcaatctc gtgggtttgt
     4021 atatgtacgt gaaagtgaag acttattacg tgaagcagaa gagaaagtac gtgaaatagt
     4081 agaggctggt ttacaagaaa aacgcataga atggtctgaa attaaacaaa atatgcgtga
     4141 tcaaattagt aaactattat tcgaaagtac aaaacgtcgt cctatgatta ttccagtaat
     4201 ttctgaaatt taatcaaaaa gtcattaaca taaaagaggt cagaacaagt cactgaaata
     4261 taatggttgt catggacaat ttacttatat tttatgatgg tcaattgaag gggtaacgat
     4321 taatctgtta tcttaagtaa attgatacat agatgatatt gttctaacct ctttcatcgt
     4381 ctgtttggac tacatattct aaacatcaaa taggaaatta tatataataa cgtcgtttta
     4441 actaaggcaa cataaggagg tgcgtcaatt ggcacaagca aaaaagaaat cgacagctaa
     4501 gaaaaaaaca acatcaaaaa aaagaacaaa ttcgaggaaa aagaagaatg ataatccgat
     4561 acgttatgtc atagctattt tagtagttgt attaatggtg ttgggtgttt tccaattagg
     4621 aataataggt cgtctaattg acagcttctt taattattta tttgggtaca gtagatattt
     4681 aacatatatt ttagtactct tagcaactgg ttttattaca tactctaaac gtattcctaa
     4741 aactagacga acggctggtt cgattgtatt gcaaattgca ttgctatttg tatcacagtt
     4801 agtttttcat tttaatagtg gtatcaaagc tgaaagagaa cctgtacttt cttatgtgta
     4861 tcagtcatac caacacagtc atttcccaaa ttttggtggc ggtgtattag gcttttattt
     4921 attagagtta agcgtacctt taatttcatt atttggtgta tgtattatta ctattttatt
     4981 attatgctca agtgttattt tattaacaaa ccatcaacat cgtgaagttg caaaagttgc
     5041 actggaaaat ataaaagctt ggtttggttc atttaatgaa aaaatgtcgg aaagaaacca
     5101 agaaaaacaa ttgaagcgtg aagaaaaagc aagacttaaa gaagaacaaa aggcacgtca
     5161 aaatgaacag ccacaaataa aagatgtgag tgattttacg gaagtgcctc aagaaagaga
     5221 tattccaatt tatgggcata ctgaaaatga aagtaaaagc cagagtcaac caagtcgaaa
     5281 aaaacgagtg tttgatgcag agaatagttc gaataacatc gtaaatcatc atcaagcaga
     5341 tcagcaagaa caattaacag aacaaactca taacagtgtt gaaagtgaaa acactattga
     5401 agaagctggt gaagttacga atgtatcgta tgttgttcca ccgttaactt tacttaatca
     5461 acctgcaaaa caaaaagcaa catctaaagc tgaagtgcaa cgtaaaggac aagtactaga
     5521 gaatacatta aaagattttg gggtaaatgc aaaagtgaca caaattaaaa ttggtcctgc
     5581 agtaactcaa tatgaaattc aaccagctca aggggttaaa gtgagtaaaa ttgtaaactt
     5641 gcataatgat attgcattag ctttagcagc aaaagatgtt agaatcgaag cgccaatacc
     5701 tggtcgttct gcagtaggta ttgaagtgcc aaatgagaaa atttcattag tttcactaaa
     5761 agaagtttta gatgaaaaat tcccgtctaa taataaacta gaagttggat taggaagaga
     5821 tatatcaggt gatccaatta ctgttccact aaatgaaatg ccacacttat tggtggcagg
     5881 atcgacgggt agtggtaaat ctgtttgtat aaatggtatt attacaagta ttttattaaa
     5941 tgctaagccg catgaagtta aacttatgtt aatcgatccg aaaatggttg aactaaatgt
     6001 ttataacgga attccacact tattaattcc ggttgttaca aatcctcata aagctgctca
     6061 agctttagaa aaaattgtag ctgagatgga aagacgttat gatttattcc aacattcatc
     6121 aactagaaac attaaaggtt ataacgaatt aatccgtaag caaaatcaag aattagatga
     6181 gaagcaacca gaattacctt atatcgttgt tattgtagat gagcttgcag atttaatgat
     6241 ggtagctggt aaagaagttg aaaatgcgat tcaacgtatt acacaaatgg cacgtgcagc
     6301 aggtatacat ttaattgtag cgacacaaag accttctgtg gatgtaatta caggtatcat
     6361 taaaaataat attccatcta gaatagcttt tgctgtgagt tctcaaacag attcaagaac
     6421 tattattggt actggcggcg cagaaaagtt acttggtaaa ggtgacatgt tatacgttgg
     6481 aaatggtgac tcatcacaaa cacgtattca aggggcgttt ttaagtgacc aagaggtgca
     6541 agatgttgta aattatgtag tagaacaaca acaggcaaat tatgtaaaag aaatggaacc
     6601 agatgcacca gtggataaat cggaaatgaa aagtgaagat gctttatatg atgaagcgta
     6661 tttgtttgtt gttgaacaac aaaaggcaag tacatcattg ttacaacgcc aatttagaat
     6721 tggttataat agagcatcta ggttgatgga tgatttagaa cgcaatcagg taatcggtcc
     6781 acaaaaagga agcaagccta gacaagtttt aatagatctt aataatgacg aggtgtaaaa
     6841 aaatgtcaga aatgaatgcg gtatataacg ttaaacaata cattttaaat ttgattaagc
     6901 aaaataaatt ggaatatggt gaccaacttc caagtaattt atcaattgcc agagaattaa
     6961 atgtaaaaac cgacgatgtt tatgaagcaa ttcaggcatt gattactgaa caagtcatta
     7021 aagataattt tgaagagggc acaagtgtta agtcactgcc ccctttcttt tatccattga
     7081 atgaacttat aagtattggg caaatgatta aaaatgcagg atttgaatgc ggaactgaat
     7141 acttaaattt tgatgagcaa ccagcaacta tgttagatgc aaatttgttg agcgttgaag
     7201 aaggatatcc agtaaccatt atagaacgat tacgaactgc cgatggagaa ccggtcgtct
     7261 attgtttaga taaaattgct aaaaaagaat taacatgtac agagtatcaa atgagcaatg
     7321 gatcgattct aagtgcgata aaagaacaaa gtaatcataa tatttgttat gctgatacag
     7381 aaattgaagc ggtaaattat gaacctcgaa tatccgaagt actgaatgct tcgccacacg
     7441 aaggtttgat tttattaaaa attacgcact ataatgaatc agatgaacct attttgtatt
     7501 cattaaatta tatgaaaaat agcttagttc aatttaaaat cactagaaaa atatagatgt
     7561 aagaagatat agaggaggct atatatttga gtagacaatc tcaaccaaat atacatatca
     7621 aagtttcacc aacaactaaa tttaaaacaa ctactatagt ttttaaattt atggcacctt
     7681 tagaatatga cacaataaca gctagatcat tattaagcaa attatt
//
LOCUS       DAKTIP010000122         7674 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAKTIP010000122 DAKTIP010000000
VERSION     DAKTIP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.122"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..690)
                     /locus_tag="O0305_001913"
     CDS             complement(<1..690)
                     /locus_tag="O0305_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCY0275053.1"
                     /translation="MKFTVIAKAIFILGILTTSVMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGSADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVN"
     gene            complement(1034..1729)
                     /locus_tag="O0305_001914"
     CDS             complement(1034..1729)
                     /locus_tag="O0305_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCY0275054.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVQAKEKQERVQHLYDIK
                     DLHRYYSSESFEFSNISGKVENYNGSNVVRFNQENQNHQLFLLGKDKEKYKEGIEGKD
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFSINKEEV
                     SLKELDFKIRQHLVKNYGLYKGTTKYGKITINLKDGEKQEIDLGDKLQFERMGDVLNS
                     KDINKIEVTLKQI"
     gene            complement(2150..2845)
                     /locus_tag="O0305_001915"
     CDS             complement(2150..2845)
                     /locus_tag="O0305_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL6"
                     /protein_id="HCY0275055.1"
                     /translation="MKLKTLAKATLVLGLLATGVITTESQTVKAAESTQGQHNYKSLK
                     YYYSKPSIELKNLDGLYRQKVTDKGVYVWKDRKDYFVGLLGKDIEKYPQGEHDKQDAF
                     LVIEEETVNGRQYSIGGLSKTNSKEFSKEVDVKVTRKIDESSEKSKDSKFKITKEEIS
                     LKELDFKLRKKLMEEEKLYGAVNNRKGKIVVKMEDDKFYTFELTKKLQPHRMGDTIDG
                     TKIKEINVELEYK"
     gene            complement(3291..3995)
                     /locus_tag="O0305_001916"
     CDS             complement(3291..3995)
                     /locus_tag="O0305_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCY0275056.1"
                     /translation="MKMTAIAKASLALGILATGTITSLHQTVNASEHKAKYENVTKDI
                     FDLRDYYSGASKELKNVTGYRYSKGGKHYLIFDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVFSYGGVTKKNQDAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEISLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            complement(4359..5285)
                     /locus_tag="O0305_001917"
     CDS             complement(4359..5285)
                     /locus_tag="O0305_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCY0275057.1"
                     /translation="MKITTIAKTSLALGLLTTGVITTTTQAANATTPSSTKVEAPQST
                     PPSTKIEAPQSKPNATTPPSTKVEAPQQTANATTPPSTKVTTPPSTNTPQPMQSTKSD
                     TPQSPTTKQVPTEINPKFKDLRAYYTKPSLEFKNEIGIILKKWTTIRFMNVVPDYFIY
                     KIALVGKDDKKYGEGVHRNVDVFVVLEENNYNLEKYSVGGITKSNSKKVDHKAGVRIT
                     KEDNKGTISHDVSEFKITKEQISLKELDFKLRKQLIEKNNLYGNVGSGKIVIKMKNGG
                     KYTFELHKKLQENRMADVIDGTNIDNIEVNIK"
     gene            complement(5394..5531)
                     /locus_tag="O0305_001918"
     CDS             complement(5394..5531)
                     /locus_tag="O0305_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275058.1"
                     /translation="MFVKVAFLCLKSDETSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            complement(5650..6708)
                     /locus_tag="O0305_001919"
     CDS             complement(5650..6708)
                     /locus_tag="O0305_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCY0275059.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKIQSTKVDKVPTL
                     KAERLAMINITAGANSATTQAANTRQERTPKLEKAPNTNEEKTSASKIEKISQPKQEE
                     QKTLNISATPAPKQEQSQTTTESTTPKTKVTTPPSTNTPQPMQSTKSDTPQSPTIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVNHKVELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMAGTNIDNIEVNIK"
     gene            complement(6999..>7674)
                     /locus_tag="O0305_001920"
     CDS             complement(6999..>7674)
                     /locus_tag="O0305_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCY0275060.1"
                     /translation="KISLLLGILATGVNTTTEKPVHAEKKPIVISENSKKLKAYYNQP
                     SIEYKNVTGYISFIQPSIKFMNIIDGNSVNNIALIGKDKQHYHTGVHRNLNIFYVNED
                     KRFEGAKYSIGGITSANDKAVDLIAEARVIKEDHTGEYDYDFFPFKIDKEAMSLKEID
                     FKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINVTDIDRIE
                     IKVIKA"
BASE COUNT         2181 a         1355 c         1006 g         3132 t
ORIGIN      
        1 attcacttcg atgtttttaa tttgttcact attaatgaca tctgccatac gctcgaaatc
       61 taatttatca cttaaatcaa tttcatactt attttcatct ttcatattaa taacaattct
      121 acctttatca gctgaccctt catacagttt gtatttttta atcaacagtt ttcttatttt
      181 aaaatcaagt tccttcaatg acacttcttc cttttgaata aagaaaaatt catcaataga
      241 aaaaccgtct ttatcgtcta tttttttaac tagtaagttc ggcgttctta gagactcaaa
      301 tattgatttt acgtttttct ttgttacacc actaacacta aatattcttc catctaaatc
      361 tactaattct ggtaccgcaa agacatctaa accatgtgtt ttttctttgt atttattttc
      421 atcttttccc aataaaaaca cttggaactt ttgattttgt tggttaaaaa gcaaaacgtt
      481 agagtcataa taaccttgac tttggccact aacatttgtt aactcataac taggtcctga
      541 atagtattga tgtaacttgt ttgtatcata taaacggttc attttttcat actttccttt
      601 tgcattaacc gattgatttt ctgttatcat tacacttgtt gttaatattc ctaatataaa
      661 tatcgcttta gctatcactg taaatttcat attcatgtgc tccaatctta atatattgga
      721 ttgcttttat tacgtaattt gaataataca cctttattat aaagggcatt gagacagact
      781 gacattaaca ctcgtttaat taaacttaaa tcattgttaa tgaagagcta attaatattc
      841 aatgggcatc actttcaaga gcaatgtaaa tttttagtat gctcactcat aacctgcgtt
      901 ataaaacgta aaaaaaagcc taacgacaag gttgttttaa atacgctcct taatgtaagg
      961 tgtacgtatc tattcaacat ttcatcgtta agctgcttca aatttattac tttagagtca
     1021 ttgattactt actttaaatt tgtttcaaag tcacttcaat cttattaata tccttactat
     1081 tcaacacatc acccatgcgc tcgaattgca atttatcacc taaatcaatt tcttgctttt
     1141 ctccatcttt caaattgata gtgatcttac cgtatttagt cgtaccttta tataaaccat
     1201 aatttttaac taaatgttgt ctaattttga aatcaagttc tttcagtgaa acttcttctt
     1261 tattaattga aaatgagtca attgatgcat ctaaatttcc gccatacact ttattaacaa
     1321 ataaatgtgt attagtttca gaagatttgt tatttttctt agtcacacca ccaacagtag
     1381 ataatctacc gtttggatca attaattctt ttaccacaaa gacatctttg ccttcaatgc
     1441 cttctttata tttctcttta tctttaccta ataagaataa ttggtgattt tgattttctt
     1501 ggttaaagcg tacaacgtta gaaccgttat aattttcaac cttaccacta atattactga
     1561 attcaaaact ttctgatgag tagtatcgat gtaagtcttt aatatcatat aaatgttgta
     1621 ctctctcttg cttttctttt gcttgaactg cttggccttc tgatgtaatc acaccagtag
     1681 ttaataagcc taatgccaat gttgctttag ctaacgtttt taatttcaca gtactattct
     1741 cccaatctat ttataaattt gtcttaatat atttttatat gattaattca attacgtaat
     1801 acgaacaatc tactatcatt atataagtga gatactgcgc tatgaattaa cctcttttta
     1861 actatttttg aaccaacgtt aagttagtta atgaaatcct aacagtatta attatttttt
     1921 ataacacaat actaattttt taactattca cactttccct caatttaatt atgttttcca
     1981 cattgtttca tgtcacgaaa aggacaacgc gcgactataa gtatcaaccg tttttccaaa
     2041 aacttgatca taaacccgct ccttttttca tcataacaaa ataaaaaaga agctaagcaa
     2101 tatgttatcg cttaacttcc ctacaattac tagtctgctt gtccaaagat tatttatatt
     2161 ctagctcaac attaatttct ttgattttgg taccatctat cgtgtcaccc atgcgatgcg
     2221 gttgtagttt ttttgtaagt tcgaaagtat aaaacttatc atcttccatt ttaactacaa
     2281 ttttaccttt tctattatta acagcaccat ataatttttc ttcttccatc aatttttttc
     2341 ttaatttaaa gtccaactct tttaacgaga tttcttcttt agtaatttta aatttactat
     2401 ctttagactt ttccgatgat tcatcaattt ttcttgttac tttaacatcg acttctttac
     2461 taaattcttt actatttgtc ttacttaaac caccaattga atattgtctt ccattaacag
     2521 tttcctcctc gatgactaaa aatgcatctt gcttatcatg ctcaccttga gggtattttt
     2581 caatatcttt accaagcaag ccaacaaaat aatcttttcg atccttccaa acatatactc
     2641 ctttatctgt cactttctgt ctatacaaac catcaagatt ttttaactct atacttggct
     2701 tgctatagta gtattttaac gatttataat tgtgttgacc ttgagttgat tctgccgctt
     2761 ttactgtttg actttctgtt gttattacac cagtagctaa caatcccaat actaatgttg
     2821 ctttagctaa cgtttttaat ttcatagtac tattctccca atctatttat aaattttgtc
     2881 ttaatatatt tttatatgat tatttcaatt acgtaatacg aacaatctac tatcattata
     2941 taagcgtaat actaagctat gaattaacct ctttttaact atttttgaac caatgttaag
     3001 ctaattaatg gaatcctaac agcgttaatc tattttttaa acttaacgca gcttttttaa
     3061 ctattcacac tttccctcaa tttaactatg ttttccacat tgtttcatgt cacgaaaagg
     3121 acaacgcgcg actataagta tcaactattt ccacaagttt tattggtgtt tttattattc
     3181 atcgatacgc ttcattttca tctctccaac acaaaaaaga agctaagcaa cttatgttgc
     3241 ctaactcctc tatactatcc atattttact attatccata tttcattgaa ttatctaatg
     3301 ttggcttcta ttttttcaat atttctaccg tcaatgacgt cactcatgcg atttgtttgt
     3361 aattttttat taagttcaaa cgtataatag ccgccatctt tcattatcac ttttatctta
     3421 ctatctttag gaaacttttt atacagatca aaattttgaa ttaaatactg tctcaattta
     3481 aagtcgagtt ctttaagtga aatctcttct ttataaatgt agtgtactct accgtacgta
     3541 gcaataccgt caccttcatc tctcttgatt tgaaatcttg gtgcgtttat ataatcataa
     3601 taagcgtctt gatttttctt agtgacacca ccatatgaaa acactgtgcc attacggttt
     3661 tccgcttctt taacaacaaa tatgtctaat cccggatttt tacgtgcttt aaatctttca
     3721 atatctttac caaatatctg tactcttgtg aattttctat ttttatcaaa gataaggtaa
     3781 tgcttgccac ctttgctata acgataacca gtaacatttt taagttcctt acttgcgcca
     3841 ctatagtaat ctcttaagtc aaagatatct tttgtcacat tttcatattt tgctttatgt
     3901 tcactcgcat ttacagtttg atgcaatgac gttattgttc ctgttgctaa aatacctaat
     3961 gctaaacttg ctttcgcaat tgctgtcatt ttcatagttg tatgctccat tcgtaattat
     4021 tagatttgtt cgcttacgtc tattgaatca tacagcttta ttatagttag cgtatttgac
     4081 ctttcacatt aaaccatgtt taataatcat tgaatcatta ttaagtaaat taaggaatct
     4141 ataatgttcg ttaaataaaa ctgatcccgt tgtgcttcac acccgataga tagggattta
     4201 cagataaatt caggtctctt ccacgtcata tttggaccca tcgaaaattc gggttctcaa
     4261 atcatcgaac ataacaaaag aagctaagca acatgtaggc cgttgtcact taacttcttg
     4321 tttttccgat gacagcttct atttagagaa tgtcatgatt attttatatt cacttcaatg
     4381 ttatcaatat tagtgccatc tatgacatct gccatgcgat tttcttgtaa ttttttgtgc
     4441 aattcaaacg tgtactttcc accgtttttc attttaataa caattttacc tgaaccaacg
     4501 ttaccgtaca gattattttt ttcaataagt tgttttctca atttaaaatc aagttctttc
     4561 aaggaaatct gttctttagt aatcttgaat tctgaaacat catgagagat tgtaccttta
     4621 ttatcttcct tagtaattct tactcctgct ttgtgatcaa cttttttact attactcttt
     4681 gtgataccac cgacagaata tttttccaga ttgtaattat tttcttctaa aacgacaaat
     4741 acatcgacat tcctatgtac tccttcacca tattttttat catctttacc aactaaagca
     4801 attttatata tgaaataatc tgggacaaca ttcataaatc ttattgtcgt ccattttttt
     4861 aaaataatac caatctcatt tttaaattct aaacttggtt tcgtataata cgctcttaaa
     4921 tctttaaatt taggatttat ttctgttggt acttgttttg tggttggcga ttgtggtgtg
     4981 tctgatttag tagattgcat tggttgtggc gtgtttgttg atggaggtgt tgtcacttta
     5041 gttgaaggcg gtgttgtcgc atttgctgtt tgttgcggtg cttctacttt agttgagggc
     5101 ggtgttgtcg cgtttggttt tgattgcggt gcttctattt tagttgaggg cggtgttgat
     5161 tgtggtgctt ccactttagt ggaagatggt gttgtcgcgt ttgctgcttg cgttgtcgtt
     5221 gtgattacac ctgttgttaa aaggcctagt gctaaacttg ttttagcaat cgttgttatt
     5281 ttcatagttg tatgctccat tcgtaattat tagatttgtt cgattacatt cattgaatca
     5341 tacagcttta ttatagatgg cgtattgctc cattcacatt aaaccttgtt taactatatt
     5401 tgaatcatcg ttaagtaaat taagaaatcc ataatgttcg ttaaataaaa atgattttga
     5461 tgtgattcaa cacttggcac atttgaagtt tcgtcacttt taagacatag aaatgccact
     5521 tttacaaaca aatgaatatt cgtcttttta catcattacg cataataaaa gaagctaagc
     5581 aacatgtaaa ccgttgtcac ttaacttctt gtttttccga tgacagcttc tatttagaga
     5641 atgtcatgat tattttatat tcacttcaat gttatcaata ttagtgcctg ccatacgatg
     5701 ctcttgcagt tttttgtgta attcaaacgt atatttccca ccgtttttca ttttaataac
     5761 gattgttcct gaacccatgt taccgtaaag attatgtttt tcaataagtt gttttctcaa
     5821 tttaaaatca agctctttca aggaaatctc ttccttagta atcatgtatt ctgaaacatc
     5881 gcgtgaaatc ataccttgat tatctttttt agtaatgctt aattctactt tgtgattaac
     5941 ttttttacta ttagtcttcg tgatgccacc gacagaatat tttttcaatt gatatttatt
     6001 gtcttctaaa acgataaata catcgatatt atcgtaaggt ccatctttat attttttctc
     6061 atcttttcca actaaagcta ttttatagat gaacctattt ggaataacat tcataaacct
     6121 aaccgtcgtc catggtttga gcataaatcc aaactgcttt tcaaattcaa aactcggttt
     6181 tgtataatac gctcttaaat cttcatattt aggagtcata tctgtttgtg cttgttttat
     6241 ggttggagat tgtggtgtgt ctgatttagt agattgcatt ggttgtggcg tgtttgttga
     6301 tggaggtgtt gtcactttag ttttcggcgt tgtggattcg gttgtcgttt gtgattgttc
     6361 ttgtttaggc gctggcgttg ctgatatatt aagcgttttc tgctcttctt gtttaggttg
     6421 tgatattttt tctattttgg aagctgaggt tttttcctca ttagtatttg gtgccttttc
     6481 gagtttaggc gtgcgttctt gtcttgtgtt agctgcttgt gttgtcgctg aatttgcacc
     6541 tgctgttatg tttatcattg ctaatcgctc tgctttaagc gttggtactt tgtcaacttt
     6601 agttgattgt attttttctg ctttgaccga ttgcgtcgtt actgtaattg cgcctgttgt
     6661 taaaagccct agtgctaaac tggttttagc aattgttctc attttcataa ttgtatgctc
     6721 caatctatat tatattcgat tgtcttttta cgtaatttga atcatacaac atcattatag
     6781 atggcgttct aagataatca cattaaaccc cttttaacaa ttattgaagt attattaagt
     6841 aatttaagca aaaaataatg agtgagtatg agattaatat agcgtttcta tgtgcctttg
     6901 aaataatttt taagcattaa aaagaagtta agcaacgttt gatcgtcact taacctctct
     6961 atttcaattt caacttattt cgtcatcaag tatatgtgtt atgcttttat aactttgatt
     7021 tcaattctat caatatctgt gacattgata acatcggaca tacggtcttc ttgtaacttt
     7081 ttatccaatt caaatgtata ctttccatag tatttctttt tgactgtaat ttttcctgta
     7141 ctcatttcac cgtaaagacc ataattatca ataaggtatt ttcttaattt aaaatcaatc
     7201 tctttcaatg acatcgcttc tttatctatt ttaaatggga aaaagtcata atcatattca
     7261 ccagtatgat cttctttaat aactcttgct tctgctatta ggtcgacagc tttatcgttt
     7321 gcactcgtga tacccccaat agagtacttt gcaccttcaa atctcttatc ctcattaacg
     7381 taaaatatat taagattacg atgtacaccc gtatgataat gttgcttatc tttgccaatt
     7441 aaagcaatat tattaacaga attaccatct atgatattca taaatttaat acttggttga
     7501 atgaaactga tataacctgt cacattttta tattcaatac taggttgatt ataataagct
     7561 tttaattttt tgctattttc acttattaca ataggtttct tttcggcatg aactggtttt
     7621 tccgttgtag tgtttacacc tgttgctaat attcctaata acaaacttat tttt
//
LOCUS       DAKTIP010000123         7614 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAKTIP010000123 DAKTIP010000000
VERSION     DAKTIP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.123"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            103..1464
                     /locus_tag="O0305_001921"
     CDS             103..1464
                     /locus_tag="O0305_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCY0275061.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            1451..3124
                     /gene="menD"
                     /locus_tag="O0305_001922"
     CDS             1451..3124
                     /gene="menD"
                     /locus_tag="O0305_001922"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCY0275062.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            3111..3914
                     /gene="menH"
                     /locus_tag="O0305_001923"
     CDS             3111..3914
                     /gene="menH"
                     /locus_tag="O0305_001923"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCY0275063.1"
                     /translation="MTHYKFYEANVETNQVLVFLHGFLSDSRTYHNHIEKFTDNYHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            3907..4728
                     /gene="menB"
                     /locus_tag="O0305_001924"
     CDS             3907..4728
                     /gene="menB"
                     /locus_tag="O0305_001924"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCY0275064.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(4967..5296)
                     /gene="sspC"
                     /locus_tag="O0305_001925"
     CDS             complement(4967..5296)
                     /gene="sspC"
                     /locus_tag="O0305_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCY0275065.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(5334..6515)
                     /gene="sspB"
                     /locus_tag="O0305_001926"
     CDS             complement(5334..6515)
                     /gene="sspB"
                     /locus_tag="O0305_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCY0275066.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(6597..7607)
                     /gene="sspA"
                     /locus_tag="O0305_001927"
     CDS             complement(6597..7607)
                     /gene="sspA"
                     /locus_tag="O0305_001927"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCY0275067.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNNNSDNPD
                     AA"
BASE COUNT         2508 a         1115 c         1422 g         2569 t
ORIGIN      
        1 gaaaaattgc aagataagtt taagcaattc ttgtaacaca attatccaaa aatgatacaa
       61 tataataatg agtgaaaaaa gacgaagaaa gaagtgaaat ggatggctac gggcgtatta
      121 gaggacgata ttgtcaaaga gatatatggc agctcaaagg aatgggtttc agttgaagtg
      181 aaattatcac agtcactaga cccgagcaca ttatttcatc tcactgacaa tgaggcagga
      241 gatcgctttt atatgcgttt gaatgataat cgaacgtcat attttggcta caaagcaatt
      301 caattattca aaaataattc taaaaataaa caatctattt ttaaagactg ggaaaaatta
      361 aaacataaca tcacatttat acatccgcaa tctgagaaac atcatcttcg agttgttgga
      421 gggttccaat tttcaagtca taaatcagat gatgaatggc gagagtttgg actaaatcat
      481 tttgtattac ctgaagtttt aatttcaact gataataatg ggacattttt aacttataca
      541 gttaaaaggg aaagttttac tgttgaggca ttgaacgatt taatggattt gttcaacaat
      601 atatcggaca tagatgtgga cgagcaaatt gggggaatta ctagaaatga agatatttat
      661 aaagatgact ggcgtcaact tgtagtagaa gctatagaat ctattaataa tgaagaaaaa
      721 attgtactag cacgtagacg gttaataaag ttcgataaag atatcagtat tccatatatt
      781 ctaaagcaag catattctaa agaaaaaaac agttatatat tcttgttaga atcacaagat
      841 tctatattct tttcacaaac acctgaacaa ttaataaagg tcaataataa aatactatcg
      901 actaaagctg tagcaggtac aattaaacgt tcacaagatg aggacgaaga tacaaaaaat
      961 gttgaagcat ttttaaaaga taataaaaac ttaatcgaac atcgatttgt tgttgacagt
     1021 attttacatg atattaaacc ttatatcact gaattacatt atgataagac gcctaaaatt
     1081 ctaaaaaatg atcatttata tcacttgtac actgaaataa aggcgccact gaaggatgat
     1141 tcgtatatta gtttaattga taatttacat ccaacacctg ctttaggtgg ctatccaaaa
     1201 gaatttgcaa tggattttat tgagcagaaa gaatttggta cgcgaggatt atatggtgca
     1261 ccggttggct atatagatat atacgatgat tgtgaattta ttgttgcaat tcgttcgatg
     1321 cttattaaga aagcacaagc aactttattt gctgggtgtg gcattgttaa agattctgat
     1381 ccagatagtg aattggcaga aacgaacctt aagttcacac ctatgatgaa tgcattagga
     1441 gtcgatatga atgggaaatc ataaagcagc tttaacgaag caagttttta catttgcatc
     1501 tgagttatat gcgtacggcg taagggaagt agttatcagt ccgggatcac gctcaacgcc
     1561 acttgcactt gcatttgaag cacatccaaa tattaaaaca tggatacacc ccgatgagcg
     1621 aagtgcagcg ttttttgcag ttgggttaat taaaggcagt gaaagacctg tcgctatatt
     1681 atgtacgtca ggtacagcag cagcgaatta tacgcctgca attgctgaaa gccaaattag
     1741 tagaattcct ttaatcgttt taacaagtga ccgtccgcat gaattaagaa gtgtaggcgc
     1801 accacaagcg attaatcaag taaatatgtt taataattat gtaagttatg agttcgatat
     1861 gcctattgcg gatgatagta aagagaccat tgatgcaatt tactatcaaa tgcaaattgc
     1921 tagtcaatat ttatatggac cacataaagg gccaattcat tttaacttgc catttagaga
     1981 tccgttaaca cctgatttga atgcaacaga attgttaact tctgagatga agattttacc
     2041 gcactatcaa aaaagtatag atgcatcggc attaagacac attttaaata agaaaaaagg
     2101 tttaattatt gtaggggata tgcagcacca agaagttgat caaatactaa cgtattcaac
     2161 gatatatgat ttgcctattt tagctgatcc tttaagtcat ttaagaaaat ttgatcatcc
     2221 gaatgttatc tgtacatatg atttgctgtt tagaagcggc ttagacttaa atgtggattt
     2281 cgtaattcgt gttgggaaac cagtgatttc taaaaagtta aatcaatggt taaagaaaac
     2341 tgatgcattt caaatattag tgcaaaacaa tgataagatt gatgtctttc cgatagcgcc
     2401 agatatttca tatgagattt ctgcgaatga tttctttagg tcattaatgg aagacacgac
     2461 agttaatcgc gtaagttggt tagaaaaatg gcaatgctta gagaaaaaag ggcgtaaaga
     2521 aattaaatgt tatttggaac aagctacaga tgagagtgca ttcgttggtg aattgattaa
     2581 gaaaacatct gaaaaagatg cattatttat tagtaatagt atgcctatca gagatgtaga
     2641 taacttgtta ttgaataaaa atatagatgt ctatgcgaat cgtggtgcga atggtattga
     2701 tggtatcgtt tcaactgcac tgggtatggc tgtgcataaa cgaataacat tattgatagg
     2761 tgatttatca ttttatcatg atatgaatgg actattaatg tcaaaattaa ataatattca
     2821 gatgaatatt gtattattga acaacgatgg tggcggtatt ttttcatatt taccacaaaa
     2881 agaaagtgca actgactatt ttgaacggtt gtttggcaca ccgacgggat tggatttcga
     2941 gtatacagct aagttatatc aattcgattt taaacgtttt aacagtgttt cagaatttaa
     3001 aaatgccaca ttgttatctg aaacttcgac gatttatgaa ttgataacga atcgcgaaga
     3061 taactttaaa cagcatcaaa ttttatacca gaaattgagt gaaatgattc atgacacatt
     3121 ataaatttta tgaagcaaac gttgagacca atcaagtttt agtatttctg catggatttc
     3181 ttagcgacag ccgtacttat cataatcaca tcgaaaaatt tactgataac tatcatgtca
     3241 tcactataga cttaccaggc catggcgaag atcagtcttc aatggatgaa acgtggaatt
     3301 ttgattatat tacgacgttg ttagaccgaa ttttagataa atataaagat aaatcaataa
     3361 cattgtttgg atattcaatg ggtgggcgtg ttgcattata ttatgcaatt aatggtcaca
     3421 tccctatatc taatttgata ttagaaagta cgtcaccagg tattaaagaa gaagcaaatc
     3481 aattggaacg ccgtcttgtt gatgatgcac gtgctaaagt attagacata gcaggtattg
     3541 aattatttgt taatgattgg gaaaagttgc cattatttca atcgcaacta gaattaccag
     3601 ttgaaataca acatcaaata agacaacaac gattgtctca atcgccacat aaaatggcca
     3661 aagcattaag agattatggt acaggtcaaa tgccaaactt atggccgcgc ctgaaagaaa
     3721 ttaaagtacc aacattaata ttagctggag aatatgatga aaaatttgta cagattgcga
     3781 aaaaaatggc aaatttaatt cctaatagta aatgtaaatt aatttctgct acaggtcata
     3841 caattcatgt ggaagatagt gatgaatttg atacaatgat attaggattt ttaaaggagg
     3901 agcaaaatga ctaacagaca atgggaaaca cttagagaat atgatgaaat caaatatgaa
     3961 ttttacgaag ggattgctaa ggtaacaata aatcgccctg aagtacgcaa tgcgtttaca
     4021 cctaaaacag ttgctgaaat gattgacgca ttttcacgtg cacgtgatga tcaaaacgtt
     4081 tcagttatcg tattaactgg tgaaggtgat ttagcattct gttctggtgg tgaccagaag
     4141 aaacgtggac atggtggtta tgtaggtgaa gaccaaatcc ctcgcttaaa tgtattagat
     4201 ttacagcgtt taattcgtat tattccaaaa ccggttatcg cgatggtaaa aggttatgct
     4261 gtaggtggcg gtaatgtact aaatgttgtt tgtgacttaa cgattgctgc tgataatgct
     4321 atttttggac aaactggtcc taaagtaggt tcatttgatg cgggttatgg ttcaggatat
     4381 ttagcacgta tcgttggaca taagaaagca cgtgaaattt ggtacttatg tcgtcaatac
     4441 aatgcacaag aagctttaga tatgggtcta gtaaatacag tggtaccttt agagaaagtt
     4501 gaagatgaaa ctgtgcaatg gtgtaaagag attatgaaac actcaccaac agcgttacga
     4561 ttccttaaag cagctatgaa tgctgacaca gatggtttag ctggtttaca acaaatggct
     4621 ggggatgcaa cattgcttta ttacacaact gatgaagcga aagaaggccg tgatgcgttt
     4681 aaagaaaaac gtgatcctga cttcgatcaa ttccctaaat tcccataagt tattttgaat
     4741 atgatattaa gtcacttgcg tcgtttatta gcgacgatag gtggcttttt tattttataa
     4801 gaatttaaaa gggcatttta attaattatc ataaagataa gatagtttga gaaaaattaa
     4861 tagagaaaga ataaatgtta aagattatta ttacaatgaa aaaacgagct ggtgagaaat
     4921 tgaattctcg ccaacccgct ttatgaagtg aaatcattta aattagttat actaagcgct
     4981 cataaacgat tggtcgcgaa gtgccaatac cttgtttgga tgacgtttgc atgaggatat
     5041 gctgtgtatc atcataatca agaatataaa tgatttcttc attatcaata gatgaaaatt
     5101 taatgcgttg atgtgccgta tctataaaaa gaatatgata ttgattgtga cttgtatcat
     5161 cgccatgacg tatacaaatt gatttttgta attgatgatt actccaatta ccaatgaata
     5221 aattgatatt ggtttgttct agatgtgtga gtttggttgt gtcgtaaact aaatttataa
     5281 attgtagttg atacatagtt ttacctgcct ttaatcaata tctatattac tttttagtaa
     5341 cctatcattg aaccatacca gttataatca cgattgaatg ataaatgtaa taggcttgaa
     5401 tctgcatctt ggattgataa ttctgtatcc caaggattcc agtaaataag tttttcttgg
     5461 tcattaattt tagcattacc aacaactgct agcgcatgtc ctaaatgtgg gtcattaggg
     5521 ttttgagata cactttgtgc aaggatcata attcctacat tatcttttgt aagttgatca
     5581 acttgttcat atgatggtac gccttcttga taatgaatat ctctgccttg tgatttaccg
     5641 tattcaatca tttgattagg gaatgttgca caattaggaa ggtcttgctc acttacttca
     5701 gggtataatg tacgcataat atcatgtgca ttataagtgt ctgtattttt agttgcattt
     5761 aataatgctg ccatactgaa tcctgcacac catgagttat cgaattgttg ttctctaatt
     5821 ttgaagtttt ttaatgtatt ttcatattga acttgatctt cttgtatcgc ttcgttttct
     5881 tcaactttag taggtgttac tgtatttttt aactcttgtt tcaattttgc tgaaactgtt
     5941 ttagcacttt ctttttcttt tacattacca ggtagaggcg tagcttttac taatctaact
     6001 ttgccatctt cttcaaaata aaaccctttt tcatcagtaa gaacagtgat atttgaattt
     6061 ttatctttaa tttggtctaa atctttagcg atgaagtttg aaattttaac actgtaattc
     6121 atgtcttctt ttgatttgtt taaatcatct ttatttttag gactaagtgt taaagtataa
     6181 acaattttac cgtcttttat aactggataa tagtaactat tatcttcttc accattaaat
     6241 ttataaattt taaaagcttc gccaagttca tatttaccag tttttgcatt actttgttta
     6301 tctaatgctt tagcataacc agcaaattgt tgttgtgcta gatcttttac tttttgaggt
     6361 actttgtcac tcttaacatt aatttctagc tgtttagagt gtgaatcggc tttggcttta
     6421 ttgttattag caaatgcgcc tagtgataaa ataagcattg aaaccattat gatgcttata
     6481 atattgaata ctctagattt acatgaacta ttcatataat accctccatt taaatcaaaa
     6541 tattttacgt tgctaagact ttgcttctat tttatctaga cgaacttaat tgttaattaa
     6601 gctgcatctg gattgtctga attattgtta tcgccattgt ctggattatc agggttgtta
     6661 gggttgtcag ggttatttgg ttcatctggg ttgttagggt tatcaggatt gttagggtta
     6721 tcaggattat ctgggttatt aggttggtca tcgttggcaa aatggatatc ttcaatattt
     6781 tgttttaaga agttgcgtac attttcatta ataaataccg caccattaaa ttcatttggt
     6841 acaccgcccc aatgaattcc gatcacttca tttttttcat taaatacagg tgaacctgaa
     6901 ttaccaccag ttgtacttaa atcatattgc atagcttcgc ctttgaggta agtgattttt
     6961 cctttacttt cccacattgt tgctacaggt ttatcaccag gatatcctgt tacagtaata
     7021 ttttggttaa cttgtgtttc agcattatta ctcattgttg ctggtttaac tacttcacca
     7081 atatgtttgt tttgctcatt aggggagaat ttaactattg ctaaatcacc ttcgcctgaa
     7141 tatttagtga tttgttcagc agtgaaacca ccatttggat aattgtcttg gttaattgca
     7201 gaagggaatg cttttaaagc atgaggatca ccgtgcgtag catctacgac gtgtttattt
     7261 gttaaaagag tatctttacc tacaactaca ccggaagcaa taaatgtacc agtaggtgct
     7321 tcaacttgaa tataagttac gggtgcataa tgaccattcg ttgtatctgt gatttggtga
     7381 cgatcgttat ttggtaatat aacatttgcg tgttcacgtt gttctaatgg tttaaggtta
     7441 ccgccttttt gaatcttagg tgtttgctgt ttgcttgact gcgtttgttg tggatgattg
     7501 tccatagcct ttgaagataa cgcgtttgct gctggagaac tcacaagtgt cgctgttgtc
     7561 aaagttgcaa cgaataaaga actaactttt aaaaatttac ctttcatcta aaaa
//
LOCUS       DAKTIP010000124         7603 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAKTIP010000124 DAKTIP010000000
VERSION     DAKTIP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.124"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(178..1905)
                     /locus_tag="O0305_001928"
     CDS             complement(178..1905)
                     /locus_tag="O0305_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275068.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            2326..3621
                     /gene="pbp4"
                     /locus_tag="O0305_001929"
     CDS             2326..3621
                     /gene="pbp4"
                     /locus_tag="O0305_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCY0275069.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQATNSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWEEHPLFTIIGGTCLVAGLALIVHMIINRLFRKRK"
     gene            complement(3738..4136)
                     /gene="tagD"
                     /locus_tag="O0305_001930"
     CDS             complement(3738..4136)
                     /gene="tagD"
                     /locus_tag="O0305_001930"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCY0275070.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(4199..5260)
                     /locus_tag="O0305_001931"
     CDS             complement(4199..5260)
                     /locus_tag="O0305_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCY0275071.1"
                     /translation="MRFTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVIKPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNEE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            complement(5257..6360)
                     /locus_tag="O0305_001932"
     CDS             complement(5257..6360)
                     /locus_tag="O0305_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCY0275072.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDQVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            complement(6459..7271)
                     /gene="tagG"
                     /locus_tag="O0305_001933"
     CDS             complement(6459..7271)
                     /gene="tagG"
                     /locus_tag="O0305_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCY0275073.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
BASE COUNT         2554 a         1329 c         1084 g         2636 t
ORIGIN      
        1 tttcttaaaa tatcgatttc attaatttaa gaattattat gtcgtaactt ctcaaatgat
       61 ttaaaataga caaaagcttg aagcaattta ttaacatcta tatccatcaa tgtgccacac
      121 tttaatttcc tcgatggtat atgtgtattt gctccaagct tacttatata tataaaatta
      181 atctgttaat ttttgagaca ctacaaagtt tttatacttc gcatgtgatg ccatcagttc
      241 tgaatgcgta cctttacctg ttacctgtcc tttgtctaag aatataattt gaccggcttt
      301 tttaattgta gacaaacgat gcgcaatgac aatcgttgtt ctaccttcca tcaatgtttc
      361 taaagcttct tgaattttca attcactttc actatcgaga ttagctgttg cttcatcaag
      421 taacaaaata tcaggatttt taacaaaact tctagcaata tcaatacgtt gacgttgtcc
      481 gccagacagt ttcaatcctc gttcacctac aagcgtgtca tatccttcat caaattgcat
      541 gataaaatca tgacagttcg ctaatttagc ataattaata agttcttcat ctgaaacatg
      601 acgattaatt ccgtataaaa tattgtctct aattgtacca ctcatcatcg aatttgattg
      661 cataacatat ccaattttgc gtcgccactt agataacggg atatcataga cactttcaag
      721 gccatattta atatcacctg actcaatttc atacatacgt tctatcagat taaatatcgt
      781 acttttacca gacccagaag ggcctacaaa agcactcact tgaccttgtg ggatttggaa
      841 cgacacatca tctaatattt tcttcacatc atatttaaag tctacatgtt caaatgacaa
      901 tacaccgtca tcaattaata cattttcaga atcttcaaga gcttctgtcg gttcaatagg
      961 ttcttgcatg atttcgtata ttctactact tgcaccgact gcctttttat aatctgtaac
     1021 taacgtggaa agattgatta aaggcataga taactgaata acataaaata tcattgcaat
     1081 taatgtacct gcagtgattg caccagtcgc aatttctaat gcaccaaaac ctaaaataat
     1141 tgcaattgtt agcaacataa ctatacctga aattggttgt acaactgccg caattttagc
     1201 ctgttttaaa cctaatttat atatttcatt caaattttta tgtgcattat ctaattcaag
     1261 acgctctgta tttgatattt taacaagacg catttcagtt aggacacgcc ctaacaaacc
     1321 actgaagttt gcaatttcag attgtgtact tgtcgatatc ttttgcataa tacgacctag
     1381 aggaatcata attaaaacga atatcggtat cgttataaat gttaataatg tcattttcca
     1441 atctaaaata aataacatga ttagtgaccc aactaatgta acgattgatg gtaataagtt
     1501 aggtagcttt tgtgaaataa attcatttat cactttcgta tcgtcagtta atcgactcat
     1561 taattgacca ctttcatttt tgtcaaagaa tggcattttt aattgtatga tatgctccca
     1621 taaaactgag cgtatcgcat aaataatctt ttcaccaatt ttacttaata aatataaacc
     1681 taatccgctt aataaagcat tgatgacaaa gataccacca aataatgcga ttagattcca
     1741 attgatatgg ctcacggaaa atttatctac aattcgtcca gtaaacagtg gcactaatag
     1801 tccacttaag ctccctagtg atgaaatagt gatagctgca acgataagac ccactggcca
     1861 tgatagtttt ttaaataaga aaaacaatgg attttctcgt ttcatagttt tacctcttct
     1921 gtttgaaatt tatagttaat tcatttagat agatacatgc atttctatca tatactttct
     1981 cccaacaaaa ctttaaaaat caaataaagt gttgccccaa aataccgctc ataacattta
     2041 tatgatagaa tatttctatt gcattttttg taataacttc tcaaagtctt tatattaaaa
     2101 catgaaaatt aatcgctttt gagtaagttt gctcttcgta caaacattat aacaccttta
     2161 gctacacacg taatatattt gcattatttt tcaaattaac tgcagaaaac tttattttca
     2221 acaatttaat taaggtgaag tgaattagca atgatttccg taagtaggaa taaccattgc
     2281 ttttagggaa tatttaagaa ttggaaaagg gaagattaac gctttatgaa aaatttaata
     2341 tctattatca tcattttatg tttaacatta agtattatga caccatatgc acaagctact
     2401 aacagtgacg taacccctgt acaagcagca aatcaatatg gttatgcagg tttgtcggct
     2461 gcatacgaac cgacgagtgc tgttaatgta agtcaaactg gacaattact gtatcaatac
     2521 aatatcgata ctaagtggaa tccagcgtct atgactaaat taatgacaat gtacttaaca
     2581 ttggaagctg taaataaggg gcagctttca cttgatgaca cagtcacaat gacgaacaaa
     2641 gaatatatta tgtctacact acctgagttg agtaatacga aactatatcc tggacaagta
     2701 tggacaatcg cagacctatt acaaattaca gtatctaatt ctagtaatgc cgcggcatta
     2761 attttagcta agaaggtttc aaaaaacacc agcgatttcg ttgatttaat gaataacaaa
     2821 gctaaagcta tcggaatgaa aaatacacat ttcgtcaatc caacgggtgc tgaaaattca
     2881 agattacgta catttgcacc aacaaagtat aaagaccaag aacgtactgt aacgactgct
     2941 agagactatg ccattttaga tttacacgtg attaaagaga cacctaaaat attagacttt
     3001 acaaagcagt tagcaccaac aacgcatgca gttacgtatt acacattcaa cttttcattg
     3061 gaaggtgcaa aaatgagttt gccgggtaca gatggtttaa aaactggatc aagtgataca
     3121 gcaaattaca accatacgat tactaccaaa cgaggtaaat ttagaattaa tcaagttatc
     3181 atgggtgcag gagactataa aaaccttggt ggcgagaagc aacgtaatat gatggggaat
     3241 gcattaatgg aacgctcatt tgatcagtat aaatatgtaa aaatattgtc taaaggtgag
     3301 caaaggataa atggtaagaa atattatgtt gaaaatgatc tttacgatgt tttaccaagt
     3361 gattttagta aaaaagatta taaacttgta gtcgaagatg gtaaagtaca cgcggactat
     3421 ccaagagaat ttattaataa agattatgga cctccaactg tagaagttca tcagccaatt
     3481 attcaaaagg caaatactgt tgctaaaagt atgtgggaag aacatccatt attcactatc
     3541 attggtggta catgccttgt cgctggatta gcacttattg ttcatatgat aatcaatcgt
     3601 ttatttagaa aaagaaaata aaacatacta aaaacggaca agttgcacat tataaagctg
     3661 cgaaacttgt ccgttttata tttattttat aataacctaa gttaatttat cgtttagtat
     3721 cgatagttct atataattta tttagcatct ttaccatata attcttgttt gattttagtc
     3781 gtcgaaatgc cttctgtacg ttttaaataa atgacttcac atttatcctt taagaagtcg
     3841 aattcacctt cccagtcatg tcccataaca aaaacatcta catcaaattt ttcgacatcg
     3901 tcttcttttt gtccccagcc cttttctgga atgactaaat cgacatagcg tattgattca
     3961 agcatcattt ttcgttgttc ataatcataa taagattttt tatgtttaat ttgattaaat
     4021 tcatctgttg ataatgctac tattaaataa tcgcccatct ctcttgcacg acgaagcaat
     4081 tcgatatgac catagtgaag taagtcatat gtgccatatg ttattacacg tttcattaag
     4141 ttatcctcct catattgtca catcatagga ttgcagttta tggtcatcaa tgtacacgtt
     4201 atcgttttaa cattgatttt gttcgatgtg caaactttct ccctttaagt tgtatcaaga
     4261 attttttata agtcggccta ttaatgccca cttgaattaa tgtttgtcgc cacaattcgt
     4321 atatttctct atcccaattc tcaccaataa attcaactgt atttataatg tcaaacatgt
     4381 tttgcgatac ataatcagaa tgctgcatag acataatata gtcttttacg agttgaattg
     4441 tcgtgtgcgt tactgctggg tgttcgttca tataatattt taatgaatta ttgaataaaa
     4501 tacgatcaac catatcacta tataatagcc tttgttcagg taatagtaac atttccatga
     4561 ctcgttgtct tattttcata atttctgtga tatatctatt aaaatcttct tcgttacttg
     4621 tagcatgtgt atctatatcg ttatctccga caatcatctg tgaaactaaa tgtatatccg
     4681 tagctttttg catcgccttg acaagtattg cgtgattgta tgtgttagct aaagtttcgt
     4741 cacactgtaa ttcagcaaat ttagcactga ataactttcc gtcaaaagac atcattgata
     4801 acaaatctgg ttgttccgtt aatgtaacga ttcgattgtc tttaataaac tcatcagcac
     4861 ttttcaattt ccattcacca tggtaattgt aagctcgctg ccctattacc atatcaatat
     4921 tatcattatt tttgaaagca gcagttaacc tatctaattt acctggcaat acgattgatg
     4981 ttggttttat gaccataaat gcgtcactat catgtggatc tgtcattttg atatccatca
     5041 aacctttatt aatacacgta gctatcgaag cattttgtaa ttgtattaat gaaatatgct
     5101 tgagttctct ttgtaaccgt tcaatcatag gaattgtttg atcagtagaa ccaccatcaa
     5161 tacaaaggat tctatagtgt tctttactct caatagatat taacaattgt cgaattgttg
     5221 cctcattatt acatgtaggt atgattatcg taaacctcat tttgtcacca tcttatctat
     5281 atattctgtg agctgatgta aacttttatc agtattatac ttatgccaat ctttaaataa
     5341 cggacttaat agatgttctt tttcttgtat cgtcattatt aaatcttctt cagtatacac
     5401 tttgtagcta tccggtattg ctttgtaaaa ttgattcagg cctctcacct gatcatatgt
     5461 tccttcatca tacacataaa atatagttgg aatatctaac aagctagctt ctattggcag
     5521 cgaactatag tcgctaataa ttatatctga cattagcatt aatgtagacg tgtcgattga
     5581 agatacgtca tcaatgtctg aatcttcaat tgatggatgt aatttattaa tcagtgtata
     5641 tcctggtaaa catttttcaa aataagcttt atcaatagcc ctattatctg ctttatcttc
     5701 tctatatgtt ggtacatata ataccaactt atttgtaatt ccatatttat cctttaactc
     5761 tgccttaacc gttgctctat cagctgtgta atatttatta attctcggaa gcccaaaata
     5821 cagcatttgc tcttctgttg cacctaaaga ctgtttaaaa cattgtgaca tttgttcaca
     5881 acccactaag taaaaatccg tcgcttgata aactttacgg tactgctgaa ccattgcctt
     5941 gtcagacaca tcgacttgat gatctgttaa gccaaagttt tttaatgcac cacttgcatg
     6001 ccacgtttga acaatgtgtt gattagaagt cttattatat ccacctagta ataggtaata
     6061 attatcgata ataatcatct gcgcgctttt caaagcctta atttgtttta ccaatgttcg
     6121 attagtcatt tctatcacat caacatcgtc gctaagttca gataaataag gcgcttgttt
     6181 tggtgttgtt aaaacagttt tctgatacga cgaattattt aatgctttga tgataggctt
     6241 aatatcttct ggaaaagtca tcataaatac gatatgcggt ttatcaatca cttgaggcgt
     6301 aatcatttta gaaagtattc gaactaccaa atgataaaat ttctttatta aaacgttcat
     6361 aatacaccaa cttaatatgt tatttactta aattataaac aaaaatgaac cccacttcca
     6421 tttattaatg gttagcgggg tttcgtcata taaatatatt acaagaagtc tgcaaattga
     6481 tctctatatt tcatgtgtaa gtacgcacca attgcaaaga aaatggcaac aataccgaaa
     6541 ttgtataaca ttaatttcca atgatccatg aaataccatt cgtgatataa aattgctgca
     6601 cggtatgatt cagcaataaa gtaaactgga ttatatttca tcatttcatg aattaaacca
     6661 ctgataccat ggttctttgg tagccacaaa attggtgaaa agtaaaataa tattcttaat
     6721 attgcttgca ttaacatttg tgtatctcta actaacacac cgagtgttga tgttaataac
     6781 gtcaccgagg cagttaagaa aaaacaaaac ggtacatata tcaataattg aatgatatgt
     6841 attgatggat aaataccagt aaacatacat gcaattatca caagtaaaag taagcctaaa
     6901 tgtccataaa atctacttgt cacaatatat gtcggtatta tcgataacgg gaagttcatt
     6961 ttcgatactt gattaaactt ttgtgtaatt gctttagtac cttctaaaat accttggttg
     7021 atgaagaacc acatactgat accaaccaat aaccaataaa caaaaggtac accatgaatt
     7081 ggtgcattac ttcttattcc taatccaaaa accatccagt aaaccataat ttgcataaca
     7141 gggttaatta attcccaagc cacacctaaa tagttactat gattgataat tttaacttga
     7201 aactgagcca gtctttgaat taaataaaag ttctttacat gttctttaaa aactgttcct
     7261 attgctgaca ttccattaaa ccacactttc aaatgtttaa ctatttctct acttaactaa
     7321 atagtattat aataattgtt gtaaatacta tcactataca tggatgctat caaaattatt
     7381 gtctagttct ttaaaatatt agtttattac aaatacatta tagtatacaa tcatgtaagt
     7441 tgaaataagt ttagttttta aatatcattg ttatcattga tgattaacat tttgtgtcaa
     7501 aacacccact ctgataataa caaaatcttc tatacacttt acaacaggtt ttaaaattta
     7561 acaactgttg agtagtatat tataatctag ataaatgtga ata
//
LOCUS       DAKTIP010000125         7550 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAKTIP010000125 DAKTIP010000000
VERSION     DAKTIP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.125"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            49..462
                     /locus_tag="O0305_001934"
     CDS             49..462
                     /locus_tag="O0305_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275074.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDCDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(893..1879)
                     /locus_tag="O0305_001935"
     CDS             complement(893..1879)
                     /locus_tag="O0305_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0275075.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            2407..3165
                     /gene="map"
                     /locus_tag="O0305_001936"
     CDS             2407..3165
                     /gene="map"
                     /locus_tag="O0305_001936"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCY0275076.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            3382..3768
                     /locus_tag="O0305_001937"
     CDS             3382..3768
                     /locus_tag="O0305_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275077.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3783..4484
                     /locus_tag="O0305_001938"
     CDS             3783..4484
                     /locus_tag="O0305_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCY0275078.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            4481..5524
                     /locus_tag="O0305_001939"
     CDS             4481..5524
                     /locus_tag="O0305_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0275079.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            5514..6143
                     /gene="vraR"
                     /locus_tag="O0305_001940"
     CDS             5514..6143
                     /gene="vraR"
                     /locus_tag="O0305_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCY0275080.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMDDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(6259..7476)
                     /locus_tag="O0305_001941"
     CDS             complement(6259..7476)
                     /locus_tag="O0305_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCY0275081.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
BASE COUNT         2692 a         1108 c         1319 g         2431 t
ORIGIN      
        1 atggtgatta tgcaacatga aaaaatgaat gatgaaagga atttgacgat gaagcctact
       61 aaagtgatat taaaagatgc atcttattta catagcaaaa catcgataac atttatttta
      121 aaagatgtag ttatcgaaga agataataaa atttattatt tcgacactag tgcaactttc
      181 gaagatcaag aagttaaatt tgaatttgca ctctttgata gtgacatgga caatttgaaa
      241 catatggatt gcgacaatcc tgttactgaa atatatttta tagaacctga tttacatttt
      301 acaattattg attttaatca agaactgctt tgtatttata ttgattttga ttctggttta
      361 aggcattcaa acatggcaac agaatctggt atttcattaa ggataaatgt tgctaaatca
      421 gattttacta aatttattaa tgaattagcc tctttacatt aatgatttaa atctgatatg
      481 taattacaat caaaaaagac agccacatcc ctccgtagtt taggcgtgtg gctatatttg
      541 agtctgaata tttatgcttg taattttaaa aagggacatg ctatatacga taaaaagagg
      601 cggggacata aatcaatgtt ctatgctcta cgaagttata ttggcagtag ttgactgaac
      661 gaaaatgcgc ttgtaacaag cttttttcaa ttctagtcag gggccccaac aaagagaaat
      721 tggattccca atttctacag acaatgcaag ttggggtgtg ggccccaaca cagagaattt
      781 cgaaaagaaa ttctacaggc aaagcgagtt ggggtgggac gacgaaataa attttatgaa
      841 aatatcattt ctgtcccact cccatggtgc caattagcat aaggtactta aattaagcat
      901 atctgctgtc tagcagtcga taaatcatta gaacttcgta tagtatatga cttttaattt
      961 gattttcacc actaatttca agtgctttta tagtcgaacg taaagtttct acagaatcat
     1021 cttctctctt aaaagaacca tcataaaata tatctttgat gctactacta atttttagca
     1081 atgccatttt ttcgtcacct gaaaagttaa cacgagtatt tttaggcaag taaatgatat
     1141 ttgataaatg agtgataaac aaacgattcg tatatgcacg tttagttaat tgattgagta
     1201 atttccaatc acattctttt ttcttatgat agcttaattc atcacgttga taacttatta
     1261 acgtttcaac ttgattattt aaattgaaaa tatttttata tgctttttcg cttttatcag
     1321 attgcagtct tgataagata agttcttggc agcgattgta aaataattta tacatcaagg
     1381 catctgtctt acttaatttt tcttcgacct gaccataata cttaggtgga aacaccatga
     1441 agttaattaa acctgatgtc acgagtccaa taattgctgt caatgttcga gacaaaaagt
     1501 tgaatatgta ggcatcatga atacctggaa tcatagctaa tgatgttagt acagcgacat
     1561 tcgtaccaac ttgcaatttg agttttgtac agaataaaat cgtgaacgtt gcactcaatg
     1621 catatgtaaa aggtgattga tcgccgaata aatatgtaaa taatactgca aagcctgcac
     1681 caattaccgt agcaggtaat ctacgataac ctttaataag tgatgccttg gcagttggtt
     1741 caattgtgac tacagctgtt aaaatggcat agatgggtgt taaatctagt gccatacaaa
     1801 agacagctgt taaaaaaatg gcaataccag ttttaattgt tctggcacca attaaatgtt
     1861 tataccattg atcgttcatt ttttaacctc taatcatcgt aaaatcttag cgagcgcttt
     1921 ataataatag tatcgtacat tggaaaagtt catgtatgta aaatatttga aataatcata
     1981 cataagcatt actttgattt tcatatacat taatcaaagt tctgggctcc acaatgttat
     2041 attgttagta gttgactgaa ataaaatgca cttgtaacaa gcatttttca attatagtcc
     2101 ggggccccaa catagagaat ttcaaaaaag aaattctaca ggcaatgcag gttggcgggg
     2161 ccccaacaca gaagctgacg aaaagtcagc ttacgataat gtgcaggttg gcggggcccc
     2221 aacatagaga aattggatct acaatttcta caggcaatgc aagttggggt acaacgataa
     2281 agaaatattt tttctttatc acactatgtc tcactcactt tccaaaatac taaagtaaca
     2341 tctttagtat atcaaagaat ttttgctata ataagttata attatataaa aaaggaacgg
     2401 gataaaatga ttgtaaaaac agaagaagaa ttacaagcgt taaaagaaat tggatacata
     2461 tgcgctaaag tgcgcaatac aatgcaagct gcaaccaaac caggtatcac tacgaaagag
     2521 cttgataata ttgcgaaaga gttatttgaa gaatacggtg ctatttctgc gccaattcat
     2581 gatgaaaatt ttcctggtca aacgtgtatt agtgtcaatg aagaggtggc acatgggatt
     2641 ccaagtaagc gtgtcattcg tgaaggagat ttagtaaata ttgatgtatc ggctttgaag
     2701 aatggctatt atgcagatac aggcatttca tttgtcgttg gagaatcaga tgatccaatg
     2761 aaacaaaaag tatgtgacgt agcaacgatg gcatttgaga atgcaattgc aaaagtaaaa
     2821 ccgggtacta agttaagtaa cattggtaaa gcggtgcata atacagctag acaaaatgat
     2881 ttgaaagtca ttaaaaactt aacaggtcat ggtgttggtt tatcattaca tgaagcacca
     2941 gcacatgtac ttaattactt tgatccaaaa gacaaaacat tattaactga aggtatggta
     3001 ttagctattg aaccgtttat ctcatcaaat gcatcatttg ttacagaagg taaaaatgaa
     3061 tgggcttttg aaacgagcga taaaagtttt gttgctcaaa ttgagcatac ggttatcgtg
     3121 actaaggatg gtccgatttt aacgacaaag attgaagaag aatagttcaa catatactaa
     3181 gactaaagta tgaacatcat ttagttccgg agcctattca tattggtttc ggaactgttt
     3241 tataataatt aagaacacaa tcaatgcgtc atttcaaaaa tatgttgtaa caaagtagtt
     3301 tttaagcaaa catatcatcg acatcaacga agatacatag cgcatttggt attttaaaac
     3361 ttattataaa aggtgatagt tatgaactat gttgaacgtt atattgaaca gtttttgaga
     3421 gcaacagtaa gaaataatat caagcactac cttttaatgc tagatgaaaa aatgaaaaat
     3481 ttagatgatt atatgcgtta tttaattact aaaaaagaac aacttagcaa gttaattgac
     3541 agtctaatgc taacattaga aaataaatat attgatattg ctgaagcatt tcaaattcaa
     3601 tgtgcaagag aaatcaataa tcaagaaatt gaaaatatta aatcagagtt gaataaagtt
     3661 gaagcatatt atgcacaaat tgaaactcaa attcaacaaa cttcaactga aaaaatagca
     3721 acagaaaaaa catcgtatct aataaattat atgaacgctg tggcatagaa aggcggcgaa
     3781 acatgacaca caaatatata tcaacgcaaa tgttgatcat ttttactgca ttaatgatta
     3841 ttgccaattt ttactacata ttttttgaaa aaattggctt tttactcgtt ctattattgg
     3901 gatgtgtatt agtttatgta ggatatcttt attttcataa aatacgtggc cttttggcgt
     3961 tttggatagg cgcgctatta attgcattca cattattgtc taataagtat acaatcatca
     4021 tcttgttcgt ctttttatta ttacttattg tgcgttattt aatacacaag tttaaaccaa
     4081 aaaaagtagt tgcgacggat gaggttatga cttcaccatc ttttattaaa caaaagtggt
     4141 ttggtgagca acgtacacca gtttatgtat ataagtggga agatgtacaa attcaacatg
     4201 gaattggcga cctacatatt gacttaacaa aagctgcaaa tattaaggaa aataatacca
     4261 ttgttgttag acacatttta ggtaaagtgc aggttatatt gccggttaat tacaatatta
     4321 atttacatgt agctgctttt tatggaagta cttacgtgaa tgaaaaatca tataaagttg
     4381 aaaataacaa tattcatatt gaagaaatga tgaaaccgga taactataca gttaatatct
     4441 acgtatcaac gtttatcgga gacgtagagg tgatttatcg atgaaccact acattagaac
     4501 aattggttca atgctcatct tagtatatag catgctagct gcatttctgt tcatcgataa
     4561 agtttttgta aatatcatct attttcaagg tatgttttat acacaaatat tcggaatacc
     4621 agttttttta tttttaaatc tcatcatcat attattgtgt attattgttg gttcggtact
     4681 cgcttacaaa atcaatcagc aaaatgattg gattaagacg caaattgagc gttcaatgga
     4741 aggcgaaaca gttggcatta atgatcaaaa tatagaaata tatagtgaaa cgttagattt
     4801 ataccataca ctcgtacctt taaatcaaga attgcataag ttgcgactta aaactcaaaa
     4861 cttaaccaat gaaaattata atattaatga tgtgaaagtt aaaaagatta ttgaagatga
     4921 acgtcaaaga ctagcacgag aacttcacga ttctgttagt cagcaacttt ttgcggcaag
     4981 tatgatgcta tctgctatca aagaaacgaa gttagaacca ccattagacc aacaaattcc
     5041 tattttagag aaaatggttc aagattcgca gttagaaatg cgtgctttgc tgttacattt
     5101 aagaccgctt ggtttaaaag acaaatcttt aggtgagggt attaaagatt tagttattga
     5161 tttacaaaaa aaagtgccaa tgaaagttgt gcatgaaata caagatttta aagtgcctaa
     5221 aggtattgaa gatcatttgt tcagaattac acaggaagca atttcgaata cattgcgtca
     5281 ttcaaacggt acaaaagtga cagtagaatt gtttaataaa gacgattatt tattgttgag
     5341 aattcaagat aatggtaaag gttttaatgt tgatgaaaaa ttagaacaaa gttatggact
     5401 taaaaatatg cgtgaaagag ctttggaaat tggtgcaacg ttccatattg tatcattgcc
     5461 agattcaggt acacgtatcg aggtgaaagc acctttaaat aaggaggatt cgtatgacga
     5521 ttaaagtatt gtttgtggat gatcatgaaa tggtacgtat aggaatttca agttatctat
     5581 caacgcaaag tgatattgaa gtagttggtg aaggcgcttc tggtaaagaa gcaattgcca
     5641 aagcccatga gttgaagcca gatttaattt taatggattt acttatggat gacatggatg
     5701 gtgtagaagc gacgactcag attaaaaaag atttaccgca aattaaagta ttaatgttaa
     5761 ctagttttat tgaagataaa gaggtatatc gtgcattaga tgcaggtgtc gatagttaca
     5821 ttttaaaaac aacaagtgca aaagatatcg ccgatgcagt tcgtaaaact tctagaggag
     5881 aatctgtttt tgaaccggaa gttttagtga aaatgcgtaa ccgtatgaaa aagcgcgcag
     5941 agttatatga aatgcttaca gaacgagaaa tggaaatatt attattgatt gcgaaaggtt
     6001 actcaaatca agaaattgct agtgcatcgc atattactat taaaacggtt aagacacatg
     6061 tgagtaacat tttaagtaag ttagaagtgc aagatagaac acaagctgtt atctatgcat
     6121 tccaacataa tttaattcaa tagttcatat cgaattaaga aaagttactt acgccaatca
     6181 caatataaca tcaaatagac accaaaaaac agaggttttc gcaataatca ttagcgaaaa
     6241 cctctgtttt gttggtgttt agtctttttt aagcttatca acgattttgt cttttatatt
     6301 tttatcatct tctggatagg tatcactagg atgttcatca acatgatacg tacgttgatg
     6361 tgttgcatta ctatcttcat catattcgta tctagtgttc tcattataat gatttttatt
     6421 attgtcatca tgttctaatg ctgcttcttc aggtgtttta cctttaatta cactacgctg
     6481 atgaatgatt gcattgattt cagcaccgac aattataata aaacttgtga tatataacca
     6541 tagtaacaaa atgatgatac ccgcgatact gccatatgtt ttagaatagt taccaaaatt
     6601 tgaaatatac caaccaaaac caaatgaacc agctaaccaa ataattgaag taaatactgc
     6661 acctggtaat actgacttaa gcttcgtttt aacgttaggt gcaaccgaat ataacacgat
     6721 aaatatgata aatataataa tgattggtaa cacaattcta ataaggttaa aaatccattt
     6781 cacttgttcg tcaaatccaa gtggaccgaa taggaaatga ctaattacag aaccaagcgt
     6841 tggtaatgct agagcaacta caaacacaac gcccataact acagtgaaga caacacttag
     6901 tagttttaat acgattccat tacggctatc ttctacatcg taagcaacat tgaaagaatt
     6961 cataattgca gtcattccat ttgaagctga ccaaattgct aaaatcaaac cgatagataa
     7021 taagccacca ctggagtttt gagttatatc accaattaca ctcttaatta gagtagatgt
     7081 ttcagcgggt gcattgctta acatattagt aatttgactc tgcttaatat tgaaaaatgg
     7141 taataatgtt aataggaaaa gcaacatagg gaacatagca agtacgaaat ggtaagtcat
     7201 ttgtgctgct agtcctgagg catcatcttt tcctatgcga tagatgagat atgataagaa
     7261 attagagtct ttcttatatt ttgcaggttt atttaatctt gataagaaga aaacctgatc
     7321 atctttttta ggtttcttag attggaattg ttgaggttca atatatgtac gatcaacatc
     7381 aattttggga ttacttttat ttttcttttt tgactcctct tgcgcttcct taacagaatt
     7441 aaggtatttt gaagaagagt gatccttttt cgacatgtgc actccttaac ctttctctat
     7501 gtaatgtttt ataaagtaac tattagtatt tatccaatta taaaaaaact
//
LOCUS       DAKTIP010000126         7530 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAKTIP010000126 DAKTIP010000000
VERSION     DAKTIP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.126"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1501
                     /locus_tag="O0305_001942"
     CDS             <1..1501
                     /locus_tag="O0305_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0275082.1"
                     /translation="RVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPF
                     QNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLS
                     MFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAY
                     HDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPIT
                     MTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDID
                     FKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQK
                     YSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIY
                     NVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLT
                     NMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      1720..1855
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1982..3025
                     /gene="ribD"
                     /locus_tag="O0305_001943"
     CDS             1982..3025
                     /gene="ribD"
                     /locus_tag="O0305_001943"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCY0275083.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            3032..3664
                     /locus_tag="O0305_001944"
     CDS             3032..3664
                     /locus_tag="O0305_001944"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCY0275084.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            3675..4856
                     /gene="ribB"
                     /locus_tag="O0305_001945"
     CDS             3675..4856
                     /gene="ribB"
                     /locus_tag="O0305_001945"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCY0275085.1"
                     /translation="MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            4869..5333
                     /locus_tag="O0305_001946"
     CDS             4869..5333
                     /locus_tag="O0305_001946"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCY0275086.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(5455..6456)
                     /locus_tag="O0305_001947"
     CDS             complement(5455..6456)
                     /locus_tag="O0305_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCY0275087.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            6568..6687
                     /locus_tag="O0305_001948"
     CDS             6568..6687
                     /locus_tag="O0305_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275088.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            6690..7517
                     /locus_tag="O0305_001949"
     CDS             6690..7517
                     /locus_tag="O0305_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0275089.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2695 a         1063 c         1403 g         2369 t
ORIGIN      
        1 gcgtgtagca ataataggca tgggaactgc tggtgtaagt gtattgagag agttagttaa
       61 gcacccgaag tttaatcaat tagatataga tttatacgat gataaagtaa atatgggaca
      121 gggcgtacca tttcaaaatg atagttctga gctgctaatt aatatgcctt cgaaaaagat
      181 gagtttaaat ttagatgatg agactgaatt ttggaagtgg tataaacaac aaactgattt
      241 taattttgat gaacctgcat atttaccgag atttgtattc ggacattata tgaaatctta
      301 tttatctatg ttcacgaaaa aatatccaaa tatatcaact aattataata aagttcaaga
      361 gatttataca aattctaata tcgatgaaac gaatttaact tattacatat gtactacaaa
      421 ttcaggacaa tcatggcaag cgtacgatta tgtattttta acatgtggta catttgccta
      481 ccatgatcca tataacttaa agggtaaaaa aggatatatc gctacgcctt atccaacata
      541 taatacgtta gacgaagtaa atgaactgga cgatattgcg attattggta caggccttgc
      601 cagtttagat gttgtccgtt atgttgctgc tcatcatcca aaattaccaa ttactatgac
      661 gagtcgttct gctcatttac caagtgtaag aggtacaatg atagacgtga cattcaagta
      721 cttaactaaa gacaaattaa atgacattaa aaaacatcat ttcggcaatg caccgcttga
      781 tactttagtt tctttatttt taaaagaatg tgctgattat gacattgatt ttaaaaaatt
      841 agtacataga cgtacaggaa atcatattgc agatttgaag tatgatttag cgcgtccaac
      901 agaaatgggc attttccaaa gtatgattga acatttgaaa gagaatttaa attggatatg
      961 gaatagctta agtattgaag accaacatca atttaatcaa aaatactcaa agatgattca
     1021 attaaattca aatccaatgc ctccaagaac tgctgaacta attattgaat taatagaaaa
     1081 taagtcttta attttgaaaa aagatttaga agatgtgaaa catgatggca agttgtatta
     1141 tttttcatac aaaaaccaag aatcagtaga catatataat gttgtcatta acgcgacagg
     1201 tgcaaaaagt catttaaatg aattggatca agatgatcaa ttaattagaa atcttgaaaa
     1261 tagacaaatt gtacaagcac acccaatggg aggcatacaa atcattccgg aaaccaatca
     1321 agttattagt ccaaggtttg gaacattaac aaatatgatt gcgataggtc aaatgacaaa
     1381 cggtgttaat aaactacgaa acggtgtgaa aatgattgta gaacaagttg cgcatacagt
     1441 atctcaatta tatgatgctt tagaatcgaa tgagcaacaa cagcgcagtg acaatcaata
     1501 atttgtaact agaagataat aaagagaacg ctctatagag acgaattgaa ggtttgattt
     1561 taatgtctgt tagtaagaat catatcaatg agatgcctat agtactcaga ttatattaaa
     1621 ttaaaaccgt cattaattgt tttttcagaa aacatatagt atcattttaa atgtagttga
     1681 catactacgt actcaaataa tctataacaa tttcatatat aattctttcg gggcagggtg
     1741 aaattcccaa ccggcagtaa ataaagcctg cgacctgcta atatgtttca tattagtggc
     1801 tgatctagtg agattctaga gccgacagtt aaagtctgga tgggagaaag aatgttaatt
     1861 atcgacaaag ataatgtagc gtatttgtaa aaatgtgtac aaataggctt atttaacgat
     1921 aaatttttct ccttgcatct taattcatga tgtgaggatt tttgtttata gaggtgatca
     1981 tttgagtcaa tttatggatt atgcgattca acttgcaaat atggtacaag gtcaaacagg
     2041 tgttaatcca cccgttggcg ctgttgtagt taaagaaggt aggattgttg gtattggtgc
     2101 acacttgaga aaaggtgaca agcatgcgga ggttcaagca cttgatatgg cacaacaaaa
     2161 tgctgaaggt gcgacgattt atattacgtt agagccatgt agtcattttg gttcaacacc
     2221 accctgtgtt aacaaaataa ttgattgtaa gatagcaaaa gtagtatacg caacaaaaga
     2281 caattcgtta gacacacatg gtgatgagac gttacgggct cacggtattg aggttgaatg
     2341 cgttgatgat gaacgggcat cacaattata ccaagacttt tttaaagcaa aagcaaagca
     2401 actgccacaa attacagtga aagtatctgc aagtttagat ggtaaacaag cgaatgataa
     2461 tggacaaagt caatggatta ctaacaaaga ggttaaacaa gatgtctata agttaagaca
     2521 tcgacacgac gcagtgttaa ctggaagacg tacagttgaa ttagatgatc cacaatatac
     2581 tacacgtatt caagatggaa aaaaccctat aaaagtaata ttgtctaagt ctgggaatat
     2641 tcattttaat cagcaaattt atcaagatga atcaacacca atttggatat atactgaaaa
     2701 tccaaattta acaaccaatc aaacacatat tgaaattatt tacttgaagt cttgcgattt
     2761 aacaacaatt cttcacaatt tatataaaag aggagttgga actttgctag ttgaggcagg
     2821 tccaaccact acttcagaat ttctccaatc taattatata gatgaattta ttctctatta
     2881 tgccccgaaa ttaattggcg gatctggaaa ttatcaattt tatcaaacaa atgatgtgat
     2941 tgagatacca gatgcgaacc aatttgaaat tgttcattcc gagttattaa atcaaaatgt
     3001 taaattaact ttacgaaaga agtgatgatg catgtttact ggcatcgttg aagaaatagg
     3061 tgtcgttaaa agtgttcaaa ttcgtcaatc agtcaggacg attgaaattg aagcacataa
     3121 gattacggca gatatgcata ttggtgattc catcagtgtt aatggtgcat gtttaacagt
     3181 gattgatttt aatcagacat cttttactgt tcaagtaatt aaaggcactg aaaataaaac
     3241 ctatttagca gatgttaagc gacaatcaga agtaaattta gagcgtgcca tgagtggtaa
     3301 cggtaggttt ggtggacatt ttgtcctcgg tcatgtagat gaactaggaa cagtttcaaa
     3361 aataaatgaa acagccaatg caaaaattat tacgattcaa tgtagccaac atattaataa
     3421 tcagttagtt aagcaagggt ctattactgt ggatggtgtt agtctaacgg tatttgataa
     3481 gcatgataac agttttgaca ttcatcttat tccagaaacg aggcgttcaa cgattttatc
     3541 atccaaaaaa ttaggagata aagtacattt agaaacagac gttttgttta aatatgttga
     3601 aaatatttta aataaagata aagaccaatt atctgtagat aaattaagag catttggttt
     3661 ttaggagggg tagcatgcaa ttcgataata ttgacagtgc tttaatggct ttaaaaaatg
     3721 gagaaccaat tattgtagta gatgatgaga atcgtgaaaa tgaaggtgat ttagtagcgg
     3781 ttactgaatg gatgaacgat aataccatta attttatggc gaaagaagca aggggattaa
     3841 tatgcgcacc agtgtctaaa gatattgcac aacgtttgga tttggtacaa atggttgatg
     3901 ataactccga catctttggt acgcaattta cagtgagtat tgatcatgta gatacaacaa
     3961 caggaattag tgcttatgaa cgtacattga ctgccaaaaa gctcattgat cctagtagtg
     4021 aagctaaaga ttttaatcgt cctggtcatt tatttccatt agtagcacaa gataaaggcg
     4081 tattagctag aaatggacac acagaagcgg ctgttgattt agctaaactt actggtgcca
     4141 agcccgctgg tgtcatttgt gagattatga atgatgacgg cacgatggcg aaaggacaag
     4201 atttacaaaa gtttaaagaa aaacatcaat taaagatgat tacgattgat gatttaattg
     4261 aatatcgtaa aaaattagaa ccagaaattg aatttaaggc aaaagtgaaa atgcctacag
     4321 atttcggaac atttgatatg tatggtttta aagcgacata cacagatgaa gagatagttg
     4381 tactgacaaa aggtgcaatt cgacaacatg aaaatgtacg cttacattct gcgtgcctta
     4441 caggcgatat tttccatagt caacgttgtg attgtggtgc tcaacttgaa tcgtctatga
     4501 agtatatcaa tgaacatggt ggcatgatta tttatctacc tcaagaaggt cgtggcatag
     4561 gattgttaaa caaattacgc gcatatgaat taattgagca aggatatgat acagtaactg
     4621 caaatttagc attaggtttt gatgaagatt tgcgagatta tcatattgct gcacagattt
     4681 taaaatattt taacatcgaa catatcaatt tattaagtaa taatccaagt aaatttgagg
     4741 gattaaaaca atatggcatt gatattgcag aaagaattga agttatcgta ccagaaacgg
     4801 tacataatca tgattatatg gaaacgaaaa aaataaaaat gggtcattta atataggagg
     4861 attttaacat gaattttgaa ggtaaattaa ttggaaaaga tttgaaagtt gcaatcgtag
     4921 ttagtcgatt taatgatttt atcactggaa gattacttga aggtgcaaaa gatactttga
     4981 ttcgacatga tgttaatgaa gacaatattg atgtagcatt tgtgccaggt gcgtttgaaa
     5041 ttcctttagt agctaaaaaa ttagcctcat caggaaatta tgatgcagta attacattag
     5101 gatgcgtaat tcgcggtgct acgtctcatt atgattatgt ttgtaatgaa gtcgcgaaag
     5161 gtgtttctaa agtgaatgat caaactaatg taccagtcat atttggtatt ttaacgactg
     5221 aaagtattga acaagctgtg gaaagagcag gtacgaaagc tggcaataaa ggtgctgaag
     5281 cagcagtaag tgcaattgaa atggctaatt tattaaaatc tataaaagca tagagcactg
     5341 ttgtgagttc ataatcaaag atttataaat gtttcagaac tgccaatata acatttagga
     5401 gcctagaaca ttgattattg ttccagactc ctaaattatt gtttatgtct tgctctattt
     5461 acggcataat tttttaattg tacttaaacc taacataact gtagctccta aagctgcaat
     5521 tatgccaaca cgttttaagc cagcaggttt cacaaattct tttacagcaa gagataggtt
     5581 ttgtgggcgt tctgctaatc ttctcataaa atacgcaaac caatcatcgc cataaggtac
     5641 ataaatagtg aaattatagc cttcatttgc gatttcttct gctaactctg atctaaaacc
     5701 atagagcatt tggaattcca tacgatcttt ttcaatgtga ttttctttca taaattgttt
     5761 tacatgatta atgatgcgat ggtcatgtgt tgcaattgaa gtgaaattgc gtgcgtttaa
     5821 caaacgttgt tcaattattt tgatgtaatt tgcatctacg tcttccttag attgaaatgc
     5881 aattgattcg ttttctttat atgcaccttt aaccaaacgt aatcgtaaat cttggtactt
     5941 atcaactaat tcgtggctat cgtataaata tgcttgaatt acagtaccaa catttctaaa
     6001 ttcgcctttt aagcgatcta aaacttgaac aatttgttgc aggctagcat atttttcagt
     6061 atcaatattt atatgcatat tgttgtaagt atttgcttta agtaaaatct ctcttaaatt
     6121 ttggtaagct aattctaagt cgaattctgc acctaactga ctcaatttaa cagacatatg
     6181 tgcctttacg ccatgttgat gaagcgcgtc cataattgtt aaaatttgtt ctttagcatg
     6241 attactttct tcaactgtac cgacaaattc ccctaaattg tctaccgtaa cagcgatatt
     6301 cttgtcattt aagtattcga ttgtattaat taactctgga attgtatttc cggcaacgac
     6361 tttattggcg cccaaacgtg ggcccacttt ttttgctgcg ttgtttaaaa aactattatt
     6421 agataatccg ataaaaaaat tctttaatag tgccattgct actcctccaa acccttgtgt
     6481 ttacaataag tttagcatga aaaaaatatt cgagaaagcg ttttcatcaa aaacaatcaa
     6541 aaataattat gtataattac actattattg atgttgattt catttatata taataatatg
     6601 gacaacaata atactataaa tagaaatcta catagtgaat ttgctataat tatgttattg
     6661 aagattaatg aaaaaggggc agtataataa tgtggaagtg ggaagctgaa aatgatgcga
     6721 aaggcgtcgt tgttattgct cataatatat tagaacatac gggaagatat gcatatgtta
     6781 ttacgatgtt aagaagaaat ggctatcatg tgattatggg agatttgcct ggtcaaggtc
     6841 aaacttcaag agctaataaa ggacaaattg aaaactttca aacgtatcac gaaagtttgc
     6901 tagactggtt aaagatagct aatgaatata aaattcctac ttatgtatta ggggtaggac
     6961 ttggcggttt gattttatta aatttacttg aaaaagtaga attgccaatc gaggggatga
     7021 tgttaatttc tcctatgcta gaacttcaaa agaatggaaa aaatcgtaaa gataaacttg
     7081 tttcaaatat aggtaaaatt tcaaaagaca cacgttttaa tgttggtgta gaaccaaaag
     7141 atttaacacg taatttagaa attgttgaag aaacagttaa cgatggttta atgttaaaaa
     7201 aagcaacgta tcattggtat aatacaatta atgagacgat gaaagatacg atggctcata
     7261 ttcatgacat tcaaccaatg ccaacgctat taatgtatgg aacgaaggat ttaattgttg
     7321 atactagggc aattgatgag tttaaagaaa aatatcaaac acctgaatta tattttaaag
     7381 cttggcaagg tttttaccac gaggttcaca atgagccaga acgtgatgaa gtaatgcgtt
     7441 atattctgac tttcttaaat aacagcgtca atacaatggg ctttattgtt gaagatgacg
     7501 aaattgtaga aatttaatat tctaaaaagg
//
LOCUS       DAKTIP010000127         7477 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAKTIP010000127 DAKTIP010000000
VERSION     DAKTIP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.127"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(319..594)
                     /locus_tag="O0305_001950"
     CDS             complement(319..594)
                     /locus_tag="O0305_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCY0275090.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            735..1217
                     /locus_tag="O0305_001951"
     CDS             735..1217
                     /locus_tag="O0305_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275091.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            1219..1386
                     /locus_tag="O0305_001952"
     CDS             1219..1386
                     /locus_tag="O0305_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCY0275092.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(1526..3373)
                     /gene="typA"
                     /locus_tag="O0305_001953"
     CDS             complement(1526..3373)
                     /gene="typA"
                     /locus_tag="O0305_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCY0275093.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            3475..3666
                     /locus_tag="O0305_001954"
     CDS             3475..3666
                     /locus_tag="O0305_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCY0275094.1"
                     /translation="MKQKKSKNIFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(3820..4647)
                     /locus_tag="O0305_001955"
     CDS             complement(3820..4647)
                     /locus_tag="O0305_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCY0275095.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWIVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGLHQEISNDYLEPHHDALIQLHEQRFKRKSK"
     gene            4804..5418
                     /locus_tag="O0305_001956"
     CDS             4804..5418
                     /locus_tag="O0305_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCY0275096.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLRELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLIEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            5602..6954
                     /locus_tag="O0305_001957"
     CDS             5602..6954
                     /locus_tag="O0305_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCY0275097.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(7036..7455)
                     /locus_tag="O0305_001958"
     CDS             complement(7036..7455)
                     /locus_tag="O0305_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0275098.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGKVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         2493 a         1303 c         1093 g         2588 t
ORIGIN      
        1 ttcatatatt cagtcatcca attctacaat ttatacttca aatgatttcg ttttatactt
       61 taattttaaa aatccatgtt acttattata tacatgacac agtcataatc acaaatatta
      121 acaggtctct tgaggtaatt tatagaatat ttgtttactt aatatcgtga attgctaaaa
      181 cccaataaaa gtgactgaca tgtcatcaag aagacaacat aacagccact ttaatcagta
      241 attaaactat aaatataaaa tcggcgcagc tcaattgtcg atatctcacg ccattttaat
      301 atgcttatat attaaacctt aaacaagtgt aaaagcttca tgtaattttg aaagttcttt
      361 ctcaagacgt aacataattt gtttgccatc ttcgcggtca actaaaccta atttaacagc
      421 aaaatcaact tctttttgaa gtccaaacat ttgtgtatct agtacttctt catataatgg
      481 gcatgaaggt aatgttaaat tatctagttg aactttaatc agatgcaaga tttcatcagc
      541 atctttagtc aattgtttca aagctgcatt tttcattgtt gcttgtttcg ccatgacacg
      601 ctccccctct atataaattc tcaagtctta ctaaaagttt atcttactaa atattaaaaa
      661 gcaagtagat ttaaaatgtg tcgttagcaa tttgtgttaa aataatgcta tatacattga
      721 tagggagtgg gaaaatgata caaattaaag gagccgtaaa gtttcctata acattagata
      781 gtacgacttg gattttcgat gatagaaaag ttagcatcga agatttagaa aaaggtgtat
      841 ttgatggttc aaaaccaatc gactttgaag ataatcatga atggaatcgt gctatcctag
      901 aaggacaaac taatccgcct actttaaatt cagaaataaa atataaaaaa cgtgccatgt
      961 tagaaggttc atttgtcatt aatatgacac catttttcaa acattccgaa ccacatgact
     1021 ctgcaactac tattcgatta tcaaatgagc atgatcattt agatataccg atagatctat
     1081 taccctattt atttttccaa tttgcaaaag acggtaaacg actttacagc gataatggtg
     1141 tagatagttt tatttacaat caagaagatg gttattcata tcgaatcaac catgtaactc
     1201 atattgaggt gatttaattt gagacaagtg caatgcatta tctgtgatgc aaaagtattt
     1261 atagatgagc gcacaactga gtctaaacga cttaaaaaca atcctattcg aacatttatg
     1321 tgtgatgatt gtaaaagtcg attagacaca cctaaacaac gtgcgcaaca ctatccgctc
     1381 gattaatatc tgaataaaca agttagaaat ttgaaaatgt gaaagtaaca ggaaataata
     1441 gcatttaaac actttttcaa atattatatg ccaaaagaga agctaagcaa ctttcatcgc
     1501 tcagcttctc ttttattatg ttaaattatt cgttttcttg catcatttgt ttgatacgct
     1561 ttgcttcttt ttcacgaaca tttttgttta aaattttctt tcttaaacgt atactttctg
     1621 gtgtaacctc aacaagttca tcatcattaa tgaattgtaa cgcttcttcc aatgttagaa
     1681 tacgcggtct attcattgtt tgtgtttggt ctttcgttgc agaacgtacg ttagtttgat
     1741 gttttgtttt agtgatgtta acagttaaat cattttcacg attatgttca ccaacaatca
     1801 taccttcata aacttcagta ccaggttcca tgaagtttac acctctatct tcaagtccca
     1861 aaatggcata agtacttgca gaaccttgat ccattgaaat taatgcacca ttacgacgac
     1921 cgccaatttg tgctttaata cgtggtctaa attcttcaaa tgtatggtta ataataccgt
     1981 aacctcttgt cattgacata aattcagtcg tataaccaat cataccacga gccggtacat
     2041 taaagattaa acgtgtaagt ccattatcag ttgtagtcat atcaaccatt tcacctttac
     2101 gtgcacctaa tgattcaata acagcacctg cattttcttg tggcacttca cattgcacac
     2161 gttcaaatgg ttcacacatt acaccatcta tttctttaat aattacttgt ggttttgaaa
     2221 cttgtaattc ataaccttca cgacgcatat tttcaataag gattgataaa tgcaattcac
     2281 cgcgaccagc aactacccat gtatctggag aatctgtgtt agaaactttc aaagatacat
     2341 ctgtttctaa ttgttgattt aaacgttctt gaatttgacg tgctgttaca aagtcacctt
     2401 cacggccagc aaatggagaa ttgttaactt taaatgtcat ttcaagagta ggctcatcaa
     2461 tacgtagaac tggcaatgct tcttgatggt catgtggtgt tacagtttca ccaacattaa
     2521 tgtcttccat acctgaaaca gcaattaaat ctccagcttg tgcttcttca atttctaaac
     2581 gttttaatcc aaagtaacca aagattttag ttacacggaa gtttttcact gtaccgtcta
     2641 atttaattag tgatacatta tctccgacac gcattttacc tctgaataca cgaccaatac
     2701 caatacgtcc aacataatca ttgtagtcca acaatgctac ttggaattgt aatggctcat
     2761 cactgttatc aattggagct ggtacataat caataattgt ttcatataat gattgtaaat
     2821 tatcatcttg cttttcagga tctaagctag ctgtaccatt tactgctgaa gcataaacaa
     2881 cagggaattc taattgttca tcgtttgctt ctaattcaat aaataaatct aaaacttcat
     2941 ctacaacacc ctctggacgt gctgatggtt tatcaatttt attaacaaca acaacaggtt
     3001 tcaggttttg ttctagcgct tttttaagta caaaacgtgt ttgaggcatt gtaccttcat
     3061 acgcatctac tactaagaca accccatcaa ccattttcat aatacgttct acttctccac
     3121 caaagtctgc atgtcctggt gtatccaaaa tattaatacg tgtaccttta taatcaacag
     3181 ccgtattttt ggctagaatc gtaattccac gctctctttc gatatcgtta gagtccattg
     3241 cacgttcatc gacatgttca ttttctctga atataccaga ttgttttaac aactcatcta
     3301 ctaaagttgt tttaccatgg tcaacgtgag caataattgc tatattgcgg acatcttctc
     3361 ttttattagt catataaata gtttccttct ttcttaagta aaatcccact tttaattaca
     3421 acttttttat tatatcatat aattgagaaa agataagcaa aaggtgggga taggatgaaa
     3481 caaaaaaaat ctaaaaatat cttttgggta ttttctatat tagctgttgt tttcttagta
     3541 ttatttagtt ttgctgttgg tgcatcaaat gtaccaatga tgattttaac atttatatta
     3601 ctcgttgcaa cctttggaat tggatttact acaaagaaaa aatatcgaga aaacgattgg
     3661 ctataagtta tatcgacaat aacacgcgtt gatttaagac cacacatgtt catgatgaac
     3721 atttaactgg ttttatattg gcgcgtgttt ttattattta aggtataaaa gcgtaatatg
     3781 ttacctaatt gttaggacta catattacgc ttttggtaat tattttgatt ttcttttaaa
     3841 tcgttgttca tgtaattgta ttaacgcatc atggtggggc tctaaataat cattgctaat
     3901 ttcttgatgg agaccacgat ttccaactaa gattgaattt ggaccactaa ttgttaatgg
     3961 ttctcctagt aaattggaag cttgtccatt tacttcatat aaaataacca atccgccagc
     4021 aaaatcccat ggttgaagtc ttggcgtcat atatgcttct aaattacctg tagcaactga
     4081 aacgatttca agcgctgcac taccatatgc ccttgcactt ctagaatcat taacaatttc
     4141 tttaaagatt tctcctaaaa ttggtttagt taaccagttc ggattgatcc caataatgct
     4201 ttgtcttaga ttagaatcat tcaatggttt caagggttgg ctaccacgat atgcaccttc
     4261 ccctacttta gcatgatata agacatcagc cataacatca tatacaaaac ctgcataagg
     4321 tttaccatcg atataaatac caattgaaat tgcgaaattt tcttgttgat gaacaaaatt
     4381 caatgtaccg tctattgggt caacaatcca taccgtacct ttggaagtat cgatgtcatg
     4441 accatgccct tcttcaccta atacttgatg attgggatat gtttctaaaa ttgtatcaaa
     4501 aatgaaatct tctgttgctt tatctacatt tgtaacaagg tcattcggat ttgactttgt
     4561 ttcaattgtt aaattttgct ccatcaattg tttaattcta attcctgctt cttgaataag
     4621 tccttgggca aatccataaa gtgccataac atcatctcct aacatcttta ttatacatca
     4681 acattttata aaaataactt cttatgataa aaatgaaaat actaatttta atgagagcgt
     4741 cttaagccgc aattgataaa acatatgcta caatattttt aaacactata caggaggtac
     4801 tctatgacaa aatatacatt taaacctaaa gatttcaaag cgttcaacgt agaaggctta
     4861 gacgcacgaa tggaagcttt aaacgaatac atacgaccac aactccgtga attaggagaa
     4921 tattttagtg acttctttac aagtcaaact ggtgaaacat tttatcctca cgtagcaaag
     4981 catgctagaa gaagtgtgaa tcctcctaaa gatacatggg ttgcttttgc aacaaacaaa
     5041 agaggctata aaatgttacc tcatttccaa attggtatgt ttgaagatca actgtttgtt
     5101 atgtttggaa tcatgcatga agcaaaagat aaagcaacac gtgcaaaagt ttttgaaaga
     5161 aaatttaaag ctattcaaca attacctgat gattatcgtg tttgcttaga tcatatgaaa
     5221 cctgataaac catttattaa agatttaacg gatgatgatt taatagaagc gatacaaaga
     5281 gccatcaatg tgaaaaaagg tgaattcttt atagcgcgtg caatcacacc acaagataaa
     5341 agattaaaaa gtgacaaagc atttattgca tttttagaag aaaccttcga tcagttctta
     5401 ccattttatt ctgcataaat aactttgttt aaataataga gcacttaatc acatccatga
     5461 tttcgtgctc ttttttctta atattaaatc gaacgttcaa cataataatt catactttta
     5521 aaaaaattaa aataaattta ggttgaccta aacattttat taggttatta tattgtccat
     5581 aagaagtaga ggtgagtcaa aatgaataat aaacgacatt caacaaatga acaattaagt
     5641 ttagacgaaa taaacaatac aattaaattc gatcatcgca gttcaaataa acagaaattt
     5701 ttatcatttc ttggacctgg gttattagtc gctgttggtt acatggatcc cggaaactgg
     5761 ataacatcaa tgcaaggtgg cgcccaatat ggctatactt tgctattcgt aattcttatt
     5821 tcaagtttat cagcaatgtt acttcaaagc atgacagtga gattaggaat agcaacaggt
     5881 atggacttag ctcaaatgac acgacattat ttatcaagac ctattgctat aatcttttgg
     5941 atcattgcag aactagcaat tatcgctaca gatattgctg aggttattgg tagtgctatt
     6001 gctcttaatc tcctatttaa cataccttta atcgtcggtg cactaataac tgtacttgat
     6061 gtatttttac tactttttat aatgaaatat ggttttagaa aaattgaagc tattgttggt
     6121 acattaattt tcacagtgtt attcatcttt atatttgaag tctatatttc atcaccacag
     6181 ttgaatgctg tgttaaatgg atttatacca catagtgaaa tcattacaaa taacggcatt
     6241 ctctatattg cattaggtat tattggcgct acaattatgc ctcataattt gtacttacat
     6301 tcatcaattg tacaatctag aacatactca agacataaca atgaagaaaa agcgcaagcg
     6361 attaaatttg ctacgataga ttcgaacatt cagttatcaa tcgcatttgt agtcaattgc
     6421 ttattattag tgttaggagc atcactattt ttcaactcaa atgctgacga tttaggtggt
     6481 ttctatgatt tatatcacgc cttaaaaact gaacctgtac taggtgcaac aatgggtgca
     6541 atcatgagta cattatttgc agttgcatta ttagcgtcag gtcaaaattc aacgattact
     6601 ggtactttag caggacaaat tgtaatggaa ggatttttaa gattacacat accaaattgg
     6661 ttaagacgtt taattacacg ttctcttgct gtcattcctg ttatcgtatg cttaatcatt
     6721 tttaaaggta atgcagctaa aattgagcaa ctacttgtat tctctcaagt atttttaagt
     6781 attgctctgc cattctgttt aataccatta caattggcta ctagtaataa agacttgatg
     6841 ggacctttct ataataaaac atgggttaat atcatttcat ggacacttat tattattcta
     6901 agtattttaa atgtctattt aatcgttcaa acattccaag aactacaaag ttaaaaataa
     6961 aaccattaaa atgttggttg tatctaataa ttaacaactt aacattttaa tggtttttct
     7021 tttgctatga acatattaat gataaatata ctcatcgtcc ttatgtgttg tttcttttga
     7081 ttctttttta gaaatcaaca taatcatcac gataacccat aagacaattg caattaaatt
     7141 aagagccatc aaacttacaa ttatcgcgat aacaaaaagt ataatcgata attttctacg
     7201 tcctttaata caaattgtcg caaatatacc ataaagtgtt gaaattaata aaataattgc
     7261 taatgcaccc attgttgtct gaagcatatc taaagaaacc ttgccaccgt tctgagataa
     7321 ttgcttcgct aattcttcac gttgtgatgc attaccactt tttaaagaaa aataggatag
     7381 ccccataact aataaataaa taatactaat actatttgca atccaagcca tgattaattc
     7441 aggttttcta ttcatcttaa aatcctttct ttgttta
//
LOCUS       DAKTIP010000128         7432 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAKTIP010000128 DAKTIP010000000
VERSION     DAKTIP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.128"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..331
                     /locus_tag="O0305_001959"
     CDS             <1..331
                     /locus_tag="O0305_001959"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0275099.1"
                     /translation="IFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIYYPTSKGETTNNL
                     DLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITSFEPESFDQPVDK
                     LIIYQAA"
     gene            343..1806
                     /locus_tag="O0305_001960"
     CDS             343..1806
                     /locus_tag="O0305_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCY0275100.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPAT
                     YTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHI
                     LMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLI
                     GSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGY
                     YYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIVKQ
                     TINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLND
                     DEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
     gene            1787..1990
                     /locus_tag="O0305_001961"
     CDS             1787..1990
                     /locus_tag="O0305_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCY0275101.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            1987..2973
                     /locus_tag="O0305_001962"
     CDS             1987..2973
                     /locus_tag="O0305_001962"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCY0275102.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFAPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            2973..3302
                     /locus_tag="O0305_001963"
     CDS             2973..3302
                     /locus_tag="O0305_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCY0275103.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            3299..3922
                     /locus_tag="O0305_001964"
     CDS             3299..3922
                     /locus_tag="O0305_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCY0275104.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            3974..4948
                     /locus_tag="O0305_001965"
     CDS             3974..4948
                     /locus_tag="O0305_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCY0275105.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI"
     gene            4920..5990
                     /locus_tag="O0305_001966"
     CDS             4920..5990
                     /locus_tag="O0305_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCY0275106.1"
                     /translation="MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            6004..6315
                     /locus_tag="O0305_001967"
     CDS             6004..6315
                     /locus_tag="O0305_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0275107.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            6293..6739
                     /locus_tag="O0305_001968"
     CDS             6293..6739
                     /locus_tag="O0305_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCY0275108.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYENSDTIKVIENNNIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIYNNNSIYRIIFHIEKGRIRYEKL"
     gene            6726..7025
                     /locus_tag="O0305_001969"
     CDS             6726..7025
                     /locus_tag="O0305_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCY0275109.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            6943..>7432
                     /locus_tag="O0305_001970"
     CDS             6943..>7432
                     /locus_tag="O0305_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCY0275110.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDILDDFKGVDRNDIEIRQHRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTI"
BASE COUNT         2761 a          929 c         1281 g         2461 t
ORIGIN      
        1 tatttttgta ccgaaaatgg attatttaaa tcatcaaatg acttttaaag agatatttaa
       61 tctcaaagat attgatgtcg ataataaggg gatttactat ccaacttcaa aaggtgaaac
      121 aacgaataac ctagatttaa ttgttgttcc tggtgttgga tttcaagacg atggatatag
      181 aattgggtat ggtggtggct attacgacag gtttttagct aattatcaga caaagacaat
      241 aagcttatta tacgattttc aaataacatc atttgaaccg gaatcattcg atcaaccagt
      301 cgataaattg attatatatc aagcagcata gtggaggact aaatgaacat agacaaacaa
      361 ttttggaaaa caatatatta ttggattagg tacttgaatt ttgatattgt cagtagggaa
      421 aaagatgacc aagaaatttg gttagcccat aaaagaaaaa aacaagtagt catttttaaa
      481 caacatatca agtctaccca agaaatacgt tttgacaaag cgaaagtgct tgaacacaaa
      541 gatgaaatag caaattttat ttctttcgaa ccacaaagtt ttgaatttta ttattttaca
      601 gaatcggaat tttcagaaga acaattaaat gaagtttcgc caattagaat taaattcaat
      661 gttataagac acacaaaaga tttgataaag catatgccga atatattttt ggctagactt
      721 atttcagaag ataatgataa aaagacatat atgttctata aacgcaaagt attaaccgat
      781 aactttttag ataaatatat gcagaaattt tcaccggcaa catacacaat aatatttgta
      841 aatgtcttaa tatggttatg tatgatttta tatttaaata atttttcgga tgtaaaatta
      901 ttagatgttg gcgggttagt gcattttaat gtcgtacatg gtgaatggta tcgaattgtt
      961 acatcgatgt ttttacattt tagttttgaa catatactta tgaatatgct ttcattattt
     1021 atttttggta aaatagtcga agcaattatt ggttcatggc ggatgttaac tgtatacttt
     1081 attgcagggt tgtttggaaa ctttgtatca ctatcattta atacgactac aatttcagtt
     1141 ggggctagtg gtgctatatt tggtctgatt ggatcaattt ttgcgatgat gtatgtttca
     1201 aaaacattta acaaaaaaat gttaggacag ttattaattg cattagtgat attagttggt
     1261 gtttctctgt ttatgtcaaa tataaatatt gtggcgcata ttggaggatt cattggtggt
     1321 ttattaataa ctttaattgg ctattactat aaagtgaatc gtaatatttt ttggatttta
     1381 ctaattggta tgcttgttat atttattgca cttcaaatta gaatttttac aattaaagaa
     1441 gataatattt ataataaatt gatcaaagat gatatgacta gtggtaatta tgataatgct
     1501 caaaatattg tgaagcagac aataaataaa aattatgccg atgatcaaac atattattta
     1561 agtggtatga ttatggctac tatcaattct aagtccgaag gtatgacaga atgggagcga
     1621 ggacttagaa tgtttcctaa atcaggttta ttaaattttg agttagcgat agcaaatcgt
     1681 tcattaaatg atgatgaaaa agcattaaaa tatgtgcgta aagcattaaa tgcagaccct
     1741 aaaaatacag attatattaa cttagaaaaa gagttgacta aatcaaatga gtcgaaaaat
     1801 aaataacttt tatgatgtac aacagttatt gaaaagttac ggatttctaa tatattttaa
     1861 aaatccagaa gatatgtacg aaatgattca acaggagatt tcatcattgt atcaatatga
     1921 actgttatct aaggaagaat atttgaaatg tacgttgata attaatcaga gaaggaatga
     1981 acagaaatga caaaaattat tttagcagct gatgtaggcg ggacgacttg taaattaggt
     2041 attttcacac ctgaattaga acaattacat aaatggtcta ttcacactga tacatctgat
     2101 agtacaggat atacactttt gaaaggaatt tatgattcgt ttgttgaaaa agtaaatgaa
     2161 aataattata atttttcaaa tgtacttggc gtaggtattg gtgtaccagg tcctgttgac
     2221 tttgaaaaag gtacagtaaa tggagcagta aacttatatt ggccagaaaa agttaatgta
     2281 cgtgagattt ttgaacaatt cgttgattgt ccagtgtatg tagataatga tgctaacata
     2341 gctgctttag gggagaaaca caaaggtgct ggtgaaggtg ccgatgatgt tgttgccatc
     2401 acacttggta caggtctagg tggaggaatt atttcaaatg gtgaaatcgt acatggtcat
     2461 aatggctctg gcgcagaaat aggtcatttt agagcagact tcgatcaacg atttaaatgt
     2521 aattgtggtc gttctggatg tattgaaaca gttgcttcag cgacaggcgt tgttaactta
     2581 gttaacttct actatccgaa gttgacgttt agatcttcta tattagaatt gattaaagaa
     2641 aataaggtta cagcaaaagc tgtttttgat gcggcaaaag ctggtgacca attctgtatt
     2701 ttcattactg aaaaggttgc aaactatatt ggatatttat gtagtattat tagtgttaca
     2761 agtaatccga aatatatcgt tctaggtgga ggaatgtcta ctgcaggacc tattttaatt
     2821 gaaaatatta aaacagaata tcataattta acatttgcac ctgctcaatt tgaaactgaa
     2881 attgtacaag cgaaattagg taatgatgca ggtattacag gagcagcagg attaatcaag
     2941 acctatgtat tagataaaga gggggtaaaa taatggctat tgttgatgtg gttgttattc
     3001 cagttggaac ggaaggtccg agtgttagta aatatattgc agatattcag aaaaaacttc
     3061 aagaatataa agcaatgggt aaaattgatt ttcaattaac accaatgaat actctaattg
     3121 aaggtgaatt aagcgatgta ttagaagttg tgcaagtgat acatgaatta ccttttgata
     3181 aaggtttaag tagagtttgt acaaatatcc gtattgatga ccgacgagac aaatctagaa
     3241 aaatgaatga taaactaaca tcagtacaaa aacatttaga aaatagtggt gaaaacctat
     3301 gaggatttca agcttaactt taggcttagt tgatactaat acgtatttca tcgaaaatga
     3361 caaagctgtt attctgattg acccttcagg tgaaagtgaa aaaattatta aaaaattaaa
     3421 ccaaataaat aaaccgttaa aagctatttt attaacacat gcacactttg atcatatcgg
     3481 agcagtcgat gatatagttg atcgattcga tgtcccggtt tatatgcatg aagcagagtt
     3541 tgattttcta aaagatcccg ttaaaaatgg ggcagataaa tttaagcaat atggattacc
     3601 aattattaca agtaaggtaa ctcctgaaaa gttaaacgaa ggtagcacag aaatagaagg
     3661 atttaagttt aatgtgttac acacacctgg acattcacca ggaagtttaa catatgtgtt
     3721 cgatgaattc gcagttgttg gagatacatt atttaataat ggaatcggac gtacagattt
     3781 atataaaggt gattatgaaa cgctagttga ttctattcaa gataaaatat ttgaattaga
     3841 aggcgattta cctttattcc ctggacatgg tccatatacg acggttgatg atgaacaatt
     3901 aaatccattt ttacacggtt aaattgaagt aataaaaaca cctcctacgt gattatatgt
     3961 aggaggtgtt tttttgaaga ttctatttca agaaataatt aataaagcga tagaaatgaa
     4021 agcgagtgat gtacatttta ttccagttaa aaatgaagta agtattaaat ttagaattaa
     4081 tgataacttg gagcagtatg aacaaattgg gaatagcatt tatcaaaagt tattagttta
     4141 tatgaagttt caagctgggc ttgatgtttc tacacagcaa gtcgcacaga gcggtcgata
     4201 tagttacctt ttcaataaaa tatatttttt gagaatatca actttaccat tgtcacttgg
     4261 ccaagaaagt tgtgttatca gaattgtacc tcaatttttt caaccacaga aatcaactta
     4321 taaattcaat gattttaaac acctcatgaa taagaaacaa ggattactat tgtttagtgg
     4381 accaactggt tcaggaaaga gtacattaat gtatcaaatg gtctcatacg cgaataaagc
     4441 cttgaattta aatgtaattt ctatagagga tcctgtagag atgcaaattc ctggtatcgt
     4501 ccaaattaat gtgaatgata aagctggcat aaactatgta aattcgttta aagctatttt
     4561 aagatgtgat cctgatgtta ttttaatagg tgaaatcaga gataaagatg ttgccaagtg
     4621 tgttatacag gctagtttaa gtggtcacct tgttctgact acattgcatg caactgattg
     4681 taaaggtgct attttaaggc tattagaaat gggcatttct gtacaagaat tgatacaggc
     4741 aactaactta attataaacc aacgacttgt aactactatt aagcaacagc gacaattagt
     4801 atgtgaaatt ctatctcagc aacaactccg atatttcttt tcccataatc attcattacc
     4861 atcatcattt aagaacttag aagataaact tgatgatatg acaaaagcag gtgtcatttg
     4921 tgaaactaca atgcataaat acatttaaac tacattctaa gaagcgacaa ttaagtaagg
     4981 cccaacaaat cgacttactt tcaaatttat gtaatttgtt gaaatatggt ttcactctgt
     5041 atcaaagttt tcaattttta aatcttcaaa tgacatataa aaataagcaa ttaggtacca
     5101 ccattctaag tgaaatttca aatggtgcac catgcaatca aatattatca ctgataggtt
     5161 atagcgatac tatcgtcatg caagtatatt tggcagaaag atttggcaat attatagacg
     5221 ttctagaaga aaccgtaaat tatatgaaag tgaatagaaa gtcagaacaa cgattgttaa
     5281 agacactgca atacccctta atactagttt ctatctttat tgctatgatt attatattaa
     5341 acctcacagt aattccacag tttcaacaat tatatacttc tatgaatatt caactatctt
     5401 cttttcaaaa aacattgtct tttttcatta ccagcttacc tactataatt gtagtaatgc
     5461 tcataatagt atctatgttg gctattatta tgaaattaat ttataacaat ttaaatatgc
     5521 tcaataagat aaactttgtg atgaaactac cgctaatatc aggctatttc caattattta
     5581 aaacttattt tgtaactaat gaattagtgt tgttttataa aaatggtatt acacttcaat
     5641 caatagtaga cgtttatatt aaccatagta gtgatccatt tagacagttt ctaggtaaat
     5701 acttattaac ttattcagaa atgggatatg gtttgcctca aattttagaa aaactaaaat
     5761 gctttaagcc tcaattaatt aagtttgtgc tacaaggtga aaagagaggg aagctagaag
     5821 tagaactaaa gttatattcg caaatattag taaaacaaat agaagataaa gcgataaaac
     5881 agactcagtt tttacagcct attttatttt tgattttagg tttatttatt gtcgcaattt
     5941 atttagtaat tatgttacca atgtttcaaa tgatgcaaag tataaaataa tggaggttaa
     6001 tatatgttta aatttcttaa gaaaactcaa gcgtttacat tgatagagat gctattagtg
     6061 ttattaatca tcagtttatt attaatttta atcattccaa atattgctaa acaaactgct
     6121 cacatacaat caacaggttg taatgcacag gtaaaaatgg ttaatagtca aattgaagcg
     6181 tatgcattga aacataatag aaatccatcg tctattgaag acttaattgc agatggtttt
     6241 ataaaagaag cacaaaagac atgtaaatca ggagagacaa tcacaattag taatggagaa
     6301 gcagttgcaa attagaaagc agtcagcatt tactatgatt gagatgcttg tggtaatgat
     6361 gttaatcagt atatttctac ttttgacaat gacatctaaa ggattaagca atcttagagt
     6421 aatagatgat gaggcaaata tcatttcttt tattactgaa ttgaattata ttaagtcgca
     6481 agctatagca aatcaaggat atatcaatgt tagattttat gaaaacagtg acactattaa
     6541 agtaatagag aataataata tacgatttct aaaattaaaa gtaggcaaaa taattaatgt
     6601 tgcaaaagtt gatattattg cctttgataa aaaagggaat atcaataaat ttggtagcat
     6661 aacaatttac aataacaatt caatttatag aataatattc catattgaaa aaggaagaat
     6721 tcgttatgaa aagctataag tgtaaaggtt cattcttaat agatagtatg gctggatttt
     6781 tgctaattgg attgattaca ttactattga taccaatgat gaatcaaatg caagcgagta
     6841 taaaccataa actacaaaca attgatgctt ctaaagtaat tttgacgact gtatctaaaa
     6901 ttaataaaga agaacttaag aagggggtaa ctatagggaa gtatgatatt aagcaaagtg
     6961 accaacaaat ttgtgctatt tcaaaaaata ccacttctta tcaaaagaca tgtatacagt
     7021 attaatgtca aagctttttc gctcattgaa atgttagtag cgatgatggt tataagtata
     7081 actttactaa ttgttccaga cttaattaga cttagtaaaa cttttctaat tgaaagtagg
     7141 gatttaacaa ctgtagattt cgaatttttc tcaagagata ttctagatga ttttaaagga
     7201 gtagatagaa acgatattga aattaggcaa caccgtatca ttttacataa aggtgaagaa
     7261 atgatcgaat acaaattaat taataataaa attattaaag ttgtaaatga cagaggaaat
     7321 ataacaatga ttaataatgt tacagcattt actgcaaata tctactataa atccattatt
     7381 aaaataacga taacagttaa agtcggtaca aatgtgcaga ctaaaactat tt
//
LOCUS       DAKTIP010000129         7387 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.129, whole genome shotgun sequence.
ACCESSION   DAKTIP010000129 DAKTIP010000000
VERSION     DAKTIP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.129"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(277..1740)
                     /locus_tag="O0305_001971"
     CDS             complement(277..1740)
                     /locus_tag="O0305_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCY0275111.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLAAAEELNLLGYQVTIYERARESGGLLMCGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            complement(1758..6257)
                     /gene="gltB"
                     /locus_tag="O0305_001972"
     CDS             complement(1758..6257)
                     /gene="gltB"
                     /locus_tag="O0305_001972"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCY0275112.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            6438..7322
                     /locus_tag="O0305_001973"
     CDS             6438..7322
                     /locus_tag="O0305_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0275113.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
BASE COUNT         2253 a         1630 c         1069 g         2435 t
ORIGIN      
        1 attcctccta aacacaatat ttatcatata tgaacgttct gtaaaagtca atattgtttt
       61 taagcattac atgattacca caaatcattg tcttccacta cagttgacct ataaacgaat
      121 gtaaaaaatc actacttata attaatctat ttgttgtctt acaaaccttt gtttttaggt
      181 tacaaagatg gcatgtgacg ttgatagata ctagattttt tcaaactcga ttcagcatgt
      241 cacaacgtca acgtaaccac catttccata caaagattat acacaaactt tactagctaa
      301 atactgatct actgctttcg ctacgcctct accttcttta attgcccata caactaaact
      361 ttgaccacgt ctagcatctc cagcagcaaa taccttttca ttattagttt gatagtttgt
      421 atcatccgcc acgattcgat ttctatccgt tttaatgtta aaagcattcg gtactgttgg
      481 ttctgtacct tcgaagccga ttgataataa tacaaggtca gcaggccaaa atctttcagg
      541 tccttctttc atgaccatac cattttcgcc ttgctctaaa atttgagtat acaaaccacg
      601 tatgtgtcct ttatcgtcaa catcgtaacg cattgtttga acaccatatg cacgtggttc
      661 cttaccaaac ttagcttcgt actcttggtg cgcatagtcc attttaaaca ccggcattgc
      721 taaaggccat gatgcatttt ctgtaaatgt aattgcttct ggcaatttcg tatatttatt
      781 aaattgaaca atcgatttac aattttctct taatgctgtc gctacacagt ctgcccctgt
      841 atcaccagca ccaatgataa tgacattctt atcttttgca gttattgtta tatcatcaat
      901 ttctccattt aacaactgcg tttgttcagt taaataatcc atagcgaaat gtataccatc
      961 acccatgcgt ccttctaaag gtaaatctct acctttttgt gcaccagtac ataatataat
     1021 ggcatcatac tcagattcta acgttgcttt atcaatatca acgccgactt caacaccatt
     1081 aatgaaagta atgcccgctt cttccattaa cttaatacga cgtcgaacca catctttatc
     1141 aagtttcata ttcggaatac cacacattaa taaaccgcct gattctctag cacgttcata
     1201 aatagttact tgatatccta gtagattaag ttcttcagca gcagctaatc ctgctggacc
     1261 gcttccaacg attgccactt tttcatctct acggcgactc ggaactttcg gcgctaccca
     1321 accattttca aaagcttcat caataattgt gcgttcaata cctttaatcg caatcgattc
     1381 tctattaatc ttcatcacac aagcactttc gcatggtgca ggacatacac gccctgtaaa
     1441 gtcaggaaag ttatttgttt cgcttaagcg ttcataagca gttttaaaat cttgatgata
     1501 cactaagtcg ttccattcag gaatgtagtt tccaattgga caacctattg tttccctacc
     1561 atactgttgt ccggtttgac aaaacggcgt tccacaatcc atacatcgtg caccttggat
     1621 agaggcatct tctttagtaa atcgttgttg atatgcttta tgatgcttca aacggtctac
     1681 cagtgataat tcacctaagt actgtttgtc atacttcata aatcctttaa attcacccat
     1741 cgtatctccc cctttcctta atacacaacg gctggtttat gtttagcatc gattgtttta
     1801 ctgtcatcgt aaaatgcagc taacatcgct tcatcttcat tgtcatgtaa tgatttgtgc
     1861 aaatgaattt tttgcatcat taattgataa tctttaggaa taactttaac gacgacatct
     1921 tcaatgcgat caaaatgttt taacacatga atcgctctcg tactattcgt gtgtgacaca
     1981 tgttcttcca gcatttgctt aatgaatgct ttttcttctt ggtgtttaat ctttgtaaac
     2041 gaaagcgtat ctagttgatt attttcaaca aaagcttcta catcagacgg gataacgtaa
     2101 gcaataccac cactcatacc ttgaccgaag ttcttaccta catcacctaa attaatgaca
     2161 tgtccaccag tcatatactc taatccatgg tcgccgatac cttcaacgac aacatctaca
     2221 ccactatttc taatacagaa tctttctcct gcactaccgt taataaatgc cttaccactt
     2281 gtcgcaccat agaatgagac gttaccagca ataatttcat tttgtcgttc ttcaaaaggt
     2341 gctttgacaa tgaccgtacc accagataat cctttaccaa catagtcatt cgcatctcca
     2401 gtatgatgaa tcattaagcc tttcggtgca tatgctgcaa gactttgacc agcatgacca
     2461 ttcgtataaa cattaattgt attttcagga agtcctgctt ctccatattg tttcgaaatc
     2521 tcactacctg taataacccc tacatcacgt tgttcattat ttactgtaaa gctacctgta
     2581 tagcgacgcc cttcagcaat atatggcttc gttacttcat ataaatttgt taaatcaaat
     2641 ccatgctcaa gattatgatt ttgttgaatt tcttttgtgt ttggcccatc gaaaggacat
     2701 aacagttttt caacatcaat actagccgct ttgctattcg cttttaatgt tgatgatcgt
     2761 tgtaataaat cagttcttcc aactaagtct tctacacgtt tcaaacctaa agatgctaaa
     2821 atttctctta attcttgtgc aataaaatgc ataaaattaa caacatgatg tgctttacct
     2881 ctatataaag cacgtaaatc tttgttttga gttgcaactc ctactggaca tgtatcttta
     2941 tggcatacac gcatcataat acagcccaac accactaatg gtgcagttgc aaatccaaat
     3001 tcttccgctc caagcgcaca tgcgtacgct acatctttac cagttaataa cttaccgtct
     3061 gtttctaact taacacgact tcttaagtca tttagtttta atgtttgatg tgtttctgct
     3121 aaaccaatct cccaaggaac accggcatgc tgaatactcg ttttgggtga agcccctgta
     3181 ccaccatcgt aaccactgat gacaatttta tctgcaaatg cttttgccac cccagatgca
     3241 atggtaccaa cacctgtttt cgaaactaat tttaccgcga tatctgcatc tttattcgca
     3301 tttttcaaat catgtatcag ttgcgctaaa tcttctattg aataaatatc atgatgtggc
     3361 ggtggtgaaa tcagaccgat acctggcgtt gaccctcttg tcttcgcaat ccacggatat
     3421 accttagtac caggtaattg accaccttca ccaggctttg caccttgcgc aactttaatt
     3481 tgaatttctt tggcatgttg taaataatca ctagttacac caaaacgccc agaagcaact
     3541 tgtttaatcg cacttacttt gttgcttcca tcaacttgta cttcataacg ttttgcatct
     3601 tcgccacctt caccactatt actctttcca cctaattggt tcatggcttg tgctaacgtt
     3661 tcatgtgctt ccgctgaaat cgatccataa ctcatcgccc ctgtattaaa gcgtttgaca
     3721 atgtcactta ccggttcaac ttggtcgatg tcaatcggtg tacatgcttt aaattcaagt
     3781 aaatgtctaa tgtgatctgt tctatttttg ttcaccgctt cagagtatgc tttaaattgc
     3841 gcatagtcat tttctttaca tgcgtgctgc aataagaaaa tagattccgg attaaaagca
     3901 tgatgttgac cttgttgtct ccattggaat gtactacctg atgcaagata attatcatca
     3961 ctttgttgac gtgctttatt ttcagcatca atttgatcaa tcgaaatacc agataactta
     4021 gactgtgtcc cagtaaaata acgatcaatc acatcatgag acaagccaat cgcttcaaat
     4081 atttgtgccc cttgataact ttgcactgtc gaaattccca tcttagccat tactttaatg
     4141 acaccttctg acaatacatc cgtatatgtc ttaacattat cgacaacggt gccttgtaac
     4201 ccttctgtca atgtcagttg ttcaactgta cgttgcgcta ggtatggcac aattgcattc
     4261 gcgccatatg cgagtaaaca agcaacatga tgcacttctc gtgtctcacc agatttagcg
     4321 actaaacttg tagacatacg taaatctgct ttaataagta attgatgcac atgacttatt
     4381 gcgagtaaca tcggcattgc aaagccattg ctatcaacta atccactatc atctaacact
     4441 agaatttgag cgccttgctt tacagcattc actgcttctc ggcctaatgc ttctaacgca
     4501 tcttccaaat ccccttcata taccgttgat aaataagtta atttaaaatg ttcctgatca
     4561 atcgctgcta agtgtgattc attcaatacc ggccttttca attgaatacg atctaaaacc
     4621 gtttcgtcag gtgctagtaa gttaccttcg ccacctaaat aagaaagttc actcgttacg
     4681 attttttcac gatacgcatc aattggtgga ttcgtaactt gtgcaaacag ctgtttaaag
     4741 taattaaata gtgattctgg tcgctcgttc aacactgcaa ttggcgcatc atatcccatt
     4801 gcaccgatag gatccttctt accttctaca agttcctgaa tatacttatg aatctcttct
     4861 ttcgtgtatg caaactgacg ttgtaattta aataacgtct catctttcca ttgcgaatct
     4921 tgatattgta tattttcaaa atcaaagtca actttatggt tatcaatcca cgctttatat
     4981 ggtaattctc cagcaatcgc accttttaaa tcattatttt caatgacttt attctgttta
     5041 aaatcaacaa gcaataactt tccaggattc aattgacctt taaaagcaac attactttca
     5101 ggtacgtcca caacacccac ttcagatgaa aagacaataa agttatcttt agtaatcgta
     5161 taacgacctg gacgtaatcc atttctatct gtaagcgcgc caagtttgtc accgttacag
     5221 aacgaaatca ttgtaggacc atcccacggt tccattaaat aactataaaa ttcataaaac
     5281 gcacgtacat ttgcatcatt cgcttcatta tataaccaag gttcaggtat gagtaacatc
     5341 gctgcctttt ctggctccat ggctaacgat aagaactcta gcgcattatc tacaatggca
     5401 gagtcactac catcctcatc gacaatttga aacactttat gttgatcctc gccaaataat
     5461 gtttcgatta atttatgttg gcgtgctcgc atccagttta cattaccttt aatcgtgtta
     5521 atctcaccat tatgcattaa catacggtta ggatgtgccc ttttccaact cgggaatgta
     5581 ttcgtactaa atctcgaatg cactaaccct agctttgatt gatataaatc atccgataaa
     5641 tctgtatata gttttttaat ttggtctgat cgtaaccaac ctttatatac aattgttttg
     5701 cgtgataagc tcgtaaaata caattctaaa tcgcactgag tcgaatagaa ctctaattgt
     5761 tttctcgcta aaaacaaacg cttttcaaca tcttcaatgt ccctaatatc aataaacact
     5821 tgttgaatga ctggcatcgt atctgctaca tgtttagcaa tggcatcttt attaactggt
     5881 acattacgat aaccaagaat tgataaccct tcgccttcaa aatatttttt aaaaactact
     5941 tcatgttcag aacctaaaat gcgttctttg gaaaaaaata accccacggc atattcacct
     6001 tcacctggga tatcaaagtc cgttacatgt tgtttgaaaa atgcaaaagg tatttcagtc
     6061 ataatacctg cgccatcacc agtgatgcca tctgcgccga ccccgcccct gtgatctaag
     6121 cgtcgcaaca tttcaagcga tttatcaatg atgtcgtgag accttttatt atccatattc
     6181 gcataaaaac caataccaca cgcatcatgt tcctcacgat agtcatataa gcctttaatt
     6241 aatttctcat tgtgcatgat gcacacccct tttttagaat attctgatat tagttattaa
     6301 tgataattac tataatctaa aaaatttatc ttttcaatat ataaaataga agatattcat
     6361 ctaaaaaata gaacatttat caataatatt gctataatag ttacaaaaca ctcatttttg
     6421 gaggtgccta tatcgctatg gaaattaaac aattaagata ttttatcgag gtcgctaaaa
     6481 gagaacatat ctccgaaaca gcacttgaac taaacattgc tcaatcagcg ataagccgtc
     6541 aaattacatt attagaacaa gaacttaatg tgtctttatt taaaaagcaa ggacgcaata
     6601 ttacgctcac atctgaaggg aaattattat ttaatgaagc tctgcgtatt attgaacatt
     6661 tagatagcac gattgaacaa tttcaaagcc atggattaac taaaaataaa agtatctaca
     6721 ttggctatga tgagagtgac gtatcccaca tgttactacc acttatccaa acgtttcatt
     6781 tacaaaatga tacacatgtc ataccgagct tattagaaca tgacacaatt ataaatagcg
     6841 tattaaatgg gaatatagac attggtttca cggaacttac accagaaata cgtaaacaca
     6901 aacaattaca tatgctacct ttattcgaag agcactatca tttatatgcg ccttcagacg
     6961 atccaattac gatggcgaca cacccaccat taattcagtt tgagcatagt catatttatt
     7021 gcttggcacc atttgccgaa actgtaaaaa aacaattgcg caagattact aaatcagatg
     7081 tatacacaat cagtagccaa ccacttgcgc agtatctcct tcgtcaaaaa gaaggttaca
     7141 tcatttcatc acaaaatata catttaccag aatctaaatc atggatagat ataaaattag
     7201 atcatactga attaaaaaga acgatatgtg ctattacaaa ggagccttat acaaagagtg
     7261 atattggcat attattaaca cttattcagc aattaatgac taaaacgtct acattccatt
     7321 aagtaattcg atgctatcaa tgatgatttt cgttttacac ttagcagtaa tatctttgaa
     7381 aataaaa
//
LOCUS       DAKTIP010000130         7378 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAKTIP010000130 DAKTIP010000000
VERSION     DAKTIP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.130"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            312..686
                     /locus_tag="O0305_001974"
     CDS             312..686
                     /locus_tag="O0305_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCY0275114.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            839..1237
                     /locus_tag="O0305_001975"
     CDS             839..1237
                     /locus_tag="O0305_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0275115.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(1453..2277)
                     /locus_tag="O0305_001976"
     CDS             complement(1453..2277)
                     /locus_tag="O0305_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCY0275116.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(2514..3821)
                     /gene="brnQ"
                     /locus_tag="O0305_001977"
     CDS             complement(2514..3821)
                     /gene="brnQ"
                     /locus_tag="O0305_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0275117.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRLFGSFGNLV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKSFMMLPLADIDLAWLVPFMLFAIIGFIIDVFIRRPKQATT"
     gene            4405..5295
                     /locus_tag="O0305_001978"
     CDS             4405..5295
                     /locus_tag="O0305_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCY0275118.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            5544..6593
                     /locus_tag="O0305_001979"
     CDS             5544..6593
                     /locus_tag="O0305_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCY0275119.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASSVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTVILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            6606..7283
                     /locus_tag="O0305_001980"
     CDS             6606..7283
                     /locus_tag="O0305_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275120.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKQRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
BASE COUNT         2794 a         1059 c         1274 g         2251 t
ORIGIN      
        1 atatttggga attaaataaa tatataggag gcaattttta gagatacata gcaatgtaaa
       61 ggtatcaaat agttatttca taatttatta tgatgacata attttcagta gatagttgta
      121 aattaacact gaaatagatg tttatattaa gaagacttta atatcataat taatcaaaat
      181 cttgcgtcaa acatttaaac gaattttaag ttgataacaa ttggcaaaac attgttcaaa
      241 catcacaatg ataaagcata ttatcagtat tgtagtgtgt ggaaaatgac agccatctaa
      301 ggagaaaaat gatgaaaaga atattggtag tatttttaat gttagcaatt atattggcag
      361 gttgttctaa taaaggtgaa aagtatcaaa aagatattga taaagtgtac aaagaacaga
      421 atcaaatgaa taaaattgcc tcgaaagtac aaaacactat taaaacagac attaaacaag
      481 aagacagtaa tacacatgtt tataaagatg gtaaagtcat tgttattggt attcaattat
      541 ataaagatcg tgaaaaaatg tattatttcg catatgaaat aaaagatggt aaggcagaga
      601 ttaatagaga aatagaccca attaagtata tgaaagacca taaagcagat tatgaagatg
      661 aaaatgtaga agtggaaaaa gattaacata ggctttcttt aaaactttta accagtttca
      721 aaaagtaaat gagctttagc gacaataatt tttaaaataa acacgatata aatttaaaca
      781 tttatatata agaacacaaa cgcaagtttg taaataataa taataaagga gtaagacaat
      841 ggaaaaatcg atcaaaataa tgacaataat aggaattgtt gttcagggtt tagcaacggt
      901 atttagttta ctattgatgg ttttagcagc atcaggtgta atgactacag atgtgtcaac
      961 aacagttaat ggtgaggttg acccagttga tgcagaaaca gcagcagcaa ttttcactgt
     1021 attattctta tccctattca tatttggaat catttcaatt attttaggtg caatcggtat
     1081 gtttaaagca tctaaaaaca aaaaaatgag tggtatattg ttgattattg gagctgtaat
     1141 aagtggtaac ataattacat ttgctttatg gttaatcagt ggtattaaac tacttactaa
     1201 taacaagcct aaagatgaaa taagcgactt atcataaaca tcgtatattg aaatttcaag
     1261 atttcttaag taattaaata aagtgctctc ttagaagtag attttcagtc aataactgct
     1321 tttaagagag cattttgtat ttgataatgc tcatagcatc ggataaaaat cattgacgaa
     1381 ctttaaaaaa atacgttaga caatgcactt ctaaatatta aaggcatcga ctaacgcatt
     1441 gttcaaatat agttaattat ttttataaaa ttgatgatga tcattcaagt aagcatagaa
     1501 taaacctata atgagtccgc ctccaatata gttaccgata aaagccgcag cgatattcga
     1561 aatagctggt atgaagtgca atgtatcaac ttgataaatt aaaccaccca taaataagca
     1621 actgttgtaa acgacgtgtt cataacccat aaaggcgaat atggtaacac cgaacatcat
     1681 gacaaacatt tttgcgagta catcgtcaat ttgcatggca ataactaatg aaatattgat
     1741 aaagaaattg gcgaagatcg ctttcattaa tatacttaca aaaccagtag acaacgtttt
     1801 atgctctata actgctgata actgatttaa catatctggc gtcattacat ttgaaaaacg
     1861 catgaaacta aataaaatag cagcacctaa aatatttcct gcaaagcata ataaaaatat
     1921 tttcaatact ctagttggtt taattacttt ataatacagg cctacagtaa agtacatgaa
     1981 gttactggtt agtagttcgg agtttgtaaa taaaatgagt actaacgcaa agctgaatgt
     2041 aatggcactg gccatattca caatgcctgg cggtaaatct ggttcgtgtg ttgctttaac
     2101 tgataatacg aagaccgtaa taatcccgat aataaatcct gccatcatcg cgcgtaataa
     2161 ataacgtttt aaataaacgc tttgtaatat atcttttgtt cttatcgttt cgactacgtt
     2221 atttacccag tcgtccccat aaaaaatctt atcccattta atatgtttct ccttcacaat
     2281 gacatcctca cttttcattt gttaatcaga gtatactatg aattgcctta tttgtgaata
     2341 atttcacaaa attaatttta aaaactaata tttttgaata acagctagga ttttttaaca
     2401 aatcatttat tttaaaataa tcaatagaca gacggtatca ctgaaaagtc atgaatttca
     2461 tataaaaaag cctagtaacg atgggtcaat cattactagg cagtgagcat ttattaagtt
     2521 gtcgcttgtt tcggacggcg tataaataca tcgattatga aaccgataat agcaaagagc
     2581 atgaatggta caagccaagc taaatcgata tctgctaaag gtaacatcat aaacgatttc
     2641 aaaataacac cgtgtaataa gttgaaacta tttagtattt gtaaaattga aataatcaat
     2701 gtaataacag ttgcgagtcg ataggcccaa ctgaatctga atgtgctaaa catgttagca
     2761 aatgatatga gtacaagtgc aatcgacacg ggatatatta aagtcaataa tgggacagca
     2821 atttttaaaa tcatttctaa accaagtgtt gtaaataaga accctatgat agagaaaata
     2881 agtgcgaata ttttataaga aaacttaggt acgtgtttct tagtaaatgt ggcgcaagca
     2941 ttgacgagtc ctatacatgt tgttaggcat gcaaggataa ccgtcattcc aaatacgagg
     3001 ttaccgaacg aaccaaataa tcgtaatgag ttgtacgtca atatatctgt accatcttta
     3061 aagtttcctg gagctgttga tgccccaacg tatgcaagtg caaagtaaat cattccaagt
     3121 aatatggctg caataagacc tgaaaagcag acatatttta aaattttcat gcgatctgtg
     3181 aggcctttaa acttatagcc attgacaatg actacggaaa aagctaacgc agcaacaaga
     3241 tccattgtaa aatagccttc caaacttcct gaaatgaaag gatgtgttat atatttatcc
     3301 ttaggtgcac ttagtgcaga ttcagggttg aaaatgacag caatacttaa tagagcgacc
     3361 attaatagta ataacggtgt taataattta cctaaattat caacgatttt cgatggattt
     3421 aaactaatcc agtaaacgat ggcaaaaaag attgctgcga atataattaa agtccattgg
     3481 ttatgcacag gtaaaatgtg tcttgtacca atttcgtacg cgacatttgc agcacgtgga
     3541 ataccgtaaa atgctccgat agacatgtaa atcacgacag caaaaataaa cccgaaccat
     3601 ggatgtatac gattgcctac actttcaaca ccttcatcat aaaatgcaac aacaataaca
     3661 gtaataaagg ggagtaatat gcctgtaagg gcaaagccta gcataccaat ccacatattt
     3721 tgacccgctg tatggccaag catgggcggg aatattaaat ttccggctcc aaaaaatagt
     3781 gaaaataaca tgaggcccga aataataact tgttttttca atgtaatttc cttcctaaaa
     3841 ctagatattc ataataattt aaaaaatctg aaaaataaaa acgttcttac tttatcttta
     3901 aatgacaata ctatgattct attattttat aaaaaagatt gcaataataa cgtcttgcta
     3961 ttattggata atgacaatta aagtgtcgtt tttatgaatt aaaataggtg aaagtaagtt
     4021 ggaaatacgt tggagcgttg tgatttttat gagaaattgt atgagtgagg actgctattt
     4081 ataaaaggta aactatggat gagtttatgg ttgttccaat aggtaaataa gagatagcac
     4141 actgtacaca taatgatacg tggatgagtg aatatgtctg ataggaaggc ctagtcactc
     4201 aaatgaattt aagtttatta cgtgataaat cacaaatctc tctcatgtga taggtctccc
     4261 attaaatcat gcttatatga aatgttcaca tatttgttag cttttcaaga aataatatta
     4321 aatatctttt catagtatag caatattaaa taaatgcatt tataatttta actgtatttt
     4381 aaatattaat catgaggtga taagatgaat aaaatttcaa agtatattgc aatagcatca
     4441 ttatcggtag cggttacagt ttcagcacca caaacgacaa attctacagc gtttgccaaa
     4501 agttctgctg aagttcaaca aacgcaacaa gcttctatac cagcatcaca aaaggcgaat
     4561 cttggtaatc aaaatattat ggcagtggct tggtatcaaa attcagctga agcaaaagca
     4621 ttatatttac aaggttataa cagtgcaaag acacagttag ataaagagat taaaaagaat
     4681 aaaggtaaac ataagttagc tattgctttg gatttagatg aaacagtttt agataattct
     4741 ccatatcaag gctatgcatc aatacataat aaacctttcc cagaaggttg gcatgaatgg
     4801 gtacaagctg ctaaagctaa acctgtctat ggcgcaaaag aattcttgaa atatgctgac
     4861 aaaaaaggtg tcgatatcta ctatatttct gatagagata aagaaaaaga tttaaaggca
     4921 acacaaaaga acttaaaaca acaaggtatc cctcaagcta agaagagtca tattttacta
     4981 aaaggtaaag atgataagag taaagaatca cgcagacaaa tggttcaaaa ggatcataaa
     5041 cttgtcatgc tatttggaga taatttatta gactttacag atccaaaaga agctacagct
     5101 gaatctcgtg aagcattaat tgaaaaacat aaagacgatt tcggtaagaa atatatcatt
     5161 ttccctaacc caatgtatgg tagttgggaa gctacgattt acaacaataa ctataaagca
     5221 agtgacaaag caaaagataa attacgtaaa aatgctatta agcaattcga tcctaaaaca
     5281 ggcgaagtta aataatatat gaattggacg tctacatgta cttaaaggta tatgtaggcg
     5341 tttttaaatt gatccttaat tttaaatgta ccgtacaaaa ataatagaca attaattgca
     5401 ttttccataa atttgctttt aatataaaaa gttcgggtat aatgaaggat aagtgtatgt
     5461 ataacattac agagcgcaac acgacgtacg tgcatctaag aatataaaca atgtttaaat
     5521 catagtagta gggagagaaa tgcatgtttt tagcttggaa tgaaatacgg cgcaacaaat
     5581 tgaagtttgg actaattatt ggtgtgttaa cgatgattag ttacttgcta tttttattat
     5641 ctggattggc gaatggtctt atcaatatga ataaagaagg cattgataag tggcaagcag
     5701 atgccattgt tctaaataaa gatgccaatc aaactgtgca acaatctgtt tttaacaaga
     5761 aagatattga aaataaatac aagaagcaag ctactttgaa gcaaacaggg gaaattgtgt
     5821 ctaatggcca tcaaaaagac aatgttttag tgttcggtgt tgaaaagtca tcatttttag
     5881 ttccgagttt aatagaaggg cataaagcga ctaaagataa tgaagtgtta gctgatgaaa
     5941 cgcttaaaaa taaaggattt aaaattggtg acacattatc actatctcaa tcagatgaaa
     6001 aattgcatat cgtaggtttt acagaaagtg caaaatataa tgcgtcatca gtcattttca
     6061 cgaatgacgc taccattgcc aagatcaatc ctagattgac tggagataaa attaatgcag
     6121 ttgttgtacg tgatacaaat tggaaagaca aaaaattaaa ccaagagctt gaagcggtaa
     6181 gtattaatga ctttattgaa aatttaccag gttataaacc acagaactta acattaaact
     6241 ttatgatttc attcttattt gtcatttcag ctacagttat aggcattttc ctatatgtca
     6301 tgacattaca aaagacgagt ttatttggca tattaaaagc tcaaggattt acgaatggct
     6361 atttggcgaa tgtggtaatt tcgcagacgg tcatattagc actatttggt acggcatttg
     6421 gcttactgtt aacaggcgtt acaggtgcat ttttacctga tgcagtacct gtcaaattcg
     6481 atgtactaac attgctcgta tttgcaattg tgttaatgat tgtctctgta ttaggaagtt
     6541 tattctccat tttaacaatt agaaaaatag atccgttaaa ggcgattggg taggaggtgt
     6601 agcaaatgtt gaaatttgaa aatgtaacaa agtcatttaa agatgggaat cgtaacattg
     6661 aagcggttaa agatacaaat tttgagataa ataaaggtga tattatagca ttggttggac
     6721 cttctggctc tggtaaaagt acatttctaa ctatggcagg tgctttacaa acaccgacat
     6781 ctgggcacat tttaatcaat aaccaagata ttacgacaat gaagcaaaaa gcattggcaa
     6841 aagttagaat gtctgaaata ggttttattt tacaagctac aaaccttgta ccatttttaa
     6901 cggtaaagca acaatttaca ttattgaaaa agaaaaataa gaatgttatg tctaatgaag
     6961 actatcagca acttatgtca caattaggtc taacttcatt gcttaataag ttaccttcag
     7021 aaatttcagg tggtcagaaa caacgtgtgg cgatagccaa agcgttatat acgaatccgt
     7081 cgattatttt agcggatgaa cctaccgcgg cgttagatac tgaaaatgcg attgaagtca
     7141 ttaaaattct acgtgatcaa gccaaacaaa gaaagaaagc atgtattatt gttacacatg
     7201 atgaacgact taaagcatat tgtgatcgtt catatcatat gaaagatggc gtccttaatc
     7261 ttgaaaatga aacagtagaa tagttttatt aagccggtac atcatgtgcc ggtattttta
     7321 tgtttatgta ttatttgaat aaactttcac attcaattaa taataattat tatcgaaa
//
LOCUS       DAKTIP010000131         7330 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAKTIP010000131 DAKTIP010000000
VERSION     DAKTIP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.131"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            58..747
                     /locus_tag="O0305_001981"
     CDS             58..747
                     /locus_tag="O0305_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0275121.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(1052..1465)
                     /locus_tag="O0305_001982"
     CDS             complement(1052..1465)
                     /locus_tag="O0305_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0275122.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            1670..2968
                     /locus_tag="O0305_001983"
     CDS             1670..2968
                     /locus_tag="O0305_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCY0275123.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            3229..4170
                     /gene="pde2"
                     /locus_tag="O0305_001984"
     CDS             3229..4170
                     /gene="pde2"
                     /locus_tag="O0305_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCY0275124.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGNEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            4191..>7330
                     /locus_tag="O0305_001985"
     CDS             4191..>7330
                     /locus_tag="O0305_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCY0275125.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEINQLDINQEYLTQVDV
                     IAQKCDAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNYKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVVLSFY
                     DETIKQMTTLGYINQK"
BASE COUNT         2572 a         1023 c         1367 g         2368 t
ORIGIN      
        1 taattaagtg gcaaaagggt actaaattta tatagtaatt gataggagga tgctcaaatg
       61 aagttatcat ttcatggtca atcaacgatt tatttagaag ggaacaacaa aaaggttatc
      121 gtagatccgt ttatttcaaa taaccctaag tgtgatttga atattgaaac agtgcaggta
      181 gactatatcg ttttaacgca cggtcatttt gatcattttg gtgatgttgt ggaactagca
      241 aagaaaacag gagcaacagt aattggaagt gcagaaatgg ctgactatct ttcttcatat
      301 cacggtgttg aaaatgttca tggtatgaat attggtggta aagcaaactt tgatttcgga
      361 agcgttaaat ttgttcaagc atttcatagt tcaagtttta cacatgaaaa tggtattcca
      421 gtttatttag gtatgccaat gggtatcgtg tttgaagttg aaggaaaaac aatttatcat
      481 actggtgata caggtctgtt tagtgacatg tcacttattg cgaaacgtca tccagttgat
      541 gtatgttttg taccaattgg agataatttt acgatgggta ttgatgatgc aagttatgct
      601 attaatgaat ttattaaacc aaaaatttct gtaccgattc attatgatac attcccatta
      661 atcgaacaag atccacaaca atttaaagat gctgttaatg taggggacgt gcaaatttta
      721 aaaccaggtg aatctgttca gttttaaata gaatatagat tttacactaa aaaacacctc
      781 gctaatctat gtggaagtta tcgtctgcat atttttagtg ggtgttttta tattctactg
      841 attcagttac gacgtggacg ggggacatca gtggttcttt atcatttctg acctactctc
      901 gtaattttag ttttatacaa ttagacattg attataaatt tactacgata taaaaaatcc
      961 agaaccacag tcataatcgt ttcttgtatt tactcagtcg tttgagactg catcatgatt
     1021 gaaagattat ttgtgattct ggataaaaat tttatttaac gattaataca gggatagtcg
     1081 cacgttttgc tactttgtgg ctgacacttc caagtacaaa tttctttttg tcttctgctt
     1141 tacggttact taaaacaaca atttcgtatt taccgctatt agcatgtttt aatagttctt
     1201 cttttgcatt accacgaaca attatttggt cataatcaat accataatct tctaaagctt
     1261 tgcgtgtttt ttccaatcgc ttgcttcgtt cttcagtaag tttgtttaaa tgaacacctg
     1321 ctttaattga tgcttgagca tcttgttcgc tgattgcgtt taaaactgtt acgactgtac
     1381 cttcgccagc taatttagac acttctttta gtgctttttc attttttaac tgagtgtcta
     1441 caccaagtaa tatattttta tacattagta catccctcct cacttacatt ctaataaata
     1501 ataatggatt tttccattta aaattaaaga ttttgtgatc tttgtgtttg ttgtatattt
     1561 tcaaaaaata aaataggtga aacgatcatt atttcatttc acttcaatgc tttttatcca
     1621 aaataaagtt ataatgtagt taatactgtt cgaaaagaag aggtaaagaa tgacaaaaca
     1681 tgaacaaatt ttagattata ttgaatcgtt atctataggc tctaaaattt cggtgcgtaa
     1741 aatcgctaaa tttttaaacg tatctgaagg gacagcatat cgtgcaatta aagatgctga
     1801 taaaatgggc atggtcgcaa cgatagatcg tgttggtacg gttcgaatcg aaaaacgtaa
     1861 tcgtaatgaa attgaacatt taacttttaa tgaaattgtc aatattattg atggccaagt
     1921 gttaggtggc aacaaaggta tcactaagat ggtatctaaa tttgcaattg gagcaatgga
     1981 actcaaagat attttaaagt atattggtcc aaagacatta ttgattgtcg gaaatcgtga
     2041 agatgtgcag attgaagctt taaaaagagg tacggctata ttaattactg gtggatttaa
     2101 gccatctaat aaagttatta attttgcgaa tgaacatgat ttacctgttc tatcatcaag
     2161 ttatgataca ttcctagttg caaatattat caacaaagca ctttttaatc agaaaatacg
     2221 aaaagatatc ttaatagttc aagatataat gacgccatta gatgatttat ctgtgctgtt
     2281 tgatacgatg aaaatagcag attataagcg tatggcgaat cgaactggcc atacaagatt
     2341 tccggtagtc aatgagtcgt ataaattagt aggtattgta acaagtcgag agatgattaa
     2401 tacaaaggac gacgacgaaa tagataaagt gatgacgcga aatcctattt atgtaaatgc
     2461 tatgagtaca gtagcaagtt gtgcgcatat gatgatttgg gaaggtattg aattgattcc
     2521 tgtcgtttcg agtaataaaa agacagtggg tgtgattaat agacaagatg ttttaaagtc
     2581 tatgcaactt ttaggaagac agcctcagat gggtgaaaca attaacgatc aaatcgccaa
     2641 atatataaca atgaatcaag atggaattac agtagaagtt tcaccacttt taatcaatca
     2701 ttacggaaca gtaagtaaag cggcatttgt aagtattata gaagaaacaa tacaatatga
     2761 aatgcgtaaa tttaaaaaag gtaatgtcat gatagagaac ttaaatatcg tgtatattaa
     2821 aacagtacct attgaatcac atataacagt gcgctttggc atactagacg tcggaagaaa
     2881 cttcgccaaa atagaagtga acatgcatag tcaaaacgac aaagtagcga gcgctctcgt
     2941 gatatgccaa atgtttgacg aagtatagga acttagcaga gaaaataggc ataagataga
     3001 tttcgcacaa acgttggggc cccaccccaa cttgcattgt ttgtagaaat taggggtcca
     3061 atttctctgt gttggggccc ctgaactaaa tagtatgaaa atatatatat ctaaataatt
     3121 aaaacgggca aatagtttaa tatggtagta acaattttta taatttagat attttgatat
     3181 agactaatgc ttaaattaag caacttttct aaaaaaggga gtatgaaaat gattagtact
     3241 ttgaatgaaa ttatgaaatg tatcgaagac aacgatacaa ttattataca cagacatgtt
     3301 agaccagatc ctgatgcata tggttcacaa cttggtttaa aatactatat tcagcagaaa
     3361 tttccgcaaa agcaagtatt tgcagtgggt gaagcggaat catcattaag ttttattgga
     3421 gagttagata acattgatga taaaacatat caagatgcgc ttgtaattgt atgtgatact
     3481 gccaatgctc cacgaattga cgatgaacgt tatagtacag gtcgtaaact tattaaaata
     3541 gatcatcatc ctgcagttga tcagtatggt gatattaatt tagttaatac gaacgcgtca
     3601 tctacaagtg aaatcattta cgatttaatc tcacatttta atgatgaagc aattgttaat
     3661 aaagatatcg cgagcgtttt atatcttggt atcgtcggtg atactgggcg attccttttt
     3721 aacaatacct cagaacatac tatggaaatt gctggaaagt taattgggca tgatattgat
     3781 cataatgcgc ttttaaataa aatgatggag aaggacccaa aaatgttgcc gtttcaaggt
     3841 tatgttttac aacatttcga acttatggat gatggattct gccaagttaa aataactgaa
     3901 gatgtattgg agcaattcgg tattcagcca aatgaagcat ctcagtttgt taatacaatt
     3961 gctgacatca aaggcttgaa aatatgggta tttgcagtcg atgaaggtaa tgaaatcaga
     4021 tgtcgattac gttctaaagg gcaattgatt attaatgata ttgcgcaaga ttttggtggc
     4081 ggtggtcatc cgaatgcgtc aggagtttca gtggacagct gggatgaatt tgagcaactt
     4141 gctacagctt tacgcacaaa acttaactaa tagaaagggg ccattcaatc atggtggcat
     4201 atttaaatat tcatacggct tatgatttgt taaattcaag cttaaaaata gaagatgccg
     4261 taagacttgc tgtgtctgaa aatgttgatg cacttgccat aactgacacc aatgtattgt
     4321 atggttttcc taaattttat gatgcatgta tagcaaataa cattaaaccg atttttggta
     4381 tgacaatata tgtgacaaat ggattaaata cagtcgaaac agttgttcta gctaaaaata
     4441 atgatggatt aaaagatttg tatcaactat catcggaaat aaaaatgaat gcattagaac
     4501 atgtgtcgtt tgaattatta aaacgatttt ctaacaatat gattatcatt tttaaaaaag
     4561 tcggtgatca acatcgtgat attgtacaag tgtttgaaac ccataatgac acatatatgg
     4621 accaccttag tatttcgatt caaggtagaa aacatgtttg gattcaaaat gtttgttacc
     4681 aaacacgtca agatgccgat acgatttctg cattagcagc tattagagac aatacaaaat
     4741 tagacttaat tcatgatcaa gaagattttg gtgcacattt tttaactgaa aaggaaatta
     4801 atcaattaga tattaaccaa gaatatttaa cgcaggttga tgttatagct caaaagtgtg
     4861 atgcagaatt aaaatatcat caatctctac ttcctcaata tgagacacct aatgatgaat
     4921 cagctaaaaa atatttgtgg cgtgtcttag ttacacaatt gaaaaaatta gaacttaatt
     4981 atgacgtcta tttagagcga ttgaaatatg agtataaagt tattactaat atgggttttg
     5041 aagattattt cttaatagta agtgatttaa tccattatgc gaaaacgaat gatgtgatgg
     5101 taggtcctgg tcgtggttct tcagctggct cactggtcag ttatttattg ggaattacaa
     5161 cgattgatcc tattaaattc aatctattat ttgaacgttt tttaaaccca gaacgtgtaa
     5221 caatgcctga tattgatatt gactttgaag atacacgccg agaaagggtc attcagtacg
     5281 tccaagaaaa atatggcgag ctacatgtat ctggaattgt gactttcggt catctgcttg
     5341 caagagcagt tgctagagat gttggaagaa ttatggggtt tgatgaagtt acattaaatg
     5401 aaatttcaag tttaatccca cataaattag gaattacact tgatgaagca tatcaaattg
     5461 acgattttaa aaagtttgta catcgaaacc atcgacatga acgctggttc agtatttgta
     5521 aaaagttaga aggtttacca agacatacat ctacacatgc ggcaggaatt attattaatg
     5581 accatccatt atatgaatat gcccctttaa cgaaagggga tacaggatta ttaacgcaat
     5641 ggacaatgac tgaagccgaa cgtattgggt tattaaaaat agattttcta gggttgagaa
     5701 acttatcgat tattcatcaa atcttaacac aagtcaaaaa agatttaggt attaatattg
     5761 atatcgaaaa gattccgttt gatgatcaaa aagtgtttga attgttgtcg caaggagata
     5821 cgactggcat attccaatta gagtctgacg gtgtaagaag tgtattaaaa aaattaaagc
     5881 cggaacactt tgaagatatt gttgctgtaa cttctttgta tagaccaggt ccaatggaag
     5941 aaattccaac ttacattaca agaagacatg atccaagcaa agttcaatat ttacatccgc
     6001 atttagaacc tatattaaaa aatacttacg gtgttattat ttatcaagag caaattatgc
     6061 aaatagcgag cacatttgca aacttcagtt atggtgaagc ggatatttta agaagagcaa
     6121 tgagtaaaaa aaatagagct gttcttgaaa gtgagcgtca acattttata gaaggtgcaa
     6181 agcaaaatgg ttatcacgaa gacattagta agcaaatatt tgatttgatt ctgaaatttg
     6241 ctgattatgg ttttcctaga gcacatgctg tcagctattc taaaattgca tacattatga
     6301 gctttttaaa agtccattat ccaaattatt tttacgcaaa tattttaagt aatgttattg
     6361 gaagtgagaa gaaaactgct caaatgatag aagaagcaaa aaaacaaggt atcactatat
     6421 tgccaccgaa cattaacgaa agtcattggt tttataaacc ttcccaagaa ggcatttatt
     6481 tatcaattgg tacaattaaa ggtgttggtt atcaaagtgt gaaagtgatt gttgatgaac
     6541 gttatcagaa cggcaaattt aaagatttct ttgattttgc tagacgtata ccgaagagag
     6601 tcaaaacgag aaagttactt gaagcactga ttttagtggg agcgtttgat gcttttggta
     6661 aaacacgttc aacgttgttg caagctattg atcaagtgtt ggatggcgat ttaaacattg
     6721 aacaagatgg ttttttattt gatattttaa cgccaaaaca gatgtatgaa gataaagaag
     6781 aattgcctga tgcacttatt agtcagtacg aaaaagaata tttaggattt tatgtttcgc
     6841 aacacccagt agataaaaag tttgttgcca aacaatattt aacgatattt aaattgagta
     6901 acgcgcagaa ttataaacct atattagtac agtttgataa agttaaacaa attcgaacta
     6961 aaaatggtca aaatatggca ttcgtcacat taaatgatgg cattgaaact ttagatggtg
     7021 tgattttccc taatcagttt aaaaagtacg aagagttgtt atcacataat gacttgttta
     7081 tagttagcgg gaaatttgac catagaaagc aacaacgtca actaattata aatgagattc
     7141 agacattagc cacttttgaa gaacaaaaat tagcatttgc caaacaaatt ataattagaa
     7201 ataaatcaca aatagatatg tttgaagaga tgattaaagc tacgaaagag aatgctaatg
     7261 atgttgtgtt atccttttat gatgaaacga ttaaacaaat gactacttta ggctatatta
     7321 atcaaaaaga
//
LOCUS       DAKTIP010000132         7322 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAKTIP010000132 DAKTIP010000000
VERSION     DAKTIP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.132"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            133..1137
                     /gene="ilvC"
                     /locus_tag="O0305_001986"
     CDS             133..1137
                     /gene="ilvC"
                     /locus_tag="O0305_001986"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCY0275126.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            1167..2696
                     /locus_tag="O0305_001987"
     CDS             1167..2696
                     /locus_tag="O0305_001987"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0275127.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            2699..3745
                     /gene="leuB"
                     /locus_tag="O0305_001988"
     CDS             2699..3745
                     /gene="leuB"
                     /locus_tag="O0305_001988"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCY0275128.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            3759..5129
                     /gene="leuC"
                     /locus_tag="O0305_001989"
     CDS             3759..5129
                     /gene="leuC"
                     /locus_tag="O0305_001989"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCY0275129.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            5130..5702
                     /gene="leuD"
                     /locus_tag="O0305_001990"
     CDS             5130..5702
                     /gene="leuD"
                     /locus_tag="O0305_001990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCY0275130.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            5717..6985
                     /gene="ilvA"
                     /locus_tag="O0305_001991"
     CDS             5717..6985
                     /gene="ilvA"
                     /locus_tag="O0305_001991"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCY0275131.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
     gene            complement(7195..7309)
                     /gene="rrf"
                     /locus_tag="O0305_001992"
     rRNA            complement(7195..7309)
                     /gene="rrf"
                     /locus_tag="O0305_001992"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2486 a         1180 c         1417 g         2239 t
ORIGIN      
        1 aagacaaagg caatgatgcg ctaattagtt atagatatat cataggctgc tagttaacat
       61 ctgccactat tacaaagtta tatttcagaa ttttcgaaac acaaaatatt taattatttg
      121 gaggaattta ttatgacaac agtttattat gatcaagatg taaaaacgga cgctttacaa
      181 ggcaaaaaaa ttgcagtagt aggttatgga tcacaaggtc acgcgcatgc acaaaactta
      241 aaagacaatg gatatgatgt agtcatcggc attcgcccag gtcgttcttt tgacaaagct
      301 aaagaagatg gatttgatgt gttccctgtt gcagaagcag ttaagcaagc tgatgtaatt
      361 atggtgctat tacctgatga aattcaaggt gatgtataca aaaacgaaat tgaaccaaat
      421 ttagaaaaac ataatgcgct tgcatttgct catggcttta acattcattt tggtgttatt
      481 caaccaccag ctgatgttga tgtattttta gtagctccta aaggaccggg tcatttagtt
      541 agacgtacat ttgttgaagg ttctgctgta ccatcactat ttggtattca acaaggcgct
      601 tcaggtcaag cacgtaatat tgctttaagt tatgcaaaag gtattggtgc aactcgtgca
      661 ggtgttattg aaacaacatt taaagaagaa actgagacag atttatttgg tgaacaagca
      721 gtactttgcg gtggtgtatc gaaattaatt caaagtggct ttgaaacatt agtagaagcg
      781 ggttatcaac cagaattagc ttattttgaa gtattacatg aaatgaaatt aatcgttgat
      841 ttgatgtatg aaggcggtat ggaaaatgta cgttactcaa tttcaaatac tgctgaattt
      901 ggtgactatg tttcaggacc acgtgttatc acaccagatg ttaaagaaaa tatgaaagct
      961 gtattaactg atatccaaaa tggtaacttc agtaatcgct ttatcgaaga caataaaaat
     1021 ggattcaaag aattttataa attacgcgaa gaacaacatg gtcatcaaat tgaaaaagtt
     1081 ggtcgtgaat tacgcgaaat gatgcctttt attaaatcta aaagcattga aaaataagat
     1141 agacctacaa tgaggagttg ttaaatatga gtagtcatat tcaaattttt gatacgacac
     1201 taagagacgg tgaacaaaca ccaggagtga attttacttt tgatgaacgc ttgcgtattg
     1261 cattgcaatt agaaaaatgg ggtgtagatg ttattgaagc tggatttcct gcttcaagta
     1321 caggtagctt taaatctgtt caagcaattg cacaaacatt aacaacaacg gctgtatgtg
     1381 gtttagctag atgtaaaaaa tctgacatcg atgctgtata tgaagcaaca aaagatgcag
     1441 cgaagccggt cgtgcatgtt tttatagcaa catcacctat tcatcttgaa cataaactta
     1501 aaatgtctca agaagacgtt ttagcatcta ttaaagaaca tgtcacatac gcgaaacaat
     1561 tatttgacgt tgttcaattt tcacctgaag atgcaacgcg tactgaatta ccattcttag
     1621 tgaaatgtgt acaaactgcc gttgacgctg gagctacagt tattaatatt cctgatacag
     1681 tcggctacag ttaccatgat gaatatgcac atattttcaa aaccttaaca gaatctgtaa
     1741 catcttcaaa tgaaattatt tatagtgctc attgccatga cgatttagga atggctgttt
     1801 caaatagttt agctgcaatt gaaggcggtg cgagacgaat tgaaggcact gtaaatggta
     1861 ttggtgaacg agcaggtaat gcagcacttg aagaagtcgc gcttgcacta tacgttcgaa
     1921 atgatcatta tggtgctcaa actgctctta atctcgaaga aactaaaaaa acatcggatt
     1981 taatttcaag atatgcaggt attcgagtgc ctagaaataa agcaattgtt ggccaaaatg
     2041 catttagtca tgaatcaggt attcaccaag atggcgtatt aaaacatcgt gaaacatatg
     2101 aaattatgac acctcaactt gttggtgtaa gcacgactga acttccatta ggaaaattat
     2161 ctggtaaaca cgccttctca gagaagttaa aagcattagg ttatgacatt gataaagaag
     2221 cgcaaataga tttatttaaa caattcaagg ccattgcgga caaaaagaaa tctgtttcag
     2281 atagagatat tcatgcgatt attcaaggtt ctgagcatga gcatcaagca ctttataaat
     2341 tggaaacact gcaactacaa tatgtctcta gcggccttca aagtgctgtt gttgttgtta
     2401 aagataaaga gggtcatatt taccaggatt caagtattgg tactggttca atcgtagcaa
     2461 tttacaatgc agttgatcgt attttccaga aagaaacaga attaattgat tatcgtatta
     2521 attctgtcac tgaaggtact gatgcccaag cagaagtaca tgtaaattta ttgattgaag
     2581 gtaagactgt caatggcttt ggtattgatc atgatatttt acaagcctct tgtaaagcat
     2641 acgtagaagc acatgctaaa tttgcagctg aaaatgttga gaaggtaggt aattaattat
     2701 gacttataac attgttgccc tacctggtga tggaatcggt ccagaaattt tgaacggatc
     2761 tctatcattg cttgaaatta taagtaataa atataacttt aattatcaaa tagagcacca
     2821 cgaatttggt ggtgcctcta ttgatacatt cggcgagcct ttaactgaga aaaccttaaa
     2881 tgcgtgtaaa agagcagatg ctattttact gggtgcaatc ggtggaccta aatggacaga
     2941 tcctaacaat cgaccagaac aaggattatt aaaattgcgt aaatccttaa atttatttgt
     3001 aaatatacgc cccactaccg ttgtcaaagg cgctagttct ttatcacctt taaaggaaga
     3061 acgcgttgaa ggcacagatt tagttatagt ccgtgaattg acaagtggta tttattttgg
     3121 agaacctaga cattttaata atcacgaggc cttagattct cttacttata caagagaaga
     3181 aatagaacgc attgttcacg tagcatttaa attggccgct tcaagacgag gaaaactaac
     3241 atcagttgat aaagaaaatg tattagcttc tagtaaattg tggcgcaaag tcgtaaatga
     3301 agtaagtcaa ttatatccag aagtaacagt aaatcactta cttgttgatg cttgtagtat
     3361 gcatttaatc acaaatccaa aacaatttga cgtcatcgta tgtgaaaact tatttggcga
     3421 tattttaagt gatgaagctt cagtgattcc tggttcactt ggtttatcac cttctgctag
     3481 ttttagtaac gatggtccaa gattgtatga gcctattcat ggatcagcac cagatattgc
     3541 aggtaaaaac gttgccaatc catttggaat gattctatct ttagcgatgt gtttacgtga
     3601 aagcttaaat caaccagatg ctgcagatga attagaacaa catatttata gcatgattga
     3661 acatgggcaa acgacagcag atttaggcgg caaattgaat actactgata ttttcgaaat
     3721 tctatctcaa aaattgaatc actaaggggg agatgtaaat gggtcaaaca ttatttgaca
     3781 aggtgtggaa cagacatgtg ttatacggga aattgggcga accgcaacta ttatacattg
     3841 atttacacct tatacatgaa gttacttctc ctcaagcatt tgaaggactt aggcttcaaa
     3901 acagaaaatt aagacgccca gatttaacat ttgcaacact cgatcacaat gttcctacta
     3961 ttgatatatt caatattaaa gatgaaattg caaacaaaca aatcacaaca ttacaaaaaa
     4021 acgccataga ttttggggtg catatttttg atatgggttc tgatgaacaa ggtattgttc
     4081 acatggtagg acctgagaca ggacttacac agcctggcaa gacaatcgtt tgtggtgact
     4141 ctcacacagc aacacatgga gcatttggtg ctattgcatt tggaattgga acaagtgaag
     4201 ttgaacatgt tttcgcaact caaacgctat ggcaaacaaa acccaaaaac ttaaaaatcg
     4261 atattaatgg taccttacca acaggcgtct atgctaagga cattattctg catttaatta
     4321 aaacgtatgg tgttgacttt ggtacaggct atgctttgga atttactggc gaaacaatta
     4381 aaaacctttc aatggatggt cgaatgacta tttgtaacat ggctatcgaa ggtggtgcca
     4441 aatacggcat aatccaacct gatgatataa catttgaata tgttaaaggg agaccatttg
     4501 ccgataactt cgctaaatca gttgataagt ggcgtgagct atattctgat gacgacgcga
     4561 tatttgatcg tgtaattgaa cttgatgttt caacattaga accacaagtg acatggggaa
     4621 ctaatcctga aatgggtgtt aatttcagtg aaccattccc tgaaatcaat gatatcaacg
     4681 atcaacgtgc gtatgattat atggggttag aaccaggtca aaaagctgaa gacatcgact
     4741 tagggtatgt ttttctcggt tcatgtacaa atgctagact atcagatttg attgaagcta
     4801 gtcatattgt taaaggaaat aaagttcatc caaatattac agctattgtc gtaccaggtt
     4861 ctcgtacagt aaaaaaagaa gcagaaaaat taggtctaga tactatcttt aaaaatgcag
     4921 gatttgaatg gcgtgaacca ggatgttcaa tgtgtttagg catgaatcct gaccaagtac
     4981 ctgagggcgt acattgtgca tctacaagta atcgaaactt tgaaggacga caaggcaaag
     5041 gtgcaagaac acatttagta tcccctgcta tggcagcagc agcagctatt catggtaaat
     5101 ttgtggacgt aagaaaggtg gttgtttaaa tggcagcaat caaacctatt acaacatata
     5161 aaggtaaaat agtccctctc ttcaacgaca atatcgatac agaccaaatc attcctaagg
     5221 tacacttaaa gcgtatttca aaaagtggct ttggtccatt tgcttttgat gaatggcggt
     5281 acttacctga tggttcagat aatcctgatt tcaatcctaa caaaccacaa tataaagggg
     5341 cttctatttt aattactgga gataattttg gatgtggttc aagtcgtgaa catgctgctt
     5401 gggctcttaa ggactatggt tttcatatta ttattgcagg aagtttcagt gacatatttt
     5461 atatgaattg cactaaaaat gcgatgttgc ctatcgtttt agaaaaaagt gcccgtgaac
     5521 atcttgcaca atatgttgaa attgaggtcg atttaccaaa tcaaactgtg tcatcaccag
     5581 acaagcgttt ccattttgaa attgatgaaa cttggaagaa taaacttgta aatggcttag
     5641 atgacattgc aatcacccta caatatgaat cattaataga aaaatatgaa aaatcacttt
     5701 aagggagttg aatattatga cagtcaaaac aacagtttct acgaaagata tcgatgaggc
     5761 atttttaaga cttaaagata ttgtcaaaga aacaccttta caattagacc attacttatc
     5821 tcaaaagtat gattgtaaag tctatttaaa acgagaagat ttacaatggg tacgttcttt
     5881 taaattaaga ggtgcttaca acgctatttc tgttttatca gatgaagcta aaagtaaagg
     5941 tattacatgt gcaagtgcag gtaatcatgc tcaaggtgtt gcctatacag ctaaaaaact
     6001 taatttaaac gctgttatct ttatgccagt cactacacct ttacaaaagg taaatcaagt
     6061 aaagttcttt ggaaatagta acgttgaagt tgtactcact ggtgatacat ttgatcactg
     6121 tttagctgaa gctttaactt atacaagtga acatcaaatg aactttatag atccattcaa
     6181 taatgttcat acaatttctg gacaaggtac gcttgctaaa gaaatgctag aacaagcaaa
     6241 gtctgacaat gttaactttg attatctatt tgccgcaatt ggtggtggcg gtttaatttc
     6301 aggtattagt acttacttta aaacctattc acctaccacg aaaattatag gtgttgaacc
     6361 ttcaggtgca agtagtatgt atgaatctgt tgtggtaaat aatcaggtag tcacattgcc
     6421 taatatcgat aaatttgtgg acggtgcatc tgtagctaga gttggcgata ttacatttga
     6481 aattgcaaaa gaaaatgtag atgattacgt tcaagtagat gaaggtgcag tttgttctac
     6541 gattttagat atgtattcaa aacaagcaat tgtagcagaa cctgctggcg cattaagtgt
     6601 aagtgcgctt gaaaactata aagatcatat taaaggtaaa acagtggttt gtgtcattag
     6661 tggtggtaat aatgatatta atcgaatgaa agaaattgaa gaacgttcat tactatacga
     6721 agaaatgaag cattacttta tcttaaattt ccctcaacgt ccaggtgcat tgagagaatt
     6781 tgtaaatgac gtattaggac ctcaagacga tattactaaa tttgaatact taaaaaaatc
     6841 ttctcaaaat acaggtactg tcattattgg tattcaactt aaagatcatg atgatttaat
     6901 acaactcaaa caacgtgtaa atcatttcga tccttccaat atttatatta atgaaaataa
     6961 gatgttatat tcattgttaa tttaacacat agtaagaaaa acagtcataa attgatttct
     7021 aattgaaatc atcttatgac tgctttttat tatactttac atttctcgtt tcgtcagatt
     7081 caaacgtttt cacttcgcca agccatcttt ctttgtgttt gcttttattt tgacgtttta
     7141 gacataaaaa aagagacctt gcggtctcaa tgcggctcat cgcatccact ttttgcctgg
     7201 caacgttcta ctctagcgga acgtaagttc gactaccatc gacgctaagg agcttaactt
     7261 ctgtgttcgg catgggaaca ggtgtgacct ccttgctata gtcaccagac atatgaatgt
     7321 aa
//
LOCUS       DAKTIP010000133         7138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAKTIP010000133 DAKTIP010000000
VERSION     DAKTIP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.133"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(11..1372)
                     /locus_tag="O0305_001993"
     CDS             complement(11..1372)
                     /locus_tag="O0305_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0275132.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1720..2841
                     /locus_tag="O0305_001994"
     CDS             1720..2841
                     /locus_tag="O0305_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCY0275133.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2890..4158
                     /gene="icd"
                     /locus_tag="O0305_001995"
     CDS             2890..4158
                     /gene="icd"
                     /locus_tag="O0305_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCY0275134.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4548..5252
                     /locus_tag="O0305_001996"
     CDS             4548..5252
                     /locus_tag="O0305_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0275135.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5252..6913
                     /locus_tag="O0305_001997"
     CDS             5252..6913
                     /locus_tag="O0305_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCY0275136.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2571 a         1020 c         1333 g         2214 t
ORIGIN      
        1 aaacttgttg ttatttattt gatttagcag cgatacgttt atatcttaag tacataaatg
       61 ctaaaagtat aaaccaaatc ggaataaaat aaattgcacg tcttgtatca acattaataa
      121 ataataaccc gaacacaaaa atgaagaata caaatattaa gtagcccata tatttgccac
      181 ctaatagttt gtacgtagca tttttatgta gatctgggtt tttacgacta taattgatat
      241 atgcaatgat aatcagaccc catacaacta aaaataacac tgtagagatg gtagtcacat
      301 acgtaaatac ttttgtcgca tctgggaaaa tatagtttag taatgctgcc acaagtaata
      361 atgctgatga agcaaagatt gcaacatgtg gaacgccata tttattcgtc ttagaaaagt
      421 tcggaggtgc ttgttgttga cttgataaac cgaaaagcat acggctattt gagaatatac
      481 cactgttaca tgatgaagca gcagcggtta atactacaaa attaatcaag cccgcagcaa
      541 acggaattcc gatcaatgcg aataatttta cgaatggact gttatcagga tcaacttgct
      601 gccaagggat aatagacatg ataaccgcta acgccccaac gtaaaatatt aaaattctaa
      661 tgggtacact gttaattgct tttggaattg ttttaactgg atcttttgtt tccccagctg
      721 taacaccaat catttcaatt cctacaaatg agaatagtgc catttggaaa gacataaaga
      781 atccagaagc acctttagcg aatatgccgt gttcatatag atttgtgaaa ctggcatgcc
      841 cgaattgagt tttaaatgca aatagaatca tgacgaaacc aactactatt aaaccaataa
      901 ttgtcgctat tttaatgata gagaaccaaa attctaattc tccgaaaagt cttgcgctaa
      961 gtaggttgaa tgacattaat aacagtacac aaaatagtgc acttatccag tttggaattt
     1021 ctgggaacca aaagctaaca tattttgcca cagccgttac ttcagccata cctgtaataa
     1081 tccaacagaa ccagtatgtc catccggtaa caaatcctgc aaaaggccca atatatgtat
     1141 tggttacatc tgcgaaagat ttaaattcag tattctgtat aatgatttct cctaaacctc
     1201 gcataaacat aaataacata aatcctataa tgatgtatgt taatagaatt gaagggccgg
     1261 ttaatgcaat cgtttgacca gcacctaaga ataagcctgt accaattgca ccgccaattg
     1321 caattaattg tatgtggcga ttgctcagtt ccctttgtaa tttttcagcc ataatacatc
     1381 tcccttaaat atagatatgt ttattatgca cttatattga gatatataca attattttcg
     1441 gtaaaaatgt gtaaaattcc atgttaatat actttggttt ttataatcat atataataac
     1501 caattgaaaa tttaattcta ttgtaaaatt catggattat tcacatcttg aaaaagcttt
     1561 aatggtgcta tttgtggcta ttctgtgaca tttacataga tttacaaaaa aattgttgca
     1621 catataatgc cagtttttat atttcacaaa cgaaatgcgt ttagctataa tattagttga
     1681 aagccatttc ataaagaaac agtaaagggg aaatttatca tggcagaatt acaaagaggt
     1741 ttagaagggg ttatcgcagc ggagactaaa ataagttcaa ttattgaaag tcaattgact
     1801 tatgccggct atgatattga tgatctagct gaaaatgcgc aatttgaaga agttattttc
     1861 ctattatgga actatagatt gccaaacgaa gaagagcttg ctcatctaaa agggaaatta
     1921 aatcaataca tgacattaaa tcctcgtgtg tatacacatt ttgaggagta tgttacagat
     1981 cacgtgcatc caatgacagc attacgtacg tcattatcat atattgcaca tttcgatcct
     2041 gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag caatgcgtat acaggctaaa
     2101 gtagcatcat tagttacagc gtttgctcga gtaagacaag ataaagaacc acttaagcct
     2161 aatcctgact taagttatgc ggcaaacttc ctatatatgt tacgtgggga attaccaaca
     2221 gatatagaag tagaagcctt caataaagca cttattttac acgctgatca tgagttgaac
     2281 gcatctgcat ttacggcacg ttgtgcggta tcatcattgt cagatatgta ctcaggtatt
     2341 gtagcagccg taggttctct gaaagggcca ttacatggtg gtgcaaacga acaagttatg
     2401 acgatgttat ctgagattgg gtcaattgaa aatgttgatg cttacttaga tgaaaaattt
     2461 gctaataaag ataaagtaat gggcttcggt catcgtgtat ataaagatgg tgatcctaga
     2521 gcgaaatatt taagagaaat gagccgtcaa attacgaaag acgctggtcg tgaagaatta
     2581 tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag aaaaaggatt aattcctaat
     2641 gttgattttt atagtgcgag tgtttatcac tgtatggaaa tacctcatga cttattcacg
     2701 ccaatctttg ctgtaagtcg ttctgcagga tggattgctc atattttaga acaatataaa
     2761 gataatagaa ttatgcgtcc tagagcgaaa tatattggcg aaacgaatcg taagtatatc
     2821 ccgcttgaag aaagaaaata atcaatacaa attaaaaatg aagatgtaaa atttggaggt
     2881 aaaataacta tgactgcaga aaaaattact caaggaactg aaggattaaa cgtacctaat
     2941 gaaccaatta tcccatttat tatcggtgat ggaattggac cggatatttg gaaggcagca
     3001 agccgagtta tagatgctgc tgttgagaaa gcctataatg gcgaaaaacg cattgaatgg
     3061 aaagaagtgc tagctggcca aaaagcattt gatacaactg gtgaatggtt acctcaagaa
     3121 acacttgata caattaaaga atatttaatt gctgttaaag gacctttaac aacaccaatt
     3181 ggtggtggta ttagatcatt aaatgtggct ttacgccaag aattagattt atttacttgc
     3241 ttaagaccgg tacgttggtt taaaggagta ccatcacctg ttaaacgtcc acaagatgtt
     3301 gatatggtta ttttccgtga aaatactgaa gacatttatg ctggtattga atttaaagaa
     3361 ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa acgaaatggg tgcgacaaac
     3421 attcgattcc cagaaacttc aggtattggt attaaaccag tttctaaaga aggaactgag
     3481 cgattagtta gagcagctat acaatatgct atcgataata accgtaaatc agttacttta
     3541 gttcataaag gtaatattat gaaatttaca gaaggctcat ttaagcagtg gggttacgat
     3601 ttagcattat ctgaatttgg tgatcaagta ttcacttggc aacaatatga cgaaattgtt
     3661 gaaaatgaag gcagagatgc tgctaatgct gctcaagaaa aagctgaaaa agaaggcaag
     3721 attatcatta aagattctat tgctgacatt ttcttacaac aaattttaac tcgtccagct
     3781 gagcatgatg ttgtagcaac tatgaacttg aatggtgact atatttcaga tgctttagct
     3841 gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca ttaattatga aacaggtcat
     3901 gctatttttg aagcaacaca tggtacagct ccaaaatatg caggtttaaa taaagtgaat
     3961 ccatcttcag taattttaag ttctgtatta atgttagaac atttaggatg gcaagaagcg
     4021 gcagataaga ttacagattc aattgaagat acaattgctt caaaagttgt tacttatgac
     4081 tttgcccgtt taatggatgg tgctgaagaa gtttctacat cagcatttgc agatgaattg
     4141 attaaaaatt taaaataagc aaaataaaat taggtgagtc tagcaacttt aattgctagg
     4201 ttcgcctttt taaatgaatt gaagttggca acaggataga aataataaag agtcaccaat
     4261 gattgattat gtagtgtttc ttacacatta gacaaagcga atatgtacat aagcttagga
     4321 ataaatgagt aaaactcatg cataggtagt gggatagaaa tgctattttt acaaaattta
     4381 tttcgttgtc ccagagtaag tcatgcaaat gatagaagga aatataagaa gtattttatt
     4441 tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg taggatgtta tttttataaa
     4501 tagttcggtt ataatgtaaa tataattata agttagggag gcataccatg tcgcaaaaag
     4561 tgttggtagt agatgacgaa cattcaatcg taacattact taaatataac ttagaaacag
     4621 ctggttatga agttgttgtc gcatttgatg gtgatgaggc tttagaaaag gtagaaagtg
     4681 aacagccaga tttaattatt ttagatgtta tgctacctaa aaaagatggc attgacgtat
     4741 gtaagactgt aagaacaaat aaaaatttag tccctatatt aatgttaacg gcgaaaaatg
     4801 atgaatttga tcgggtatta ggtttagaat taggtgccga tgactatatg actaagcctt
     4861 tttcacctag agaagttgtt gcccgtgtga aagccatttt aagacgttct caatttgtaa
     4921 aagaagtaga agagattgac gatgatatta ccatcggatc gatacgtatt agacccgatt
     4981 attttgaagt atataagcat aatgagttat tggagctaac accgaaagaa tttgaactct
     5041 tgctttattt gatagaaaga caaggcagag tcattacgag agagcatatg ttaaatacgg
     5101 tatggaatta tgaatttgca ggagactcaa gaattgttga tgtacatatc agtcatttaa
     5161 gagataaatt agaagataat cctaagaagc cacaacttat taaaactgtg cgtggcttag
     5221 gctataaatt ggaaagacct aaagaacaat gatgaagttt caccaccgct taatgttatt
     5281 gataagcagt atattaatta tcagttttgt tacattaggc attattattc acgcttccat
     5341 cacatatgtt ttatcagaaa aacacgaaca agctctagca acagatgcaa gatcatatat
     5401 ttatttagtc caagataatg agattgataa agttaaaaaa attgtaaaac agcaaaatat
     5461 agatttattg ataactaagc atgataaaaa ggtgtttagt agtggcaaac tgaaagctat
     5521 cccaaatagt gatagctata ttcaaaaaag ggacgttttt atttttaata agaaggttaa
     5581 cggttatcat gtatggatta aaggttacaa taacgatatt acagaaatgc attggacgtt
     5641 gtggaaatat cttattttga catgtttagt tgtactcatt tgtctgtatt ttgcaagccg
     5701 tagttttaag cgtacattaa tccgtcccat tcaagaagtt acttatgcaa cacaattgtt
     5761 ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat gttgtagaaa ctaaagcgct
     5821 ttttgtgtct accaatgatt tagcacgacg ccttcaaaag cttaataatg agcaaaaaat
     5881 tcaatccaac cgtttaaaaa caacgattga aaatattcca agtgctattt taatgataga
     5941 tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat gaacagttca acatctcgca
     6001 taatattgaa caagtaggct atcacggcta tgttaatact gaaattgagc aattaatttt
     6061 agaaagtttt aaagttgaaa aacctattta tgaacaattg gaagttgcaa taaaccaagt
     6121 ccatgccaag tactttgata tttcatgcgt tccgatttta actaggtctc aaaaaagttt
     6181 gcaagggatt ttagttgtga tgcatgatat aacaaatctg aaacaacttg aaaatctaag
     6241 acgtgaattt gtagcaaatg tttcacatga attaaaaaca cctattactt caattaaggg
     6301 gtttgctgaa acgcttattg atggtgcaaa aaatgatgcg gaatcattag acatgttttt
     6361 aaacataatt ttaaaagaat caaatcgaat agaatcttta gtaacagatc tgttagattt
     6421 atcgcatatt gagcaacata cagagttaga tacagactat atgaatttat cagatttaac
     6481 gcggcgtatt attgataata tgatgactca agccaatcaa aaaaatattt ccattcatac
     6541 tgatattgaa aaagatgtca ttgttaaagc acaagaaagt aaaattgctc aagttatcac
     6601 gaatttgtta acgaatgcaa ttaactattc ttatgaagat ggagatatta acgttcgtgt
     6661 gtatcgagat gactttcgtg tcattttcga agtacaagat tttggtatag gtattaaatt
     6721 agaagaccaa caacgtattt ttgaaagatt ttatagagta gataaagcac gaagtagaga
     6781 ttctggtggg acaggtcttg gattatcaat tacaaagcat atagtagagg cccaccaagg
     6841 caatattgaa gtgaatagtc aagttggcaa aggctcaacg ttcaaagtta ttctaaaaga
     6901 ttataaagaa taaaaattaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg
     6961 cttttttatt tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaataaa
     7021 ttcggttgtc aattttgtca tttgtatatg tcgatttgta agttataagt aatagattgc
     7081 tgtgaatcaa gcaattttat aatatattta ttataataaa gaaattttag taacgcgc
//
LOCUS       DAKTIP010000134         7097 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAKTIP010000134 DAKTIP010000000
VERSION     DAKTIP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.134"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..207)
                     /locus_tag="O0305_001998"
     CDS             complement(<1..207)
                     /locus_tag="O0305_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0275137.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVF"
     gene            complement(218..910)
                     /locus_tag="O0305_001999"
     CDS             complement(218..910)
                     /locus_tag="O0305_001999"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCY0275138.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFAAAKIGYHRLFDIKPVNTRYGGRIIFVDDCIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(921..2036)
                     /locus_tag="O0305_002000"
     CDS             complement(921..2036)
                     /locus_tag="O0305_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCY0275139.1"
                     /translation="MKFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(2014..3021)
                     /gene="bioB"
                     /locus_tag="O0305_002001"
     CDS             complement(2014..3021)
                     /gene="bioB"
                     /locus_tag="O0305_002001"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCY0275140.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNEVSRA
                     N"
     gene            complement(3023..4381)
                     /gene="bioA"
                     /locus_tag="O0305_002002"
     CDS             complement(3023..4381)
                     /gene="bioA"
                     /locus_tag="O0305_002002"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCY0275141.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(4359..5045)
                     /gene="bioD"
                     /locus_tag="O0305_002003"
     CDS             complement(4359..5045)
                     /gene="bioD"
                     /locus_tag="O0305_002003"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCY0275142.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(5099..5266)
                     /locus_tag="O0305_002004"
     CDS             complement(5099..5266)
                     /locus_tag="O0305_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275143.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(5497..>7097)
                     /locus_tag="O0305_002005"
     CDS             complement(5497..>7097)
                     /locus_tag="O0305_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275144.1"
                     /translation="NVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYE
                     MSAKERLDIGDKLKNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILI
                     TVLILSLLVVSWQVSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVI
                     RGIQVIKSFAKENTSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLV
                     ACLLYMNHSIDLPTLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPEL
                     DENGKNLTIKNENIAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHL
                     LLRFYDIDDGNIRIDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATK
                     EEIIRAAKQACCHDFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILD
                     EATASIDPENEQLIQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHD
                     ELIRKPGMYQDFIRIKSKSAGWKL"
BASE COUNT         2219 a         1344 c          915 g         2619 t
ORIGIN      
        1 aaatacgcca aatactagta ataataggct agataataat ccacctaaaa agtacttttt
       61 aattccaaag aaagctaata tcaataatgc tgccggtgca gataattgaa aatctaatcc
      121 tggtataatg gacggtattt tcaaaactgc caaaatggtt aaaatcgcag caatgacact
      181 aatttgagta atatcttttg atgtcatact aaaaccccta taccgtttca taaacaactt
      241 gcttcggtgt gctttctaaa aatgatatgt aatgatttaa atcaatacaa tcgtccacaa
      301 atattattct gcctccatat ctcgtattaa ctggtttaat atcaaataat cgatggtaac
      361 caattttagc agcggcaaaa taacctgtcg tatacgttaa gtcatcggac acgcaaagtt
      421 ctcctttgac atacggatgc gcattgatac aactagcaat tgctaaggca tcagtcactc
      481 tttcattaag atcaccttta ttatttatat cttcaaacga aaaatgtgtt gccctaatcc
      541 ccctttgtcc aaatgaatct aaacgtttac cagatatagc agatagaata atagctcctg
      601 tataaaccgt ttcattttta atatatgtca tcccttgatt tagcgcttgt tcagtgacac
      661 cacattcttg tgttaaatgt tggagatttg ctttatcatc ctcaataatt tgtaatgctt
      721 ttatttgttg aatcggttcc atgatttttt gtatttttat attgagaaaa tccggttgcc
      781 cattttcatg aaaaaaacct ttattataaa acctttgtac cgtctgttct attttgtcaa
      841 actcacaaat cgtttcagca ccactaatat gaacatcttg attgctagaa cgcattttta
      901 tactatacat gacgatcacc tcaatcttct tgatgcaaaa tttcaaacaa cctatctata
      961 tcttgttcag tatgaaaata cgacaatgat attcttaaca ttggcttagt cacagttgga
     1021 taccttaaat aacttgtaaa cacatgatgc tttaataatg tttgatgaat gttctcagcc
     1081 gcttctatgt catcaaactc aataaactta atcggcgagt ttgaactatt ataattaaca
     1141 ttgagtgctt ttaacttttg gttaaaatat ttactcaaac tatttaattt agtgcgtcta
     1201 tcatcagcat ttattaactt ttcaatgttt ctttttataa aatacaaatt ataaattggc
     1261 aaactacttg agtagatgag tggtctaccg tgattaatta acatatcctt cacatcattt
     1321 gaacttaaaa tcacaccccc gtatgcacca catgctttag ataaactaga agtgagtata
     1381 tctacacctt gataattcga gtaattctct attccaaaac tatgtgaaac atcgagtatc
     1441 agtgttgcgt tatatttatg ctttaatgag actaattgac caatatccac aacgtcgcca
     1501 ttcgttgaaa atacactatc agatatgatt atttttggta tattttgatt agggtatttc
     1561 tctaaccttt tttctaaatc agcaatatct aaatgcttat atatcacttt ttctaaacca
     1621 cttaacttaa taccgtcaat aatactcgca tgattttctt gatctgaaaa cacgacacaa
     1681 tttgtatttt tgaaaatatt aaataacgcc aaattagcat cataaccact atttaagata
     1741 gtacatgcac tatatccgag ccaacctgct aacattgttt caatttcttc ataagctgtc
     1801 gaacttccac taattaatct tgaacttgat aagtgataac tatacttccg cataaatctt
     1861 tcgaaatcat ccttatcaaa cgctatttga cctaatccta aataatcatt agatgtatag
     1921 ttcgtacatc tcttattttc tacttcaata tactgtctat ctatataccc taccgattta
     1981 agcgaccgat ataacccttt ctgttgtaat aaatcaattt gctcttgaaa cttcattctt
     2041 gttttcctta ttttcacaag tgtcataatc aatttcaaag cctaaatcat taatcatatc
     2101 gtagtctaat tggttcggtt gcccaccagt aattagataa tcaccgacaa atattgaatt
     2161 cgccgctttt aatgctaatg gctgtaacga acgtaagttg acctctcttc ctccagcaat
     2221 acgaatttct ttcgtaggat tgattaatcg gaataatgct acgattctta aacatttcat
     2281 tggtgttaaa tcatccatgc ttccaaactt tgtgcctttg attggatgca aaaaattaat
     2341 cggaatactg tcggcatcca tttcttttaa agcaaatgcc atatcaacaa tatcttgatt
     2401 agattctccc ataccacaaa tcacgccaga acatggtgat atattattcg ctttcattag
     2461 ttctatcgta tctgttctat ctttataact atgcgttgtc acgacgttat catggtaatt
     2521 ttcacttgta ttaatattgt ggttatatct gtctacacca gctgacttaa gtttcttagc
     2581 ttgttcgtca ttcgttaatc ctaaacatgc acagattttt agttgcgggt gttgagattt
     2641 aatcgttctt acagtattac taatatgatc aacttcttta tcgctcggtc ctctaccact
     2701 cataacaata caatatgttc caatatgatt atcatgtgcc acctttgctc catcgataat
     2761 ttgttcctct ggaattaaag catatcgctg tttttgttta atatctcgtg attgtccaca
     2821 gtacccacaa ttttcaggac atataccact tttagcattt aaaatcatgt ttaattttac
     2881 ttttttacca aaataatgtt ttcttaaaat gtacgcctca tttaataaat ctaaggtatc
     2941 aatattagta tcctcataaa ttttcaatac agtctctttt gttaattgtt ccccttgtaa
     3001 tatgcgttta gccaaattca tattaacact tcctatctaa aatgttatgt actgcctttt
     3061 taaaaatacg taccatcgtt ttcacttctt tatttgacat acttaacact ggcacaaacg
     3121 taatgacatt ttctaaattt ctaatcatta gcccgttttc tttacaatta cgtacaatac
     3181 gttcaacttt ttcaatttct aacggcgttt tactatcttt atctgtaact aattcaactc
     3241 caaacattaa gcctcgtcct cttacatcac caacatttcg atgacgcttc agcgcatgta
     3301 actgtttctc aagtgttgat gatgtcgttt caatatgtga caataactta cgtttttcat
     3361 aaagtcttat attttctaat gcaaccgtac aaacgatttg atttccggtg tatgtatgac
     3421 catggaaaaa ggtattcaca ccatgcgaat cacttaaaaa tgcattgtat atttttttag
     3481 atgtcaatgt agctgcaagt ggtaagtagc caccagtaat cgccttacct aaacacataa
     3541 tatccggttg aacatcttca tgattgcatg caaacatctt tccagttctc ccaaaaccaa
     3601 ctgctacctc atcacaaatt aataagacat cgtatttttt gcacaatttc tcgacttctt
     3661 tcaaaaagcc tttaggatga acaaacaagc ctgtcgcacc ttgaatcaac ggttccaata
     3721 taaaccctgc gatttcatca tttctttcaa ttagaatttg ctctatattc gttaaaatag
     3781 ctgtcatcat ttcattttca gtatcgtaat tactgcgata gaaagatggg ctttctactt
     3841 gaatattctc aaatattaag tctttaaata ttttatgaaa ggtcttgata ccaccaacac
     3901 ttaccgcacc aatcgtatcc ccatgataac cgtgatttag cgttataaac ttgtttttct
     3961 tggcatattt ttctctatca atatttttcc aatactgata tgccatcttt attgcgattt
     4021 caacagacgc actgcctgta tcagaataaa atacttttct tagattactt ggcgtgattt
     4081 cgattaattt ttccgcaagt tctattgacg gaatatttga tgatcctagc agcgtagaat
     4141 gggcaatttt attgagttgc tttttaatta ccttatttaa gtatttgtta ttatgaccat
     4201 gcacattgac ccacaacgat gcataaccat ctaaatattt attgccattc gtatcgtaaa
     4261 ggtaactacc ctttcctttt tcaatgatga ttgcttcttc tttgctatat acacccattt
     4321 gtgtaaatgg atgccaaaca tattctgagt ctttttgttt aagttgttgt gtgtaattca
     4381 tttttaactc ctattaattg ttttaaaaat gcttctgaga aattttcata cgtggcatgt
     4441 tcttcaaatg tatagactgt tttatttgtt aattttccaa tcgtcatttg attgtctttt
     4501 tcaatatagc tgtctgtata gcgattcatt attaaaaaat tactcgccga tacattctga
     4561 ttaacataat cttggtgaac aatggcatcg ctaatagcac ctaattttga tggcaacaca
     4621 ctgatgacac aatctgcaca atcattgatt agatctttag tcatgtagaa atcatctgta
     4681 ccttcatata ttggtacggc aattccccca gcaccctcaa ttaagataaa atcaaattcc
     4741 ttatctaaaa cttttacctt atctaataca cgctgcttat ttagaaaaat ttgatctgtc
     4801 attttaaatg caaggtgtgg tgatacaggt tgcttaaaag tataaagtga cgttatgtca
     4861 tagcttaaat cacattcatt tttaaatact tctaaatctg gaaacgtccc gtcttggcgt
     4921 tcctcagttt gaaatggttt aaaaatacaa actcgatgac cacgtgtttt caaagcatgg
     4981 tataaatgct ttgtaacata ggttttgcct acatcagtat tcgtacttgt aataaaaatc
     5041 ctcataaaaa taaccacctc aatccaaatg taaactttta taattaataa gtttacattt
     5101 aaggcgcatg tttttcaaga aaacatgaat ttaaacttct cagtataact ataaattttg
     5161 gcaaaatttt gaccttgcta ttgaaatgac taagacttct taaggaattc gggcagatat
     5221 aaacagcgat tcgataaaat atgtcagtta aaattaataa ttgcattatt tcttaataaa
     5281 aagacatcct agctttacca tcttgtgaaa caatccatta attgtaatat tacgttgagc
     5341 gccttgttca tcaatgctat aattagttac ataataaatt gaacatctaa atacaccaaa
     5401 tcccctcact attgccctag tgaggggatt tattttgctg ttgattatct atcacccttt
     5461 ttatttccaa attctgattc catattaaac tattatttat aatttccagc ctgctgattt
     5521 actttttatt cttataaagt cttgatacat tcctggtttt cgaattaatt catcatgact
     5581 acctttttga attatttcac cttcattgag cactataatc tgatctgcat ttttaatagt
     5641 ttcaagttta tgtgcaattg taattactgt tttgccttta cttaattcat taattgccgt
     5701 ttgaatcagc tgttcatttt cagggtcaat acttgcagtt gcttcatcta aaataattat
     5761 tggtgcatct tttaatatcg ccctagcaat agaaatcctt tgcttttctc cgccagataa
     5821 attactacct ttttcattta gcattgtttg atatccctca ggtaatgaca tgatgaagtc
     5881 gtggcaacat gcttgcttcg ctgcacgaat aatttcttct ttcgttgcac ctggattgcc
     5941 aaacaatatg ttattttcaa tcgtatcatt aaataaatac accttttgaa atactgcact
     6001 aattttcgac attaacgtac ttaatgtcat atctttaata tcaacaccat cgatgcgaat
     6061 atttccatca tcgatatcat aaaagcgcaa gagtaagtga cataatgtag atttcccact
     6121 tcctgaaggt ccaattattg ctgttgatgt ttgtgtaggt atctcaaaat tcacattctt
     6181 tatcacttgt ttatcatcat atgaaaagtt cacgttttga aaagcgatat tttcattttt
     6241 aatcgtcaaa ttttttccat tctcatccag ttctggagca ttttttatct tttcaatatc
     6301 atctatcgtc atatctatca tttcaagtac gtgtgcagca ctattaatat tttcaacact
     6361 atcaaatatc acaaatgaaa aaattgaaat cataataagg gtaggtaaat caatactatg
     6421 attcatatat aataagcatg caactaatac aatcattatt gaaacaactt ttaaactaag
     6481 taaatgcaat aaattgaatg ggatatattg catttctatt tttgtattta tacgtttact
     6541 ttcattgact gcttggttaa aacttttaag actcgtattt tctttcgcga atgattttat
     6601 tacttgaata ccacgaataa cctccaatac cttttccact aattggtttt gtacattatg
     6661 atacgctggc gcattttgtc gactctttct ttctaataat tgaatcgcaa aaaatgatag
     6721 taatacgcca atgcatgcta ataatgatac ttgccacgaa actacaagta gagacaatat
     6781 gagtactgta attaatatgt atccattaac aacaacgtcc accattttca tagcaaagtt
     6841 ttctaaaaag gttaaatctg ttgttactat tgttgttaac tcatttgaat gatgcgaatt
     6901 aaaataacct aaccttacat ttttcaattt atcccctata tctaaacgtt ctttcgcact
     6961 catttcataa gcgatgctct catggctttt gcttttgaaa tatgctgtaa taaatcgtcc
     7021 aatcactaat aaaaccatga taattacaac attcaatata tctttcatat aaataggttt
     7081 atgagatagc acattat
//
LOCUS       DAKTIP010000135         7037 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAKTIP010000135 DAKTIP010000000
VERSION     DAKTIP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.135"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..169
                     /locus_tag="O0305_002006"
     CDS             <1..169
                     /locus_tag="O0305_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HCY0275145.1"
                     /translation="CYASKKIKRHRQNEFIFILNDSKKEINEHNKNLLHENYISTIDY
                     SELDEKIGLLI"
     gene            630..838
                     /locus_tag="O0305_002007"
                     /pseudo
     CDS             630..838
                     /locus_tag="O0305_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1412..1500)
                     /locus_tag="O0305_002008"
     tRNA            complement(1412..1500)
                     /locus_tag="O0305_002008"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1463..1465),aa:Ser,seq:gga)
     gene            complement(1555..1626)
                     /locus_tag="O0305_002009"
     tRNA            complement(1555..1626)
                     /locus_tag="O0305_002009"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1591..1593),aa:Glu,seq:ttc)
     gene            complement(1628..1702)
                     /locus_tag="O0305_002010"
     tRNA            complement(1628..1702)
                     /locus_tag="O0305_002010"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1668..1670),aa:Asn,seq:gtt)
     gene            complement(1717..1790)
                     /locus_tag="O0305_002011"
     tRNA            complement(1717..1790)
                     /locus_tag="O0305_002011"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1756..1758),aa:Gly,seq:tcc)
     gene            complement(1806..1881)
                     /locus_tag="O0305_002012"
     tRNA            complement(1806..1881)
                     /locus_tag="O0305_002012"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:His,seq:gtg)
     gene            complement(1895..1967)
                     /locus_tag="O0305_002013"
     tRNA            complement(1895..1967)
                     /locus_tag="O0305_002013"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1932..1934),aa:Phe,seq:gaa)
     gene            complement(1984..2059)
                     /locus_tag="O0305_002014"
     tRNA            complement(1984..2059)
                     /locus_tag="O0305_002014"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2024..2026),aa:Asp,seq:gtc)
     gene            complement(2081..2154)
                     /locus_tag="O0305_002015"
     tRNA            complement(2081..2154)
                     /locus_tag="O0305_002015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2118..2120),aa:Met,seq:cat)
     gene            complement(2626..3180)
                     /locus_tag="O0305_002016"
     CDS             complement(2626..3180)
                     /locus_tag="O0305_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCY0275146.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(3503..4903)
                     /locus_tag="O0305_002017"
     CDS             complement(3503..4903)
                     /locus_tag="O0305_002017"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCY0275147.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGFDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYNDQLEEVY
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTHDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(4927..5850)
                     /gene="hemH"
                     /locus_tag="O0305_002018"
     CDS             complement(4927..5850)
                     /gene="hemH"
                     /locus_tag="O0305_002018"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCY0275148.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(5908..6945)
                     /gene="hemE"
                     /locus_tag="O0305_002019"
     CDS             complement(5908..6945)
                     /gene="hemE"
                     /locus_tag="O0305_002019"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCY0275149.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(6935..>7037)
                     /locus_tag="O0305_002020"
     CDS             complement(6935..>7037)
                     /locus_tag="O0305_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275150.1"
                     /translation="CQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
BASE COUNT         2283 a         1352 c         1025 g         2377 t
ORIGIN      
        1 atgctatgca agcaaaaaaa taaaaagaca caggcaaaat gagtttattt ttattttaaa
       61 tgactcaaaa aaagaaataa atgaacataa taaaaatctt cttcatgaaa actatattag
      121 tacaatagat tatagcgaat tagatgaaaa gataggttta ttgatttaat atatatagac
      181 gtgataatat caatgtttat attaaatgaa acgaatttga aaatttcgaa acagcttaag
      241 atagcaaatt gaatagcctt attgataatg cagaatcgtc tacacttagt tgaacaaatt
      301 ctatgagaat agatattgtt aaactatttg ggtaggcgat tttattttga caagagtcag
      361 aagattattt agttcatagt ttaagttaca aatggctaaa ctttatgaaa atggtatgac
      421 tagaatcgaa attatactcg aatacgattt aacactctca atcttctcga actgaataaa
      481 tactgggtca ttcaatcatc aagacaactt aataagtgat gataaatgtt cataccattt
      541 tgcaaatata accagatact ctcaacaaaa tgtagagttc aaaattaaaa atgtccctaa
      601 ttggcacatt gctgtatgat aataaattca acttagattt caacaatata actaatgatg
      661 cttatattta ccgcgatatc aaaccaaaca gcccatccac aaacaaatta gaatatatca
      721 tgttaataat agtaaatgat atatcttcca aagaccgcaa actgtagcag aacatgagaa
      781 ccttgcgatt ttgaaatggt ctaataaatc aaatatcatt gaaactttaa tcacataaat
      841 taacagtagt cacctgttcg cagttctata aattacaaat gcaataaagg ctaaagtaaa
      901 ctaattactt tagcctttat taataattta gtcgaattta ttaaaaatcg actaactcct
      961 ggttatgcta cgctttactt tataatccgc acgtagatgc cgattgatta tattataata
     1021 ttattatttt attttttcaa gcgtatatat aatatttgat tttctttaag taacagctaa
     1081 acattacatt tcaaaacgat taggtcatgc agatgtacat acaacgctta aaacatattc
     1141 acatttgatt aaagaatttg aagataatga aactaaattt atagaaaaaa atctgaatga
     1201 tttgttttgt gactaaactt caaaatcttg tgactaaaat taggattatt catcaaaaat
     1261 tagtcacatt gtggctaaaa aattttaaat ggatataaat aaataagaag tgtaaaaatg
     1321 aggataatag ctcaaatgca gatatatcaa gggattgagt ccaaaataaa aatagcgctt
     1381 aaaatgggta aaaccaattt aagcgccttt acggaaggta agggattcga acccttgaga
     1441 cgcttgtgac gcctacacac tttccaggcg tgctccttcg gccagctcgg acaaccttcc
     1501 tcaacaataa atatgtaata aaaaaacaga agcgatattt cacttctgct tgtatgactc
     1561 ctacgggact cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa
     1621 ggagccatgg ctccacaggt aggactcgaa cctacgaccg atcggttaac agccgatagc
     1681 tctaccactg agctactgtg gaataataga aaataatgga gcgggtgatg ggaatcgaac
     1741 ccacaacatc agcttggaag gctgaggttt tgccattaaa ctacacccgc taatgtaaac
     1801 gattatgggg cggctgaagg ggatcgaacc ctcgaatgtc ggaaccacaa tccgatgtgt
     1861 taaccacttc accacaaccg ccatgaatta agaatggttc aggacagagt cgaactgccg
     1921 acacatggag cttcaatcca ttgctctacc aactgagcta ctgaaccgta atggtaataa
     1981 taatggcggt ctcgacggga atcgaacccg cgatctcctg cgtgacaggc aggcgtgtta
     2041 accgctacac tacgagacct attaaattaa aaactatgta ttgcgggagg cggatttgaa
     2101 ccaccgacct tcgggttatg agcccgacga gctaccgaac tgctctatcc cgcgctaata
     2161 ttattttgaa ttacctaatt aatataccat aatcaaaaac ctaaagtcaa gaactttttg
     2221 aatttaattt aaatgttatc tcttaaataa ttacttaaat atcgtagcaa catgttctct
     2281 gttgaacaca aatattagta tattcatttt tgtagtgtac gtcaacgaca ttttcaaagt
     2341 tttttgtgta aaaaaacgct tcttattccc ttttatcata taagtgtcta atagttgtca
     2401 taaatagtgt taaagcattt aaaaaggtat aggagttata aagtttacaa cgcctatacc
     2461 ttctgaaaaa gaaattataa aagcttgtta caccgcatat tctttcagtc agcgactacc
     2521 aatataacat tgtagcccta agacattgct tgacgcctca attacaacaa tttttcaaaa
     2581 tcagcagcta cctactgaca caacataaca caacccatac actaactatc gtgtcatgta
     2641 atcttgcatc cgatcttgca acgctgtaaa tgtttcgaag ccatcctctt ctaagaagtg
     2701 ccctccatct tccacgattc gcaagttccc ctctaatgca ttcattaaac gctgggtctc
     2761 tttatatgaa acgtatttgt catttttaga actcaatccg taaaaattgt caactttctt
     2821 tttaatatta tcgtaatcaa tggttacatt acttaaatca atatctaaat ctatattttc
     2881 tgcatcttct ttaaagcccg ctatactgaa aaagccttca atcggctgat caatcatttc
     2941 aatatatttt aaagctgtga ttgaacctaa accatgtgtt acaaaatatg tatccttttt
     3001 gcgtacatta atttgtttcg tcatagcttc aatccactga tccactgtct tcgcttcagg
     3061 ggattcaaaa ttaaataatg ttacgtcata tccttctaaa gttaagttat gctccaacca
     3121 ctgataccaa tgatttctac tatttccatg catagaatgt acaataatta catctgtcat
     3181 ctcattctct cctttcaact tactacttct tttctatttt taaaaaaatg actgattacc
     3241 tataattgta aaataaaaac accttaatta gaaatgttat atcgcaaagt gacatttcta
     3301 attaaagtgt attgtcatca tttcaatatc attcaaaaac agctaaacct ttgtctctgc
     3361 ttcaatttca caaaaataat tcccgctgaa agtatctata tttacacatt acttccacca
     3421 ttatataact taaaaatgac tatatttcat caaacattat ctaaaggcgt cgcacctaca
     3481 ccaacaccat ccaacaatta acttacaact ctgcgattac ttcttcagca gcaactttac
     3541 cttgcgtaat acaatcaggt agtccaaccg cttcaaaaga tgcaccagtt actctaagtc
     3601 gtggatatgt ttgtttaata tgtgcttgaa tctgtctaat ttgttgaata tgaccgacat
     3661 ggtactgtgg catacttttc ggcaaacgat tgacaattgt aaattcagga tcacctttaa
     3721 atgtcatcat ttgacttaaa tctctacgta caatcgatac taattcatta tctgtatgat
     3781 catcaaccac agtatcacct ggtttaccta catacgcacg aatcaaaacc ttaccttccg
     3841 gtgtagtaaa tggccatttt ttcgatgtcc aagtacatgc ggtaatgtct gtatcactcg
     3901 ttctcgcaat tacgaagcca gtaccatcat gggtattttc aatgtctttt tcatcaaatg
     3961 ccaatacaac agttgcaaca gtcgtactat ccatcgtttt aaagtaatca aatgctggat
     4021 cttgtccgaa ccaatttaaa aacacttgat gtggtgtcgt tactaatacg ccatcataca
     4081 cttcttctag ttgatcattg taaacaattt tatattgttt ttgagatgta attatatcat
     4141 ccactgacgt attgtagcgt attgtcacac ctttattttt aacatcttgt tctaatgctt
     4201 caataaatga gcttaaacca tgcttaaatt gtttgaattg tcctttcggt gcgccaggat
     4261 ataattgtct ttgtttcaga cgcttatttt tctcatcctt catacctttt atcagacttc
     4321 cgaatgcctc ttctttttct ttaaaattag gaaacgtact catcaaactt aatttatcaa
     4381 tatcggtacc ataaatacca cccattaaag gctctattaa attctcaagt acctcattac
     4441 ctaatcttgc tctgaaaaat gcaccaacag aaatgtcacc atcctgcatt tgagtaggtt
     4501 tttttaataa atcaaaccct gctcttaatt taccaagtgg cgatattaat tttgtagtaa
     4561 caaatggttt aatatctgtt ggaataccca taattgaacc acctggaatc ggatataatt
     4621 tatttttcgc aaaaatatat gattgtccag tcgtatttgt aacaatatct tgttctaatc
     4681 caatatcttt cgctaattct gtcataatcg tttttctacc taaataagat tcaggcccta
     4741 gttcaatcat ataaccatct ttacgatacg attgaatctt tccccccgga cgattcgatg
     4801 cttcaaagat ggttacatca atattaggat cttgctgttt taaaaaatat gcacttgata
     4861 aacctgttat ccccgctcct ataatagcca ctgatttagt cacaacgttt cacgcttctt
     4921 tcgtcattaa aatatagact tgatttcatc aacaattgca ccgataaata atggatgtgt
     4981 attcggcatt tttggacgat aataattcgc accaatatca tcgcaaacta ctttacattc
     5041 ataatcattg tcataaagca cctctaaatg ctcacataca aaacctactg gcgtatatat
     5101 aaagttttta tactgatgtt tttcatataa atcacgtgtt aaatcttgta catctggccc
     5161 taaccaaggt gtacctgtat taccttcaga ttgccaacca atcgcgatat gttcaatatt
     5221 agattgttct ttaattaaaa gcgcagtatg ttctagttct tgtggatatg gatcattatt
     5281 cttttcgatt aaaccttttg gcaaactatg tgccgaaaca actaataccg tgtctttatg
     5341 ttcctcttcc ggtatttgag ctaatgtttc gttgacttta ttcgtccaat attcaataaa
     5401 tttaggttgt tcataataat gtttcacatg tgtaagttga ataccatatt ttgcagcttc
     5461 ttcatcagca cgtttgtcat atgatcctac tgaaaatgaa gaataatgtg gtgctagtac
     5521 taccgtgatt gcttcagtaa tgccatcatt gtgcatttgt tcaaccgcat cttcgataaa
     5581 tggtgaaatg tgttttaatc ctaagtatag tttaaattca acatctgcat atgctttatt
     5641 taatgctgaa actagcgcat cagcctggtc atctgttgta cctgctaatg gtgataaacc
     5701 acctataaat tcatatctat ctttcaaatc ttgaagttct tcttcagatg gacgtttacc
     5761 atgtctaata tctgtataat atggctctat gtcactttct ttataaggtg tgccataagc
     5821 cataactaat aatcccattt ttttagtcat tgataatacc ttcctttaaa tgaattatct
     5881 ttcatgtgct tcaatgtaaa actatgatta tctttgtgta tatgtgtgta cgaattcgct
     5941 tactttacgt aacgtctctg gttgcacttc tgggaataca ccgtgtccta aattaaagat
     6001 gtgtttaccg ttctccatac cttgatctaa tattggtttc aatctctctt caatgacatt
     6061 ccatggtgct aataaaattg atggatctaa attcccttgt aatgttttag taacgcctaa
     6121 ttgttgagcc tgattaatag acgttctcca atctaggcct aatacatcaa tcggtaaatc
     6181 attccattca ttgattaaat gactggcacc tacaccgaat aaaattaccg gcacatcatg
     6241 tttttcttta acctcactga ttaatcgaat catatgtggt ttaatgtaac gtctgtaatc
     6301 ctcgacattt aatgcaccta cccatgaatc gaaaatttga atcaattcgg cacctgcttc
     6361 gacttgagct gttacatatt taacagatac atcaactaaa tgattcatta aagcaaacca
     6421 tgttgcttca tctctataca tcatcgcttt tgtaaaattg taatttttcg atggtccgcc
     6481 ttcaatcata tatgacgcta atgtaaatgg tgccccagta aatcctatta gcggcacatt
     6541 taacttttct tctgttaaaa gtttaattgt atctaataca tatggtacat ctcgttcggg
     6601 gtctatttga gaaagtttct caacatcttg aattgttttg attggattat gaatcactgg
     6661 accaataccc gatttaattt ctacatcgac accaattggc tttaatggtg tcataatatc
     6721 tttgtataaa attgctgcat ctgtatgata attatcaact ggtaaatgtg ttacataagc
     6781 gcacaactcc ggctgatgtg taatatcgaa tagtgaatat ttttctttca attttcgata
     6841 ttctggttgc gaacggccag cttgtcgcat aaaccaaaca ggtgtatgtg atgtttcttc
     6901 acctttgatc atttttaaaa ttgtattgtt tttattatgc accataaagg cctcctaaat
     6961 taaaatcatt cttatctata ttatcatatc gctcattcgt tcgtattttc aataaataaa
     7021 tgtcataaaa ctgacat
//
LOCUS       DAKTIP010000136         6971 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAKTIP010000136 DAKTIP010000000
VERSION     DAKTIP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.136"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(155..1060)
                     /gene="yut"
                     /locus_tag="O0305_002021"
     CDS             complement(155..1060)
                     /gene="yut"
                     /locus_tag="O0305_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCY0275151.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKATLLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            1320..1622
                     /locus_tag="O0305_002022"
     CDS             1320..1622
                     /locus_tag="O0305_002022"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCY0275152.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            1636..2046
                     /locus_tag="O0305_002023"
     CDS             1636..2046
                     /locus_tag="O0305_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCY0275153.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGATVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            2043..3758
                     /gene="ureC"
                     /locus_tag="O0305_002024"
     CDS             2043..3758
                     /gene="ureC"
                     /locus_tag="O0305_002024"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCY0275154.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            3771..4223
                     /gene="ureE"
                     /locus_tag="O0305_002025"
     CDS             3771..4223
                     /gene="ureE"
                     /locus_tag="O0305_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCY0275155.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            4216..4905
                     /locus_tag="O0305_002026"
     CDS             4216..4905
                     /locus_tag="O0305_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCY0275156.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            4918..5532
                     /gene="ureG"
                     /locus_tag="O0305_002027"
     CDS             4918..5532
                     /gene="ureG"
                     /locus_tag="O0305_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCY0275157.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            5532..6368
                     /locus_tag="O0305_002028"
     CDS             5532..6368
                     /locus_tag="O0305_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCY0275158.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(6557..6904)
                     /gene="sarR"
                     /locus_tag="O0305_002029"
     CDS             complement(6557..6904)
                     /gene="sarR"
                     /locus_tag="O0305_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCY0275159.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT         2417 a         1119 c         1218 g         2217 t
ORIGIN      
        1 aaaataaaaa ggaacgtgct gtgaacaatt aaaagaaaac tgataattcg attaagtatg
       61 cacttaattt tatccataaa aatacacctt aaactataca attcgctgta tagtttaagg
      121 tgatattcaa acaatattag gtttgattta actatcaagc atctactttg tcatgtttaa
      181 taccagcaaa taataaaatc catgtcacga taataaatgg caatgttaat gcaggtaagc
      241 caaacggttc aagcaatgtt gttgtaccta gttgcaccac tactgttaat aacacaccta
      301 aaaaagtcga aatataaggg ttaatcgctg ttttaaatgt atatcctagt gcgattgcca
      361 ttaatacaaa gttataaccg aataatccct gattaatatc atcataatta ccacctaata
      421 gagctacaat gataaagctt aacaaactag ctataacagc taagagtgtt gcttttcttg
      481 atgctatcaa tatgccgatt aaaatacata cgccaccaat tacactcgct tcgataaata
      541 cttgactgaa tccttcaaat aatgactgaa tgaaatgtat tctatcattg ttgctaaaat
      601 taaccgtttc aatgttttga ggcattaact ttaacgatgt atctacaaat ttaacctgtc
      661 ctgaaagtaa aattgtaaac caagtcacaa tgacaaaagg catcgtcagc atcggaactt
      721 tatatggtct taaaacttct ctcactgcag cagcaactgg taacgttaat aaagttgcta
      781 tcattgttat aacaatatct aatcctgact tatctaaaaa gattgttaac gcaatggcag
      841 ttagcactgg attaaatcca gctaacccat cattaatctc tgcctcacta taatttataa
      901 aacgcgcaaa agtataggcg atgatgctac ctacaatagc cgctaatcca attgtccaat
      961 cggctacaaa taatcctatt aagataaata atcctgtcca tttattacta attaacacaa
     1021 cctgagatat atttttcaaa agcacatcta ttatcttcaa atctttcacc tttcctttga
     1081 atcattactc attaatatac tactattcta tgaaaagtta tattaaaaac tattttggtt
     1141 tttacataaa tatattccac tatttgtttt cgattagatt gtttgcttca atatcggata
     1201 tgctaaaaat ataattttct aattacacat tgaactttgt atattacttg tttatcacaa
     1261 caattaggat taatataaat tttgtaaatt gtatctaata tgaaattggg ggttttaatt
     1321 tgcattttac acaacgagag caagacaaat taatgattgt agtggcggcg gaagttgcac
     1381 gtcgtcgtaa agcacgtggt ttgaaactaa atcatcctga ggcattagct ttaatcagcg
     1441 atgaattatt agaaggtgca cgcgatggta agaccgttgc agagttaatg agttatggta
     1501 gacaaattct aaacaaagaa gatgtcatgg atggtgtcga acacatgatt acagatatcg
     1561 aaatcgaggc tacgttcccc gatggtacta agttaatcac agtacatcac cctattgttt
     1621 aaaggaggca gtcaaatgat accaggagaa attattacaa aaagtacaga ggttgaaatt
     1681 aataaccatc atcctgaaac agttatcgaa gttgaaaata caggagatcg accaattcaa
     1741 gtgggctcac attttcattt ttatgaagca aatgcagcat tagatttcga acgtgaaatg
     1801 gcatatggaa aacatttaga tattccagct ggagcaactg ttcgatttga acctggggat
     1861 aaaaaagaag ttcaattagt tgaatatgct ggcaaacgta aaatttttgg ttttcgtggt
     1921 atggtcaatg gtcctatcga tgagtcacgt gtctatcgcc caactgatga aaatgatgaa
     1981 tatgcaggtg tattcggaga taacggtgct gaaaacgtga ataaaaaagg aggaaaaaga
     2041 tcatgagctt taaaatgacg caaaatcaat atacgagctt atacggtcca actgttggag
     2101 attccattcg tttaggtgat acgaatctat ttgctcaaat agaaaaagac tatgcggttt
     2161 atggtgaaga agctactttt ggtggtggta aatctattag agacggtatg gcgcaaaatc
     2221 ctcgtgtaac acgtgatgac gtgaacgttg cagaccttgt catttctaat gccgttatta
     2281 tcgattacga taaagtggtt aaagctgata taggcattaa aaatggttat attttcgcca
     2341 taggtaatgc cggcaaccca gatataatgg ataatgtcga cattattata ggttcaacaa
     2401 cagatatcat tgccgctgaa ggtaaaatcg tcactgctgg tggtattgat actcatgttc
     2461 attttattaa tcctgaacaa gcagaggtcg cattagaaag tggtattacg actcatattg
     2521 gtggtggtac tggtgcttca gaaggttcta aagcaacaac tgtaactcca ggtccatggc
     2581 atattcatag aatgttagaa gctgccgaag gtttaccgat taatgtcggt tttacaggta
     2641 aaggacaagc aacaaatcca actgcactca ttgaacaaat caatgccgga gcaattggat
     2701 taaaagtaca tgaagactgg ggtgcaacac catctgcttt gagtcatgca ttagatgttg
     2761 ctgatgaatt tgatgttcaa attgcattac atgcagatac tttaaatgaa gcaggattta
     2821 tggaagacac aatggctgct gttaaagacc gtgtacttca tatgtaccat actgaaggtg
     2881 ctggtggcgg tcatgcgcct gatttaatta aatccgctgc attttcaaat attttacctt
     2941 catctacaaa tccaactttg ccttatacac ataatactgt agatgaacat ttagatatgg
     3001 taatgattac tcaccattta aatgcggcta ttcctgaaga tatcgcattc gcagattcac
     3061 gtattcgtaa agaaacgatt gcagcagaag atgttctgca agatatgggt gtattcagta
     3121 tgattagttc cgattcacaa gcaatgggcc gtgtaggtga agtaattaca cgaacatggc
     3181 aagtagcaca tcgcatgaaa gaacaacgtg gtcctttaga tggtgatttt gaacataatg
     3241 ataataatcg catcaaacgt tatatcgcta aatatacaat taacccagca attacacatg
     3301 gtatttctga atatgtagga tctatcgagc cgggcaaact agctgacatt gtcttatggg
     3361 acccaatttt ctttggggtt aaacctgaat tagttgtaaa gggcggatta attaactctg
     3421 ccgtaaatgg cgatgcaaat ggttctatac ctacatctga accgatgaag taccgtaaaa
     3481 tgtatggtca atacggcgga aaccttacaa gtacgtcaat gacattcgtg tctaaaactg
     3541 cttatgaaaa tggtatcaat cgtgcattaa atttaaaacg catggtgcgt ccagttaaaa
     3601 atattagaca attatctaaa gcagatatga aaaataacag tgcaacacct aaattagacg
     3661 ttgatccaca aacatatgaa gtatatgtag atggagaaaa aattacaagt aatgcagcaa
     3721 ctgagttacc attaactcaa agatacttct tattctagga ggaacataga atgattgttg
     3781 aagaaatcca aggcaatatt gccaatttgt caaattcaga aaaacaaaag cacgtcgaaa
     3841 aagtatacct tgaaaattca gatcttgtta aacgtattca aagagtcgtt acagaccatg
     3901 gcactgaaat aggcattcgt ttaaaacaac ctattgactt acaatatgga gatattttat
     3961 acgcagatga ccataatatg attattgtgg atgttaattc agaagatctt ttagtgattc
     4021 aaccaagaac attgcaagaa atgggagata tagcccatca attaggaaat cgtcatttgc
     4081 cagcacaatt tacagagact gaaatgctag tacaatatga ttatttagtt gaagatttat
     4141 taaaaagttt aggcatccca tatgttcgag aagaccgtaa agttaataag gctttcagac
     4201 atatagggca ttctcatgat tgatcataca cacttaagat tatttcagtt ctgtgattca
     4261 cagtttccaa caggtgcttt cagtcattca tttggtcttg aaacatatat tcaacgaaat
     4321 ataattcatg acgatcatac ttttattgct tggttaaaaa tgtttttaca agagcaactt
     4381 acctattctg atggtttagc catgcgttta gtttatgatg cattggaaaa tgatgataca
     4441 caaaaagtat tacacataga taaactgatg tttgttcaaa acttacctaa agaaacacgt
     4501 gttggtgcaa aacaaatggg gactcgcatg gttaaattgg ctttagaact ttataatagc
     4561 ccatggattg cttggtatca tcaacaaatg caagataaga aagcaaagtt aaatccagct
     4621 atttgtttta ctatgctagg ccatcattta ggtgtagata ttgaaacgat tattgattac
     4681 tatttatatc aaaatgtttc aagtttaact caaaatgctg ttcgtgctat tccacttgga
     4741 caaactgctg gtcaaaagat agttactcac atgattcctt atatagaagg aacaagaaag
     4801 cagattttcg aattaaaaga agcagatttt ggcatgacag cgcctggttt agaactaaat
     4861 caaatggcgc atgagaacgt caatgttaga attttcatat cataggaggt tataattgtg
     4921 gcaaatccga ttaaaattgg tattggtggt cctgtaggtg caggtaaaac acaattaatt
     4981 gaaaaagttg taaaacgtct ttcaaaagaa atgagtatcg gcgttattac aaatgatata
     5041 tatacaaaag aagacgaaaa gatattagta aattcaggag ttctacctga aagtcgtatc
     5101 attggtgttg aaactggtgg atgtcctcat actgcgattc gtgaagatgc atctatgaac
     5161 tttgcagcaa tagacgaatt attagaacgt catgacgata tagaactaat tttcatagaa
     5221 tctggtggcg ataacttagc agcaacattt agtccagaac ttgttgactt ttcaatatat
     5281 attatcgatg ttgctcaagg tgaaaagatt ccacgtaaag gtggtcaagg tatgattaag
     5341 tcagatttct ttgtaattaa caaaactgat ttagctccct atgtaggtgc atcattagaa
     5401 caaatggctg aagatactaa agtatttcgt ggtaaacgtc catttacttt tactaactta
     5461 aaaaccgacg aaggtttgga tgaagttatc gattggattg aacgcgacac tttactcaaa
     5521 ggattatcat aatggatgaa caacaatgga ctgggcaact tgatttaaca gtgtttttcg
     5581 atggcaatcg atcagtatca agagatattt tctttgaaaa agcacttaaa gtgatacgtc
     5641 cagtttatct aaatcaatct accattccta cattttatat agtaaatgta ggtggtggct
     5701 atttagatgg agatcgttac cgtatgaatg tgaatgtcga agataacgct aaagtgacat
     5761 tgacatctca aggtgcaaca aaaatataca agacaccttc caatcatgtt gagcagtatc
     5821 aaacttttaa tttgaaagat aacgcatatt tagaatatgt cgctgatcca atcatcgcat
     5881 atgaaaatgc taaattttat caacacaata cgttcaatct caataattct agttcattat
     5941 tttatactga tattttaact cctggttatt caaaaactgg cgaagccttc aagtatcaat
     6001 atatgcattt aataaatgaa atttatattg aagatgagtt agtcacatat gataatttat
     6061 tattgaatcc taataaacaa tcgatcaatg aaataggtta tatggaacat tactctcatt
     6121 atggctctgc ttacttcata cacgaagatg ttaaccaaaa actaatcgat tcagtttatg
     6181 aaaccattag ttcttatagc aatacattcg attgtcgtgt tgctatttct caattgccta
     6241 cacatggttt tgcagttaga atttttgctt atcgcacaca aattatagag aaaatacttg
     6301 gaaccatcca aagctatatc gcagaaaata tttatgatcg taaacttgat tttctaagaa
     6361 aatattaaaa taaaaagact agtgtacctt gtttcaagct taataagctg aaataaggta
     6421 tggctagtct ttaactttaa ttaaagcatc ttcaatattt aaattttaag ttaattttat
     6481 gtcccaatgc tgaataaata acttcgtttt aataaattgt tatcgttcaa tgttattaaa
     6541 cgcaattaac cttgatttaa tttttaatgt attcttctaa ttctgaaatc agtttttgaa
     6601 tatttgcttt ttgtgtatct gtaacataaa caataactgt tctttcgtct tgtaaacttc
     6661 ttttctttga taacaatttt aaatctttta gcttttgtaa agctttagtt aaatagtaag
     6721 gtttgaactc tgagcactta gcaatctctt tagatgagat ttcgttagac tcacttctta
     6781 aaatatgatt taaaatataa atttcttcat agttcaaatt gaacttcttt tttgtatctc
     6841 tgaaaaactt cttaacttga aatgttgcgt tgactaaatc attaatgtca ttaattttac
     6901 tcattattaa accactcctc tgatgcacat cttgttgtag caaggttcac atgtaattta
     6961 ttaaatcgaa a
//
LOCUS       DAKTIP010000137         6752 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.138, whole genome shotgun sequence.
ACCESSION   DAKTIP010000137 DAKTIP010000000
VERSION     DAKTIP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.138"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            69..1052
                     /locus_tag="O0305_002030"
     CDS             69..1052
                     /locus_tag="O0305_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCY0275160.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1243..2169
                     /gene="pstC"
                     /locus_tag="O0305_002031"
     CDS             1243..2169
                     /gene="pstC"
                     /locus_tag="O0305_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCY0275161.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2171..3088
                     /gene="pstA"
                     /locus_tag="O0305_002032"
     CDS             2171..3088
                     /gene="pstA"
                     /locus_tag="O0305_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCY0275162.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3135..3986
                     /locus_tag="O0305_002033"
     CDS             3135..3986
                     /locus_tag="O0305_002033"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCY0275163.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            3993..4634
                     /gene="phoU"
                     /locus_tag="O0305_002034"
     CDS             3993..4634
                     /gene="phoU"
                     /locus_tag="O0305_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCY0275164.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4774..6588)
                     /gene="pepF"
                     /locus_tag="O0305_002035"
     CDS             complement(4774..6588)
                     /gene="pepF"
                     /locus_tag="O0305_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0275165.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         2351 a         1065 c         1154 g         2182 t
ORIGIN      
        1 ttcaaggtta agataaagat gtaatcaata caaatactat ttgttgttca tacagggagg
       61 atatttcaat gaaaaaatgg caatttgttg gtactacagc tttaggtgca acactattat
      121 taggtgcttg tggtggcggt aatggtggca gtggtaatag tgatttaaaa ggggaagcta
      181 aaggtgatgg ctcatcaaca gtagcaccaa ttgtggagaa attaaatgaa aaatgggctc
      241 aagatcactc ggatgctaaa atctcagcag gacaagctgg tacaggtgct ggtttccaaa
      301 aattcattgc aggagatatc gacttcgctg atgcttctag accaattaaa gatgaagaga
      361 agcaaaaatt acaagataag aatatcaaat acaaagaatt caaaattgcg caagatggtg
      421 taacggttgc tgtaaataaa gaaaatgatt ttgtagatga attagacaaa cagcaattaa
      481 aagcaattta ttctggaaaa gctaaaacat ggaaagatgt taatagtaaa tggccagata
      541 aaaaaataaa tgctgtatca ccaaactcaa gtcatggtac ttatgacttc tttgaaaatg
      601 aagtaatgaa taaagaagat attaaagcag aaaaaaatgc tgatacaaat gctatcgttt
      661 cttctgtaac gaaaaacaaa gagggaatcg gatactttgg atataacttc tacgtacaaa
      721 ataaagataa attaaaagaa gttaaaatca aagatgaaaa tggtaaagca acagagccta
      781 cgaaaaaaac aattcaagat aactcttatg cattaagtag accattattc atttatgtaa
      841 atgaaaaagc attgaaagat aataaagtaa tgtcagaatt tatcaaattc gtcttagaag
      901 ataaaggtaa agcagctgaa gaagctggat atgtagcagc accagagaaa acatacaaat
      961 cacaattaga tgatttaaaa gcatttattg ataaaaatca aaaatcagac gacaagaaat
     1021 ctgatgataa aaagtctgaa gacaaaaaat aataagacgc aatttcaaat gtgtcttgaa
     1081 acatgatttt gatggtgaat cattatttag agtacaaagc ttgatttatc gagacgctga
     1141 ttttgacatt cagttagtct acaagcttat caacttaaaa tagtggttca tcattatttt
     1201 acaaatctaa ttattttggg agtaatagaa agaggtttga ttatgacttc atctactaat
     1261 gttaaagctt taatcgaaaa aaataataat aaaaaaggaa agcataatga caaaattata
     1321 ccagttattt tagccgcaat ttcagcgatt tccattttaa caacactagg tatattaatc
     1381 acattgcttt tagaaaccat cacttttttc accagaattc caataactga atttctattt
     1441 tctactactt ggaatcctac cggttcagac cctaagtttg gtatctgggc attgataata
     1501 gggactttaa aaatcacagt tattgcgact atatttgcag ttccagtcgg gcttggtgca
     1561 gcaatttatt taagtgaata tgcaagtgac cgtgcacgac gcataattaa accgatatta
     1621 gaaattttag caggaatacc aacaattgtg tttggtttct ttgcattaac ctttgttaca
     1681 ccagtattaa gatctttcat accaggtctt ggagagttta atgctataag tcccggctta
     1741 gttgtcggta ttatgattgt ccctctcatc acaagtttga gtgaggatgc aatggcatct
     1801 gtaccaaata aaattcgaga aggtgcctat ggacttggag caactaaatt agaagtagca
     1861 actaaagtcg tacttcccgc agcaacatca ggtattgtag cttcaatcgt tctcgcgatt
     1921 tcaagagcaa ttggagaaac gatgattgta tcattagcgg caggtagttc gccaacagct
     1981 tcattaagtt taacaagttc gattcaaaca atgactggat atattgttga gatagcgaca
     2041 ggtgatgcaa catttggatc aaatatttat tacagtattt atgctgtagg gttcacacta
     2101 tttatcttta ccttaatcat gaatttactt tctcagtgga tttctaagcg ttttagggag
     2161 gagtattaat atggaaacga cagataataa tagacaatca ctcgtcgatc aacaacttgt
     2221 ccaaaaacat ttatcatcca gaacggttaa aaataaagtg ttcaaactca tatttttagc
     2281 atgtacatta ttaggacttg tcgtacttat tgcgttgtta actcaaacat tgattaaagg
     2341 ggtaagtcat ttaaatttac agtttttcac taatttttct tcttcaacac catctatggc
     2401 tggcgttaaa ggcgcgttaa tcggttcact ttggttaatg ttaagtatca ttccattatc
     2461 aatcatccta ggaataggta cagctatata cttagaagaa tatgcgaaaa acaacaaatt
     2521 tactcagttt gttaaaatca gtatttccaa tttagctggt gtaccatcag ttgtatttgg
     2581 gttattaggt tatactttgt tcgttggtgg tgcagggatt gaagccttga aaatgggtaa
     2641 cagtatattg gcagcagcgc taacaatgac cttactgata ttaccaatta ttattgtttc
     2701 aagtcaggaa gcaattagag ctgtacctaa ctcagtacgc gaagcttctt acggcttagg
     2761 tgctaataaa tggcaaacga taagacgtgt tgtcttacca gcagcgttac ctggtatttt
     2821 aactggattc attttgtctc tttcaagagc actgggagaa acagcgccac ttgtgctaat
     2881 cggtataccg actatattat tggcaacacc tagaagtata ttggatcaat tttcagcatt
     2941 acctatccaa atatttactt gggcgaaaat gcctcaagaa gaattccaga atgttgcatc
     3001 ggcaggcatt atcgttttac tagttatctt aatcttaatg aatggcgttg cgattatttt
     3061 acgtaacaaa tttagtaaaa aattctaatt taaacaatca atctcattta tctattaaaa
     3121 agggagtttt aaatatggcg caaacacttg cacaaactaa acaaatatct caaagtcata
     3181 cgtttgatgt ctcacaaagt catcataaaa caccagatga tacaaactca cattctgtta
     3241 tatattcaac acaaaattta gacttatggt atggcgaaaa tcatgcatta caaaatatta
     3301 atttagatat ttatgaaaac caaattactg ccattatagg tccatctggt tgtggcaaat
     3361 caacatatat taaaactttg aatcgaatgg ttgagttagt accttctgtg aaaacagctg
     3421 gtaaaatatt atatcgagat caagacattt ttgatcaaaa atattctaaa gaacaattac
     3481 gtacaaatgt gggcatggtc tttcaacaac ctaatccatt tccaaaatca atatacgata
     3541 atattactta cggtccaaag attcacggta ttaaaaataa aaaagttctt gatgaaatcg
     3601 ttgagaaatc attacgtggc gctgcaattt gggatgaatt aaaggatagg ttgcacacaa
     3661 atgcatatag tttatccggt gggcaacaac aacgtgtttg tatcgcgcgt tgtttagcaa
     3721 ttgaacctga agtcatttta atggatgaac cgacatcagc attagatcca atctcaacat
     3781 taagagtaga agagttggtt caagaactaa aagaaaagta tacaattatt atggttacac
     3841 ataatatgca acaagcagct cgtgtatcag ataaaactgc atttttctta aatggttatg
     3901 tcaatgaata tgatgatact gataaaattt tctctaaccc atcaaacaag aaaacagaag
     3961 attatatttc aggaaggttt ggttgatata taatggcaat aattagacaa cgatatcagg
     4021 agcaacttga tgatttaata aaagaattac gtcggttagg tgcaaatgtc tatgtgagta
     4081 ttgaaaatgg tataaaatca ttaagtattg acgatagagg ctttgcacga caaacagtta
     4141 aaaacgataa acatatcaat caattaaatt atgatattaa tgagcgagtt atcatgttaa
     4201 ttacaaagca acagcccatt gcgagtgatt tgcgtatgat gatttcttca ttaaaaatcg
     4261 cctccgattt agaaagaata ggagataatg cctcgagtat tgccaatatt cgattgcgta
     4321 caaagattac agatgattat gtgttaaccc gtttaaagac aatgggtaaa ttagctatgt
     4381 taatgttaaa ggacttagat caagcattta aaaagaaaga taccgtatta ataagagaaa
     4441 taattgagcg tgatgaagat atcgatgact tatatagtca tattattaac gcaacgtatc
     4501 ttattgataa cgatccattt gtcgctgcac aagctcattt agcagcaaga catttagaac
     4561 gtattggtga tcatattatt aacatcgctg aaagtgttta tttttattta acaggtacac
     4621 attacgaaca ataacttaaa gttattacta taaaatccct tacgataaat atatatttct
     4681 attattcata aaccctcaaa aaaaccaaga ttctcacaat tagtaatgtg aaaatcttgg
     4741 tttatattgt tctactataa attgtctcgc atcttagtta tttgcttgct caatttcatc
     4801 tgttaatttt tcaacttcat cgactaaatc agaaatatat tgaattgtag atttaagtgg
     4861 ctgttctgta gtaatgtcta cacctgcaat gtttgcaagt tcgacaggtg atacactacc
     4921 accttttttc aatgtttcta accaagcatc aacagctggt tggccttcat ttttaatctt
     4981 ttgagaaacg acagttccga ttgttaagcc agcagaatac gtatacgaat ataatcccat
     5041 atagtaatga ggttgacgca tccatgttaa ttcagcaccc tcagtcatgt ctactgcatc
     5101 tccaaaaaat tgtttataaa catttagcat tatttcattt aatgtcggcg catttaaaga
     5161 ttcaccttga tctactttgt gatacacttc acgttgataa gcagcttcta ataaatgggt
     5221 aaccatatta tgataatatg ttctagataa aattgagcca ataacccaac gcttaaatct
     5281 tggattatca cttgtgttaa ataaataatt ggccatcaac atttcattca ttgtagaagg
     5341 ggcttcaaca aagtacattg atgcttctga ttcaagatat ggttgatgtt tttgagctaa
     5401 tgtaaaatga cctgcatgac ctaattcatg tgctaagaca aatgcttcag ccattttacc
     5461 agtccaagaa ataaacacat atgaatgtgt aaagtatgga cttgcacaaa atgcgcctgt
     5521 atctttacct ttattttgtg caaaatcaat ccatcgctga tcgtatgctt cacgtaacat
     5581 gtttgtatag tcatcaccta aaacacttaa cgcaccgaaa atataatttt ttgagtcttc
     5641 aattgaaatc tctggttcat aatcagggtc tacagaaatc ttcaagtctt caaaacgcat
     5701 gttatctaat ccgtgaatac gttgtaaaag tttagcatat ttctgcataa ctggtgccaa
     5761 gtcacgcata atcatatcga tttgacggtc aaacatatca cgcgttactt cttgactatg
     5821 taataaataa tcgatgactg attcaaatcc acgtaaatca gcttcaattt tttcttgttg
     5881 tacttgcata ttatatgtag ccgcggtagt atgctgatat tttcgaatcc catcgctaaa
     5941 cgatttgaaa cttttacgtc taaactcagg atctttatta tcttcataat cattttcaaa
     6001 cgtagcatag tcgacagggt acgttgtacc atcatgttca aatgaatcga atgtaatatc
     6061 tagcattttt gtcgtgccgt ataaatcgta aggactgttt agcgtaggtg ataaagttgc
     6121 taatacttgt tctaccgacg cagataattg gaatggcttt tgttttatta actgtttaat
     6181 atagtgttga tatggacatg attcttctaa ttgttgaagt atttcttctg gtaattcaag
     6241 tatttctgac tctacaaatg ataattggct aacaatttta ccgtatgtag tagataattt
     6301 agcgctcaat acttgtgcct cgatattact agtatctaca cttaaacgta gttctgcata
     6361 attacttaag cgatctaagg caattaaaat attttctaat tcagctaaag cagtattaat
     6421 ttgttcgatt gaatttaatg ttgttgcata tgtatgatga aattgatttg cttgttgtac
     6481 tagagcgtca atactttcat aatattgttg atcatcttta aataagtcta ctaaatccca
     6541 tgtttctgaa acaggaacat cttctcttaa aggtaaacct tgagacatat aatcactcct
     6601 ttggtataat acattcatta tacataattt tgtctcctaa acgataatgt tacgatttcc
     6661 atgtcgcaag gaacaatgat ttttctatta gtagatagaa tattgcttga aactgactgc
     6721 tattgtttaa gtttttaaaa atttataata aa
//
LOCUS       DAKTIP010000138         6749 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAKTIP010000138 DAKTIP010000000
VERSION     DAKTIP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.139"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            202..1431
                     /locus_tag="O0305_002036"
     CDS             202..1431
                     /locus_tag="O0305_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCY0275166.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTLAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1626..2483
                     /locus_tag="O0305_002037"
     CDS             1626..2483
                     /locus_tag="O0305_002037"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCY0275167.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2483..3427
                     /locus_tag="O0305_002038"
     CDS             2483..3427
                     /locus_tag="O0305_002038"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCY0275168.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3696..4664
                     /gene="pfkA"
                     /locus_tag="O0305_002039"
     CDS             3696..4664
                     /gene="pfkA"
                     /locus_tag="O0305_002039"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCY0275169.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4686..6443
                     /gene="pyk"
                     /locus_tag="O0305_002040"
     CDS             4686..6443
                     /gene="pyk"
                     /locus_tag="O0305_002040"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCY0275170.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2389 a          899 c         1368 g         2093 t
ORIGIN      
        1 ttaaaggtat atctttagat agtatggttt aatgtatctt tataaatatt attttattat
       61 cgtttatcgc tatttgtgtt acataatcaa aaccataaat tcttactcat tcagatttac
      121 ccaatatttt tacttttata atgtaatgcg ttttatccaa gttatttttt aaaaataaat
      181 attgaattag gggctgattt catgtcatta agagatgaag cattggaaat gcacaaacgt
      241 aatcaaggta aattagaagt taaaccaaat gtaaaagtta ctaataaaga ggaattaagt
      301 ttagcatact cacctggcgt tgctgaaccg tgtaaagata tttatgaaga taaaagaaaa
      361 gtatatgatt acacaattaa aggaaataca gttgcagtta ttactgatgg aacagcggta
      421 ttaggtttag gtaacattgg acctgaagca agtattcctg taatggaagg taaagcagta
      481 ttattcaaaa gcttcgctgg tatcaatggg gtgcctattg cgttaaatac aactgatacc
      541 gaagaaatca ttaaaacagt taagttgtta gaacctaatt atggtggtat taatttagag
      601 gatatttcgg caccacgttg ttttgaaatt gaagaacgat tgaaaaaaga aactaatatt
      661 ccggtattcc atgacgatca acatggtaca gcaattgtaa cattggcagg tttggtaaat
      721 gcattgagag ttgttaacaa agatattgct aaaataaaag ttgtactaaa tggtgctggt
      781 gcagcaggaa tagccattgt taaattacta tacgcgtatg gtgtaagaaa tatggttatg
      841 tgtgactcaa gaggcgcaat ttttgaagga cgttcatatg gtatgaatcc tacgaaagat
      901 gttgtagcaa aatggacaaa taaagataag attgaagggt ctttagaaga agtcgtaaaa
      961 gatgcagatg tatttatcgg ggtttctgta gctaatgcgc tgtcacaaga tatggttaag
     1021 agtatggcag ataatccaat tatatttgca atggctaatc caaatcctga aataatacct
     1081 gatgatgcca aagcggcagg tgcacgagtt gttggtacag gacgttcaga ctatcctaac
     1141 caaattaata atgtattagc tttccctggt atttttagag gtgcattaga ggttgaagct
     1201 acacatataa atgaagaaat gaaaaaggca gctgtagaag cgattgctga tttaatcgat
     1261 agttctgaat taaatgaaga ctactgtatc ccaggaccgt ttgataaacg tgtagcgcca
     1321 tcagttgctc gtaatgttgc taaagcggca atggaatctg gagtagctag gattgaagtt
     1381 gatccgcaag atgtgtatga taaaacaatg aaacttacag atttacaata aaaataatta
     1441 acgtttaatt aaattgctta aaagttagca ataaagagtc attaaaatga tgaaaatgaa
     1501 agtttatgat aaacattcaa cagtcaaacg aatataaatc aaataaattt aaacccgttt
     1561 ttaactggtc aagttcagtt taaggctcta aatggttaga acagaggtta tttggaggtt
     1621 ttcctatgtt taaagatttt tttaatcgaa caaagaaaaa gaaatatctt acagtacaag
     1681 actctaaaaa taatgatgtg cctgcaggta ttatgactaa gtgtccaaag tgtaagaaaa
     1741 ttatgtacac aaaagaatta gctgaaaatt taaatgtgtg ctttaattgt gatcatcata
     1801 ttgctttaac tgcgtataaa cgtatagaag caatttctga tgaaggatca tttacagaat
     1861 tcgataaggg aatgacctct gcgaatccat tagattttcc aagttattta gaaaaaattg
     1921 aaaaggacca acaaaagaca ggtcttaaag aagcagttgt gactggtaca gcacaactag
     1981 atggtatgaa atttggcgtt gctgtcatgg attcacgttt tagaatggga agtatgggat
     2041 cggttatcgg tgaaaagata tgtcgcatca ttgattactg cactgagaac cgtttaccat
     2101 ttattctttt ctctgcaagt ggtggtgcac gtatgcaaga aggtattatt tccttgatgc
     2161 aaatgggtaa aaccagtgta tctttaaaac gtcattctga cgctggacta ttatatatat
     2221 catatttaac acatccaact actggtggtg tatctgcaag ttttgcatca gttggtgata
     2281 taaatttaag tgagccaaaa gcgttgatag gttttgcagg tcgtcgagtt attgaacaga
     2341 caataaacga aaaattgcca gatgatttcc aaactgcaga atttttatta gagcatggac
     2401 aattggataa agttgtacat cgtaatgata tgcgtcaaac attgtctgaa attctaaaaa
     2461 tccatcaaga ggtgactaaa taatgttaga ttttgaaaaa ccactttttg aaattcgaaa
     2521 taaaattgaa tctttaaaag aatctcaaga taaaaatgat gtggatttac aagaagaaat
     2581 tgacatgctt gaagcgtcat tggaacgaga aactaaaaaa atatatacaa atctaaaacc
     2641 atgggatcgt gtgcaaattg cgcgtttgca agaaagacct acgaccctag attatattcc
     2701 atatatcttt gattcgttta tggaactaca tggtgatcgt aattttagag atgatccagc
     2761 aatgattggt ggtattggct ttttaaatgg tcgtgctgtt acagttattg gacaacaacg
     2821 tggaaaagat acaaaagata atatttatcg aaattttggt atggcgcatc cagaaggtta
     2881 tcgaaaagca ttacgtttaa tgaaacaagc tgaaaaattc aatcgtccta tctttacatt
     2941 tatagataca aaaggtgcat atcctggtaa agctgctgaa gaacgtggac aaagtgaatc
     3001 tatcgcaaca aatttgattg agatggcttc attaaaagta ccagttattg cgattgtcat
     3061 tggtgaaggt ggcagtggag gtgctctagg tattggtatt gccaataaag tattgatgtt
     3121 agagaatagt acttactctg ttatatctcc tgaaggtgca gcggcattat tatggaaaga
     3181 cagtaatttg gctaaaattg cagctgaaac aatgaaaatt actgcccatg atattaagca
     3241 attaggtatt atagatgatg tcatttctga accacttggc ggtgcacata aagatattga
     3301 acagcaagct ttagctatta aatcagcgtt tgttgcacag ttagattcac ttgagtcatt
     3361 atcacgtgat gaaattgcta atgatcgctt tgaaaaattc agaaatatcg gttcttatat
     3421 agaataatca acttgagcat ttttatgtta aatcgatact gggttttacc ataaattgaa
     3481 gtacattaaa acaataattt aatatttaga tactgaattt taactaagat tagtagtcaa
     3541 aattgtggct actaatcttt ttttaattaa gttaaaataa aattcaatat ttaaaacgtt
     3601 tacatcaatt caatacatta gttttgatgg aatgacatat caatttgtgg taatttagag
     3661 ttaaagataa atcagttata gaaaggtatg tcgtcatgaa gaaaattgca gttttaacta
     3721 gtggtggaga ttcacctgga atgaatgctg ccgtaagagc agttgttcgt acagcaattt
     3781 acaatgaaat tgaagtttat ggtgtgtatc atggttacca aggattgtta aatgatgata
     3841 ttcataaact tgaattagga tcagttgggg atacgattca gcgtggaggt acattcttgt
     3901 attcagcaag atgtccagag tttaaggagc aagaagtacg taaagttgca atcgaaaact
     3961 tacgtaaaag agggattgag ggccttgtag ttattggtgg tgacggtagt tatcgcggtg
     4021 cacaacgcat cagtgaggaa tgtaaagaaa ttcaaactat cggtattcct ggtacgattg
     4081 acaatgatat caatggtact gattttacaa ttggatttga cacagcatta aatacgatta
     4141 ttggcttagt cgacaaaatt agagatactg cgtcaagtca cgcacgaaca tttatcattg
     4201 aagcaatggg ccgtgattgt ggagatctag cattatgggc tggattatca gttggtgctg
     4261 agacaattgt agttccagaa gtgaaaacag atattaaaga aatagctgat aaaattgaac
     4321 aaggtattaa acgtggtaag aaacactcaa tcgttcttgt agcagaaggt tgtatgactg
     4381 cgcaagattg tcaaaaagaa ttatcacaat acatcaatgt tgataataga gtgtctgtgt
     4441 taggtcacgt tcaacgtggt ggtagcccaa caggtgcgga tagagtttta gcatcacgtt
     4501 taggtggata tgcggtagac ttattaatgc aaggtgaaac agctaagggt gttggaatta
     4561 agaacaataa aattgtagca acatcttttg atgaaatttt tgatggtaaa gatcataaat
     4621 ttgattatag tctatatgaa cttgctaaca agttatctat ataagatttc aggaggaatt
     4681 ataaaatgag aaaaactaaa attgtatgta caattggacc agcttcagaa tcagaagaaa
     4741 tgattgagaa attaatcaat gctggtatga acgttgcacg attaaacttt tcacatggta
     4801 gtcatgaaga gcataaaggt agaattgata caattcgtaa agtagctaaa agattagaca
     4861 aaattgtagc aattttatta gatacaaaag gtccagaaat tcgtacgcat aatatgaaag
     4921 acggtatcat tgaacttgaa cgtggcaacg aagttattgt tagcatgaat gaagttgaag
     4981 gaacacctga aaagttctca gtaacatatg aaaacttaat taacgatgtt caagtaggtt
     5041 catacatttt acttgatgat ggcttaattg aattacaagt taaagatatt gaccatgcta
     5101 aaaaagaagt taaatgtgat attttaaact ctggtgagct taaaaacaaa aaaggtgtta
     5161 acttacctgg cgtaagagta agtttacctg gtattacaga aaaagatgct gaagatatcc
     5221 gtttcggtat taaagaaaat gttgacttca ttgcagcaag tttcgtacgt cgtcctagtg
     5281 atgttttaga aattcgtgaa attttagaag aacaaaaagc taacatttca gtattcccta
     5341 aaattgaaaa ccaagaaggt attgataata ttgcggaaat tcttgaagtg tctgatggtt
     5401 taatggttgc acgtggtgac atgggtgttg aaattccacc tgaaaaagta ccaatggttc
     5461 aaaaagattt aatcagacaa tgtaacaaat taggtaaacc agttattaca gctacacaaa
     5521 tgttagattc tatgcaacgt aacccacgtg ctacacgtgc agaagctagt gacgttgcca
     5581 acgcaatcta tgatggtaca gatgcagtaa tgttatctgg tgaaactgct gctggtttat
     5641 atcctgaaga agctgttaaa acaatgagaa atattgctgt atcagctgaa gcagcccaag
     5701 attacaaaaa gttattgtca gatcgtacta aattagttga aacttcatta gtgaatgcta
     5761 tcggtatttc ggttgcacat acagctttaa acttaaatgt taaagcaatt gtagctgcta
     5821 ctgaaagtgg ttcaacggca cgtactatct ccaaatatcg tccacattca gacattattg
     5881 cggtgactcc aagtgaagaa actgcacgtc aatgttcaat tgtttgggga gttcaacctg
     5941 tagttaaaaa aggacgtaag agtacagatg cattgttaaa caatgcagtt gcaacagctg
     6001 ttgaaactgg tagagtatct aatggtgatt taatcattat tactgctggt gtaccaactg
     6061 gtgaaactgg aactactaat atgatgaaaa tccacctagt tggtgacgaa attgctaatg
     6121 gtcaaggtat tggacgtgga tcagttgttg gtactacgtt agttgctgaa actgttaaag
     6181 atttagaagg taaagattta tctgacaaag ttatcgttac taactcaatc gatgaaacgt
     6241 ttgtacctta tgtagaaaaa gctttaggct taattacaga agaaaatggt attacatcac
     6301 caagtgcaat tgttggttta gaaaaaggta ttccaacagt tgtaggtgta gaaaaagctg
     6361 ttaaaaacat aagcaataac atgttagtta cgattgatgc tgctcaaggt aaaatctttg
     6421 aaggatatgc aaacgtacta taatttataa aaaaacgtct ttccatttat caacaatgga
     6481 aaggcgtttt ttggttcatc tggtatttta tgacgtaatt aataggttat ttgataatga
     6541 tagtgtatga atggcaatct atataaatgt ttatatcttt tatacatgta cattatcacc
     6601 ttcaaacttt cactcatatt actttggaaa tttattataa aatagaagta tggatgtatt
     6661 tctgaaatga tacattatta aatagatgag aaagtaaaag ttttgagcca agtacgcaat
     6721 ttaatattat aagttgcata taaaacagg
//
LOCUS       DAKTIP010000139         6741 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAKTIP010000139 DAKTIP010000000
VERSION     DAKTIP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.140"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..567
                     /locus_tag="O0305_002041"
     CDS             <1..567
                     /locus_tag="O0305_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCY0275171.1"
                     /translation="RSFHAISSFKLENLCSLYDIDKEKLHSAKFDVLALSKMLISLAK
                     NNQHYSVLKLIHYMPKQYIRFSKYSNSPTKLFDSGFQKIHMKISEINKIEVESVIPIL
                     KDKNVVFTGNFDTEKQDLMILTRKKGAYIRSDVTAKTDILVEGVQDDKYKDVNGLVSK
                     QRKAREYVGNGAKIQFLNEEDLINLIKE"
     gene            573..914
                     /locus_tag="O0305_002042"
     CDS             573..914
                     /locus_tag="O0305_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275172.1"
                     /translation="MIKKIFTKKHVFLVIEDENHNHSDAVFGKSILLSIYVGVNKKTN
                     SKSGKFIYLDRSKRIVRQSDITKIESANENDVDFYNLLKKEKEIVYSKNIVDKYNLAN
                     YIIYYEVSTKE"
     gene            1118..1300
                     /locus_tag="O0305_002043"
     CDS             1118..1300
                     /locus_tag="O0305_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275173.1"
                     /translation="MKITNCKIKRETVIYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            1378..2091
                     /locus_tag="O0305_002044"
     CDS             1378..2091
                     /locus_tag="O0305_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCY0275174.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMNKNDSNTEDIVNVIPLSSKENKKYLKMN
                     FDLKWEYYLRLFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSLEIEN
                     KLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVIDSSD
                     IMLLINRINNNILQIPDIR"
     gene            2282..3319
                     /locus_tag="O0305_002045"
     CDS             2282..3319
                     /locus_tag="O0305_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0275175.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNISIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            3370..4200
                     /gene="sph"
                     /locus_tag="O0305_002046"
                     /pseudo
     CDS             3370..4200
                     /gene="sph"
                     /locus_tag="O0305_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(4438..5454)
                     /gene="lukG"
                     /locus_tag="O0305_002047"
     CDS             complement(4438..5454)
                     /gene="lukG"
                     /locus_tag="O0305_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCY0275176.1"
                     /translation="MIKQLCKNITICTLALSTTFTVLPATSFAKINSEIKQVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKDKMPVTVSEGFNPEFLAVMSHDKKDKGKSQF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(5476..6531)
                     /gene="lukH"
                     /locus_tag="O0305_002048"
     CDS             complement(5476..6531)
                     /gene="lukH"
                     /locus_tag="O0305_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCY0275177.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNRKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKDGQRLIVTYEVDWKNKT
                     VKVVDKYSDDNKPYKEG"
BASE COUNT         2466 a          974 c          988 g         2313 t
ORIGIN      
        1 agaagcttcc acgcaatctc tagttttaaa ttagaaaatt tgtgtagctt atatgatatc
       61 gataaagaaa aattacattc tgctaaattt gacgtgctag ctttatcgaa gatgttgata
      121 tcactcgcta aaaacaatca gcattatagt gtattaaaat taatacatta tatgcctaag
      181 caatacatta gatttagcaa atattctaac tctccaacta aacttttcga ttcaggattt
      241 caaaaaattc atatgaaaat atctgagatt aataaaatag aagtggaaag tgtaatccct
      301 attttaaaag ataaaaatgt tgtttttaca ggtaattttg acactgaaaa acaagattta
      361 atgatattaa ctagaaagaa aggagcttat atcagaagtg acgtaactgc aaaaacagat
      421 attttagtcg aaggtgttca agatgataaa tataaagatg tgaacggact agtttcaaaa
      481 caacgaaaag ctcgagaata tgttggaaat ggtgcaaaaa ttcaattttt aaatgaagaa
      541 gacttaataa atttaataaa ggaataataa cgatgatcaa aaaaattttt acaaaaaagc
      601 atgtattctt agttatagaa gatgaaaacc ataatcacag tgatgctgtt tttggaaaaa
      661 gtatattact ttcaatttac gtcggtgtga ataaaaagac taattctaaa tcagggaaat
      721 ttatatacct tgacagatct aaaagaatcg ttagacaatc tgatatcacc aaaatagaat
      781 cagctaacga aaatgatgta gatttttata atttactgaa gaaagaaaag gaaattgttt
      841 attccaaaaa tatagtagat aaatacaatt tagcgaacta tataatttac tacgaagtta
      901 gtactaaaga ataaaccaat ccattatttc ataatactaa ccttaaattt acagaggttt
      961 taattatgaa acatgaaaaa agcaatctta actttaagtc ttatatttat tacctactac
     1021 ctcactttta aatatatgtg gattaaagaa ttgaagtatt aattatgctt atttaaaaaa
     1081 gacgtctatt tcagcagtgt ttgaaaggaa gtttataatg aaaataacta attgcaaaat
     1141 aaaaagagaa actgtaatat acgaagtttt aactagtggt aatcaaccat tcacttatga
     1201 gttacctaaa gatttatcgt cacataatgc gcgtaaatac ttggaattta tttcacaaaa
     1261 aatagatggc gataagttaa ccaaagaaga ttcattatga ttttactaat caaaaaacgt
     1321 ctacaagtgt agacgttgaa tggtggtgag agtgtgagcg agaataaagg agaaatgatg
     1381 acgcataata tagaaaaacg cattaataaa ttaaaaactt ctggaaatcc aaaatttaaa
     1441 aaattagatt cagatattca ctatttactc aagagatttg aaggtgaaaa aaaccataaa
     1501 ggtttttatc caaagtttaa acaaggagaa atagtttttg tagatttcgg tataaacgtt
     1561 aataaagaat tctctaattc acactttgca atagtgatga ataaaaatga ttctaatacg
     1621 gaagatatag taaatgttat tcccttatct tctaaagaaa acaaaaagta tttaaagatg
     1681 aattttgatt tgaaatggga gtattattta agattgtttt taaatttaat tagcgcgcaa
     1741 aataattcag ctatattaaa agaagttttc gataaaaaat accaaaaaaa caacacagaa
     1801 ttcatcacta aagattattt tagtgaattt atatctgata gtttagaaat tgaaaataaa
     1861 ttaaataaaa ttgacagaaa cattaataac atagtatcag caattgataa ggtaaaaaaa
     1921 ttaaaaggta atagttacgc ttgcataaat tctttccagc cgattagtaa gtttcgcata
     1981 agaaaagttt taccccaaaa aattaaaaat ccagtaatag attcttcgga tattatgtta
     2041 ctgataaata gaattaataa taatatattg cagattcctg atataagatg atataatttt
     2101 aatatattaa aggtttatcc tttaaaacac gtatatattc gttaccattt ttggtaatta
     2161 accatgtaat cttataacta taagtggcgt ctgtatttta tacaggcgtc tttttttata
     2221 caattttcat gggtagcccg cctaccctta ttattttttg ccaattttga ggagggaacg
     2281 catgaaaaca cgttgttacg atggtaaaaa atggcaatat gaatttaagt atgaaggaaa
     2341 aagataccgt aagaaaggtt ttagaacaaa gcgtgaagct aattctgctg gactagacaa
     2401 gttaaatgag ttaagaagtg gttttaatat agataactat ataactcttg aagaatactt
     2461 cgaaaattgg attaaaacgt ataaacaacc tgttgttaaa gaaaatacct accgtcatta
     2521 tagaaatgca ttacaacata tacaaaaaca taaaataggt aaaatggagt tatcaaagat
     2581 aaatagacaa gtttatcaga aattcataaa cgattattca aaagaacacg caaaagaaac
     2641 tataagaaaa acaaacggtg ctattcggtc agctttagat gacgcattat atgatgggct
     2701 tatttttaaa aatcccgctt ataaagttaa ttataaagcc ggaaaaccta cgaagtcaga
     2761 acaagaaaaa ttcatctcgg taactgaata tgaaatacta aaagatcacg tcagaaagaa
     2821 gagaactcgt tcatcattag cgctattcat aatgatttgt acgggttgtc gtgtcagtgg
     2881 tgcaagaaat ataaagattg agcatatcaa ccaagtgaaa aacactatat ttattgacga
     2941 gcgaaaaacc gatacttccc ctagatatat cagtatcgct aaatctgata tgaaacacat
     3001 tatggacgtc ataagtacat ttgcaattag ctatgatggt tacattttca aagaagccgg
     3061 atctataatt aaccttcatg ctatcaataa tgctttgaaa tcagcctgta gagtcaataa
     3121 tatatcaatt attacatcgc acgcattaag acacactcat tgttcttatt tactagcaaa
     3181 aggtgtatct atacattaca tttctaaaag attaggtcat aaaaatatag caataactac
     3241 atccgtgtat tctcatttgt tagaagaaaa atttaatgaa gaggacaaaa aaacaactaa
     3301 aattttagaa agtatgtaat ttagggaccc attagggact ccaaacccaa taaatactgt
     3361 tgttacaagg tttctatgta tccaaactgg gggcaatata aacgcgctga tttaatcgga
     3421 caatcttctt atattaaaaa taatgatgtc gtaatattca atgaagcatt tgataatggt
     3481 gcatcagaca aattattaag taatgtgaaa aaagaatatc cttatcaaac acctgtactc
     3541 ggccgttctc aatcagggtg ggacaaaact gaaggtagct actcatcaac tgttgcagaa
     3601 gatggtggcg tagcgattgt aagtaaatat cctattaaag aaaaaatcca gcatgttttc
     3661 aaaagcggtt gtggattcga taatgatagc aacaaaggct ttgtttatac aaaaatagag
     3721 aaaaatggta agaacgttca cgttatcggt acacatacac aatctgaaga ttcacgttgt
     3781 ggtgctggac atgatcgaaa aattagagct gaacaaatga aagaaatcag tgactttgtt
     3841 aaaaagaaaa atatccctaa agatgaaacg gtatatatag gtggcgacct taatgtcaat
     3901 aaaggcactc cagagttcaa agatatgctt aaaaacttga atgtaaatga tgttctatat
     3961 gcaggtcata atagcacatg ggaccctcaa tcaaattcaa ttgcgaaata taattaccct
     4021 aatggtaaac cagaacattt agactatata tttacagata aagatcataa acaaccaaaa
     4081 caattagtca atgaagttgt gactgaaaaa cctaagccat gggatgtata tgcgttccca
     4141 tattactacg tttacaatga tttttcagat cattacccaa tcaaagccta tagtaaatag
     4201 tgctcaacta actaataact tgcttcgttc taaaaggacg aagcgagtta tattgttaaa
     4261 atttgaattg acttacattt taataaaatc atcttaacaa ctttaatttt tcattaatac
     4321 aagtctttac tctacactca aacaagattc atacactgca cgtcataata aatctatcta
     4381 ttcaaatata aataaaagtt acctactaca ttctatgtag caggcaactt ttattactta
     4441 tttcttttca ttatcattaa gtacttttac aaacttcaca ttatgtgtct tccaatcaac
     4501 ttcatataat gctgataatt tttcttcttt tttatctaca tggttttcac cagaccaata
     4561 gccccagaaa ccatggcgat tccaatctat tttaaactca tccattgatc ttttataatg
     4621 aacaacaaat tgtgatttac ctttgtcttt tttatcatgt gacataacag ctaaaaattc
     4681 tggattaaac ccttcagaca cagttacagg cattttgtct ttaggtgtga aattatcttt
     4741 cgcccataaa tttccatttc gtgttaaaga aaaaatttca cttttagttc tattatcact
     4801 atcattagtt aattgtctcg tatggtcatg tcccatatta tttatcaaat gtgcttctac
     4861 tttccaacct acacctttat gtgacgtaga ttgatcaagt aatgtacgat atgatggttg
     4921 ttgataacta atcgtctctg aataattact ctcttttgta atatttccag ttaagcctcc
     4981 acgattaatc gaaaaatctc cacctgtttt ataaccatac gtatatttaa cttctcttga
     5041 ttcatcctga ttttttggtg caaagtcagt cacatttgta ttgttgttgt catcaacatt
     5101 ttgaattgaa actgaataag atccaggcca tcttaatgta ctattccaat aaccatttgg
     5161 gtctaaaatt ctcaaaccac taccaattgt accttttgct ttaataaata ctgtttcttt
     5221 atcataatta ggttcagtta agaaattaaa ttgtaagctt tgagtaatat ttttttgact
     5281 atcacttgtt gtagctgtac gtgtatacat tttagtatca ccatcaagat tcttctcaga
     5341 aacttgtttg atttcagaat taatctttgc aaatgaagta gctggtaata cagtgaaagt
     5401 agtcgatagt gctaacgtac aaattgtgat atttttacat agttgtttaa tcattagtaa
     5461 tccgcccttt caatattatc cttctttata aggtttattg tcatcagaat atttatcaac
     5521 gactttaact gttttatttt tccaatcaac ttcataagtg acaattaatc tttgaccatc
     5581 tttatttttt tctaaaattg gaggtgcata atgtattcca ggtctgtttt tcaaaatatc
     5641 ttgatttcgt gtgtatgtta cttcaaattg cgttttctca tttgactttt cattagataa
     5701 ataagttaaa aattctggat taaagccact tcttactaat gctgggtatc tatattttga
     5761 agcaaagctt agttcagggt tttctacagt agcaattctc gtatttctat agaataataa
     5821 ttcatcattt ctatttttca cttctccacc atacttcaag tcattcgcaa taactgacca
     5881 gtgtacatgc cagttattat ttttaccgct ggcaattgtg tcataatttt gctgattata
     5941 actaatcgtt ttggagtagc tatttgatga agttcgtcca ataccttttg ttgaatcgaa
     6001 tttaccacct gagctatatg aaaatgtact gtctactttt gcagttgaaa ttttattttt
     6061 cggcaattgg tctaatattt cagttttacg atttcttttt acttgaaaat ctacatggta
     6121 ctcacttgga tactttaacc aatttgaatt tttttcttct ttatgagatt caaactttaa
     6181 atttgaatga attgagcctt gttttttaac aagtaataca ttcttgtcat aagttggatc
     6241 atcgataaag tcgaattgta aattttggag tatatttgtt ttctcatcat atactgtttc
     6301 agttcgtttt gtgattttac cgtttttccc aatatcatcc ggtgctgttg aatttttatc
     6361 tttattagta acattacgtt tgtctttttg ttgagactta tcaacatgtt ctttcttatt
     6421 ttggtcttga gagtctttat gagctgaatt tgcttgagtc gttgctgctg ataacaataa
     6481 aattgcacat gataatgatg acgctattaa aacacgtttt ttatttttca ttgttataac
     6541 cttctttcgt atgattgata tttgttgata tgtatcgaca tgtgaataat atcacaaaaa
     6601 cagagaatat atatttaact atttattaaa tgattttgtt aatattatta aatactttat
     6661 cctctttaaa aataatgtgt gtacaaagtc attaatttag caaatatttt tatttagtag
     6721 ttaataacca tcgatttgaa a
//
LOCUS       DAKTIP010000140         6679 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAKTIP010000140 DAKTIP010000000
VERSION     DAKTIP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.141"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(40..1098)
                     /locus_tag="O0305_002049"
     CDS             complement(40..1098)
                     /locus_tag="O0305_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCY0275178.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(1085..1291)
                     /locus_tag="O0305_002050"
     CDS             complement(1085..1291)
                     /locus_tag="O0305_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275179.1"
                     /translation="MLDHTNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(1516..1860)
                     /locus_tag="O0305_002051"
     CDS             complement(1516..1860)
                     /locus_tag="O0305_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0275180.1"
                     /translation="MNRNRMKQIILEYIKNNDSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWMGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLRLPIVKNKNIKI
                     DHWLPVTFSLVN"
     gene            complement(2531..4903)
                     /locus_tag="O0305_002052"
     CDS             complement(2531..4903)
                     /locus_tag="O0305_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCY0275181.1"
                     /translation="MAIKKKDRIIGVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQSDICDNQEVLNTLVDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKIKDLLQGTYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KAIFFVGDGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(4995..5243)
                     /locus_tag="O0305_002053"
     CDS             complement(4995..5243)
                     /locus_tag="O0305_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCY0275182.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDAIKEGFSYHERRIHNDQMFDALEKASSFADQSQDNA"
     gene            complement(5244..5627)
                     /locus_tag="O0305_002054"
     CDS             complement(5244..5627)
                     /locus_tag="O0305_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0275183.1"
                     /translation="MEIKQKYQLSKVVKILEVVLYEEDKFQSDKDYHYQDKALYEYAL
                     KLVHNGLFNILAELDFEDEAFLILDEVTMTLSDVMKETQHVYRYSVIDEKGEHKHTTD
                     RKGHVIGMLEWALDYIVGNIEVEEL"
     gene            complement(5629..5832)
                     /locus_tag="O0305_002055"
     CDS             complement(5629..5832)
                     /locus_tag="O0305_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0275184.1"
                     /translation="MRKQVIITKTVVGWYNIKDTQHNLMLNIPPKVFEQYFPDVSKDV
                     QVACLEMDLSKITEIKNKKKVGS"
     gene            complement(5799..5972)
                     /locus_tag="O0305_002056"
     CDS             complement(5799..5972)
                     /locus_tag="O0305_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0275185.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMVTYGFNLTGE
                     ITTCENKLLLQKQ"
     gene            complement(5984..6256)
                     /locus_tag="O0305_002057"
     CDS             complement(5984..6256)
                     /locus_tag="O0305_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCY0275186.1"
                     /translation="MSRTKLHDVPAKENTITEPKQVVVNPLFAKPNALASILGISYSS
                     VNRILKEWEKDSKGVDDLYYSLSSTMIVISIPRFEEYMKARHKKWM"
     gene            complement(6249..6512)
                     /locus_tag="O0305_002058"
     CDS             complement(6249..6512)
                     /locus_tag="O0305_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275187.1"
                     /translation="MVLNLKRLRAERIACGITQDEMAHKMGWKTRTPYAKRENGIVDI
                     GANEFIKMAKILGYETNNLDIFFTNNVPGKERKNILKGGELNV"
BASE COUNT         1977 a         1246 c          798 g         2658 t
ORIGIN      
        1 atgggcaggg ggctataaaa ttttatttaa ataatttttt tatttaatat taactatgcg
       61 caataactct ggaattattt ttatatcaaa cacatagttc aatgcaaatt gataaatacc
      121 ttttaatgct tgtttctttt tgttatacat aataggttcg tatttagcat atatggcatg
      181 tccaaagtct ccaacaaaga catcatgtgc atcatcagta accactacat tagtcacatt
      241 aaataggtta cttaaatcac ctgcaagtcc acctaatccc atctgtgcca actctttgat
      301 gagcttatca tgatgttctg tgttcatcac aatagataca ttacgcctaa acgtttgtgg
      361 tatatctttc atcgcttgct tgataccatc gtatattgat gtgttactta cttctttgat
      421 accttgatta taaagtgata agtcttggtt acctttatca attaaaccac ttgaattatt
      481 cttatctaat aacaatgttg tttcatatgc gacacactca ttaataaata tctctttaag
      541 tagatcatct aatgttctat cagagccatg aactacactg tctgatactt ctgtgtagat
      601 atgtccctca cgtctatcaa agtatttact actgccatta agtgatagct caataccgtt
      661 agtagctaac ttatctttaa tgtatgttag tgatggtata gattcaacat gttcatcatc
      721 agtaatggta atcacctcac gtagtgattg aggtagctca caattcacca ataactcttt
      781 aaatacttct ttacgtgcag ttggtaactc ttgaatatta aagttattct cactactaac
      841 taattgtttt gttaaggtac ctacccatgt attacttgat tgatttactt cattcatatt
      901 taacactcct aataattttt tgttaatttg ctctggtaat aaaacttctg tttcagtaat
      961 acttaattgc tttaacccct gttcaatcat acttacatct ttacctatat ccttttgata
     1021 tagattggca tatcctttaa tatccatatc atcaaagtta gatagttgat acccaaattg
     1081 ttctttaaac tctttcataa tcttcatctc ctaatttgtt tttagtatga acaactaaac
     1141 catatatacg ttcgttgttt ttatcttctt catagaacga aatgttatga agaaagtatt
     1201 tgtctaataa ttcgtgatac ttatttaata gttcttgctt atctctatac ttaaacacac
     1261 gttttacaga ttcatttgta tgatctagca cgtcttgcct ctctcctttt aatctgatag
     1321 atatacattt taataagttc aaaatcagca cgcttttttg tatagcgcct tacaacgcga
     1381 tttatatatt tcgtattcat taattgaacg ttcacaggtg tatgtttgat tatttgatat
     1441 gcgattcttt tatgattaat aaatatacac actcccatat ctatacttga tattttaaat
     1501 gcttatctgc atgttttaat ttactaaact aaatgttact ggtagccaat gatctatttt
     1561 tatattttta ttctttacaa ttggtagcct tagaccttta ccatcaataa gatagtacat
     1621 tggcggacaa atttgcattt caattagccc gtcgcgtttt aaatctgcca caatgttaaa
     1681 cgcttcttca ttccaaccca tccaaaatat aatattctta tggttgccac tggtatatgc
     1741 gccgttacct ttgtatttaa aaccttgttc ttcaaataca ttctcaatct ctacaaatga
     1801 agtgctgtca ttgttcttta tatattctaa gatgatttgt ttcattctat ttctattcat
     1861 gttactctag tcctccaatc tatccagtta ctaaagcaac cacgttagat actaaaaacc
     1921 actgaattta tgtttttcaa taaccactgt aaacacagtg atgtcaatac atacaggctg
     1981 ttggttactg agttactgaa aattcacggt attttatata atcttttatt accttaattc
     2041 tctattatac atttctacta aaaagaaata atttatagta actcagtaac tttttgtcaa
     2101 aaagactaat atgaaagtaa aaactgtggt tactgaattc tagaatttta gtatctttta
     2161 gtgacttaaa cgttttttag taactttagt atcttaggca attttgtata actgcaagtc
     2221 tcttccgatt atgtgcaact gaaatttaca gctatgcaac tcgttttaaa ttttccagtt
     2281 gcaatttttt tcttactgtc acaaggggtt aatccacttt tgcaactatg caaccgaaat
     2341 cacacaaatt agtttatata atttttgata ctatttctta tttatatttt ggttatttac
     2401 ttttctacta ataagttata gattttgagt tgctcagttg cataagtgcc ctaaaccctt
     2461 gatataactg tgattatagg tgcaacccgt tttaaaattt tcggttgcat tcggttgcat
     2521 tcggttgcaa ctatattgct tttagcttca ccttttcata cgatttatag ttttttccac
     2581 tttcaatagg ttcaatgtaa tttctattgt cattaaagtt gtatatcctc ttagcaagtt
     2641 cttcattgtc atatcttcgc tgtgcatcag ttttccaata attctctaaa taatgttcaa
     2701 attgtttata aaacttgttt gaactcaatg cattatagcc attttcatca caatattctt
     2761 tataaaatgc gtatacaata tatttcggca cttttctaat agtccattga tcaaacatat
     2821 ttactttaaa cccataaact ggatcgttat cttctttaaa tacttcaagc atttttttag
     2881 atgcatcagg aatgtcaaag gtttcaaaat ctaaattaat tgctttataa agcacatact
     2941 ctagcacttg ctgatttttt atatagtctt ctttgatttt aaaattctct ttaatgccat
     3001 taaaattggc attaaacggt actatcaata accttcttaa agtcccgcct gttttgtctt
     3061 taaatttagg cattccattc gttgattgaa taactgtaca cttaaaagtc gctctatata
     3121 agggtttatt tttaaactca accaacactg gatcgccagt aactacactt ttaaagtttg
     3181 aagaatcatc gacatacaca ccaactggta catcgtctcc aattactgct gtcttgccct
     3241 ctaacacact caatttaaaa cgttcatcaa actcattcac ttttaagcta gcaatattgc
     3301 tataacctat tacattagac aataattctt gaaacgtccc tttaccatta tttccatcac
     3361 caacaaagaa aatagccttt ttacgagtgt agttaccatt cattgagtca ttaattactt
     3421 gccacaataa tttaacaacc tgattatcat tacaagcaat ctcttctatc caccgatcta
     3481 tattccagcc atttatttca ggtactatat cttgccttac atacgatgta tctattttac
     3541 tggtaaatat ataatcaggt gtaaatgatt ctagttgctt cgttttacgg ttaaatacac
     3601 catttttaac tggtattaag tatggtgagt tagttttctc ttttatatct accatattgg
     3661 ttaaatgata aataacttca tcagctttgt tgctattatg tttgggctct aaataggaaa
     3721 tcactcgttt tataattgta gtgttctgtg tatatatccc ttcatcaaat tgatacatag
     3781 ctaacttagt attttcttca tcatcaaaaa gtataaatgt taaatgctca ttcaaaatat
     3841 atgcacattt tatagggcta attgttgtag gctttcgccc tcgttttcct tcttcaatcc
     3901 aattttcttc catttttgtt ctttcttcat gccccaattg attcaacaac tgtttaacat
     3961 ctttactccc ctgtggtata acattaaatt catctatcga tttagtataa attgtctttt
     4021 gattctttat agctttatct aactcaagct gtccccaagt cgtattacct cgtttacttt
     4081 cccatttatc cgtaagatta ttgtagttta aaaatatacg ctccatttgc tgtttgtttt
     4141 tacctgtgta aaacgctaag tagtgtaata agctttgtac tgcttcgctt gaactctcaa
     4201 aatatgattc atatgtgcct tgtaatagat ctttaatttt atctttttgt ttagatttca
     4261 tcataatgtt tataatatct tcatcagata attgtatatt ggtattagat tcctctctca
     4321 caacctcatt tgctggcaaa ttctctttaa aatattcatc aactagagta tttaatactt
     4381 cttgattatc acaaatatca ctttgaccaa ttgtgcatcc tgtaacagtc ataaaccttg
     4441 ctgaatcata taactctatg tctaaatctg ttcttttctt tttacggtta tctggtagtt
     4501 tacctttaaa gaaacaatgt aatcctgtac cacttggaga cttttcacaa tatgtgagtt
     4561 gcatcatttt tagtgctaat tcagaattga tttgtccttt tttatcaatt gcattatcaa
     4621 tatctaggca aacaaaatta ttaccatcac ttaatacaaa acctataccg ctatattgct
     4681 catcaacttc atattcaata cttacacttt caaagtcaca ccatgttttt ttattggttg
     4741 tactagctct gtaaccatta atactatatg gtacttttcc aaaattttgt tgcttctcgt
     4801 tccattcagc tcgccataat acccaattgg gcactaattt taattcttgc ggtatttcta
     4861 attctttaac tccaataatt ctatcttttt tcttaattgc cacagaatgc ctcctttcta
     4921 atctgatttc tctgtatttt caatgcaact aatgttaaaa tattaatggt atgattttat
     4981 aaatttttat ttttttatgc gttatcttgg ctttggtcgg cgaaagatga cgctttttct
     5041 aatgcatcaa acatttgatc attgtgaata cgtctctcat gatatgaaaa tccttcttta
     5101 atagcatcgt ctttatttaa ggtatgattt ggctcatgta gatataaatc ttcaaaatgc
     5161 gagccatgat tatcctttaa atcctcaaac ttttctttca atatctttaa atcacacatt
     5221 aaatttttaa tttcccaatt catttataat tcctccactt caatatttcc cacaatgtaa
     5281 tctaatgccc actctaacat tccaatcacg tgtcctttgc gatctgttgt atgtttatgt
     5341 tcaccttttt cgtctatgac actataacgg taaacgtgtt gtgtttcttt catgacatca
     5401 cttagcgtca ttgttacttc atcaagaatt aaaaatgctt catcttcaaa atctaattca
     5461 gcaagaatat tgaacaatcc attatgaact aactttaaag catattcata taatgcttta
     5521 tcctgataat gatagtcctt atcggattga aacttatctt cctcgtataa tactacttct
     5581 aatattttaa ccacttttga taattgatat ttttgtttga tttccatctt aactacctac
     5641 ttttttctta tttttaattt ctgtaatttt tgataaatcc atttctaaac acgcaacttg
     5701 aacatcttta ctaacatcag gaaagtactg ttcaaatact tttggcggta tatttaacat
     5761 taaattatgt tgagtatctt taatgttgta ccagccaact actgtttttg taataataac
     5821 ttgttttcgc acgttgtaat ctctcctgtt aaattaaatc cataagttac catcatgccg
     5881 tacacactaa aagcgacata catgttagat attgctagta ataatattgt taacaatgaa
     5941 actaagcaga tataagttaa gtacattttc attgccttgc ctcctacatc cattttttat
     6001 gacgcgcctt catgtactcc tcgaatcgcg gaatactgat aacaatcatt gttgatgata
     6061 acgagtaata taaatcatca acacctttag aatctttttc ccactctttt aaaatgcgat
     6121 tcaccgaact atatgaaatt cccaaaatac tagctagtgc attaggtttc gcaaacaaag
     6181 gattcactac aacttgcttt ggttctgtaa ttgtattttc tttagctggt acatcatgca
     6241 attttgttct agacatttaa ttcacctcct tttaagatgt tttttcgttc ttttccggga
     6301 acgttattgg taaaaaaaat gtctaggtta tttgtttcat aacctaatat ttttgccatt
     6361 ttaataaatt cattcgctcc aatatctact attccatttt ctctctttgc ataaggcgtt
     6421 cttgttttcc accccatttt gtgagccatt tcatcttgcg taataccaca agctattctt
     6481 tccgctctca atctttttaa atttagtacc acgtttacac ctcctatcgt tctcgttcga
     6541 gaactaatta caatttacca tttacgttct cgttcgtcaa caatttttac ttaaaaaaat
     6601 tcaaaaagtt ttttcttcct tatatattgt attcttttgg gaacggtgtt ataatcaaat
     6661 cgttcacaaa taagaacaa
//
LOCUS       DAKTIP010000141         6652 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAKTIP010000141 DAKTIP010000000
VERSION     DAKTIP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.142"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(1..498)
                     /locus_tag="O0305_002059"
     CDS             complement(1..498)
                     /locus_tag="O0305_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCY0275188.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(508..768)
                     /locus_tag="O0305_002060"
     CDS             complement(508..768)
                     /locus_tag="O0305_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275189.1"
                     /translation="MIIMRGYNGRYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(1150..1263)
                     /locus_tag="O0305_002061"
     CDS             complement(1150..1263)
                     /locus_tag="O0305_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275190.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKIISRNSI"
     gene            complement(1922..3922)
                     /locus_tag="O0305_002062"
     CDS             complement(1922..3922)
                     /locus_tag="O0305_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275191.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
     gene            complement(3919..4674)
                     /locus_tag="O0305_002063"
     CDS             complement(3919..4674)
                     /locus_tag="O0305_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275192.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(4782..5669)
                     /gene="nsaS"
                     /locus_tag="O0305_002064"
     CDS             complement(4782..5669)
                     /gene="nsaS"
                     /locus_tag="O0305_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCY0275193.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(5680..6345)
                     /gene="nsaR"
                     /locus_tag="O0305_002065"
     CDS             complement(5680..6345)
                     /gene="nsaR"
                     /locus_tag="O0305_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCY0275194.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQNQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(6371..6571)
                     /locus_tag="O0305_002066"
     CDS             complement(6371..6571)
                     /locus_tag="O0305_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275195.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQDKEQKDNKG"
BASE COUNT         2176 a         1135 c          872 g         2469 t
ORIGIN      
        1 ttaagatgaa gattcttgca ataatatttc tatatttgtc gatatatcca atggatcttc
       61 cattggcaag aatcgattta caacatttcc ttgtcgatcg attataaatt ttgtgaaatt
      121 ccattttatt ggggacccaa agattcctgg ttgttcattc tttaaatgcg taaataacgg
      181 atgttcatcg ttcccgttca cagatatttt agctaacact ggaaatgtta cgccaaattt
      241 ctcacgacta attttcaaga tttcttcatt agaacctggt tgtcgattgt caaaattatt
      301 attcggaaaa ctcaacacta caaacccacg atccttatat ttttgaaata aagtctctag
      361 ttttttcaat tgttcgctat atatacattc tgttgcagta ttaacaacta aaatcacttt
      421 acctctaaat gcttctaatt tataagttaa gcctttataa tcacttactt cgatatcata
      481 cacatttcta ttattcataa gacaccccta cacagccttt tttatattga atatgttctt
      541 tttagaatgt tctgataaaa taagtgcgcg tttacaccgt gaacacacat tatatagcgt
      601 gatacatttt tcgagcacac gataaataat gttcgagttt atgttgttgc tcaacctatc
      661 cgatttaccg tcttttttca cctgtcttac tttattaaaa taatcacatt gtatagctgt
      721 attagtccag ctacaatatc ttccgttata acccctcatt ataatcatcc ttattttcta
      781 tttttaaaaa gacaattaga ccgctcttta aactatagat taatacttaa gttaaactca
      841 tacatactga taccatacgt tagatctaac aatttaaaat tcgttataac tatggattaa
      901 agagctgccc aactcatata atccttaaaa acttcacatg tgattgttta ttaagccctc
      961 ctttatctta ttaaatatcc ttataaccct tttaaaatta aactgacaca ctcatacatt
     1021 gttacacgcg acgataaacg tcatattatt gcatatcatt aaaacaagtc actttgtatc
     1081 gtgtgcgacc gaatacatat taaatcatca ataatgatta ttcaattatt aaaaataaaa
     1141 acgaacattc tatatagagt ttctacttat tattttttca gcattgtcta cattacttaa
     1201 cattcgtgtt tgtaagtaat attgaccgcc aatatttaga cactttataa gtatgccatt
     1261 catcattttt aatcatatta tttttcttat tgatatttct attcatatat ttctgattgt
     1321 atatttagat ataataattt gttattggaa aatatatgaa acaaatttga ctcatctttt
     1381 tcaatatcta aatgacacga tgcatgattt tttaattttg taattaactt ccttttaaat
     1441 tagactgaat tgtgacaatt ttcaaacatt ttaatgtgta aatcattggc aaaatatacg
     1501 aattgcattt aaatcgtcaa tttacatata tttttgcgta gtaatcaatc gttatcattg
     1561 ttatttatcg ttacattatt tcgagtatca gtatgtattt cgggcttcgt ttgataacga
     1621 catttctttg tgacatcgct tcatcagtgt aacaacaaat acaatgattt cgtgatgtta
     1681 gttacccatt ttatgtgttg cataaaatat gttgttataa aacattttaa atcattttat
     1741 ataaacaatc tatatatttt tggcatttcc aaaatatcac ttgttatatt aaaaaccgac
     1801 aagacatttt atcttatcgg ttgaaatttg ttattgttat ttgtaatgtt tttaggtttc
     1861 tttttaatat aatatatttc agtgaaaata catgattgat tgtgatttca ctgaaacatg
     1921 gttaattgcg ttgttgatga ataactttag cataaatata ggaagttatt ttgtacatcg
     1981 ccatatatag aaacgaaatt ataatgacag ctagtacgta acttgttaaa aatatatgat
     2041 ggttattaat acctatcata tttagtaacg tatatacaat gttactagaa attaatgtgt
     2101 gaatcagtgc tactgttatt ggtattgcga acaagaaagt catttgattt cgtgttatct
     2161 ttgctattct tccattatct aaaccaagtt ttttcataat gtcataattg tgctgatttg
     2221 cataggcttc tgagacttgt ttataataca tcattagaaa tattccaatg acaagtacaa
     2281 ttgatactac gccacctaca aatattagtc cacctgtcaa acgatcccaa accattagca
     2341 tttctgattt agaatttatt gacgcatcgt attttttcga tagcttttgt atttgtgatg
     2401 ttgttaattt cgtctttcca aatacattaa agtttatcga tattgtcgaa ttcttttgtt
     2461 catgtgcata aaatcctttt actttttgta attgattgtt atctttaaca attaaaacca
     2521 tactatcttg aatcatcatc aaatttaacg catgtgcgtc aagttgtttc actttaaaga
     2581 cttcattatt aagaccgacc ttagactgct ttttaaaaat aggtacattc gtaatcatac
     2641 ctatactttg attgtctaag tgtaatttgt tttggtgata tcgattataa tcttgtgcag
     2701 taaaagctgt aatcattact gattgtcttg aaaatatcgc gccttcatta ccaatgaagt
     2761 ctattggaat atcatcacta gcctgtttta acttgtaata agcacgattc ttttctaaag
     2821 aagctctaaa taatgtcgtt tcatatactt tgaagtcttt tgcattaacc gaaccctgta
     2881 tatcacgttt aatattctca attgtttgtt gacgttctat ttgtgactta gaattgtcgc
     2941 taaatgacaa atcataatca ttcgtaatca atttcgtaat ggtatgattc atatcacgat
     3001 aggttgttaa tgtcattgtg agcgttacaa tcaaaaatgt acacagcaaa gtgattgttg
     3061 caagactgac tgcattcatt ttaagacgta cacgcatccc aactaccaca aaaaaatacc
     3121 gtggatgata gtaaactttt gatacctgct gcaatatact aataattatt tcacttatac
     3181 ctacaaaaaa tgcataagta ccgataataa ctaatcctat gacaatccat attttaaaaa
     3241 aggcaccaaa cgtcgtatct tgttgtaatg caataaagta acctaaatat agtgctgcgc
     3301 ttcctataac aattaaaacg taacgcaacc atcgtgatat gacactagaa tcggaacgat
     3361 gttgataagt tatcggccgt tgaaaattaa tactaaacaa gttgaatata agtagcatgc
     3421 ccattaatac agcaatgata atcaaagtaa taaacatcgc tacagagtca aatggataat
     3481 accttaacgt cgcaacttca ctgcccatta ttttctgtat aaacaagaaa aatatcgcac
     3541 caagtaagta tccgccggca atactaacga ctgaaattat cacaaattgt aagatagttt
     3601 ccatcactac aattaaacgc atacttttct tggtcatgcc caatgtcata aaaatgctaa
     3661 actcttttcg tctttgtgac atcataaagt gatttgcata gaaaataaaa ataaaagtta
     3721 aaagcgccat aaaaaaatta gctatgataa taaatggtac taggaagtca ttcttctgtt
     3781 ttatgtagct atttaaccca attgacacaa gcgtatattc aatcatgaat agtacactta
     3841 ccgctaaaat gaatggaatg attacaagtc gctgcgtaat aaattgacgt cgcactatgt
     3901 gccataacaa tcttaacttc atatattgac acttcctcca ttcacaagtg ctaagctatc
     3961 tgttattcgt tgttgaaaag ctaattgact ttcttcacca cgatatattt catgatatag
     4021 acgcccatct ttaataaaaa tgactcgctc tgcataagac gcatcgatat ttgaatgagt
     4081 taccattaaa attgtctgtt ccaattgatg aatctcttga aataacatca tcaatgcctt
     4141 tgatgttttt gaatcaagtg cacctgtagg ttcatcggct agtaaaatcg tcggcttagt
     4201 aactaatgca cgtgcaatgg caatgcgttg cttctgaccc ccagagattt cagaaggata
     4261 cttgtttaag aatccctcta aatgtaattg tactgccaac tgatgtaccc tttgttctat
     4321 atcttttcgt ttagcaccag ctaaaattaa tggcatcatt atattttctt tattcgtcat
     4381 tgttggtaaa agattaaaat cttgaaaaac aaaacctacc atttgttgac gatacaatgc
     4441 tttactttta tttttcatat tatttaaatg tgcgccatcc acaataatgt caccttcagt
     4501 tagtccatca aaagaagcaa ttaaatttag tagtgtagac ttccctgacc cagactcacc
     4561 cataattgca acaaattcac cagctccaac tgataaattc atttgattaa gtgctgtcgt
     4621 agcattcaaa cctttaccat aaaccttttt tacatgtttc acttctagca acatggcatg
     4681 cacctcattt ctttgttagt tctatattaa aacaaaaaaa tactaataac atttaaatta
     4741 gtaaaaaaat gttactagta tacttacaat attgaaagtt tttattcatc tggaaattga
     4801 atcgtaaatg ttgtaccctc attttgttta gatacgactg aaacaggatg gtttgtgtgt
     4861 gttgaaattt gttttacgat aaataaacca atcccacttg agttactttg gcgctggcca
     4921 ttataacctg aatagccctt atcaaatatt ttaggcaagt ccgcttcact aataccgata
     4981 ccattatctt ttacgtgtaa ttgattggat tgctcatcaa attcaatcca tatatcttta
     5041 cctctcgcat acttaagtgc attatttatt aattgttcta tcattaaaga ggtccatcta
     5101 acgtcagtta atacttcgtg atgacaaggt tcataatgga tttttgtttt ttgatcaata
     5161 aactgtattg aatatttcat aataattggg cgaatgatat tattaatcga aattttagtg
     5221 acagaaatat cagaagtttc atttaataac tttaaataac taagtgctaa acttgtatag
     5281 ttatcaattt gaataacctc ttgacgaaca cgattaacaa cattaggctc atctctttca
     5341 agtaacagtt gtgctgcagt aatgggtgtt ttcatttgat gtacccatgt taaaaagtag
     5401 ctctctacat catttttata ttcaatttgt tcatttttaa gctgatacaa cgcattttct
     5461 aagttttcaa tttgttgttg ttggcttata gtttttacaa aacttaaata tttaatacct
     5521 atgaatatga gtaataataa taaaataacg ccaagtgcta gtaaatatgc ttctaatggc
     5581 aaatgataca ggtaaaacat taagccaaac aaagcaaaaa taactatgac tattgctatt
     5641 tcctgagtaa tacttttaag aaaggtcatt gcattcaccc tatactttat atccgacatt
     5701 tttctttgta atgataaaat cattaacgcc aatagtattt aattttttac gcaggcgcgt
     5761 catgttaaca gctaatgtgt tatcatctat gaagttttct gattcccaac atttttcaat
     5821 taaagcagtt ctacttacat atttatcttc attttgaaat aataacttta atatttgtaa
     5881 ttcagtcaaa gatagctgta tgttttgttc ttgatacacg acttttgctt catctaatat
     5941 taatgtacaa cctttaaccg tcaatgaatc attagctact gacaagtcat aagttcgtct
     6001 caataatgct tgaattttgg caatcgttaa tgacaagtta aatggctttt cgataaaatc
     6061 atctcccccc atttgtattg ccataatttg gtccatatta tcaatacggg aactaataaa
     6121 tataattggc acattagatg tttttcggat ttcttgacac caatgaaaac catttaacgt
     6181 tggcaaatta atatctagca atacaagctg aggttgattt tggttaaaga tagacagtat
     6241 atcatcaaat tgttcaacga caataacacc gtaattccat tttttaagtt cagatgctaa
     6301 actctctgct ataacaaaat catcttcaac aattaatatt ttcatctata atcttcttcc
     6361 ttcaattgaa ttatcctttg ttatcttttt gttctttatc ctgctgcaat gacggctctt
     6421 gatttgaact ttccgtgtca tttgtatttt gatttagtgt atttgctttt tctttctcga
     6481 gttgcttttt cttcgtacgt ttacgctctt ctaaaataga caacactata aagaatatga
     6541 ttaataatgg agataacatt actagtatca tttttccacc tcaaattata tttaattcta
     6601 tattttcaca cgcttctatt ctaacgcttt ctataacaaa ttgtcatata ta
//
LOCUS       DAKTIP010000142         6536 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAKTIP010000142 DAKTIP010000000
VERSION     DAKTIP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.144"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            63..2681
                     /gene="mutS"
                     /locus_tag="O0305_002067"
     CDS             63..2681
                     /gene="mutS"
                     /locus_tag="O0305_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCY0275196.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            2694..4703
                     /gene="mutL"
                     /locus_tag="O0305_002068"
     CDS             2694..4703
                     /gene="mutL"
                     /locus_tag="O0305_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCY0275197.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            4709..5251
                     /locus_tag="O0305_002069"
     CDS             4709..5251
                     /locus_tag="O0305_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCY0275198.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            5716..6534
                     /locus_tag="O0305_002070"
     CDS             5716..6534
                     /locus_tag="O0305_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCY0275199.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
BASE COUNT         2502 a          865 c         1219 g         1950 t
ORIGIN      
        1 ttacaaacga atatattgta aagacaaatt ttaaaaagag acaagggttg tgaagaaata
       61 atatgtctaa tgttacacca atgatgcagc aatatttaaa aataaaatca gaataccaag
      121 attgcttatt attttttaga ctaggtgatt tctatgaaat gttttatgaa gatgccaagg
      181 aggcatcacg tgtacttgaa attactttaa ctaaaagaga tgctaaaaaa gaaaatccaa
      241 ttccgatgtg tggtgttccg tatcattctg cagatagtta tatagataca cttgttaata
      301 atggatataa agtagctatt tgtgaacaga tggaagatcc gaaacaaacg aaaggtatgg
      361 ttagacgtga ggtagtaaga attgtgactc caggaactgt gatggagcaa ggtggtgtag
      421 atgataaaca aaataactat attttaagtt ttgttatgaa tcaacctgaa attgcgctta
      481 gttactgtga tgtttctact ggcgaattaa aggttacaca ttttaatgat gaagcgactt
      541 tattaaatga aattacgacg ataaacccta acgaagttgt tatcaatgac aatatttccg
      601 ataatttaaa aagacaaatt aatatggtga cagaaacaat aacagtcagg gaaacgttat
      661 catcagaaat ctatagtgtg aatcaaactg aacataaatt aatgtatcaa gcgacacaat
      721 tattgctaga ttatattcat catacacaaa aacgtgattt atcgcatatc gaggatgttg
      781 ttcaatatgc agctatagat tatatgaaaa tggattttta tgctaagaga aaccttgagt
      841 taacggaaag cattcgatta aaatcaaaaa aaggaacgct actttggcta atggacgaaa
      901 cgaaaacacc aatgggagca cgccgcttaa aacaatggat agatagacca ctaataagta
      961 aagaacaaat tgaagcacga ttagatatcg ttgatgaatt tagtgctcat ttcatagaaa
     1021 gagacacctt aagaacatat cttaatcaag tgtatgatat tgaacgtctt gttgggcgtg
     1081 ttagttacgg aaatgttaat gcgagagatt taattcaact taaacattcc atttctgaaa
     1141 taccgaatat taaagcatta ctaaattcta tgaatcagaa tactcttgta caagttaatc
     1201 aactagaacc ccttgatgat ttacttgata tattagaaca gagtttagta gaagaaccac
     1261 caatttcagt taaagatggc ggactattca aagttggttt taatacgcaa ttagatgaat
     1321 atcttgaagc ttcaaaaaac ggaaaaacat ggttagcaga attacaagcc aaagaaagac
     1381 aacgtacagg aataaaatca ttgaaaataa gctttaataa agtgtttggt tattttatag
     1441 aaataacacg tgccaacttg caaaattttg aaccaagtga atttggttat atgaggaagc
     1501 aaacgttatc gaatgctgaa cgttttataa ctgatgaact taaagaaaaa gaagatatca
     1561 ttttaggtgc ggaagacaaa gccatcgaat tagaatatca attatttgtt cagctacgtg
     1621 aagaagttaa aaaatatact gaacgtttac aacaacaagc taaaattatt tcagagctag
     1681 attgtttaca gagctttgca gaaattgctc aaaaatataa ttacactagg ccttcattta
     1741 gtgaaaataa aacattagaa ttagtggaat ctaggcaccc agtagtggaa agagtaatgg
     1801 attataatga ctatgtgcct aataattgtc gattagataa tgaaacattt atatatttaa
     1861 ttacaggtcc gaatatgtct ggtaaatcga catatatgag acaagttgcc ataattagta
     1921 taatggccca aatgggagct tatgtccctt gtaaagaggc agtgttacct atatttgatc
     1981 aaatattcac tagaataggt gcggcagatg atttggtttc aggtaagagt acgtttatgg
     2041 tagaaatgct agaagcacaa aaggcattaa cttatgcaac agaggatagt ttgattattt
     2101 tcgatgaaat tggacgtggt acttcaacgt atgacggttt agctttagcg caggcaatga
     2161 tagagtatgt agctgaaaca tcacatgcta aaacgttatt ttcaacacat tatcatgaat
     2221 tgacaacatt agatcaagca ttaccaagtc taaaaaatgt tcacgtcgct gctaatgaat
     2281 ataaaggtga acttatattc ttgcataaag tcaaagatgg tgcagttgac gatagttatg
     2341 gtattcaagt tgcgaaatta gctgatttac ctgaaaaagt tattagcaga gcacaagtga
     2401 ttctaagcga gtttgaagcg tctgctggta aaaaatcatc gatatcaaat ttaaaaatgg
     2461 tcgaaaatga acctgaaatt aatcaagaaa atttaaactt aagtgttgaa gaaacaactg
     2521 atactttatc tcaaaaagac tttgaacaag catcatttga tttgtttgaa aatgatcaag
     2581 aaagcgagat tgaactacaa attaaaaatt tgaatttatc taatatgaca ccaattgagg
     2641 cattggtgaa gttaagtgaa ttacaaaatc aattaaaata gaggtgttgc aaaatgggga
     2701 aaattaaaga actccaaacc tcattagcaa ataaaatcgc agcaggtgaa gtagttgaaa
     2761 gaccgagttc tgttgtgaaa gaactgttgg aaaatgctat agatgcaggc gctacagaaa
     2821 taagcattga agtagaggaa tctggcgtcc aatctattcg cgtagtcgat aatggaagcg
     2881 gaattgaagc ggaagactta ggattagtat ttcatagaca tgcgactagt aaattagatc
     2941 aagatgaaga tttatttcat attaggacat taggattccg tggtgaagca ctagccagta
     3001 tttcatcagt tgctaaagta acattgaaga cttgcacgga taatgctaat ggaaatgaaa
     3061 tatatgtaga aaatggtgaa atattaaatc ataagcctgc aaaagcgaaa aaaggaacag
     3121 atatacttgt agaatcatta ttttataata caccagcacg tttaaaatat attaaaagtt
     3181 tatacactga actaggtaaa ataacagata ttgtcaacag aatggcaatg agccatccgg
     3241 acattcgaat agcactcatt tcagatggca aaacaatgtt aagtacaaat ggttcaggac
     3301 gaactaatga agtgatggca gagatttatg ggatgaaagt tgcacgagat ttagtacata
     3361 tatctggaga tacaagtgat tatcacattg aaggttttgt tgcaaagcct gaacattcta
     3421 gaagtaataa gcactatatt tctattttta ttaatggacg atacattaaa aactttatgc
     3481 taaataaagc gattttagaa ggctatcata cactcttaac aataggtagg ttcccgattt
     3541 gttatattaa tattgaaatg gatccaatct tagtagacgt aaatgttcat ccaacaaaac
     3601 tagaagtgcg tttatcaaaa gaagagcaac tataccaatt gattgtgagc aaaatacaag
     3661 aagcatttaa agaccgtata ttaattccta aaaataactt ggattatgtg ccaaaaaaaa
     3721 ataaagtatt acattcattc gaacaacaaa aaatcgaatt tgaacaaaga caaaacacag
     3781 agaataacca agagaagacg ttttcatctg aagaaagtaa cagtaagcca tttatggaag
     3841 aaaatcaaaa cgatgagata gttataaaag aagattcata taatccattc gtaacgaaaa
     3901 catctgaaag tttaatagct gatgatgaat cttctggtta taataataca cgtgaaaaag
     3961 atgaagacta cttcaaaaaa caacaagaaa ttttacaaga aatggatcaa acatttgatt
     4021 caaatgatgg tacaactgtg caaaattatg agaataaagc gtctgatgat tattatgatg
     4081 taaacgatat taaaggaaca aaaagtaaag accctaaacg aagaattcca tatatggaaa
     4141 ttgttggaca agtacatgga acgtatatta ttgctcaaaa tgaatttggc atgtacatga
     4201 ttgaccagca tgcagctcaa gaaagaataa aatatgaata ttttcgagat aaaataggtg
     4261 aggttaccaa tgaagtacaa gatttattaa tcccgttaac atttcatttt tcaaaagatg
     4321 aacaattagt cattgatcaa tataaaaatg agcttcaaca agtaggtatc atgttagaac
     4381 attttggtgg tcatgattat attgtaagta gctatccagt ttggttccct aaagatgaag
     4441 tagaagaaat tattaaagat atgattgagc taattttgga agagaaaaaa gtagatatca
     4501 aaaaattacg tgaagatgta gcaatcatga tgtcatgtaa aaaatccatt aaagcgaatc
     4561 attatttaca aaaacatgaa atgtctgatt taattgatca attaagagaa gcggaagatc
     4621 catttacatg tccacatggt cgtccaataa tcattaattt ttcaaaatac gaattagaaa
     4681 aattatttaa gcgtgtgatg tagagaggat gaatcaagtg aataacaaca tattgcctgc
     4741 tataagaaac attaaagatt tagagaaact gattaaaaca gactataaaa tgtgtgtgct
     4801 tctagatatg catataggac atataaaaag tattatggaa ttgctgaagc aaaatcatat
     4861 agagtgtttt attcatatag atttgataaa aggtttaagc cacgatgaat ttgcaagtga
     4921 atttattatt cagcaataca agccaaaagg tatcgtatcg actaaatcta aagtaataaa
     4981 aaaagctaaa tcattaaata ctttaacgat ttttagagta tttattattg atagtcaagc
     5041 attgaaacgc agtatagatt tgataaaaaa agttgaacct gattttgttg aagtacttcc
     5101 aggtgttgcg agtaaagcga ttcatcatat tcagaaagaa acaaacacac aagtcattgc
     5161 aggtggccta attaatacaa tagatgaagt caatgaagct gttaaaaatg gagcgaaata
     5221 tgtaactact agttatgata aactttggta aatcataagt ctgcaaaaat ttcatttttc
     5281 acgagattta gtcatataac ataataaaaa agccgttttc atatgggata tgcagtaggt
     5341 atcaaacaaa aaataatcaa ataaaataat tagcacctct aaaacgatta aaatcattaa
     5401 agttttagag gtgtttatta gtaaaaaatg caactagtca taaaaaatat ggctgaagaa
     5461 tgtcaattaa acaaaagagc gtcataaata aattagttag gattggtatt tctagttttc
     5521 aaattaaact acttttataa agacttgaaa aattaaattt gacaaaattt ttacaaaaac
     5581 cattgacaac gctttcatat cgatagtatc ataacaaata taataaaagt taatacatag
     5641 aatagagacg ggagatttct acgagccaaa ctgctagtgt aggaatctct ttgtcttttt
     5701 gggaggacat ttaatatgaa tgtatattta gcagaattcc taggaactgc aatcttaatc
     5761 ctttttggtg gtggcgtttg tgccaatgtc aatttaaaga gaagtgctgc gaatggtgct
     5821 gattggattg tcatcacagc tggatgggga ttagcggtta caatgggtgt gtttgctgtc
     5881 ggtcaattct caggtgcaca tttaaaccca gcggtgtctt tagctcttgc attagacgga
     5941 agttttgatt ggtcattagt tcctggttat attgttgctc aaatgttagg tgcaattgtc
     6001 ggagcaacaa ttgtatggtt aatgtacttg ccacattgga aagcgacaga agaagctggc
     6061 gcgaaattag gtgttttctc tacagcaccg gctattaaga attactttgc caacttttta
     6121 agtgagatta tcggaacaat ggcattaact ttaggtattt tatttatcgg tgtaaacaaa
     6181 attgccgatg gtttaaatcc tttaattgtc ggagcattaa ttgttgcaat cggattaagt
     6241 ttaggcggtg ctactggtta tgcaatcaac ccagcacgtg atttaggtcc gagaattgca
     6301 catgcgattt taccaatagc tggtaaaggt ggttcaaatt ggtcatatgc aatcgttcct
     6361 atcttaggac caattgccgg tggtttatta ggtgcagtgg tatacgctgt attttataaa
     6421 catacattta atattggttg tgcaattgca attgttgtag ttattattac tttgatttta
     6481 ggttacattt taaataaatc atcaaaaaaa ggtgatatcg aatcaattta ctaaaa
//
LOCUS       DAKTIP010000143         6487 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAKTIP010000143 DAKTIP010000000
VERSION     DAKTIP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.145"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1170
                     /gene="ftsW"
                     /locus_tag="O0305_002071"
     CDS             <1..1170
                     /gene="ftsW"
                     /locus_tag="O0305_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCY0275200.1"
                     /translation="YPLLVTYIVLSLIGLVMVYSASMVPATKGTLTGGIDVPGTYFYN
                     RQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIVSLLLLTLVIGKDINGSKSW
                     INLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKLILSPIVLALGCTFLVFLQK
                     DVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLVVFVIALMAGWLPSYLTARF
                     STLTDPFQFESGTGYHISNSLLAIGTGGVFGKGLGNSAMKLGYLPEPHTDFIFAIICE
                     ELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVGIATYFGSQTFVNIGGISAT
                     IPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQQRKKQQQKVDIRRQFN"
     gene            1724..5176
                     /locus_tag="O0305_002072"
     CDS             1724..5176
                     /locus_tag="O0305_002072"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCY0275201.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFNIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(5316..6227)
                     /locus_tag="O0305_002073"
     CDS             complement(5316..6227)
                     /locus_tag="O0305_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCY0275202.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
BASE COUNT         2294 a          959 c         1205 g         2029 t
ORIGIN      
        1 tatccgttat tagttacata tattgtattg agtttaattg gattagtgat ggtatatagt
       61 gcaagtatgg ttccagcaac taaaggcaca ttgactggtg gtatcgatgt tccaggaacg
      121 tatttttaca accgacaatt agcatatgtc ataatgagtt ttataattgt attttttatt
      181 gcatttttaa tgaatgttaa attactgagt aatattaaag tgcaaaaagg tatgattata
      241 actatcgtct cactattatt actgacgtta gtaataggta aagatattaa tggttctaaa
      301 agttggataa acttaggatt tatgaactta caggcatctg agttattaaa aattgcaatc
      361 atattatata tcccatttat gatcagtaaa aaaatgccta gagtattaag taaaccaaaa
      421 ttaattttaa gtcctattgt attagcatta ggttgtacgt ttttagtttt cctacaaaaa
      481 gacgtagggc aaacattact aatattaatt attttagttg cgatcatttt ttattcagga
      541 attggggtaa acaaagtcct aagatttggt ataccagcag tgctaggatt cttagtagta
      601 tttgtcattg cattaatggc tggttggtta ccaagttatt taactgccag atttagtacg
      661 ctaacagatc cattccaatt cgaatcagga actggatacc atatttccaa ttcattgctt
      721 gcgataggta ccggtggcgt atttggaaaa ggattaggaa atagtgcaat gaaattgggc
      781 tatttaccag aaccacatac agattttatt tttgcaatta tttgcgaaga attaggttta
      841 atcggaggat tgctagttat tactttagag ttctttattg tatatcgtgc cttccagttt
      901 gcaaataaaa catcatcata tttttataaa cttgtgtgtg ttgggattgc cacatacttt
      961 ggaagtcaaa cgtttgtaaa cattggcggt atttcggcaa caattccatt aactggtgtg
     1021 ccattgccat ttatcagctt tggtggatca tcaatgatta gtttaagtat tgctatgggt
     1081 ttacttctga ttgtaggtaa acaaatcaaa gtagaccagc aacgaaagaa acaacaacaa
     1141 aaagttgata taagaagaca atttaattaa aaaaatacta gccaatattt agtacgagat
     1201 gacttctgtc attaagcaat gctaaacatt ggctatttat atactctttg tcaaattgga
     1261 ctgatgagtt caataacgga agttaggcaa ccaaacaacg cttaacttcc tttttgtttt
     1321 gatattacga gattactcat gattatcaaa ttttataaac aaaacaacgt ataattataa
     1381 ctaaatttta tgactaaaaa ttactaaatg tggtggcgaa attagaatca tgatgataag
     1441 ataaagttat cgctattgat aataaatgtt gatgttttgc aattttattt ttaatttttt
     1501 agagttgtag aaatcgaaaa tgtttcacaa caaaaacgtg aactacctgt tgttgcatag
     1561 tcatttttga aattattatt tctgaagcgg aggttttaat tgtctcaaga atgttaaatt
     1621 ttgaaaattt gaaacataac tatagcaaaa cagagcgctt taagataaaa ttttattatc
     1681 taattagtta gaaaattcta atagtttgag gagtgtatcg ctattgaaac aaataaaaaa
     1741 gttacttgtt gctaaccgtg gagaaattgc aattcgtata ttcagagcgg cggcagaatt
     1801 agacatcagc acagttgcaa tttattcgaa tgaagacaaa agttcattac atagatataa
     1861 agcagatgaa tcctatttag ttggaagtga tttaggtcct gctgaaagtt atttaaatat
     1921 tgagcgtatc attgatgtag caaaacaagc gaatgtggat gcgattcatc ctggctatgg
     1981 atttttaagt gaaaatgaac aatttgcgcg tcgttgtgca gaagaaggaa ttaaatttat
     2041 tggtcctcat ttagaacatt tagatatgtt tggagataaa gttaaagctc gtacaacggc
     2101 tatcaaggca gatttaccag ttattcctgg tacagacggt ccaattaaat catatgaatt
     2161 agcaaaagaa tttgcagaag aagctggttt cccgctaatg attaaagcca caagtggtgg
     2221 cggcggtaaa ggtatgagaa tcgttcgtga agaaagtgaa ttagaagatg ctttccatag
     2281 agcaaaatca gaagctgaaa aatcatttgg taatagtgaa gtttacatag aaagatacat
     2341 tgataatcca aagcatattg aagtacaagt cataggtgac gaacatggaa atatcgtaca
     2401 cttatttgaa cgtgattgtt cagtacaacg tcgtcatcaa aaagttgtag aagttgcacc
     2461 atcagttgga ttatcatcaa cattacgtca acgtatttgt gatgctgcaa ttcaattgat
     2521 ggaaaatatt aaatatgtca atgcaggtac tgttgaattt ctagtatctg gtgacgaatt
     2581 cttctttata gaagttaacc ctcgtgtaca agtagagcat acaattacag agatggtaac
     2641 aggaattgat attgttaaga cacaaatttt agttgcagca ggtgccgatt tatttggtga
     2701 agagattaat atgccgcaac aaaaagatat tacaacatta ggctatgcca tccaatgtcg
     2761 tattacaaca gaagatccgt taaatgattt catgccggat actggaacaa tcattgctta
     2821 tcgttcaagc gggggctttg gtgtacgtct tgatgctgga gatggtttcc aaggtgctga
     2881 gatatcacct tattatgatt cattactcgt aaaattatct acacacgcga tatcatttaa
     2941 acaagcagaa gaaaaaatgg tacgctcatt acgagaaatg cgtattcgtg gtgttaaaac
     3001 taatattcca ttcttaatta atgtaatgaa gaataaaaag ttcacaagtg gtgattacac
     3061 aactaaattt attgaagaaa caccagaact tttcaacatt cagccgtctc tagatagagg
     3121 tactaaaaca ttagaatata taggtaatgt aacaattaat ggtttcccaa atgttgagaa
     3181 acgtccgaaa ccagactatg aattagcatc aattccaact gtatcttcaa gtaaaatcgc
     3241 ttcatttagt ggtacgaaac aattgcttga tgaagtaggt ccaaaaggtg tagctgaatg
     3301 ggttaaaaag caggatgatg tcttactaac agatacaacc tttagagatg cacaccaatc
     3361 attattagct acacgagtta gaactaagga tatgattaat atcgcatcca aaacagcgga
     3421 cgtatttaaa gatggtttct cactagaaat gtggggcggt gctacatttg atgtggcata
     3481 taatttcttg aaggaaaacc catgggaacg acttgaacgt ctacgtaaag ctattccaaa
     3541 tgtattattc caaatgttgt tacgtgcttc aaacgcagtt ggttataaaa actatcctga
     3601 taatgttatt cataaattcg tacaagaaag tgctaaagca ggcatagatg tctttagaat
     3661 tttcgattca ttaaactggg tagatcaaat gaaagttgcc aatgaagcag tacaagaagc
     3721 gggcaaaatc tcagaaggta ctatttgtta tacaggtgac attttaaatc ctgagcgatc
     3781 aaacatttat actttagagt attatgtcaa actagctaaa gagttagaac gtgaaggttt
     3841 ccatatttta gcgattaaag atatggcagg cttattaaaa cctaaagccg cttacgaatt
     3901 gattggtgag ttaaaatcag ctgtagattt accaattcat cttcacactc atgatacaag
     3961 tggtaatggt ttattaacat acaaacaagc aatagatgct ggtgtcgata tcattgatac
     4021 tgctgttgct tcaatgagtg gtttaacaag tcagccaagc gccaattcgt tatattatgc
     4081 attaaatggc ttcccacgcc accttagaac tgatattgaa ggtatggagt cacttagtca
     4141 ttattggtca actgtacgta cttattattc agactttgaa agtgatatca aatcaccgaa
     4201 tactgaaatt tatcaacatg aaatgcctgg tggacagtat tcgaatttaa gtcaacaagc
     4261 taaaagttta ggtttaggcg aaagatttga tgaagtcaaa gatatgtatc gcagagtgaa
     4321 tttcttattt ggtgatatcg taaaagtaac accatcgtct aaagtagttg gtgatatggc
     4381 actttatatg gtacaaaatg atcttgatga acaatccgtg attacagatg gctataaatt
     4441 agatttccca gaatcagtag tgtcgttctt caaaggtgaa ataggacaac ctgtaaatgg
     4501 ttttaataaa gatttacaag cggttatttt aaaaggccaa gaagcactaa cagctcgtcc
     4561 aggtgaatat ctagagccag ttgattttga aaaagtccgt gagttgcttg aagaagagca
     4621 acaaggtcct gttacggagc aagatattat tagttatgta ctatatccaa aagtatatga
     4681 acaatatatt caaactagaa atcaatacgg aaacttatcg ttacttgata cgcctacatt
     4741 cttctttgga atgcgtaatg gtgaaacagt agaaatcgaa atcgataaag gtaaacgatt
     4801 aattattaaa ctagaaacga ttagtgaacc agatgaaaat ggtaatagaa cgatttacta
     4861 tgcgatgaat ggtcaagcga gacgtattta cattaaagat gaaaatgtgc atacaaatgc
     4921 gaacgttaag ccaaaagcag ataagagtaa tccaagtcat atcggtgctc aaatgccagg
     4981 ttcagtaact gaagtcaagg ttagtgtagg tgaaactgtg aaagctaatc agccgttgct
     5041 aattactgaa gctatgaaaa tggaaacaac aattcaagca ccatttgacg gtgtgattaa
     5101 acaagtaact gtaaataatg gtgacacaat agcgacaggc gatttattaa tcgaaattga
     5161 aaaagcaact gactaaaatg attaaaataa aacgagatta cacaacaatt gacgtgtaat
     5221 ctcgttttta catggcttag gtttaatttt aaatattgat ataaaaaacc gtaattttaa
     5281 tgggcgtgcc attaaaatta cggtttgctt ttttgttatt gcttgtcact tcttactgat
     5341 cgtaacatta gcatgataaa gtatgttgtc ataccaaata aataagtgat aaataatgca
     5401 tggaaaagtg cgattatcag gttaacattt gtcataatag ataatgcacc tgtgataact
     5461 tgtaatataa caagtataaa tgcagctgta taaccataat gaacagtacg gttatttgga
     5521 taatttttaa cagcgtgaat ataagtaatc ataataatcg taaacactat aaaagccatg
     5581 atacgatgcg tgagttgaac ccaatcttgt tctgaatgtg gtacaagatc gtggaatggc
     5641 aatggccaac caccatatgc taaacttgca tccgcatgtc tcactagtgc accagtataa
     5701 acaccacaat aaatgatgat tgccattaac catgttaaac gtcttaatgg ctttttgata
     5761 tataattcgt cagcttcata tttttgatct atagagaaaa taatcaatgt tattaaaaat
     5821 acagatgaga aactgattaa tgatatacca aagtgcaatg ctaaaacgta atcgttttgt
     5881 tgccaaataa cagcagcagc tccgattaat gcttgcaata ataagaatcc aacactaatg
     5941 attgataaag gtttaatttc tttaatatag cctatatgtt tccatgcagt gataactaac
     6001 cataagacca ttaataaaga caaagctgaa acggctctat gacttaactc aataatcgta
     6061 tcaataggaa agaattctgg aatcaacgca ccatgacata gtggccaaga agaaccacaa
     6121 ccatcagctg atccggtttt ggtaactaag gctccaccaa gttgtacaaa tgtcatcatt
     6181 aacgttgcta cgacacctaa ccattttaaa ttctttttgc caaacaattt atacacccca
     6241 tcataaaaaa acgcatcact gagaattaga attaatagcc ggatttgaga tgatactata
     6301 aatagacctg attgtaggat atgaaaagat gggatccaac gcaggaaata tgatttaatt
     6361 ttacaagtat tgtatgtgga cgacataaaa agaacttaat cgtaatgatt tttttattgg
     6421 gataattatg taatccacgt ttactcaaat ttgaagctta aatattaatc tcttttagat
     6481 gcatgat
//
LOCUS       DAKTIP010000144         6475 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAKTIP010000144 DAKTIP010000000
VERSION     DAKTIP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.146"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            140..1234
                     /locus_tag="O0305_002074"
     CDS             140..1234
                     /locus_tag="O0305_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCY0275203.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(1324..1416)
                     /locus_tag="O0305_002075"
     CDS             complement(1324..1416)
                     /locus_tag="O0305_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275204.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(1425..3161)
                     /locus_tag="O0305_002076"
     CDS             complement(1425..3161)
                     /locus_tag="O0305_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCY0275205.1"
                     /translation="MIKRYLQFVKPYKYRIFATIIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(3452..3994)
                     /locus_tag="O0305_002077"
     CDS             complement(3452..3994)
                     /locus_tag="O0305_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCY0275206.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(4296..5333)
                     /gene="mutY"
                     /locus_tag="O0305_002078"
     CDS             complement(4296..5333)
                     /gene="mutY"
                     /locus_tag="O0305_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCY0275207.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            5485..6462
                     /locus_tag="O0305_002079"
     CDS             5485..6462
                     /locus_tag="O0305_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0275208.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
BASE COUNT         2120 a         1139 c          921 g         2295 t
ORIGIN      
        1 gttttacatc gctatcagta ttatgtatga tttattttaa caactataaa taagcttgaa
       61 ttttgtaact agtaagtgta taacagtaat gaatacgaat attagatatt caataaactt
      121 aaaaggggcg tcattatttt tgagactagg agctcggatt tttaaaactg gtatagccat
      181 tattttagct atgtctatcg cttctttact accggatgat gttggtctga aagccttagc
      241 tggtgtcagt gctgttgttg caatgcaacc tagtatttat cgttcattta aaacagtttc
      301 tgatcaagca ttgggtaaca ttataggtgc catactatct gttacaatgg taacaatttt
      361 tagtgataat tttatcatta tgggtgttac cgtcatagta cttattgcta ttttatttaa
      421 atttaattta gcacatgttg ctactttggc gagtgtaaca gcattaatca ttatgggaca
      481 acataccggt tctttctata ttaccgcctt ttacaggttt gtacttgtaa tgattggtgt
      541 cataagttca tctctagtta actttgtgtt tctaccacct aaatttgaaa caaaaattta
      601 ctataattca ttaaatatat cctcagatat ttttatgtgg tttaaattag tattaaatga
      661 tacgacggag tttaataata ttaaacaaga tagtcataat ttaaaacaac gtgtcgaaaa
      721 gttagagaaa atttatgatt actatagcga agaacgtcct attacaaaaa aacatattca
      781 ccaacagaat agaaagaaaa tacttttcag agaagtagtt cagacgacta gacaagctta
      841 tgaagtatta aacaaattgt cgcgttatca aaacgactta tatttactta ataacaattt
      901 cctattacaa atcaaattag atttagattc attaactgct tttcacgagc aaattttagc
      961 tagcctttct aaaaaagcac gttataatgt gacacatgtt gattatgagt tagataatcc
     1021 gcagaaaaaa gatttattgt ccacatttca gcatgagtta ataaatcatc cttatcaaac
     1081 ggaatattcg tttgccaatg ttatgcaaat tgttgcagcc atcgaagagt atcgacacca
     1141 tttagagcat ttagaccgca taagaattag ctttttcaca tatcatagat ctgatgcaga
     1201 cattgaaatt gttgaagaag actttgattt ataacattaa aacaatttta tcaattaatg
     1261 ccacaatgag ataaactaat attgaatctc gaaattaaat ttaaaaaagc agtaagatta
     1321 ttttcaatta agaaaatctc ttactgcttt ttctatgtta tgaccccacc atcctgttac
     1381 aaattttctc ttcaagattg tactgaagtc aaacaactaa tactttataa gttttgaatg
     1441 ctatataaat gctcgtaagc accttgtttt gcaatcaatt cacgatgcgt acctgtttca
     1501 acaatatgtc cattttcaat tacgacaatt ttgtcagcat gtgtaatagt ggacaagcga
     1561 tgcgctacga taagtgtcgt tcgatcttta ctcaacacat ctaatgcttc ttgaataatg
     1621 gattcacttt ctaaatcaag tgcacttgtt gcttcatcca agataagaat tggcggatta
     1681 tttaaaaata ttctagcaat cgataatctt tgtttttgac cacctgataa tttaacacct
     1741 cgttcaccta cttcagtgtc atatccctgt ggcaagttca taataaagtc atgtgcatta
     1801 gccattttcg ccgcttcaac tacttcttca tctgttgctg ttggacgacc aagtaaaata
     1861 ttttccttaa ctgtatcaga gaataaaata ttatcctgtt gcaccaatcc tatttgattt
     1921 cttaaacttc ccgttaaaaa atctttaatg ttgtgaccat ctattaaaat ttgcccagaa
     1981 gttacatcgt aaaatctcgg tattaagtta attaatgttg atttaccacc accactcata
     2041 cctacgaaag caactgtttc tcctttttca atactcaaat taatatcttt taaaattgga
     2101 gcttcgttat cgttatattg aaaactaaca tgatcaatat caatacgacc ttgtttaatt
     2161 tcaataggtt gagcaccaac accatttttg atgtcataat cttcatcaat taattggaat
     2221 acacggtcca ttgaagcaaa actttgcgtt aaagttgtaa atgatgcgac taaacgacgt
     2281 aaaggcccga acaataactc taagtatcca acaaatgctg caagtgtacc tactgtgatt
     2341 gatccagaaa tagcaagata tgcaccaaca ccgatgacaa taattggtcc aatatctgta
     2401 actgtattaa ttgcggcaaa ggaataggca ttccatcttg tatgtttcaa cgcacgtgtt
     2461 aggaaattag tattcttttt atcaaagttt ttcgcttcat tgtcttcaat cgcaaaactt
     2521 ttaacgactg aaataccttg aacacgttca tgcaagaatc cttgaacctc agctaatgct
     2581 tgagatcttt cacgtgtcaa ttttcttaat cttccaaaga aaacgtacac cgttaaaatg
     2641 taaaatggga agataaacag tgctgctaaa gtcaatttca catctaaaaa gaacattatg
     2701 gatagtgcaa taataattgt tatacaatct aaccaaatat tcattaaccc ggttaaaatg
     2761 aaatcttttg tttgttcaac atcattaatc actctagata ttacttgacc tacttgatta
     2821 ttagcataaa atctcgcact taaagcttgt aaatggttgt ataacttttt acgtatatca
     2881 tacaatattt tattacttgt ccattgcgcc aaatattgac gtataaattc aattggtggt
     2941 ctcactatta caaaaataaa taatgcgata ccaatggcaa tagttaaatg atgaactttt
     3001 tcatcagtcg ttagtgcgtg gttattaatc acgccatcta ttgcatattt aattaatagt
     3061 ggtataagca ttggtatacc aaacttaatt atcccaacaa taatcgttgc aaaaatacga
     3121 tatttgtatg gcttaacaaa ttgcaaatat cgtttaatca tacaatcccc cctaatctat
     3181 tgccctatcc tattcataag cataaaaatg aatagaggtt ggatacataa tttgtagatg
     3241 taaattcttc ttacaattta catttttaaa attaagatat ctcaacctct gtcatattca
     3301 tgtgattaaa tacgttgtct tattcgtatg ttatattcta ttcaatttaa tctatggata
     3361 ctgtgtcccc acacgacagc aaaagttatc atacttcttt acatcactaa gtcaatataa
     3421 atgatttaat cagtatttac actttatttg cttaatactg tctaattttt ttgtaacgtt
     3481 ctttccaaac tttgataaaa tctggcgcga atgggccctt cttctgttct atccattgtt
     3541 gaagaatgtc cacgttgcgt cttaaaataa tatcaatatc atgcggataa ttcatttgat
     3601 tcatatgttg ctcatattca tcttcatcta ataaatgata ctttccgttt ggatatactt
     3661 taatatctaa atcatagtct atatatttta atgcctcttc atcacaaaca aatggtgatg
     3721 acaaattgca atagtaataa attccatctt ctctaaacat gcagataaca ttaaaccaat
     3781 attctgagtg aaagtaaaca attgccggtt cacgtgttat ccaagttctt ccgtcacttt
     3841 cagtcactaa cgtatgatca tttccaccaa tgacaacatg atcagtaccc tttaatattg
     3901 ttgtttcaga ccaaacgcga tgaatcttac catcatgttt ataactctga attttaatgt
     3961 tttccccttc tttaggtatg gattctctga ccatactcca caccaccttc tgttaattta
     4021 accattataa attatagcat atttcagaaa tagtattata taaatacata tttttacgaa
     4081 ataagatttt actacttaat aattaaactc ggtaatattg ctaagtacta caacagagat
     4141 ttacatgtcc catttaaagt atataaaatc atcactttta tatatcaaca ctttaacttt
     4201 ttgacattgt tattctatga gatttaaaga tatcatttat actttttaaa attaatgtca
     4261 ctatgttttc cgataatatt accaatcatc gaatgttacc catttataaa ttgataaatc
     4321 tttgacatag gtacagggaa tgtatattga tctcgatcac ttaaatcaaa ccaaatcatg
     4381 tcatctggta atgtttcaat gttaattgct cctgaaacgg cgtatacttt aatcttccat
     4441 gttaaatgag taaattgatg cttcaactca aaaataggtg tttctactgg ttgaatgtca
     4501 tgaccgattt tttcagtcat tttacgtcta gcatgctcac tttcaaacat aggaaattgc
     4561 cacataccat gcaataattt ttcgctacgc ttttgcaaca gatattgacc ttgattattt
     4621 ctaattaaaa agacggattg ctcaattact tttttactta catttttaga tttaacaggt
     4681 aacttttcaa atgtaccttt atcaaatgcc tcacagtttt cttgaactgg acaaaataag
     4741 cataatggat tttttggtgt acaaattaac gcccctaatt ccatcatagc ttgattaaac
     4801 gttccagctt ctgtagtaac atacggtaat aattcttgtt cgtacgattt cctcgtcgat
     4861 tgtaatttaa tatctcgata gtcatcattc aatctagacc atactcgaaa aacatttccg
     4921 tctacagttg ctagtggtac attatatgca atgctcatta ctgcagcttg tgtgtatggg
     4981 ccaacacctt ttaacgcttt aaattgatca ggatctttgg gaactaagcc ttcatattta
     5041 tcatgaactt ctttaatcgc cgtatgaaaa tttcgagctc tactataata tcctaagcct
     5101 tcccaatact ttaacacttc atcttccgaa gcttgactca aaacttccac agttggaaat
     5161 cgttcaacaa aacgatgata atagtcaata actgttttaa cttgtgtctg ttgtaacatg
     5221 acctcactta accaaatata gtacggattg gtcgtttgtc gccatggcat ttctctttga
     5281 ttttcatcaa accagtgtat caaattttct ttaaaactag actgctgata catttataaa
     5341 accctttcct caccaaaatt aattgtcttt actcataatg tttttattgt acattaaaat
     5401 catggttagt atgtaagtta atttagttat ttgcgaaatt ggattataat agtatatata
     5461 atattatgaa atgagtgaac tgatatggac actgcaacac atatcgcaat tggggtgggc
     5521 cttacagcac ttgcaactca agatccagca atggcttcta cgtttggtgc aacagctaca
     5581 acccttatcg ttggttcatt aattcctgat ggggatactg ttcttaaatt aaaggacaat
     5641 gcaacatata tttcgcatca tagaggtatc acgcattcca tccctttcac aatactatgg
     5701 ccaattttaa ttacattttt aatattcacg ttctttagtg gaaccaaccc atttcatgta
     5761 tggatgtggg ctcagctcgc agtattttta catgtctttg tagatatatt caattcttat
     5821 ggtacacaag cgcttagacc tatcacaaac aaatggattc aattaagtgt gattaacaca
     5881 tttgacccta ttattttcac agttctttgt attggtattg tattatgggt tataggcttg
     5941 catccatttg cagtcttctt tcctataatc gctttactaa tcatttatta catgattcgt
     6001 tttaaaatga gagccgtaat taagcaacaa gctttaaaag caattcaaca agagcatcac
     6061 cctgttaaag tatttgttgc gccaacaata aaatttatgg aatggcgtgt cgcgatacaa
     6121 actgatgcac atgactatgt tggaaaagca tatggtagga atgtggtgtt tagtgataaa
     6181 gtggaacgtc aaacattatc aacagactcc attttatgga aagtcaaagg taataaagat
     6241 atacgtactt ttttaaactt ttcatcaatc tatcgttggc aaacaacaac gttagcagat
     6301 ggttctactg aaattcgttt gattgatttg cgttatttaa aaaatgatca ttattcattt
     6361 gtggcaattg cacatgtaac aaacgataat gtcatagacc actcttatat tggctgggta
     6421 tttacagaag ataagttaca acgtaaactg tatgctaaat aatttcaagt tatta
//
LOCUS       DAKTIP010000145         6471 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAKTIP010000145 DAKTIP010000000
VERSION     DAKTIP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.147"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            51..1682
                     /locus_tag="O0305_002080"
     CDS             51..1682
                     /locus_tag="O0305_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCY0275209.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGIIVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNYNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1679..3352
                     /locus_tag="O0305_002081"
     CDS             1679..3352
                     /locus_tag="O0305_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCY0275210.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLIVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKKRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSVRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAITRMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3479..3922)
                     /gene="mgrA"
                     /locus_tag="O0305_002082"
     CDS             complement(3479..3922)
                     /gene="mgrA"
                     /locus_tag="O0305_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCY0275211.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            4149..5075
                     /locus_tag="O0305_002083"
     CDS             4149..5075
                     /locus_tag="O0305_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCY0275212.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            5178..6086
                     /locus_tag="O0305_002084"
     CDS             5178..6086
                     /locus_tag="O0305_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCY0275213.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIVQDIP"
     gene            6121..6408
                     /locus_tag="O0305_002085"
     CDS             6121..6408
                     /locus_tag="O0305_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275214.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFVKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
BASE COUNT         2195 a          896 c         1141 g         2239 t
ORIGIN      
        1 taatttgatt ttaaggaaaa ccttttataa taggtttagg tgatataatt gtgaaaaaat
       61 taacaacaat actgtttcaa tataaaattt ttccggtact catgttcttg gtcagtactg
      121 gtctcggcat aatcgttata acgcaaaata ttttaatagc agatttttta gctaaaatta
      181 taagacatca atttcaaggt ttatggattg tattatttat tttattaggt gttttacttt
      241 taagagcaac tgtgcaattt ctaaatcaat ggttaggtga tacattagca tttaaagtta
      301 agcatatgct tagacagcgg gttatttata aaaataatgg tcatccaatc ggtgaacaaa
      361 tgactatact cacagaaaac attgatggtc tagcaccttt ttataagagt tatttgcctc
      421 aagtgttcaa atcaatgatg gttccgctca tcataatcat tgcaatgttt ttcatccatt
      481 tcaataccgc attaattatg ttaataactg caccatttat tcctttgttt tatattattt
      541 tcggtttgaa aacgcgagat gagtcaaaag atcaaatgac ttatttgaat caatttagtc
      601 aacggttttt aaatattgct aaaggtttag tgacgttaaa gctatttaat cgtacagagc
      661 aaacagagaa gcatatttac gacgatagta ctcagtttag aactttaaca atgcgcattt
      721 tacgcagtgc ttttttatcg ggattaatgc tcgaatttat aagtatgtta ggtattggat
      781 tggttgcatt ggaagcaacg ctaagcttag tagtatttca taatattgat tttaaaactg
      841 cggcaattgc gattatttta gcgcctgaat tttataatgc aattaaggac ttagggcaag
      901 cgttccatac tggaaaacaa agtgaaggtg ccagtgacgt tgtgtttgag tttttagaac
      961 aaccgaacta taataatgaa tttctattaa agtatgagga aaaccaaaag ccatttattc
     1021 agttaacaga catatcattt cgatatgatg attctgatag attggtatta aatgatttaa
     1081 atttggaaat atttaaaggt gatcaaattg cacttgtagg tccaagcggg gcaggtaaat
     1141 ccactttgac acatcttatt gcaggtgttt atcagccaac aataggtact ataagtacaa
     1201 accagcgtga tttaaatata ggaatactta gtcaacagcc atatattttc agtgcttcta
     1261 taaaagagaa tattacgatg tttaaagata tagaaaataa tactattgaa gaagtgctag
     1321 acgaagtagg tttattagac aaagtgcaat ctttcacaaa aggcattaac acaataatag
     1381 gtgaaggagg cgaaatgtta tctggtggac agatgagacg catagaactt tgccgtcttt
     1441 tagttatgaa gccagatctc gttatatttg atgagcctgc aactggttta gatattcaaa
     1501 cagaacacat gattcagaac gttctgtttc aacattttaa agatacaacg atgattgtca
     1561 ttgcacatag agataataca attcgccatt tacaacgacg cttgtatata gaaaatggaa
     1621 gactgattgc tgatgatcgc aatatttcag taaatataac agaaaatggt gatgacttat
     1681 gaaaacacga ctaaaatttc aagtagataa ggatttattg ttagctatag ttgttggtgt
     1741 ttgtggaagt ttagttgcgc tcgccatgtt tttcttaagt ggttatatgg tgacacaaag
     1801 tgcacttggt gcgccactat acgctctgat gattttagtc gttacagtaa aattgtttgg
     1861 gtttttaaga gctattactc gatacgtaga gcgccttatt tctcataaag ctacatttac
     1921 aatgctacgt gatattcggg tacagttttt cggtaaatta gtaaatgtca ttcctaatgt
     1981 ttaccgtaaa ctgagttcta gtgatttaat ttcacgtatg attagtcgtg ttgaggcatt
     2041 acaaaatata tatttacgtg tttattatcc accagtcgtc atcggtttga cagcgctagt
     2101 tacagtcata gttttggcgt tcatttcaat cggccatgcg ctattgatta tggttagcat
     2161 gttgttcact ttactcattg ttccttggtt aagctcaaaa aaagcacgta ctttaaagaa
     2221 acatgcagct aatgaacagg cccgattttt aaatcatttt tatgattata aagctggtat
     2281 ggatgaacta cgtcgattta atcaaattaa tcattatcga gataatttga tggctaaatt
     2341 aaatcatttt gataaattac aacttaaaga gcaacgcttt ttaacgattt atgattttat
     2401 attaaatatt attgctatgc tttcgatttt tggtagttta gttctaggat taattcaaat
     2461 taatgcaggc caactaaata ttatttatat gacgagtata gttttaatgg tcttaacttt
     2521 atttgaacaa gctgtaccaa tgacaaatgt cgcgtattat aaagcggata ctgaccaagc
     2581 attgcacgat attaatgaag tgatatctgt accttctact aatggaaaaa aacgtcttaa
     2641 tgataagtat gatgcaacga acatttatga agttaaggat gctagtttta agtattggaa
     2701 tcagcaaacg tatgtgttgt cggatattaa ttttaatgtt aatagaggcg aaaagattgc
     2761 gattgtgggt ccttctggtt caggaaaaag tacattacta caaattatgg cagggttata
     2821 tcaattagat agtggctctg ttcgtttcga aaatatggat atgtttgaaa tagatgacaa
     2881 agataagttt gaatcgttaa atgtcttgct acaatctcaa caattatttg atggtacaat
     2941 acgtcaaaat ttatttaccg atgaaaaaga tgaagcggtg caagcaatat ttaagcaatt
     3001 agatttagaa catttggcac tagaacgtca aattgactta gatggtcata cattatctgg
     3061 cggagaaatt cagcgtttag cgattacgag gatgttatta aaagatactg catcaacatg
     3121 gattttagat gaaccaacaa ctgcattaga taaacaaaat agtttaaaag ttatggattt
     3181 aattgaagca catgcagaaa cattaattgt tgctacacac gatttaactt tattgtcacg
     3241 ttttgagacc atcattgtga tgataaatgg taaaatagtt gaaaagggaa actatcaaca
     3301 attactagct aatcaaggtg ctttatggaa tatgattcaa tataatgcat aaaaaaactg
     3361 cttgataggc taagaaacct ttcaagcagt tttttaacta acaagcttac gcgtgctaat
     3421 tgtttttcta ttcttaataa attctaaaca ttactttaat tgtcatgaca aaagttaatt
     3481 atttttcctt tgtttcatca aatgcatgaa tgactttacc taataagcga ttaagttctt
     3541 taacttcatc ttgcgataaa gaagaagctg aagcgacttt gtcagatgca ttacttaatt
     3601 ctggtctaat agtttcactt ttgtcagtca agtgaataaa tacttcacgt tgatcgactt
     3661 cggaacgttc acgcttaatt aagtctactt gttccattcg ttttaataat ggtgatactg
     3721 taccagtatc gagtgctaat tcagttacga ctttcttgac gtttacagga gattcatccc
     3781 ataaaattgt taagacaaga aattgtgggt atgttagatt gtacttctta aaaactttgt
     3841 tagagtagta gcgattaact tgtctttgag cattgtacaa actaaagcat agctgttctt
     3901 ttaaattatg ttgatcagac attaaagttc tcctccagac atactatccg tttttttctc
     3961 ttttcggatt ggtaatcatt aaaaagttga ttgtttatta attcacaact ttctttgatt
     4021 caatgccatg ctaaaattaa agtatgttta aagtttagaa gatatttttg attaaatcaa
     4081 gcaaaaagat aatttaatat atatgttatc atttttaaaa ataactgtaa tagaaaagag
     4141 aatataaaat gaaaaataat aaagatgaaa aaataagaat atccataatt aacggatttt
     4201 tgggtagtgg taaaaccacg ttactgacac attatattag tgaattatta aaaaatgatg
     4261 agaaaattaa aatcatcatg aatgaattcg gtacttttga tattgatagc aatagtattt
     4321 caaatgaaat tgaagtccat tcattgatta atggttgtgt ttgttgcgat cttaaacaag
     4381 aacttgtcta tgaactaaaa gccattgctt taaaagggga cgttaatcat gtcatcatag
     4441 aagcgacagg cattgcgcat cctttggaat tactagttgc atgtcaagat ccgcaaatcg
     4501 ttaatttctt tgaaaagccg attatttatg gtgtattaga tgcgactcga tttttagaac
     4561 gtcatcaata taccgaaaat acagtttcgc tgatggaaga tcagttgaaa ctaagtgaca
     4621 tgattattat taataaaatt gatcttataa ctgatgacag tcttgagaaa attgataagc
     4681 aattaggtat gatttgtgca agtattccaa cttataaaac aacctatgga aaagtttcgt
     4741 tggaagaatt ggacttaact gttaaagaca gagagatatc gtctcatcat caccatcatc
     4801 atgggattaa aagtatgact tacacgttta caggtccgat tgatcgtcat ttgttttatc
     4861 aatttataat gaaattaccg gaatctgttc tacgtttgaa aggttatgtg tcatttagag
     4921 atcaaccaaa tgcaatttat gaatttcaat atgcatatgg tttaccagac tatggaataa
     4981 ttggcatgca attaccatta acgattgtta ttattggtga aactttagat acaaatcaca
     5041 tacgtaatca attggatatg ctacaattta cgtaaatcag aaaatgtgtt gtttcaattt
     5101 aacggaaata gcacattaat ttcttaatac ttttattgtg aatattttgt gacattgagg
     5161 gatggagtgg atgagatatg gaacaaataa tgattaatca ctatgttcat ttttctaggc
     5221 ttgtacaagg tttttggcgt gcaaatgaat ggaagatgac tgcgaaagag ttaaattatt
     5281 ttataaatga attagttgaa cgtggaatta caacgatgga tcatgctgat atttatggag
     5341 attatcaatg tgaatcactg tttggtaatg ctttggattt atcacccgaa ttaagaaata
     5401 aaattcaaat tgttacgaaa tgtggtatca ttttgccttc taagcaattt gattttacaa
     5461 atggacatcg ttatgatttg agtagtaagc acatcgtgaa atctgttgaa cagtcattaa
     5521 tcaatttgaa tgtagattat ttagatagtc tactcattca tcgtccttca ccattgatgg
     5581 atccagaaca agttgctgat gcattaacta aacttgttaa acaaggtaag ttgaagtcat
     5641 tcggggtgtc gaattttaat cattcacaat accaattgtt aaatcaatat attatgaaag
     5701 aaagactaca tattagcatc aatcaattag aattatcgcc atatcacgtt gatagtttac
     5761 aagatggaac aatggattca atgtatcaaa accatgttca aatcatggct tggagtcctt
     5821 ttgcaggcgg taaaattttc gacaaggaag atattaaagc gcaacgtatt atgaaagttg
     5881 ttcaatcaat agctgacaaa tatggtgtga gtgacacagc tgtgatgata gcgtggttag
     5941 taaaaatacc gcatcgtatc atgccgatac ttggaacaag tcagttaaag cgtattgatc
     6001 aagcaatcga agggctacaa cttaatttag atgatcagtc gtggtttgac atttacaccg
     6061 ctattatcgt acaagatatt ccgtaaactt atttactttt aaatcataaa ggagcatatc
     6121 atgacaaacg aagataaacg tttcgaacaa ttaagatttg aacgcaaatt tatagttatt
     6181 ccgtatttaa tttatgcagt cattgtatta ctattaaata ttttctattc tgatttgaaa
     6241 ataacaatga cattattcgg acttttcttt gcgtataatg tagtcatttt gttcatagca
     6301 tttgttaaac attataaacg cacattgtta ctaagtctta tattaacagt gcttagtggc
     6361 gcggcattct ttggaattat ttatgtttat ggcattaatc atttttaaac attaaaaaga
     6421 gactatctac aataatctca tttgcatttt atctaactaa gtttaacttt t
//
LOCUS       DAKTIP010000146         6470 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAKTIP010000146 DAKTIP010000000
VERSION     DAKTIP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.148"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(123..323)
                     /locus_tag="O0305_002086"
     CDS             complement(123..323)
                     /locus_tag="O0305_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275215.1"
                     /translation="MTNPTRTKRCGGDVLYGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            567..1052
                     /gene="tnpA"
                     /locus_tag="O0305_002087"
     CDS             567..1052
                     /gene="tnpA"
                     /locus_tag="O0305_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCY0275216.1"
                     /translation="MSSDTNSLAHTKWNCKYHIVFAPKYRRQVIYGKIKKDIGIILRQ
                     LCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGYLKGKSSLMIFDRHANLKYRYG
                     NRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQISMEEYLDPFTGEKNKKRKKK
                     E"
     gene            complement(1188..2780)
                     /gene="pckA"
                     /locus_tag="O0305_002088"
     CDS             complement(1188..2780)
                     /gene="pckA"
                     /locus_tag="O0305_002088"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCY0275217.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     regulatory      2935..3045
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3151..4344
                     /locus_tag="O0305_002089"
     CDS             3151..4344
                     /locus_tag="O0305_002089"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCY0275218.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     gene            4469..5377
                     /locus_tag="O0305_002090"
     CDS             4469..5377
                     /locus_tag="O0305_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCY0275219.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNLKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            5589..6422
                     /locus_tag="O0305_002091"
     CDS             5589..6422
                     /locus_tag="O0305_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0275220.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNNVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQRIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHAVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKKFE
                     G"
BASE COUNT         2237 a          980 c         1185 g         2068 t
ORIGIN      
        1 tttcacacat catttcaatg tgcgattttg aacttggaag taacatttca taatgtatga
       61 attcaaaagg agtatgtaaa caatataatg tttaaacctc atcactttta aatattctaa
      121 ttctaatgag aaatacaaat ttttttattt tctaaaatag cgtaatcatg cgttttattt
      181 actattctta aaaaatattc aaaaaaagtt ttagtttcta attgacaatg attctcacta
      241 atgtataata gtagttgaaa atgattatca ataccatata gaacatcccc cccacaacgt
      301 ttcgttcttg ttggattggt cattttcaaa tattcccctt ttatatgccc gtaaaagaca
      361 atatacgtta taacaacgtt ttataaaagc agtaaaccct tacgacactt taggtttact
      421 gcttttgttt tttgtaaaaa gaaaaccata cgctatgcgt atggttcaga aaaggttcta
      481 ccattgtcac aaaaaatgca tctctacgtg ctagaataaa tattggtcag ccaaccaaaa
      541 taatcaacac gaggagatgc tatttaatgt catctgacac aaacagttta gcacatacaa
      601 aatggaattg taagtatcat attgtctttg cacctaaata cagaagacaa gtgatatatg
      661 gaaaaataaa aaaagatata gggattatat tgcgtcaatt atgtgaaaga aaaggtgtag
      721 agataattga agcagaggca tgtaaagatc atatccatat gttagttagt ataccaccca
      781 aacttggggt atcatcattt gttggctatt taaaaggaaa aagtagttta atgatatttg
      841 atagacatgc taacttaaag tatagatatg gaaatagaaa gttttggtgt aaaggatttt
      901 atgtggatac agtaggtaga aataaaaaag tgattgaaaa ttatattcgt aatcaattac
      961 aagaggatat cgttgcagac caaatctcaa tggaagaata tttagatcct ttcactggag
     1021 agaaaaataa aaaaagaaag aaaaaagagt aaccctttag ggttgctgga atagtagtgc
     1081 agttggctga cttgtcagtg cccttttagg gctggccagt aaggaaggct tatagccgca
     1141 gaacaaacca cccgttcaca cgggtggttt tgatttagta ttcaaattta tttgttgaag
     1201 ctaccttttt cagcaatatg ttcaactttt tcaccaaatt tttcgaagtt cttttcaaaa
     1261 cgttgaatta aatcttctgc ttgtgcttta tatttctctt tgtcgctcca agcattaatt
     1321 ggatttaaaa ttgttttcgg tacatcttca atttctacag gaatgcttaa accaaacgta
     1381 ctatcttttg tatattctgc atttttcaat ttgccagaaa tcgcttggtt taccatttga
     1441 cgtgtgtaat gtaagctgat tctacgtcct acaccatatt ttccgccagt ccatccagta
     1501 ttaacaagat aaacatcaac atcatgtaaa tcgataagtt cacctaatag atcagcgtaa
     1561 acagtagggt gtaacgggaa gaacggtgct ccgaaacatg ttgagaatga tggttcaggt
     1621 tctgtcacac cacgctctgt accagctaat ttagaagtga aaccactcaa gaaatgatac
     1681 attgcttggt ctttatttaa ctttgaaatc ggtggaataa caccaaatgc atccgcagtt
     1741 aagaaaataa ttgtatttgg atgtgctgct ttagatggta ctacaatatt gtcaatgtga
     1801 ttaattggat aagcggcacg cgtgttttct gtataacgat tgtcttcaaa gtccactgaa
     1861 ccatcttctg caactacagt gttctctaaa attgcaccat atttgattgc gtcaaaaatc
     1921 tgtggttctt tttctttgga aagattaatt gcttttgcat agcagccacc ttcgatatta
     1981 aagaccccgt ttttattcca gccgtgttca tcatcaccga ttagtttacg gtgtgggtca
     2041 gccgataagg ttgttttacc agtgccagat agaccaaaga ataatgcaac atcgcctttt
     2101 tcaccaacgt ttgctgagca atgcatgctc ataatatctt gcatcggtaa gagataattc
     2161 attacagaga agataccttt tttcatttca ccagcgtatt cagtaccgcc gattaaaatg
     2221 actttgtgtt taaatgaaat aatgacaaag gtttcagatt tagtaccatc aacttctgga
     2281 tctgctttaa aatgtggtgc agaaacgata gtgaagttag gtttaatctt tgtagcttct
     2341 tcttttgatt caggtctaat aaacatattt ttagcaaata aattatgcca tgctaattca
     2401 ttgatgactg taagttttaa cattgtatct ttatcgctac cagcgtagcc tttaaatacg
     2461 tatagttcat cttttttatc taaatagtct agtactttat ggtataactt caagaaagtt
     2521 tcttcatcga taggttgatt aatttctccc caatcaatgt tatctctata tgaaggttca
     2581 gagacaaaaa atttgtcttt aggcgaacga ccagtatatt ttccagtact tgcattaaca
     2641 gcaccaagtt ctgttaatac cccttcgtta ttgtctaaga ttttattata aagttgtgtc
     2701 gtcgaaagtt gaaaatgtga cgttggtttt ttcagtaatt tgtcaatttt agttgtttca
     2761 gtgtatgtgt ctactgacat cctaaatccc tccaaagcga taatttaaat gtaagccttt
     2821 acatttaatt agtataacac attaacataa ttaatctaca atattttacc gtaaatttat
     2881 ttgtgaaaaa ttgacattaa ttcattaatt aggtactatt atttttaacg gattctctta
     2941 tcctgagtgg tggagggaca tggacccaat gaaacccagc aacctctttt ttataaaaga
     3001 aaggtgccaa accgtttgca gacaaatagg tctgaacgat aagagcgaat ggacgtatta
     3061 ggccttctct ctatattaat agttagaagg tcttttttat ttagctcaca gagagagaat
     3121 tttcgtaata taaatttaaa ggagcaaact atgttaaata acaaacgatt atttacttca
     3181 gagtctgtta cagaaggaca cccagataaa atcgctgacc aagtgtcaga tgcaatatta
     3241 gatgctattt taaaagacga tccaaatgca cgtgtagctt gtgaaacaac tgttacaaca
     3301 ggtatggcat tgatcgccgg cgaaatttct acaacaacat atgttgatat tccgaaagtt
     3361 gttagagaaa caattaaaga aattggatac acaagagcaa aatatggtta tgattatgaa
     3421 acaatggcaa ttttaactgc gattgatgaa caatcacctg acattgcaca aggcgtggat
     3481 aaagcattag agtatcgtga taaagatagc gaagaagaaa ttgaagcaac aggtgcaggt
     3541 gaccaaggtt taatgtttgg ttacgcaacg aatgaaacag agacgtatat gcctctagca
     3601 atttatttat cacatcaatt ggcaaaacgt ttatcagatg tgcgtaaaga tggtacgtta
     3661 aattatttac gaccagacgg taaagttcag gtcactgttg aatatgatga aaatgataac
     3721 ccagtacgta ttgatacgat tgtcgtatca acacaacatg ctgaggatgt gacacttgaa
     3781 caaattcaag aagacattaa agcacatgtc atttatccaa ctgttccaga aaacttgatt
     3841 aatgaacaaa ctaaattcta catcaatcct acaggacgct ttgtaatcgg tggacctcaa
     3901 ggtgatgcag gattaacagg acgtaaaatc attgttgata cgtacggtgg ctatgcacgt
     3961 catggtggcg gatgcttcag tggtaaggat cctacaaaag tagaccgttc agctgcatac
     4021 gctgcacgtt atgtggctaa aaatattgtt gcagcaggtt tagcagatca atgtgaagta
     4081 caattggcat atgcgattgg tgttgcagaa ccagtgtcaa ttgcaattga tacatttgga
     4141 acaggtaaag tttctgaagg gcaacttgtc gaagcagtta gaaaacactt tgacctaaga
     4201 cctgcaggta ttattaaaat gcttgatttg aaacaaccga tttacaaaca aactgctgct
     4261 tatggtcatt ttggacgtac agatgtctta ttcccatggg aaaaattaga caaagtggaa
     4321 gaattaaaag acgcagtaaa ataataagat gaattgaata tagctaaata aaagattttc
     4381 ggtatataaa tgataatatg tatcgaaaat ctttagcttt tctattataa ttaaagttat
     4441 tcatatatta aaaaggagcg ttgttgtgat ggatttatct tcaccgatag tcattggact
     4501 cattattgca gtagtcattg cattaatctt ttttgtcctt tttttagttg ccttaggtag
     4561 taagaaaaaa gttaagcgac aaacagaaga aaagtatgaa caacaagaac aaaacattaa
     4621 aaagtctcat gaagaagctt tagagaaaga gcgtattcaa aataaaaaga caattacaaa
     4681 acaacaagaa gattataatc acatggttag tacaaaagat cgtgagattg acgcattaaa
     4741 attgttctcg aaaaatcata gtgaatatgt aacggacatg agattaattg gtattcgtga
     4801 aagattagtt aaagaaaaac gaattcgacc agaagatatg cacattatgg caaatatctt
     4861 tttaccaaag gatggcttta ataatattga acgaattagt catttagtat taacaagaac
     4921 tggtctttat attattgatt ctcaattgtt aaaaggtcat gtttataatg gtattagtgg
     4981 cggtcaattt aaagatttac caccaatgga acaagtattt gatacattgg atttagataa
     5041 atctagaccg caaactattg tgatggatca aaatgatgac aaacgttcat tatcatttgt
     5101 aaattattca gatcaaattg aggcgattaa acaattggct gaagacttac aaaaagaatt
     5161 aggtgccaaa tatacaccaa cttcaatttt atactttaat cttaaaaatg aaggcgatgt
     5221 aacgatttca aattataatc aaaatagtgc tgtcaaagtg cttgtcggtg ctgaacaatt
     5281 agacgagttc tttaacaaat ttgtattcca tggtcgtatt caatacaacg tggaagactt
     5341 acaacaaatg atggataaaa ttgaatcatt caattaaatg ttaggctgaa gtgatgacta
     5401 tatgttgtta cttcggcttt tttagtttgt cataaattaa acagaattac ttttacaatt
     5461 tgattaatat ttgagagata ttactagaat tttagtggtt gattgctcta agtgtttttt
     5521 ctcatgtatt caactcagat ttgttatgat aacagggtat aatacatcaa aaaggtgagg
     5581 cgatatttat ggaggttaaa acattttata atggaaacac gatgccacaa attggtttag
     5641 ggacatttcg tgtggaaaat gatgaaaatt gcatggaaag tgttaagtat gccattgaac
     5701 aaggatatcg tagtattgat accgcaaaag tttatgggaa tgaagaacaa gtaggtgctg
     5761 gcattcgtgc cggattggag tcaactggta ttgctagaga agacttattt attacttcaa
     5821 aattatattt tgaggatttc ggtcgtgaaa atgtagcagc tgcttacgaa gctagtttat
     5881 ctagattagg tttaaaatac ttagatttgt atctagtaca ttggccaggt acgaacgaag
     5941 ccgtaatggt tgatacatgg aaaggtatgg aagatttata taaaaataat aatgttaaaa
     6001 atataggtgt cagcaatttt gaacctgaac atttggaagc tttgttggca caagtgtcaa
     6061 ttaaacctgt aattaatcaa gttgaatatc atccatattt aacccaacat aaattgaaat
     6121 tatatttggc agcacaacgt atcgtgatgg aatcttggtc accattgatg aatgcacaaa
     6181 ttttaaatga tgagacaatt aaagacattg ctcaagaatt aggaaagtca cctgcccaag
     6241 ttgttttaag atggaatgtg cagcatgctg tggttacaat ccctaaatcg gtgacaccaa
     6301 acagaatctc tgaaaatttc caaatatttg atttcgaatt atcagatgaa caaatgacgc
     6361 gaattgatgg tttaaatcaa gataagagaa ttggacctga tccaaaaaaa tttgaaggct
     6421 agattaaaat cgctcaactg atgaaaaggt tagatgaatt gtcagggctt
//
LOCUS       DAKTIP010000147         6374 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAKTIP010000147 DAKTIP010000000
VERSION     DAKTIP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.149"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            117..908
                     /gene="pgeF"
                     /locus_tag="O0305_002092"
     CDS             117..908
                     /gene="pgeF"
                     /locus_tag="O0305_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCY0275221.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            932..1600
                     /locus_tag="O0305_002093"
     CDS             932..1600
                     /locus_tag="O0305_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCY0275222.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            1597..2160
                     /locus_tag="O0305_002094"
     CDS             1597..2160
                     /locus_tag="O0305_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCY0275223.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            2172..2462
                     /locus_tag="O0305_002095"
     CDS             2172..2462
                     /locus_tag="O0305_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCY0275224.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            2572..3351
                     /locus_tag="O0305_002096"
     CDS             2572..3351
                     /locus_tag="O0305_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCY0275225.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            3375..3992
                     /locus_tag="O0305_002097"
     CDS             3375..3992
                     /locus_tag="O0305_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCY0275226.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            4213..>6374
                     /gene="ileS"
                     /locus_tag="O0305_002098"
     CDS             4213..>6374
                     /gene="ileS"
                     /locus_tag="O0305_002098"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCY0275227.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFY"
BASE COUNT         2358 a          913 c         1204 g         1899 t
ORIGIN      
        1 aagtcaaaaa ttatttgcgc aacattttaa ctttaaacat aaatgttata ttatataatt
       61 attaactttg tacagttaga cgaagataat ttaaatgaaa tgatggtgac gatcgagtga
      121 atgataattt taaaaagcaa ccgcatcatt taatatatga agagttatta caacaaggta
      181 ttactctagg tattacaact agaggagatg gtttaagtga ctatcctaaa aatgctttta
      241 atatggcgag atatattgat gatcgcccat ataatattac tcaacatcaa ttgcaattag
      301 ctgaagaaat tgcgtttgat agaaaaaatt gggtgtttcc cattcaaaca catgaaaata
      361 aagtcgcttg tattacaaag gatgatatag gcacaaatat agacacttta actgatgcgc
      421 ttcatggtat tgatgcgatg tacacatatg atagtaatgt cttattaacg atgtgttatg
      481 cagactgtgt accagtatat ttttatagta caaaacatca ttttattgca ttggcgcatg
      541 caggttggcg tggtacctat actgaaattg taaaagaagt gctaaaacat gtgaactttg
      601 atttgaaaga cttacatgtc gttattggac catctacatc atcaagttat gaaattaatg
      661 atgatattaa aaataaattt gaaacattgc caattgatag tgccaactat attgaaacta
      721 gaggacgaga tcgtcatggt attgatttga aaaaagccaa tgctgcatta ttaatttatt
      781 atggtgttcc taaagaaaat atttatacga cagcgtatgc tacatctgaa catttagaat
      841 tatttttctc ttatcgatta gaaaaaggtc aaacaggacg catgttagca ttcattggtc
      901 aacagtaaac aaggaggaga tatgtttgcg tgtgaaagat aatttacaac aaatctcaac
      961 acaaattaat gacaaaagtg aaaaaaataa tttttcaaca aaaccaaacg tgattgcagt
     1021 tacaaaatat gttacaatag agcgagctaa agaagcgtat gaggctggaa taagacattt
     1081 tggtgagaat agattggaag gcttttttca aaagaaagaa gcattaccat cagatgcggt
     1141 gatccatttt ataggatcat tacaatctcg aaaagttaag gacgttataa acgacgtaga
     1201 ttatttccat gctttagatc gattgagctt agccaaagaa attaacaaac gtgcagaaca
     1261 taaaattaaa tgtttcttgc aagtgaacgt ttcgggagaa gcttctaaac atggtattgc
     1321 tttagaagat gttgatcagt ttatagatga tcttaaaaaa tatgacaaaa tcgaaattgt
     1381 aggtttaatg acgatggcac cattgacaga tgatgaagca tatattagat cgttatttaa
     1441 acagttacgt ttgaaaaaag aagaaataca acgactcaat ttagaatatg cgccttgtga
     1501 tgaattatca atgggaatga gtaatgacta tcttattgca gttgaagaag gtgcgacgtt
     1561 tgttagaatt gggactaaac ttgtaggaga agaggagtga gccacttggc tttaaaagat
     1621 ttatttagtg gattttttgt aatagatgat gaagaggaag tagaagtacc tgacaaacaa
     1681 caacaggtaa atgaagcgcc agcaaaagag cagtcacaac aaacaacaaa acaaaacgca
     1741 atcaaatcag tccctcaaaa atctgcatca agatatacaa caacgtcaga agaaaggaat
     1801 aaccgtatgt ctaattattc aaaaaataat tcacgtaatg ttgtaactat gaacaatgct
     1861 acaccaaaca atgcatcaca agaaagttca aaaatgtgtt tattcgaacc acgtgttttt
     1921 tcagatacac aagatattgc tgatgagctt aaaaaccgcc gtgcgacact tgtcaattta
     1981 caacgtattg ataaagtatc agcgaaaaga attattgatt ttttaagcgg tactgtttat
     2041 gcaatcggtg gagatatcca acgtgtaggt actgatattt tcttatgtac gcctgataat
     2101 gtggaagtag ctggaagcat tacagaccat attgaaaata tggaacattc attcgactaa
     2161 ggagttaaca tatggatata aatgtgctag ctacaatatt taaattcatc ctttttgttg
     2221 ttgaaattta ttatttcggc atgattatat atttctttac atcttgggta ccaagtatta
     2281 gagaaactaa ggtaggttat tttttagcga aaatatatga acctttctta caaccattta
     2341 gaaaagtaat tccacctatt ggaattatcg acatatcatc aatcgctgca attatcgttt
     2401 tagtattatt ccaaaaaggg ttactccaaa tctttaattg gattttaatt caattacaat
     2461 aaattaagcg acataacgag gcaagggtac atgatacttt agcctcgttt ttgatatgca
     2521 tttttctgaa tataagggca atagatggta ttttataatt tttttaaggt agtgattaac
     2581 atagatattt atcaacactt tagacaggaa gaatacgaat taattgatca gctaacggat
     2641 aaatgtgatc aagcggaaca gcattatgca ccagtattaa cgcatttttt agatccaaga
     2701 gggcaatata tattggaagt gatttgtggc agttatgaag atttaaacgt atctttttat
     2761 ggtggaccta atgctgaaag aaaaagagca atcatttcgc cgaactatta tgaacctaaa
     2821 gaaagcgact ttgaattaac tttaatggaa atagattatc ctgaaaaatt cgtcacttta
     2881 aaacatcaac atattttagg gacattaatg tctttaggta tcgaacgcga acaagttgga
     2941 gatataattg tgaatgaacg aattcaattt gttttgacaa gtagattgga atcatttatt
     3001 atgttagaat tacaacgtat taaaggcgca tcagttaaac tttatactat tccagtaaca
     3061 gatatgatac aatctaatga gaattggaaa aatgaaagtg caacagttag ttctttaagg
     3121 ttagatgttg ttattaaaga aatgatacgt aaatcacgta cgattgcgaa acaactaatc
     3181 gaaaaaaaac gtgttaaagt gaatcacact attgttgatt cagcagattt tcaattacaa
     3241 gcaaatgatt taatatccat ccaaggtttt ggtagagcac acattactga cttaggtggt
     3301 aaaactaaaa aagataaaac gcacattacc tatagaacat tattcaaata gtaatgattt
     3361 aaggaggata acaaatgcct tttacaccaa atgaaattaa gaataaagag ttttcacgtg
     3421 taaagaatgg tttagaacct actgaagttg ctaatttttt ggagcaacta agcactgaaa
     3481 ttgaacgtct taaagaagat aaaaaacaac ttgaaaaagt aatcgaagag agagatacta
     3541 atattaagtc ttatcaagac gtgcatcaat ctgtaagtga tgctttgata caagctcaaa
     3601 aagctggtga agaaactaag caagctgcag agaaacaagc tgaagcgatt atagctaagg
     3661 cagaagcgca agctaatcaa atggttggtg acgcggtaga aaaagcacgc cgtttagcat
     3721 tccagactga agatatgaaa cgtcaatcaa aagtatttag atcgcgtttc cgtatgttag
     3781 ttgaagcgca attagactta ttaaaaaacg aagattggga ttacttgttg aattatgatt
     3841 tagacgctga acaagtgacg cttgaaaata ttcatcattt gcatgaaaat gatttaaagc
     3901 cagatgaagt tgcagcaaat gcacaaaata atgcatcaaa tacaccagac aataatcaac
     3961 aatccaatga ttcagaaaca actaagaagt aagaattaaa taaagacaga cgcgtaatat
     4021 acatttaact tttcacagcg aattaggtaa tggtgagagc ctagtaaaag catgtatgtt
     4081 atatcactgg ctttttaata tttaaataat gtaatgagag aactctaagt tgagttaata
     4141 agggtggtac cgcgagcaat cgtccctttt aatttaactt agagtttttt aaatttttaa
     4201 ggagtgaaaa aaatggatta caaagaaacg ttattaatgc ctaaaacaga tttcccaatg
     4261 cgaggtggtt taccaaacaa ggaaccgcaa attcaagaaa aatgggatgc agaagatcaa
     4321 taccataaag cgttagaaaa aaataaaggt aacgaaacat tcattttaca tgatggccca
     4381 ccatacgcga atggtaactt acatatggga catgccttga acaaaatttt aaaagacttt
     4441 attgtacgtt ataaaactat gcaagggttc tatgcaccat acgtaccagg ttgggataca
     4501 catggtttac caattgaaca agcattaacg aaaaaaggtg ttgaccgaaa gaaaatgtca
     4561 acagctgaat tccgtgagaa atgtaaagaa tttgctttag aacaaattga attacagaaa
     4621 aaagatttta gacgtttagg tgttcgtggt gactttaatg atccatatat tacattaaaa
     4681 cctgaatacg aagctgcaca aattcgtatt tttggagaaa tggcagataa aggtttaatt
     4741 tataaaggta aaaagccagt ttattggtct ccttcaagtg agtcttcatt agcagaagca
     4801 gaaattgaat atcacgataa acgttcagca tcaatttacg ttgcatttga cgttaaagat
     4861 gacaaaggtg tcgttgatgc agatgctaaa tttattatct ggacaacaac gccatggaca
     4921 attccatcaa atgttgcgat taccgttcat cctgaattaa aatatggtca atacaatgta
     4981 aatggcgaaa aatatattat tgcagaagcc ttgtctgacg ctgtagcaga agcactggat
     5041 tgggataaag catcaatcaa attagaaaaa gaatacacag gtaaagaatt agagtatgtt
     5101 gtagcacaac atccattctt agacagagaa tcgttagtga ttaatggtga tcatgttact
     5161 acagatgctg gtacaggttg tgtacataca gcaccaggtc acggggaaga tgactatatt
     5221 gttggtcaaa aatatgaatt gccagtaatt agtccaatcg atgataaagg tgtatttact
     5281 gaagaaggcg gccaatttga agggatgttc tatgataaag ctaataaagc cgttactgat
     5341 ttattaacag aaaaaggtgc actattaaaa ttagacttta ttacacatag ctatccacac
     5401 gactggagaa caaaaaaacc tgtaatcttc cgtgctacac cacaatggtt tgcctcaatc
     5461 agtaaagtaa gacaagatat tttagatgca atcgaaaata caaacttcaa agtaaattgg
     5521 ggtaaaacac gtatttacaa tatggttcgt gaccgtggcg aatgggttat ttctcgtcaa
     5581 cgtgtgtggg gtgtaccgtt accagtattt tatgctgaaa atggcgaaat tatcatgacg
     5641 aaagaaacag tgaatcatgt tgctgattta tttgcagaac acggttcaaa tatttggttt
     5701 gaaagagaag cgaaagactt actaccagaa ggatttacac atccaggcag ccctaacggt
     5761 acatttacta aagaaacaga cattatggac gtttggtttg attctggttc atcacaccgt
     5821 ggcgtgttgg aaacaagacc ggaattaagt ttcccagcgg atatgtattt agaaggtagt
     5881 gaccaatatc gtggttggtt caactcttct atcacaactt cagttgctac aagaggagta
     5941 tcaccttata aattcttact ttctcatggt tttgttatgg acggtgaagg taagaaaatg
     6001 agtaaatctt taggtaatgt gattgtacct gaccaagtgg ttaaacaaaa aggtgctgat
     6061 attgcgagac tttgggtaag tagtacggac tatttagctg atgttagaat ttctgatgaa
     6121 attttaaaac aaacatctga tgtttatcgt aaaatcagaa atacattaag atttatgtta
     6181 ggtaacatta acgatttcaa tcctgacaca gatagcattc ctgaatcaga gttattagaa
     6241 gtggatcgtt acttgctaaa tcgtttacgt gaatttactg caagtacgat taacaactat
     6301 gaaaactttg actacttaaa tatttatcaa gaagttcaaa actttatcaa tgttgagtta
     6361 agtaatttct attt
//
LOCUS       DAKTIP010000148         6291 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAKTIP010000148 DAKTIP010000000
VERSION     DAKTIP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.150"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1242)
                     /locus_tag="O0305_002099"
     CDS             complement(<1..1242)
                     /locus_tag="O0305_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275228.1"
                     /translation="MTDNYSLHIYKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDL
                     KRNYVTIIMLNDLYQIDGNDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSAERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKL"
     gene            1852..2655
                     /locus_tag="O0305_002100"
     CDS             1852..2655
                     /locus_tag="O0305_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0275229.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYN
                     NYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYT
                     SGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQ
                     GAYGHVAYVESVNSNGSVRVSEMNYVYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(2672..2914)
                     /locus_tag="O0305_002101"
     CDS             complement(2672..2914)
                     /locus_tag="O0305_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275230.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(3088..4488)
                     /gene="nhaC"
                     /locus_tag="O0305_002102"
     CDS             complement(3088..4488)
                     /gene="nhaC"
                     /locus_tag="O0305_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCY0275231.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(4583..5665)
                     /locus_tag="O0305_002103"
     CDS             complement(4583..5665)
                     /locus_tag="O0305_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCY0275232.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            5913..>6291
                     /locus_tag="O0305_002104"
     CDS             5913..>6291
                     /locus_tag="O0305_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCY0275233.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYY"
BASE COUNT         2151 a         1147 c          838 g         2155 t
ORIGIN      
        1 cagtttttga attgcagtag tatttaagtc gttaattaaa aatgcaatct tacatctatt
       61 actctcaaaa gtcatattaa aaattgtagc tactagcttt tctatcttgc ctctttcagc
      121 actcgttaac aattccttat attcatgttt aatacgaaac actacattta cattgtaatt
      181 cactatcttt tgaagtaata aagtgatttg ttcaatcgat agctcattgc ttgtgtcgat
      241 tgtaacaatt atatgcaagt tttctggatt aacacctaat ctttgaatga tttgtttaat
      301 agtataataa tccatccaat aaaaattact tcctttaata taaatgtttt taggttgata
      361 catttgactc ctttttgtag gctcaaaagg tatatcaatc tcgcgcatac ttgaagaact
      421 ttgattagta tcatcaaata attcaattat atttttatca atttcaacta attgggaatg
      481 gttaattgaa tgagatgttg gactatatct ttttctaatt aatttaggag tgttaccata
      541 cgtttcttta aagaggtgta taaaacgaga ataatgatta aaaccatgac tacttgcgat
      601 ttcttcaata ggcttcttag aagttaaaat atcaattaag caatgctcca gtctaatatg
      661 atttaaatat tgaataaaat tactataagg tgtcgcttta aacatgtcac ttaaagcttt
      721 gtttgtaata ctaacttgat taatgacatc tttcctattt atctttttat ggtggttgtt
      781 tgttaaataa tcgtgcactt cttcggctac taaatgacga ctaccatcgt atgtatttaa
      841 tgacattaat tcaacacaca tgtaattaat aatcttatca ttagcattat aagactgttg
      901 tttaattttg ctgtagatta aatacttaat caagattcta aaagtgctag caacctcatt
      961 tgttaatatc ttgccacgca tcatatagtt tgcattcata aacttcaaaa atgtctttgc
     1021 acttacttca acaacacaac atacactatc atcattacca tcaatttgat acaaatcatt
     1081 caacataata atggtgacat aatttctttt tagatcatat tgttgtagat tgattgaaac
     1141 tacacctgta ccttgtaacc aatatataat ttttaagttc gcttccttca cactactcat
     1201 tttcatactt ttataaatat gtagtgaata attatctgtc atcttacatc ctcacatata
     1261 caaatatatt gatttactcc tgtttgtctt tctcaaatca attgtcaaca tctaatattc
     1321 actaccaatt atatcgccac atgtttttta gcagtaacaa aacataaatc gattatatca
     1381 aataatactt ttattaaaac atttttttat gctaaattag gatattttat ccattaaata
     1441 tacattttca tccaaatacc aaagctttca taatcataga aacttgatcg ttcgtgtcga
     1501 atttttatgc attttctgat taaaagtcct atattatgca caaaatatac atttagaatt
     1561 ttcagtaata ttacaaattt gtaacagact tattttcgaa acattcagtt ttcacaaaga
     1621 atgccgtagt tataagtttt gcaaaatgca aatttctata cttttaaaat tctattgtgt
     1681 caagatatta gcaaaaaatt acaaaacgtt aacaaagcac acaaggacgc taatttctgt
     1741 tatagtcttt cttgtcgttt aattacgaga gataacttat aacattaatt ttgtcacacg
     1801 gacattacga aaatatttta gatttaaaat ttttaggagg atatttttaa aatgaagaaa
     1861 atcgctacag ctactatcgc aactgcagga ttcgctacaa tcgcaattgc atcaggaaat
     1921 caagctcatg cttctgagca agataactac ggttataatc caaacgaccc aacatcatat
     1981 agctatactt acactattga tgcacaaggt aactaccatt acacatggaa aggtaactgg
     2041 catccaagtc aattaaacca agataatggc tactacagct attactacta caatggttac
     2101 aataactata acaattacaa caacggttat agctacaata actacagccg ttacaacaac
     2161 tactcaaata ataatcaatc atataactac aataactata atagttacaa cacaaacagc
     2221 taccgtactg gtggtttagg tgcaagctac agcacttcaa gcaacaatgt tcaagtaact
     2281 acaactatgg ctccatcatc aaatggccgt tcaatctcaa gtggttatac ttcaggacgt
     2341 aacttataca cttctggtca atgtacatac tacgtatttg atcgtgtagg tggtaaaatc
     2401 ggttcaactt ggggcaatgc aagtaactgg gctaacgcag ctgcaagagc tggttacaca
     2461 gtgaacaata caccaaaagc tggtgcaatt atgcaaacaa ctcaaggtgc atacggtcac
     2521 gttgcatacg ttgaaagtgt taacagcaat ggttcagtaa gagtttcaga aatgaactat
     2581 gtttatggcc caggtgttgt aacttcacgt acaatctcag ctagccaagc tgctggttat
     2641 aacttcattc actaattagt gatggctatg tttacgccaa caaggtttca agagccttgt
     2701 tggctttttt aatttctatg aaaaaaatcg aattggtaaa aatctcaaaa tttcgatttt
     2761 caccaatccg attcactctg taataaagaa taaacaacat acagccattg acacacccaa
     2821 atttaggcat gtcaaatcgg ctatacattg ataaattttt agttaaagta cttacaaata
     2881 tacttgcctt caaattctca attaatttag tcattacaaa tcatgacata aaacttacat
     2941 tacatatatc aacatgttcc gctacaaaac atattcagta tatgtataga ggatatttat
     3001 tctaagttaa aaccttttca aattataacc gctacaatta taaatgatta aagtgtttca
     3061 acatattata tatattccac gctcacatta agttaaacgt gaattcgatg actttttaat
     3121 acctatccct gtaaaaccat agataattgc tataattgcg cataaataac atggtactgt
     3181 ccatatgaaa aattcttcaa cagagacatg aagttgattc gtatagtaaa tacctgatgt
     3241 tccccatggt attaaaggta agaccatcgt acttgagtct tcaagtgttc ttgataaaac
     3301 ggaacgagag acttggtatt tttcgaacaa atctttcatt aacacaccga ccataataat
     3361 tacaatcgaa gcaacacctg cagcgaatac aagcgcaata caacaaataa cagtaataca
     3421 tattaaactt cctacagaat ggatgccttt agaaatagta gttaataaga cttctaaaca
     3481 tcctgctttt tctacaatac ctgcaaatgc atatccgcaa aatatcgtta ctaatatttg
     3541 ggtcatactc atcataccac cctgttctaa caagcttttc acactggatg aaatatgaga
     3601 ctgatgtatc attgattcgt taaaaccact aaatgttgct ttgaaaccat ctgtcatttt
     3661 gaaatgatga ttaaaagtcc ccactataat ggcagaaaag cttgatatta gcatagctgg
     3721 cactgtagcc attttaaata gcaaacaaac aatgatcaca attaagggaa cccagaccca
     3781 tatgttaatt tgataaatct gtgcaagctc tgataacaaa gtttgaatct gtttatcatt
     3841 tgaatgccct ttaaattgaa atccagcaat aaaccatact aataaaccta tgattgatgc
     3901 aggtatcgtc gtccacatca tcgaatgtat atgtttaaat atattaactt tagtaacaag
     3961 cgccgctaaa ttagttgtat ctgataatgg tgacatttta tcgccaaaca cagcccctgc
     4021 tataatagca cccgctgcca tccctggagg aatccccaat tgattaccaa tagaaataag
     4081 tgcaatccct gcagttgatg cagagcccca tgctgtacca gttgctacag atgtaacagc
     4141 acttataaaa aaggctgata ttaaaaaata gcttggattc aataaatcta agccataata
     4201 aatcaaggct ggcactgtgc cagaaaacat ccaactgcct actataattc ctaccgctag
     4261 tataattaaa attgcaggca ttgcagtatt taaacgttct gcaatgcctt tttctaaatc
     4321 ttgccatgtt aagcctacac gttttgcaat ccatgtggca tatgctgagg caataattaa
     4381 taatacttga attggaatat caaaaaacac aaagcctgtt acaacaacaa taaccattac
     4441 aatcattgtc gaaattgatt ctaaaaaggt gggttttctt ttcatcatct atcgtcccct
     4501 tcgcttattg atgagaaacc tcttttcttc aatttattgc ttttgcataa tgctatatcc
     4561 ttttctatct tcaagccatc atttatttta aatatttttc taaatcaagc ttatctaaac
     4621 caatttcttc aactgttaag ccttcctcaa agaagtctct ctctaaaatg gttgatgcaa
     4681 ttacaattac tgcatctata tttggtgtcg gtacatcaat aacacgacct aagcttgacc
     4741 atagtactaa accatatgca atatcttcag ttaaatatct gctttctaca tgatttggtc
     4801 ctggaatttg tgaaaatact gggcttgtat taaacaaacg atttaatggt tcatcttctt
     4861 tatcacgttc taaataacca cgttcaatac gtgattcttt agctgttgat aattcaaaac
     4921 ctaatctacg gcctaaattc aaacgttcta attcgattgc atgaagtaat ctaactgtat
     4981 gtttagtaat tccttcttta tataaagcga actcgccagc ataatcaatg cgaccgacat
     5041 tcaataatgt tggtcctgga tgcacttcag gattaccatt ttcaagattt gttttaatta
     5101 agctttcctc ttttactaaa tgatcataaa tacttgaaac tttgtcataa caatcattta
     5161 gacagcttct atcatatgtt gaaaagaaga tacgacgtac atttagagat aaatcaactg
     5221 ctgcattttc aaaatcgaca cgcgtaccat acgtcaacgt attagcttcc gctagttgtg
     5281 gttttgtttc aatatgtcta tcttctaaaa cattcataaa acgaattgac cccattgctg
     5341 cagccatgtt gaagaatatc aactgattat cagttacatg ctctgccatt acatcagcat
     5401 aatactctat gtatgaagat ggaataatca cttgaacaat ttcagcatct tttaaaacat
     5461 attccatatc atcactaata tcagtgaatt ttacgaaacg ttcatcacct tcattattaa
     5521 aatcaaatcc gcccttttcg attgcgtttt gaaacttact tatagattga ttacgacaat
     5581 ataatttaac atcgtggcct ttgctcacca tatctactgc tgccgtaact gcgccatttc
     5641 ctgatcctac aattgctatt ttcataaaca accctctttc tatctttggt taaaattaaa
     5701 aacaaattat acgtaagaca atataaaaat gatgcgaatt cattacgcat cattataaat
     5761 gttaagtata tataacctta aattaaccta tttaaacgcc gacgtccacc aattattttt
     5821 atttttaata attgtttaat aaacatgttt atttttgatt atattttgtt atagttaaag
     5881 ataatattac aattttcaaa ggagtgtcta atatgacaac tgaaatgaac gacgtaacaa
     5941 atcatcaacc gaatcgcaaa tccaatgatg agaacattat ggcgatgctt atctatttat
     6001 taagtttatt cacatctatt attggtccac tgataatatg gttattgaaa aaagatgaat
     6061 cgaaactagt tgatcgtgct ggaaaaaatt atcttaacta tacgatttct tatattattt
     6121 ggtctatcgt tttagtcgtt atcactttaa taggcgtttt cctaattgct acagatattg
     6181 atttcattat tattataggt ttcatcatta cgtttatcgg tattttatcc atattcgctt
     6241 tctcaatatt atcttttgta ttcacaatta ttgcgtgtgt caaatattat a
//
LOCUS       DAKTIP010000149         6276 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAKTIP010000149 DAKTIP010000000
VERSION     DAKTIP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.151"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            185..1501
                     /gene="rho"
                     /locus_tag="O0305_002105"
     CDS             185..1501
                     /gene="rho"
                     /locus_tag="O0305_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCY0275234.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            1619..1873
                     /locus_tag="O0305_002106"
     CDS             1619..1873
                     /locus_tag="O0305_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCY0275235.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            2220..2819
                     /locus_tag="O0305_002107"
     CDS             2220..2819
                     /locus_tag="O0305_002107"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCY0275236.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            2820..3896
                     /gene="prfA"
                     /locus_tag="O0305_002108"
     CDS             2820..3896
                     /gene="prfA"
                     /locus_tag="O0305_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCY0275237.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            3883..4719
                     /gene="prmC"
                     /locus_tag="O0305_002109"
     CDS             3883..4719
                     /gene="prmC"
                     /locus_tag="O0305_002109"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCY0275238.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            4803..5849
                     /locus_tag="O0305_002110"
     CDS             4803..5849
                     /locus_tag="O0305_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCY0275239.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            5846..6265
                     /locus_tag="O0305_002111"
     CDS             5846..6265
                     /locus_tag="O0305_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCY0275240.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
BASE COUNT         2261 a          927 c         1236 g         1852 t
ORIGIN      
        1 ctgttttaat tttgcaattt ttataaatag attttgtaat taaaataaaa atttgctata
       61 gttattcatg tatttaaaag gttggggatt agcataatgg gattgtgcta gcacagttat
      121 ttatgcattg tcatgcctat ctattactta ctaactaaaa aataatgaaa tgggtgtaaa
      181 ctatatgcct gaaagagaac gtacatctcc tcagtatgaa tcattccacg aattgtacaa
      241 gaactatact accaaggaac tcactcaaaa agctaaaact cttaagttga cgaactatag
      301 taaattaaat aaaaaagaac ttgttctagc tattatggaa gcacaaatgg aaaaagatgg
      361 taactattat atggaaggta tcttagatga tatacaacca gatggttatg gttttttaag
      421 aacagtgaac tattctaaag gggaaaaaga tatttatata tctgctagcc aaattcgtcg
      481 ttttgaaatt aaacgtgggg ataaagtaac tgggaaagtt agaaaaccta aagataacga
      541 aaaatattat ggcttattac aagttgactt tgtcaatgac cataacgcag aagaagtgaa
      601 gaaacgtccg catttccaag ctttgacacc actttatcca gatgagcgta ttaaattaga
      661 gacagaaaca caaaattatt caacgcgcat catggattta gtaacaccga ttggtttagg
      721 tcaacgtggt ttaatagtgg cgccacctaa agcaggtaaa acatcgttat taaaagaaat
      781 agcgaatgca atcagtacga acaaaccaga tgcaaagcta tttattttgt tagttggcga
      841 gcgtcctgaa gaggtaacag atttagaacg ctcagtagaa gctgctgaag tcgttcattc
      901 aacgtttgac gaaccaccag aacaccatgt taaagtagct gaattattac ttgaacgtgc
      961 aaagcgttta gtagaaattg gggaagatgt cattatttta atggattcta taacgagatt
     1021 agcacgcgct tataacttag ttattccacc aagtggtcgt acattatcag gtggtttaga
     1081 tcctgcatct ttacacaaac caaaagcatt cttcggtgca gcgagaaata ttgaagcggg
     1141 tggaagttta acaatacttg caactgcatt agttgatacg ggttcacgta tggacgatat
     1201 gatttacgaa gaatttaaag gaacaggtaa catggagtta catttagatc gtaaattgtc
     1261 tgaacgtcgt atcttccctg caattgatat tggcagaagt tcaacgcgta aagaagaatt
     1321 gttgataagt aaatctgaat tagacacatt atggcaatta agaaatctat tcactgactc
     1381 aactgacttt actgaaagat ttattcgcaa acttaaaagg tctaagaata atgaagattt
     1441 cttcaagcag ctacaaaagt ctgcagaaga aagtactaaa acgggtcgac ctataattta
     1501 ataaacatta tataggggct tgcgttttga attaattacc tttataatta cacagtattg
     1561 ggtaaaaact cacaaataac tctgttccag atggttcagg gcaaaggagc tgaaaataat
     1621 gaaacaagga attcatcctg agtatcacca agtaatcttt ttagatacta ctacaaactt
     1681 caaattctta agcggttcta caaaaacatc ttcagaaatg atggagtggg aagatggaaa
     1741 agaataccca gttattcgtt tagatatttc atctgattca catccattct acactggacg
     1801 tcaaaaattc gctgctgcgg atggacgtgt ggaacgtttc aacaaaaagt ttggtcttaa
     1861 atcaaacaac taatatagta atcgttaagc tgtctgcatc ctataatgga attgtagaca
     1921 gcttttttat ttataagcgt agtaagggta tatacttacg actgtgttca aagaagtgac
     1981 tcaataccat gttgttaata cataatgata aatttccccc tttattaaag tttgtattga
     2041 gtagaaacat cgcacgaata agaagcaaca aattagaata gctgtatata aatatgcaag
     2101 acaaatttat aaaataattg ttcatgtaca atatcgatat aattatgaaa aagacattta
     2161 tgaatatttg aaatatgata taataaagcg attatgtttt gaaaaaggtg gaactctcaa
     2221 tgtatgaaac ttaccattcc ggttggatag aatgtattac aggtagtatg tttagcggta
     2281 aatccgaaga actaataaga cgtctaagaa ggggtatata cgctaaacaa aaagtcgttg
     2341 tatttaaacc ggcgattgat gatcgatatc ataaagaaaa agtggtctct cataatggta
     2401 atgccattga agcgattaat atttctaaag caagtgaaat tatgacgcac gacttaacta
     2461 atgttgatgt tataggaata gacgaagtgc aattttttga cgatgaaatt gtaagtatag
     2521 tagaaaaact atcagcagat ggacaccgtg ttattgttgc tggattagat atggatttta
     2581 ggggcgaacc gttcgaacca atgcctaaat tgatggctgt cagtgaacaa gtaacgaaac
     2641 ttcaagcagt ttgtgctgtt tgtggttctt catctagtag aacgcaacga ttaataaatg
     2701 gtaaaccagc taaaatcgat gatccgatta tcttagtagg tgccaatgaa agttatgagc
     2761 cacgttgtcg cgcgcaccat atcgtagcac ctagtgataa taataaggag gaattataag
     2821 tgtttgatca attagatatt gtagaagaaa gatacgaaca gttaaatgaa ctgttaagtg
     2881 acccagatgt tgtaaatgat tcagataaat tacgtaaata ttctaaagag caagctgatt
     2941 tacaaaaaac tgtagatgtt tatcgtaact ataaagctaa aaaagaagaa ttagctgata
     3001 ttgaagaaat gttaagtgag actgatgata aagaagaagt agaaatgtta aaagaggaga
     3061 gtaatggtat taaagctgaa cttccaaatc ttgaagaaga gcttaaaata ttattgattc
     3121 ctaaagatcc taatgatgac aaagacgtta ttgtagaaat aagagcagca gcaggtggtg
     3181 atgaggctgc gatttttgct ggtgatttaa tgcgtatgta ttcaaagtat gctgaatcac
     3241 aaggattcaa aactgaaata gtagaagcgt ctgaaagtga ccatggtggt tacaaagaaa
     3301 ttagtttctc agtttctggt aatggcgcgt atagtaaatt gaaatttgaa aatggtgcgc
     3361 accgcgttca acgtgtgcct gaaacagaat caggtggacg tattcatact tcaacagcta
     3421 cagtggcagt tttaccagaa gttgaagatg tagaaattga aattagaaat gaagatttaa
     3481 aaatcgacac gtatcgttca agtggtgcag gtggtcagca cgtaaacaca actgactctg
     3541 cagtacgtat tacccattta ccaactggtg tcattgcaac atcttctgag aagtctcaaa
     3601 ttcaaaaccg tgaaaaagca atgaaagtgt taaaagcacg tttatacgat atgaaagttc
     3661 aagaagaaca acaaaagtat gcgtcacaac gtaaatcagc agtcggtact ggtgatcgtt
     3721 cagaacgtat tcgaacttat aattatccac aaagccgtgt aacagaccat cgtataggtc
     3781 taacgcttca aaaattaggg caaattatgg aaggccattt agaagaaatt atagatgcac
     3841 tgactttatc agagcagaca gataaattga aagaacttaa taatggtgaa ttataaagaa
     3901 aagttagatg aagcaattca tttaacacaa caaaaagggt ttgaacaaac acgagctgaa
     3961 tggttaatgt tagatgtatt tcaatggacg cgtacggact ttgtagtcca catgcatgat
     4021 gatatgccga aagcgatgat tatgaagttc gacttagcat tacaacgtat gttattagga
     4081 gagcctatac agtatatagt tggctttgcc tcattttatg gtagaacgtt tgatgtaaac
     4141 tcaaattgtt tgataccaag acctgaaact gaagaagtaa tgttgcattt cttacaacag
     4201 ttagaagatg atgcaacaat cgtagatatc ggaacgggta gtggtgtact tgcaattact
     4261 ttgaaatgtg aaaagccgga tttaaatgtt attgctactg atatttcact tgaagcaatg
     4321 aatatggctc gtaataatgc tgagaagcat caatcacaaa tacaattttt aacaggggat
     4381 gcattaaagc ccttaattaa tgaaggtatc aagttgaacg gcttgatatc taatccacca
     4441 tatatagatg aaaaagatat ggttacgatg tctccaacgg ttacgagatt cgaaccacat
     4501 caggcattgt ttgcagataa ccatggatat gctatttatg aatcaatcat tgaagattta
     4561 cctcacgtta tggaaaaagg cagcccagtt gtttttgaaa ttggttacaa tcaaggtgag
     4621 gcacttaaat caataatttt aaataaattt cctgacaaaa aaatcgacat tattaaagat
     4681 ataaatggcc acgatcgaat cgtctcattt aaatggtaat tagaagttat gcctttgcta
     4741 tgattagtta agtgcatagc tttttgcttt atattatgat aaataagaaa ggcgtgatta
     4801 agttggatac taaaatttgg gatgttagag aatataatga agatttacag caatatccta
     4861 aaattaatga aataaaagac attgttttaa acggtggttt aataggttta ccaactgaaa
     4921 cagtttatgg acttgcagca aatgcgacag atgaagaagc tgtagctaaa atatatgaag
     4981 ctaaaggccg tccatctgac aatccgctta ttgttcatat acacagtaaa ggtcaattaa
     5041 aagattttac atatactttg gatccacgcg tagaaaagtt aatgcaggca ttctggccgg
     5101 gccctatttc gtttatattg ccgttaaagc taggctatct atgtcgaaaa gtttctggag
     5161 gtttatcatc agttgctgtt agaatgccaa gccattctgt aggtagacaa ttattacaaa
     5221 tcataaatga acctctagct gctccaagtg ctaatttaag tggtagacct tcaccaacaa
     5281 ctttcaatca tgtatatcaa gatttgaatg gccgtatcga tggtattgtt caagctgaac
     5341 aaagtgaaga aggattagaa agtacggttt tagattgcac atcttttcct tataaaattg
     5401 caagacctgg ttctataaca gcagcaatga ttacagaaat acttccgaat agtatcgccc
     5461 atgctgatta taatgatact gaacagccaa ttgcaccagg tatgaagtat aagcattact
     5521 cacccaatac accacttaca attattacag atattgagag caaaattgga aatgacggta
     5581 aagattggtc ttctatagct tttattgtgc cgagtaataa ggtggcgttt ataccaagtg
     5641 aagcgcaatt cattcaatta tgtcaggatg acaatgatgt taaacaagca agtcataatc
     5701 tttatgatgt gttacattca cttgatgaaa atgaaaatat ttcagcggcg tatatatacg
     5761 gctttgagct gaatgataat acagaagcaa ttatgaatcg catgttaaaa gctgcaggta
     5821 atcacattat taaaggatgt gaactatgaa gattttattc gtttgtacag gtaacacatg
     5881 tcgtagccca ttagcggaaa gtattgcaaa agaggttatg ccaaatcatc aatttgaatc
     5941 aagaggtata ttcgctgtga acaatcaagg tgtttcgaat tatgttgaag acttagttga
     6001 agaacatcat ttagctgaaa cgaccttatc gcaacaattt actgaagcag atttgaaagc
     6061 agatattatt ttgacgatgt cgtattcgca caaagaatta atagaggcac actttggttt
     6121 gcaaaatcat gttttcacat tgcatgaata tgtaaaagaa gcaggagaag ttatagatcc
     6181 atacggtgga acaaaagaaa tgtatgtaca tacctatgaa gaacttgtaa gtttaatttt
     6241 aaaattaaaa gatattattt gctagtagga attctg
//
LOCUS       DAKTIP010000150         6269 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAKTIP010000150 DAKTIP010000000
VERSION     DAKTIP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.152"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            72..1028
                     /locus_tag="O0305_002112"
     CDS             72..1028
                     /locus_tag="O0305_002112"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCY0275241.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1228..1707
                     /locus_tag="O0305_002113"
     CDS             1228..1707
                     /locus_tag="O0305_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0275242.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1722..2561
                     /gene="fakB2"
                     /locus_tag="O0305_002114"
     CDS             1722..2561
                     /gene="fakB2"
                     /locus_tag="O0305_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCY0275243.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2647..3180
                     /gene="msrA"
                     /locus_tag="O0305_002115"
     CDS             2647..3180
                     /gene="msrA"
                     /locus_tag="O0305_002115"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCY0275244.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3173..3601
                     /gene="msrB"
                     /locus_tag="O0305_002116"
     CDS             3173..3601
                     /gene="msrB"
                     /locus_tag="O0305_002116"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCY0275245.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3613..4113
                     /locus_tag="O0305_002117"
     CDS             3613..4113
                     /locus_tag="O0305_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCY0275246.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4113..4334
                     /locus_tag="O0305_002118"
     CDS             4113..4334
                     /locus_tag="O0305_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCY0275247.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4526..6016
                     /locus_tag="O0305_002119"
     CDS             4526..6016
                     /locus_tag="O0305_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCY0275248.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDKALENQV
                     KAFQQANKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT         2358 a          880 c         1118 g         1913 t
ORIGIN      
        1 gctacataaa gttaagttat atcaaactaa atatacatac tataaataat gaaaatgagg
       61 tgttatcgca tatgttgaat tcatttgatg cagcatatca cagtctttgt gaagaagttt
      121 tagaaatagg aaatacacga aatgatcgca caaatacagg tacgatttcg aaatttggtc
      181 atcaacttcg ctttgactta tctaaaggat ttccactatt aacgacaaag aaagtttctt
      241 ttaaattagt agcaaccgaa ttattatggt tcattaaagg agatacaaac atccaatact
      301 tattaaaata taataataat atatggaacg aatgggcttt tgaaaattat atcaaatcag
      361 acgagtataa aggtccagat atgacagatt tcgggcatcg tgcattgagt gatcctgaat
      421 ttaacgaaca atataaagaa caaatgaaac aatttaagca acgtattctt gaagatgata
      481 catttgcgaa gcaattcggg gatttaggaa atgtttatgg taaacaatgg cgagattggg
      541 ttgataaaga tggtaatcat tttgatcaac ttaaaacagt aattgaacaa attaagcata
      601 atccagattc aaggcgacac atcgtatctg catggaatcc aacagaaatt gatacaatgg
      661 cacttccgcc ttgtcatacc atgttccagt tttatgtcca agatggtaag ttaagttgcc
      721 agttatacca acgtagcgca gatatctttt taggtgtgcc atttaatatc gcaagctacg
      781 ctttattgac acaccttatt gccaaagaat gtggacttga agtgggtgaa tttgtgcata
      841 catttggaga tgcacatatt tattcaaatc atattgatgc gattcaaaca caattagcac
      901 gtgaaagctt caatcctcca acattaaaaa ttaacagtga caagtctatt ttcgacataa
      961 attatgaaga tttggaaatt gttgactatg aatcacatcc agcaataaaa gctccaatag
     1021 cagtgtagtc attgcatagt tagctaacca tatagacatc aaaatgacat catagtattt
     1081 tcaagtgcaa aaaagtactt ttttgtgtta aacgttttca taaattatgc aaaatcatta
     1141 tttctatcac actttatgat aaaaattgtg ttaaattaaa gataacttag taataaaaaa
     1201 tgaaatgata gaagaaggag gataattatg actttatcca ttctagttgc acatgacttg
     1261 caacgagtaa ttggttttga aaatcaatta ccttggcacc taccaaatga tttgaagcat
     1321 gttaaaaaat tatcaacagg tcatacttta gtaatgggtc gtaagacatt tgaatcgatt
     1381 ggtaaaccac taccgaatcg tcgaaatgtt gtacttactt cagatacaag tttcaacgta
     1441 gagggcgttg atgtaattca ctctattgaa gatatttacc aactaccggg ccatgttttc
     1501 atatttggag ggcaaacatt atttgaagaa atgattgata aagtggacga catgtatatt
     1561 actgttattg aaggtaaatt ccgtggtgat acgttctttc caccttatac atttgaagac
     1621 tgggaagttg cctcttcagt tgaaggtaaa ctagatgaga aaaatacaat tccacatacc
     1681 tttctacatt taattcgtaa aaaataaggg ggaaaacgac catgacaaaa cagattatag
     1741 taacagactc aacatccgat ttatctaaag aatacttaga agcaaacaac attcatgtaa
     1801 ttcctttaag tttaactatt gaaggagctt catacgttga ccaagtagat attacatcag
     1861 aagaatttat taatcatatt gaaaatgatg aagatgtaaa gacaagtcag ccagccatag
     1921 gtgaatttat atctgcttat gaagaactag gaaaagatgg ctctgaaatc ataagtattc
     1981 atctttcttc aggattaagt ggtacatata acactgctta ccaagcaagt caaatggtag
     2041 atgctaatgt aactgttatt gattcaaaat ctatttcttt tggtttaggg tatcaaatac
     2101 aacacctagt agagcttgta aaagaaggtg tctcaacttc tgaaatagtt aaaaagttaa
     2161 atcatttaag agaaaacatt aaattatttg tagttatagg gcaattgaat caattaatta
     2221 aaggtggcag aattagtaaa acaaaaggtt tgattggtaa tcttatgaaa attaaaccaa
     2281 ttggtacact agatgatggt cgcttagagc ttgtgcacaa tgcgagaact caaaattcta
     2341 gtatccaata cttgaaaaag gaaattgctg aatttatagg agatcatgaa atcaaatcca
     2401 ttggtgtcgc acatgctaac gtcattgaat atgttgataa attgaagaaa gtttttaatg
     2461 aagcttttca tgtgaataat tacgatataa atgtaactac accagttatt tctgcacata
     2521 ctggtcaagg tgcgattggc ctcgtagtcc ttaagaagta aatttaatct tttcagtgtt
     2581 aattacttcc atttcaatcc tttatagact aaatttataa ttagatagat agaggaggta
     2641 attcatatga caaaagaata tgcaacatta gcaggaggat gtttctggtg catggttaaa
     2701 ccatttacat catatccagg catcaagtca gtcgtatctg gttatagtgg cggtcatgtt
     2761 gacaacccaa cttatgaaca ggtatgtacg aatcaaaccg gccatgtcga agcagtacaa
     2821 attacgtttg atccagaggt tacttccttt gaaaatatat tagacatata tttcaaaaca
     2881 tttgacccaa ctgatgatca agggcaattt ttcgatagag gcgaaagcta tcaaccagtc
     2941 attttctatc atgatgaaca tcagaaaaag gctgctgagt ttaaaaagca acaattaaat
     3001 gaacaaggta ttttcaagaa accagtgatt acacctatta aaccatataa aaatttctat
     3061 ccagctgaag actaccatca agattattac aaaaagaacc cggtacatta ttaccaatat
     3121 caacgtggtt caggtagaaa agcgtttata gaatcacatt gggggaatca aaatgcttaa
     3181 aaaagataaa agtgaactaa cagatataga atatattgtt acacaagaaa acggcactga
     3241 accaccattt atgaatgaat attggaatca ttttgctaaa ggaatttatg tagataaaat
     3301 ttctggtaaa cctttattta catctgaaga aaagtttcat tctgaatgtg gatggcctag
     3361 cttttccaaa gcgcttgatg acgatgaaat tatagaatta gtcgacaaat catttggtat
     3421 gttgagaact gaagtgcgtt cagaagaatc aaatagtcat ttaggacatg tctttaatga
     3481 tggacctaaa gaaagtggcg gcttaagata ctgtatcaat tccgctgcaa ttcaatttat
     3541 tccatatgaa aagttagaag aattgggtta tggcgattta atatcacatt ttgataagta
     3601 ggagtggaaa gtatgtttaa aaaattattc ggaaaaggca aagaagttca gaaagatatc
     3661 gcaatttatg caccattaac tggagaattc gtgaaaattg aagatattcc agatcctgta
     3721 ttcgcacaaa aaatgatggg cgaaggtttt ggtattaatc caactgaagg agaagttgtg
     3781 tctccaattg caggacgtgt tgacaatgtc tttccaacta agcatgcgat tgggttaaaa
     3841 gcagataacg gattagaatt attagttcat atcggtttag acacagttca attagacggt
     3901 gaaggatttg aagtgttagt gtctagtggt gacgaagtta acgtcggaga tccattagta
     3961 agattcaacc ttgaatatat taataataac gctaaatctg taatttcacc aattataatt
     4021 actaacactg atcaagcagc ttcaattaat atttatgatg aaaatgctgt gattaaaggt
     4081 gaaacaaaag tgattgatgt gacaatgaac taatgaaaaa ctattcgttt tatcaatttg
     4141 tcatgacagt tcgtggtcga cacgacgata aaggtcgtct agcagaagag atatttgacg
     4201 atcttgcttt cccaaaacac gatgatgatt ttaacatact gtctgattat attgagacac
     4261 atggtgattt cacattgcca atgtctgtat ttgatgattt atatgaagaa tatacggaat
     4321 ggctaaaatt ttaatataat ttttaataat aatagttaga accagggtga tgcaattcgt
     4381 tatcctggtt ttaatttaaa ataaactaag ttgtgactaa aaattaatca attatagtga
     4441 aatatggtgc gctatcttgc ataaattgat atgattaact acacagaatt taaaagtaca
     4501 taatacataa taaggaagtg atacaatgga tgataagcaa cacacatctt catccgatga
     4561 tgaacgcgct gaaattgcaa caagcaatca agaccaagaa actaattcat cgaaacgcgt
     4621 tcacttaaaa cgttggcaat tcatatcaat attaattggt acaatcctaa taacagctgt
     4681 catcacagtt gttgcatata tttttataaa tcaaaaaata agtggtttaa acaaaactga
     4741 tcaagcaaac ttaaataaaa ttgaaaatgt gtataaaatc ttaaatagtg attattacaa
     4801 aaaacaggac tctgacaagt taagtaaagc tgcaattgat ggcatggtca aagaattaaa
     4861 agatccttat tctgaatatt taacaaaaga acaaacgaaa tcctttaatg aaggtgtttc
     4921 aggtgatttt gtaggtattg gtgcagaaat gcaaaagaaa aatgatcaaa ttatggttac
     4981 tagtcctatg aagggatctc cagcagaacg tgctggcatt cgtcctaaag atgtcattac
     5041 taaagtaaat ggaaaatcaa ttaaaggtaa agcattagat gaagttgtca aagatgttcg
     5101 tggtaaagaa aacactgaag tcactttaac tgttcaacga ggtagtgaag aaaaagacgt
     5161 taagattaaa cgtgaaaaaa ttcatgttaa aagtgttgag tataagaaaa aaggtaaagt
     5221 tggagttatt actattaata aattccagaa tgatacatca ggtgaattga aagatgcagt
     5281 tctaaaagct cacaaagatg gtttgaaaaa gattgtttta gatttaagaa ataatccagg
     5341 tggactacta gatgaagctg ttaaaatggc aaatattttt atcgataaag gaaaaactgt
     5401 tgttaaacta gaaaaaggta aagatactga agcaattcaa acttctaatg atgcgttaaa
     5461 agaagcgaaa gacatggata tatccatctt agtgaatgaa ggttcagcta gcgcttctga
     5521 agtgtttact ggtgcgctaa aagactataa taaagctaaa gtttatgggt caaaaacatt
     5581 cggcaaaggt gtcgtacaaa ctacaagaga gtttaaggat ggttcattgt taaaatatac
     5641 tgaaatgaaa tggttaacgc cagatggtca ttatattcac ggtaaaggca ttaaaccaga
     5701 cgttactatt gacacaccta aatatcaatc tttaaatgtc attcctaata cgaaaacatt
     5761 taaagttgga gacgatgata aaaatattaa aactattaaa attggtttat cagctttagg
     5821 ttataaagtt gataatgaat caacgcaatt tgataaagct ttagaaaatc aagttaaagc
     5881 tttccaacaa gcgaataaac ttgaggtaac tggtgaattt aataaagaaa cgaataataa
     5941 atttactgag ttattagttg aaaaagctaa taaacatgat gatgttctcg ataagttgat
     6001 taatatttta aaataagcga tacacactac taaaattgta ttattattat gttaatgaca
     6061 cgcctcctaa atttgcaaag atagcaattt aggaggcgtg tttattttta ttgacgtcta
     6121 actctaaaag atataaatta gacatttaca aatgatgtaa ataacgcaat ttctatcatc
     6181 gctgataaca attcatggtt taatatgcaa tgagcatata ctttttaaat agtattattc
     6241 actagtttta acaatcaatt aattggtat
//
LOCUS       DAKTIP010000151         6261 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAKTIP010000151 DAKTIP010000000
VERSION     DAKTIP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.153"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(367..1347)
                     /gene="czrB"
                     /locus_tag="O0305_002120"
     CDS             complement(367..1347)
                     /gene="czrB"
                     /locus_tag="O0305_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCY0275249.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(1349..1669)
                     /gene="czrA"
                     /locus_tag="O0305_002121"
     CDS             complement(1349..1669)
                     /gene="czrA"
                     /locus_tag="O0305_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCY0275250.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            1821..2486
                     /locus_tag="O0305_002122"
     CDS             1821..2486
                     /locus_tag="O0305_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0275251.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGDQDIKDALTQFEN
                     ETD"
     gene            2921..3859
                     /locus_tag="O0305_002123"
     CDS             2921..3859
                     /locus_tag="O0305_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCY0275252.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            3890..4120
                     /locus_tag="O0305_002124"
     CDS             3890..4120
                     /locus_tag="O0305_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275253.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            4792..6165
                     /locus_tag="O0305_002125"
     CDS             4792..6165
                     /locus_tag="O0305_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCY0275254.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
BASE COUNT         2255 a          858 c         1152 g         1996 t
ORIGIN      
        1 tatacaactg caattacagc aaatgctata agtgcatata tcaatgttat taataattgt
       61 ggtttagcat ttttaaatgc tgatttaaag taagtaaaca aagtggtgcc tccttttctt
      121 catataaata caataacaaa aaacacgtta ttttcatatt aatatctaat aaaaattaat
      181 taaagtgact aaagttagag aattataata gtaaattgtc aaaaatatga ctaatgtaag
      241 aaataatagt tgaaaatttg ttaataaaca ttgcatcatc aaagaccaaa caagtcgcca
      301 taaagacata aaaaacctac tgccattgaa aagtttaatc gatgcaatgg cagtaggtta
      361 aaattattaa tgtacatgcg cgtgatgatg agcatgatgg ttatgtgaat gactatgtgt
      421 tcctgaacat ataatcgatt catcatgttt gtgattaggc gtttctaatt gaatagtcat
      481 atggtgaata tttaaatgta ataaatcatg ctcaatgttt tctaataata attcacattc
      541 tttcattgtc aatgtatggt ctacaacaac atgacaactc aatgcattca tatcatttga
      601 aattgtccaa acatggcaat catgcacact ttgtattcgt gaatcctttt taatagtagt
      661 tataacttca tctatatcaa catcacttgg tgtgccttcc attaaaatgt taattgaaga
      721 ttttgtgata ccccaagcac tttttaaaat aataacggaa actaaaatac ttgcgatagg
      781 atcggcgatt gtccatccaa atgcccaaat taaaatagct gcagtaatgg cgccaactga
      841 acctaataag tctccgataa catgtagaaa agcaccacgc atatttaaat tgtgtgaagt
      901 gtcgccgcct ttaaacataa agaatgcaac aacgatattg acaattaaac cgataatact
      961 aatgattaac atttcttttg attgaacttc agaaggaaca aagaaacgtt taatcgcttc
     1021 aaaaacaatc aaaatactta ttacaaaaag cgttacaccg ttaaataacg ctgcgagtac
     1081 ttcgaaacgt ttataaccaa atgtttttgt agttgtggca ttcttttcag cataaataaa
     1141 tgcgacaagt gcaacaccta atgagaatgt gtcgctaaac atatggatac cgtcagatag
     1201 taatgccaag ctgttagcaa ggagaccgcc gatgatttcg ataaacatat atagaccgat
     1261 tattaaaaac gatataaaca atactttctt attatttgta gttacatgac tatgcatatg
     1321 gtcatgatga tgatgtgaat gagacatatt ataacccact ttctttagga tgattcgcgt
     1381 gatgtatggc ttgctttaac atagttgcta cgtggatgtc atctaatgaa taaatcattg
     1441 attggccttg tcgttttgct ttcacaagat gcacactttt aagtaatttt aattggtgcg
     1501 agacatttga ttgagataaa ttcaattgat gtgaaatgtg accaacactt gcttcgctga
     1561 ctgataacaa ttccatgata cgtattcgat tgtaatcgcc taatgccttg aaaatttcag
     1621 ttacgcgttc taatgtatct gtatttattt ctgaatattg ttctgacatt atatgacaat
     1681 cctttcatgt gaatatttgt tcatatatta atttaatacg ttcccagtag tatgtcaatt
     1741 gatttttata gttgtaacaa gttttacata tctcataaag tggtatggca tagagaataa
     1801 taaataggag agtgttgtaa atgaatattt tggttatagg tgctaatggc ggtgtaggtt
     1861 cgttactagt gcaacaatta gcaaaagaaa atgtaccatt tactgctggt gttaggcaat
     1921 cagatcaact taatgcgtta aaatcacaag gtatgaaagc aattcttgtt gatgttgaaa
     1981 atgattcaat agagacttta actgagacgt ttaaaccatt cgataaagtt atcttttcag
     2041 taggttctgg agggaacacg ggtgcagata aaacaattat tgtcgattta gatggtgctg
     2101 ttaaatcaat gattgcgagt aaagaggcca atattaaaca ctatgtcatg gtttcaacat
     2161 atgattcgag acgtcaggct tttgatgata gcggtgattt aaagccatat acaatagcta
     2221 aacattacgc tgatgactat ttaagacgtt caggtttgaa ttatacaatt ttacatccag
     2281 gggcacttac aaatgcagca ggatctggaa agatagaagc tgcacaatat tttgatggta
     2341 aaggtgaaat tcctagagaa gatgttgcga cagtattaaa agaaattgtc acatccaatc
     2401 attttaatca ccaagagttt caaattatta gtggtgatca ggacattaaa gatgctttaa
     2461 ctcaatttga aaatgaaacg gattaataag gatagtaaat atgatttgaa gcgggttact
     2521 ttttcgtaac tcgttatttt ttgaaaatga tgaattataa gtatgaataa gtacgtattg
     2581 tctatgcgtt ttacaaagaa tttgaacaat gtatggcaca actattaaag ttgtttatta
     2641 attggtataa tgggcgagta aatttaaagg gaaatatgta caaatgtaag agagatataa
     2701 tctctatgct ataaattttt gaaaatgtta aagtactgta aatttaagtg aagcgcttta
     2761 attggcagta ctgcaatgtt agtaaatatg gtacaatcac taatagttaa tttgaaatgt
     2821 ggaagttaat aatacgatgt aaatgagaac aaaatcaaaa tataatggaa caacaacatc
     2881 agagttctat tgcttactta atcacaatgg aggtgcaaca atgccattat ttttacaacc
     2941 aattttaaaa acaaaattat ggggcggtca acgtctaagt gagtttggat atcaattaga
     3001 caatgataca actggggaat gttggtgtgt gtcagcacat ccaaatggta cgagcgagat
     3061 tattaatgga ccatatcaag gtcaaacatt agaccgtatt tggtcagaac atcgtgaatt
     3121 gtttggtgat ttcccaagca aagattttcc gcttctaact aaaatagtgg atgcaagaga
     3181 atcactttct attcatgtgc accctgataa ttcttatgct tatgagcatg aaaacgggca
     3241 atatggcaaa tctgaatgtt ggtatattat agatgcagaa gaagatgcag aaatagttat
     3301 agggacatta gcagagtcta gagaagaagt tgcgaatcat gttcaacacg gaacgataga
     3361 gtcgatactt agatatatta aagtaaaacc tggagaattc tattttattc cagcaggaac
     3421 agtacatact atttcttcag gaatattagc atacgaaacg atgcaatcgt cagacattac
     3481 atatagactt tatgatttca atcgtcaaga taatcaatat aatgatagac cgttaaatat
     3541 tgaaaaagct ttagacgtta ttcagtacaa tgcaccatta cctaatattt tgcctgaaag
     3601 cgaaattatt gaaaaccata agtgtacaca cattgtatcg aatgatttct ttacattggt
     3661 taaatgggaa atttctggca cgttaaatta tatgaagcct agagagttct gtttagttac
     3721 agtgttggaa ggcgaagggc aaatgattgt cgatggtgaa attttcaaac tgactactgg
     3781 tacaaacttt attttgactt ctgaagattt ggatagtgtc tttgaaggtg atttcacatt
     3841 gatgattagc tatgtgtaat agtgtaataa caatttagga gtgaatgatt tgaagcaatt
     3901 tctatatatt gcgttagtat gtggtgtgat agcaggtctt ggtgctttct tacatatacc
     3961 gcagtatccg agcatgacaa ttccacgtat agtagctatt ttaggaatta tcagtgctat
     4021 gttgactttt aaagacaagc aaatcagcgc ctcattaaag tttagcgcat tgttaattaa
     4081 tgtgctgcca ttatgcggta cctttgtagc ttcaaattaa ttgtcgatag cgcgtttgaa
     4141 tataatacag tgcattaaca gaagaaatat gattgtgtta atgacattga tgaaaataaa
     4201 gcacatcaac ataacctctc acttgcatag tagtgagggg gtttttgttg gtttatataa
     4261 aatgaagtaa ttaatattta atgaaaatta agacatgata aaattctaac ggcagtatag
     4321 atatttaaaa atgtgtatca atggcgctat agagataagc aagcatagtg gtatcatata
     4381 taaatttgta atttttattg catagtgtat gcaagcatgt tttttatatg caaaataggt
     4441 agctaaatat gttaaactgt ttgtaacaat ttttatcatt aagacagata taataaataa
     4501 aatatagaaa actgattaaa aggtatacag ttgctcgtat gtgtgtccaa ctgtatttag
     4561 agtcgcactt gttgtaaagc aaaatagttc gacttactta gaagtgaaaa cgataagggt
     4621 ctgggacgta aattaatgtc ctgggcgcta caatgttata ggaaatgcga ttaaaatcgc
     4681 agtgaacaaa gggtatacag ttgttcgcat acgcgtccaa ctgcataaga aacactaaaa
     4741 gaatgtaatt catagtgttt ctaaaattta ttcaggttaa ggagtattaa gatgacagca
     4801 gaaacgaatt atttttggtt gaattgtgga tataaccgtt ggaatcataa tgaaccgtta
     4861 gtaggacaaa cagctttatt tgaatcaggc gcacatttta acccatctca aggatttaga
     4921 gcgtttaaaa aggctaaagt aggagatcaa gtcatctttt atcaagttca gacggatact
     4981 ggactacttg gatgtggtga aattattagt gttgaaacag gtgcacaaaa taaaatacgt
     5041 gtacagtttc gttttaatga acaattgaaa ccattaacag cagattattt aaagcgtagt
     5101 gaagctttgg aatttcgtat aagtaatatg aaagagacat tgttcaatca aatcactgca
     5161 gaagagtttg atttaataag tggtcttggt aaaggcgaaa ttaagatacc gcgttatttc
     5221 tttttagctg aaactgaaga atttgaacct ggaaaccaat atacgattta tacgcacacg
     5281 tacaacggta ttaaacgtaa tggttatcat ttttatactc agttagaaga aggcgacaat
     5341 attatttttt ataatcgaac gaaaaatcaa tctgtcgttg gaattggaga agtatcaaag
     5401 catattcatg aaaaaccacc aattcctggt agaacaaata gtacagtgat tgaagtgtct
     5461 tatgagaaag acattacacc gattacatta agtacattaa ataagcatcc taaattgaaa
     5521 aacttatatt tccttcaaga aaatgcaaaa caggctattg caagtatgtc acaagcacag
     5581 tatgatgcaa ttattgaaat gagcgataat aatggattga aatctccatt tgaaatggtt
     5641 caaaaaccag atatgcttga aagtgaaaaa gaagaagcgt taaagccatt tattttactt
     5701 gttgtagatc gaaaagaaga aggcttaaaa gcggctaatg acttattgca aaaagcgaat
     5761 gcaaatcctg ttataacaac aggtcatcct gatttttcag aagatatgct ttatggcaag
     5821 tatttaccaa atgaaacagg ggcattatac tatcgtgaag ggtttattac tcaattaatg
     5881 cctaaaaagg ataaaagtta tttagttatt gataatttta atcgtataga tacagacatt
     5941 ttccaaacgt atattaatgt gttagaaggc tatgaagtaa cattaccacg ttataataaa
     6001 gacggtaata tgattaaatg gtcacgacaa aaagattctt tctattattt caatcctaat
     6061 tggcatattg taggtattac atatgacagt ttagaggaaa ttaaggagaa atattcagag
     6121 cagtttttaa aatacacacg tatagtcaaa gtcaaacatg actaatgata tatggtagtt
     6181 tttgaaaaga agtaaggata ggattaaaaa ttgcataatt gcatatttga ataaccgcat
     6241 aaagaaaaaa tgacgggtaa t
//
LOCUS       DAKTIP010000152         6258 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAKTIP010000152 DAKTIP010000000
VERSION     DAKTIP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.154"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            263..2512
                     /gene="pflB"
                     /locus_tag="O0305_002126"
     CDS             263..2512
                     /gene="pflB"
                     /locus_tag="O0305_002126"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCY0275255.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            2535..3290
                     /gene="pflA"
                     /locus_tag="O0305_002127"
     CDS             2535..3290
                     /gene="pflA"
                     /locus_tag="O0305_002127"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCY0275256.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            3626..5389
                     /locus_tag="O0305_002128"
     CDS             3626..5389
                     /locus_tag="O0305_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCY0275257.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDIVGLKMRQNGHEAKFVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHLENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(5553..5897)
                     /locus_tag="O0305_002129"
     CDS             complement(5553..5897)
                     /locus_tag="O0305_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCY0275258.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKQRLESIY
                     NSISNPLHSQNN"
     gene            6087..>6258
                     /locus_tag="O0305_002130"
     CDS             6087..>6258
                     /locus_tag="O0305_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275259.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GTLIDSRYLNSAL"
BASE COUNT         2178 a          939 c         1157 g         1984 t
ORIGIN      
        1 tcatttttta taaacaaata tatactccta aattaacttt taaagcaatg aaaatagtga
       61 acattataac tgttgtgtaa cagaatgcaa ttagcatatt actgttacac aaattagtac
      121 agtttctatg ttttgacata catttgatga aaattgtaca taatttatgt gaaaaaaatc
      181 acaacaaaca tgctacaatg actatgaaaa cgttaacata gcatttcaaa ttcacaacat
      241 tatacagatg gaggcgttta gtatgttaga aacaaataaa aatcatgcaa cagcttggca
      301 aggatttaaa aatggaagat ggaacagaca cgtagatgta agagagttta tccaattaaa
      361 ctacactctt tatgaaggta atgattcatt tttagcagga ccaacagaag caacttctaa
      421 actttgggaa caagtaatgc agttatcgaa agaagaacgt gaacgtggcg gcatgtggga
      481 tatggacacg aaagtagctt caacaatcac atctcatgat gctggttatt tagacaaaga
      541 tttagaaaca attgtaggtg tacaaactga aaagccattc aaacgttcaa tgcaaccatt
      601 cggtggtatt cgtatggcga aagcagcttg tgaagcttac ggttacgaat tagacgaaga
      661 aactgaaaaa atctttacag attatcgtaa aacacataac caaggtgtat tcgatgcata
      721 ttctagagaa atgttgaact gccgtaaagc aggtgtaatc actggtttac ctgatgcata
      781 cggacgtgga cgtattatcg gtgactatcg tcgtgtagct ttatatggtg tagatttctt
      841 aatggaagaa aaaatgcacg acttcaacac gatgtctaca gaaatgtcag aagatgtaat
      901 tcgtttacgt gaagaattat cagaacaata tcgtgcatta aaagaattaa aagaacttgg
      961 acaaaaatat ggtttcgatt taagccgtcc agcagaaaac ttcaaagaag cagttcaatg
     1021 gttatactta gcataccttg ctgcaattaa agaacaaaac ggtgcagcaa tgagtttagg
     1081 tcgtacatca acattcttag atatctatgc tgaacgtgac cttaaagcag gcgttattac
     1141 tgaaagcgaa gttcaagaaa ttattgacca cttcatcatg aaattacgta ttgttaaatt
     1201 tgctcgtaca cctgattaca atgaattatt ctctggagac ccaacttggg taactgaatc
     1261 tatcggtggt gtaggtattg acggacgtcc acttgttacg aaaaactcat tccgtttctt
     1321 acactcatta gataacttag gtccagctcc agaaccaaac ttaacagtat tatggtcagt
     1381 acgtttacct gacaacttca aaacatactg tgcaaaaatg agtattaaaa caagttctat
     1441 ccaatatgaa aatgatgaca ttatgcgtga aagctatggc gatgactatg gtatcgcatg
     1501 ttgtgtatca gcgatgacaa ttggtaaaca aatgcaattc ttcggtgcac gtgcgaactt
     1561 agctaaaaca ttactttacg ctatcaatgg tggtaaagat gaaaaatctg gtgcacaagt
     1621 tggtccaaac ttcgaaggta ttaacagcga agtattagaa tatgacgaag tattcaagaa
     1681 atttgatcaa atgatggatt ggctagcagg tgtttacatt aactcattaa atgttattca
     1741 ctacatgcac gataaataca gctatgaacg tattgaaatg gcattacatg atacagaaat
     1801 tgtacgtaca atggcaacag gtatcgctgg tttatcagta gcagctgact cattatctgc
     1861 aattaaatat gcacaagtta aaccaattcg taacgaagaa ggtcttgtag tagactttga
     1921 aatcgaaggc gacttcccta aatacggtaa caatgacgac cgtgtagatg atattgcagt
     1981 tgatttagta gaacgcttca tgactaaatt acgtagtcat aaaacatatc gtgattcaga
     2041 acatacaatg agtgtattaa caattacttc aaacgttgta tacggtaaga aaactggtaa
     2101 cacaccagac ggacgtaaag ctggcgaacc atttgctcca ggtgcaaacc caatgcatgg
     2161 ccgtgaccaa aaaggtgcat tatcttcatt aagttctgta gctaagatcc cttacgattg
     2221 ctgtaaagat ggtatttcaa atacattcag tatcgtacca aaatcattag gtaaagaacc
     2281 agaagatcaa aaccgtaact taactagtat gttagatggt tacgcaatgc aatgtggtca
     2341 ccacttaaat attaacgtat ttaaccgtga aacattaata gatgcaatgg aacatccaga
     2401 agaatatcca cagttaacaa tccgtgtatc tggttacgct gttaacttca ttaaattaac
     2461 acgtgaacaa caattagatg taatttctcg tacattccat gaaagtatgt aacaaaattt
     2521 aaggtgggag cactatgctt aagggacact tacattctgt cgaaagttta ggtactgtcg
     2581 atggaccggg attaagatat atattattta cacaaggatg cttacttaga tgcttgtatt
     2641 gccacaatcc agatacttgg aaaattagtg agccatcaag agaagtcaca gttgatgaaa
     2701 tggtgaatga aatattacca tacaaaccat actttgatgc atcgggtggc ggtgtaacag
     2761 tcagtggtgg cgaaccattg ttacaaatgc cattcttaga aaaattattt gcagaattaa
     2821 aagaaaatgg tgtgcacact tgcttagaca catcggctgg atgtgctaat gatacaaaag
     2881 catttcaaag gcattttgaa gaattacaaa aacatacaga cttgatatta ttagatataa
     2941 aacatattga taatgacaaa catattagat tgacaggaaa gcctaataca cacatcctta
     3001 acttcgcgcg caaactgtca gatatgaaac aacctgtatg gattcgacat gtccttgtgc
     3061 ctggttattc tgatgataaa gacgatttaa ttaaactagg ggaatttatt aattctcttg
     3121 ataacgtcga aaagtttgaa attctgccat atcatcagtt aggtgttcat aagtggaaaa
     3181 cattgggcat tgcatatgaa ttagaagatg tcgaagcgcc cgatgatgaa gctgttaaag
     3241 cagcctaccg ttatgttaac ttcaaaggga aaattcccgt tgaattataa atacaattca
     3301 gaccgaaaag aaagcatatg caacttcaag agtgaagggg catatgcttc tttttcaatt
     3361 gagtattgag tattagcaag acgtagtaag tatatgagac aacttctaca atggttgaag
     3421 gaagacgttt ttgtaagtag ctatgctgat aaagaatgtg atgtcttgtt aaaggtgggg
     3481 ttccaatatc atcatttagc tgatgttgaa tgggttatta tttgctactt gcatatgaat
     3541 atgagtcttt tcaaattttt attgaccctg agtaatgaaa aatattaaga tgaaacttaa
     3601 tattaaagca atgcggagcg tgattatgaa gagaattagt aaagatatat gggcagtatt
     3661 taaattactg tatcaaaata aagggcgttt tagcattaat gccttactat tgcagttaat
     3721 catgattttt attagtagta catacttaat tttactattt aatatgatgt taaaagtagc
     3781 tgggcaaagc caacttacga ttaacaattg gacggaaatc gttagtcatc ccgccagtgt
     3841 gatacttctt attatattca tattaagtgt tgcctttctg atttatgtag agttttcatt
     3901 gttagtttat atggtttatg ccggctttga tcgacagatt attacattta aatccatttt
     3961 taaaaatgcc tttgtaaatg tgcgtaaact cataggtgta ccagttattt tctttgtcat
     4021 ttatttaatg ttaatgatac ccattgccaa cctaggacta agttcagtat taacaaaaaa
     4081 tatttacata cctaaatttt taacggaaga acttatgaaa acgacgaaag gtataatcat
     4141 ttacggtacc tttatgattg ctgtatttat attaaatttt aaattaatat ttactctacc
     4201 gttaacgatt ttaaaccgcc agtcgttatt taaaaatatg agactaagtt ggcaaattac
     4261 gaagcgaaat aagtttcggc ttgttataga aatagttata ttagaactca tcattggtgc
     4321 gattttaaca ttaattattt caggagcaac atatcttgct atttgtgtag atgaagaagg
     4381 agataagttt ttagtctcat caattttatt tgttgtattg aaaagcgcat tgttcttcta
     4441 ttatttattt acgaaattat cattaatcag tgtgttagta ctgcacttaa aacaagagaa
     4501 tgtattagac caaccgggct tagaatttaa atatccaaaa ccgaaacgga agtctaggtt
     4561 ctttataatt tcaatggtgc ttgcagtgac atgttttatc ggttataaca tgtacttact
     4621 ttacaataat actatcaata caaatatctc cattattggc catcgtggtt tcgaagataa
     4681 aggcgttgaa aattctattc cgtcattgaa agctgctgca aaagcgaatg tcgaatacgt
     4741 tgagttagat acaattatga cgaaagataa acaatttgtt gttagtcatg ataacaattt
     4801 gaaacgttta acaggtgtta ataaaaatat ttctgaatct aatttcaaag atatcgtcgg
     4861 tttgaaaatg cgtcaaaatg gacatgaagc aaaatttgta tccttagacg aatttattga
     4921 aacggctaaa caatcaaatg tgaagctact agtagagtta aagccacatg gtaaagaacc
     4981 agcagattat acacaacgtg ttattgatat tttgaaaaag catggtgttg aacatcaata
     5041 tcgtgtgatg tctttggatt atgatgtgat gactaagttg aaaaaagaag cgccatatct
     5101 caagtgtggt tatatcattc cgttgcagtt tggtcatttt aaagaaacat cattagattt
     5161 ctttgtcatc gaagattttt cttattcgcc aagacttgtt aatcaagcgc acttggaaaa
     5221 taaagaagtc tatacttgga ctattaacgg cgaagaagat ttaacgaaat acttacaaac
     5281 caatgttgat ggtattatca cagatgaccc agcattagct gatcagatta aagaagaaaa
     5341 gaaagacgaa acatacttcg atcgttctat aagaattttg tttgaataat ataaacaaag
     5401 acctctaaag ttatcaagat gataccttca gaggtctttt taatgttgcc atctatggga
     5461 taggcaatcg tttcattcgt ttatattcat atgacaagta tttgtatggc aatttggcgt
     5521 cacaaacact tacatgattt attggtgaat tattaattgt tttgtgaatg caaagggtta
     5581 gaaattgaat tgtaaatact ttctaatctt tgtttcgctt tagtcatttg atccaaattt
     5641 ttagtgcgta tagcggattt tgcaatatag tgcgcagcta aaatatcgcg tttttgaaac
     5701 gcatctaaat ttaggtacga taatttattt aagtcagtgt ttgctattaa ttcatgtaat
     5761 tgatctacaa gcgcttgatg ttgatacgta tgtgatgtag tttcagattt gcttgctaat
     5821 ttaataccag tcgtatcaag gagcgccgct ttaataccag caactaaata tgttttgatt
     5881 ttcatttgtg ttgtcatgct ttgttactcc tttgatgtac attaatcaaa aaaattatac
     5941 actattgtat attgcaaagc taattaacta taacaaaaag atagttaatg ctttgtttat
     6001 tctagttaat atatagttaa tgtcttttaa tattttgttt ctttaatgta gattgggcaa
     6061 ttacattttg gaggaattaa aaaattatga aaaagcaaat aatttcgcta ggcgcattag
     6121 cagttgcatc tagcttattt acatgggata acaaagcaga tgcgatagta acaaaggatt
     6181 atagtgggaa atcacaagtt aatgctggga gtaaaaatgg gacattaata gatagcagat
     6241 atttaaattc agctctat
//
LOCUS       DAKTIP010000153         6255 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.155, whole genome shotgun sequence.
ACCESSION   DAKTIP010000153 DAKTIP010000000
VERSION     DAKTIP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.155"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(15..90)
                     /locus_tag="O0305_002131"
     tRNA            complement(15..90)
                     /locus_tag="O0305_002131"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ala,seq:tgc)
     gene            complement(114..187)
                     /locus_tag="O0305_002132"
     tRNA            complement(114..187)
                     /locus_tag="O0305_002132"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Pro,seq:tgg)
     gene            complement(208..284)
                     /locus_tag="O0305_002133"
     tRNA            complement(208..284)
                     /locus_tag="O0305_002133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Arg,seq:acg)
     gene            complement(290..378)
                     /locus_tag="O0305_002134"
     tRNA            complement(290..378)
                     /locus_tag="O0305_002134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Leu,seq:taa)
     gene            complement(386..460)
                     /locus_tag="O0305_002135"
     tRNA            complement(386..460)
                     /locus_tag="O0305_002135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Gly,seq:gcc)
     gene            complement(494..566)
                     /locus_tag="O0305_002136"
     tRNA            complement(494..566)
                     /locus_tag="O0305_002136"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(531..533),aa:Lys,seq:ttt)
     gene            complement(573..648)
                     /locus_tag="O0305_002137"
     tRNA            complement(573..648)
                     /locus_tag="O0305_002137"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(613..615),aa:Thr,seq:tgt)
     gene            complement(665..740)
                     /locus_tag="O0305_002138"
     tRNA            complement(665..740)
                     /locus_tag="O0305_002138"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Val,seq:tac)
     gene            complement(753..867)
                     /gene="rrf"
                     /locus_tag="O0305_002139"
     rRNA            complement(753..867)
                     /gene="rrf"
                     /locus_tag="O0305_002139"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="O0305_002140"
     CDS             complement(1425..2912)
                     /gene="lysS"
                     /locus_tag="O0305_002140"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCY0275260.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2965..3057)
                     /locus_tag="O0305_002141"
     CDS             complement(2965..3057)
                     /locus_tag="O0305_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275261.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3451..3927)
                     /gene="folK"
                     /locus_tag="O0305_002142"
     CDS             complement(3451..3927)
                     /gene="folK"
                     /locus_tag="O0305_002142"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCY0275262.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3924..4289)
                     /gene="folB"
                     /locus_tag="O0305_002143"
     CDS             complement(3924..4289)
                     /gene="folB"
                     /locus_tag="O0305_002143"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCY0275263.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4267..5070)
                     /gene="folP"
                     /locus_tag="O0305_002144"
     CDS             complement(4267..5070)
                     /gene="folP"
                     /locus_tag="O0305_002144"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCY0275264.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            complement(5286..6218)
                     /gene="cysK"
                     /locus_tag="O0305_002145"
     CDS             complement(5286..6218)
                     /gene="cysK"
                     /locus_tag="O0305_002145"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCY0275265.1"
                     /translation="MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
BASE COUNT         1895 a         1357 c          878 g         2125 t
ORIGIN      
        1 gtgtaaaaaa taaatggtgg agactagcgg gatcgaaccg ctgacctcct gcgtgcaaag
       61 caggcgctct cccagctgag ctaagccccc ataaaatgga atttaagaag taatcgggaa
      121 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
      181 cttcccgtta ttcaattaat ttaaaaatgg cgcgcccgat aggagtcgaa cccataacct
      241 cttgatccgt agtcaaacgc tctatccaat tgagctacgg gcgctaaaat ggtgccgagg
      301 accggaatcg aaccggtacg gtgatctctc accgcaggat tttaagtcct gtgcgtctgc
      361 cagttccgcc accccggcaa aataatggag cagaagacgg gattcgaacc cgcgacccca
      421 accttggcaa ggttgtattc taccgctgaa ctacttctgc attattttgc taaatataaa
      481 aattaaatcg taatgagcca tagaggattc gaacctctga ccctctgatt aaaagtcaga
      541 tgctctacca gctgagctaa tggctcaaaa gatggtgccg gccagaggac ttgaaccccc
      601 aacctactga ttacaagtca gttgctctac caattgagct aggccggcta tatctaagaa
      661 taaatggtgg agaatgacgg gttcgaaccg ccgaccctct gcttgtaagg cagatgctct
      721 cccagctgag ctaattctcc gatttgtcat ttgcctggca acgttctact ctggcggaac
      781 gtaagttcga ctaccatcga cgctaaggag cttaacttct gtgttcggca tgggaacagg
      841 tgtgacctcc ttgctatagt caccagacaa aataacttac ttattcatga tacacgctta
      901 attaataagg tgcaatagtt aattttacac tttgatgtaa actttgtcac atcagctttt
      961 agtgtgtttc gtctgttttt ggcgactttt atatattact tcattcaatt gataaagtca
     1021 acacttttaa tcaacttttt tattcttcaa aactgacatt tctcgtgccc tctaattcat
     1081 aacactttta gtattctatc atacacatat gtacaaaacc atacacattc tatacaattt
     1141 taaagtactt ttatagctga ttaagataaa ttatactaat atagcaattt ttgttataag
     1201 atatctcaat gtctatcgtt aacatttctc atccgaacta ctctatatac gtatcgtcat
     1261 tttcatctat cagccaacta ccttgtttaa cagtcaaaaa tagcaccact atcgttaact
     1321 attactatca aaaacattca aacacgaact aattaccata attaagaaag ccctcgcata
     1381 cacatgttgt atacacgaga gctcttacta acaatcaacg tcatttattt ttgtctcata
     1441 taagggaata ataatacgtc tctgattgat ggagagtcag ttaataacat aaccaatctg
     1501 tcaataccga taccaagacc acctgtcgga ggcataccat attctaacgc ttcaatgtaa
     1561 tcttcatcca tttcatgcgc ttcatcatta ccttgcgctt tttcaacaag ttgcgcttca
     1621 aaacgacctt tttgatcaat aggatcattt aattcagtaa atgcatttgc atgctctcta
     1681 cctacaatga acaattcgaa acgatcagta aatctaggat cttcaggatt tttcttcgct
     1741 aaaggtgaaa tttcagtcgg atgaccatag ataaacgttg gctgaataag tgtttcttca
     1801 actttttgct caaagaattc atttaaaata tgaccatatt tcattgtatc tttaatttca
     1861 ataccatgtt ctttagctaa agctttagct tcttcatcac ttttaacttc ataaaaatct
     1921 acaccagtag cttcttttac agcatcaaca atatgcaaac gagtccaagc agattctaaa
     1981 tcgatcgttt ccccattgta ttgtactttt gcagaaccta acacttcatt ggcaatatgt
     2041 ctcaccatag attctgttaa atccataatg tcatgataat ctgcatatgc ttcatataat
     2101 tcaatcattg tgaattcagg gttatgtcta gttgatacac cttcattacg gaatactcta
     2161 ccaatttcat atactttttc aagtccaccg acaattaaac gttttaaatg caactcaata
     2221 gcaatacgca tgtataacgt tgcatctaat gcattatgat gtgttacaaa tggtctagca
     2281 gctgctccac cagcaatttg gtgcatcata ggtgtttcta cttccaagaa acctttatta
     2341 tttaaataat tacgcatttc ttgaatgatt ttactacgat taataaatgt acgagtgcta
     2401 tcttcgttcg taattaaatc taaatatctt tgacgatatc tctgttcaat atcctgtaaa
     2461 ccgtggaatt tatccggtaa tggtcgcaat gatttagtta gtagcgtgaa tttcttcgct
     2521 ttaaccgata attcgccagt atttgttttg aacattacac cttcaacacc aacgatatcg
     2581 cctaaatcag catttttcca taaatcaaat tcgtcatcgc caacttgatc tttacgaacg
     2641 taaatttgaa tttgtccagc taagtcctga acgtgtgcaa atcctgcttt acctttacca
     2701 cgcttagtca ttaatcgtcc agctatagcg acatgactat ccgcttcttt ttctaccaat
     2761 tcttctttag aatactggtc ccactcttct ttcaaatcac tagataaacc tgaacggtca
     2821 aatttagaac caaacgggtc tataccaaga tcatataatt cttgtaattt ttgacgtcga
     2881 accaacattt ggtcattcat ttcttctgac ataactttct ctcctttaac ttgttgtaat
     2941 cctttagtat tccaactggt acactcaaac attttccttt aaagttttat aaaatatcag
     3001 acatttttgc tcaacataca tctctttcga tgtttctaat actttatcat taaccattac
     3061 accttaaagt tatgatgtgg catgttttct tatattcata acatcaattt tatcactttt
     3121 ttaattttac aagagcttgt aaatgtaata aagcaatttc tgttttcgtt tatgaattac
     3181 ttattcattt aaaatacgat tatcattaat acaaccctaa gtaaatgtat aaaaattatc
     3241 ttccacaaac ttcaacaaaa gcctaaataa attacagcaa tttatcaaat attgcttact
     3301 ttgattttat gaaataactt aattctaaca catactaaat catcatatac taattcgaaa
     3361 tcaaatgcat ttagagataa tcaaaatgcg gaaacatctc caataatcaa taatctattc
     3421 ccaataaata tgaatgttct caacaataca ttatttatat ctctttacac tgtcatcgac
     3481 aaaaactaaa tctttcactt tcaatttcga acgtggttct acgacatttg ctgctatatc
     3541 atttaatggg attaaaacaa atgcacgttc attcattctc ggatgtggca ccgacagttt
     3601 tggtaaatct atcatttctt ctccatacaa caaaatatcc acatctaaag ttctaggacc
     3661 ccatcgttcc tttctaatac ggtgtaaaca ttcttctgtc ttcaaacaac attccaacag
     3721 ttgtaatact gtgagtgttg tttgaatttc aacacacaaa tttaaaaagt taggttgctc
     3781 agtataccca actggtgctg tttcataaat cggagaaata ttagatacgt taataccatc
     3841 atattcattc aaaatcttta tagcatcgtt taactggctt tctctatcac caatattact
     3901 acctaatcct aagtatgctt gaatcattta ttctccctca ctatttcgat acctactcca
     3961 tcataatgac ccggaatcgg tgggttttct ttagtgattc tcactttcgt ttccattaca
     4021 cgattatatt gtgaatttat acgatttgca atacgttcag ctagatgctc aagtaaatta
     4081 acggccttac cttccataat tgatttaacc tcttcgaaca cttcaccata atgaactgta
     4141 tcaataacat tatcagtacg cccagcttca gctaagtcta ctttcaaagt tacatccact
     4201 ttgaaaattt gccctatttc attttcagct gataaagcac catgatatcc ataaaagcgc
     4261 atacctttaa gaaagattgt gtcttgcatt ttcattctcc tttaaaaaat ctataccttt
     4321 agctaattta gcattcaact cgacattatg aacgcgtact gctctaacgc ctttcataat
     4381 accatatgca gtcgtagctg cagttacttc atctctttca accggtgttg tatcataacc
     4441 catcatctct ttagtgaaac gtttccggct tgtcgctaat aaaactggat attctgttgc
     4501 aacaagttca tccagtcttg ccataacttc ggcttcttca tttctagttt tagcgaaacc
     4561 tatacctgga tctagccaaa ttttatttga aggtatacca gctattttag cttgatgtgc
     4621 ttgtgctaac aaagatgtta acatttcttc gacaaccggt tcatcacgat taccatttcc
     4681 attatgcatt aaaataattt ccgcgtcata tttagctaca atttggaaca tacgatgatc
     4741 atacagaccc gcccattgat cattaatcat atcaacgcct aattttaaac atgcttcagc
     4801 aacctcactt cgaaatgtat cgactgaaat ttttacatca aaaccgacaa tagcttcaac
     4861 aacaggtaat actctgttca tctcttcttc taatgaaacc atttcatgac cgggtctcgt
     4921 tgaaacacct ccaacatcta taatgtcagc accttcatct atcatggctt tcactctatt
     4981 tatagctgat tcaacattat taaattttcc accatctgag aatgaatcag gtgtgacgtt
     5041 taatatgccc ataatttttg ttttagtcat gttaaccacc ctttaattat gtattgttta
     5101 actctaataa aatttactcc tgctatttac tagttattag tatgtacgtt tattatctta
     5161 tcattcactg gatattttta tcagaaaaat gtttaattta acgctcatat ttttaaaaat
     5221 atacatggta gaaaaaaaga gcaagttatc tcaaaaagat aactcactct tttaaatgac
     5281 attaattagt catcgaatga atataaaggt gttgataagt agcgttcacc attactcggc
     5341 aatactgtta ctactgtttt accttttcct aattcttttg ctttttgaat ggcagcataa
     5401 atcgcagcac ctgatgaaat acctgctaaa ataccttcct ctttagcaac tcgacgagac
     5461 atttccatcg ctgtatcatt tcctacttta ataatactgt catagatttc tgtattcaaa
     5521 gtgcctggaa taaatccagc acctaaacct tgtaatttat gtggacctgg ctcaccaccg
     5581 ctcaatactg gagaagcctc aggctctata gcaacaattt cgatgttagg atattctttt
     5641 ttcagaactt tacctacacc agataacgta ccaccagtac caacaccagc taggaacgca
     5701 tcgatagttt tcccttcaaa ttgttgtaat aactcaggac ctgtagttaa ctcatgaact
     5761 tcagggttcg cagggttttc aaattgttgt ggctcgaagt aaccatgttc ttctttcaat
     5821 tctttagctt ttttaattgc acctttcatc gcttctgatc caggcgttaa aactaattcc
     5881 gcaccgtatg cttttaataa attacgacgc tcttggctca ttgtttcggg catagtaaat
     5941 actgctttat atcctttagc agcacataca aatgctaaac cgatacctgt attaccactt
     6001 gttggttcta caattgtatc gccaggttta attttgcctt ctcgctctgc tttttcaatc
     6061 attgctaaag caattctatc ctttacagaa ccacctggat tttgatattc caattttaca
     6121 taaacatctg ctgcattgtc atctactaca tttctcaatt tgactaccgg tgtaccgcca
     6181 ataatttgag taatattatc tactggtttt tgtgccatta agaacactcc tttttagtta
     6241 gttgttgaaa tctta
//
LOCUS       DAKTIP010000154         6120 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAKTIP010000154 DAKTIP010000000
VERSION     DAKTIP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.156"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(572..850)
                     /gene="qoxD"
                     /locus_tag="O0305_002146"
     CDS             complement(572..850)
                     /gene="qoxD"
                     /locus_tag="O0305_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCY0275266.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(859..1464)
                     /gene="qoxC"
                     /locus_tag="O0305_002147"
     CDS             complement(859..1464)
                     /gene="qoxC"
                     /locus_tag="O0305_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCY0275267.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(1454..3442)
                     /gene="qoxB"
                     /locus_tag="O0305_002148"
     CDS             complement(1454..3442)
                     /gene="qoxB"
                     /locus_tag="O0305_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCY0275268.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(3442..4542)
                     /gene="qoxA"
                     /locus_tag="O0305_002149"
     CDS             complement(3442..4542)
                     /gene="qoxA"
                     /locus_tag="O0305_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCY0275269.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            4844..4966
                     /locus_tag="O0305_002150"
     CDS             4844..4966
                     /locus_tag="O0305_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275270.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(5114..5431)
                     /locus_tag="O0305_002151"
     CDS             complement(5114..5431)
                     /locus_tag="O0305_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCY0275271.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2104 a          992 c         1055 g         1969 t
ORIGIN      
        1 aaccttacaa tacgaagggt gttattgttc aataattaat atagaagata taacatgtat
       61 atggcattaa ggcatcgacc ttatctgacc agtatacgag ttatatcttc ttttttatag
      121 tggtaaaaag tttaaagtat aaggttgaag aaggatgagt ttaaaaatat gtgttaactg
      181 ataaaagggg aaatcatttg gtgagttggc atcagactaa aatgaatgaa gacgaatacg
      241 ttggtccatg cgtggtgtga atgttttcta aatctaactg gaaatgataa tttaataatg
      301 tacacttttg attgtttaaa catgtacaat ttttaaaaac ctagggataa ttttattgac
      361 gtgaacaaca ttgagattat ctttagacat aaatatggat tttgaagtta ctatatttat
      421 tagttgtggt tgttaatgta ttaattgaat tgattataat gattgtctta taagatatag
      481 aaggggactt aaccatttaa aaattgaagg aaaataaaaa ccagctaaca aaagtgttag
      541 ctggttaaac tttacattat taaaatgttg tttataagtg tgaagagtga ccgccttgca
      601 taacccaata tgttccgaca acgaaacaaa gtgtaattac aagagcaaag ataactttga
      661 atgtttgtaa acgtccatct ttaccttcag ttaaatgcat gaacattaat aattgaagtc
      721 ctgcttggac gaatgcaaag ccaaagataa ttgtcaactt cgcgtggaat gttaatgacg
      781 tgtatagtgt tacgtaaact gctaaaagcg ttaatacgat agatgcgata aatcctacag
      841 tatgtttcat tattgtactc atccgctata caccatccct atcatatata cggcagtaaa
      901 gatgaaaacc caaacaacat ctaagaagtg ccagtataaa cttactataa ataattttgg
      961 cgcattatat ttgtctaatc cgcgtcgttg gatttggatt aataaacaaa tggcccaaac
     1021 gatacctagc gatacgtgac aaccatgcgt tcctaatagg ataaagaaac tagaccagta
     1081 agaaccaatt gttgggttaa cgccttctga tgcatagtgt gcgaattcat aaatttcgaa
     1141 tccaacaaag actaaaccta aaagtaacgt aatgatcatc caaaacatca ttaacttttg
     1201 tttttcttgg cgcatgtagt aaatagcaat accacatgtg taagaactga ataataatgc
     1261 aaacgtcatt attaaaacaa gaggcaattc aaataactca gtagtcattt tacctgcata
     1321 atcgccacca tgttgcaaag ttaatagtgt cgcaaatagg gtaccgaata acgcaaattc
     1381 ggctgtaatg aaaatccaaa agccaagctt atttaattcg ccttcatgtg tgcgtgaatc
     1441 aatagtgttt gtatcatgac tcatgactta cagcctccct ttctttaata cgcgcttctc
     1501 ttaatctagc ttcagtttct gcaacttcag cagcagggat atgatatccg tgatcgattt
     1561 ggaaactgcg ataaatcata gtaccaaaaa taccgaataa acaaattaat gctggaatta
     1621 cagtttcgaa aattaagaag aaaccgccga tagtcataaa gataccaatc cagaatccaa
     1681 caggagtatt gtttggcata tgaatgtctt tgtaattatg gttgtctaag taatgacgac
     1741 catgttcttt catatcaaca aatgtgtcgt agtcattcca atctggtgta atggcaaagt
     1801 tgtatttagg tggaattgct gatgcagtag tccactctag agtacgacca aggccatccc
     1861 agttatctcc agttgcttca cgtggagatt tgaagtgact gtatacgata ctaacaacaa
     1921 ggaataagaa tccgattgcc attaataatg caccgatagt tgaaattaag tttaataaga
     1981 accaaccatc tgatggcata taagtgtata aacgacgtgg cataccatct aatccaagaa
     2041 tgaattgtgg taagaaacaa acgttaaatc cgatcatgaa gaaccagaag caccatttgt
     2101 ttaatgtttc gtttaatttg taacccatca tctttggata ccagaagatt aaaccagcta
     2161 agcaggcaaa tacaacacca gtaaccaatg tatagtggaa gtgagctact aagaagtacg
     2221 tattgtgata ttgatagtca gctgatgcca ttgctaacat tacacccgta acaccaccta
     2281 aaaggaagtt agggataaat gctaatgaga atagcattgg tgactcaaat gtaatacgtc
     2341 ctttatataa tgttaataac cagttaaaca atttcacacc agttggaata ccaatcagca
     2401 ttgttgaaat tgagaagaat gagttgatta acgcaccatt acccattgtg aagaaatggt
     2461 gaacccaaac taagaaacta aggaacgcga tgccggcagt tgcccatacc atactttgat
     2521 gtccgaataa acgcttacga gcgaatgtcg ggataatttc tgagtaaata ccaaatgctg
     2581 gaaggataac gatataaact tcagggtgcc cccataccca gaagaagtta gcccaaagca
     2641 ttggcatacc gccatgtgca actgtgaaga atgctgtgtc aaatattcta tcagttgtca
     2701 ttaatgctaa cgctactgtt aaaggaggga aagcaagaat aacaattaat gtagtaataa
     2761 atgttgttac tgtaaacatt ggcatttgca taaacttcat agttggtgtt ttacatctta
     2821 aaattgttac aaagaagttg atacctgtag ctaaggtacc aagccctgaa atttgtatag
     2881 ctattaagta atagttaaca cccggaccag gactgaattc acctgctagt ggcgcatagt
     2941 ttgtccaacc agctgctggt gaaccaccaa taataaatga caggttgaat aaaatcatac
     3001 ctgcaaagaa tagccagaaa cttacgttgt ttaatactgg gaatgcaaca tcacgtgctc
     3061 caatttgtaa tggaacaacg atattccata aaccaaagat aaatggcatt gccatgaaga
     3121 taatcatgat tacaccatgt gtactaaaaa tttcgttata gtggttagat tctaaaaatt
     3181 tgttatcagg tactgttaat tgcgcacgaa taagtaacgc atcaatacca ccacggacga
     3241 acattaatac ggcacagatt aaatacataa taccgatttt cttatggtct acagatgtga
     3301 accattcttt gtaaagatat ttccataatt taaagtaagt aattactgcg attaaaccaa
     3361 taactaagaa tggggcacca atttgtgcca ttgtaatcat ccagttacct ttaactagta
     3421 attgatccca tggaaaattc attaatgtcc acctccatga tcatcattgt cttgatcttg
     3481 cgcatctttt gaaattttct tcatttcttt cgcatttttc gattcatctt tcttgaactc
     3541 attgttatat ggttcgtcat ttccaagaat catcaacttc ataccatgtc gtttatagtt
     3601 cgcatttgta atttgagctt tacgagcagg tattaatggt ttgtctgata catctttaaa
     3661 catattttct tcactagtga agtttggatc ttttaattcg aaattgaaac gtttatatgc
     3721 atagaagatg tattctggat cggctgctgg atcaacaaac gccatatgtg taccattaaa
     3781 ttctaaagct ttattaggtg tgcttggtaa taattgttta tcaaatgtat cttgatctaa
     3841 cgttttctta cctttaactt ctttcaccca tttgtcgtag tctttttgac taacggcatt
     3901 tactttaaat gtttgacgtg agaatccttc accattgaag ttagagttac gacctctgaa
     3961 cgtaccagtt tgagatgctt ctaacgtcca attcattgtc atgccagtca tggcatattt
     4021 ttgaccacct aattgtggaa tccagaaact tgtcattgta tccatagctt gaagcttaaa
     4081 tacaacagga cgatctttag ggattgttaa tgtattaaca gtctctatat gttcatctgg
     4141 ataagcaaag aaccatttgt atcctgcact tactgcatat acaaccattg gatctttctc
     4201 actcttcggt ggtttttcgt aatcgtataa agttttaact gtaggaatag ctaaagcagc
     4261 aacgattatg ataggtatta caaaccatat tgtttcaatg atggcattat ggtgcatctt
     4321 accagattcg gcattcttat tataactata cttgtaaata aaaatggcga acatgccaag
     4381 tacaacgaaa caaataacaa gcatgaagac gattgaataa agaatcaaga acttctgact
     4441 acttgctact ggcccttttg cgttgaaaat ttctatattt gaacaaccac taagtaaaat
     4501 tagtgtgcca aataatagaa gcaaagactt aaattttgac acttttttga cctcctaata
     4561 ctacaaatgt agggcttaac attaatttta agttattaca caatatttac aagggcttat
     4621 gggaaaaaaa ttaataaaat tgtatcaaaa atgttgataa atcaaggtgt gacgtgggtt
     4681 cacacatttg ttaaaattat gtgtacattt tgtgactaat agcgtttttg aaaaaattgc
     4741 ttcatattta aaaggaaagt tgagaatagc tttcaattaa aggttaattg aatgaaaata
     4801 aaaaaagttt gaaattgtct aataaattga aaatgatggc tagttgattg aaaggaagcg
     4861 caaacatttt aataaggaat ttgccaaaag taatttatcg ttaaacaatg aaaagtggaa
     4921 atttgtttgt tcaaaatcaa aacatttttt taaaaagagc agttaagttt taataatttg
     4981 ataggtagtc ctttggtata cagaaaaaat acaatatttg ggttaaagtt gcagatgaag
     5041 ttagaataac aaataaaatg gtagcaaaac atgttcgttt gctaccattt tatttgcagt
     5101 atagtgatga gggctattta acctcaatat acctagtgga aattttaatt gcaccatctg
     5161 aatcagtgac atgatattta atttgatatt taccagattt agatgtatca atttggccat
     5221 cgactttaat tttatcggtt aaatctccat cttctttatc aaatgcactt atgccgttta
     5281 gaaggttata atcttgacct ttcttaataa cgatatcatt agcgccttta atttgtggtg
     5341 tagtattcgt cgttgcatct gcatttaaat ttggtgttac tagtgtagca gaaacaccga
     5401 gggctgataa tgactgtaat agtttattca taatgtaacc tccgaatgat aagtcatttc
     5461 tgagttagct ttactatacc ttaatttata ttatttacac atcatactat agtcctaatc
     5521 ttaccctata aagaatgaaa tttaaatata aatttataaa taatgaggaa ataattatta
     5581 ttttgaaaat ctgccacaat tgtaaaatta ataatttagg gataatttga aatcaaatat
     5641 aactttattc gacagtttga acttaaattt ttgtgtatca aaacaataag aaaagggagg
     5701 cgaaaatgat aaagaaccac taatgattaa ctatgtagta gtactttatc atagtcacag
     5761 cttctgtgtt ggggcccatc cccaacttgc acattattgt aagctgactt ttcgtcagct
     5821 tctttgttgg ggccctgact agaattgaaa acttgttaca agcttatttt cattcaatta
     5881 actactgtct atataaaatt gttgagccaa gtacaaagtg aaatgctccc ttcaaagcct
     5941 tcatttattc aatgtctact ttgaagggag catttgatcg attaatgtcc cagatttttc
     6001 ttgttattca ttgcaattgc aaaataatat ttaatgtggg ttaaaagact ttattaaaac
     6061 tcagttatta gggagtggga cagaaatgat attttcgtaa aatttatttc gtcgtcccac
//
LOCUS       DAKTIP010000155         6071 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAKTIP010000155 DAKTIP010000000
VERSION     DAKTIP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.157"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            51..305
                     /locus_tag="O0305_002152"
     CDS             51..305
                     /locus_tag="O0305_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCY0275272.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(308..697)
                     /locus_tag="O0305_002153"
     CDS             complement(308..697)
                     /locus_tag="O0305_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275273.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            767..1309
                     /gene="rsmD"
                     /locus_tag="O0305_002154"
     CDS             767..1309
                     /gene="rsmD"
                     /locus_tag="O0305_002154"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCY0275274.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            1311..1793
                     /gene="coaD"
                     /locus_tag="O0305_002155"
     CDS             1311..1793
                     /gene="coaD"
                     /locus_tag="O0305_002155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCY0275275.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(1855..2994)
                     /locus_tag="O0305_002156"
     CDS             complement(1855..2994)
                     /locus_tag="O0305_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCY0275276.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTSNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            3121..3678
                     /locus_tag="O0305_002157"
     CDS             3121..3678
                     /locus_tag="O0305_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCY0275277.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            3758..3931
                     /gene="rpmF"
                     /locus_tag="O0305_002158"
     CDS             3758..3931
                     /gene="rpmF"
                     /locus_tag="O0305_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCY0275278.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(4085..6022)
                     /gene="isdB"
                     /locus_tag="O0305_002159"
     CDS             complement(4085..6022)
                     /gene="isdB"
                     /locus_tag="O0305_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCY0275279.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEE
                     TGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEV
                     KAPKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDG
                     TQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVK
                     DYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAE
                     KLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQN
                     VQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYW
                     KDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVD
                     KEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPS
                     VEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVV
                     QTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLP
                     LMALLALSSIVAFVLPRKRKN"
BASE COUNT         1886 a          847 c         1062 g         2276 t
ORIGIN      
        1 aataaagaca tgatatgatt aatgctgaaa ataaatggag tgaggtttaa atgaatttaa
       61 tcccaagaac tagtattgta gtttatttaa aacatatgaa acatgaacga caaatccgaa
      121 aatatggaca tatcgttcat tcaaatagag atcgtaaatt tgtaattatg tatgtgaatg
      181 agcaagatgt tgatcaaatt gtacataaac taatgcaact taaatacgtt agacacattg
      241 atggctcacc atataaatac ttaaagaaaa cttacgaaaa agagaaacac gaaatatata
      301 attaatatta ttgaagttct ggtatccatt tttggagcct taacacacta attaaataat
      361 tataatataa cgataaatca tgaaatgctt gagggggctg tacatcgata cctaaaattg
      421 gtatgtcgaa tttttcagca caatcattta tcatttgaaa ctttttattt gaatctggat
      481 atggatattt aatagcgttt atcatgatat aaaatgcttg gaaatgattg ccagttgtag
      541 ggttcataat gattgctaca gatgactttt tattttcagc ttgtggtgaa tgaaaataga
      601 taattttatc aatacgttga tggttatatt caatcatcgt catgaattcg aacaaatctg
      661 ttagaccttc accaagtgta ataaatgttt gtttcattgg tttatcattc tcctttttct
      721 caataaatta tattaaataa ctaaatgaat tggaagtgtg aacaacatgc gcgtcattgc
      781 aggtaaacat aaaagtaaag ctttagaaag tatggaaggc cgtaatacga gaccaactat
      841 ggataaagtt aaagaaggta tctttaatag tttatatgat gtgtcaggta taggtttaga
      901 tttatttgca ggaagcgggg cgcttggaat agaagcactc tctcgaggta tggataaggt
      961 aatctttgtt gatcaaaatt ttaaagctgt aaaagttatt aaatcaaatc ttgcgaattt
     1021 ggatttagag gcacaatctg aagtttataa aaataatgca gatagagctt taaaagcatt
     1081 gtcaaaacgt gatattcaat ttgatgtcat tttcttagat ccaccttata ataaaggtct
     1141 cattgataaa gctttaaaac taatttcaga gtttaattta ttgaaagaaa atggtatcat
     1201 cgtttgtgaa tttagcaatc atgaagaaat agattatcaa ccgtttaata tgattaaacg
     1261 ttaccattat gggttgacag acacattgtt attagaaaag ggagaatagc atggaacata
     1321 caatagcggt cattccgggt agttttgacc ccattactta tggtcattta gacattattg
     1381 agagaagtac agatagattt gatgaaattc atgtctgtgt tcttaaaaat agtaaaaaag
     1441 aaggtacgtt tagtttagaa gagcgtatgg atttaattga acaatctgtt aaacatttac
     1501 ctaatgtcaa ggttcatcaa tttagtggtt tactagtcga ttattgtgaa caagtaggag
     1561 ctaaaacaat catacgtggt ttaagagcag tcagtgattt tgaatatgaa ttacgcttaa
     1621 cttcaatgaa taaaaagttg aacaatgaaa ttgaaacgtt atatatgatg tctagtacta
     1681 attattcatt tataagttca agtattgtta aagaagttgc agcttatcga gcagatattt
     1741 ctgaattcgt tccaccttat gttgaaaagg cattgaagaa gaaatttaag taataaaaat
     1801 aacagtattt taggtttatc atggtttaca atcctaaaat actgttttca tttgttaacg
     1861 atattgctgt atgacaggcg tgttgaaatc tgtttgttgt tgcccgctta ttgcattgta
     1921 tatgtgtgtt gctttgattt catttgtgaa gtaatgtgca ttgcttttgt taatattggt
     1981 tatatattgt ctttctggga acgctgtttt taaatgcttt aaatattgtc tgccacggtc
     2041 gttcatcgct aatactttaa ctgcgtgaat gttactcgta acatctgtag gtttaatgtt
     2101 taataataca ttcattaaca gtctttggat atgcgtatat gtataacgct ttgtttttag
     2161 taattttaca aaatgatgaa aatcagttgc ttcataaatg ttagatttca aacgattttc
     2221 aaaaccttca gtaacagtat aaatattttt taatgaatct gtagtcatag ctatgatttg
     2281 atatttcaaa tatggaaata tttgatttaa tgttatatga ggtgttacgt acaagtgttg
     2341 aatatcttta ggtaccacat gatgccaatg atcatcttga ctaatgattg atgttctaat
     2401 agatgtacca cttgcaaact gatggtgttg aattaatgaa tcatgatgtt gagcattttc
     2461 tcgtttgata gaaattgcat tgatgttttt agcattttta gcaattgctt tcaggtaact
     2521 aataccaagt atgttgttag gacttgctag tgcttcatga tgctctaata attcgctaat
     2581 gatacgaggg tagcttttac cttcttttac tttttgtgaa aaggattcag attgttcaat
     2641 ttcattaatg ctgtgtgcta attgctttaa tgttttgata tcattatttt cactaccaaa
     2701 tgcaattgta tcgacactca tataatctgc gactttaact gctagttcgg caaaatgatc
     2761 gccagatgat aaactggcag ttgctggtag ttcgataact aaatcagctg ttgataatgc
     2821 catttttgca cgagtaaact tattatagat tgctggttcg ccacgcatga caaagttacc
     2881 actcattatt gcaatagtaa cgtcagcatt tgtaagtttt ttagattgat taatatgata
     2941 ttgatgccca ttatgaaagg gattatattc tgtgattaag ccaacgcttt tcatttgcaa
     3001 ttcgtccttt cggtattcat tattaatatt gtaacagaac atgatatgtt aagaaaaaat
     3061 cttgacaact tgttcttaga aagttaaaat aaattttgtg cttgttagag gtgaagccat
     3121 atgaaatggt caattacgca attaaggaaa tatcaaggta agccatttga atttgatcaa
     3181 acggtgagtt ttgacaattt aaaagaatca ttagatttaa ttgatttatc tccaattaca
     3241 atccaaggtc agttaaccat taagtcaaca gaagtcgttg cggatattca cattactgga
     3301 acgtatacaa tgccttgtgc acgtactctt gtaccagtaa aagtcccact agatgtaact
     3361 actacagaag tatttgattt agaagggtac aatcagtata acgatgatca agatgatgta
     3421 gatgaacact atcacattat taaagatggt atggttaatc ttcaggatat tgtcgaggat
     3481 atagttatta ttgagaaacc aatgagagct tattcagagc aaagtgacca aatgttgaca
     3541 gtaggtaatg gttgggaagt aatcgatgag gatcaattag atgagcttgc taaacagcaa
     3601 gaacaagatg attcagaatc acgacaagtt gatccaaggc ttcaaaaatt acaacaatta
     3661 tatgataaag agcaataagt agtttaatta atgatataat gttgttaatt aaactaacat
     3721 ttagtatttt tcgtttataa tctaaggagg atatatcatg gcagtaccaa aaagaagaac
     3781 ttctaaaact agaaaaaaca aacgtcgtac gcatttcaaa atttcagtac caggtatgac
     3841 tgaatgccca aactgtggcg aatacaaatt atcacaccgt gtatgtaaaa actgtggttc
     3901 ttacaatggc gaagaagtag cagctaaata attttagtta ctcaatataa aaagtcccgc
     3961 ttaaaatgat tgttttaagt gggacttttt atattgcgaa aaataattgg cgaacgaggt
     4021 aactggatac ctcatccgcc aattaaaatt tgttaattta ataattaaat ataaagacga
     4081 tttattagtt tttacgtttt ctaggtaata cgaatgcaac gatgctactt aaagctaata
     4141 atgccattaa tggtaatgtc atatctttat ttgattcttc accagtttgt ggtaatgatt
     4201 ttgctttatt ttcttgtgta tttttattgt tttggctttg agtgtgtcca tcatttgtgt
     4261 ttttaatgtt tgctttttgt aatggagcac tatcttttgc ttcgctagaa cctgctgaag
     4321 tttgaacaac atcttttgtt gtttttgatg aagcagttgt tggttttgca acattttgag
     4381 tcgtagatac taccttagtt ggagttgtac tacttgattc tacttcacct ttagttggtt
     4441 ttgtagcagg cgttttgtct ttacctgact cactagatgc gtcattttct ttttcaacac
     4501 ttggtaattg tttattgtca tctttttggc tgtcttgttt ttgtgattct ttttcaacag
     4561 gtgatggtgt tggtttgcta ggcgtagctg gagtagcttc cttcttagct gagttatctt
     4621 gttgttcttt tttgttagat ttatcggtat tggcttttgt aaatgcttct ttatcaacga
     4681 ttctgacatg gtattgtcca tcataatcaa tcgtttttac gtgaacttta acgatagcat
     4741 catatagagt tttaccttca acatatggga aaataattgt tctagtatta tttttagcat
     4801 ctttgcttat agttctaaca cgttgacctt caaccatgaa atctttccag taatcgtcat
     4861 tagtagtttc catgaccata tattttttgc cgttaagcat acctgtttta atagggtgtt
     4921 taacaaaagt atccatcata gattcgttat tctcaacact ttcataaaca acatattttg
     4981 tatcttgtaa atcagtcatt ttttcatttg ttggttgtac attttggaat tcagtaatag
     5041 ctgatttcac ttgctcatct aaagctttct ttgtatcctc taatttcttc ttgtactcag
     5101 cctttaattt ttcaggaagt ttatcttgaa ttttatttaa ttcataaact tgtctttcta
     5161 gtgttttcgc ttttttatat ggcgctaata atttttcagc tttataatct tcttcagttt
     5221 tgaatttatc tgcactgtta taaattggtt gtgcgaattc cattaatgtg taatcgtatt
     5281 tttcttcttt gttattgaag tgtgttgaac taacaatttt aacagctttt gttccgtttg
     5341 atacagagaa gcgaatgtaa gcataatctt taacagtatc gtatgatact aatttaattg
     5401 gcaacttttt gtcaccttca taaacttcaa attttctcca aaattgacct gattgtaatc
     5461 ctaattcaat ttctggtttt gaatcagtga aaataactct agcaggttta acagaacttg
     5521 cataatgata aaactgttga gttccatctt tctttttcat ttcaaaatca attggacgag
     5581 agtttggtgc gctatgatct ttgtctttta ttgcagggtt tttaatcgct tctctaagtt
     5641 cctgattcaa aataggatat gtattgttag tggcttttgc tgctggttta acttcttttg
     5701 tttccttagg ggctttaact tctttaactt ctttagcttc ttttgtttca gaagtagggg
     5761 cctcaacttc tttattagat actgagacag cattagctac tggtttagtt tctggagctt
     5821 tttcagatgt tgttgttgga cttgcaactg cttcagtttt tggttgtgct tctgtatttg
     5881 taccacctgt ttcttcagct gctgcttgtg cttcgccatt tgacattaat aataaaagtg
     5941 tactaattgc tacagatgca acgcctagtg atgactttct aattgaataa aatgatttaa
     6001 attctttttg ctgtttgttc atgttgtaga aacaactcct aattgtatat tatcaactga
     6061 taatcattat c
//
LOCUS       DAKTIP010000156         5968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAKTIP010000156 DAKTIP010000000
VERSION     DAKTIP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.158"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..303
                     /gene="deoC"
                     /locus_tag="O0305_002160"
     CDS             <1..303
                     /gene="deoC"
                     /locus_tag="O0305_002160"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0275280.1"
                     /translation="VKVIIETVLLDHDEIVKASELTKTAGADFVKTSTGFAGGGATAE
                     DVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            582..1883
                     /locus_tag="O0305_002161"
     CDS             582..1883
                     /locus_tag="O0305_002161"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCY0275281.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIGGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1897..2247
                     /locus_tag="O0305_002162"
     CDS             1897..2247
                     /locus_tag="O0305_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275282.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2399..2869)
                     /locus_tag="O0305_002163"
     CDS             complement(2399..2869)
                     /locus_tag="O0305_002163"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCY0275283.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            3180..4364
                     /locus_tag="O0305_002164"
     CDS             3180..4364
                     /locus_tag="O0305_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCY0275284.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGR
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4364..5557
                     /locus_tag="O0305_002165"
     CDS             4364..5557
                     /locus_tag="O0305_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCY0275285.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPLVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
BASE COUNT         2064 a          812 c         1223 g         1869 t
ORIGIN      
        1 gtaaaagtga ttattgagac ggtattgttg gaccatgacg aaattgtaaa agcgagtgaa
       61 ttaacaaaaa cggctggtgc ggacttcgtt aaaacttcaa caggttttgc aggtggcggt
      121 gcgactgcag aagacgttaa attaatgaaa gatacagtag gtgctgatgt agaagtaaaa
      181 gcatcaggtg gcgtacgtaa tttagaagat ttcaataaaa tggttgaagc aggtgcgaca
      241 cgtattggtg cgagcgcagg cgttcaaatt atgcaaggtt tagaagcaga ttcagattac
      301 taattttata aaaatatgta agcatgggac atcgtaacca tatataagta tgtcgatatc
      361 agctttaaga catcataaat gccatgatga ttctaagata atttaatagg gactttaacg
      421 ttataaccaa tctatttaag aataataaca tcgcattgaa attgtgatat taaaaatgaa
      481 atcgatagct tgctataata attttcgctt gtgcttttaa aaatggacta gagaaagcgc
      541 agatgctttg tcctaagctt tttaagaaaa ggatgataaa aatgagaatg atagacatta
      601 ttgagaaaaa gcgtgacggt catacactaa caacggaaga aattaatttc tttattggcg
      661 gctatgttaa aggggatatt cctgattacc aagcatcaag tttagcaatg gcgatttatt
      721 tccaagatat gaatgatgat gagcgtgcag cattaacgat ggctatggtt aattctggtg
      781 atatgataga tttgagtgat attaaaggtg tcaaagtaga taagcactca acaggtggtg
      841 taggagatac aactacattg gttttagcac cattagtagc agctgtagat gttcctgttg
      901 caaaaatgag tgggcgtgga ttaggtcata caggtggtac gattgataaa ttagaagcaa
      961 ttgatggttt tcatgttgaa atagatgaag caacatttgt gaaattggtt aatgaaaata
     1021 aagtggcagt tgtaggacaa tcaggaaatt taactcctgc agacaaaaaa ttatatgcct
     1081 taagggatgt tactggtact gtcaattcaa taccattaat tgcctcttca attatgagta
     1141 aaaagattgc tgctggtgca gatgcaattg tattagatgt aaaaactggt agcggtgcat
     1201 ttatgaaaac attagaagat gctgaagcat tagcgcacgc aatggtacgt attggtaata
     1261 atgtgggacg taatacgatg gcgattattt ctgatatgaa tcagccactt ggacgtgcga
     1321 ttggcaatgc gcttgagtta caagaagcaa ttgatacgtt gaaaggacaa ggtccgaaag
     1381 atttaacaga acttgtatta acattaggtt ctcaaatggt tgtacttgca aacaaagctg
     1441 aaacgcttga agaagcgaga gcgttgctaa ttgaggcaat taattcaggt gcagcattag
     1501 aaaaattcaa aacatttatt aaaaaccaag gtggcgacga aactgttatt gaccatccag
     1561 agcgtttgcc acaagctcaa tatcaaattg aatataaagc taaaaaatca ggttatgtga
     1621 ctgaattagt ctctaacgat ataggtgtcg cttcgatgat gttaggagcg ggacgtttaa
     1681 caaaagagga tgatattgat ttagcggttg gtattgtttt aaataaaaaa attggtgata
     1741 aagtagaaga aggagaatca ttattaacga ttcacagtaa tcgccaagat gtagacgatg
     1801 ttgttaaaaa attagattca agcataacaa ttgctgatca tgttgtatcg cctacattaa
     1861 ttcataaaat cattacagaa taggagttga agctgattga acatactaag atatttatta
     1921 ggaataggtt ttagtgtaat aggtgtattg cattttacac gagaacgaca atttagaaat
     1981 atcataccga aatgtttgcc acttcgaaaa acagcggttc ttgttacagg aatctttgag
     2041 atattatttg gacttgcact tttaattaaa agaccatctc aatgcttgaa aaatacaatc
     2101 aatctattct tgctagctgt attcccagct aatatctata tggcagttaa aaagttacct
     2161 ttaggagata aacaattgcc aacatggcaa ctatatttaa ggttacctat gcagttcgtg
     2221 ttaatgggac taattaagaa attatagtgg ttctcaaata aatcggacaa atgttaaaga
     2281 ttttactttt tcatttgttc gattttttga ctttgaagca taataaaaag cagttggaca
     2341 atggcgattg taaaatcatt gttcgactgc ttttctgatt aacttgattg actaagattt
     2401 attttcctgt accgaaaaca tcatgccatt cgtttcgttt gtctagaaat gcttgagcga
     2461 tagttttagc accttctaat gaatgacttg ctgcccagcc acattgtact tcattacaag
     2521 caggcacttc agtagcattt agcacatcat ttaaagttgc ttcaacaata tttaatacat
     2581 catcataatt atcatgatta ataaatgata catagaaacc agtttggcaa cccataggac
     2641 ttaaatcaac aactttgtca ctatgatttc taatattttc agccattaaa tgttctaatg
     2701 aatgtagtcc gggcatatcc atatgttctt tgtttggttg tttgaaacga atgtcgtatt
     2761 tgtgaatgac atctccgttt aatccttcca tcgttcccgc taatctaata aatggggcaa
     2821 ccaccttagt atgatctaaa ttgaaacttt caacattcat ttttgtcatt ttgaatttcc
     2881 tcctattaac tactcaaaac tataaaacgc ttatgatttg ttgcattata aaacttaagt
     2941 ttgttgtaag ttgtgtttct aacaatactt aaattgtaac aatagggctt atgttttaca
     3001 atttgaaact gttttataaa tttatgaaag gcttttagtt attagaaaat tttgtaaata
     3061 aagaaaaact agatgacatc atgagataaa accgatagaa taagtaataa ttcattttga
     3121 aagaacaaaa tattgtggat gattaaaatg agtgtagggt aaaagggaga tagtaaaaaa
     3181 tgagtgaaaa acaacaaatt ctcgattata tagaaacaaa taaatatagt tatatcgaaa
     3241 tcagtcatag aattcatgaa cgtcctgaac ttggtaatga agaaatattt gcgtctcgaa
     3301 ctttaataga tcgtttgaaa gagcatgatt ttgaaattga aaccgagatt gctgggcatg
     3361 caactgggtt tatagcgaca tatgattcag gacttgacgg gccagctata ggttttttag
     3421 cagaatacga tgctttgcca ggattgggtc atgcttgtgg tcataatatc attggaactg
     3481 caagtgttct tggtgcaatt ggtttgaagc aagtgattga ccaaattggt ggtaaagtag
     3541 tcgttcttgg atgtccagct gaagaaggtg gggaaaatgg tagcgctaaa gcttcttatg
     3601 tcaaggctgg tgtgattgat caaatagaca ttgccttaat gattcatccg ggaaatgaaa
     3661 cttataaaac gattgatact ttggcagtgg atgttttaga tgttaaattt tacggaaaaa
     3721 gtgctcatgc ctctgaaaat gcagatgaag cgttaaatgc attagacgct atgattagtt
     3781 attttaatgg tgtagcacaa ctacgacaac atattaaaaa agatcaacgt gtgcatggtg
     3841 tgattttaga tggcgggaaa gcagctaata ttattccaga ctatacacat gctcgttttt
     3901 atactagagc aatgacgcgt aaagaattgg atatattaac agaaaaagta aatcaaatcg
     3961 cacgtggagc tgcgatacag actggttgtg attatgaatt tggtcgaatt caaaacggtg
     4021 tgaatgaatt cattaaaacg ccgaaattag atgatttatt tgctaaatat gctgaagaag
     4081 ttggtgaagc agttattgat gatgattttg gttatggctc tacggataca gggaacgtaa
     4141 gtcatgttgt gccaacaata catcctcata ttaaaatagg atcacgtaat ttagtaggac
     4201 atacgcatag atttagagaa gcggctgcga gtgtacatgg tgatgaagca ttaattaaag
     4261 gcgctaaaat aatggcgttg atggggttag agttaattac aaatcaagac gtttatcaag
     4321 acattattga agagcatgcg catttgaaag ggaatgggaa gtaatgacaa ataatggtaa
     4381 tgagccgaac ttaacgttaa gtgatttata tgacaaagat gttgtctata cgtcaagacc
     4441 ttcgtatata tcgaatccat ggttgaaacc ggacgaacat caatctaatt ttttaactgg
     4501 tagggagtta ttaatagcaa atcaattacc tgttattgtt cacgaagcta gtgcgacaga
     4561 caaattgcat cagctatttc aagttattgg taaggaagta ccgaactcaa tttatacatt
     4621 taacaatcag caatcttacg aaaacttgat caaacaattg gcacataagg aaaataaaaa
     4681 gatttatttt caatatattc atgatgaaac aatcttgaat cagcaatatt atgcattaga
     4741 taaaacgtta tttgttgctt taaataataa agcaagaata cctgaatgga caaatgggaa
     4801 gtttttacca aaaagaaaag ttgtaaaaat tgagcaattc gaaaacgaga ttaagaattg
     4861 ggaattcccg ttagtaatta agccgggaga tgatcttccg acagcaggtg gctatggcgt
     4921 tatgatttgt tatcatgatg cagatttaca aaaagcgata acgcgtatta aagaggcaac
     4981 tgctgaaacg aattcgctta ttatcgaaca gaagattgaa gaaaaagcga attattgcgt
     5041 tcagtttgca tactcggaat cgctaggcat tcaatatctt ggtgcggcaa cgcaattaac
     5101 ggataagtac ggattttata acggaaatga aaatactact aatgtaccag agcacgttat
     5161 agaagcaggt agacaaatta tggaaaacgg tgtgaatcaa ggattctttg gtgtagctgg
     5221 ttttgaccta ctcgtcgatg aggatgataa cgtttatgcg attgatttaa actttagaca
     5281 aaatggttca acgagcatgt tattacttgc taacgagttg aattcaggat atcaaaagtt
     5341 ttatagttat cattcaaaag gtgataacac acatttcttc aatacgattt tgaaatatgt
     5401 caaagaaggt agtttatacc cgttatctta ttatgatggt gattggtacg gtgaagataa
     5461 agttaaatca aggtttggct gtatttggca tggtgattca aaagaaacag tactggagaa
     5521 tgaacgcgca tttttagctg aacttgaaca ctattagagt tcggaacata aggcgctaca
     5581 atgttgtgtt gccagtagtt gactgaatat gcgtttgtaa caagcttttt tcgattctag
     5641 tcaacagtaa ttaaatttat gatatggcaa tactttgtaa tactaatatt aaatggcgac
     5701 ttttatttca ctatgttata agagttgcca ttttgttgat aaaggtatac taaaggttat
     5761 cgttttgaaa tttttagtaa ctagatatgt ttcgtgttat agaccgaatt tgtgtatacg
     5821 taaaatttaa tgctattgaa tttttaaaat gaaaaacatg acattaaatt gaattcataa
     5881 tatgtctaat tgactaactt gttggagtca tttactattt tatgtatgac atattttaaa
     5941 aagtgagggt caagcatgtc ttataaag
//
LOCUS       DAKTIP010000157         5932 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAKTIP010000157 DAKTIP010000000
VERSION     DAKTIP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.159"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            214..1152
                     /locus_tag="O0305_002166"
     CDS             214..1152
                     /locus_tag="O0305_002166"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCY0275286.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSEELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAALHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1323..3725)
                     /locus_tag="O0305_002167"
     CDS             complement(1323..3725)
                     /locus_tag="O0305_002167"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCY0275287.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYIPKSFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELDLSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQDKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPILAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3725..4783)
                     /gene="pheS"
                     /locus_tag="O0305_002168"
     CDS             complement(3725..4783)
                     /gene="pheS"
                     /locus_tag="O0305_002168"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCY0275288.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5164..5904)
                     /locus_tag="O0305_002169"
     CDS             complement(5164..5904)
                     /locus_tag="O0305_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCY0275289.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
BASE COUNT         1896 a         1157 c          850 g         2029 t
ORIGIN      
        1 aaataatgta aagaattttc tattcaatac tttatcatgt ttaaattgtg tcactataac
       61 attttcataa acattataca tgaccactat gtattttgta agtatccgca attaattctt
      121 tacaacatac ataaatgttt ctgacgttat tatcatttat gatatgatta tttttgctaa
      181 agacaatgaa attttatgaa aggatttaca caaatggcga atatcgtttt taaattgtcg
      241 gataaagaca taacgacatt aatgtcacgc atttcttttg atactgagaa tttacctcaa
      301 ggtatgaaag cacgtgcaaa gtatcaaaat acaactgtaa atatttacca atcaggtaaa
      361 gtcatgtttc aaggtaatca tgcagaagct gtgtctgaag aacttctacc gcagcattct
      421 caattgaata ctaataaaac taaaaagaaa aatatggcta attcttttct tgaacaaact
      481 ttaatgtatg atcaatttaa ctgtattggt agtgatgagg ctggaagtgg cgattacttt
      541 ggtccgttga ctgtgtgtgc tgctttcgtt accaaagagc atgtccctat tttaaaaact
      601 ttgggtgtag atgattcaaa aaaattaacc gataccaaaa ttgtagaact tgctgagcaa
      661 ttggttactt tcataccaca ttcattatta acactacaca atgaaaaata taatattcag
      721 caagcaaaag gctggactca agtaaaaatg aaagctgccc ttcataatga agccatcaaa
      781 aatgtacttg aaaaaattga ttcgtctcaa ttagactata tcgtcattga tcagttcgca
      841 aaacgcgaag tgtatagcca ttacgcatta tcagatatac cacttccaaa gaaaacaaaa
      901 tttgaaacaa aaggagaatc gaagtcatta gccattgcag tggcaagcat aatttctcga
      961 tacgcatttg ttacatatat ggatcaaata tcaaaaaata ttaatatgac gattcctaaa
     1021 ggtgccggtg ctaaagtcga tgtaatcgcc gccaaaatta taaaaaaata cggtctttca
     1081 cgtttagata ccatttcaaa aaagcatttt aaaaatcgtg aaaaagccca aaaaatttta
     1141 aagcctcttt gatttttaat cattagaggc ttttatgcag ttgtgcgaag catcatctgt
     1201 attccgctgt tcctctttga ctttttaaat gcattggaac caaattggtg aacccaaaaa
     1261 taaaacccca tgttagggat atcaatctac ttcagatatc cttatacatg gggtttaaat
     1321 cattatctaa taacagcacc ttgttcaatt aatgctgctt caatttccgc ttgtactttt
     1381 gaaacgcgct catctgtcaa tgtttcttct gtgtctaaat aatttaaacg tattgcaatt
     1441 gattttttac ctttttctaa atgttcgccc tgatatacat caaagacaag tgtatctttt
     1501 aatatattgc caccatgtgc atgaatcgtt gataataaat cagctgctgg aatattttga
     1561 tctacttcta atgcaatgtc acgagacatg cctgggaatc tcggaattgg ctggtaatta
     1621 atgtaaccta ccgacacagc cattaatgca tcaaaattca actcaaaaac atacgtacgt
     1681 tttaaatcat tatcagctgc taatattgga tgtaattcac caataaaacc aacaacttta
     1741 ttctctaata agatttcagc agtacgacct ggatgtaatc catcaatatc agcacggcga
     1801 taactaaatt caagatttaa cttttcagat actcgatcca cgacaccttt tgctaaatag
     1861 aaatcaaccg tttctttctt atcttgccat tgattgacta cataatctcc agttaaaata
     1921 ccacttaaat attcaacttg atctggtagt tcaccttctc cattagcaaa gaagacattg
     1981 ccgatttcaa ataattttac atctttattt ttgcgtgcca cattatatga tgccgcttcg
     2041 attaaatgtg gtaataaact ttgacgtaat gacgcatgcg cttcactcat tggcatcaat
     2101 aaatcaattg tttgacgctg ttgcatcgaa aatgcagtag catcttcttt agaaactaac
     2161 gaatacgtaa tagcttggtc taatccagca ccttctaaca cttctttaac cattctagtt
     2221 ttatattggc gatcagttag ctgaccacta gtaactttat cgaagacagg taacgttgat
     2281 ggaatatcgt cgtatccata tatacgtgca acttcttcaa ttaaatcttc tttaattgta
     2341 atatctttac gacgtgatgg tactagcact gtaataacat catcatttat ttctgtatca
     2401 aaccctagtt gattaaaaat agtaacaata tcattttgtg acaaatcaaa tccaatagtg
     2461 cgattaattt tatcagcagt gatgtcgatt ggtgtaataa atgcaccaag ttctcctgaa
     2521 gacactctat cttttagcac ttttccgttt gcataagttt gtaataaata acatgcacgg
     2581 tcgactgctt catctacaaa ttcagtagct attccttttt caaaacgact agatgattca
     2641 ctgcgtaaat ttaaacgtct tgatgtatga cgaattgaaa ctggatcaaa aatagcacct
     2701 tcaatcacta tatttgatgt ttgttcttta acttctgaaa aatcgccacc cataacacca
     2761 gctaatgcaa ttggagtttg tccattagta atgacaatat cgctcgttaa taattcacgt
     2821 tctgtatcat ctaatgttgt cattttttcg ccttcattag cttgacgaac aacaatttgt
     2881 tgtgaaccaa tcgcatcttg atcaaacatg tgcaatggtt gaccgtattc taataacaca
     2941 taatttgaaa tgtcaacaac attattaata ggacgtatac ccgcttttat taagcgtgct
     3001 tgcatccaaa ttggcgaggg ttcaattgtc acgtcgtgaa caacacgtgc actataatat
     3061 ggtactttat cttcattttc aattgtcaca gtcagttcat catttgcaga taaatcaagc
     3121 tcatttgatg ttgtctctgg cttagtcatt tttgtattat ataatgctgc aacttcataa
     3181 gcagtaccta tcatacttaa agcatctgca cgattcggcg ttaaatcaaa ttccattact
     3241 tgatcatcta aatataaagc ttgtaaggca tctgttcctg gaacttgggc ttcactaaat
     3301 acataaatgc ctgattcaaa acttttcggt atatagttac ttgaaatacc aatttcttgt
     3361 aacgaacaaa tcataccttc tgaacgttca ccgcgtaatt tggcacgctt aattttaata
     3421 ccaccaggca atctgccacc tactttagca acaatgacat attgtcctgc atcaacgttc
     3481 ggtgcaccac aaacgatttg tacaggttcg tcttctccga tatcaacttg gcaaacattt
     3541 aatttatcag catcaggatg tttctctttt gacttaacga agccgacaac taaatttttg
     3601 atatcttttg tgtagtcaat taaatcatcc acttcaatac ctgtgcgcgt aatacgttct
     3661 gccaaattac ttacagaatc atcgattgtt acatattctt tcaaccattc atttgatatc
     3721 aacattacat gtcacctcta tcttctaccg ctttaaattg atctaaaaat ctcacatcat
     3781 tagtatagaa atgacgaata tcttctatac catatttcaa cattgcaata cggtctggtc
     3841 ccataccaaa tgcaaatcca gagtactctg aagaatcaaa accagccatt tctaatacat
     3901 taggatgtac cattccagca cctaaaattt caatccatcc tgtgtgttta cacacattac
     3961 aaccttttcc tttacattta aaacatgaca catcaacttc tacagaaggt tcagtgaatg
     4021 ggaagtaact tggacgtaaa cgaatttcac gatcagcacc aaataatttc ttagctaaca
     4081 attctaaagt acctttcaaa tcactcattt taacgttttt atcaacaact aatccttcga
     4141 tttgtgtaaa ttgatgacta tgtgtcgcat catcagagtc acgacgatac actttaccag
     4201 ggcaaataat tttaactgga ccttgaccat gacgtgattc catcgtacgt gcctgcactg
     4261 gtgatgtatg cgtacgtaat aaaatttcat ccgtaatata gaaactatct tgcatatcac
     4321 gtgcagggtg tgatttaggt aaattcagca tttcgaagtt ataatgatct tgttcaactt
     4381 catatccatt cacaatttca taacctaaac ctaagaataa gtcttcaatt tcttctattg
     4441 tacgtgttaa tggatgcttt gaaccgattt caatatgacg acctggtaat gatacatcaa
     4501 ttgtttcttc agccaattgc ttatttaatt tttctttaac taacatctgt tgtctttcat
     4561 ctaattcatt ttgaattgtt tgacgcaatt cattcacttt ttgaccaaac gcaggtttat
     4621 cttcattcgg caaatccttc atcaatttca ttagtccgct aactgaccct tttttaccta
     4681 agtatttcac tttaacttct tgcagtgcac gttcatcatt tgcttcatta atatctacaa
     4741 gcgcttgttg ttttaactct gacattgttt gttgttcaga catatgtcac tgacctccct
     4801 attcgaacta ttcattttct atttatcata tttgttgttt aactaaaaaa agactttcat
     4861 cctcttaaaa aataagggac gaaagtcatt tccgtggtac cacccaaatt tatgcctaag
     4921 catacacttg tttaatattg ataacggatt gtctttccga cttaaatctc tttaagtaac
     4981 caaaaaggtg aaaagacttt aaataattgg aataacttgc agtcacgatt attctcccta
     5041 aaacaattat ttatataaat gtccatgcct ttttattggt ttatattcaa ttgtgtatgc
     5101 attcacgtag aaatgttctg ttaaacaatt aattataatt atatcggaaa actttcaaca
     5161 cggtcaacct ttcaaatgat aaagtaaaat actacctgca atcgctacat ttaaactttc
     5221 agctttacca taaataggta tgattaaatt ttgtgtagta tgtgctaata attcaggatt
     5281 aacaccttct ccctcattac ctaatagtaa tgcaaaagaa tcacttgaag taacttcttt
     5341 gtatgccact gcgttttcaa gtgctgtacc ataaacagga ccattaaatt gagtaataaa
     5401 cgtatcgaga tcttgtgtca taactggcaa atggaaaaca ctaccttgac tcgctcgcaa
     5461 cactttatct tgataaggat ctgtcgtacc cttctccatt attacagcat ccattccagc
     5521 agcatccgca gttctaatta atgtgcctaa atttccagga tcttgaacac gatcgatgag
     5581 caatacttgt tgtgctttag aaatatcata atgcggcttc tcaatgattg cgaaaaaccc
     5641 ttgtggtgtc actgtacctg ataaagattc agcaactttc atgtttattt caaaaacttc
     5701 ttgcgcgtat gcgataattt gctgatctaa tcttgccggt tcaattgcaa ataattgtgt
     5761 aattacaatt ccactttgat aagcttcttc aattaaatgc acaccttcaa ttaaagctaa
     5821 tccagtttta tccctctcac gtttcttttt tagcttgttc gcttgtttaa ttctattatt
     5881 ttgtgcagaa gtaatttgtt ccattgatag ctcctcgctt tatttttaaa aa
//
LOCUS       DAKTIP010000158         5900 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAKTIP010000158 DAKTIP010000000
VERSION     DAKTIP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.160"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            110..568
                     /locus_tag="O0305_002170"
     CDS             110..568
                     /locus_tag="O0305_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275290.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            635..868
                     /gene="secG"
                     /locus_tag="O0305_002171"
     CDS             635..868
                     /gene="secG"
                     /locus_tag="O0305_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCY0275291.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            984..1724
                     /locus_tag="O0305_002172"
     CDS             984..1724
                     /locus_tag="O0305_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCY0275292.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPDEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            1758..4130
                     /gene="rnr"
                     /locus_tag="O0305_002173"
     CDS             1758..4130
                     /gene="rnr"
                     /locus_tag="O0305_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCY0275293.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSYRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEGSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPNVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            4152..4616
                     /gene="smpB"
                     /locus_tag="O0305_002174"
     CDS             4152..4616
                     /gene="smpB"
                     /locus_tag="O0305_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCY0275294.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            4707..5068
                     /gene="ssrA"
                     /locus_tag="O0305_002175"
     tmRNA           4707..5068
                     /gene="ssrA"
                     /locus_tag="O0305_002175"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2038 a          818 c         1167 g         1877 t
ORIGIN      
        1 taaatttatg ttgaaatgaa tattgtatga cagggtattc acttttatta aaaggtaaaa
       61 ttaaataaag gttttataga acgtatttaa atatatgagg agtaaacaaa tggctgatag
      121 aacgaataaa gaaattaaaa caggacgctt tattgcaact gcatcaatcg tattctcaat
      181 attattgatt attcattact ttgtttcgtt ggataatgcg actgccaaag cattacttaa
      241 tttaacgaat caaaacactt cagataaagc gattgattac attttaaaca gctttagatt
      301 cactggtatt atgtatattt tggcttatct agcaggcttc atcacttttt ggaatcgaca
      361 tacttatgtg tggtggttta tgtttgcagt ttatgtatca aatagtttgt ttacgttgat
      421 taatttatca atcacaattc aagcaataaa agctgcacac ggtgcgtact taacattgcc
      481 aattttaatt gttattatag gttcggttgc attagcgatt tatatgcttg ttgtttctat
      541 caaacgtaaa agtacattta atcgctagaa aattgatttt aacaataaaa atatgatata
      601 ctacttgtcg tatataagga acggaggaca atttatgcat acatttttaa tcgtattatt
      661 aatcattgat tgtattgcat taataactgt tgtactactc caagaaggta aaagcagtgg
      721 actttcaggt gccatcagtg gtggtgctga gcagttattc ggtaaacaaa aacaacgtgg
      781 cgtcgattta ttcttaaata gattaacaat tattttatca atattatttt ttgtacttat
      841 gatttgcata agttatcttg gtatgtaagg tccggcgatg taaatgtcgg gcttttttat
      901 ttataattaa gaatgtaata gtttaacaat aagctatgta aaatatatag cctagttaag
      961 tatgcaaagg gagcgttaga tttatgcaga taaaattacc aaaacctttc ttttttgagg
     1021 aaggtaaacg tgccgtgtta ttactacatg gttttacagg caattcgtct gatgttcgtc
     1081 aattaggtcg atttttacaa aagaaaggtt atacatcata tgcaccgcaa tatgaaggcc
     1141 acgcggcacc accagatgaa atactgaaat ctagtccttt cgtttggttt aaagatgcgt
     1201 tagatggtta tgattatctt gttgaacaag gttatgatga aattgttgtt gctggtctat
     1261 cattaggtgg ggattttgct ttaaaattaa gcttaaatag agatgtaaag ggtattgtaa
     1321 cgatgtgtgc tcctatgggt ggcaaaactg aaggtgccat ttatgaaggc tttttagaat
     1381 atgcacgcaa ttttaaaaag tatgaaggta aagatcaaga gactattgat aatgaaatgg
     1441 atcattttaa accaactgaa actttaaaag aactaagtga agcattagat acgattaaag
     1501 agcaagttga tgaagtgttg gatcctattt tagtgattca agcagaaaac gacaatatga
     1561 ttgatccaca atccgcaaat tatatatatg accatgtaga ttctgatgac aaaaatatca
     1621 agtggtacag tgaatctgga catgttatta cgattgataa agagaaagaa caagtatttg
     1681 aagatattta tcaattttta gagtcattag actggtcaga ataaaaagag attttaacat
     1741 tagaaaggag gggcataatg aatttaaagc aatctataga agagattatt aatcaacctg
     1801 aatatgaacc tatgtcagtg tcagattttc aagatgcatt aggtttaagc agtgccgact
     1861 cgtttagaga tttaattaag gtgcttgtgg agttagaaca atcaggatta atcgaacgta
     1921 caaaaacaga cagataccaa aaaaagcata gttatagagg tcaatcaaaa ttgataaaag
     1981 gaacgttaag tcaaaataaa aaaggctttg cattcttaag acctgaagat gaggatatgg
     2041 aagatatatt tattcccccg acgaaaatta atcgtgcctt ggatggagat actgttattg
     2101 tagaaatcca tcaatcaaaa ggtgaacata aaggtaaaat cgaaggggaa gttaagtcga
     2161 ttgagaagca ttctgtaact caagttgttg gtacgtatag tgaagctaga cattttggct
     2221 ttgttattcc ggatgataaa cgtattatgc aagatatttt cattcctaaa ggtcaaagtt
     2281 taggcgcagt cgatggtcat aaggtacttg tacaaattac taagtatgct gatggttcag
     2341 ataatccaga aggacatatt tctgctattt taggacataa aaatgatcct ggcgtagata
     2401 ttttatctat tatctatcaa catggcatag aaattgaatt tcctgatgaa gtgttacaag
     2461 aagctgaagc agtacctgat catattgaaa atactgaaat taaaggccgt catgatttac
     2521 gtgatgaatt gacaatcaca attgatggtg ctgatgctaa agacttagat gacgcaatta
     2581 gtgttaaaaa gttagcgaac ggtaatacgc aattaactgt aagtattgct gatgtcagct
     2641 attatgtaac agaaggttct gcattggata aagaggcata tgatagagcg acaagtgtat
     2701 atcttgttga ccgtgtaatt ccaatgattc cacatcgatt aagtaatggt atttgttcat
     2761 tgaatcctaa tgttgatcgt ttaactctaa gctgtcgcat ggaaatcgat gctagtggtc
     2821 gcgttgttaa acatgaaatt tttgatagtg ttatacattc tgattatcga atgacgtatg
     2881 atgcggtaaa tcagattatt actgaaaagg atcctaacat tcgcgaacaa tataatgaaa
     2941 ttacgcctat gctagattta gcacaagatt tatctaatcg tttgattcaa atgagaaaac
     3001 gacgtggtga aatcgatttt gatattagtg aagcaaaagt attagttaac gaagacggta
     3061 taccaacaga tgttcaatta agacaacgtg gcgagggtga acgtctaatt gaatcattta
     3121 tgttaattgc aaatgaaaca gttgctgaac attttagtaa gttagatgta ccttttattt
     3181 accgagtgca tgagcaacct aaatcagatc gcttaagaca attctttgat tttattacaa
     3241 actttggcat catgattaag ggtactggcg aagatattca tccaacaaca cttcaaaagg
     3301 ttcaagaaga agtagaaggt cgacctgaac aaatggtcat ttcaacaatg atgttgcgtt
     3361 caatgcaaca agcgcattat gatgatgtga acttgggaca ttttggctta tcagctgaat
     3421 attatacgca ttttacatca ccaattagac gttatcctga tttaacagtt catcgtttaa
     3481 tccgtaagta tttaattgag aaatcaatgg ataacaaaga agtgaagcgt tgggaagaca
     3541 aattgcctga gttagctgaa catacttcta aacgtgaacg tcgtgctatt gaggcagaac
     3601 gtgatactga tgaattgaaa aaagcagaat atatgattca acatattggt gatgaatttg
     3661 aaggtattgt cagctcagta gctaacttcg gtatgttcat tgaattgcca aatacgatag
     3721 aaggtatggt tcatattgcg aatatgactg atgattatta ccgttttgaa gagcgtcaaa
     3781 tggcattaat tggtgagcgt caagctaaag tatttagaat tggtgacaca gttaaggtta
     3841 aagtgacgca tgttgatgta gatgaacgat taattgattt tcaaattgta ggtatgcctt
     3901 taccgaaaaa tgatcgatca cagcgcccag cgcgaggtaa gacaattcaa gccaaaacgc
     3961 gtggtaaatc attagataaa tcaaaatctg atgataaggg tcgtaagaaa aaaggtaagc
     4021 aacgtaaagg taaaaaccaa cgtaataatg ataaatcagg taatagtaag cataagccat
     4081 tttataaaga taaaagtgtg aaaaagaaag cacgtcgtaa gaaaaaataa gcagcaatga
     4141 ggtgagtatg aatggctaag aagaaatcac caggtacatt agcggaaaat cgtaaggcaa
     4201 gacatgatta taatattgaa gatacgattg aagcgggaat tgtattgcaa ggcacagaaa
     4261 taaaatcaat tcgccgaggt agtgctaacc ttaaagatag ttatgcgcaa gttaaaaacg
     4321 gtgaaatgta tttgaataat atgcatatag caccatacga agaagggaat cgttttaatc
     4381 acgatcctct tcgttctcga aaattattat tgcacaagcg tgaaatcatt aaattgggtg
     4441 atcaaacacg tgagattggt tattcgattg tgccgttaaa gctttatttg aagcatggac
     4501 attgtaaagt attacttggt gttgcacgag gtaagaaaaa atatgataaa cgtcaagctt
     4561 tgaaagaaaa agcagtcaaa cgagatgttg cgcgcgatat gaaagcccgt tattaagcga
     4621 tttagttgct taatcgggct atatttgata tagttatatg tgcttttgta aattacaaaa
     4681 gtatgatttg tttgatttat tatttcgggg acgttcatgg attcgacagg ggtcccccga
     4741 gctcattaag cgtgtcggag ggttgtcttc gtcatcaaca cacacagttt ataataactg
     4801 gcaaatcaaa caataatttc gcagtagctg cctaatcgca ctctgcatcg cctaacagca
     4861 tttcctatgt gctgttaacg cgattcaacc ttaataggat atgctaaaca ctgccgtttg
     4921 aagtctgttt agaagaaact taatcaaact agcatcatgt tggttgttta tcacttttca
     4981 tgatgcgaaa cctatcgata aactacacac gtagaaagat gtgtatcagg acctttggac
     5041 gcgggttcaa atcccgccgt ctccatattt gtagcctaca gcctttgtgg ttgtgggctt
     5101 ttttattttg tgtttttcag gggataatgc attgcagaat ttgttgtgag tattgatata
     5161 gcagtgtttg tataggtgtt tatttgatgg aggaaagagt aataagtgat tatgaattag
     5221 tttttgagat ataaggggac agtgatgtgt gtcaaataag tgtcaaaaaa gttggattct
     5281 gagttttaca ttcaacattg ttcatgaaga aacttcttta tacgcaaaaa attctccatg
     5341 ttatatatgt caatataaaa atgtgaatcg tctacactta attggataaa tggctactga
     5401 aaaagaactt ttcatttttg ttacgtcact aagtgggtgt agttataaag agatgagccg
     5461 agttttgata ttttcattag aatcaatatg cctattaaca caatcagcaa tagttgacga
     5521 gacggaaata aaagaagtcg tagttaagaa atgcatttca caacatacca ttgtagccat
     5581 ttttattgtt ttggatgata aactcttttt ggaattttta gtttttataa tttgcaacta
     5641 cactacttct tttactaata ttaatgtcta agtaatcgat aaaaaatttt ccattgaata
     5701 aatgagaagt taaaaacttt acttaacctt tctcattgca ttttcctatt cacgatttta
     5761 agaacccaac atactacaaa cgaattttaa aaggcgagag taaagcttac ttgtttatta
     5821 tacatattta aaatccaaga gtcagaacag actactcctc tttataacta taaaaaatag
     5881 ctatgaaaaa atctatcgtc
//
LOCUS       DAKTIP010000159         5871 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAKTIP010000159 DAKTIP010000000
VERSION     DAKTIP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.161"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            28..735
                     /gene="splA"
                     /locus_tag="O0305_002176"
     CDS             28..735
                     /gene="splA"
                     /locus_tag="O0305_002176"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplA"
                     /protein_id="HCY0275295.1"
                     /translation="MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITD
                     ATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDV
                     KDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTK
                     YKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFG
                     VYFTPQLKEFIQNNIEK"
     gene            860..1582
                     /gene="splB"
                     /locus_tag="O0305_002177"
     CDS             860..1582
                     /gene="splB"
                     /locus_tag="O0305_002177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCY0275296.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGTTLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKNDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1640..2359
                     /gene="splC"
                     /locus_tag="O0305_002178"
     CDS             1640..2359
                     /gene="splC"
                     /locus_tag="O0305_002178"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCY0275297.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPNGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQAFNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2480..3199
                     /gene="splD"
                     /locus_tag="O0305_002179"
     CDS             2480..3199
                     /gene="splD"
                     /locus_tag="O0305_002179"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplD"
                     /protein_id="HCY0275298.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTVVDGIQQTAKAENSVKLIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVTSDAVVQPGSSGSPILNSKREAIGVMYASDKPTGEST
                     RSFAVYFSPEIKKFIADNLDK"
     gene            complement(3148..3255)
                     /locus_tag="O0305_002180"
     CDS             complement(3148..3255)
                     /locus_tag="O0305_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275299.1"
                     /translation="MLILLLISKNHLSMYGWMIYLSKLSAINFLISGEK"
     gene            3357..4073
                     /gene="splE"
                     /locus_tag="O0305_002181"
     CDS             3357..4073
                     /gene="splE"
                     /locus_tag="O0305_002181"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCY0275300.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4224..4943
                     /gene="splF"
                     /locus_tag="O0305_002182"
     CDS             4224..4943
                     /gene="splF"
                     /locus_tag="O0305_002182"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCY0275301.1"
                     /translation="MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQIT
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFIADNLDK"
     gene            5008..5142
                     /locus_tag="O0305_002183"
     CDS             5008..5142
                     /locus_tag="O0305_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275302.1"
                     /translation="MHYLICIKANQRLIFTNSIVDWFYLCMNEQLFDIIKNINDFESI
                     "
     gene            5306..>5871
                     /locus_tag="O0305_002184"
     CDS             5306..>5871
                     /locus_tag="O0305_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0275303.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         2284 a          862 c         1044 g         1681 t
ORIGIN      
        1 aaaaataaat ttggaaggag gtaaaatatg aataaaaatg taatggttaa aggtttaact
       61 gctttaacta ttttaacatc tcttggtttt gctgaaaata tttctaatca gcctcattca
      121 attgccaaag cagaaaagaa tgtcaaagaa attaccgatg caactaagga accatacaat
      181 tcagtggtag catttgtggg tggtactggt gtagttgttg gtaaaaatac aatcgtaact
      241 aacaaacata tcgctaaaag taatgatatt tttaaaaata gagtatcagc acatcattcg
      301 agtaaaggta aaggcggagg aaactacgac gttaaagaca ttgtagaata tcccggaaaa
      361 gaagaccttg cgatagttca tgttcatgaa acaagtacag aaggtttgaa ttttaataag
      421 aacgttagtt atacaaaatt tgcagacgga gcaaaagtga aagatagaat ttctgttatt
      481 ggttatccaa agggtgcaca aacaaaatat aaaatgtttg aatcgacagg aacgattaac
      541 catatcagtg gaacgtttat ggaatttgat gcgtatgcac aaccaggtaa ttcaggatct
      601 cctgtattga attctaaaca tgaactgatt ggtattttat atgcaggtag tggaaaagat
      661 gaatctgaaa agaatttcgg tgtttatttc acaccacaat taaaagaatt tattcaaaat
      721 aatattgaaa aataagtatc atcaattcat tcgtgaagtt gattttttaa agagtgatta
      781 agaaaacggt tacataatta actaaatata ctgaattatg tatctagata tcaaaataat
      841 taaaagagag gaacttaaaa tgaacaaaaa cgtagtcatc aagagtttag cagcattaac
      901 aattttaaca tctgtaacag gtattggaac aacattggtt gaggaagtac aacaaactgc
      961 caaagcagaa aataatgtca caaaagttaa agatactaat atttttccat atactggtgt
     1021 agttgctttt aaaagtgcaa ctggatttgt agttggaaag aatactattt taacaaataa
     1081 acatgtgtcg aaaaattaca aagtgggcga tcgtattact gcacatccaa atagtgataa
     1141 aggtaatggt ggtatttatt cgattaaaaa gattattaat tatccaggta aagaagatgt
     1201 atcagtcatt caagttgaag agcgtgcaat agaacgtgga ccaaaaggct ttaattttaa
     1261 tgataatgta acgccattca aatatgcggc aggggctaaa gctggtgagc gaattaaagt
     1321 gattggttat ccacacccat acaaaaataa atatgtttta tatgagtcaa ctggccctgt
     1381 gatgtcagta gaaggtagca gtattgtata ttcagcgcat actgaaagcg gaaactctgg
     1441 atcacctgta ttaaacagca acaacgaatt agttggtatt cattttgctt ctgatgtaaa
     1501 aaatgatgat aacagaaatg catatggcgt ctactttaca ccagaaatta aaaaattcat
     1561 tgcagaaaac atagataaat aaacaaattg actttaaacg agcgttgcaa catatctcga
     1621 attgtaaagg agcttgaaaa tgaataaaaa tatagtcatt aaaagcatgg cagcattagc
     1681 cattctaacc tcagtaactg gaataaatgc tgcagtcgtt gaagagacac aacaaatagc
     1741 aaatgcagag aagaatgtta cgcaagttaa agatacaaat atttttccat ataatggcgt
     1801 cgtttcattt aaagatgcga caggttttgt aattggaaaa aatacaatta tcaccaataa
     1861 acatgtatca aaagattata aagttggcga tagaattact gcccatccaa acggtgacaa
     1921 aggaaatggt ggtatatata aaattaaaag catttctgat tatccgggtg atgaagacat
     1981 ctctgtcatg aatattgaag aacaagcagt cgaacgtgga ccaaaaggct ttaattttaa
     2041 tgaaaatgtc caagcattca attttgcgaa agatgctaaa gttgatgaca aaattaaagt
     2101 tattggttac ccattacctg ctcaaaatag ttttaaacag tttgaatcta caggaactat
     2161 aaaaagaatc aaagacaata ttttaaattt tgatgcatac attgaacccg ggaattcagg
     2221 atcaccagtt ctaaattcta acaatgaggt cataggtgtg gtgtatggcg gtattggaaa
     2281 aattggttct gaatataatg gtgccgtata ctttacgcct caaatcaaag attttattca
     2341 aaagcacatt gaacaataaa caaatttaaa tatacaccat gagcatgtgt tcaataattt
     2401 taatgaaaaa catcggtcga atataacata aaaaaacgtc tatatcaaaa gcatcatgaa
     2461 taaacagagg agcacaaaaa tgaataaaaa tataatcatc aaaagtattg cggcattgac
     2521 gattttaaca tcaataactg gtgtcggcac aacagtggtt gatggtattc aacaaacagc
     2581 caaagcagaa aatagtgtga aattaattac caacacgaat gttgcaccat acagtggtgt
     2641 tacatggatg ggcgctggaa caggatttgt agttgggaat catacaatca ttaccaataa
     2701 acatgttact tatcacatga aagtcggtga tgaaatcaaa gcacatccta atggttttta
     2761 taataacggt ggtggacttt ataaagttac taagattgta gattatcctg gtaaagaaga
     2821 tattgcggtc gtacaagttg aagaaaaatc aacgcaacca aaaggtagaa aattcaaaga
     2881 tttcactagc aaatttaata tagcatcaga agctaaagaa aatgaaccta tatcagtcat
     2941 tggttatcca aatcctaatg gaaataaact acaaatgtat gaatcaactg gtaaagtact
     3001 atcagtgaat ggaaatatag tgacatctga tgcggttgtc caacctggca gctctggttc
     3061 acctatatta aatagtaagc gagaagcaat tggtgttatg tatgctagtg ataaaccaac
     3121 aggtgaaagt acaaggtcat ttgctgttta tttctctcct gaaattaaga aatttattgc
     3181 agataattta gataaataaa tcatccatcc atacattgat aaatgatttt tagaaattaa
     3241 caacaaaatc aacaatttta aacatctctg tgattctatt tattcgaaat gatttaaaaa
     3301 ataaaacttc aaaaacctaa ccttatattt atacgaatac ttagaggagc acaaaaatga
     3361 ataaaaatat aatcatcaaa agtattgcag cattgacgat tttaacatca gtgactggcg
     3421 tcggcacaac agtggttgag ggtattcaac aaacggctaa agctgaacat aatgtgaaac
     3481 taatcaaaaa tactaatgta gcaccataca atggtgtcgt ttcgatagga tctggaacag
     3541 gtttcattgt cggtaaaaat acaattgtta ccaacaagca tgtcgttgca ggtatggaaa
     3601 ttggtgcaca tattatagcg catcccaatg gtgaatataa taatggcgga ttttataaag
     3661 ttaaaaaaat tgtccgttat tcaggtcaag aagatattgc cattctacat gtggaagata
     3721 aagctgttca tccaaaaaac aggaatttta aagattacac aggcatttta aaaatagcat
     3781 cagaagctaa agaaaatgaa cgcatttcaa ttgttggcta tccagaacca tatataaata
     3841 aatttcaaat gtatgagtca acaggaaaag tgctgtcagt taaaggcaac atgattatta
     3901 ctgatgcttt cgtagaacca ggcaactcag gttcagctgt atttaacagt aaatacgaag
     3961 ttgtaggtgt tcactttggt ggaaacggcc ctggaaataa aagtacaaaa ggatatggtg
     4021 tttatttctc tcctgaaatt aagaaattca ttgcagataa cacagataaa taaatcctta
     4081 catagataaa tgattttaaa aattaacaac aaactcaaca attcaaatca tctctgtgat
     4141 tccatttatt cgaaatgatt aaaaaaaata aaacttcaaa aagctaacat tataattata
     4201 caaatactta gaggagcaga aaaatgaata aaaatataat catcaaaagt attgcagcat
     4261 tgacgatttt aacatcaata actggtgtcg gcacaacaat ggttgaaggt attcaacaaa
     4321 cagccaaagc cgaaaatact gttaaacaaa ttacaaatac aaatgttgca ccatacagtg
     4381 gtgttacatg gatgggcgct ggaacaggat ttgtagttgg aaatcataca atcattacca
     4441 ataaacatgt tacctatcac atgaaagtcg gtgatgaaat caaagcacat cctaatggtt
     4501 tttataataa cggtggtgga ctttataaag ttactaagat tgtagattat cctggtaaag
     4561 aagatattgc ggttgtacaa gttgaagaaa aatcaacaca accaaaaggt agaaaattca
     4621 aagatttcac tagtaaattt aatatagcat cagaagctaa agaaaatgaa cctatatcag
     4681 tcattggtta tccaaatcct aatggaaata aactacaaat gtatgaatca actggtaaag
     4741 tattatcagt gaatgggaat atagtgtcat cggatgcaat tattcagcct ggtagctctg
     4801 gttcacctat attaaatagt aaacacgaag ctattggtgt aatctatgcc ggtaataagc
     4861 catcaggtga aagcacaaga ggatttgctg tttatttctc tcctgaaatt aagaaattca
     4921 ttgcagataa tttagataaa taattaaaac ttagacattc acccaatcct gacaaaatat
     4981 actataacta acatttatta atatatattg cattatttaa tatgcatcaa agccaatcaa
     5041 cgattgattt tcaccaactc aattgttgat tggttttatt tatgtatgaa tgaacaactt
     5101 tttgacatca ttaagaatat aaatgatttt gaaagcattt gaaagctaca acatttctat
     5161 aaaatttttc aataacaatt gcgccactaa aactcaaaat ttccaccacc aacatccaaa
     5221 ttatcaacat cgcaacataa ccaaatgtta taataaatct attacacaaa gagataaatt
     5281 acttatgcaa aggcggagga atcacatgtc tattactgaa aaacaacgtc agcaacaagc
     5341 tgaattacat aaaaaattat ggtcgattgc gaatgattta agagggaaca tggatgcgag
     5401 tgaattccgt aattacattt taggcttgat tttctatcgc ttcttatctg aaaaagccga
     5461 acaagaatat gcagatgcct tgtcaggtga agacatcacg tatcaagaag catgggcaga
     5521 tgaagaatat cgtgaagact taaaagcaga attaattgat caagtcggtt acttcattga
     5581 gccacaagat ttattcagtg cgatgattcg tgaaattgaa acgcaagatt tcgatataga
     5641 acatctagcg acggcgattc gcaaagttga aacatctaca ttaggtgaag aaagtgaaaa
     5701 tgactttatc gggctgttca gcgatatgga tttgagttca acgcgactag gtaacaatgt
     5761 caaagaacgt actgctttaa tctctaaagt catggttaat cttgacgact taccattcgt
     5821 tcacagtgac atggaaattg atatgttagg tgatgcatat gaattcctaa t
//
LOCUS       DAKTIP010000160         5816 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAKTIP010000160 DAKTIP010000000
VERSION     DAKTIP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.162"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            18..305
                     /locus_tag="O0305_002185"
     CDS             18..305
                     /locus_tag="O0305_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275304.1"
                     /translation="MEKGIFNYDNANVLKLDTNQLNENIKVIDDIFKNYEQIEPTIEV
                     ENGNTKLKLNGYFIASIISPLNLNKLNNLYVEEEFYHTYNELIVKYTEVKE"
     gene            311..1792
                     /locus_tag="O0305_002186"
     CDS             311..1792
                     /locus_tag="O0305_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCY0275305.1"
                     /translation="MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYK
                     GNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEI
                     EESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVD
                     NKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDS
                     KDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKI
                     NGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRID
                     LLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLED
                     FKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNINDMA
                     DKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(1881..2237)
                     /locus_tag="O0305_002187"
     CDS             complement(1881..2237)
                     /locus_tag="O0305_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCY0275306.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            2714..3673
                     /locus_tag="O0305_002188"
     CDS             2714..3673
                     /locus_tag="O0305_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0275307.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIAEKAK"
     gene            complement(3719..3850)
                     /locus_tag="O0305_002189"
     CDS             complement(3719..3850)
                     /locus_tag="O0305_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275308.1"
                     /translation="MRYVTMRQFIKRTVKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(3914..4129)
                     /locus_tag="O0305_002190"
     CDS             complement(3914..4129)
                     /locus_tag="O0305_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCY0275309.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            4294..4845
                     /locus_tag="O0305_002191"
     CDS             4294..4845
                     /locus_tag="O0305_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275310.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEKIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(4897..>5816)
                     /locus_tag="O0305_002192"
     CDS             complement(4897..>5816)
                     /locus_tag="O0305_002192"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCY0275311.1"
                     /translation="YSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGSEDHIKIS
                     LFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELVLRLAIAF
                     YILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSGVFMGMFI
                     IVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLPILLGKNA
                     SLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKNDTPPTMMP
                     AMAAAGKTNTFFGLLYALGIYISALFAGI"
BASE COUNT         2293 a          740 c         1002 g         1781 t
ORIGIN      
        1 ttattaagag gtgcgctatg gaaaagggga ttttcaatta cgataatgcg aatgtattaa
       61 aattagatac taatcagtta aatgaaaaca taaaagtcat tgacgatata tttaaaaatt
      121 atgagcagat agagccaact atagaagtcg aaaatggaaa tacaaaatta aaattaaatg
      181 gatattttat tgcgtcaatt ataagcccat taaatttaaa taaacttaat aatttatatg
      241 ttgaagaaga attttatcat acatataacg aattaattgt taaatatact gaggtaaagg
      301 aataattata atgaaaaaaa tatttatgat ggtacatgag ttagatgtga ataaaggtgg
      361 tatgacctct tcgatgttca atagaagtaa agagttttat gatgcggaca tacctgctga
      421 tattgttact ttcgattaca aaggaaacta tgatgaaatt attaaagctt tgaaaaaaca
      481 aggtaaaatg gatcgaagaa cgaaaatgta taatgtattt gagtatttta aacaaatttc
      541 aaataataaa cattttaagt ctaataaatt gttatataaa catatttcag aaagactaaa
      601 aaatacgatt gaaattgaag agagtaaagg tatttcaaga tattttgata taacgactgg
      661 tacatatatt gcctacatta gaaaaagtaa atctgaaaaa gtgattgatt tctttaaaga
      721 taataaacga attgaacggt ttagttttat agataataaa gtgcatatga aggaaacatt
      781 taatgtagat aataaagttt gttatcaagt attttatgat gaaaagggat acccatatat
      841 ttcaaggaat attaatgcta ataatggtgc tgtaggtaaa acttatgtgt tagttaataa
      901 aaaagaattt aaaaacaatt tagcactgtg tgtttactat ttagaaaaac taataaaaga
      961 ttctaaagat agtattatga tttgtgatgg accagggagt tttccaaaaa tgtttaatac
     1021 aaatcataaa aatgctcaga aatatggcgt tattcatgtt aatcatcatg aaaatttcga
     1081 tgatacgggt gcatttaaaa aaagtgagaa atatattatt gagaatgcga ataaaattaa
     1141 cggtgtaatt gtattaacag aggcacaaag attagatatt cttaatcaat ttgatgtaga
     1201 aaatattttc actattagca attttgttaa gatacataat gctccaaaac attttcaaac
     1261 tgaaaaaatc gtaggtcata tttctagaat ggtaccaacg aagcgaattg atttgcttat
     1321 tgaagtggct gagttagtcg taaaaaaaga taatgctgtt aaatttcata tatatggaga
     1381 aggatctgtc aaagataaaa tagctaaaat gattgaagat aaaaatttag aaagaaatgt
     1441 ttttcttaaa ggatatacaa caactccaca aaaatgcttg gaagatttta aattagtcgt
     1501 ttctacatct caatatgaag gtcaagggtt aagtatgata gaagcaatga tttctaaaag
     1561 gcctgttgtt gcctttgaca tcaaatacgg accaagtgat tttatagaag ataataaaaa
     1621 tggttattta atagaaaacc ataatattaa cgacatggct gataaaatac ttcagcttgt
     1681 taataatgat gtattagcag cggagtttgg ttcgaaagcg agagaaaaca ttatagaaaa
     1741 atattcaacg gaatcaatat tagaaaaatg gttaaatctt ttcaatagct aaatgaaaaa
     1801 atcctaggta ttcatgagta cctaggattt ttacgcatga taaatacaaa aaaagagagg
     1861 atagtcctct caaacaatag ttaataaaat tgtagccatt tcttccagct gtaactacta
     1921 atacaagcta agttcattac gatatgtatg atttcaaatt cataaccact tacatatcct
     1981 cttgtaagtt taagtgtgaa gattgagcct aacataacac aaatataaaa aacattaaca
     2041 taaggcgtta aaaagccgat aataagtgct aaacctccaa caacttcacc aattgacatc
     2101 aatactgcga tggtaggtgg taaacctaaa gagcctaaga agtgaattgt tccatctaaa
     2161 tcaacaaatt tcaaagtacc atgcactaag aatgatgttg ctaacatcca tctaattaaa
     2221 agcataccta atttcatcat gtcacctcca acattcatat ttttaaaaca tagtccaaat
     2281 tatatttgta aacatgtgga aagtaaagat tttttataag attaattatt aaatattcta
     2341 ttgtattgaa caattgttgt agaaagatgt ttaagtcatc agttactttt tgtatttata
     2401 aataaagatt agtacagcat agtgcaaaga ttatagggaa atgaattaac tattgcatcg
     2461 ctatttatga atcgtaaaca ctattatgaa attgcgagag tagtatggac aattagaatg
     2521 atggtttgag ataaatgagt gttattaacc agtgcgtcat tttcaaaatt agatgccatt
     2581 tgctgtttat aataagaaga tttgataatt aactatgata agaaaaaata ataatcccct
     2641 aaatacatta taaacaaatt aaatgataaa ataataatat atatttctac taaagttgtt
     2701 tggaggtaaa tgtgtgaata ggaatatcgt taaactagtt gtgttcatgc taattttagt
     2761 tgtagcagta gcgggttgtg gtcaaaaaga tactgaagag aaaactgaaa tgacgacaat
     2821 aaaagatgaa ttaggaactg aaaaaattaa gaaaaatcct aaacgtgttg ttgtattaga
     2881 atatagtttt gctgattatt tagcagcatt agatatgaaa cctgttggta ttgcagatga
     2941 tggcagcact aaaaatataa caaagtcagt aagagataag attggggcat atgaatcggt
     3001 tggatctaga ccgcaaccga atatggaagt gataagtaaa ttaaaaccgg atttgatcat
     3061 tgcagatgtt agcagacata agaaaatcaa atcagaattg agcaaaattg ctccgacaat
     3121 catgttagtt agcggtacgg gagattataa tgcaaatatt gaagcattta aaacagtcgc
     3181 taaagcagta ggcaaagaga aagaaggcga gaagcgtctg gaaaagcatg ataaaatatt
     3241 agcggagatt agaaagaaaa ttgaacagag tacgttaaaa tctgcatttg cattcggtat
     3301 ctcaagagca ggtatgttta ttaataatga agatacattt atgggacaat tcttaattaa
     3361 aatgggtatt caacctgaag tcacaaaaga caaaactacg catgttggtg aacgcaaggg
     3421 tggtccttat atatatttaa ataatgaaga acttgccaat atcaatccaa aagttatgat
     3481 tttagccact gacggaaaaa cggacaaaaa tagaacgaaa ttcattgatc ctgcagtttg
     3541 gaaatcatta aaagctgtga aagataacaa agtttatgac gttgaccgaa ataagtggtt
     3601 gaaatcaagg gggattatcg caagtgaaag tatggcagaa gatttagaaa aaattgcaga
     3661 aaaagcaaaa taaaaataca gcgctactcg taaatcatat aagagtggcg ctgtattttt
     3721 aattatgttt attatctgtc ggatgtgatg atttacctga aagtttattt cgaataaatt
     3781 taattacata accgacaagg attgttttaa cagttctttt aatgaattgg cgcatcgtta
     3841 catacctcat ttctctatat cttacgaact atatacccat tcatatatgc tttttaaacg
     3901 tcattgtcac aatttaattt ttagggaata taatataacc atctttatct gcttttttag
     3961 taaaaatgac aaaaattgca tgtattattg agatgatggt agggatacct gtccagaaaa
     4021 ataataagtg aaaaagacct tgtccaaatt tatcagcata aaatttatga atacctaaac
     4081 ctccaagaaa taatgcaaca ataacataaa tggctttatt gactttcatt tgtaaatcct
     4141 ccttaactat aattctactt aaattcgttg tgaaaaccaa tatttctaac tttagaattt
     4201 tcaaactttc taaaattata agtatatctt tttaaaataa gctagaattt ctatataata
     4261 aatgttaata acgtaaaagg gaatgatgac atagtgatac gtcaagcacg tccagaggac
     4321 cgatttgata ttgcgaagtt agtttatatg gtttgggatg atatggaatt agaattggta
     4381 aagcatctac ctaaagacat ggtattagat gcaattgaaa aaagctgtgt tgatgcaaca
     4441 tatcgaactt tttatcagca tattttagtt tatgaagtag aaaataaagt agcaggttgt
     4501 attattagct atagtggtga aaatgaattg aaatacgaaa aagcatggga actacttgac
     4561 ttgccagaaa aaataaaaca atatggcacg ccattacctg taaaagaagc taaagacgat
     4621 gagtattata tagaaacaat tgcgacattt gcagcatata gaggtagagg catcgcgaca
     4681 aagttattaa cgtcattact tgaatcaaat acacatgtta aatggagttt gaattgcgat
     4741 attaataatg aagcagcatt aaagttatat aaaaaagtag gctttatatc tgatggacag
     4801 attgaattat acaagcacat gtatcatcat ttaattgtta aataaaaaaa ctcgacagtt
     4861 cgatgtaagt cgattgccga gtagtatcat tttctattaa atgcctgcaa ataatgcact
     4921 aatataaata cctaatgcat ataataaacc gaaaaatgta tttgttttac cagcagcagc
     4981 cattgctggc atcattgtag gcggtgtatc attcttcttg aaacgtctga taactttaac
     5041 aggcattggg aatgataaca acgcaagtaa gtaaaataat gagccaccag gtttaataat
     5101 gatcgtaagt acaataaagg cataagcgat aaagtacatg attgccataa atgttaaaga
     5161 agcattttta cctaatagaa tgggtaaagt tttgcgacca cttgctttat ctttgacacg
     5221 gtcgcgaata ttgttagcca tattaattaa accgatagtg attactatag gtacacttaa
     5281 ccaaattaca taactttgaa tattgccagt ttgaataaag aatgcaataa cgataataaa
     5341 catacccata aatacgcctg agaataattc accgaaaggc gtccatgaaa tagggaaagg
     5401 gccacctgta tataggtaac caacagccat acatactaat ccaactggta ataaccaaaa
     5461 tgaagagtta gcagctaaaa acaaacctaa tattgctgct aagatgtaaa atgcaatggc
     5521 taatcgtagc acaagctctg ggctcatacc gttgcgaaca atggcaccac caatgcctac
     5581 agattcatga tcatcaaggc cttttttata atcatagtat tcattaaaca tattagttgc
     5641 tgcttgaata agtaagcatg ctagtaacat ggcaatgaat aggctgattt taatatgatc
     5701 ttcgctacca agaaaatata ttttagatgc tgctgtacca actaaaacgg gtactacgga
     5761 agcagttaat gtatgaggac gcattaaatg ccaatatttc ttaactgtag aatatt
//
LOCUS       DAKTIP010000161         5790 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAKTIP010000161 DAKTIP010000000
VERSION     DAKTIP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.163"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..980
                     /gene="argF"
                     /locus_tag="O0305_002193"
     CDS             <1..980
                     /gene="argF"
                     /locus_tag="O0305_002193"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCY0275312.1"
                     /translation="NFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALL
                     FEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYDGIEYRGF
                     SQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTYVGDGRNN
                     VANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGS
                     DVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETI
                     IGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE"
     gene            1000..1929
                     /gene="arcC"
                     /locus_tag="O0305_002194"
     CDS             1000..1929
                     /gene="arcC"
                     /locus_tag="O0305_002194"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCY0275313.1"
                     /translation="MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVIS
                     HGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDK
                     QVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVP
                     SPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAA
                     HLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAAL
                     QFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN"
     gene            complement(2029..3026)
                     /locus_tag="O0305_002195"
                     /pseudo
     CDS             complement(2029..3026)
                     /locus_tag="O0305_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3058..3227
                     /locus_tag="O0305_002196"
                     /pseudo
     CDS             3058..3227
                     /locus_tag="O0305_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399387.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
     gene            3834..4184
                     /locus_tag="O0305_002197"
     CDS             3834..4184
                     /locus_tag="O0305_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275314.1"
                     /translation="MKTTTQELKQYMTRLFQLSNNETWECETLEEAAENILPKRFIND
                     SPLAHLILETYTYYNNELHELSIYPFLMYSNNQLISIGYLDHFDMDFLYLTDTKNTII
                     DERHLLKEGGNNHE"
     gene            4271..4582
                     /locus_tag="O0305_002198"
     CDS             4271..4582
                     /locus_tag="O0305_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275315.1"
                     /translation="MKINRYITRGISEQLSLDLQILLWNMVKDRDNQLNTDYLHIFKL
                     QEDDNMLSITHEQEQPAYKLEYHYTNYIKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            4598..5101
                     /locus_tag="O0305_002199"
     CDS             4598..5101
                     /locus_tag="O0305_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCY0275316.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKIWDDKKPACTVITMYLHL
                     DGVLSLDLTTVLILNQLANSEQYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEVMHPLNPKA
                     RQKWTLK"
     gene            5115..5339
                     /locus_tag="O0305_002200"
     CDS             5115..5339
                     /locus_tag="O0305_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275317.1"
                     /translation="MNHDNTIQSNWRTVASCLASQNYISIVKGLVHHFTAIDDEEILD
                     KIYKNFMNDDTITTIFNNNLQAIINHYLSI"
BASE COUNT         2074 a          930 c          962 g         1824 t
ORIGIN      
        1 gaaacttttt aactttgtta gacttcacac aaaaagaaat ggaattttta cttaatttat
       61 ctgaagatct taaacgcgca aaatatgcag gaatagaaca acaaaaaatg aaaggtaaaa
      121 atatcgctct actttttgaa aaagattcaa cacgcactcg atgtgcattt gaaacagcgg
      181 cttatgatca aggtgcacat gtaacatacc ttgggccaac aggttctcaa atgggtaaaa
      241 aagagtctac caaagatact gctcgtgttt taggtggaat gtatgatggc atagaatatc
      301 gtggattctc acaaagagta gttgaggatt tggcaaaata ttctggcgtt cccgtatgga
      361 atggtttaac agatgaagat catcctacac aagtacttgc tgatttttta acagctaaag
      421 aagtattgaa aaaaccatat aatgaaatta acttcacata tgttggagat ggacgtaata
      481 acgttgcaaa tgccttaatg caaggagcag caatcatggg catgactttc catcttgtat
      541 gtcctaaaga actaaatcca actgatgaat tattaaatcg gtgtaatgat atagctgaca
      601 aaaacggtgg tgaaatcctt ataactgatg atattgatga aggtgtcaaa ggatctgatg
      661 taatttacac agatgtttgg gtatctatgg gtgaacctga tgaagtttgg gaaaaacgta
      721 tcaaattatt agaaccatac cgtgtaacta aagaattaat gaaaaaaaca ggtaatccac
      781 atacaatttt tgaacattgc ttaccatcat tccatgatac tgaaacaata atcggtaaac
      841 aaattcaaga aaaatatggt ctaactgaaa tggaagtaac aaatgaagta tttgaaagtg
      901 aacaatcagt tgttttccaa gaagcagaaa atagagcaca tacaataaaa gcagtcatgg
      961 tagcaacatt aggagagtaa atgaaaggag gatataaata tgtctaaaat cgtcgtagct
     1021 ttgggtggta acgctttagg acaatcgcct aaagaacaat tagatttatt aaaaagtaca
     1081 tctaaatctc tagtaagttt aatcgataaa ggatacgaaa ttgtaattag tcatggtaat
     1141 ggtccacaag ttggaagtat taacttaggt ttaaattatg cggcggaaca caaacaaggt
     1201 cctccttttc ctttccctga atgtggtgct atgagtcaag cttatattgg ctatcagatg
     1261 caagaaagtt tacaaaacga acttcattca atgggcatag ataaacaagt tgttacgcta
     1321 gtaacacaag ttcaagttgc aagcgatgat tctgctttca ataaccctac taaaccaatt
     1381 ggattatttt atacaaaaga gcaggctgat aaatttacga aagaaaaagg ttatactttt
     1441 gtagaagatt ctggacgcgg ctatcgtcgc gttgtccctt ccccacaacc tataagtata
     1501 gttgagttag atagtataga aacattaatc actcatggaa cactagttat cgctgccggc
     1561 ggtggtggaa ttccagtaat taaggaaaat gaagtttata caggtgttga tgcagttatt
     1621 gataaagata aaacgagtgc tttattagca gcacatttac aatctgatca attaatcata
     1681 ttaactgctg tggaccatgt ttacattaac tatggaaaag aaaaccaaag aggtctcgat
     1741 gaagtgtctg tggatgaaat gaaaaaacat atctctgatg gtcaatttgc taaaggaagt
     1801 atgcttccaa aagttgaagc tgcacttcaa tttcttgaaa aaaatactaa aggcagtgtt
     1861 ttgattacat ctctagcagg attaggggac gctttagacg gtaaaatagg aacattaatt
     1921 aagaattgaa attataatac tttagttcaa ttacctttat aaacaaaaga gcctataatc
     1981 ctagggcact gaacccttaa aacgggaact taataaaaac accatgttct aggctattaa
     2041 ttctctgtat tttacagggg ataagtagcc tagtttttgt tgaattcgat tattattatt
     2101 attattgttt ttaatgtact tttcgacaat atctattaca atgtgattag agtgattaag
     2161 ctcattattg atgtaaaaag tttcagactt tagcgaggaa tggaaacttt ctatcggggc
     2221 gttatcggca ggtgttccct ttcgggacat acttctgata atgccttttt cttcgcacaa
     2281 ttgataataa gcataagatg tataaacgct gccttgatca ctatgtaata tacaaccctc
     2341 aggtatatcg atttgattta atgtatcatt aactaaactt tggtcttgtt tatcatctat
     2401 tttatacgcc acaatttctc cgttataaat atccattatc gaagataaat acaacataga
     2461 atgaccaaat ggtaaataag taatatcggt tgttaatact tccattggac aactcgcttt
     2521 aaattgtctt tgtaataaat tgtccgtttt ataatacggt ttacctattt ttgtcatctt
     2581 tttaggtcta actcggcagt tcaaatgatg cttctgcatc attctctgta ctctcttatg
     2641 attaattggt gatgtataac attgattaat caatgctgta atcttacgat aaccgtaggt
     2701 atagtggtta gctttacata attcaataac tttttgtgtt acggtatcat ttttatgggt
     2761 tttgtttttc catcggtaat atgttgattt aggtatgttt aatactttta gtagcaattt
     2821 gattgaatat tttactttta attgatccac taaatctatg actactgttg gtaccacttc
     2881 ctttccaata ccttgtactt ttttaaaata tccaattcta tatcttttct cttattttct
     2941 aattttaatt gttctacttc tgacagctct tctaatcctt taccgtaggt atattgttta
     3001 ccaacttgtt gtgaaaatct atatgctagt tttggtaaaa ctgaggaagg acgctggata
     3061 gctaagaatg cccataatta tggatttatt ataagatatc ctaaaaacaa agaaagcatc
     3121 acaggatatc aatatgaacc ttggcattta cgttatttag gaaaaagaag gcgacaaaag
     3181 tgtacaaatc tggtgaaaca ttagagggat ttgtaggctt aaaatgatca atttaaaata
     3241 atcgaaactt caactacctt attatcgacg aagtgtattc cagcacttcg tctttatttt
     3301 taccgcaaac attatacctt actatttaat aaatctattt tatatctaca tacttcctct
     3361 ttctttttct aaaaattttt gagaattctc tttatcaatt tcaataacac ttatctaatg
     3421 aaacagtcat tgtcgtaatt ttttcataac tacacgaggc ttatattgaa attgctaaag
     3481 aacatcccac gatgattttt tatcgggaaa ggaatgggat ttgcagatta gaagaagcag
     3541 aaggattgtg caagcacaat cccaaaatcc cgttattctt tataaataaa taatatatat
     3601 aaggaagcag agaccccacc ctatcatcaa tacttataaa taatgagttg attgttcgtc
     3661 aaaataggcg gaatttcggg atttccttgt cataccaagg tttgtagcgg ttttttatat
     3721 ggaattttct catattgtca acgggatcag taataaatga caaatcaaat atatattatc
     3781 tcaatgatgt atatgaagta catcactatc acactatcta gggaggcaat gatatgaaaa
     3841 caactactca agaactcaaa caatatatga ctcgcttatt ccaattatct aacaatgaaa
     3901 catgggaatg tgaaacactg gaggaagcgg cagaaaatat tctacccaaa cgttttatta
     3961 atgattcccc acttgcacat cttatacttg aaacttatac ctactataat aatgagctac
     4021 atgaactaag tatctatcca tttctaatgt attctaataa ccaactcatc agtattggtt
     4081 atctggatca ttttgatatg gactttctat acctcacaga tactaaaaat acgattatcg
     4141 atgaacgtca tttactaaaa gaaggaggga ataaccatga ataattggat caaagtggca
     4201 caaatatctg ttacagtcat taacgaagtg attgacatca tgaaagaaaa gcaaaatgga
     4261 ggaaaatagt atgaaaatca atcgatacat cacaagaggc attagtgaac aactatctct
     4321 agaccttcaa atcttacttt ggaacatggt aaaagatcga gacaatcaac ttaatacaga
     4381 ttacctacac attttcaaac tacaagaaga tgataatatg ttgtcaatta cacatgaaca
     4441 agaacaaccc gcatacaaat tggaatatca ctatacaaac tatataaaaa atcaaaatgc
     4501 attacctaag aaagtctacg tcatccgaga agatgatgta gacgcttttt attatgtgat
     4561 gcttttgcca gaagaatact aaaggaagtg acgatatatg aatacaatca aaagtacgat
     4621 acacacagaa gcgatattta gcgatgatga acaacatcgc tacttactca aaaagatatg
     4681 ggatgacaag aaaccggctt gtactgtgat aacgatgtat cttcatttag atggtgtatt
     4741 atcactcgat cttactacgg ttctcatcct caatcaatta gctaattctg agcaatatgg
     4801 cgctgtttat ctcgttaatc ttttctctaa tattaaaaca ccagagaacc ttaaacatat
     4861 caaagagcct tatgatgagc acacagatat acacttaatg aaagcaatta gtgaaagtga
     4921 cacagtcatt cttgcttatg gtgcctatgc gaagcgacca gttgttatcg accgtgtcga
     4981 acaagtgatg gaaatgttaa aacctcataa aaagaaagta aaaaagctca taaatccagt
     5041 aacgaacgaa gttatgcatc cactcaaccc taaagcacgt caaaaatgga cactaaagta
     5101 aaggagtatt atctatgaac catgacaaca ctatacaatc caattggcga acggttgcta
     5161 gttgtttagc atcacaaaat tacatttcaa tagtaaaagg tctggtgcat catttcacag
     5221 ccatcgatga cgaggaaata cttgataaaa tctataaaaa ttttatgaat gatgatacta
     5281 taacaacgat atttaacaat aatttacagg caattattaa ccattaccta tcaatatgaa
     5341 tattaaataa aactagaacc tccttcactt agcactgagg aatattctta cattttttaa
     5401 taagtggtga tataaaaaag cagttagtgg ccatagattc aatttctaag gtagcttccc
     5461 tttcaataat ttttgaattc aaaattttaa tataaatccc ttccttttat attatacaca
     5521 atctattttt tagttttatt tgtgatacgc ttctgcttat cagttaatga tgcggttttt
     5581 gaaaaataac tacagaaatt tatagaagaa caacaaaact caaacgctat atcatataat
     5641 aagttcttag aagcttacaa agtagacacg tcttctgatt aaactaatac agttgatgaa
     5701 ttaacagaaa atccagtatg tactgagtac ataagagatc gaacagaagc tagagaaaaa
     5761 ggtcaaggta gaacttatta agcatattaa
//
LOCUS       DAKTIP010000162         5753 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAKTIP010000162 DAKTIP010000000
VERSION     DAKTIP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.164"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            55..786
                     /locus_tag="O0305_002201"
     CDS             55..786
                     /locus_tag="O0305_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCY0275318.1"
                     /translation="MSTLKLEFKKSISNKIIYTLVVLFTFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFMCPTEIINIVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(946..1566)
                     /gene="srtA"
                     /locus_tag="O0305_002202"
     CDS             complement(946..1566)
                     /gene="srtA"
                     /locus_tag="O0305_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCY0275319.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVGVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
     gene            1760..2251
                     /locus_tag="O0305_002203"
     CDS             1760..2251
                     /locus_tag="O0305_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0275320.1"
                     /translation="MIRYAKKEDLNAILAIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNRWLDLAFYELD
                     LQD"
     gene            complement(2689..2859)
                     /locus_tag="O0305_002204"
     CDS             complement(2689..2859)
                     /locus_tag="O0305_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275321.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(2921..3019)
                     /locus_tag="O0305_002205"
     CDS             complement(2921..3019)
                     /locus_tag="O0305_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275322.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVERFMENAY"
     gene            complement(3038..3937)
                     /gene="sdaAA"
                     /locus_tag="O0305_002206"
     CDS             complement(3038..3937)
                     /gene="sdaAA"
                     /locus_tag="O0305_002206"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCY0275323.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(3951..4631)
                     /gene="sdaAB"
                     /locus_tag="O0305_002207"
     CDS             complement(3951..4631)
                     /gene="sdaAB"
                     /locus_tag="O0305_002207"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCY0275324.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(4634..5677)
                     /locus_tag="O0305_002208"
     CDS             complement(4634..5677)
                     /locus_tag="O0305_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCY0275325.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         1770 a         1108 c          871 g         2004 t
ORIGIN      
        1 agagtaccaa tcaaaagaag aagtcataaa tgattacatt aatttataat tgaggtgtcc
       61 actttgaaat tagaatttaa aaaaagtatt tccaataaaa ttatttacac gcttgttgtg
      121 ctattcacat tccttttctt acttgggtat tttttaccaa taggtataga caaagttaaa
      181 agtttaagct atagccagtt tttcttcagt tcatatactg ttgcaacgca attagggttc
      241 ttactatttt catttgttat tgcatatttt attaataaag agtattcgaa taaaaatatc
      301 ctgttttata aattgattgg cgataatatc tttaccttct tctataaaaa ggtagcagtg
      361 ttattttttg aatgtttagt gttcataatt ctaagtatca ctattatttc aattatatat
      421 tcggatttct cacattactt actattaata atcttatttt cactcgttat cttacaatat
      481 atcttagttg ttggaacgat tagtatggtt tcacctaaca ttttaatcag cttaggtatc
      541 agcattgtat attggattgg ttcagtcatc ttagttgcca ttaataaaaa tatatttggt
      601 atcgttgctc catttgaggc gagcaatacg atgtatcgtg ccgtagaaaa aatattgaat
      661 aacgagtcaa catttatgtg tcctactgaa attataaata tagtatcatt cttcgttctt
      721 ctatttatag ttaatactat tgtcttatta ctaagtagaa agagatggtt gaaaatcggt
      781 atgtagtata aaaaaggagc ggtatacatt gccgcttctt tttattatgt atttttacaa
      841 tctccaccaa tgacacgtcg tcattgctac ctcataccaa acaaaaaaca gtctcgcaat
      901 caagactgtt ttccactcaa tatattcatc cattagcgta atagattatt tgacttctgt
      961 agctacaaag attttacgtt tttcccaaac gcctgtcttt tcattgtaat catcacaagt
     1021 aattaatgtt aattgtttat ctttaccttt ttgttcatct agaactccta catctgtagg
     1081 cttaacatct cttatacttg tcattttata cttacgtgtt tcattaccaa ctttaaagta
     1141 caccatacta ccttttttgg ctgctttaag atttgtaaat tgatagttcg gacggtcaat
     1201 gaaagtgtgt cctgcaattg aaatattttg atcatctagt gattcatttt cttctgcaaa
     1261 gcttacacct ctatttaatt gttcaggtgt tgctggtcct ggatatactg gttctttaat
     1321 atcagcatct ggaatttcaa tatagcctgc cactttcgat ttatctttcg gaatttgagg
     1381 tttagcttgc tgctttttat ctttactcgc ctgttctttt acatttttat catattgttc
     1441 aatcttttca tctttatctt tatcgtgaag ataattatcg atatgtggtt tagcaaacaa
     1501 atatgctgcc actaggataa gtaccacacc agcgattgtc attaatcgat ttgtccattt
     1561 tttcatacgt taaggctcct tttatacatt tcacaatgcc tgttaacatt tcatatagta
     1621 taccattaat ttcaaaatga ctcatagcaa ttcattttat actataaaat ttacatgtat
     1681 acttttacgt tagatttcat tacacatatt tgcattcaaa taacgaaacg cttttaataa
     1741 ttactaaggg ggaattgata tgattagata cgctaaaaaa gaggatttaa acgctatatt
     1801 agcgatatac aatgatgcca ttatcaatac tacagctgtt tatacttatg aaccacaaac
     1861 catagacgaa cgtgtcgcat ggtttgaaac gaaacaacgt aagcatgagc ctatctttgt
     1921 atttgaggaa aatggaagtg tcttagggtt tgcgacgttc ggttcattta gaccttggcc
     1981 agcataccta tatacaatcg aacattctat ttatgtcgat gcttcagcta gaggaaaagg
     2041 tattgctagt caattactac accatttaat tgtggaagca aaagctaaag gttatcgtgc
     2101 gctagttgca ggcattgatg cttccaacaa agcgagtatt cagttgcatc aaaagtttgc
     2161 ttttaagcat gccggcacac tgaccaatgt aggttttaaa tttaatagat ggttagattt
     2221 agcattttac gaattagatt tacaagacta gtaatgtttg aatcacataa tataaacaag
     2281 acaaccatgt taattccctt aacataacaa gccaacatat aaaattttaa acttctcagg
     2341 ggagtgggac agaaatgata aagagccact aatgatttat tatgtagtgg ttcttacaca
     2401 ttagccacag ctaatgtgta cttaaaaata ggaatacatg agtaaaactc atgcataaga
     2461 aatactaatt tctatagaaa aagtatttct ttatcgtcgt cccaccccaa ctcgcacatt
     2521 attgtaagct gacttttcgt cagcttctgt gttggggccc aaaaagcttg ttacaagcgc
     2581 attttcgttc agtcaactac tgccaatata actttgtaga gcatattaca ttgatttaca
     2641 ttgtcccttt tatttattct tttcaaatac tatccccata gctttgattt aacgcttttt
     2701 ctcaataaca aaacgaatat agtagaacat gaaaacgata atcatgctga gcgataaaga
     2761 tttaaataat agattgaccc acgttccctc agtcgtatat ccatatgtaa tcgttgtgtt
     2821 aatgatgaat gctataaaga tgattgatag tcttagcata tcatcactcc ttttaagtta
     2881 ttttagatat acgggggcgc ttttgcaatc actattttga ttagtatgca ttttccataa
     2941 atctttcaac ttcttcagag ataattaaga agcatctatc tggtactaat gatccagaca
     3001 gatgcttctt ttttatcaat attttattgt tatctcatta attattttta accatatctt
     3061 cagctgtgcc aaagatttta cgtttaattg cttcgccagt tggtgtgcct gctagtccac
     3121 ccaatccagt ttcacgtaat gatgcaggaa ggttacgacc aaccttatcc attgcttcaa
     3181 taacttcatc aacagggatt ctactttcaa tacctgctaa tgctaaatct gctgaaatta
     3241 aagcgttacc cgaaccaatt gcatttctca taacacaagg aatttcaaca agtccggcta
     3301 ctggatcaca aactaaacct aataaattac ttatcgctaa tgccatagcg tgcccggatg
     3361 cttctggtga tcctccgaat atagctactg ctgcagctgc ggccattgca gatgctgaac
     3421 caacttcagc ttggcagcca cctgttgcac cagctacact tgcattgttt gctacgacac
     3481 gcccaaacaa tgctgaagtg aataagaaat caatcatttg ctcttctgtt aaatcatgtg
     3541 ttttttctaa tttaaaaagt gcaccgggaa tggtacccga ggaaccagct gttggcgttg
     3601 cacaaataat acccatcgca gcattgactt catttgttgc aatggcacct ttgactgcgt
     3661 caatcatttc atatccagac aaagcatgat gtgtttcatt ataatcacgt agtttagcag
     3721 catcatgacc agtgtagccc gttacacttt caaccccatc acctgtcgtc cctttgatta
     3781 ctgcgtctcg catgacatct aaattttgtt tcatttgcgc tcgcacttca tcacgtgatt
     3841 taccgcttaa ttccatttct tctttaacca tgatatccgc aaatgacata ttattttcta
     3901 cggcataatc tatagtctct ctaattgaat caaacatgtt tattccccct ctaatttata
     3961 taggaaacgt ttacgtcact gtatttctct ttaattgtat ttaatgttga ttctgagatt
     4021 gctttattta atggtattac aaccaagcat ttatcttcat ctatcttaat aaattcatct
     4081 ttacagtcta atttcatatc gttgatatca ttgatgaaat gatttacttg tgctttagtc
     4141 atatttccgt caacaactaa aattggtaat ccatgattta aatctacttc tagtccattt
     4201 atatgaatac ctttaatttt aattgtacca ccaccgattg aaataccgat aatttcaatg
     4261 tagcgaccat cattacgaga tgatttgata taagcacagt ttggatgttg accaatacta
     4321 tcgccttctt cttcgatgat atctatttta ataccatcat cagctgcaat ttctaatgaa
     4381 gatttaattc ggttatcaaa tgttgaatat cccattgctc cacccacaat agcgacatct
     4441 gtaccatgtc cttggtgtgt ttgagcaaat gattcataat aatgtatttc aatattttta
     4501 ggaatatctc ccaatattgc gcgtgctgaa ttcccaatct ttactgcacc agccgtatga
     4561 gaacttgaag ggcccatcat aactggtccg ataatatcga aagcactttg ataatcatag
     4621 ctctttgcca taattaaaca ctctccttaa tatgattctt tttgctcggc attttaaagt
     4681 tgatattcat taaattaaac ttattaatta gtgttccaat aatataggct aagacaatgc
     4741 tgacaataat cactgttgcg attgttacaa ttgatgtcac tgcattatta aagccaaaca
     4801 atacgatggc gcctgcaatt ggtgttgcca tacctttgac acctattact agtccgctaa
     4861 atgtcacgat acatgcgttg acgaccccaa tcagtgcatt tgtaccatat agttgtactg
     4921 gatattgcgc tattaaatca atttgcgtca atggctcaat acaaactgca aatgcttttg
     4981 acggtccacc aatgtttaat tttcggaata aaataaggtt aacaaatgag ctacctgtac
     5041 atgttagtgc tccaatagcc ataggaacac ctgtcagtcc taataaactt gttaatacca
     5101 ttgaacttag cggtgtcata cctgtaacag gaatcactag tcctaaaatg accgctaatg
     5161 catatggatt gttatcacct accgcagtga cagcactacc tatttgtttt aatgttgcta
     5221 gcacaccagg tgtaatgatt gatgcaagtc cgaaagcaat tgctggtgca aataagatca
     5281 ccacaattaa gtccaagcct tctggaactt tcttttcaat ccatttaatt aaaaaagcta
     5341 cgccataagc tgcgatgaat gctggtaata atttaaagtc atgtaatact aaaccaacaa
     5401 tgaccgcaaa tactggtgca acgcctaagt ttaagcacgt tagaatacct actgcgatac
     5461 cgcttaaact tcctgctaaa tccccaatat cttgtagaaa tttaatatca aatacgccac
     5521 caatagcata acttaagaat gcttgtggta gaaatgtcgc acaagctgca ccggataatg
     5581 cttgtagtcc ttgtttaccg tacggtgcat actttaaaaa tagcgtcatg atcactaaaa
     5641 ccaagactaa tgtgcctaca cctaacagaa tatccatttc ccaaaaacct ctctctatgt
     5701 ttattttatt ttcagaccat aaacatcgta cacccttaag aaaacgtttt caa
//
LOCUS       DAKTIP010000163         5744 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAKTIP010000163 DAKTIP010000000
VERSION     DAKTIP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.165"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1429
                     /locus_tag="O0305_002209"
     CDS             <1..1429
                     /locus_tag="O0305_002209"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCY0275326.1"
                     /translation="IVRESKIDPDNIVGIGIDFTSSTIIFTDENLNPVHNLKQFKNNP
                     HAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPKIMEVMNRAPEIM
                     EKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFDKIDPKLSKVIQD
                     KVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGIGSEKDKEMTMVM
                     GTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFEYVAKQAPKSYVD
                     EAENRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLTGCIFGLTLQTKH
                     EDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMMDIYANVLNKKLI
                     VMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAEKVQRYETLFKAY
                     KALHDIHGYKKANIMKDIQSLRVEG"
     gene            1643..2608
                     /locus_tag="O0305_002210"
     CDS             1643..2608
                     /locus_tag="O0305_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCY0275327.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
     gene            2943..4019
                     /locus_tag="O0305_002211"
     CDS             2943..4019
                     /locus_tag="O0305_002211"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCY0275328.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            4269..4952
                     /locus_tag="O0305_002212"
     CDS             4269..4952
                     /locus_tag="O0305_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0275329.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(5068..5730)
                     /locus_tag="O0305_002213"
     CDS             complement(5068..5730)
                     /locus_tag="O0305_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCY0275330.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
BASE COUNT         2075 a          768 c         1103 g         1798 t
ORIGIN      
        1 tatagtacgt gaatcaaaaa tagatccaga caatatagta ggtattggta tagactttac
       61 ttcatctact attattttta ctgacgaaaa ccttaacccg gtacataact taaaacaatt
      121 taaaaacaat ccacatgcgt atgtgaaact ttggaaacat catggtgcat ataaagaggc
      181 agagaaatta tatcaaactg ctattgaaaa taataataag tggttaggcc attatggata
      241 taatgttagt agtgaatgga tgattcccaa aataatggag gtcatgaatc gagcaccaga
      301 aattatggaa aaaacggctt atattatgga agcgggcgat tggattgtaa ataaattaac
      361 taataaaaat gtacgctcga attgtggatt aggtttcaaa gcattttggg aagaagaaac
      421 agggtttcat tatgatttat ttgataaaat agaccccaaa ttatcaaaag taattcaaga
      481 taaagtatct gcaccggttg ttaatattgg tgaagcagta gggaaactgg atgataaaat
      541 ggcacagaaa ttaggattat caaaagaaac tatggtaagt ccttttatta ttgatgccca
      601 tgctagttta ttaggtattg ggtctgaaaa agataaagaa atgactatgg tgatgggaac
      661 aagcacatgc catcttatgt taaatgaaaa gcaacatcaa gtgccaggta tatcaggttc
      721 tgtaaaagga gcaattattc cagaattatt tgcttatgaa gcggggcaat cagcagtagg
      781 tgatttgttt gagtatgtcg ctaagcaagc accaaagtca tatgtagatg aagcagaaaa
      841 tagaaatatg actgtatttg aattaatgaa tgaaaagata aaacatcaaa tgccaggtga
      901 aagtgggctc attgctcttg attggcataa tggaaatcga agtgtattaa gtgatagcaa
      961 tttaacaggt tgtatctttg gattaacttt acaaactaag catgaggata tttatagagc
     1021 atatttagaa gctacagcat ttggtactaa gatgattatg caacagtatc aagattggca
     1081 tatggaagta gaaaaggtat ttgcatgtgg cggtatacct aaaaagaatg ctgttatgat
     1141 ggatatctat gcgaatgtac tgaataaaaa actaattgtt atggatagtg agtatgcacc
     1201 agcaataggc gcagcaatat taggtgcagt cagtggtggc gcacataatt caattaatga
     1261 cgcagttgat gctatgaaag agccaatttt atacgaaatt aatccagaag cggaaaaagt
     1321 acaaaggtat gaaacattat ttaaagctta taaggcttta catgatatcc atggttataa
     1381 aaaagctaat ataatgaaag atatccagag tttaagagtt gagggataaa aaatttaatt
     1441 tgacagtaat tagcaataat aaacgctata aattactaaa atcaattaat acaactagaa
     1501 tttccattta cagaaatatc agcaacatgt acatgtcata tatacaagaa agcgctttta
     1561 tattataatt attatgaaaa tgaatattat gtccgcttgt gatggactat agctatatag
     1621 aagagacaaa ggagatattt ctatgaaaaa aattatgatt actggtgcat taggacaaat
     1681 tggtacagaa ttagttgtta agtgcagaga aatttatggg acagataatg ttcttgctac
     1741 agatattagg gaacctgaag cagactcacc tgtacaaaat ggaccatttg aaatcttaga
     1801 cgtaacagat cgtgaccgta tgtttgagtt agttagggac tttgaagcgg atagtctaat
     1861 gcatatggca gcattattat cagcaactgc tgagaaaaat ccaattctag cttgggattt
     1921 aaatatgggt ggattaatga atgcattaga agctgcaaga acttataatt tgcacttttt
     1981 cacaccaagt tcaattggtg catttggaga ctcaactcct aaagttaata cgccacaagt
     2041 aacgattcag caacctacga caatgtatgg tgtaaataaa gtagctggag aattattgtg
     2101 tcaatactat ttcaaacgtt ttggtgtaga tacaagaagt gttagattcc caggtttaat
     2161 ctcgcatgtt aaagagccag gtggcggtac tacagactat gctgttgaaa tatacttcaa
     2221 agcagtaaga gagggtcatt atacaagctt catagataaa ggcacgtata tggatatgat
     2281 gtatatggat gatgcaattg aagcaattat taaacttatg gaagcagacg acgctaaatt
     2341 agaaactaga aatggttata atttgagcgc aatgagtttt gatccagaga tggtaaaaga
     2401 agcaattcaa gaatactatc ccaattttac attagattac gatgttgatc ctattagaca
     2461 aggtatcgct aatagttggc cggattctat tgatacaagc tgttcacgtg gcgaatgggg
     2521 atttgatcct aaatatgatt tagcgagcat gactaaatta atgttagaag ctattgaaca
     2581 aaaagatact gttaaaaata ataactaatc atttccattc actttaatac acggaatgat
     2641 attttaaatt actctttatt ttaataaact agtgcatgaa ttctaatatt attcattata
     2701 cttattgaat tcgcgagctt agttcatttt aaagtaaaga gttttttgta tgtaaagtat
     2761 attagtaaaa cacagattac ttgtctctgt gaaattaata cgtttatact aaaagatata
     2821 gtctttctag aaatctgtta attaattttg aatttttaga aaatttgttg aacagcaaaa
     2881 tatggattgt tataatttaa gttaaacaaa ttcttataca atattattag gaggcaatca
     2941 ccatgtcaca agcagttaaa gttgaacgac gagaaacatt aaaacaaaaa ccaaatacat
     3001 ctcaactagg ttttggtaaa tattttactg attatatgtt gagttatgat tatgatgcag
     3061 ataaaggatg gcatgatttg aagatagtac cttatggtcc tattgaaatt tcacctgctg
     3121 cacaaggtgt tcattatggt caatcggtat tcgaaggatt aaaagcatat aaaagagatg
     3181 gggaagttgc acttttccgt cctgaagaaa attttaagcg tcttaataac tcgttagcac
     3241 gattagaaat gcctcaagta gacgaagcag aattgttaga ggggctaaaa caattagttg
     3301 atattgaaag agattggatt cctgaagggg aaggtcaatc attatatatt cgtccatttg
     3361 tttttgcaac agaaggggca cttggcgttg gtgcatcaca tcagtataaa ttattaatta
     3421 ttttatctcc ttcaggtgca tattatggtg gtgaaacttt aaaaccaact aaaatctatg
     3481 tagaagatga atatgtgcgt gctgttcgtg gcggtgtagg ctttgcaaaa gttgcaggta
     3541 actatgcggc aagtttatta gcacaaacta atgcaaataa attaggttat gaccaagtat
     3601 tatggcttga tggtgttgaa cagaaatata tcgaagaagt tggtagcatg aacattttct
     3661 tcgttgaaaa tggcaaagta attacaccag agttgaatgg cagtatttta cctggtatta
     3721 cacgtaaatc tattatcgaa ttagctaaaa acttaggata tgaagtcgaa gagcgccgcg
     3781 tttcaatcga tgaattattc gaatcatatg ataaaggtga gttaacagaa gtatttggta
     3841 gtggtactgc agcagttatt tcacctgtgg gtacattgag atacgaagat cgtgaaatcg
     3901 ttattaataa taatgagact ggtgaaatta ctcaaaaatt atacgacgtc tatactggta
     3961 ttcaaaatgg tactttagaa gataaaaatg gttggagagt cgttgtacca aaatattaat
     4021 aaaaattgaa tataatcatg aaaacaatat gcaaaagtta atcatgacta aaatcccttt
     4081 caatgaagcg aagcatggta ataaattaag tttacaatgt ttattgttaa gtattgaagg
     4141 ggatttcact tattattata tttaattcaa tatttaaata gaacaatact acgagttcat
     4201 tttcagggga taacgaatat catagaatca gcataatacg aaataaataa ataggagtat
     4261 tgatatcaat ggaatggata ttatttgata aagatggtac gttaattgaa tttgatagaa
     4321 gttgggaaaa aataggggta cgatttgtac aatcattgct tgagactttc ccagtacata
     4381 ataaagaagc tgctttaaga caactcggtg tcattaaaga atctattgat ccaaaatcag
     4441 tgatgggttc aggatcttta caacaaatta tccaggcatt taatgatgtg acgggacaag
     4501 atacaaccga ctggtccaag tcaacaagtc aaaagctggt agatgaacgt attcctgaaa
     4561 ttaattgggt agaaggtgtt aaagaagcac ttatcgattt gaaagcaaaa ggctatcaac
     4621 ttggtattgt tacgagtgat actaaaaaag gtgtagaaca atttttagca cataccaatg
     4681 ctacctcgtt gttcgatttg atcatttcta ccgaagcgga tgcctatgag aagccaaatc
     4741 ctaaagtatt atcgccttta tttgagcaat ataatgtaga tcctcagaaa gtagctatag
     4801 taggagacac tgctaatgat atgaagacag caagtaatgc aaatttaggt atggcaatag
     4861 gtgtattaac aggtattgca acaaaagaag aattacatga agctgatatt attttaaata
     4921 gtgcggcaga tattttagaa gctttaaatt aaaaagaaag acatagtgta tatgtgaatt
     4981 aatgtattcg atagaattga taaaataatc gataatatct atggaatata cattgaatat
     5041 aaacatatac gctatgtctt tagtctttta tcgtgtatct acttgtcgat atgtttgaat
     5101 aattcgagca attttgttta tcataggatt taaagattcg gggtccttat ggatatcata
     5161 ttcattaata ttgatacgta caactggaca tgcattaaag ctattaatcc aatcgtcata
     5221 gcgtttaaat agctttttcc agtattcagg gtctgtatta atttccattt cgcgaccacg
     5281 ttcaataata cgatcaatga cctcatcata gttacattct aaataaatca ttacatcagg
     5341 tttaggaaaa taaggtgtca tgaccatggc attaaataag tctgaatatg ttttgaaatc
     5401 ttctttactc attgtgcctt cttcttcatg catttttgca aaaatatcaa catcttcata
     5461 aattgatcga tcttggacaa agccaccacc atattcaaac atacgctttt gttctttaaa
     5521 acgttcagct aagaagtaaa tttgcaaatg gaaactccat cgttcaaaat cgctgtaaaa
     5581 tttatctaaa tatggattat gttcgacatt ttcaaaagac gttttaaagt ttaatttatc
     5641 tgcaagtgct tgcgttagtg ttgattttcc aacaccaact gtacctgcaa tggttataat
     5701 ggcattttgt ggaataccgt aattattcat tggtaatatc tcct
//
LOCUS       DAKTIP010000164         5719 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAKTIP010000164 DAKTIP010000000
VERSION     DAKTIP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.166"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(214..735)
                     /locus_tag="O0305_002214"
     CDS             complement(214..735)
                     /locus_tag="O0305_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCY0275331.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(763..2058)
                     /locus_tag="O0305_002215"
     CDS             complement(763..2058)
                     /locus_tag="O0305_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCY0275332.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2098..2574)
                     /locus_tag="O0305_002216"
     CDS             complement(2098..2574)
                     /locus_tag="O0305_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275333.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2743..3279)
                     /locus_tag="O0305_002217"
     CDS             complement(2743..3279)
                     /locus_tag="O0305_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCY0275334.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3490..4428)
                     /locus_tag="O0305_002218"
     CDS             complement(3490..4428)
                     /locus_tag="O0305_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0275335.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDNNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            complement(4698..5324)
                     /locus_tag="O0305_002219"
     CDS             complement(4698..5324)
                     /locus_tag="O0305_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0275336.1"
                     /translation="MLLCESKIINKNPKYRIIKYNDEYLMVDIISTWISLFFPFINWF
                     IPKEYVKISREEFENLNIVKPAKKNVFWPVAGSSALLGVALRKYTHLLDIQLDKKLVI
                     AICCITFIGILIFYVRLIKKSSLNIYNTKNKRSKIFLIPTLKNVCFTLFGYILFGGLT
                     MLFLDALLSMSYQNIIVYFVWIAVIMGFFLVNIALIIDKNIHVILKNQ"
     gene            complement(5348..5593)
                     /locus_tag="O0305_002220"
     CDS             complement(5348..5593)
                     /locus_tag="O0305_002220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275337.1"
                     /translation="MYYLKLTRWKNDGLAGFTAGSISAILVYWTNQKNEFGIKDKNDW
                     IGHKLDVGIDAVEKSAEKTVDGVENVMVKLQKVFLII"
     gene            complement(5559..>5719)
                     /locus_tag="O0305_002221"
     CDS             complement(5559..>5719)
                     /locus_tag="O0305_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275338.1"
                     /translation="IMALSMLLTLNPQNIIGFIGWLVMTAGFFLLNMSSIIDKKIYVL
                     SKTNTVEK"
BASE COUNT         1858 a         1047 c          750 g         2064 t
ORIGIN      
        1 ataatgtgaa ttaattcaca aagtatatag ctatgatcaa aaaaatgtgt cagttattca
       61 aattttttat taactacaga ttaatttaat ttcaaaaaaa ttaaaaaaac tcccaatcaa
      121 tctaagacat tattttcacc tcacgattta cgtcgtcatt gagaaatgaa tcatatcttt
      181 atagattaat tgggaaattt tagtttctaa tatttaatta aatgcttgat gtaaagcatc
      241 agaagaatgc ttccacatac cttcatcatg gttcattaaa aagtctttta aaatagcttt
      301 attctcgtca ccaatttgat ctatgataat atgatgtttc attgatttat ccatcatatt
      361 aacatgttca ggcatacttt tataaccacg acgaattgct ttattcacta ataaatagca
      421 cgctgttaaa ccagcgtaat atggtccttg ttcattacgt tctgttgtta cccaacacaa
      481 ccaatattct ttagcttcat cgccttcaac agcagcttta tcagtaatcc atttcacacc
      541 tttttcaact ttggcacgag catgcatgcc tccaatatca ataaatgctt ctttgttagc
      601 aacatcgata aaaactggtg caatattgtc taagctaatt gaaccaatat ttgtaccttt
      661 atgaccatct agagggtcat tttttatgat attaaaatta aagccttttt tctcagacaa
      721 tgatataacc tccatattta acgataacaa ttttagccct gtttaattaa gctcattaat
      781 caaattttca atagcttctc taattgtctt atctttaatc tttgcaacca tttctctagg
      841 ataatataat ttatagtctt ggcgattaat gcctgtaata ctatgaatga ctaatgattg
      901 attgcttatt tctctaatcg taccatcttg tcttaataaa tgaatcggtt tgcgatttga
      961 ccccggtcga tcatagtcat atggcaaatc agaaaatgct tcactcacaa aataataatc
     1021 tggattaata ccacctgctt caaacagttc ttgcagttct gatattgtaa taattgagcc
     1081 atcaaatgga atatatttaa ataagtctcg attaataaat cgacttgcta aatcacttaa
     1141 aatagcatca tcttcttttg tccatttttc caaataatac gtaactaccg cttcatcgag
     1201 ttcaacatat tgttcaatcg taactgtctc ttcaaaaaat ggaataaaat catgtggatg
     1261 caacttaaat tcatagcctt cattataaag ctgttttgcg cgtttcaaac aattattaag
     1321 cagcacttct ccaccacgac taactgggtg gaagtaaatt tgccaataca tttgataacg
     1381 actcataata aagttttcaa ctgcatgcat accactttct ttgattagta cttcatcttt
     1441 agaaggtcgc attaatctta aaatacgctc catatcaaaa gcaccatatg atacacctgt
     1501 gaaatacgca tcacgttgta aataatccat tctatccgca tcgatttgtg acgaaatcat
     1561 cgaaatgacc aatttattat gatgcgtttt attaattact tccgcaactt ctcttggaaa
     1621 ctcaggcgcc actttacgta atacagcatt cacctcagta tctccagtaa taatcgcttg
     1681 tgtgtatgct tcatggtctg tattaaatat tttttcaaaa ctatgtgaaa atggaccatg
     1741 tcctaaatca tgtaataatg cagcacataa tgccaacggt ctatctttat tgtcccatgc
     1801 atcatgacca ataaatgact catcaattaa tcgtctaact atttcataca cacctaaaga
     1861 atgtccaaag cgactatgtt ctgctgtgtg aaaagatagg tacagtgttc ctagttgtct
     1921 aattcgacgt aacctttgga attccttagt tttaattaaa tcccatatca attgatcttc
     1981 aacatgaata tatcgatgta tcgggtcttt aaaaactttt tcttctacta attttaaatc
     2041 tacatatgcg ttagtcatta ttcccctcct tttcgtttaa tataatattt aatttactta
     2101 aaatgctttg tacataagtg ctaagtctaa cttttcgcca tacatttctg gctcataaga
     2161 gcgtaagatt gtaaaacctt gctctttata gtaagctact gcttcttcat ttttattatc
     2221 tacttctaag taaacacctt caaatttatc ttcaaaacgt gataatcctt catttaacaa
     2281 tgctgtacca taacctgtat gttgcgattc tggtttaaca taatgagctg ataaatataa
     2341 ttcttcaccg taaataaagt tagcaaagcc aacgatgtca ttaccttctt caacgactaa
     2401 gaataattgt tcttgaagtc ttttctttaa atgatgttca ttatatgaag cttctaacaa
     2461 gtgattaact gttgtcgcag cgtatatatt taagtatgta ttaaaccaag ctttagttgc
     2521 gacatctcta atttgaacaa catctttttc agttgcttgt cttaccttga acatgacttt
     2581 ctccccttat taacaagttt taataacggc attataccac aacttgctca atacttaata
     2641 aacaatgatt gtctattcaa tttatatatt tatattttcc gttaaaatta aaaataaaaa
     2701 ataacgaagc aaaaaatcac ttcgtttagt atgaggtatg tcttattgca atatactatt
     2761 ccactcagtt gcacgtgcta aggcatagtt gtctttcatg atgtcaccag gcttttcagc
     2821 agttccaata atataaccat ttaaagtggc acctaaaaag tctaaactat atttcatttg
     2881 cgtaattgct ggcttcgctt ttatttttgg acaatctcca ccaactaaaa taactctaaa
     2941 atccttttcg gccatttgtg ccttaaaatt aggatatcgt ttatcttgta atgtttctga
     3001 ccaatgttcg ataaatgctt tcaatggtgc tgaaatgcta taccaataca ctggtgatgc
     3061 aaaaataatt gtatcactag ccaatatttt atctagaatc ggcaaatagt catcgtcata
     3121 tgaagtaata gtctctgctg tatgtctcac gtcacgtatc ggtttaaact gatgttgtgt
     3181 cacgtcaatc cattgatact ctaaatcttg caaagcgaat tttgttaatt gtgcagtatt
     3241 accgtttggt ctactcccac caaacaaaac agtaatcatt ttagcctaac ctcacttttg
     3301 attaataaat atctgtgttt ttcgttacct aattatacta tcataagctt tgcctaccga
     3361 atagtaaaac gcttacaact tttatataaa tttgacgaaa tttcgtcatg ccttatataa
     3421 cgtcgtttgt gatacggggc taattcatga tgaaattaga tacatatatc accattaaat
     3481 acaattcatt tagtcttcaa tcggaaacag ttcatcgata tattgaatct catcatctga
     3541 taaaacgata tctgcagctt taatattttc aacgacttgt tctgcacgtt ttgcaccagg
     3601 aataatcaca tcgatagctg gtctcgttaa ataaaatgct aatacaatgt tcgcaattga
     3661 agtttgatgt gctgcagcta tgctttccaa agcttttacg cgacgcacat tttcttcaaa
     3721 tacacctggt ttaaaatcac gacgtgtagt acgatggtca ctaaatttag tgttctcatc
     3781 atattttcca gctaaaatac cggatgctaa tgggaaatat ggaataaatg tgatttggtg
     3841 atcaacacaa tattgtaata ctgcctcatt ttcgcgatgc aataaattat attctaactg
     3901 tacaacatca acgtaaccat ctttatttgc ttctttaagt tgatctaatg tgaaatttga
     3961 tacaccaatt gctttaatct tcccttgttc cttaagctct tgtaatgctg caactgcttg
     4021 atctttcgga gtgttgttat ccggaaaatg aatataatat aaatcgatat aatcagtttg
     4081 tagacgtttc aaactattct caacttgttg ttttaaatat tccggttgat tgttctgatg
     4141 tacttcttga ttttcatcaa attcatgaga ccctttcgta gcaattttaa tttgctctcg
     4201 cggatattct ttaacaactt ctccaaccaa ttcttctgat cgttctggcc cataaatata
     4261 tgccgtatct aataaattaa taccatgatt aatggcttga cgaacaacat cttttccttg
     4321 ttcttcatct aagttcggat ataaattatg cccacctact gcgttcgtcc caagtgcgat
     4381 tggaaacact tcaacatcag atttacctaa gtttacaaat tgcttcatta gacccagctc
     4441 cttatcttta taacttttcc actatcacac tatcatgatt taatacaact tccaaatgtt
     4501 agaattagag cttgaattta agcggtgtaa taggaaaacg ttatataaaa ctcagttaaa
     4561 ataagtattg cttaaattga tatgcatata gcgcgattgc tcgttgacct ttaaattaca
     4621 tgtacttttg aatcagaaat tgaaaaacat ttaagaacga caatattaaa gccattgcaa
     4681 atttaatttg gtaatttcta ttggttttta agtatgacat gaatgttttt atctataatt
     4741 aaagctatat taactagaaa aaaacccatt ataactgcaa tccaaacaaa atatactatt
     4801 atgttttgat aactcattga taatagtgca tctaggaata gcatagtcaa tccgccaaat
     4861 aaaatatatc caaataatgt gaaacaaaca ttttttagtg taggtattaa aaaaattttt
     4921 gaccttttat ttttagtatt ataaatattt aaagatgatt ttttaattag gcgtacataa
     4981 aatattaaaa tccctataaa tgtgatgcaa catatggcaa taactaattt tttatcaagt
     5041 tgaatgtcaa gtaaatgtgt atattttctt aatgcaactc ccagcaaagc agagctacct
     5101 gcaactggcc aaaaaacatt ctttttagca ggtttaacaa tatttaagtt ttcaaactct
     5161 tctctactaa ttttgacgta ttcttttggg atgaaccaat taataaaagg aaaaaataaa
     5221 ctaatccaag tgcttattat atcgaccatt aagtattcat cattatattt aataattcta
     5281 tattttgggt ttttattgat gattttagat tcgcatagca acatttacca gctccatttc
     5341 ttagggctta tatgattaga aatacttttt gaagcttcac catgacattt tcaacaccat
     5401 ctactgtttt ttctgcagat ttttctacag catctatacc aacgtctagt ttatgtccta
     5461 tccaatcgtt tttatctttt attccaaatt catttttttg attggtccaa tatacaagta
     5521 ttgccgaaat agaacctgca gtaaatccag ctaaaccatc atttttccac cgtgttagtt
     5581 ttagataata cataaatttt tttgtcaata atcgatgaca tgtttaacag aaagaaacct
     5641 gcagtcatta ccaaccaacc aataaagcct attatatttt gaggatttaa agttaataac
     5701 atacttagag ccattattg
//
LOCUS       DAKTIP010000165         5695 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAKTIP010000165 DAKTIP010000000
VERSION     DAKTIP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.167"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            2..1093
                     /locus_tag="O0305_002222"
     CDS             2..1093
                     /locus_tag="O0305_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0275339.1"
                     /translation="MKFLKDTSIAEISSIVYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKN
                     NVKKTATNLFILTMIICTIITYLLYNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKE
                     IAEKNRHINTLIAEQERHRIGQDLHDTLGHVFASLSLKSELAYKLIDADVEKVKAELL
                     AINKLSRESLNKVREIIDDVKLPSFIEEIDSIRKVLKDADIDFTFENKELAQVLSPTK
                     QSMLVMITREAINNVIKHANASKVHGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQ
                     RVQHLNGTLAVDSTNGTKIIIEISTGGIA"
     gene            1090..1800
                     /locus_tag="O0305_002223"
     CDS             1090..1800
                     /locus_tag="O0305_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCY0275340.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVFLDIEMPGMTGLEILADTDNGLDAMKLIEEYNPNVVFLDIEMPGMTGLEVL
                     AEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYVLKERSIEELVETINKVNNGEKE
                     YSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEISEKLFLTDGTVRNYTSVIIDKL
                     FADNRFDAWKKANEKGWI"
     gene            complement(1913..2101)
                     /locus_tag="O0305_002224"
     CDS             complement(1913..2101)
                     /locus_tag="O0305_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCY0275341.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            2240..2773
                     /locus_tag="O0305_002225"
     CDS             2240..2773
                     /locus_tag="O0305_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0275342.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(2916..3770)
                     /locus_tag="O0305_002226"
     CDS             complement(2916..3770)
                     /locus_tag="O0305_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275343.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            4101..4676
                     /locus_tag="O0305_002227"
     CDS             4101..4676
                     /locus_tag="O0305_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275344.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(4730..>5695)
                     /locus_tag="O0305_002228"
     CDS             complement(4730..>5695)
                     /locus_tag="O0305_002228"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0275345.1"
                     /translation="YISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFF
                     GVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEI
                     TYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVI
                     SGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQ
                     IQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINL
                     KMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN"
BASE COUNT         2044 a          761 c          904 g         1986 t
ORIGIN      
        1 tatgaaattt ttaaaagata cttcaattgc tgaaatatcg tctatagttt atctgatttt
       61 tcctattgcc ggtatatttt ttaatgaagt atatggtccc aaatggttgt atattatatc
      121 agtcattgtc ttttcgttgt cgtatcttat attagttata gtaaataata gacttaatac
      181 attaatgttt tacattttgt tgattattca ttattttatt atttgttatt ttgttttcag
      241 tgtacatcca atgctaagtt tgtttttctt ttatagtgct tttgccgttc catttacttt
      301 taaaaataat gttaaaaaaa cggcaactaa tcttttcata ctaacaatga ttatatgtac
      361 aataataacg tacttattgt ataacaacta ttttgttgca atgatggttt attatgtcgt
      421 tatatcgtta ataatgctag ataattttaa aaaaatgaaa aaccgtgaat atcaaaaaga
      481 aatagcagaa aaaaatagac atattaatac attaattgct gaacaagagc gacatagaat
      541 tggtcaagac ttacatgata cgttagggca tgtgtttgca agtttatcat taaaatcaga
      601 attagcttat aaactaatag atgctgatgt agaaaaagta aaagctgaat tattagcaat
      661 taataaatta tctcgtgaat cattgaacaa agttcgagaa attattgatg atgtaaaatt
      721 accatcattt attgaagaga ttgatagtat acgtaaagtt ttaaaagatg ctgatattga
      781 ttttacattt gaaaataaag aattagcgca agtattaagt cctactaaac aatctatgtt
      841 agttatgatt acgcgtgaag cgataaataa tgttattaaa catgcaaatg cttcaaaagt
      901 tcatggtaaa ttaaaaactg taaacaatca taaattactg cttatgattg aagatgatgg
      961 caaaggtatc gatagtgatt gtgaggtgaa aagtatttca cagcgtgtac aacatttaaa
     1021 tggaacttta gcagtcgact caacaaatgg aactaaaata atcattgaaa tctcaacagg
     1081 aggaatagca tgacatcttt aattattgca gaagatcaaa atatgttacg acaggcaatg
     1141 gttcaattaa ttaaactaca tggtgatttt gaaattttag cagatactga taatggtctc
     1201 gatgcaatga aacttattga agaatataat cctaacgttg tttttttaga tatagaaatg
     1261 ccaggcatga ctggacttga aattttagca gatactgata atggtctcga tgcaatgaaa
     1321 cttattgaag aatataatcc taacgttgtt tttttagata tagaaatgcc aggcatgact
     1381 ggacttgaag ttttagcgga aattagaaaa aagcatttga atattaaagt gattattgta
     1441 acaactttta aaagaccggg atactttgaa aaagcagttg tgaatgatgt ggatgcatat
     1501 gttttaaaag aacgttctat agaagaattg gtggaaacca ttaataaagt aaataacgga
     1561 gagaaagaat atagcgccac attgatgact tcattttttg tagataaaaa cccattaacg
     1621 cccaaagaac aaattgtatt aagggaaatt ggcaatggtt taagtagtaa agaaataagt
     1681 gaaaaattat ttttgacaga tggaacagtt agaaattata catctgttat aattgataaa
     1741 ttatttgcag ataatcgttt tgatgcttgg aaaaaggcaa atgaaaaagg ctggatctaa
     1801 atacaaacaa aaaagtattg agtgttttga tttaagagac ttggaaagtg agtcaataat
     1861 caaaatctta ctcaatactt ttattttggt actcgcaatt tagatttgtt aactaatttt
     1921 ttcgcttatt ataaaacgaa acaatcaagt aaacgataaa gcctacaaag atacccaata
     1981 aaatagatag tactgccgtc actattaatg gtaatttgaa aaatatttgt aggaaaatac
     2041 caatgataat tgcgataatt actgcaatta atgtgactgt attttcattt gaaatgttca
     2101 tttattttca ctccttaaca ataacattat atcatgctat agctttccaa aatattgaaa
     2161 tatgtagata tggctattga cgatatttct taacttttat atgattaatc ggaatgaaaa
     2221 aagagaagta ggtggcaata tgaagtcaaa taaatcgctt gctatgattg tggtagccat
     2281 cattattgta ggtgtattag catttcaatt tatgaatcat acgggtcctt tcaaaaaggg
     2341 gacgaatcat gaaactgtac aagatttaaa tggtaaagat aaagtacatg ttcaaagagt
     2401 tgtggatggt gatacattta ttgcaaatca aaatggtaaa gaaattaaag ttaggcttat
     2461 aggggttgat acgccagaaa cggtgaaacc gaatacgcct gtacaaccat ttggcaaaga
     2521 agcatcaaat tatagtaaga agacattaac aaatcaagat gtttatttag aatatgataa
     2581 agaaaaacaa gatcgctatg gtagaacatt ggcgtatgta tggataagta aagatcgtat
     2641 gtacaataag gaattagtgg aaaagggact tgctagagag aagtattttt caccaaatgg
     2701 caaatataga aatgtattta tagaagcaca aaataaagct aaacaacaga aattaaatat
     2761 ttggagtaaa taattaaata ggtgattata tatattaaag taaaatatac ttgaagcatc
     2821 gatttattat taccataaat ttatatatag aaataattta aaggcataat aaccatagtg
     2881 cgtatcgagc gtaatggatt attatgcctt tgattttact tgaatatagc tgtgatttga
     2941 tcaacctgtt ctttatttaa agggtgttta gatagctcta tggcttcgct aataatacgt
     3001 cgtaacgaag attttgttga aactgtatca acatatttat ctctttcttc agaaccttga
     3061 atgatattaa ctgtaccatc actaaagtaa ataacaccgg tactttggac tttaaagtta
     3121 aagttttgtt caatatgatc ctttaatgct ttcgcattat ttgctgctaa ttgatacgga
     3181 tcataatcat aaaaatcata aataacacga tttggttgaa ctgtttctgt aaaagtatag
     3241 atagactttc ttgatgaatt aaactgatca tgatattgct ggctaatgta atgaccgaca
     3301 actttttcaa tattactatt gcttatttct gtatcatgct catcaggtac atcaaagtga
     3361 taaaatgttt tttcacccca gctttttaca tcaacgttta ttaaaccgat atctgaaaca
     3421 atgataaaat caaatgaacg tgcatactcg aaaaacggat gtttagtagc taaattgctt
     3481 gtaacgataa tatcgtaata ttttaattta ccattttcga gatagctatc taaaatcttg
     3541 cgtaattctc ttttagcatt tttgtatgca tggtggccaa catcattatt agaactcagc
     3601 atttgacttc ttaattcagc attttctgca cttaatgctt tgttcttttt aataagttgc
     3661 tttcttgcat aaacttcggt atctatttta ctattactat acctttgatt taaaactaat
     3721 ataccaatta atgctacaat gataatgata agtacaacat aaaaagacat tttttcacca
     3781 atcctttttg acttctttaa ctttgtatac aataataatt aataaagatt aattgttatt
     3841 caatttccca catttttatt agttgatttt agttcatcat tgttataatc aaattataaa
     3901 ctgacagata ttgatgttca atgaatatga cgtgaaagat tcgtgaattc aagtttatgt
     3961 cgaatttatg ttataacggt catttaaatg acagaattag gtcactcata gtattttgaa
     4021 gattgaattc attaatttta aaatgtataa tgatatttgt gaaagcgctt gcttaggagg
     4081 tgtatttgag agtgaatgaa atgaatgcta aagaacaatt agtggacaat ttaatgaaaa
     4141 catcatcgca attatttaaa tttcacggtg aagttgccat gcagcttttc ttaaatgatg
     4201 aattaaaatt accttctatt gttgaaatat gcgtggaacg taagcgttta agtgatattg
     4261 tgaaagttat tccgcaatca tatgcgttac tatacataga taagcaagat caagcaatag
     4321 ctaaagaaga tttatcactt tcaaaaattg caaaagttta tgtgcaatat gatgatacaa
     4381 caataatgag tattttcgtt tatgatgtag taaacgatga atggattttt agattggatc
     4441 cgaatatacg tatacctaag agtaacatat acttccatag tttaaattgg gatgtggatt
     4501 atattaaacc ggagatcgtt ctaatgtatg atctaatgca acaccatcag tatcatcatt
     4561 attccaatta taaacgagtc atagatgcat taagctacta tcaatttttt attttaaaat
     4621 ttgtagtagg tgagcaacgt attaaggatg caatccagag aacaataaat aattaagaaa
     4681 aagcaattca taacgcagtt gaatacatgt gttacgaatt gcttttatat tagtttttat
     4741 cacacaagtt ttttaatgca accccgtgat agcaaaactc atatgtagat aatacagctt
     4801 tttcagcatc atctacatga attccaaaca tcatcgagat ttccgaagca ccttggttaa
     4861 tcatttttaa gttaattttt gattcagcta aggcatgtgt aattttattt gcagtaccga
     4921 tgactttatt cataccttcg ccgacaatca ttaatattgc taaatcatgc tcaatactta
     4981 gctcatcaac atcacatttt tgacgaattt catttaatac ttttgtttct ttattttgaa
     5041 tttgttttga acgcataacg atactgatag tatcaatacc tgaaggcata tgatcaaatg
     5101 aaatattatt atcctctaag acacctaata tctttctagt aaagccgact tgtctattca
     5161 ttaaatactt tttgatatta ataacggtaa aatctttatc acaacttata ccgctaatca
     5221 catttttcgc atttatttct ctatcatgca ctataaatgt acccttatct tgagggcggt
     5281 tcgtattttt aatcaccaca gggatgcgat ctttataaag tggttgtaag gcttcatcat
     5341 ggaaaacact aaaaccagca taagataatt cacgcatttc tcgataagtg atttcttcga
     5401 ttaattcagg atctttgatg atatttggat tagctttata aataccagac acatcggtga
     5461 aattttcata aattgtggct ctaacaccac ttgatatgat ggcgccagtt atatctgatc
     5521 cgccacgtgg aaatgtaact atatatcctt catgagatac gccaaaaaat cctgggataa
     5581 ttagtttttc atcataatct cttaattttt taatttcaga gtaagcacta tctaatattt
     5641 gtgcttcttg tgggacgtca gtaacaaaaa tacccgcttc cttcggtgat atata
//
LOCUS       DAKTIP010000166         5673 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAKTIP010000166 DAKTIP010000000
VERSION     DAKTIP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.168"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(160..435)
                     /locus_tag="O0305_002229"
     CDS             complement(160..435)
                     /locus_tag="O0305_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0275346.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(442..906)
                     /locus_tag="O0305_002230"
     CDS             complement(442..906)
                     /locus_tag="O0305_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCY0275347.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            1053..1253
                     /locus_tag="O0305_002231"
     CDS             1053..1253
                     /locus_tag="O0305_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCY0275348.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHE"
     gene            1256..2512
                     /locus_tag="O0305_002232"
     CDS             1256..2512
                     /locus_tag="O0305_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCY0275349.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHEIKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAGKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            2602..3132
                     /locus_tag="O0305_002233"
     CDS             2602..3132
                     /locus_tag="O0305_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCY0275350.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(3392..4543)
                     /gene="yfkAB"
                     /locus_tag="O0305_002234"
     CDS             complement(3392..4543)
                     /gene="yfkAB"
                     /locus_tag="O0305_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCY0275351.1"
                     /translation="MNSVKLKQPVSIYNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            4767..4928
                     /locus_tag="O0305_002235"
     CDS             4767..4928
                     /locus_tag="O0305_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275352.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            5059..5574
                     /locus_tag="O0305_002236"
     CDS             5059..5574
                     /locus_tag="O0305_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0275353.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
BASE COUNT         1960 a          846 c          993 g         1874 t
ORIGIN      
        1 tgatttaaat ttttcatagg ctattttgat ttaaaattaa aacaaaggca atactgaaac
       61 aatgcagttt ggccaatcat tgtattatca tttaataatg ataactatga aaggcgtaca
      121 ccaaattgtt aagtattgcc ttataaaaac aaaatcatct tagcgttgat tttttctatt
      181 aacaaatgtt tctttcgcat cctttaatga acgttctaat tcaggattat tacgacgaat
      241 ttcttcaaca acatctttcc agtataaaac ttcgtcttta atcttgctga ctttagaagg
      301 cttacgagtg cggttaccat tttttgcatc tttaactgat tgaactaaag cttgacgtgt
      361 agatttatca gctaaactaa ttgcaccacc aattacggca ccaattaaaa taccaggaac
      421 aaatttattt tccataaaaa actacccctc tttcaaattt gcatctttta cgatgtagtc
      481 tattaaatta tcacaagatg ataataccat gtcgtataca ccttcaaaat tattcgtgta
      541 gtatggatct ggtacatcac tctcttccat attactaaat tctaacagtt tgaacaattg
      601 tcccttaaga ttaggattga tagatttaat attatcaacg ttactttgat ccatagccac
      661 aatgtaatca aaatcatctg tcgcttcgaa taattcacta atcatgccat caaatggaat
      721 attgtgtttg ttgagaattt tttgtgtacc ttcatgaggt ggctctccta aattccagct
      781 accagtacct cttgaatgta ctttaatatc atgaatattt ctgtctttaa gtctttgtcg
      841 catgattgct tctgccattg gagaacgaca tatattgcca agacagacaa atgctacatc
      901 taccattttg attcctccaa actatgtagt tatatcccca ttttatagcg actttaaaca
      961 ataagaaagc agattatata aaattctatt aaagtttatt aaattgtgat actttgataa
     1021 cataactatt attagaggtg aacattgtgg ctatgacaaa tgaagagaaa gtattagcta
     1081 ttagagagaa gttaaatatt gttaatcaag gattattaga tcctgaaaaa tataaaaatg
     1141 caaatgaaga agaattaaca gatatatatg attttgttca atcaagagaa agattgtcgc
     1201 caagtgaagt gacagctatt gctgacgctt taggacaatt gcgacacgaa taggagtggg
     1261 aattttgact aattacaaag aaaagttaca acaatacgct gaactattag ttaaggttgg
     1321 tatgaatgtt caaccaaaac agccagtatt tattagatca tcagtagaaa cattagaatt
     1381 aacgcattta attgtagaag aagcatatca ttgtggcgct tcagatgtac gtgttgtata
     1441 tagcgatcca accttaaaac gcttaaaatt tgaaaatgaa tctgtggaac attttgctaa
     1501 tcatgagata aaatcatatg atgttgaagc gagaatggat tacgttaaac gtggcgctgc
     1561 taatttagca cttattagtg aagatcctga tttaatggat ggtatcgata gccaaaaatt
     1621 acaagcattt caacaacaaa acgcacgcgc atttaaagga tatatggaaa gtgtacaaaa
     1681 aaatcaattt ccatgggtcg tagctgcttt tccatctaaa gcttgggcga agagagtgta
     1741 tcctgaacta tctgtagaag aagcatatat taagtttatt gacgaggtat tcgatattgt
     1801 gcgaattgat ggtaacgatc cagttgaaaa ttggcgtcaa cacatcgcta atttaagtgt
     1861 ttacgctcaa aagttacaac aaaaaaatta ccatgcgttg cattatgtat ctgaaggtac
     1921 tgatttaaca gtaggtttag cgaaaaatca tatctgggaa gatgctacaa gttatgttaa
     1981 cggcaaggaa caagctttta ttgctaatat accaactgaa gaagtattta cagcaccaga
     2041 tcgaaataga gtagatggat atgttactaa taaattgcct ttaagttata atggcacaat
     2101 tatcgatcaa tttaaattga tgtttaaaga tggagagatt attgattttt cagctggaaa
     2161 aggtgaagcg gtattgaaag atttaatcaa tactgatgaa ggttcaagaa gattaggtga
     2221 agtagcatta gtacctgatg attcaccgat atcaaatcgt aataccattt tttacaatac
     2281 tttatttgat gaaaatgcag catgtcattt agcgattgga tctgcttacg cctttaatat
     2341 tcaaggtgga acggaaatga ctgttgagga aaagattgca agcggattaa atgattcaaa
     2401 tgtacatgtc gatttcatga ttggtagtag tgatttgact atttatggca tattcgaaga
     2461 tggttcaaaa gaactagtat ttgaaaatgg aaattgggca tcaacatttt aataaatgtt
     2521 attttgaggt gctgagtagg aaatgaaaca tgtatttaaa ggtcaaatac gtgattaaag
     2581 tatagattgg gagataaaat aatgacaaat caggacagac caatgaaatc tatgtcagaa
     2641 tcaaaatgtt ataaaaatag acaagttttc cctcaagata cgaatcacca tcatacaatg
     2701 tttggtggta cattgatggc taatattgat gaaattgcag caatcacagc tatgaaacat
     2761 gctggtgcgc aagtagttac cgcatctaca gactcagtag atttcttaaa gccgattaaa
     2821 acaggggaca tattacaata cgtagcgatg gtttcatacg ctgggactag ttcaatggaa
     2881 gtggtcgttc aaattagaat tgacgatgta tttaataaca agcatgattt agctgcatta
     2941 agttacttaa catttgttgc gttagatgat gaaggcaaac ctaaacatgt acctggtgta
     3001 tatccagaag atgatgttga aaaatggttt tatgatacag caccacaacg tgttgaacga
     3061 agaaaagcac gtcgcattga aagtaaacaa acaattgaat atttagctca agtgcaacat
     3121 attagagact aaaaatataa agacagattg ttaaggatga attgaactgt tatgttcgaa
     3181 tattccacct acaatctgtc tttttgatta tgcacgttta tgcttttagt taaaatttac
     3241 cccttttatt atagactatt agtcctatta aatgcccttt attatttaag tagagtactg
     3301 ccagcaatta attgtttttt attttataat agcattaaaa tatgcggaga ctacttatcg
     3361 ataaagtgct tctccgcata attaagagtt tttaaaattg tataatttgt ggttgtttgt
     3421 gcatatgacg ctcattatct ttaaaatcca tattcggata gtacatattt ttaacaagaa
     3481 catttggtcc taaacaacta aactcggaac aatgacaatt taatgattta gcaagatcag
     3541 aggataacca tttatcaaat acatctgtta atttatcttt ttgtatattc gaaattgtac
     3601 ctgtttcatc tccgaaatca gttacgatta cattacctgt aaatacattg acatttaaac
     3661 gactacggcc atccgggtca tttctagtcg ttacattttt agcatttctt aaacgtgata
     3721 gtaacttttg atcttcatca tcctttaagc atggaaacac aggcaaagta ccaaataaca
     3781 tccaaatatc ttcatctctg aaatccaata tatcatgaat tgtctttttc atttccgcta
     3841 gagttaacac atttaattga cttgcaaagt cagctggata catagggtga atctcgtgtc
     3901 tgctacattt catatcatga acgacttctt gatgtatttt tcgtaaatgt ggtagcgtac
     3961 tttgattgag cattgtttcc gcagatacaa acattccttg ttctgataat gtacgtgcat
     4021 tcgaaatcat ttgttcatat aattttaact tagcttttaa cggtggttgc ttcttcattg
     4081 cgccaaagcc aacatttgcg aattcatcag ttgttcccca gttgtgtgag atatgcataa
     4141 catcgatata ttcagcaata tctaaataac gatcttgagg caatgttagg tttgaattca
     4201 tttgtgtata tatacctcga tgatgtgcat actttaatag aggtttaaca acatttctaa
     4261 tagacttttt agaaaacatt ggttcgccac ctgtaataga catcgttcgc agatttggaa
     4321 tttcatcaag tctacgataa attaagtcca tatctaaagg ctcggggtcg acagtttgta
     4381 aagtataacc aactgcacag tggctacaac gcatattaca aagatttgta gttgtaaatt
     4441 cgatgttact taaagttaat tggccatgtt ctttaacatc gttatatgct tcccatggat
     4501 cattgtaaat gctaacaggt tgcttcaatt tgacgctatt catcgtctta aacttccttc
     4561 tttctatttg gttcatatcg gtatgatacc acttcgtatt atcactcgta acaaattatc
     4621 tcttaatttt agttaattga aaaggggatt tttaaaagtc aaaataattt gaatgtcata
     4681 aatatagcta agattatatc gctttaacgc aatttctgtt aggataataa tgaaaaagaa
     4741 aacaaaataa aaggagttaa ctttttatga atgaacaaca aacaatcgaa cagataaaag
     4801 cgcgtttaaa taagtttatt gaggatatcg atcatgtaaa tcctgatgaa gtacgtgttg
     4861 aagatataga tgaatggatt ggattgttag atcagcttga agaaaaggtt aaattagtat
     4921 ctaagtaaat gccacataag atgtgttagg gttagatatc gcgacagtcg cttaatattt
     4981 tttatatctt gtatgtttaa tcactgattt acttgtctat agtaattata tcaatgatat
     5041 atagaaaggt gatttgcaat gactaaaaaa gtagcaatta ttctagcaaa cgaatttgaa
     5101 gatatagaat attcaagccc taaagaggca ttagagaatg caggctttaa tactgtagtg
     5161 attggagata ctgcaaatag tgaagttgtt ggtaaacacg gtgaaaaagt tactgtcgat
     5221 gtaggcattg cagaagctaa accagaagat tatgatgcat tattaattcc tggaggattt
     5281 tcaccagatc atttacgtgg agatacagaa ggtcgatatg gcacatttgc taaatacttt
     5341 actaaaaatg atgtaccaac atttgccatt tgtcatgggc cacaaatact aatagataca
     5401 gacgatttaa aaggtcgtac gttaacagca gtattaaatg tacgcaaaga tttatcaaat
     5461 gcaggcgcac atgtagttga tgagtcagta gttgtagaca acaatattgt aacaagtcga
     5521 gtaccagacg atttagatga ttttaatcga gaaatcgtta aacaattaca atagaagtat
     5581 tgtggttatc gattgttaaa atttatatat tttcgtttga aatgaaatat ggattttagt
     5641 tttaataaat gaataacaaa taaaaggtcg tct
//
LOCUS       DAKTIP010000167         5652 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.169, whole genome shotgun sequence.
ACCESSION   DAKTIP010000167 DAKTIP010000000
VERSION     DAKTIP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.169"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..812)
                     /locus_tag="O0305_002237"
     CDS             complement(<1..812)
                     /locus_tag="O0305_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCY0275354.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDSRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMRKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYD"
     gene            1106..1531
                     /locus_tag="O0305_002238"
     CDS             1106..1531
                     /locus_tag="O0305_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0275355.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTVDGY
                     HTVPYTISVDGITALHRTYFIFPENKNVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            complement(1895..2599)
                     /gene="budA"
                     /locus_tag="O0305_002239"
     CDS             complement(1895..2599)
                     /gene="budA"
                     /locus_tag="O0305_002239"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCY0275356.1"
                     /translation="MTNVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIQDKDFTK
                     ANIDYKDIADEIREAE"
     gene            complement(2636..4300)
                     /gene="alsS"
                     /locus_tag="O0305_002240"
     CDS             complement(2636..4300)
                     /gene="alsS"
                     /locus_tag="O0305_002240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCY0275357.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            4533..4772
                     /locus_tag="O0305_002241"
     CDS             4533..4772
                     /locus_tag="O0305_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275358.1"
                     /translation="MCVVIMRDVGLIDVEDCYDIFGMWCLVNGGLKLSFYGGLKMLFY
                     VGLEFVVLWRYKKPQQDLDLTVTYTFHTYIYRLVL"
     gene            complement(4754..4936)
                     /locus_tag="O0305_002242"
     CDS             complement(4754..4936)
                     /locus_tag="O0305_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275359.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSIYLMSIFL
                     ICLGTASIIYNYKTNR"
BASE COUNT         1839 a          974 c          800 g         2039 t
ORIGIN      
        1 taatcatact taccttcaac aaattcttta acatgcagtg ttatcccttg ataatatgga
       61 tattttgtta atctaaattt ctgctttaat gcttcatctc tactattcga catcaaatat
      121 gcatgttcaa gatagcttaa atatgcttcg tcattgccgc tatctccaaa acctaatatt
      181 gattttactg gtatattata tttcctcatc agaaattgag tagcatataa ttttccagca
      241 tttgacggtg tgaagtcaat atcatacgca ttttcaggat caccagctaa tggattgctt
      301 cgactaaatc tcgctgtata gttttggtca tttgcgtatc tgacaagtgc ttctaggata
      361 cgactatcat tgttgtgatt acctgttgaa tgaaaataaa aattataatg tgtttcatta
      421 ccttcaaatg ctctttgtgg aataaattcg acattatatt gcgcagaaat atgacgtaat
      481 atatcttcaa tatcttcaaa taaaaatggt tcgctcaaga ctttcttttt atatgtttct
      541 gattcaacat attcgccatt attccaagtg aacattttag aactcaaatc agaaaagata
      601 tgtaatggtt taaacgtcat attaacttga tccattttat ccatgacact ttgaaatgta
      661 cttcctgtca atacagcagt gatcacttca ttattattag ttagtttttc caacaatttt
      721 tccatttctc ttaaatgact taaatcttct tcatttgtat tatgtttaaa atatgtttca
      781 tcaaaatcaa acaataatag acgctttgac atactcattc ccccataata ttttaatgaa
      841 tttatctata acttaaatac actataagtc tcaattaaaa gtataagatc gactatatgc
      901 ttttcatgat tattatatac aactaatcaa tccctataaa ctaaaaacat tgcttaccac
      961 ttttcaaaat gcacgagtca tatccctcgt taaaaactat tactaaaaat taaaatataa
     1021 aaaatgcaaa aagtatgttt taaactttat gaaacgtgtt atacaaatag tgtaatatca
     1081 tacaacccga aaaggagatt gagatatgaa actaaaatca tttgttactg ccactttagc
     1141 attgggatta ttatcaacgg tcggagctgc attaccgagt cacgaagcat ctgcagatag
     1201 taataacggc tataaagaaa tgactgtgga tggttatcac actgttcctt acacaatttc
     1261 agtagatggt attactgcat tacatcgaac ttactttatc ttcccagaaa ataaaaatgt
     1321 tctttatcaa gaaattgaca gtaaagtaaa aaatgaatta gcttctcaac gtggtgttac
     1381 aacagaaaaa attaataatg cccaaacagc aacttatacg cttactttga atgatggtaa
     1441 taaaaaagta gtgaatctaa agaaaaatga cgacgctaaa aattcaattg atccaagtac
     1501 aatcaaacag atacaaattg tagttaaata ataatttgaa aatagtttta tagggcaagc
     1561 gctgaaaaat tggcgtttgt ctttttagtt attgaacttg taaattattg ttgtttgctt
     1621 ctcgtagtaa cagattggcc tatacaatag taaattttat atgagtgtgt accacaattt
     1681 ataatctaaa cataacagca aatctcaaat atctggaacc taataaatcc cctcactact
     1741 gctaatagtg aagggattta ttttattact tagacgtaat tataaatata tttgttctat
     1801 tgtagtcatg gttaatcata tttacaaact atcatttcta aatcgcgcat tatgaagtca
     1861 attttcataa accgtcatta tatttctgga ttcattattc agcttctcta atttcgtctg
     1921 caatatcttt atagtcaata tttgctttag tgaaatcttt atcttgaatt ggaaaatgct
     1981 gttcaaatgt ttctatattt tggatttcta ctttaacatc ttctacttca aaatctaaga
     2041 catgtccacc aaagttacga tcatcattcg caaagtgtac atgaaatcct gctgatccga
     2101 taccatggaa taattctggt gtaaagaaac caacgactga acctttgaca tacgtttcag
     2161 tttgttcagg ttgtcttcta gctgaatcga ttaaacgtgt ataaggtggt tcttgagccg
     2221 gcatcatacg tacatgcatt tttttaaata agcctgaaat ttttactgct gaaaataaat
     2281 tttcactcaa catcttttcc ttaatttctg caaaaactgc ttctgaagat ttatctttcg
     2341 tttcatagct tgtatctgct acaaatttag ttacagttgc atatggtgtt aactcgtcac
     2401 cttttaattc tacaaattct ttatgttcat ttgcatggta agcttttcca tctaaaaaga
     2461 ttacctcacc gtttgaacct gttagtgtag cgatacctaa gtcaccatgt tgcaataatt
     2521 catttattga tgcagttcct tttaataatc ctgccattaa tgtgcctaat gtaccatgtt
     2581 ggtataaaac attagtcata aaatctccca ctcctcttta acatttatct ttattttagt
     2641 taaatacgtc aggtaacata tttgttgaaa gtttgatatt atctttgtaa tttacaggta
     2701 tatcaattaa tactggacca tctgtttcat agccctcttt aattgccgct tctaattctt
     2761 cttgattagt aactcgtaaa cctttcgcgc caaatgattc tgcatatttt acaaaatcta
     2821 caggaccgaa gtctacacct gacgaacgtt tatatttcat ttcttcttgg aattcaacca
     2881 tgttatattt tccatcattc caaataagct ggatgatatt taaattttta cgtacggccg
     2941 tttctaaatc ttgtgatgaa aataaaaagc caccatcgcc agcaacggac acaacttgcg
     3001 tattagggcg cacaagtgca gctgaaattg cccacggtaa tgctacacca agcgtttgca
     3061 taccattgct aaataataaa tgtcttggat tgtaacttct gaaattacgt gccatccaaa
     3121 tatagtgact tccaacatca actgttacag tagtatcatc agttaatacc ttttgcatag
     3181 attcaataat ttccactgga tgtagaattc cttcttcatg cgtcgcttta ataccagttt
     3241 cttcatcaat atgtgttctt aattgttcta attcgtctaa atgttgttga tttataaatg
     3301 gctcatccac tttttcagaa atcatttcaa tcgttttagc aatattacca atcaactctt
     3361 ttttcggttg catataatta gtaatttcag cttgaacttc gtcaatattg ataatttgtg
     3421 tttctaattc tttattccag ttactagctt cgtattcaat tggatcataa ccgattgtaa
     3481 caactaaatc acttttacgt aataattcat caccaacttg attgcggaat aagcccacac
     3541 gaccgaagaa atgattttct aattcacgac taattacacc tgcaccttgg aatgtttcta
     3601 caactggtaa attcgtgcgc tcaactaatt tgcgaatggc atttgtttct tctgcacttg
     3661 aacttctcat accagctaat aaaacaggaa atgatgcatt tttaatcgct tcaatgacat
     3721 cattaatatc ttgttcactc ggtactccta aatttggttt ttggcaaagt gatatagctt
     3781 tagattcaac tggtgaagaa ataacgtctt gcggaatact aataaaactt gcgccatttt
     3841 ttcctgaagt agcaattcga attgcatttg tcataacttc tgataatgat tcaggatctt
     3901 gtacttcttc actatatttt gatgaatatt ttaatagcgc agcattatca atactttgat
     3961 gcgttaatcg taataaatca ttacgtttca cttggccacc taacgctaat acaggatccc
     4021 cttcagatgt tgcggttaat aatccagtag ttaaattact tacaccagga ccacttgtaa
     4081 caagtactac acccggtttg cctgttaatc ttccaatacc ttgcgccatc attgcggcgt
     4141 tttgttcatg acgagtgaca ataagttcag gaccatcatc aattaaagca ttaaatagat
     4201 agtcaatctt tgcacccgga ataccaaaaa catattctac cccattattt ttcaaagtat
     4261 caataaccat atcggctgca gtgtactttt tatcagtcat ttatattcat ttcccttcaa
     4321 atgtgatgta tagatgtgta ttacatgtaa ttactttgtg atttgtaatc taatgatagt
     4381 tacgcaaaat taatgatgtt tatcaaaatt ttacatgtac gattagattt gtaatacata
     4441 aaaattgcct tcagtacatt atataacctt tgttagatga ataaaaatcg ttatttgtaa
     4501 tactaagtat tccaaaaatg catgaataga ttttgtgcgt tgtgattatg agggatgttg
     4561 gattgattga tgtagaggat tgttacgata tttttgggat gtggtgttta gtcaatggcg
     4621 ggctgaaatt gtcgttttat ggtgggctga aaatgctgtt ttatgtcggg cttgaatttg
     4681 tcgttttatg gagatataaa aagccacagc aagacttaga tctaactgtg acttatacat
     4741 ttcatactta tatttatcga ttggttttat agttataaat gatagaagca gttcctaaac
     4801 aaattaagaa gatactcatt aaatatatag aaaagttatc tgttttaata taaaacctaa
     4861 aaagtagaaa caccagcatc gcaacaataa aataaacagg aataaaagct ttcttccaaa
     4921 atatagaatc tttcaattaa gtcactcctt attttgataa cactcttaaa tcatggttct
     4981 tgagttatca tctggttatc atccgatagc tctttccctt gaatatctat tattagaaca
     5041 ctatcgtacg gtctccgcaa gctaaatgac aatttaataa tgtacacttt tgattgtgta
     5101 agcatgtaca aattatacgt gtaaatcaat aattcatagt tgaattaatt ctatacctga
     5161 gacattaatt catttatact ctattccata ttccccacac aaacgcaaaa aaacacctcg
     5221 acataacatc gaggtgtttt gtatatatat cgtccgacaa ttaacgtttt gagaattgag
     5281 gtgaacgacg agctgcttta agaatttgtt tttttacgtt cagaaaattt tagaatttcg
     5341 catgattata tagactgtcg tacaccttga tattaaagga tttcttcaat tttgaatttc
     5401 attgattttc atcaaattga agcaaagggt attaacgggg tattaatttt ttaaaaaaat
     5461 ctgctggtcg gtggattcaa taattgatct tatttactgt atttgacttc tatacttttg
     5521 aaaaaatttt ctatatcaac tttatccata tttaaaatat ctttattgaa cacacaatgc
     5581 aaatatggtt ttgcagaaaa aaaagtattg ccttcactta tcagttgata attaacatcc
     5641 ttaaaattta aa
//
LOCUS       DAKTIP010000168         5651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAKTIP010000168 DAKTIP010000000
VERSION     DAKTIP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.170"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            25..933
                     /locus_tag="O0305_002243"
     CDS             25..933
                     /locus_tag="O0305_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCY0275360.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            1332..2273
                     /locus_tag="O0305_002244"
     CDS             1332..2273
                     /locus_tag="O0305_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCY0275361.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            2394..2948
                     /locus_tag="O0305_002245"
     CDS             2394..2948
                     /locus_tag="O0305_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCY0275362.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGIK"
     gene            3020..3790
                     /locus_tag="O0305_002246"
     CDS             3020..3790
                     /locus_tag="O0305_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275363.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(3962..4759)
                     /gene="fdhD"
                     /locus_tag="O0305_002247"
     CDS             complement(3962..4759)
                     /gene="fdhD"
                     /locus_tag="O0305_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCY0275364.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPMQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            5049..>5651
                     /gene="modA"
                     /locus_tag="O0305_002248"
     CDS             5049..>5651
                     /gene="modA"
                     /locus_tag="O0305_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCY0275365.1"
                     /translation="MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQV
                     AAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVD
                     ALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQY
                     LDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTD"
BASE COUNT         2065 a          748 c         1046 g         1792 t
ORIGIN      
        1 aatcattttc aataggagga aattatgaaa aaactattat taccattaat aattatgtta
       61 ttagtgttag ctgcgtgtgg gaaccaaggt gaaaaaaata acaaagctga aactaaatct
      121 tataaaatgg acgatggcaa aacggtagat attccgaaag accctaaacg cattgcagta
      181 gttgcgccaa catatgctgg tggacttaaa aaattaggtg caaacattgt agctgtaaat
      241 caacaagtcg atcaaagcaa agtattaaaa gataaattta aaggtgttac aaaaattggt
      301 gatggcgatg tagaaaaagt tgctaaagaa aagccagatt taattattgt atactctact
      361 gacaaagata ttaaaaaata tcaaaaagta gcaccaacag tagttgttga ctataataag
      421 cataaatatt tagaacaaca agaaatgtta gggaaaattg ttggtaaaga agataaagta
      481 aaagcttgga agaaagattg ggaagaaaca actgctaaag acggtaaaga aattaaaaaa
      541 gcaattggac aagatgcaac agtgtcattg tttgatgaat ttgataaaaa attatacact
      601 tacggcgata actggggtcg tggtggagaa gtattatatc aagcatttgg tttgaaaatg
      661 caaccagaac aacaaaagtt aactgcaaaa gcaggttggg ctgaagtgaa acaagaagaa
      721 attgaaaaat atgctggtga ttacattgtg agtacaagtg aaggtaaacc tacaccagga
      781 tacgaatcaa caaacatgtg gaagaatttg aaagctacta aagaaggaca tattgttaaa
      841 gttgatgctg gtacatactg gtacaacgat ccttatacat tagatttcat gcgtaaagat
      901 ttaaaagaaa aattaattaa agctgcaaaa taattcagct atataagtta gtgaaatgag
      961 agtctgaaac atatcaatct tttgatattg tattaggctc ttatttttat agctagaaag
     1021 ttagatattt gtattttttt aaataataag tgccgttgtt atcgttcaat ttaattaatg
     1081 atagattagt attattatag ctaaagtagt atacctgaga aaatagctca atgtatctct
     1141 ttattaataa gttatatcat aattatttta gtgcatactt tatggaaggg atatcaggga
     1201 atggctttca attaaagaag aggtttaaaa ggattacaac agaatgttat gattttgtag
     1261 aaagatatat aacaacgttt tataaaaaca taatattgtt aatggaaaat gaaatgtaag
     1321 ggggatttcg agtgactaag aaagtttatt ttaaccacga tggtggtgta gatgatttag
     1381 tatctctatt tttattatta caaatggaaa acgttcaatt gataggggtc agtacaattg
     1441 gtgctgattg ttatttagag ccatctttga gcgcatcagt aaaaattatt aatcgttttt
     1501 caaatgaaga tattcaagtt gcgccatcat atgaacgagg aaaaaatcca tttcctaaag
     1561 aatggcgtat gcatgccttt tttatggacg cattgccaat tttaaatgag ccagtcaaac
     1621 atgttgcttc aaatgtgagc gacaaagaag cctttgaaga cattattcaa actttaaaga
     1681 gacaatcaga aaaagtaaca ttattattta caggcccgct tacagattta gcaaaagcac
     1741 tacaaaaaga ttcatctatc gttcagtata tagaaaaatt agtttggatg ggtggcacct
     1801 ttttaccaaa aggaaatgtt gaagaacctg agcatgatgg ttctgcagaa tggaatgcat
     1861 attgggatcc agaagcggtt aaaattgttt ttgatagcga tatagagatt gatatggttg
     1921 ctttagaaag tacgaatcaa gtaccgctaa cgttagatgt tagacaaaga tgggcgaatg
     1981 aacgccaata tacgggcatt gattttttag gagtaagtta tgcagcggta ccaccattaa
     2041 cacactttat aacaaattct acttactttt tatgggatgt tttaacgact gcttatattg
     2101 gtaacaagga cttggttcat tcaattgaga aaaaagtcga tgtaataagt tatggaccaa
     2161 gtcaaggtaa gacatttgag tgtaaagatg ggcgcaaaat taatgtcata aatcatgtag
     2221 ataacaacgc attttttgat tatataactg cacttgctaa aaaagtaaat taacagctgt
     2281 gtagaataat taaggtttta atttatatag aacaacttat tgtaaacttt tcatttctta
     2341 aagtttacaa tggtgctata ataatggtca tgaaatacga aaggaagtaa aaaatgacaa
     2401 caaaacagtt agtatataca gctttaatga cagcgattat cgctatttta ggattggtac
     2461 cggtaattcc actaccattt tcttcagtac caattgtact tcaaaacatt ggtattttct
     2521 tagcaggtgt gattttagga cgtaaatatg gcacattaag tgttatcgtc tttttattat
     2581 tagtagttgc tggcttgcca ttgttatcag gtggtcgcgg tggcatcggt gtattcgcag
     2641 gtccttcagc agggttttta ctattatatc cagttgtagc attcatgatt ggggcgattc
     2701 gagatagatt catcaatgaa attaatttct ggattttatt cgttggtatt ttagtttttg
     2761 gtgttatagc attagatgtt attggtacat tgattatggg catgattatt aacataccat
     2821 ttacgaaagc tatttcaatt tcattagctt atttgcctgg tgatatatta aaagcaattg
     2881 tagcaagttt gattggtaca gctttactta atcactcgca gtttcgtcaa attatgggaa
     2941 taaaataatc atatttaaga tagtaaagta attgaataag ttgctttgaa atttataaaa
     3001 gtgaaaggag taggtgtcaa tggctagtat aagtatgtca gatatatatt gtaacggcac
     3061 tatatttgaa aatgacgacg agcagttgat ttatttaacg ccttcttttc cacaacgata
     3121 cacaagtaac acatggatat ataaaaagac gcctacccaa gagcgatggc tgaaagactt
     3181 agaacgtcaa catcaattac atacaaatca aggttcaaat cattatgcgt ttagtttccc
     3241 ggaaaatgaa caacttgata atcattggat ggctatgttt aaagatatga attttgaact
     3301 aggtattatg gaattgtatg ccatagaaag tgatgcgctt gccaatttgc cgcgtaactc
     3361 tgacgttgaa attgccatcg ttgacgagtc gcatatagat gcctatttaa aagttgcata
     3421 tcagtttagt ttgccatttg gaaaagacta tgcagatgca catgaagaaa tggtaaggga
     3481 acattatcaa aaagatgtga ttaaacgctt agtagcttat ttaaataatg aacctattgg
     3541 cgttgtagat gtcattgaaa gtgaaaatta cattgaatta gatggatttg gtgtattaga
     3601 acaatttcgg caccaaggaa ttggatctac aattcaatcg ttgataggtg aatacgccat
     3661 atcaaaaaat cacaaaccaa tcatattagt tgcagatggt gaagatacag caaaagatat
     3721 gtatgcaaag caaggttatg tctatcaatc gttttgttat caaatattaa aagaagatat
     3781 tggaaattaa cgattgatga taggtttgta ttagaataaa aaagcagtaa gctggtttcg
     3841 agtagaaatc aacttactgc tttttaaatt gttttgagct acttatactt ataaaaatag
     3901 tgcgtttaaa ttgttgattc atgtagaata tcgttcatta tgacacacta taatgaatat
     3961 gttattgttc agaatcaatg atacgttctg gatgactgta tatattaaag ccaccatttc
     4021 gaataaatcc aactgccgta atatttaggt cattagctaa ggttacagca agcgttgtcg
     4081 gagctgattt agataaaatg acgccaacac caatttttgc ggctttaatt aaaatttctg
     4141 atgaaatacg tccactaaaa attaatactt tatctcggac agtaatatgt cgctgaatac
     4201 aaaatccata taatttatct agagcgttat gtctaccaat gtcttgtcga tgtacaaaaa
     4261 atgtcaaacc atcgcttata gcagcattat gtaagccacc tgtttcttgg taaatatgac
     4321 ttgcactttg taatcgagtc atcatgttaa taatttgcat tggagttaaa gtgattttag
     4381 acatagatgt tttagcgata gcagcatcat tttgaaaata aaactcacga ctctttccgc
     4441 aacaagatgc aatcattcgt tttgtggaat attgaaagcg atcgcctaaa tctttattaa
     4501 gttcaacatg ggcaaaacct ttactatcat caatcagtac agattttaat tcatctcgct
     4561 ttaaaatggc accttccgaa gccagaaatc caatgactaa ctcctcaagg tttgttggac
     4621 tgcatataac agtcgcaaat tcttcaccat tcaccataat tgtaagtgga aattctgtca
     4681 catattgatc tgttgtattg aataattttc catcttcata tctaacaatt ggttgaccta
     4741 aagatacatc tttgttcatt atctaacccc tttaattagc ttaaacttta ttttaaagca
     4801 atttgcttaa aattttaaca tatttgctta agtttgaaat ttgattgata aaaattaata
     4861 gcgagcaatc tgtttgattt aaattgaatt cgagaatata catactaggg catcaattaa
     4921 taaatatcaa tcttatgcaa atttgacaat tgtttgaatc aatatataaa caggcaacgg
     4981 ttcttttcaa atataatagt aagtgtataa tgaaaatgta aatattatta aaaatggggg
     5041 ttcactcaat gaaattgaaa cgtttatttg ctgttgtgat tgcaatgctt ttagtattag
     5101 ctggttgctc taattctaac gataataatg aaagtaaaaa agatgacgca gacaatggta
     5161 agaaacaaga gattcaagtt gcagcggcag caagtttaac agatgtaacc aagaaattag
     5221 cttcagaatt taaaaaagag cataaaaatg ctgatattaa atttaactat ggtggatcag
     5281 gggcattaag aaaacaaatt gaatcaggcg cacctgttga cgtatttatg tctgcaaata
     5341 ctaaagatgt agatgcatta aaagacaaga ataaagcgca tgatacatat aaatatgcga
     5401 aaaatagtct agtattaatt ggtgataaag attcaaatta cacttcagta aaagacttaa
     5461 aagacaatga taaattagca ttaggtgaag tgaaaactgt accagcagga aaatatgcga
     5521 aacagtattt agataacaat aacttattta aagaagtcga aagtaaaatc gtttatgcta
     5581 aagatgtaaa acaagtatta aattatgttg aaaagggtaa tgcgaaacaa ggttttgtgt
     5641 ataaaactga c
//
LOCUS       DAKTIP010000169         5603 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAKTIP010000169 DAKTIP010000000
VERSION     DAKTIP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.171"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            186..1643
                     /locus_tag="O0305_002249"
     CDS             186..1643
                     /locus_tag="O0305_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0275366.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1636..2358
                     /locus_tag="O0305_002250"
     CDS             1636..2358
                     /locus_tag="O0305_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCY0275367.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2509..3636
                     /gene="queG"
                     /locus_tag="O0305_002251"
     CDS             2509..3636
                     /gene="queG"
                     /locus_tag="O0305_002251"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCY0275368.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3641..4111
                     /gene="trmL"
                     /locus_tag="O0305_002252"
     CDS             3641..4111
                     /gene="trmL"
                     /locus_tag="O0305_002252"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCY0275369.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4270..4869
                     /locus_tag="O0305_002253"
     CDS             4270..4869
                     /locus_tag="O0305_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCY0275370.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4894..5046
                     /locus_tag="O0305_002254"
     CDS             4894..5046
                     /locus_tag="O0305_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275371.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
BASE COUNT         2019 a          757 c         1074 g         1753 t
ORIGIN      
        1 agtagcgctt ttttgattga ttttaaaatg tgagatttgt tcacgtataa tagcggattt
       61 tttatgtaca gtcttttaaa aagaagcttt tctttatatt gataaaaata catttataaa
      121 acactattgc atcaaaatgt attaatatgt ataattatga atattaatta gggagggctg
      181 tataaatgaa gtgtttaatt aggtttatat tagtattagg gttgttaatt agcagtgcta
      241 tggtgtatat caatcccaca gcacatgctg aacaagatca aacatgggag aagattaaag
      301 aacgcggtga acttagagtg ggtctttctg cagattatgc accaatggaa tttgagcata
      361 cagttaatgg taagactgag tatgcaggtg tagatattga tttagctaaa aaaattgcga
      421 aagataataa tttaaaatta aaaatcgtca atatgtcatt tgatagtttg ttaggagctc
      481 ttaaaactgg aaaaattgat attattattt ccggaatgac ttcaacgcct gaacgtaaga
      541 agcaagttga tttttcagat tcatatatga tgactaaaaa tatcatgctt gtaaagaaag
      601 ataaagttaa tgaatataaa gatatcaaag actttaataa taaaaaagta ggggcacaaa
      661 agggaactga acaagaaaaa atcgctcaaa ccgaaattga aaatgcatct attacttcat
      721 taagccgttt accagacgtt atattagcat tgaaaagtgg aaaggttgaa ggtgctgtag
      781 ttgaaaaacc tgtagcagaa gcatatttaa aacaaaatcc taaattagga atttccaatg
      841 tgaaatttaa tgaagaagaa aaagatacag tgatagcagt gccaaaagat tcaccaaaat
      901 tattgtcaca aattaataaa acgattaagg aggttaaaga taaaggatta atcgataaat
      961 atatgactaa tgctgcaaat gcgatgaatg atgacagtgg ttttatttct aagtatggta
     1021 gttttttctt gaaaggaatt aagattacaa tattaatttc acttatcggt gttgcattag
     1081 gttctatttt aggtgcattc gttgcgttaa tgaaattaag taaaataaaa attatttcat
     1141 ggattgcttc tatctatatc gaaatattaa gaggaacacc aatgttagtt caagtattta
     1201 tcgtattctt tggtataact gctgcattag gactagacat ttcagctctt gtttgtggaa
     1261 caattgcatt agttattaat tcctcagctt acattgctga aattattcgt gcaggtataa
     1321 atgctgttga taaaggccaa atggaagccg cacgtagtct aggtttgaat tatagacaaa
     1381 cgatgaaaag tgtaattatg ccacaagcaa ttaaaaatat tttaccagct ttaggtaatg
     1441 aatttgtcac tttaattaaa gaatcatcta ttgtgtcaac aattggagtt ggcgaaatta
     1501 tgtttaatgc acaagtggtt caaggtattt catttgaccc attcacacca ttaatagtgg
     1561 cagcagcatt atactttgtc ttaacatttg tacttacacg cattatgaac atgattgaag
     1621 ggagattgaa tgccagtgat taaaataaac aatcttaata aagtttttgg agataatgaa
     1681 gttttaaaag atatcaatct tgaaatcaat caaggggaag tagtagcaat aataggtcca
     1741 tctggtagtg gtaaaagtac attgttaaga tgtatgaatt tattagaagt acctactaaa
     1801 ggtcaagtga tttttgaagg caatgactta acggaaaaag ggacacaagt agataaacta
     1861 cgtcaaaaaa tgggtatggt atttcaaaac ttcaacctat ttccacataa aaaagttgtc
     1921 gataatatta ttttagctcc taaattatta aagaaagata ataacgatga attacataag
     1981 gaagcattgt cgttattaga taaagtggga ttaaaagaaa aagcagatgt atatccgaat
     2041 caattatcag gtggtcaaaa acaaagggta gcaattgcaa gagctttagc aatgcatcca
     2101 gatgttattt tattcgatga accaacttca gcattagatc ctgaggtagt tggtgatgta
     2161 ttaaaagtaa tgaaagacct agccaaagaa ggtatgacca tggtggttgt gacacatgaa
     2221 atgggatttg ccaaagatgt aagtgacaaa gtcatattta tggcagatgg cgttgtcgta
     2281 gagtcaggca caccagtcga aatatttgaa caaccgcaac atgaaagaac acaaaatttc
     2341 ttagcaagag tattataaca acctaacgaa gcttgaatat atgatacgca ccacaaagtt
     2401 atatcatata ttcaagcttt ttattattaa aattatgtga aatgaaacct caatatgaat
     2461 aatggttgtg ttaaaataac atagtattat tttatggggg gcatacgatt ggatacaaag
     2521 caattaaagc aagatatcat tgactatgca tatacaattg gtatcgacag tattggattt
     2581 actactgccg atccctttga tgaattgaag caaaagctag aagcatatca tgcaaatggt
     2641 tatgcctcag gatttgaaga atctgatatt gctttacgaa cggagcctaa attatcctta
     2701 ccaacagcaa ggtcaatcat agcaattgca gttggttatc ctaacaaact gaaaggtgca
     2761 cctaagagtg ttagaggtga tcgcagaggc ttatttgcta gagcatcgtg gggtcaagat
     2821 tatcatacaa ttatgcgtaa acgattagac atgttagctg catttattga atctaaagtt
     2881 ccagatgttg aaatcaaatc tatggtagat acgggtgtat tatcagatag ggcagtagca
     2941 gaacgtgcag gtttaggatt tgttggtcgt aatggctttg tcatcaatcc taaactagga
     3001 acatggacat acctcggtga aatgttagtc agtatacctt ttgaacctga tgatccatta
     3061 ttagatagct gtggtgattg tacaatttgt gttgatcgtt gtccaacaag tgcattagtt
     3121 ggaaatggcc aattaaatag tcagaaatgc attagttttt taacgcaaac aaaaggctat
     3181 atgcctgacc aatatcgtta taaaattggg aatagattat atggttgcga tacgtgtcaa
     3241 caagtttgtc cgaaaaatag aggtattaat accgaacaag atgacatcat tttggaacca
     3301 gaaattttaa agccaagatt agtaccttta ctacgtatgt ctaataaaga attcaaacaa
     3361 acatatggtc accttgcagg tgcttggcgt ggtaaaaagc cgatacaacg aaatgctatt
     3421 ttagcattgg cgcattttaa tgaagtggat gcaattccag aattgaaaaa agttgcaaca
     3481 acagatgaaa gaccgttgat tcgagcaact gcttattggg ccattggtca aattcttggg
     3541 gaagaagcaa gagattttat caatgctaat tatgatcaag aggatgcaga agttcagaat
     3601 gaaatgataa aaggattaga cacaaggaga gaatagtaat atgacaaatc atatcgtttt
     3661 atatcaacca gaaatcccag ctaatactgg taacattgca cgtacgtgtg caggtacaaa
     3721 cacacattta catttaatta aacctcttgg atttagaact gatgataaaa tgttaaaaag
     3781 agccggttta gattattggg aatttgtgaa tattacgtat catgatagta ttgaagcatt
     3841 ttttgaagtc acaaatggcg agtattactt attaacaaaa tttggtaaaa agacttatag
     3901 tgattttgat ttttcaaatc atgacaaaga tttttacttc atttttggaa aagaaacgac
     3961 aggattacca gactgggtga aagagaaata tcaagacaca gcgttaagaa ttcctatgag
     4021 tgaacatatt cgttcattaa atttatcaaa tactgctgcg ttgttaattt atgaagcgtt
     4081 acgtcaacaa gattttccgg gattgaatta aaatttactc attttcatta taaaaatgat
     4141 gcttaagtgc tatatttaga gtataagcat gttaaagtta aatggtttga tatattgaaa
     4201 gttagggtat aataattatt aatatcgccg tttttatgta aaatgcaaac taagaggagt
     4261 gtagaaatca tggcaatgaa ctttaaagtc tttgacaata gtcaacttgt agcagaatat
     4321 gctgctgata ttattagaaa gcaatttaac aataatccta ctacaattgc aggttttcat
     4381 ttagatacag atcaagcgcc agttctagat gaattaaaga aaaatgttga aaaacatgct
     4441 gttgatttta gccaaataaa tattttagat tatgacgata aaaaatcata tttcgaagcg
     4501 ttaggtgtac cagcaggtca agtttatcca attgcttatg aaaaagatgc aatcgaatta
     4561 atcgctgata agattaaaac taaagaaaat aaagggaaat taacattaca agttgtttct
     4621 atcgatgagc aaggtaagtt aaatgttagt attcgtcaag gactaatgga agcaagagaa
     4681 attttcttag tagtgacagg tgctaataaa cgagatgtag ttgaaaaatt atatcaagaa
     4741 aatggtaaaa caagcttcga accagccgat ttaaaagcac atagaatggt aaatgttatt
     4801 cttgataaag aagcggctgc aggtttacct gaagatgtta aagcttactt tacgtcacgc
     4861 tttgcttaat taaaaatctg tgaggtattg attatgtcta aagacaaaga tccaaaatta
     4921 aattatcatg aagaagaaaa cagtatggta acggattttg aagatttaaa agaattaggt
     4981 aaagaaatgg aacaaatctc tgatcaaaat gatcaagaaa agaattctga agaagacagt
     5041 cagtaaagta ttataattgt gtaatattca gttatttatc atgtaaataa tgtatagtat
     5101 agaacatata tcaaatagga ttgattatct tgaatatgaa gttaggtagc attttgttac
     5161 ctagcttttt tattatattc atagaagctg actttctgct agtttcagtg ttggggctgt
     5221 ttccccaact tacattgttt gtagaaattg aggatccaat ttttctatgt tgtggccccg
     5281 ccaacttgca tgttatttta agctgacttt ccaccaactt ctgagtttgt ggcccgtttc
     5341 cctaatttgc aatgtctgta gaaattagaa atccgatctc actattttgg ggcctcgcca
     5401 acttgcatgt tattttaagc tgactttcca ccagtttctg agtttgtggc ccgtttcccc
     5461 aatttgcaat gtctgtagaa attgggaatc caattccact atgttggagc tccaccaact
     5521 tgcatgaaat agaagctgac attccgaagc ctctatgttg tggactgcag tcaactactg
     5581 ccaatataac tttgtaaagc ata
//
LOCUS       DAKTIP010000170         5533 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAKTIP010000170 DAKTIP010000000
VERSION     DAKTIP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.172"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1137
                     /gene="mnhD1"
                     /locus_tag="O0305_002255"
     CDS             <1..1137
                     /gene="mnhD1"
                     /locus_tag="O0305_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCY0275372.1"
                     /translation="GIIGAFTTGDIFNLFVYFEVFLMSSYFLLVIGSTKIQLQETIKY
                     VLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIFVFAT
                     KAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSHYVIL
                     FLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYTLHDM
                     LVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSGFYGK
                     FFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVKYLTT
                     IAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            1139..1618
                     /gene="mnhE1"
                     /locus_tag="O0305_002256"
     CDS             1139..1618
                     /gene="mnhE1"
                     /locus_tag="O0305_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCY0275373.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            1618..1911
                     /gene="mnhF1"
                     /locus_tag="O0305_002257"
     CDS             1618..1911
                     /gene="mnhF1"
                     /locus_tag="O0305_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCY0275374.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            1889..2245
                     /gene="mnhG1"
                     /locus_tag="O0305_002258"
     CDS             1889..2245
                     /gene="mnhG1"
                     /locus_tag="O0305_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCY0275375.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            2494..3648
                     /locus_tag="O0305_002259"
     CDS             2494..3648
                     /locus_tag="O0305_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCY0275376.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(3704..4078)
                     /locus_tag="O0305_002260"
     CDS             complement(3704..4078)
                     /locus_tag="O0305_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCY0275377.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(4097..5413)
                     /locus_tag="O0305_002261"
     CDS             complement(4097..5413)
                     /locus_tag="O0305_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCY0275378.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
BASE COUNT         1842 a          954 c          864 g         1873 t
ORIGIN      
        1 ggtatcattg gtgcattcac aactggtgac attttcaacc tattcgtata tttcgaagta
       61 tttttaatgt catcttattt cttattagta attggttcta caaaaattca actacaagaa
      121 acaataaaat atgttcttgt taacgttgta tcgtcatcct tcttcgtaat gggtgtagcg
      181 attttatatt cagtagtagg aacattgaat ctagcagata taagcaataa attagctaac
      241 ctttcagcgc atgacagtgg cttagtcaat atcgttttca tactatttat ctttgttttt
      301 gctacaaaag ctggcgtatt cccaatgttt gtttggttac ctagtgcata ctatgcacca
      361 ccaattccaa tcattgcatt ctttggtgct ttgttaacaa aagttggtgt ttatgcaatc
      421 gctagaacat tgagcctatt ctttagtgat aatgtaagct tttcacatta tgtcatttta
      481 ttcttagcac tattaacgat catctttggt tgtgtcggtg ctgttgcata tgccaacatt
      541 aaaaaaatca tcctatacaa tgtcatgatt gctgtaggtg tgatcttagt cggcgttgca
      601 atgatgacag agtcaggtat gattggggct atttactata cattgcacga catgttagta
      661 aaacttgcat tattcttgct tatcggaatt atgattaaaa ttacaggcac tgctgattta
      721 agacaatttg gtggcttaat taaacgatat cctgtacttg gttggtcatt ctttatcgca
      781 gcattaagtt tagctggcat tcccccactc agtggctttt atggaaaatt ttttattgtc
      841 caatcaacat ttgaaagagg attttattta agtggtgtca tcgtcttatt atcaagttta
      901 gtcgtattat attctgtcat tcgcatattc ctacaaggct ttttcggtca accaaaagga
      961 tatgatctta ataacaaagt cgacgtcaaa tatttaacta cgattgcaat cgttgcagtt
     1021 gtgataactg ttttatatgg attgtcagca gattacttat atccaatggt aaaagctggt
     1081 gcagaaacat tctacaatcc atcaacctac gttaaagctg tattaggagg taaataacat
     1141 ggctgtacaa ttagtattaa actttattat cgcagtgttt tggttgtttg tgacaaatag
     1201 ttacacaaca aataactttg tgttaggttt catctttggc ctagttcttg tttacctctt
     1261 acacagagtg ttaccaggta gattttatgt catcacgcta tatcgtatta ttaaattagt
     1321 tattattttc ttaatcgaac tgattaaagc taattttgat gttttgaaaa ttattattaa
     1381 accttcgatt aaaaatgaac caggattctt tgtttatcac acagatttaa aaaaagattg
     1441 gcaaattgtc ttgctgtcta atttaattac attaacacct ggtactgtag tgcttggtgt
     1501 aagtgacgat cgtacaaaaa tttatattca tgccatagat ttttcaacta aagagcaaga
     1561 agtagaaagt attaaaacat cattagagaa aattgtaaga gaggtgggcg aaatataatg
     1621 aatcataatg ttattatcgt tattgcatta atcatagttg tcatttctat gttagctatg
     1681 ctcattcgcg ttgtgctagg cccatcactt gccgatcgtg ttgtcgcatt agatgcgatt
     1741 ggtcttcaat taatggcagt tatagcatta ttcagtattt tattaaatat taaatacatg
     1801 attgtcgtta ttatgatgat tggtatatta gcttttttag gtactgcagt attctctaaa
     1861 tttatggaca aaggtaaggt gattgaacat gatcaaaatc atactgatta gtcttgcact
     1921 tatctttgtt atcatcggtg ctttaattag cgccctagca gctataggat tattgagact
     1981 tgaagatgta tattcacgtg cacatgctgc cggaaaagca tcaacattag gtgcaatgtc
     2041 attactattt ggtacgtttt tatattttat tgctactcaa ggttttgtaa atatgcaatt
     2101 aatcgttgcg attatctttg tattaattac aggtcctctt tcaagtcata tgattatgaa
     2161 agcagcatat aatattaaaa cgccttatac taaaaagact aaagtcgatg aaatttcgga
     2221 agacttaaaa gacacaaaat tgtagattat ataacaaaat catgaatata aatcaacaac
     2281 aaacagcagt aagatgattc caaattagga atgattttac tgctgttttc ttttgacatt
     2341 gttacctctt tttcaatgat tttttctttg actacagatt cgccctatct acatatatct
     2401 ctttaattta attgcctttc atgtcgttat gtattatgat aataataatt ataaatcgta
     2461 acgattacgt tttaaaaaga gagaggtttt attatgcatt ggacaattat cggcggtggc
     2521 atacagggaa ctgcaatcgc acaaaaacta ttatcaagcg gattaacaac agaccgatta
     2581 acaatcattg acccacacga aactttttgc caaaggttta actcatatac aaatcgaata
     2641 gaaatgcctt atttaagatc accgattgta catcacgtac atccacaacc gttccatcta
     2701 aaacaattcg ctaaacagca ccaatataca aatgcttttt atggtcctta tcaacgacct
     2761 gaattgacaa tgtttatgga tcatattgca catgcttcta aacaatatca attagaggat
     2821 tgcttggttc aaggtttagt tcaaacttta gataaacaag aagacaaatg gcatatcaag
     2881 ttagaagatg gacaaattat cactacagat tgcgtcgtta ttgcaatagg cagtacaaat
     2941 attccgttta tgcctgacat tttaaaagac aaacagaatg taaatcatat cttcgagaaa
     3001 gaacttgatc aagtagtata tgataagacc gatcatatcg ttggtagcgg cattactgct
     3061 gcacatcttg cacttaaatt gttaaatcat gataacgata aaaagattca tttatggcta
     3121 aataaagata ttgaaataca tgactttgat gctgatcctg gttggttagg tccgaaaaat
     3181 atgtcttcat ttttaagtac taaaagcatg cctgaaagaa atgccattgt acaacgcgaa
     3241 cgtcataaag gatcaatgcc tcacgaactg tacttacgcc ttaaaaaaca tattaaaaat
     3301 ggtcgtataa atgtgcataa aacacctatc actcaaatta gtggtggtgt aattaacact
     3361 gaaaatgatt ctgttccata tcaacagatt atggttgcaa ctggttttga acaagatttt
     3421 atgtcacaac cacttattaa gcaattaata caaaattatg atgcacctat caacgaatgt
     3481 aattaccctg ttatttccga aaaattagaa tggataccaa atctatttgt cgcaggatgc
     3541 tttgcagact tagaattagg accatttggt agaaatgtta tgggtggccg taaagctgcc
     3601 gaacgcattg aacaagcatt tctaaaacta caacaatata gcgcataaaa aaactgctag
     3661 ccgtagttat gaatatctca taacatttca gctagcagtt cttttatttt aaaggtttaa
     3721 tagcaactgt accacgcata actgtaatta attgttcttt gtcattctta atttttatat
     3781 cccatacatg tgtcgactta cctcgatgaa taatttcagc tgtcgcagta acacgaccat
     3841 ctttagcaga atgaatatgg ttagcattca tctctaaacc taatggaata aatttggttg
     3901 tatcaattaa attagcagat cctaatgaac atgctgtttc acctaaagca atcgaagccc
     3961 caccatgtaa atatccaaat ggttgtttta ctttatcagt aacaggcata gaaataacaa
     4021 ctaaaccatc ttcctggtga tctattgaca tctcaaatgt ctctaataaa tgagtcatat
     4081 tttcacctct ttatgcttat agtaccatag cagcaatagt accgaaaatc attaaaggaa
     4141 tattataaaa caagaagttt ggtacacatg tatcacgtat atgatcatgt tggccatcaa
     4201 catttaatcc cgcagttggt cctaatgttg aatcacttgc aggcgaacct gagtcaccta
     4261 atgcactcgc tgttccaatc aatgcgatta atgccattgt atctagtcca attgacgctc
     4321 caaaaggaat gaataatgat gcgataatag gaattgttgc aaatgatgat ccaataccta
     4381 aagtgacaat taaacctatc acatacatca tgataatgct aaataattta ttatcaccag
     4441 taatacttgt taaagtttta actaattcat ctatatcacc agtagcattc attacaccag
     4501 caaatccatt tgctgttaaa ataactacac caatataagc cataatttta ataccttcaa
     4561 caaacttagc atctaattca taccaattat atgcacgtga aataaagaat acgagtaccc
     4621 ctgccagtgc accaaaaatc attgaatctg taaatgtttg tactaaaaat gtagctagta
     4681 ttgctacaat tgttactatt aagatatatg gttttaactc tgtaacattg tcactatctg
     4741 aaattttacg tgtttcatat tcacgtggtt tacgatatac atataaaccg ataagtaagc
     4801 caacaatata ccccattgaa ggaataagca ttgctttcca aatcatatta aactcaattg
     4861 ggtgatttgc ctttgcaaag ccactttgaa taatttgctg gaaaatttga ccgaatccat
     4921 atggtaataa cacatacggg aaacataaac caaaaccgat aatcaaaccg attaaacgtc
     4981 tatctatttt taagtcatta aacagactta acaatggtgg gatgacaatt ggaatgaatg
     5041 caatatgtac aggaattaag ttttgactca tgatactcat agctaataat gcaatgatta
     5101 ttgtcacttt gactttaact cttgaccatc gactattttc agcgtgaatt gcatttataa
     5161 tttttcctac taaatagtct gtgataccac tgtatgaaat taatgctgca aatccaccta
     5221 ataaagcata gcttaatgct acctcagcac catcgactat atttttccca aatacattta
     5281 taactttttc aatgctcatg cctgaaatta agccaccaac tagcgcactg ataaataagc
     5341 ttataactac gtttaatcga cataaacata gcacaatcat taaaattact gctattacta
     5401 ctgcatttat catatgacaa ccatccttta tcactctatc gaactaaagc gtttatagac
     5461 tacatagtat cagtagcttt aacacctgtc aatagcaaac tataccaata atctttattt
     5521 tttagttaat tgt
//
LOCUS       DAKTIP010000171         5444 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.173, whole genome shotgun sequence.
ACCESSION   DAKTIP010000171 DAKTIP010000000
VERSION     DAKTIP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.173"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..355
                     /locus_tag="O0305_002262"
     CDS             <1..355
                     /locus_tag="O0305_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0275379.1"
                     /translation="YLKVKYDEKEKQKAIYYKEQQERITLYLKHNTKEPNTIKTVHFT
                     SLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKS
                     PDEIKKELESKKNDR"
     gene            622..1041
                     /locus_tag="O0305_002263"
     CDS             622..1041
                     /locus_tag="O0305_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0275380.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYLKVKYDEKEKQKAIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNSETSPMGSAVIEGYINENKKDNFTAYATPEHNYQ
                     FGGAMIESEKLSELLKPANQLKSPDDIKKELNKKKSH"
     gene            1059..1478
                     /locus_tag="O0305_002264"
     CDS             1059..1478
                     /locus_tag="O0305_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0275381.1"
                     /translation="MSKKHVFIIIGVILCICTVSTVMHFKMKYDEKEKQKAIYYKEQQ
                     ERITLYLKHNTKETNTIKSVHFTNLETSPMGSAVIEGYINENKEDDFTAYASPEHNYQ
                     FGGAMIKSEGVDKLLKPAHERKSPEKIKEELDKKEGH"
     gene            1930..2352
                     /locus_tag="O0305_002265"
     CDS             1930..2352
                     /locus_tag="O0305_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCY0275382.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
     gene            complement(2464..4002)
                     /locus_tag="O0305_002266"
     CDS             complement(2464..4002)
                     /locus_tag="O0305_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCY0275383.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            4235..5053
                     /locus_tag="O0305_002267"
                     /pseudo
     CDS             4235..5053
                     /locus_tag="O0305_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         1987 a          812 c          969 g         1676 t
ORIGIN      
        1 ttatttaaaa gtgaaatatg acgaaaaaga aaaacaaaaa gcaatatact ataaagaaca
       61 acaggagcgt attacactct atcttaagca taatactaaa gaacccaata ccatcaaaac
      121 tgtacatttc acaagtttaa aaagaggacc tatgggcgat gcagtaattg aaggttacat
      181 aaatgaaaat aaagaagatg attttgttgc atatgggtct ccagaacata attatcaatt
      241 tggtggaagt ttaatcaaaa gtaaaaattt aagcacgtta ttaaaaccag tacatcaaac
      301 caaatcacct gatgagataa aaaaggaatt agagtctaaa aagaacgacc gctaaaacta
      361 tgcggccgtt ttaattttgt tttcaagtta atcatactta catataaaaa tgaaaagttg
      421 aatttttagt acaatttcca ctgtatttat ctgttaacca ctttataccc gcaatatttc
      481 gttgatctta tataaacact tttggctgct tttccttgac ctagattaaa attatagatt
      541 ttctttgtaa gagtgtgttt tgtagtatca taaatatgta aactgtataa aattccacca
      601 cattttagga gctggtgata gatgtcgaaa aagaaaattt taatattaac tagtattatg
      661 ttaatcatat taattagtgt tgcaggtatc tatttaaaag tgaaatatga cgaaaaagaa
      721 aaacaaaaag caatatacta taaagaacaa caagcgcgca ttacacttta tctcaagcat
      781 aacactaaag aaccgaatac aatcaaatct gtacatttca caaactcgga aacaagtcct
      841 atgggaagtg ctgtgattga aggttacata aatgaaaaca aaaaagataa ttttactgct
      901 tatgctacac cagaacataa ttatcaattt ggtggtgcta tgatagaaag tgaaaaatta
      961 agcgagttac taaagccagc caatcagtta aaatcaccag atgatataaa aaaagaacta
     1021 aataaaaaga agagtcacta aagttaggag ttactttaat gtccaaaaaa catgttttta
     1081 taattattgg tgtcatattg tgtatatgta cagtttctac ggtcatgcat tttaaaatga
     1141 aatatgatga aaaagaaaaa caaaaagcga tttactacaa agaacaacaa gaacgtatta
     1201 cactctatct taagcataat actaaagaaa cgaacacgat taaatctgta catttcacaa
     1261 acttggaaac aagtcctatg ggaagtgctg tgattgaagg atacatcaat gaaaataaag
     1321 aagatgattt tactgcttat gcatcgcctg aacataatta tcaatttggt ggcgctatga
     1381 taaaaagtga aggagtagat aaattattaa aaccagcaca tgaaagaaaa tcaccagaaa
     1441 aaatcaaaga agaattagat aaaaaagaag gccactaggg tcttctttat ttttgattta
     1501 atcttccaat aatctatgtc attgctatcg aaggtgtttc gcaattaata taaatcactt
     1561 catcatcacc aatacttccc cagttttgta cagtacatta acacaaacga accacgttaa
     1621 tttaaatgga ataatagttt ggccattata agaacaatat atatcgaata acaatgtcga
     1681 tgacgtgatt tattgacatt atcaaatttt ctcaaatgtg tgtgattttt tataaaaaag
     1741 ggtagagcaa ggtataatat cctttcaatc tgttttcata ttttatatat tttttaaata
     1801 taagtgctaa atgttttaac taaagcatag attgacaaga tgttatacag aatttcaaat
     1861 tctatccaat attgttcgaa gtgtagtatc actggattgg tattaaacaa tgtaaaggag
     1921 agattgcaaa tgccgtataa ttacaagaaa caaaatggag agttaatgtc tgtaatgagc
     1981 caaggtgaaa agtttattca tcaatcaccc gttaatgatg aacttagtgc attgattaag
     2041 ttattaattt ctaaaattaa cggttgtcat tattgtgttg atatccataa aaaagaatta
     2101 aaggaattgg gtgtaacaca aatgaaaatt gatgaagtct tgagttttag acatttagat
     2161 ttatttactg atcaagaaaa agtgacgctt gaatttgcag aaatgttaaa ttcaatcaaa
     2221 gactttaaga agtttgaaat tattgaccgg ctaaaatcat tttatgatga agaacaaatt
     2281 attgatcttg tctttgttgt aaaccaaatt aacggttgga acagattaaa tattattagt
     2341 gatagactat aattgttcat ataaatgcag agtttcatct cgaacgctat atcataacaa
     2401 atcatgccac tatacaggtc aaatcttgta tagtggcatt ttaatttatc cctttgaata
     2461 ctgttattta acgaatatcg gtccacctgg tccaacaggg atacctaata ggaaccaaat
     2521 gatgacaaat actgtccata caatacttag cgcgattgaa tacggcatta aactagaaag
     2581 taaggctccg agtttcatgc gtttatcgta tttttgtgca taagttaata ataaaggtaa
     2641 gtacggcatc atcggtgtaa ttggattggt aattgaatcg cctacacggt aaatgacttg
     2701 tgtgaatgcg ggatgaaagc cgataaggat taacattggt acgaatatcg gtcctaaaat
     2761 accccattta gccgatgcgc ttccgattaa catgttgacc attgcactca gtacaataat
     2821 acctagtatc aatacaatac cgttttgatg ttctaataat ttggcacctt taacagcagc
     2881 gataattcct aaattactcc actttaaata cgcaagtagc tgtgctgcaa aaaacacaat
     2941 aacgataaat gttcccattg atcctacagc atcgccgaac attttaccta agtcttttgt
     3001 atttttaatt tctttgctta aaatcccata aactaatcca ggtactaaaa atacgacaag
     3061 aataattaat ccgacaccgt taattaatgg cgcatcgtct agtaagctgc ctgttttagc
     3121 atttcttaaa aagctatgtt caggaatggc tgtaataatt aataaaataa ttgtgactat
     3181 gaaactgata tttgcccatt ttaaagcatg tgcttctttg tcagtaatat gtgaagatgt
     3241 ttcttcatgg tcatcatgca ttaaactgtc atcatatttt cctaatctag gaataatgag
     3301 cttagttgta actagcaata tcgtaggaag taatacaacg acactcgctg cgataaagta
     3361 ccagttcata gcaacgtttg ttttaataga atctgaaacg atacgtgttg ccggttctgt
     3421 aaatgaatag accaaagcat cttgcatacc aacaactata tttgctgcaa atcctccaac
     3481 agcggaagca tatgccatcg ttagtccagc gatagggtga tagccaattt taataaaaag
     3541 cattgctgca agcggcggca agataattgt cgcagcatcg ccggctgtac tacctaaaat
     3601 accaattaat ataatagtcg gtaaaattaa gaagcgtggt gcgcgattca caacagaaat
     3661 cattaactta tcgaagtatc ctgttttctc tgcaacacca ataccaatca tcactgctag
     3721 tactaagcct aatgctggga actctgagaa atttttaatc gtatcattca ttatcatcgt
     3781 aaatccatca tggctaatta tatttttaat ataaatggtt tgatgcgtac ctggatgctt
     3841 aacagataca ttaaataatg agataaccca tgtcataatg gctaagccca cacacattaa
     3901 aaagaataag acgctaggat ctggcaattt atttccgatt ttttcaacac tattcaagaa
     3961 acgattgacg atagacccct tttgttgatg ttttgatgtc atcaattatt cccccctttg
     4021 ttaaatattt aaagtgtaac aaaaaatact ctcaaaagta acaattttca gaaaataaaa
     4081 aaactaatat tgttaaatat tttgagttat tcaatagaaa gtgtatagca gagtagttaa
     4141 gactgcctga agacttatct attaggttta tgaagcatcg aacagtggaa aataaggact
     4201 gtaagtttaa gatatgttgt ataggagtga ctgaatgaaa cgtttggaaa ataaagtagc
     4261 agtcgtaaca ggagcaagta caggtatcgg tcaagcttct gcaatcgctt tagctcaata
     4321 aggtgcgtat gtattggcgg tagacatagc tgaagcggta tcggagactg tcgataaaat
     4381 taaaagtaat ggtgacaatg cgaaggcgta taatgtggat aatgcaagcg aacaacaagt
     4441 ggtagacttt gtgtctgaca taaaggaaca gtttggaaga atcgatgtgt tgtttaataa
     4501 tgccggtgtg gataatgcgg ctgatagaat tcatgagtat ccaatagatg tgtatgacaa
     4561 gattatgaat gtagatatgc gtgggacatt tttaatgacg aaaatgatgt tacctttaat
     4621 gatgaatcaa ggtggctcta ttgttaatac gtcttcattt tccggacaag cagcagactt
     4681 gtatcgctct ggatataatg ctgcgaaagg tgcagtgatt aattttacaa aatcaatcgc
     4741 aattgagtat ggccgtgata gcattcgagc caatgcgatt gcaccaggta caattgaaac
     4801 cccgttagta gataaactga caggtacgag tgaggatgat gcaggtaaaa catttagaga
     4861 aaatcaaaaa tggatgactc cgctgggacg tttaggtaaa ccagaagaag ttgctaaatt
     4921 agtagtcttc ttagcatctg acgacagttc attcataact ggagagacga ttcgaattga
     4981 tggtggtgtg atggcttaca catggcctgg cgagatgtta agtgatgatt cgtggaagcg
     5041 gacattggaa taattattta aaatcgatat ccagtggaaa agaatttggc atgaagtagg
     5101 caatgtatgc atattgtcgc aatgaaagac atttaaagcg gagattaatt aatctttgaa
     5161 aatcacatat tgttcatttg aagtgtattg cttaaagctt gttttaacgt attgtaggtc
     5221 gtgcatctaa tttatcagaa tctcgagttc aaacttttgg agtatcttta aaataagctg
     5281 tattttatct attctaataa attaaggaga attttatgtt aaaagaaaaa gaaagtttta
     5341 gattgctata tcaagctata agagagattg cagataaaat tggagataat cagttagaaa
     5401 ctaattccgt tagtttatta ttattggact ttgattttga acat
//
LOCUS       DAKTIP010000172         5407 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.174, whole genome shotgun sequence.
ACCESSION   DAKTIP010000172 DAKTIP010000000
VERSION     DAKTIP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.174"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            135..1514
                     /gene="uhpT"
                     /locus_tag="O0305_002268"
     CDS             135..1514
                     /gene="uhpT"
                     /locus_tag="O0305_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCY0275384.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(1873..2631)
                     /locus_tag="O0305_002269"
     CDS             complement(1873..2631)
                     /locus_tag="O0305_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0275385.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(2624..4180)
                     /locus_tag="O0305_002270"
     CDS             complement(2624..4180)
                     /locus_tag="O0305_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCY0275386.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     NRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(4177..5145)
                     /locus_tag="O0305_002271"
     CDS             complement(4177..5145)
                     /locus_tag="O0305_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCY0275387.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
BASE COUNT         1665 a          836 c          992 g         1914 t
ORIGIN      
        1 attttagtac ataaatatgt ttctaagtat gtgtttatgt tcagtatttt ggataattta
       61 ataattttaa ggatattaag cgcttacacc gacgtgatat atttggctta acgaaaatga
      121 ttgaggtgac agagatgaac ttttttgata tccataagat tccgaacaaa ggcattccat
      181 tatcggtaca acgtaaatta tggcttagaa acttcatgca agctttcttc gtagtgttct
      241 ttgtttatat ggctatgtat ttaattcgaa acaactttaa ggcggcacaa ccgtttttaa
      301 aagaggaaat tggattatct acattagaac ttggttatat cggattagca tttagtatca
      361 cgtacggttt aggaaaaaca ttacttggat attttgtcga tggacgtaac acaaaacgta
      421 ttatctcgtt cttacttatc ttatctgcga ttacagtttt aattatggga tttgttttaa
      481 gttactttgg ttctgtaatg ggattattaa ttgtactttg gggacttaac ggggtgttcc
      541 aatcagttgg tggacctgca agttattcaa cgatttcaag atgggcgcca agaacgaaac
      601 gtggccgata cttaggattc tggaatacat cacataatat cggtggtgcc atagcaggtg
      661 gtgttgcact ttggggtgct aatgtattct tccatggaaa tgttataggg atgttcattt
      721 tcccatcggt gattgcatta cttattggta tcgcaacatt atttatcgga aaagatgatc
      781 cggaagaatt aggatggaat cgtgctgaag aaatttggga agagccggtc gataaagaaa
      841 atattgattc tcaaggtatg acgaaatggg agatctttaa aaaatatatc ctgggaaatc
      901 ctgttatatg gattctatgt gtttcaaacg tctttgtata cattgtacga atcggtattg
      961 ataactgggc accgttatat gtgtcagagc atttacactt tagtaaaggc gatgcagtta
     1021 atacgatatt ctactttgaa attggtgcat tagttgcaag tttattatgg ggctacgtat
     1081 cagacttatt aaaaggtcgt cgtgcaattg tagctattgg ctgtatgttt atgattacat
     1141 ttgttgtctt attctacaca aatgctacaa gtgtcatgat ggttaacatt tcattgtttg
     1201 cattaggtgc gttaatcttt ggtccgcaat tattaattgg tgtatcattg actggttttg
     1261 ttcctaaaaa tgccatcagt gtagcaaacg gaatgacagg ttcattcgcg tatctattcg
     1321 gtgactcaat ggcgaaagtt ggtttggcgg ctattgctga tccaacacgt aacggtttaa
     1381 acatctttgg atatacatta agtggatgga cagatgtttt catcgtcttc tatgttgcat
     1441 tattcctagg catgattcta ttaggaatcg ttgctttcta tgaagaaaag aaaattagaa
     1501 gtttaaaaat ttaatataaa tcggattaaa agtatcgcca atctattgca atatagttgg
     1561 caatcctgcc ccgacggcat gtgcgtgaag agatgaaaga tactgcttct acccttgcaa
     1621 atatatcatc tctatgtctc ggggcagatc ataattccct gttatgaagt atccttattt
     1681 gcccgactta gggtgactca atgaatttac tccttacaat aaagacatat agcggtgtca
     1741 atattgtagg gagtattgtt ttatatttaa actctctaaa aagcggactg aaagaaaagt
     1801 gaaaacttct ctatcagtcc gctttttcat agaacaaaat ggaggcgcca taatcattag
     1861 ttatgtgcta atctattttg cttgcttaca ataatcactt ggcgacattt gtaaatattt
     1921 tttaaaatga tagctaaaca ttttatactc tgaaaagcct actttgtctg caatttcata
     1981 gtgtttgtaa tgtcgatcta acaattgcag agattgtaaa atacgatagc gatttaaata
     2041 atcgacaatt gtaataccaa catgatcttt aaatgttcgc atcgcatacg attcactaac
     2101 atcgatatgt tgaattaaat ctgaaacagt cactttcgtt tgataagatt gcttaatttg
     2161 atccacaatc tggtttacat aataatcatc gtattctact tttaatagtg gttggaaggc
     2221 atcatgacaa gatgctaagc tacggccgtt ctgtgattgt tgctctaata aggtacggac
     2281 aagtcttcct aaaataactt ctaattgtgc atggtctact ggttttaata aataatcaag
     2341 aacatgatgt tgaatgccgg ctttcatata ttcaaagtca tcgtaactcg ataatatgat
     2401 gacattacaa tctagatgcg caatatcatt gagtaaatcg acgccatttt tacgtggcat
     2461 acgaatatca gtaattacta attctggctg atgttgttga attagtgata atgcttcaac
     2521 accatcttta gcagtgtata ttgtattgaa atgatagtct ccccaaggaa tgatttgctt
     2581 taatccttct cgaataattc gttcatcatc acaaataact accttaaaca tctacattcc
     2641 cccttgaaag tggtatttta taacaaatta acgtaccttg attacgcttt gaaaaaatat
     2701 ggagtcgtgc atgtgaacca tattgaatca ttgctttatt gtgtaaatga tttaatccca
     2761 aatgcttagt atcaaataca tcattattaa gagattggcg tacatattgc aggcgagatg
     2821 acgacatccc gataccattg tcgcaaacta aaacatgtaa attctgacgt gccaatgtca
     2881 ggcgtatagt aatgtccaat gactcagtat ctctaccatg tttaatagca ttttctatga
     2941 gtggctgaag catcatttta ccaattgtct ggtgacgcgc ttcttcagaa ctttcaatat
     3001 ggagcttaat catgtcatca aaacggatgt tttgtattgc aacatactgt tcaatgtagt
     3061 tcaactcttc gtttaattcc actgtatgtg agtttgtacg taatgagtaa cgtaacattt
     3121 gcgataattg ttggaccaca gtttgtgcta atttcggaga taacgtaatt aaatattgta
     3181 ttgtttgcat cgtattgaat aggaaatgag gctggaattg gcgttctatt tcctttaact
     3241 gaatatcacg caagcgacgt tctgtatgct cgatagaatg gatcagttgc tcatttgatt
     3301 caaataaatc gtaaatataa ttattaattt cttctagttc actgttgttt tttaaaggcg
     3361 tatatgtacc tagatgacga tttttggcat agtaaatttt ttgaataatc gtttcgatat
     3421 cttttgtttg tcgtttagcc atattatctg cgctaatgaa accaaatatt actagtaaaa
     3481 caagaactac ggccataaca attaacaacg tgataccatc ttcaatgttt tcatgtatat
     3541 ctttataaat aatgagacga tggtcagcat ggtttaattt tacagattca ttcataaatc
     3601 cgaattgttg tggtctatac ttttcaccta tagtaaaacg gtcatcgttg gcgtataaaa
     3661 tattgtcata ttgatcaacg ataagtgcga attgtcggtt atctttctta atttcactta
     3721 aacgtggggt gttagccata taaattttaa gcatatatgt actatttttg aatttaagct
     3781 gatgcgttga aaataaatac atatttttag tgtttaaatg ttcataatta ttggttataa
     3841 actgatttgg tccagataat tcataataaa gtgttgcggg ctgttggtgt attaatttta
     3901 ataattcacg ttttgtagcg gtcacatcat gatgatttgt taaatcgagc tcttgaaacg
     3961 aattattatg ctgtgtaata aatgtctgaa tctgcttttc agtatgatgt aaagatgact
     4021 gactttcatc aacatgttga tgaatcgtac gatgctcaat ccaaatatag atggcataga
     4081 agcttactag tccaataata atgactaaaa atactggaaa aatagtagac gcaaataacg
     4141 atcgtcttaa ttgatgtcta taaggtttgt atgccgtcat tgaatcatct ccaaaaattt
     4201 atgatgtgga atatccggta atttagattt cggtattaaa ggtatgttct taagattttc
     4261 gatagactga tcgctttgtt cactaacatc ctttcgaatt gacttggcat cgaactctgc
     4321 aactaatcgt tgttgtactg agcggcttgt taaatattgc actaactttt tacgcttagg
     4381 atgagggtgt gcatttttaa ctaaagcaat accatcaaca tttaacattg ttccttcaat
     4441 tggataaacg attgatacag gataaccttt gtttttccat gtgcgtgcat cttgttcgta
     4501 gcttagacct gcgtaatatt taccttttgc aacatcttca atgactttag acgtctttga
     4561 cagttgcatc gcatggtttt ggaattgatg cacatcactt actcgatgat gcatgctata
     4621 aatagcacgc atatgttgat agcctgtcgt tgttgtattt ggatttgagt acgcaatttt
     4681 acctttaagt ataggttgta ataaatcttg ataacctcga atcttaatat ctccttgtaa
     4741 atctgaattc actactataa ctgttggcat taatagaaaa ctagtaacat atttattgtt
     4801 cgagcgataa tcctctaatt gctgtgttac agatgtatct tgatagggaa caaaatcttc
     4861 tggatgatca attgtttctg acaacacacc acccataaag acatcaccac gctccgaaaa
     4921 atcttcgtta tgcaagtttg aaagcagtac ttgagtagat ccgtgtttaa tttcaatttt
     4981 gacatgctct tgtttttcaa attcatttaa aattggacga atcaagtttg attgatacgg
     5041 agaataaact gttaatacat ttttatcgga ttcagagtga cgcgtattag cgcatgctga
     5101 taaaaaaatg agaaataata gcaagatata aatttttgat ttcatgatat cccatcaatt
     5161 ctatgtatat tttaatacaa taattttagc aataaatgac gcataagtaa tgttaaatat
     5221 ttagaaatgt ttatagatga cttgttaaga cgttgcaaat gttgtgatag cacaaaattt
     5281 ttgtttgtca agacgattta ccgaggctgt aaaatcaaac tgttatattt tatttgtagc
     5341 tgttatataa aaatcggcaa gatattgaac ggttcaaaag tgaattttta cgtcaataaa
     5401 agtattt
//
LOCUS       DAKTIP010000173         5323 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAKTIP010000173 DAKTIP010000000
VERSION     DAKTIP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.175"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            117..803
                     /locus_tag="O0305_002272"
     CDS             117..803
                     /locus_tag="O0305_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCY0275388.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKELNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYIDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            793..1452
                     /gene="nth"
                     /locus_tag="O0305_002273"
     CDS             793..1452
                     /gene="nth"
                     /locus_tag="O0305_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCY0275389.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1457..1798
                     /locus_tag="O0305_002274"
     CDS             1457..1798
                     /locus_tag="O0305_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275390.1"
                     /translation="MTNITKEVFDNLEQEIDLFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(2364..4547)
                     /locus_tag="O0305_002275"
     CDS             complement(2364..4547)
                     /locus_tag="O0305_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCY0275391.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(4544..5221)
                     /gene="recU"
                     /locus_tag="O0305_002276"
     CDS             complement(4544..5221)
                     /gene="recU"
                     /locus_tag="O0305_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCY0275392.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1798 a          836 c          890 g         1799 t
ORIGIN      
        1 tactgttatt taaaatttgt ttgctactag ttaaatatat taagcatttt agtcgaaaat
       61 taaagaaaat gatttatact attaagtaat ggttaaattt aaaagggggc acgaacatgg
      121 ataaatatca attaaaagca agacctgtag tgatacgaag agaattatta gaccattaca
      181 gtgacttagg tttagatgag caagatttag tcattttgct taaattgatt tatgcttccg
      241 aaacatcaaa taaacagcct tcaattgaat tgcttcagaa gggctcaact atgcaaccac
      301 gtgatattac aatggttatt caaaatttga tacaacgtga attgctagaa ttacaagttc
      361 aaaaagatga agaaggtaga tttaccgaat atatgaatct agatcctttt ttcgaaaaat
      421 taagtcatat attgaagcaa caaagtatgg aaacgaaaga gctaaatagt aaagaaaaat
      481 ttaaacaatt atttagagtt ttagaggata catttgcaag gccgttatct ccatatgaaa
      541 tagaaacgtt aaatcaatgg attgatgttg ataaacacga tactgcaatt atacaagcgg
      601 cattagacga ggcgaatagt ttgaataaac ttagctttaa atatatagat cgtattttac
      661 ttaattggaa gaaaaacaat gtcaaaacaa ttgacgattc tagaaaaata agagaaaaat
      721 tcaataaacc gaaaatgacg cacacagtta aaacggtacc taaatttgat tggttaaacg
      781 gggagaacct tgatggtaag taagaaaaaa gcattagaaa tgattgatgt tatagcaaat
      841 atgtttccgg atgcagaatg tgaattaaaa catgataatc cgttcgaatt aactattgct
      901 gtattattgt cagcgcaatg tacagacgtt ctagtaaata gagtaacaac tgagctgttt
      961 aaaaaataca aaacgcctga agattatttg gcagtcagtg atgaagaact tatgaatgat
     1021 attcgttcta tcggtctgta tcgtaacaaa gctaagaata ttaagaaact ttgtcaatct
     1081 ttgattgatc aatttaatgg agaaatacca caaacacata aggaattaga aagtttagca
     1141 ggtgtaggac gtaaaactgc taatgtagtc atgagtgtag catttgatga accttcatta
     1201 gctgttgata cgcatgtaga acgtgtttct aaacgcttag gtattaatcg ttggaaagat
     1261 aatgtgagac aggtagaaga ccgtttatgt tctgtcatac ctagagatag atggaatagg
     1321 agccatcatc aactcatttt ctttggaaga taccactgtt tagcccgaaa acctaaatgc
     1381 gatatttgtc cactattaga agattgtaga gaaggacaaa aacgttataa agctagtttg
     1441 aaagaagcgt gataacatga caaatattac aaaagaggta tttgataatt tagaacaaga
     1501 gattgattta tttgccaaaa ataagacatt aggttctagt gaagcaaagc cctacttgga
     1561 tgaataccat agtaaaatta ttgattattt taagcaggtt aatgacataa ctggcaatat
     1621 tgattttgat aaattaaatc aatatcctgt tgtgccaatg aattttaaag aaagatatga
     1681 ttatatgatt gaacgtaaat atcattttat gggctatcga caaatgaaga cctttaaaac
     1741 tgaattgatt aaaatgaatg ctagttatca aacaaggtta aagaataagc aggtatagac
     1801 aagattgatc atttcctaat gttgaaaata tataaaacta attcatgcac acatatttat
     1861 aattatgtta ggtgtcgtgt cattacaacg tcttaatata aaaactgtcc atctacatca
     1921 taatggatat cttgagatat gctttaagat atctatttcg aatcgtaggt ggatttctta
     1981 tatctatgat tgtgaaaata tgttgcaaac atgttgatat ttcactgatt aacgtacaca
     2041 gggaattaat aaatatatca atttaaaaca gcgtctaaga tgactatgag aaaatataat
     2101 cataatttta aaaatgggaa caaccactta ttcaaattca ataattttac atagctaaag
     2161 atgtgaatat gaaaaactat ttagatgttt caaaatgtat gtgaatgact gattttacgg
     2221 tttctactaa tatagcaata gcgtatgaat acttataaaa aaagcaggaa gttttacctt
     2281 cccaccataa aagatgaaga acctaaaaaa atcctcggct acaccataaa tatggtataa
     2341 ctgaggattt tatgttgagt ggattagttg aatatacctg ttaatccacc gctgtttcta
     2401 gattgttgag atctagtatt gttatttgat tgtgcagtac cactgctgtt tgctgatgtg
     2461 tcactaccac cgtgtgtact acgatttgta gtgttgttat cttgactacc agaaacattg
     2521 attgatggga tactaccact tactgagcta ggacgtttaa agtcttcgcc atctctagat
     2581 gaaatttttg acatcacatt ttcatataag aactgtggat attcttgttg gctatgtccc
     2641 acaaatgagt tttcaccata ttgtttaact ttactgaagc ccatccacac tgacatagtg
     2701 tattgaggtg taaagccgtt aatccacacg tctttcgctg cattatcagg taaattatat
     2761 tgtgaataag tttcagcacc gtaagtacca gtacctgtct tagcacccat atttactcca
     2821 gatacaccat ggccatatgc agaaccatat ggtttaaatg taccttttag catctcagct
     2881 aacatgtatg cagtgtaatc actcatcgct ttatggctag tatgatcgta ttcgattgtt
     2941 tcaccatcac gagtaactac tttttgaatt gaatgcgcgt tgttataagt accaccgtta
     3001 gcgattgcag caaatgctga tgctaattgt gttggtgaga attctgaagc agaaccacca
     3061 agtacttcag atggaccaat atcgccttcg tagtttaagc caagtttggc agcgaatttc
     3121 ttaggtgcat cattaccagc attttgctta actgattgcc aagcttttaa agctgggata
     3181 ttgaaacttt gtcgtaaagc atcataaata gatacagtac cgtgactctt cgtatcatag
     3241 tttctaaatg tagaaccatc aacttgatat gaagattcat cttgaatcgc atggtttgtt
     3301 gcccatttca tattttcaat ggcaggtcca tacgctaaga aaggttttaa agatgaacca
     3361 gtagggtgag gatctgttgc ttggtttctg ttaacgacgt ctttgaaatc acgtccacca
     3421 gatatagcaa ctaaaccacc agttttacta tcaagaattg ttgcaccaac ttgttggtct
     3481 ttattcttgt agaagctacc attatcaaca tcattttgta atgttttttg aacatcttta
     3541 tccatgtttg tataaatttt aataccactt tgtaatacat tacctaaatt ttcatctttg
     3601 aatgctttat tattcattaa ttcagatttt acaaagttaa cgtatgaatt atactcagaa
     3661 tcttgatttg tatcaatgtt ttgacgttct tcaggagtac gatttactaa gttcgctttt
     3721 aaatcgattt tcttagcatc ttcccactgt ttatctgtaa tgcgtttatg ataatgcatt
     3781 aagtataaaa cagtgttttt acgatcttca gcagcttttg gatgatcata aatattatag
     3841 ttgtttggaa cctgaggtaa accggctaaa taagcttctt ccgctaagtt taaatctttt
     3901 aaatctttat taaagtaata cttagcagca gctttaatac ctgttacgcc atcagaatag
     3961 taaattttgt ttagatatac ttggaagata tcatctttac tatactcttg ttctaaacga
     4021 tatgataagt atgcttcttg agctttacgt ccaatagatt tatgttgtga taaaaatgca
     4081 tctttaacaa cttgttgtgt taatgttgag gcaccttcag aaccaaatcc accagtcaag
     4141 ttcttaccaa ttgcaccgaa taaacgttta taatcaagtg cgccatgttc gtagaaacga
     4201 ttgtcttcag ttgcaagtac tgcgtctttc attgatttcg gcacgtcttt taaatttaca
     4261 tgctcatgtc tttggccatt atctaatgtt ttaacaagtt ctccgttctt gtcatatatc
     4321 tttgcaggaa tcggatcttg taatttagct tcggtaaaag caggtgcttt ccaagcataa
     4381 taagcaaaca ataagatacc tagtaaaaga acaacgaaaa atgcaataat catgaagcca
     4441 ataatcttaa taatcgttct cttcacattt ctattctttt ttgagttgga tttcccacca
     4501 ttattaccgt ttttcttagg ctgagaagat cctttgtttt ccgtcatacg cggtcctcac
     4561 tttcatctaa tatcaactta tcaactgctt ttagatagtc taatcttggt tgatactgat
     4621 aaggaatatg gtaaccattt tttcgtattt catcaactgt tatagacttt ttaatattat
     4681 ctttatatct cttccaaaat acttcgaatt ttgaataggg taaaagataa acttcatcta
     4741 gcgttttaaa acgaatcatt aaaaacacaa tacctttttg ttgatatgca tttttcatat
     4801 gttcgacttg atggtcatga atattattta aaggaaagga cgtcttgttt ttagtttcct
     4861 ttgcttcaaa atcaatataa taaccttgat aaacgccgtt gtaatcagtt gttgaaggtg
     4921 tacgaaaata agcttcgtta atcacagctt tactccgctt aggatagttg acattaacta
     4981 tttgtactgg cgtaggcttt ttgtgaataa ctgcaatgtc gcttttaaga taaaacgtat
     5041 ttgaatgttc gatatctttt tcaagtgaca taccacgtcc accatactca atattactaa
     5101 aggcagcggt ctttttccct ccgtctatag cactattttt acgatatggt ttaccatttg
     5161 gataattcat aaatttcacc acacaagttt ggtagtaaac gttgtttatt atacacaaca
     5221 ttatagtgaa acaccaaact taaattagta taaatgcgaa agacacaata caccaaagct
     5281 tattttaacg tgatttcagc attaacacaa taatattata ata
//
LOCUS       DAKTIP010000174         5312 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAKTIP010000174 DAKTIP010000000
VERSION     DAKTIP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.177"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(108..956)
                     /locus_tag="O0305_002277"
     CDS             complement(108..956)
                     /locus_tag="O0305_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0275393.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            complement(1026..1442)
                     /locus_tag="O0305_002278"
     CDS             complement(1026..1442)
                     /locus_tag="O0305_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCY0275394.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     VRDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            1686..1853
                     /locus_tag="O0305_002279"
     CDS             1686..1853
                     /locus_tag="O0305_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275395.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            1850..4273
                     /gene="hysA"
                     /locus_tag="O0305_002280"
     CDS             1850..4273
                     /gene="hysA"
                     /locus_tag="O0305_002280"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase HysA"
                     /protein_id="HCY0275396.1"
                     /translation="MTYRIKKWQKLSTITLLMAGVITLNGGEFRSVDKHQIAVADTNV
                     QTPDYEKLRNTWLDVNYGYDKYDENNPDMKKKFDATEKEATNLLKEMKTESGRKYLWS
                     GAETLETNSSHMTRTYRNIEKIAEAMRNPKTTLNTDENKKKVKDALEWLHKNAYGKEP
                     DKKVKELSENFTKTTGKNTNLNWWDYEIGTPKSLTNTLILLNDQFSNEEKKKFTAPIK
                     TFAPDSDKILSSVGKAELAKGGNLVDISKVKLLECIIEEDKDMMKKSIDSFNKVFTYV
                     QDSATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFNDKTQNDT
                     TLKSWIDDGFMPLIYKGEMMDLSRGRAISRENETSHSASATVMKSLLRLSDAMDDSTK
                     AKYKKIVKSSVESDSSYKQNDYLNSYSDIDKMKSLMTDNSISKNGLTQQLKIYNDMDR
                     VTYHNKDLDFAFGLSMTSKNVARYESINGENLKGWHTGAGMSYLYNSDVKHYHDNFWV
                     TADMKRLSGTTTLDNEILKDTDDKKSSKTFVGGTKVDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKANGYTLYTDDKQTTNSDNQENNSVF
                     LESTDTKKNIGYHFLNKPKITVKKESHTGKWKEINKSQKDTQKTDEYYEVTQKHSNSD
                     NKYGYVLYPGLSKDVFKTKKDEVTVVKQEDDFHVVKDNESVWAGVNYSNSTQTFDINN
                     TKVEVKAKGMFILKKKDDNTYECSFYNPESTNSASDIESKISMTGYSITNKNTSTSNE
                     SGVHFELTK"
     gene            complement(4333..5187)
                     /locus_tag="O0305_002281"
     CDS             complement(4333..5187)
                     /locus_tag="O0305_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCY0275397.1"
                     /translation="MNKTNFGAYLNKSKSRKIYKIFDQNLKREFSYWQFRKLFKSYTR
                     QSDVHELKVCTQNDGTIEQIWIDEANQFGVSVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEIFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNQGKVNTDYFC
                     FGKDIIAPANGTVEKVVNGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLHQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQAE"
BASE COUNT         1830 a          875 c          814 g         1793 t
ORIGIN      
        1 gcttgctcct ttcacttttt aagagtaaaa aataacctga aacatagact ttaacatcca
       61 agtttcaggt tagtgtaatg taaatgattt actttgttat attatgatta aacatctaat
      121 tctctgcttg taagtgcttt aatcatctct ggatcagcat gtgagaaaaa ttggctatta
      181 ctttcttcta atgtcgcaat ttgttgttta tcttcttctg taagttcaaa atcaaatata
      241 tctaagtttt gagccatacg ttctggattc actgattttg cgagtacaac aatgtcacgt
      301 tctactaacc agcgtaaaat aacttgtgca attgatttgt tgtatttatc tgcaatggcc
      361 tgcaacaatt gattttggaa taattgattt ttaccttctg caaatggtgc ccaagcttct
      421 accacaacat tttcttgttg aagtgctgca acttgttctt cttgttgatg gaatggatta
      481 atttctattt ggttcacttg tggttgaatt tgattgtgaa tacctaaatc tacaattcga
      541 tcaacgccga agttagatac accgattgct ttaattttac cattttcttt taactcttct
      601 aatgcacgcc atgagccata tacgtcattg taaggttggt gaataagcac tagatcaaga
      661 tagtccaaat ttaatcgttg taatgatcgt tcgattgatt tgatagtatc ttcatagtta
      721 acattttcaa tccatacttt agtcgtaatg aatagttcac ttctatcaat acctgaagct
      781 tcaatacctt gacctacttc agtttcattt aaataacttt gtgctgtatc aatatgtcta
      841 taacccgctt taattgcttc tttaaccgct tctgctgttt gttcttgagg aatttgaaat
      901 actccgaaac ctaatactgg tatctttaca tctttactta tttcaatatg attcataaaa
      961 tgaacctcct ttatcttgta tgtccactat aaaccatgga gcgaactcta tagcaagcca
     1021 taggtttact ttctctccca aagacgttca acattgtcat cataattatc actagcattg
     1081 attttagcaa tatgattatc ataattatca attttatatt gtagtaaatc tctcacatca
     1141 tgaattgtct ttaagttttc atttaattct tcgagttgct tatttaaaat ttgcttttgc
     1201 tgtgcttgaa tattttcatt tttaggaagc tgcgctaacc tgcaaaattc gataagcgat
     1261 tcaatactga cgcctgcatt tcgcaaattt ttcactaaat aaatccaatt taaatcacta
     1321 tcattatata ttctatatcc attttcatct cgattaactg gtggaatcac accaaccttt
     1381 tcataatatc ttaaagtgtc ttgagatata ttcatgagcg ctacgacttc tttagttttc
     1441 attgcgacta tcctttcagt tatgtttggt cgtctaaagt aatgttgctt tatatattgt
     1501 catcttcgtt tgaatacttc ttattttatt actcaaattt aaatttgtct cttttttaac
     1561 attttacatt tcatcgtttt taattacttt aaaaattgta taacttaaat atttaaaatg
     1621 atataatcac taagattgat aatatttaat tttttgaaaa ttattttagt tgcaattttt
     1681 gggtgatgcg gaaaatttgg gtgatcgatt gtttatatgc atcaacattc tttgtggatt
     1741 gtttgacagt agacagtcat ttgtatacgt taacgaattt tgaaaaattg aatcattatt
     1801 taatcataat attcattgcg cacgcgcaac acaaaaagga gacatgtcaa tgacatatag
     1861 aataaagaaa tggcaaaaat tatccaccat tacgttatta atggctggtg tgattacttt
     1921 gaatggtggt gaattcagaa gtgttgataa acatcaaatc gctgtggctg atacgaatgt
     1981 tcaaacgcca gattatgaaa aattgaggaa cacatggctg gacgttaact atggttatga
     2041 taagtatgat gagaataatc cagatatgaa gaagaagttt gatgctacag agaaagaggc
     2101 gacgaattta ctcaaggaaa tgaaaactga aagtggtagg aaatacttgt ggtcaggagc
     2161 ggaaaccctt gaaactaatt cttctcatat gactcgtacc tatcgtaata ttgagaaaat
     2221 cgcagaagcg atgaggaatc ctaaaaccac tttaaatact gacgaaaata agaagaaagt
     2281 gaaagatgcc cttgagtggc tgcataaaaa tgcatatgga aaagaaccag ataaaaaagt
     2341 aaaagaatta agtgagaatt ttactaaaac aactggcaag aataccaact taaattggtg
     2401 ggattatgaa attggaacac ctaaatcatt aacaaatacg cttatattgc tgaatgatca
     2461 attttcaaat gaagaaaaga aaaaattcac tgcccctatt aaaactttcg ccccagatag
     2521 tgacaaaata ttatcttctg taggaaaagc tgaacttgct aaaggcggaa atctagtaga
     2581 catttctaag gtgaaacttt tagaatgtat tatcgaagaa gataaagata tgatgaaaaa
     2641 gtctatagat tcatttaata aagtcttcac ttacgttcaa gattctgcca ctggtaaaga
     2701 acgtaatgga ttttataaag atggctctta cattgatcat caagacgttc catacactgg
     2761 tgcttatggc gttgtactct tagaggggat ttctcaaatg atgccgatga taaaagaaac
     2821 accttttaat gataaaaccc aaaatgatac aaccttaaag tcatggattg acgacggatt
     2881 tatgccactc atttataaag gtgaaatgat ggatttatca cgaggtagag ctatcagtcg
     2941 tgaaaatgaa accagtcact cagcatctgc aacagtaatg aaatcattgt tgagattaag
     3001 tgatgccatg gatgattcaa caaaagctaa gtataaaaag attgtcaaat cttcagtaga
     3061 gtcagattca agttataaac aaaatgatta tttaaattca tattcggaca tagataaaat
     3121 gaagtcttta atgacagata acagtatttc taaaaacgga ttaacacaac aacttaaaat
     3181 atataatgac atggatcgtg tcacctatca taacaaagac ttagactttg catttggttt
     3241 aagtatgacg tcgaaaaacg tagcacgcta tgaaagtatc aacggagaga atttaaaagg
     3301 ttggcacact ggtgctggaa tgtcttattt atataacagc gatgtcaaac actatcatga
     3361 taacttctgg gtgacagccg atatgaaacg tttatcaggt acaacaactt tagacaatga
     3421 aatattaaaa gatacggatg ataaaaagtc gagtaaaact tttgttggcg gaacaaaagt
     3481 tgatgaccaa catgctagta tcggaatgga ttttgaaaat caggacaaaa ctttaactgc
     3541 caaaaaatca tatttcatat taaacgataa aattgtattc ttaggaactg gcattaaaag
     3601 tactgattca tcaaagaatc cagttacaac gattgaaaat cgcaaagcga atgggtatac
     3661 gttatataca gacgataaac aaacaaccaa ttctgataat caggaaaaca attcagtctt
     3721 tttagagtcc acagatacca aaaagaacat cggttatcat tttttaaaca aaccgaaaat
     3781 aactgtaaaa aaagaaagtc atactggtaa gtggaaagaa ataaataaaa gtcaaaagga
     3841 tacacaaaaa actgatgagt attatgaagt aactcaaaag cattctaatt ctgacaataa
     3901 atatggatat gtgttgtatc caggcttatc taaagatgtc tttaagacaa aaaaagatga
     3961 agtaactgtc gttaagcaag aagatgactt ccacgttgtg aaagataatg aatcggtttg
     4021 ggctggtgtt aattatagta atagcactca aacttttgac attaacaaca ctaaagtcga
     4081 agttaaagcc aaaggtatgt ttatacttaa aaagaaagat gataacactt atgaatgtag
     4141 cttctataat cctgaatcta caaattccgc ttcagatatt gaatctaaaa tttcaatgac
     4201 cggttactct attacaaaca aaaatacgtc gacttctaat gaatccggcg tgcactttga
     4261 attaactaaa taagcaagaa aatctcgaca ctataccatt tttatagtgt cgagatttta
     4321 taagtatcgc catcattcag cttgtaatcc gcagaccaca tcccctttga taagttctcg
     4381 attatttata aatcgaattt ttaaagatgt acatgcttca atattcttat cattcattac
     4441 ttgaaaatgt atatgaggtt cagtagaatt gccagaattc ccaaccttcc caatgatatc
     4501 accatattta acattttgcc cctcattcac aatgattgaa tattggtgta aatgagctat
     4561 taagctatac tcattttctg cgtgcttaat tacaatataa tttcctaaaa actgtgattc
     4621 attcgtactt ccaatactgt tgtcttgaac cccattcact actttttcaa ctgtaccatt
     4681 tgctggcgca atgatatctt ttccaaaaca aaaataatca gtatttactt ttccttgatt
     4741 cttatatgtt ttatgatttt ctttaataac taaatcaata gcataccttt gatgtgggta
     4801 taaatagtga taattatata attcattatc tccaccccaa aaaatctcat agcaatctgt
     4861 tgtcggcact gcgtatttta aagtcgtttc attcttaatc gcatcataat taattaacgg
     4921 acgcataaca aatccgataa tactattatc tttccaagtc actgacactc caaattgatt
     4981 tgcttcatct atccatattt gctcaatagt accatcattt tgagtacaca cttttaattc
     5041 atgtacatca gactgccttg tataagattt gaaaagtttc ctaaactgcc aataactaaa
     5101 ctctcgcttc aaattttgat caaaaatctt atatattttc ctgctcttac ttttatttaa
     5161 ataagcccca aagtttgttt tattcatata tacttaacct attatattaa ttttggtatc
     5221 aattctttct ccagttcctg cgtcattttt gcgctacctg aatcgtctac acttaattgg
     5281 ataaatcaac atataaataa ttttttaaag ct
//
LOCUS       DAKTIP010000175         5047 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAKTIP010000175 DAKTIP010000000
VERSION     DAKTIP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.178"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..225
                     /locus_tag="O0305_002282"
     CDS             <1..225
                     /locus_tag="O0305_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCY0275398.1"
                     /translation="KQKIIEITDHGIGIPEEDQDFIFDRFYRVDKSRSRSQGGNGLGL
                     SIAQKIIQLNGGSIKIKSEINKGTTFKIIF"
     gene            509..3307
                     /locus_tag="O0305_002283"
     CDS             509..3307
                     /locus_tag="O0305_002283"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCY0275399.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            3321..4589
                     /gene="sucB"
                     /locus_tag="O0305_002284"
     CDS             3321..4589
                     /gene="sucB"
                     /locus_tag="O0305_002284"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCY0275400.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         1879 a          769 c          941 g         1458 t
ORIGIN      
        1 aagcaaaaaa taattgaaat tacagatcat ggaattggta ttccagagga agatcaagat
       61 ttcatttttg atcgctttta tcgagtggat aaatctcgtt caagaagtca aggcggtaat
      121 ggactcggat tatctattgc tcaaaaaatc attcaattaa acggaggatc gattaaaatt
      181 aaaagtgaaa ttaacaaagg aacaacgttt aaaatcatat tttaatcatg actgagacgt
      241 caatcaaagt cataggatca attttttaag tacacattag ctgtgactaa tgtataagaa
      301 caactataaa acaaataaac agtggttctt tatcatttct gttgtactcc caaaatttac
      361 aataaaatac atctataaac ctagaagaat caacgctttt gttgattctt ctttttagca
      421 gataaatagg taaatctact ttaacaaata actaaatagt gatattatta cattgtaagc
      481 gtttcaacat ttttgtggag ggtgtaaaat gactaacgaa agaaaagaag tttcagaggc
      541 tcctgtaaac ttcggtgcga atttaggtct aatgttagat ctatatgatg actttttaca
      601 agatccatca tctgtaccag aagatttaca agtcttattc agcacaatta agaatgatga
      661 ctcaattgta ccagctttaa aaagtacaag tagtcaaaat agcgacggca caattaagcg
      721 tgtcatgcgt ttaattgata atattcgcca atacgggcat cttaaagccg atatttatcc
      781 tgtaaatcct ccaaaaagga aacatgtacc taaattagag attgaagact ttgatttaga
      841 tcaacagact ttggaaggta tatcagcagg aattgtttca gatcactttg ccgacattta
      901 tgataatgct tatgaagcaa ttttaagaat ggaaaaacgt tacaaaggac caattgcatt
      961 tgagtataca catattaata acaataccga acgtggttgg ttaaaaagaa gaattgaaac
     1021 gccatataaa gtaacgttaa ataataacga aaaaagggca ctattcaaac aattagcgta
     1081 tgttgaaggg tttgaaaaat atcttcataa aaacttcgtt ggtgcaaagc gtttttcaat
     1141 tgaaggggta gacgcacttg taccgatgtt acaacgtact attacgattg ctgcgaaaga
     1201 aggtattaaa aatatacaaa taggcatggc tcaccgtgga cgtttaaacg ttttaacgca
     1261 tgtcttagaa aaaccgtacg aaatgatgat ttcagaattt atgcatacag atccaatgaa
     1321 attcttacct gaagatggta gcttgcagtt aactgctgga tggactggtg atgtgaaata
     1381 tcaccttggt ggcattaaaa ctactgattc atacggtaca atgcagcgta ttgcactggc
     1441 taacaatcca agtcacttgg aaattgttgc acctgttgtt gaggggcgta cgagagcagc
     1501 acaagatgat acacaacgag ctggggctcc gacgactgat catcataaag caatgccaat
     1561 tattatacat ggcgatgctg cttatcctgg tcaaggaatt aacttcgaaa caatgaactt
     1621 aggaaacttg aaaggctatt ctacgggtgg ttcattgcat attattacta acaatagaat
     1681 tggatttact acagaaccaa ttgatgcacg ttcaacaact tattctacag atgtggccaa
     1741 aggttatgat gtgccaatat tccatgtcaa tgcagatgac gttgaagcta ctattgaagc
     1801 aattgatatt gcaatggaat ttagaaaaga gtttcataaa gacgtcgtta ttgatttagt
     1861 aggttatcgt cgtttcggac ataacgaaat ggatgaacca tcaattacta atccagttcc
     1921 ttatcagaat attcgcaaac atgactctgt tgaatatgtg tttggtaaaa agcttgttaa
     1981 tgaaggtgtc atttcagaag atgaaatgca ttcatttata gaacaagtcc aaaaggaact
     2041 aagacaagct catgataaaa ttaataaagc tgataaaatg gataatccag atatggaaaa
     2101 gcctgcagat cttgcattac cgttacaagc agacgaacaa tcatttactt ttgatcactt
     2161 gaaagaaata aatgatgcat tgttaacata tccggatggc tttaacattt tgaaaaagtt
     2221 aaacaaagtt cttgagaagc gtcatgagcc gtttaataaa gaagatggtt tagttgattg
     2281 ggcacaagca gaacaacttg catttgcgac aattttacaa gatggtacac cgattcgctt
     2341 aactggtcaa gatagtgaac gtggtacatt cagtcatagg catgccgtgt tacatgatga
     2401 gcaaacaggt gaaacatata cacctttaca tcatgttcct gatcaaaaag cgacatttga
     2461 tatacacaat tctccgcttt cagaagcagc agtagttggt tttgaatacg gctataatgt
     2521 ggaaaacaaa aaaagcttca atatttggga agcacaatat ggtgattttg caaatatgtc
     2581 acaaatgatt tttgacaact tcttattcag ttctcgctca aaatggggag aacgttcagg
     2641 attaacatta ttcttacctc atgcatatga gggtcaaggg cctgaacatt catcagcaag
     2701 attagagcga tttttacaat tagctgctga aaataattgc acagttgtca acttatctag
     2761 ttcaagtaat tatttccact tattgcgtgc acaagcggct agtttagatt ctgaacaaat
     2821 gcgaccattg gttgttatgt caccaaaaag cttactgaga aataaaacag ttgcaaaacc
     2881 aattgatgaa tttacttctg gtggatttga gccaattttg acagaatcat atcaagcgga
     2941 taaggttaca aaagttattt tggcaactgg taaaatgttc attgatttaa aagaagcatt
     3001 agctaaaaat ccagacgaat cagtattact cgttgcgatt gaaagattgt atccattccc
     3061 agaggaagag attgaagcat tactagcaca attgccaaac cttgaagaag tgtcatgggt
     3121 acaagaagaa cctaaaaatc aaggtgcatg gttatatgtc tatccatatg ttaaagtgct
     3181 agttgcagat aaatatgatt taagttatca tggcagaatt caaagggctg ctccagctga
     3241 aggcgatgga gaaattcata aacttgttca aaataaaatt atagaaaatg cattaaaaaa
     3301 taactagggg gaaataagtc atgccagagg ttaaagttcc agaattagca gaatctatta
     3361 cagaaggtac cattgcagaa tggttgaaaa acgtagggga tagcgtagaa aaaggtgaag
     3421 ctattcttga attagaaact gataaagtta atgtcgaagt tgtatctgaa gaagcaggtg
     3481 tattatctga acaacttgca agtgaaggcg acactgtaga agttggacaa gcaattgcta
     3541 tcatcggcga aggtagtggc aatgcttcta aagaaaatag taacgacaat actccacaac
     3601 aaaatgaaga aacaaataat aaaaaagaag aaacaacaaa taattcggta gataaagctg
     3661 aagtaaatca agcaaatgat gacaatcagc aacgtattaa tgctacgcct tctgcgcgtc
     3721 gatatgctcg tgaaaatggt gtgaatcttg ctgaagtaag tccgaaaaca aatgatgtgg
     3781 ttcgtaaaga agatattgat aagaaacaac aggcaccggc atcaacacaa acaacacaac
     3841 aagcatctgc aaaagaagag aaaaaataca atcaatatcc tacaaaacca gtgattcgtg
     3901 aaaaaatgtc acgtagaaag aaaacagctg ccaaaaaatt attagaggta tctaataata
     3961 cagctatgtt aacaacattt aacgaagttg acatgacaaa tgttatggaa ttgcgtaaac
     4021 gtaagaaaga acaatttatg aaagatcatg atggtactaa attaggattt atgtcattct
     4081 ttactaaagc ttctgtagca gctttgaaaa agtatccaga agttaatgca gaaatcgacg
     4141 gcgacgacat gattacgaaa caatattatg atattggtgt agctgtttct acagatgatg
     4201 gattattagt accatttgta agagattgtg ataaaaagaa ttttgcagaa atcgaagcag
     4261 aaattgctaa tttagcagtt aaagcacgag agaaaaaact tggcttagat gatatggtta
     4321 atggttcatt tacgattaca aatggcggta tttttggatc aatgatgagt acgccaatta
     4381 tcaatggtaa tcaagctgca atcttaggca tgcattcaat tattacaaga ccaattgcga
     4441 ttgatcaaga tacaatcgaa aatcgtccaa tgatgtatat tgcattaagc tatgatcata
     4501 gaattattga cggtaaagaa gcagttggat tcttaaaaac aattaaagaa ttaattgaaa
     4561 acccagaaga cttattatta gaatcttaat ccacaacaca aaatagttta ctattcctaa
     4621 aagcgggatt aaatcaataa caaacagcag taagattatt tcctagtcga aatatcttac
     4681 tgctgtactt tatttttata atgattcgta ttaaaatgtg ggtttaattt tgactactta
     4741 aaaaatatat catttctatt gaaatagact cacaatagaa atataggtag aatagcgtgt
     4801 ttcaatatgc taatgaatgc attttagata ataacaaacg atgaaatata tgaattctat
     4861 aagcgccttt agttaattta aaatcatctg aacatgatgt aaattcgctt tatggacacg
     4921 acatatatat gtttttcatg tcctacaaaa aacgaaatat tcaattgcta tatacagata
     4981 ttcattaatg acacactcaa tagcaaccat aacaatatta tgttacctat ttaaatgaaa
     5041 tagttct
//
LOCUS       DAKTIP010000176         4973 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAKTIP010000176 DAKTIP010000000
VERSION     DAKTIP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.179"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(62..169)
                     /locus_tag="O0305_002285"
     CDS             complement(62..169)
                     /locus_tag="O0305_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275401.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKHSLI"
     gene            complement(206..529)
                     /gene="isdG"
                     /locus_tag="O0305_002286"
     CDS             complement(206..529)
                     /gene="isdG"
                     /locus_tag="O0305_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCY0275402.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(548..1282)
                     /gene="srtB"
                     /locus_tag="O0305_002287"
     CDS             complement(548..1282)
                     /gene="srtB"
                     /locus_tag="O0305_002287"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCY0275403.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIM
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(1344..2309)
                     /gene="isdF"
                     /locus_tag="O0305_002288"
     CDS             complement(1344..2309)
                     /gene="isdF"
                     /locus_tag="O0305_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCY0275404.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(2322..3200)
                     /gene="isdE"
                     /locus_tag="O0305_002289"
     CDS             complement(2322..3200)
                     /gene="isdE"
                     /locus_tag="O0305_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCY0275405.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(3197..4273)
                     /gene="isdD"
                     /locus_tag="O0305_002290"
     CDS             complement(3197..4273)
                     /gene="isdD"
                     /locus_tag="O0305_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCY0275406.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNQENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(4273..4956)
                     /gene="isdC"
                     /locus_tag="O0305_002291"
     CDS             complement(4273..4956)
                     /gene="isdC"
                     /locus_tag="O0305_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCY0275407.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
BASE COUNT         1499 a          868 c          637 g         1969 t
ORIGIN      
        1 attcttttct tcttcaaaat aaatcaaaat gatataattg atgattattt tcaaagcaca
       61 ttcaaatcaa actatgtttt agcaatttgt tgttagcatg tttgtgttca ttaaaaaaac
      121 gaccatcatc ggtatcatgt atggtcgtta caaaagctaa caataccaat tgtcataaca
      181 agtactgcaa cctctttaaa ttcaattatt tcatgtaact atagcctata tcatatgtaa
      241 ttactttgtt atttataatc gggctacttt catcttcatt tttacttcta acatgtttat
      301 gcgctgcttt aaagacatca gattttaacc aatccgtaaa agcttgcttt gatttccaaa
      361 ctgttaaaat tttcacttca tcaaaatctt cttgttctaa agtttgtgta acaaacatgc
      421 catcaaagcc ttctaatgtt tcaatcccat gtctcgtgta aaatcgttct ataatatctt
      481 ttgctgttcc ttttgttaac gtcagcctat tttctgccat aaatttcata attatcctct
      541 tttctgttta acttacctta attatttttg cgacaacaac aattcttttc gtcgtttcac
      601 tatatgcatc ttcgcacgtt gataaagtca ttattctatc ttttaccgtt acattaacat
      661 ctgaattaat tacagattta cgttttgtct catctaaaaa ttgttgataa tcttgatcat
      721 tttcaaaatc tgtacgtatg taattatctt tagtagtagt tttatatgca ctaaatactt
      781 gcaattgata tttaccatat ttattgtcaa attcaattat cttgtgtttt tcataaaacg
      841 attgctttaa ataatcttct aacacatcaa acatcgtatt atcaccgaca tggtgcccgt
      901 ataaaatagt attatgattt aaattcttca attcatttct aaaatccata aaaatactac
      961 ctttacgtcg atgttctcgc tcaaaatcta aatttaaata atcgtgattt gtcttacctt
     1021 gtagtactgg ataatttaat gatgttcctg ataattttat ccatccaaca atgtctttat
     1081 ttattttttc aagtgattca aattgtggtc tcacatgttc ttgatgtttg ctcatcagca
     1141 tttgaaattt ttgttgtaat ttctcataat ttgcgcgttc ttgcttgtct tcaatatatg
     1201 tttgaacaat tttgtaacca aaaatgataa taattacaac caataaaatt tgtacaatag
     1261 ttaaaaatcg cttcattctc ataaaaatcc tcttttatta acgacgtttc ttcagtcatc
     1321 actaaaccag ttgttgtacc gttttagatt cgatttcgtt gactttgaca aattaagtaa
     1381 attagcattg gaccaccgac aatcattaaa atagcattgg ctggaatttc taaaggaggc
     1441 tgtatcactc gtcctaataa atcagccact aacaatagcc atgcaccaat aactgtagaa
     1501 aacggaataa gtactctgta attgccccca actagctttc taaccacatg tggcacaata
     1561 atacctaaaa aggctagttg tccaacaatc gcaacagttg cacttgctaa aaatactgct
     1621 aataaacctg ttaaccatct gtaacgatca atattaaaac cgatacttcg cgcttgtatg
     1681 tcgtctaaat ttagtaaatt caatttaggg gacaatagta atgttaatat taatcccaat
     1741 aatgctgata ctgctaatat gtatacgtcg ctccatattt tcattgttaa gccttgagga
     1801 attttcatta aagggttttg agttaaaatt tctaaaacac catttaataa tacgaataac
     1861 gcaacaccta ctaatatcat acttacagca ttgaatctaa atttagaatg caacaatata
     1921 attattaaaa atggtattaa acctccaata aaacttaata atggtaagta aaagtacaat
     1981 tgtggaataa acaacataca aagtgctctc attataagtg cacctgagga aacgccaatg
     2041 atattcgcct ctgccaaagg attttgtagt gctgcttgta ataatgctcc agaaactgct
     2101 aacattgcgc caaccatcaa tgcaattaat atacgtggca atcgcaaatc aatgattgaa
     2161 tccactgctt cattgctacc agttgtaaat tttgtaaata ggtcattaaa tgacaattta
     2221 attgtaccgg ttacaaacga aatataagca gttgcgatta aaatgactaa caaacataaa
     2281 aatagtagtt tctttttatt ttttatcatc atatcaactc actatttttt atccttataa
     2341 aataaatcat ataattgagt cattgcctta tcagcatcaa catttgctgt aataccgaat
     2401 ggcacttcct ctaagtcata aacatgatta tttttcacag ctttaaaatg tttccaaata
     2461 tcattttgtt taaattcttt ttgaaacatt ttcttaactt cttcaggcat tccgtgtggt
     2521 aatcgtaaaa taatatctgg attgatattc aacaaatttt cagtattaga cgaaatatat
     2581 tgacgatctt tcactttaat aacattttct ccacctgcta ttttaactaa atcaccaata
     2641 tatgatttat cagttgctac taaatagcta cccggtacac ccattaatat taatactttg
     2701 ggatgtttct tttgtttagc tgctttattt tcaatttttt gctttacaga atttaaatgg
     2761 tcatttaatt cttttgcttg tgctttacga ttaaattgat cacctaattg tgtaatcgac
     2821 ttttgcatcc cttttaaact atcaaaatca taaaagtagc ctttcatatt taattgtttg
     2881 taaaatggtt gcatttcatc tttaatcgtt gacacactca aaacatgtgt tggttttaac
     2941 tttttaacag cttcaacatt cggctccatt ggttgaccaa tttccggtac atctttataa
     3001 cgattaggca atgtcttata tgacgtgggt ttgccgacaa ttggtaaatc caatttgtcc
     3061 aatgtcattg tcaatgcaac agttgttggt acgattctga attcgccgga tttagttgat
     3121 tcttgagaac tggaagattg acagctggtt aagataacga cgcttatcac taaaatggtt
     3181 aaatacttta tgattctcaa acttggttac ctctctttct gctgtataaa tgtgccgaaa
     3241 taagtaaaat agttgctaac acaatgccag ctgcaaataa catatattgc tttttatgat
     3301 ttggttcatc aagttcttta atatcactag ataattgact actattcttg ctatcatcat
     3361 ctctattaaa agctggtaac gctttttctt tttcaactac ttttttcttt tccttttttg
     3421 atttttcatc tttatgtttc ggttgctctt tgtgattatt ttcaaccttt tttattgttg
     3481 gagggctatt ttcaactttt aatgtacctg atgctttagg tcgctcattt tcaatctttt
     3541 tattactgtc ttgttgcact ttatcttttt gtacaggaat atcatttaat cctttttcag
     3601 taaaaaggct agtacgtctt tccttagtat ctgatttttg actatctttt ttgtcatcaa
     3661 tatctttttt aagctgatct tgattatttt cttgattttg atgttttgaa ggcacttctt
     3721 ttgcatcatc atgttgtact tttttctctt taaaatgaat agaatacacc atttctttgt
     3781 tatctagatt tttcttttca gccaacgttt taacatgcat attgatttta tccatatgct
     3841 gttcaattaa aattcttact attgtgtcat ccttttcatc ttttataaca aaaggaaatt
     3901 cttcattttt gtcattttga aaaacaagtt gttctttatc tatttgacct tttagcttta
     3961 cttgcaagta aattttacca gtcatatctg aaatcaattg cgccttttcg tcaactagtt
     4021 gttgttcctc agttacattt ttattttctt gattaaaaat tagtaactct aacgctttat
     4081 tctcttttat cttttcatca aaaattgatg tagttaattc tgttttatct tttgcttcag
     4141 gttgattaaa tttggctgat ggatatttta tctcatcagc caacacgcta ccaatcattg
     4201 ttgtatatgt aagtatacct aagctaataa tagctataat agattttgta gcaatttgtt
     4261 taacatttct cattattcca cattgccttt agattttttt ctaacaaaca atgtgattgc
     4321 gattaataat gctaaggatg caactggtat tgcatatata tatgcaggtg taccagcatt
     4381 tgtttgtgga ttttctactt tgtctttatt cgaactatta gattcacttt gaccagtagt
     4441 cgttccatca gatcctttgt cactacctga agcagaattt ttatcatctt tacctggtgc
     4501 attagcacct gctacatcag ttggtccatt aaatttatat gtaatgttgt aatgatggtc
     4561 atatttgaat ggctttccat ttactttttc atcgatataa acgtcaattt ttccatctat
     4621 tttaccgttc aacttactta cttcaaattc agaagtgcgt tcatctttgg cagtgttttt
     4681 actaataata ttttctttat gtccttcgat actcattcca gtaatccaat gactgtggtt
     4741 gacagttatt tgaacataca atttaccatt tttcttaatg tactttgccg gtttattaaa
     4801 atagtcatta gcaattgacg tgtcattggt attgtatttg taaacctcat aattcaaagt
     4861 accgctatct gcggcatttg cagaattact gaatgtcgcg atgatgataa ttaacgctaa
     4921 aatcgttgta ttaaaaactt ttaaaatatt tttcaaaaca taatcctcct ttt
//
LOCUS       DAKTIP010000177         4968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAKTIP010000177 DAKTIP010000000
VERSION     DAKTIP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.180"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..504
                     /locus_tag="O0305_002292"
     CDS             <1..504
                     /locus_tag="O0305_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCY0275408.1"
                     /translation="NLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINY
                     QIIETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDV
                     MTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELK
                     IRKSTQR"
     gene            520..2169
                     /locus_tag="O0305_002293"
     CDS             520..2169
                     /locus_tag="O0305_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCY0275409.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     GKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(2252..3118)
                     /locus_tag="O0305_002294"
     CDS             complement(2252..3118)
                     /locus_tag="O0305_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0275410.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(3348..4832)
                     /locus_tag="O0305_002295"
     CDS             complement(3348..4832)
                     /locus_tag="O0305_002295"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCY0275411.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         1680 a          767 c          870 g         1651 t
ORIGIN      
        1 aatttgacac gacatgttat tgaacaaggt gtagatgaat taatatttat tacagaaaaa
       61 ggaaattttg aagtttcaaa agatagaatt caaggatttg aaacgatagc ttctcaaaat
      121 aaaattaatt atcaaattat tgagactagt aatgaaagag aagttatttt taattacatg
      181 caaaatctac atacgcgttt gaaggaccca aatattaaac aggcaatcat ttcgttagat
      241 gctatgttac atttagcgat tttaagtgtc ctatatgaac ttaatattga aattccgaaa
      301 gatgtaatga cagcaacgtt caatgattct tatttaaccg aaattgcgtc accaccacaa
      361 acctgtattg acatcaagcc tcgaatgtta ggtcaacagg ctggtgcagc aattttaaat
      421 atattgaaaa acaaagcaca ggatgttatt gaactcgtca ttatagatac agaattaaaa
      481 ataagaaaat caacacagcg atagcaaagg agttttaaaa tgaataagca atggtggaaa
      541 gaagcagtag catatcaagt atatccaaga agttttaatg atagtaatca cgatggtatt
      601 ggggatttac ctggaatgat tgataaattg gactacttaa aagatttcgg tatcgatgtc
      661 atttggctca gtccaatgtt taaatcacct aatgatgaca atggttatga tattagtgac
      721 taccaagaga ttatggatga atttggaacg atggaagact ttgatcgttt attaaaaggt
      781 gttcatgata gaggcatgaa gcttatttta gatttagttg taaatcatac atctgatgaa
      841 catccttggt ttatagaatc caaatctagt aaagacaatc ccaaacgtga ttggtacatt
      901 tggcaagatc caaagccaga tggctctgaa cctaacaact gggaaagtat atttaatgga
      961 tctacatggg aatatgatgc taatactgag caatattatt tccatttatt cagtaaaaaa
     1021 caacctgatt tgaattgggg taatccggaa gttagagatg ctgtatttga aatgatgaac
     1081 tggtggtttg ataaaggcat tgatggattt agagtagatg caattacgca tattaagaag
     1141 acgtttgaag cgggtgactt acctgtacct gagggtaaaa catatgcccc agcatttgat
     1201 gtagatatga atcagccagg tatacaaact tggttacaag agatgaaaga tcgctcatta
     1261 agtaagtatg acattatgac tgttggtgaa gcgaatggtg taagccctga tgatgctgat
     1321 gactgggtcg gggaagaaaa tggtaaattt aatatgatat tccaatttga acatttggga
     1381 ctgtggaata gtggtgattc tcactttgat gtaaattcgt ataaatctgt attaaataga
     1441 tggcaaaaac aacttgaaaa taaaggttgg aatgcgttgt ttattgaaaa tcacgaccaa
     1501 ccacgacgtg tatcgacgtg gggtgacgat gacaagtatt ggtatgaatc agcaacaagt
     1561 catgcagctg tttatttctt gcaacaaggt acgccattca tttatcaagg tcaagaaatt
     1621 ggtatgacga attatccatt tgaaagtatt gaaacgttta acgatgttgc tgttaaaaat
     1681 gactatcaaa tagtgaaagc tcaaggtgga gatgtagacg ctttacttgc gaaatataaa
     1741 gatgagaacc gagataattc tcgcacacca atgcaatggg atgatacgtt aaatggagga
     1801 tttacaaatg gtgaaccgtg gttcccagtg aatccgaatt ataaaactat caatgttgca
     1861 caacaattag aagatgagca ttcagtatta caattttata aagatttaat tcaattaaga
     1921 aagtctaatg atgtatacgt atatggtcaa tttgatttag tagatgctga aaattcacaa
     1981 gtttttgcgt acacgagaac attaaatgaa aagcaagttc ttattgtagg taatcttact
     2041 aaccacgaag cagaattaac tgtaccattt gatttaagcc atggagaagt aaagctattt
     2101 aattatgatg ccaaagttaa tttaaaacag ttacgtccat atgaagcatg tgttatcgaa
     2161 ctaaattaac atattaatat gccccctaat agaatctaag gccatttgta gacctataga
     2221 tatattaggg ggcttctttg ttttttatta tttaatcttc gtcataagga ctctgtttaa
     2281 caggaatcca aagctgaatt ttagtgaatg ggtcattaaa tgaaatatcg agagggtaca
     2341 cttcaacata taaatcattt cgttcatatg gcaatgttaa ctgcaaactt gattcaatat
     2401 agtaccaagc ttcattagtt gcataatcaa tttcaccttg taaattgaat ttcgcacaat
     2461 gtttgccagg taaaaatcga ctttctaagt gtgcaggata acgttcactt ggtacaccta
     2521 caaatatttc taaaccattt tcaagaggac aactaataac aaatagttca aatggactaa
     2581 cgtcattata tcgtcgtaac tctttaattt taccatcaat gagcaagtct tctaaaaaat
     2641 ccggaacatt aaaaggatgt gacaaatact tagtgtctat aaatcgtgca tatccaacca
     2701 atgaaatatc atctgtctct tctaatctgt atggataagg tgctctctca gtagttgata
     2761 atttgatata taatcgctct tgaatttgta attcatcttt tttagtagag gcttgaatag
     2821 gtgatacgcc gtgaaaatca ctaaaatcat ttgcaaaatc atttgaattt gcatagtgat
     2881 attttttagc gatatctaca agtcgtgtag caccattaat cacatcattt gcagcgagtg
     2941 tcattttacg cgcgcgtgca taagcttctg gagataagcc gacaatcatt ttaaatgatt
     3001 gatcaagatg gtatggcgaa agaccaacgt aatcacttaa ttcttgcaaa ttgaaaggct
     3061 ctaataaacg atcttcaata taaacaattg cctgttgtat ttgcttgata acgtccaaaa
     3121 ctttcactcc aattaattca atcatttatt tttattctac attatttcta taaattatac
     3181 acccatttgt tcaatgatta ttaaaatagt tttgggcatt gtaaaatata atttcataat
     3241 atagtctaga aaaaaagcga atgatagaac aattgattta cttgattcgt aatcaatcct
     3301 tgtcattcgc tcatttattt ttgtttaaca tgtgcgtttt aattcaatta ttgaatatcg
     3361 tcccaccaat ggttaccatc acgagcaagt agtaaatcac tttctaatgg accattagta
     3421 cctgattcat agttagggaa ttctggatca accatattcc attcatcttg aattgcatca
     3481 acaaatttcc atgttgattt taattcttcc cagtgcgtga agttagtggc atcaccttta
     3541 agacaatcaa ataatagatt ttcatatgca tctacagtat tcattttatc ttgagcgctc
     3601 attgagtaag acaattggac aggttctgtt tcgatacctt gtgtattttt cttagcattt
     3661 agatgtaaag atacaccttc attaggttgg atattgatta ctaataggtt tgaatctaac
     3721 agtttatcag tttcatagta taagttcatt ggtacttctt taaattcaac gacaacttga
     3781 attgttttag atttcatacg tttaccagta cgaatataga atggtacacc agcccatcta
     3841 aagttatcaa ttgttaattt acctgaaaca aaggtaggtg tgttagagtc atctgcaacg
     3901 cgatcttcat cacggtatgc tttaacttgt ttaccatcga tatagccttc gccatattga
     3961 ccacgaacaa agttcttttt aacatcttca gattggaaat gacgcagtga tttaagtact
     4021 tttactttct cagcacggat atcttcacta tttaaactaa taggtgcttc catagctaat
     4081 aatgcaacca tttgtaacat gtggttttgc accatatctt ttagcgcgcc acttgattca
     4141 taataaccac cacgatcttc aacacctagt atttcagaag atgtaacttg gatgtttgaa
     4201 atatatttgt tattccataa tggttcaaac atcgcattcg caaaacgtaa tacctcgata
     4261 ttttgaacca tgtcttttcc taaatagtgg tcaatacgat aaatttcttc ttctttaaat
     4321 gatttacgaa tttgattgtt taatgcttcg gctgatttta aatcactacc gaatggtttt
     4381 tcgataacaa ggcgtttaaa tccttttgta tcagtaagac cagaagattt tagataatca
     4441 gaaataacgc caaagaattg tggtgccatt gctaaataga atagtcgatt accttttaat
     4501 tcaaattggc tatctaattc attactaaaa tctagtaatt cttgatagct ttcttcatta
     4561 ctaacatcat gtctatgata gaagacatgt tccataaacg cgtcaatttt gtttgtatct
     4621 tttacgtgct tttgaattga tgattttact tgattacgga aatcatcatt agtaatgtca
     4681 cgacgtccaa taccgatgat ggcaatatgt tcatctaaat tgtcttgttg gtagagatgg
     4741 aatattgatg gaaacaactt acgatggctt aagtcaccag ttgcaccaaa gattgtgatt
     4801 aaacatggga tgtgtttgtt tttagtactc aagattaaaa cctcaattct taattagata
     4861 tatgatttat tattataaga taatccattc agtaggtcat acaatatgct cgactgtatg
     4921 cagtgtctta aatgaaatat cgattcatgt atcatgttta atgtgata
//
LOCUS       DAKTIP010000178         4929 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAKTIP010000178 DAKTIP010000000
VERSION     DAKTIP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.181"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..950
                     /locus_tag="O0305_002296"
     CDS             <1..950
                     /locus_tag="O0305_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0275412.1"
                     /translation="FKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGYKPK
                     ELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVDVGF
                     LPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSLKDL
                     KGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDVDAA
                     AVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFIDIAK
                     SKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            1164..1937
                     /gene="phnC"
                     /locus_tag="O0305_002297"
     CDS             1164..1937
                     /gene="phnC"
                     /locus_tag="O0305_002297"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCY0275413.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            1939..2739
                     /gene="phnE"
                     /locus_tag="O0305_002298"
     CDS             1939..2739
                     /gene="phnE"
                     /locus_tag="O0305_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0275414.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            2736..3551
                     /gene="phnE"
                     /locus_tag="O0305_002299"
     CDS             2736..3551
                     /gene="phnE"
                     /locus_tag="O0305_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCY0275415.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(3682..4860)
                     /gene="deoB"
                     /locus_tag="O0305_002300"
     CDS             complement(3682..4860)
                     /gene="deoB"
                     /locus_tag="O0305_002300"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCY0275416.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
BASE COUNT         1627 a          843 c          854 g         1605 t
ORIGIN      
        1 attttaagtg tttatttgta ttaatgttag cagtcattgt ttttgcagca gcatgtggaa
       61 actcaagttc tttagataat caaaagaacg ctagtaatga ttcggattct aaatcaggag
      121 gatacaaacc taaagaatta accgttcaat ttgtaccttc gcaaaatgct ggaacattag
      181 aagctaaagc aaaaccatta gaaaaattac tatctaaaga attagggatt ccagttaaag
      241 tgtctgtatc aactaactac aatacaattg ttgaagctat gaagtctaaa aaagttgatg
      301 ttggtttctt accaccaacg gcatacacat tagcacatga tcaaaaagca gctgatttat
      361 tattacaagc acaacgtttc ggtgtaaaag aagatggttc agcaagtaaa gaacttgtag
      421 atagttataa atcagaaatt cttgttaaaa aagactcaaa aattaaaagc ttgaaagatt
      481 taaaaggtaa gaaaattgcc ttacaagatg taacatcaac tgctggatat acattcccac
      541 ttgcgatgtt aaaaaacgaa gcaggtatta atgcaactaa agatatgaaa attgtgaatg
      601 ttaaaggtca tgaccaagca gttatctcat tattaaatgg agatgtagat gctgcggctg
      661 tatttaacga tgcacgtaat actgtgaaaa aagaccaacc aaatgtattt aaagacacac
      721 gaattttaaa attaacacaa gctattccga atgacacaat ttctgtaaga ccagatatgg
      781 ataaagattt tcaagaaaaa ttgaaaaaag cttttataga cattgctaaa tcaaaagaag
      841 gtcacaaaat tattagcgaa gtttattcac atgaaggata cacagaaacg aaagattcaa
      901 atttcgacat tgtaagagag tacgaaaaat tagttaaaga tatgaaataa tcattattta
      961 acaaatgaat cattagcgaa tttggtatta aaagctttcg ttcaatagat atattctaga
     1021 ttaatattga aaagctaggc gctaaactga aacagatata gaaaggtgtc gctgtacatt
     1081 tgaaaccatt tgtacacaga aacccaatgt ctatgatatt tcagtttacc ttggcttttc
     1141 tttattaaag aaaggtgtca aacatgagtc aaatcgaatt taaaaacgtc agtaaagtct
     1201 atcctaacgg tcatgtaggc ttgaaaaata ttaacttaaa tattgaaaaa ggtgaatttg
     1261 cagttattgt cggactatct ggtgctggga aatccacgtt attaagatct gtaaatcgtt
     1321 tgcatgatat cacgtcaggt gaaattttca tccaaggtaa atcaatcact aaagcccatg
     1381 gtaaagcatt attagaaatg cgccgaaata taggtatgat tttccaacat tttaatttag
     1441 ttaaacggtc aagtgtatta cgaaatgtac taagtggacg tgtaggttat caccctactt
     1501 ggaaaatggt attaggttta ttcccaaaag aagacaaaat taaggcaatg gatgcactag
     1561 aacgcgtcaa tatcttagat aaatataatc aacgctctga tgaattatca ggtggccaac
     1621 aacaacgtat atctattgca cgtgcgctat gccaagaatc tgaaattatt cttgcagatg
     1681 aaccagttgc ttcattagac ccattaacta cgaaacaggt tatggatgat ttaagaaaaa
     1741 tcaaccaaga attaggcatc acaattttaa ttaatttaca ttttgttgac ttggcaaaag
     1801 aatatggcac acgcatcatt ggtttacgtg atggtgaagt tgtctatgat ggtcctgcat
     1861 ctgaagcaac agatgacgta tttagtgaaa tatatggacg tacaattaaa gaagatgaaa
     1921 agctaggagt gaactaacat gcctttagaa atacctacaa agtatgactc ccttttaaag
     1981 aaaaaggttt ctttaaaaac gagttttacc ttcatgttaa tcattgtgct tatcatttgg
     2041 agcttcattt atacgggatt taattttgga gatttaatga taggaatacc tcaaataggt
     2101 gatctattca aacaaatgat tccacctgat ttcgagtatt tacaacaaat tacaacgcca
     2161 atgttagata ccattcgaat ggctatcgta agtacagtat taggtagcat cgtttcaata
     2221 ccaattgcgt tattatgtgc tagcaatatc gttcatcaaa agtggatttc aataccctcg
     2281 cgctttattt taaatatagt tcgtactatt ccagatttgt tattagcagc aatctttgtg
     2341 gctgtatttg gaatcggtca aattccaggg atattagcac tgtttatttt aactatctgt
     2401 attattggaa aattattata tgaatcattg gaaacgatag atccaggtcc aatggaagca
     2461 atgacggctg ttggcgctaa taaaataaaa tggattgttt tcggtgttgt accacaagcc
     2521 atatcgtcat ttatgtcata cgtattatat gcatttgaag taaatatacg tgcttcagct
     2581 gtgcttggat tagtcggcgc tggcggtatt ggattgtttt atgatcaaac acttggttta
     2641 tttcaatatc caaaaacagc aacgattatt ttatttactt tagttatcgt cgtcgtcatt
     2701 gattacatca gtacgaaagt gagggcacat ctcgcatgac acaggaaata gcaaaatata
     2761 atgttcacac aaaagcacac aaacgaaaat tgattaaaag atggcttatt gcaattgtcg
     2821 tcttagctat tatcatctgg gcatttgcag gtgtaccaag tttagaactt aaaagtaaat
     2881 cattagaaat cttaaaatcc atattcagcg gattattcca tcctgatatc agctatatct
     2941 atataccaga tggcgaagac ttattacgtg gtttacttga aacctttgcg atagccgttg
     3001 taggtacttt catcgccgca attatctgta ttccattagc atttctaggt gcaaataata
     3061 tggtaaagct acgcccagtt tcaggtgtta gcaaatttat tttaagtgtt atacgtgtct
     3121 tcccagaaat tgtaatggca cttatattta tcaaagctgt tggcccaggt tcattttcag
     3181 gtgtattagc tttaggtatc cattccgtag gtatgcttgg gaaactttta gctgaagata
     3241 ttgaaggtct agatttcagt gctgtagaat cattaaaggc cagtggtgcg aataagatta
     3301 aaacactcgt atttgcagtc ataccacaaa ttatgcctgc ctttctatca ctcatacttt
     3361 atcgctttga actaaactta cgttcagctt ctatactggg gctaattggg gctggtggta
     3421 tcgggacacc actcatattt gccattcaaa cacgttcttg ggaccgtgta ggtattatat
     3481 taatcggttt agtactaatg gtcgcaattg tcgatttaat ttccggttca atccgaaaac
     3541 gtattgttta acattaaatc aggatactcc taaataagaa gtcctaccgt cttacgtttc
     3601 tctattataa taaaaacagc agtgaagaaa actattgtta tagttaactt cactgctgtt
     3661 tttataatat ctaaatttat tctatttcaa ttcctttaaa taacttttac cgaactctgg
     3721 taatgttacg ttgaaattat ctgctatagt tgcaccgata gaactgaatg tagtatcact
     3781 ttctagtgca tgaccacctt taaatttcgg actgtacata attactggga tatattctct
     3841 cgtatggtcc gtacctggcg ctgtcgggtc attaccatgg tctgctgtaa taattactaa
     3901 atcgtcttct tttaagttgc taaacagttc tggcaagcga tcatcgaaat ctttaattgc
     3961 ttgtgcataa cctggtttat cacgacgatg accgtataat gcatcaaagt ctactaagtt
     4021 taagaagcta atacctgtga aatctttctt aacaattttc atcaattgat ccataccgtc
     4081 catgttactc ttcgtacgaa ccgcttctgt tacaccttca ccatcataaa tgtcattaat
     4141 tttaccgatg gcaataacat cataaccacc gtctttcaaa tgatctaaga cagttttacc
     4201 aaaaggtttt aacgcatagt catgtcgatt agatgtacgt gtaaagtttc ctggttcacc
     4261 aacatatgga cgtgcgataa tacgaccaat taaatattta gggtcttttg tcaactcacg
     4321 aaccttttca caaatatcat ataactcttc taatgggata atgtcttcat gtgcagcaat
     4381 ttgcaatact gggtctgcac ttgtataaac aattaagtca ccagttttca tttggtgctc
     4441 gccccactca tcgataattt gcgtacccga tgccggtttg ttagcaacaa ctttacgacc
     4501 tgtcatttct tcaatttgtt gaattaactc ttcagggaat ccattagggt atactttaaa
     4561 aggttgcata atatttaatc ccataatttc ccagtgacca gtcattgtat ctttaccaac
     4621 tgaagcttca ctcaatttag tatagtatgc ttctggttgt tcaactgcat ttactactgg
     4681 taatttatcg atgttcccta gacctaactt ttcaaggttt ggtaaagttt gatcgaaacc
     4741 ttctaaggta tgtcttaaag tatgtgaacc ttcatcttta aaatcagctg cgtctggcgc
     4801 ttcaccaata cctactgaat ccattacgat taaatgtaca cgattaaatg gtcttgtcat
     4861 agctatcact cccaaaattt atatatatta gtaatctgaa tctgcttcta aaccttgcat
     4921 aatttgaac
//
LOCUS       DAKTIP010000179         4920 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAKTIP010000179 DAKTIP010000000
VERSION     DAKTIP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.182"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..462)
                     /locus_tag="O0305_002301"
     CDS             complement(<1..462)
                     /locus_tag="O0305_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCY0275417.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYL"
     gene            complement(1195..1560)
                     /locus_tag="O0305_002302"
     CDS             complement(1195..1560)
                     /locus_tag="O0305_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCY0275418.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            complement(1702..2124)
                     /locus_tag="O0305_002303"
     CDS             complement(1702..2124)
                     /locus_tag="O0305_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCY0275419.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            2258..2998
                     /locus_tag="O0305_002304"
     CDS             2258..2998
                     /locus_tag="O0305_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275420.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDETMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            2991..4214
                     /locus_tag="O0305_002305"
     CDS             2991..4214
                     /locus_tag="O0305_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275421.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(4338..4841)
                     /gene="traP"
                     /locus_tag="O0305_002306"
     CDS             complement(4338..4841)
                     /gene="traP"
                     /locus_tag="O0305_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCY0275422.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
BASE COUNT         1685 a          705 c          815 g         1715 t
ORIGIN      
        1 taaatataat aaatctgtaa tggaaatacc gaaattcaat gccaataaga acataaaata
       61 atgtccatat tgcgggaatg aaattgttag ataaacgaac attgcagata agataattaa
      121 cggcaatatt aagttaatgc aaaaataaaa tttgtgtaca ggttgattga cgtaggtatt
      181 atagaatata agccattttc ttttagttaa cttatgaact ttaaacgatt ttctatatgg
      241 taagaaaaat aataaatgta tacttttatg taatggatac gtcataaata caagtaataa
      301 aaagatgaga aaatgtctat ctgataatgg cgtattagat aaaaaataca ttacttcata
      361 actaactaaa aacataatga tggttgcaac tgcactcata aatgcaattc taggcaaacc
      421 aaatcgtgca ttgatatcaa tttgtctttt acataaaaac atatcttacg cctcatctct
      481 tttcaatata agttagatta taactaactc aggttcagta attcaatagt aacatatcat
      541 gttagaagaa ttgttatata agaagaaagg agcgacgtat tgtttgattt gaaacgcgaa
      601 agtggggatt ggtaaaattg tctgattgac tagcgtagaa gtagaatgca gaacaaattt
      661 ctgcgaacac atattcttgc taataaagca tcacttatga aaaaggcgcg tgtgacatga
      721 gtatttgaaa acttgaataa tataaaaaat ggatatggca gggcatatgc aaaagcatct
      781 tttgaccaac taagttatac aaatgctaaa aatatagtga tatgtaatta taataaacac
      841 cagattaata atttgtgcaa gtaaggtata tgtaatgtgt atataaggtt cggctcaatg
      901 aaaatataag tatgttgttt gtcgataatg atttaagtaa agcgtaatca aagtgcaagg
      961 ataacacaat gaatcctaag caatgttttc aatcaattca taaatatgat ttaagcgaaa
     1021 atcactacaa acaatgtctt cctataattt gtaaacataa gttcatttga gttttaaaaa
     1081 agagatgaca agaaatatct aatttctaat gtcatctcgt ttatttggca tttaatttta
     1141 aaaaataaat atgctgttac taataaaaat agttgtgatc gagaacgtaa cctgctactt
     1201 agaaatttcg tttccaatat cctcgccacg attttgtaaa ttttcgatgt gtgactgtaa
     1261 acgttcaata ttaggattaa tgtcagattt gaagtttcca agtaatgact taatttcacc
     1321 accaattgaa ctaccaaatt ccgcactttc gtttttaatt tgaacaccat agtttaaaat
     1381 gtcattaaaa ctttgtttaa tcgtttcaaa ttcacgttgt agttctgatt ttgaaccagt
     1441 aggggcagta cgatcgatcg tgttgttctt gacacttttg tcgtcacgtc cttgaagtgc
     1501 aactacaaaa ccagctgcta cgccaacacc gataccgaat agaatgcgtg atgctttcat
     1561 ttatattctc ctcactttta acatctgtta tcttgataac tcattttgtt taaaattgct
     1621 aaccttgatg ttatcacttc aaacgcaatt gttataatat taatcatatt atacccgttc
     1681 gagcttagat taagcataca tttaaaattg tgcttgaatt tgttcagcaa tttgttgttt
     1741 tgcatcgtta tctaaaatgt cttcatgtgt ttcccactta taaccaaatc catcaatatc
     1801 attttcgtat cgaggaatta agtggaaatg aatatgaaat acagattgat ctgcaaactc
     1861 accattattt tgaataatgt ttaaaccatc aggattaaat gcacgcttaa tagcatttgc
     1921 tactttaggt aatgctgcac cgatatgttt cattgtttct tcatcagttt caaagatatt
     1981 agcagaagct tttttaggaa ttaataacgt atgtccttta gtaacttgtg atatatctaa
     2041 aaaggcatag acataatcgt cttcatatac tttaaagcta ggaatttctc cagttaaaat
     2101 tttgccgaaa attgtttctg acatgatgac tacactcctt ttcaactagt attaccattc
     2161 attataacat tgtcgctaaa aattcgatat taggacatgt gtatttaata tgagcatttt
     2221 ataatttttg gtacaatata agtcatggag gtgccttatg acagttaaag tagaacagct
     2281 tacaggtgga tatggaaaac gccctgtaat taaagatata aattttgaat taaacaaagg
     2341 cgaaattgtt ggacttattg gccttaatgg cgctggtaag agtacaacga ttaaacatat
     2401 gctaggattg cttacaccta tggaaggatc tctatcaatt tcagatatca acattaatga
     2461 tgacatagag gcttatagaa gaaagttatc ttatattccg gaatcaccgg ttatttatga
     2521 agaactcaca ttagaggaac acattgagat gacagcaatg gcatatgata ttgatcgtga
     2581 tgaaacgatg aatcgagcaa tgccattatt aaagacattc cgtttagaaa atgagttgaa
     2641 agtttttccg agtcattttt ctaaggggat gaaacaaaaa gtcatgatta tttgtgcgtt
     2701 tatcgttaat cccgaattgt atattataga cgaaccattc cttggcctag atccattagg
     2761 aattcaatcc atgttagatt taatggttga aaagaaaaac gaaggtagaa ccgttctaat
     2821 gagtacacat attttagcaa cagctgaacg ttactgtgat cgttttatca tactagacga
     2881 aggtgaagtc gttgcatttg gagatttaga agcattgaga caacaaactg gcttacataa
     2941 tcaaacatta gacgatatat atattcatgt gacgcaaggt ggagatgtac atgcgtaacc
     3001 aagctaccac attatttaat aaaagattac atgcccttcg taaagagaaa aattattata
     3061 ataagtttat atttaatggt catttcatgg tctttttact tattttgctg ggtgcattta
     3121 tttttggata tggtgaatgg ttaaaacata ttccaacgaa tatcaatttt tcattaatag
     3181 cagcagtgat tgttgcgttg acatctattt ttccaatgcg accactgtta aaagaagcgg
     3241 ataaaatctt tttattacca tttgaaaaac acatgtctca atttatgcgt catgcgattt
     3301 tatatagtta ctttgcgcgc atattaatcc aactcattat tgttattgta atgttcccgt
     3361 tgttttacaa tattaatcaa cataatgtgg cattttatat ttgttttgga gtcagtgcat
     3421 taatttttcc atatgttggt ttacgcttaa gatggcaatg gtatcaatcg ggacttaaaa
     3481 catggcaagt taatttaata tcattcatca cctttgctct aacatattac ttactattag
     3541 ctccaaaatg gtacatcgca tttgtgatgg ttgctttgcc tgtgttaata gaatttttag
     3601 ttaaaaaata taaaccaggc tttttgtatc cttgggagaa aatgattgca atcgaacatc
     3661 gacatcatat gaattattat aaatttgtta atatgttcac cgatgtaaaa catttgaaag
     3721 aatctgcagt cagaagaagt tatctagata tacttttacc agttccaaag ggtagtaaat
     3781 ttaactctaa tgcgatgtat ttattcttat ttattagaag ctttataaga gggagagacg
     3841 catttaatat tatctttagg ttagtaatta ttgcagtttt attaatggtt tggttatcat
     3901 atccattagt gacagcaatt attgggtgtt tatttgtata tattatattg ttacaaatgg
     3961 cacaatttta ttcacagcaa gcatatggct tatggcctca agtgtggcct gtacctgaag
     4021 agaaggtaat caaagggtat gaacagtttt tatatagatt aatgtttgtt atttgtactg
     4081 tgtttgcagt gacatttatt ataaaacata tgacgttatt ctatgttgta ctcattttct
     4141 atattgtagg tttattaacg attagaagta ttattaaaaa gttgaaatat caggaaacat
     4201 tattacgaga ctaaaaaact tccattggca tgtatgttgt aaaggtgcat gtaatgttga
     4261 acgccaaatg atacggcgtt cagattacat tagcatctat acgttaacag cataaccaat
     4321 ggaagttttc ttcgaatcta ttcttttatt gggtatagat atctttcaaa ataacttgaa
     4381 tgttgtccgc ttgaaccaaa gtaatgactt aatgcgtctt ttgaaaaatg gtcattaaag
     4441 gcatcagatt gcttaaagtc ttcgtatgca tgtcgatcag caaatccgaa ataaattttg
     4501 tatgttgtac ctttagcagg tcttaacaaa cgatagcttt taaagccacc aaagtttctg
     4561 aaattatcgt ctacactaat cagtttcttt tcaagttgat atgcatgatc ttctgttgat
     4621 ggaatgaaga ttgcacaata gaaatgatgt tcactgaatt caccaatttc tttaataact
     4681 tcatatattg aaggtgattt taaaacagtc tcaccatcag tttcttcaaa aataactgaa
     4741 gtatctgatg ctgaaaattg gaatagttga tgggtcgggt tattgatttt tatctgatgt
     4801 aaaaatccat aagtgccata agatgtatat agtttcttca tatgaaaggt cccccttatg
     4861 tttttattta ttatacgatg ataaactagt cattaccact attaataatt gattaaaatt
//
LOCUS       DAKTIP010000180         4892 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAKTIP010000180 DAKTIP010000000
VERSION     DAKTIP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.183"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(210..1403)
                     /gene="sqr"
                     /locus_tag="O0305_002307"
                     /pseudo
     CDS             complement(210..1403)
                     /gene="sqr"
                     /locus_tag="O0305_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
     gene            complement(1422..2756)
                     /gene="cstB"
                     /locus_tag="O0305_002308"
     CDS             complement(1422..2756)
                     /gene="cstB"
                     /locus_tag="O0305_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCY0275423.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSVHSADMTGKEEHVLDVRNDEEWNNGHLDQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(2787..3854)
                     /gene="cstA"
                     /locus_tag="O0305_002309"
     CDS             complement(2787..3854)
                     /gene="cstA"
                     /locus_tag="O0305_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCY0275424.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENNNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            3991..4251
                     /gene="cstR"
                     /locus_tag="O0305_002310"
     CDS             3991..4251
                     /gene="cstR"
                     /locus_tag="O0305_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCY0275425.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            4251..>4892
                     /locus_tag="O0305_002311"
     CDS             4251..>4892
                     /locus_tag="O0305_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCY0275426.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDATFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIG"
BASE COUNT         1498 a          877 c          694 g         1823 t
ORIGIN      
        1 aaatatctta ctgctgtttc tattttcata atcattctta ttgaatgact tcatcgtgat
       61 catttaaaac agtaacaact ttttcaatct tatactattt agagctaaaa attcacgaca
      121 tataaacaca acactgacac gtcatttaaa tacttaatct tttatagtaa aagatttttc
      181 attaaaaatt tattgttttc tgtactttat tatattaatc cttttagcat gccgtaccaa
      241 tacattttag gtaataaatc tttcttaaat atatacatac ttctacgttc tttggcctga
      301 ttaaacggca ttgtttcttt agtattttta ttataatcaa actctgcaag tattaaccta
      361 ttatatccag taacaatagg gcatgaagta taaccatcat aatgatgcgt taacatttga
      421 ttattcatca cttgcaataa attattagcg acgataggtg cttgcttacg aatagctgcg
      481 cctgtttttg aagtaggtac atttgaagca tcaccaagtg caaatacatt agagtagctt
      541 ttatgctgta atgtggttgg gttaacatct acccaaccct cactatctga aagtgtactt
      601 tcttttacta catctaaggg acccataggt ggtgttacat gtaacatatc ataacttatt
      661 gtttttctat cgtatgcttt gatatgttcg aatgtagcca cttttttgtc accgtcgatt
      721 tcaacaaggt tataattata attgactgtt atatttcttt cttcaacaat cctttctaat
      781 tctttattat attttcctac gtcaaataaa gcatcttttg gcgttgcata tatcacatta
      841 gcgttagaac ggattttatg tttcctaaaa taatcttcag ctaaatacat aattttcata
      901 ggcgcacctc cacactttat aggagtgttt ggatgcgtaa aaatggcatt tccttgttta
      961 aaattagaaa tttggttcca agtttcgtta acatagtcag gtgaatagtt agagcaaaca
     1021 ccatttttac ctatattttc ttttagtcct ttaattgaag accaattaat ctgtaatcct
     1081 ggagctacta ctaaaaaatc ataataaacg actgtattat ctcctaaaat aacgctatta
     1141 ttttcaggtt aaaaacttga aacagcctgt tttatccagt tagcaccttc aggtataaca
     1201 ctttccatat ctttacgaga acttttcaaa ctagataccc ctgcaccaac caacgtccat
     1261 aatggttgat agtaatgatg gtctgctgga tctataattg ctattttctc ttttaagttt
     1321 tgattttttc ttaatagtct tgatgctacg gtaacacctg ctgtaccgcc accaataata
     1381 acaatttgat aatgcttatt cattttttcc acatccttaa attattttaa tgattctgga
     1441 aaatcttgat agccttctct aatattcacc acattttcaa aacctttgct ttccaatata
     1501 cccactgcaa ttgaacttct aacacctgac tgacaatgta catatatttt atcctcttta
     1561 ttaaaaggaa tattttcatt taataattta ccatgcggaa tattaactgc ttgatctaag
     1621 tgtccattat tccactcttc atcattacgt acgtctaata catgttcttc tttacctgtc
     1681 atatcagcgc tatgaacgga ctgggttgaa atgcctgatt ttggcaaacg atagcctgct
     1741 accttatcaa acccaattaa ttgtaaagtg tgtttcgctt tctcaacagt agatttatct
     1801 ccaattaaat ctatatcttt ttcaaaatct aagtaccaac caatttgatt aataaagttt
     1861 ttgttgtatg gtatattgat tgttcctttt gtgtggccac cgtgaaaggc ctctttgcta
     1921 cgaagatcaa atgctactct cttattatct aaactaggat aaacatcata tgattgatat
     1981 aaattcatac caaactgatt aacttgtttc atttgtgcaa aatgatgcgg tggtgctggt
     2041 tgatttgatg ttaatgattc aataaattta gtctcatcaa tttcattaaa tgcccagtta
     2101 ttaattttct cataacctat tgtagatata ggtatggcac ctaatgcttt accacaaggg
     2161 cttccagcac catgacccgg ccagatttga acatagtctg gtaaattttt aatattttga
     2221 acggactcat acatttgttt cgcgctaatt tctgtagaac cctttatttg aacagatttt
     2281 tctaataaat caggtctacc tatatcacca acaaaaataa agtcaccact aaataatccc
     2341 atcggaacac ttgaaccacc acctaaatca gtgagtaaaa agctaatact ttcaggcgtg
     2401 tgtcctggag tatgcagaac ttctaattta acattgccta cttgaatgat atctccatgt
     2461 ttaacaaatt gtgtttttga tggcatattt ttatacccta atgcatcttc accttcgcca
     2521 gacacatata tatttgcatt taagcgttta gccacatcac gaattcctga agcaaaatca
     2581 gcatgaatat gtgtttctgt agcttgtgta attgttaaac cttcagaatc tgcaacttct
     2641 atatatttcg ataaatcacg aacagggtct attattattg cctctcctgt acgttgacaa
     2701 cccactaaat atgatgcttg agataaatga ttatcgtaaa actgtttaaa aaacatgata
     2761 acaactcctt ttattaatag atttaattag ataaataaat tatgattcgc attttcagta
     2821 gcaccaatat aagtgcctac accaccgtac tcaacttcat ctctaagttc ttctttctga
     2881 atacccatga catccatact catcgtacaa gcgattaatt tgatattttg ctcaatagct
     2941 tgattgataa gtgttggtaa tgaatcaaca tttttctttt tcattacgta gcgcatcatc
     3001 atatttccta aaccaaacat attcattttg gaaagaggca ttcgtatatt ctttttgggc
     3061 aacattaaat caaacatttt tgcaatacct tgctttttaa cattaactgt ttgcactttt
     3121 tttaatgcat taagccccca aaaagtaaag aagatagtta catcttttcc agcagctcta
     3181 gcaccatttg caataatcaa cgctgctaca gctttgtcta attctccact aaataataca
     3241 attgtagtac ctttagcaga ataatttata tctaaatctt ttgctttttc tttttgaata
     3301 atcgcattaa ttccattgtt attttcatca agcttaacta aagtatgcct tgtttgtttc
     3361 acccaacttt taatgtcact agggaatcca gggtctgtga ctttgacttc aatttggtca
     3421 cctacttcaa tattcttcat ttcttgacta atttttacaa ttggccctgg acattgaaga
     3481 ccacgatagt taaattgttt acgatctgct ttaatttcta tacttttata ttcttcttgt
     3541 gtatcataac tatcgttttc ttcttcataa gctttataac caccatcaag atttataacg
     3601 ttataacctt gtttagctaa atatttacta gcattagcac ttctgttacc acttctacag
     3661 attacataat aggttttatt tttttcttta ttgaatgact caatctcatc aacaggatga
     3721 agtatggaac cattgatatg tcctaatgca tactcctctt ctgttctaac gtcaattaat
     3781 tgcccttgct tggctagttt ttccaattct gctttactaa attcatcgat aaacttttct
     3841 ccgtattgtt tcataataac ctccctaaaa atacctgtag gggtatttta aacagttgga
     3901 tggaacttgt caaatacctc taggggtatt tgacacattt gtgtttatct attaatatat
     3961 ttttattcaa ttaaaagtgg aggtatgact atgaattatg ataaaaaaat gattaatcgt
     4021 attaatagaa tacaagggca actaaatgga attattaaaa tgatggagga aggaaaagac
     4081 tgtaaagatg tcattacaca aataagtgca tcaaagagtt cactccaacg cttgatgggt
     4141 atcattatta gtgagaattt aatagaatgt gtaaaagcag ctgcggatga tgaagaaagc
     4201 tcccaagagt taattaatga agctgtaaac ttattggtga aaagtaagta atggatattg
     4261 ttaatatagt tatcatgtta ctcattgggg tattcggtgg gttcatatct ggtctagtag
     4321 gcgtaggtgg tgctatcatt atttatccag ctattttatt attaccacca ttattcggag
     4381 cccctgcata tagcgcatat atcgcatcag gtcttacttc tagccaagtc tttttcagta
     4441 cattaagtgg ctcattaaaa gctcgaaaaa aaacagagtt ctcgccacaa ttagttcttt
     4501 atatgggtgg cggcatgatt atagggagta tgttaggtgc atttctagca aatttatttg
     4561 atgctacatt tgtaaatacg gtatatatca ttattgcttt acttgcatta acattaatgt
     4621 ttattaaagt gaaacctagt tccgaaaagt cttcatttaa taaatattta ttagttatca
     4681 ttggcctttt cattggtatt atttcaggta tagtaggtgc aggtggtgcc ttcattatta
     4741 ttcctatatt attggtttta ttcaaattac caatgaatac tgtagtcgct aatagtatag
     4801 taattgcttt tatatcttca attggtgctt ttgtcatcaa acttattcaa ggttatatcc
     4861 ctttatatga cgccttattc cttataatag gg
//
LOCUS       DAKTIP010000181         4809 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.184, whole genome shotgun sequence.
ACCESSION   DAKTIP010000181 DAKTIP010000000
VERSION     DAKTIP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.184"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(133..1101)
                     /locus_tag="O0305_002312"
     CDS             complement(133..1101)
                     /locus_tag="O0305_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCY0275427.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            1296..1751
                     /locus_tag="O0305_002313"
     CDS             1296..1751
                     /locus_tag="O0305_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0275428.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            1991..3373
                     /locus_tag="O0305_002314"
     CDS             1991..3373
                     /locus_tag="O0305_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275429.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANISVTLKGDTKLNDSSK
                     KVTINDHEMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            3404..3532
                     /locus_tag="O0305_002315"
     CDS             3404..3532
                     /locus_tag="O0305_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275430.1"
                     /translation="MEYKQHPFKVFTISVNYFEGIVFCGKIYEVLRFISFYTFYKL"
     gene            3642..>4809
                     /locus_tag="O0305_002316"
     CDS             3642..>4809
                     /locus_tag="O0305_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCY0275431.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILII"
BASE COUNT         1788 a          613 c          858 g         1550 t
ORIGIN      
        1 agggattaat gtcccagact ctttagttta tttattttca atataacaat tgtctaatca
       61 aggattaacg aatatttaaa gatagtttga cgcaatatta gaaacaacct ataataatag
      121 tttgtttgtg gattaactat tataaataaa agcggcgtaa agacatataa accaactact
      181 tgaacaatat aacgttaata acaatctata ctgatacatt acgcctagat aatctttgat
      241 gagcacatgt aagaaaaagt gatatggtgt atgacttccg acaccatcga tagataaacc
      301 taatttttgg gctagtcgta aggcgcgcaa tacatgaaac tgacttgtta cacaaacaat
      361 tttaactgct tcatgataca aattgttgat gatttgttta gaatataaaa agtttgtgta
      421 tgtatttata gagtgagatt ccattagtat atctgtttta tcaacaccat gtgcaatcaa
      481 ataacgttgc atagctaaag cttcagaaat tggttcgtct ggtccttgtc cgccagatac
      541 aatgatcttt gttgctgatg cttgttgttg atagatatca agtgcacgat ctaaacgcgc
      601 tgcaagcatt ggtgtgacaa attcggtaaa aataccagca cctaacacaa ttatgatatc
      661 aacttctttg ttgtatgatc tatgtctata tgatactgtc caaacgagat aacaaataaa
      721 ggttagtaac agggaaagac ataatatagc taaccacata gacaaacctt tcacaatagg
      781 tgactgaatc gtacttataa atagaagtgc tgatgtgtag agtacaaatt tatatgaaaa
      841 agataataat tttttaataa ataagcgact agaagtatga gaaaataaat atctatgttt
      901 gaatagcatg ataatactga ttattataaa tgttacaaac atagaccaag ggaaagtata
      961 ggtcatgatg ctatagatga gtgacaaaaa tatcgatatg acaactaaga tgtagcatgt
     1021 taaatttaac gtcagagtat agttgaaaat taacggacaa ataacgataa gtataaatat
     1081 taataataaa ttcaataaca tactgacacc tcgcttataa taaatattaa atataaatgt
     1141 agatgattta atttattaaa gcaaggagaa agcagcaaca tgtaaatctt aatttgttat
     1201 attatatatg ggtcaatatt tttgtgtttt ttagatgtgc gatacaatga ttgctgagga
     1261 tactttataa aaatagtaat tagggagaaa taattatggt aaaacattta caagaccata
     1321 ttcaattttt agagcagttt ataaataacg ttaacgcatt aactgcaaaa atgttgaaag
     1381 atttacaaaa tgaatatgaa atttcattag agcagtctaa cgtattaggt atgttaaata
     1441 aagaaccttt gacaattagt gaaatcacgc aaagacaagg tgtaaataag gccgcagtaa
     1501 gccgacgaat taaaaagtta atcgatgcta aattagttaa gttagataaa ccaaatttaa
     1561 atattgatca acgtttgaaa ttcataacct taactgacaa aggtagagca tatttgaaag
     1621 aacgtaatgc gattatgaca gatattgcgc aagatattac taatgattta aattctgaag
     1681 atattgaaaa tgtaaggcaa gtattagaag tcatcaatca tcgcattaaa acatatagca
     1741 atcataaata ggtttttgaa ggtgttcaaa tataagacta aatatgtatg tattgaacac
     1801 cttcttttta gttataattg agagagtact ttcataaata atgtaataag gtacaattct
     1861 aaagtaaagt taatcatttc ttgaattgta ttcttgggat aatatttgta aaataataat
     1921 ggtaaaataa tgaaataatt tttgtgatat acatataaca attgaagagg aacagaattg
     1981 aggtgaaagt atgaaatctt gcccgaagtg cggacaacaa gcacaagatg atgtacagat
     2041 atgtacacaa tgtggacata aatttgatag tcgtcaagca ttatatagaa agtcaaccga
     2101 tgaagatata caaactaata acatcaagat gagaaaaatg gtgccttggg caattggttt
     2161 tttcatttta atcttaatta ttatattgtt ctttttacta agaaacttca attcacctga
     2221 agcgcaaact aaaatattag tgaatgcgat tgagaataat gataaacaaa aagtagcaac
     2281 attattaagt actaaagata ataaagtaga ttctgaagaa gcaaaagtat acattaacta
     2341 tatcaaagat gaagttgggc ttaagcaatt tgtcagcgac cttaaaaata cggtacataa
     2401 attgaataag agtaagacca gcgtagcttc ttatattcaa accagatctg gtcaaaatat
     2461 attacgtgta agtaaaaatg gcacacgtta tatctttttc gataatatga gctttactgc
     2521 acctaccaag caaccaattg ttaaaccgaa agaaaaaaca aaatatgagt ttaaatctgg
     2581 tggtaagaaa aagatggtta tagctgaagc aaataaagtg acgccaatag gtaattttat
     2641 accggggaca tatagaattc cagctatgaa atcaactgag aacggtgatt ttgcaggcca
     2701 tttaaaattt gattttagac aaagtaattc tgaaacggta gatgttactg aagattttga
     2761 agaagcaaat atatctgtaa ctttaaaagg cgatacaaaa ttaaatgata gttctaaaaa
     2821 agtaactata aatgaccatg aaatggcatt ttcaagttcc aaaacgtatg gtccatatcc
     2881 acaaaataaa gatattacca tttcagcttc aggtaaagcg aaagataaaa catttacaac
     2941 acagacgaag acgattaaag ctagcgattt aaaatacaat acagagataa ctttgaattt
     3001 tgacagtgaa gatatcgaag actatgttga aaagaaagaa aaagaagaaa acagcttgaa
     3061 gaacaaattg atagaattct ttgctggata ttctttagcg aataatgctg cgtttaatca
     3121 gtcggatttt gattttgtat catcatatat aaaaaaagga tcatcttttt atgatgatgt
     3181 aaagaagcgt gtatctaaag gaagtttaat gatgattagt tcaccacaaa ttatagatgc
     3241 tgaaaaacat ggtgataaga ttaccgcaac cgtaagatta ataaatgaaa acggtaagca
     3301 agtagataaa gaatatgagc ttgaacaagg ctcgcaagac cgcttgcaat taatcaagac
     3361 atcagaaaaa tagagtataa aacttaaata agatgcatgt gtcatggaat acaaacaaca
     3421 tcctttcaaa gtatttacaa ttagtgtgaa ttactttgaa gggattgttt tttgtggaaa
     3481 aatttatgaa gtgctgaggt ttatatcatt ttataccttt tataagttgt aatgatgttc
     3541 atgaaacttg tgactaatgc taatatataa aagagcatat ttaaatttag tttctgttcg
     3601 tgatagttat aaactaatca ttatataaag gagatagtca tatgaatgat ttaacgcatt
     3661 ttaacaagcg ttcaccgata tttattatta ttttaggatc tttgacggcc attggagcat
     3721 tatcgataga tatgtttttg cctggattac cagatattag acatgatttt caaacaacga
     3781 cgtctaacgc acaattaaca ttatcgatgt ttatgattgg tttagcgttt ggtaatttgt
     3841 ttgctggtcc aatttcagat tcaactgggc gcagaaagcc gcttattata gctatgatta
     3901 tttttacatt agcaagttta ggtattgttt ttgtacataa tatttggctg atggttgcat
     3961 taagatttct tcaaggagtg acaggtggcg cagcagcagt tatttcaaga gctatagcaa
     4021 gtgatatgta tagtggtaat gaactaacta agtttatggc attattgatg cttgttaatg
     4081 gtattgcacc agttgttgcg ccaacaatag ggggcataat tttaaattat tctgtctggc
     4141 gtatggtctt tgtcattctg actatctttg gcttcgtcat ggtcataggt tctttattaa
     4201 aagtacctga atcattaaca gtgacaaatc gtgagtcaag tagtggatta aagacaatgt
     4261 ttaaaaactt caaaatatta ttgaagacgc cacgttttgt attaccaatg ctcattcaag
     4321 gtatgacatt tgtaatatta tttacttata tttctgcgtc tccgtttatt attcaaaaaa
     4381 tttatggcat gactgcaata caatttagtt ggatgtttgc aggaattggt attacgctca
     4441 ttatatcaag tcaacttact gggtaccttg ttgattttat agactcacaa aaattgatga
     4501 gaggcatgac gatgattcag attatcggtg taattttagt gacgattgta ttgcttaatc
     4561 attggaactt ttggatttta gcgataggat ttatcatttt aatagcacca gtgacaggtg
     4621 ttgcgacact tggttttaca atagctatgg atgaaagtag tagtggtaga gggagttcat
     4681 caagtttatt aggtttagta cagtttttat ttggtggtgt tgcatcgcca ttagtggggg
     4741 taaaaggtga agataatcct ataccttaca taattattat tatagctaca gcagttatat
     4801 taattattt
//
LOCUS       DAKTIP010000182         4797 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAKTIP010000182 DAKTIP010000000
VERSION     DAKTIP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.185"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            152..2941
                     /locus_tag="O0305_002317"
     CDS             152..2941
                     /locus_tag="O0305_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCY0275432.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAPLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTIAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            3146..3988
                     /locus_tag="O0305_002318"
     CDS             3146..3988
                     /locus_tag="O0305_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCY0275433.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTILNKLLLGRHSINTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNDKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPAEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            3990..>4797
                     /locus_tag="O0305_002319"
     CDS             3990..>4797
                     /locus_tag="O0305_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCY0275434.1"
                     /translation="MYYPYLRGKQNELFAIRELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVAHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFVPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQF"
BASE COUNT         1823 a          616 c          894 g         1464 t
ORIGIN      
        1 attttagtat acgacttacc tcaaagacaa atattaaaag ataagacaat aaaattagaa
       61 taaatttacg tttgaagata aaaaacaaac atttttcatt aaagtttatg atatatttag
      121 agcaatagaa agtgtatgga aggggatatg aatggcatac caaagtgaat acgcattaga
      181 aaatgaaatg atgaatcaac ttgaacaatt gggttacgaa agagtaacga tacgtgataa
      241 taagcaattg cttgataatt ttagaacgat tttaaatgag cgtcatgcgg acaaattaga
      301 aggcaatccc ttaacagata aagaatttca acgtctgtta acgatgattg atggaaaaag
      361 tattttcgag agtgcccgta ttttacgtga taaattacca cttagacgtg atgatgagtc
      421 tgagatttat ttgtcgtttt tagatacgaa aagttggtgt aaaaataagt ttcaagtgac
      481 gaatcaagta tctgtcgagg atacatataa agcacgttat gatgtaacga tattaatcaa
      541 cggactaccc cttgtccaag ttgaattgaa acgtcgaggt attgatatta atgaggcgtt
      601 taaccaagta aaacgttacc gcaaacaaaa ttacacaggc ttattccgct acatacaaat
      661 gtttatcatt agtaatggtg ttgaaacgcg atacttttct aataatgata gcgaactatt
      721 gaagagtcac atgttttatt ggagtgataa acagaataac cgtatcaata cattgcaatc
      781 gtttgctgag tcatttatga gaccttgtca attagctaag atgatatcgc gctatatgat
      841 tattaatgaa acagatagaa tactgatggc aatgcgtccg tatcaagtgt atgcggtaga
      901 agcacttatt caacaagcga ctgagacagg gaataatgga tatgtatggc atacaactgg
      961 aagtggtaag acgttgactt cttttaaagc gagtcagatt ttatcacagc aagatgacat
     1021 taagaaagtt atctttttgg ttgaccgtaa agacttggat agtcaaacag aagaggaatt
     1081 taataaattt gctaagggtg ctgtagacaa aacttttaat acctcgcaac tggtacgcca
     1141 actaaatgat aaaagtttgc cacttattgt aacgacgatt caaaaaatgg ctaaagcgat
     1201 tcaagggaat gcccctttat tagaacagta taaaacgaat aaagttgtat ttattattga
     1261 tgagtgtcat cgcagtcaat ttggtgacat gcatcgtcta gttaaacaac atttcaaaaa
     1321 tgcccaatac tttggattca ctggtacgcc acgttttcca gaaaatagta gtcaagatgg
     1381 tagaacaact gcagatattt tcggtagatg cttacatacg tatttaatta gagatgccat
     1441 tcatgatggt aatgtacttg gtttctcagt tgactatatt aatactttta aaaataaagc
     1501 tttaaaagca gaagataaca gcatggttga agcaattgat acggaagaag tatggttagc
     1561 ggataaacgt gtggaattag taacacgaca tatcatcaat aatcatgata aatatacacg
     1621 taatcgtcaa tattcaagta tatttacagt ccaaagtatt cacgcgctta ttaaatatta
     1681 tgagacattt aagcgactta acaaaaagtt ggaacaaccg ttaacgatag ctggtatatt
     1741 tacgtttaaa cctaatgaag atgatcgtga tggtgaagtg ccatatcatt cacgtgaaaa
     1801 attagagata atgattagtg attataataa aaagttcgag acgaattttt caacagacac
     1861 aactaatgag tattttaatc atatttcaaa aaacgttaaa aagggcgtta aagatagtaa
     1921 aattgatatc ttaatcgttg ttaatatgtt cttaactggt tttgatagta aagtactgaa
     1981 cactttatat gttgataaga atttaatgta tcatgattta attcaagcgt attcacgtac
     2041 aaatagggtt gaaaaagaat caaagccatt tggtaaaatt gtaaactatc gtgacttgaa
     2101 aaaagagaca gacgatgcac tgagagtatt ctcacaaaca aatgatacgg atacaatttt
     2161 aatgcgcagt tatgaagagt ataaaaaaga atttatggac gcttatcgtg agcttaaaat
     2221 gattgtgccg acaccacaca tggttgatga cattcaagat gaagaagagc taaagcgctt
     2281 tgttgaagct tatcgtttat tagctaaaat aatattacgt ttaaaagcat ttgacgagtt
     2341 tgagtttaca attgatgaaa ttggaatgga tgaacaagag aatgaagact ataaaagtaa
     2401 atatttagct gtgtacgatc aagtaaaaag agcgacggct gagaaaaata aagtatccat
     2461 tttaaatgat attgatttcg aaatagaaat gatgcgtaat gatacgatta atgtgaatta
     2521 tattatgaat atattgagac aaattgatct tgaagacaaa gcggaacaac gtcgtaacca
     2581 agaacaaatt agacgcattt tagatcatgc agatgatccg acattgaggt taaaacgaga
     2641 tctaattaga gaattcatcg acaatgttgt accttcttta aataaggatg atgatatcga
     2701 tcaagaatat gttaatttcg aaagtattaa aaaagaagcg gagttcaaag gatttgctgg
     2761 agagagatct atcgatgaac aagccctaaa aacaatttca aatgactacc agtatagtgg
     2821 tgttgtaaac ccacatcacc ttaaaaaaat gattggtgat ttgccattga aagaaaagcg
     2881 taaagcaaga aaagccattg aatctttcgt ggcagaaaca actgaaaaat acggtgtgta
     2941 atgattcagc cccctcgcta gattagtgta gggggcatta ttattttcta tcttttgtta
     3001 gttaccatgt gtttggcaac catcatttgg tttcaataca attttataat cgtatgtgta
     3061 tagaaataat gtctataggc atttaaaata gtttataata ttaacaggat aagtattaaa
     3121 tgtatgttga aggaggtgcg tagctatgga taatagaaat atgattaatc gtgtttttag
     3181 tcaaaagata ttacatcaaa ttgcaatcaa aaataaaagt gatgttgttg atgaggcata
     3241 tgatttttat atacagggac ctaaaaatat caatgtaata cagaagatga aatctttata
     3301 taactatctt aaaaagtctt atcgtaacga atatttttac aaaaacacaa tacttaataa
     3361 actccttcta ggacgacatt ctattaatac aactactgca ctttctgaga tgcccatagg
     3421 gaaaagtatt gctgatttta tattgttaaa cggcaaaggc gttgtctatg agattaaaac
     3481 agaattagat aagctggata gattagataa tcaaattaat gattattatg aagtgtttaa
     3541 ttatgtcgta gttattacaa atgacaaaca tctgaataaa gttatggcta gatacaaaga
     3601 tacaacagtt ggaattttag tgttaacaac tagaaataca ctgagtgaag ttcaaaaacc
     3661 taaagaaaac aatagtctct taaacacaaa agcgatgtac aactttttac gaaaagaaga
     3721 aagaaaaaga gttattgcac aaaatcatat ggatgtgcca acttataatg atttcacaga
     3781 gtatgatgtg ttatttgacg tgtttaaaga aataccaatg acgaaactgc ataacaatat
     3841 gatttctgag ttgaaaaaaa gaggaaacat gaaagaatac aaagatgaat ttttagcagc
     3901 gccggctgaa attaagttct tgttatattt cgcaaaaatg acaaagaaag ataaaaataa
     3961 actatatcat tttcttaagg aggattaata tgtattatcc ttatttgcgt gggaaacaaa
     4021 atgaactatt tgcaattaga gaattgttag agaaaggttt gattggtgat tgtattcaac
     4081 ctataattga accaattaaa tatacaacca catttaaaaa tactttgcaa tactgtggtg
     4141 aaaaagcatt ctctataaat ttagtagtaa attcaaagtt aactgaagaa gagattagta
     4201 acgaaactgt tgcacattta actgaaataa taacaaaaaa caaaagtgtt attcaaaaag
     4261 cttacttggg tccttctgat gaaggcaatg ataggttgaa acagcaattt tcaagtaata
     4321 gtttagctat tttaacaagt gtagatgatt gggaaatgtt tggagataaa aataaacttg
     4381 aaatggtttt tgtaccagat gatagacaca ttaaacgtaa attgcgtaat attccaaaca
     4441 aaggcatcat tatggatcct tttaataaac taagtcgtaa tgttgattat ttagataatg
     4501 atgacgagtt ttatagcgac gatcaccttt attataagga agatggatac gtagcatttt
     4561 cagactattc tgttataggt ggagaatatg tagacggtgg cttttcgcca ttagctattg
     4621 cgatacatat tgtctatttt gatgaggcta atgagctaag agttaagcat tttgtctctg
     4681 attctaataa tgatagatca aatccaggta aaaagttttt tgaggctgta gataaattag
     4741 taacatggtc aaaaaactta gatattaaaa atagatctta tgcgcttgga caatttg
//
LOCUS       DAKTIP010000183         4793 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAKTIP010000183 DAKTIP010000000
VERSION     DAKTIP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.186"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1248
                     /locus_tag="O0305_002320"
     CDS             <1..1248
                     /locus_tag="O0305_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCY0275435.1"
                     /translation="GTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNI
                     SQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLK
                     LLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTK
                     EQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGIS
                     GVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVI
                     GDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDS
                     DAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLE
                     SSPEELITKHGYEVDKVYPVEGV"
     gene            1254..2315
                     /locus_tag="O0305_002321"
     CDS             1254..2315
                     /locus_tag="O0305_002321"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCY0275436.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2317..3231
                     /locus_tag="O0305_002322"
     CDS             2317..3231
                     /locus_tag="O0305_002322"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCY0275437.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            3289..4092
                     /locus_tag="O0305_002323"
     CDS             3289..4092
                     /locus_tag="O0305_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0275438.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(4385..4699)
                     /locus_tag="O0305_002324"
     CDS             complement(4385..4699)
                     /locus_tag="O0305_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275439.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(4674..4778)
                     /locus_tag="O0305_002325"
     CDS             complement(4674..4778)
                     /locus_tag="O0305_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275440.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
BASE COUNT         1724 a          655 c          936 g         1478 t
ORIGIN      
        1 ggtactgtcg gatctggtgt tgttaaaatc atcgaagaga accgacagca aattcaagat
       61 acattaaata aagatattgt cataaagcat attcttgttc gagataaatc taaaaagaga
      121 ccgctaaata ttagccaata tcatttaact gaagatgtta atgaaatttt aaatgatgat
      181 tcattagata ttatcgttga agtcatggga ggaattgaac caactgtaga ttggttaaga
      241 acagcactta aaaataaaaa acatgttatt accgcaaata aagatttatt agcagtacat
      301 cttaaacttt tagaagattt agcagaagaa aatggtgtag ctttaaagtt tgaagcgagt
      361 gtagcaggtg gtattccgat cgtaaatgcc ataaataatg gtttgaatgc gaataatatt
      421 tcaaaattta tgggaatttt aaatggtacc tctaatttta ttttatctaa aatgactaaa
      481 gagcaaacga catttgagga agcacttgat gaagcgaaaa gacttggttt tgctgaagcg
      541 gatccaactg atgatgtaga aggggtagat gcagcgcgta aagttgtcat tacatcatat
      601 ttatcattta accaagtcat taaattaaac gacgttaaac gaagaggaat tagtggcgta
      661 actttaactg atattaatgt agccgatcaa ctggggtata aaattaaatt gattggtaag
      721 ggaatatatg aaaatggcaa agttaatgca tcggtagaac caacgttaat tgataaaaag
      781 catcaattag cagctgtaga ggatgaatat aacgcgattt atgttattgg tgatgccgtt
      841 ggtgacacga tgttttatgg aaaaggagca ggcagtttag caacaggtag tgccgttgtc
      901 agtgatttat tgaatgtagc attattcttt gaatcagatt tacacacatt gccaccacat
      961 tttgaattaa agacagataa aacacgggaa atgatggatt cagatgcaga aattaatatt
     1021 aaagaaaaat ccaatttctt tgtagtagtg aatcatgtca aaggttcaat tgaaaatttt
     1081 gaaaatgagt taaaggcaat attaccattt caccgatcat taagagttgc aaattacgat
     1141 aatcaatcat atgccgctgt tatagttgga ttggaatcat caccggaaga attaatcact
     1201 aagcatggat acgaagttga caaagtatac ccagtagaag gagtttaatt ataatgagaa
     1261 gatggcaagg attagtagaa gagtttaaag cacatttacc agtaaatgaa aatacaccaa
     1321 aattaacatt gaacgaggga aatacaccac tcattcattg tgaaaatatg tctaaaatac
     1381 taggcataga tttatatgtg aagtatgaag gtgccaatcc gacaggttca tttaaagatc
     1441 gcggtatggt aatggctgtg acaaaagcaa aagagcaagg taagaaaatt gtaatatgcg
     1501 cttcgactgg aaatacatca gcgtctgcag cagcatatgc agcgagagca ggtttaaaag
     1561 ctatcgtcgt aataccagaa ggtaaaattg cattaggtaa attgtcgcaa gcagtaatgt
     1621 atggtgcaga aatcgtttct attgaaggaa actttgatga agctttagaa attgtaaaag
     1681 aaattgcaaa aagtggcgaa atcgagcttg taaactctgt caatccattt agaatcgaag
     1741 gacaaaagac aggctcattt gaaattgtac aacaattaga cggtgaagca cctgatattt
     1801 tagcgattcc tgtaggtaat gcaggtaata ttactgcata ttggaaaggc tttaaagaat
     1861 atcatgaagc taaaggatca caattgccga aaatgtttgg cttccaagct gaaggcgcat
     1921 caccaattgt tcaaaataaa gtcattaaaa atcctgaaac gattgcaact gctattcgaa
     1981 ttggtaatcc tgctagttgg gataaggcga ctaatgctct taaagaatca aatggattaa
     2041 tagatagtgt tactgatgat gaaattctag aagcatatca gttaatgaca actaaagaag
     2101 gtgtctttag tgaaccagcg agtaatgctt ctattgcagg tttaattaaa ttgcatagac
     2161 aaggtaaatt acctcaaggt aaaaaagtag ttgctatttt aactggtaat ggattaaaag
     2221 atcctgatac tgctatttca ctactagata atccgataaa gccattgcca aatgataaag
     2281 atagcattat cgattatatt aaaggagctt tataacatgt cgaatgtttt ggagttaaca
     2341 attcctgcat caacagccaa ccttggagtt ggctttgatt ctataggtat ggctttagat
     2401 aaatttttgc atctgtctgt aaaggaaaca tcagggacaa aatgggaata tattttccat
     2461 gatgatgcat ctaagcaatt gcctactgac gaaacaaact ttatttatca tgtagcacaa
     2521 caagttgctt ctaaatatag tgttgacttg cctaatttat gtatcgaaat gagaagtgat
     2581 attccattgg caagagggtt aggttcgtca gcttctgctt tagtaggagc tatatatatc
     2641 gcaaattatt ttggtgatat ccaactgtct aaacatgagg tattacaatt agcgactgaa
     2701 atcgaaggac atcctgataa tgttgcgccg accatttatg gtggtttaat cgctggatat
     2761 tataatgatg tctcgaaaga aacgtcagtt gcacatatcg acataccaga cgtggatgtg
     2821 attgtaacga taccaactta tgaactaaaa acagaagcat caagacgtgc tttaccacaa
     2881 aaattaacac atagtgaagc ggttaaaagt agtgcaatta gtaatacaat gatttgtgca
     2941 ttagcacagc acaattatga attagcaggt aaactcatgc aacaagatgg ctttcatgaa
     3001 ccgtatcgtc agcatttaat tgctgaattt gatgaagtga aaacaattgc tagtcaacat
     3061 aatgcctatg caactgtaat tagtggtgct ggaccaacta ttttaatatt tagtcgtaaa
     3121 gaaaatagtg gggaattggt tcgctcttta aatagtcagg tagtatcatg ccattctgaa
     3181 ttggtcgata ttaatatcag tggtgttaaa gaacgaattg tataccaata gacgctttat
     3241 attgtaaaat agtattaaat gcagaataga gaggagattt aatgcgatat gacaaattat
     3301 aaagttgtcg ttttagatat ggatgacaca ttgctaaatt cagataatgt gatatcagaa
     3361 gaaactgcaa attatttaac agcaattcaa gatgaaggtt attatgttgt tctagcatct
     3421 ggtagaccta ctgaaggtat gattccaact gctagagatt taaaattacc tgaacatcat
     3481 agctatatta ttagttataa cggtagtaaa acgattaaca tgactaatga agaagtagaa
     3541 gtaagtaaat cgattggtaa gcaagatttc gatgaaattg tagattattg tcgagataga
     3601 ggctttttcg ttcttacata tcatgatggt caaattattt acgacagcga acatgagtat
     3661 atgaatattg aagcagaatt aacaggttta ccgatgaaac gtgttgatga tatcaaagcg
     3721 tatattcaag gcgatgtacc caaggtcatg ggtgtagatt atgtagcgaa tattacagaa
     3781 gctagaattg atttgaatgg tgtgttcaat gataatgtag atgctacgac aagtaagcca
     3841 ttcttcttag aatttatggc caaagacgtt tcaaaaggta atgcaattaa agcgttatgt
     3901 cacaaattgg gatattcggt ggatcaagtc attgcttttg gtgatagtat gaatgataaa
     3961 tcaatgtttg aagtcgcagg tctagctatt gctatgggga atgcatcaga tgaacttaag
     4021 caatatgcaa atgaagttac gttggatcat aatgaaaatg gtattccaca tgcgctcaaa
     4081 aaattgttat aaattttaaa ataagcctta acacatgata tttgaataag atatcttgtg
     4141 gttaaggctt tttatttttg tgaaaatgac ttcagttata ctatggagga tttgaaatac
     4201 atattttaga ttagtaatga tatcaaacga atagagtaaa tgtatatttt ttgaataaat
     4261 caagtattaa ctagtcacgg aaggtagata aataaatttg atcattctga ataataacga
     4321 atgcattaaa aaatgcagtg acaatctaac attaattgtt aaaatcactg caagcatctt
     4381 gattttattt agctgtttgt gtccagtcat gcttacctct gaaacgtttt acatgagcat
     4441 atatttggtc agcagtataa tctaaatttg tataaactgt aatactgaac gctgattggt
     4501 tttcagcaaa tgattcttcg gaaaataatt cctttaagtg cataaataaa tcattcattt
     4561 gtttatggtt taaaccggga tattccctta aagttctctt taataattgt ccgttctttt
     4621 cttctaatgc ttgaactaca ataacaaatc tttcatcttg ctttagtaca tcatcaaata
     4681 aatttgtttg tccaatcatt gaatttcacc cctgaaaagt tattgttatc tatattatac
     4741 acgatatgca aaatatctaa aataaagaat ggtaacatgg taataataaa ata
//
LOCUS       DAKTIP010000184         4774 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAKTIP010000184 DAKTIP010000000
VERSION     DAKTIP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.187"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            301..456
                     /locus_tag="O0305_002326"
     CDS             301..456
                     /locus_tag="O0305_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275441.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            464..985
                     /locus_tag="O0305_002327"
                     /pseudo
     CDS             464..985
                     /locus_tag="O0305_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1124..1654
                     /locus_tag="O0305_002328"
     CDS             1124..1654
                     /locus_tag="O0305_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0275442.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1917..4718
                     /gene="clfA"
                     /locus_tag="O0305_002329"
     CDS             1917..4718
                     /gene="clfA"
                     /locus_tag="O0305_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCY0275443.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNTTTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSD
                     NDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSE
                     SDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLL
                     ASIGSLLLFRRKKENKDKK"
BASE COUNT         1744 a          733 c          922 g         1375 t
ORIGIN      
        1 tgtattgttg caataaatta atgaaactat aattactaat attatattac ttttattgat
       61 agaaatatat tactttttta aaaaaacttg taatatatcg aaagatttaa atgtaaaatt
      121 ttgatttgtt aagaaattac gtttgtaaaa ataaaaaaat caacttattt gtatgagata
      181 aatatgtatt gaagaagacg tgttattaat ttggaaaata ctggtcaaag atgggagctc
      241 ttaaaagcgt tattgtattt tttagtcaat acaaatagat tgccgtaata ataatcgtac
      301 ttgatggtta aaaaattact taaggctata aagcaaaact ttttatatga gcagtcgaat
      361 ataacgttta aaatgattgt ttttggatat aaacgattaa gtaaaatgct ttttcagttt
      421 gaaattaatc atataaattt cttatgggag ggttgatatc ttaatgatta acattatttc
      481 agctatagga tctattggaa catttattat ggctttattt tattttgtat cagtttcagt
      541 tcaactttat caaatgaaaa ttagctttct gccagcttta ggttttaacc aaattttatt
      601 agaaagggag gaggatcaac ttaatataat gaattcggca acagaagagc atcatcataa
      661 agattatatt aaactatata atttaggtgg cggtgctgct aaaaaaattg caatagaggt
      721 tttattgggg aaggataaag tcattcagaa aaaatacgtg catattttac ctagtaaaga
      781 agggtacatg ttaccaatta ataaaaatgt gtacgaagaa ttagaaagaa cgattgagaa
      841 caatggtcat gaagctgatt tgaatgtacg tatgacttat tatcataatg taagtcgcaa
      901 acaataggaa gttatattaa aaggtcaaat cgaccgtttt aatacttata ataataaaga
      961 aatttatgat ttgcagttta tctaaaaatt gatttaagag ggtagttgtt tattgcgaaa
     1021 aatatcattc aattttaatg aaataatggc gtcattacta taaaatatta ctttatgttg
     1081 taatgcattt ttctataaga tagaactaaa aggaggggca aagatgcaaa ttagacaaat
     1141 acatcaacat gactttgctc aagtggacca gttaattaga acggcatttg aaaatagtga
     1201 acatggttat ggtaatgaat cagagctagt agaccaaatt cgtctaagtg atacgtatga
     1261 caatacctta gaattagtag ctgttcttca aaatgaagtt gtagggcacg gtttactaag
     1321 tgaagtttat cttgataacg aggcacaacg ggaaattgga ttagtgttag cacctgtatc
     1381 tgttgatatt catcatcaaa ataaaggtat tgggaagcga ttgattcaag cattagaacg
     1441 agaagcaata ttaaaaggat ataattttat cagtgtatta ggatggccga cgtattatgc
     1501 caatctagga tatcaacgcg caagtatgta cgacatttat ccaccatatg atggtatacc
     1561 agacgaagcg tttttaatta aagaattaaa agtgaacagt ttagcgggaa aaacaggtac
     1621 cataaattac acatctgctt ttgaaaaaat atgatttcaa gctaggatta cattaggtag
     1681 agttcatatt aataataaaa aatgtttgca atcaaatcgt acgttgtcgt ttgtaattct
     1741 taaaatagca ataaataaaa tgtttgttag taaagtatta ttgtggataa taaaatatcg
     1801 atacaaatta attgctataa tgcaatttta gtgtataatt ccattaacag agattaaata
     1861 tatctttaaa gggtatatag ttaatataaa atgacttttt aaaaagaggg aataaaatga
     1921 atatgaagaa aaaagaaaaa cacgcaattc ggaaaaaatc gattggcgtg gcttcagtgc
     1981 ttgtaggtac gttaatcggt tttggactac tcagcagtaa agaagcagat gcaagtgaaa
     2041 atagtgttac gcaatctgat agcgcaagta acgaaagcaa aagtaatgat tcaagtagcg
     2101 ttagtgctgc acctaaaaca gacgacacaa acgtgagtga tactaaaaca tcgtcaaaca
     2161 ctaataatgg cgaaacgagt gtggcgcaaa atccagcaca acaggaaacg acacaatcat
     2221 catcaacaaa tacaactacg gaagaaacgc cggtaactgg tgaagctact actacgacaa
     2281 cgaatcaagc taatacaccg gcaacaactc aatcaagcaa tacaaatgcg gaggaattag
     2341 tgaatcaaac aagtaatgaa acgacttcta atgatactaa tacagtatca tctgtaaatt
     2401 cacctcaaaa ttctacaaat gcggaaaatg tttcaacaac gcaagatact tcaactgaag
     2461 caacaccttc aaacaatgaa tcagctccac agagtacaga tgcaagtaat aaagatgtag
     2521 ttaatcaagc ggttaataca agtgcgccta gaatgagagc atttagttta gcggcagtag
     2581 ctgcagatgc accggcagct ggcacagata ttacgaatca gttgacgaat gtgacagttg
     2641 gtattgactc tggtacgact gtgtatccgc accaagcagg ttatgtcaaa ctgaattatg
     2701 gtttttcagt gcctaattct gctgttaaag gtgacacatt caaaataact gtacctaaag
     2761 aattaaactt aaatggtgta acttcaactg ctaaagtgcc accaattatg gctggagatc
     2821 aagtattggc aaatggtgta atcgatagtg atggtaatgt tatttataca tttacagact
     2881 atgtaaatac taaagatgat gtaaaagcaa ctttgaccat gcccgcttat attgaccctg
     2941 aaaatgttaa aaagacaggt aatgtgacat tggctactgg cataggtagt acaacagcaa
     3001 acaaaacagt attagtagat tatgaaaaat atggtaagtt ttataactta tctattaaag
     3061 gtacaattga ccaaatcgat aaaacaaata atacgtatcg tcagacaatt tatgtcaatc
     3121 caagtggaga taacgttatt gcgccggttt taacaggtaa tttaaaacca aatacggata
     3181 gtaatgcatt aatagatcag caaaatacaa gtattaaagt atataaagta gataatgcag
     3241 ctgatttatc tgaaagttac tttgtgaatc cagaaaactt tgaggatgtc actaatagtg
     3301 tgaatattac attcccaaat ccaaatcaat ataaagtaga gtttaatacg cctgatgatc
     3361 aaattacaac accgtatata gtagttgtta atggtcatat tgatccgaat agcaaaggtg
     3421 atttagcttt acgttcaact ttatatgggt ataactcgaa tataatttgg cgctctatgt
     3481 catgggacaa cgaagtagca tttaataacg gatcaggttc tggtgacggt atcgataaac
     3541 cagttgttcc tgaacaacct gatgagcctg gtgaaattga accaattcca gaggattcag
     3601 attctgaccc aggttcagat tctggcagcg attctaattc agatagcggt tcagattcgg
     3661 gtagtgattc tacatcagat agtggttcag attcagcgag tgattcagat tcagcaagtg
     3721 attcagactc agcgagtgat tcagattcag caagcgattc cgactcagcg agcgattccg
     3781 actcagacaa tgactcggat tcagatagcg attctgactc agacagtgac tcagattccg
     3841 acagtgactc agattcagat agcgattctg actcagacag tgactcagat tcagatagcg
     3901 attcagattc agatagcgat tcagattccg acagtgattc cgactcagac agcgattctg
     3961 actccgacag tgattccgac tcagacagcg attcagattc cgacagtgat tccgactcag
     4021 atagcgattc cgactcagat agcgactcag attcagacag cgattcagat tcagacagcg
     4081 attcagattc agatagcgat tcagattccg acagtgactc agattccgac agtgactcgg
     4141 attcagatag cgattcagat tccgacagtg actcagattc cgacagtgac tcagactcag
     4201 acagtgattc ggattcagcg agtgattcgg attcagatag tgattccgac tccgacagtg
     4261 actcggattc agatagcgac tcagactcgg atagcgactc ggattcagat agcgattcgg
     4321 actcagatag cgattcagaa tcagacagcg attcagaatc agacagcgat tcagattcag
     4381 acagcgactc agacagtgac tcagattcag atagtgactc ggattcagcg agtgattcag
     4441 actcaggtag tgactccgat tcatcaagtg attccgactc agaaagtgat tcaaatagcg
     4501 attccgagtc aggttctaac aataatgtag ttccgcctaa ttcacctaaa aatggtacta
     4561 atgcttctaa taaaaatgag gctaaagata gtaaagaacc attaccagat acaggttctg
     4621 aagatgaagc aaatacgtca ctaatttggg gattattagc atcaataggt tcattactac
     4681 ttttcagaag aaaaaaagaa aataaagata agaaataagt aataatgata ttaaattaat
     4741 catatgattc atgaagaagc caccttaaaa ggtg
//
LOCUS       DAKTIP010000185         4683 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAKTIP010000185 DAKTIP010000000
VERSION     DAKTIP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.188"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(90..998)
                     /locus_tag="O0305_002330"
                     /pseudo
     CDS             complement(90..998)
                     /locus_tag="O0305_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            complement(1223..1435)
                     /locus_tag="O0305_002331"
                     /pseudo
     CDS             complement(1223..1435)
                     /locus_tag="O0305_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
     gene            complement(1510..3045)
                     /locus_tag="O0305_002332"
     CDS             complement(1510..3045)
                     /locus_tag="O0305_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCY0275444.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            complement(3096..4640)
                     /locus_tag="O0305_002333"
     CDS             complement(3096..4640)
                     /locus_tag="O0305_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0275445.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVKYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASSLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVTFDIPQNVSVNEPFEMTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNVTIKVNAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
BASE COUNT         1471 a          914 c          661 g         1637 t
ORIGIN      
        1 aaacatttca actactttta aaaaccaaca aaaaaatact tattttaagt agatgagcat
       61 aagtgaacat agttctttag ttataataat taattcaacc aaaagtcgat ttgtttttgc
      121 aattggtttt catttcctct taaagatatt ttcattaaat ctgtcaaatc aatagacgct
      181 atatttttca acttatctct atatttattt ttagtacgtc tttctaaatt tccccattcc
      241 tcttcttcgt gagttaataa atgaagcatt gctcgttctt gtatattttc aatcattttt
      301 aaattcggtt ttaaaatatg caaatcatca aaacaatctt tccaacaatc aaccatatct
      361 cgttttaatt caatttccac acgccataga aatgttgaat caatttcaac atctgcatta
      421 tctttacgtt cttgttttta ttataaatcc gaataaacct atcactatta cgcacaccaa
      481 aatattttgt ttctggtttt acattacgtc cataaaatat agttttcttt accgacttat
      541 ctgacaatgc ataatagtca tttaaatcaa attcaaaatc aaaagccaaa tctaatctcg
      601 taaaactaac atcgtccaaa taactgatga tattttgttt taaccaaagc acttcatcat
      661 gcgaaagctt attaggatta aattcaacgc gcatactacg tctattccaa agagttgctt
      721 ttattttgtc atattcaata taaacttttt ctttaagagc tttagcttta aagtttgttt
      781 gtaaaatatc ccaaagccga atttcaggat tagtactcat aaaatgtgaa agtctctctg
      841 cgttagacat gctaagattc ccaacaatcg ttatagcgtc aaaagacaat tttggaatag
      901 ctagtgacat cctatgtcga tttaaccggc tattaccgga tattagagta tccagtttta
      961 caaatggatg aaacgaaatt caaaacacta aaaaatatgt tccactaaca gcaaaaaaat
     1021 accattatgt tcctactaaa aaactaaaaa tactggagaa caaatgtcag gatataactt
     1081 aggatactat gtaataaaaa tttacaataa aaaaacagga aaacaaattt caagtaaaag
     1141 aatacccata caaagaggat aaaataaaaa acctcgaact gaaatgatga tcttttcagc
     1201 tcgaggttta aatattggtg ccttatttat atagattcgt tatattatat tctctatttt
     1261 cattaacata atccttaaag agttttaaat taatacctgc tagatgattc aaaaatgttt
     1321 catcaacttt taaataattc aataattttt gtggtgtcag taaatttcta tcaaaataca
     1381 actttaataa actattcatt ttgacaggac gtgacatttc aatcacgtcg tctaaagata
     1441 atactttctc gctttaaaca aaaacaaaaa cttacccgat taaaatcaag taagttttac
     1501 atatatccgt cacttttcaa ctttaaaatc aacaacttgc gggatgcgca tcaaattatt
     1561 atttgataaa aaatcaataa gtaattgtgc gccttcaact tgaatatctt ttacaactgg
     1621 cgcgtcgata tacatatcat actgaccacc gcctactgca cgataattat ttacacaaat
     1681 tgtatatgtc tgctttaaat caactgcgtg accttgaatc atcatattgc tcacacgttg
     1741 tccctttggt cttccaacat gaatggtata acttacgcca ccatatatat cataattaaa
     1801 gtgttgtggt ttgggttcaa ggaagtctgc gctcacacta acttcatcat ttttcacgtc
     1861 aaaatattct gctgatcgtt caatggcttc tttaagtttg gcaccactta cagctaaaac
     1921 tttaaatgta tttggaaatg ggtaattgtt aataacatct cgcatcgtca cgacttgctt
     1981 gaaaccacta gcagaatcaa acaaagctgt acaggcaaca tctgcgtcac ttttttctaa
     2041 taaagcgtaa ttcataaaat ttgtaaaagg atgcggtgcc acacgtgcct caaatgcatg
     2101 attaatcgtc atatcatatg gcaatgtagt aatttcgtaa tctaaccagt cctctaactg
     2161 ctttcgtaaa tgttggtcat cttcatcaat agtaaatgtg gaatcatcta taacaggaag
     2221 taattcacat gattcaacgg atagattttc atattcatca gtactcaaga ctactctgcc
     2281 tacagttgta cctctcgtac caggttgaat cacagccgtt tgcttaaacc tttcagcaat
     2341 ttgtcgatgt tggtgacccg taataaagat atctatatct ttagaaaacg cttctaacat
     2401 ggcatatcct tcattttcac ccgttaatac ttcggtcggc gtaccacttt ctaaatcctt
     2461 ttcaaatcca ccatggtaac aaaccacaat gatatctgca tgtcgcttca tttcaggtaa
     2521 gtattgttga agtatttcaa aagcactatg aaacgtaagt gactgaatat gctctggttg
     2581 ttcccaatgg ggaataaatt gtgtcgttaa acctatcaca ccaacagttt gatctccaac
     2641 ctgaaaatac ttcacaccgt tatcagtcaa tgtactatca ttttcatata tattagcgca
     2701 caaaactgga taattgagtc tgcgtaaagt gtcttttaag tatggtaatc cataattaaa
     2761 ttcatgatta ccaagcgtac caaagtcgaa tgccattcga ttataaaaat caactaaagg
     2821 ctggctactg ccgctatgcg cgattaagta attacaaaat ggtgaccctt gcaaaaaatc
     2881 accattatct attttaaaac tttggtcata ctgccttctg tcttgttcta taacatgatt
     2941 cgctagtaac aatcccatag gttgatattg atttctactc gtaaaatctg ttgggaaaat
     3001 ataaccatgt acgtcactca cgacataaaa tgctatgttt gacatcctca ctcactcctt
     3061 caatcacaaa catctttctt atttctatta tatatttatt tgaagtctgt tgtaatcaag
     3121 gttttgtcac cgagttttaa acgaatcttt gaaccttcca tactttcaag tactttagca
     3181 ttgaccttaa ttgtgacatt tccgttttca tctgctttaa ctgttggcaa agtactgtaa
     3241 cctggtgggt tataatcgtt atctttactt gaaaattgtc cgatttgacg tccgccttct
     3301 ttgtaaatac caactctaag attttcaagt gtttgatttg cttcaaatcc ttttaaatgt
     3361 attgtcattt caaatggctc atttacagaa acattttgcg ggatatcaaa tgttactttt
     3421 tcgttctgat ttggtggtgt atgatcatct ggtgtgtttg gctgaggatc tgcgcctttt
     3481 tcgctgccat aactacctgc tttaaatgtt gttggatcat accatttata accactcggc
     3541 ggttgtgacc atggctcttt ttcaggctca gttgaacgct ctggtcgttc aaaatcaagc
     3601 aacttagtct ttgtatctaa tgttaggcta ctcgccttaa gtgatttccc atcattatct
     3661 ttagacatcc aagccgttat attatttaat agcttaccgt tgtcttgttc tttaaaacca
     3721 tcatatgttt tcttcttttc tccattatct tctcttacat atttgggcga actatcttcc
     3781 acaagtgatg aatcaccgat aaatgctgct ttaccttttc caactttaga aattgctaca
     3841 taggggcctt ctgctttacc gcccccatta taaatacctt gatctacagc atgtgaccat
     3901 ttacttttcg ctggcaattg ttctggtgta tacacaatac cttttgcttt ctctggatta
     3961 gtaattgcta atgtcgatcc ggcatgcata gagacagatt tcacaccttc agtaataccg
     4021 aaactttctt ttgaagaaac aatattgctc gtatttaaat cacctagtgc attatatcga
     4081 aaacgtacgc caaagtttgt agataaccaa tctgaacttt tcacaccttg cattgcagta
     4141 gaactttttt cttctgcatt catacctttc gacatatctt catatgctcc acgtcgataa
     4201 ccattcattg cctccgatga atcaatacga tttaaatttc ggtcagcatt gtaatgatct
     4261 gaaataaaga caacattgcc accttgtttc acatatttaa caattgctgc ctgttctgat
     4321 tctttgaaag gaatgttagc ctcaggaatt acaaatattt tggaactttt caaacttgct
     4381 tctgttatgt tcgaatgacc atcaatagct ttaacgtcat aaccttgttt ttgtattgaa
     4441 tccgcataat ctgaaaatgc accatcacta acccaatctg cagcaccagc tgtttgacca
     4501 tgagaacgat cgaataatac cgttcgctgt tgctttgtag gttgcgattc atgcgttata
     4561 gctaaagatt gcggtaaagc acttaatgat accgttgcaa caattgcaga gacagttaat
     4621 gacttatata tttttttcat tttgtgaggc tccttttaaa ataaatttgt tcttgaatta
     4681 tag
//
LOCUS       DAKTIP010000186         4595 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAKTIP010000186 DAKTIP010000000
VERSION     DAKTIP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.189"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(408..506)
                     /locus_tag="O0305_002334"
     CDS             complement(408..506)
                     /locus_tag="O0305_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275446.1"
                     /translation="MNWIIELDLWLWCVGAINLINAVNDYANEILL"
     gene            complement(711..1307)
                     /gene="recR"
                     /locus_tag="O0305_002335"
     CDS             complement(711..1307)
                     /gene="recR"
                     /locus_tag="O0305_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCY0275447.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            complement(1314..1631)
                     /locus_tag="O0305_002336"
     CDS             complement(1314..1631)
                     /locus_tag="O0305_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCY0275448.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O0305_002337"
     CDS             complement(1721..3418)
                     /gene="dnaX"
                     /locus_tag="O0305_002337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCY0275449.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMKQLEQELKTLKAQ
                     GVSVAPVQKSSKKPARGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            complement(3487..4011)
                     /locus_tag="O0305_002338"
     CDS             complement(3487..4011)
                     /locus_tag="O0305_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0275450.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            complement(4149..4250)
                     /locus_tag="O0305_002339"
     CDS             complement(4149..4250)
                     /locus_tag="O0305_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275451.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            complement(4319..4588)
                     /gene="ffs"
                     /locus_tag="O0305_002340"
     ncRNA           complement(4319..4588)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0305_002340"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         1409 a          995 c          615 g         1576 t
ORIGIN      
        1 aacatttaag tcaataactt tttaaaatta attttcgtta gtaattcaag ttatttctta
       61 agttgagttg ttttgtttgc ctcaacaaat aaatactata caggctttca aactgcttaa
      121 caacagtaaa aattacacct ttgtcatctg tactgaacgc ccttatccta aaaatcgaac
      181 ttaaaaaatc cccacttata aatagtacgt tttttagaac aagaaacaga tttttctcta
      241 accatatcaa tcaaaaaaaa cgcaaaacag aacgttattt ttttaccaga aattaaaaat
      301 aaatttacaa gttttaaata atctctcaaa acaccgctat acaggctgat ttaataagct
      361 ttagaccaaa gaagtacaac ttactaagcc agcatctatc atttcaatta caaaagaatt
      421 tcatttgcat agtcattaac cgcatttatc aaattaatag caccaacaca ccataaccat
      481 agatctaatt cgataatcca attcacatca ttactattag aagtaacaaa tcacaaccaa
      541 cggcttgctt cctaaagatt acccatccct cagtaaaact aggtaaacca taccaccgcc
      601 acaccgcaaa taaagtagta catcacacca caccataaaa aaagccacaa tgattacact
      661 gtgacttttc ttactatagt attaaaatca aaaatgttta atagaagaca ttacatttct
      721 gttctacctg cgattgcttt agataatgtt acttcgtcag catactctaa atcgccacct
      781 accgataacc cttgtgctaa tctcgtcact ttgataccta taggcttaac taatctagaa
      841 atatacatgg ctgtagattc cccctctaag ttcgggttca tagctaagat taattcgcta
      901 acttcatcgt ttttcaagcg ttcaatcaat gaaggaatat taatatcttc tggtccaatg
      961 ccatccatag gcgaaataga cccatgtaaa acgtgatata aacctttgta ttctctcatt
     1021 ttttccatag ctatgacatc tttgtcatct tccacaacac aaataactga acgatctctt
     1081 tgcttatctt cacaaatata acatggatca ttttcagtaa tgtgaccaca tacgctacaa
     1141 tatgttaatt ctctcttaac atctactaat gctttggcaa actgaacaac atcgtcttct
     1201 ttcatatcta aggtatgaaa agccagacgt tgggctgtct ttggaccaat gcctggcaat
     1261 ttcataaagc tatcaataag ttttgatata ggttctggat aatgcatcta tgatcacatt
     1321 ccagggatgt ttaagccttg agtatgttta cctaaacgtt cttgagtaag ctcatcagct
     1381 ttattcatcg cttcattagt agctgctaac actaagtctt gtagcatttc aatatcgtct
     1441 gggtctacag cttcttcttt gatttcaacg tcgacaactt ctttatgacc agttacagta
     1501 actgcaacca tgccaccgcc agctgttcct acaatacgct cttctttaag tttttcttgt
     1561 tcttgagcca ttttcttttg cattttttgc atttgtttca tcatttgttg catgtttccg
     1621 ccaccgcgca tattccattt cctccttgaa aatcaataaa tatttatcaa taaaatgatg
     1681 tttctttcat tatacatacg attatatcgc ttgtcatgta tcactcttca tctatcacat
     1741 gtacagtttc ttcaccgaaa agatcttttg ctttttgagc aatatctgtt tgttgtgctt
     1801 gttgctttgg catatcatcg ccttcgtttt tacgattttg taaatattcc gttcgaactc
     1861 tttgccattg atctgatggt acaccaacaa ctttaacgtt tttattaacg atattacata
     1921 caacactttc tatactacta cgtttctcgt cgtctttatt gacgatttca caatggatct
     1981 cttcctcaaa tttcacaagt acgtgatctt cacttgccgc cacaggttcc gaattttgca
     2041 ataaactaac gagtgatttt ttatcattat ttttggcatg atcaatcact tcttgccaat
     2101 gatctttcaa caatttgata tctgccttat tcgctttatc tagcactttt gcaatttgtt
     2161 gcattgaaaa tgcattttta gatttttgta tgcctctcgc aggctttttc gaagattttt
     2221 gaacaggagc gacactcact ccttgtgctt ttagtgtttt tagttcttgc tctaactgtt
     2281 tcatacgttg caacaataca tctgtgtttg gcgatgaagc aatttgtgct ggttcagcta
     2341 cattcgcaat cacttgtggt tgacccttaa tctgctcagc taattttact aacaacactt
     2401 caaaatgaac gttttgattc acactaaaac gaatcgacac taatgtatca ttaataagat
     2461 caatcatttg atataacata tctaattcta agttcatcag tgctcgatac tcagtatctt
     2521 tctcagatgt tttattcata atcgtatctc tgacaaaata aatcatatca tttattaggc
     2581 gattcacttc tttaccttct gttataaact gatggtattt tttaaaagat gcttgtacgt
     2641 caccttgtac aatatcatca aacaagtgat ccaacgcttc atcatgtacg ctacctgtga
     2701 cattcaacgc atcttgcaat gttaacgtac catcaccaaa tgcaatagcc tgatccataa
     2761 tacttaatgc atcacgcata cccccttcag acgctttagc gataaatgcc aaggcttcat
     2821 cttcacattc aatttgttgt gcatctgcta caaattttaa acgttcaaca atttgatcta
     2881 ggctaattgc tttaaaatca aaacgttgtg ccctagaaat gattgttgga gggattttat
     2941 gtggttctgt cgttgccaat ataaaaatag cgtgtgctgg aggttcttct aacgtcttta
     3001 aaagggcatt aaaagcacct gttgttagca tgtgcacctc atctataata taaactttat
     3061 atttcgattc acttggtgca tatttaactt tgtctctaat atttcttatt tcatcaaccc
     3121 cattattact agcagcatca atttctatca catctgaatt agtcccctgc gtaatgcctt
     3181 tacaaatatg acattcatta caaggttctc catcagtgct atttagacag ttgattgctt
     3241 tagcaaacac tttggcaata ctcgttttcc ccgtacctct cggaccacta aaaatataag
     3301 catgcgactg tttttctttc gaaatcgcat tgcgcaatgt cttcgtgaca tgttcttgtc
     3361 cgacgacatc ctcgaaactt tggggtctgt acatacgata taaggcttga taattcaagt
     3421 tagcacctcc ataaacaatt acctctcatt atagcatgat aataccttta cttcttaatt
     3481 gaacaattaa taaaaatgtt ctggaaattt tacgattccg tgtggtgcat ccgtcaaatt
     3541 atcccataaa tattttacaa gtaacggtgc atcaccagat tctaatttaa tgtcatgctc
     3601 agcagcattt tgataaccca acttttcaaa gtagtcaaaa caatggtcta caacaaccgt
     3661 actatactct tgtgctttgg cacgctcttc tactgcttga accaagccac gacctaattt
     3721 ttgtccacgt aattcaggat gaactgataa agaggcaatc gccaaaccat aatacgtctt
     3781 atcatcacta ttaatttcta cttcaattaa taaaacgtgt ccaacgacat cgttattttc
     3841 atttttcgct attacttcta attcaaaatt atagcaagga gattttctta aatgttttac
     3901 tttcgcacgt gcttgccaac tcgtttcagg attatcatca aaactttctt caatactatt
     3961 taaagattta tcataatcta actctgttaa agtacttaaa tatatttgca tatgtcctcc
     4021 gtaggcattt gattgtcaat aatcataacg tatctcatta ataattctat tgtaagatac
     4081 tccccctcat ttcaccatca tttctttatc atcaaattat aacttcttac ttttcattga
     4141 cacaaaaatc attcaaactg cacatcgagt tcacttgaat caaacttcac atataaaaaa
     4201 gctacttccc acaaacatgt tccacgtata atacgctgaa ttgtcttcaa gaaagtagct
     4261 tctataatta tatattttca actcttaata atcggtttaa tatttaaaaa ataaaaaacc
     4321 gtgcacctaa gcatcgaacg taattcacga acgtaaccaa agtcgttagc taaatctcgg
     4381 ctaccctacg gcacatatga tgatccactt aatgctgctt ccgtcaggac ctgacatggt
     4441 tcatgggttc atattgcata ggaccgaaat cttcaaacac tacgtgcttt gggcagactt
     4501 cgcaaaaata cggcctcaac aaaggaatta agcctcgcat aaagcggatt tcgagtacag
     4561 ggaaccgcta cctccccacc tagcacggca agata
//
LOCUS       DAKTIP010000187         4576 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAKTIP010000187 DAKTIP010000000
VERSION     DAKTIP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.190"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(78..269)
                     /locus_tag="O0305_002341"
     CDS             complement(78..269)
                     /locus_tag="O0305_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275452.1"
                     /translation="MPEQLSVRKWRLVRDLKQQEVADILGVNAKTVGHWEKDDTNLSN
                     VTVYALAKLYDIEVDQIKV"
     gene            430..759
                     /locus_tag="O0305_002342"
     CDS             430..759
                     /locus_tag="O0305_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275453.1"
                     /translation="MENNKVRKILSENLQELMNDKNIDQRELAEAIGVSQPTVSNWIQ
                     QTKYPRIKRIQQLADYFNVPKSRITESKKDIHQETIAAHFDKEGLTEEEIEEVNRFIE
                     WVRNRDK"
     gene            772..1233
                     /locus_tag="O0305_002343"
     CDS             772..1233
                     /locus_tag="O0305_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCY0275454.1"
                     /translation="MGLYEELCINNEKIKIEETDQLPNFQPGCYMNGKIYIRRNLSEV
                     RKAEVLYEQLAHHKLTYGNILDQTKWINRKFENYARRHGFTSAVPLHEIVEAHNYGVR
                     NLYELSEYLQLSESYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            1251..1685
                     /locus_tag="O0305_002344"
     CDS             1251..1685
                     /locus_tag="O0305_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275455.1"
                     /translation="MKKVIGLLLVSTLALTACGEKEKPKKEENKKSQTQNHKDSKPKT
                     QQEKMKKVEDKNPPNNSIQNNSNNQNQSQNNQLNNNSDPSNNTPANINENDSQNTNLN
                     DEYVVSPGWTKDEQAKAFEEYKKGKEEEARAGASAVPGANIN"
     gene            1714..2109
                     /locus_tag="O0305_002345"
     CDS             1714..2109
                     /locus_tag="O0305_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275456.1"
                     /translation="METNKTIDLMNYVEFPKRYTEAKGKLVAQPITTINSARRVENED
                     MTVCYILDQDDDVMDFIFDRDIITVYCPENGTATDEYFCEIIFNSDDTFTLKRLSNYV
                     TIKDRSYPMSKINDVNITGKVVRLFRDFK"
     gene            2199..2345
                     /locus_tag="O0305_002346"
     CDS             2199..2345
                     /locus_tag="O0305_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275457.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(2342..2956)
                     /locus_tag="O0305_002347"
     CDS             complement(2342..2956)
                     /locus_tag="O0305_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCY0275458.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            3082..4287
                     /locus_tag="O0305_002348"
     CDS             3082..4287
                     /locus_tag="O0305_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCY0275459.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            complement(4330..>4576)
                     /locus_tag="O0305_002349"
     CDS             complement(4330..>4576)
                     /locus_tag="O0305_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275460.1"
                     /translation="LIPNVTCNYIKTKLEELDNTLGKWEPFLNDLKAVSPILDNHLDD
                     IVKNMKPLIVQMIFEPSHYDDMFISRKALTPVFSSVL"
BASE COUNT         1874 a          689 c          686 g         1327 t
ORIGIN      
        1 ttcctccttt taagttgttt atgtccattt gttacattac ctaaaagtta taaaaagtta
       61 tacgaaggtc aaaaaattta gacctttatc tggtctacct caatatcata taactttgct
      121 aaagcgtaaa ctgtaacatt acttaaatta gtgtcatcct tttcccaatg accaactgtc
      181 tttgcattga cgcccaatat atctgctact tcttgctgtt tcaagtccct tacaagtctc
      241 cattttctta cggataattg ttccggcatg tcgtccacct ccctttcaca cgtataactt
      301 tatattactt tgtattactt gtcaacagat aaagttatat ttcttcataa aaagttataa
      361 aaagtattgt aaagtaatgt cggttatggt aagttaatat tacattaagt aatattaaag
      421 gagacaacta tggagaataa taaagtcaga aaaattttat ctgaaaacct tcaagaactt
      481 atgaatgata aaaatattga tcagagagaa cttgctgaag ctattggagt ttctcaacct
      541 acagtctcca attggattca acaaactaaa tatccacgaa ttaaaagaat tcaacaactt
      601 gcagattact tcaatgtacc gaaatcaaga attactgaat caaaaaaaga tatacatcaa
      661 gaaacaattg ctgctcattt tgataaggag ggattaactg aagaagagat tgaagaagta
      721 aatagattca ttgaatgggt tagaaataga gacaaataaa gggtgtttat attgggatta
      781 tacgaagaac tttgcataaa taacgaaaaa ataaagatag aagaaactga ccagcttcca
      841 aatttccaac ctggatgcta tatgaacgga aaaatttata taaggcgtaa tttatcagaa
      901 gtacgtaaag cagaagtgtt atatgagcaa cttgcccacc acaagttgac gtatggcaac
      961 attttagacc aaacaaaatg gattaataga aaatttgaaa attatgcacg tagacatggt
     1021 tttacttcag ctgtaccgct acatgaaatt gtagaagctc acaactacgg tgttcgtaat
     1081 ttgtacgagt tgtcagagta tctgcagttg agtgaatcat acatactaga agctatagaa
     1141 caatataaaa agatatatgg tattggaact cactatggcg agtattctat tacgtttgag
     1201 ccgttgagag tttttaaata taaggaaata taaacaaagg agagatacat atgaaaaaag
     1261 taatcggact gctactagta agtacattag ctttaacagc ttgtggtgaa aaagaaaaac
     1321 caaaaaaaga agaaaataaa aagtcacaaa cacaaaatca caaagatagc aaaccaaaaa
     1381 cgcaacaaga aaaaatgaaa aaagttgaag ataaaaatcc acctaataat agcatacaaa
     1441 ataattcaaa caatcaaaac caatcacaaa acaatcaact taataataat tcagatccat
     1501 ctaataatac tcctgcaaat ataaatgaaa acgattcaca aaatactaat ttaaatgatg
     1561 agtatgtcgt ttcgcctggc tggactaaag atgaacaggc taaagctttt gaagagtaca
     1621 aaaaaggaaa agaagaggaa gcaagagctg gtgctagcgc agtaccagga gccaatatta
     1681 actaataaaa caatataaga aagaagagct aatatggaaa caaataaaac aatcgattta
     1741 atgaattatg tggaatttcc aaaaagatac acagaggcaa aaggcaaatt agttgcacaa
     1801 ccaataacta ctataaatag cgcaagaaga gttgaaaatg aagatatgac tgtttgctac
     1861 attttagatc aggatgatga tgtaatggac tttatctttg acagagatat aattactgtt
     1921 tactgtcctg aaaacggaac tgcgactgat gaatattttt gtgaaattat atttaactca
     1981 gatgacacat ttaccctaaa gcgattatct aattacgtta ccattaaaga tagaagctac
     2041 ccaatgtcaa aaataaatga cgtaaacatt acgggcaaag tcgtcagatt atttagagat
     2101 tttaaataaa cttggcttta attacgatta aaagtaccta tatagcgtga cgagaaaaag
     2161 gattaaaaaa aatcaaaaaa cgcctactag tgtagacgtt gaatggtggt gagaatttta
     2221 tggtagataa aaacaaaaaa caagaaacta ctcgtagtaa cccattaaac aaaagttttg
     2281 aaaagtcagg cgccagcgaa aaattaaaaa gcactttatc agaaaaagct aagaaaaaag
     2341 attagtattc attcattaaa tataaatcca atttaatttg ttgtttaagg tctacaagcg
     2401 tatgtttaat atacaattca tcgtttgacg gtaaatcaga tactttgaaa tcttgtcgct
     2461 caacttctag taaatcgaaa tcgctaccag ctgaattata ggttttaagt tcaccctctt
     2521 caatgattct gttttcaaag tcttttataa ctataaatac tggtttaccg ttgttattaa
     2581 acaacttgtc tcttttgtct aataagctta tacaatccaa tttcataaac tttctggtta
     2641 tattaattaa ccagataata aatttaacaa ttaaaggatt aaatacaaac actgttaaaa
     2701 caaaaataaa tagaaacaaa atatttgctt ttagacctgt aagcaactga attaaattca
     2761 aattttttaa atcaacatta ttaaaaatta taaaactata aaaccatatc aaacatgttt
     2821 caatagaaaa aatcaataat acaggagtat tgataacctt gtttttttca ctaactaaac
     2881 ctatcattgt tagatattta tatggtatgt aacctaaaac tcctgtaaga agaagcgccc
     2941 ctagaaattg agtcatctta tcacctactt tttattttat tataacacat ttagtaccta
     3001 gtactaaatt acgggtagcc cgactaccct tattattttt taatatttta tagaacatac
     3061 gttcttgcag gaggtataaa catgtggatt gaaaaattta aaaacaaaaa taacgaaact
     3121 aaatacagat actacgagaa gtataaagat ccatacacag ataaatggaa gcgcgtaagt
     3181 gttgtgttga acaagaatac aaaacaatct caaaaagaag caatgtttcg tttagaagaa
     3241 aaaataaaag aaaaactgaa caacaagtcg tcaagcgaat taaaaacttt gacttttcac
     3301 gcgctattag atgaatggct tgaatatcat ataaaaacat caggttcaaa gttgactact
     3361 cttaataata taaaaataag aattagaaac attaaacgat acagctctga gaacttgctt
     3421 ttaaacaaac tagatacaaa atatatgcag atatttatta ataaattatc agatatctat
     3481 tctcaaaatc aagtaacccg tcaactcgga gatatgaaag gagctattaa atatgcagtt
     3541 aaattttaca attatccaaa tgaatatttg ttaactaatg tcaaaattcc taaaagaaga
     3601 aaaacaatag aggatatcga aaaagatgaa tctaaaatgt acaactattt agaaatgaac
     3661 caagtcctac agatacgtga tcatatacta aatgataata agttacacaa gcgaaatcgc
     3721 attttaattg ccagcatctt agaagtacag gctttaactg gtatgcgcat aggagaacta
     3781 caagcactgc aggaaaaaga tatagattta ttaaacaaaa ctatcaatat aacaggtaca
     3841 attcaccgca ttaaatacga ggaaggattc ggatacaaag acactacaaa gactataagt
     3901 tcaaaaagaa gtatcagcat caattctaga accgtagaaa tttttaaaaa gataatactg
     3961 gaaaacaaaa tgttgaaaag atggaattcg agctatgttg acagagggtt catattcaca
     4021 acaaaaaaag ggaatccttt atgtaataat caaatcgccg gtgtgcttaa gaaaactaca
     4081 aaagctttaa atatgaataa gaaagttacc acgcacacat ttagacatac acacataact
     4141 ttattagtag aaatgaatgt ttctttaaaa gcaattatga aaagggtagg acatgtagat
     4201 gaaaaaacaa ccattcgcat atatactcat gtaactgaaa aaatggatag agaactaact
     4261 caaaaactcg aaaacattcc aagttagctt aaatccgccc tttttttgcc cttatatttt
     4321 ttacaagctt tataaaacgc ttgagaacac tggcgttaaa gcttttcttg aaataaacat
     4381 atcatcataa tgtgatggtt caaatatcat ttgtacaatc aaaggcttca tgttcttaac
     4441 aatatcatct aaatggttat ctaaaattgg tgacactgct tttaaatcat taagaaaagg
     4501 ctcccatttg cctaaagtat tatctaattc ttctaattta gttttaatat aattacaagt
     4561 tacattagga atcagg
//
LOCUS       DAKTIP010000188         4475 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAKTIP010000188 DAKTIP010000000
VERSION     DAKTIP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.191"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            162..1337
                     /gene="rpsA"
                     /locus_tag="O0305_002350"
     CDS             162..1337
                     /gene="rpsA"
                     /locus_tag="O0305_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCY0275461.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
     gene            1559..2869
                     /gene="der"
                     /locus_tag="O0305_002351"
     CDS             1559..2869
                     /gene="der"
                     /locus_tag="O0305_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCY0275462.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEAYVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTGAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2886..3884
                     /locus_tag="O0305_002352"
     CDS             2886..3884
                     /locus_tag="O0305_002352"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCY0275463.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4055..4327
                     /locus_tag="O0305_002353"
     CDS             4055..4327
                     /locus_tag="O0305_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCY0275464.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1598 a          599 c          900 g         1378 t
ORIGIN      
        1 tggttgtaat aaatatttat cgatatgacg acttgaatat gataaagtga catatttatg
       61 tatatgacta tttcgcaaaa tgtaatcgag gtagaatttc ttgacaattc tgtcagttta
      121 taagatgtta taaatatgta gtgtataagg aggcaaacaa gatgactgaa gaattcaatg
      181 aatcaatgat taacgatatt aaagaaggtg acaaagtcac tggcgaggta caacaagttg
      241 aagacaagca agttgttgtt catatcaacg gtggtaaatt taatgggatt attcctatta
      301 gtcaattatc tacgcatcat attgatagcc caagtgaagt tgtaaaagag ggcgacgaag
      361 ttgaagcata tgtcactaaa gttgagtttg atgaagaaaa tgaaactgga gcttacatct
      421 tatctagaag acaacttgaa actgagaagt cttatagtta tttacaagaa aaattagata
      481 ataatgaaat catcgaagcg aaagtaacag aagtagttaa aggtggtttg gttgttgatg
      541 taggacaaag aggttttgtt ccggcttcac taatttcaac agacttcatt gaggatttct
      601 ctgtgtttga tggacaaaca attcgtatta aagttgaaga attggatcct gaaaataata
      661 gagtcatttt aagccgtaaa gcagttgaac aagaagaaaa cgatgctaaa aaagatcaat
      721 tattacaatc tttaaatgaa ggcgatgtta ttgatggtaa agtagcgcgt ttaactcaat
      781 ttggtgcatt tatagacatt ggcggtgttg atggtttagt gcatgtatct gaactttctc
      841 acgaacatgt tcaaacacca gaagaagtag tttcaattgg tcaagatgtt aaagttaaaa
      901 ttaaatctat tgatagagat acagaacgta tttcattatc aatcaaagat acgttaccaa
      961 cacctttcga aaatattaaa ggtcaattcc acgaaaatga tgtcattgaa ggtgtcgtag
     1021 taagattggc aaactttggt gcatttgttg aaattgcacc aggtgtacaa ggacttgtac
     1081 atatttctga aattgcacac aaacacattg gtacgccagg tgaagtgtta gaacctggtc
     1141 aacaagtaaa tgttaaaata ttaggtattg atgaagagaa tgaaagagta tcactatcta
     1201 ttaaagcaac attaccaaac gaagatgttg ttgaaagtga tccttctacg actaaggcgt
     1261 acttagaaaa cgaagaagaa gataatccaa caattggcga tatgattggt gataaactta
     1321 aaaatcttaa actataattt aatatttaat agtcaactcc acatgtttat gattgcatgt
     1381 ggagtatttt tatgtaacaa aatatactcg gaatgataac gtggacaaat ttaactaagt
     1441 gtttaaaaag ataaagtttt aagtgctgat ttttatcatt acagtaataa actcattttg
     1501 aatacacagt ctcatgtgat attattaaaa agatataaga aagagaggaa gttagcttat
     1561 gactaaacct atagtagcta ttgtaggtag gcctaatgta ggtaaatcta caatttttaa
     1621 tagaatagtt ggagaacgtg tttcgattgt ggaagacacg ccaggtgtaa cacgagatcg
     1681 tatttattct tcaggtgaat ggttaacaca tgatttcaat attattgata caggtggtat
     1741 tgaaattggt gatgcaccat tccaaacaca aattagagcg caggcagaaa tcgccataga
     1801 tgaagcgtat gttattattt ttatggttaa cgtgcgtgaa ggattgacac aaagcgatga
     1861 aatggtcgct caaattttat acaaatctaa aaaaccggtc gtattagcgg ttaacaaagt
     1921 agataatatg gaaatgcgta cagacgtgta tgatttctat tcattaggat ttggtgaacc
     1981 gtatccgata tcagggtcac atggtttagg tcttggtgac ttgttagatg cagttgtttc
     2041 tcattttggt gaagaggaag aagatcctta tgatgaagat acaattcgac tatccattat
     2101 tggacgacca aacgtaggta aatcaagttt agtaaatgct attttaggtg aagatcgcgt
     2161 tatcgtttct aatgttgcag ggacaacgag agacgctatt gatacagagt atagttatga
     2221 tggacaagat tatgttttaa tcgatactgc tggtatgcgt aaaaaaggaa aagtatatga
     2281 atcaactgag aaatattcag tattaagagc tttaaaagcg attgaacgtt caaatgttgt
     2341 tttagtggtc atagatgcag aacaaggcat cattgaacaa gataaacgtg ttgcaggata
     2401 tgcacatgaa caaggtaaag cagtcgtgat tgtcgtaaat aaatgggata ctgtggaaaa
     2461 agatagtaaa acgatgaaga aatttgaaga tgaagtacgt aaagaattcc aatttttaga
     2521 ttatgcacaa attgcttttg tgtctgctaa agaacgcaca agattacgta cattattccc
     2581 ttacatcaat gaagcaagtg aaaaccataa aaaacgtgtt caaagttcaa ctttaaatga
     2641 agttgttact ggtgcaattt ccatgaaccc tacaccaaca gacaaaggta gacgtttgaa
     2701 tgtcttttat gcaacacaag ttgctataga accaccgaca tttgttgtat ttgttaatga
     2761 tgtagaatta atgcattttt cttataaacg ctatttagag aatcaaatcc gtgccgcttt
     2821 tggttttgaa ggtacaccaa ttcatattat agctcgaaag agaaattaac gattggggga
     2881 taacaatgac taaaattacc gtttttggta tgggaagttt tgggacagcc cttgccaatg
     2941 ttcttgcaga aaatggacat gatgttttga tgtggggtaa aaatcaagat gctgttgatg
     3001 aattaaatac atgtcataca aataaaaagt atttaaaata cgcgaaatta gatgttaaca
     3061 tcatcgctac ttcagatatg accaaagcaa ttcaatttgc agatatttac ttaatggctt
     3121 tacctactaa agcaatgcga gaagttgctt ctcaaattaa tgataagctg acctctaaaa
     3181 agacttttat acatgttgct aaaggtattg aaaatgggac gtttaaacgt gtgtcagaaa
     3241 tgattgaaga ttctatttca cctgaatata atgcaggtat tggcgtgttg tcagggccaa
     3301 gtcatgcgga agaagttgta gtcaagcaac caactacagt tgctgcttca tcaaaagata
     3361 aaagtgtaag taaattaacg caagatttat ttatgaatga ttatttgcgt gtgtacacga
     3421 atgatgactt gattggtgtt gaacttggtg gtgcattgaa aaatatcatc gcagtagcaa
     3481 gtggtatcgt agctggaatt ggctacggtg ataatgcaaa agctgcatta atgactcgtg
     3541 gcttagcgga aattagtaga ttaggtgaaa agttaggtgc cgatcctatg acatttctag
     3601 gtttaggtgg tatcggtgac ttaatcgtta cttgcacatc aacacattct cggaatttca
     3661 cattaggata taaacttgga caaggtgaat caatggatca agcattatct gaaatgaata
     3721 tggttgttga aggtatttat acaactaaat cagtttatca tttagctaaa gaaaaaaatg
     3781 tggatatgcc aattacaaat gcattatata gagtattatt tgaaaatatc tcagtaaaag
     3841 aatgcgtaaa agatttaatg gagcgcgata aaaaatctga ataaaatgtg tattattaca
     3901 catatacggc taataattac gtaaaatgaa tagaaatggc ttaaattcaa cgtttttgcc
     3961 acataaatga ttgcgattta tgtaatgctt gtgttaaagt tttaccataa tgatataaac
     4021 atatcattat caaacctcag gaggtgaatg tctaatgaac aaaacagatt taatcaatgc
     4081 agttgcagag caagctgatt taactaaaaa agaagctggt tcagcagtag atgctgtatt
     4141 cgaatcaatc caaaactcac ttgctaaagg tgaaaaagta caattaattg gtttcggtaa
     4201 ctttgaggta cgtgaacgtg ctgcacgtaa aggtcgtaac cctcaaactg gtaaagaaat
     4261 tgatatccca gcaagtaaag ttccagcatt caaagctggt aaagcattaa aagatgctgt
     4321 aaaataattt tacttaaaaa gccctgaata agggcttttt attttgcttt taatacttac
     4381 aacctgtaca taaattgtaa tgttcttcta agtttttaat ctttggaaca ttcattgttt
     4441 aagcgttcaa aatattacat gacataatag ttttg
//
LOCUS       DAKTIP010000189         4470 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAKTIP010000189 DAKTIP010000000
VERSION     DAKTIP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.192"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            139..1065
                     /locus_tag="O0305_002354"
     CDS             139..1065
                     /locus_tag="O0305_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCY0275465.1"
                     /translation="MKKVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDSTVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     NLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            complement(1128..1562)
                     /locus_tag="O0305_002355"
     CDS             complement(1128..1562)
                     /locus_tag="O0305_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCY0275466.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(1578..2615)
                     /locus_tag="O0305_002356"
     CDS             complement(1578..2615)
                     /locus_tag="O0305_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0275467.1"
                     /translation="MKKLIIRVVGVLFLVGFLIYLFYSPRLKFDVLENPNKGNKVNRS
                     EQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSY
                     YIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELS
                     DEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNK
                     RPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTE
                     DALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVM
                     RDYYSLIFLEK"
     gene            complement(2942..3403)
                     /locus_tag="O0305_002357"
     CDS             complement(2942..3403)
                     /locus_tag="O0305_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCY0275468.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(3428..4339)
                     /locus_tag="O0305_002358"
     CDS             complement(3428..4339)
                     /locus_tag="O0305_002358"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCY0275469.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLFGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
BASE COUNT         1543 a          796 c          636 g         1495 t
ORIGIN      
        1 aaatgtattt agattatatt aaacatcatt atattaattc aattctcaag agcatcacta
       61 tatgattaac cacacatttc gctgtagtca gaactatgaa tttgttaagc tataaaagta
      121 aacgaggagg aattttttat gaaaaaagta aacagaaaca ttttaaatgc ggtactagtc
      181 ggtgctggat ttttaagtag tctattaatg atcaataaaa ataaagtgat tactaaaaag
      241 caaaccatcc ctgctttttt caaaggcaat gcaccatata tatttgcgca tcgcggtggt
      301 atggctttaa gaccagagca aacgcaactt gcatttgact atgctaaaca attaggcgtt
      361 gatggctttg aaacagatgt gagactcaca aaagatcaac agcttatcgt ttttcatgac
      421 tcaactgttg atagaacgac gaacggctct ggaaaagtta gtgcccatac attagcagaa
      481 ttaaagaaat tagacgcagc ttatcacttc aaagatatca atggactaac accatatcgt
      541 ggtcatgcac acacagctat actaaccttc gatgagttgc tgaagcaata tcctgatatg
      601 tatataaacg tagacttaaa agatgctcct gaatcctatg aaggctcaat cgcaccacaa
      661 attatgtttg atacaattgc agaaaatcaa gcatttgacc gcgttcttgt gacaagtttt
      721 tataaagaac aaatcgttcg ttttaataaa attgcacaag gatccgtcgc aattggtgct
      781 agccaacagg aagttacaga agcattttta aaatatcatt tattaggtgg taggtactac
      841 caaccattag cacaaacgtt ccaaatgcct actcatttta aaggcattga tttgacttca
      901 tctcgtttta tcaaatggtt aaatgatatg aatatcattc caggctatta tggcgtaaat
      961 agtataaatc tgatgaatga tttataccaa aaaggtgcac acacgattgt gacagatcga
     1021 ccagatttag cacaacaatt caaacaaaca atcccgaata aataaaaaca tcgagtcaga
     1081 tgctagttag tggtttcaat aaccaccttt ttctgactcg atgtttttta taatgaacaa
     1141 ttacaggatc cgcccgtcgc acaaccacta tgtgatgttt gaaagaatgg attacctgtt
     1201 tctattttga catgttctga aacagcgtac gcaattttcg taacaacttc atctattaaa
     1261 tgttgaagtg ccatttccct agctttgtaa tgcatcacaa cgtcaagcat ctcatatgct
     1321 cttttgcgtt gtcttgtctc taacataact ttcttataat caggatgata tttcccgaat
     1381 cgcattactt catcatattt ctcttttgat tttaaaaatg attgataaag taaatgtgct
     1441 tcgtcattgt tttcgagcgc ctgtttagct tgctcaaatg atgcataaat atctgatgca
     1501 actatcatat cagctagttc atcaatatca tctaatatcg ctagtgaagc ttcattaatc
     1561 ataaataccc ctcattgtta tttttctaag aaaattagtg aataataatc tctcattaca
     1621 tctccaccca tctcatcgta ttttgaattt aatagacgag atcgatgtat atctgaattc
     1681 atccaactat ggattaatgt tggtacatca ttaaacgcat aaccaacatt ttgagcagtt
     1741 gttttataag taacgtgatt tttatctaat tgccctctta atgcgtcctc tgtaaattca
     1801 acactatcag aaccattaga ggtcgcttca tataagttat tagatgcaat atgtgctaaa
     1861 tcgctattga ttttcaatgg ttttaatcct tttaattttc tcatttcatt cgttacttca
     1921 taaagagaaa ttaattgatt tggattttgc tcaactggac gcttattatg ctcttctgac
     1981 gtagaattag aatttaattg ataaggttca atatctgcta acatttcttt tgttaaaaat
     2041 cgtacactta gcaccttttt cgattgttga tcagaataca cttgtgcata tatgtcgcca
     2101 tatttaatca gtgtttgtgt ttttaaatct tcatctgaaa gttcaaattc atatttttta
     2161 ccatcaactt taaaggacgg ttctggatta atacttgtat gattaaaaat ttctgcagaa
     2221 tgttgtccta tttttaacgg actaacattg actttctcac ctgtagcata cactgaaacg
     2281 atttcttcac gtttagttga aacaatgtaa taactgtttt tgtctttaaa cacataattt
     2341 ttgtaaccat ctctaaaagg gtagacacga tctgcttgtc caaatttaga agtaagcact
     2401 ttaatatctt tacctaccca tgtaccaaca ccttctttag gctttggatt ttcagcatgg
     2461 ttatttgatt tattcacctg ttcagatcta tttactttat tacctttatt cgggttctct
     2521 aatacatcaa attttaatct cggtgaataa aaaagatata ttaaaaatcc aactaaaaat
     2581 aatacaccga cgactcttat aattaacttt ttcatcaatc aaccacctaa aaagtattaa
     2641 tactattgta aaaaacaaca cattaattag caaattttca acactgacat aactgtgtcg
     2701 tttcgataaa acaaaatcat cttcaggcat aattttaacg tcataaagat tttccctaca
     2761 ctctatatca taacctatct ttatgttttc aggttgaatt tcattattaa gattaaaata
     2821 tgtataaaaa aatggacagt taaggtatca aatgaatacc atcaactgtc cgactattct
     2881 tcatcaaaaa acctgataaa acaaattgcc ttatcagatt agtatcattt gtataagcat
     2941 attaatgacc aaatgttgct ttaatcagtg atgtcgtttc tccacctgga tataatacat
     3001 ataataataa gtaaactgct acacctgtaa ttgcagtaca gaaccatata actgaagcga
     3061 atggcccgaa tttgcggtgt acattatatt tatctttaaa tgcagtaata atttgaacta
     3121 ggcctagaat accaccaatt gttgctaaat taatgtggaa aaataagaaa atcgtataat
     3181 atttcttaat tgatgctggt ccgccaaatg ctgtattacc gataaaaatc gttctacttg
     3241 catagattaa gaaaaaagtt aaagcaaaaa cagctgcagc taacataaca tttttgtgtt
     3301 tatttatttc ccttttccaa ataagtctcc aaccaatggc aattaaaatt gcactaatga
     3361 caatacatgt cgtactaatc gttggtaaaa ttggaacgcc catatttttc atcctaactt
     3421 aattaatcta gatcaaagta agtaatgaaa caatcacagc taacacgaaa aagatcacta
     3481 aataatttag tgaatatata aacatttgtg ttgcccattt tgtttgatct gaatttttct
     3541 taaatgttgt taaacctaat gcaatccatc ctaaatttaa taatgtagct aacactacga
     3601 atacgacacc taaatttatt agtaataaag gtactggcaa taaaataatc aaccagataa
     3661 acatactgac acgtgtacgt ttaaagccct taactgatgg taacattgga atatttgcaa
     3721 gtgcatattc atctttacgt ttaatagcta aggcataaaa atgaattggt tgccaacaaa
     3781 atacaactaa aaacagcgca atcgctgtta aactaatttg tccttcaatt gcaacccatc
     3841 caattagtgg tggtactgct ccaggaaaac tcccaatcac tgtgttccat gttgtatgtc
     3901 ttttagacca tattgagtaa taagacacat aacctacaat ccccataaga ccaagtacgc
     3961 ctgatggtat attcaataaa aacaaacaaa tttctccaac taacatcata ccaaaactta
     4021 atagtaataa attttgatct gtaattctat tatttacagt tggtctattt tgtttactag
     4081 gcataatacg atcaatatct tggtcgtaat aattatttaa cgcacatgcg ccacccataa
     4141 ttaaagtaga tccaaatagc attaataaaa tttgaggtat tgatgataag aaggaatgat
     4201 ttgtcattac aactgctagc catgcgcccg caaaagccgg aattaagtta ccttgaacaa
     4261 gtcccatttt aattatctgt tgcaattctt tgaagttaac tctgctaata ttttgtgaca
     4321 aagtatgctc tttgctcata atccccctcc ttaaatttgt tcatataaga ttatgatatc
     4381 ttagattgca taaaaagact aggtttaata aaattaaatt gtgacaaatt aacgacaaga
     4441 gaaaatgtca attttgtgac acaaataaca
//
LOCUS       DAKTIP010000190         4467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAKTIP010000190 DAKTIP010000000
VERSION     DAKTIP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.193"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(44..727)
                     /locus_tag="O0305_002359"
     CDS             complement(44..727)
                     /locus_tag="O0305_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCY0275470.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            complement(746..1933)
                     /locus_tag="O0305_002360"
     CDS             complement(746..1933)
                     /locus_tag="O0305_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCY0275471.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            complement(1977..2492)
                     /locus_tag="O0305_002361"
     CDS             complement(1977..2492)
                     /locus_tag="O0305_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCY0275472.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            2669..2929
                     /locus_tag="O0305_002362"
     CDS             2669..2929
                     /locus_tag="O0305_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275473.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            complement(3061..4431)
                     /locus_tag="O0305_002363"
     CDS             complement(3061..4431)
                     /locus_tag="O0305_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCY0275474.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
BASE COUNT         1588 a          842 c          623 g         1414 t
ORIGIN      
        1 atgtaataaa aaaagcggag tttcccccag cttttctaaa cgactacata aaatataaga
       61 ttgcaattaa atgcaatagt gatgctatta caataaaaat atgccaaatc atatgaaaat
      121 atggtctatt cttttgtgca taaaaccatg caccaattgt ataagacaca ccacctaaga
      181 aaatgaataa tatgaatatc catgatgtgc gaataaaaat aattggtaac aagataatac
      241 ctacccagcc catcactaaa tagacaatta agcttaactt ttggttaact ttgacagcta
      301 tggatttata taaaatcccc caaagtgtcg ttccccataa taaaataaat actgtccaac
      361 ctatccaacc acctacaact gttaacaaaa taggcgtata tgtaccagaa attgctacgt
      421 aaatcatact gtgatcgata atccttaaaa tatatttatg aggtgtttca ttttgcatag
      481 aatgataaat agtagatgaa ataaacatca taaagataga aataacataa atagacatac
      541 tcacagacgc aagcgtacca tgtgcaatat aactgtgcac agccgcatag ggtaacacta
      601 ataatgttaa caaagctgca acaccatgag atgccgcatt accaatttct tctccaaatg
      661 ataaagggat gatatcctta aatgtttcta cgattgtgtc attacttttt ttagttgatt
      721 tggacatcat agtcaccgcc tatcattaaa ttaaacccat tctacgtaaa tcttctgttg
      781 cagttgcaac actatcttta ccttctttat ttttaagagg tgaaaattct tcttctctag
      841 gaacttgtaa cgtgacaaaa ctagttccat aagtaaatat gtcaaaatag aacaattcga
      901 attttaaagt tggttgttca ataacaccaa agttctcatc taattgcttc aaattcttaa
      961 tagctaaatg ataaggtaac tcacgattta cagcgtttaa aataaaacca agtttaaatg
     1021 catgaatacc gatattagca ttgttaaatt gatttgcaac ttctggatct aattctgaat
     1081 actccagtac tgtatcttta caatcgacat taactaaacg tccaacactt tctcctggtt
     1141 ttggttgaat cgttttagaa gtaatatcgt aatcatgttc taccgtaaat cctgcaaata
     1201 gtggatccaa cactcttact aaaacattgt cgatattatt caagaaaata tacttaacgc
     1261 cattattaga catttcttct aagtatcctg ccttgtctaa agatttaaac acgccaccat
     1321 taccattcgg cgtttccata atacgaccct gttgatttaa aattaacttc ccttcttcac
     1381 taagcgccac aatattatct tgtttaaaga aatggattgc ttcttgatca tatccaaaat
     1441 aactatgtgc ttcgaaataa gctaaagttt cttcatgatt gatatcactt gtcataatat
     1501 accattgaat cgtatgtcca gattgatgat tcaatgtttt taattggtta gcttgaagtt
     1561 caaataaact tacaccttca atttcaaacg aacctttagg gcccttgtaa ccaagtcttg
     1621 ttccttgacc acctgccatc aaaagtactg caaattgtcc ttctttaata gcttgaagtc
     1681 cttgttcctc taaacgctta atctcatcat cactcatttg tgatttaata tcatatttca
     1741 cttcagaaac tgcagataca tcatctattg ttttcttatt gatgtataaa tcgttatata
     1801 atttggcaat aaaatctaaa tctaatgacg ctaccttctc ttcaagtgct tctttttcat
     1861 tgttactcat tattttttca tattcacaca aatgatcttg tttatattta gctaattgat
     1921 ttttatctag cattttatgc ctcctgctga gtttgtaacc tttaataatt tattttctat
     1981 aaaaacttag tattccagtt gcttattata tcattgatga aaggctgaaa taaaacataa
     2041 actgttccga ccattaacgc tgtagctaaa gataagtcta caagtccacc tgttttaaat
     2101 tgaatcggtg tcttcacatt aaacggtaac ggataaaata atttcacgcc ttttggtgtt
     2161 atcatatcta gtataacatg tgacactaat ccaattataa ttgctgccat ataataatta
     2221 ggcgtctgaa taatttgcaa taaaaatgct atgattgcga taaataatat tgaatgcgta
     2281 aaggttcgat gtccaaagat caatctcaca aaaaaactaa ttaccttaaa ccttctgcca
     2341 atcttacttt gagtgtgaca tatatcgggt aataagctag ctagagttgc tagaatgata
     2401 accgtaaccg acgaaaaaat atccgtttga aaatattgtg ttgttagcgc tccaacgagc
     2461 atgccgcatg aagcatgtgt tttacctgtc atatttgttc tcctttaata ctcacatttt
     2521 accacatccc taacaaaaac acgaacatat tttcgggtta aaattcatta gtatgacaca
     2581 atttaaaaaa gtatcacata actcttgaaa acgattacaa aatcgtttat gatgtattta
     2641 caaaatattt aaaggatgtg tttgaataat ggcaatgaca gtaaaaaagg ataataatga
     2701 agtgcgtatt caatggagag ttgctgatat caaaattcct acaagtgaaa ttaaaaatat
     2761 tacacaagac caagatattc atgcagttcc taaattagac agcaaagatg tatctagaat
     2821 cggctcaacg tttggtaaaa cgaatcgcgt tattatcgat actgaagacc acgaatacat
     2881 tatttatact caaaatgatc aaaaagttta caatgaatta actaaataaa ttgtataaaa
     2941 aaatcattca tggtgagggc ttcatgaatg atttttttaa ttgattcaac acccagcata
     3001 aacaaataca aaaggacaac tgttcccata attttaacag ttgtcctttt tcacatatat
     3061 ttataacaaa agatgtgcca tcaaagaaat aattggtagt gtaatgattg ttctaatcaa
     3121 gaaaatcata aacaatttgc cgatgcttac aggaatcttc gaaccaagta tgacgccacc
     3181 tacttcagac aagtatatta actgcgatat actaagtgcc ccaataacaa aacgagttat
     3241 atcattttgt acaccttcaa ttaatataga aggtaaaaac atatcggcaa aaccgataat
     3301 aatcgtttga gaagcctgtg ccgcttcagg tatttgcatt aactctaaaa atggaacaaa
     3361 aggtttaccc aatatgacaa aaaagggcgt gtagttcgca ataatggtag caatagtacc
     3421 aatactcatt actacaggca aaataacaaa ccacatatca atgactgttt ttaatcctga
     3481 cttaaaaaag tcaataacgc ccggtgcttt aatacctact tctgttgcag tatcaaagcc
     3541 atgtctcaat gccgtctttc cttctggcaa tgcctcagta cgcgcacttt caggtacctc
     3601 cttagcatac tcatcaggaa ttttatttaa aggccaaatt cttggcataa tgactgctgc
     3661 aacgaggcag gatactatca ctgataaata gaaagcaaaa aattgatttt gcatgtgcac
     3721 tgtttcagca actacaattg caaaggtgat agaaactaca ctaaatgtcg ttgaaataac
     3781 tgttgcctca cgacgagaat aatatccttc accatattgt ctacttgtaa ttaagacacc
     3841 aacagttccg tctccaataa atgatgctaa attatctacc gtcgaacgtc ctggcaatgt
     3901 aaataaaggt ctcataaccg gtctaaaaat aggacccaac atctctaaca aaccgtattc
     3961 cattaatagc ggtaaaaata gagctgcaaa taagaatact gccactaatg ttggcaataa
     4021 acttgagaac aataatccac ccgtttcatc tgagtaaata acctttgaac caattcgtaa
     4081 aaatgtcatc catgcaaaaa caactgctaa tattcgtaaa attaaccaac caattctaac
     4141 gttaaaagca ttgttcatta gcccgtcagg tttcaattta tcttttaaaa tagttgaaca
     4201 aatcagagtt atgatacccg ataaagtaat tatcgtcaca attaaaaatg gcattacgcc
     4261 acctaataca tctttaagca cgcctgctaa aaatgccacg ggcaacgttg tttgcttctg
     4321 tccatcttgt tcgactggaa ttggtactaa aaataataag ataccaatta aagacatcgt
     4381 aataaactta agtctcccaa taactatctc tttccttgaa aagctatcca taaaatcaat
     4441 ccatttctct atgtattcgt tttaagt
//
LOCUS       DAKTIP010000191         4462 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.194, whole genome shotgun sequence.
ACCESSION   DAKTIP010000191 DAKTIP010000000
VERSION     DAKTIP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.194"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            62..3832
                     /locus_tag="O0305_002364"
     CDS             62..3832
                     /locus_tag="O0305_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCY0275475.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(3902..4012)
                     /locus_tag="O0305_002365"
     CDS             complement(3902..4012)
                     /locus_tag="O0305_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275476.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(4039..4458)
                     /locus_tag="O0305_002366"
     CDS             complement(4039..4458)
                     /locus_tag="O0305_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0275477.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDIKSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
BASE COUNT         1671 a          809 c          731 g         1251 t
ORIGIN      
        1 tatataaaac aggtactata atgttagtaa taattaataa atgttaggag taataaatag
       61 aatggcgaaa aaattcaatt acaaactacc atcaatggtt gcattaacgc ttgtaggttc
      121 agcagtcact gcacatcaag ttcaagcagc tgagacgaca caagatcaaa ctactaataa
      181 aaacgtttta gatagtaata aagttaaagc aactactgaa caagcaaaag ctgaggtaaa
      241 aaatccaacg caaaacattt ctggcactca agtatatcaa gaccctgcta ttgtccaacc
      301 aaaaacagca aataacaaaa caggcaatgc tcaagtaagt caaaaagttg atactgcaca
      361 agtaaatggt gacactcgtg ctaatcaatc agcgactaca aataatacgc agcctgttgc
      421 aaagtcaaca agcactacag cacctaaaac taacactaat gttacaaatg ctggttatag
      481 tttagttgat gatgaagatg ataattcaga aaatcaaatt aatccagaat taattaaatc
      541 agctgctaaa cctgcagctc ttgaaacgca atataaaacc gcagcaccta aagctgcaac
      601 tacatcagca cctaaagcta aaactgaagc gacacctaaa gtaactactt ttagcgcttc
      661 agcacaacca agatcagttg ctgcaacacc aaaaacgagt ttgccaaaat ataaaccaca
      721 agtaaactct tcaattaacg attacattcg taaaaataac ttaaaagcac ctaaaattga
      781 agaagattat acatcttact tccctaaata cgcataccgt aacggcgtag gtcgtcctga
      841 aggtatcgta gttcatgata cagctaatga tcgttcgacg ataaatggtg aaattagtta
      901 tatgaaaaat aactatcaaa acgcattcgt acatgcattt gttgatgggg atcgtataat
      961 cgaaacagca ccaacggatt acttatcttg gggtgtcggt gcagtcggta accctagatt
     1021 catcaatgtt gaaatcgtac acacacacga ctatgcttca tttgcacgtt caatgaataa
     1081 ctatgctgac tatgcagcta cacaattaca atattatggt ttaaaaccag acagtgctga
     1141 gtatgatgga aatggtacag tatggactca ctacgctgta agtaaatatt taggtggtac
     1201 tgaccatgcc gatccacatg gatatttaag aagtcataat tatagttatg atcaattata
     1261 tgacttaatt aatgaaaaat atttaataaa aatgggtaaa gtggcgccat ggggtacgca
     1321 atctacaact acccctacta caccatcaaa accaacaaca ccgtcgaaac catcaactgg
     1381 taaattaaca gttgctgcaa acaatggtgt cgcacaaatc aaaccaacaa atagtggttt
     1441 atatactact gtatacgaca aaactggtaa agcaactaat gaagttcaaa aaacatttgc
     1501 tgtatctaaa acagctacat taggtaatca aaaattctat cttgttcaag attacaattc
     1561 tggtaataaa tttggttggg ttaaagaagg cgatgtggtt tacaacacag ctaaatcacc
     1621 tgtaaatgta aatcaatcat attcaatcaa acctggtacg aaactttata cagtaccttg
     1681 gggtacatct aaacaagttg ctggtagtgt gtctggctct ggaaaccaaa catttaaggc
     1741 ttcaaagcaa caacaaattg ataaatcaat ttatttatat ggctctgtga atggtaaatc
     1801 tggttgggta agtaaagcat atttagttga tactgctaaa cctacgccta caccaacacc
     1861 taagccatca acacctacaa caaataataa attaacagtt tcatcattaa acggtgttgc
     1921 tcaaattaat gctaaaaaca atggcttatt cactacagtt tatgacaaaa ctggtaagcc
     1981 aacgaaagaa gttcaaaaaa catttgctgt aacaaaagaa gcaagtttag gtggaaacaa
     2041 attctactta gttaaagatt acaatagtcc aactttaatt ggttgggtta aacaaggtga
     2101 cgttatttat aacaatgcaa aatcacctgt aaatgtaatg caaacatata cagtaaaacc
     2161 aggcactaaa ttatattcag taccttgggg cacttataaa caagaagctg gtgcagtttc
     2221 tggtacaggt aaccaaactt ttaaagcgac taagcaacaa caaattgata aatctatcta
     2281 tttatttgga actgtaaatg gtaaatctgg ttgggtaagt aaagcatatt tagctgtacc
     2341 tgctgcacct aaaaaagcag tagcacaacc aaaaacagct gtaaaagctt atactgttac
     2401 taaaccacaa acgactcaaa cagttagcaa gattgctcaa gttaaaccaa acaacactgg
     2461 tattcgtgct tctgtttatg aaaaaacagc gaaaaacggt gcgaaatatg cagaccgtac
     2521 gttctatgta acaaaagagc gtgctcatgg taatgaaacg tatgtattat taaacaatac
     2581 aagccataac atcccattag gttggttcaa tgtaaaagac ttaaatgttc aaaacttagg
     2641 caaagaagtt aaaacgactc aaaaatatac tgttaataaa tcaaataacg gcttatcaat
     2701 ggttccttgg ggtactaaaa accaagtcat tttaacaggc aataacattg ctcaaggtac
     2761 atttaatgca acgaaacaag tatctgtagg caaagatgtt tatttatacg gtactattaa
     2821 taaccgcact ggttgggtaa atgcaaaaga tttaactgca ccaaccgctg tgaaaccaac
     2881 tacatcagct gccaaagatt ataactacac ttatgtaatt aaaaatggta atggttatta
     2941 ctatgtaaca ccaaattctg atacagctaa atactcatta aaagcattta atgaacaacc
     3001 attcgcagtt gttaaagaac aagtcattaa tggacaaact tggtactatg gtaaattatc
     3061 taacggtaaa ttagcatgga ttaaatcaac tgatttagct aaagaattaa ttaagtataa
     3121 tcaaacaggt atgacattaa accaagttgc tcaaatacaa gctggtttac aatataaacc
     3181 acaagtacaa cgtgtaccag gtaagtggac agatgctaac tttaatgatg ttaagcatgc
     3241 aatggatacg aagcgtttag ctcaagatcc agcattaaaa tatcaattct tacgcttaga
     3301 ccaaccacaa aatatttcta ttgataaaat taatcaattc ttaaaaggta aaggtgtatt
     3361 agaaaaccaa ggtgctgcat ttaacaaagc tgctcaaatg tatggcatta atgaagttta
     3421 tcttatctca catgccctat tagaaacagg taacggtact tctcaattag cgaaaggtgc
     3481 agatgtagtg aacaacaaag ttgtaactaa ctcaaacacg aaataccata acgtatttgg
     3541 tattgctgca tatgataacg atcctttacg tgaaggtatt aaatatgcta aacaagctgg
     3601 ttgggacaca gtatcaaaag caatcgttgg tggtgctaaa ttcatcggca actcatatgt
     3661 aaaagctggt caaaatacac tttacaaaat gagatggaat cctgcacatc caggaacaca
     3721 ccaatatgct acagatgtag attgggctaa catcaatgct aaaatcatca aaggctacta
     3781 tgataaaatt ggcgaagtcg gcaaatactt cgacatccca caatataaat aagcaacatg
     3841 aacataggat caaaagtcat cccccactat caatcatggg ggatgacctt tgatcccttt
     3901 tttatacata cacaagcaaa aatagcggtg attgtttacc atcaatttta acaatcaccg
     3961 ctacttttgc ttgtaattca tgattcaatt tttgttgtgt gcacaacgac actaaattat
     4021 gtgtttgcta ttgtcgtgtt acaacgatat gcgtcgttga tttaacttat caagtaattg
     4081 atttaaattg tctaattcga cttccgataa acattgacat cttgcttcaa tcaattcgca
     4141 acgtgcatta tttatttgtg aaattaatgt acgtgcttga tcagtcaaaa ttaattcttt
     4201 acatcttaaa tcttcgctag attgttgact tgtgatgtac cctttcaaaa ctaatttctt
     4261 aatccacctt gaaactatag attgttccct gctaatcttc atcgtcaaat catactgtga
     4321 taagcgatcg taattaaata aaatacggag aagttctagc tgttcagatt tgatatccgt
     4381 gcgttgtcca aatcttctat tcactaagtt taagtcattg ttagtcagtg taataagttt
     4441 ttcaagttgt ttatacatgt tg
//
LOCUS       DAKTIP010000192         4405 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAKTIP010000192 DAKTIP010000000
VERSION     DAKTIP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.195"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            163..432
                     /gene="rpsN"
                     /locus_tag="O0305_002367"
     CDS             163..432
                     /gene="rpsN"
                     /locus_tag="O0305_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCY0275478.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            589..1566
                     /gene="guaC"
                     /locus_tag="O0305_002368"
     CDS             589..1566
                     /gene="guaC"
                     /locus_tag="O0305_002368"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCY0275479.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKTHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1588..2604
                     /locus_tag="O0305_002369"
     CDS             1588..2604
                     /locus_tag="O0305_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCY0275480.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYMKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2984..3607)
                     /gene="lexA"
                     /locus_tag="O0305_002370"
     CDS             complement(2984..3607)
                     /gene="lexA"
                     /locus_tag="O0305_002370"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCY0275481.1"
                     /translation="MRELTKRQSEIYNYIKQVVQTKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            3750..3983
                     /locus_tag="O0305_002371"
     CDS             3750..3983
                     /locus_tag="O0305_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275482.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            4119..4358
                     /locus_tag="O0305_002372"
     CDS             4119..4358
                     /locus_tag="O0305_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCY0275483.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
BASE COUNT         1681 a          584 c          748 g         1392 t
ORIGIN      
        1 ggtatgtttt ataaatgttc gataatacac tttaatctta aatatgatgg tttagaaaat
       61 gatttaacaa agaaatgaaa ctttactgtt gaattatgtg aggattgtgt tattatataa
      121 atcgtaataa ttacgatttg ataaaaagtg aggtaactat atatggctaa gaaatctaaa
      181 atagcaaaag agagaaaaag agaagagtta gtaaataaat attacgaatt acgtaaagag
      241 ttaaaagcaa aaggtgatta cgaagcgtta agaaaattac caagagattc atcacctaca
      301 cgtttaacta gaagatgtaa agtaactgga agacctagag gtgtattacg taaatttgaa
      361 atgtctcgta ttgcgtttag agaacatgcg cacaaaggac aaattccagg tgttaaaaaa
      421 tcaagttggt aaaatcaaat ttcgtacttt agcccattta caatatcaat aaaacaatgt
      481 acagtatata cgaatgctat aaactgaatg ttttctcata ttaataagga aacattcggt
      541 ttttaatttg catttaaaaa taattagttt ttaaaggggc tatttaaagt gaaaatattt
      601 gattacgaag atattcaatt aatacctaat aaatgcatag ttgaaagtag gtctgaatgt
      661 gatacaacta tccaatttgg tccgaaaaaa ttcaagctac ctgtagttcc tgcaaatatg
      721 caaacagtta tgaatgagaa attagcgaaa tggtttgctg aaaatgatta cttttatatc
      781 atgcatcgtt ttgatgaaga agcaagaata ccttttataa aacatatgca aaattcaggc
      841 ttatttgcat ctatttcagt tggtgtaaag aaagcggaat ttgattttat tgaaaagtta
      901 gctcaagaaa aattaatccc cgaatatatt acaatagata ttgcgcatgg tcactcagat
      961 tcagtgataa acatgattaa acatataaaa acccatatac ctgatagttt tgttattgct
     1021 ggtaatgttg gtacgccaga aggtgttaga gaattagaaa atgctggtgc tgatgctacc
     1081 aaagtcggta taggtcctgg tagagtttgt attacaaaga ttaaaacagg ttttggtact
     1141 ggtggttggc agttagcggc attaaacata tgtagtaaag cagctcgtaa acctttgatt
     1201 gccgatggtg gtataagaac gcatggcgac attgctaaat caattagatt tggtgcatca
     1261 atggtcatga ttggttcatt atttgcggca cacgaagaat cacctggtga aactgtagaa
     1321 cttgatggta aacagtataa agaatatttt ggtagtgcat ctgaatttca aaaaggcgaa
     1381 cataaaaatg tagaaggtaa aaaaatgttt gtagaacata agggttcatt aatggatacc
     1441 ttaaaagaaa tgcaacaaga tttacaaagc tcaatttcat atgccggtgg aaaagacttg
     1501 aaatcattac gtactgtaga ttatgttatt gttagaaact ctattttcaa cggtgataga
     1561 gattaatatt tatagtaggt gatgtaaatt aaaaaattca tagtaactgt tgttgcgttt
     1621 ttatcaatta ttatcattgc gccaataaca gaatttaaac cattcattca tttacaaaat
     1681 gaagtaagac aatatattga cattcacatc aataaagaaa caatttctgc ggaaaataaa
     1741 ttggatacac cgaagaaaca acaatttgcc tttaataata tacaaatgaa catgtcgaaa
     1801 tcagatgttg agaaaacatt aaataaacca aaaagagtga catttaatga atatggtacg
     1861 aagtggtata cgtattatga tgacgattac aataatttta taatgataag ttacatgaaa
     1921 gataaagtta atgcgttata tacaaatcaa aatataatca cttcaaaatc aaaaattaaa
     1981 tataatacac ctaaatcggt tgtaaggcaa agattaggcg aaccagaaac agagattgtt
     2041 aaaggtagag tgcgttacga acaaaataat aaagaatatg atgttttcca taaaaatcac
     2101 atttatacga cggtatttta tgataagcat cgacgtaata atgtaacagc tgttttacaa
     2161 gtaagtgatg ctatggaaaa tagattaaaa gaacaatatg gagcaccatc gaaatcgctt
     2221 gcagatagtt ttgaactaca aaattttgat ttagttaatg ctgaaagaaa acaacatcaa
     2281 ttatctacat tgaagtattc taaacagaat tctgaaactg cacgtaagca tagtaaagat
     2341 atggccaaca atcattattt tgatcataca aatttaaaag gtcaatcacc atttgatcga
     2401 ttgaaaaaag atggtattac atttaactca gccggagaga atttagcata tggtcaagtt
     2461 agtagtatct atgcacatca aggattaatg aattctattg gtcacagaaa aaatatttta
     2521 aatgatacgt ttaaaatatt aggtgttggt gttgatttta atgatgaaaa acaacctttt
     2581 tggacagaaa attatactgg ttaatgtttt tggcatgaga ttaaagggta atgtttgtct
     2641 gtaaggaagt agataaacat gatgacaaca gataaaccaa aagatgcgga tatacttgaa
     2701 cgcgtcaaag atatacttaa taaaaaagaa agaaaaaaat aataatggct cgctcctgta
     2761 aattattacg ggggcgagtt ttaatttgtg taacattttg cagaaatctg taattcaaaa
     2821 tttatattaa agaatgagat tatctaacaa ttcgcaattt ctgtttgaac tgaatttaga
     2881 ttagaataaa taaaacctct tgaaaacgta actttttcaa gaggtccaaa atttataaaa
     2941 atgagggctt aacaattacg ataatatata acggtttaaa atattacatt tcgcggtaca
     3001 aaccaattac tttcccaatt acagcaacat tgtctaggta aattggctcc attgtactat
     3061 tttcaggttg taatcgataa cgattttttt ctttatagaa gcgtttgaca gttgcttcat
     3121 cttcctcagt catagcaaca ataatgtctc cattttctgc tatggtttga ctgcgaacaa
     3181 ttactttgtc tccgtctaat ataccagcct caatcatact gtcgcctacg acgtttaata
     3241 tgaatatgtc gctattgtgt gtcgatgtta agtgttcagg taatggaaaa tattcttcaa
     3301 tattttctac tgcggtaata ggaacacctg ctgtgacttt accaataact ggcacatgaa
     3361 tcgtttcttc catattaata ttatcatttg tttgatcact tacaatttct atagcacgtg
     3421 gtttcgttgg atctcttctt atatagcctt tttcttcaag acgtgaaagg tgaccatgaa
     3481 cagttgaact ggatgctaag ccaactgctt caccaatttc gcgaacacta ggcggataac
     3541 ctttcgtttg aacaacttgt ttaatatagt tatatatttc gctttgtcgt tttgttaatt
     3601 ctctcatata taggcactcc ctaaattaat ttaaatacag tatatcatta atttgaaacc
     3661 aaaacaaaca tttgttcgtt aaaatattga cacagaacat aagttctgat aaattactaa
     3721 tacaaacaaa tgttctagga gtgaaaatga tgttttacaa taaatataaa aacgtatcaa
     3781 catatatcat catattttta gtttcaagtg cagcctttgc aatattcttg ttaagtgcga
     3841 acattagtgc tcactcggaa caagtgtacg aaatgactga ccatcaaatt aagaacaata
     3901 cgataaataa agcatacgaa cataaagacc ctacaaacaa tagcgaacaa agagatggga
     3961 aagtgttcgc tttaataaat tgatacattg tcacaacgtt attttgccta tttttgcgaa
     4021 atagcgtttt ttattacttt tttgctgatc ttaaatttgt tatattttgt taaagtatta
     4081 taatgattga ataaacaaat tgaaggtagg ttttttaatt gagtaattct gatttgaata
     4141 tcgaaagaat taacgagtta gctaaaaaga aaaaagaagt aggattaact caagaagaag
     4201 caaaggagca aacagcctta agaaaagctt atcttgagag ttttagaaaa gggtttaaac
     4261 aacaaattga aaatactaaa gtaattgatc cagaaggtaa tgatgtaaca cctgaaaaaa
     4321 ttaaagagat acaacaaaaa agagataata aaaattaaat cacaaatctg taaagaattt
     4381 tctgacatta taacttgaaa taagt
//
LOCUS       DAKTIP010000193         4364 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.196, whole genome shotgun sequence.
ACCESSION   DAKTIP010000193 DAKTIP010000000
VERSION     DAKTIP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.196"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            24..2012
                     /gene="tkt"
                     /locus_tag="O0305_002373"
     CDS             24..2012
                     /gene="tkt"
                     /locus_tag="O0305_002373"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCY0275484.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            2290..2532
                     /locus_tag="O0305_002374"
     CDS             2290..2532
                     /locus_tag="O0305_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCY0275485.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            2519..2617
                     /locus_tag="O0305_002375"
     CDS             2519..2617
                     /locus_tag="O0305_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275486.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            2711..3178
                     /locus_tag="O0305_002376"
     CDS             2711..3178
                     /locus_tag="O0305_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCY0275487.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            3302..>4364
                     /locus_tag="O0305_002377"
     CDS             3302..>4364
                     /locus_tag="O0305_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCY0275488.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELS"
BASE COUNT         1555 a          600 c          834 g         1375 t
ORIGIN      
        1 ttgaagaagg gaagatacac aagatgttta atgaaaaaga tcaattagct gttgatacgc
       61 tacgtgcact aagtatcgac acaatcgaaa aagcgaattc tggtcatcca ggattaccta
      121 tgggagctgc cccaatggct tacactttgt ggacacgtca tctgaatttt aatccacaat
      181 ctaaagatta cttcaataga gaccgtttcg tattatctgc agggcatggt tcagcattat
      241 tgtatagctt gttacatgtt tctggtagtt tagaattaga agaattaaag caatttagac
      301 aatggggttc taaaacacca ggtcatcctg aatacagaca tacagatggt gtagaagtta
      361 ctaccggacc acttggacaa ggttttgcta tgtcagtagg attagcttta gcagaagatc
      421 acctagcagg gaaatttaat aaagaaggat ataatgttgt agatcattac acatatgtat
      481 tagcttctga cggtgattta atggaaggta tatcgcatga agcagcttca tttgctggac
      541 ataataaatt aagtaaatta gttgttttat acgattcaaa tgatatttca ttagatggcg
      601 aattaaacaa agctttttct gaaaacacaa aagctcgttt tgaagcatat ggttggaatt
      661 acttactagt taaagatggt aatgatttag aagaaattga taaagcgatt actacagcta
      721 aatctcaaga aggaccaacg attattgaag ttaaaacaac aatcggattt ggttcaccga
      781 ataaagcagg aactaatggt gttcatgggg cacctttagg tgaagttgaa agaaaattaa
      841 cattcgaaaa ttacggttta gatcctgaaa aacgttttaa tgtttcagaa gaggtatacg
      901 aaattttcca aaatactatg ttaaaacgtg ctaatgaaga tgaatctcaa tggaattcat
      961 tattagaaaa atatgcagaa acatatcctg aattagcaga agaatttaaa ttagcgatta
     1021 gtggtaaatt gcctaaaaat tataaggatg aattaccacg ttttgaactg ggtcataatg
     1081 gtgcatctcg tgctgattct ggtactgtta ttcaagcaat cagtaaaact gtcccttcat
     1141 tctttggtgg atcagcagac cttgctggtt caaacaaatc caatgtaaat gatgcaactg
     1201 attatagttc tgaaacacct gaaggtaaaa atgtgtggtt tggtgtacgt gaatttgcta
     1261 tgggtgctgc tgtaaatggt atggctgcac atggaggttt acatccatat ggtgcaacat
     1321 tcttcgtatt tagtgattat ttaaaaccag cgttacgttt atcatcaatt atgggattaa
     1381 atgcaacgtt catcttcaca catgattcaa ttgcagtagg tgaagatggt cctactcatg
     1441 aaccaattga gcaattagct ggattaagag ccattccaaa tatgaatgtt atccgtcctg
     1501 ctgatggtaa tgaaacaaga gtagcatggg aagttgcctt agaatctgaa tctacaccta
     1561 cttcattagt attgacacgt caaaacttac cggtattaga tgtaccagaa gatgtagttg
     1621 aagaaggcgt tcgaaaaggt gcctatacag tttatggctc tgaagagaca ccagaattcc
     1681 tattattagc ttcaggttca gaagttagtc ttgcagttga agctgctaaa gatcttgaaa
     1741 aacaaggtaa atcagtacgt gttgtttcaa tgcctaactg gaatgcattt gaacaacaat
     1801 ctgaagaata taaagaatca gttattccat caagcgtaac aaaacgtgtt gcgattgaaa
     1861 tggcttcacc gcttggatgg cataaatatg taggtactgc aggtaaagtt attgctattg
     1921 acggctttgg cgcaagtgca cctggcgatt tagtagttga aaaatatgga tttacaaaag
     1981 aaaatatctt aaaccaagtt atgagcttat aagaataatt tataaagcga gtatgtttag
     2041 aagtctagga tgcataatct taggcttctt ttaaagtgtt gaaatttaga gtatagcact
     2101 taaactacat cataagtgat aagttatgaa agtatactat ttcagattaa tctttaaaag
     2161 ctctgttata acagcatgat ttttgatatt atttttagta tcgatattaa aatacttgaa
     2221 taaactagtt cttgaaataa tgtgatgaat ttagtaaaat tcagtaggat aaagaaagcg
     2281 ggtgaaacaa tggcaacttg gttagcaatt atttttatag tagctgcatt aattttaggt
     2341 ttaattggag gtttcctttt agctagaaaa tatatgatgg actacttgaa gaaaaaccca
     2401 ccaatcaacg aagaaatgct tcgtatgatg atgatgcaaa tgggtcaaaa accttctcag
     2461 aagaaaatta atcaaatgat gacgatgatg aataaaaata tggatcaaaa tatgaagagt
     2521 gcgaaaaagt aaattcgcaa ttgatagagg ctattttcca gatatggaaa tggcctcttt
     2581 ttataatcaa attaataaga ataaatatgt ttattaaaat taaagttaac aaaatgacga
     2641 atagactgag aaatgctata attcattttg tatgatttac agagagttta tttaacgaga
     2701 aggtgtctgc gtgctctatt taatattttc aatcattgta gctttattta tgggaactat
     2761 agttatagtt attcgtatga aagctcaaaa ttatccggta aatgagaaaa aaatagtttt
     2821 gccaccgttt tttatggcga ccggtgcatt gatgtacgtc gttccatatt ttaggctaac
     2881 aggatcggaa atgctagaag cctttataat tggtttgctt ttttctacag ttctaatttg
     2941 gacttctcga tttgaagtca aaggtacaga aatttatatg aaacgatcta aagcatttcc
     3001 agttattttg atttcattac ttatcattcg tactgtgatg aaaatattca ttagtaatga
     3061 aatagatcct ggagaattag gcggcatgtt ctttttatta gcattctgta tgattgttcc
     3121 ttggagagca gcaatgctat ataaatacaa aaaactaaag aaaacattaa tcaattaatt
     3181 acttttaaaa ccacttgtga tcgacttcta aatcagtcaa tgagtggttt taattttact
     3241 tgaaaaggtg aaatgattat attaaaataa aagcgaacaa atgttctata tggaggcagt
     3301 aatgaaaatt atacatacag cagactggca cttagggaaa atattaaatg gcaaacagct
     3361 tttagaagat caagcgtata ttttagatat gttcgtagaa aaaatgaaag aagaagaacc
     3421 tgatatcatt gtgatagctg gagatttata tgacacaaca tatccaagta aagatgcaat
     3481 catgttatta gaacaagcga ttggaaagtt aaatttagaa ctgcgtatac caataattat
     3541 tattagtgga aatcacgatg gtaaagagag gttaaactat ggggcgagtt ggtttgaaca
     3601 taatcagtta tttataagaa cagattttac atcgattaat tcaccaatag agataaatgg
     3661 ggttaatttt tatacactcc cttatgctac tgtgagcgaa atgaaacact actttgaaga
     3721 tgacaccatt gaaacacatc aacagggaat tacgcgctgt attgaaacaa tagcaccgga
     3781 aattgatgaa gatgccgtca atattttaat tagtcatctg actgttcaag gtggaaagac
     3841 atctgattct gaaagaccat taactattgg aacggttgaa tcagttcaga aaggtgtttt
     3901 tgatatattt gattatgtca tgctaggtca cttgcatcat ccatttagta tagaagacga
     3961 caaaattaaa tatagtggct ccttattgca gtattcattt tcggaagcgg gtcaagctaa
     4021 agggtataga cgtgtaacaa ttaatgatgg cattattaac gatgtattta ttcctcttaa
     4081 gccacttaga caattggaaa ttatctcagg cgaatataat gatgttatta atgaaaaagt
     4141 tcatgtgaaa aataaagata attatttaca ttttaaactt aaaaatatgt ctcatattac
     4201 tgatccaatg atgagtttaa aacaaattta tcctaatact ttagcgctga cgaatgaaac
     4261 ttttaattac aatgaagaaa ataatgctat agaaataagt gaaaaagatg acatgtcaat
     4321 tatcgaaatg ttttataaac acataactga taaagaatta tcgg
//
LOCUS       DAKTIP010000194         4363 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAKTIP010000194 DAKTIP010000000
VERSION     DAKTIP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.197"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            473..1900
                     /gene="treP"
                     /locus_tag="O0305_002378"
     CDS             473..1900
                     /gene="treP"
                     /locus_tag="O0305_002378"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCY0275489.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            1964..3604
                     /gene="treC"
                     /locus_tag="O0305_002379"
     CDS             1964..3604
                     /gene="treC"
                     /locus_tag="O0305_002379"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCY0275490.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWYSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA"
     gene            3629..4357
                     /gene="treR"
                     /locus_tag="O0305_002380"
     CDS             3629..4357
                     /gene="treR"
                     /locus_tag="O0305_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCY0275491.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRIK"
BASE COUNT         1389 a          589 c          964 g         1421 t
ORIGIN      
        1 catcaatatt tatattaaat tctatatata atgaaggtaa gtgctcaaat tttgagtatt
       61 tacctttttt atttgtcttt gaatggctcg taatttttga taatagaaat gataaggcat
      121 tgagattgga agggcatttg gcttgtgcaa tatacatagc taaatgtctt ttttgttttg
      181 tgaaatatga tggatggctt gtgtggacaa gtttgctatt tatagatatg catttttcaa
      241 tttaggagtt ggccatgcat ctacacttta taatggtgag agcgtggtga ggtattgtta
      301 ataacgcaat tgtagcgagg agttattgct acatatgtcg ttatggctca ttgattttct
      361 gaaatggcta ccccagataa ttgtgacaaa ataaaaatat tttgttgaaa gcctttacat
      421 aacttgtcta gacaagttat actcgtttta agacattaag ggagtgaaat atatggctgt
      481 aaaaagagaa gatgtaaaag ccatcgtaac cgctattggg ggaaaagaaa atcttgaagc
      541 tgcaacgcat tgtgtaacac gattacgttt agtgctgaag gatgaaagta aagttgataa
      601 agacgcatta agtaataacg cgttggtcaa ggggcagttt aaagcagacc atcaatatca
      661 aattgtcatt ggtccaggaa cagtcgatga agtgtataag cagtttattg atgaaacagg
      721 tgctcaagaa gcttcgaaag atgaagcgaa acaagcagct gcacaaaaag ggaatccagt
      781 acaacgtttg atcaaattgt tgggggatat ttttatacca atattacctg cgattgtgac
      841 agctggtttg ttaatgggaa tcaataattt acttacaatg aaaggtttat ttggtccaaa
      901 agcacttatt gagatgtatc cacaaattgc tgatatttca aacatcatta atgtgattgc
      961 gagtacggca tttattttct taccagcatt aattggttgg agtagtatgc gtgtatttgg
     1021 tggtagtccg attctaggca tagtcttagg tttgatttta atgcatccgc aattagtatc
     1081 tcagtatgat ttggcaaaag ggaatattcc gacgtggaac ttatttggct tagagattaa
     1141 gcagttgaat taccaaggtc aagtgttgcc agttttaatt gcagcttacg ttctagctaa
     1201 aattgaaaaa ggattaaata aagtcgttca cgattcgata aaaatgttgg tcgttggacc
     1261 cgtagcgctt ttagttactg gatttttagc atttattatc attggaccag ttgcgttatt
     1321 gattggtaca ggtattacat ctggtgttac atttatattc caacatgcag gatggcttgg
     1381 cggagcaata tatggattgt tatatgcacc acttgtaatt acaggactac accatatgtt
     1441 tttagcagta gatttccaat tgatgggtag cagcttaggc ggtacgtatt tatggccaat
     1501 tgttgcgatt tccaatattt gtcagggctc tgcagcattt ggagcatggt ttgtctataa
     1561 acgtcgtaaa atggttaaag aagaaggctt ggcattaaca tcttgtattt ctggtatgtt
     1621 aggtgttact gaaccagcca tgttcggtgt gaacttacct ctgaaatatc catttatcgc
     1681 tgcgatatca acgtcttgtg tattgggggc aatcgttggt atgaataacg tacttggaaa
     1741 agttggtgtt ggtggcgtgc cagcattcat ttcaattcaa aaagaatttt ggccagtata
     1801 tcttattgtg acagctattg ctattgttgt accatgtata ctaacaattg tgatgtctca
     1861 ttttagtaaa caaaaagcga aagaaattgt tgaagattaa taaaataaaa aaggggcgtt
     1921 cgttatttgg acgtccttta ttacgttata aggtggtaat tgtgtgtcga aagaaataga
     1981 ttggagaaaa tccgttgtat atcaaattta tcctaagtcg tttaatgata cgacggggaa
     2041 tggtatagga gatatcaatg gaattataga aaaattggat tatatcaagt tattgggtgt
     2101 tgattatatt tggttaacac cagtgtatga atcaccgatg aatgataatg gctatgatat
     2161 cagcaattat ttagaaatca atgaagactt tggaacgatg gatgattttg aaaagttaat
     2221 caaagttgcg catcaaaaag acttgaaagt gatgttagat attgtcatta atcatacgtc
     2281 gacggagcat gaatggttta aagaagcccg taaatctaaa gataaccctt atagagatta
     2341 ttactttttc agatcatctg aagacgggcc gccaacaaat tggtattcta aattcggtgg
     2401 taatgcatgg aagtatgatt ctgagacaga tgaatattat ttacatttat ttgatgtcag
     2461 tcaagctgat ttaaattggg ataatccgga agtacgtcaa tcgttatatc gcatagtcaa
     2521 tcattggata gacttcggcg ttgatggttt tcgatttgat gtcattaact taatttctaa
     2581 aggtgaattt aaggactctg acaaaatagg taaagaattt tatacggatg gtcctagagt
     2641 gcatgagttt ctgcatgaat taaatcgtca aacgtttggt aacactgaca tgatgactat
     2701 aggagaaatg tcttcgacga cgattgaaaa ttgtattaag tatacacaac cagaacgcca
     2761 agaattgaat agtgttttta attttcatca tctaaaggtt gattatgttg atggtgaaaa
     2821 gtggacaaat gcgaagcttg attttcataa gttaaaggaa attctgatgc aatggcaacg
     2881 aggtatttat gacggtggcg gatggaacgc gattttctgg tgtaatcatg atcagccacg
     2941 ggtagtgtct agatttggtg atgatacgtc ggaagagatg aggatacaaa gtgctaaaat
     3001 gttagctatc gcactgcata tgttgcaagg gacgccatat atttaccaag gtgaagaaat
     3061 tggtatgacg gacccacatt ttacatcaat agcacaatat cgtgatgttg aatcgattaa
     3121 tgcctaccat cagttgttaa gtgaagggca tgctgaagcg gatgtgttag cgattttagg
     3181 acagaagtca cgagacaatt cgagaacgcc tatgcaatgg agtgatgatg ttaatgctgg
     3241 atttacagct ggtaagcctt ggattgatat ttcggaaaat tatcatcagg tcaacgttag
     3301 acaagcactt cagaataaag agtctatttt ctatacgtat caaaaattaa tacaattaag
     3361 acatacgcat gatattatta cgtatggaga cattgtgcca cgttttatgg atcatgatca
     3421 tttatttgtt tatgaacgtc attataagaa tcaacaatgg ctagtaattg cgaatttctc
     3481 agcatcggct gttgatttgc cagaaggatt ggctagagaa ggttgtgttg tgattcaaac
     3541 aggcacagtg gaaaataata cgataagcgg gtttggtgca attgtaatcg aaacaaacgc
     3601 gtaaaataaa ttgagtggat gcgtttatat ggcgaaacaa aaaaagttta tgaagattta
     3661 tgaggcgttg aaagaagata tattaaacgg gcagattcaa tatggtgaac aaattccgtc
     3721 tgaacatgat ttggtgcaat tgtaccagtc atctcgagag accgtgcgta aggcattaga
     3781 tttgttggca ttagacggca tgattcaaaa gattcatggt aaagggtcac ttgtcattta
     3841 tcaggaggtt acagagtttc cattttctga acttgttagt tttaaagaaa tgcaagaaga
     3901 aatgggcgtc gcatatttaa ctgaagttgt tgtgaatgag gttgttgaag cgcatgaagt
     3961 tccagaagtt caacatgctt taaacatcaa ttctagtgaa tcactcattc atattgttag
     4021 aactcgtcgg cttaaccaac atgtgaagat tgttgatgaa gattattttc taaagtcgat
     4081 tgtttcagat ataggtaatg atgttgcgag tgattctatt tatgattatt tggaaaaggt
     4141 attaaatctt aatattagtt attcaagtaa gtctattact tttgaaccgt ttgatgaaca
     4201 agcatatcaa ttgtttggtg atgtatcggt ggcttattca gcaacagttc gaagtattgt
     4261 gtatttagaa aatacaatgc cgtttcaata taatatttca aaacatcttg caaatgaatt
     4321 taaatttaat gacttctcaa gacgtcgtat aaagtaaaca atg
//
LOCUS       DAKTIP010000195         4331 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAKTIP010000195 DAKTIP010000000
VERSION     DAKTIP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.198"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            230..571
                     /locus_tag="O0305_002381"
     CDS             230..571
                     /locus_tag="O0305_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCY0275492.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKVEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(623..1945)
                     /locus_tag="O0305_002382"
     CDS             complement(623..1945)
                     /locus_tag="O0305_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCY0275493.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSPKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAYGITDLENKGIAL
                     HTNVETTELSSDNHHTTVHTNVDNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(1932..2372)
                     /locus_tag="O0305_002383"
     CDS             complement(1932..2372)
                     /locus_tag="O0305_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCY0275494.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            2430..2531
                     /locus_tag="O0305_002384"
     CDS             2430..2531
                     /locus_tag="O0305_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275495.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            2997..>4331
                     /locus_tag="O0305_002385"
     CDS             2997..>4331
                     /locus_tag="O0305_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0275496.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSAGTAVGAGLATFVLGSNPVGWV
                     ILAGLAMSTVFALGTDLIYQNNIFGLKDKVDWVGHKIDNSIDVVKKTTEKSMD"
BASE COUNT         1525 a          629 c          800 g         1377 t
ORIGIN      
        1 ggacaaaaca tggtattaaa ccattaaaat ttaatattta tcatgggcaa taaatgattt
       61 gataggggag caatcatcta atttaattag aagataaatg ctccattttt tattgtcagc
      121 cgtgttacgt atcttgaaaa aaatattaaa aagagtaaaa tagatagagt taacgaaaaa
      181 attaatgaaa tcgacgatat agatatgata aagaaaggtg ggtagcaata tgaaaaatac
      241 attccttatt tgtgatgaat gtcaggcagt caatataaga acgttacaaa agaagttgga
      301 aaaattagat cccgatgctg aaatcgtgat aggttgtcaa tcttattgtg gacctggacg
      361 ccgaaaaaca ttcacttttg ttaataaccg cccactggct gcgcttactg aagaagaatt
      421 aatcgaaaaa gtttctcaac aattaaagaa accacgtgat cctgaagaag aagagcgttt
      481 aagaaaacga catgaagaac gtaaacgtcg taaagtagaa caagatagaa agcttaaaga
      541 aaaattagaa aagcgaaaag cacaacaata aagcctgatg gcagcatcat tcaatgcgtg
      601 ccaccaggtt tttatgtttt gtctagaaat taaataaatc attaaatgat tcggccatcg
      661 taggatgcgt ataaatatta tctcgtaata cggtatatgg aatgttttga tcaatcgcaa
      721 gtttaattat attaattaat tcttcagatt gcttaccata taatgtagca cctaaaatca
      781 tattattttc attattaatg actactttaa ataaacctct tggatcattg ttaattttat
      841 gacgaggtat agcacttact aaaagttgat gttcagtgta atcataatgt tgagcggcag
      901 cttctttact agttaatcca acacgtgata atggtggatc tataaatact gtataaggca
      961 cgctgcctct attgtcagtc gtacgtgact gattaccata taacgctgat ttgataattc
     1021 gataatcatc taaagatata tacgtaaatt gaagtccgcc tttaacatca cctgcagcat
     1081 aaatatgcgg cacagttgtt tgaagatgag cattgacttt aatttcgcct ctgtcgccta
     1141 attcgatatc agtattttct aaagctaaat ccgtattcgg tttgcgcccg atagccaaaa
     1201 gtactgcatc agcctcaaag ttatcaacgt tggtatggac tgttgtatga tgattgtcag
     1261 atgacaattc agtcgtttca acatttgtat gcaatgcaat gcctttattt tctaagtcag
     1321 taataccata tgcaacgaca tcttgatctt cgcgtggcat aaatgattcg ccacgttcta
     1381 atactgttac cttactacct aaattcgcaa acattgaagc aaattctaag gcgatataac
     1441 cgccacctac aataacgagg tgcttaggtt gatagctaat gtttaataaa cctgtcgaat
     1501 cgaagacgtg tttagcttga tcaaggcctt taatgttagg aatgacagag gtagcaccgg
     1561 tattaataat gatatgaggt gcagtaatac tatcgacgat atcgtcatgt tgatctaata
     1621 aattcacttc agtattagat ttaaactgcg ctttaaaatc cagtacatca atgttgttat
     1681 cgtctgctaa taagtggtaa tttttattgt ttagcgcatt gacaacatcg tttttacggt
     1741 tataacttgc ttcaaaagat ttgccttcta atccatcatg tacaagtgtc ttcgaaggta
     1801 tacatcctat gtttatacaa gtgcctccat acattttcgg agattgttcg ataactgcga
     1861 cgtgttgacc tgttgatgca gcgtatttcg ctaaagtttt accagctttc ccaaatccta
     1921 ttacaattaa atcatatgtt ttcatgacat aaatcctcct tttgaatgtc ttcaatgaca
     1981 tctttgattg tttttccatt ataaaaatta ataatgatat tctgttcgtc ttgctgataa
     2041 tgtgacatgg tagttgcaat attacgagca atttgacagt gactgccttc gtcgccagta
     2101 aatagacgtg tgtggtgttc tttctctaag acaaaatgtt tatataatgt tgctagagag
     2161 acatcagcac tttgatcatt tgctaaataa ccgccatctt tacctcgtat tgtgtcaatc
     2221 atttttaaat cgacaagttg agtcgtcacg cgtcgtaatt gaacaggatt taaacaagtt
     2281 aattctgcta atgaactact attgaatttt tctgaatgat gcttagttaa aaaagctaat
     2341 acatgcacgg caatgttaaa ttctaaattc aatgtttctc acctcataat tgtaactgta
     2401 ttagttacaa ttaaaaatca ttttgctcaa tgtgtcaata cttatgtttg aaaagtgttg
     2461 aattaggtgt ggttattgtg atgatttatt gtttgaaagt tgatgcatta acggtgagat
     2521 tgcacaattg aaattttaat tatgtatgaa ctgatagata tgaaatgaat attgaaaatg
     2581 agagcaataa aattttgaat gatgatgttg tgtgattgta taaacttatt cgtattttag
     2641 atacgttttg atatttcgca tgatgaggaa atacttttaa caactgatga catattttaa
     2701 tagtgaatat attttgagat attaatatat ttaatgttaa ggatgttgtc gcaataaaat
     2761 taaaatattg aaagataatt tttattgatt aattgcgaat atacaaagac accattatat
     2821 ccatatgcta aaataagaaa aaaggcgaca tgttatctca atttacctgc atctagactc
     2881 ctttacataa gaaataatca tcgccgagtt ttaaaatttt acaactttgt agatgtaaaa
     2941 agcatttgat tttaaatagt ttgaaaatca tatatttttg aaaaaggagc atgccgatga
     3001 gtattgacat gtatttagac agatctcgaa accaagcttc aagtgtgggg aatttgagtc
     3061 aaacaatgaa ttcaaattat gatgcgttgg aaaaagcaat tactcaattt attaatgatg
     3121 atgcgcttaa agggaaagcg tatacgtcag ctaagcaatt ttttagtacg gtgttaattc
     3181 cattatcaac aagtatgaaa acattgagtg atttaacgaa gcaagcttgc gataattttg
     3241 tgtcacgtta tacgagtgag gttgatagca tatctttaaa agaatcagag cttgaagaag
     3301 atatcagatc attaagtcaa caaattacgc gatatgaaaa tttgaataac aatttgaaaa
     3361 agcatgcttc cgataatcag caagccattt catcgaacca acaaataata cgaacattag
     3421 gtcaacaaaa acatgaatta gaagagaagc tacgcaaatt gcgtgagttt aatcaaaaat
     3481 caccagaaat atttaaagaa gttgaagaat ttcaaaaaat tgtccaacaa ggacttaccc
     3541 aagcgcagaa tttttggaac ttttcaacaa atcaatttaa tattccttca ggtaaagaac
     3601 ttgattgggc caaagcaagt catgaaaaat atttgaaagt tgctatgggg aaaattgaac
     3661 ataaagcaga gaaagaaact ttaaataaag cagactttgc tgttataaag gcatatgcca
     3721 aagaacatcc agaagacgat atcccgaaaa gtatattgaa atatataaat gacaataaag
     3781 acagtattaa aagagatata ggattagata ttacgtcaac acttttagag caaggcggta
     3841 taaatgcaag taaattcggt gtatttatca atacagcagg tggagtgaaa ggcccagcag
     3901 gtccaaattc atttgtggaa gtcaaacgta catcaggtaa tgtgtttata gaaaatggta
     3961 gtaaatttgc aaaaggcgga aaatacctag gtaaaggtgt tgctggtgta ggatttggta
     4021 taggtatgta tgatgacctt gcaaatgatg ataaaacatt tggagaggcg ttgtcgcata
     4081 atggtatgac gcttgcagct ggatctgcag ggacagcagt tggagctgga ttagcgactt
     4141 ttgttttagg aagtaatcca gtaggatggg tgattttagc aggtttggct atgagtacag
     4201 tatttgcatt aggaacagat ttaatttatc aaaataatat ttttggatta aaagataaag
     4261 tagactgggt agggcataaa atcgataata gtatagatgt tgttaaaaaa actacagaaa
     4321 aatccatgga t
//
LOCUS       DAKTIP010000196         4146 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAKTIP010000196 DAKTIP010000000
VERSION     DAKTIP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.199"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            195..3017
                     /gene="fnbB"
                     /locus_tag="O0305_002386"
     CDS             195..3017
                     /gene="fnbB"
                     /locus_tag="O0305_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCY0275497.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEAAASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETK
                     ATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTL
                     SDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLN
                     LFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGK
                     FSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDV
                     SKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYY
                     NYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGT
                     IEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYE
                     EDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELP
                     EEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQ
                     NSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQ
                     GGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHN
                     EGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGK
                     PIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTN
                     NGMLFGGLFSILGLALLRRNKKNHKA"
     gene            3197..4063
                     /gene="galU"
                     /locus_tag="O0305_002387"
     CDS             3197..4063
                     /gene="galU"
                     /locus_tag="O0305_002387"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCY0275498.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKALGL"
BASE COUNT         1631 a          633 c          828 g         1054 t
ORIGIN      
        1 gtgttcaaga gctttgtatg caatatatat gtgagtttca aataataaaa aactcattat
       61 acataaagtt aacaacaatc ttaacttttt attaactcgc tttttttcat tgcttttaaa
      121 aaccgaacaa tatagaattg catttattga gttttaaaaa taaatgaatt ttgcatttaa
      181 gggagaatat tatagtgaaa agcaatctta gatacggcat aagaaaacac aaattgggag
      241 cggcctcagt attcttagga acaatgatcg ttgttggaat gggacaagaa aaagaagctg
      301 cagcatcgga acaaaacaat actacagtag aggaaagtgg gagttcagct actgaaagta
      361 aagcaagcga aacacaaaca actacaaata acgttaatac aatagatgaa acacaatcat
      421 acagcgcgac atcaactgag caaccatcac aatcaacaca agtaacaaca gaagaagcac
      481 cgaaaactgt gcaagcacca aaagtagaaa cttcgcgagt tgatttgcca tcggaaaaag
      541 ttgctgataa ggaaactaca ggaactcaag ttgacatagc tcaaccaagt aacgtctcag
      601 aaattaaacc aagaatgaaa agatcaactg acgttacagc agttgcagag aaagaagtag
      661 tggaagaaac taaagcgaca ggtacagatg taacaaataa agtggaagta gaagaaggta
      721 gtgaaattgt aggacataaa caagatacga atgttgtaaa tcctcataac gcagaaagag
      781 taaccttgaa atataaatgg aaatttggag aaggaattaa ggcgggagat tattttgatt
      841 tcacattaag cgataatgtt gaaactcatg gtatctcaac actgcgtaaa gttccggaga
      901 taaaaagtac agatggtcaa gttatggcga caggagaaat aattggagaa agaaaagtta
      961 gatatacgtt taaagaatat gtacaagaaa agaaagattt aactgctgaa ttatctttaa
     1021 atctatttat tgatcctaca acagtgacgc aaaaaggtaa ccaaaatgtt gaagttaaat
     1081 tgggtgagac tacggttagc aaaatattta atattcaata tttaggtgga gttagagata
     1141 attggggagt aacagctaat ggtcgaattg atactttaaa taaagtagat gggaaattta
     1201 gtcattttgc gtacatgaaa cctaacaacc agtcgttaag ctctgtgaca gtaactggtc
     1261 aagtaactaa aggaaataaa ccaggggtta ataatccaac agttaaggta tataaacaca
     1321 ttggttcaga cgatttagct gaaagcgtat atgcaaagct tgatgatgtc agcaaatttg
     1381 aagatgtgac tgataatatg agtttagatt ttgatactaa tggtggttat tctttaaact
     1441 ttaataattt agaccaaagt aaaaattatg taataaaata tgaagggtat tatgattcaa
     1501 atgctagcaa cttagaattt caaacacacc tttttggata ttataactat tattatacaa
     1561 gtaatttaac ttggaaaaat ggcgttgcat tttactctaa taacgctcaa ggcgacggca
     1621 aagataaact aaaggaacct attatagaac atagtactcc tatcgaactt gaatttaaat
     1681 cagagccgcc agtggagaag catgaattga ctggtacaat cgaagaaagt aatgattcta
     1741 agccaattga ttttgaatat catacagctg ttgaaggtgc agaaggtcat gcagaaggta
     1801 ccattgaaac tgaagaagat tctattcatg tagactttga agaatcgaca catgaaaatt
     1861 caaaacatca tgctgatgtt gttgaatatg aagaagatac aaacccaggt ggtggtcagg
     1921 ttactactga gtctaaccta gttgaatttg acgaagattc tacaaaaggt attgtaactg
     1981 gtgctgttag cgatcataca acaattgaag atacgaaaga atatacgact gaaagtaatc
     2041 tgattgaact agtagatgaa ctacctgaag aacatggtca agcgcaagga ccaatcgagg
     2101 aaattactga aaacaatcat catatttctc attctggttt aggaactgaa aatggtcacg
     2161 gtaattatgg cgtgattgaa gaaatcgaag aaaatagcca cgtggatatt aagagtgaat
     2221 taggttacga aggtggccaa aatagcggta atcagtcatt tgaggaagac acagaagaag
     2281 ataaaccgaa atatgaacaa ggtggcaata tcgtagatat cgatttcgat agtgtacctc
     2341 aaattcatgg tcaaaataat ggtaaccaat cattcgaaga agatacagag aaagacaaac
     2401 ctaagtatga acaaggtggt aatatcattg atatcgactt cgacagtgtg ccacatattc
     2461 acggattcaa taagcacact gaaattattg aagaagatac aaataaagat aaaccaaatt
     2521 atcaattcgg tggacacaat agtgttgact ttgaagaaga tacacttcca caagtaagtg
     2581 gtcataatga aggtcaacaa acgattgaag aagatacaac acctccaatc gtgccaccaa
     2641 cgccaccgac accagaagta ccaagcgagc cggaaacacc aacaccaccg acaccagaag
     2701 taccaagcga gccggaaaca ccaacaccgc caacgccaga ggtaccaact gaacctggta
     2761 aaccaatacc acctgctaaa gaagaaccta aaaaaccttc taaaccagtg gaacaaggta
     2821 aagtagtaac acctgttatt gaaatcaatg aaaaggttaa agcagtggta ccaactaaaa
     2881 aagcacaatc taagaaatct gaactacctg aaacaggtgg agaagaatca acaaacaacg
     2941 gcatgttgtt cggcggatta tttagcattt taggtttagc gttattacgc agaaataaaa
     3001 agaatcacaa agcataatca atccaaaatt gacaggttta tttcataaat tatatgaagt
     3061 aagcctgttt tttaaaatta aaacaaattt cccaagaaat aattacatac tcaatgacac
     3121 tatgaaggcg ttctaattag tgttaaaatg acgttgatac atagatttaa tacttaggaa
     3181 aaggagcaca ttaactttga aaaaaataaa aaaggcaatc attcccgctg ctggtttagg
     3241 gactagattt ttaccagcaa ctaaagcgat gccaaaggaa atgcttccta tcttagataa
     3301 acccacaata caatatatcg ttgaagaagc tgcaagagct ggaattgaag atattattat
     3361 agtgacaggt cgccacaaac gcgcgattga agatcatttt gatagtcaaa aagaattaga
     3421 aatggtgtta aaagaaaaag gtaaatctga attactagag aaagttcagt attcaacgga
     3481 acttgcgaat attttttatg taaggcagaa agaacaaaaa ggtttagggc atgcgattag
     3541 ttctgcgcgt caatttatcg gcaatgaacc atttgcggta ttattaggtg acgatattgt
     3601 tgaatctgaa gtgccagcag tgaaacagtt gattgacgtt tatgaagaaa cagggcactc
     3661 agttataggt gtccaagaag ttccggaagc agatactcat cgttatggaa ttattgaccc
     3721 attaactaaa aacggacgtc agtatgaagt gaaaaagttt gttgaaaagc cagcacaagg
     3781 tacggcacca tcaaacctag ctattatggg acgttatgta ttaacgcctg aaattttcga
     3841 ttatttaaaa acgcaaaaag aaggcgcagg taacgaaatt cagttaacag atgcgattga
     3901 acgtatgaat aatgataacc aagtgtatgc atatgacttc gaaggcgaac gttatgacgt
     3961 tggagaaaaa ttaggcttcg ttaaaactac catagaatat gcattgaaag atgacagtat
     4021 gcgagaagaa ttaacacgat ttattaaagc attaggttta taacattaaa agcatgatac
     4081 tcagtaaaat tggaacacga aatggtgaac taaattgaat gagtgtcatg cttaatttat
     4141 gtgttg
//
LOCUS       DAKTIP010000197         4119 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAKTIP010000197 DAKTIP010000000
VERSION     DAKTIP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.200"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            93..1211
                     /gene="ald"
                     /locus_tag="O0305_002388"
     CDS             93..1211
                     /gene="ald"
                     /locus_tag="O0305_002388"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCY0275499.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1306..2346
                     /gene="tdcB"
                     /locus_tag="O0305_002389"
     CDS             1306..2346
                     /gene="tdcB"
                     /locus_tag="O0305_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCY0275500.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2377..3699
                     /locus_tag="O0305_002390"
     CDS             2377..3699
                     /locus_tag="O0305_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0275501.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3856..>4119
                     /locus_tag="O0305_002391"
     CDS             3856..>4119
                     /locus_tag="O0305_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0275502.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIIL"
BASE COUNT         1474 a          582 c          809 g         1254 t
ORIGIN      
        1 atatgaataa ttaattaatg gtatttacat attcataacc aaaggataaa ataattgtga
       61 aattattcac aaatgaaaga ggagtgttgc aaatgttagt tgcagtagtc aaagaattaa
      121 aacaaggtga aggacgtgta gcttgcacac ccgaaaatgt gcgtaagtta acggatgcag
      181 gacataaagt aattgttgaa aaaaatgctg gcattggttc aggattttct aacgatatgt
      241 atgaaaaaga aggcgctaag atcgtaactc acgaacaagc atgggaagct gatcttgtta
      301 tcaaagtaaa agaacctcat gaaagcgaat atcaatattt caaaaagaat caaattatct
      361 ggggattttt acatctagca tcttcaaaag aaatagtaga aaaaatgcaa gaagttggtg
      421 taactgcgat tagtggtgaa accattataa aaaatggaaa agcagaatta ttagcgccaa
      481 tgagtgctat agcaggtcaa cgctcagcaa ttatgggagc ttactactct gaagcacaac
      541 atggtggtca aggtacttta gtgactggtg tacatgaaaa tgtggatata cctggtagta
      601 catatgtgat tttcggtggt ggagtagcag caacaaatgc agcaaatgtt gccttgggac
      661 taaatgctaa agtaatcatt atcgagttaa acgatgaccg cattaaatat cttgaagata
      721 tgtatgcaga aaaagatgtc acagtagtca aatcaacacc agaaaattta gcagaacaaa
      781 ttaagaaagc agatgtattt atttctacaa ttttaattcc aggtgcgaaa ccgccaaaat
      841 tggttactcg tgagatggtt aaatcaatga aaaaaggttc agtattaatc gatatagcta
      901 ttgaccaagg tggaactatt gaaacaatta gaccaactac aatttctgat ccagtgtatg
      961 aagaagaagg tgtgattcat tatggtgtac caaatcaacc aggagcagtc ccaagaactt
     1021 caacaatggc attagcacaa ggaaatattg attatatatt agaaatttgt gacaaaggct
     1081 tagaacaagc aattaaagat aatgaagcct taagtactgg tgtaaacatt taccaaggac
     1141 aagtgacaaa tcaaggatta gcttcatcac atgacctaga ttataaagaa atattaaatg
     1201 ttatcgaata gatagtaatt taaatgaaat tgagtgaaat gaatatttta aatatagcat
     1261 tatagtttgg actaaaaatt tacaaaacgg aaggatgtaa ttattatgac aaccaacaca
     1321 gttacattac aaacagcaca tattgtaagt ttaggagata tcgaagaagc taaagcaagc
     1381 attaaaccat ttattcgtcg aacacctcta attaaatcaa tgtatttaag ccaaagtata
     1441 actaaaggga atgtatttct aaaattagaa aatatgcaat tcacaggatc ttttaaattt
     1501 agaggcgcta gcaataaaat taatcactta acagatgaac aaaaagaaaa aggcattatc
     1561 gcagcatctg ctgggaacca tgcacaaggt gttgctttaa cagctaaatt attaggcatt
     1621 gatgcaacga ttgtaatgcc tgaaacagca ccacaagcga aacaacaagc aacaaaaggc
     1681 tatggggcaa aggttatttt aaaaggtaaa aactttaacg aaactagact ttatatggaa
     1741 gaattagcga aagaaaatgg catgacaatc gttcatccat atgacgataa gtttgtaatg
     1801 gcaggccaag gaacaattgg tttagaaatt ttagatgata tttggaatgt gaatacagtc
     1861 atcgtaccag ttggcggtgg aggattaatt gcaggtattg ccaccgcatt aaaatcattt
     1921 aacccttcaa ttcatattat cggtgttcaa tctgagaatg ttcatggtat ggctgagtct
     1981 ttctataaga gagatttaac tgaacatcga gtggatagca caatagcaga tggttgtgat
     2041 gtaaaagttc ctggtgaaca aacatatgaa gtagttaaac atttagtaga tgaatttatt
     2101 cttgttactg aagaagaaat tgaacatgct atgaaagatt taatgcagcg tgccaaaatt
     2161 attactgaag gtgcaggcgc attaccaaca gctgcaattt taagtggaaa aataaacaat
     2221 aaatggcttg aagataaaaa tgttgttgca ttagtttcag gcgggaatgt tgacttaact
     2281 agagtttcag gtgtcattga acatggactg aatattgcag atacaagcaa gggtgtggta
     2341 ggttaaaaca tttaatctta aaaatgaggt gtaattatgt caaatggtaa agaattacaa
     2401 aaaaatatag gtttcttctc agcgtttgct attgttatgg ggacagttat tggttcagga
     2461 gtattcttta aaatatcaaa cgtaacagaa gtaacaggaa cagcaggaat ggccttgttt
     2521 gtatggttcc taggcggcat cattaccatt tgtgcggggt taacagcagc agaacttgct
     2581 gctgcaatcc ctgaaacagg tggcttaacg aagtatatag aatatacata cggtgatttc
     2641 tggggcttcc tatcaggttg ggcgcaatca tttatttatt ttccagctaa cgtagcagca
     2701 ttgtctatcg tatttgcgac acagctaatt aatttattcc atttatctat aggttcgtta
     2761 ataccaatag caatcgcatc tgcgttatct attgtgttga taaatttcct aggttcaaaa
     2821 gcaggcggaa ttttacaatc agttacttta gtaattaaac tgattccaat catcgttatt
     2881 gtaatttttg gtatttttca atctggagat atcacttttt cattaattcc aactacaggt
     2941 aattcaggaa atggcttctt tacagcaatt ggtagtggtt tattagcaac tatgtttgca
     3001 tatgatggtt ggattcatgt aggaaatgtt gcgggggaac ttaaaaatcc taaacgcgat
     3061 ttacctttag cgatttcagt tggtatcggt tgtattatgg ctgtgtattt attaattaac
     3121 gctacattct tattaacgtt gccaatagaa ctacttgctg gtaatttaaa tgcagcttca
     3181 gatacatcaa aaatattatt tggtgaaaat ggcggtaaga ttattacaat cggtatatta
     3241 atttctgttt atggtacgat caatggctat actatgactg gtatgcgcgt accatatgca
     3301 atggctgaaa gaaaattatt gccatttagc catttattcg caaaattaac aaaatctggc
     3361 gcaccatggt ttggcgcaat tatacaactt ataatcgcta tcatcatgat gtcaatggga
     3421 gcatttgata caattacaaa tatgttaatc tttgttattt ggttgttcta ttgtatgtca
     3481 tttgttgcgg taataatttt aagaaaacgt gaaccaaata tggaacgacc atataaagta
     3541 ccgttatatc cgatcatacc tttaattgct attttggcag gatcatttgt attaattaat
     3601 acactgttta cacaatttat attagcaatc attggaattc taataacagc acttggtata
     3661 ccagtttatt actataaaaa gaaacaaaaa gcagcataag gtaagataac tagcattgag
     3721 aataaatgga tggactacta ataaatttaa agttttacac attaaaatca aaaaccattc
     3781 aattattcta tggaacagac aaatttctgt tatggaattt gtctgttttt caaaagtata
     3841 gggaggcaaa tagagatgga aaagccgtca agagaggcat ttgaaggcaa taataagttg
     3901 ttaataggaa ttgttctaag tgtaataacg ttttggctat ttgcacaatc attggttaat
     3961 gttgtaccaa tacttgaaga tagtttcaat acagatattg gaacggttaa tatcgccgtt
     4021 agtataactg ctttattttc aggaatgttt gtagtaggag caggtggtct tgctgataaa
     4081 tatggcagaa ttaaactcac gaacattggt attatctta
//
LOCUS       DAKTIP010000198         4097 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAKTIP010000198 DAKTIP010000000
VERSION     DAKTIP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.201"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            749..2812
                     /locus_tag="O0305_002392"
     CDS             749..2812
                     /locus_tag="O0305_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCY0275503.1"
                     /translation="MNNNGEEHNHQNHMNHSNQMHHDNHASHDHHSGHAHHHGNFKVK
                     FFVSLIFAIPIILLSPLMGVNLPFQFTFPGSEWVVLILSTILFFYGGKPFLSGGKDEI
                     ATKKPGMMTLVALGISVAYIYSLYAFYMNNFSSATGHTMDFFWELATLILIMLLGHWI
                     EMNAVGNAGDALKKMAELLPNSAIKVMDNGQREEVKISDIMTDDIVEVKAGESIPTDG
                     IIVQGQTSIDESLVTGESKKVQKNQNDNVIGGSINGSGTIQVKVTAVGEDGYLSQVMG
                     LVNQAQNDKSSAELLSDKVAGYLFYFAVSVGVISFIVWMLIQNDVDFALERLVTVLVI
                     ACPHALGLAIPLVTARSTSIGAHNGLIIKNRESVEIAQHIDYVMMDKTGTLTEGNFSV
                     NHYESFKNDLSNDTILSLFASLESQSNHPLAISIVDFAKSKNVSFTNPQDVNNIPGVG
                     LEGLIDNKTYKITNVSYLDKHKLNYDDDLFTKLAQQGNSISYLIEDQQVIGMIAQGDQ
                     IKESSKQMVADLLSRNITPVMLTGDNNEVAHAVAKELGISDVHAQLMPEDKESIIKDY
                     QSDGNKVMMVGDGINDAPSLIRADIGIAIGAGTDVAVDSGDIILVKSNPSDIIHFLTL
                     SNNTMRKMVQNLWWGAGYNIVAVPLAAGILAFIGLILSPAIGAILMSLSTIIVAINAF
                     TLKLK"
     gene            2830..3375
                     /locus_tag="O0305_002393"
     CDS             2830..3375
                     /locus_tag="O0305_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1541 domain-containing protein"
                     /protein_id="HCY0275504.1"
                     /translation="MIKKLFFMILGSLLILSACSNNDEKDKDTNDQKSESHMKHNDES
                     KVPEDMKSTNEGEFKVGDKVTITAGHMPGMKGAEATVKGAYKTYAYVVSYKPTNGNEK
                     VNNHKWVVNEEIKDAPKDGFSKGDTVKLEASHMSGMKGATANIDNVKKTTVYVVDYKS
                     KDNGKIIKNHKWMTGNELKAR"
     gene            complement(3803..3937)
                     /locus_tag="O0305_002394"
     CDS             complement(3803..3937)
                     /locus_tag="O0305_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275505.1"
                     /translation="MKLHWYIYYFLSISVILFMIIYHYLATSMTVDYEITVSIAKLLL
                     "
     gene            3901..3993
                     /locus_tag="O0305_002395"
     CDS             3901..3993
                     /locus_tag="O0305_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275506.1"
                     /translation="MTKNSKYTNEVSKEQFQEIENVNNKVKEFY"
BASE COUNT         1521 a          542 c          723 g         1311 t
ORIGIN      
        1 ctgagcttga acttatctct aaatcatttt gagctactta tctatcaatt catatattct
       61 ataacaatat ttgtgacatc acgtgctatt tcatgaagtg attttacgat atcaccttct
      121 ttagaaaaaa tatatttcag taggcaccga cgtatacaga atcatttgag tattaaaaat
      181 aaaaactaga aaaagaaacc gcatcattaa ctgatacgca gaatcatatt ataaataaaa
      241 ctaaaaatga ggttgtatat aactcactct gaaattgatt gaatatatag tatctttaat
      301 aaaatgcagc tattgtggcg tagaatttga gaatcaaaaa atgattaata tagtttgaag
      361 agactgagca taaatactag aaaaatggcc agtaaatgag tttactataa actcatttac
      421 tggcttctct aataattatc aagacaattt gcgtttctag gcatactttg aaatgcgcta
      481 ttttctttaa gaatattaat atatgacttg tgttggtaat gataaccttg gttagagtgt
      541 attgtcatgt gatagttaag attagatctt ttaattaaaa cattgtttta aatatcgatg
      601 acaaggtcta atgtaggacg tgtagatata aggttcggaa ttatataaat ccataaaagg
      661 agataaatcg aaaaccttta taccctgggt gggtatatgt tatagtataa gtagctttac
      721 tataacattt tcattaggag gggttaattt gaataataat ggtgaagagc ataatcatca
      781 aaatcacatg aatcattcca atcaaatgca tcatgataac catgcctcac atgatcatca
      841 tagtggccat gcacatcatc atggaaattt taaagttaag ttttttgttt cattaatttt
      901 tgcaatacct atcattcttt tatcgccact gatgggtgtt aacttacctt ttcaattcac
      961 atttccaggt tctgaatggg tagtgttaat attaagtaca attttattct tttatggtgg
     1021 taaaccgttc ttgtctggtg gtaaagatga aattgctaca aaaaaaccag gcatgatgac
     1081 cttagttgcc ctaggtattt cagtagctta tatttatagc ttgtatgctt tttatatgaa
     1141 taactttagt agtgcaactg gtcatacaat ggactttttt tgggaattag caaccttaat
     1201 tttaattatg ctattaggac attggataga aatgaatgct gtcggaaatg ctggagatgc
     1261 tttaaagaaa atggcagaac tgttacctaa tagtgctatt aaagttatgg ataatggcca
     1321 acgcgaagaa gttaaaatat cagacatcat gactgatgat atcgtcgaag taaaagccgg
     1381 agaaagcatt ccaacagatg gtattatcgt tcaaggacaa acatctatag atgaatccct
     1441 agtcactgga gaatctaaaa aagtacaaaa aaatcaaaat gacaacgtca tcgggggttc
     1501 tattaatggg tctggaacaa tacaagtcaa ggttacagct gtgggagaag atggatatct
     1561 ttctcaagtt atgggacttg ttaatcaagc acaaaatgat aaatctagtg ctgaattgtt
     1621 atctgataaa gtagcgggtt atttattcta ctttgctgta agtgttggcg tgatttcttt
     1681 tattgtctgg atgctcattc aaaatgatgt tgattttgca ttagaacgtc ttgtaactgt
     1741 gttagtcatt gcttgtccac atgctttagg cttggcaata cctttagtca ctgcacgttc
     1801 tacttcaatt ggtgcacata atggtttaat tattaaaaat agagagtctg tagaaatagc
     1861 tcaacatatc gattatgtaa tgatggacaa aactggtact ttaactgagg gtaacttttc
     1921 tgtgaatcat tatgagagct ttaaaaatga tttgagtaat gatacaatat taagcctttt
     1981 cgcctcatta gaaagtcaat ctaatcaccc attagctata agtattgttg attttgcgaa
     2041 aagtaaaaat gtttcattta ctaacccaca agacgttaat aatattccag gtgtcggatt
     2101 agaaggtcta attgataata aaacatataa aataacaaat gtctcttatc ttgataaaca
     2161 taaacttaat tatgacgatg acttgtttac taaattagct caacaaggta attcaatcag
     2221 ttatttaatt gaggatcaac aagtcattgg catgattgct caaggagatc aaattaaaga
     2281 aagctcaaaa caaatggtag ctgatttact atcaagaaat attacaccag tcatgcttac
     2341 aggtgacaat aatgaagtgg cacacgctgt cgcaaaagaa ttaggtatta gtgatgtcca
     2401 cgcacaactc atgccagaag ataaggaaag cattataaaa gattatcaaa gtgacggtaa
     2461 taaagtcatg atggtcggag acggtatcaa cgatgcgccg agtcttataa gagcggatat
     2521 tggtatagca attggtgcag gtacagatgt tgcagtggat tcaggtgata tcatacttgt
     2581 taaaagtaat ccatcagata tcattcattt cttgaccctt tcaaataata ctatgagaaa
     2641 aatggtgcaa aacttatggt ggggtgcagg ttataatatt gttgctgtac ctttagcagc
     2701 tggtatttta gcatttattg gcttgatttt atcacctgca ataggtgcta ttttaatgtc
     2761 tttaagtaca attatcgttg caattaatgc ctttacatta aaattaaaat aaaagatagg
     2821 agttttatta tgattaaaaa attatttttt atgatattag gatcattact aatattatca
     2881 gcttgctcca ataatgatga aaaagataaa gacactaatg accaaaaaag tgagagccat
     2941 atgaagcata atgatgaaag taaagttcca gaagatatga aatcgactaa tgagggtgaa
     3001 tttaaagtgg gagataaagt aacgattaca gcagggcata tgccaggtat gaaaggtgca
     3061 gaagctactg taaaaggtgc gtataaaaca tatgcctatg ttgtaagtta taaacccaca
     3121 aatggaaatg aaaaagtaaa caatcataaa tgggtcgtaa acgaagagat taaagatgca
     3181 cctaaagatg gatttagtaa gggcgatact gttaaattag aagcaagtca tatgtctggt
     3241 atgaaaggtg ctacagccaa tatagataac gtgaaaaaga ctactgttta cgtagttgat
     3301 tacaaatcca aagataatgg taaaatcatt aaaaatcata aatggatgac aggaaatgag
     3361 ctgaaagcac gataaaaatc tagttctaaa ttgagaaata aatagatata aaaatatcct
     3421 ccttaatcaa taatttaaat aacttattat tgttaaggag gatatttttt agtgtgtaaa
     3481 ttaaaaagaa ttttagaaga ataacattta tcaaaaaact gttcattacc ttattaattg
     3541 aaattatata attaaaaacc gcatcattaa ccgatacgca gaggcgtatc ataagtaaaa
     3601 ctaaaaaatt ctgtatgagg agataataat ttggagggtg ttaaatggtg gacattaaat
     3661 ccacgttcat tcaatatata agatatatca cgataattgc gcatataact taagtagtag
     3721 ctaacagttg aaattaggcc ctatcaaatt ggtttatatc taaaatgatt aatatagaat
     3781 gcttcttttt gtccttatta aattataaaa gtaactttgc aatagaaaca gttatttcat
     3841 aatcaacagt cattgacgta gctaagtaat gataaataat cataaataaa attacagata
     3901 ttgacaaaaa atagtaaata taccaatgaa gtttcaaaag aacaattcca agaaattgag
     3961 aatgtaaata ataaggtcaa agaattttat taagatttga aagagtatca atcaagaaag
     4021 atgtagtttt ttaataaact atttggaaaa taattatcat aatttaaaaa ctgacaattt
     4081 gcgagactca taaaatg
//
LOCUS       DAKTIP010000199         4093 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAKTIP010000199 DAKTIP010000000
VERSION     DAKTIP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.202"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            15..565
                     /locus_tag="O0305_002396"
                     /pseudo
     CDS             15..565
                     /locus_tag="O0305_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
     gene            558..1778
                     /locus_tag="O0305_002397"
     CDS             558..1778
                     /locus_tag="O0305_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0275507.1"
                     /translation="MTDLLKIKNFRLFFLAEIISAFGVGISTVGANWYLIDKTNDSQL
                     LGIMLALNVLSGFLASPIIGGLADKYNRRNIILITYLLQVILYLLIVIALVMIGFETY
                     LVIGFAIVNGIGWTTYMATSRSLVKQILKPDQYTDANSLLEISLQTGMFIAGGLSGIL
                     YKINGFTLIIAMTIMMFLISIFMLFRLHVDKPTHSEEESTNSLLQEYLLGWKFLKDNM
                     MIFIFGVISIIPMVFTMIFNISLPGYVYNVLKLSSVQFGFSDMLYGIGGLCAGLISAI
                     LSKKISTKVLIFLLYFILVINSALFIWINSAFYLFIGSFILGYSISSIRIYMNTAIMN
                     TVSDKYVGRSFTIWTSISLLLQSLIAPFLGRWINEINDKFGFYIILILSLLIFVTLLL
                     VNKTGKIKYAHKEE"
     gene            complement(1850..2146)
                     /locus_tag="O0305_002398"
     CDS             complement(1850..2146)
                     /locus_tag="O0305_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCY0275508.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKRRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGSKEMILILDFTEATNLTDLFKTIKNDLKK"
     gene            complement(2195..2398)
                     /locus_tag="O0305_002399"
     CDS             complement(2195..2398)
                     /locus_tag="O0305_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275509.1"
                     /translation="MEGLKHSLKSLGWWDLFFAIPIFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            2707..3693
                     /locus_tag="O0305_002400"
     CDS             2707..3693
                     /locus_tag="O0305_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCY0275510.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
BASE COUNT         1415 a          636 c          673 g         1369 t
ORIGIN      
        1 aaggagttat gactatgatt acttgtttat ttggaagtag tagaaaggtg gcaattcagc
       61 tatcgcagta gagaatctat tgaagaattt aaaagtcaac tttgtgaatt tatatcaaag
      121 caacattgaa aaggttacag ataatcgtca tagcaaaggt gccaactttc ataatgatga
      181 ttacgaacaa attttaatca aagttttaga atcagatatt attatttttt caacaccact
      241 ctattggtac tccatgagtg cgtctttaaa actcttcatt gatagatgga ccgagtcatt
      301 gcgagatacg caaatagaca acttcaaaga aatcatgtca caaaaaaagt atttgatttt
      361 aatcataggt ggcgacaatc cgcataccaa agcacaacct ttagtcaacc aattcaaatt
      421 aatatttgag tttatgaata ttactaacta tagatttttg atcggcgaag gcaataaacc
      481 attcgatatt ttaaacgact ctcaatttat agaagagctc gcaaatataa atcttgcatt
      541 gaaaaaaggt gatatttatg actgatttgt tgaaaattaa aaacttcaga ttgttctttt
      601 tagcagaaat aatttcagca ttcggagttg gaattagtac agttggggct aattggtatt
      661 taatcgacaa aactaacgac tcccaactat taggtattat gttagcttta aatgtattat
      721 ccggtttctt agcttctcct ataataggtg gactagccga taaatataat agacgaaata
      781 ttattttaat cacctatctt cttcaggtaa tattatattt actcattgtc atagcactcg
      841 taatgatagg atttgagacc tatttagtga ttggatttgc gattgttaat gggataggtt
      901 ggactacgta tatggcaaca tctagaagtt tagttaaaca gatattaaaa ccagatcaat
      961 atacagatgc aaattctcta ttagagatta gtttgcaaac aggtatgttt attgcaggtg
     1021 gattatcagg aatattatat aaaataaatg gattcactct aattatagcg atgactataa
     1081 tgatgtttct aatcagcatt tttatgttat ttagattgca tgtagataaa ccaactcatt
     1141 cagaggaaga atcaacaaat agcttattgc aagaatattt attaggatgg aaatttttaa
     1201 aagacaacat gatgatattt atttttggag ttatttcaat cataccaatg gtgtttacaa
     1261 tgatctttaa catatcatta ccaggatatg tttataatgt tttaaaactt tcgtctgtac
     1321 aatttggttt ttcagatatg ttatatggca ttggaggatt atgtgcaggt ttaatttcag
     1381 ctattctttc gaagaaaatc tcaactaaag tattgatatt tttgttatat ttcatattag
     1441 tcataaattc agcactattt atttggataa actcagcatt ttacttattc ataggatcat
     1501 ttatactagg ctactcaatt tcatcaatca gaatttatat gaatacagct ataatgaaca
     1561 ctgtttcaga taaatatgtc ggtcgctcat ttacgatatg gacgtcaatt tcattgttac
     1621 tgcaaagttt aattgctcca tttttaggaa gatggattaa tgaaattaat gataaattcg
     1681 gtttctatat tatactcatt ttatccttac tcatatttgt tacactgctg cttgttaaca
     1741 aaacaggcaa aataaaatat gcacataaag aagagtgacc gtcactcttc tttaacaagc
     1801 gaccatttat cgatgggctt agttctctct gcacccacac ttcactactt cactttttca
     1861 aatcattttt tatggtctta aataaatcag tgagatttgt tgcttcggta aagtctagaa
     1921 ttaatatcat ttctttagaa cctggatatg gcgaaactaa tgtattatct tgcaatttct
     1981 gctgggcact tttagtcgcc ttgaccaata atctattatc atacaaacca cctataacca
     2041 cgccatcata ataaataata tattctccca tcatctttct tctcttaacc gcgtttgaat
     2101 tcacatgatt taaaaataaa tcatgtacat ctttcttagt cgccattgta atcgctcctt
     2161 cagttttatg tttaatcaca ttcgtattaa tgattcattt cgtgttgctc ttaattttat
     2221 ctataattat atgcgtagtt aaaatcaaac ctatggaaaa gaaaataatg ataacaatgt
     2281 taagaaatat agttataaaa ttatagtttg gaaggtatgc gaatagcaga aaaataggta
     2341 tcgcaaaaaa taaatcccac caacctaaac tttttaaaga atgctttaaa ccttccataa
     2401 tatcaccttt ataaatttgt ctttgttata agataactaa aaaatcgctt tactgtaaaa
     2461 gtagccaaag aaaattctga atcatattca taagtagtgt atcattaata atgaacaatt
     2521 taatactata atccttgatc tttgtattga tcaacttacc acaacattta ttttagacta
     2581 ctcttagact tccctttcaa atggttgcat ctattgaaat tccttttgta taagttaggc
     2641 ttttgtggta atatcatcat gcataaaaaa tcgagatact aattataaag agggtataaa
     2701 tatattatga aagaaaattt ttggagtgaa ttaccacgtc cattttttat tttggcgcca
     2761 atggaagacg ttacagatat cgtctttcga cacgttgtaa gtgaagcagc tagaccggat
     2821 gtgtttttca ctgaatttac aaatactgaa agcttttgcc accctgaagg catacatagt
     2881 gtgcgcggac gcttaacttt tagtgaagat gaacagccga tggtcgctca tatatgggga
     2941 gataagccag aacagttccg tgaaacgagt attcaattag ctaaaatggg ctttaaaggc
     3001 atagacttaa atatgggatg tcctgtagca aatgttgcta aaaagggtaa gggttccggc
     3061 ttaatcttaa gacctgacgt tgctgccgaa attattcaag cgactaaagc aggtgggctt
     3121 ccggtaagtg ttaaaacacg ccttggctac tatgaaatcg atgaatggaa agattggttg
     3181 aagcacgtct tcgaacaaga cattgccaat ttatctattc atcttcgtac acgtaaagaa
     3241 atgagtaaag tagatgcaca ttgggaatta atcgaagcta ttaaaaattt acgtgacgaa
     3301 attgcaccaa atacattgtt aacaattaac ggtgatattc ccgatagaaa aacaggactt
     3361 gaactggcag aaaaatatgg cattgatggc gtcatgattg gtagaggcat tttccacaat
     3421 ccattcgctt ttgaaaaaga accacgcgaa cacacaagca aggaactatt agatctattg
     3481 agattgcatt tatcattgtt taacaaatat gaaaaagatg aaatacgaca attcaagagc
     3541 ttgcgtagat tctttaaaat ctatgtgcgt ggcataagag gcgctagcga acttcgacat
     3601 caattgatga acacacaatc aattgcagaa gcacgagcac tactcgatga atttgaagcc
     3661 caaatggacg aagacgttaa aattgaatta tagtatgagt aataaagttt atggatgata
     3721 tttcccaatt taacacggat tgaacacttt ctacttaggt attatcttgg ttttcctgat
     3781 aggataactc ccgatgatgc ctttttactt atgtcactta tccttttgga aatggtgcaa
     3841 aattaaaaat acatcaggat atcgtttaac tcagaaaaaa actccaatca aaaaaaatgt
     3901 ttacaacaca ttatgaaatt aaaattgctc ttagtgttca acagacaaag ttattgaaat
     3961 tactattgga ggtacatacc aagctatcat tttgagtaca cgtggtttga atatctggca
     4021 agaaatggaa attggtaatg tcagtcataa agtcgctaaa cgtgctcaaa taccattaat
     4081 tatttttaaa taa
//
LOCUS       DAKTIP010000200         4063 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.203, whole genome shotgun sequence.
ACCESSION   DAKTIP010000200 DAKTIP010000000
VERSION     DAKTIP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.203"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            221..535
                     /locus_tag="O0305_002401"
     CDS             221..535
                     /locus_tag="O0305_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCY0275511.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            535..1827
                     /gene="arsB"
                     /locus_tag="O0305_002402"
     CDS             535..1827
                     /gene="arsB"
                     /locus_tag="O0305_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCY0275512.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLIGLYLTLIIF"
     gene            1845..2240
                     /gene="arsC"
                     /locus_tag="O0305_002403"
     CDS             1845..2240
                     /gene="arsC"
                     /locus_tag="O0305_002403"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCY0275513.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILADDWNVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPSLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKIAIENFKSR"
     gene            complement(2535..3389)
                     /locus_tag="O0305_002404"
     CDS             complement(2535..3389)
                     /locus_tag="O0305_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCY0275514.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(3665..3889)
                     /locus_tag="O0305_002405"
     CDS             complement(3665..3889)
                     /locus_tag="O0305_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275515.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT         1315 a          647 c          593 g         1508 t
ORIGIN      
        1 actaaggtga ttggccatac actcaagata tggctaatca cctttttcaa tgcagaacaa
       61 agagtatatt ttgcaaaatc aatatctata atttatcttt aatacccttt taatactaaa
      121 accaaattta aattacatag tcatatcgtg taatatacca aaggaacata gacattcatc
      181 tatatagatg ataatctatt taacgtctag gaggtatgtt atgacgtata aagaactagc
      241 aacattttta aaagttttat cagattcaag cagattagaa atactagatt tactttcttg
      301 tggagagtta tgcgcttgtg atttgttagc acattttcaa ttctctcaac ctacacttag
      361 ctatcatatg aaagcattag taaaaaccaa cttagttacg acacgaaaaa tcggaaataa
      421 acatttatac cagctcaatc ataatatttt tgagtccgta attaataact tgtctaaggt
      481 tcatacctct aaccaacgat gtatttgtca taaccttaag actggtgaat gctaatgatg
      541 acaactttag cgacactcat ttttctagta actttattat ttgtattatg gcaacctaaa
      601 ggcctagata ttggcattac cgcactaact ggtgccttta ttgctgttat tactggtgtt
      661 gtaagttttt ccgatgtttt cgaagtaaca ggtattgttt ggaatgctac tttgactttt
      721 gtctcagtca ttcttatttc attaatatta gataaagttg gattattcga atggtcagct
      781 attcacatgc ttcatgcttc aaaaggcaat ggtttaaaaa tgttcgttta tatcatatta
      841 ttgggtgcca ttgttgctgc atttttcgca aatgatggcg cagcgttaat cttaacgcct
      901 attgtattag cgatggttaa aaatataggt tttagtaagc gggccatatt cccttttatt
      961 attgcgagtg gttttatagc tgacacaact tctttacctt taatcgtgag caatctagtg
     1021 aatattatat ctgctgatta ttttcatgta ggattcgttc gatattttag tagaatgatt
     1081 atacctaatt tattctcgct tttagcaagt attatagtat tgtggttata ttttagaaaa
     1141 gcgataccta aaacgtttga tgataataat ataaagcatc ctaaagatgc cattaatgat
     1201 ttaaagctat ttaaaatttc atggattgtt ctagttatat tacttttcgg ctatctaatc
     1261 agtgaattta ctaaaattcc ggtatcaatt ttcactggaa tcattgcttt tatttttcta
     1321 atgttggctc gtaaatcaaa tgctgtaaat attaagcaag tcattaaggg cgcaccttgg
     1381 aatatagtat tattttcaat tggtatgtat atcgtcgtat tcggcttaag aaatgctggc
     1441 attactttaa tattggctaa aatattagaa tatatttcca attacggtct atttagcact
     1501 attttgggaa tgggcttcat ttcagcgttt ttatcatcaa taatgaataa tatgcctaca
     1561 gttttaatag atgcgattgc tattggtcaa tcaaatgtcc atggcatgtt aaaagaaggc
     1621 ctaatttatg cgaatgttat cggttctgat ttaggtccaa aaattacacc gataggctct
     1681 ttagctacat tactgtggtt acacgtctta acacaaaaag atgttaagat ttcttggggc
     1741 acatacttta aaactggtat catcattaca attccagtac tatttataac cctcataggg
     1801 ttgtatctaa cacttatcat attttaatta attaggagat tgttatgact aaaaaaacaa
     1861 tttattttat atgtacaggc aactcatgtc gaagtcaaat ggctgaaggt tgggctaaac
     1921 aaatcttagc ggatgattgg aatgtatatt ctgctggtat cgaaacacac ggtgttaatc
     1981 ccaaagcgat agaagctatg aaagaagtag gcattgatat atcaaatcat acatcagatt
     2041 taatcgataa taatattatt aaaaattcaa atttagttgt tacattatgt agtgatgcag
     2101 acgtaaattg cccttcttta ccaacaaatg ttaagaaaga acattgggga tttgatgatc
     2161 ctgcaggcaa gccttggtca gagttccaac gtgtaagaga tgaaattaaa atcgcaattg
     2221 aaaatttcaa atcacgatga gagtgggaca gaaatgataa aaaaacacta atgatttatt
     2281 atgtagtggt tctttatcat tagctataac taatgtgtac ttaaaaatag gaatacatga
     2341 gtaaaactca tgcataagaa atactaattt ctaaagaaaa agtaattctt tatcgatgtc
     2401 ctgccccact tgcattggtg ggattatcaa tcaatgtgtt ttctctaatg caaaaagacg
     2461 ctttctatca aaacaagata gaaagcgtct ttttagtatg tccgagtcat aaaaacaact
     2521 cgtagcttat caaattactt attcaaatgt ttactgtcat ctttatacac aaagtatttg
     2581 aagtattttc cttcagtctt catgttctta taaaagtcag cgataattgt tgcattactt
     2641 tctgcccact taatatctgt agcatattga tgttctcctg gattttttgg attccatctc
     2701 atactataca atgtattttg atctgtgctt gataagaagt gcttatgaat gaaatcagca
     2761 ccgcctgaaa tagctttttc aggtgtatcc caaccatgct ttttagcata ttctgcacct
     2821 gttttaattg ggtctttatc aagggctcct actccataga aattgtagta ctttttgcca
     2881 tcaatttcga ctccattagc taattcactt ttaactgcgc cagtttctaa taatgcatgt
     2941 gaaattaaat aaacttcgtt aacgtgctta tctttagcag cttttaagaa atcatccgta
     3001 tgtttcaata acgttggtct atctactaac atacgtttaa ttctattttt atcaatccct
     3061 tgatactttg ataaatctaa aaattgatat ttttgctttt cattatcgat aaaagtaccg
     3121 ctatccattg cacttttaat ttcagttgca gatgcatctc tccatgcatc attcttttta
     3181 tttgatacct gttgactcgt ataattattt atttgtttct ttgctgcatc gtttaatgta
     3241 acatttaact tttcaatctt aatgtcggat ttaacatgtt tgaaaaatat ctgatcggat
     3301 atcattgaga aaaataaaaa tgagacaaca gcaaatatga caacaagtcc tattattcca
     3361 aaaatagaac ctttcttgtg tttattcata tccacacctc ttaggtcatt gttgttatac
     3421 attcagtttg actacgataa taaagtttaa cattattaaa accgtttaac aattttatct
     3481 atgcattgtt acatattcat aaataaacag taatgtaaat gaaacgtttt aaaccattta
     3541 tcaacattac tttacgttga tctacgaatc ataaatgttt tatctttttg tataaccttt
     3601 gaaaaccttt atatcaataa tcttattatt cacgtattaa aaaacaaaaa attcttttgt
     3661 tcgtttactt agtttctttt ttataatcca tcataataaa acttgctgca tttgatctat
     3721 ttttataata gtactgattt aaatcaatcg tgccttcaat ttgaccatca cgatacatca
     3781 tttcattagt gaagttattt atcatcacga aatcagcgcg atctcttata acatctaaat
     3841 cgtcgtttcg tgcaaaaaaa tgttctaaat ttaaatgtgc gtaatccatt gttacctccc
     3901 gattacaaat tttgttctat ctactattta gcaaagtaat aatacatcat atcacattaa
     3961 attaattact tatgtcttat atttaacact taatttagcg attacgcaaa tgaatgattt
     4021 ttctaaatat tttattctaa ctttatccgg aaatatttgt tat
//
LOCUS       DAKTIP010000201         4057 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.204, whole genome shotgun sequence.
ACCESSION   DAKTIP010000201 DAKTIP010000000
VERSION     DAKTIP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.204"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..2426
                     /locus_tag="O0305_002406"
     CDS             <1..2426
                     /locus_tag="O0305_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0275516.1"
                     /translation="VTNNNITFSNLAEKINQEFEINDITKVKVYIHNLVSKEIIYSTI
                     RPPLSYSDNLNYILNKLSLHNDDFVKKIREIQKLILAYEKTEIGFGEELYKDIIHHMK
                     ALFKCKNYLQIDTKIDMINNYLHQDIATNISEAAYLLWLLSRNNIGFTDLKVLHNRFI
                     EKYGFEQLVNIKDLISDITGFGTTIFQEEETDGNNIVMLKQKFLHALRNNDEIVINEK
                     DVESLINDNEINHYHAPMSADVYAELYLGRFYNQYNELIVISPLTASFNAGATFGRFH
                     HLIDTETLAKLEHEKGHYYQKMICDDNVEMISINNIPKYPRNHNVLTNHDSYEYSLNL
                     GSSNSYSKYELTLDDIYVGATFNKLYLYSSQLNKRVLFESNNMYNFLKECNLYRLLRE
                     ISMESVKCIEPMNDVSIDSFSYSPRIRYKNVILKPAYWKINEMVLPLPKNEEWDQQFL
                     KYQEQFNIPNIVNLVYGDNKLLLNLSLANHRYLLMKEYKKHKRVRLVESFLPQSKNDH
                     VYEIVTPIYKKSSYRGPEIEIPKYKNTDIEYDKDWFALHIHIDKPSQDTFIIDNLYPF
                     VKHLKDKGDIDQYFLMRYIKQGDILKLRLFRNDENYAEIYSILKNWLPHVRQTTEVSD
                     YEFVSYEPEFFRYGGKNTINEIEAFFEYDTNLAVNIIENDFKFDRPYIVAISIMYLFE
                     MFSISNEERMEIVNNYVPTSFKSKDIRPFKNELVTICNPANNFEYMAKHYSGIYRILK
                     DGNQILSKLNEGLKKTLTTKRSRIIGSLIHMRCNRIFGIDKDQETFVLSIVKEIVKTQ
                     KHWCGDKND"
     gene            2419..3663
                     /locus_tag="O0305_002407"
     CDS             2419..3663
                     /locus_tag="O0305_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HCY0275517.1"
                     /translation="MIDMNLILKKKFQEISEVDDFISKASTETDYFEPSTLSHGIPGI
                     ILFLDAYQKVFDINTEQIVHKYIMKLAPYLQSGQYNNSLFGGLSGIAFSMDLASQNGK
                     NYQNILNNIDGHIVNEIENKFDQILQEPLNPLNYDTVSGLAGIGRYLLNRVDENEFNV
                     KALKSILVYFKDIQYSKNSWVVPQESQFLESDKNYFTEGNINLGLAHGVLGPMSLFAL
                     CVIKGITIENHQHILKDMYKFIMDEKFCNHERWLQRYDLISERNHFNFIRNGWCYGNT
                     GVMTTLFLIGQALQDDEIIKMSKKVMLQVVNDKDENLISPTICHGLSSQILMLTIMNL
                     NFELNEVSDYITVLINKLISHYKEDYLVNFIDINENKQDVFKSRKVGLLEGELGVYLT
                     LMTLKNTKILNEKNWTNAFLIS"
     gene            3679..>4057
                     /locus_tag="O0305_002408"
     CDS             3679..>4057
                     /locus_tag="O0305_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HCY0275518.1"
                     /translation="MGENVLICLCGSVNSINISHYIIELKSKFDEVNVIASTNGRKFI
                     NGEILKQFCDNYYDEFEDPFLNHVDIANKHDKIIILPATSNTINKIANGICDNLLLTI
                     CHTAFEKLSIFPNMNLRMWENPVT"
BASE COUNT         1558 a          490 c          651 g         1358 t
ORIGIN      
        1 ctgtgacgaa taataatatt actttttcaa atttagctga aaagataaat caagaatttg
       61 aaattaatga tattactaaa gtgaaagtat atatccataa tcttgtctca aaggaaatta
      121 tatattcaac aatcagacca ccgttatcat atagcgataa tttaaattat attttaaata
      181 aactttcttt acataatgat gattttgtga aaaaaattag agaaatccag aaattgatat
      241 tagcttatga aaaaactgaa attggatttg gcgaggaatt atataaagac attattcatc
      301 atatgaaggc actattcaaa tgtaaaaact atcttcaaat tgataccaaa attgacatga
      361 ttaacaatta tttacatcaa gatatagcaa caaatatatc tgaagcagcg tacttattgt
      421 ggcttttatc aagaaataat attggattca cagatttgaa agttcttcac aaccgtttta
      481 ttgaaaagta tggatttgaa caactcgtga atattaaaga tttgatttca gatataacag
      541 gtttcgggac aacaattttt caagaagaag aaaccgatgg aaataatatt gtaatgctta
      601 agcaaaaatt tttacatgcc ttgagaaata acgatgagat agtcataaat gaaaaagatg
      661 tagagtcatt gattaacgat aatgaaatca atcattatca tgcgccaatg agtgcagatg
      721 tttatgctga actttattta ggacgttttt acaatcagta caatgagctc attgttatta
      781 gtcctttaac ggcgtctttt aatgctggcg ctacatttgg gcgatttcat catttaattg
      841 atactgaaac tttagcaaaa ttagaacatg aaaaaggaca ttattatcag aagatgatat
      901 gtgatgacaa tgtagaaatg atttctataa ataacatacc gaaatatccg agaaatcata
      961 atgtattaac taatcatgac tcatacgaat attcattgaa tttaggaagt agtaatagtt
     1021 attcaaagta tgagcttacc ttagatgata tttatgttgg tgctaccttt aacaaattat
     1081 atttatattc tagccaacta aataaaaggg tactatttga atcaaacaat atgtataact
     1141 ttttaaaaga atgtaattta tatcgattgt tacgagaaat ttcaatggaa tctgtgaaat
     1201 gcattgaacc tatgaatgat gtgagtattg attcatttag ttattcacca cgaatcagat
     1261 ataaaaatgt cattcttaaa ccagcatatt ggaaaattaa cgagatggta ttacctttac
     1321 ctaaaaatga agaatgggat caacaatttt tgaaatatca agaacaattc aatataccta
     1381 atatcgtgaa tttagtttat ggagataaca agcttctact aaatttatca ttagctaatc
     1441 atagatattt attgatgaaa gaatataaga agcataaacg tgtacgttta gtagaatcat
     1501 ttttacctca atcaaagaat gatcatgtgt atgaaattgt tacaccaatt tataaaaaat
     1561 catcttatcg aggacccgaa atcgaaattc caaaatataa aaatacagat attgaatatg
     1621 acaaagactg gtttgcatta catattcata tcgataagcc atcgcaagat acatttatca
     1681 tcgacaattt gtatccattt gtaaaacatc ttaaagataa gggagatata gatcaatatt
     1741 ttttaatgcg ttatatcaaa caaggtgaca ttttaaagtt gaggttattc aggaatgacg
     1801 aaaattacgc agaaatatac agcatcttga agaattggtt accacatgtt cgacaaacga
     1861 cagaagtttc agattatgaa tttgtatcat acgagccaga attttttaga tatggtggaa
     1921 agaacacgat taatgaaata gaagccttct ttgaatatga cacgaattta gctgtcaata
     1981 ttattgaaaa tgattttaag tttgatcgac catatattgt tgctatttca ataatgtatt
     2041 tatttgagat gttttcaatt tctaatgaag agcgtatgga aattgttaat aactatgtac
     2101 ctacatcatt taaaagtaaa gatattagac cttttaaaaa tgaacttgta actatatgta
     2161 atcctgctaa taattttgaa tatatggcta aacactatag tggtatttat cgaattttaa
     2221 aagatggcaa ccaaattttg agcaaattaa atgaagggct taaaaaaact ttaactacta
     2281 aaaggtcaag gattattggc agcttaattc atatgcgctg caatcgaata tttggcattg
     2341 ataaggatca agaaacattt gttttatcta ttgtaaaaga aattgttaaa actcaaaagc
     2401 attggtgtgg tgacaaaaat gattgatatg aatctaatat tgaagaagaa gtttcaagag
     2461 atttcagaag tagatgattt catttcaaaa gcatctactg aaaccgatta ttttgaacct
     2521 tcaactttat cacatggtat accaggtatc attttgtttt tagatgctta tcaaaaagtt
     2581 ttcgatatta atactgagca aattgtgcat aagtacatca tgaaattagc accatattta
     2641 cagagtggtc aatataacaa ttctttgttt ggtggacttt caggtattgc attttcaatg
     2701 gatttagcat cgcaaaatgg caaaaattat caaaatattt taaataatat tgatggtcac
     2761 attgtaaatg aaatagaaaa taaatttgat caaatattac aagaaccatt aaatccatta
     2821 aattatgata ctgtcagtgg attagctggg atagggagat atttgctaaa tagagtagat
     2881 gagaatgaat ttaatgttaa agcattaaaa agcatattag tatactttaa agatattcaa
     2941 tattctaaaa atagctgggt agtcccacaa gaaagtcaat ttttagagtc tgataaaaat
     3001 tattttactg aaggtaatat caatcttggc cttgcacatg gagtgctagg accgatgtct
     3061 ttatttgcat tatgcgtgat taaaggaatt acgattgaaa atcatcagca catattaaaa
     3121 gacatgtaca aatttatcat ggacgaaaaa ttttgtaacc acgaaagatg gttgcagcgt
     3181 tacgatttaa tttctgaacg taatcatttc aattttattc ggaatggttg gtgttatggc
     3241 aatacgggtg taatgacgac gttgttttta atcggccaag cattacaaga tgatgaaata
     3301 attaaaatgt ctaaaaaagt gatgctacaa gtagtaaatg ataaagatga aaatttaata
     3361 agtccaacta tttgtcatgg attgtcatca caaatattaa tgttaacaat tatgaatttg
     3421 aattttgaat taaatgaagt gtctgattat atcactgtat taataaataa actgatttct
     3481 cattataagg aagattatct ggtgaatttt atagacatta atgaaaataa gcaagatgta
     3541 tttaaaagta ggaaagttgg ccttttagaa ggtgaattag gggtctatct aacattgatg
     3601 acactgaaaa atacaaaaat tctcaatgaa aaaaattgga ccaatgcgtt tttaattagc
     3661 tgaatggagg cgtatatcat gggagaaaat gtattgattt gtttgtgtgg ctcagttaat
     3721 agtatcaata ttagccacta tattattgaa ttaaaatcaa aatttgatga agtaaatgta
     3781 atcgcgtcaa caaacggtag aaagtttatt aatggtgaaa tattaaagca attttgcgat
     3841 aactattatg acgaatttga agaccctttt ttaaatcatg tagatatagc aaataaacat
     3901 gacaagatta ttattttacc tgcgacttct aatacgatta ataaaattgc aaatggtata
     3961 tgtgacaatt tattattaac tatttgtcat acagcttttg aaaaactttc tatatttcca
     4021 aatatgaatt tacgaatgtg ggaaaatcca gttactc
//
LOCUS       DAKTIP010000202         4024 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.205, whole genome shotgun sequence.
ACCESSION   DAKTIP010000202 DAKTIP010000000
VERSION     DAKTIP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.205"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            342..1277
                     /gene="lukE"
                     /locus_tag="O0305_002409"
     CDS             342..1277
                     /gene="lukE"
                     /locus_tag="O0305_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCY0275519.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEVNWKTHEIKVKGHN"
     gene            1279..2262
                     /gene="lukD"
                     /locus_tag="O0305_002410"
     CDS             1279..2262
                     /gene="lukD"
                     /locus_tag="O0305_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCY0275520.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            2504..2647
                     /locus_tag="O0305_002411"
     CDS             2504..2647
                     /locus_tag="O0305_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0275521.1"
                     /translation="MEKVLDLDVQVKGNNNTNDSAGDERITSHLFCSFGCEKTGSFNS
                     FCC"
     gene            3384..3527
                     /locus_tag="O0305_002412"
     CDS             3384..3527
                     /locus_tag="O0305_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HCY0275522.1"
                     /translation="MEKVLDLDVQVKANNNSNDSAGDERITSHSLCTPGCAKTGSFNS
                     FCC"
     gene            3592..>4024
                     /locus_tag="O0305_002413"
     CDS             3592..>4024
                     /locus_tag="O0305_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HCY0275523.1"
                     /translation="MNTIYEPSSICMIRTPLLSVEFFNLFLNTEQIKYSDLQLNAQMK
                     ESILTTTFNLYCTLQEINFDGDNKKVRDAKESLLKYLIRMSTRPTPFGLLSGINLGHF
                     VNEPTRLKVGNSIQKYVKVDGEWLYKLVSYIESIDEYYQNLK"
BASE COUNT         1555 a          509 c          657 g         1303 t
ORIGIN      
        1 ggacaatagt taataatcag ttaattttaa attcaattat agattatttg tgataataca
       61 aacattattt ttgaagattt gattaaagtc gtttttattt ttacagagaa aagattgagt
      121 atcggtaatt ttatcatatt gttcgaatat aaagggaact atttaaaggt aatgaaggtt
      181 gcgccaaaat gaccggtttt cagcagcata aatgaataaa ttgtgtcatt tttaatattt
      241 tatagcattt tattttttaa tgaacagtta attttataaa taagtgaaat aatctagaat
      301 aaatttgtat taaaaattta tatagaaaga aagtgaaact tatgtttaag aaaaaaatgt
      361 tagctgcaac tttgtcagta ggactgattg cacctttagc atctccgatt caagaatcta
      421 gagcaaatac taatattgaa aatattggtg atggtgctga agtaatcaaa cgtacggagg
      481 atgtaagtag taagaaatgg ggcgttactc aaaatgtcca attcgacttt gtaaaagata
      541 aaaaatataa caaagacgct ttaattgtta aaatgcaagg ttttattaat tccagaactt
      601 cattttcaga tgtgaagggt agtggatatg aattaactaa acgaatgatt tggccattcc
      661 aatataatat aggactgacg actaaagatc caaatgttag cttaatcaat taccttccta
      721 aaaacaaaat agaaactact gatgttggtc aaacattagg atataacatt ggaggtaatt
      781 tccagtcagc accatctata ggtggcaatg gctcatttaa ttattctaaa acaattagtt
      841 atacccaaaa gagttatgtc agtgaagtag acaagcaaaa ctcaaaatct gttaaatggg
      901 gtgttaaagc aaacgaattt gttacgcctg atggaaaaaa atctgcgcat gatagatatt
      961 tattcgtaca aagtccaaat ggtccaacag gttcagcaag agaatatttt gctcctgata
     1021 atcaattgcc acctttagtt caaagtggct ttaatccatc gtttatcact acactatcac
     1081 atgaaaaagg ttcaagtgat acgagtgaat ttgaaatttc atatggtaga aacttagata
     1141 ttacatatgc gactttattc cctagaactg gtatttacgc agaaagaaag cataatgcat
     1201 ttgtaaatag aaactttgta gttagatatg aagttaattg gaaaacacac gaaattaaag
     1261 tgaaaggaca taattaatat gaaaatgaaa aaattagtca aatcatcagt tgcttcatca
     1321 attgcactgc ttttgctatc gaatacagtt gatgcagctc aacatatcac acctgtaagc
     1381 gagaaaaaag tagatgacaa aatcacttta tacaaaacaa cagcaacatc tgataatgat
     1441 aaattgaata tttctcaaat tttaacgttt aatttcatta aggataaaag ttatgacaaa
     1501 gatacgttag tacttaaggc agccggaaac attaattcag gttataaaaa gcctaatcca
     1561 aaagattaca attactcaca gttttattgg ggcggtaagt ataatgtttc ggttagttca
     1621 gaatcaaatg atgctgtaaa tgttgttgac tatgcaccta aaaatcaaaa tgaagaattc
     1681 caagttcaac aaacattagg ttattcttat ggcggagata ttaatatatc taatggctta
     1741 tcaggtggat taaatggatc aaaatcattt tcagaaacga taaattataa acaagaaagt
     1801 tacagaacta cgattgatag aaaaacaaat cataaatcaa ttggctgggg tgttgaggcg
     1861 cacaaaatta tgaataatgg ttggggacca tatggtagag atagttatga cccaacatat
     1921 ggtaatgaac tgtttttagg cggtagacaa agtagttcaa atgctggtca aaatttcttg
     1981 ccaacacatc aaatgccttt attggcgcgt ggtaacttta acccagaatt tataagcgta
     2041 ctttctcata aacaaaatga tacaaaaaaa tctaaaatca aagtaactta ccaaagagaa
     2101 atggatagat atactaatca atggaatcga ctacactggg ttggtaataa ctacaaaaat
     2161 caaaatacag taacgtttac atctacttat gaagttgact ggcaaaacca tactgttaaa
     2221 ttaatcggta cggattctaa agaaactaat cctggagtat aactgttaga aatagaggaa
     2281 gttaaacccg aaatttaaga gttaaacaaa aaatattcac aacatattaa aaaaagaagt
     2341 tacattattt gtttaaacta acaatagtac cataaaatta atgacgattt acaaattaat
     2401 aacaaataat tattattaaa ttcattgatt tattgtgtgg taatgattaa tatgacaatt
     2461 gaagtgggtc ttaacttcaa cttttaaata gggggtttta tctttggaaa aagttcttga
     2521 tttagacgtg caagttaaag gaaataacaa cactaatgat tcagcgggtg acgaaagaat
     2581 aactagccat cttttttgta gctttggttg tgaaaagacg ggtagtttta acagcttctg
     2641 ttgttaagga tacagcaaat agagcctgaa agggctctat ttgtcttttt aaatatatta
     2701 agtgctaaaa aacggaaaca gtttattaat cgccaggtat ttttataatt agccttctat
     2761 attatcagtt gagtgtttca ataaattctg actttggtga tggaaagtta tacttttagt
     2821 tgtaatatag ttttcatgac ttcctgttaa ataagttagg attatgtaat tagcctctat
     2881 ctttacatat aagtaatgac aagctttggt tattatattg accctcttag aacatgtcat
     2941 ttaagaaaat aatcaagtca ttgaaattat atgtttatat cctcatcttt aaaataactt
     3001 caattattat ttgctaaatt ttgtataaat agaacaaaac tgcataaata tctattaatt
     3061 aatctcaagc agccatctcc aaattcgtaa tagtattgat agataaaaat atctaagtat
     3121 ttgtataaat gaattgatta atctattaaa aaattcatca ataaatttaa aattatgaac
     3181 ttagatgcaa aattattagt gaaaataaat agtaataaaa aaacacaaaa atctaattat
     3241 caaaatagta ttactaaatt aataagtgtt tacaaaaaat tcacaaaata taattatata
     3301 atttattgat ttgttgtgtg aaaagggata atatgacaat tgaagtaggc cttaacttca
     3361 actaataata ggaggtttta tctatggaaa aagttcttga tttagacgtg caagttaaag
     3421 caaacaataa ctcaaatgat tcagcaggtg acgaacgtat tacaagtcat agtttatgta
     3481 ctcctggttg tgctaagact ggtagtttta atagcttctg ctgttaagat tacagcaaat
     3541 agagcctgaa agggctctat ttgtctattc aaatatgtga ggtgcttaca aatgaacact
     3601 atttacgaac cgtcaagtat ttgtatgatt agaacacctt tattatcagt tgaatttttc
     3661 aatttatttt taaatactga acaaattaaa tatagcgact tacaattaaa tgctcagatg
     3721 aaagaatcaa tcctaacaac aacatttaac ttatattgta ctttacaaga aataaatttt
     3781 gatggcgata acaagaaggt tagagatgct aaagaaagtt tattaaaata tttaattaga
     3841 atgtcaacac gtccaactcc atttggatta ttaagtggta ttaatttggg gcattttgta
     3901 aatgaaccaa cacgtcttaa agttggaaat agtattcaaa aatatgtgaa agtcgatggc
     3961 gaatggttgt ataaactggt tagttatatt gagagtattg atgaatatta tcaaaatttg
     4021 aaag
//
LOCUS       DAKTIP010000203         4014 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAKTIP010000203 DAKTIP010000000
VERSION     DAKTIP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.206"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..176)
                     /locus_tag="O0305_002414"
     CDS             complement(<1..176)
                     /locus_tag="O0305_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCY0275524.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFS"
     gene            complement(385..534)
                     /gene="rpmG"
                     /locus_tag="O0305_002415"
     CDS             complement(385..534)
                     /gene="rpmG"
                     /locus_tag="O0305_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0275525.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            complement(647..2722)
                     /locus_tag="O0305_002416"
     CDS             complement(647..2722)
                     /locus_tag="O0305_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCY0275526.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIIFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVTINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     NDKNKDKDK"
     gene            complement(2843..3442)
                     /locus_tag="O0305_002417"
     CDS             complement(2843..3442)
                     /locus_tag="O0305_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0275527.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(3719..>4014)
                     /locus_tag="O0305_002418"
     CDS             complement(3719..>4014)
                     /locus_tag="O0305_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCY0275528.1"
                     /translation="IQQVMDENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIA
                     CTNHHHHHFICEKCGDTKVIDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
BASE COUNT         1178 a          723 c          551 g         1562 t
ORIGIN      
        1 gaaaaagtat ctacttcatg attaaaagaa agaactagcg caattgcgtt tgcttctttg
       61 tattcttcag ttgcaaaaaa ttgatttctt aaccatgtgt ctgcttttcg cttttcagct
      121 ttattaaaat tcttcatttt atgtaaaatg tattttctaa tctcattttt agtcacatta
      181 tcacccactt tattcaattc tattatatcg ttaataacaa tacatgtcat tatgttgtca
      241 tgatgaatta tgcgacaatt ctaaaattta ttatttcaat tgaaaatctt ttatcatttc
      301 atacgtgtgc ataattaaaa aggtgaaacc tcttattttg agatttcacc ttttttattg
      361 ttaatttaaa tttaatttta atgattattt tgtttcacgg tgtaaagttt gtttgttttc
      421 acgtgaacag aatttcttca tttcaacacg ttctggatta tttcttttgt tcttagttgt
      481 aatgtagttt ctgtcaccac attccgtaca agctaaagtt acgtttacgc gcatggttat
      541 tcctccttat catttctaaa tacgacttta ttattatacc aacatctata atatttgcaa
      601 agttaaaatt ttacaaccat gcgctacaca atcgtcaggt taaattttat ttgtctttgt
      661 ctttattttt atcattttta cctaactgct taaagtagta gttaattaca tctctaccta
      721 agtctccacc tgttaaccat ggtggtggta caggctgatt tgtatataca attgaaaacg
      781 ctaattttgg atcatcaatt ggcgcgtatc ctatataagt agagttaact cttggctctc
      841 cgttttggaa cacttctgcg gtacccgttt taccagcagt aggtactact gtatctttaa
      901 aactaacata tccagtacca tctttatcat taaatgccat tttgaatcct tcttgaattt
      961 gtttgatttc cttttcagta ttattaacct tgttcaagac agtgccatta attttcttct
     1021 tgagtggacc aacctcatct ttattagttg attcatgaat cgttaatcca atgtgtggct
     1081 gtattctata accatcattc gctatagttg aaacatattg tgataattgt aatggtgtat
     1141 aggtatcata ttgaccaatt gataaatcta gataattacc tggattattt gttaatggtt
     1201 cgatttgacc tcttgtttca tttggtaaat ctatccctgt tttcacacct aagcctactt
     1261 gatttaatcc tcttcttagc ttttgggcag gtgaacttat gtctgaaggt aaagccatac
     1321 cagaataata agggtctccc gctaatttta atgctgtttt aaacatatat acgtttgatg
     1381 aatgcatcaa agcttgctta tcattaatag ttacatgccc gtttttattg aagtatgatc
     1441 gttttgtcaa accaccttgg aaatgtaatg gttcatcgac cattgtttct ccaactttga
     1501 tagctttatt ctgataaccg gctaataatg ttccaccttt tacagaagat ccaaccgcaa
     1561 attgagaagt aaacgtacca atgtcataat cagtcatttt accactctta ttaatctgct
     1621 ttccggcaag cgcaagaatg tctccatttt taggattttg tacaaccatc attgcattat
     1681 ccatatcttt ggcaccttga ctgcgaagct tcttaatttg tttatctaat aatgcttcta
     1741 cttctttttg aagatctata tcgatcgtta atttcaaatc ttgaccgcga gcgccaggat
     1801 ttaacacttc tgaagatgta actttaccag atttgtccgt tgtgtatttc atttctttct
     1861 tcttaccacg caatacatct tcatattgat attctaggta agattttcca acacgatcat
     1921 tgcgtgaata tcctttggat aagtaatgtt ctgtcaattc ttttggaata ccttcagcag
     1981 gtgtcgatac atctccgaat atacctctta aagtatcgcc atatggatat tttctatccc
     2041 aatccataga cgtgttaaca cctggtaatt tggaaagttg ctgagaaact gctgcatact
     2101 ctttttcact gacatcttca ttttttatca tttgtggatc taaaactgtt cctgcattca
     2161 tctctcgaaa aatagctaaa acttgtaaat ctttagaaga caattcatct aattgtgatt
     2221 ttccgatttt cgataacagt tgtttatcat attgatcttg tttaatactt ccatctgcta
     2281 acatagcttg ttcttttgtc atcattgctt ttgctttttt aggatgcaac tgaatccaga
     2341 aatctttctt atcacgttct gtaattttct tagtatccat cttgattagc tttgataact
     2401 tttcagccgt atccaacatt tccgattgtg ttgtttttcg acccctagta tatgtaatag
     2461 ccattttaga agcattatca actaaaactt tcccatttct gtctaaaata cgacctcttg
     2521 gcacagactc attcactgta atgttttcat cattttttat aatttgttta taatgtgagc
     2581 cttgtgcgat ttgtaaataa cctaaacgta gtactagtac tgcaaaaata aatataatca
     2641 caccaaatat aaagttaatt ctcttgttaa ttgtattttg aacgatttca tcatttgatt
     2701 tttcttttag tctttttaac aaaactacct acctctattc aaagtctttc actttaaatc
     2761 atatatgaat ttagaaatta tttctatctt tttgacaaaa aaataacggt ctcatttaag
     2821 agaccgaaca agtaatcata ctttattttg ttgcattata taattcgtca actttttccc
     2881 agttaactac attccaaaat gcgccaatgt agtcagggcg tttgttttga tattttaggt
     2941 aataagcgtg ttcccatacg tctaaaccta aaataggtgt tttaccctca gttaatggat
     3001 tatcttggtt tggtgtagtc acaatttcta actggccatt gtttacgact aaccaagccc
     3061 aacctgaacc aaagcgtgca gctgctttgt cagcaaattc ttttttaaat tcttctaaag
     3121 aaccccattg ttctttaatt ttttctacta cagtaccttt ttcttctgag tttggtgaaa
     3181 gtaactccca gaataatgaa tggtttaaat gtccaccgcc attattacgt acagcagttt
     3241 ggatgttagc tggtacactg tctaaattag caacaatttc ttcaatagat ttagattcta
     3301 aatctgtacc ttctactgca gcatttaatt tcgtaacata cgtgttatga tgtctgtcat
     3361 ggtgaatttc catagtttct ttgtcaaaat gtggttctaa tgcatcaaat gcgtatggta
     3421 attttggtaa ttcaaaagcc ataaataatc atcctcctaa aatgtctgta agtaaataat
     3481 aacaagcagt gactggttca acaaagaatt tgcttaaatt ctactactta ttattttctc
     3541 tactcattta atataactca aatcaaaaat aattaaacat tttgtatata aaaagttaac
     3601 agatttgcca taaaatcata cgaacggagt atgaaatgaa cctttatctt ctataatata
     3661 aagaaatgag atttatgcat acatctgacc aaatgtgcat aaatctcatt tctttatatt
     3721 aatcttggca agactcacat acaccataaa cttcaagttt gtgtttgtga atattaacac
     3781 caggtagtga taattttatc tgatctattg gacaataatc tattaccttt gtatctccac
     3841 acttttcaca gataaaatga tgatgatgat ggtttgtaca agcgattcta aacttcattt
     3901 caccatcaag ttctgtattt tcaataattc ctaaatcttt aaataagtgc aggtttctat
     3961 atattgtgtc gaatgaaatt ccaggataat tttcatccat aacttgttgt atat
//
LOCUS       DAKTIP010000204         3969 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAKTIP010000204 DAKTIP010000000
VERSION     DAKTIP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.207"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(270..2078)
                     /gene="pepF"
                     /locus_tag="O0305_002419"
     CDS             complement(270..2078)
                     /gene="pepF"
                     /locus_tag="O0305_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCY0275529.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2126..3111)
                     /locus_tag="O0305_002420"
                     /pseudo
     CDS             complement(2126..3111)
                     /locus_tag="O0305_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
     gene            complement(3232..3951)
                     /gene="mecA"
                     /locus_tag="O0305_002421"
     CDS             complement(3232..3951)
                     /gene="mecA"
                     /locus_tag="O0305_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCY0275530.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         1227 a          704 c          511 g         1527 t
ORIGIN      
        1 taatgtataa aaagtgcata tttaatacat ttttatacaa cttcacttcc ctcttattaa
       61 aaaagaacat aaaaaaacgt cgtgtctgaa tacacgacgc taaacaaagg ggatgggaga
      121 aatttttcac ttcaaacaaa ggggtatgtt tgttatgtga ttaatttcat gttcataata
      181 taacatgcta tctctaccat ttcaaccttt taatagtact taatttaaat tgtcacaatc
      241 tgttcataat gtaagcgaat acaatagtac taagctttca ttaatttttc aaaagcgttc
      301 aatttttgtt caaaaacttc acaagcttgt tcaattggtt caggtgttgt catatctaca
      361 ccagcatttt ttaatatctc aattggataa tttgagctac cttttttcaa gaattcatta
      421 atatatctat ctactgctgg cttaccttct gttaaaattt gatgacttaa gctttgagct
      481 gcactgtaac cagttgcgta ttgatataca taataattca tatagaagtg tggaatacgt
      541 gaccattcct tactaatatc ttcatcagtt tctacagaat caccgaagta taatttattt
      601 aatttggcat attcttcatt cattctagtt ggcgttaatg gttcacctgc ttcttcaatt
      661 gcatgaattt tatgctcaaa ttctgcgaac attgtttgtc ggaataatgt agctctgaaa
      721 cgttctaatt cttggtttaa taataataag cgtttttcat catcaagatg tttatccata
      781 taatcactta aaagtgcttc gttacaagtt gatgcaactt cagcgacaaa aatagtgtag
      841 tcactagaat ttgacggttg gaattttcta ctgaagtaac tatgtgctga atgcccaaat
      901 tcatgaacta atgtgtataa gtctgaaata gtattagacc agttaagtag aataaatgga
      961 ttagttaaat gtgcacctga tgaatagcca cctgaacgtt tacctttatt ctcatagaca
     1021 tcgacccaac gattgtttaa gccttcttta actacattta aatattcttc acccattggt
     1081 tctaatgctt ttaacatcca ctctttagct tcttcataag gcatttcaaa cttaatatct
     1141 ttaattaatg gtgtatataa atcatacatt ttcaagtcat ctaaacctag caattctttg
     1201 cgcaattcag tatatctatg tagcaatggt aaatatttat gtacagtttt tactagattg
     1261 tcatatacat tttctggaat atgattatta ctcaatgctt tttctcttgc agttttgtaa
     1321 ttgtgtgtac gagcattaaa tacatttttc ttcacttcac ctgctagcgt agcgccaagc
     1381 gtattattat gagcaccata tgctttatat acatttctaa aagcactttc tcttagtttg
     1441 cgatcatctg tttctaaata cttaataaat gtaccttgtg ttaacgggtg tgcatttcca
     1501 tctttatcta tcgcatcttc aaatactaaa tcagcgttgc taaacatacc gtatacattt
     1561 gatggcgttg ataacgcgtc ctgcgcttct gttaataact tttcagtttc agcatctaaa
     1621 atatgaggac gtttttcatt aatcaacttc aaatcaaatg cgaatttttg taatttatca
     1681 tatgaattta caaatgattg aattttatct tcatcaattt gtaaaatctc tggcactaag
     1741 aaactccatg ccgagctaaa tttaataatt aattgatgtg ctcttgactc cataccagta
     1801 tacttgtcgt tcgttgtatc ttggtcttgt tttaggtgcg cgtatacata tactttttct
     1861 aatttagtac ctaatgtatc ttctaattct aacgcattgt ataatgtctc agcactatca
     1921 ccaatgtgtc ctttaaattg ttcttctttg cctaactcat tttcaacttc tttaaatgca
     1981 gcctcaaaag cttcatcatc tttgaaaatt gttgttaagt cccatgtata ttcaggatat
     2041 ttacgttcct gttcttctct cgataattgt tgactcatat gtagtaaacc tcctaataaa
     2101 atataattga tataattttc tcattttaga acaaaatttg cacgtcatta cctttatctg
     2161 aacaaatatt taaataattg ttaataagtt tttctataat tgctgtttta ctttctagag
     2221 caacaataaa ttttttcagt ttaaaataat taagattgtc atttactaat ttgctttttc
     2281 cattctttaa ccataaatca acttgtaatt gccactcaat aggatgattt ttaatataaa
     2341 tttgctctgg aaaaatataa ccataattgt gcactacttc ttgatcagtt aactgtaatt
     2401 gatacattgc acttaaagtg ggttctaaaa cagaattttg ccagcgacaa tatttgatat
     2461 attgattaat tgcgaacttt gataatttat aaacagcatg ataatcacaa ggcgcctcat
     2521 taaacaactc actaattttg gcatttcttt tttcagcgac atatttgtgc ccgcctaaat
     2581 attgtatttg ttgaaacatc attatttgtt ttttctctaa attgaacgta tgaagttttc
     2641 gagtgaatgg attaataaac atactttgaa aatgattgaa cttcacttta ttatgacaat
     2701 aatcaacatc atttaataac caacttactt tatatcccat tttctttaaa ccttccgttc
     2761 gttgaagaat ctgtttataa ggaattggcg aatactgtag ctcaataaca tatttattat
     2821 taatcaaaat atccggaatt tgttttattt cttttaaaat ggctctatct ctactttaca
     2881 tccttgttgc tgtatcatct gtgctaaaat caatttactt ttataatgtt taatcgtttc
     2941 attattaaaa catttttgtt ccgctaaatg tttatgtgca aaatgactaa ttactttgag
     3001 cccacgcttt aaaataactt gcttgccaca caccggacaa aaatattgtg tctttctcaa
     3061 tgcagtagtt gctaatacgc gttccttttc ttcatttaaa gctactaaca tatcttcacc
     3121 tcaaaatatt acacgtaagt ttaactacta ttactttttc agcttttgaa atcaataaaa
     3181 aaagagactc caaagagtct cctttcatag tgcgttgctt aattaatatc gttattcagt
     3241 tgtctctgga aaataacgtc gaacttgagc tgttacgtta tgactcataa ttattttagc
     3301 atagtcattt aaataaactt ctgttctgtc tgttggataa gcaaattcaa gcaattgact
     3361 gtaactatca ttaatgactt cttgatcaac atgactatca aaatatacag cataataata
     3421 agtaccatca accatatata acaaatcttc aaactctgta gttattggat tgctatgata
     3481 tgcataatta ataacatctt ctaaatcgtt aaatttcaca atgattgttc ttgtattttt
     3541 acgtgctgaa gacttttgac gtttagaacc ttgagcttct ttttcttttg ttcgttgctc
     3601 gaataattct tctaattgat cttcaccttc taatgtttga gctaacaatt cttgaacttg
     3661 ttcatcaaat tgatcagttg catcatcatc agacatattc atcatatctt catttttaga
     3721 tttagaaatt gtgacttcga cacctttttc aaaggcatgt acttgaatcc ataatggacc
     3781 ttctacaaca aaatcttctt cttcgttaat ttcatccatc attgaccaaa agaattcttc
     3841 gccacgtttg cgatttgtcc ataaatcttc acgactaaat ccacgggcct cgatatcgct
     3901 atatgttata aacaatttta cagttgtatc atctactcgt tctattctca tatcatctca
     3961 ctccttaca
//
LOCUS       DAKTIP010000205         3960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAKTIP010000205 DAKTIP010000000
VERSION     DAKTIP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.208"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            80..1606
                     /gene="vwb"
                     /locus_tag="O0305_002422"
     CDS             80..1606
                     /gene="vwb"
                     /locus_tag="O0305_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCY0275531.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRM
                     LENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDES
                     KRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYD
                     FEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQL
                     AGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTER
                     EKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK
                     "
     gene            1957..2979
                     /gene="emp"
                     /locus_tag="O0305_002423"
     CDS             1957..2979
                     /gene="emp"
                     /locus_tag="O0305_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCY0275532.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKNSPKVNVSNLTDNKNFVASEDKLNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
     gene            3314..3784
                     /locus_tag="O0305_002424"
     CDS             3314..3784
                     /locus_tag="O0305_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275533.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANDTKQFTEIVS
                     EEKVITVEHAQINIFQSNSNSNLMEFNILTMGGKSGAMVGYSEIDSSHFTDRDKRVIR
                     RDHVKEAQSLVENYKDTQSADARMKAKQKVNTLSKPHQNYFNKQIDKVYNGLQR"
BASE COUNT         1639 a          547 c          634 g         1140 t
ORIGIN      
        1 atgttatata atttggcttg gcgaaaaaat agggtgtaag gtaggttgtt aattagggaa
       61 aattaaggag aaaatacagt tgaaaaataa attgctagtt ttatcattgg gagcattatg
      121 tgtatcacaa atttgggaaa gtaatcgtgc gagtgcagtg gtttctgggg agaagaatcc
      181 atatgtatct gagtcgttga aactgactaa taataaaaat aaatctagaa cagtagaaga
      241 gtataagaaa agcttggatg atttaatatg gtcctttcca aacttagata atgaaagatt
      301 tgataatcct gaatataaag aagctatgaa aaaatatcaa cagagattta tggctgaaga
      361 tgaggctttg aagaaatttt ttagtgaaga gaaaaaaata aaaaatggaa atactgataa
      421 tttagattat ctaggattat ctcatgaaag atatgaaagt gtatttaata ctttgaaaaa
      481 acaaagtgag gagttcttaa aagaaattga agatataaaa aaagataacc ctgaattgaa
      541 agactttaat gaagaggagc aattaaagtg cgacttagaa ttaaacaaat tagaaaatca
      601 gatattaatg ttaggtaaaa cattttatca aaactataga gatgatgttg aaagtttata
      661 tagtaagtta gatttaatta tgggatataa agatgaagaa agagcaaata aaaaagcagt
      721 taacaaaagg atgttagaaa ataaaaaaga agacttagaa accataattg atgaattttt
      781 tagtgatata gataaaacaa gacctaataa tattcctgtt ttagaagatg aaaaacaaga
      841 agagaaaaat cataaaaata tggctcaatt aaaatctgac actgaagcag caaaaagtga
      901 tgaatcaaaa agaagcaaga gaagtaaaag aagtttaaat actcaaaatc acaaacctgc
      961 atctcaagaa gtttctgaac aacaaaaagc tgaatatgat aaaagagcag aagaaagaaa
     1021 agcgagattt ttggataatc aaaaaattaa gaaaacacct gtagtgtcat tagaatatga
     1081 ttttgagcat aaacaacgta ttgacaacga aaacgacaag aaacttgtgg tttctgcacc
     1141 aacaaagaaa ccaacatcac cgactacata tactgaaaca acgacacagg taccaatgcc
     1201 tacagttgag cgtcaaactc agcaacaaat tatttataat gcaccaaaac aattggctgg
     1261 attaaatggt gaaagtcatg atttcacaac aacgcatcaa tcaccaacaa cttcaaatca
     1321 cacgcataat aatgttgttg aatttgaaga aacgtctgct ttacctggta gaaaatcagg
     1381 atcactggtt ggtataagtc aaattgattc ttctcatcta actgaacgtg agaagcgtgt
     1441 aattaagcgt gaacacgtta gagaagctca aaagttagtt gataattata aagatacaca
     1501 tagttataaa gaccgaataa atgcacaaca aaaagtaaat actttaagtg aaggtcatca
     1561 aaaacgtttt aataaacaaa tcaataaagt atataatggc aaataattaa tgcatggctg
     1621 caaagcaaat aatgagtttg tcgtaaaaat aacaacattt taaactagca ataaataata
     1681 tcaaagtcat catttcaatg atgcaatcta gtatagtcca cattctaaac aggtgtggac
     1741 tattactttt ttcactttat attacgaaaa aattattatg cttaactatc aatatcaata
     1801 attaatttta agctgaaaaa caataaaaat gttaagacaa cgtttacttc aagttaatta
     1861 ttatactgaa aattctggta tataatgctg ttagtgaata taacagggaa attatattgg
     1921 ttataatatt gagtctatat aaaggagaaa taacagatga aaaagaaatt attagtttta
     1981 actatgagca cgctatttgc tacacaaatt atgaattcaa atcacgctaa agcatcagtg
     2041 acagagagtg ttgacaaaaa atttgtagtt ccagaatcag gaattaataa aattattcca
     2101 gcttacgatg aatttaagaa ttcgccaaaa gtaaatgtta gtaatttaac tgacaataaa
     2161 aactttgtag cttctgaaga taaattgaat aagattgcag attcatcggc agctagtaaa
     2221 attgtagata aaaactttgt cgtaccagaa tcaaagttag gaaacattgt gccagagtac
     2281 aaagaaatca ataatcgcgt gaatgtagca acaaacaatc cagcttcaca acaagttgat
     2341 aagcattttg ttgctaaagg cccagaagta aatagattta ttacgcaaaa caaagtaaac
     2401 caccacttca ttactacgca aacccactac aagaaagtta ttacttcata caaatcaaca
     2461 catgtacata aacatgtaaa tcatgcaaag gattctatta ataaacactt tattgttaaa
     2521 ccatcagaat cgcctagata tacacatcca tctcaatctt taattatcaa gcatcatttt
     2581 gcagttcctg gatatcacgc gcataaattt gttacaccag ggcatgctag cattaaaatt
     2641 aatcactttt gtgttgtgcc acaaataaat agtttcaagg taattccacc atatggtcac
     2701 aattcacatc gtatgcatgt accaagtttc caaaataaca caacagcaac acatcaaaat
     2761 gctaaagtaa ataaagcata tgactataaa tacttctatt cttataaagt agttaaaggt
     2821 gtgaagaaat atttctcatt ttcacaatca aatggttata aaattgggaa accatcatta
     2881 aatatcaaaa atgtaaatta tcaatatgct gttccaagtt atagccctac acactacgtt
     2941 cctgaattta agggtagctt accagcacca cgagtataaa aattggcact aagtttacga
     3001 gatatgataa atacctatta ttttaaatat agtctacaat ctatgtggtt gtaggctgta
     3061 ttttttgcag tttatcaata aacacccatc aacaaattat accgtttttc tactttgaaa
     3121 gttggaagta acataatctt aaataaatat attattaatt aagataaata taatactcaa
     3181 gattattgtt aatagtttgt tcatcgcaag ttaattattg tttctaaaat attggtatat
     3241 aattttcaat ggcgaagaaa acagggtgaa aaagtcggtt tttatatcaa agcaaataag
     3301 ggagcataaa caaatgaaaa ggaaagtatt agtactaaca atgggtgtaa tttgtgcaac
     3361 tcaattatgg cattctaatc acgcaaacgc attagtaaca gagagtggcg caaatgatac
     3421 taagcaattt actgaaattg tatcggaaga gaaagttata acagttgaac atgctcaaat
     3481 taatattttt caatctaata gcaattcaaa cttgatggag ttcaacatat taacaatggg
     3541 cggtaaatca ggagctatgg ttggttatag tgaaattgac tcatcacatt tcacagaccg
     3601 tgacaaacgc gttattagac gtgatcatgt taaagaagca caaagcttag tagagaacta
     3661 taaagataca caaagtgctg atgctaggat gaaagccaaa caaaaagtta acacattaag
     3721 caaaccgcat caaaactatt tcaataaaca aattgataag gtttataatg gattacaacg
     3781 ctaatccaaa gtaaattata agttatacat ctcgttttta aatgacaatt tatccccgta
     3841 aatattataa ataatctttt caaattccac atagatatag agacactaat aaacctcttt
     3901 gtctcgatat gatagtctgc aacgattcat gttgtaggct ttttaatttt acaaataagg
//
LOCUS       DAKTIP010000206         3912 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAKTIP010000206 DAKTIP010000000
VERSION     DAKTIP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.209"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..176)
                     /locus_tag="O0305_002425"
     CDS             complement(<1..176)
                     /locus_tag="O0305_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCY0275534.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSL"
     gene            complement(176..1945)
                     /gene="ilvB"
                     /locus_tag="O0305_002426"
     CDS             complement(176..1945)
                     /gene="ilvB"
                     /locus_tag="O0305_002426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCY0275535.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            complement(1973..3661)
                     /gene="ilvD"
                     /locus_tag="O0305_002427"
     CDS             complement(1973..3661)
                     /gene="ilvD"
                     /locus_tag="O0305_002427"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCY0275536.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLTLPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
BASE COUNT         1169 a          769 c          690 g         1284 t
ORIGIN      
        1 tgaagtgatg tatcatcttg aatatcgact tgaatttcca tgttagaaat cccaggttgt
       61 tcagaatgtg taacatgtaa tgtatcgata ttatattgta ggcgaacaaa agcacttgta
      121 attcgattta gcgtgctgac ttgatccgca acttgtaatt taagaattct tgtcattata
      181 agccctccat ttcatgattt gatttgccac tcggaaccat tggggttaca gcttcagtag
      241 gggaaatacg aacctcaatt aaagctggtc cttgataagc aaacgctgca tctaattgtt
      301 cttccagttg ttctggctta tcgattaaga aacctttgac gccatatgct tctgccattt
      361 tcataaaatc aggttgacca ttaaatactg agtgtgagaa gcgttgatta aagaacttat
      421 cttgccattg tttaaccata cctaatgttc cattattgat tagtacgatt ttgacatcta
      481 aaccatattc gggtaaaagt gccatttctt ggtttgtcat ttggaaacca ccgtcaccga
      541 cgaaacatac gactgtttta tcaggattag ctaatttggc accaattgac gaaggaatac
      601 cgaatcccat tgttcctaaa ccaccgcttg taacccattg tccgtgattt ttaaatggat
      661 aaaattgagc tgcccacatt tgatgttgtc ccacgtctgt agtaacaatt gcttcaccat
      721 ttgtaatttt gccgatatat tcgattgttt gttgtggctt acaaaatact tgatcttctt
      781 caccaagttt aaatgggtgt ttctgcttat tattttgaca atgtttaacc cagtcactgt
      841 gttctatagt ctcaacattt ttatcattta aacattctaa aaatctttta cagtctgcaa
      901 taatacctaa atctacatga ataactttat tgatttctga aggatcaata tctacatgta
      961 caattttggc gttaggtgca aaggcatcag gtttgcttgc taatctatca tcgaagcggc
     1021 tacctaaatt aatgagtaaa tcacactcag ttaatgccat gttactagca taagaaccat
     1081 gcattcctcc catacctaaa aatagtgtat cctcataagg tacagcacct agacctagta
     1141 aagtagtaac tgttgggatt tgatgtttat taacaaactg tgttaataat tgatttgatt
     1201 ttgattgatt aatacctgcg ccggctaata cgacaggctt tttcgcttct tttaaaagtg
     1261 agatgaaagt gtcaatgtct ttattttctg gttctgtaac aacttcataa cctggaatat
     1321 tgatttcgtc gcataaatcc acatttgtag ctaaaacacc catatctttt ggaaaatcaa
     1381 tcactacagg acctttgcgt ccagaattag ctacatggaa agcttcgtgt acgattttag
     1441 ggatatcttc aacacgtttc acttgataat tttgttttgt aattggtgaa gtcatagata
     1501 gaatatccgc ttcttggaat gcatctttac caatgcctgg tgtagcaact tgtccagtga
     1561 atacaactag aggtaaagag tcgcaatgtg catccgtaat acctgtcatt acattagttg
     1621 cacctggacc gcttgtaact acaacgacgc cagttttacc agatacacgt gcataacctt
     1681 ctgcagcatg aacagcacct tgttcgtgtc ttgctaaaat atgtttgatt ttaccatcat
     1741 aaaacgtgtc atataaaggt agtacggcac caccaggata accgaataaa taatccacat
     1801 tttctttaag tagagcttct actagcactt ctgatcctga acgcatatca tttaaagttt
     1861 tttcatttag ttgttcaggt tcaagtgatt cagccatttt taaagggtca atattttggt
     1921 ttacttcatg ttgagtttta gacattttaa ccatctccaa tataaaaata aattaaatta
     1981 aattctcagg gacttgcatg acgccacctg tatttgcgct agttactagg gcagtataac
     2041 gagctagata acctgttttt actttcgctt taaatggtgt taaagattct cggcgacgcg
     2101 ctagaacatc ttcaggctgg tttacgttta atgtacgatt tgttaaatca atagtaatct
     2161 catcaccatc ttcaattaag gcaattggtc caccagatgc agcttcaggg gaaatatgac
     2221 caactgcaat acctcttgtg gcaccggaaa aacgcccatc agtaattaat gcaacatctt
     2281 tacctaagcc gcgaccaaca atagaggaag taggtgctaa catttcaggc atacctggtc
     2341 cacctttagg tccttcatat ctaatgacaa cgacgtggcc tgcacgaacg gtacgattgt
     2401 ctattgcttc aacagcttca tcatgcgaat tgaaacaaat tgctttccca gtaaatgttt
     2461 tgatagatgg atcaacgccg ccaactttaa taactgcgcc tttaggggcg atattaccaa
     2521 ataagataga taaaccgcct tgtgcatcat atggtgcatc aagagggtga atgacatcaa
     2581 agttcttaat ttctttgcct tcgttatttt cacgtaacgt tttgccagta actgtgattc
     2641 tatctgggtg taacgtgcca tctttcttca tcaattcatt aataattgct gggacgccac
     2701 cagcttcatg cacatcatgc attgaatacg atgaactagg tgctattttt gataaatatg
     2761 gcgtgcgttt ggcaatagca ttaatgcgct ctaagtcata atcaatacca gcttcattgg
     2821 caatggctaa cgtatgcagt accgtgtttg ttgaaccacc catagccata tcaagtgcaa
     2881 atgcatcatc aattgcttcg cgagtaacga tatcacgtgg ttttaaatca tttttaatat
     2941 tttcaactaa tttaaaagct gcttggcgaa tcatttctcg tcgctgatca ctgactgcaa
     3001 gtgcagtacc gttgtatggt aatgttagac ctaaaacttc catcaaacag ttcattgaat
     3061 ttgcagtaaa catcccagca catgaaccac aagtagggca ggcattttgt tccatatcta
     3121 aaaattcttc tttagaaatc gatccttctt taaatgcgcc gactgcttca aacattgatg
     3181 aaagtgttaa tgcttttcca tgtgcagata agccagcttt cattggtcca ccagagcaaa
     3241 agatagctgg tacgtttgtc ctcatggctg ctaaaatcat accgggtgta atcttgtcac
     3301 aattaggaat gtaaaatacg ccgtcaaacc aatgagcgtt aattacagtt tcagctgcat
     3361 ctgcaataat ttcacgtgat ggtagagaat atcgcattcc gatatgtccc atagctattc
     3421 catcatcaac accaattgta ttgaattcaa atggaatggc accggcttct ctaattgctt
     3481 ctttagctat atctgcaagc tctctcaagt gaacatgtcc aggaacaata tcaatataag
     3541 agttacaaat agctacaaat ggtttgttca tatcagttgg actttttagc gcgcccgtgg
     3601 catgtaaaag acttcttgct ggtgcttggt gatctccttt tttgatcatg tcgcttcgca
     3661 tagtaaattc ccccgtaaat tttaatgatt aatcatgttt tatagtaata aaaataagta
     3721 attgaaagat aaaacataaa aaaacgtccc aatattattg ggacgtttac aagtcccatt
     3781 caaacagaaa taggacttaa agcgtttagt aaatatttac taacgttcga gtcctaaaga
     3841 gtataataaa ataataatat ttgaagttgt cggttgatgt tcagtatatt gattaagcat
     3901 tttattatac tc
//
LOCUS       DAKTIP010000207         3863 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAKTIP010000207 DAKTIP010000000
VERSION     DAKTIP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.210"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(134..319)
                     /locus_tag="O0305_002428"
     CDS             complement(134..319)
                     /locus_tag="O0305_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275537.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            complement(408..1472)
                     /locus_tag="O0305_002429"
     CDS             complement(408..1472)
                     /locus_tag="O0305_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCY0275538.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0305_002430"
     CDS             complement(1620..3062)
                     /gene="lmrS"
                     /locus_tag="O0305_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCY0275539.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTATGLI
                     IDQASWRVPFIIILGIAILAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALILSMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O0305_002431"
     CDS             complement(3384..3857)
                     /gene="sepA"
                     /locus_tag="O0305_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCY0275540.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
BASE COUNT         1333 a          742 c          499 g         1289 t
ORIGIN      
        1 atagttacgt ttttggaatt aacgttgaca tattgtcatt cagttttcaa tgttcattaa
       61 tgttcaatct cttttattct acttcattta tttcttgaag tcaataactt tttgaacgat
      121 tactttattt attctatatt gtttttatag ttatttcaat ggtaagtttt acacttttga
      181 aattcttctt taaaaacaac tgcgtcgttt ttgacgactt tatcatatta tcaactttgg
      241 aatttaaagt caataacttt tttaaaaact ttttgtgttc acaacccgct tctttttcaa
      301 cgcgtttatt gcttaacaca agaacttatt ttaccagcat tccaaaacaa atcaacataa
      361 aaacgtacaa aataaaagta attttgtacg tttagcatat attataccta tttatttgta
      421 gcagctataa ctttttgtgc aatcgagcta taaattttac ctagacgatc atctgattga
      481 tatattgacg gtgcaaaatc ttttggattc caagatggtt gctctaaagg taattcccca
      541 agtaattgag tattaagttc atcagctaac ttagtaccgc cacctttgcc aaagacatat
      601 tctttattac ccgtctcttt actttcaaaa taactcatgt tttcaattac gccaagaata
      661 gaatgatccg tatgttttgc catcgcacct gcgcgagctg caacaaatgc tgctgtagga
      721 tgaggtgtcg ttacaataat ttccttactt gaaggtaaca tcgtatgaac atctaaagct
      781 acatctcctg ttccaggtgg aagatcgagt attaaatatt caatgtctcc ccatttaact
      841 tctgtaaaga aattcgtcaa cattttacct aacattggcc ctctccatat aactggcgca
      901 ttttcttcca caaaaaaggc cattgatata actttaacgc catgacgttc aactggaatt
      961 acttccttcc ctttaattcc aggcttttca tcaataccca tcatatctgg tacactaaat
     1021 ccatatatat cggcatctac taatccgact tttttccctt cacgagctaa ggcaacggct
     1081 aaatttactg caacagtaga tttaccgaca ccacctttac cggaggctat tgcaataaat
     1141 tcgactggat tattttgaga taataggcct tctatcgttt taggtttatt ttcttctttt
     1201 ggtttaaatt gatttacttt ttcttccggc aatgtttcaa atcgtatacc gaccgttttc
     1261 gcaccgtttt cttttaatgc attaacaaca gccatctgta aatctaattg cggtgcacca
     1321 cctaattgtg ccattgctaa tttaacactc acatgttctt tttcttcctt aatagaaact
     1381 tcaacaatac cttctgtttc ttttaaaggc acatctataa taggatcttt aatttctcct
     1441 accaattctt tcacttgatc taccgttaac aaagcaaatc ccccttaatc attttaagac
     1501 atctacatca tagcaaataa caatttttgc ttagtctatc actaaaagta taaaactttt
     1561 tattaaataa aaaaagcagt tagcctatta ttttaaatag accaactact ataattattt
     1621 taaaatttcc ttctattact ttctatttct ttaggttctc ttataaaaag tgctatcaca
     1681 taagctgcca taacaagcac agctacaaaa gcaaatgaga tatccacccc tatagcaatg
     1741 gattgctgtt taatttcagt tgcagttaaa tgcgatggcg atgtagataa aaatattttt
     1801 gcaccaaaac tcattaatgt aaccattaaa gctgtgccta gtgaaccagc cattacacga
     1861 ccaaagttca taatagccgt gccatgtgag atttcttcat ttctcaaaga attaattcct
     1921 gtagtattta tcggcatcat gagtaaagaa actgaaaaca ttctaattgc atatacgact
     1981 attaaatacg tataagatgt gtctgtatgc aagaaacaca acataattgt agtaattgtt
     2041 aaaattgtaa aaccagtata aatcaatggt ctaggaccat acttatcata aaatttacct
     2101 gtaaaaactg acataatacc atttattatt gcaccaggca tgataacaag tcctgataac
     2161 aaggcagata aagataaact gttttgaaca tatagcggta taagcagcgc aggtccaacc
     2221 attgacatca ttataatcat tgaactaatc gtacataatg caaatgttct atttttaaaa
     2281 acccttaaat ttaaaagtgc attagtaata ttaaattgtc tacgtataaa taaatagata
     2341 ataaccatac ttaatatcaa cgcacctatt actattggac ttgtaaatcc taaatctcct
     2401 gcactactaa atgcgtatag cattaacccg aacccaatag ttgaatacat tactgaacgc
     2461 ttatctaatt tcgtatattt cacttcattg taactcgaga ttgaaaccaa accgaaaaca
     2521 aaggcaagta tagcaattcc taaaattata ataaatggaa ctcgccaact cgcttgatca
     2581 ataattaatc ctgtagctgt aggtcctatt gctggtgcaa attgaattac taaacctgct
     2641 agtcccattg caaaacctcg atgttcttta ctgaacaatg taaataacgt aaattgcatt
     2701 aagggcataa gtacgcctgc acccatcgct tgaattacac gagctaacat taaaactcca
     2761 aaattcggag ctaaggctgc aacaatagaa cctaataaaa atgtccccat cgccgctaag
     2821 tataaaggtc tagttttaat tctatccatt agatatgccg tcagaggtat catgacgcca
     2881 ttaacaagca taaacccagt aactagccat tgcgatgtac tttcattgat atttaattct
     2941 ctcattatac taggtaacgc tgtatttaat aatgtttgat ttaaaatagc tacaaacgca
     3001 ctaatcaaca taacagcaac tatgaagttt cgtctccggg ttgttaattc aactttagcc
     3061 atcataattc ctccttttta aaacagatat acacttgtcc ctttaatacg tatagccatg
     3121 aacataatgg gagtagtact caaataataa agaagcacta cataataaat cattattggt
     3181 tcttacacat taggcacagc taatgtgtac ttaaaaacta acaatataac attgtagggc
     3241 ctaataattt attttatgtc tcagactcat acaatggtta tttaacttat ttaccacaaa
     3301 ttgagcacta agactcacta ttatcgattc ttctattgaa caaaaagaag ttggaacaca
     3361 tatgtgccct caacttcaga tatctatttt ctattattta aatttttact ttcagggtaa
     3421 aattgaagct tacaaacata taagaaccct ataataacca tgatgaccca aaaatcgata
     3481 ttgatataca catagttcag atgaataata aaataaaata acgttgttgc aactgtgtaa
     3541 gaaatcaaat gaaaagtcgc gcctctaaaa tatgcatttt caggcaacat ttttctaaaa
     3601 taatccatta aatgctcgac tgcaaatatg atgaagtacc caaagattat ataactgcca
     3661 taatataata ggttatcgta catacttcta ttatatgtaa agtcacccaa cctaaaataa
     3721 ataagtattc tacttaatcc atataaacca aaccctagaa atgttaaaaa aatcgcacca
     3781 gaaagtacaa aaataaagag aacaatcatt gtagttaaca agttataaaa cttatgttta
     3841 agataattca caatcattgg cct
//
LOCUS       DAKTIP010000208         3851 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAKTIP010000208 DAKTIP010000000
VERSION     DAKTIP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.211"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..400
                     /locus_tag="O0305_002432"
     CDS             <1..400
                     /locus_tag="O0305_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCY0275541.1"
                     /translation="FYMVTDLIHLNQFQFNFYAGLNLSEWKYFTYLLLGVFLAVYVMI
                     LIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVYFFNEPLHVLNHILES
                     LTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            397..681
                     /locus_tag="O0305_002433"
     CDS             397..681
                     /locus_tag="O0305_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275542.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(836..2209)
                     /locus_tag="O0305_002434"
     CDS             complement(836..2209)
                     /locus_tag="O0305_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCY0275543.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYNVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEADFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(2295..3848)
                     /locus_tag="O0305_002435"
     CDS             complement(2295..3848)
                     /locus_tag="O0305_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCY0275544.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         1277 a          711 c          579 g         1284 t
ORIGIN      
        1 attttatatg gtaactgatt taatccattt gaaccaattt caattcaact tttacgcagg
       61 actaaattta agtgagtgga aatactttac ctatttactg cttggtgtct tcttagcggt
      121 ttatgtgatg attttaatct attttgaaat actaacattg acttctcacc gttggcttaa
      181 tagattaatt gttgtcgtgc taatgttttt aaatggattc ggaatttata ttggtcgttt
      241 cttacgctta cattccgttt atttctttaa tgagccctta catgttctaa atcacattct
      301 agaaagtcta acgcttaaaa ctgctatgtt tgtatgtttt atggttgtca tgcaagcagc
      361 aattctatta tttggaaaag gagtgcgttt gaacaagtga caataaatat aatcgtttta
      421 atagtatcta tcattgtctt tcaattgatt attggacaca tatggcatga tattggttta
      481 agttatttaa gaagtatctt actcatgatg ctgccatttg gactaggtgt atttatacaa
      541 caagtgtcgt attacgaacg acaatatcct aaatggcaag tgcctcaaaa cataaaagtt
      601 agattgaaat atatttatct cgctacgttt ttagagtatg tcgtactata cctcacttta
      661 tttacggaca tactaagata acatcaaatt aataagaaaa ctaaatacaa cttatatatt
      721 tgaggaaagc caatttctat cacatcaata atagaaattg gcttatttat attgcttaag
      781 aactttatat tctgctccag attttactca tgccagatct atcaattgag ccgaattagt
      841 ctagcgcttt aaatgtagct aaaacttgtg tacgtttttc ttgatgatct accatttgtc
      901 ttggataatg actacctaat tcaatcccct gttcaaaaag gttggactca ttgcgttgtg
      961 tatcatgcaa atattttgca tcaatttgat taaaaatcgg aagatatgtt ttgatataca
     1021 aagctttagc atcaaagcgt tcactctgtc ttattggatt aaacattcta aaatacggca
     1081 ctgcatccgt acctgtagaa gcagaccatt gccatccatg aatatttgat gctgcatcat
     1141 agtcaataag gtgctttcta aagaattttt ctccccatgt ccaatctata aataaatctt
     1201 tggttaaaaa ttgcgacaca accattctca ttcgattatg cataaaacca gtttgtgtca
     1261 attccattat tgctgcatca atgattggaa atcctgtttg cccttcgcac catgcattaa
     1321 aatccgcttc attttgcgac cattttatct gtcgatattt aggtttgaaa gcttggtatg
     1381 aggtttcagg atactgtgtc attaacacat aataaaattc tctaaaaatg agttcacgta
     1441 taaatgcttc atagtttgcc tcatcttcat cataacgcgc caataaatca ttaaaaattt
     1501 caataatatc taataatcca tatgctaaag caacacttag ctgacttgtt aatacttcag
     1561 gtaaatagtc tcttccgctt tcgtaattct gtatatcttg atctaaaaaa tgttgccatt
     1621 tattctgttc aattatttgt gaggatccaa atgctatgtc atcaaacact aaatcatcag
     1681 gtgatgcaat gacaaagtct tcgaatgatt ttaaatcata gtgatatacg tctctatgcc
     1741 tcaagtaagg tcgccatttt ttatagaaac tagtaaaaac attgtatggc tccccttgtt
     1801 gattcattgt tttactcgct ttaaagtaat gattcccacg tatcaaagta acggcaatgc
     1861 gtgcttcatt gaataaagaa cgttgatgta aaatatcata ttcttcaaga tgataactca
     1921 taatatcacc cgcagtcact acttcagaga tattgttttc tttacaaaat gaaatgatat
     1981 cgtcataaga cataacataa ggtgtaagct tttctgcgtg caacgattgg taaaagcgct
     2041 gcaaagtacc gcgataaaac tgtgctttca aatcagaagc tgaagacatg tcctcttccg
     2101 gaatgataaa ataacatgca tcaatgtctt ctttttgttg ataaatatat tcaaataatg
     2161 gattgtgttc cattcgaaac attcgattta ataacacagc aattgccata aacaaaacac
     2221 ccttcaatta tctttttatt ttattaagct ttttgtcact ttaaaataac ctagattctt
     2281 atttatattt ttaattaaag caaacctaac catttccaat aagtcatgct aaaaactatc
     2341 actagaatat accctacaat tgtcaatggt atacctgcct tcaagaaatc tttaaccgta
     2401 aaagtaccag tgccataagc caacatattt tgaggtgcac taacaggtaa taagaaacca
     2461 aaactaataa caaattgttg aattaaaaca aatcctatag actggtctcc taagtgtaac
     2521 gtagaggtta gcgaaataaa aacaggtatt aacgctgatg ataaacttgt tgcactcgca
     2581 aagcccaaat gaatcaatat attaaaaagc gtgataagcg caattgtcgc gataataggt
     2641 aaatgtttta aacctaaaac accaaaagtt tgatcactta accattgagc tgcacctgtt
     2701 ttcaaaagaa cgttacctag tgaaatacct acaccaaaca cgataattgt tccccatggt
     2761 attttatttt caacatgttt ccatgtcatg acaccaattt tcggcattaa cataacacct
     2821 aaagcaataa tagtaatgga tgcagagtca atcggatgta atactttttc agttgaccaa
     2881 aacagtaata ataacatcga tataacaatt aaacgccatt cacgtgggct aacggggcca
     2941 agtttatgca attcttcttt tattaaatct ttaccacctt ctattgtatt aatttctgga
     3001 ggcatcactt taatcatgat gaaatataaa gctacggaca taactatgga ccaaggcgct
     3061 gcatataaga accactcgcc ccatgaaaca tcaaatccta attgatggtt tataaaatta
     3121 atcgctacga tattttgtgc tgccgccgtt ttgataccaa tattccaaat tgacacagct
     3181 tgtactgaag taattattaa taaagacgct aacttgctat ctttggaaac tttaaatgcc
     3241 gcaatcatac ccagcaagat tggtacaact gcccctgctc tagctgttgc agaaggaacg
     3301 aaaaatgcaa gtacaattga aacgataatt gctccaataa ctatatttct agttttatta
     3361 ccaacaattg ataacactaa aagagctagt cttttatgca aattcgtttc ttgcatagca
     3421 gcagccaaaa ataatgcagc tgcaacgaga gctacagctg aagttgcaaa tccactaaac
     3481 gctaatgata atgcatgatt agttcctaga aggtcacttc cagctaaaat agcactgcca
     3541 cttttcggat tacctagctt ctcccctaaa ttttgaacag ggctaaatcc taaaagtaat
     3601 atcattaagc caataattaa agttgctgac accggatatg atacagcttc cgttacccac
     3661 ataataactg caaaagctaa aatagctagt actgccttag ccattatcgg cagacttgaa
     3721 ggtgccggga gtaatagtac aactataagt attataaaac tcatgacaat ccaaaatggc
     3781 ttgtattgct tttttgtctt ttctttcttt tgttgttcat gttcaactac atgtttttct
     3841 tctgacataa c
//
LOCUS       DAKTIP010000209         3841 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAKTIP010000209 DAKTIP010000000
VERSION     DAKTIP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.212"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..154)
                     /locus_tag="O0305_002436"
     CDS             complement(<1..154)
                     /locus_tag="O0305_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275545.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIAT"
     gene            328..1125
                     /locus_tag="O0305_002437"
     CDS             328..1125
                     /locus_tag="O0305_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCY0275546.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1265..3025
                     /locus_tag="O0305_002438"
     CDS             1265..3025
                     /locus_tag="O0305_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCY0275547.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSDGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            3026..>3841
                     /locus_tag="O0305_002439"
     CDS             3026..>3841
                     /locus_tag="O0305_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCY0275548.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRD"
BASE COUNT         1379 a          562 c          740 g         1160 t
ORIGIN      
        1 ttgttgcaat tgttgaatat ctctctgcta aacgatatgc attaatgtaa agctttaaac
       61 tttctttagc tatatcctct gcatcttcga attttgatgg gttagacata accactaatt
      121 ctgcaaattt ttctggatca atattaatag acatgtattt atttacaact cctatttatt
      181 ttgatgtctt aatactaaca tattgaagtt ttcagacaaa gtaatgtctc tctataattg
      241 aagaaaaata attcaaagaa tttaatattt actttaaata ttagaacgta tgaattaaac
      301 tagtatttat gagaggatga acaaaacatg agaataatgt ttatagggga tatcgtaggt
      361 aaaattggac gagacgcaat tgaaacgtac atacctcaac tgaagcaaaa gtataaacca
      421 acagttacaa ttgtaaatgc tgaaaatgca gcacatggta aaggtttgac tgaaaaaata
      481 tataaacaat tactaagaaa tggtgtagat ttcatgacta tgggtaatca cacatatggt
      541 caacgtgaaa tttatgattt tatagatgaa gcaaaacgac tagtaagacc agcgaatttt
      601 ccggatgaag cgccgggaat tggtatgaga tttatacaaa ttaatgatat taaacttgca
      661 gttattaatc tgcaaggaag agcgtttatg ccagatattg atgatccttt taaaaaggca
      721 gatcaattag tcaaggaagc acaagaacaa actccgttta tatttgttga ttttcatgca
      781 gaaacaactt ctgaaaagta tgcaatggga tggcatttag atggtagagc tagcgctgtt
      841 gttggaacgc atacacacat tcaaacagca gatgaacgta ttttaccaaa ggggacaggg
      901 tatataacgg atgttggtat gacaggtttt tatgatggca ttttaggaat aaataaaaca
      961 gaggtaattg agcgttttat cactagtttg ccacaaagac atgttgttcc aaatgaaggt
     1021 agaagtgtat tatctggtgt tgttattgat ttagacaaag aaggtaaaac aaagcacatc
     1081 gaacgtatat tgataaatga tgaccatcca ttttcaacat tttaaaatta cgtaagtaaa
     1141 cattcgaatt ggaccctatc gtccattagt atgaatttaa tatagtacca ctgtttacat
     1201 agtaaatcgg tggttctttt tgttatcatt taatatgaaa tatatccata ggaggcatat
     1261 aactatgaaa ccacaattat cgtggaaagt tggcggtcaa caaggcgaag gtattgaatc
     1321 aactggggaa atcttcgcta cggctatgaa tagaaaagga tattatttat atggatatag
     1381 acatttttca agtcgtatca aaggtggaca tacgaataat aaaattagag tttctacgac
     1441 gcctgttcat gcaattagtg atgatttaga tattttgatt gcatttgacc aagaaacaat
     1501 tgatgttaac catcatgaaa tgagagaaga cagtattatt ttagctgatg ccaaggctaa
     1561 acctgtgaaa ccagaaggat gtcatgcaca gcttattgaa ttacctttta cagcaaccgc
     1621 taaagaatta ggtacagcat taatgaaaaa catggttgca ataggtgcta ctagcgcatt
     1681 gatgaatttg aatacaaata catttgaaga acttattact aatatgtttt ctaaaaaagg
     1741 tgacaaggta gttgaagtca atatccaagc attaaacgaa ggttatcaat taatgcaatc
     1801 tcgcttacct gaaatctacg gggactttga attagagtca acagatgcac taccacatct
     1861 atatatgatt ggtaacgatg ccattggatt aggtgcaatt gctgcaggtt cacaatttat
     1921 ggcggcatat cctattacac ctgcgtctga agttatggaa tatatgattg ccaatatatc
     1981 taaagtaaac ggagcggtta ttcaaacaga agatgaaatt gctgctgtaa ctatggctat
     2041 tggtgcaaat tatggtggag tcagagcgtt tacggctagt gctggtccag gtttatcttt
     2101 aatgatggaa gcaattggat tatctggtat gactgaaacg ccattagtca ttattaatac
     2161 ccaacgaggt ggaccttcta ctggattacc tacgaaacaa gaacagtcag atttaatgca
     2221 aatgatttat ggtacacatg gtgatattcc aaaaattgtt gtagcaccaa cagatgcaga
     2281 agatgcattt tatttaacta tggaagcatt taatttagca gaacaatatc aatgccctgt
     2341 tatagttcta agtgatttgc aattatcttt aggtaaacaa actgttgaaa aattagatta
     2401 taatcgtatt gaaattaaac gtggtgaaat cattcaatct gatattgaac gtgaagaaga
     2461 tgataaaggt tatttcaagc gttatgcgtt aacatccgat ggtgtttctc ctagacctat
     2521 ccccggtgtt aaaggaggta ttcatcatat aactggtgtg gaacacaatg aagaaggtaa
     2581 acctagtgaa tctgcgtcaa atagacaaca acaaatggaa aaacgaatgc gtaaaattga
     2641 gcagttacta attgaatcgc cagtagaagc taacttacaa catgaggatg cagatattct
     2701 ttatatcggt tttatttcta caaaaggtgc aattcaagaa ggtagtaacc gtttgaatca
     2761 acaaggcata aaagttaaca ctatacaaat tagacaattg catccattcc caacaagcgt
     2821 tattcaagat gcagttaata aagcgaagaa agtcgttgta gtggagcaca attatcaagg
     2881 acaattggct agtattataa aaatgaatgt caatattcat gataagattg aaaattatac
     2941 aaagtatgat gggacacctt tcctaccaca tgaaatcgaa gaaaaaggca aaataattgc
     3001 tactgaaata aaggagatgg tatagatggc gacatttaaa gattttagaa ataatgttaa
     3061 gcctaactgg tgccccggat gtggcgattt ctcagtacaa gctgcaattc aaaaagcagc
     3121 cgcaaatata gggttagaac ctgaagaagt agctatcatc accggtatag gatgttctgg
     3181 ccgtctttca ggatatatta attcttatgg cgttcattct attcacggac gtgcattacc
     3241 tttagctcaa ggtgtaaaaa tggcgaataa agatttaact gttattgcat cgggaggaga
     3301 tggtgatggt tatgctatag gtatggggca tacaatccat gctttaagaa gaaatatgaa
     3361 catgacgtat atagtcatgg ataatcaaat ttatggtttg acaaagggac aaacatcgcc
     3421 gtcatcagca gtaggatttg ttactaaaac aacgccaaaa ggtaatatag aaaaaaatgt
     3481 tgcgccttta gaattagcat tatcatctgg tgccacattt gtagcccaag gtttttcaag
     3541 cgatattaaa ggattaacaa aactaattga agatgcaatt aatcatgatg gattttcatt
     3601 cgttaatgtc ttttcaccat gtgtgactta taataaaatt aacacatacg attggtttaa
     3661 agaacattta acaagtgttg atgacattga aaattatgat tctacagata aacaattagc
     3721 gactaaaact gttattgaac atgaatcttt agtaactggt attgtttatc aagataaaga
     3781 aacaccatca tatgaatctc aaattaaaga gttagatgat acaccacttg ctaaaagaga
     3841 t
//
LOCUS       DAKTIP010000210         3835 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAKTIP010000210 DAKTIP010000000
VERSION     DAKTIP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.213"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            49..3201
                     /locus_tag="O0305_002440"
     CDS             49..3201
                     /locus_tag="O0305_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCY0275549.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQHIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIKEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            3258..3740
                     /locus_tag="O0305_002441"
     CDS             3258..3740
                     /locus_tag="O0305_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275550.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         1502 a          485 c          712 g         1136 t
ORIGIN      
        1 ttaaaatagc attaagtaaa aaacaaatag ggaacaaggt gggatttcat gagtcaattg
       61 ctaaatgata cgttatcggc ttggttgtta attgaatctt taagtccagg agaagtaaat
      121 tttacagcgg aagatatact ctcagctgaa cattttaaaa atggtgcaaa gcaagcacaa
      181 cttcaaagtt ttgatgaata ttttgaaata tggaatgctg aacgctttat tatatcagaa
      241 gaaaaatcag agactgggga acttatattt aaattttata gacattgctt ccgctataat
      301 gaaattaatt tgaaaattca ggatattttt gatgattatt ctgagattca taatccaaat
      361 gggacacact gttatggtta cacatttaac acagataaac acggcaaagt gatagttgat
      421 tctatacata ttccgatgat tatgagtgca ttaaaagaaa ttgaaaagaa caaaaatgcc
      481 aatatagaag aaaaatttaa tgattctgtt gaaaaatttg ttcaaaaagt aaaagaaatt
      541 ttagcagatg aaccaattaa tgaatttaaa ttgaagaaga tggacaaagc ttatgatgag
      601 tacttttctg tattaaattc aaagaaagat ggattatttg gacattatgt agcaatagaa
      661 tatgtgaaag atagtgattt accacagccg gaatttaaca gcttcttcat aagtgatatt
      721 gagaaagcaa gaaaatctcc caaccaaact ttaattgatt acattgaagg tgtagaagaa
      781 agtcagcaca tagaagtaga tgaaaataaa gaaatgtttg acaaattttt acatccttca
      841 cgtttgcctg atggacgatg gccatcacag actgagttta gattgtcttt aatgcaacaa
      901 cttgctgtaa accaaattac gagtggtaat gaaagaataa gttcagttaa tgggccacca
      961 ggtacaggta agactacttt attaaaagat atatttgctc atctagtagt tgaaagaggt
     1021 aaagagttag ctaaactaaa taatcctaaa gatgcatttg tcaaaacaaa aattcatgaa
     1081 acggatgata aatacgtata cttactaaag gaatctattg ccaaatataa gatggtagtc
     1141 gcatctagta ataatggagc tgttgaaaat atatctaaag atttaccgaa aattaaagaa
     1201 attataagaa atcccgaaaa atgtaaattc cctaaatatg aacagaatta tgcaaattta
     1261 gcacatgaat taaaagattt tgctgaaata gctgaagatt tgattggtga aagtgcctgg
     1321 ggcttatttt ctggagtttt tggtaaaagt actaatatta accaagtatt gagtcatatg
     1381 ttaaaacaag atgcgaatga tattggcttt gctaaattac tacaaaatga gaataatcgt
     1441 atgagttata acgagttaat gagtgaatgg caatcacatc aacgtgcatt tttagaagag
     1501 ttgaggcatg ttgaaatgtt aaaagaagaa tctattagag catatgatgt ttataaaaat
     1561 tgtgagtctt tctctaagat tgaacaggtt attaatagtg aaaaaacaag tattgaagaa
     1621 caggtatatc atttagataa tgaaacgtta cgagacaata aagaaataga agatttggat
     1681 aatcgaatta attatattgt taagcaaata gaaactttaa atgagttaat taaatccatc
     1741 aaagaaagca acaaaggttt tattaacaaa ctgaaagcga tgtttaattc agaagaagat
     1801 gaaagctata aagatcataa taaagagaag caacaattat taacacaaca gttagagtta
     1861 gagaaatgta aaaaaaacaa acatgaagac cttgttagca aactaaaaga aaaagagaaa
     1921 ttaattaaac aattaactaa agtacagttg caattagacg agttaaattc acagttacaa
     1981 gagttagaag catatcgtat tgagtcaaaa attacaattc cagaaaaaga tttttggagt
     2041 gacaacaatt atgatgagcg ccaagttact aatctgtgga cgagtgacga acttcaatac
     2101 agacgtgcca tgctcttttt aagagcaatg atattgcata aattattatt gattgctaat
     2161 aatacaacta tttattatgc gattaatgat tttaaagata gaaggaaatt aattgatgca
     2221 aatccagata aagtacacaa cgcatggaat gtgatgcatt taatatttcc agtagttagt
     2281 acgacgtttg caagctttaa atctatgtat gggggcatac caaaagattt catagactac
     2341 ttatttattg atgaagcagg acaagcaata cctcaagcag ctgtgggagc attatatcgt
     2401 tcaaaaaaag ttgtagctgt aggtgatccg attcaaatag aaccggttgt gactttagaa
     2461 agtcatttaa ttgataacat tcgtaaaaat tatcatgttc cggaatatct agtttctaaa
     2521 gaagcttctg tgcagtctgt tgcagacaac gccaatcaat atggtttttg gaaatctgat
     2581 gctactgata gtaatcaaaa aacctggata ggcatacctt tatgggtgca cagacgatgt
     2641 ttaaaaccta tgttcacgat agctaaccaa atcgcttata ataataaaat ggtgttgcca
     2701 agtaatatta caaaagtagg taaaacaggt tggtatgacg ttaaaggaaa cgcagttcaa
     2761 aaacaatttg tgaaagagca tggtgaaaaa gtagtgggat tattagctga tgattggatt
     2821 gaagcaatta aggaaggtaa aaatgaaccg agctcatttg taatatcgcc tttttcagca
     2881 gtacagcaac agattaaacg tatgttaaag caacaactac cgactagaat tgatattgaa
     2941 cgtacaaaaa ttaatcaatg ggtcgataaa tccattggta ctgttcatac ttttcaaggt
     3001 aaagaggctc agaaggtgta ttttgtaata ggtactgata atacccaaga tggtgctgtg
     3061 aactggtcat gcgaaaaacc aaacttgtta aacgttgcag tgacaagagc taagaaagag
     3121 ttttatgtaa ttggcgacat gcaaagaata cagatgaaac cattttatga gacgattttt
     3181 aaagaaagaa atgtaaaata acatacaaaa aagtatatag aggaagttat acattttaaa
     3241 aggagcaaaa ttgaataatg gagaaattta acaactggat attaaatgca ataagtggat
     3301 ctcaaacaga caagaatgaa acaactgaag aattaaaagg ggcaaaattt atcattttat
     3361 atgcatattc aatgctcgtt ttgcttgcgt tagtaatttc taacatattc attcacattt
     3421 tggagcctaa actatcaatc accactcaaa tcatcatcgt tttgatttta attgaagcac
     3481 taattggact gcgtttcttg aaagcgtacg atgttaagcg tggcaaagat aaagaaaata
     3541 agaaaaatag taaggatttc gttaaactaa aatcaatttt agtagcaatt ttatttacat
     3601 cattggcgct gacagcaggt actgtagctg atatatacgg tttcactgac ttaggaaata
     3661 ctagaagtga tttaatcgtt tggagcatag gtggtattat atttggcctc gtatgttaca
     3721 caatggaaga taaaagataa cgataaggag ctggcgatta taaagctagc tcctttttta
     3781 acttatatat gtaaagaact atcctaaggg tttttaatca tatgtcaata atttc
//
LOCUS       DAKTIP010000211         3786 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAKTIP010000211 DAKTIP010000000
VERSION     DAKTIP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.214"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..699)
                     /locus_tag="O0305_002442"
     CDS             complement(<1..699)
                     /locus_tag="O0305_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275551.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            complement(692..1465)
                     /locus_tag="O0305_002443"
     CDS             complement(692..1465)
                     /locus_tag="O0305_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275552.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENVSRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKGSKEEALSKVDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            complement(1452..2282)
                     /locus_tag="O0305_002444"
     CDS             complement(1452..2282)
                     /locus_tag="O0305_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275553.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITLIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRLLKQRGEYDESH
                     "
     gene            complement(2275..3261)
                     /locus_tag="O0305_002445"
     CDS             complement(2275..3261)
                     /locus_tag="O0305_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275554.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKIKTNTPLISESSDRHA"
     gene            complement(3432..3617)
                     /locus_tag="O0305_002446"
     CDS             complement(3432..3617)
                     /locus_tag="O0305_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275555.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            complement(3565..>3786)
                     /locus_tag="O0305_002447"
     CDS             complement(3565..>3786)
                     /locus_tag="O0305_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275556.1"
                     /translation="LGVLAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNW
                     RKERKARKLEKQIEKYGYEGAELRRKDKK"
BASE COUNT         1332 a          661 c          530 g         1263 t
ORIGIN      
        1 atatgaaaat gcttgcacta attcttttgt ataagggtgt ctatcaacat taaataattc
       61 ctctattgca aaatcatcga ctatcatgcc atccttaaga acgataattc tattaactaa
      121 gcgttgtaac acggataaat catgagaaat aacgataaaa tgatttaagt tcgtaatcgt
      181 ttgcgctttt aatatattga ttacattttg ttcagctata acatctaaat ttgaagttat
      241 ctcatcacat attaaaacgc gaggctgtgc taataacgaa cgcatgacat taaatctttg
      301 taattgtccg ccactcactt cgcttggtaa tttagtcaat aattgcgcgt ttaactcaaa
      361 agtagataaa tgttgtaata ataattgatc ctgagcagta ttatcagtta gacctctgta
      421 ataatataac gcttctttta atgaggtctc aatcgtccaa tcagggttaa agctagttaa
      481 agggtgttgg aaaatcggta acacagcatt gtcacttaag taaatctctc ctttaacagg
      541 tttaaacaag ccaagaacca atgaagcgag cgtactttta ccacagccac tttcgcctaa
      601 aataccaaca ttttctccat caggtatagt aatattgata tcttgtagca ccatctgctt
      661 tttattataa ccaaaagtca catgttttaa ctcaatcata gtaatccctc tttaattgtg
      721 ttctatattt aattagacgt tcagtatacg gatgcaaatg ctcatacttg aaatgattaa
      781 tattacctcg ttcaatgatt tgaccttctt ttaaaacata aatgtactga caatatttca
      841 atacatgact taagttatgt gtgataataa ataatgtttg accatgttct aatacaatat
      901 gctgtaataa atccatcact tgattaccgt tcaaagcatc caatgatgca actggttcgt
      961 ctgcaatgat taatttaggc tccaacatga gaacgcttgc tatgtatacg cgttcaagtt
     1021 ggcccccaga aagttggaaa ctatatttat ttaatatatc tttgctttgt aaattaaccc
     1081 acgacaaagc cttatcaact ttggacaaag cctcttcttt actaccttta taatgcttac
     1141 gataaatcgc agttaactgt ttacctaatt tagtatggtc gttaaaactt tctgcataat
     1201 tttgagaaat atagccaatt gtatgaccat aatattgact caatctacta acattttccc
     1261 catcaaattg gtacgaatca tacgtgcagc ttaaatcaaa tggtaaatat tcaagtaaag
     1321 ctttagcaat caaacttttt ccagcgccgc tctctccaat caaggcatta atctgttgac
     1381 taaaaatttt caaatcaatc cctttaataa gagatttctc actagtattc tttattgtta
     1441 aattttgtat atcaatgaga ctcatcatat tcaccccgtt gtttcagcaa tctatctctt
     1501 agtgcatcac cggttaaatt aaaaattaaa atagttatag caatgactga agcaggtgca
     1561 atcaacataa ttggatgaga cgaaataaaa tcacgacctt gttgcaacat agcgccccac
     1621 tctggtgttg gcggttgtgc acctaaccca ataaatgata gtgaacttat atatagaatg
     1681 attttaccga aatcaacgac catcaaaacg ataatagccg gtataatttt aggtgttaaa
     1741 tgacgtatta atattgttct tgttggtaca tgaaataatt gtgccatttt tatataaggc
     1801 ttattcattt cgctattaac tatacttcta gtcaaccttg tgtaattcat ccattttatt
     1861 aatgtaattg agataactaa attccataaa gatggttgaa aaaaacttgc taaagcaatc
     1921 atgatgataa attctggaat acttagacca acatcaataa accttaacac taatcgttca
     1981 atccaccctt ttttgtatcc ggcaaataga cctagtgtaa cacctatgac aacgatagct
     2041 attaatgtta aaacagtaac aaacaatgtt gaacgtgcac cgataataat tcgggtaaat
     2101 aaatctctcc cataatcatc agttcctaat aaatgcaacc aactaatagg ttcaaaagtt
     2161 tgtgataaat tgactttggt tgcattttca ctactgacaa agaattgcag tacaattacc
     2221 acaaaaataa atgcaacgaa tacaaaaaat atcaggttat tctttgaaaa tattttatgc
     2281 atgacggtca ctactttctg atatcaatgg tgtattggtt ttgatttttg gatttcctaa
     2341 ttgtaaacgc tgcttcggat caagtaataa cgttaataaa tcagcaatcg tattgataat
     2401 aacaacgaag aagccaataa ataacacgca tccttgaata acaggataat ctcgagattt
     2461 aatactatcc attaatagat aaccaatacc aggtatatca aataaatttt caatcactac
     2521 agtaccacct attagactgc caagtgaaat ccctagtaat gggataatcg gcaaaattgt
     2581 tggttttagt aaatcatgaa ttaaaatata acgttcattc ataccgcgta atcttgatgc
     2641 ttgtacgata ttactttgca ataacatcaa taaattagaa cgcactaaac gaatgatgta
     2701 tgcacacata cctaaagata gcgtgattac aggtaatata aactgactta gtataacgct
     2761 atctatattc attaaatttg tgacaataaa taataaaata ataccgataa agaacgctgg
     2821 taaactaatc gatagtgttg agatcactct aatcacttta tccgtccact tatgaaatcg
     2881 tttggctgct ataatgccga gcggtataga tatgcataac gacactacta atgttgaaaa
     2941 tgatatgagt aatgttatgg gtgcatagtt gaataatatc tgtgttaccg gttcttttga
     3001 ttcaaaactt tttcctaaat taaaatgtaa taaatgattc atccaatgcc accactgtac
     3061 caataaagaa tcatttaatc ccaatttatc tttggttgca tttatttgtt ccgtcgacac
     3121 ttgtgctaca tcaagatgta atattttatc aacaggattg cctggtgata atttcattaa
     3181 aatgaatgta agtgtagaaa taacaaataa aacaactatc atttgcatca gtctatacaa
     3241 catagacttt attatgaaca taatagtccc cctccttgtg taagttacta acactttctt
     3301 tttacatgag aatggcgcat gtatatgcaa cttacatatt aagaactaac gttcattata
     3361 gtattatcca taaagaaatt gaagtatatt taatttttta acaaaatcat tataaaatat
     3421 aatattttga atcaagtcaa ccatgtaaaa tataaaaaag tcaaaacaaa aacaactata
     3481 gcactgtatt ccatctcttt cgaaataatt gttactgcag tgtaacttaa aagtcgatga
     3541 ttttgtgcat atagttgtcg aatattattt tttatcttta cggcgaagtt cagcgccctc
     3601 atagccgtat ttttcaattt gcttttctaa tttacgcgct tttctttctt tacgccaatt
     3661 tctagtaaaa taccataata gaaaactaat taataaactc ataatcgcta aaaatgcagc
     3721 gtatcctaat aatggttgat attttatatc ttgaaaattt ggaataaaaa atgcaagcac
     3781 acctaa
//
LOCUS       DAKTIP010000212         3775 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAKTIP010000212 DAKTIP010000000
VERSION     DAKTIP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.215"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            140..400
                     /locus_tag="O0305_002448"
     CDS             140..400
                     /locus_tag="O0305_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275557.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            429..617
                     /locus_tag="O0305_002449"
     CDS             429..617
                     /locus_tag="O0305_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275558.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            805..1374
                     /locus_tag="O0305_002450"
     CDS             805..1374
                     /locus_tag="O0305_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275559.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1462..1746
                     /locus_tag="O0305_002451"
     CDS             1462..1746
                     /locus_tag="O0305_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275560.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1808..2026
                     /locus_tag="O0305_002452"
     CDS             1808..2026
                     /locus_tag="O0305_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275561.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(2522..2722)
                     /locus_tag="O0305_002453"
     CDS             complement(2522..2722)
                     /locus_tag="O0305_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCY0275562.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(3079..3765)
                     /locus_tag="O0305_002454"
     CDS             complement(3079..3765)
                     /locus_tag="O0305_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCY0275563.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFYFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT         1283 a          598 c          499 g         1395 t
ORIGIN      
        1 atgctcactt gtttgaatta ataaacttta gcattctgca aataatattt taaaaataac
       61 tatacttttt atttacctat tgaaaaatag tgttatcata tttattaaga agctattttt
      121 atactagtag gtgtcagtca tgaataacat tttgttaaat gctatcaata tagttattac
      181 taccactttt gttatcttta atattttaat cacatataat aaagatttag atgatttatg
      241 ttggctcctg cctggtatta tcatttgtgg tgtgatactc atcgtatcct ttaccattgc
      301 aatgataact aaaaactggt taagtgaaat attatttttt ataaatatcg tactcgttct
      361 ctattacatt tatcctattt tttatagttt tataggttaa taattacggt atataaggag
      421 ggtttcacat gtctttacat tttgcaattc tgttttggct agcattaatt ttcttagttg
      481 ccgctacgtt tatactcgta ttaatgaaaa aaactagcaa agaatctaaa aaagagtcct
      541 atttaagttt cactgtcatt ctctatattt ttggattcgc tatattaata tacacattta
      601 tatttggtgt gctataaatg aaattcattt tatatccagt ctgaaacgtc tcaatgatgt
      661 ttcagactgt tttttattct taatagacat ttcacactct catactttaa tatttaagat
      721 atgttttagt cttacaagct tattaaagat tacaattcta atttaagact atttacatat
      781 atttcttcaa caaggagctt ttaagtgaac tatattaaac gaacgataat tttacttata
      841 ttattcgttg tcgtatcccc aataaactca ccaaaaacaa ttgcagataa taagtattca
      901 gaaattcaag atgacaaatt ccaattacaa cctggtgata ttattgttac aaaaggccct
      961 gtcatgtggg gattttttgg tcattgtagt atcgcgattg atgataaaac gattttacaa
     1021 attgaggggc ctggcgacaa accaactaca caatccttcg aatcttttaa atataattat
     1081 gcaagtggca aaaatgattg gatgaaagtt tatcgttgca gctatcctgg tgcaggtaaa
     1141 aaagcggctg aatgggttaa gaaaaactat gagaatacaa atcatcgtta tctagttaca
     1201 ttaaatttga acagcaaaaa atttacgtat tgtactaaaa ttatctacca agcttataaa
     1261 tttggtgtaa gtgaaaaatc agttaaaagt tatggactac atattatttc accctatgca
     1321 attaaagata attttataga tccatacaaa ctaaggcttg ttaaagctta ttaacacagt
     1381 ttcatcaaat agttaaatta gtttgtgttt tgcaataaat tgggtataga ttacaaatga
     1441 tatttcagga ggcccaatat tttgaatgaa ctaactaaag aacaaaagta tacaatagct
     1501 aaattttaca aattatatat tgaacgttcg aataaaggag aaacagaaac agtcgctaac
     1561 ttttttggcg atgctaaaga tgcacgtgaa aactattttt gcgatcgtga ttatcaagac
     1621 ttcttaacta actgccaaat tttaattcaa aacaaatact taactggtga agttttggac
     1681 gataacattt acaatatttc tattttaaac aagacattta ttgaattcga acaaaatttt
     1741 ggttgaacat gaatgtctca actataaact ttctaaccct attttaaagc aggagtgaaa
     1801 aacatacatg agtgaatggc atattaataa tcaatctaca aaaccttttt taatacaaca
     1861 agcggaaaag caattgtcta ttgttaaacc gttgcctaat ggtagcttcc aaatactcac
     1921 agaaataaat ttggagaatg gcaatattag taacatcgat cattctttac ttgtttcagt
     1981 agaccccaaa gcattagaaa taagtatatt tgacgctgta aattaaacgc aatattttta
     2041 tttaattatt ttaatgtttc gccttttaaa taaaaacaag attactgaat tattatgaga
     2101 ttttaaaaac aataaatatt ctcaataatt aatcagtctt aataaaaaat taacaaaaag
     2161 ttgattagtt aaattattaa ttaagtagta agatattatg cgatgtggta tcctattatt
     2221 gttaaacatt ttgagtgttt aacattactt cttgttatcg ccatatacag actttaattc
     2281 tcgtcattat taaagttctg tattggcgct tttttatatt caaaatcaaa agtcggacag
     2341 atgaaaagta aaaaactttt ccatcagttc gatttatttt agaaccatta tatattgtta
     2401 aatctccaac taaatttatt ggacaaaaaa ataacggcta gtgcttttac cacaccaact
     2461 gttatcgatt taaagcataa aaaaacagcc ctatagtaag gactgtttga ataagttaaa
     2521 attacatttt aactacgttt gcagcttgct cgccacggtc gccttcaacg atgtcgaatt
     2581 caactttttg gccttcttct aatgatttgt atccatcttc agcgattgct gagaagtgta
     2641 cgaatacgtc gctaccatct tctctttcga tgaaaccaaa acctttttct gcattaaacc
     2701 atttaactgt accgttattc atattgaata cctccgtcgt gcttttgcac taaatatttt
     2761 gtaacaaatt cataaatgaa aaggagaata ttctatataa ataactcaca tttttcttca
     2821 cgctctttat tacttaactt tcattataca cttttaaata tagaagtaaa gcattatttt
     2881 attttctgtg ataatacaca ttgaactata tagtaacatt ccattttaat actaaattta
     2941 aaatttttat tttctaatat taaatacact tacttttgat actatttact tttttaattc
     3001 tgaattgata ataaatcata tcgtgatcta aaaattataa aagtgccact agcagcagtg
     3061 acacttttac aatgagcatt attgacctga atcagcgttg tcttcgctcc aaatatttaa
     3121 tttctctttt tttgcttgtg cttcactttt tcttaaaagt tgttcatgtg tattgttagg
     3181 tttataaaca taagcaactt tagccaagcc ttgacgaact aaagcttcgt ttaccatttt
     3241 tccatcagca taaatatacg ctaagccacg tccatattta tcagttcttt gacctttgtc
     3301 aaactcgact tcaattttct ttgcattttc taccattttt ttcgtaaatg cacttgcttc
     3361 aggaccatat ttctctacac cttttttagg atgctttgtt tcaggtgtat caaccaataa
     3421 tagtctgaat gtcattggtt gacctttgta cattaattta accgtatcac catcaatcgc
     3481 tttaattaat gtcgcaggtt ctttatgtaa ttttttagtt gaagttgcac tatatactgt
     3541 tggatcttca gaaccacttc tatttacgcc attatctgtt tgtgatgcat ttgctgagct
     3601 acttagactt gaaactacaa ctaaagttaa cactaaacaa ctagtagcga aatagaaaaa
     3661 cctctttgcg tattgccctt tcgaaacatt actgatagcc atccctataa gtaatattga
     3721 aacgattgcc atacatatgc cagcacttaa taagtattct gtcataacta acacc
//
LOCUS       DAKTIP010000213         3744 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAKTIP010000213 DAKTIP010000000
VERSION     DAKTIP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.216"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            138..1088
                     /locus_tag="O0305_002455"
     CDS             138..1088
                     /locus_tag="O0305_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCY0275564.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTRIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            1237..2721
                     /locus_tag="O0305_002456"
     CDS             1237..2721
                     /locus_tag="O0305_002456"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCY0275565.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYDEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTTRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            2718..3677
                     /locus_tag="O0305_002457"
     CDS             2718..3677
                     /locus_tag="O0305_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCY0275566.1"
                     /translation="MRRLFSIGEALIDFIPNVTNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
BASE COUNT         1378 a          507 c          725 g         1134 t
ORIGIN      
        1 gttgatgttg ggacattttg ttatacaaaa gttttatttt gaaatctttt tatgaaagaa
       61 gcagaaatat tatttaaagc ggttacacat atgctaaaat aaggctaagt gtcacaaata
      121 atgataggtg aataagtatg aaaaatatat ctgatattgc caaattggca ggcgtttcaa
      181 aaagtacagt atctagattt ttaaataatg gatctgtcag taaaaaaaca agtgaaaaat
      241 taacaagaat tatagcagaa catgactatc aaccgaatca atttgctcaa agtttaagag
      301 cgagacaaac acaccttatt ggtgccatta tacctagaat gaattcatat gcagtagatg
      361 aaacaatcaa aggattggca aaacaatgcc aaaaatatga atcacaatta attttaaatt
      421 acacaggttt aaatatcgaa gcagaaatac aagcgcttga aacattagca cgcagtaaag
      481 tagatggtat tgttttaatg gctacagaca taacagagag acatattgaa gtcattaata
      541 aaatgaatgt accaatcgtt attgttggtc aacaacatga acaacttcat agtattgtgc
      601 atgatgatta taaagcaggt caaattatag gcgaatggat tggtcaacag ggatatcaac
      661 aagttgaagt gtttagtgta agtgaaaaag atattgcagt tggtatacat agaaaacgtg
      721 gtttacttga ccagttagct aaataccaaa ttaaacctaa tatacatgaa acaaatttta
      781 cttatgtgga agcacaaaaa gatgttgcaa atgttttgga aaatgtggag caagtagatg
      841 cggttgttgg agcaactgat acgattgcat tagctgccta taaatattat tctgataaaa
      901 aagatgttat gaaaccacat caaatatatg gttttggtgg tgacccaatg acacaattag
      961 tgtctccatc gataaaaaca attcattata attattttga agctggccaa tgcgcgatgg
     1021 aagagataca acagatgctt aaaaagcaag atatgccata tagcgtcaca gtagatgtta
     1081 atatttagac gctgtatttt ttaaaataaa tgtggaaccg ataccatata actataaatg
     1141 gataggttaa aagttaaaga acgtaggtaa aatttgctat aatagaatat aaattgttat
     1201 tagcataaat tataaaagga ggactgggta aatattatga ccgaatggac tagagaagaa
     1261 cgttatcaac gaatcgagga cgttgatact gagtatttta aaacattaaa acaacaagtt
     1321 gatcaatcaa aatttcgtca acaatttcat atacaaccag aaacaggctt attaaatgac
     1381 cccaacggac ttatttttta taaagggaag tattatgttt cacatcaatg gttcccatta
     1441 ggcgcagtac atggcttaaa gtattggtat aactacacga gtgatgactt aataaacttt
     1501 aaagctgaag ggccaatttt aaatccagat actaaatatg acagccatgg tgtatatagc
     1561 ggtagcgctt ttgaatataa cgggcattta tattatatgt acacaggaaa tcatcgagat
     1621 aatcattggc aacgacatgc gagtcagatg atcgcacgat tgaaagaaga cggttcagtt
     1681 gaaaagtttc caaagccagt aattagccaa caaccagaag gatatacgag tcattttaga
     1741 gatcctaaag tttttaaata tgatgagaaa tattatgcaa tcattggtgc acaaaataat
     1801 gatcagcaag gtcgattatt actttataat actgaagata taattaattg gcattattta
     1861 ggtgaaataa atacagagtt ggatgatttt ggatatatgt gggaatgccc agattacttt
     1921 aatgtagata atcaagatgt catacttatt tgtccacaag gtattgaacc aaaaggcgat
     1981 cagttcaaaa atatttatca aagtggttat atacttggaa agtttgatat tgaaaagtta
     2041 acatatgaac atgaaaattt tgtcgagctt gataatggtt ttgatttcta tgcacctcaa
     2101 acatttttag atgaaaaagg ccgacgagta ctaattggat ggatggggtt accggaaatc
     2161 gaatatccta ctgataatga aggatgggcc cattgcttaa caattcctcg tgtattaaat
     2221 gtagaaaatg gtcaacttaa gcaacgtcca tatccagcgt tggaaaaatt acgtcacaat
     2281 aaagagacag cattaggcta cgcaaataaa tttactcgaa aattacatcc gtatgaaggt
     2341 aaacagtatg aattaatcat agatattttg gataatgatg ctaccgaagt gtactttgaa
     2401 ttacgtacat ctaagacttc ttcaacatta attgcttata acaagcgtga aaataaaata
     2461 acattagatc gcagcgacag tggtttattg ccgacaaatg ttgaaggtac gacgcgtagt
     2521 acgatattag acacgccatt aaagcaatta caaatttttg ttgatacatc tagtatagaa
     2581 attttctgta atgatggaga acgtgttttg acatctcgaa ttttcccgac agaagacgca
     2641 ctaggtatta aaacatcaac agaatcaggg caagtatatt tacaatttac taaatatgat
     2701 ttaaaggatg aacataaatg agacgtctat tttcaatagg agaagcgtta attgatttta
     2761 ttccaaatgt tacaaatgcg aatttaaaag atgttcaaac atttacgaag caaattggtg
     2821 gcgcgccatg caatgtagct tgtacagttc aaaagttagg acaacaagcg tatatgatta
     2881 cacaattagg taatgatgca tttggagata gcattattga aacgatatca agtattggtg
     2941 tagatgtatc aaaagtgtat cgcacgaatg aagcaaacac tgcgttagct tttgtgagtt
     3001 tgactgaagc gggcgagcga gatttttctt tttatcgtaa gccctctgct gatatgttat
     3061 ttgaaccaag ttttgtgaat gatattgatg tgaatgaaaa tgatgtagta catttttgtt
     3121 ctgttgattt ggtcgatagt ccgatgagag atgcacatta tcaattgatt accaaaacat
     3181 taaatgcaaa tggtactgtc gtatttgatc ctaatgtaag attgccatta tgggataacg
     3241 ctgaagattt aagacaaact attcatacct ttttgccatt ggcgcatatt gtaaaagttt
     3301 cagatgaaga attagaattt attacaggta ttcatgatga aaatgaagcc attcaatcat
     3361 tatttacagg taatgttact gtagtcattt atacgaaggg agcagatggt gctgccgtat
     3421 atttgaaaaa tggtattaat cattatcata gtggctataa ggtaaaacct gttgatacaa
     3481 ctggggcagg ggatgcattt attggtgcag ttattagcag gattttagca accgatgtgt
     3541 taaatttaac tcagttattt gaaaatgaag gtgaggaaat tttagcgttt agcaatcgcg
     3601 ttgctgcaat agtgacaacg aagtatggtg ctattaatag tttgccaaca ttacaagagg
     3661 ttgaacaagc attttaaata attcatttat aaaacagtgt aggaaacaag ttgaatcttg
     3721 tgctacactg ttttttaaaa caaa
//
LOCUS       DAKTIP010000214         3741 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAKTIP010000214 DAKTIP010000000
VERSION     DAKTIP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.217"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(248..397)
                     /gene="rpmG"
                     /locus_tag="O0305_002458"
     CDS             complement(248..397)
                     /gene="rpmG"
                     /locus_tag="O0305_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCY0275567.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(488..2005)
                     /locus_tag="O0305_002459"
     CDS             complement(488..2005)
                     /locus_tag="O0305_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCY0275568.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2209..3663
                     /locus_tag="O0305_002460"
     CDS             2209..3663
                     /locus_tag="O0305_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCY0275569.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT         1152 a          677 c          570 g         1342 t
ORIGIN      
        1 ttctactata ttaaatactc tttcaagcat tcgctaacaa agtgacttta taaaatcagt
       61 aatctatttc cttctataaa tattctcaaa ttttaataaa gtaacaacta atataatcat
      121 atattgttct accgatgaaa ctcaatccat taatcactct tcacaaaaat ttcataaaaa
      181 agcgagctcc ccaatataat aggtaagccc gctttatttt caaatcgtcg tatttgaatg
      241 ataagaatta cttagtttca cgatgtaacg tatatttgtt taatcttggg caatattttt
      301 tcatttcaat acgctcagga ttattacgtt tatttttagt agtgatatag ttacgatcgc
      361 cacattctgt gcatgctaat gttacgttta cgcgcacgtt aaacaccctc tcttgttagg
      421 aatctttacg atttattatg atatcataaa ctgctcaact acgcaatata gaaactacat
      481 atcaaattta tttttcaaag ttttcgtatg tttcatcatt ttcagtttca agatcaatag
      541 aatttatatc aatacctaat gcttttgcaa cacctttacc atattctggg tcagctttgt
      601 aacaatgacg aatatgacgt cgtttaacat catccgttac gccttccatt gcatttgctg
      661 tatttgtaaa aattctttct ttagcgtcct ctgattgtaa tctaaacaat ttacctggtt
      721 gttcaaaata attatcatca tcttgacgtt gattatattc atagccgtat ccatcagttg
      781 ggaatggtgg ctttttatat tcaggttgag aatcaaattt accatggtta tttggataat
      841 aatgtgttcc tccaccttgg ttattgtcaa ctacgcgcat ttgaccatct ctactaaaag
      901 gacaaatatt ttcaatacca acacctttag gttggtttac aggaatctgc caatgattaa
      961 ctcctaatcg atatctttgc gcatcgccat atgagaataa acgcccttgc agcattttgt
     1021 ctggagaaaa atctaatcct ggaataatat tagttggtgc aaacgcagct tgttcaacat
     1081 ccataaagta attatctgga tttctattta attcaaactc tccaacttca attagaggat
     1141 actcatcgtg ataccatact tttgttaaat caaatggatt atctttatgg tttttagctt
     1201 gttcctcagt cattacttga atatacattg tccattttgg ataatcacct ttttcaatgg
     1261 cttcgaataa atcgcgttga gatgaatcac gatctgtagc tataatttca gcagcttctt
     1321 catcagttaa gttttcaata ccttgttgcg ttctaaaatg gaatttaacc caaacacgtt
     1381 caccagaatc attatacata gagtatgtgt gagaaccgaa cccatgcata tgacgtaaat
     1441 ctttaggaat ccctctatct gacattaaga tcgttacttg gtgcaatgct tctggaagac
     1501 ccgtccagaa atcccagtta ttttgtgcat ctctcatatt tgttctagga tctcgtttca
     1561 ccgcacgatt taaactaaca aataactttg gatctctaaa gaagaatact ggtgtgttat
     1621 tccctactaa atcccagttc ccttcttcag tgtagaactt taacgcaaat cctcgaatgt
     1681 cacgctccgc atcagcagca ccacgttctc ctgctacagt agagaaacgg gcaaacattt
     1741 cggtttgctt acctatttca gagaatattt tagcattcgt atattttgtt atatctttag
     1801 ttacagtaaa tgtcccaaat gcaccagaac ctttggcatg cattcgacgt tctggtatta
     1861 cttctctatc aaattgagac atttgctcta aaaagtaaat atcttgcatt aaaagaggtc
     1921 ccctaggccc tgctgtcata ctattttctc ggtctgatac tggatgccca aaaacaccag
     1981 ttaacttttt gtcttgttgt gacatagtca tccctccaca atttataata attataattt
     2041 aatacatatt ataatttaac cccatcaaaa ttacaattaa acatgctcaa gatgattaaa
     2101 aaatcagaaa attttaattt tttcgcttta cagtactaaa tagttattga atagtacact
     2161 taaaaagctt acaataatgg gtatatttaa atagatggga gactaaatat ggaagataat
     2221 aaaatgaacc gtagtcttaa ctcaagacac atttccatga ttgctatagg tggtgcaatt
     2281 gggactggtc tatttgtagc tactggtaat atcatttctc aagctggtcc tggaggcgct
     2341 atactcgctt atcttgttat tggtgtcatg ctatatttct taatgtcatc aattggagag
     2401 ttggcaacat tttatccagt atcaggttca ttcagctctt attcaacacg ctttattgac
     2461 tcatctcttg gctttaccat gggatggttg tattgggcat tgtggtcatt agttacaagt
     2521 gttgatgtca tagtagcgtc aaatgtgctt tatttctggg acacatttaa atttttccac
     2581 cccattactt ggagcttaat ctttattaca attttactat tattaaacat tttttctgta
     2641 aaatcatttg gagaaactga gttttggtta tcattgatta aagtgttaac aattatcgta
     2701 ttcgttattt ttggcttttt aatgattttc ggtatcttag gtggtcatac atatggattt
     2761 gaaaactata caaaaggcca agcaccgttt gttggtggta tctctggttt cttaggcgta
     2821 ttattagtcg ccggattttc ggttggtggt acagaagtag tagcagtaac tgctggtgaa
     2881 tcagatgacc ctaaaaagtc tatgcctaag gcaattaaac aagtattttg gcgtattctt
     2941 ttattctatg tcttatcaat tgcagtaatt ggtgcaatta ttccgtacac agatccatca
     3001 ttattaagag caagtagttc aataagtcaa agcccattta caattgtatt cgatagagta
     3061 ggcatagcct ttgcagcatc agtaatcaac gcggttattt taacttcatt attatccgct
     3121 gcaaattcag gtgtttatac aacaggcaga atgttgtatt ccttaagttc agacaaaaaa
     3181 gcaccccaat ttttaagtaa attaaacaag acaactaagt tacctttaag agcattatta
     3241 actacttatg cagtcgttgt tattgttatt atttatgcaa actttaattc aaatgccgtt
     3301 tttaatttac ttgaaattat tggttcaatg attatagttg tttggggatc aagcatttgg
     3361 tcacaaatac gattgcgaca agctattaaa aaacaaggtc aagaccctaa taaggtccta
     3421 ccatataaag caccttttta tccattagga ccaatcattg tcatcactac actattattc
     3481 ttgctatttg gtggctcagt tgaatatatt ttaaaagatc aatggttaaa tgcttttaaa
     3541 aactttttac ctttaatcat tctagcgttg atttacttta ttcataaaat cattcacaaa
     3601 acaaaatttg taaagctaga aacaattaat ttaaaaccac acgattatga caatcaaaaa
     3661 taaataaatt ctcatacggc agatgcttta gattatctat agcatttgcc gctttttaat
     3721 ggtgtaaagc agatatactt t
//
LOCUS       DAKTIP010000215         3713 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAKTIP010000215 DAKTIP010000000
VERSION     DAKTIP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.218"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(153..725)
                     /gene="raiA"
                     /locus_tag="O0305_002461"
     CDS             complement(153..725)
                     /gene="raiA"
                     /locus_tag="O0305_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCY0275570.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(786..1460)
                     /locus_tag="O0305_002462"
     CDS             complement(786..1460)
                     /locus_tag="O0305_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCY0275571.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(1453..2535)
                     /locus_tag="O0305_002463"
     CDS             complement(1453..2535)
                     /locus_tag="O0305_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCY0275572.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYR
                     ILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQRMNKLALKRGWIDE"
     gene            complement(2899..>3713)
                     /gene="fakB1"
                     /locus_tag="O0305_002464"
     CDS             complement(2899..>3713)
                     /gene="fakB1"
                     /locus_tag="O0305_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCY0275573.1"
                     /translation="DKYNIQIAPLSVTFDDGKNFTESNEIAIEEFYNKMASSQTIPTT
                     SQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSYQAGEMVEGVNVHAFDSKL
                     AAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYLIVDDLKNLQKSGRITGAQ
                     AWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKVLDIVKDFEEVTLFVINGD
                     HFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGLGLGYVGRKIRLT"
BASE COUNT         1147 a          674 c          500 g         1392 t
ORIGIN      
        1 aacttaacat tcaaatatat acatccatta acattagcat agtcactatg tttcattcaa
       61 caaattacat tatcgaacta tgaaatagtc ataatttgct tttggagtat aaaaaagcac
      121 ttgtgcaaaa acacaagtgc tttaaactta atttattgtt cactagtttg aatcaagcca
      181 tatttaccgt ctttacggcg gtaaacgata cttgttccat cagtttctct gtctgtgaat
      241 acaaagaagt catgacctaa tagattcatt tgtaataccg cttcttctga atccattggt
      301 tttaagctga attcttttga acgaataatt tctatctcgt tatcatcgta agcgtcatta
      361 tcaacttgtg tttcttgcat ttcttgtaat tcggcaacaa acacttcttg atctcctcga
      421 tcacggctct tacgattaat acgtgtttta tattttcgaa cttgtctttc aagtttatta
      481 ttaattaaat caatacctgc gtataaatca tcgtttcgct cttcagctct taacgtaaca
      541 tttttcaatg gaattgttac ttcaatttta gtagctgaat ttgaataagt tttaacttta
      601 acatgcgcca ctgcatttgg tacgtcatta aaataacgtt ccaacttacc aattttttcc
      661 tcaatatagt tgcgaatagc atctgtgata gtgaggttat ctccatgaat ttcaaatcta
      721 atcatagtaa tctctcctta aacctcttta ttggtaactc tttattatat tttaacattt
      781 ttacgctatc gtgcaaacgc aaacactttg aattttctga tatttttagc atataattta
      841 caccctgcac gatgaattgt taatccagtt gtataaatat cgtcaacgag taatatttcc
      901 ttaccattta aatctaattc cgtatcaata ataaatggat tttcatctgc cagacgctct
      961 ttcttagtta aatgagactg ttttggtcta tttgacatct ttaaaatctt atcaaagcga
     1021 atccctttag cttttagtac tgcttctacc gggttaaatg ttctagataa atcatgtgcc
     1081 ggcgaagaag gaattggcac aatatagtca taagatgttt gtggtatttc aatcaaatgt
     1141 gccaataatt cacataaata atagtctttc aaaaatttat actgatgtat catctctttc
     1201 attaaaccgt catattgaaa ttgacaatat aattgttcca ttaaattaaa gtgtgccgat
     1261 agaaacttgc agtctaaaca atacgcttca tcttgattta agtgttttaa gcaccttgaa
     1321 catcgccttg ctttaatatc aagtttaata ttgtcccaat tctctttgca tctgtcacat
     1381 aatctattag gtttcttgaa caaattataa atggttatat tttcatataa cttagcacca
     1441 caactcaaac aattattcat caatccaacc tctttttaat gctaatttgt tcatcctttg
     1501 aatctctttt ttagcttgaa tcatattcat acttacccct tcatgaaaaa acaatacttt
     1561 tccagtagga cattctaatt ttcgtccaac acgtccagca atttgtatta aagcctcttg
     1621 agtatattga tgtgcatcga taacaacaac atccaaattt gccattgtaa atccacgttc
     1681 taatattgtc gtagtaaaaa tgacatcgaa atgtccattc cttaattgtt caactttttc
     1741 aaagcgaaaa acatcctcgc tatggacgta tgttaattta gtaattttct gcttataaac
     1801 cgaaaatgtt ttaatcattg tttctatatt gttaaaaaac accagtgtat aacgttgatt
     1861 attaatttga tcttgtaaaa ttcggtataa cattttctga atcttcttat tattaagttt
     1921 gaaataacga tatttaggaa ctggaagtga ttttttatga aagcgagctg gcaatttaat
     1981 tatattttcg tggggaatct ctgacagaag ttgtttcggt ggtgttgctg tcatataaat
     2041 tgttgcatgt tcaactttag aagatgactt caatgcttgt tgtaaatttt tatccattga
     2101 taaaggaaag gcatcgactt catcaataaa aatagtatca aagtgctgtt tgaatcggta
     2161 aagttgatgc actgtgcata caacaaaatg cccttcaaat tgttgtcttg attgctggtg
     2221 tagtatgtct atatcttcat taagaaatgc gtctttaata cgtttactaa tttctacaac
     2281 aacatctaca cgtggtgaca caatagctat attatctccc tgtattcttg catattgaat
     2341 gccttgaaac atcatttctg tcttacctgc acctgttacg gcatataaca aaatcgtttg
     2401 tctctttcta atggctcgaa caatatgttc tgaggcataa gactgctgtt ccgacaattc
     2461 aaatgagaga tgataatatg cttgtgatga actttgcgaa ctctctgtta ttttatatct
     2521 tgttacatta tccattcgac ccagtgatat acaatttctg caatatacaa ttttcttgga
     2581 atgtactgag gaatcatact gataaaaatg ctttggattt gtactttcac attgaacaca
     2641 ataatagttt gaattttgtt tgatgacacc tgttacttct tttttaattt tttcattagt
     2701 aagtgaagta tgaggatgaa ttaatttccc gtaatgttta atgcttaaaa cctcatttca
     2761 acgtaaaaaa gagcctaata ttataatgat tttcttatat ctttagcatt ttggctttca
     2821 aatgatgaac atttaaagcc atgtatacga gagataattt ccaattacaa tatagactct
     2881 tttattaaaa ttttataatt atgttaatct tatttttctg ccaacatagc ctaaacctaa
     2941 tccaccagaa cctaaatgtg ctgcaacaac tggaccaaac tcagagtatg ctacttgata
     3001 agctgaagga caatcatctt gtaacttttt gtataacgct tgaccatctt cgaaatgatc
     3061 tccatttatg acaaataaag ttacttcttc aaagtctttt acaatatcta atactttctt
     3121 ttctaatgtt tgaatggcac gctttttagt acgaactttt tcttctggta taatcttgcc
     3181 atcttcaaac ttaagaactg gcttcatttt caataatgtt ccaacccatg cttgagcacc
     3241 agtaattcga ccactttttt gtaaattctt taagtcatca acaattaaat atgcacctgt
     3301 gtgttcacgc atattagtta aatcatcaat aatttgctgt ggctcgtatc cttcttcaac
     3361 catttcaata gcacgtaata cataacatcc ttcaatcatt gctgcaagct tactatcaaa
     3421 tgcatgtaca ttaacacctt caaccatttc ccctgcttga taactagatt gataacttcc
     3481 actaatccca cttgataagc aaatgacaat gatatctgtg taaccttgat ctcttaacat
     3541 ttcatattta gtaatccatt cgccaattgc tggttggctt gttgttggaa tcgtttgaga
     3601 cgatgccatt ttattataaa attcttcaat tgctatttca ttactttctg taaagttctt
     3661 cccatcatca aaagtcacac ttaatggcgc tatttgaata ttatatttat cga
//
LOCUS       DAKTIP010000216         3710 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.219, whole genome shotgun sequence.
ACCESSION   DAKTIP010000216 DAKTIP010000000
VERSION     DAKTIP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.219"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..387
                     /locus_tag="O0305_002465"
                     /pseudo
     CDS             1..387
                     /locus_tag="O0305_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            447..1247
                     /gene="lpl4"
                     /locus_tag="O0305_002466"
     CDS             447..1247
                     /gene="lpl4"
                     /locus_tag="O0305_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl4"
                     /protein_id="HCY0275574.1"
                     /translation="MGYLKRLVLYIVIMVMSVFIIGCDKSSDTAEKSKEDSKETQIKK
                     SFAKTLDMYSIKNLEELYDKEGYRDGEFEKGDKGMWTIYTDFAKSNKPGELSNEGMVL
                     YLDRNTRTAKGYYFVRTFYRKDKLPDRKNYKVEMKNNKIILLDKVEDKKLKQKIENFK
                     FFSQYANLKELKNYSNGDVSINENVPSYDVKYKMSNKDENVKQLRSRYNIPTDKSPVL
                     KMHIDGNLKGSSVGDRKLEIDFSKRENSHLSVIDSLDYQPAKTNKDDE"
     gene            1266..2036
                     /gene="lpl5"
                     /locus_tag="O0305_002467"
     CDS             1266..2036
                     /gene="lpl5"
                     /locus_tag="O0305_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCY0275575.1"
                     /translation="MKSIKRIGLCISLLILSIFVTSCDGDNKITGDSKETQIKKSFSK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKGNKSDELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLPDRKNYKVEMKNNKIILLDKVEDPNLKKRIENFKFFGQ
                     YANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQLRSRYNIPTDKAPMLKMHI
                     DGDLKGSSVGYKRLEIDFSKEGRDISVIDYLSYKPAKK"
     gene            2068..2859
                     /gene="lpl6"
                     /locus_tag="O0305_002468"
     CDS             2068..2859
                     /gene="lpl6"
                     /locus_tag="O0305_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl6"
                     /protein_id="HCY0275576.1"
                     /translation="MEYLKRLALLISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDFYGKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTRGMQL
                     YINRNTETAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIKKEIE
                     NFKFFVQYGNFKNFEKYNNGEFSYNPEAPIYSAKYQLHNDDYNVRQLRKRYDISTKET
                     PKLLLKGGGDLKNSSVGQNDIEFTFVERKGENIYFNDSVEFIPSK"
     gene            2929..3705
                     /gene="lpl7"
                     /locus_tag="O0305_002469"
     CDS             2929..3705
                     /gene="lpl7"
                     /locus_tag="O0305_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl7"
                     /protein_id="HCY0275577.1"
                     /translation="MKCFQKLYIFILILIVLMAGCESNKITGDSKETQIKKSFAKTLD
                     VYPTKNLEDFYDKEGYRDGEFKKGDKGKWVIRSEMTTELKNENMVSKGMVIRLNRNSR
                     TCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIITLKPIDDEKVKKEIEEFKFFVQ
                     YGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNITTKKAPKLLLKG
                     SGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
BASE COUNT         1583 a          381 c          709 g         1037 t
ORIGIN      
        1 gaaattggtg aataatagga tagttttgat aaaagatgtt aaagacaaaa agttaaaaaa
       61 atgaaataga gtcgtttgaa ttattttcac aatatggaaa ctttaatcat tttgatcgga
      121 atgagattac taatatttca tataatccta atgctcccaa ttactctgca gaatataaaa
      181 tgaagaaaaa tgacagaaac attcaacagt tgaaaaagag atttaatcta aaaactagca
      241 agacaccaaa attattgttt aagggatctg gagatataaa ggggtcttct gtaggatata
      301 aggaaataga aatcatattt agtagaagta aagaagaaag catttattat gttgacagcg
      361 ttgagttcgt tccaagtaac aaaataacaa aaagttgata ttagtcaaca aaagcaaata
      421 ttgagaataa agagagttgt gaaatgatgg gttatttaaa aagacttgta ttgtatatag
      481 ttattatggt tatgagtgtt tttataatag gttgtgataa atcaagcgat actgcagaaa
      541 aatcaaaaga agactcgaaa gaaacacaaa tcaaaaagag ttttgcgaaa acgttagata
      601 tgtattcaat taagaatctc gaggaactat atgacaaaga aggctatcga gatggcgaat
      661 ttgaaaaagg tgacaaaggg atgtggacga tatatacaga tttcgccaaa agtaataaac
      721 cgggtgaact aagtaatgaa ggcatggtct tgtacttaga tagaaacaca cgcacagcta
      781 aggggtacta ctttgtcagg acgttttatc gcaaggataa attacctgat agaaaaaatt
      841 ataaagttga aatgaaaaat aataaaatca ttttattaga taaagtagaa gataaaaagc
      901 ttaaacaaaa aatagaaaat tttaaatttt tcagtcaata tgcaaatctt aaagaattga
      961 aaaactacag caatggtgat gtctcaatta atgagaatgt tccaagttat gacgtgaaat
     1021 ataaaatgag taataaagat gaaaatgtta agcaattaag aagtcgttat aatattccta
     1081 ctgataaatc accggtatta aaaatgcata ttgatggtaa tttgaaagga agttccgttg
     1141 gagataggaa gttagaaatt gatttttcga aacgtgaaaa tagccattta tcagtaatag
     1201 attctttgga ttaccagcca gcgaaaacta ataaagatga tgaataatga ggatggtgtg
     1261 taacaatgaa gtctataaaa aggattggat tgtgcataag tctgttgatt ttaagcattt
     1321 ttgtaacatc ttgcgatggt gataataaga tcactggaga ttcaaaagaa acacaaatca
     1381 aaaagagctt ttcgaaaacg ttagatatgt atccaattaa gaatctcgag gacttatatg
     1441 ataaagaagg ataccgtgat ggcgaattta aaaagggcga caaaggaatg tggactatat
     1501 atacagattt tgctaaaggc aataaatcag acgaattgga tgatgaaggt atggttttaa
     1561 atctggatag aaatactcga acggctaagg gatattattt tgttaagaaa ttttatgaaa
     1621 aggataaatt acctgataga aaaaattata aagttgaaat gaaaaataat aaaattatct
     1681 tattagacaa ggtagaagat ccaaatctaa aaaagagaat agaaaacttt aaatttttcg
     1741 gacaatatgc aaattttaag gatttggaaa attacaacaa tggcgacgtg tcaataaatt
     1801 ggaatgttcc aagttatgac gtggaatata aaatgagcaa taaagatgaa aatgttaaac
     1861 aattaagaag tcgttataac attcctactg ataaagctcc aatgttaaaa atgcatattg
     1921 acggggactt aaaaggtagt tctgttggat ataaaaggtt agaaatagat ttttcaaaag
     1981 aaggtaggga tatttcagtc attgattatt taagttataa gccagcgaaa aaatagtact
     2041 gataattaaa tattagggtg tgaaggaatg gaatatctaa aaaggcttgc attgttaata
     2101 agtgttatta ttttgaccat ttttataatg ggttgtgata gtcaaagcga tactgcagaa
     2161 aatccaaaag aaggttcaaa agaagcacaa attaaaaaga gtttttcgaa aacgttagat
     2221 atgtatccaa ttaagaatct cgaggatttt tatggcaaag aaggatatcg agatggcgaa
     2281 tttaaaaaag atgataaagg tacttggcta attagatccg aaatagttaa acagccaaag
     2341 ggcaaagtga tgaaaacaag aggtatgcaa ttatatatta atagaaatac cgaaacagcc
     2401 aaaggtttct ttgttttgaa agaaataagt gaaaataata atcgtgtaaa taaagataag
     2461 gaggaaaaat acgaagtgaa aatggtagga aataaaatta ttcctactga acaaattaat
     2521 gacgagaaaa taaaaaaaga aattgaaaac ttcaagtttt ttgtgcaata tggaaacttt
     2581 aaaaatttcg aaaaatacaa caatggtgag ttttcatata atcctgaagc accaatttat
     2641 tcggctaaat atcaattaca caacgatgat tacaatgtaa ggcaactacg taaaagatat
     2701 gacatttcaa caaaagaaac accgaagtta cttttgaaag gtggaggaga tttaaaaaat
     2761 tcctcagttg gtcaaaacga tattgaattt acttttgttg aaagaaaagg tgagaatatt
     2821 tattttaacg atagtgttga attcatacca agtaagtaaa gttaaattaa aatgtgtggt
     2881 tagatgaaat gatcaaatat caaaaaatgt gcaaagagat gtgacaagat gaagtgtttt
     2941 cagaaattat acatatttat attaatttta atcgtattaa tggcaggatg cgaaagtaat
     3001 aagatcactg gagattcgaa agaaacacag atcaaaaaga gctttgcgaa aacattagat
     3061 gtatacccta cgaaaaatct agaagatttt tatgacaaag aaggatatcg agatggcgaa
     3121 tttaaaaagg gtgacaaagg gaagtgggtt attagatctg aaatgacaac agaactgaaa
     3181 aatgaaaata tggtatctaa aggtatggtc atacgtttaa atagaaatag tagaacatgc
     3241 actggtgaat attttgtcag gatagttaaa gaagacagtg agggcaaggt atatagtgat
     3301 gaacgaaaat atccagtgaa aatggaaaat aataaaatca ttacattaaa accaatcgat
     3361 gatgaaaaag taaaaaaaga aattgaagaa tttaaattct tcgtacaata cgggaatttc
     3421 aaagaattgg aaaactataa agacggagaa gtgacatata acccagaagc accaatatac
     3481 tctgcacaat atcaattgaa aaatagtgat tataatgtag aacaactacg taagcgatat
     3541 aatataacga cgaaaaaagc gcctaaatta ttattgaagg gttcaggtaa tttaaaaggc
     3601 tcatcagttg gatataaaaa tattgaattt acctttgttg aaaataagga agaaaatatt
     3661 tacttcacag atagtattaa tttcaaccca agtgaggata aataagtata
//
LOCUS       DAKTIP010000217         3685 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.220, whole genome shotgun sequence.
ACCESSION   DAKTIP010000217 DAKTIP010000000
VERSION     DAKTIP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.220"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            96..1622
                     /gene="spa"
                     /locus_tag="O0305_002470"
     CDS             96..1622
                     /gene="spa"
                     /locus_tag="O0305_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCY0275578.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDNNKPGKEDGNKPGKEDNKKPGKEDGNKPGKEDNKKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1951..3543)
                     /locus_tag="O0305_002471"
     CDS             complement(1951..3543)
                     /locus_tag="O0305_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCY0275579.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISVDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVIKLPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICVWTFILTLIF"
BASE COUNT         1393 a          714 c          570 g         1008 t
ORIGIN      
        1 acctttaaat ttaattataa atatagattt tagtattgca atacataatt cgttatatta
       61 tgatgacttt acaaatacat acagggggta ttaatttgaa aaagaaaaac atttattcaa
      121 ttcgtaaact aggtgtaggt attgcatctg taactttagg tacattactt atatctggtg
      181 gcgtaacacc tgctgcaaat gctgcgcaac acgatgaagc tcaacaaaat gctttttatc
      241 aagtcttaaa tatgcctaac ttaaatgctg atcaacgcaa tggttttatc caaagcctta
      301 aagatgatcc aagccaaagt gctaacgttt taggtgaagc tcaaaaactt aatgactctc
      361 aagctccaaa agctgatgcg caacaaaata acttcaacaa agatcaacaa agcgccttct
      421 atgaaatctt gaacatgcct aacttaaacg aagcgcaacg taacggcttc attcaaagtc
      481 ttaaagacga cccaagccaa agcactaacg ttttaggtga agctaaaaaa ttaaacgaat
      541 ctcaagcacc gaaagctgat aacaatttca acaaagaaca acaaaatgct ttctatgaaa
      601 tcttgaatat gcctaactta aacgaagaac aacgcaatgg tttcatccaa agcttaaaag
      661 atgacccaag ccaaagtgct aacctattgt cagaagctaa aaagttaaat gaatctcaag
      721 caccgaaagc ggataacaaa ttcaacaaag aacaacaaaa tgctttctat gaaatcttac
      781 atttacctaa cttaaacgaa gaacaacgca atggtttcat ccaaagccta aaagatgacc
      841 caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caagcaccaa
      901 aagctgacaa caaattcaac aaagaacaac aaaatgcttt ctatgaaatt ttacatttac
      961 ctaacttaac tgaagaacaa cgtaacggct tcatccaaag ccttaaagac gatccttcag
     1021 tgagcaaaga aattttagca gaagctaaaa agctaaacga tgctcaagca ccaaaagagg
     1081 aagacaataa caagcctggc aaagaagaca ataacaagcc tggcaaagaa gacaacaaca
     1141 agcctggtaa agaagacaac aacaagcctg gcaaagaaga cggcaacaag cctggtaaag
     1201 aagacaacaa aaaacctggt aaagaagatg gcaacaagcc tggtaaagaa gacaacaaaa
     1261 aacctggtaa agaagacggc aacaagcctg gcaaagaaga tggcaacaaa cctggtaaag
     1321 aagatggtaa cggagtacat gtcgttaaac ctggtgatac agtaaatgac attgcaaaag
     1381 caaacggcac tactgctgac aaaattgctg cagataacaa attagctgat aaaaacatga
     1441 tcaaacctgg tcaagaactt gttgttgata agaagcaacc agcaaaccat gcagatgcta
     1501 acaaagctca agcattacca gaaactggtg aagaaaatcc attcatcggt acaactgtat
     1561 ttggtggatt atcattagcc ttaggtgcag cgttattagc tggacgtcgt cgcgaactat
     1621 aaaaacaaac aatacacaac gatagatatc attttatcca aaccaatttt aacttatata
     1681 cgttgattaa cacattctta tttgaaatga taagaatcat ctaaatgcac gagcaacatc
     1741 ttttgttgct cagtgcattt tttattttac ttacttttct aaacaacttc tgaaacgcct
     1801 caacactttc tactctgatt acatatatga catttttagg cattaaaaaa tcgaactaga
     1861 caagatgctc attgcatttc gtactagttc gattcatgaa taattagatt taaaatgtca
     1921 tttgaatcca agtgacaaca ttatttatat ttagaatatt aacgttagta taaacgtcca
     1981 aacacaaata aaagcaacaa atataatact gtattttaac gtcattttta ataatgcaga
     2041 ttcttcacca acttttttaa cagctgcagt cgcaatggca attgattgtg gtgaaataag
     2101 tttcgctgct acaccacctg cagtgttagc tgccacaagt aatgaaccgc ttgttgaaat
     2161 ttgttgtgcc actgtcgctt gaataggtgc gaataaagta ttgttattta caactgaacc
     2221 agtcataaac acaccaatcc aacctaatac tggagagaat aatgggaaaa ttgctcccgc
     2281 tttagcaata ccttgtccaa ttgctacagt caaaccaccg tatgtcataa ctttagcaat
     2341 agctaggata gctgaaattg taaggatcgg taaccataat tctttaattg cttcgaccaa
     2401 taaagcacct gcacttttcc attttaactt cgtaattaaa attgtaataa ttactgttaa
     2461 taaaatcgct gtcccagttg caccaattaa atcgagacgc aacgcaattc ctttaggcga
     2521 taaatcactc acagtatttg gaattggcaa ttttattact aaactttcaa gtgcacctcc
     2581 aggttggaat aattttttga agaatggtgc actccatact aatacaaagg cagttaaaat
     2641 tacgaacgga ctccaagcaa agacaatttc tttaggcgtt cgtttttgaa ttttatgttc
     2701 agacgcttcc aatctgaaaa tgtttttcgg tttaaattta cgacaaacaa atgctaacac
     2761 caccattgtt gctagtgatg gaataatgtc tgctagttct ggaccatgga atattgttaa
     2821 taataattgt aatccagtat atgtaccact cactgttaaa atgacaggta aaatttcttt
     2881 aatacctttc ataccatcta caatgaatac taaaacaaat ggaataataa agtttaaaat
     2941 tggaagtgtt aatgctgagt atctcgcaac atctaatgtt gtaacgcctc cacttaagtt
     3001 aaacgtatca ataatactaa ctggtaaacc aattgcacca aaggcacccg ccgcaccatt
     3061 agcaattaaa cataacatcg ctgcttttaa tggttcaaat ccaagttgaa ttaataatac
     3121 tgcacaaatc gcaattggca caccaaatcc tgctgcacct tctaaaaatg cgttgaaaca
     3181 aaatccaatt aataatagtt ggattctttg gtccactgaa atacttgcaa tactatcttg
     3241 aataatagaa aattgtcctg ttttaataga aactttatat aaccaaactg ccattaaaac
     3301 gatatatcct attgggaaaa taccggcaac aacgccttct gtaatcgcac ctgctgatac
     3361 acgcgctggt aattcaaata caaataaagc cacaatcaat gtaacaacca aagttgtcaa
     3421 tgctgcataa atgcctttca ttttaaaaac ggttaagcat aataaaaata aaataatagg
     3481 tactgctgca actaaggctg ataatccgac attatcgaat ggatttacag taagtagtgt
     3541 cataatgact ccctctcttt atataaaata tttatcattc tgattaatct acaacctatt
     3601 tcaacttata ttttgcgatg atcacatatt taaaatgtaa cactcctata tgtgacaggc
     3661 aatcgaattt ttacaaaaag ttcac
//
LOCUS       DAKTIP010000218         3574 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.221, whole genome shotgun sequence.
ACCESSION   DAKTIP010000218 DAKTIP010000000
VERSION     DAKTIP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.221"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..297
                     /locus_tag="O0305_002472"
     CDS             <1..297
                     /locus_tag="O0305_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0275580.1"
                     /translation="PQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLNNLIEQDH
                     RHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIMLAS"
     gene            355..732
                     /locus_tag="O0305_002473"
                     /pseudo
     CDS             355..732
                     /locus_tag="O0305_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            729..1610
                     /locus_tag="O0305_002474"
                     /pseudo
     CDS             729..1610
                     /locus_tag="O0305_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1726..2655
                     /locus_tag="O0305_002475"
     CDS             1726..2655
                     /locus_tag="O0305_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275581.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2648..3385
                     /locus_tag="O0305_002476"
     CDS             2648..3385
                     /locus_tag="O0305_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275582.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3399..>3574
                     /locus_tag="O0305_002477"
     CDS             3399..>3574
                     /locus_tag="O0305_002477"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="BacA; phosphatase activity in Escherichia coli not
                     kinase; involved in bacitracin resistance as bacitracin
                     supposedly sequesters undecaprenyl disphosphate which
                     reduces the pool of lipid carrier available to the cell;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase"
                     /protein_id="HCY0275583.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLG"
BASE COUNT         1308 a          491 c          632 g         1143 t
ORIGIN      
        1 cctcaaaagg tagttacaga tcaggcacct tcaacgaagg cagcaatggc taaaataatt
       61 aaagctttta aacttaaacc tgactgtcat tgtacatcga aatatctgaa taacctcatt
      121 gagcaagatc accgtcatat taaagtaaga aagacaagat atcaaagtat caatacggca
      181 aagaatactt taaaaggtat tgaatgtatt tacgctctat ataaaaagaa ccgtaggtct
      241 cttcagatct acggattttc cccatgccac gaaattagca tcatgctagc aagttaaacg
      301 aacactgaca tgatatatta gtgattagct atatttttta ctttgcaaca gaaccagcca
      361 tttaatatga ctgttacaat tactaagatt caagcactat tacgccgcac gtatgatttc
      421 accactcaag ttaataattt aacagtagat gattgcgagt tactacttga tgcagcaaag
      481 cttaaattta atgaagaaac catagacctg acacacactg aattgcaaat catgcatagc
      541 ttattccaga acaagggcaa attcgtcagt cgtaatgcgt taatcgaaaa atgctgggaa
      601 tcagaacact tcattgatga caatacgctt gctgtcaaca ttacacgttt acgcaaaaag
      661 ctacaaaaac taggacttac agattttatc gaaacgaaga aaaatgtcgg atatcgtgta
      721 ggtgacgtat gaatttcttg aaatcaatcc aacgtgaaat ttatattgtc atccttactt
      781 gtgctttatt ctactttata ttattcatgt ataatgtgcc gatgcaagca tatatgattg
      841 gtctaggtat tgtattgttt gtttcaatga tttactgggg tatccattac atgaacttta
      901 aaaaagaata aacactacag gaacagaacg aagatttaag aatcgaaaac cataaacttc
      961 gtaatgactt tattaactac caaagtgagt tagagtcata ttttttaatg tgggtgcatc
     1021 aaatcaagac accgattaca gcatcaaagt tactgcttga agatcctgat gaacacgtga
     1081 tttctaacgt gcgtaatgaa gtcttacaaa ttgataatta cacgaatctt gcactgaact
     1141 atttgaaact catgaaccaa aagacataca tggtctttat ggaagtgagt gtagatgaat
     1201 taattcgacc actgattaaa cgttatgcga tacagtttat tcatcatcaa acaaagttgc
     1261 attatgagaa aagtgaagcg gttatattaa cagatgccaa atgggcatca attatgattg
     1321 agcaaatatt aaacaatgca ttaaaatatg cacgtggcaa agatgtgtgg attacgtttg
     1381 acgaaaatac aaagaaatta tcaatccgag ataacggtat cggcattaat gcgagtgatt
     1441 taccgaaaat ctttgataaa ggtttctcgg gctataacgg tagaataagt gagaaatcaa
     1501 gcggtattgg tttatatatt gtcagcacaa tctcaaagcg cttaggccat aaagtaagtg
     1561 ctacatctac attaaacgat ggttcaatat tcaccattca atttaaataa ctccaatctt
     1621 tcatttttgt aagattagcg gctgtttttg taagaataga taaaggcagc cttttctaat
     1681 cgcgcgtaag ataaaggtaa caacaaacta agaaaggtgc cagaggtgaa aaatttgaat
     1741 aatataattg aaataaataa cttaacaaag caatatggaa gtcagatagc agttaataat
     1801 ttgagtataa ctgttgaaag tggacaaatt tatggttttt taggaagaaa tggtgcaggc
     1861 aaaacaacta ctataagaat gcttttaggc ttaattaaac ctacgaaagg taatattaaa
     1921 atatttgaaa aagagttcga aagtaataaa actgaaattt taaaaaatat tggtgctatg
     1981 gttgaaacgc caggttttta tagtaatttg acagctaaag agaacttaac tataaatgct
     2041 aaaatattag gtatacaaaa agaaaatgca attgaagaag tgttagaaat agtaggacta
     2101 gcttctgaaa aagtaaaatt gtttaaaaat tattctttag gtatgaagca acgtttaggg
     2161 attgcgagag ctttattgca caatcccgaa ttattgatac ttgatgagcc gacgaatgga
     2221 ttagatccta ttggcataaa agaaattaga aagttgatta aggatctggc tcaatctaga
     2281 aatataacca ttttaatttc aagtcatata ttgtcagaaa tagagcagtt agtagacaag
     2341 gtgggtatta ttcataacgg agttttgtta gaagaaatta tgtatgaaga tttaaaaaaa
     2401 aagaacagac aatttataag tatatcagta tcagatgaag aaaaagttgc atgtttgttg
     2461 gaaactaact tcggaattta tgactataaa attaaagaaa atggcgaatt taaaatttat
     2521 tcacatattg atagacaaca agaaattaat agattattag tattaaacaa tattgatgtt
     2581 ttccagatga gagtgataga agataatcta gaaacttatt tcgaagactt agtaggaggt
     2641 ggcacaattg gataatctac tccgtacaga atttattaag ttaaaaagat catatatgtt
     2701 tctaataagt attttaggta catgcgtagc accttttatg gtggttgtag cttcttttat
     2761 tagtatgcgg acgaaagtac cgactcctaa tattgtattt aaacagttat ttgaagaagt
     2821 cagtttatat catatgctaa ttattggacc tcctctatat ggggttataa tagcctattt
     2881 gtttggaaga gaatatatag aagatacttt aaaaaactta ttaattatac ctatatctag
     2941 agaaagtttt ttagtaagta aaataatatt attattcttg tggattcaaa cactatctgt
     3001 tattgcatgg ttgctaactt taatgctagg tatgctattg cattttcaag ggttaagcca
     3061 gagtttaata attgattcat tctttacatt tgttattggc ggagtgttat tatggatatt
     3121 atctccacca ataattattt tgtcgatata ttttaaaaat tatgttccca caattgtttt
     3181 aaccataata attactttga taaatttaat gactgctaat tctgaacata gaggattatt
     3241 tccttgggct gcagttggag acatatctag aggaacatta cctaatactt atccatttct
     3301 attatcttta attatcataa ttgttacatc tatattcagt gtaattatat tattctcata
     3361 ctttaaaaaa actgatataa gttaaaagga ggagaaaaat gggttttata gagcagatta
     3421 aagcatttat tttaggtgta gttgaagggt tgacagaatt tgctccagta tcatcaacag
     3481 gtcatatgat tattgttgat gatatgtggt taaacactgc tgacgattta ggaagtgcgt
     3541 ctgcaactac ttttaaggtg gtaatacagt tagg
//
LOCUS       DAKTIP010000219         3567 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.222, whole genome shotgun sequence.
ACCESSION   DAKTIP010000219 DAKTIP010000000
VERSION     DAKTIP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.222"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..368
                     /locus_tag="O0305_002478"
     CDS             <1..368
                     /locus_tag="O0305_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0275584.1"
                     /translation="AYNSMRMWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFK
                     THRMIHKFKINSQGLTSDTWNLKYKQLKNINIDIPVLEEQEKIGDFFKKMDILISKQK
                     MKIEILEKEKQSFLQKMFL"
     gene            751..1428
                     /locus_tag="O0305_002479"
     CDS             751..1428
                     /locus_tag="O0305_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCY0275585.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLS
                     EYYNRPFFEYTNQSGYKEEGKVTFTPNYQLIDVTLTGNEKQNFGEDISNVDIFVVREN
                     SDRSGNTASIGGITKTNGSNYIDKVKDVNLIITKNIDSVTSTSTSSTYTINKEEISLK
                     ELDFKLRKHLIDKHNLYKTEPKDSKIRITMKDGGFYTFELNKKLQTHRMGDVIDGRNI
                     EKIEVNL"
     gene            1450..2958
                     /locus_tag="O0305_002480"
     CDS             1450..2958
                     /locus_tag="O0305_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCY0275586.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNIN
                     VDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTID
                     KKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEE
                     TSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKG
                     HTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRF
                     DDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLK
                     DVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPD
                     LQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKA
                     DREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(3061..3369)
                     /gene="spn"
                     /locus_tag="O0305_002481"
     CDS             complement(3061..3369)
                     /gene="spn"
                     /locus_tag="O0305_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCY0275587.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
BASE COUNT         1423 a          439 c          612 g         1093 t
ORIGIN      
        1 ttgcatataa ttctatgaga atgtggcaag gggctagtgg taaatcaaat tataatggga
       61 ttgttagccc tgcatatact gtgctttatc caacacaaaa tactagctca ttatttattg
      121 gatataagtt taaaacacat agaatgattc ataaatttaa aattaattca caaggattaa
      181 catcagatac atggaactta aaatataaac aattaaaaaa tataaatata gatatacctg
      241 tattggagga acaagaaaag ataggtgatt tctttaaaaa aatggatata ttgataagta
      301 aacagaaaat gaaaattgaa atattagaaa aagagaaaca atccttttta caaaaaatgt
      361 tcttataact ttgataaata catagattgc ataagaataa aatttgtata atttaacata
      421 aaagttgtaa aagtaaagtg aattaaaaac gaacattaaa tttaggcact gtgaaagcgc
      481 agtgtctttt ttgtgtcgaa attgtgtaca gaataagtag ttaaataaag attaagttga
      541 gataaagtgt tattcgtaaa taaaagagag tagatcgata ggaattgaat gatattagtt
      601 aactatttat taaattactt aataatgatt aatttttagt taaagtaagt ttaatgtgaa
      661 gcacgaccat tgctcattat aatgaatgag gattgttcgt attgcgtaat agaataaatc
      721 aaatagacta aaaattggga gcatagaatt atgaaattaa aaaatattgc taaagcaagt
      781 ttagcactag ggattttaac aacagggatg attacaacta ctgctcagcc agtaaaagca
      841 agtacattag aggttagatc acaagctact caagacttga gtgaatatta taatagaccg
      901 ttctttgagt atacaaatca gtcaggatat aaagaggaag gaaaagtgac gtttactcct
      961 aattatcaac ttatagatgt aactttaact gggaatgaaa agcaaaattt tggtgaagat
     1021 atttctaatg tagatatatt tgttgtaaga gaaaattctg atagatctgg taatacagct
     1081 tcaattggtg gtattactaa aacaaacggt tcaaattata ttgataaagt aaaagatgta
     1141 aatttaataa ttactaaaaa catcgatagt gttacatcaa cgtcaacatc atctacatat
     1201 acaattaata aagaagaaat ttcattaaaa gaacttgatt ttaaattaag aaagcattta
     1261 attgataaac ataaccttta taagacagaa cctaaagaca gtaaaattcg aattactatg
     1321 aaagatggtg ggttctacac atttgaattg aataaaaagt tacaaacaca ccgtatgggt
     1381 gatgttattg atggcagaaa tatagaaaaa attgaagtga atttataaaa ttattcgagg
     1441 gagcatatca tgagggaaaa ttttaagtta cgtaaaatga aagtcggttt agtatctgtt
     1501 gcaattacaa tgttatatat tatgacaaac ggacaagcag aagcatctga aaatcaaaac
     1561 gctttaatct ctaatataaa tgtagacaat caggaaaaac agaataatgt aaatcaagct
     1621 gttcagcctc aaaataatac taatgaaaca tcaaaagtac cggctaattt tgtcaaattg
     1681 aatgatatta aaccaggtga tacttctata caaggaacaa ctttaccaaa tcaatttata
     1741 ctattaacta ttgataaaaa agatgtgagc tcagttgaag attctgacag cagctttgtt
     1801 atgtctgata aagatgggaa ttttaagtat gacttaaatg gtcgcaaaat tgttcataat
     1861 caagaaattg aagtgtcttc atcagatccc tatttaggtg acgatgaaga agatgaagaa
     1921 gtagaagaaa cttcaactga agaagttggt gctgaggaag aaagtacaga agctaaagct
     1981 acatatacaa caccgcgata tgaaaaagcg tatgaaatac cgaaagaaca gctaaaagaa
     2041 aaagatggac atcaccaagt ttttatcgaa cctattactg aaggttcagg tattattaaa
     2101 ggccatacct ctgtaaaagg taaagttgct ctatctatta ataataaatt tattaacttt
     2161 gagacaaatg ctaatggtgg tccaaataaa gaagaagcga aatctggatc agaaggaatc
     2221 tggatgccta ttgatgacaa aggatacttt aattttgact tcaaaacgaa acgtttcgat
     2281 gatttagagt taaagaaaaa tgatgagatc tcattaacat ttgcacctga tgacgaagat
     2341 gaggcattga agtcattaat tttcaaaact aaggtaacga gtttagaaga tattgataaa
     2401 gcagaaacta aatatgacca tactaaagtg gaaaaagtaa aagtattgaa agatgttaaa
     2461 gaagatttac atgtagatga aatttacgga agcttatatc atacagaaaa aggtaaaggt
     2521 attcttgata aagaaggtac taaagtaatt aaaggtaaga ctaaattcgc aaatgcagtt
     2581 gtgaaggtag actctgaact aggtgaaggt caagaattcc ctgatttgca agtcgatgaa
     2641 aaaggtgaat tcagctttga tgtagatcat gctggattta gattacaaaa tggagaaaca
     2701 ctaaacttca cagtagttga tcctattaca ggtgaattat taagtggaaa ttttgtttct
     2761 aaaaacatag atatttatga atctcctgaa gaaaaagcag atcgtgagtt tgatgaaaga
     2821 atggaaaaca cacctgcata tcataaatta catggtgata aaattgtcgg ctacgatact
     2881 aacggattcc cgattacctg gttttatcca ttaggtgaaa agaaagttga acgtaaggca
     2941 ccaaaattag aaaaataatt aaataaaaca gcttaatgat gtaatgaaat tagtgagtta
     3001 atcactgact tctacgtcat tgagctgttt tttgtgcttt gttacaaagc attattgaat
     3061 ttattttacg tgttcatatt ttgaaacatc aaagccgtct tgtttagctt tgttgataat
     3121 gtctttgatt gaatgtagtc ctttatcggc gaagtatgat cttaagttgt cttttgtagc
     3181 ttggtcagca ttcttatcta ataacacatc aatataactt aattcatgtt ctaagaagtt
     3241 tgcatcatca tgtagtacga gtccattttg agaataaact ttcgcatctg cttgattacc
     3301 atatccaaca acgccagttg ctaaaacacc taccattgcc gtagctacta aaaccttttt
     3361 aaatttcata tctatcactc ctctaaaaat tgtaactcca tcataacact gaatattaag
     3421 aaaattacgt ttattaagtc gatttaataa tttttaataa atagttaaag tgacaaatat
     3481 tgtttaaatg caattaatct ttaatacgat gcttgaggat ttttcctaat aaaaccttga
     3541 tttcaaaaag ggtttaaatc aaatgaa
//
LOCUS       DAKTIP010000220         3565 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.223, whole genome shotgun sequence.
ACCESSION   DAKTIP010000220 DAKTIP010000000
VERSION     DAKTIP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.223"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(149..595)
                     /gene="perR"
                     /locus_tag="O0305_002482"
     CDS             complement(149..595)
                     /gene="perR"
                     /locus_tag="O0305_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCY0275588.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(692..1642)
                     /locus_tag="O0305_002483"
     CDS             complement(692..1642)
                     /locus_tag="O0305_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCY0275589.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEALLIEVLKSKVIRHAYLDVFENEPLKPNHELYELDNVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1648..2103)
                     /gene="bcp"
                     /locus_tag="O0305_002484"
     CDS             complement(1648..2103)
                     /gene="bcp"
                     /locus_tag="O0305_002484"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCY0275590.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2184..3473
                     /locus_tag="O0305_002485"
     CDS             2184..3473
                     /locus_tag="O0305_002485"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCY0275591.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
BASE COUNT         1150 a          599 c          527 g         1289 t
ORIGIN      
        1 aatattcttt tcttcaattt aaatattttt cagaataagc ataaaaaaaa gttaaacaac
       61 tttcatgtcg tttaacttct taaatattac gcttacttat atgaagtaag ctaacgcttt
      121 tttaatttgt catactacca aagttaaatt atttatcttg gcattcttta caaactccat
      181 aaatttccat tcgatgatgt gttacgtcaa agtcagtcat atgctgagct aatctttcaa
      241 tttcatttaa ctgtggatat tgaaaatcaa caatcttacc acattgttca catataatat
      301 gataatgatt atgtgtatta aagtcgaatc gacttgatga gtctccatat gttaattctt
      361 ttacaattcc aatatcttta aacactctta agttattata tattgtcgca acacttatat
      421 ttggaaaatc aggtgaaagt gcttgataaa tttcatcagc tgttggatga gtatgtgaag
      481 aaattaaata acgtaatatt gcttgtcttt gaggtgtaat tcttacgcct gcttgtcgca
      541 atgatgcaat tgattcttct agttcatgtt caattgattc tatttcaaca ctcatctata
      601 tcaccatctt tcttatataa taattattat tacttactat aaatataact gtagtattgc
      661 gcgcgtgtca atatttacat gatgatttca tttaatagcc ttttttagca tcaacttcat
      721 tctcaattag accattctta ttgagaaaat taactagatt gtttttaaaa atatctaata
      781 agtcatactt tgcttcataa tcattaccag ttatatgcgc tgttatagtt acattatcca
      841 attcatataa ttcatgatta ggtttcaaag gttcattttc aaacacatct aaatatgcat
      901 gtcgaataac tttacttttt aatacttcta ttaagagcgc ttctttaact atgctacctc
      961 gtcctatatt tataaaaagt gcttcatctt tcattaattc aaaatgtttt ttctttagta
     1021 aatgaatcgt ttcttgcgtt tctggtaaag catttataat aatgtcagca tttggtaatg
     1081 tgctttcaag tgattcgata gtatatatct cgtcaaactc atctttgttc tgacctgact
     1141 tgctcagacc aattaaattc atattaaaag cctttgctaa cttcgcagtt ctagtagcaa
     1201 ttgcacctgt acctaaaaat aaaactgttt gtcctgatag gcgtttacca gttattttcg
     1261 aatcatatat atgttgtcgt tggttatcat atgatagttt catcttttta taatcatcta
     1321 aaatgaaagc taaaatgtat tcagataatt gtttagcttg aacacctttt ccattagtta
     1381 aaagtatgcc gtgatctgca atataatcta acggcaatgt atttacaccc gttgcaaacc
     1441 atgcaatcca ttttaaattc gggcaacgtc gtaaaaatgc ctcattgata ccgccatcat
     1501 aaccaactaa tatatctaaa tcagccaagt cgctctcagg tatttctgat gcttttttat
     1561 aaaatttaaa atctaaatct gaaaagcgtt gttgtaattc agtttcaact tcacgcatac
     1621 gatttaaccc aacaactttc atatatgtca ccccaaaatg tttttaagtt cttctatttg
     1681 tgttttaacc ttaaccttct cgataacatc taatacttta ccttgttcat ctattataaa
     1741 agtcgttctt acaatgccca tactttcttt gccaaatgat ttttttaact gatatacgcc
     1801 agtttcttta gctaatttaa aatcttcatc tactaataaa tcgaaattca atccgtgttt
     1861 ctcaataaaa ttttggtgtt ttttctttga atcaccgctt ataccatata ctgcaacatc
     1921 taaatcattg aacatttcta aattgtctct aaagtcacaa gcttctgtgg tacaagtagg
     1981 tgtattatct ctaggataaa aatatataat cgccttttta ccttttaatg tatcatttgt
     2041 aatgacagtt ccgtcttgat tttctaattt aaatattgga aattgttctc ctttttgcaa
     2101 catataatca cctttttctt cattataata attttatgat acgatattag atgaaaatat
     2161 taaattaaaa gaggttgata taaatgaatt ttagtgaaag tgaacgttta caacaacttt
     2221 caaacgaata tattctaggc ggtgtcaatt ccccttctcg ttcttataaa gctgtaggag
     2281 gcggtgcacc tgttgttatg aaagaaggac acggtgcata tttatatgat gtcgatggca
     2341 ataaatttat tgattacctt caagcatacg gtccaattat tacggggcat gcacatcctc
     2401 atattactaa agcaattcaa gaacaagctg ctaaaggtgt tttatttggt acaccgactg
     2461 aattagaaat tgaattcagc aaaaaattac gtgatgcaat tccatctctt gagaaaattc
     2521 gctttgtaaa ttctggaaca gaagcagtca tgacaacaat tcgtgttgca cgtgcatata
     2581 ctaaaagaaa taaaattata aaatttgctg gatcttatca tggccattct gatttagtat
     2641 tggttgcagc aggtagcggc ccatctcagc tcggttctcc agactcagct ggtgttccag
     2701 aaagcgtcgc acgtgaagtc attactgtac ctttcaatga tattaacgcc tataaagaag
     2761 caattgaatt ttggggtgat gaaattgccg cagtattagt agaaccaatt gttggtaact
     2821 ttggaatggt aatgcctcaa cctggatttt tagaagaggt taatgaaatt tcacataaca
     2881 atgggaccct agtgatttat gatgaagtaa ttactgcatt ccgtttccat tacggtgccg
     2941 ctcaagattt attaggtgtt atccctgatt taactgcatt tggtaaaatt gttggcggtg
     3001 gtttaccaat tggaggctat ggtggacgtc aagatattat ggaacaagta gcacctctag
     3061 gacctgcata tcaagctggt acaatggctg gtaacccgtt atctatgaaa gcaggtattg
     3121 cattactcga agtactagaa caagacggtg tttatgaaaa attagacagc ttaggccaac
     3181 aactagaaga aggtttactt aaattaatcg aaaaacataa tatcacagct acaattaatc
     3241 gtatttatgg atctttaaca ttgtacttta cagatgaaaa agtcacacat tatgatcaag
     3301 ttgaacattc tgacggcgaa gcgttcggta aatttttcaa attaatgtta aatcaaggta
     3361 tcaatttagc accttctaag tttgaagctt ggttcttaac aactgaacat acagaagaag
     3421 atattaaaca aactttaaaa gctgcagact atgcttttag tcaaatgaaa taacttttaa
     3481 ggaagtatat ttttaattga attaaaataa agcaaatgct cacatagtag attcatttta
     3541 tatcgttaac tattaacgtt taact
//
LOCUS       DAKTIP010000221         3532 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.224, whole genome shotgun sequence.
ACCESSION   DAKTIP010000221 DAKTIP010000000
VERSION     DAKTIP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.224"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..1427
                     /locus_tag="O0305_002486"
                     /pseudo
     CDS             1..1427
                     /locus_tag="O0305_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MAP domain-containing protein"
     gene            complement(1751..3037)
                     /locus_tag="O0305_002487"
     CDS             complement(1751..3037)
                     /locus_tag="O0305_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0275592.1"
                     /translation="MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            3237..3335
                     /locus_tag="O0305_002488"
     CDS             3237..3335
                     /locus_tag="O0305_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275593.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT         1314 a          489 c          569 g         1160 t
ORIGIN      
        1 tttaagatta tcgaagcaag cgaaatatac agttaatttt aaaaatggaa cgaagaaagt
       61 tatcgatttg aaatcaggta tttacacagc gaatttaata aattcaagtg atattaaaag
      121 tatcaatatt aacgtagata caaaaaaaca tatcgaaaat aaagctaaaa gaaactatca
      181 agttccatat tcaattaatt taaatggtac atctacaaac attttatcga atctttcatt
      241 ttcaaataaa ccttggacaa attacaaaaa tttaactagt caaataaaat cagtactgaa
      301 gcatgataga ggtattagtg aacaagattt aaaatatgct aagaaagctt attatactgt
      361 ttattttaaa aatggtggta aaagaatctt acagttgaat tcaaaaaatt acacagcaaa
      421 cttagttcat gcgaaagatg ttaagagaat tgaaattact gttaaaacag gaactaaagc
      481 gaaagcagac agatatgtac catacacaat tgcagtaaat ggcacatcaa caccaatttt
      541 atcagattta aaatttacag gtgacccacg tgtaggctac aaagatatct ctaaaaaagt
      601 taaatcagta ttgaagcatg atagaggtat cggggaacgt gaattaaaat atgcaaaaaa
      661 agcaacttac acagtacatt ttaaaaatgg aacgaaaaaa gtgattaaca taaattcaaa
      721 tattagccaa ctgaatctgc tttatgttca agatattaaa aagatagata ttgatgttaa
      781 aacaggaact aaagcgaaag cggatagcta tgtaccatat acaattgcag taaatggcac
      841 atcaacacca attttatcaa aacttaaaat ttcgaataaa caattaatta gttacaaata
      901 tttaaatgac aaagtgaaat ctgtattaaa aagtgaaaga ggcatcagtg atcttgactt
      961 aaaatttgcg aaacaagcaa aatatacagt atatttcaaa aatggaaaga aacaagtagt
     1021 gaatttaaaa tcagacatct ttacacctaa tttatttagt gccaaagata ttaaaaagat
     1081 tgatattgat gtaaaacaat acactaaatc aaaaaaaaat aaataaatct aataatgtga
     1141 aattcccagt aacaataaat aaatttgaaa acatagtttc aaatgaattt gtgttctata
     1201 atgcaagcaa aattacaatt aatgatttaa gtataaaact taaatcagca atggcaaatg
     1261 atcaagggat aactaaacat gacataggac ttgctgaacg cgcagtgtat aaagtgtatt
     1321 ttaaaaatgg ttcgtcaaaa tatgtagact taaaaactga gtataaagat gaaagagtat
     1381 ttaaagcaac tgacattaaa aaggtagata ttgaacttaa attctaatct ttaattttat
     1441 aataaggcat ctcacaatcg tggggtgcct tttacatttg tagagatgtg atgcttgaat
     1501 gtttgaagtg atttgccaaa agttatataa attggttcta taataaatcg gactgataga
     1561 aaaagttttt tacttttcat ctgtccgatt ttttgatttt gttaggagag atgtataaaa
     1621 ctaattaggt atagtgtttg acaagttgct gaataaaata tatcttatga tactttgaaa
     1681 gaaggaataa ttttaaaaat aaaaaaccaa taatccgagt catactcatc agattattgg
     1741 ttgaaattaa ttatcttaag tcatcaatcg ctttagcaat tgaatcaaat acatgtggga
     1801 tatcttcttt ttctacacag ctaaacgcaa tacgaatatc tgttgcatta agtgcaataa
     1861 caccaattga atatttttca attaaatgtt tacgaagttc ttcagggtca acatcatgaa
     1921 cttttattgc cataaagtat ccagagttaa agtcataggc ttgccaatgt gaatgatatt
     1981 gatcagcata gacaacttcc ttagtgactt catagcgttc ttttaatgtt tgaatatttt
     2041 gttcgatttc tttatcgaat tgtttattat tttttaatac atgcttaaca gcgctttgtg
     2101 ttggaagagg tccactagaa atgttacttc gtataagacc ttttactttg gcttctaata
     2161 cttctttagt tgtttgatca gacgttccaa atgtcataaa cccaacacgg aatccccatg
     2221 cgaaaaattc ttttgttgca ccatctaaac gaacaggtaa tattgcattt gaatttaaat
     2281 tagataatgc agtaaataat gattgagtat acacatcttc atagaataaa ccatagtatg
     2341 cgtcatccac aacagctata acttttgtac ctttatcagc taatgcttta attgcatcga
     2401 cgatggtagt cacttcttta tgcgtaggtg tgtaacctgt cggattatta ggataattta
     2461 aaatcataat gactttatct ttattgtatg attgtaaagc ttctacaagt gaatcagtag
     2521 tataatgccc gtctttatca aagataggat atgtttgaag gtttgcacca tttctcgtat
     2581 tgaaaacaag tttgtaatta ccccaattat gctctggtaa caagatagtg tcaccttgat
     2641 ttacaaataa atcgccaact aaagataaac catgtgttaa tgcattcgta acaattggta
     2701 gtgacatgtt gtcgattgat agctctggat tgtcacgcaa cattttttgt tgccataaat
     2761 cacgtaattc ttcgatgcct tgtggtggcg cataagggaa tatttcatct ggagttaaat
     2821 cattaaacat tgcatctaaa gatgatgcaa acatttttcc gtctttgttt gtcgccatac
     2881 ctatagttgc attatatgtt gtactctttg cttcagcaga ttgagataaa atgccttttg
     2941 gataaaacat attttgacct aagtcagata acattgtaaa ggcagttgca tttgactgtt
     3001 gaagttgttc atttaagctt tgggctaaag gattcattat tgcttaacct ctctttaaaa
     3061 aaattgtatg cctatatatt atcttcaatt ataccaaaga taaagtgttg ttggtgaatt
     3121 aacgaataat atgtaaaatc tgctgccaaa atacgaacac atgttctgtt tttatgttag
     3181 aataggtgta ggtcatcctt cagtatgata ctttagtcga acggaggtgt agtgtaatga
     3241 tgtggtggca agaagggatg atgacactta ttacaggtgg cttactaatt atttttcgac
     3301 tttggttaga actgaaatgg aaaaataaaa aatgatccta cacacataaa taaccccaag
     3361 tctgctggta ccagacttgg ggttgtgtag tataattctg tggatacact taaataatat
     3421 cattgaacac aatatatcgc aatcaataat cattttgatt gaatggtttt gttgcgctat
     3481 tttagttgaa agtgattagt accttgaact tttgaaattc aataacttat ta
//
LOCUS       DAKTIP010000222         3485 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.225, whole genome shotgun sequence.
ACCESSION   DAKTIP010000222 DAKTIP010000000
VERSION     DAKTIP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.225"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(240..920)
                     /locus_tag="O0305_002489"
     CDS             complement(240..920)
                     /locus_tag="O0305_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCY0275594.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSESELKH
                     YYNKPILERKNVTGFKYTDEGKHYLEVTVGQQHSRITLLGSDKDKFKDGENSNIDVFI
                     LREGDSRQATNYSIGGVTKSNSVQYIDYINTPILEIKKDNEDVLKDFYYISKEDISLK
                     ELDYRLRERAIKQHGLYSNGLKQGQITITMNDGTTHTIDLSQKLEKERMGESIDGTKI
                     NKILVEMK"
     gene            1392..2237
                     /locus_tag="O0305_002490"
     CDS             1392..2237
                     /locus_tag="O0305_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0275595.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVPVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            2256..2615
                     /locus_tag="O0305_002491"
     CDS             2256..2615
                     /locus_tag="O0305_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCY0275596.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            3139..3291
                     /locus_tag="O0305_002492"
     CDS             3139..3291
                     /locus_tag="O0305_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275597.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
BASE COUNT         1172 a          579 c          531 g         1203 t
ORIGIN      
        1 tttgaatcat acacccatat tataggagct gtattcggat attcacatta acctgttttt
       61 aactattcat aaaatatgat taagctattt aagcaaaaga taatgcgtgg tgcgactgtt
      121 tgtgtgagtt ttgtaacttt tcccctcaaa aaaagaagct aagcaacagt acgtcgctta
      181 acttccatat atttccataa caagcactat tcaacatagc gctttgttgt tagaaagtat
      241 tatttcattt ctactagaat tttattaatc ttagtgccgt cgattgactc acccatacgt
      301 tctttttcaa gtttttgact taaatcgatt gtatgtgttg tgccatcatt cattgtaatt
      361 gtaatttgac cttgtttaag accatttgaa tacaagccgt gttgtttaat cgcacgttct
      421 cttaatctat aatcaagttc ttttaatgag atgtcttctt ttgaaatgta gtaaaaatct
      481 ttaagtacat cttcattatc tttcttgatt tctaaaattg gcgtattgat ataatcaata
      541 tactgcacac tatttgattt tgtaacgcca ccaattgagt aatttgttgc ttgtctactg
      601 tcaccttctc taaggataaa cacatctata tttgagtttt ctccgtcttt aaatttatct
      661 ttatcagatc caagtaaagt gattcgagaa tgctgttgcc ctactgtgac ttctaaatag
      721 tgtttaccct catcagtata tttaaatcca gtcacatttt tacgctctaa aattggttta
      781 ttataatagt gttttaactc tgattcactt tgttgtttaa cttctgcttt cgcttgtact
      841 gattgtacat tcgatgtaat tacacctgtt gctaacattc ccaatgctaa acttgctttt
      901 gctatcgctt taaatttcat aatttttagc cccaatctat ttaaatttga tttttcaatt
      961 acgtaatatg aacaatctac ctacattata ttgaggcgct atcccctttc aaattaaacc
     1021 tcatttaact aaacttgaat cattgttaag gtaattaata aaatgctaac tgttattaat
     1081 caattcttat ccgtcgagac tcttttttat acaaatcaca ctttatacca tttttatttt
     1141 aaaaactgta aaactttcag acacaaaaca gacatcatat tgtcgtagta cctgattaaa
     1201 attttcaatg atattagtga tacacatcaa ctaaaacgcg tcattacggc attttcaaca
     1261 ttaataatat ttacgtattt actatacacg tcgacaataa atacagatta gaatttttta
     1321 taaaatcaaa tgttttgaaa accatcaaat aagtattatg ttatgtatat aaagattagg
     1381 agatgaagtg aatgaatatt atgttaacag gtgctacagg tcatttaggc acacatatta
     1441 caaatcaagc cattgcaaat cacatagatc attttcacat tggtgttaga aatgttgaga
     1501 aagttccaga agattggcgc ggaaaagttc ctgttcgaca attagattat tttaatcaag
     1561 aaagcatggt agaagcattt aaaggaatgg ataccgttgt gtttattcca agtattatcc
     1621 atccatcatt caaacgtatt cctgaagtgg aaaatttagt atatgcggca aagcagagcg
     1681 gcgttgctca tatcattttc ataggttatt acgcagatca gcataataat ccattccata
     1741 tgagtcctta ttttggttat gcagcacgtc tattggcaac aagtggcatt gactatacgt
     1801 atgtaagaat ggcaatgtac atggatccac ttaaaccata tttaccagaa ttgatgaata
     1861 tgcataaact gatttatcca gctggcgatg gtcgtattaa ttatattact agaaatgata
     1921 ttgctagagg tgtcattgct attattaaaa atccagatac ttggggcaaa cgctacttat
     1981 tatcaggcta cagttatgat atgaaagaac ttgctgcaat tttatctgag gcatcaggca
     2041 cagaaattaa atatgagccc gtttcattag agacatttgc agaaatgtat gatgaaccta
     2101 aaggctttgg tgcattattg gcatcaatgt acgacgcagg agcaagagga ctattagacc
     2161 aagaatccaa tgatttccaa caattagtca atgatcaacc acagacactg caatcatttt
     2221 tacaagaaaa tatttaataa taaaggagcg ttatagtgaa tatcatctca acaattttaa
     2281 tcatatttgt ggcattagag tttttctata ttatgtacct tgaaacgatt gctacaactt
     2341 ccaaaaagac tagcgagaca tttaatataa gcgtcgataa attgaaagac aaaaatatta
     2401 acctactttt gaagaaccaa ggcgtatata acggtttaat cggagttttg ctaatatacg
     2461 gtttgtttat cagcagtaat ccaaaagaaa tatgcgcagc tattttagtg tatatcattg
     2521 gcgttgctat ttatggtggc ctttcaagca atattagtat ctttttcaaa caaggcacat
     2581 tgccagtatt ggcactcata tcaatgcttt ggtaagtatt ggtgtttggg ggggtggaga
     2641 tgtagtcgga ggtttggagg atttgagcga attgtgtgtg gactttagac tcagagtatt
     2701 tcatcctaat tatttcaagc agaggtgaca gtagcgttgc ctctgtttcc ttatagaaaa
     2761 attatttaat gaagaaaacc catatctgat ttaattttca gctgataaat actccatata
     2821 ttagaatggc tactttatct attgcatcaa tcctttaaaa caaaaaaccc atgatttcga
     2881 aattcccgta tgatggggtt cctactctca tggatcagtt aaataaatat tatcactatc
     2941 agtttattat ttcaatatta ttaacaatat atgtagtcgt aaaaggaaag aggacatgag
     3001 aacttcggtg ttgattggca ttacataacg cttccaaaca tattatttgg taacaataag
     3061 aaactattta cacaatatat tttgtatagt aaaattattt tataatattt aaatccaatt
     3121 gcacaaggag tgattatcat ggtaccagaa gaaaaaggtt ctattacttt gtcaaaagaa
     3181 gcagctatca tatttgcaat cgcaaaattc aaaccattta agaacagaat taaaaataac
     3241 ccacaaaaaa caaatccatt tcttaaatta catgaaaaca aaaaatctta atcactttat
     3301 ttatagcatt tctaatctca gaaatgctat aaaggacaac gccctctttc ataactatat
     3361 aagtcaagaa accaagacat ttcaaacatc tttaacccct tcaatagcac attccctaaa
     3421 acataaagca ataacaatga acgatcaaaa ttccttacgt acttatgatg tgtcagtgaa
     3481 tcgtc
//
LOCUS       DAKTIP010000223         3479 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.226, whole genome shotgun sequence.
ACCESSION   DAKTIP010000223 DAKTIP010000000
VERSION     DAKTIP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.226"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(12..1622)
                     /locus_tag="O0305_002493"
     CDS             complement(12..1622)
                     /locus_tag="O0305_002493"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCY0275598.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(1958..2488)
                     /locus_tag="O0305_002494"
     CDS             complement(1958..2488)
                     /locus_tag="O0305_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCY0275599.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(2600..3460)
                     /locus_tag="O0305_002495"
     CDS             complement(2600..3460)
                     /locus_tag="O0305_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275600.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
BASE COUNT         1082 a          680 c          455 g         1262 t
ORIGIN      
        1 tattagcaaa tttatttatt ttgttgatat tttaatgaag cttcaataaa tgatttaaaa
       61 atcgggtgcg gacgatttgg tctagataag aattctgggt ggaattgaca agcaataaag
      121 aaatcatttg tcggaatctc taccatttct actaaacgtc catctggact tgtaccagaa
      181 atcaccatac catttgcttc taattgttct ctatagtcat tattaaattc ataacgatga
      241 cgatgtcttt cttcaatttc cgctttacca taaacatctt gtgccaatgt gccttcttta
      301 attgaacatg gatataagcc taagcgtaat gtaccaccta aatcttcgat atctttttgt
      361 tctggtaata aatctataat tgggtatggt gttgctgggt ctaattcagc tgaatgtgcg
      421 ccttcaaggc ctaatacgtt tcttgaaaat tcaactgttg caagttgcat tcctaaacaa
      481 ataccaaaga atggtacatt gttttctcta gcatacttaa ttgcactaat tttaccttca
      541 cttgcacgga aaccaaatcc acctggtact aaaataccgt cgacatctgc aaggtattcg
      601 gctgcatttt catctgttac ttcacttgaa tcaatccatc taatgtcaat atctttggca
      661 aaaggatatc cagcatgttt caatgattca acaactgata aatatgcatc ttgtaagcta
      721 acatatttac ctactaaacc aattgtaatt ttaccatcta aattattaac gatatctaac
      781 aactgtttcc attcatcaag ctgtgtttca tatttcgcgt ttaattgtaa acgtttaata
      841 acgatatcat ccatattttg ttggcttaat tgtaatggaa tttcgtataa agagtctgca
      901 tcacgacatt caataacact ttctttatta atgtcacaga ataatgcaat tttatctttt
      961 aaatcttgtg tcatttcata ttcagttctt acaacgatta agtctggttg aatacctaag
     1021 cctcgtaatt ctttaacact atgttgtgtt ggcttcgttt tcatttctcc agcagcttta
     1081 atataaggca gtaatgtaca gtgaacatac ataacatttt ctctacctaa atcgctacga
     1141 atttgacgaa tcgcttcaat aaacggtaat gactcaatat cacctgttgt accgccaatt
     1201 tcagtgataa caacgtctgc attcgtactt tcccctgcaa gtaataaacg ttctttaatt
     1261 tcatttgtaa tatgcggaat aacttgaact gttccgccta agtaatcacc acgacgttct
     1321 tttttcaata cgtgtgaata cactttaccg gctgtcacat ttgaaaactt gtttaaatta
     1381 atatcaataa atctttcgta atgtcctaag tctaggtcag tttctgcacc atcatccgtt
     1441 acgaatactt caccatgttg ataaggactc attgtacctg ggtcaacatt taagtatgga
     1501 tcgaattttt gaattgttac atttagacct ctatctttta ataatctacc tagagaagat
     1561 gctgtaatac ccttccctaa tgatgaaact acgccacctg ttacaaaaat aaattttgtc
     1621 atgtcttgac ctcctaatta ttaaagggaa tgcttcctca tcaattgtaa aaattacgta
     1681 aaacataaca ttaaagcaag ctcattcaag ttgtttattt gtcgaattcg acaaaattaa
     1741 attctgttta cttaccgttt ccatactgct atgtcactat gtatatcatt tacaactttg
     1801 atttttgcgt tatcatttct tgatttactc aattaaataa aaaaacgctc cctctcacaa
     1861 attctgtaag gggagcgtat aaattttata cacgtgtcct atttaaagga gcccgaataa
     1921 aattttatca tctttcaact aaaagtcaaa caaaaaatta atcgttgaag tcttcttcgt
     1981 cttcaaacac ttcttcgtct tcgtctagtt catcttcatc ttcttcaatg actatatccg
     2041 aatgattgat ttcttcttca acctgctcat cttctggatc atttagttct tcttgatcat
     2101 ctgtttgagc tggaatatca tcgtcgtcat ccatttcatc ttcgcccaat aattttaagt
     2161 tttgatcttc ttcatcatct gcatccagaa tatcgaattt ttgaatagtt ggtgcgattt
     2221 tctcttcaat atcatctacc gaataccaat cacgtaatcc ccataaattt tctccaacat
     2281 ttaaaaaacg accatctgtg tttaaatccg tgtaaaattg tacaacacga ttttcaattt
     2341 cttcgtactc ataatcacct aacgctctaa attcatcgat gatatcatat aagttcattg
     2401 tttcgccttt atcattcaat aatgtataag ccatatcaat aaatgatttt tcatcaacca
     2461 tttgttttgt ataatcttga attttcattg cccaatactt cctttcgaag gatataatct
     2521 aaatttatac gtctgaattc atttatatat agtaacaaaa ctaaattata aatgacaata
     2581 tcatatttcg caatcactgt tagtaattta aatatttttc gaaaacgaca taatcatcag
     2641 tttgttcgat ttcaacgaaa ttactattta aacataatgg tttaaaatca ttattctctc
     2701 caaacagaat catcgtaatc acattaaggt tgtcatagtt ttttcttaag taagttggca
     2761 attgtcgcag agcacttgaa ccaataccat taaagcgata tgatttatcc acttcaatag
     2821 cagcaatgtt catgcgttca ccaatctgct ccaatgcgat aaagccaacc ttttggccat
     2881 caatcattaa attaatgata tgtacattgg tcacttctga acgactttct tcttcaagct
     2941 ttaatgatga aacatgtttt gaatctaagt ccaagtaata tagtcgttct ttgccaattg
     3001 gtccttcttc tttagtcgct gtatgcatga atccagcacg ttcgtatagg ttccaagcat
     3061 tgtcattttc cgcatctact actagatata gatgattaaa atcaggaaat acaccttgaa
     3121 catattgcgg caatgacatc attattttcg tgccatatcc aaaaccttga tatttttcat
     3181 taatcgataa tgaacgaata taaacgacat tttcaggtgt atcataacct tcatgctgat
     3241 agtaacgatg taatacaaaa aagcccacca cttcattttt atcgtttatt gctacgttag
     3301 caatcctatc agcatcatta attgcatcat caataacttc tttaggtaaa gatgaatata
     3361 tttgttgtct ttcattcaag tcaaaatcat taattgctga acgatattgt tcatcaaatg
     3421 cttttaaagt gataccttca aaacaaagtt ctttagccat atatttaacc cctatcttt
//
LOCUS       DAKTIP010000224         3466 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.227, whole genome shotgun sequence.
ACCESSION   DAKTIP010000224 DAKTIP010000000
VERSION     DAKTIP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.227"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            116..1375
                     /locus_tag="O0305_002496"
     CDS             116..1375
                     /locus_tag="O0305_002496"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCY0275601.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTKLEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1464..1799
                     /locus_tag="O0305_002497"
     CDS             1464..1799
                     /locus_tag="O0305_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275602.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            2037..3464
                     /locus_tag="O0305_002498"
     CDS             2037..3464
                     /locus_tag="O0305_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCY0275603.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
BASE COUNT         1257 a          465 c          712 g         1032 t
ORIGIN      
        1 tagaaagaca agattttaac gattaaaata aactatttta caaataaagt aaaattaatt
       61 tataatgcta ataatgcaaa aaattaaaaa gtaatggaca aaggagataa atgatatggc
      121 tcaagaggta ataaaaataa gaggtggacg cacactaaat ggggaagtta atatcagtgg
      181 cgcgaaaaac agtgctgtag ctatcattcc tgcaacatta ttagctcaag gacatgtgaa
      241 attagaaggg ttaccgcaaa tctctgatgt taaaacttta gtgagtttat tagaagattt
      301 aaatattaag gcatcactaa atggtacgaa attagaagtt gatacaactg aaatacaaaa
      361 tgctgcatta ccaaataata aagttgaatc attacgtgct tcatactata tgatgggtgc
      421 catgttaggt agatttaaaa aatgtgtgat tggtttaccg ggaggatgtc cacttgggcc
      481 acgtccaatt gatcaacata ttaaaggatt taaagcttta ggtgctgaaa ttgatgaatc
      541 aagtacgaca tcaatgaaaa tcgaagctaa agagttaaaa ggtgcacata tttttctaga
      601 tatggttagc gtaggtgcaa caattaacat tatgctagct gcagtttatg caacaggaca
      661 aactgtaatt gaaaatgctg ctaaagaacc ggaagtagtt gatgttgcta acttcttaac
      721 aagtatggga gctaatatta agggagcggg tacatcaaca attaaaatca atggcgtcaa
      781 agaattacat ggttctgagt atcaagttat acctgataga attgaagcag gcacatatat
      841 gtgtatcgct gcagcatgtg gagaaaatgt tatattaaat aatattgttc cgaaacatgt
      901 agaaacatta actgctaaat ttagtgaatt aggtgtgaat gttgatgtaa gagacgaaag
      961 aattcgcatc aataataatg cgccatatca atttgttgat attaaaacac tagtatatcc
     1021 aggttttgct actgatttgc aacagcctat tacaccatta ttatttatgg caaatggtcc
     1081 ttcatttgta actgatacga tttatccgga gcgttttaaa catgttgaag aattaaagcg
     1141 catgggtgca aatattgaag ttgacgaagg cacagcaacg ataaaaccat caacattaca
     1201 tggtgctgaa gtttatgcta gcgatttaag agcaggagct tgtttaatta ttgctggttt
     1261 aattgctgag ggtgtaacta caatttacaa tgttaaacat atttatagag gttatacaga
     1321 tattgtagaa cacttaaaag ctttaggtgc agatatttgg acggaaactg tatagattta
     1381 tcttgttttg tggtataata attatactga ttaaaaaatg gtgttcgttt acattctaaa
     1441 tttattgatg agaggtgaag atgatggaag tatgtccgta tctcgaagaa acttttaaaa
     1501 tacttggtag aagttggaat ggattaatca ttaattatct ctcaagatgt aatgactgtt
     1561 cagcacactt ttccgatatg aaaagagatt tgaaaacaat aacaccacgt gctttaagtc
     1621 ttaagttgtc agagcttgca caatgggagt tagttgagaa gcaaatcatt tctacgagtc
     1681 cagtacaaat tatttatgtg ctaactgaaa aaggtaaagc gttagcagag gctttacatc
     1741 caattgaagc atgggcgcaa tcatatgtcg atttaacaga tcaacgtact gctaaataat
     1801 tacatgacgt ttaaacatag cattgattat aactatttct aagtcttcgc attatttgcg
     1861 atgatgtggg aatagttatt tttatttaaa aatataaaaa aatagatgca gcaaaatttt
     1921 aaagcatttt attttgaaca tattaaaagg gagcgtatca taatggaatg taatgtttat
     1981 atcgtatgca ttacggataa ataatatata aatcattctt gaggagtgaa agaataatga
     2041 gagactacac aaagcaatac attaatggcg aatgggtaga aagtaatagt aatgaaacga
     2101 tagaagttat aaatccagca accgaagaag taatcgggaa agttgctaaa ggtaataaag
     2161 ctgatgttga taaagccgtc gaggcggcag acgatgttta tttagagttc cgtcatacat
     2221 ctgtgaaaga aagacaagcg ttattagata aaattgtaaa agaatatgaa aacagaaaag
     2281 acgatattgt acaagctatt acggatgaat taggtgctcc tttatcatta tctgagcgtg
     2341 tccattatca aatgggacta aaccattttg ttgcagcgag agacgcatta gataactacg
     2401 aatttgaaga acgccgcgga gatgatttag ttgttaaaga agcaatcggt gtatctggat
     2461 taattacacc gtggaacttc cctacaaacc aaacatcatt aaaattagca gcagcatttg
     2521 cggctggtag tccagttgta cttaaaccat ctgaagaaac accatttgca gctgttattt
     2581 tagctgagat ttttgataaa gtcggtgttc ctaaaggtgt atttaacctt gttaatggtg
     2641 atggtgctgg tgttgggaat cctttatctg aacatcctaa agtacgcatg atgtcattta
     2701 caggatcagg ccctactggt tctaaaatta tggaaaaagc cgctaaagat tttaaaaagg
     2761 tatcattaga gcttggtggc aaatcaccat atatcgtcct agatgacgta gatattaaag
     2821 aagcggctaa agcaacaaca ggcaaagttg ttaataatac tggtcaagta tgtacagctg
     2881 gtacacgtgt tttagtgcct aacaaaatta aagatgcatt cttagctgaa ttaaaagaac
     2941 aatttagcca agtgcgtgtc ggtaatccaa gagaagatgg tacacaagta ggccctatca
     3001 ttagtaaaaa acaatttgat caagtacaaa attatattaa taaaggtatt gaagaaggtg
     3061 ctgaattatt ttatggtggt cctggtaaac cagaaggact tgaaaaagga tactttgcac
     3121 gtccgacaat ttttattaat gtagataatc aaatgacgat agcacaagaa gaaatttttg
     3181 ggccagtaat gtcagttatc acttataacg atttagatga agcgattcaa attgcaaatg
     3241 atacaaaata tggtttggca ggatatgtta ttggtaagga caaagaaaca ttgcataaag
     3301 tagctcgttc tattgaagca ggtacagtag aaataaacga agcaggtaga aagccagatt
     3361 taccatttgg tggctataaa caatctggtt taggtcgtga atggggcgat tatggtattg
     3421 aagagttctt agaagtgaaa tctatagctg gatattttaa ataaat
//
LOCUS       DAKTIP010000225         3442 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.228, whole genome shotgun sequence.
ACCESSION   DAKTIP010000225 DAKTIP010000000
VERSION     DAKTIP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.228"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..446)
                     /locus_tag="O0305_002499"
     CDS             complement(<1..446)
                     /locus_tag="O0305_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCY0275604.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSG"
     gene            complement(506..1030)
                     /locus_tag="O0305_002500"
     CDS             complement(506..1030)
                     /locus_tag="O0305_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275605.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            1151..1714
                     /locus_tag="O0305_002501"
     CDS             1151..1714
                     /locus_tag="O0305_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCY0275606.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(1780..2520)
                     /gene="pmtD"
                     /locus_tag="O0305_002502"
     CDS             complement(1780..2520)
                     /gene="pmtD"
                     /locus_tag="O0305_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCY0275607.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(2520..3392)
                     /gene="pmtC"
                     /locus_tag="O0305_002503"
     CDS             complement(2520..3392)
                     /gene="pmtC"
                     /locus_tag="O0305_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCY0275608.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
BASE COUNT         1248 a          572 c          422 g         1200 t
ORIGIN      
        1 aatcctgaat atgatatggc aaaaaataca ttactaattg agtccacaat tcctctggat
       61 attgctgatg ctaataaaat aattggacaa aacagcagca aaagtgatat taaaaataat
      121 gctaatacaa ataatccaac tatagatact ggtataagga tgacaaaaat agatagaatg
      181 cctaaactta atgaagcagc aacagctctt gaaatatttt caaatgttgg tttagattca
      241 gcataatcaa tgacacttct agcttttgtt tcagctgcaa catcttgtgg gtcttttaac
      301 ttctttaaaa tttccccttc atcaacgcca ttaagttctt gctcataaaa ataacgttca
      361 tattcataca ttttttgatc tcttaaatgc ctaggtagcg catcaagtgc tgtttctaat
      421 tcatttaaat aagtaatctt gttcatatag gtcactcatc ccaataaaat tattcattcg
      481 ttttttataa ctttaagacc taaagctatt tactaaaaaa ttcaatacgt ttaatttcta
      541 aatcttgata aagtaagttt aattttaaat ggttatctct atcaatatac tttaccgatg
      601 agaaatcatt gccaactttt gttcttttat atccgaatcc aagattttca atcgcgttat
      661 ttatcggcga accaattccc ccatcaaaat tagtatgtat cttttcatct cttactaata
      721 ttatcccctt aacgatgtta tccttttctc tcactttaac ggtaagatca gggtgagctt
      781 tactgttata aaatttaaaa cgatcaaact taattttttt atttacttca tagccttttt
      841 tatcaaactt ctcatttaat ttaatatcgt ttatttcaag attagacaaa tccacagttt
      901 tcgtatgacg atattgatat aaaccaatta atacaccaaa agaacagatt aaaaataata
      961 taaacacaac ccagcgattt tgaatacgca ctgatgtcac cctttctttg tacttattta
     1021 aatatagcat aaggtgcttc ccgtttcatc cttaacaaaa attaacaaag aatttgcaat
     1081 taatttattt cattgccttt tattactata aattggtata atgttgtaaa tgctaaagga
     1141 ggtttttaca atgacaaatt tagaaactta tttcaaaaat agccaaccct taaatgaata
     1201 cattgatgga atgaagacaa atcaagatac cgttctcagt atctatcaat catttgagtt
     1261 accagaagat gattcacgca tcaaaaaaat aaaagagatg aattattcta aagtattagt
     1321 aattactgaa gactggtgtg gagatgcaat gatgaatctc ccaattctaa aacacatcag
     1381 tgaagcatta aatttagaag tacgcgtgtt ccatagagat gatgatacaa aactgatcga
     1441 tcaatattta acaaatggta aatcacgtgc aattcctatc ttcgtattct tgaatgatca
     1501 attcgaacaa gaaacagttt ggggaccaag agcaaatgaa gtccaaaaat ttgtaactga
     1561 tgttcgtgct gataaattac cgagcaaaga tcatccagac tatgcagatt tagaaaaaga
     1621 aacacactta gttatatcaa accgctataa aactgacgct tcattttgga aagcagttta
     1681 taattccatc ctaaataaat tagaaaataa ataaatttac aataagacaa actttgaggt
     1741 tgggaaaatt taaaattccc aacctcaaaa ttatttttat taaatgtctt ttttagcgac
     1801 atataagata ttcaaaatga agaatatcac tatagaacct aatgaaatta cccattgcca
     1861 attgctaaaa tcaaagttag agctaatcat tttatcagtt aagtaagcaa aagggatata
     1921 atcaaaagca tctaaaactt tttcaccgta tcttgggata aatgtaataa aaggtttaat
     1981 aaacggtaca atgagtacta aaaatacacc taatgaaaat atcattgctg ttttttgtga
     2041 tgcaagtgtg attaagaata aaaacaagcc aaagaatagt aaaaagatta ggaagaacca
     2101 taaattatct acatatttag aaaacgccaa attttttcca ttactagatg cctgcacaac
     2161 aatcaaaatc acataatgaa ttaatgcaaa taatattgaa attaaaataa ttgaaattgt
     2221 tttcgaaata atgtatccaa cgcgtgtttt aactttactc atatatagtt gaattgtacc
     2281 ttgtgaaaaa tcacgcgtaa ttgtcttaac aacaaacatt aatccaatta ttaacaataa
     2341 ccattttgct gcagcaaata cagatccata gacaataata tgtttcgggt tatccatcga
     2401 atttgccata agtatacttt gagttgcaat caaactagat acgactagta tcgctaaata
     2461 tgttaaagga cttttaaata tactataaaa atcatactta actaaattta aaattctcat
     2521 tatttatcac ctctttgatt tatattgaag tacgtatcac gcaatgaact tttacgtgtt
     2581 tcaatatatt tcggataaat atctaattca gataatgctt ttagtataac ttgataattt
     2641 ttttgtgcat tgatcattaa atgtccgtct gctttgtcga cattttgtaa ttggaaatgc
     2701 tctgcaagat atttttcagt tctatcaaag tctttatgat caacagtaac tatcgttgtg
     2761 tcagatgcaa caccttcctc catgtttaca tcttgaacaa aatgtccgtc tcttaaaaat
     2821 acagctctat cacaaattaa ttcaatatct tctaacttat gacttgatat tagaattctc
     2881 atatcaagtt catttactaa agacttaatt gtagtcagca catcaattga gccatctgga
     2941 tccataccat ttgtaggctc atccaagatt aaaaatttag gtttattcat taaagatact
     3001 gcaattgcta acttttgctt catccccatt gaatatttct taactttctt tttaatataa
     3061 ggtctcatac caaatgcatc tataattttg tctgtgtatg ctttatcaaa acccttacct
     3121 aatacttgtg caaataattt caagttatac aatcctgatt tattatcata taatttagga
     3181 tgctcaatta aaaaaccgat attatctgca ttatcaatat ctacttttcc atcaaattta
     3241 ataatattac cattcattac tttcataacg gttgttttac caacaccgtt ttttcctatt
     3301 aatccgacaa ttctactatc gccaaagtca aaatcaatat catttaaaac gacatttgag
     3361 ccgtattttt ttgtaatatg ttctaatttc atcgactgcc tcctttaaat tttctagtat
     3421 ttattttatt gagctgttta aa
//
LOCUS       DAKTIP010000226         3405 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.229, whole genome shotgun sequence.
ACCESSION   DAKTIP010000226 DAKTIP010000000
VERSION     DAKTIP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.229"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            269..916
                     /locus_tag="O0305_002504"
     CDS             269..916
                     /locus_tag="O0305_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCY0275609.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            1147..2046
                     /locus_tag="O0305_002505"
     CDS             1147..2046
                     /locus_tag="O0305_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275610.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLKLESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            2039..3271
                     /locus_tag="O0305_002506"
     CDS             2039..3271
                     /locus_tag="O0305_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275611.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGITAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIYEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            3255..>3405
                     /locus_tag="O0305_002507"
     CDS             3255..>3405
                     /locus_tag="O0305_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275612.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLK"
BASE COUNT         1223 a          449 c          612 g         1121 t
ORIGIN      
        1 attttatttg catagattga gactctttta aacgtgaaca ttgcagtatg acatattttt
       61 agtatagtta acgaagcgtt gactttcaaa tgagtctcca tatagatgtg tacttgtagc
      121 gggttttcat tttactagtg atgaaatata aagttcattt tccaaagtga ttgacgattg
      181 aaatgaagtg gcttgataat gaatcttgta aattataacg gtaacaataa ttaaaaatat
      241 aaatatgatt aacgaaaggg agattacaat ggaacaatta cctcaagtag gttttatgaa
      301 atcattcgtt ttattttgga aaaattatgt aaattttaag gggagatcaa gacgtagtga
      361 atattggtat atggcactat ggcatttgat acttatggtt ccagctgtgt gtgtgctaat
      421 aataaaccta tttctattct ttatttctat agcggcggat aacgaaacat taatagttat
      481 gagtatattg atgattatcc ttacatctgt gtatactttt gtctatagtt tggcaacgtt
      541 tattccaaat ttagcgttgt gcgttcgaag atttcacgat atatcaagaa caatgttatt
      601 tccaatgatt atgacagtat tcagtattat attttatata gtattacaga ttatcgatag
      661 ttattatgac agtgatttta cattaatgcc gatgggtctt aatattttgt tagtacttct
      721 ttattttgtt tatttaggcc taacaattgt tatgatagta ttcctatgct ttgacagtaa
      781 gccggcaaat aaatatggcg aaagcccaaa atacccgtct acaattaaaa atcaaccagt
      841 gacatctgaa acaccggaaa aagcgtgtac atctgaaaca ccgaaaacac cggaagaaac
      901 agataatcaa ttgtaatgat ataaatatct cggcacaatg atgctgggat atttttttac
      961 aaataaggca ataaaggcat ggaaaaaaca tatctagtgc taatgatact taacatgcaa
     1021 aaatggcact atttaaattt ttcaatttca gaaattttaa aaataaattc attttaaaaa
     1081 tattgcattt atagagaatg gaaatgttat attaagataa atacatattt ttttaggagg
     1141 caaagtatgg gacttgtaat tgaacatgtt acgaaacgtt ttggcaagat gacggcagta
     1201 aatgatattt cattaaagtt agaatcaggt aaaatgttag gttttctagg aagaaatggt
     1261 gcaggtaaaa caacgacatt tcgtatgatt ttaggcttaa gtgaaccaac tgaaggacac
     1321 attacgtata acggtaaaaa actagataaa acaatgtaca atcgtattgg ttatttaccg
     1381 gaagaacgcg gtttacatgg gaaattgaca gttgaagaag aattgaaata tttggcaact
     1441 ttaaagggaa tgtcaaaaac agaaatacaa cagcaaatat cgtattggct tgagcgtttt
     1501 gatattactg aaaaccgcaa aaaacgaatt gatagtctat caaaagggaa tcaacaaaaa
     1561 attcagctgt tagcaagtat gttacataaa ccagaattac taatattaga tgagcctttc
     1621 agtggtctag acccagttaa tgttgagtta ttaaaagaag cagttaaaga cttaaatgat
     1681 tggggcagta caatcgttta tagttctcac cgtatggagc atgttgaaga attatgtgat
     1741 gatgtttgta ttttagataa aggtcaactt gttgtttctg gtgatatcaa tcatgtacgt
     1801 gcatctaatg gtaacaagaa agttgtcatt gaatcagaaa caacactacc agatttaaca
     1861 aacattagag gcatcattca tagtgaaaac atgaagcaag gtcttcagtt gacgattgag
     1921 aatgaagacg tagccaaaga tatttatcaa gttgtggcac atcaaggtta tgttaaacga
     1981 ttccaagttg tagaaccatc attacaagat atattcattg agaaggttgg tggcaaggat
     2041 gcataagttt tgggcgacat ttactttgac atatatcaaa aaaattaaag ctaaatcatt
     2101 tgtaattttt atgattatta ttgctgcctt aatgattggc ctatctaaca ttgataagat
     2161 tattaacatg tttgatgatg gtccagataa aattggtgta gcagcaccga atgaacaaat
     2221 ctataaggtt ttcaagcaac aggcgaatac atttcatagt gatgctaagt ttactaaagt
     2281 ttctatagaa gatgctgaaa aagaagtaaa aaaacataaa cttgataaag cttatattat
     2341 caaggtgaat caaaatcgca cgttacaagg gacaatcatt agtgagaagc gagtaagtca
     2401 tgaagattca caaaaagtac aagcactgct tacagctatt caaacaaata tggtagccgg
     2461 ggaattaaat atcaacaaag aagacttaca aaaattacaa gcacaaagta aagttgataa
     2521 taaggtgatt tctaatgatg aagtcgataa agtatcagaa ggacaaaaga ttttcaacta
     2581 tgctttagct tatggcatta tctttctaat gttcttcatt gttttaaatt atgcatctca
     2641 aatagctatg gaaattgcta gtgagaaaac atcgcgtgtc attgaaatga ttattacaag
     2701 tatttcacca atccaacata tttttgctaa aattttaggc gttatagcag ttgccgtgac
     2761 tcagattatt ttaattgtat tgatggcagt tatatgcatt tttgcctttg acttaaaaga
     2821 cttgctacaa ggttttaacg tggaaatgaa tcaattgtct tggcaaatta ttattgtcgg
     2881 aattataagt agtattgttg gaattacggc gtatgtatta cttgcagcca tcctcggatc
     2941 attaacatct agaatagaag atttaaatca atctttgatg ccactgacat tacttggtat
     3001 gattgcattt tatatcgcga tattcacgat aaataatcct gatggacaac ttgctttaat
     3061 cacaagttat attccgtttt tagcgccatt tcaattagtt gtacgcgctc aaacttcagg
     3121 gcttcaaata tatgaggtca tcttaagttc tttaatctca attgtaatgg tagctgtctt
     3181 gatatggatt gcaattaaaa catataaaga ttcagtactt acatttgaac gtggcttatt
     3241 taattcaatg aaacgtgtat ttaaaaaata aaattgatga gagtgacaca catttatcat
     3301 ataaaatgat gatgaaagcg tgttactctt atttttttat atttagctat agaataatgt
     3361 ctaaaaaagt gtacaatatg aatgtgaaaa cgaacttttt aaaat
//
LOCUS       DAKTIP010000227         3405 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.230, whole genome shotgun sequence.
ACCESSION   DAKTIP010000227 DAKTIP010000000
VERSION     DAKTIP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.230"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..670
                     /locus_tag="O0305_002508"
     CDS             <1..670
                     /locus_tag="O0305_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCY0275613.1"
                     /translation="WVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKG
                     GYVLVEWHGITIETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLK
                     MFKLPVHQLSMIMSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLV
                     PLFISAFQRAEELAVAMEVRGYDANVKRTSYRQLKWQLRDTISLTMIIPIAIILFVLK
                     YSGV"
     gene            675..1478
                     /gene="truA"
                     /locus_tag="O0305_002509"
     CDS             675..1478
                     /gene="truA"
                     /locus_tag="O0305_002509"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCY0275614.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            1718..2155
                     /gene="rplM"
                     /locus_tag="O0305_002510"
     CDS             1718..2155
                     /gene="rplM"
                     /locus_tag="O0305_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCY0275615.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            2169..2567
                     /gene="rpsI"
                     /locus_tag="O0305_002511"
     CDS             2169..2567
                     /gene="rpsI"
                     /locus_tag="O0305_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCY0275616.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            3112..>3405
                     /locus_tag="O0305_002512"
     CDS             3112..>3405
                     /locus_tag="O0305_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0275617.1"
                     /translation="MRGDQRTQGEKSRKEGGKIFGSGSRAPIAISILVKDGSYNHDIY
                     YNDIGEYLTREQKLDTLMKHQSIVNLKSLNVLPDKNNDWINQRDINYENYLPMY"
BASE COUNT         1248 a          516 c          621 g         1020 t
ORIGIN      
        1 ttgggtgttt gcacttatct tattcattat gagattagca aaaattcaat tatggttcct
       61 aatcaaaggt ttaacaccaa tatttttctt tttaatcttc acattaatga tgcatatatt
      121 tttaactaaa ggtggatatg tattagttga atggcatggc atcacgattg aaactaatgg
      181 tattttagaa gggctatata tatcactgcg tttaattggg attgtaatga ttgcaacaat
      241 tatgacacta tctacaagtc caattgattt aacagatgcg tttgaaagat tacttgcacc
      301 actaaagatg tttaaattac cagttcatca attaagtatg ataatgtcca ttgcgttacg
      361 attcatcccg acgttaatgg atgagttaga taaaatcatt ttggcgcaaa agtcgcgtgg
      421 ttctgaaata agttcaggga acattgcaac acgtattaaa tcatttattc cattactagt
      481 gccactattc atctcagctt tccaacgcgc cgaagaatta gcggtcgcaa tggaagttag
      541 gggttatgat gccaatgtta agagaacgag ctacagacag cttaaatggc aattgagaga
      601 tacgatatct ttaaccatga ttatcccaat tgcaattatt ttattcgtat taaaatattc
      661 aggagtgtaa attcatgcgt atattagtag aaattgcgta tcaaggaaat aattttctag
      721 gttttcaaat tcaacaaaat ggtcgaacgg tacagcaaca atttgaaaag ctattacaac
      781 gcatgcataa aagacatgtg agaatccatc cttcaagtag gactgataga ggtgtacatg
      841 cgatacagca atactttcat ttcgacacag agctgaatat acctatgtca caatggcaat
      901 atgcaatgaa ccgaacgtta cctgatgata tttatgtcaa caatgtagtt acagtcgatg
      961 acgattttca ttgtcgttat gattgtgtag gtaaacgtta tcgctacaaa gtatatcaag
     1021 cacaacaccg cgatccattt caaagtggtt tgaaaacatt tattcctgaa acgttggatt
     1081 taggtaaaat gaacagagcg gcgcaacaat tcataggtac acatgatttt acgggatttt
     1141 gttcgcaaaa aactgaagta gaaagcaaag ttagaacatt ataccaaagt gaaatcgtaa
     1201 agacagatga tggctttgat tatattgtca ctggatctgg atttttatat aacatggtgc
     1261 gtgtcttagt tgcattttta atagaagttg gaaaagggcg acatgaagta tctgatgtac
     1321 caaaacttct tgaatcaaag aatcgtaaga atgtaccttt cactgcaccg gctgaaggat
     1381 tatatttaga gaaaatttat ttagatgaaa atgagttatt aaaagacttt ggtaacgata
     1441 tcaaaataca tcgtaaaaaa tcattacaaa atgactgatt gccattgaca aaaagcttgt
     1501 gaaattataa gattattaac ggtattgttt tatatccacc ccacgataag ccccggaaac
     1561 ttattgtgtt acaagatata taagcagaaa cgaacaacag ttaacaaaat aaatgaaatt
     1621 aaacgtttta aaaatgaaac aaatgaaatc atctattagg ttatgaaact gtttatagct
     1681 tgaatagaag catttatttt ttaggaggac aattattatg cgtcaaacat ttatggcaaa
     1741 tgaatcaaac attgagcgca aatggtatgt tatcgatgct gaaggccaaa cattaggtcg
     1801 tttatcatca gaagtagcat ctatcttacg cggtaaaaat aaagtaactt acacaccaca
     1861 cgttgatact ggtgattatg taatcgttat taatgcatca aaaatcgaat ttactggtaa
     1921 caaagaaact gacaaagttt actaccgtca ctcaaatcac ccaggtggta tcaaatcaat
     1981 cactgctggt gaattaagaa gaactaaccc agaacgttta attgaaaact caattaaagg
     2041 tatgttacca agcactcgtt taggcgaaaa acaaggtaaa aaattatttg tatatggtgg
     2101 cgctgaacat ccacacgctg cacaacaacc agaaaactac gaattacgtg gttaattaga
     2161 aggaggaaat gactttggca caagttgaat atagaggcac aggccgtcgt aaaaactcag
     2221 tagcacgtgt acgtttagta ccaggtgaag gtaacatcac agttaataac cgtgacgtac
     2281 gcgaatactt accattcgaa tcattaattt tagacttaaa ccaaccattt gatgtaactg
     2341 aaactaaagg taactatgat gttttagtta acgttcatgg tggtggtttc actggacaag
     2401 ctcaagctat ccgtcacgga atcgctcgtg cattattaga agcagatcct gaatacagag
     2461 gttctttaaa acgcgctgga ttacttactc gtgacccacg tatgaaagaa cgtaaaaaac
     2521 caggtcttaa agcagctcgt cgttcacctc aattctcaaa acgttaattg tcggacgata
     2581 tatacaaaac acctcgatat tatgtcgagg tgtttttttg cgtttttgcg gcgaatatgg
     2641 aatgtgtaga atataaatga atttttacct tcccaccata aaagatgaag aaccatgaaa
     2701 tgtggagaac aataaatagt tgatattctg ttattttttt ggaagtgaag tgatttgaat
     2761 actttactct aaacgataaa aggttaaaaa aacaacaaaa agaaaatatt actgtcataa
     2821 ttggaaatac accgtattct gctggtcaat ctagtgtaaa taatgataat aaaaaaatga
     2881 atatgaaaaa ttaaatgcaa aaatagcaaa tacctatgca aatttttcaa cagcaaattt
     2941 aaaaaaatca ttatatgatt cgtatataca ggcttttcga tgggcaactg atagagttgg
     3001 aaaaaaggta ttataggatt tgtaacaaat ggatcattta ttgatagtca aagcactgat
     3061 ggatttagaa aagttttata cgatgaattt aactatctgt acataattaa tttgagaggc
     3121 gaccaacgaa cacagggtga aaaatcaaga aaagaaggtg gaaaaatatt tggatcagga
     3181 agtagagcac cgatagctat atctatttta gttaaagatg gaagttacaa tcacgatatc
     3241 tattataatg acattggtga atatttaact agagagcaaa aattagatac tttaatgaaa
     3301 catcaatcaa ttgtgaattt gaaaagtttg aatgttttac ccgataaaaa caatgattgg
     3361 attaatcaac gagacataaa ctatgagaat tatttaccaa tgtat
//
LOCUS       DAKTIP010000228         3395 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.231, whole genome shotgun sequence.
ACCESSION   DAKTIP010000228 DAKTIP010000000
VERSION     DAKTIP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.231"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            140..775
                     /locus_tag="O0305_002513"
                     /pseudo
     CDS             140..775
                     /locus_tag="O0305_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            772..1302
                     /gene="sat4"
                     /locus_tag="O0305_002514"
     CDS             772..1302
                     /gene="sat4"
                     /locus_tag="O0305_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0275618.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1395..2189
                     /locus_tag="O0305_002515"
                     /note="aph(3')-IIIa"
     CDS             1395..2189
                     /locus_tag="O0305_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0275619.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2465..3036
                     /locus_tag="O0305_002516"
                     /pseudo
     CDS             2465..3036
                     /locus_tag="O0305_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(3205..>3395)
                     /locus_tag="O0305_002517"
     CDS             complement(3205..>3395)
                     /locus_tag="O0305_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCY0275620.1"
                     /translation="NNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRS
                     LQIYGFSPCHEISIMLAS"
BASE COUNT         1125 a          538 c          764 g          968 t
ORIGIN      
        1 cgaacactga catgatatat tagtgattag ctatattttt tactttgcaa cagaaccatc
       61 atttttatta aaatactctt ttgtaaaatc tttctgctct tcaatgtatt taatttcttt
      121 acctctaaat ctattatagc tatttgatga ttacaataaa attgatctta ccttattgcc
      181 cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc taattgataa
      241 agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg taagaaagcc
      301 aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac cttatgttat
      361 taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc agattgttcg
      421 ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct ttaaatgaag
      481 tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt gggagaaact
      541 tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat ttctatgcca
      601 tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg cctatccgga
      661 gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca ctggtaaaac
      721 cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac agtgattaca
      781 gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt tgaggtgata
      841 ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa
      901 gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc
      961 cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga cgataaatgc
     1021 gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga agatatcgcc
     1081 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1141 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1201 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1261 gccaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     1321 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     1381 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     1441 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     1501 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     1561 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     1621 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     1681 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     1741 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     1801 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     1861 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     1921 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     1981 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     2041 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     2101 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2161 attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa aaagctttaa
     2221 ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt ccatactctg
     2281 atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag atgtaaagag
     2341 ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga gaagcactaa
     2401 cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag aggaggaata
     2461 aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat caaaaaaata
     2521 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     2581 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     2641 cttgtagagg gcttcctact tctgccggta atgtttacag cggcggaagt gggtgcattg
     2701 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     2761 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     2821 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     2881 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     2941 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     3001 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     3061 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     3121 tggaacaaat attaaaacaa atgctggttc tgttgcaaag taaaaaaata tagctaacca
     3181 ctaatttatc atgtcagtgt tcgcttaact tgctagcatg atgctaattt cgtggcatgg
     3241 cgaaaatccg tagatctgaa gagacctgcg gttcttttta tatagagcgt aaatacactc
     3301 aatacctttt aaagtattct ttgctgtatt gatactttga taccttgtct ttcttacttt
     3361 aatatgacgg tgatcttgct caatgaggtt attca
//
LOCUS       DAKTIP010000229         3312 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.232, whole genome shotgun sequence.
ACCESSION   DAKTIP010000229 DAKTIP010000000
VERSION     DAKTIP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.232"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..242
                     /locus_tag="O0305_002518"
     CDS             <1..242
                     /locus_tag="O0305_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HCY0275621.1"
                     /translation="SSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYSPEAFAG
                     FAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            347..463
                     /locus_tag="O0305_002519"
     CDS             347..463
                     /locus_tag="O0305_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275622.1"
                     /translation="MANYAIYFNNININSIMALNDNNYQFEHVNERNEYRES"
     gene            474..1508
                     /locus_tag="O0305_002520"
     CDS             474..1508
                     /locus_tag="O0305_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0275623.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDRHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            1544..1945
                     /locus_tag="O0305_002521"
     CDS             1544..1945
                     /locus_tag="O0305_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275624.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDKDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIKY"
     gene            complement(1991..2995)
                     /locus_tag="O0305_002522"
     CDS             complement(1991..2995)
                     /locus_tag="O0305_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0275625.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            3186..>3312
                     /locus_tag="O0305_002523"
     CDS             3186..>3312
                     /locus_tag="O0305_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275626.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFK"
BASE COUNT         1092 a          503 c          635 g         1082 t
ORIGIN      
        1 tatcaagtta ctacttattt aactggttct tctatgtata taacataggt gttatcgtta
       61 ctataggtat gatggtgaca aaaggattct tccaagtcac tggtaaatca tactctccag
      121 aagcatttgc aggctttgca ggtattgggc acacaggtat gcttgcaggt ttattgttgc
      181 tatttttcct tttaagacaa gcaattctta aagaaccacg tgatgagtct tttaaaaaat
      241 aatataacta gaactaaaaa atatacacat ctttcaaagg aacattgttg tttcttactt
      301 tgaaaggtgt gtttttatat tgtgatggga tagttattga taattcatgg ctaactatgc
      361 gatttatttt aacaatataa atattaatag tataatggct ttaaatgata ataattatca
      421 atttgagcac gttaatgaaa gaaatgaata tagagagtca taggaggcac ataatgaaag
      481 atgttacaat cattggtggc ggtccgtctg gtttgtatgc aagcttttat gctggattgc
      541 gagatatgtc tgtaagattg atagatgttc aatctgaatt agggggtaag atgagaattt
      601 accctgaaaa aattatttgg gatattggcg gcattgcgcc aaaaccttgt catgaaattt
      661 taaaagatac aattaagcaa ggattatatt ttaaaccgga agttcatttg aatgaacgtg
      721 tggtagatat taggaaaaag gcagaacgtc atttcgaagt tgaaacagaa gcgggagaaa
      781 tatatacatc gaaagcagtt atcattgcaa ttggtgcggg tattattaat ccaaaacaat
      841 tagatgttaa aggtgtagag agatatcaat taactaattt acattatgtt gtacaaagtt
      901 acagacgttt caaagataaa gatgtattaa tatcaggtgg aggaaataca gcattagatt
      961 gggcgcatga tattgccaag attgctaaaa gcgtgacagt tgtttatcga aaagaagatg
     1021 taagtggtca tgaagcgatg aaaacgttgg tgacagattt aaatgtgaaa ctatgcccaa
     1081 aaacacgtat taaatacttg gtcggcaatg atgatgaaac gcatataagt gaagttgttt
     1141 tggaacatgt tgaaagtggc gataggcata cagttaaatt tgacgatgtc attattagtc
     1201 atgggtttga tcgttgtaat acattattga gtgaaacatc ttcaaagcta gatatgcatg
     1261 atgattgtcg tgttaaagga tttggtaata cgacaactag tatacctggt atctatgcgt
     1321 gtggagatat tgtttatcat gatgcaaaat cacatctaat tgcaagtgca tttagtgatg
     1381 gtgcaaatgc agcgaacctt gctaaaacat atatccaacc agatgcgaat gcagagggtt
     1441 atgtttcaag tcatcatgaa gtgtttaaag aagcgaataa gactattgta aataaacatt
     1501 tatactaggt tgatatattg tgtgatgaaa agtggggtaa ataatggtta aagtgacata
     1561 tgatattccg acttgcgagg attattgcgc attaaggatt aacgcaggta tgagtccaaa
     1621 gacgcgcgaa gcagctgaaa aaggattacc taatgcctta tttacagtaa ccttgtatga
     1681 taaagatcgg ttaattggta tgggtagagt gattggcgat ggcggaactg tttttcaaat
     1741 tgttgatatt gcagttttga aaagttacca aggtcaaggt tacggcagtc taattatgga
     1801 gcatattatg caatatatta aaggtgtggc tgttgagagt acatacgtta gtctgattgc
     1861 agactaccca gcggataaat tatatacaaa atttggattt atacctaccg aaccagattc
     1921 aggcggtatg tatatcaaat actaaattaa aaaagacatc tcgcataacg ggatgtcttt
     1981 ttcatgaata ctatttatct acaccgaaat cgataacaat gcgcccttta ttttcatgtt
     2041 taattacttt gttaagttgt tctggaagtt catcaaatgt aataacttgc ttgatctcat
     2101 gtaaattttc aggcattaaa tcttttgcga gacgacgcca aacgcgctgt cttaatttca
     2161 tagcagtaaa taccgagtca attcctaaaa tgtttacacc tcttaaaatg tgagggaata
     2221 cagaattagt ataagtatta ccggcagtca taccaataac tgcaattgac ccactatgat
     2281 ttaaacgctt tgtaacataa ttaatacctt cgccaccaac agggtctaca caagcttgcc
     2341 aagttgatga tgcgagtggc tttttatgat catcttcaac aggaagtcga tcgataactt
     2401 ctttggcacc aagttcaagt aattgatcgc taacatcttg tttacctgta cttgcgataa
     2461 ctttataacc aagttcatta agcatgagta ctgctaaagt accgacacca cctgaagcac
     2521 cgcgaacgag tacaggacca tcttcaatat tcattccaac tttttcaagt ctttcaattg
     2581 ctaaaccggc agtataacca gctgtgccat atatcattga ttcttctaat gttaaagtat
     2641 caggaagctt gataatccat tctgatttta cacgcgcata ttcactaaaa ccgccataat
     2701 ggctgacacc taggtcataa ctcgttacaa ttacttgttc tcctttttca aagcctggtg
     2761 cttcggattc aacaattgtt ccagctaaat ctattcctgg aatcatagga tacgatttta
     2821 cgactgcatt atgatcttga gtcgctaaag catctttata atttatacca gagtaatgta
     2881 ctttaatcag cacatctcct ttaggtaaat cagtaggcga taattgtttg aaagttggtg
     2941 tcactttacc actctcatct ttatcaatta caaacgcttt aaatgtttct atcacaacga
     3001 tacatcctct ctttttctct tttgaaaata cggccatttt actggaataa atgtatcatt
     3061 tattgatgtt acacacaatc aatacaattt gtaattgatt tgtgttaatg tatttttaaa
     3121 tagttttggc tgaaaaataa cataagtaat attttgattc gatcactgtt tattggagga
     3181 tttatatggc tgggataatc aaagaaattt cagaacaaaa tgctagtgaa ttagttgaat
     3241 tagcaactag aacattttat gacacgtttg gttcttacta tgatgacaaa gattttgatc
     3301 aattttttaa ag
//
LOCUS       DAKTIP010000230         3295 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.234, whole genome shotgun sequence.
ACCESSION   DAKTIP010000230 DAKTIP010000000
VERSION     DAKTIP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.234"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            234..1442
                     /locus_tag="O0305_002524"
     CDS             234..1442
                     /locus_tag="O0305_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCY0275627.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            1573..3048
                     /locus_tag="O0305_002525"
     CDS             1573..3048
                     /locus_tag="O0305_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCY0275628.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKTRSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVFYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSEVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
BASE COUNT         1187 a          465 c          637 g         1006 t
ORIGIN      
        1 ttttcacaat ctgactgaat cttgcttgaa aatataacat aaaagctata gaattagtat
       61 tggattaaaa atgtcttttt tgtgaacatc acaggttttt acataattta aaagacttaa
      121 ataacaaaat gatgataaac tagtatacat tgtggtaaaa gtagtaatga ccagttgtta
      181 ctagtaatta tcagcatttg gttaaaaaaa tatgaaagct taggtgaaat taaatggctc
      241 aagatcgtaa aaaagtactt gtacttggtg ctggttatgc aggtttacaa actgtaacta
      301 aattgcaaaa agcgatatca acagaagaag cagaaattac gcttattaat aaaaatgaat
      361 atcactatga agcaacatgg ttacatgaag catcagcagg tacactaaac tatgaagatg
      421 tattatatcc tgtggaaagt gtcttgaaga aagacaaagt gaactttgtt caagcagaag
      481 taacaaaaat tgaccgtgat gctaaaaagg tagaaacaaa tcaaggtatt tatgactttg
      541 atattttagt agtagcatta ggtttcgtta gtgaaacatt cggcatcgaa ggtatgaaag
      601 atcatgcttt ccaaattgaa aatgttatca cagcacgtga attatcacgt catatcgaag
      661 acaaatttgc taactatgca gcatcaaaag aaaaagatga taacgattta tctatcttag
      721 ttggtggtgc tggattcact ggtgttgaat tcttaggtga attaacagac agaattcctg
      781 aattatgtag caaatatggt gtggatcaaa ataaagttaa aatcacttgt gttgaagcag
      841 cacctaaaat gttaccaatg ttctcagaag aattagttaa ccacgcagtt agctacttag
      901 aagaccgcgg tgttgaattt aaaattgcta caccaatcgt tgcttgtaac gaaaaaggtt
      961 ttgtagttga agtagatggt gaaaaacaac aattaaatgc aggtacttca gtatgggcag
     1021 ctggtgtacg tggtagtaaa ttaatggaag aatcatttga aggcgttaaa cgtggacgta
     1081 tcgttacaaa gcaagattta acaatcaatg gttacgacaa catttttgtt attggtgact
     1141 gttcagcgtt tatcccagct ggagaagaac gtccattacc aactacagca caaattgcaa
     1201 tgcaacaagg tgaaagtgtt gctaaaaaca ttaaacgcat cttaaacggt gaatcaactg
     1261 aagaattcga atacgttgat cgtggaactg tttgttcttt aggttcacat gacggtgtag
     1321 gtatggtatt tggtaaacct atcgctggta aaaaagcagc attcatgaaa aaagtgattg
     1381 atacacgtgc ggtattcaaa atcggtggta tcggtttagc attcaaaaaa ggtaaattct
     1441 aggctttaaa taaagtttca gctaaacaca taaaattaaa ttattcattt ataaagtcct
     1501 tcagtgtcac tgaagggctt tattgttgta caatttgaag tgagtaacat atcaaatcta
     1561 aggaggtaca ttatgaattt taaattaaat aacacactaa gcaacgaaat aaatacattg
     1621 attattggta taccagaaca tttaaatcag ttagagcgca ttagttttaa tcatatcgat
     1681 attacagaat cacttgaaag actaaaacat caacatatta ttggtagtaa agttgggaag
     1741 atttatacaa ctgcatttga tgtacaagat caaacatatc gtttaattac agttggttta
     1801 ggaaacttaa agacacgtag ttatcaagat atgttgaaaa tatggggaca tcttttccaa
     1861 tacataaagt cagaacacat tgaagatacg tatttactta tggattcatt tatttcaaaa
     1921 tatgatcagt tatcagatgt attaatggca tgcggtattc aaagtgagcg tgcaacatat
     1981 gaattcgatc attataaatc aagtaagaag gcaccgttta agacgaattt aaaccttatt
     2041 agtgaatcat taattgaatt agattttatt catgagggta tcagtattgg ccaatccatt
     2101 aatttggcaa gagactttag taatatgcca ccgaatgtat taacaccaca aacatttgca
     2161 gaagatattg ttaatcattt taaaaataca aaggtcaaag tagatgttaa agattatgac
     2221 actttagttt ctgaaggatt cggactttta caagcagtag gtaaaggtag taagcataaa
     2281 ccgagattag taaccatcac atataatggc aaagacaaag atgaagcacc aattgcctta
     2341 gttggtaaag gtataacgta tgattctggt ggttatagta ttaaaacgaa gaatggcatg
     2401 gctacaatga agtttgacat gtgtggcgct gcgaatgtcg ttggtatcat tgaagcggct
     2461 agtcgtttac aactgcctgt aaatattgtc ggagtgcttg cgtgtgctga aaatatgata
     2521 aatgaagcat caatgaagcc agatgatgta tttacagcat taagtggtga aactgtagaa
     2581 gtaatgaata cagacgctga aggtagatta gtccttgcag atgctgtgtt ttatgcaaat
     2641 caatatcagc ctagtgtgat tatggacttt gctacattaa cgggtgcagc aattgttgca
     2701 ctaggcgatg ataaagctgc tgcatttgaa tcgaatagta aagtgatatt aaacgatata
     2761 ttacaaataa gttctgaagt cgatgaaatg gtatttgaat taccgattac tgcaaccgaa
     2821 cgtgcaagta ttaaacacag tgatatcgct gatttagtta accatacgaa tggacaaggt
     2881 aaagcgctat ttgcggcaag ttttgtaaca cattttagtg gtcaaacacc tcacattcat
     2941 ttcgatattg caggtccagc aacgactaat aaagcttcat ataatggtcc aaaagggcca
     3001 acaggattta tgattccgac gatagtacaa tggttaaaac aacaataatt taattatttg
     3061 cctgatacaa cttgtgtctt gatatacatt tctaatttaa ctcttgaaag tataaaggat
     3121 cgcattgtat caggcatttt cattgttgta agaaaatata aatcgcatat ccttgtgagc
     3181 ataaatcaac ttgactagca ttgcaaaaaa atattataag catatgttca ttcagtcaac
     3241 gactactaat attgcatttt agaacttagg attttgattg gtgttccaag cttgt
//
LOCUS       DAKTIP010000231         3293 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.235, whole genome shotgun sequence.
ACCESSION   DAKTIP010000231 DAKTIP010000000
VERSION     DAKTIP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.235"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            239..811
                     /locus_tag="O0305_002526"
     CDS             239..811
                     /locus_tag="O0305_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCY0275629.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDLYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
     gene            814..1539
                     /locus_tag="O0305_002527"
     CDS             814..1539
                     /locus_tag="O0305_002527"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCY0275630.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            1541..2500
                     /locus_tag="O0305_002528"
     CDS             1541..2500
                     /locus_tag="O0305_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0275631.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            2592..3041
                     /gene="ndk"
                     /locus_tag="O0305_002529"
     CDS             2592..3041
                     /gene="ndk"
                     /locus_tag="O0305_002529"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCY0275632.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
BASE COUNT         1223 a          423 c          616 g         1031 t
ORIGIN      
        1 attgttagcg ttgaatttac ttaaaaggta accatgtcta ctatagtatt ttacgttatt
       61 taaaaagatg aataatgtaa atgaagtaaa ggttattatg agaattacaa aagctacata
      121 aattactgtt agtttaaatt gaaatttaaa aatgataagt aatcttaatg cttcaaaaga
      181 ttttaatttc ttacatataa tgttaatatg aatagtgata atatcgtgag gtgtagacat
      241 ggaaacaact gttagcaaat tggaaagaca aatagaagaa agattaaagg gcgtatcaga
      301 atatgaatcc attaatatta atcatcggtt aggtaagtta cttgatttat atgatattcc
      361 agatgttgcc aaagtggcat gcctcacaat tgacacatca atgagacacc ttgatgacat
      421 tacatataat catttgtcta aacattccat tttgattgga gatttaatta gtgcacattt
      481 ttatacgcta ctagcagaaa tcaatgattt atcatttcaa aatgaaatta gtaaagcaat
      541 tgttgaaatt aatgaattga aatcttcatt acatcatcaa gctttaaatg attatgaaat
      601 ttctcaagca atagtaaaaa ttgaaacact tttcccatat attacgttat ctcatttcgg
      661 tatcaatatt gatgagtcgg aaatatataa ttatttattt gaagatatgt ctgattacta
      721 tccatcatac ttcaagaaat ataatcaaag tgaagtaaag cattacttac acgatatcca
      781 aaaaagttat ttaaaaagta gaggtaatta agcatggctg acaataaagc aaataaagag
      841 caagtacatc gcgtttttca aaatatttca aaaaaatatg atagattaaa taatattatt
      901 agttttgagc agcataaagt atggagaaaa cgcgtcatga aagacatggg tgttagaaaa
      961 gggacgaaag cattagatgt ttgttgtggt actggtgatt ggacaatcgc attaagcaaa
     1021 gctgtaggac caacaggtga agttactggt attgacttta gtgagaatat gttagaagta
     1081 ggaaaagaaa aaactgcttc aatggaaaat gttaaacttg ttcatggtga tgcaatggaa
     1141 ttgccgtttg aagacaattc ttttgattat gtaacaattg ggtttggatt aagaaatgtg
     1201 ccagactatt tagtcgcgtt aaaagaaatg aatagagtac ttaaaccagg tggtatggtg
     1261 gtatgtcttg aaacgagcca accaactttg ccagtcttta aacaaatgta tgcactatac
     1321 tttaaatttg ttatgcctat ttttggaaaa ttatttgcaa aatcaaaaga agaatatgaa
     1381 tggttacagc aatctacgtt taattttcct ggaaaagaag agttgaagcg catgtttgaa
     1441 gaagcaggtt tcataaatgt aagagtacgt agttttacag ggggcgttgc tgcaatgcac
     1501 cttggctata aagaaaaaga taataccaaa ggtgattaac gtggcaaagt taaacatgaa
     1561 caatgaaatt aagaaagtgg aacaacgact tgaaaaggca ataaaaagta aagattctgt
     1621 attagaacag gcatcattac acttattgtc ttctggtggt aaaagagtac gcccagcatt
     1681 tgttattctg agtagccaat ttggcaaaga tgaacaaacg tctgaacaaa cgtatcaagt
     1741 tgcagtcgca ttagagttaa ttcatatggc aacacttgtt catgatgacg ttattgataa
     1801 aagcgacaag cgtcgaggca agttaaccat atcaaagaaa tgggatcaga caactgctat
     1861 tttaactggg aattttttat tggcattagg acttgaacac ttaatggccg ttaaagataa
     1921 tcgtgtacat caattgatat ctgaatctat cgttgatgtt tgtagagggg aacttttcca
     1981 atttcaagac caatttaaca gtcaacagac aattattaat tatttacgac gtatcaatcg
     2041 caaaacagca ctgttaattc aaatatcaac tgaagttggt gcaattactt ctcaatctga
     2101 taaagagact gtacgaaaat tgaaaatgat tggtcattat ataggtatga gcttccaaat
     2161 cattgatgat gtattagact tcacaagtac cgaaaagaaa ttaggtaagc cggtcggaag
     2221 tgatttgctt aatggtcata ttacgttacc gattttatta gaaatgcgta aaaatccaga
     2281 cttcaaattg aaaatcgaac agttacgtcg tgatagtgaa cgcaaagaat ttgaagaatg
     2341 tatccaaatc attagaaaat ctgacagcat cgatgaggct aaggcagtaa gttcgaagta
     2401 tttaagtaaa gctttgaatt tgatttctga gttaccagat ggacatccga aatcactact
     2461 tttaagtttg acgaaaaaaa tgggttcaag aaacacgtag tatttatgaa aagtattgaa
     2521 agcgctttac caacctgtta atatataata gtaatataca taaacctatt aaacacaggg
     2581 ggatacataa agtggaacgt acatttttaa tgattaaacc agatgcagta caaagaaatc
     2641 taattggtga agtaatttca agaattgaaa gaaaaggact aaaacttgtc ggtggtaaat
     2701 taatgcaagt accaatggaa cttgctgaaa cacattatgg tgaacaccaa ggtaaaccat
     2761 tttataatga tttaatttca tttattacat cagcaccagt gttcgcaatg gtagttgaag
     2821 gtgaagatgc agttaatgta tctagacata ttattggcag caccaatcct tcagaagctt
     2881 caccaggatc aattagaggt gatttaggtt taactgttgg tagaaatatc attcacggtt
     2941 cagattcatt agagtctgct gaacgtgaaa ttaatctatg gtttaatgaa aatgaaatta
     3001 ctagctatgc ttcaccacgt gatgcatggt tatatgaata aaatataaac tgtaaacctt
     3061 tacgatttat ttataaaggt agaaagggtt ttgttatgtg gttagtcatt atgattatac
     3121 ataacaaggc ccgtttttta tgttgtagta aattacttga aaaattttat agttttttgg
     3181 taacacgtat taaaaagaga ggaatattct ttatcaaatg aaactaaaca gagagaaggg
     3241 gttgttaaaa tgaagaatat tatttcgatt attttgggga ttttaatgtt ctt
//
LOCUS       DAKTIP010000232         3216 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.237, whole genome shotgun sequence.
ACCESSION   DAKTIP010000232 DAKTIP010000000
VERSION     DAKTIP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.237"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(278..2887)
                     /gene="adhE"
                     /locus_tag="O0305_002530"
                     /gene_synonym="adhC"
     CDS             complement(278..2887)
                     /gene="adhE"
                     /locus_tag="O0305_002530"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCY0275633.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQKTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          937 a          595 c          490 g         1194 t
ORIGIN      
        1 cgtttgaaca attaattact tttaaaccgc attattttcg cctttttatt tatatgaaat
       61 taaaaataat tatactctaa agtagtaata gtttgtgaaa tgagtatcaa tcttaatatt
      121 ttctttattt aaaaaaatga ttgtctagtt tgtatctctc tgaagatttg gcaataaata
      181 aaagccgata accgtataat gattatcgac ttaaagttta tgtggcattt tttacttttg
      241 taatttcagg tgagttagat gattattatc agatagatta ttgcttataa tcatatgatg
      301 tttgaatgat atctttgatt tcactgatta gtgcttcttt aggattagca gttgtacatt
      361 gatcttcaaa tgcgagctct gccattctat caattgactc atttaattct tcttcagaca
      421 caccttgtga tttcaaattc atttcaattc cgactgattg acctaattcg tagacagctt
      481 tagctaatga ttctacgagt gcttctgtcg tattaccttt taatcctaag aatttggcaa
      541 tatctgcata atctgtatct gctctgaaga actcatattt agggaataat gcatgttttt
      601 gcgggtcttt ggcattataa cggataatat gcggtagtaa tatcgcattc gctctaccat
      661 gcggaatacc atattcgcca ccaattttat gcgcaattga gtgtgcaatg cctaagaatg
      721 catttgcaaa tgccatacca gccaaagttg atgcgttatg cattttctct cttgaaactt
      781 tatcaccctt ttcaacagat gattttaaat attcgaacgt caatttaatc gcttgtagac
      841 tcaaacctct tgtgtagtct gaagccatta cagatacata tgattccatt gcatgcgtta
      901 gtacatccat tcctgtatct gctgtaacgc tttttggcac actcatcaca aattgagggt
      961 caataattgc aacgtcaggt gttaaagcaa aatcagccaa cggatatttt acatttgttt
     1021 cactatctgt gataactgca aatggtgtta cttctgaacc tgtacctgat gtcgtaggga
     1081 tacaaatgaa cgtcgcattt tcaggcatgc ctattttata agtacgttta ccgatgtcta
     1141 ggaacttttg tttagcaccg aagaatgatg tctcagggtg ttcaaagaac atccacattg
     1201 cttttgcagc atccatcgct gaaccaccac caagtgcaat gattgtatcc ggttggaaat
     1261 caaccatcat ttccagacct ttatatactg tattagttga tgggttcggt tcgacttcgc
     1321 taaatatttt aatttgaggc tgttccgttt tttgtcttaa tacattctca actgtttttg
     1381 tataaccgaa ttctaccata ccagggtcac agacaatcat cactttttca atcttgtcca
     1441 ttgttgttag actcatgatt gcattttctt caaaataaat ttgagcaggc accttgaaaa
     1501 tttgagtatt attacgtcgt ttagcaatcg ttttaatgtt taataaatct gtcgcactaa
     1561 cattatgtga aattgagttt ctaccgtagg aaccacaacc taatgttaaa gacggaatca
     1621 attcgttata catatcacca atacctccaa ccgctgatgg tgtatttaca agtacacgac
     1681 aagctttcat tcttagtcca aaatcttttt gtaatgtttc atcttctgta tggataacgg
     1741 ctgtgtgtcc taatccacca aaatgtagtg tgtcttcaca aatttgaaat gcttgttttg
     1801 tagattgggc ttttactaag gctaatactg gagataattt ttcacgagat aacggatagt
     1861 ctgaacctac accgctaatt tcggctatga taagttttgt attttcgggg acaggtatac
     1921 ctgctaattc agctatttca actgcagatt taccgacaat atcaggctta atacctgttt
     1981 tttgttcatt cataattgca ttttctaagc gttgtaattc atctttttta acaaagtatg
     2041 cttgatgtgc tttaaattca ttagtaacat ctttataaat ttctttatca atgactacaa
     2101 cttgttcaga agcacaaatc ataccattat caaatgtttt tgaaccaatg atatcattta
     2161 ctgcacgttt aatgtgtgct gttttttcaa tgtaagacgg cacgttacct ggtcccacac
     2221 ctaatgccgg tttgccagtt gaatatgcag acttaaccat gcccgaacca cctgttgcta
     2281 gaactaatgc aatacctttg tgattcatta attgttttgt tgcttcgata gaaggcactt
     2341 caatccactg aataatatct ttaggtgcac ctgccttcat tgccgcttct aatacaactt
     2401 ctgctgcacg cttcgacgat tcttgtgcac ttggatggaa tgcaaaaatg attggatttc
     2461 ctgtcttaat tgcaatcatc gctttaaaaa tagttgtcga cgtaggattt gttgttggcg
     2521 taacaccaca aataacacca attggttccg ctacatacgt taatcctttt tctttatctt
     2581 caccaataat ccctactgtc ttattgtctt ttattgaatt ccatatatat tcagaagcgt
     2641 ataaattttt aatcgcttta tcttcgtata tacctcttcc agtttcttca tgtgctaatt
     2701 ttgctagcac catatgttga tcaacagctg ctaagctcat ttgatgaaca atatgatcaa
     2761 tttcttcttg tgacttttta gataatgctt ctaatgcttt tttccctttg tcagctagag
     2821 catcaatcat aattgccact tcttgttctt tcgatccacg attttctttt tcaggtatag
     2881 ttaacatata caaccactcc tttatacttt gtgaattatt tcacaaacat tatagtacat
     2941 gtctctcagg atataaagaa aattctatac aaaaaagttt aatttcgaat attatttgaa
     3001 caaatatcaa attttaaaat aaatgttttc atgaaatcat tgttatttcg gtgtttttag
     3061 aatgatttta taatcataat tttttcaatg acataattta ttcataatta tatatttaat
     3121 tctgaatatt ctttaatagc ctaaaaagct tgttatatcg cacaaaacac aacattaaaa
     3181 ggtccttgtc gatacctatc aacagatgtt acaaat
//
LOCUS       DAKTIP010000233         3143 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.238, whole genome shotgun sequence.
ACCESSION   DAKTIP010000233 DAKTIP010000000
VERSION     DAKTIP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.238"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            159..2200
                     /gene="lip1"
                     /locus_tag="O0305_002531"
                     /pseudo
     CDS             159..2200
                     /gene="lip1"
                     /locus_tag="O0305_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
     gene            complement(2647..>3143)
                     /gene="icaC"
                     /locus_tag="O0305_002532"
     CDS             complement(2647..>3143)
                     /gene="icaC"
                     /locus_tag="O0305_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCY0275634.1"
                     /translation="IIFGWIFYFFLGAYMGYNYERVLNFLERYLVIMIVLAVATYFVF
                     IALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQMISAFSFFIYLLH
                     PIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIFRFIIGKQPYKLN
                     INAY"
BASE COUNT         1271 a          478 c          568 g          826 t
ORIGIN      
        1 tatatttttt aaatacaaat gtaaacgctt tctcgtcaaa ttaaacaata gaaaggatgg
       61 tcattatgag tgcttggtta agtaaattat ttgagtttat tcctcgaata attatcaatt
      121 tgtttatcta aaataaaaaa atagaggtgc tgacaatgat gaaaagtcaa aataagtata
      181 gtattcgtaa atttagtgta ggtgcatctt ccattttaat agctacatta ctatttttaa
      241 gtggtggaca agcacaagca gctgagaagc aagtgaatat gggaaattca caggaggata
      301 cagttacagc acaatctatt ggggatcaac aaactaggga aaatgctaat tatcaacgtg
      361 aaaacggtgt tgacgaacag caacatactg aaaatttaac taagaacttg cataatgata
      421 aaacaatatc agaagaaaat catcgtaaaa cagatgattt gaataaagat caactaaagg
      481 atgataaaaa atcatcgctt aataataaaa atattcaacg tgatacaaca aaaataacaa
      541 tgctaatcct agcgatgtaa atcaagggtt agaacaggct attaatgatg gtaaacaaag
      601 taaagtggcg tcacagcaac agtcaaaaga ggcagataat agtcaagatt caaacgctaa
      661 taacaatcta ccttcacaaa gtcgaataaa ggaagcacca tcattaaata agttagatca
      721 aacaagtcaa cgagaaattg ttaatgagac agaaatagag aaagtacaac cacaacaaaa
      781 taatcaagcg aatgataaaa ttactaacta caattttaac aatgaacaag aagtgaaacc
      841 tcaaaaagac gaaaaaacac tatcagtttc agatttaaaa aacaatcaaa aatcaccagt
      901 agaaccaaca aaggacaatg acaagaaaaa tggattaaat ttattaaaaa gtagtgcagt
      961 agcaacgtta ccaaacaaag ggacaaagga acttactgca aaagcgaaag atgatcaaac
     1021 gaataaagtt gccaaacaag ggcagtataa aaatcaggat cctatcgttt tagtgcatgg
     1081 tttcaatggg tttacagatg atattaatcc ttcagtgtta gctcattatt ggggcggtaa
     1141 taaaatgaac attcgccaag atttagaaga aaatggttac aaagcttatg aagcaagtat
     1201 aagtgctttt ggaagtaact atgaccgcgc agttgaactt tattattata tcaaaggcgg
     1261 tcgtgtagat tatggtgcag cacatgcagc aaaatatgga catgaacgtt atggaaaaac
     1321 atacgaagga atttacaaag actggaaacc aggacagaag gtacacctag ttggacatag
     1381 tatgggcggt caaacgatac gtcaactaga agaattactg cgtaatggta atcgtgaaga
     1441 aatagagtat caaaagaaac atggtggcga aatttctcca ctattcaaag gtaatcatga
     1501 caatatgatt tcatcaatta ctactttagg aacaccacat aatggtacac acgcatcaga
     1561 tttagctggt aatgaagctt tagtgagaca aatcgtattt gatatcggta aaatgtttgg
     1621 taataaaaat tcaagagtag acttcgggtt ggctcaatgg ggtctaaaac agaagccaaa
     1681 tgaatcatat attgattatg tcaaacgcgt taaacaatct aatttatgga aatcaaaaga
     1741 taatggattt tacgatctga cgcgtgaggg tgcaacagat ttaaatcgta aaacgtcgtt
     1801 gaaccctaac attgtgtata aaacatacac tggtgaagca acgcacaaag cattaaatag
     1861 cgatagacaa aaagcagact taaatatgtt tttcccattt gtgattactg gtaacttaat
     1921 cggtaaagct actgaaaaag aatggcgaga aaacgatggt ttagtatccg ttatttcttc
     1981 tcaacatcca tttaatcaag cttatacaaa agcgacagat aaaattcaaa aaggcatttg
     2041 gcaagtgacg cctacaaaac atgattggga tcatgttgac tttgtaggac aagacagttc
     2101 tgatacagtg cgcacaagag aagaattaca agatttttgg catcatttag cagacgattt
     2161 agtgaaaact gaaaagctga ctgatactaa gcaagcataa tttataaagt aaagggagga
     2221 attaataatg actgcagact tctttcaatt aatcggatca ttatttagaa ttctaaaaga
     2281 attattcaag taaaacattg gcgaggcccc aacataaaga atttcgaaaa gaaattctac
     2341 aaacaatgca agttggcggg gccccaacaa agaagctggc ggaaagtcag cttacaataa
     2401 tgtgcaagtt ggcggggccc caacatagaa gctggcggaa agtcaggtta caataatgtg
     2461 caagttgggg tgggacgacg aaataaattt tgcgaaaata tcatttctgt cccactccca
     2521 ttggcattta cgaagtttaa atgtgcaatt agaatatatg tataacaata ttaaacacgc
     2581 ggtaaaacga agtcagtcaa ttcaaactga tttcgcccac cgcgtgtttt taacatagct
     2641 taataattaa taagcattaa tgttcaattt atatggttgt tttccaataa taaacctaaa
     2701 gatatagaat tcacgcaata tcatgccgac acctatacat aatcctaaaa tgaatagtag
     2761 tgatatcgct agaaagacca ttgtattatc ctcaaatata tttgtatatg caaacaatga
     2821 gtctagaatg attggatgta ataaataaat aaagaatgag aaagcactaa tcatttgaat
     2881 cgtattaaat aacattgttt taaaatgcgt gcaaataccc aagataacaa taaacataat
     2941 actattatat ggtgttaatg aatatgaaaa gctggtaacg ttccaatagt ctccatttgc
     3001 taacgcaata aacacaaaat aagtagctac agctaataca atcataataa ctaaataacg
     3061 ttctaagaaa tttaatacac gttcgtagtt ataacccata tatgcaccta agaagaaata
     3121 aaaaatccat ccgaatatta tag
//
LOCUS       DAKTIP010000234         3138 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.239, whole genome shotgun sequence.
ACCESSION   DAKTIP010000234 DAKTIP010000000
VERSION     DAKTIP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.239"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            205..2085
                     /locus_tag="O0305_002533"
     CDS             205..2085
                     /locus_tag="O0305_002533"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCY0275635.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDVLLENLGAERICKRVDCDIDYEED
                     AEKWMADIINIIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLIFMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESKRF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            2148..2753
                     /locus_tag="O0305_002534"
     CDS             2148..2753
                     /locus_tag="O0305_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCY0275636.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVVASRRAQTLNQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKKALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            2829..2924
                     /locus_tag="O0305_002535"
     CDS             2829..2924
                     /locus_tag="O0305_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275637.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
BASE COUNT         1161 a          471 c          600 g          906 t
ORIGIN      
        1 taactgacac gacataatca aagttttgtc tgaattactg ataatgtcat tcaagcaaca
       61 tgaaggttgt aaatatttgc aagtataact atcagacaca actcaagttt gttttaattt
      121 ataccataca tattaaaaaa ttgactaaac tatcgataaa aaaagagtca atcatctatt
      181 tcaagtttat tgaggtgaaa acatttgaaa ttaaatacat ctaatagtcc atttacagaa
      241 aaacaagtca cagagattaa caatctgctg caaacattaa cagagagcca acaacagtgg
      301 cttagtggtt ttttactagc taatagtaac gatacgacaa gtgatagtaa tcaacaacaa
      361 ttagagacag aagtgtggca acaatcacaa atatcagaag aacaagcaac ttcaacaacg
      421 tatatgttac aaaataaaga gccacatatc gaagctaatc agcggcatgt tacagtgcta
      481 tatggttctg aatcaggtaa tgccatgcgt ttagctgaaa ttttttcaga acgtttaagt
      541 gatatcggac atcaagttgt tttgatgtca atggatgaat atgatacgac aaacatcgcg
      601 cagttagaag atttatttat tattacgtct actcatggtg aaggagaacc gcctgataat
      661 gcatgggatt tctttgaatt tttagaagac gataacgcac ctaatttaaa tcatgtgaga
      721 tattcagtac tagctttagg tgatcaaaca tatgaatttt tctgtcaagc cggtaaagat
      781 gtagatgttt tactagaaaa tctaggcgct gagcgtatat gtaagcgtgt agattgtgat
      841 attgattatg aagaagacgc agaaaagtgg atggcagaca tcattaatat tattgatacc
      901 acatcagaag gtattcaaag tgaatcggtg ataagtgaat caattaagtc tgccaaagaa
      961 aagaaatatt ctaaatcaaa tccataccaa gcagaagtat tagcgaatat caatttaaat
     1021 ggtaccgatt caaataaaga aacacgacat atagaatttt tacttgatga ttttagtgaa
     1081 tcatatgaac caggagattg tatagtagca ttaccgcaaa acgaccctga attggttgaa
     1141 aaactaatat tcatgttagg ttgggatccg caatctccgg tgccaattaa tgatcatggt
     1201 gatacagttc ctattgttga agcactaaca tcacattttg aatttactaa attaacattg
     1261 ccattattga aaaatgcaga tatctatttt gacaatgaag aattatctga acgtattcaa
     1321 gatgagtcat gggcgcgtga atatgttata aatcgggact ttatagattt aataacagat
     1381 tttccaacta tagaattaca acctgagaat atgtatcaaa tccttagaaa attaccacca
     1441 agagagtatt cgatttctag tagttttatg gcaacgccag atgaagtgca tattaccgtt
     1501 ggtacggttc gttatcaagc acatggacgt gagagaaaag gtgtatgctc ggttcatttt
     1561 gctgagcgaa ttaaaccagg cgatatagta ccaatttatt tgaagaaaaa tccgaacttc
     1621 aaatttccga tgaagcaaga tataccggtt attatgattg gaccaggtac tggaattgct
     1681 ccttttagag catatttaca agaacgtgaa gaacttggta tgactggaaa aacatggttg
     1741 ttctttggtg atcaacaccg tagttctgac tttttatatg aagaagaaat agaagaatgg
     1801 cttgaaaatg gaaacttaac acgcgtagat ttagcatttt caagagacca agaacacaaa
     1861 gaatatgtac agcatcgtat aatggaagaa agtaaacgtt tcaatgaatg gattgagcaa
     1921 ggcgcagcaa tctatatttg tggcgatgaa aaatgtatgg cgaaagatgt ccatcaagcc
     1981 attaaagatg tattggtaaa agaacgtcat atttctcaag aagaagcaga gttattattg
     2041 cgacaaatga aacaacaaca acgctatcaa cgtgatgttt attagcgatt ggtgttaaat
     2101 attttaaggt gtaatgatgt aaaaagatat aaaggatgtt gctcaacatg aatatgccat
     2161 taatgataga tttaacaaat aaaaatgtcg tcatagttgg tggaggcgtc gttgcaagtc
     2221 gtcgggcaca aacattaaat caatacgttg aacatatgac ggtcatcagt ccgacaatca
     2281 ctgaaaaact tcaaaatatg gtagataacg gtgtcgtcat atggaaagaa aaagaatttg
     2341 aaccaagcga tattgtagac gcgtatctag ttattgcagc aaccaatgag ccacgtgtca
     2401 atgaagcggt aaaaaaagcc ttacctgagc atgccctttt taataatgtt ggagatgcat
     2461 caaatggcaa tgttgtattt ccaagtgcac tacaccgcga caagctaact atcagtgtat
     2521 caactgatgg tgcgagtcct aagttgacaa aatcaattat ggcagagctt gaggcgttat
     2581 atccaccatc atacagttcg tatatcgact ttttatatac ttgccgacag aaaataaaag
     2641 tacttgatat aacatataac gaaaagcaac agttactgtc acaaattgtg tcacaagaat
     2701 atttaaatca tgacaaacaa gctcaatttt tagcgtggtt ggatgtaaga taataatagc
     2761 ggaccgtcta accgtctaag gtaagtcttc ttattttaac tttaacgctt aatcattgaa
     2821 attaagacat gggcggcttt gtgaatagtc taataatgaa ggatttaagc gataatgata
     2881 tgcgttttaa atatgaatat tacaatagag aaaaagatac gtagaacaaa cttaataaaa
     2941 taggtggata aattgaaatc tggttgaagt cgttactatc atagcgacct ttagccagat
     3001 tttttgtgca atagaaagca ataataaaaa tgatagatca aaatgaaata caggacagga
     3061 tatacaagga ttagtcatgc catgttatca agtaggaaaa tcaaacttca ctattgatag
     3121 ttacgcaaaa aagatttt
//
LOCUS       DAKTIP010000235         3125 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.240, whole genome shotgun sequence.
ACCESSION   DAKTIP010000235 DAKTIP010000000
VERSION     DAKTIP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.240"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(55..243)
                     /locus_tag="O0305_002536"
     CDS             complement(55..243)
                     /locus_tag="O0305_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275638.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKVMNFKKNN"
     gene            complement(826..1821)
                     /locus_tag="O0305_002537"
     CDS             complement(826..1821)
                     /locus_tag="O0305_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCY0275639.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADIL
                     EILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQK
                     VIEKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFM
                     RSTEVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVAN
                     VKAIKPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSY
                     GGLLKQKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     gene            2273..2854
                     /locus_tag="O0305_002538"
     CDS             2273..2854
                     /locus_tag="O0305_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275640.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIA
                     DKNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDI
                     ENKKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVA
                     KKLAKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
BASE COUNT         1139 a          519 c          379 g         1088 t
ORIGIN      
        1 aaaaaaacag tgtgaaaaca agaagaaact cgtttcaaaa tcccctcaaa tattctaatt
       61 attctttttg aaattcataa ccttaacaac caaataaata gcatttattc ccatcaaaat
      121 aacatacgga attaatatat ataaattttc caaatgagat ataaaaatta atccaagtaa
      181 aaagaaaaca tttaaaatag ttgctataac gtaaccttca tctaacactg tgtttttatt
      241 caaaataaac actccttttt taaaaaactt tatcacaaga cttactcaaa ttataaatac
      301 ctttattttg agttttaagg gggtatttta aaggtttagg gggtatcata taggttttta
      361 acttaaaaat atatacaagc tcttaaaacg caaatatgag ccaaataaat atatggtaat
      421 ttcacaaaca aaaatttcag caaaaccagt gactaatttt ttaacactgc ccatttacat
      481 gcaaattaaa aattagcata aaatccttta aaacttccac atgtttttct aaattcctgc
      541 agggcgtttt ttatcaaaaa tcgagaattt ttaatttttc tctaaaaact ccgtaagagc
      601 ataaggagaa tttgctatca ttcaaaaatg ataggtctgg caaagccaga aattccaggg
      661 gttttaaggt attttaaagt gatatcatac aaagctgtac tgtcttattt ttgtgacaca
      721 atatattgcg tttgtcttat ttttgtgaca ctacatacag catttgtcac aaaaataaga
      781 cagtactttt ttataaaaaa tcaacaaaaa agacattagt caacactaat gtcttaaata
      841 ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt cttcttcgtc
      901 tgttgtttta tcttcatcac ttgcattaat caaattacca tcttcgacat cgtctaagtt
      961 taaaatttta tgtttttgtt taagcaatcc accataactt aacatacgtt ttcgatacaa
     1021 acctttttct aaatcactta caatttcttg atttttttca tcatcatcag ttaaaaaatc
     1081 agatgattta accgaatatt ttgaggtctc tttgattgcc gattcaatgt ctttatcgcc
     1141 ttttctattc ggtttgatcg ctttaacatt agcaacaggt cgatagtcaa cttgtaatgc
     1201 tctttgccat aaattaaccc attcttcttg agttatataa ttctcttttt ttctaaaata
     1261 tgcattttca acacataaca aaacatgcat gtgctgatta taactaccgt catttttatt
     1321 aacggtaact tctgttgaac gcataaatcc aacaagattt tgtttaacct ttttatatct
     1381 actcaaccta tcaaatgctt tagttagatg cttcaaactt tgttctaaag tatctccatc
     1441 tatcgcattt tttgttgaaa gtgttaaaaa caaccaacgt gcttttggct tttccttaat
     1501 tactttttca atcacttttt gagcttgata actatttttc atagcacgtc tccaattaca
     1561 aaccggacat aattttgatt tacaaaacca tgtcttatgt aacttcaaat agccttcatc
     1621 agttggctta aattctaaaa tattaccaca ttgttttaca ttaaaagcct ttttgatttt
     1681 taaaatttct agtatatctg catagcttac attatctatt ttcttttctc tccatgggcg
     1741 ttgtttccca cttttcgtca tatcttttaa cgttttatta tcttgatttt cggttatact
     1801 tctagtagta ttatattgca tataaaaaga cccccagtat tatttaagat tcgacacctt
     1861 aataatataa ctggggtttt ttattgtcaa attttccttt cacgcacaaa aacacgttta
     1921 aacactgtaa tatcaacagt tacagctata tatataaaat cgagttattt ctatatagta
     1981 tcaagacaag aagaaactcg ttcacactcg tttcaaaaaa caacctattc cttcttcttt
     2041 ttatgaccct ctaaacctaa ataaattaat gtgaaaacca atgaataaat aaaactttct
     2101 aaaaagtcaa aaggattatc aaataaaatc gaattaacaa aattaaaaat taaaaataca
     2161 gataataaaa taatcaaaaa gtaaaaaaac ttattcatat taaactcctt tgtttttata
     2221 tgttaatatt atattgaatt catttaaata acaaaacaag ggggcattta caatgaatgc
     2281 aattcaaaac agtatatcac taagaaagat tttagtagta tctttaagta ttttaaccat
     2341 actttcaatg gtcttggatt ttaatttcaa agaagctcaa gcccaaaaca aaaataacat
     2401 tgcagataaa aacgttgaaa ctttaaacga aaaagaaatt gaaaaagaac taaaatacat
     2461 atatggcaaa attattattt tagataaaga cggaacagct aaagatgtta atctagaaaa
     2521 tgccaaatct cgatatggat atgttcctga aggattccaa aaactaaaaa acgacataga
     2581 gaataaaaag aaaactagtt caatatcacc tagagcagtt ggtgggaact acaaaaatag
     2641 cggtgactgt ttctattctg aagttttaaa ctcatatgga gaacttttaa ctggaaacat
     2701 aatcacagca gtttttgaag atgttaaagc caaaaactta aagggcgtag caaaaaaatt
     2761 agctaaaatt gggataaaag gaaacctagc tggaatcgca gctactatgg tttcgaagga
     2821 tgtacaatgt aatcttaaat acgggttact ttagatacta tttattccaa ttgctttatt
     2881 gacgttgagc ctcggaaccc ttaacaatcc caaaacttgt cgaatggtcg gcttaatagc
     2941 tcacgctatg ccgacattcg tctgcaagtt tagttaaggg ttcttctcaa catcaataaa
     3001 ttttctcggc ataaatgcgt cgtctatttt ttattatctt catgataatc atatttttct
     3061 tccatctcgt caaaagcatc atctttatag aaaaacaaaa agtaagcaac aacaccaata
     3121 ggaat
//
LOCUS       DAKTIP010000236         3119 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.241, whole genome shotgun sequence.
ACCESSION   DAKTIP010000236 DAKTIP010000000
VERSION     DAKTIP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.241"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..974)
                     /locus_tag="O0305_002539"
     CDS             complement(<1..974)
                     /locus_tag="O0305_002539"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCY0275641.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEETDQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCG"
     gene            complement(987..1970)
                     /gene="mvaD"
                     /locus_tag="O0305_002540"
     CDS             complement(987..1970)
                     /gene="mvaD"
                     /locus_tag="O0305_002540"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCY0275642.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            complement(1975..2895)
                     /gene="mvk"
                     /locus_tag="O0305_002541"
     CDS             complement(1975..2895)
                     /gene="mvk"
                     /locus_tag="O0305_002541"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCY0275643.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
BASE COUNT          907 a          673 c          439 g         1100 t
ORIGIN      
        1 ccacagtctc caccaccagc gcctgatgtt ttagatgcgc cgtgatactt ttcagcaata
       61 tcacacaaat attttagctt ttcagtttct atatcaactg tagcttcttt atccatacgt
      121 tgaataattg tacgattctg acgcaccatc ttttgcacac ctttaatgtt atttgtttta
      181 aaagcatgaa taagtttttc aacacaacga tgtgaatctt ctaagaagtc accgtaaaat
      241 gaaggatctg atttcaaacg tttcacttcg ctaacaaagt gtggtgatga cgccggtgag
      301 ccagtccaac cgataagtac ttccatattt tcaggtgctt gtaatggttc gatgtgcaat
      361 ccaggccagt ttttgattaa aacttcttca accgtagtat cttcaatttg atgcttaacc
      421 cattcatgat caaaagtact atacgctagc catccactat atacactcac agcaatatct
      481 ccgcatgaac ttaaactttg taacttcata tttgcaatca ctgctagttt ataaatgtat
      541 aaattagata acttcatatc ataaaattca tttaatactt ttataactga cacaagtact
      601 gctgcacttg aacctaatcc atatttatga ccatttgaat catctaaatt actatcaata
      661 gtcagatgaa aatgcttcat cgctatatcg caacttttcg cgtattgttc aaatatttca
      721 atagctgtga ccacataatt taattgtttt gctgcatgtg gatctgaaat gacaatacta
      781 tcttcatctc tactaaatgt aactgggtta tgatgtaatg cttttgaatg aatggtacct
      841 ttatattggt ctgtttcttc aatagtagca gttacaaaac gatctaacgc aataagtaca
      901 gatttatatc ctggttctgt tacagcatat tctccagcaa tataaagttt tccgggtgct
      961 ttgacctgaa tcattttatc tcttccttac tcaattattt caattcctgt ggcaataatg
     1021 tcactatcaa taatttggtt attatcaaac tgtgttaata atttatctat aatctgttgc
     1081 ttgtttttct tttctacaag tattttcaca ttaggtcccg catccattgt aaaataacac
     1141 ggataccccg cttctcggca ttcgtgaaca agcgccatga catcataact ttcttgcaca
     1201 agatatgtga acggcggtgt tgatcctaga ttcgtggcat gcatacgcaa accattttct
     1261 tcaattactt caccaaggcg tttaaaatct ttgtcttgaa tcgctgcttt tgcttcagct
     1321 aaatcttcat caatatgatc taaccaatat tgataaaacc ttgatgtgtt tcgtgtcaat
     1381 gacataccat atcgactagg taccttttta gaatgttgat taatcacaac aaatatcatg
     1441 gcaaggtcat cttcaaaatg attcgattca agtggaacgg catatgacgt ctcatcacta
     1501 tacccttttt cccattctgc aaatccacca taaatactac gcgacgcaga acccgaacca
     1561 attcgcgcca atctcgataa atccttatct gacagctgca tgtctagcgc ttgattacaa
     1621 gctgctgcta aagctgcata tgcgcttgcc gatgaagcca accctgctgc tgttggtaca
     1681 aaattgtcgc tttcaatttc tgcataccaa tcgatgccag ctctatttct gacaatatcc
     1741 atatattttg aaattttctc taattctttg ccactaacct tttcaccatt caaccaaaat
     1801 tgatcctgtg ttaactggtc gttaaaagtg actttcgttt cagtgtaaaa tttttctaat
     1861 gtaacagata tgctattatt cattggaatg attagtgctt catctttttt accccaatat
     1921 tttataagtg caatattcgt atgtgcacgt gctttgccac ttttaatcaa cgcattaacc
     1981 tcctaaattc tcaatccaag tatgtgctgc accagctttt tctacagctt ttacaatatt
     2041 tttcgctgtt ggtaaatctt tggcaagcaa taacatactt ccaccacgac cagcgccagt
     2101 aagttttcca gcaatcgcac cattttcttt accaattttc attaattgtt ctattttatc
     2161 atgactaact gtcaacgcct ttaaatccgc atgacattca ttaaaaatat ccgctaaggc
     2221 ttcaaagtta tgatgttcaa tcacatcact cgcacgtaaa actaacttac cgatatgttt
     2281 tacatgtgac atgtactgag ggtcctcaca aagtttatga acatcttcta ctgcttgtct
     2341 tgttgaacct ttcacaccag tatctataac aaccatatag ccgtctaaac ttaacgtttt
     2401 caacgtttca gcatgacctt tttggaacca aactggtttg cctgatacaa tcgtttgcgt
     2461 atcaatacca cttggtttac catgtgcaat ttgctctgcc caattagcct tttcaatgag
     2521 ttcttctttc gttaatgatt tccctaaaaa atcataactt gcacgaacaa aagcaaccgc
     2581 gacagctgca ctcgatccta atccacgtga tggtggtaaa ttcgtttgga tcgttactgc
     2641 tagcggctct gtaatattat ttaattctac aaaacggttc accaaagact taagatggtc
     2701 aggcgcatca tataacatac catcgtaaac atcgctttta atagacgaat agttcccgct
     2761 ctctaaggct tctattaaaa ctttgatttt acctgcgtta aacggtactg caatagcagg
     2821 ctctccaaat gtaacagcat gttctcctat taaaataatc ttacctgtcg attccccata
     2881 tccttttctt gtcatgtcaa tatcaccttt tatatttatc ctatacttga ttcattattt
     2941 ttatttatta gtaaaagaca tcatattcta agttgcatac gcattctcgt taaatttcat
     3001 tgcagtcttt atctcacatt attcatatta tgtataatct ttattttgaa tttatatttg
     3061 acttaacttg attagtataa aactaacttt cgtttacttc aaagtttaaa tcttatcga
//
LOCUS       DAKTIP010000237         3118 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.242, whole genome shotgun sequence.
ACCESSION   DAKTIP010000237 DAKTIP010000000
VERSION     DAKTIP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.242"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..283
                     /locus_tag="O0305_002542"
     CDS             <1..283
                     /locus_tag="O0305_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275644.1"
                     /translation="VIKLQLNLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITAILY
                     YTLDGFYASLTGVLGTLVALNPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            479..1864
                     /gene="fumC"
                     /locus_tag="O0305_002543"
     CDS             479..1864
                     /gene="fumC"
                     /locus_tag="O0305_002543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCY0275645.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(2327..>3118)
                     /locus_tag="O0305_002544"
     CDS             complement(2327..>3118)
                     /locus_tag="O0305_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCY0275646.1"
                     /translation="DLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPARLMDKVHTGD
                     DVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKESNTLMNHVIYT
                     IDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKANVKALLPLKPQ
                     TIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDTGRTHQIRVHL
                     AEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
BASE COUNT         1103 a          456 c          545 g         1014 t
ORIGIN      
        1 agtaataaaa ttacaactca atttatcaaa aggattaatt gatgatgtta atgcgcaaac
       61 attcattaaa agtaatcaat caggtattgt aatcagtgta atttgttgtt tattaataac
      121 agcaatattg tattatacac ttgatgggtt ttatgcgagt ttaacaggag ttttaggtac
      181 gcttgttgct ctaaatccat atacgacaat tgttagtgct gtattatata tacttacagc
      241 tattgctacg gttgaagcta ctaaaacaaa gtatcgtaat taaacttagt tcaataataa
      301 ctaaataatg atagataaca cactaatcgt atgaccgtat cactgtaata aagggtgaac
      361 acgtgcgatt agtgtatttt tatttcaaat tatgaaaacg ttttaatcat aaattgctca
      421 attaaattat ttaatgatat actacagtta aataaacagt gataagggga gaaattgaat
      481 gtcagtaaga attgaacatg atacttttgg agaaatagaa gtacctgcag ataaatattg
      541 gggtgctcaa acagaaagaa gtaaacgtaa tttcccagtt ggtaaagagc gtatgccaat
      601 cgaagtagtt tatggttttg cacaactaaa gcgtgcagca gcaatagcta attttgattt
      661 aggaaaatta agcgaggcaa agaaagatgc cattgtatac gcatgtgatc aaattttatc
      721 aggtgaatta gatgaacact tcccactagt tgtatggcaa acaggaagcg gtacacaaag
      781 taatatgaat gtgaacgaag tagtaagtta tgttgctaat atgtatttaa aagatcatca
      841 aagtgatgaa agtatccacc caaatgatga tgtaaataaa tctcaaagtt cgaatgatac
      901 attcccaact gctatgcacg ttgcattata tcaagaggtt gaaacaaaat tagaacctgc
      961 attaaaactt ttaagaaata ctttgaaaga aaaagaagat aaatttgatt caattattaa
     1021 aattggtcgt acacatttac aagatgcaac gccgatcaaa ctaggacaag agattagtgg
     1081 ctggcgttat atgcttgacc gttgtgaaac aatgttatct gaatctaaga agcacatttt
     1141 aaatcttgcc atcggtggta cggctgttgg tactggtatt aatgcgcatc ctgaatttgg
     1201 tgataaagtg gcacattata tttcagaaaa tacgggttat ccatttgtat cttctgaaaa
     1261 taaattccac gcacttacag cgcatgatga agttgttcaa ttgcatggaa cattgaaggc
     1321 attagcagga gacttaatga aaattgctaa tgatgtgaga tggttggctt cagggccacg
     1381 agctggtttg gcagaaattt ctatccctga aaatgaacca ggttcatcaa ttatgcctgg
     1441 taaagttaat cctacacaat gtgaaatgtt aacaatggtt gcagtccaag taatgggtaa
     1501 tgatacagtt gttggcttcg caagttcaca aggtaacttt gaattgaatg tttataaacc
     1561 agttattatg cataatacac tacaatcaat ttatctttta gctgatggta tggaaacatt
     1621 taataacaat tgtgcagtgg gcattgaacc aatcgaagag aatattgata attatttaaa
     1681 tcaatcatta atgttagtta ctgcattaaa tccacatatt ggttatgaaa aagcagctca
     1741 aattgctaag aaagcccata aagaaggttt aactttaaaa gaatctgcaa ttcaaactgg
     1801 atatgttaca gaagaacaat ttgaagcatg gattaaacca gaagatatgg tagatcctca
     1861 ttaattaaat attaatataa aataaagttt gggcattaca ggtagatgta gtgttcaagc
     1921 tttatttttt gtctatgtca aattgaactt ataagtctga tttgatatag agccatgaaa
     1981 aggaaaaact tttcatggag tccgttttat tatagaacct attttttata tgatgaattg
     2041 ttgtacatgt attggccaaa caacctgtga taaaaatctt atgatgtcat gtattgtcca
     2101 tagatacacg taacgcagtt gaaaaagact acaagtcatt atccaaggat gttataaaat
     2161 gaataattac taaaatatca aaatataaaa tcctaagcat cgtaaaaaac aaaaaaaccg
     2221 aacatctgaa aaaattaata attttccatc tatccgattt aagttgatac ttgatttagt
     2281 tacaatgcgg tattgcatgt ttaaatatca tgcaaaacta atcaaattag tcatccaaag
     2341 aaacggaaat gacttcctta gtcaagggat gcacaaactc tattttgtaa ctttcaagtt
     2401 ctaattgtct taacgttgaa tcaccatata aaggatcacc aataacaggg tgaccaattt
     2461 cagctaaatg cacacgaatt tgatgagtac gtccagtatc caacttgatt tcaagttggc
     2521 acacagcttc ttttatcatt tttgaagtta aaatgtgtgt aattgcacgc tgtccagtag
     2581 gagacacacg tcgtttattc gaatggaatt tatctttacc aattggcata tcaatcgttt
     2641 gtggttttaa aggtaaaagt gccttaacat ttgctttgta tatccgcgta atatcattgt
     2701 cttctaacat gcgatcaaga atttttttca ttaaaggatt tttagcaaca attaataatc
     2761 ctactgtttc ctggtccagt cgatgaattg gttcgacata gtcactatca atagtgtaaa
     2821 tcacatgatt cattaaagta ttgctttctt ttaaatcatt agggtgagtc ttaacacctt
     2881 taggtttcat tacgattgcc atatcatcgt cttcgtattt aatttgtgca taacgataac
     2941 ttggaacata attacttttt tcatcgatgg ttggaacaaa tacatcgtcg ccagtatgca
     3001 ctttatccat taatcgcgca ggtttatcat taatagtaat atctttagac atatttaaat
     3061 gatgtaaatc ttttttaggt accttaagtt gttggaaaat atctcgtaaa cttaagtc
//
LOCUS       DAKTIP010000238         3103 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.243, whole genome shotgun sequence.
ACCESSION   DAKTIP010000238 DAKTIP010000000
VERSION     DAKTIP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.243"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            123..515
                     /locus_tag="O0305_002545"
     CDS             123..515
                     /locus_tag="O0305_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCY0275647.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEKMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            517..>3103
                     /locus_tag="O0305_002546"
     CDS             517..>3103
                     /locus_tag="O0305_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0275648.1"
                     /translation="MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKY
                     EDEFLS"
BASE COUNT         1225 a          326 c          551 g         1001 t
ORIGIN      
        1 atgcatggag tagagcgttc atgtgaatgt aaaatattaa tttttaatat tgatttatat
       61 atagttttgt aatttttgat agaataacaa ttaagatgat tttaaattga agggtgagtc
      121 ttatgaaaaa agtaatcaat gtagtaggag ctattatttt ttctgataac aaaattcttt
      181 gtgcacagag aagtgaaaaa atgagtctgc ctttaatgtg ggaatttcct ggcggtaagg
      241 ttgaaaagaa tgaaactgaa aaagacgctt tgattagaga aattagagaa gaaatgaaat
      301 gtgatttaat tgttggagac aaagttataa ctacagaaca tgaatatgat tttggaattg
      361 ttaggttaac aacatacaaa tgtactttaa acaaagagtt accaactcta actgaacata
      421 agagtattga atggttgtca ataaacgaac ttgataaatt aaattgggcc ccagcggata
      481 taccagccgt taataaaatt atgactgagg gataatatga gtagattact aaatgatttc
      541 aatcaatctt tacataaagg gtttatagac aaacatattt ctcataaagg gaattataca
      601 ccgaagcttt tagtaaataa taaaaatgaa aaagttcttt caacaattat agatgaactt
      661 caaaaatgtg aaacgtttta tttttctgtg gcttttataa cagaaagcgg tttagccagc
      721 ttaaaggctc aactattaga tttaagcaat aagggtgtta aagggaaaat attaacatct
      781 aattacttag ggtttaatag tcctaaaatg tatggagaat tacttaaatt aaaaaatgta
      841 gaggtaagat taactgatat tgctggattc catgccaaag gatatatttt tgagcataag
      901 gattatagtt ctatggtaat aggaagctct aatttaacat ctaatgcgtt aaaggttaat
      961 tatgagcata atgttttact gtctactatg aaaaatggcg atttagttga tagtgtaaaa
     1021 agtgaatttg acttgttatg gcaaaagagt accccactga ctgagcaatg gattaattca
     1081 tataaagaat catttgagta ccgttcctta gaaaaattag cggaggttga acaaactcaa
     1141 atgctgctag ctgataaggt gaaaaaatca gtggaaattg tacccaattt aatgcaagca
     1201 gaagcattaa ggtcattaaa agctattagg gataaagcca aagataaggc gttaataata
     1261 tctgcaactg gtacaggtaa aacgatttta tgtgcattag atgttagaga agtaaaccct
     1321 aataaatttt tatttattgt tcataatgag gggattttaa atagagctaa ggaagaattt
     1381 aaaaaagtat tacctataaa aaatgatagt gattttggat tgttgacagg aaaacataga
     1441 gatgtcgatg ccaaatattt atttgcaacc attcaaacat tatctagaga tgataatttt
     1501 aagcaatttg atgaaaatga atttgattat attgtttttg atgaagcaca tcgttctgct
     1561 gcatctactt atcaacgagt atttaactac ttcaaaccta agttcatgct gggaatgact
     1621 gctacaccag aaagatcaga tgaattaagt atatttgaac tgtttgatta taatattgca
     1681 tatgaaataa ggttacaagc agcattagaa agtgatattt tatgtccatt tcattatttt
     1741 ggtgtgactg attatgtaca tcaaggtatt aaagaagatg atgtaactaa actgagatat
     1801 ttaacttctg atgaaagagt taattatatt attcaaaaga cagattacta tggatattca
     1861 ggtgaaattt tacaaggatt gatttttgta agtagcaaaa aagaagctta tgatttagcg
     1921 gataaactaa gttcaaaagg gattaaaagt gttgctttaa caggagatga tagtgttaat
     1981 tatcgccaaa ttgtaataga aaaattaaaa gaaggtaaga ttaactatat tataactgta
     2041 gatcttttta acgaaggtat tgatattcca gaagtcaatc aagttgtaat gttgagacca
     2101 acagagtcta gtattatatt cattcagcaa cttggtagag gattaagaaa aagttcaaat
     2161 aaagagtatg ttacagtaat tgattttatt ggtaattata agacaaatta tttaattccg
     2221 attgcgcttt ctggggatca atcgcaaaat aaagataatt ataagaagtt tttaacgaat
     2281 aacgattcga ttaatggagt atctacaatt aattttgaag aagttgctaa aaaacagatt
     2341 tacaattcat tggatgcagt atcattaaat caaaataaat taatattaaa agcttatgaa
     2401 gaagttgaaa atagattggg acacatgccg ttactaatgg atttcataca acaacattct
     2461 atagatccaa gcgttatatt ttctaaattt agtaattatt acgagttctt agtgagatat
     2521 aaaaaaatag atacattatt gacggaaaat gaatctaaaa atctggtttt cttttcaagg
     2581 caaatagcac ctggattgaa aagaatcgat agtttagttt tagaagaact tttaaaaaat
     2641 gaattaacat atgatgaatt aaaaaataaa atgttgaacg aagttaagga tataacagaa
     2701 gatgatatag atacttcatt aagaatttta gatttttcat tttacaacgc aggaattgaa
     2761 aaaatctatg gaagtcccat tatagaacgt aatgaaagaa tgataagact aagcgatgca
     2821 tttactaatg ctctaagtaa tcaaacattt aatatgtttt tagaagatct tattgaatta
     2881 tcaaagtata acaatgaaaa ataccaaaaa ggtaaaaatg gactgatttt atataataaa
     2941 tattcaagag aagattttag taaaatattt aattggaata aaaatggttc gtctgtaatc
     3001 atgggataca tgataaaaag ccaagaaatg ccaatattta tcacttatga taagcatgag
     3061 gatataagtg atagcacaaa atatgaagat gaatttttaa gtc
//
LOCUS       DAKTIP010000239         3070 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.244, whole genome shotgun sequence.
ACCESSION   DAKTIP010000239 DAKTIP010000000
VERSION     DAKTIP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.244"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(87..1223)
                     /locus_tag="O0305_002547"
     CDS             complement(87..1223)
                     /locus_tag="O0305_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCY0275649.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(1255..1884)
                     /locus_tag="O0305_002548"
     CDS             complement(1255..1884)
                     /locus_tag="O0305_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCY0275650.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(1903..2172)
                     /locus_tag="O0305_002549"
     CDS             complement(1903..2172)
                     /locus_tag="O0305_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCY0275651.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            2335..2643
                     /locus_tag="O0305_002550"
     CDS             2335..2643
                     /locus_tag="O0305_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCY0275652.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            2814..3014
                     /gene="cspA"
                     /locus_tag="O0305_002551"
     CDS             2814..3014
                     /gene="cspA"
                     /locus_tag="O0305_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCY0275653.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          942 a          465 c          493 g         1170 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaactttaa ggaacttaat gtcccaagct
       61 cttttttgtt atatctaatt cgtaatttat gattgtttat tcggtccttt gatgttcact
      121 aaatgtgact ttaaatcttg ttctaattgt tgtaattctt tttcagctaa ttgtcgttct
      181 tcgcgaccgt gttgttgaat aattaatgtt tcttcaattg tctcaataat gttacgctgt
      241 gtacgtttca atgtatcaag atcaacaatg ccacgctcat tttctgttgc agtttcaatc
      301 gcattttgtt tcaacatttc agcatttgct gttaataaat cattagttgt atcagtgaca
      361 gctcgttgtg cagcaactgc attacgctgt ctcattaatg taagcgcaat ggccatttga
      421 tttttccata gtggaatact tgtcaaaatt gaactttgta tcttctcggc aagtgcttga
      481 ttaacatttt gaatcatacg aatttgtggc gcagtttgca aagctatttg tcttgaaagc
      541 tgtaagtcat atatgcgttt atctagtcta tctataaatt gctgcatatc tgcaacttgt
      601 tgaatatcca tttgattagt ggattgctgc gcttgctgtt gcaattgtgg tagcttttca
      661 ttttctaatt gcaacttttt ttgctgtgca gcaatgatat gcaatgataa gtcatcaaag
      721 tattgtttgt ttttatcata tagcgtatct aataattcaa tatctcttgt taaatgtgtt
      781 tgatgtttct gcagttgaat cgttatgcga tcgacttgag cactaactga ttgcattctt
      841 gaaaagattt cattgataga cgactttgct ctgctaaaaa ttctttttaa catagatggt
      901 ttatcagtat ttaactcatt tggattaact gactttagtt ttgacattag atctgacaaa
      961 gtatctccaa taggaccaac atctttactt tgtacttcat ccaacatttg atgtgaaaat
     1021 tgagacattt gtttctgtaa atcagaacca aacgctaata aaccttcatt gtctaaaggg
     1081 ttaatttgtt tactgattgt gtctacctgt ttttgttgtt caattgttaa cttttctttt
     1141 tgaatagtag tattcgataa ttctttatcg cttataaaat catctagtgg atggctttct
     1201 ttgaaacttt tattttcagt catcaatcat ctctccaata gttgctattt tacatcaatc
     1261 ttgatgatgt ttttgatgta acttatttaa ttccatttca atatctaaac gttcataatc
     1321 atcttcgttg agacgcttta aatcagcgat taatgttcgt ttgacctcat ccaaagtaat
     1381 tcgtgtttgt tctaactttt gctgttcatt aattgatttt ttgggcattt ttgctagacg
     1441 tgtatatgca tcaaccaaat ttaaagcatt atcaatatga gaataaaaaa agccttcaac
     1501 tttataaaat gatgcaggtc tctgtctaac tgtcgtataa atagaacgtg aaatttggta
     1561 tatatcatta atctgcctaa aatctttaat tgatcttata ttgacatacg tttttaaaat
     1621 acctctaagt ttttggtgtg tatgatttaa ctgatttcga atatagcgat agtcttttct
     1681 agtcaaacca atttcgttta aatatttgcg tgaagtgagt ttttgtatcg gtaggtatgt
     1741 cattaaaaag ccaacaatac caatagacat atcaattaaa aaagatacat caagtgcaat
     1801 catcccaaat atgcttgtta aaaacgctac aggaattccc actaacaccc caaatatatg
     1861 agaaatatta tatctcactg tcatcttcct ttatttagca ttttatattg atcgaaaatc
     1921 cgataatttt tgatttagtt ctaactcttc aagttgatgg cttgttacat ttgatgctgg
     1981 tgaggcacct tcaattacac cttgaataaa tctctctata tctgcgtcat ccccttgtgc
     2041 atatatctct acatagtcat ctacattttg aacagtaccg acaatgttat agttcattgc
     2101 aatgcgttgt gtaaaatatc taaatccgac gccttgaacg cgtccgaata cttgtaaatg
     2161 tatatgtctc atttttacca cctcataatg ttattatacg tagttttact taaaaaaact
     2221 aataattact atagttacta ctttgtttgt ttcaagtcgt caaacttgat tttcagagga
     2281 taaaggtata aaaataagta tagagttttt gaagtatgga aggggtcttt aataatgtgg
     2341 acagttacca aaattagagc cgattatgag ggatggtggt tattcagtga ctggccagaa
     2401 aacattgttg aaaaatatca atatcaagat tttgatgaca tgtttaagca ctatcaacaa
     2461 ttgattaatc aatgtaaagt tcagttcgat aactatgtca caggcaaata taatatttat
     2521 gcattttata ataattgtga catgaactat tgcgaagatt gtgaagagga tttacaaata
     2581 ttttatagtt tcattgtttt acaaaataat gaagtatatt ataaactacc aataattgat
     2641 taaatgaata ttttttatta ttgtattgca atttggtaat aactgcacta taatacaagt
     2701 taagcagatg attattccat attgcaaaga atatttgagt gaaatttgca tgtatttttt
     2761 gtaatatgcg aataagcata ttgaatgaat tttagtcttg gaggtttcag attatgaaac
     2821 aaggtacagt taaatggttt aacgctgaaa aaggattcgg ctttatcgaa gttgaaggag
     2881 aaaatgacgt attcgtacat ttttcagcaa ttaaccaaga tggttacaaa tcattagaag
     2941 aaggtcaagc tgttgagttt gaagtagttg aaggcgaccg cggtccacaa gctgcaaacg
     3001 ttgttaaact ataattctta gatttgaatc attgatttta actaacacct taccaagaag
     3061 taaggtgttt
//
LOCUS       DAKTIP010000240         3050 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.245, whole genome shotgun sequence.
ACCESSION   DAKTIP010000240 DAKTIP010000000
VERSION     DAKTIP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.245"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..114)
                     /locus_tag="O0305_002552"
     CDS             complement(<1..114)
                     /locus_tag="O0305_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275654.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLY"
     gene            278..1516
                     /gene="pgaC"
                     /locus_tag="O0305_002553"
     CDS             278..1516
                     /gene="pgaC"
                     /locus_tag="O0305_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCY0275655.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            1480..1785
                     /gene="icaD"
                     /locus_tag="O0305_002554"
     CDS             1480..1785
                     /gene="icaD"
                     /locus_tag="O0305_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCY0275656.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            1782..2654
                     /gene="icaB"
                     /locus_tag="O0305_002555"
     CDS             1782..2654
                     /gene="icaB"
                     /locus_tag="O0305_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCY0275657.1"
                     /translation="MKYRKFIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            2641..>3050
                     /locus_tag="O0305_002556"
     CDS             2641..>3050
                     /locus_tag="O0305_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0275658.1"
                     /translation="MKKIRLELVYLRAIICAIIIITHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSY"
BASE COUNT         1076 a          448 c          497 g         1029 t
ORIGIN      
        1 atataaactc gctttcttta tatttacact tttagctata tcatcaagtg ttgtaccgtc
       61 ataccccttc tctgaaaata aggttattgc gttatcaata atcttatcct tcaattttta
      121 taacccccta ctgaaaatta atcacactat gttacaggaa aattaagttg caattacaaa
      181 tatttccgtt taattataac aacaatctat tgcaaattaa aatactatca attaccatat
      241 ggcttacaac ctaactaacg aaaggtaggt aaagaaattg caatttttta actttttgct
      301 tttttatcct gtatttatgt ctatttactg gattgtcggt tcaatttatt tctattttac
      361 cagagaaatt agatattcat tgaacaagaa gcctgacata aatgtggatg aattagaagg
      421 cattacattt ttacttgcct gttataacga aagtgaaacg attgaagata cgttgtctaa
      481 tgttcttgca ctcaaatacg agaagaaaga aattattatc attaatgatg gaagttcaga
      541 taatacagca gaactcatct ataaaatcaa agaaaataat gactttattt tcgtcgattt
      601 acaagaaaac agaggtaaag ccaacgcact caatcaaggc attaaacagg cttcatatga
      661 ttatgtaatg tgcttggatg cagatactat cgttgatcaa gatgcaccat attatatgat
      721 tgagaatttc aaacatgatc caaaacttgg tgcagttaca ggtaatccta gaattcgaaa
      781 taagagttct attttaggta aaattcaaac gatagaatat gcaagtttaa ttggctgtat
      841 taagcgaagt cagacacttg ctggcgcagt caatactatt tcgggtgtct tcactctatt
      901 taaaaaaagt gcagttgtcg acgttggcta ctgggatact gatatgatta ccgaagatat
      961 tgcagtttct tggaaattgc atttacgtgg atatcgtatt aagtatgaac cgcttgccat
     1021 gtgttggatg ttggttccag aaacattggg aggtctttgg aagcaacgcg tgagatgggc
     1081 tcaaggggga cacgaagtat tactacgaga cttttttagc acaatgaaaa cgaaaaggtt
     1141 tcctttatat attttgatgt ttgagcaaat catctcgatt ttatgggtat atatagtgct
     1201 tctatattta ggctatttgt tcataacagc aaacttctta gactatacat ttatgacata
     1261 tagtttttca atatttctac tatcatcatt tactatgact tttataaacg ttattcaatt
     1321 tacagtcgca ctctttattg atagtcgcta cgagaaaaag aatatggctg gactcatatt
     1381 tgtaagttgg tatccgacag tatactggat tattaacgca gcagtagttc ttgtcgcatt
     1441 tccaaaagca ttaaaacgta agaaaggtgg ttacgcaaca tggtcaagcc cagacagagg
     1501 gaatacccaa cgctaaaatc atcgctaaat attgtaagag aaacagcact tatcgctata
     1561 tcttgtgtct tttggatata ttgtttagtt gttctactcg tttatattgg tactatattt
     1621 gaaattcatg acgaaagtat caatacaata cgtgttgctt taaacattga aaatactgaa
     1681 attttagata tatttgaaac tatgggcatt ttcgcgatta tcatttttgt attttttaca
     1741 attagcatat tgattcaaaa atggcagaga ggaagagaat cgtgaagtat agaaaattta
     1801 taattttagt gttgagtatc ttgatcatat tgcctgtaag cacactggat ggtcatcata
     1861 ttgcaaatgc agatgacgat tcacctaaaa aactgaaata taaagaaaat agtgctctgg
     1921 cattaaatta tcaccgtgta agaaaagcga attttctgaa taattttatt tacttctttt
     1981 ctagtagtaa agaaattaaa aattatagtg ttagtcaatc acaatttgaa tctcaaataa
     2041 aatggctaaa atcacatgat gctaaatttt taaccttgaa agaattttta tattacaaga
     2101 aaaaaggtaa gtttccaaaa cgaagtgtat ggattaactt tgatgatatg gatgaaacta
     2161 tttatgaaaa tgcttatcca atcttaaaaa aatataaaat accggcaact gggtttatta
     2221 tcacaggtca tgttggggaa gaaaactttc acaacctcga tatgattagt aaaaaagaac
     2281 taaaagaaat gtataaaact gggttatggg aatttgaaac acatacccac gatttgcata
     2341 acttatctaa aaataataag tcaaaattaa tgaaagcttc tgaagctaca atcataaaag
     2401 atttaaacaa aagtgaaaaa tatctaacta aaaactttaa aaagtcgcag aaaactatag
     2461 cctatcctta tggcttgatg aatgacgata aattaccggt aatcaaaaaa gctgggttaa
     2521 aatacggttt ttcattagag gaaaaagcag tcactccgaa ctccaatgat tattacatcc
     2581 ctagaatatt aattagtgat gatgcttttg agcatttaat taagagatgg gacggattcc
     2641 atgaaaaaga ttagacttga actcgtatat ttacgtgcta ttatatgtgc aattattatt
     2701 atcacacatt tacttacaca aattacttta aaacatgaaa atatggaggg tggatcctta
     2761 gtgttacaat tttacattcg taatattgtg atttttggta caccttgctt tattatcttg
     2821 tcacagttac tgacaacctt gaattaccaa aaagtcacct atagatactt aactacacgc
     2881 gtaaaatata tacttattcc ttacatatta atgggattgt tttacagtta tagtgaatca
     2941 ttattaacag attcaagttt caataaacaa ttcattgaaa atgtcctatt aggtcaatgg
     3001 tatggctatt ttatcgttgt tatcatgcaa ttctttattt tgagttatat
//
LOCUS       DAKTIP010000241         3041 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.246, whole genome shotgun sequence.
ACCESSION   DAKTIP010000241 DAKTIP010000000
VERSION     DAKTIP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.246"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..803
                     /locus_tag="O0305_002557"
     CDS             <1..803
                     /locus_tag="O0305_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCY0275659.1"
                     /translation="EILKKTEYLTSFIQSYGEISLERAYYLVENYIDENCDDKVAEIY
                     KFEFQRKYDNGEFDFNHGYNFELNMLIDHYKRDVKDYDNARKYSNIYLYFGLRDFLKK
                     LMSNYSYYDSKGNIDLNEIQNDLNRFINSSASGMYERLIYNENLSNNIMFELFKKDTQ
                     DYSDLEEQLIEKFINYVVSNVKKESRSNTLIELSKILENGYTIDKEEFKKEDDYLSKY
                     IFTDIDYLKKLESKINVAIDIRSGEIHLVLDDDSLDILIQNQKYGNEF"
     gene            846..1329
                     /locus_tag="O0305_002558"
                     /pseudo
     CDS             846..1329
                     /locus_tag="O0305_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837446.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1490..1594
                     /locus_tag="O0305_002559"
     CDS             1490..1594
                     /locus_tag="O0305_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275660.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            complement(2094..>3041)
                     /locus_tag="O0305_002560"
     CDS             complement(2094..>3041)
                     /locus_tag="O0305_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCY0275661.1"
                     /translation="AIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVM
                     KRAMSHEKVKFTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMI
                     MNSVQSSYADSTHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDK
                     VKTHLANGFKAMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAEN
                     IIRVYTVMTIILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKV
                     ENDKVTTSVETKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
BASE COUNT         1113 a          374 c          514 g         1040 t
ORIGIN      
        1 aagaaatttt aaagaaaaca gagtacttaa cctcttttat acaaagttat ggtgaaattt
       61 ctcttgagcg tgcttattat ttggttgaaa actatataga tgaaaattgc gatgataaag
      121 ttgcagaaat atacaagttt gaatttcaaa gaaaatatga caatggcgag tttgatttta
      181 atcatggata taatttcgaa ttgaatatgt tgatagatca ctataaaaga gatgtaaaag
      241 actacgataa tgccagaaag tattcaaata tttatcttta ctttggtttg agagatttct
      301 taaaaaaatt aatgagtaat tattcatatt acgatagtaa agggaatata gatttgaacg
      361 aaatacaaaa cgatctgaat agatttatca actctagcgc ttctggtatg tacgagcgat
      421 taatatataa tgaaaattta tccaataata ttatgtttga attatttaaa aaggacacac
      481 aagattatag tgatttggaa gaacaattga ttgaaaagtt cataaactat gtagtgtcta
      541 atgtaaaaaa agaaagtagg agtaatactc ttatagagtt atcaaaaatt ttagagaacg
      601 gatatacaat tgataaagaa gaatttaaaa aagaagatga ttatctttct aagtacatat
      661 ttactgacat agattatttg aaaaagttag aatcaaaaat aaacgttgct attgatattc
      721 gaagtggaga aattcatttg gtattagatg atgatagcct tgatatatta atacaaaatc
      781 aaaaatacgg caatgagttt tgagtcataa ctaaatataa tatgttgaag agaggtttta
      841 tttaaatggc taaaattggt tatgatcgtg tatcaacgaa agattaatat cgatacactt
      901 aaagaatttg ggtgtgggtg tgtatatagt gaaagaataa gtggtgtcaa acgtacagag
      961 cttgttaagt gtttagatta tttacgagag agcgacacat tagttgtcta tcaacttgat
     1021 cggttaggta gaacgacaaa actattaatt gaattatcac aatgattcga tgataacgga
     1081 attgacttac aaattagtaa catgaacatt tcaacgaaag acacaatggg caaaatgttt
     1141 tttacgatga tgagtgcatt tttcaggatt agaagttaat ttactatgtg agtgtataaa
     1201 atagacttag cagcaacaag agcgagaggc cgaaaaagcg ggcgcccctc tttaccagag
     1261 aataaaaaat gagaaattaa atttttatat gatgaacaaa cgataacagg ggaagaaata
     1321 gctagttaga caagggtatg tcactcaact gtttatcgag ttgattaaga aatgaaaaaa
     1381 cttatactat gaattactgt ttaaaagtgt gcatgttata atatttattg agcaagttgg
     1441 atagatggtg gctaatctct taataaaggg gtgatgccta tggttatagt tgttactcct
     1501 agaaaggact agcatgtctg attttgaaat gcttatggtt gtattaacaa tcattggttt
     1561 agtattgatt agtactcaag accataaaaa ataaccttct attcgctttg accggcattt
     1621 ttgaaggcta tttttaaata aaatataagg tcaccgtctt tttaacgggc tcattagggt
     1681 aacatgtttc cgagtgttgc cctttttgtg tttcaagagt taatgatttt catcttttgc
     1741 ttctacttac tacaaatata ttttaacaca tttttcttat gaattgtagt tctgaacata
     1801 atcagaatta ataaaaccaa ctttccatac agcagaaaat acaattaaaa gtatagaatg
     1861 taatcacgaa ttatataaaa agtattggag acctcttcat atatagataa ttcacttagt
     1921 tattttagaa agaagcccct aacaactaaa gttgaaaaat agaggaacac agttggatgc
     1981 tacgcatcaa ctgcataaga gcccctaaca actaaagttt aaaagtagga gcgcctgcgc
     2041 taagcgcatg cataagagcc cctaacaact aaagttgtaa ggggctctaa aatttatttt
     2101 ggttgattgt cttctggttt atctgaagtc attgtttttg ttgtattatc atttaatgaa
     2161 tcttgtgctt tttctgtttt tgtttcaaca gatgttgtca ctttatcatt ttctactttt
     2221 gtattttttg gttcagtgac aactttttta ttcttgtcag ctgtttttac tttatcttta
     2281 gtaatttttt gaccacgttt taagtaatat tgaacgatac ccattaaaat gattgcatga
     2341 ataacaacga ataaaataat tgtcattact gtataaactc ttatgatatt ttctgcaaca
     2401 ctttgagaga gtgattgcgt attaaatgaa atcaagtaac caacaggtgt tttaaataaa
     2461 ataataatta agtttaataa tagtattcca atgaaaaatt tgaaaaatgt tttttgacca
     2521 tttttcattg ctttaaatcc attagctaag tgtgttttta ctttatcgtt agttgattca
     2581 acgaaactag tcataaagtt gaaaataggt attaacaata accaagtaat aattgcttta
     2641 atcaataaaa caacaatgat tatgatgctt tgagttgtaa tagcaatacc aatcaggtgt
     2701 gtactatctg catatgatga ttgtactgaa ttcataatca tttctgatac tggagaaaat
     2761 aatttgtata gtaatgatgt cagaagtgaa agaacgataa tcattgctat agatacaaga
     2821 ccgatcagaa cgctctttaa ataattacct tttttaaatg caataaataa atcggtaaat
     2881 tttacttttt catgactcat tgctcgtttc ataacatttg taattccgat aaaaatttga
     2941 actaaaacaa acagtgaaac tacagctgct ataagtaata cagcgataac ttttaaatat
     3001 gcatctactg gtggtttttg cccaaattgt gaataaattg c
//
LOCUS       DAKTIP010000242         3019 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.247, whole genome shotgun sequence.
ACCESSION   DAKTIP010000242 DAKTIP010000000
VERSION     DAKTIP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.247"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(859..2058)
                     /locus_tag="O0305_002561"
     CDS             complement(859..2058)
                     /locus_tag="O0305_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0275662.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGNLTTKIGSGKTPKGGSE
                     NYTNKGIPFLRSQNIRNGKLNLNDLVYISKDIDDEMKNSRTYYGDVLLNITGASIGRT
                     AINSIVETHANLNQHVCIIRLKKEYYYNFFGQYLLSRKGKRKIFLAQSGGSREGLNFK
                     EIANLKIFTPTIFEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFTQELRF
                     KDENGEEYPEWENKFIKDIFIFENNRRKPITSSLREKGLYPYYGATGIIDYVKDYLFN
                     NEERLLIGEDGAKWGQFETSSFIANGQYWVNNHAHVVKSNDHNLFFMNYYLNFKELRA
                     FVTGNAPAKLTHANLCNINLKIPCLTEQDKVSALLKSIDNKMNNQMNRIELLKERKKG
                     LLQKMFI"
     gene            complement(2051..>3019)
                     /locus_tag="O0305_002562"
     CDS             complement(2051..>3019)
                     /locus_tag="O0305_002562"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCY0275663.1"
                     /translation="GKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLR
                     VGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRNDDTLENPAFLGNTFDAV
                     IANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPH
                     GVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLF
                     IDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYSYSATLQEIADNDYNLNIPRY
                     VDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE"
BASE COUNT          866 a          531 c          416 g         1206 t
ORIGIN      
        1 tcgtttcaag ttttaagtct ccttttaaaa tgaatatttc acttcttata actaatattt
       61 aaacaaaatg atcacataat aatatttaat ttacaatatg ccactacagt gtcaacattt
      121 tataaacact attcatttga acatggtcta aaacgaatag gacattgtaa atgattaaat
      181 agtctgtctc tagttgatgt atatggtcat ttgttatgca gataataatt aatctaatta
      241 atttttacgt tttagttact tggttgtgat tgaggctttt tgtatctagt tgtaatattc
      301 tcctttttta gaatcataat aggtacttct tgaaattttt agaactttgc acattgctga
      361 tactgaatat tgatacgcat tttgttgaaa gacttcattt tttgtctcaa aattagcact
      421 gcttgcttta aatcatcatt ttacattatc aatcgatgtt tttctttgcg taattttatt
      481 aactcttttt catcacttgt taaaatgtct tgatgattga atgactcagt attttggttt
      541 tgttttatct aatttgagaa agttaatgat gttagatcat attctcgtat aatttcgttt
      601 ctaagcttac cattttcata aagtttaatt attttaattt aaattattta ctaaaagctc
      661 ttcagtctct tgtcataata aaatcgccta ccctcttagt ttaacaatgt ctattctcat
      721 agaatttgtc caactaaatg tagacgattc actaaacttc taaaaaaata aaccccaaat
      781 tataatagtc tcaatattaa tatattacaa tttattcata ggatttttat catttcaatt
      841 aataggttgt tatcagggtt aaataaacat tttttgtaat agtccttttt tacgttcttt
      901 taataactca attctattca tttgattatt cattttattg tctatagatt ttaacaatgc
      961 acttacttta tcttgttcag tgagacaagg tattttaaga tttatattgc ataagttcgc
     1021 atgagttaat ttagctggtg cattacctgt gacaaatgct cgtagttctt taaaatttaa
     1081 ataataattc ataaaaaaca aattatgatc attactttta actacatgcg catgattatt
     1141 tacccagtat tgcccattag caataaagct actcgtctca aactgccccc attttgcacc
     1201 atcttctcct attagtaatc gttcttcatt attgaataaa taatctttta cgtaatcaat
     1261 aattccagtt gcaccatagt aagggtataa ccccttttct cttaatgaag aagtaattgg
     1321 ttttcttcta ttattttcaa agataaagat gtcttttatg aatttgtttt cccactctgg
     1381 atattcttca ccattctcat ctttgaatcg caattcttgt gtgaagattt tctgcatata
     1441 gccttttttc tgttgttgaa gcaattcaag tttttgttct tctaattcaa tttgtcggtc
     1501 gagtttgctg aagaacttgc ctattttttg ctgttcttca aatatagttg gggtgaagat
     1561 ttttaaatta gcaatttctt tgaagtttag accttctcga ctacctccac tttgtgcaag
     1621 gaaaattttc cttttacctt ttcttgataa tagatactgt ccaaaaaaat tataataata
     1681 ctcttttttc aatctaataa tacatacatg ttgatttaaa ttagcatgcg tttcaactat
     1741 cgaattaatg gctgttctac ctattgatgc tcctgtaata tttaaaagaa catcaccata
     1801 gtacgttcta ctatttttca tctcatcatc tatatcttta ctaatataaa ctaagtcatt
     1861 aagatttaat ttaccatttc taatattttg actccttaaa aatggtatgc ctttgtttgt
     1921 atagttttca cttccacctt tgggagtctt tccactacct attttggtag taagattccc
     1981 taacttcttc tcttcccatt cgccttcaaa tcctgggaat ctcaattctg gcacattttt
     2041 cttttgtgta ttactcatct ttcaacaccc caagttcttt caggtatgca ttgatttctt
     2101 gctcaatttc tgcgatttct ttatcgatat ttttcaaatc ttgttggact tgatctaaat
     2161 caatcggtgc ttcttcttcg aatgtatcga catatctcgg tatatttagg ttgtaatcgt
     2221 tatcggcaat ctcttgtagt gtcgcgctgt agctatattt atcaattgtt tccttacgct
     2281 tatatgtgtc tataatacgt tcgacttggg catcgcttaa atgattttga ttttttcctt
     2341 tttcaaaatc attggatgca tcgataaata gtacgttgtc gtcttgttgg cgacattttt
     2401 taaatactaa aatacatgtt ggaatacttg tcccatagaa aatattcgct ggcaaaccaa
     2461 tcacagcttc taagtagttc ttttcttcaa ttaaataacg acgaatgaca ccttctgcag
     2521 cacctcggaa taatacacca tgtgggagta caacggccat ggtaccttca tcgtctaggt
     2581 aatgtaccat gtgttgaata aaggcaaagt ctgctttaga cttaggcgca agtttgccgt
     2641 aaccactgaa tcgttcgtca ttttcaaact ttgaatctgc agtccatttc gcactatacg
     2701 gtgggttcgc aataaccgca tcaaatgtat tgcctaaaaa ggctgggttt tccaatgtgt
     2761 catcattacg gatatcgaag ttctcataac gcacatcatg taataacata ttcatgcgtg
     2821 ctaagttgta tgtagtattg ttacgttctt gaccgaaata acgatacact tgtgtttctt
     2881 taccaacacg taacaacagt gaacctgaac cacatgttgg gtcatacacg tgacgtaatt
     2941 tatctttacc gtctgtgaca atcttcgcca gtatcttaga tacttgttgt ggtgtataga
     3001 actcgcctgc ttttttacc
//
LOCUS       DAKTIP010000243         3013 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.248, whole genome shotgun sequence.
ACCESSION   DAKTIP010000243 DAKTIP010000000
VERSION     DAKTIP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.248"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            97..411
                     /locus_tag="O0305_002563"
     CDS             97..411
                     /locus_tag="O0305_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275664.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
     gene            complement(417..554)
                     /locus_tag="O0305_002564"
     CDS             complement(417..554)
                     /locus_tag="O0305_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCY0275665.1"
                     /translation="MMVDKATTQIQHDCGIRVLVFIKLIESYIKYSLDNSILVRLRFV
                     V"
     gene            complement(986..1723)
                     /locus_tag="O0305_002565"
     CDS             complement(986..1723)
                     /locus_tag="O0305_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275666.1"
                     /translation="MYKVKYSSFNYYPDILLISNIAVGVIFQIEGNNGYYANEFNLMQ
                     RKNKLFSFDEELDKDFTKMFLKSIRENFLNFKGEIKEFTRFYVNNFKFTNIQIRNFDS
                     LDEAKAFIEDTTKYILHPTQEAGNKMTEAEKREYINNYLLKTFSTVQKSYVFKGAKKR
                     DKITVDFMVEDHTGKKIGYKVINNSPQALFNIRSYVAHAWFNKENLTFIMDDDMESEQ
                     RYVNSLTREFNNEAEINAILKKDLVTQ"
     gene            complement(1730..2524)
                     /locus_tag="O0305_002566"
     CDS             complement(1730..2524)
                     /locus_tag="O0305_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol kinase"
                     /protein_id="HCY0275667.1"
                     /translation="MVKILTEITSRVGNGVTTPYYAMIDSLAVVVKSINNNEGFYALF
                     NEAVGYCIAERLDFSHPDFGFAQYKSDLTINRIPNDSSFNDKEIFTYTVLENSVIHIE
                     GPGMINTIDNKDIIELIIFDSFISNTDRNKGNILIKMPKKGQKAKLFPLDYTHIFPGE
                     CIWFDVLKRGNPSIEKMVEDVFQTGNYQLLLENKSFDPIEIRKISYEIKKKLVNIDMD
                     DIISSMPNEISLGHSKQDIYLLKKFIERNISEFDDIIEEITKHLVR"
BASE COUNT         1006 a          491 c          375 g         1141 t
ORIGIN      
        1 gataacaacc agtttctcat aaatttattc taattatctt ttaaataact ttagacaagt
       61 acttgtttat atccaaatat agaaagaagg attaacatgt attttgtttt agcaatattt
      121 acaataatca gtgccagtgt aagtttaggt tattcaattc aagcatgtgc atctagccat
      181 aatataaatg catattatgc acttagtcga agcttacctt tatttttatt agctattttt
      241 tctttagtca ttcatagtgc tatatttttg ataactatat ccattgcaat gatcttagtt
      301 caatttttag atgcgattgt tggttataaa agtgaagatg tctttaaaac ttatggtcca
      361 ttagcaacat ctgtagtgaa cttaatatta ttaatagttt tcttatttta actcacttat
      421 acaacgaatc ttaatcgaac taatatcgaa ttatctaaag aatatttgat gtagctttca
      481 attaatttaa taaagaccag cactctaatg ccacaatcat gttgtatttg tgttgtcgct
      541 ttatccacca tcaatgatta ttttttacac caatcaaaaa atcgaactga tataaataag
      601 tacaaagctt atctatcaat ccgatttagt tataaaacaa aaaaagccac agtaatgtgg
      661 ctttttgtta tattcagtat caaaatggta tcaatagcca ttttcggaag tcaagaatgg
      721 cttaacaacg cggtttaaag ctatccaata ctaccttcca tttcgattga aaaaactaat
      781 ttttaaggtc ttatttttat agaaacgttg atttaatgcg atttaaaatg aagttttttc
      841 tctcgaaatt ttgaggttat tattttttgg tatcaaaaat ggtatcattt gtagttattt
      901 tagcttcgca tattaaaata accacactcc taaattaata ggtggtgtgg tttatttttt
      961 atactcgtat caatttacaa acgttttatt gtgtaactaa atcttttttt aatatagcgt
     1021 ttatctctgc ttcattgttg aattctctag ttaaactatt cacgtatctt tgctcgcttt
     1081 ccatatcatc atccataata aatgtgagat tttctttatt aaaccatgca tgagctacat
     1141 aagatctaat gttaaatagc gcttgaggag aattatttat aactttataa cctatttttt
     1201 ttcctgtatg gtcttcaacc ataaaatcaa cagttatttt atctcgtttt ttggcacctt
     1261 tgaatacata agatttttgc acagtgctaa aagtcttaag taaatagtta tttatatatt
     1321 ccctcttttc tgcttcagtc attttatttc cagcttcttg agtaggatgc aaaatgtatt
     1381 tcgtagtatc ttctataaat gctttagctt catctagact atcaaagttt ctaatttgta
     1441 tattagtaaa tttaaaatta tttacataga atctagtgaa ttcttttatt tcacctttaa
     1501 aattaagaaa gttttccctt atcgatttta aaaacatttt tgtaaaatct ttgtcaagct
     1561 cttcgtcaaa actaaaaagt ttgttttttc tttgcattag attgaattca tttgcataat
     1621 aaccattatt accttctatt tgaaaaataa cacctacagc aatattagaa atcaatagaa
     1681 tatctggata gtagttaaaa cttgaatact ttactttata cacttttcat cacctcacta
     1741 aatgtttcgt tatttcttct attatatcat caaattcact aatatttctt tcgataaact
     1801 ttttcaacaa gtaaatatct tgttttgaat gaccaagact aatttcatta ggcatagaac
     1861 tgattatatc atccatgtca atattcacta attttttctt aatttcatag ctgatttttc
     1921 gtatctctat agggtcaaaa cttttatttt ctaataacaa ttgatagttt ccagtttgaa
     1981 aaacatcttc taccattttt tctatcgatg ggttccctct ttttaaaaca tcaaaccaaa
     2041 tgcattcacc tggaaaaata tgtgtataat ccaaagggaa taacttggct ttttgtcctt
     2101 ttttaggcat tttaattaat atgttacctt tatttctgtc tgtattagat atgaatgaat
     2161 caaatattat caattcaata atatctttat tatcaattgt attaatcata cctggtcctt
     2221 ctatgtgtat aactgaattt tctaaaacag tatatgtaaa tatttccttg tcattgaaac
     2281 tcgaatcatt tggtatgcga tttatcgtta aatcagattt atactgtgca aatccaaaat
     2341 caggatgtga aaagtctaac ctctctgcaa tacaataacc tactgcttca ttgaataaag
     2401 cataaaaccc ttcgttattg ttaatcgatt taactactac agccaaagag tctatcattg
     2461 cataatacgg tgttgttaca ccattaccga ctctactcgt aatctctgtt aatattttca
     2521 ccatatctat cctccgatta attagcacaa ttacatatat aatacacaac ttacacaaaa
     2581 agaggtagac cgaacatata aacgaacaca aaaaataccc ccacgtcaga acttgtctgc
     2641 ctaaaaaggg gtgggggcgt tgtcgtttat atttaaatag taatccaacc actattaata
     2701 tgtcaatcat taaaataaaa aacaaccacc cagcaactag tatgggtggt gtagcgactg
     2761 taacaactct atgttatcaa gatatatgta tcgagtgatg acaaggaaga tgtctcctgt
     2821 gagaccaaca gccagatata tggcctctgc cgggctatat agttcactcc tactatatac
     2881 gcatgtaatt ataacataaa aaaaataggc aagtaccgaa gtacctgcct gttatgcaca
     2941 tttaaatctt gagagtaatg ttatctaaag aataatgtta ttatatcaca atacaacatt
     3001 taagcaaaca tta
//
LOCUS       DAKTIP010000244         2981 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.249, whole genome shotgun sequence.
ACCESSION   DAKTIP010000244 DAKTIP010000000
VERSION     DAKTIP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.249"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            117..1352
                     /gene="arcA"
                     /locus_tag="O0305_002567"
     CDS             117..1352
                     /gene="arcA"
                     /locus_tag="O0305_002567"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCY0275668.1"
                     /translation="MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIP
                     YLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGH
                     ETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTR
                     DPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGD
                     ELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVL
                     TMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            1438..2859
                     /gene="arcD"
                     /locus_tag="O0305_002568"
     CDS             1438..2859
                     /gene="arcD"
                     /locus_tag="O0305_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCY0275669.1"
                     /translation="MDENKLGKTSLIGLVIGSMIGGGAFNIISDMGGQAGGLAIIIGW
                     IITAIGMISLAFVFQNLTNERPDLDGGIYSYAQTGFGDFIGFSSAWGYWFAAFLGNVA
                     YATLLMSAVGNFFPIFKGGNTLPSIIIASILLWGVHFLILRGVETAAFINSIVTVAKL
                     IPIFLVIICMIVVFNFSTFKSGFYGMTSGSVGVFSWGDTMAQVKSTMLVTVWVFTGIE
                     GAVVFSGRAKSKKDVGTATVIGLISVLVIYFLMTVLAQGVIQQNQISKLANPSMAQVL
                     EHIVGHWGSVLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNKAPV
                     NALIITNILVQLFLISMLFTDSAYQFAFSLASSAILIPYTLSAFYQVKYTIQNKSKAN
                     LKQWIIGIIASIYTIWLVYAAGLDYLLLTMLLYIPGLLVYSYVQRDNNKHLTKLDYTL
                     FIFIIVLAIIGIVRLITGNISVF"
     gene            2901..>2981
                     /locus_tag="O0305_002569"
     CDS             2901..>2981
                     /locus_tag="O0305_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0275670.1"
                     /translation="MYEENIYIKNSEYEFDNNLKQLASYLN"
BASE COUNT         1039 a          405 c          558 g          979 t
ORIGIN      
        1 attgaataaa tctatatatg tgaaaaccct ccctaaacaa ttgtgaatta aatcacacga
       61 tgttttttga aagcgtttta taatgtgaat gtaataaagc aaaggaggaa tttgaaatgg
      121 tacaaggacc cattcaagta aatagtgaaa taggcaaatt gaaaactgtg ttgttaaaaa
      181 gaccaggaaa agaattagaa aatttagtac ctgatcattt aagtggttta ttattcgatg
      241 atattcccta cttaaaagtt gcacaagaag agcatgacaa atttgctcaa actttgagag
      301 atgaaggaat cgaagtagtt tatttagaaa aacttgcagc agaatctatt actgagccag
      361 aagtacgcga gaacttcata aacgacatat taacagaatc taaaaagaca atattaggtc
      421 atgaaactga aattaaagaa ttcttttcaa agttatctga ccaagaactt gtaaataaaa
      481 tcatggctgg cgtacgtaaa gaagaaattc aacttgaaac aacccattta gtagaatata
      541 tggatgatag atatccattt tacttagatc caatgcccaa cctttatttt acaagagatc
      601 cccaagcttc aattggtaga ggaatgacaa ttaacagaat gtattggaga gcacgacgta
      661 gagaatctat ttttatgaca tatatactga aacatcatcc aagatttaaa gataaagatg
      721 taccagtatg gttagatcgt aactcaccat ttaatattga aggtggagat gaattagtat
      781 tatcgaaaga tgttttagct attggtatat cagaacgtac atcagctcaa gcaatagaaa
      841 agttagcacg taatattttc aaagatgcaa acacaagttt taaaaaaatc gtagctattg
      901 aaatacctaa tacacgtaca tttatgcacc tagatacagt actaactatg attgactacg
      961 ataagtttac agtacatgca gcaatattta aagaagaaaa taatatgaat atatttacca
     1021 tagaacaaaa tgatggtaag gacgatataa aaattactcg ttctagcaag ttacgtgaaa
     1081 cacttgctga agttttagaa gtagaaaaag tggactttat tccaacaggt aatggcgacg
     1141 ttattgatgg tgcacgtgaa caatggaatg atggctcaaa cacattatgt attcgaccag
     1201 gggttgtggt gacatacgat cgcaactatg tatcaaacca acttttacgc gacaaaggaa
     1261 ttaaagtgat tgaaattact ggtagtgaac ttgtacgtgg acgcggaggc ccaagatgta
     1321 tgagtcagcc gttatttaga gaagatattt aaatttgaaa ggtggtaaaa gtcacaagta
     1381 attcattcat taaatcaaat aacaagattt ttaccacctt tattagggga gattaatatg
     1441 gatgaaaata aattaggtaa aacttcctta attggtttag tcataggctc tatgataggc
     1501 ggtggtgcat tcaatatcat ctcagatatg ggtggccaag ctggtggact tgcaataatt
     1561 atcggttgga taataactgc tattggtatg atttctcttg ctttcgtatt tcaaaattta
     1621 acaaatgagc gaccagatct tgatggagga atttatagtt atgctcaaac agggtttgga
     1681 gattttattg gtttttcaag tgcttgggga tattggtttg cagcatttct aggtaatgtg
     1741 gcttatgcaa ccctattaat gtcagctgtg ggtaactttt tccctatatt taaaggaggt
     1801 aacacacttc caagtattat catagcatca attttattat ggggtgtaca ttttttaata
     1861 cttagaggtg tagaaactgc agcgtttata aatagtattg ttacagtagc taaattaata
     1921 cctatatttc tagttattat atgcatgata gttgtattca acttcagtac ttttaaatcc
     1981 ggtttttatg gtatgactag tggaagtgtt ggcgttttta gttggggaga tacaatggca
     2041 caagtaaaaa gtactatgtt agtaactgta tgggtattca cagggattga aggagccgtt
     2101 gtcttttctg gacgtgcaaa gtctaaaaag gatgtaggaa ctgctaccgt tattggtttg
     2161 atttctgtgc tagtcattta tttcttaatg actgtactag cccaaggtgt cattcagcag
     2221 aaccaaattt caaaacttgc taatccatca atggcacaag tattagaaca tattgtaggt
     2281 cattggggtt cagtgttagt taatataggc ttaattatct ctgttttagg agcttggtta
     2341 ggatggacat tactagctgg tgaattacca ttcattgtag ctaaagatgg acttttcccg
     2401 aaatggtttg ctaaagaaaa taagaataaa gctccggtca acgctttaat tattactaat
     2461 atattagttc agttattttt aattagtatg ttgtttacag atagtgccta tcagtttgcg
     2521 ttttcacttg catcaagtgc aatcttaatt ccatatacac tcagtgcttt ttaccaggtt
     2581 aaatatacta ttcaaaataa atctaaagct aatttaaaac aatggataat aggaattatt
     2641 gcatctattt acacaatttg gttggtttat gcagctggat tagattattt actattaacg
     2701 atgttgttat atatacctgg attactcgta tacagctacg tacaaaggga taataacaaa
     2761 catttgacaa aattggatta tacgttattc atattcatca ttgtacttgc aataatagga
     2821 atagttcgtt tgattacagg taatatttct gtattttaag ttatgattta aactaagtaa
     2881 tttttaaggg gtttatagtt atgtatgaag aaaatattta tattaaaaat tcagaatatg
     2941 aatttgataa taatcttaaa caattagcat catacttaaa t
//
LOCUS       DAKTIP010000245         2976 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.250, whole genome shotgun sequence.
ACCESSION   DAKTIP010000245 DAKTIP010000000
VERSION     DAKTIP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.250"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            34..957
                     /gene="graX"
                     /locus_tag="O0305_002570"
                     /gene_synonym="apsX"
     CDS             34..957
                     /gene="graX"
                     /locus_tag="O0305_002570"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCY0275671.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            973..1647
                     /gene="graR"
                     /locus_tag="O0305_002571"
                     /gene_synonym="apsR"
     CDS             973..1647
                     /gene="graR"
                     /locus_tag="O0305_002571"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCY0275672.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            1640..2680
                     /gene="graS"
                     /locus_tag="O0305_002572"
                     /gene_synonym="apsS"
     CDS             1640..2680
                     /gene="graS"
                     /locus_tag="O0305_002572"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCY0275673.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            2824..>2976
                     /locus_tag="O0305_002573"
     CDS             2824..>2976
                     /locus_tag="O0305_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCY0275674.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLN"
BASE COUNT         1113 a          363 c          559 g          941 t
ORIGIN      
        1 gttttttggt tacagtttaa aggagtattt tagatgaaac ctaaagtttt attagcaggt
       61 ggaacaggat atattggtaa gtatttaagt gaagtgattg aaaatgatgc tgaacttttt
      121 gctatatcaa aatatccaga caataaaaaa acagatgatg ttgaaatgac ttggattcag
      181 tgtgatatat ttcattacga acaggttgtt gcagcaatga atcaaataga tattgctgta
      241 ttctttatcg acccaacaaa gaattctgcc aaaataacac aatcatcagc aagagattta
      301 acattaatcg cagcagataa ttttggtcga gcagcggcta tcaatcaagt aaaaaaagta
      361 atctacatac ctgggagtcg ttatgataat gaaacaattg aacgcctagg tgcatatggt
      421 acacctgtag aaacaacaaa tttagttttt aaacgttctt tagttaatgt agaattacaa
      481 gtttcaaagt atgatgatgt tagatcaacg atgaaggtag ttttaccaaa gggatggaca
      541 ttaaagaacg ttgtaaacca ttttattgca tggatgggtt acactaaagg aacttttgtg
      601 aaaacagaaa aatcacatga tcaatttaag atatatatta agaataaggt gcgaccgctc
      661 gcagtattta aaatagaaga aacagctgat ggaataataa ctttaatttt attgagtgga
      721 agtttagtga aaaaatatac agttaatcaa gggaagttag aatttagatt aatcaaagag
      781 tcggcagtcg tttatataca tctatacgat tatatccctc gattattttg gccgatttat
      841 tactttatac aagcaccaat gcaaaaaatg atgattcatg gctttgaagt tgactgccgg
      901 attaaagatt ttcaaagtcg attaaaatca ggagaaaata tgaaatatac taaatgatat
      961 tgggtgatat ggatgcaaat actactagta gaagatgaca atactttgtt tcaagaattg
     1021 aaaaaagaat tagaacaatg ggattttaat gttgctggta ttgaagattt cggcaaagta
     1081 atggatacat ttgaaagttt taatcctgaa attgttatat tggatgttca attacctaaa
     1141 tatgatgggt tttattggtg cagaaaaatg agagaagttt ccaacgtacc aatattattt
     1201 ttatcatctc gtgataatcc aatggatcaa gtgatgagta tggaacttgg cgcagatgat
     1261 tatatgcaaa aaccgttcta taccaatgta ttaattgcta aattacaagc gatttatcgt
     1321 cgtgtctatg agtttacagc tgaagaaaaa cgtacattga cttggcaaga tgctgtcgtt
     1381 gatctatcaa aagatagtat acaaaaaggt gatcagacga ttttcctgtc caaaacagaa
     1441 atgattatat tagaaattct tattaccaaa aaaaatcaaa tcgtttcgag agatacaatt
     1501 atcactgcat tatgggatga tgaagcattt gttagtgata atacgttaac agtaaatgtg
     1561 aatcgtttac gaaaaaaatt atctgaaatt agtatggata gtgcaatcga aacaaaagta
     1621 ggaaaaggat atatggctca tgaataattt gaaatgggta gcttattttt tgaaatctcg
     1681 catgaactgg atattttgga tattgttttt aaacttcctt atgttaggca ttagtctaat
     1741 cgattatgat tttccaatag acagtttatt ttatattgtt tctttgaatt taagtttaac
     1801 aatgattttt cttttattga catattttaa agaagtaaaa ttatataagc attttgacaa
     1861 agataaagaa atagaagaaa ttaaacataa agatttagcg gaaacgccat ttcaacgtca
     1921 tacagttgat tatttatatc gtcaaatctc agcgcacaaa gaaaaggttg ttgagcaaca
     1981 gttgcaattg aacatgcatg aacaaaccat tacagaattt gtgcacgaca taaaaacacc
     2041 tgtgacagct atgaaattat taattgatca agaaaaaaat caagaaagaa aacaagcatt
     2101 actatatgaa tggtctcgta taaactcgat gctagataca cagctgtata ttactagatt
     2161 agaatctcaa cgtaaagata tgtattttga ttacgtgtca cttaaacgca tggtcattga
     2221 tgaaatacaa ttaacaagac atattagtca ggttaaaggt attggttttg atgttgactt
     2281 taaagtggat gattatgttt atacagatat aaaatggtgt cgtatgatta ttagacaaat
     2341 tttgtcaaac gcattgaaat atagtgagaa ttttaatatt gaaattggga cagaattaaa
     2401 tgatcaacat gtttcgttat atattaaaga ctatggcaga ggtattagta aaaaagatat
     2461 gccgcgaata tttgaacgag gatttacgtc aacggctaac agaaatgaaa cgacgtcttc
     2521 aggtatgggt ctatatttag taaatagtgt aaaggatcaa ttaggtattc acctgcaagt
     2581 cacgtcgact gttggtaagg ggacaactgt cagattgatt ttcccattac aaaatgaaat
     2641 tgttgaacgc atgtcggaag tgacaaattt gtcattttaa acatgcgttt tgttacttag
     2701 aattgataca tcaatgcagc ttcaacgtta taataagata gatgttagtc atatgttaaa
     2761 ttgaagatac aagtgccaaa gcctaaagga aatgaagtta agataaatta taggagtgtt
     2821 aaagtggcaa ttttagaagt aaaacaatta acaaaaatat atggaactaa aaaaatggca
     2881 caagaagtgt tgcgagatat caatatgtct attgaagaag gcgagtttat tgctattatg
     2941 ggtccctctg gatctgggaa aacgacatta ttaaat
//
LOCUS       DAKTIP010000246         2970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.251, whole genome shotgun sequence.
ACCESSION   DAKTIP010000246 DAKTIP010000000
VERSION     DAKTIP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.251"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..212
                     /locus_tag="O0305_002574"
     CDS             <1..212
                     /locus_tag="O0305_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275675.1"
                     /translation="DKEKNRETVKNYAWNLQKSINDIMQSYPSTKFVQFYKDDVSPSM
                     VDGNGRLKSDTNVISIEGGKYDENK"
     gene            199..501
                     /locus_tag="O0305_002575"
     CDS             199..501
                     /locus_tag="O0305_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275676.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            655..1014
                     /locus_tag="O0305_002576"
     CDS             655..1014
                     /locus_tag="O0305_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275677.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(1099..1710)
                     /locus_tag="O0305_002577"
     CDS             complement(1099..1710)
                     /locus_tag="O0305_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0275678.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(1789..2265)
                     /locus_tag="O0305_002578"
     CDS             complement(1789..2265)
                     /locus_tag="O0305_002578"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCY0275679.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(2268..2768)
                     /locus_tag="O0305_002579"
     CDS             complement(2268..2768)
                     /locus_tag="O0305_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275680.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
BASE COUNT          998 a          556 c          417 g          999 t
ORIGIN      
        1 atgataaaga aaaaaataga gaaacagtaa aaaactatgc ttggaacctt caaaaatcta
       61 taaatgacat tatgcaatca tatcctagca caaaatttgt acagttttat aaagatgatg
      121 tttccccgtc aatggtagat ggaaatggcc gtttaaaatc ggatactaat gtaatttcca
      181 tcgagggagg aaagtatgat gagaacaaat gatcatataa ccgatttaga tgctattgag
      241 ttaagtagcg gttggaatta caattcgaaa ttggagactg gcaaaaatgc actactatca
      301 gatagccttt taagagatat taaagtatta agttctaatg ctaaaggcga cgctgacata
      361 ctagaaacag aacttcgtgc tgtaataaac tcaatgtctc aaaacgaagg taaatctgtt
      421 gatgcactta aaaatagtac cgcttctcta aaaaacaatc taaccaaatt aaaagagcaa
      481 ctcgacaaaa ctacacatta atttcataca ttttgagtga acagaattat atttccatag
      541 caaacattcc taaactacac atacgttaca taattgattc atttttatag aaacgggtaa
      601 aaatgataaa gtaatcatct ttcacaaaaa atcaaatatt gggaggaatt aaatatgatt
      661 ttgcgttacg caacgaattt aaaacttgcg aaagagctat accaagccag taaacctaaa
      721 ttgcaagaag ataagggtat gattgaactg tttgaacata cttttggctt gcaaaaagaa
      781 cttgtaaaat acgtcggcat tgcagaagca actactgctg ctttatactc agctagcttt
      841 atcaataaaa acatttctcg ccttgcatca ctatcaacga taggcatttt aagtgtagct
      901 gcttacaaac atttcgaagc tggccaaggt aaaaaaggtg cacaacatgc attaaattta
      961 ttaggtcttg ctactttaag tttactagat acattttctt gtaataaaaa atagtcttca
     1021 ttttagaagc acatacttta gcacctgtgt acttttgatt caattcataa acggtatacc
     1081 gctatagcat gtgttttatt atttttctgg tctctttatc gtaaatattc cccctaattt
     1141 agcataatca tttccggcta aacgagaaac agcctgtaat gcgtgtggat tgattttaaa
     1201 atcaccctca taaatactgt catcaatatg ataagttaca atttcaccta ttattaaatc
     1261 agccccatct aatacatctc caagcaatat catttgcgat agtttacatt cgaatctcat
     1321 tttcgcatct ttaattcctg gcgtcttaat cgttgtagat gttaaaagtg ataattctgt
     1381 acgactcaac tcactgtcac catatgctaa cggcgctgca gtctcattaa tatcttgaac
     1441 attatcttcg tctgtaatat gcacaacaaa gtctccagtc cgttctatat ttaatgcagt
     1501 atcttttctc ttacctcctg cacgttgaac tgcaatagca atcattggcg gatgattatt
     1561 aacaatatta aaaaagctaa atggtgctgc atttactgat gcatcttgat ttaatgttgt
     1621 aacaaaagct ataggtcgtg gaataattga accaattaat aatttatagt tttctctagc
     1681 agttaatgat tgtgcatcaa acgtatacat aatacctacc tcttttctaa gtatatctag
     1741 gtatttctcc gattttggtt aatttaaaca tctattctcc tctgaaaatc acttgtattt
     1801 atttagcaaa tcttttgaaa tatgacacat atgcatatct tctggatatt tttctaaatg
     1861 ttgctgatgt tcttcagcac ttttaatgta gttagacagc ggtaagactt ccactgcaat
     1921 ttgatctctg tctttacgtc gttcaatgaa ctgacgcgct tcaattaagt ggtcatctac
     1981 acaactatat aaacccgttc gatacttttg tccaatatca tttccttgtt gattcacact
     2041 gtaaggatca atgatttcaa ataaataatt cataatgtct gtaattgtta acatacgatc
     2101 atcgaaatga agtttgacac attcagcata accatcatac ggaccgtcta atttagagct
     2161 tcttccattt gctcttcctg cttctgtatg tataattcca ggtattgttg caaaaaatgc
     2221 ttcaacaccc cataaacatc ctcctgctac ataaacaact gccatattta cacctcatca
     2281 tcctttttta tatttttaac aaggttatac catttaatac cgccatgaca tgattctgat
     2341 acaccttcat tacgataccc atatttttca taaaatgaaa ttaatgattc tcgacatgtt
     2401 aacgttacac catgtcgatg atgattctta gcaagagttt caaaatagtt tagtaagcga
     2461 cctgcaatac cctgaccttg ataatttggt gctacaacaa gacctaacac actaatatag
     2521 ccaccttcac tattatttgt ggagacattt ttaaataaat catcgctaat gtaacgctct
     2581 tttatgactg gaccgttgat gtatccaatt acttcatcat tcttttcagc tacaataaaa
     2641 gtttcttgaa tttgttcaat tctcaatttc aatgcctctc gtgttgcagc ttcttccggt
     2701 gaaaaaccaa tattttcaag tgcaataatt tgatctaagt cctgcatgat tacttttctt
     2761 aataccataa aaacacttcc ttagccctat atatcaaaag taataccttc atctttatat
     2821 tcattatttt caaactacct tcactataaa aaacgagtct ttttattgaa agccattcgc
     2881 cttaatcaat tagacaagtt gtattatacg aattagtaaa taatcatgat tataattcat
     2941 tttaagcata ctcaaaaaga ctggtacatg
//
LOCUS       DAKTIP010000247         2968 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.252, whole genome shotgun sequence.
ACCESSION   DAKTIP010000247 DAKTIP010000000
VERSION     DAKTIP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.252"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..246
                     /locus_tag="O0305_002580"
     CDS             <1..246
                     /locus_tag="O0305_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCY0275681.1"
                     /translation="NDNVIPYVDEAIKIIDESGLHFRVGPLETTVQGNMNECLILIQS
                     LNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            380..1924
                     /gene="miaB"
                     /locus_tag="O0305_002581"
     CDS             380..1924
                     /gene="miaB"
                     /locus_tag="O0305_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCY0275682.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFIKEVE
                     PEMVIQ"
     gene            1925..2290
                     /locus_tag="O0305_002582"
     CDS             1925..2290
                     /locus_tag="O0305_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCY0275683.1"
                     /translation="MYNKDDVLNQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            2317..2808
                     /gene="thiW"
                     /locus_tag="O0305_002583"
     CDS             2317..2808
                     /gene="thiW"
                     /locus_tag="O0305_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCY0275684.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
BASE COUNT         1109 a          384 c          560 g          915 t
ORIGIN      
        1 aatgacaatg ttatacctta cgtagacgag gcgattaaaa taattgacga atctggtttg
       61 cattttagag taggtccgtt agaaacgaca gtacaaggaa atatgaatga atgtttaatt
      121 ttaatacaat cattaaatga acgaatggtg gaacttgaat gtccaagtat tattagccaa
      181 gttaagtttt atcatgtgcc agatggcatc actattgaaa ctttaactga aaaatatgat
      241 gaataacatt aaaagtgaag taaactggat ttgaattggc ttgttagaga tgacgtataa
      301 ctttaactgt ttttgcactt tatagttaaa tttaatataa ttattaaatg atacgggcaa
      361 atagaaagga ttttgtaaag tgaacgaaga acaaagaaaa gcaagttctg tagatgtttt
      421 agctgagaga gataagaaag cagaaaaaga ttatagtaaa tattttgaac atgtttatca
      481 gccgcctaat ttaaaagaag cgaaaaaaag aggtaaacaa gaagttcgtt ataatagaga
      541 tttccaaatt gatgaaaaat atcgcggtat ggggaacgag cgtacatttt taattaaaac
      601 atatggatgt caaatgaatg cacatgacac tgaggtcatt gctggtatac ttgaagcatt
      661 aggctatcaa gcaacgactg atattaacac tgcagatgtt attttaatta atacatgtgc
      721 gattagagaa aatgccgaga acaaagtgtt tagtgaaata ggtaatttga agcatttgaa
      781 aaaagaacga cctgatattt taatcggtgt ttgtggttgt atgtcacaag aagagtcagt
      841 agtgaataaa attttaaaat cgtatcaaaa tgtagatatg atatttggta cacataatat
      901 tcatcattta ccagaaattt tagaagaagc atacttatct aaagcaatgg ttgttgaagt
      961 atggtctaaa gaaggagacg ttattgaaaa tcttccaaaa gtccgtgaag gcaacattaa
     1021 agcatgggtc aatattatgt atggttgtga taagttttgt acatattgta ttgttccatt
     1081 tacaagaggt aaagaacgaa gccgtagacc tgaagacatt atagatgaag tacgtgaact
     1141 tgctcgtgaa ggttacaaag aaataacgct tttaggtcaa aatgtaaatt cttatggtaa
     1201 agatttacag gatatagaat atgacttagg agatctttta caagcaattt ctaaaatagc
     1261 gattccaaga gttcgtttca caacaagtca tccttgggac tttacagatc acatgattga
     1321 tgttatttca gagggtggta atatcgttcc gcatatccac ttgccagttc aatctggaaa
     1381 taatgcagta ttaaaaataa tgggtagaaa atatacacga gaaagttatt tggatttagt
     1441 aaaacgaatc aaagatagaa ttcctaatgt agcattaact acagatatta ttgtagggta
     1501 tccaaatgaa tcagaggaac aatttgaaga aactttaact ctgtatgatg aagttggttt
     1561 tgaacatgca tatacgtact tgtattcaca acgtgatggt acgcctgctg ctaaaatgaa
     1621 agataatgta cctttaaatg tcaaaaagga acgattgcaa cgtttgaata aaaaagttgg
     1681 tcattattca caaatagcta tgagtaagta cgaaggacaa actgtaacag tactttgtga
     1741 aggaagtagt aaaaaagatg atcaggttct tgctggctac actgataaaa ataagctagt
     1801 taatttcaaa gcgcctaaag aaatgattgg taaactagtg gaagtacgaa tagatgaagc
     1861 taaacagtat tcattaaatg gcagttttat aaaggaagta gagccggaaa tggtgattca
     1921 ataaatgtat aataaagatg acgtgttgaa ccaagcggat aatattgcaa ataaaattaa
     1981 aaatttggat actatcaaaa catatcaaca aattgaagca cagattcatc agaaccaaac
     2041 gataaagact aaaatggata tgttaaaaaa gcatcaaaaa caagcagtaa actttcaaaa
     2101 ttacgggaaa caaaatgcgc tagaacagtc ggaacatacc attcagagta tagaagcaga
     2161 aataaataca ttgcccatag ttgaacagtt tcaaacttca caatatgaag cgaatcaatt
     2221 attgaaaatg tttgtatcaa caatggaaac acgtttaaat gaccataata aagccaagca
     2281 tagtgattaa ttacaaacaa aaaggagaac aatcatatga aatcaagaaa actggctata
     2341 actgcacttt taattgcaat aaatgttgta ttaagcagta ttatcatcat tcctctagga
     2401 ccagttaagg cagcaccagt acagcatttt gtaaatgtat taagtgcggt catagtaggt
     2461 ccttggtatg gattagctca agcgcttata tcatcaattt taagagttct ttttggtact
     2521 ggtacagctt ttgcatttcc gggtagtatg attggagttt tattggctag tatgttttac
     2581 atatatcgta aacatatatt tatggccgcg gtcggtgaag tacttggaac tggtgtcatc
     2641 ggaagtttaa tttgtatacc attagcatat ttccttgggc ttcaagactt cttcattaaa
     2701 ccgttaatga ttacgttcat agtctcaagt gctatcggat ctattataag ttatttctta
     2761 ttaattactc taaaaaaacg tggtattctt caaaggttta taaaataatt ttatgatata
     2821 tgatttcaaa cagagtcatt gttcatttta atattgaacg cccttactta attaaatgtc
     2881 ttgaatgtta gaattttaat ttaattctaa acatgctatt gacacatttt attaggtttg
     2941 aggcgttttc ttatacattt aagatgct
//
LOCUS       DAKTIP010000248         2936 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.253, whole genome shotgun sequence.
ACCESSION   DAKTIP010000248 DAKTIP010000000
VERSION     DAKTIP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.253"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(109..504)
                     /gene="spxA"
                     /locus_tag="O0305_002584"
     CDS             complement(109..504)
                     /gene="spxA"
                     /locus_tag="O0305_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCY0275685.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            799..1788
                     /gene="trpS"
                     /locus_tag="O0305_002585"
     CDS             799..1788
                     /gene="trpS"
                     /locus_tag="O0305_002585"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCY0275686.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(1830..2711)
                     /locus_tag="O0305_002586"
     CDS             complement(1830..2711)
                     /locus_tag="O0305_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275687.1"
                     /translation="MQNKSKSPFKIAFSKFIHNKIAMLSVIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGGIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGIKDAFNPRGRR"
     gene            complement(2723..>2936)
                     /locus_tag="O0305_002587"
     CDS             complement(2723..>2936)
                     /locus_tag="O0305_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCY0275688.1"
                     /translation="ADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLF
                     FSFLGLLGNLISDITYGFIDPRIRSN"
BASE COUNT         1072 a          480 c          439 g          945 t
ORIGIN      
        1 aaagatacta agcaattatt gtaaaattaa aaccaaaaaa gcaacactat agaaatagtg
       61 ttgcttgaca tatcttttag taaagtatga cacatagtta aatggttatt agtcaaccat
      121 acgttgtgct tcttgtaatt ggaacgtacg aacttttcta ggtaagaaac gtcgaatctc
      181 gtcctcatta taaccaactt gtagtcgttt attatctaaa ataattggac gacgtaataa
      241 gccaggatta tcttgaatga ttgaatataa gtcttgtaat ggtagtgaat caatatcaac
      301 atttaatttt tggtatgttt tagaacgtgt agaaatgatt tcatcagtac cgtcttcagt
      361 catttttaat atttgcttaa tttcatcaat tgttaaatgt tcagaaaaaa tattacgctc
      421 cgtatacgga atgtcatgtt cttgtaacca tgctttcgct ttacggcaag atgtgcaact
      481 tggtgaagta aataatgtta ccatacatct cactctccta tttgaatgaa taaaattcat
      541 tgcttaaaat ttagttatag atcaagaaaa aacatttttt tctaaaattc ttaatcgtta
      601 ctatttatta taactatcta acattaaaat taaatgagaa aaacctaatt tttcagataa
      661 gtttctacac ttataaaaaa gattattaat ccctttgtta gtagtaagtt atacgtatat
      721 tctaacacat cttacatttt taagaaatac tgttataatg ataattatta aaatattact
      781 aagaaagtag gcatttaaat ggagacatta ttttcaggca tccaacctag tggaattcct
      841 actattggaa attatattgg cgcactaaaa caatttgttg atgtgcaaaa tgactatgat
      901 tgttatttct gtatcgtaga tcaacatgca attacaatgc cacaagatcg tttaaaatta
      961 cgtaaacaga ccagacaatt agcagcgatt tatttagctt ctggtataga tccagacaaa
     1021 gcaacattgt tcatacaatc tgaagtccct gcacacgtac aagcaggatg gatgttaact
     1081 acgattgctt ctgttggaga attagagcgt atgacgcaat acaaagataa agctcagaaa
     1141 gcagttgaag gtatacctgc tggtctatta acatatccac ctttaatggc agctgatatt
     1201 gttctttaca atactaatat cgttccagtt ggagatgacc aaaagcagca tatcgaattg
     1261 actcgtaacc ttgtagatag atttaatagt cgctataatg atgtgcttgt gaaacctgaa
     1321 attcgtatgc ctaaagttgg tggacgtgtc atgagtttac aagacccaac aagaaaaatg
     1381 agtaagagtg atgataatgc taaaaacttc atttcattat tagacgagcc gaatgttgca
     1441 gctaaaaaaa ttaaaagcgc agtaactgat tcagatggta ttattaaatt tgatcgtgac
     1501 aacaagccag gtataacaaa tttaatttca atatacgctg gattaacaga catgccaatt
     1561 aaagatattg aggcaaaata tgagggcgaa ggttatggta aatttaaagg tgaccttgct
     1621 gaaatagtta aagcattttt agtagaattc caagaaaaat acgaaagttt ctataactca
     1681 gataaacttg atgatatttt agatcaaggt agagataaag cacacaaagt ttcatttaaa
     1741 actgtcaaaa aaatggaaaa agcgatgggt ttaggacgta agagataaaa taaagaggta
     1801 ctagaattaa ctagtgcctc ttttattatt taacgtctac ctctaggatt aaaagcatct
     1861 ttaatccctt cccctacaaa gtttatactt aatatagtta atgtaatcat tatagcaggt
     1921 ggcatccata tccacggttt accacttata acatcccctt cttgagcatc actcaacata
     1981 ttaccccaag atggtattgc tttactaatt ccgaatccta aaaagctcaa tcctgattcc
     2041 actacaatca taccggcaaa taacaatgtt gcttgtacga taactacact taatatattc
     2101 ggcaaaagat gtttcaaaat aattttatat gtgggtgtac caattgattt tgctgccaaa
     2161 aagtattcat tttctttttc ttgaagtact ttaccacgaa caagtcttgc aatacctccc
     2221 caacttaata cgataataac tagaacaaga attatggcag atccataagg atttttaatt
     2281 ttatctccaa gtgcagcatt taatacaatt gcaaatatta aaaatggaaa taacataaca
     2341 aattcggtta ttctcattaa taatgtatca acaatacctc caaaataacc agaaatcact
     2401 ccaactgtaa ttccaatcaa aagcattcct attgtagatg taattccaat ggataatgaa
     2461 atacgacctg catataacaa acgactaaag ttatccctac caccagagtc tgtaccaaga
     2521 atgttttgtg ctgtcatttc accttttata tttaataaat cttgttggtt cactggaaaa
     2581 ggagctatta atggcgctat aattgataca atagttatga ttaataaaaa aataaccgat
     2641 aacattgcaa ttttattatg aataaattta gaaaatgcaa ttttaaaagg cgattttgac
     2701 ttattttgca ttctaatccc ccctagttac ttctaattct tggatctata aatccataag
     2761 taatatcaga aatcaaatta cccagtaaac ctaaaaatga gaaaaataac gttaatgcca
     2821 tcattaatgg atagtcttgg cctattaccg attctaaaaa taatttaccg ataccgttat
     2881 atgaaaagat agtctccgta atcacggctc cacctaaaat acttacaata tcagca
//
LOCUS       DAKTIP010000249         2925 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.254, whole genome shotgun sequence.
ACCESSION   DAKTIP010000249 DAKTIP010000000
VERSION     DAKTIP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.254"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            349..1047
                     /locus_tag="O0305_002588"
     CDS             349..1047
                     /locus_tag="O0305_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCY0275689.1"
                     /translation="MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYIN
                     MLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQK
                     EEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDV
                     MDSKQIKNIEVNLN"
     gene            1413..2096
                     /locus_tag="O0305_002589"
     CDS             1413..2096
                     /locus_tag="O0305_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCY0275690.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(2181..2282)
                     /locus_tag="O0305_002590"
     CDS             complement(2181..2282)
                     /locus_tag="O0305_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275691.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            2360..>2925
                     /locus_tag="O0305_002591"
     CDS             2360..>2925
                     /locus_tag="O0305_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCY0275692.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPEDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFL"
BASE COUNT         1160 a          378 c          517 g          870 t
ORIGIN      
        1 agcggtttaa taatcccatg tttaatgatt ttgatacgtg tttttataat aaaaacatat
       61 cgaacattga ctacgttatt aagctgcttt tttgtacact ttataaccaa tagcttaaga
      121 tttaaaacta atcggaaaga acaatgattc accaaaaaaa tatttatgtt gctattaaaa
      181 atcagttaat acgaatgtta aaatacgttt gattttcatt aataatgatt caagtttatt
      241 taaatgagcg ttaatgtcag tctgttttga tgcaccttat aataaagaca gatagttcaa
      301 attacgtaat aataacaatc caatacatta agattggagc aaaaaaatat gaaattaaca
      361 acgatagcta aagcaacatt agcattagga atattaacta caggtgtgtt tacagcagaa
      421 agtcaaactg gtcacgcgaa agtagaactt gatgagacac aacgcaaata ttatatcaat
      481 atgctacatc aatactattc tgaagaaagt tttgaaccaa caaacattag tgttaaaagc
      541 gaagattact atggctctaa cgttttaaac tttaaacaac gaaataaagc ttttaaagta
      601 tttttacttg gtgacgataa aaataaatat aaagaaaaaa cacatggcct tgatgtcttt
      661 gcagtacctg aattaataga tataaaaggt ggcatatata gcgttggcgg tataacaaag
      721 aaaaatgtga gatcagtgtt tggatttgta agtaatccaa gtctacaagt taaaaaagtt
      781 gatgctaaaa atggcttttc gataaacgag ttgtttttta ttcaaaagga agaagtatca
      841 ttgaaggaac tggactttaa aataagaaaa ctcttaatcg aaaaatatag attgtataaa
      901 ggaacgtctg ataaaggtag aattgttatc aatatgaaag acgaaaagaa gcatgaaatt
      961 gatttaagtg aaaaattaag ttttgaacgt atgtttgatg taatggatag taagcaaatt
     1021 aaaaatattg aagtgaattt gaattagttc gagttaatag cataatagct taagaagcgg
     1081 cttaacgaca aaatgtgaat tggcatttgt gtccttatat aaggaactgt gttaaataca
     1141 ttactgttgt taagttgttt ttgtaattca aagagcagaa cagagtaaca tcatcagttg
     1201 tagtaaacga taatccggta aaacaactaa atgaaataat gaaagtcatt taacctgaac
     1261 attaaaatat atttgttttt cattaagaat aattcaagta tatttaaatc gaggttaatt
     1321 atcgtatgaa acgatgcacg ttataataaa aatgtatgat tcaaattacg taatgaaaac
     1381 aatccaatat attaagattg gagcaaataa atatgaaatt tacagcatta gcaaaagcga
     1441 cattagcttt aggaatttta acaacaggaa ctttaacaac agaagttcat tcaggtcatg
     1501 caaaacaaaa tcaaaagtca gtaaataaac atgacaagga agcattatac cgatactaca
     1561 ctggaaagac tatggaaatg aaaaatatta gtgctttgaa acatggtaaa aacaacttac
     1621 gttttaagtt tagaggtatt aagattcaag ttttactgcc tggaaatgat aaaagtaaat
     1681 ttcaacagcg tagttatgag gggttagatg ttttctttgt tcaagaaaaa agagataagc
     1741 acgatatatt ttatactgtt ggtggtgtaa tacagaataa taaaacatct ggagttgtca
     1801 gtgcaccaat attaaatatt tcaaaagaaa agggtgaaga tgcttttgtg aaaggttacc
     1861 cttattacat taaaaaagaa aaaataacac taaaagaact ggattataag ttgagaaagc
     1921 atctaattga aaaatacgga ctttataaaa caatctcaaa agatggtagg gtcaaaatta
     1981 gcttgaaaga tggcagtttt tataaccttg atttaagatc taaattaaaa tttaaatata
     2041 tgggggaagt catagaaagc aaacaaatta aagatattga agttaactta aagtaaatca
     2101 ttacgaataa ttaaaagtaa ttgaagcggc ttaacggtga aatgtaaatt ggtgcgcata
     2161 gcttatacaa aaaggatgca tcaatcgata tcgtcgttaa gccgttttgg tttgtgtgtc
     2221 atgaatccta tcccaatctc cataaaggta aaatttccac caccaacatc aaaattctcc
     2281 acatcgcaac ataaccaaat gttataataa atctattaca caaagagata aattacttat
     2341 tcaaaggcgg aggaatcaca tgtctattac tgaaaaacaa cgtcagcaac aagctgaatt
     2401 acataaaaaa ttatggtcga ttgcgaatga tttaagaggg aatatggatg cgagtgaatt
     2461 ccgtaattac attttaggct tgattttcta tcgcttctta tctgaaaaag cggaacaaga
     2521 atatgcagat gccttgtcag gtgaagacat cacgtatcaa gaagcatggg cagacgaaga
     2581 ataccgtgaa gacttaaaag cagaattaat tgaccaagtc ggttacttca ttgagccaga
     2641 agatttattc agtgcgatga ttcgtgaaat tgaaacgcaa gatttcgata tcgaacacct
     2701 ggcgacggca attcgtaaag ttgaaacatc aacattaggt gaagaaagtg aaaatgactt
     2761 tatcggtctg ttcagcgata tggatttgag ttcaacgcga ctaggtaaca atgtcaaaga
     2821 acgtactgct ttaatctcta aagtcatggt taatcttgac gacttaccat tcgttcacag
     2881 tgacatggaa attgatatgt taggtgatgc atatgaattc ctaat
//
LOCUS       DAKTIP010000250         2779 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.255, whole genome shotgun sequence.
ACCESSION   DAKTIP010000250 DAKTIP010000000
VERSION     DAKTIP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.255"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..750)
                     /locus_tag="O0305_002592"
     CDS             complement(<1..750)
                     /locus_tag="O0305_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCY0275693.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILKGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKS"
     gene            complement(747..1439)
                     /locus_tag="O0305_002593"
     CDS             complement(747..1439)
                     /locus_tag="O0305_002593"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCY0275694.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNRDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(1456..2118)
                     /locus_tag="O0305_002594"
     CDS             complement(1456..2118)
                     /locus_tag="O0305_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCY0275695.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT          893 a          486 c          384 g         1016 t
ORIGIN      
        1 cgacttcact tttcctggct tagtcggtct gtctaaataa acaaaattat cattgattac
       61 tgaactggca tttcgaagta atgcttcaat atcattcgaa tatttcttca atttatgatt
      121 gtgaaataat tcttgcatca aaaatggtct ttggtcacat gaatgtgcat ctgaagctac
      181 aaaatgagcc aaattacatt ctataaattg taatgataac ttttgaatgt ttttaccaaa
      241 tccaccaact aaagaactcg atgttaattg actcagtgcc ccatttgcaa ccaattcata
      301 taatatttcc ggatttttgg cgatacttct atttctttca ggatgtgcaa tgattggtat
      361 gtaacctctc gattgtattt caaaaaacaa ttgttttgta taatgtggta cttcgcccgt
      421 tggaaattca attaataaat atttcgaacg attaatacct tgaatactac cattatctaa
      481 gcctttcaga atcgaatctg taattctaat ttcttgcccg ggaaataatt taatatccaa
      541 tgcttgaact tctggatgcg ttcttaactc cgccaatttc acaagcactt gttgaaatgt
      601 attatcatat ctcggatgca aatgatgagg tgtcgctaca atacttgtta caccttcatc
      661 cttagcttgc tttaatagtg caatactctt ttcaattgtt ttaggaccat catctatatc
      721 aactaatata tggttatgaa tatcaatcat gattcatcag tcccataata tgcatagtaa
      781 ctagcacttt tatctttagg cattctattt aagactacac ctaataattt agcacctgtt
      841 gcttcaataa gttcttttcc ttttttaact tcatctctat tattattttc cgaattaact
      901 acgtagacaa cattgccggt aaactttgaa aataattgcg catctgtaac tgtgttcact
      961 ggtggcgtat cgataattac aaagttataa ttcatcaata atgtgtcata caaatttgca
     1021 aatgcccttg atgtaattaa ctctgacgga ttcggtggga ttggcccaga cgtcaagacg
     1081 tctaaatctt gaatttcagt tgagataata ctgtcttgat aagttgacca atttagcaat
     1141 aaacttgata ggccttcatt gtttggcaaa ttaaaaatat aatgctgcgt aggtttacgc
     1201 atatccccgt ctacgattag tgttttataa cctgcttgcg catatgcaac tgctaaattt
     1261 gctgcaattg tagacttacc tgcgcctggt gcctctgaag tgattacaat gctttgaact
     1321 gcactgtcag gatttgcaaa cataatattt gatcttatgc ctcgaaattt ctcgctaata
     1381 ggtgactttg gttgttcatg gacaattaaa cttgatgtac ttcttcgtgt attcgtcatg
     1441 gtaattcctc gtaaattaaa atttttgtat tgaacctaaa ataggtaatc ctagttgcga
     1501 ttcaacatct tcttctgtct taatacgctt atctaataat tcttttaaga aaataatcaa
     1561 tattgctaaa acaataccaa caataatgct gataactaag ttgacagata ctattggaga
     1621 tacttttaca gcattatcat gtgctgagga aagtatcgta acattatcaa cactcataat
     1681 tttaggcatg tcatgagcaa aaactttaga tattttatta acaattttgt cagattcaga
     1741 tttattccca gtggtaactg atacagtaat aatttgagag tttgtttgat tggttacttt
     1801 taaaaatgaa ttcaactcag ctgttgaata ctgaccatca aattctctag atactttatc
     1861 tagaattcta ggacttttga taatttccgt atatgtatta acagactgca aactactttg
     1921 aacattttgg aaagctaaat cacttgagga ctttttcatg ttcactaata tttgagtaga
     1981 agcagtatat ttgtcaggca taacaaaaaa ggttaatgcc gcacttacta caagacatat
     2041 tgccggtaaa ataagcaata atttaatatt cttctttaga atatttaata gttttactaa
     2101 atcaaacttt tctttcatgg tttcctccac ataatcaatc attgtattca ttatgtatgt
     2161 tttataaatc ggacaattat atctagttta acgaccacaa aacatacaca actacatttt
     2221 ctctaattat ttatataaat attttatcgt ttaaaattat atcatgattc tctaccatta
     2281 tgtataactt atttatattt ttgtacaaga tataatattg tccaacttta aatatccaaa
     2341 cctattaata ataaaactag ataccatcgt actctatcat ggctttctta taatcgagta
     2401 gaagcatcat cattacttga ttatttgctc tttacaacac cgagcgtgcc cgtactcggt
     2461 aattcaatac cttgcgtaac ccgtcactgt gagttgggtt aatgataata aagcccacac
     2521 cttttaaaaa gatgtgggta atttatataa tttttattta catttttaac ttataaaaaa
     2581 agcgcctatg tcatgattta ccatcacata ggcgcttatc aataaattat tacttattac
     2641 tttccatttc atctaattta tgcggatttc ctgtaattag atgacaactt attcttttca
     2701 ggggaacatt acacttttat aatatgttca aagacaaact taaccattca caaatataaa
     2761 gaataatatt atcaaatca
//
LOCUS       DAKTIP010000251         2760 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.256, whole genome shotgun sequence.
ACCESSION   DAKTIP010000251 DAKTIP010000000
VERSION     DAKTIP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.256"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            77..1246
                     /locus_tag="O0305_002595"
     CDS             77..1246
                     /locus_tag="O0305_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCY0275696.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(1353..1781)
                     /locus_tag="O0305_002596"
     CDS             complement(1353..1781)
                     /locus_tag="O0305_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCY0275697.1"
                     /translation="MILNFNQFENQNFFNANPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(1997..2443)
                     /locus_tag="O0305_002597"
     CDS             complement(1997..2443)
                     /locus_tag="O0305_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0275698.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
BASE COUNT          902 a          375 c          460 g         1023 t
ORIGIN      
        1 taacattgac ctctaatata taggcagagt attgatatct attaaaaaat aaatgatttt
       61 gatgaaggtg aaacgtatgt acaaaacaaa aggtggcttt caacttacat tacaaacatt
      121 aagtttagtg gttgggttta tggcttggag tataattgcg ccattaatgc cctttattaa
      181 acaagatgtc aatgttactg aaggtcaaat atcaatcatt ttagcgatac cagttatttt
      241 gggatcggtg ctccgtgtgc catttggtta tttaacaaac attgttggcg ctaaatgggt
      301 attctttact agttttatcg tattgttatt cccgatattt ttcttaagcc aagcacaaac
      361 accgggtatg ttaatggctt caggattttt ccttggtgta ggtggtgcaa ttttctcagt
      421 tggtgttaca tcagttccta aatatttccc taaagaaaaa gtaggtctag caaatggtat
      481 ttatggtatg ggaaatatcg gtacagcagt ttcttcattt ttagcaccac cgatagcggg
      541 tattattggt tggcaaacaa cagttagaag ttacttaatt attatcgctt tatttgcatt
      601 aattatgttc atttttggtg acacacaaga acgtaaaatt aaagtaccat taatggctca
      661 aatgaaaaca ttatctaaaa actacaaatt atattactta agttattggt attttattac
      721 ttttggtgct tttgtagcat ttggtatttt cttacctaac tacttagtaa atcattttgg
      781 aattgacaaa gtagatgctg gtattcgatc aggtgtattc attgcgctgg caacattctt
      841 aagaccaata ggtggcattt taggtgataa atttaatgca gttaaagtat tgatgattga
      901 ttttgttgtt atgattatcg gtgccattat tttaggtatt tcagaccata tcgcattatt
      961 cactgtaggc tgtttaacaa taagtatttg tgcaggtatt ggtaacggct taatcttcaa
     1021 attagtacca tcatacttct taaatgaagc gggatccgca aatggtatcg tatcaatgat
     1081 gggtggttta ggaggattct tcccaccact agtaatcacg tacgtagcta atttaacagg
     1141 atcaagtcat ttagcattta ttttcttagc ggtatttgga tgtattgcat tatttaccat
     1201 gcgtcattta taccaaaaag aatatggctc attgaaaaac ggttgatatg taatacatgc
     1261 cattcattta gttaaataca aagccttaat atcatgcgca atattcgtag catgacatta
     1321 aggctttagt agtgtattca ttttaaattt aactatgaaa ttggaataga tgttgatgaa
     1381 gtagtttcat tgcttggttt gattttaggc aaggtaatgg ttaataaccc attttcaaaa
     1441 ctagcagtaa tatgttgctt atcaacagct tcaaaatcaa attgacgcat taatgattcg
     1501 aagttacgct catctaaaat gagttgttca gatttgtatt ttgcgcttct agtagcttga
     1561 atagtgagcg tattattatt gaaatcgata ctaatatctt ctttatttac acctgctagt
     1621 tcagcttcta agtaatataa ttcgtctgtt tcatatatat tcgttacaaa tgaaggtgtt
     1681 gaaaagtaat taaatacttg tttacctaaa tctttaaatg tatcacttgg attagcgtta
     1741 aaaaagtttt gattctcgaa ttgattgaag ttcaatatca tcacttccat tatagaaatt
     1801 tgtattacaa agccattata tgagtagtat taaaaaagtt aaagtaaaag gggagataaa
     1861 aaagtaataa atgggtagat ttgtgcctga taaggaatca gggcaaaaga actgagcatc
     1921 gagatttata caagcaataa agataatgaa taaacatatc gatgcatact tctgcccatc
     1981 accttatgat agggagttat tttttgtcgg aatagtcaaa atttttagaa acaaaattcc
     2041 gcaaattatt aattatatct gatgcttctc gttctgaaat gttgaattca ttaaagacct
     2101 taacagaaat ttcagcaaga gggcttttta ttgctttacc ttgttctgtt agagaaattt
     2161 gtaggtttct ttcatctttc tcttcacgtg ttcgaacaac gtaatctttc ttttctaatt
     2221 tctttagtaa tggtgtcagt gttccagaat ctaagaacac acgttcacct aattttttga
     2281 tgttaagttt ttcatcattt tcaatcgcca ttaaaacaat gtaaccagta taggttaaat
     2341 catattcctt aagatagttt gtgtattttt taattatttc ttttgaagaa acataaaata
     2401 aaaaacacaa ctgtttgcta agatagctgt tttctacata catccaatca ctccttgtta
     2461 aaataaacaa tattatgaag tgtaatagtt gtcaagtact gatttcatcg caattattga
     2521 attgtctata attgaattta aatgttaaat aatgcaaaat atatcgatat cttagtattt
     2581 tcatcggtaa ttactgatat gactgatttt ttaaattgaa aaaaatgtaa ttgtatcatc
     2641 cttaattgtt aaaagtagtc aattattttg atagatagta aaataaaatt tgttattaaa
     2701 ttttttaaaa gcatcgtgta tgtaattcga tttctataat aatattgaga ataaaaaaat
//
LOCUS       DAKTIP010000252         2745 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.257, whole genome shotgun sequence.
ACCESSION   DAKTIP010000252 DAKTIP010000000
VERSION     DAKTIP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.257"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            151..615
                     /gene="accB"
                     /locus_tag="O0305_002598"
     CDS             151..615
                     /gene="accB"
                     /locus_tag="O0305_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCY0275699.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQAQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            615..1970
                     /gene="accC"
                     /locus_tag="O0305_002599"
     CDS             615..1970
                     /gene="accC"
                     /locus_tag="O0305_002599"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCY0275700.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            1985..2347
                     /locus_tag="O0305_002600"
     CDS             1985..2347
                     /locus_tag="O0305_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0275701.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            2407..>2745
                     /gene="nusB"
                     /locus_tag="O0305_002601"
     CDS             2407..>2745
                     /gene="nusB"
                     /locus_tag="O0305_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCY0275702.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSD"
BASE COUNT         1015 a          406 c          534 g          790 t
ORIGIN      
        1 tacacaagtg tttttaattt aaaatgtggt tttattataa ctttgggata gacttataat
       61 gtcgccagca atatgaacat gcttgaattg aagagttgtc tcaagtaaaa tagacgggta
      121 gatgaaaaca aactgaagga gtcagtaata atgaacttta aagaaatcaa agaattaatt
      181 gaaattctgg ataaatcaac tttaacggaa atcaatattg aagatactaa aggcaaagtg
      241 acgcttaaga aagaaaaaga aactgagatt atcacgccac aaatctcaca aatgccagtt
      301 gaagctgcgg caatgcctat gcctcaagca caatcaactg atagcaataa aactgaagct
      361 ccaaagccaa cttcagataa tcacaaaaca attaatgcac ctatggtagg tacattttac
      421 aaatcgccat ctccagacga agaagcatat gtgcaagttg gggacactgt ttcaaatgaa
      481 acaacagtgt gtattttaga ggcaatgaaa ctatttaatg aaattcaagc agaaatttca
      541 ggtgaaattg ttgaaatctt agtagaagac ggacaaatgg tagagtatgg ccaaccgtta
      601 tttaaggtga aataatgaaa aaggttttaa ttgcaaaccg cggtgaaatc gcagttagga
      661 ttattcgcgc ttgtcgtgat ttaggcatcc aaactgttgc aatctattct gaaggggata
      721 aagatgcgct acatactcaa attgctgatg aagcatattg cgtaggtccc actttgtcta
      781 aagattcata tttaaatatt ccgaacatct tatctattgc aacttctaca ggttgtgatg
      841 gcgttcatcc gggttatggc tttttagctg aaaatgctga ttttgcagaa ttatgcgaag
      901 catgccaatt gaagttcatt ggaccaagtt atcaatctat ccaaaaaatg ggtatcaaag
      961 atgttgctaa ggcagaaatg atcaaagcca atgttccagt tgttcctggt agtgacggtt
     1021 taatgaaaga cgtctcagaa gctaagaaaa tcgccaaaaa aattggctat ccggtcatca
     1081 ttaaagctac tgctggcggt ggcggaaaag gtatccgtgt tgctcgtgat gaaaaagaac
     1141 ttgaaactgg cttccgaatg acagaacaag aagctcaaac tgcatttggt aatggtggac
     1201 tttatatgga gaaattcatc gaaaacttcc gccatattga aatccaaatt gttggggaca
     1261 gctatggtaa tgtaattcat ttaggagaac gtgattgtac aattcaaaga cgtatgcaga
     1321 aattagtgga agaagcacct tccccaattt tagatgatga aacacgtcgt gaaatgggaa
     1381 atgccgcagt tcgtgcagcg aaagctgtaa attatgaaaa tgcgggaaca attgagttta
     1441 tatatgattt aaatgataat aaattttatt ttatggaaat gaatacacgt attcaagtag
     1501 aacatcctgt aactgaaatg gtaacaggaa ttgatttagt taaattacaa ttacaagttg
     1561 ctatgggtga cgtgttaccg tataaacaag aagatattaa attaacagga cacgcaattg
     1621 aatttagaat taatgctgaa aatccttaca agaactttat gccatcacca ggtaaaattg
     1681 agcaatatct tgcaccaggt ggatatggtg ttcgaataga gtcagcatgt tatactaatt
     1741 atacgatacc gccatattat gattcgatgg tagcgaaatt aatcatacat gaaccgacac
     1801 gagatgaagc gattatggct ggcattcgtg cactaagtga atttgtggtt cttggtattg
     1861 atacaactat tccattccat attaaattat tgaataacga tatatttaga agcggtaaat
     1921 ttaatacaaa ctttttagag caaaatagca ttatgaatga tgaaggttaa taggaggtca
     1981 atccatggtc aaagtaactg attattcaaa ttcaaaatta ggtaaagtag aaatagcgcc
     2041 agaagtgcta tctgttattg caagtatagc tacttcggaa gtcgaaggca tcactggcca
     2101 ttttgctgaa ttaaaagaaa caaatttaga aaaagttagt cgtaaaaatt taagccgtga
     2161 tttaaaaatc gagagtaaag aagatggcat atatatagat gtatattgtg cattaaaaca
     2221 tggtgttaat atttcaaaaa ctgcaaacaa aattcaaacg tcaattttta attcaatttc
     2281 taatatgaca gcgatagaac ctaagcaaat taatattcac attacacaaa tcgttattga
     2341 aaagtaatgt catacctaat tcagtaatta aataaagaaa aatacaaacg tttgaaggag
     2401 ttaaaaatga gtcgtaaaga atcccgagtg caagcttttc aaactttatt tcaattagaa
     2461 atgaaggaca gtgatttaac gataaatgaa gcgataagct ttattaaaga cgataatcca
     2521 gatttagact tcgaatttat tcattggcta gtttctggcg ttaaaggtca cgaacctgta
     2581 ttagacgaga caattagtcc ttatttaaaa gattggacta ttgcacgttt attaaaaacg
     2641 gatcgtatta ttttaagaat ggcaacatat gaaatattac acagtgatac acctgctaaa
     2701 gtcgtaatga atgaagcagt tgaattaaca aaacaattca gtgat
//
LOCUS       DAKTIP010000253         2675 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.258, whole genome shotgun sequence.
ACCESSION   DAKTIP010000253 DAKTIP010000000
VERSION     DAKTIP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.258"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            322..990
                     /gene="queC"
                     /locus_tag="O0305_002602"
     CDS             322..990
                     /gene="queC"
                     /locus_tag="O0305_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCY0275703.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            992..1411
                     /gene="queD"
                     /locus_tag="O0305_002603"
     CDS             992..1411
                     /gene="queD"
                     /locus_tag="O0305_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCY0275704.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1415..2128
                     /gene="queE"
                     /locus_tag="O0305_002604"
     CDS             1415..2128
                     /gene="queE"
                     /locus_tag="O0305_002604"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCY0275705.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2227..>2675
                     /locus_tag="O0305_002605"
     CDS             2227..>2675
                     /locus_tag="O0305_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275706.1"
                     /translation="MIVYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKL"
BASE COUNT          947 a          426 c          434 g          868 t
ORIGIN      
        1 tttataatat gtattggatg tagctaaaac tttaaaagca ttgtcattat cctgaccttg
       61 attttaacta atatatggta tattctattc atcgtacata aatgaatatc agaggttcct
      121 agctgaaacc ctctataaaa aactagacat tgaaatttca aacattcgtt ggtaaacctt
      181 taatatatat tataggagca acccatttct ttcgtctatc ttttatggag ataggctttt
      241 tttattttat aaattaaaaa cgggcttatc aatgccatta acctctttat taatattatt
      301 aatgatagga gcaattacaa aatggaaagt gtattaaata atgaaaaagc catcgttgtc
      361 tttagtggtg gtcaagacag tacaacatgt ctcttttatg caaaaaaaca tttcaaagaa
      421 gttgaactcg taacgtttaa ttatggccaa agacatgata ctgaaattga agtcgcaaaa
      481 caaattgcac aagatcaagg aatgaaacat cacgttttag atatgtcatt attatcacaa
      541 cttactccaa acgcattaac acaacatgat atggaaatta ctaataatga agatggtata
      601 cctaatacat ttgttccagc tagaaattta cttttcttgt cgtttgcagg cgctctagct
      661 tatcaaattg gggctaagca tattattaca ggcgtatgtg aaacagactt ttcaggctac
      721 ccagactgtc gcgatagttt tataaaatca atgaacgtaa cattaagcct agctatggac
      781 aaagattttg tcattcatac tcctttaatg tggttaaaca aagcagaaac gtggaaatta
      841 agtgatgaac tcgaagtttt agattatatt cgtacaaaaa cattaacatg ctataacggt
      901 atcattgggg atggctgtgg tgaatgtcca gcttgtcatt tacgtcaacg tggactaaat
      961 caatatcttg aaagtaaagg agcgctttaa aatgttacaa caaatctatc ctagtacaac
     1021 gcatccatat caattcgaat taaataaaga ttttaatttt tcggctgcac atcacattcc
     1081 atgtgaagaa gcaggtattt gtcaaaatgt ccatggtcat acttactttg ttaatttaac
     1141 aattgtcggt gataaactag atgacactgg cttcttagtg aactttagcc atttgaaaaa
     1201 gatgatacac ggtaaatttg accatcaact gttaaataac ttacctgctt ttaaaaacaa
     1261 aatcccttca actgaaatcg tagcggaaac aatttatcaa attgttaaag aaaatttggc
     1321 atcgctcgaa caccaaccaa aatgtattca agtatttgta agagaaacac caacaagtta
     1381 tgtcgtattt agaccaaagg aacaggtgta actaatggct aaaattcctg tactcgaaat
     1441 attcggtcca accattcagg gtgaaggtcg agtaatcggt agaaaaacga tgtttgtgag
     1501 aacagctgga tgtgattatc gctgtagctg gtgtgattct gcatttactt gggatggtag
     1561 tgctaaaggc gatataaaac tcatgaccgc tgaagaaatt tatgatgaat taaaacgaat
     1621 tggtggcgat ttatttaacc acgttacaat ttctggtggt aacccagcat taattaaagg
     1681 tatccaagag ttagttgact tatttcaaga taaaggtatt ttcagcgcac tggagacaca
     1741 aggcagtaaa ttccaacctt ggatgacaca aattgatgat ttaacgatta gtccaaaacc
     1801 accaagttca actatgacac ctgatttaaa aaagttagat gaagtgatta cacaatgcgt
     1861 accttcatca ttaaacttaa aagtagttgt attcgacgat aaagattatg attttgccaa
     1921 aatgatacac caccgttatc cagatattcc cttctattta caagttggta acccatattt
     1981 atcagacagc gtagataatc ataccgaaaa gttgttagaa cgctacgagc agttggtgga
     2041 tttagttatg caaagtaatg atatgaatca cgtttatgtg ttaccacaac ttcatacatt
     2101 actttggagc aataaaaaag gtgtataaag gataacttat tttacttttt gcaagtcata
     2161 tctaatgaat aacaatatat taattgatat aatagttgat tgaagtataa cgaacaggag
     2221 tccatcatga ttgtatatat attaattaat attgctatcg tcgtacttat aactggattc
     2281 aatttatata ggcatcaaat gcaacattta tcattaagtg caatgttgtt atctattacg
     2341 ataaacgcct ttataaatac attcataatt gataaatata actttattac tctgtgtact
     2401 ataacgatgt ttatcatttg gacgatacta cagttttaca ttgataaaaa attaaaacct
     2461 gtatacatta cagaccaaaa atttattgct atcatattaa cgattgttgt cagtttaacg
     2521 caacgtgtta ccgacttttc aagtacacaa tcaatatata tgtctattcc atttttagcc
     2581 cctgccattt tcatcattgg tggtattatg ttgtttattt caacatttaa tagcttagat
     2641 gaaactgctg aaaataataa caaaataaag aaact
//
LOCUS       DAKTIP010000254         2656 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.259, whole genome shotgun sequence.
ACCESSION   DAKTIP010000254 DAKTIP010000000
VERSION     DAKTIP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.259"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            10..1347
                     /locus_tag="O0305_002606"
     CDS             10..1347
                     /locus_tag="O0305_002606"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCY0275707.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            1340..1570
                     /locus_tag="O0305_002607"
     CDS             1340..1570
                     /locus_tag="O0305_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCY0275708.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            1548..2429
                     /locus_tag="O0305_002608"
     CDS             1548..2429
                     /locus_tag="O0305_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCY0275709.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         1038 a          354 c          483 g          781 t
ORIGIN      
        1 tgatgttata tgtcagatta tttaagtgtt tcagctttaa cgaaatatat taaatataaa
       61 tttgatcaag atccacatct ccaatctgta ttgattaaag gtgagctttc aaatttcaaa
      121 aagcattcaa gtggtcattt atactttaat gtgaaagata aagaaagcgt tataagtgcc
      181 atgatgttca aaggtagtgc ttctaaatta aacttcgaac ctaaagaagg agatgaagtc
      241 ttattagaag cacgtgtttc tgtttttgaa cgtcgtggaa actatcaaat ttatgtaaat
      301 aaaatgcaat tagatggtat agggaactta tatcaaaaat tagaagcatt aaagaaaaaa
      361 ttaactgaag aaggttgttt tgataaagcg aataagaaat caatacctaa gtttcctaaa
      421 aaaattgcag ttttaacagc gagtacaggt gccgcaattc gagatatcca ttcaacgata
      481 aatagtcgtt ttccattagc tgaacaaata caaattagta ctttggttca aggtgaaaaa
      541 gcaaaagacg acattattga aaaaattgaa tatgcagata gtttaggtgt agataccatt
      601 attgtaggtc gaggcggtgg atccatagaa gatttatgga atttcaacga agaagctgtc
      661 gtccgtgcaa tatataattg taagacaccg attatatcag cagttggtca tgaaacagac
      721 tttacattaa gtgattttgc tgcagacatc agagctgcga ctccaactca agctgctgtt
      781 attgcaacac cagaccaata tgaattgctg caacaaatac agcaatatca gtttacattg
      841 actcgtttca tcaagaaaca tttagaacaa caacgtaaac atgttgaaca tttgtcatcg
      901 tattacaagt ttaaacaacc aactttattg tatgatcaac agatacaacg tcgagatgat
      961 ttagaaaaga gactgaaaca acaaattcag gcaacttttg aacaacagag acatcgatta
     1021 atgctgttgc aacaacgtta taatttaaaa gcattactaa gtagtgttaa tcaagaacaa
     1081 caaaataatc ttcaattgac aaaccaactt gttaaattat tgaattctaa gatattaagt
     1141 tataaaaatg atttgaaaaa taaagtagaa aacttaaaca atttaagtcc aactaataca
     1201 atgttgcgtg gatatgcaat tgttaataaa aaagacgaag tcattacgag tactaaagat
     1261 ttaacagaaa atgatcaatt gacattaaca atgaaagatg gcttagtaga tgcaaaagtt
     1321 acgaaagtaa ggtgtaataa tgactaaaga aacgcaaagt tttgaagaaa tgatgcaaga
     1381 attagagcaa attgttcaaa aattagataa tgaaacagta tctttagagg aatcattaga
     1441 tttatatcaa cgtggtatga aactatcagc agcttgtgac acaactttaa aaaatgccga
     1501 aaaaaaggtg aatgacttaa taaaagaaga agctgaggat gtaaaaaatg acgaatctac
     1561 cgatgaataa attaatagat gaagtcaata atgaattatc ggttgcgata aataaatcag
     1621 taatggatac tcagctagaa gaaagtatgt tgtattcatt aaatgctgga ggtaaacgca
     1681 tccgaccagt tctgttatta ctcactttag attcactaaa taccgagtat gagttaggta
     1741 tgaagagcgc aattgcacta gaaatgattc atacatattc acttattcat gatgacctac
     1801 cagcgatgga taatgatgat tatcgacgag gaaaattaac aaatcataaa gtatatggtg
     1861 agtggactgc gatattagca ggtgatgctt tattaactaa agcatttgaa cttatttcaa
     1921 gtgatgatag attaactgat gaagtaaaaa taaaagttct acaacggctg tcaatagcaa
     1981 gtggtcatgt tggaatggtc ggcggtcaaa tgttagatat gcaaagcgaa ggccaaccaa
     2041 ttgatcttga aactttggaa atgatacaca aaacaaaaac aggagcatta ttaacttttg
     2101 cggttatgag tgcagcagat atcgctaatg tcgatgatac aactaaagaa catttagaaa
     2161 gttatagtta tcatttaggt atgatgttcc agattaaaga tgatttatta gactgctatg
     2221 gtgatgaagc aaagttaggt aaaaaagtgg gcagcgatct tgaaaataat aaaagtacgt
     2281 acgtgagttt attagggaaa gatggcgcag aagataaatt gacttatcat agagacgcag
     2341 cagtggatga actaacgcaa attgatgaac aattcaatac aaaacactta ttagaaatcg
     2401 ttgatttatt ttatagcagg gatcactaaa agcaaatata tcgattagca acaattgtaa
     2461 agtttgaaat gtctcaaaag gcatattcaa ctgacgttgt ttgttaatcg atttttctca
     2521 tgttgaaact taaaattatg ttgaagactt tcatttaaaa aatagaattt tcaatttcta
     2581 tacgacaata ttcgtatctg tgatatcaat gtgattttaa ttaaatatgt tcaaacaatt
     2641 gacgcttatt aaatct
//
LOCUS       DAKTIP010000255         2637 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.260, whole genome shotgun sequence.
ACCESSION   DAKTIP010000255 DAKTIP010000000
VERSION     DAKTIP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.260"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            46..1527
                     /gene="cls"
                     /locus_tag="O0305_002609"
     CDS             46..1527
                     /gene="cls"
                     /locus_tag="O0305_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCY0275710.1"
                     /translation="MRFTFSNDLGTLFTIILAIGFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDGLLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKVDHFIDGNDLYDQVLKDIKNAKEYIHLEY
                     YTFALDGLGKRILHALEEKLKQGLEVKILYDDVGSKNVKMANFDHFKSLGGEVEAFFA
                     SKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGKLGYWRDTHLRIQGDAV
                     DALQLRFILDWNSQAHRPQFEYDVKYFPKKNGPLGNSPIQIAASGPASDWHQIEYGYT
                     KMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIPCKPDHPLVYWATFSNA
                     SDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSFELNFEVNAFVYDENLA
                     KDLRVAYEHDITKSKQLTKESYANRPLSVKFKESLAKLVSPIL"
     gene            1702..2610
                     /locus_tag="O0305_002610"
     CDS             1702..2610
                     /locus_tag="O0305_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275711.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFWSIIENLKEDNRTILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQS
                     QITLSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKV
                     SIVDSYFNNKKQRGSNYDTKLLENRI"
BASE COUNT          987 a          330 c          431 g          889 t
ORIGIN      
        1 ggtgcaaaat gtttttttgt gcctttcttt ataggagaaa agactatgcg atttacattt
       61 tcaaacgatt taggaacgtt atttactatt attttagcca ttggattcat cattaattta
      121 gtattggctt ttattattat ctttttagaa agaaataggc gtacagcgag ttcaacttgg
      181 gcatggctat ttgtactttt tgtcttacca ttgattggtt ttattcttta cttgtttttt
      241 ggtagaaccg tttcggcacg caaattgaat aaaaacaatg gtaacgtgtt aacggatttc
      301 gatggacttt taaaacaaca aatagaaagc tttgataaag gtaattatgg tactgataac
      361 aaacaagttc aaaaacatca tgatttagta cgtatgcttt tgatggatca agatggtttt
      421 ttaactgaaa ataataaagt tgatcatttc attgatggaa atgatttata tgatcaagtt
      481 ttaaaagata ttaaaaatgc aaaagaatat atccatttag agtactatac tttcgcttta
      541 gatggtttag gtaaaagaat tttacatgct ttagaagaaa aattgaaaca aggtctagaa
      601 gtaaaaatat tatatgatga tgttggatct aaaaatgtta agatggcaaa ttttgatcat
      661 tttaaatcgt taggtggaga agttgaagca ttttttgctt caaaattacc gttattgaat
      721 ttcagaatga ataatagaaa tcatagaaaa atcatcgtaa tcgatggtca actaggttat
      781 gtcggaggat ttaacattgg tgatgaatat ctaggattag gaaaattagg atattggaga
      841 gatacgcatt tacgtataca aggggatgcg gttgatgcac tgcagttgcg atttatttta
      901 gactggaatt cgcaagcgca ccgtccacaa tttgaatatg atgttaagta tttccctaaa
      961 aagaacggac cattgggcaa ttcaccaatt caaatagctg caagtggccc ggctagtgac
     1021 tggcatcaaa ttgaatacgg ttatacaaaa atgattatga gtgcaaagaa atctgtatat
     1081 ttacaatcac catatttcat tccggataat tcatatataa atgccattaa aattgctgct
     1141 aaatcaggtg tagatgtaca tttaatgatt ccatgtaagc cagatcatcc attagtatat
     1201 tgggcgacat tttcaaatgc ctctgactta ttatcaagtg gtgttaaaat ttatacgtat
     1261 gaaaatggat ttatacattc taaaatgtgc ttaattgatg atgaaatcgt atcagtgggc
     1321 acagcaaata tggactttag aagttttgaa ttaaattttg aagtaaatgc ctttgtatat
     1381 gatgaaaatc ttgctaaaga tttaagggtg gcttatgaac atgatattac aaaatcaaaa
     1441 caactaacca aagaatcata tgccaataga ccgctgtctg ttaaattcaa agaatcgtta
     1501 gcaaaattag tttcgccaat tttataattt atttgtaagg agtctcgatt atagaggctc
     1561 tttttatttt gcccaaaatt aaaataacga taaatatatt atgtaaatct aataatgaca
     1621 tttgtcattt caaatctatg acaaagtgtt ctattttaaa tctaataaat ttactaatat
     1681 taaattaaga aaaaaataga ggtgattaaa ttgattcaaa tatctaatat caacaagtca
     1741 tttaataaaa gatgtgttct aaaaaatatt tcgttcgata ttgaacaagg taaatgtatc
     1801 gctttaattg gaaaaaatgg tgctggaaag tcaacgttaa ttgatatatt aattggtaat
     1861 gttaatgcta attctggtga gatatttgat aaagacaagt tattacaaag tgaaaatcgc
     1921 agtataatgt tccaaaaaac gatgtttcca gatcaattaa aagttattga gattatcaac
     1981 ttatatcaat cattttacga aaatccatta ccattggaag aaataataga actgacgaaa
     2041 tttgattcta gtcaactgaa ccaatttgta aataaacttt ctggtggtca acaacgatta
     2101 ctcgattttg tattatcttt aatcggacaa ccacaattga tcttattaga tgaaccaaca
     2161 tcgactatgg atatagaaat tagagaatat ttttggtcaa ttattgaaaa tttaaaagaa
     2221 gataatcgaa cgatactcta tacatcgcac tatattgaag aagtcgaacg tatgtcagac
     2281 aaaattattc tcattgaaaa tggagaaata atacttaatg attcaacgtc acatattaga
     2341 accaatcagc aatctcagat tacgttatcc gatgaatata taagaaagtt aaaactagat
     2401 aaagatgatt tagttattca aaaaaatcat aatggcacta tcaaaattat tacttcaaat
     2461 gtaaatgata cgattttata tcttcaacaa cttcatatta atttggatga tattgaaata
     2521 caaaaagtct caattgttga ttcatacttc aacaataaaa agcaaagggg atctaattat
     2581 gatactaagt tacttgaaaa tcgaatttaa agttataatg cgtaaaaaaa caacatt
//
LOCUS       DAKTIP010000256         2625 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.261, whole genome shotgun sequence.
ACCESSION   DAKTIP010000256 DAKTIP010000000
VERSION     DAKTIP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.261"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..513)
                     /locus_tag="O0305_002611"
     CDS             complement(<1..513)
                     /locus_tag="O0305_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275712.1"
                     /translation="MLLCDVRVIYKNPKYKVIQHNGEYLLVDLVSTWFVYFFPFINWF
                     IPKKYAIISEEEFENLNVVKPNKNNVFWSVIGSSVLFGVTLRKYIHVFDVQLDKLVVM
                     ILCALALICVIVFYFNLNRKLKLKVFDTNIEKNKRVILIPTFKLGCFLVFGYIFAGSF
                     SIFSLIALMTI"
     gene            complement(966..1595)
                     /locus_tag="O0305_002612"
                     /pseudo
     CDS             complement(966..1595)
                     /locus_tag="O0305_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837136.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
     gene            complement(1642..2283)
                     /locus_tag="O0305_002613"
     CDS             complement(1642..2283)
                     /locus_tag="O0305_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0275713.1"
                     /translation="METLLCESKVINKNPKYRIIKYDSEYLMIDLASNWIVFFFPFIN
                     WLIPKTYVKITKNDYEKLNIVKPVKNKSIGWTIFAGIVLLGGTVRRNTYLFDFQLEEL
                     IVWSSCFIGFLEIIFFYCYLNKKLTLNIYNESKNNELKLRLLPSFKNICFTIFYYLFT
                     GFMSYGAFYLLVFENVQNLILYVSWLFMTMLFMFMNMHSIIDKKVHIFLKSNK"
     gene            complement(2414..>2625)
                     /locus_tag="O0305_002614"
     CDS             complement(2414..>2625)
                     /locus_tag="O0305_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275714.1"
                     /translation="LIPTLKNFCLTLFRYAFFILWTVIFSYALLSMSYQNIIVYFAWI
                     TAIMGFFLVNIALIIDKNIHVILKN"
BASE COUNT          998 a          417 c          277 g          933 t
ORIGIN      
        1 gattgtcata agggcaatta atgaaaatat tgaaaaactt ccagcgaaaa tatatccgaa
       61 aactaaaaaa cagccaagtt taaacgttgg tattaatata actctcttat ttttttcaat
      121 atttgtatca aacactttta acttaagctt tctatttaag ttaaaataaa aaactataac
      181 acaaattaaa gcgagagcac acaatatcat tacaactagc ttatctaatt gaacatcaaa
      241 aacatgtatg tatttcctta aagtaactcc aaacaaaacc gaacttccta taactgacca
      301 gaaaacatta tttttatttg gtttaacaac atttaaattt tcaaattctt cttcgctaat
      361 tatcgcgtac ttttttggaa tgaaccaatt aatgaaagga aaaaagtaca cgaaccaagt
      421 gcttactaaa tcgactaata agtattcacc gttatgttga atgactttgt atttcggatt
      481 tttataaatg actctgacat cgcaaagcaa catttaccac tcccgtttca ttagttttaa
      541 atgatcccca atactttttg taccttcacc aactatcgtt ccaacactat ctacagcttt
      601 ttctgtggta tttttaaaca gcgtctattt aatgtcttat ccaattagtt ttatctctta
      661 atccaaattt attagtatga aaagtccaat ttttaagaat tgtctaaaca gattccgcaa
      721 caaaaccagc taaaaccgtc catactattg gattaaaaac gccaatgtta taagagctat
      781 catacaaaat aacacgctct atatttcatt tttaaattaa aacagttttt tcctttatca
      841 atcacaatgg gcattttcac ttggtatatt caaacattac tatctatttt cctgatttaa
      901 gatatgatta tatttttttg aaccaccgtt taaatatgcg atactaccaa tttcgatcat
      961 ttttactact cgtttattct taaaataaca tgaacatttt tattacctat cgaagccata
     1021 ttcactaaaa agaaaaacat cgtgataata acccaagtta aaaatattat tatattttgt
     1081 gactctgtcg tcaaaagaat ggttaaagta aatattgaag cgcttcctaa aaaaaagtac
     1141 gcaaaaacta ttaacaatcc ttgtttgaaa gtcggtatta atacaactct attcttatta
     1201 accacgttgg tattaaaaat ttttaatgtt aattttttat ttagataaaa ataaaaaatt
     1261 aacatcccta ttaacatgat aaaaaataca gtgattgcta gttgtttttc aaactgaaca
     1321 ttaaagaagt taccgtactt tctcaatata attccaaata gaactgaact tcctgcaacc
     1381 ggccaaaaaa cattattttt aacaggctcg actatattta acctttaaaa ttcattttcg
     1441 ctaatttttg cgtatttttt gggcaaaaac caattaatca tagggaaaaa ataagtaatc
     1501 caagtactta ctaaatcgac catgaaatat tcattattat atctaataac tcgatattta
     1561 ggatttttat aaatttgtct agattcacaa agcactgttt accaccccta attaacaatt
     1621 gaaaactaac taaatttgta actatttatt agactttaag aatatatgta cttttttatc
     1681 tataattgaa tgcatattca taaacataaa tagcatagtc atgaaaagcc aagaaacata
     1741 taagattaaa ttttgcacat tttcaaatac caacaagtaa aatgccccat aagacatgaa
     1801 accagtaaat aggtaataaa aaattgtgaa acaaatattt ttaaaggagg gtaataatct
     1861 taatttaagt tcattatttt tactttcatt ataaatattt aatgttaatt tcttatttag
     1921 ataacaataa aaaaatataa tctctaaaaa cccaatgaaa cagctgctcc aaacaattag
     1981 ttcttctaat tgaaaatcaa ataaataagt atttcttctt acagtaccac caagtaacac
     2041 aatacccgcg aatatggtcc atcctatcga tttattttta actggtttga caatatttaa
     2101 tttttcataa tcattcttag tgattttgac atatgttttc ggtatgagcc agttaataaa
     2161 tggaaagaag aagacaatcc aattacttgc caaatcaatc attaaatatt cactatcgta
     2221 tttgattatt cgatatttag ggtttttatt aataacttta gattcgcaaa gcaatgtctc
     2281 cacatccctt taattttatg tgtaatacat ttttcgatac ttcaaaagac attcaaatac
     2341 tatcaagtta ctgtcatcaa aggttttatt aactgatatt ttcatatttt taatctgaat
     2401 ttagtacttt cacttaattt ttaagtatga catgaatgtt tttatctata attaaagcta
     2461 tattcactag aaaaaaaccc attattgctg taatccaagc aaaatatact attatgtttt
     2521 gataactcat tgataataga gcatatgaga aaatcactgt ccataaaata aaaaaagcat
     2581 atctaaacaa tgttaaacaa aaatttttta gtgttggtat taaga
//
LOCUS       DAKTIP010000257         2622 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.262, whole genome shotgun sequence.
ACCESSION   DAKTIP010000257 DAKTIP010000000
VERSION     DAKTIP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.262"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            138..845
                     /gene="deoD"
                     /locus_tag="O0305_002615"
     CDS             138..845
                     /gene="deoD"
                     /locus_tag="O0305_002615"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0275715.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            852..2204
                     /gene="tet(38)"
                     /locus_tag="O0305_002616"
     CDS             852..2204
                     /gene="tet(38)"
                     /locus_tag="O0305_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0275716.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
     gene            2285..>2622
                     /gene="deoC"
                     /locus_tag="O0305_002617"
     CDS             2285..>2622
                     /gene="deoC"
                     /locus_tag="O0305_002617"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0275717.1"
                     /translation="MKFEKYIDHTLLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
BASE COUNT          845 a          392 c          493 g          892 t
ORIGIN      
        1 aaggttagta gctgtaactg taaaatgttc ttaatattgt caaaatgtaa tgcttgaaag
       61 cgcttttaaa aaatattatt atatacatgg ttagacaaat agacaaatca ctatacaaat
      121 attgggagga atattttatg aaatcaacac cacacattaa accaatgaat gacgtcgaaa
      181 ttgcagaaac ggttctattg ccaggagatc cgttaagagc taagttcatt gcagaaactt
      241 atttggatga tgtggaacag ttcaatacag tgcgaaacat gtttggtttt accggaacat
      301 ataaaggtaa aaaagtttct gtcatgggtt caggtatggg tatgccatct attggcattt
      361 actcttatga attaattcat acatttggtt gtaaaaaatt aattcgcgtt ggctcttgtg
      421 gcgcgatgca agaaaacatt gatttatatg atgtgattat tgcacaaggt gcctctactg
      481 attcaaatta cgttcaacaa tatcaattac caggtcattt tgcgccaatt gcttcttatc
      541 aattattaga aaaagcagtt gaaacagcac gtgacaaagg tgtacgtcat catgtaggta
      601 atgtgttatc aagtgatatt ttctataacg cggatacaac agcgagtgaa cgttggatgc
      661 gtatgggtat tttaggtgta gaaatggaat cagctgcatt atacatgaat gcaatttacg
      721 ctggtgtcga agcattaggt gtgttcacag tgagcgatca tttaattcat gaaacgtcaa
      781 caacacctga ggaaagggaa cgtgcattta cagatatgat tgaaattgca ctgtcattgg
      841 tgtagatgat tatgaatgtt gaatattcta aaataaagaa agcagtacct attttattat
      901 tcttatttgt attcagtttg gttatagaca actcatttaa attgatttct gtagccattg
      961 ctgatgactt aaacatatct gtaacgacag taagttggca agcgacatta gccggtttag
     1021 taattggtat tggcgctgta gtatacgctt cattatctga tgccattagt atacgcacac
     1081 tatttattta tggcgtgata ttaatcatta tcggatcaat tattggttac attttccaac
     1141 atcaattccc attactttta gttggacgta ttattcaaac tgccggttta gctgctgcag
     1201 agacattata tgtgatatat gttgcaaagt atctttctaa agaggaccag aagacttacc
     1261 ttggcttaag tacgagcagt tattccttgt cattagttat cggtacatta tcaggtggat
     1321 ttatttctac gtatttacac tggacaaata tgtttttaat tgcattaatc gtagtattta
     1381 cgttgccatt cctatttaaa ttattaccaa aagaaaataa tacgaataaa gctcatttag
     1441 attttgttgg cttaattcta gtggcaacta ttgctacaac agtcatgctg tttattacga
     1501 actttaattg gttatatatg attggtgcct taattgcgat tatcgttttt gcgctatata
     1561 ttaaaaatgc gcaacgtcca ttagtaaata aatcattttt ccaaaataaa cgttatgctt
     1621 catttttatt tatagtattt gtaatgtatg ctatccaatt gggttatatt tttacgttcc
     1681 cattcataat ggagcaaatt tatcatctgc aactagacac aacatcactg ttattagtac
     1741 cgggttatat agtagcagtc attgttggtg cactaagtgg taaaatcggc gaatatctga
     1801 attcaaaaca agcgattatc acagcaatta ttttaatagc actgagcttg attttacctg
     1861 catttgcagt aggtaatcac atttcaatct tcgtcatttc tatgatattc tttgcaggta
     1921 gctttgcttt aatgtatgca cctttactta acgaagccat taaaacaata gatcttaata
     1981 tgacaggtgt ggctattggt ttttataatt taattattaa tgtggcggta tctgtaggta
     2041 ttgcgattgc tgcggctcta atcgatttta aagcattaaa tttcccaggc aatgatgcat
     2101 taagttcaca tttcggtatt attttaatta ttttaggttt aatgagtatt gtcggattag
     2161 ttttattcgt cagcttaaat cgttggacac aatctgaaaa ataaatagat ataaattcgc
     2221 gagatatatt cgtatttata gtaaaattaa ataaagagat tatataacac gaggagtagt
     2281 aagtatgaaa tttgagaaat atatagatca cactttattg aagcctgagt caacacgtac
     2341 gcaaatcgat caaatcatcg atgaagcgaa agcatacaat tttaaatctg tatgtgtgaa
     2401 tccaacacat gttaaatatg cagcagagcg actagctgat tcagaggtgc tcgtttgtac
     2461 ggtaatagga ttcccattag gtgcgtcgac aactgcaacg aaagcatttg aaacagaaga
     2521 tgcaattcaa aatggtgcag atgaaattga catggtcatc aacatcggcg cattaaaaga
     2581 tggacgtttt gatgatgtac aacaagacat tgaagcagtg gt
//
LOCUS       DAKTIP010000258         2617 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.263, whole genome shotgun sequence.
ACCESSION   DAKTIP010000258 DAKTIP010000000
VERSION     DAKTIP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.263"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..846
                     /locus_tag="O0305_002618"
     CDS             1..846
                     /locus_tag="O0305_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCY0275718.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSNILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(1218..2441)
                     /locus_tag="O0305_002619"
     CDS             complement(1218..2441)
                     /locus_tag="O0305_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCY0275719.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWSYPPFQLTEKDGKLYGRG
                     TTDMKGGLMALVVSLIELKEQNELPHGTIRLLATAGEEKEQEGAKLLADKGYLDDVDS
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNLFKEKYSE
                     LKQQDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDEIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
BASE COUNT          901 a          391 c          401 g          924 t
ORIGIN      
        1 atgaagcaac tggttggaat tcccgaatca atgttaattc ctttgatagc tcgagcaaaa
       61 gagtacgaaa acgaaaaacc aataataaaa gacgcactat caaaaaaaat atttgatggt
      121 ttagatgata tgtacaaaaa tgttacatgt gatgacatgt ctcaaattgg aattagtata
      181 cgtactgtga taatagattg tgttactaag aggcttatca aggataataa agatttaatc
      241 gtggtcaata taggttgtgg cttagataca aggtttcaaa gatttaataa agaaaaaata
      301 tcatggatag atttagatgt accagaatca atagaaatac gaaaaacatt ttttaaagaa
      361 agtaatagtt ataagatgat atctaaatct atgctagatt acagttggat tgatgatgtc
      421 aaaaattata aattttttaa tagtaagtca aatatattgt ttattattga aggtgtattg
      481 atgtattttg atgagagtgt aatgactcaa ttattggaca ctattatcaa aaagatggga
      541 gatcataatt tgacatttgc gattgaattt tgctcaaaaa caattgcgaa taatacaaag
      601 agacatcaat cggtatcaaa gttatcctca caacctgttt ttaaatatgg ttacaatgat
      661 ttaaaaaaat tgaatgaaat tttaccaaat acaataagag taatacatga atataattat
      721 tttgactatt ataagaatcg atggggctta tttggatatt gtagatttat accttattta
      781 aaaaaaaggt taaacaataa aatagtactt atgaaatata aagttccaaa gagacagaga
      841 cattaagttc ttagacaatg taaaaaagct gatttctata aattatttga tagaaatcag
      901 cttttttcaa atgtatttga taatttacag ctcgttgagc tgctgttttc tagggattta
      961 tgtcccattc cttttacgag aaggttttta cgagttttat gtaactatta gaagctatct
     1021 ttttatatga ccattagtca atatatgtat gacaaatcag cacaataatt tcagttttac
     1081 atcacttaat caatgaaaat gtgcttgttg atatttgaat atttacattc aaacaatatt
     1141 catcgctatt tctatcaact taaaattcgc aataaaaaac tgccagcacg cgaatagggc
     1201 agctgacagc atcgttctta tttttctttt aaatattgaa tggaagcctc tttaaaaata
     1261 tcaatatatt tcagatacat atctttttca atatattcat cgatttgatg tgccattaat
     1321 ggattacctg gtccaaaaat ggctaaatca acattgtcct tattatctcc taagaaacta
     1381 gaggcatctg ttgcacctac aagcgctgaa acaaatattt cgtcttgttc tacataacta
     1441 gaagctacat ctttaatcgt agtaattaat ttgctatttt tatcgcttgt tacaggtcgg
     1501 tgattgcttg gaatatcgag tgaaagctta ttgctatcca catcattaat gatattttgg
     1561 aaaaacgatt ctataaagtc gttatcatac tcaggaactg gtcttacgtt aaattcaagt
     1621 gaagcttcat ctggtacaga gttaaattgt ttgccgccat ttataatcga acatacagct
     1681 gtaagaccag atgcataatt tgcatcctct tcagaaattt cttttccaat caatgatttg
     1741 aacataggcg caacatctaa ttcatgttta gtatcttgtt gtttaagctc tgaatatttt
     1801 tctttaaata gattataaaa ttcaagcagt gtatcaattg cattgtcacc aataaatgga
     1861 actgagctat ggacagcttt accagttgca gttactttac atgacataga ccccttatgt
     1921 gcataataaa ttccagatcc agttggttca gcaataatta agctatcgac atcgtctaaa
     1981 tagcctttat cagctaataa tttggcacct tcttgttctt tctcttcgcc agcagtagcc
     2041 agtaatctaa tcgttccatg aggcaattca ttttgttctt ttaattcgat tagagatacg
     2101 accaaagcca ttaaaccgcc tttcatatct gtagtgcctc ggccatataa tttgccatct
     2161 ttttctgtca gttgaaaagg gggatatgac caattatctt gatttcctgc atcaacaaca
     2221 tccatatgac cactcaatgc gagtataggt gagccgttac cgatttctgc aacgatattg
     2281 gcgcggtgtt cattaacttt caaaatttca gatttaatat cgtacttgtc gaataaatct
     2341 gttaaataat tacaaacgtc tatttcatta ttattttcag tttgtagttc aacaatatct
     2401 gctagtaatt gaattttttc tttttcacta aaagttgtca ttaagctcac acctttcaaa
     2461 gtagtatata tatttatata aacataattt ttattcttaa acataattta aaatgcagaa
     2521 agtagtgtgt tatttgattt cgttctatga aattaactgt ctagtatcaa cgatcttatt
     2581 aacgctgata tcggatatgc ttaattttat agtaaat
//
LOCUS       DAKTIP010000259         2616 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.264, whole genome shotgun sequence.
ACCESSION   DAKTIP010000259 DAKTIP010000000
VERSION     DAKTIP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.264"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..171)
                     /locus_tag="O0305_002620"
     CDS             complement(<1..171)
                     /locus_tag="O0305_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275720.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            622..1662
                     /locus_tag="O0305_002621"
     CDS             622..1662
                     /locus_tag="O0305_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCY0275721.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYQPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1719..2561)
                     /locus_tag="O0305_002622"
     CDS             complement(1719..2561)
                     /locus_tag="O0305_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCY0275722.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
BASE COUNT          923 a          453 c          339 g          901 t
ORIGIN      
        1 gttattttta ctttcaatat cagaaataat ttctttagtt tttctttcaa tatcttcaaa
       61 atctttttta aagatataag caaatgctac tacaccaaca ccagcaacta atgttgtaat
      121 agcaactaag ctaaatacga atttaaacac acatttaata aaattccaca tctcaatttc
      181 acctctgtta aattaaatac ctatctacat ttcattatac tatcatatat agaataatca
      241 ttaaataaat acccactttc acgaaatata ttcatcaaat tatatagtga gcaagctaat
      301 gattattttc aaaaatgccg aggaacctct gtttcaacgt gacatgacct tatatgctaa
      361 tgttaacaat gactattatc gtgaatgcta acttaagatt gatatttatg ataagatgat
      421 tttaagtttt taactcaata tccccaccat ctataaaaat atgttttaac tgattgataa
      481 gtcttataca ccgagaaatc acctcaaaca attttatatt tcatttagta aaatataact
      541 ttatttaaca tgacattcaa tatacatacg attactattt atttacaata tattacaact
      601 aaaaagaaag gggccgatga tatgcaacaa gtgacatcag atatcatgac ttttagaggt
      661 tcacatttcg agttaggtgt taaaactggt aaatggcttc aacaaacacc tcttctaaaa
      721 aatcgagaaa aagaatggaa aaaacgtgtc ccacgtttcg atatagatgt aaatgaaact
      781 tatcaaatat tccaaacata cgcacctcaa atatgggaag aattaatggg actacaaagt
      841 atactcaaaa tgccaacacg ccaaattatt ttaaactttg gacattatcg ttttaccgat
      901 ttaaaagaaa gtggttgcac ggtctttcaa ggcaaagact tcatggttag aaattatgat
      961 taccaccctg caacttatga tggtcgctac ttactttatc aacctacaga tagcggttta
     1021 gctcaaattg gtcctgtatc aagggtaact ggaagaatgg atggtatgaa tgagtcagga
     1081 ttaactatgg gctataactt tatgcaccgg aagaaaccag caaatggttt tgtatgttat
     1141 atgattggtc gtctaattct tgaaaattgc cgaaatgtaa cagaagcaat ccaattattg
     1201 aaagaaatac cacacagaag ctcatttagc tacatactga tggacaaatc attgaaccat
     1261 gcaattgttg aagtgacacc gagatcaata gatgttcgtt atgataatat atgtacaaat
     1321 cattttgaaa tattaacaca tgaaaatcgt aattatacaa aagaatctaa agaaagactg
     1381 gcgcgtacca tttcacaaac taatgataat ttagatatga ctacagcatt taagttattc
     1441 aataatcctc agtatgaaat ttacagtaaa ctattcaaaa gctggtctgg caccatccat
     1501 acatcaatgt atcaacctga aacattaact gcatatttta cattgggaga aaatgcgcca
     1561 ccagaaataa tagatttcaa atcatggtta gatggtcaag accttaatat cactcacttt
     1621 actggcaata tagataccga cttaactttt gccaacaaat aaataacacc gatattagag
     1681 aaattatgct tcctaatatc ggtgcttaca tcctttattt attcaacttt gatattacca
     1741 tcaatcgtat aaaattctaa aacattgtcg cttttcccaa ctttaccatt agtaaatgtt
     1801 ttatttttaa ctacgctatc acccgttccc ggatttagct ttagtatagt gtcttcaggt
     1861 ttactatcat acttaaaacg aatatctcct tgtttagtag aagcttttgc atctactttt
     1921 gatggcatgt ttttaaattc aatgtcacca tgatcgttta aaaatatgga ttgtttaatt
     1981 tgactcttgt cgatttgtag cgtaccaata acaactttaa tattgctatt tttaatgtta
     2041 cttttactaa tataagtaga actattattt gtctttgaat ctagtgcgtc aagatttgag
     2101 tttttaataa ctacttcacc gttaatgctt tgaatacttg tgttctctaa atcaacatca
     2161 ctgattctaa cacttcctgc tccagatgat agatttaaac gtttaatcat tttatcaggc
     2221 atttcaatcg ttaacgtttt cttattctca tgaaaaggat tcatatcaat tgcatatcct
     2281 cttgtcttag accttttatc actaatcttc aacgtcttat ctactatatt aattaatata
     2341 tcattgtcac catcatattt aactctaaat tgattccccc gttttatacg aagttctgta
     2401 ctatccaaat ttatagatac attgtcaaaa gcatcgtctt tgaatgtttt atcatattgt
     2461 ttagtaccat atttgttttt atcatagctg aaccaaataa tggttgctgc tgtaaaaaag
     2521 acaacaaaca ctaaaagccc aataaaaaag aatttcttca ttttctaacg cttcctttga
     2581 cagttttaat ataccaaagt aaatatttta agatta
//
LOCUS       DAKTIP010000260         2557 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.265, whole genome shotgun sequence.
ACCESSION   DAKTIP010000260 DAKTIP010000000
VERSION     DAKTIP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.265"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1601)
                     /gene="bshC"
                     /locus_tag="O0305_002623"
     CDS             complement(<1..1601)
                     /gene="bshC"
                     /locus_tag="O0305_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCY0275723.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHI"
     gene            1796..2236
                     /locus_tag="O0305_002624"
     CDS             1796..2236
                     /locus_tag="O0305_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCY0275724.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            complement(2345..>2557)
                     /locus_tag="O0305_002625"
     CDS             complement(2345..>2557)
                     /locus_tag="O0305_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HCY0275725.1"
                     /translation="EFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNF
                     RGFDHNPGIIPDYEINSWKQLNDIVR"
BASE COUNT          825 a          440 c          365 g          927 t
ORIGIN      
        1 ataatatgat caaaagtgta agaaagtggt ggataggtgg agggcttgaa cacatctgtc
       61 ccaaaatcat tcaaaatttg aagtggattc catattcttt cttgtaatcc tcccattgga
      121 tggagtgttt cttgaatttc tctaaattgc ttcatactga tgtcgttttc tctttcaatg
      181 tttaaaagat aacgttttaa caaataatca tactgttgta tatgaatttc attattttta
      241 ttcacaaggt taatattatt ttgattcccc gccacttcat ctaataagtc tttgtttaga
      301 cgacgctgtt gttcaatcat accttctacc ttttcaataa attgatgtga tgcttgttct
      361 ctaataaact tacttctttc tccttcaaca ccatcgacta acactttttc taatggaata
      421 ttgtatttcg aaagtaattt ttctatacgg tcatttaaat aagtaattct aagccttggc
      481 atcacgatag gcatttcaac atcaaatagt tcaaatacat cttttagttc agcccagtac
      541 ttaatttcac tcggtcctcc aacaaatgcc accgtgttaa ataaccattc ttccattaat
      601 ggtcttgtca ctacattatt agaaaataat tcaggttgat tttccgcaat atttataatt
      661 tcttccttta tatatgtctt atctgtttta tttaatttaa aatgcttacc atcatacgaa
      721 actaattgac gcatattttc atcatgtaag aataaatgaa catttgtatc tgtttgtatc
      781 atcgcattca agccttgatt ttgagtacgt tgttgtgttg ctctaaaagc atcatcaagc
      841 aactgatgtt ttttcaaaat ctttttaaac ataggcgctt ccatttttct taacggctca
      901 aactgcgcat ctataaatag aacgccatat gctttaaatg tttcatgcag tagtgcttta
      961 aacatatcag tccacgagtc atattggtca ataattctgt cacaaatctc cagtagacct
     1021 tgtgtatgaa cagtttcttt catatgaatg aacatcgttt ttaaagtttg tttcaactca
     1081 gccttatcag gataatatct agagacagtc gtctctggca tctccattgt atgatattta
     1141 accttatgca gcgacccatg attttcgtta taaacaaatg tatgattcac ttcatcgaaa
     1201 tcatgatctt ctcctgcaat ccaaaaaact ggtactactt gttgcttatg tgtatccgtt
     1261 aattccttag ataaagtaat gattgaaaat attttatgga atgtatacaa tggtcccccg
     1321 aaaagccctg cttgttgtcc accaatcaca acttttgaac cattagctaa atgttgtatg
     1381 tttaattctt gttcacttga aagctttaaa tcactcatat attcacgaat aacattcgct
     1441 aatgccgctt ctcttccatt attttcttta gacatccttt tttcaaaact agtttgttga
     1501 gctgcatcat attgaaataa tcctgttatt acagggtcac tgctctttat ttttggtata
     1561 aactgatctt tttcatttaa actaactact ttacagtcca tgttttttct ccttaagtac
     1621 gcgattacaa actatagtat aaagtctata ccggtgattg acaattttac ggcttgaaaa
     1681 tcaatttaat catggaaaat ttataatatt cattgtttta cattttcaaa tcaatgaaaa
     1741 acacaagtgg tttaatgtat aataatagta gtaaacaaat aaggggtaga taaatatgag
     1801 tgaaatcaaa cgtcttgaaa ttaattacaa aactgacgaa ttattcgaaa actttagagc
     1861 gtttggcaac aaagacttat acatggtcaa tgagttaaac ggtcaaatga ttgatgcaag
     1921 ttcagattca ccattttatg gcatatttgt cggagatcaa ttaggagcta gaatggcatt
     1981 actaaaaaaa ggtgatgtcg aagaaatcta cttcccagat tttgaagatt atatattatt
     2041 atggaagtta gaagtattac caaaatatca aaacagaggg tacgcttcag aattgattga
     2101 ttttgcaaag agtttcaata tgccaattaa agccattggc agaaatgatt ctaaggattt
     2161 ctttttacat catggattta cagatgtgga agctaaaaat atagagggac atgatgtctt
     2221 attgtggaaa ccataagata ataatattcg acactacgag catgaaaatg catcttttcg
     2281 tagtgtcttt tttacaatta ctttcttaag ctaatataag taaatcattt tcaaattatt
     2341 tgtcttaacg tacaatatca tttagttgtt tccatgaatt aatttcataa tcaggtataa
     2401 ttcctggatt atgatcaaat cctctaaaat taaaccagca agtagctata cccgcattga
     2461 ttccacctag aatgtcagat gttaaagaat ctccaactat aatcgagtgc tgtctttcat
     2521 cctcaccaat atcattaaaa acataattaa aaaattc
//
LOCUS       DAKTIP010000261         2546 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.266, whole genome shotgun sequence.
ACCESSION   DAKTIP010000261 DAKTIP010000000
VERSION     DAKTIP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.266"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..540
                     /gene="vraF"
                     /locus_tag="O0305_002626"
     CDS             <1..540
                     /gene="vraF"
                     /locus_tag="O0305_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCY0275726.1"
                     /translation="SNKELSDIRKHDIGFIFQEYNLLHTLTVKENIMLPLTVQKLDKE
                     HMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFITLPSIIFADEPTGALDS
                     KSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKDGQIFTELYQGDDDKHT
                     FFKEIIRVQSVLGGVNYDL"
     gene            530..2419
                     /gene="vraG"
                     /locus_tag="O0305_002627"
     CDS             530..2419
                     /gene="vraG"
                     /locus_tag="O0305_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCY0275727.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLSKFNIIY
                     ILMLEQLLIFIITAILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLMLI
                     GVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYYL
                     SLNIVQYYDSIGTLMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSSS
                     IMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKANQ
                     LTSELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLND
                     KDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGITFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFKIM
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSKRT
                     IRHSI"
BASE COUNT          925 a          333 c          419 g          869 t
ORIGIN      
        1 tcaaacaagg aattatctga tatacgcaag catgatattg gttttatttt tcaagagtat
       61 aatttactgc atacattgac tgttaaagaa aacataatgt taccactaac ggtacagaag
      121 ttagataaag aacatatgtt aaatcgttat gaaaaagtag cagaagcatt aaatatattg
      181 gatattagtg ataaatatcc ctctgaattg tctggtggac aaaggcaacg aacatcagct
      241 gccagagcat ttataacatt gccttctatt atatttgctg acgaaccaac aggtgcattg
      301 gattctaaaa gtactcaaga tttattaaaa cgattaacaa gaatgaatga agcatttaag
      361 tctacaatta ttatggtaac gcatgatcct gttgcagcaa gctatgcaaa tcgagtagtg
      421 atgctaaaag atggtcaaat tttcactgaa ttataccaag gggatgacga taaacatacc
      481 tttttcaaag aaataatacg tgtacaaagt gttttaggtg gcgttaatta tgacctttaa
      541 cgagataata tttaaaaatt tccgtcaaaa tttatcacat tatgccatct atcttttttc
      601 gttaattacg agtgtagtat tgtattttag ctttgtagca ttaaaatacg ctcataaact
      661 aaacatgaca gagtcatatc caattataaa ggaaggctca caagtcggaa gctactttct
      721 atttttcatc ataattgcat ttttgttata tgccaatgtg ttatttatta aacgacgaag
      781 ttatgagctt gcattatatc aaacattagg tttatctaaa ttcaacatta tttatatact
      841 aatgctcgaa caattactaa tatttataat tacggcaata ttaggtatta ttattggtat
      901 ttttggttcg aaactgttat taatgattgt ctttacatta ttaggaatta aagaaaaggt
      961 tccaattatt tttagtttga gggcggtatt tgaaacatta atgttaatcg gtgtcgctta
     1021 ttttttaaca tctgctcaaa attttatatt agtgttcaaa caatctattt cacagatgtc
     1081 aaagaataac caggttaaag aaacaaatca taataaaatt acatttgaag aggttgtttt
     1141 aggcatctta ggtatagtat tgattaccac aggatactat ctatctttga acattgttca
     1201 atattatgat tctatcggta cacttatgtt tattttattg tcaactgtga ttggggcata
     1261 cttatttttt aaaagctctg tttctctagt ttttaaaatg gtgaagaagt ttagaaaagg
     1321 tgttataagt gtaaatgatg tcatgttctc atcatctatt atgtatcgta ttaagaaaaa
     1381 tgctttttca cttacggtca tggcaatcat ttcagcgatt actgtttcag ttctttgctt
     1441 tgctgctata agtagagcgt ccttatcaag tgaaataaaa tatactgcac cacacgacgt
     1501 tacaattaaa gaccaacaaa aagctaatca attaacaagt gaattaaaca atcaaaaaat
     1561 tcctcatttt tataattata aagaagtaat tcatacgaaa ttgtataaag ataatttatt
     1621 tgatgtaaaa gcgaaagaac catacaatgt aacaattact agtgataaat acatccctaa
     1681 tactgatttg aaacgtgggc aagctgattt atttgtagcg gaaggttcta tcaaagattt
     1741 agtgaaacat aagaagcatg gtaaggcaat tataggaacg aaaaaacatc atgttaatat
     1801 taagttacgt aaagatatta ataaaatcta ttttatgaca gatgttgatt taggtggacc
     1861 aacgtttgtc ttaaatgaca aagactatca agaaataaga aagtatacaa aggcaaagca
     1921 tatcgtctct caatttggat tcgatttgaa acataaaaaa gatgctttag cattagaaaa
     1981 agcgaaaaat aaagttgata aatctattga aacaagaagt gaagcgataa gctcaatatc
     2041 aagtttaacc ggaatattat tatttgtaac atcattttta ggtattacat tcttgattgc
     2101 tgtatgttgc attatataca taaagcaaat agatgaaacc gaagatgagt tagagaatta
     2161 tagtattttg agaaagcttg gatttacaca aaaagatatg gcaaggggac taaagtttaa
     2221 aattatgttt aattttgggt tacctttagt tattgcacta tcacatgcat attttacatc
     2281 attagcatat atgaaattaa tgggtacaac gaatcaaata ccggttttca tagtaatggg
     2341 attatacatt tgtatgtatg ctgtttttgc agtgacggct tataatcatt ccaagcgaac
     2401 aattagacat tccatataaa atatacagat ggctttcagt agagtagtgg attcggattc
     2461 acgaactata ctggaagctt tttattataa atgaagagaa gttatatttt tagcatgtat
     2521 agttgaatac tgggttaaaa taccat
//
LOCUS       DAKTIP010000262         2522 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.267, whole genome shotgun sequence.
ACCESSION   DAKTIP010000262 DAKTIP010000000
VERSION     DAKTIP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.267"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(245..1453)
                     /locus_tag="O0305_002628"
     CDS             complement(245..1453)
                     /locus_tag="O0305_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0275728.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1446..1613)
                     /locus_tag="O0305_002629"
     CDS             complement(1446..1613)
                     /locus_tag="O0305_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275729.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            complement(1588..2439)
                     /locus_tag="O0305_002630"
     CDS             complement(1588..2439)
                     /locus_tag="O0305_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCY0275730.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          914 a          416 c          317 g          875 t
ORIGIN      
        1 acagccgatt atgagtgctt cttaatatat taaaaatacc acttattttt attttcgaca
       61 taataatgaa gtactaatca agtggcaaat actcgtttca ttatgcatgt gctttataaa
      121 cggctaaact ttcgtaaaaa tttataagct aaacatgtgc tgataatcat aaatgtaact
      181 gcccaacatt ataagcacca taaaaaatac caacaaacat atagtcattg gtatttttta
      241 ttacttattc aactatttta ttactttctt taatatcata ttcacctaca tcattaccat
      301 agaaacgatt gtatcgacgt ttaaataatt ggaataaatg agtaactaat acttttaaaa
      361 tagcatatcc cggaatacct aaaataacgc ctacaatacc aagcaatttg cctgcacaca
      421 gtaaaatgaa aatgattgta agtggatgaa tcttaagtgt tttacccatg atatttggtg
      481 aaatgaagtg cccttcaaca aattgtacta aagtccatac tactgctaat tttaagagca
      541 tccacggcga tgttatagca gctattacaa tagctggaga aatcgctata gtaggcccta
      601 aatatggtac aacacttgta actgccgcaa tactagctaa tactaagcta tatttcaacc
      661 cgataaccga ataaccgata aacaacagta taccgataca gaatgaaacg ataatttgtc
      721 cttgaatgta tgaaccaact tgaacactca ttttttcaag tagatcatga aaatcttttc
      781 ggaatttcgg tggcataata ttcgttgaaa attctttgaa gtgatgtcca tctttaagca
      841 taaagaatag tacaaatggt gttgtgacaa tcacaacgcc aatattagca atcgtttctg
      901 caaacgttgc aattttagac ccaaagccat ctgtaaattc acttaacata gatggtattt
      961 tcttaggtaa agaatttaac caatcattaa tttgactata atacgaagag aaaaacgtat
     1021 tatttgctat tttatcaata gaattaatta atttttctag atattgcggt gaatttttaa
     1081 ctaaactatc tacttgcgaa ccaataatag gtatcaataa attaacaatt aacgtaataa
     1141 cacctactac agctagataa ataatagaaa tacctgcaac tcttggtatt ctataacgtt
     1201 ccatcatatt tacgattggg ttaaatagat agaatagaat taaagagacg atgataggtg
     1261 ctgctatcgt cttaaaaacg atgataaaag gatcaaaaac atatgatact ttttcgaaaa
     1321 taaaaataac aatacccaac aatactagcg ctattaatga gaagactaaa tcattcccac
     1381 caacaaactt catataccgt gattcggtaa attttaaaaa attctttctt atattctttt
     1441 cattttcatt caattataga ttcaccacca cttaaaatat tcataagatg ttgtataaaa
     1501 acagtccact tatgtaaaat tactaacatc aatgatacca tacaaatacc ctttacagta
     1561 acgaaacgcc cgtgtaatac acttaattta ggattgatta atcctttctt caaaatgatg
     1621 aatgtgtaat gttaaatata taatttcaga ctcactgata ttaacatcaa attgtttttg
     1681 tatcatagtt aaaattttat atgctgtgtt atagcaaatc ggatagtgat ttttaatcat
     1741 agacacaaaa tcatcttgtg catgtatata ttcttttctt cttaatcggc gaattaaaaa
     1801 ttgtacgtgc cttataaaac gttggtattg taatgattcc ttatcaacag ttgtcacaag
     1861 gtctgattca atgatatcta tacctttttt aataacatta ttgatcaagg tcatctcatg
     1921 catagataaa tcttctgtat tagatgcaat atgtaatgca ataaatccta tctcatcttc
     1981 aggaaaatgt acatctaatg ctgcatttaa ctgatcaatc acctgtttag caatatgata
     2041 tgcatcacta tataactgca tagtttccat aacaaatgga ttgctaataa cttgattttg
     2101 ttttaagcgt ttataagcaa atataatatg atccgttaat gaaactacaa gttgttttga
     2161 atcaacattc atcgcagtat tagaaataaa attcaacgaa tcaataatta cttgtaatac
     2221 attatcatca gcgatttcaa ctaaactttt ataatgtgct ttttgttgct cactttctaa
     2281 tttataaatt ttctctattg taatagtttg gtcgtttaac gccattccct cttttttgtt
     2341 aaaaccaata cctttaccga ttaaaataac ttcttgatca ttattagtac atactacgac
     2401 attgttgttc aatgttttag taacaatata ttctcccatt atcatcacct atttttctat
     2461 tattaagatt atatatcgga aatgtctaac ttgaaagtat aataatttaa atacttgtgt
     2521 tc
//
LOCUS       DAKTIP010000263         2504 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.268, whole genome shotgun sequence.
ACCESSION   DAKTIP010000263 DAKTIP010000000
VERSION     DAKTIP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.268"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            323..424
                     /locus_tag="O0305_002631"
     CDS             323..424
                     /locus_tag="O0305_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275731.1"
                     /translation="MKFAANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            434..706
                     /locus_tag="O0305_002632"
     CDS             434..706
                     /locus_tag="O0305_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCY0275732.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNWWVNRAYYNIY"
     gene            750..>2504
                     /locus_tag="O0305_002633"
     CDS             750..>2504
                     /locus_tag="O0305_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCY0275733.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRIDQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKAATITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYYKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKEN
                     KLSIQFINKNDCRIFSFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKL"
BASE COUNT          976 a          311 c          384 g          833 t
ORIGIN      
        1 aaggaaaatc atactgtggt agaaatcaat tatgattata tggtagtact tataaataag
       61 tgatttctat tgaatatgta atgaagttca tatattaata taactttcat tgatatagaa
      121 gtcgcaatga ttaatttaaa caataaaaaa caaccatcat ttccaaactt tgacaacgaa
      181 tgatggttaa agaaaatctc atttagtcac cgtcttttta acgggctcaa taggcgacat
      241 gttcctgcat atcgtctatt tatacactgc acaatttaat aataatacaa ttatactttg
      301 atgtccattt aacataaagt ggttgaaatt tgcagcaaat aaattaacct tgactatttt
      361 cagaaaattc tgtataaata acgaaaaaga gatatggatt aaatatgaag aggtgttttt
      421 atagaagaat aaaatttttg gcactatatt atatttaact ttagcacttg gattatcaac
      481 agcagcttat gcatctacag aatacgcaga aggaggcact tggagtcatg gtgtcggcag
      541 taagtatgtt tggtcgtatt attatcatgg tcataaagga catggtgcaa cagctattgg
      601 aaaatataga tcatttagtg gttatacaag agctggtgta aaagcaaaag catcagctac
      661 taaacataat tggtgggtca atagagcgta ttataacatt tattaaatac taaaaaatca
      721 tagaaaataa acaacgcaag aggtacaaca tgaaatggtt caaattaata cttgatgtta
      781 caatcttcat tctgattgct atattgttat ttgtttatac atataaagaa aacgaagaaa
      841 tattgcctga tactaaatac cctatagcgg taactgactg gaataaaaaa tatagtaaaa
      901 atgagattta taaacgtata gatcaattcg ctaaaaatga gaacgtagca atctataaat
      961 caacttcaaa ttatacaaac aaaaacgtag ataaagatat atatgtattt aataaatcaa
     1021 aagcagcaac tatcactcct tttaatgcta aatataacat tcattattta agtgacgacg
     1081 aagtattaaa aaaagatatc aaagggagtt attttgtaaa agacaaaaat tttgacgtgt
     1141 ctaaattcat aaatttttta aaagaatatg gtgttactgc tgaatcatac aaaatagatc
     1201 atatgatgat tgccgttggt gtcattaaac aaatgaatat agtagttcca ttatcctcac
     1261 ttttaatcgt ttattttatt tattatattt tcgaaaagaa cattaatttc aaagcgtatg
     1321 cgattaagta tttaaatggt tttacattaa gaaaaataat tttcgaaaat ttttcaaaga
     1381 aatgcacgta ttgggtaacg ttaatcataa ctcaaattct tttaactaca agtgtacttt
     1441 ggatattaaa ttacacgggt aatttagatt tattcatatt aagaatagtt ctgctttcat
     1501 gtcttttcat tttaacgatt agtgttatta atttatggac tttcttaatg ttactaaatt
     1561 taaatattgc aaatatgatt aaaggtaagc agcactttaa aacaattcgt tttattaata
     1621 cagtttgtaa aagtattctc ttagtactaa tagctaatgt aatgatagaa aatactagtg
     1681 ttattaaaga tttgaataaa ataaaagaaa ctgagaaata ttggaatgta ttagatgatt
     1741 attacacgat tgaatttgca ccttatcacg aaacaaaaca aagtttgatt gataatatgg
     1801 tgcgatcaga acaattagta aaggctagtg aagcagaaaa taatgcgatt ttattcaaac
     1861 caaagggtga ctccgttgac aatgacaact tttcgcctga tgaggggaat gtaatattag
     1921 taaataatca gttctggtcg atttattaca agcagtttca acctgatatt ccgataaaaa
     1981 atcaaaaaaa taatgtcgaa gtaattattc cacaaaagtt tcatgcaatg cgtaatgaaa
     2041 tcaatcaagc atatcattca tggtttgaat ttgtacaaaa taaaaataat aaagagaata
     2101 agttatctat acagtttatc aacaaaaatg attgtcgaat tttttcattt gatgcacgag
     2161 atagtcgcca tttgtcattt atagaggcgc caatcattgt gaatgttcag gcatcagatt
     2221 tatcgaatga tttttattat gccatgatca gtcaaggcgg gtatttattc aaaaattatg
     2281 acgcgctagt aaaaaatatt gaaaagtatc atcttgatgg ggaaatcagt ggaataacca
     2341 attataaaga tagcgtgatg gaaatgtatc atgaaaacaa tttgaaatta acagtactca
     2401 acttttcaca aatcattatc gcaatcattt taataattat tattttattt gatgtgaaat
     2461 attattttga acagcatcga aaattactcg taatcaaaaa gcta
//
LOCUS       DAKTIP010000264         2493 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.269, whole genome shotgun sequence.
ACCESSION   DAKTIP010000264 DAKTIP010000000
VERSION     DAKTIP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.269"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(88..537)
                     /locus_tag="O0305_002634"
     CDS             complement(88..537)
                     /locus_tag="O0305_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCY0275734.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            complement(904..2433)
                     /gene="aur"
                     /locus_tag="O0305_002635"
     CDS             complement(904..2433)
                     /gene="aur"
                     /locus_tag="O0305_002635"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCY0275735.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
BASE COUNT          700 a          468 c          344 g          981 t
ORIGIN      
        1 aaaaaacgcg tacacaagtt aatatttggt aactaacttg agtacacgtg atacgaaaca
       61 aatttttatt attcatttga tacatgtcta tttaaataat tttgcatata cgtattctgc
      121 catatcattt gaggcagtta agattaatgt agtgtcattt ccactcaccg tacctaatat
      181 ctcttctata tttagctgat cgataaaata gtttatgcct tgggcgaaac caggtgaggt
      241 tttgacgata atgtatgaac cattgatgat tgaacttaga acttcatgtt cacaatagtg
      301 tctaaacttt tctctcatct cttgttctgt ttgattatta atagctttat aaatccatgt
      361 ctcacaatcg atagggacac gatatatatt tagttccttc aagtctttag caattgttgt
      421 tgcactatat cttacaccaa aatattcttc aatatatgaa atgatttgtt cttttttata
      481 aatcttatgc tttttaacta ttgtagaaac aatttctaat cgtttacttt tcttcatatt
      541 gtaaactcct ttggtagtta cgtttcttgt attaaaaaat aaattcatgc atgtttcatt
      601 tataatttaa cactttgttt tgcaaaatat aataaaaata catgtaaatt ttttgtgaca
      661 acttttaaaa tgaattttgt attctaagtc agcatttaat tatcacatat ctactacttg
      721 taatgatttt agactgccga gtagtctttc ggcagacaac cctcacactc ctctcatcta
      781 attacaaaga gagggtatac ctacaaagtc aattatcaat gtaggtatac cccatatata
      841 agctgtattt aaaatttaat tatttataag tgtttaacat tacttcttct tgtttatata
      901 tttttactcc acgcctactt cattccatgc ttcatacacc tgttcagctg tttgctcgtc
      961 atataaatct ttagccgctt ggtataatgc atctttacaa tctttgaagt ttgaattact
     1021 tgttaagtat tccgttaatg ctcggtagta aatttgttct gatttagatt tccctattgc
     1081 ttgaatcacg ttataagctg ctttatttgg aattccagaa ttcgtatgta cgccaccatt
     1141 atctttttca gtgaatacat agtctttcat atgagctggt tgaccaaatt gttctgggtt
     1201 tgacatgctg cgtaaagcgt ctccctcttt tccaggtgtg tagacatctt cacccattaa
     1261 gaaatcctcg tcatctacaa agtatccaaa aacatctgaa aagctttcat ttagagcgcc
     1321 tgactggtcc ttatattcta agttcgctgt ctcttgtgtc acaccgtgtg ttaattcgtg
     1381 tgctactacg tcatttgcac ccgataaact tgtgaatgtg cgaccatcac catcaccata
     1441 gatcattttg tcaccgatcc atgcggcatt atttctgtta tcttgaccac cgtagttatt
     1501 aacatgcgtt aatgaaacaa ttggactacc ttggttgtca tatgattcac gaccaaatgt
     1561 gtctttgtaa taatcatatg tttgtttagc gtaataattt gcatctacgc cagcacgttg
     1621 ctcatctttt acgaagtttt catcttcatt agtaatcaat gttgcttgac ctgtttgatc
     1681 attaaagcta aatgctgata atttaccttg atgcgttaaa tcttctaggc taaatccacc
     1741 gtcaatacta ttgatattga tatcttttgt atcgccaagt acaccttttc ctttaccagt
     1801 ttctgcagct tctttaacta agttcatttt ttctaaaatt tcgccagttt gagcatcaat
     1861 tttaactttc caatgtgaaa tttctggtgt cactgtaatt aactcaacat tataaacgta
     1921 tttattactg tcaccatcga tttcaacttt gttttcttta atgactttat ctttaagatt
     1981 tttcgcttta ttcttatcaa tcttaactgc tttaaatgct ttgtcggctg cgtcatcttt
     2041 acttaatgtc actttattcg ttggctttac tttcttcgca tcagtatccc cattgattaa
     2101 aacgactttt cctgatttgt ctgcgtgtac tttcacttct ttgtcaggtg catgaacacc
     2161 atcaacactc ggttgcaatg tataatgcgt aaatcctttt ttatcagttt ttacatcagt
     2221 aacagcgtaa tcttgataaa tattttttac attttcggat ttaggcaatt cttttaatgc
     2281 tttgaccgca tcactctttt gagtcacctc aaatttaata tcagaattag ctggtttatt
     2341 ttttgaatca atcgctaatg ctgctggtga taaagtgctc aacaaggtta atgctgccat
     2401 acttgtaaat gcatatcttg aaaatttcct cacatttcat tcctcctgaa atcttaaaaa
     2461 cagcttataa ataaaatatt aatttaattt ttc
//
LOCUS       DAKTIP010000265         2461 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.270, whole genome shotgun sequence.
ACCESSION   DAKTIP010000265 DAKTIP010000000
VERSION     DAKTIP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.270"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            47..730
                     /locus_tag="O0305_002636"
     CDS             47..730
                     /locus_tag="O0305_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCY0275736.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            748..1737
                     /gene="floA"
                     /locus_tag="O0305_002637"
     CDS             748..1737
                     /gene="floA"
                     /locus_tag="O0305_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCY0275737.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            1754..2452
                     /locus_tag="O0305_002638"
     CDS             1754..2452
                     /locus_tag="O0305_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCY0275738.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQQETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
BASE COUNT          875 a          366 c          484 g          736 t
ORIGIN      
        1 aatgtgaggg cgaggtgaaa ttgtgagtta taataatttt ttacaaatga caactatctt
       61 ggaatcaacg gctggagata cttgggttga acaagttagc aatataattg ttcaacctat
      121 ttttacgtta atattaacct gtttgacatt cttaggattt gtatatcaac tttactctaa
      181 aaaaatcaat gcagctggta ttatcgccac attatcatta cttattttat ttttgggatt
      241 tctaatccaa ggaaatgtca atatgcattc tatcttaata ttctcaattg gcgttatatt
      301 agttgtaatt gaattatttg tagttggtgc agtaattggt attattggca tgatactgat
      361 aactataagc ataacaacgc tcggtgataa tttgctattt atgcttgcga atgttatcgt
      421 tgccttgatt ttaacgattg tagaatgggt gatattagtg aagattttca acagaaagat
      481 tccgtttttg gataaagtta tcttaaaaga ttcaactaat tctgagtcag gttacaattc
      541 tcatgataac cgctcgcacc tcgtaggaaa gactgctcaa acagttacag atcttcgacc
      601 tgcagggatt attttttgtg aaaatgaacg tattgatgct gtttcagatg gcaactttat
      661 tttgcgcaat aaaacggtaa aaatccttga agttgaagga acaagagtag ttgtgaggga
      721 agtagattaa ttaaaaggag cgataccatg tttagtttaa gttttatcgt aatagcagtt
      781 attatagtag ttgcattact tattttattc tcatttgtac ccattggttt atggatttca
      841 gcgttagcag ctggcgttca tgttggtata ggtacattgg ttggtatgcg tttacgtcgt
      901 gtatctccaa gaaaagttat agcgccatta attaaagcgc acaaagcagg actagcatta
      961 acaacaaacc aattagaatc gcattatcta gcaggaggaa atgttgacag agttgttgac
     1021 gctaatattg ctgcacaacg tgctgacatt gatcttcctt tcgaacgtgc tgctgcaatt
     1081 gaccttgcag gacgtgacgt attagaagcg gttcaaatgt ctgttaatcc taaagtcatt
     1141 gaaacaccat ttatcgcagg tgtagcaatg aacggtattg aagtgaaagc caaagctcgt
     1201 atcacagtta gagctaatat tgctcgactt gttggtggtg ctggtgaaga aacaatcatc
     1261 gcacgtgttg gtgaaggtat cgtttcaaca attggttcta gtaagcatca tacagaagta
     1321 cttgaaaacc cagataatat ttctaaaaca gttttaagca aaggtttaga ttcaggtact
     1381 gcatttgaaa ttttatcaat tgatattgct gacgttgata ttagtaaaaa tattggtgca
     1441 gacttacaaa ctgaacaagc attagcagac aaaaatattg cacaagcaaa agctgaagaa
     1501 cgtagagcta tggctgtagc aactgagcaa gaaatgaaag cgcgtgtaca agaaatgcat
     1561 gctaaagtag ttgaagccga atctgaagta ccattagcta tggctgaagc attacgttca
     1621 ggtaatatca gtgttaaaga ttattataat ttgaaaaata tcgaagctga tacaggcatg
     1681 agaaatgcaa ttaataaacg aactgatcaa agtgatgatg agtcacctga acattaagtc
     1741 gagaggtgat taaatgagtg tcggtattct aatttttgtc atatcagtga tcatttctat
     1801 cattactact atgcgcgaaa atagtcataa agatagacaa aatcaaaagc cacctcaaaa
     1861 aacatctacc gataatgaac caaaaaaagg tggctttttt gaagaaattg agcgaacgtt
     1921 taaagaaata agtgaagaat taaatgaaga agaaaagaaa tcatcgaaac gaaaatatga
     1981 tgatacgtta ccacctttat tcgatgaact tccaaaggaa gagcctaaat cgaaacctgt
     2041 tgtagaacct atggcaccta aaaaacaaca agaaacaaaa ccgatgacag agaaaccaat
     2101 cacagtgcct aaagcagaac cggtggagca gaaacataga ccttctagac aagataattc
     2161 tgacgaaatt agacgtcaat tagaaaaatc acttagagat gatattaaaa cgattcgtac
     2221 tgacattgat agagaaaaag aaaagcaaat tgctaaaatg gaaaaacgtg ctagagatat
     2281 tattgaggat aaatacttat ctgaacgtac aaaacgtttg aaattaaagc agctgcttaa
     2341 ttctcaaaat gtcgaaaaag atttgactaa atcagcgttc caatttgata aagatgaagt
     2401 aatcaatggt atgatatggt cagaaatttt agctaaacca aaacaattat aaaatttttt
     2461 g
//
LOCUS       DAKTIP010000266         2461 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.271, whole genome shotgun sequence.
ACCESSION   DAKTIP010000266 DAKTIP010000000
VERSION     DAKTIP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.271"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..164
                     /locus_tag="O0305_002639"
     CDS             <1..164
                     /locus_tag="O0305_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCY0275739.1"
                     /translation="LYNGEFNKGQIKITADGNNYTIDLSKKLKLTDTNRYVKNPRNAE
                     IEVILEKSN"
     gene            259..984
                     /locus_tag="O0305_002640"
     CDS             259..984
                     /locus_tag="O0305_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCY0275740.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1092..1808
                     /locus_tag="O0305_002641"
     CDS             1092..1808
                     /locus_tag="O0305_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCY0275741.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
     gene            complement(2253..2450)
                     /locus_tag="O0305_002642"
     CDS             complement(2253..2450)
                     /locus_tag="O0305_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275742.1"
                     /translation="MTELNNIINSLQSLFESESGYKISKNSGVPYQTVQDLRNGKTKL
                     EDARFRTIIKLYSYYVSLKEH"
BASE COUNT          921 a          377 c          356 g          807 t
ORIGIN      
        1 agttatataa cggagaattt aataaaggtc aaattaagat aactgctgat ggaaataatt
       61 acacgattga tttaagtaaa aagttaaaat taactgacac aaaccgttat gttaaaaatc
      121 ctcgtaatgc agaaattgaa gtcatactcg aaaaatctaa ctaacctatt accttttgta
      181 aatgcggata atttcaatta tctaattaac ccctttttat aattaaacat tccaacaata
      241 ctcaaaggag aaattcgaat gaacaataac atcacgaaaa aaattatttt atcaacaaca
      301 ttgttactat taggtacagc atctacacaa tttcctaata cacctatcaa ttcttcatct
      361 gaagcgaaag cttattatat aaatcaaaac gaaactaacg ttaatgagtt aactaaatat
      421 tactcgcaaa aatatttaac cttctctaac agtacgttat ggcaaaaaga taacggtacg
      481 attcatgcaa cgttgttaca gttttcttgg tatagtcata ttcaagttta tggacctgaa
      541 agttggggca atatcaacca attaagaaat aaaagcgttg atatttttgg cataaaagac
      601 caagaaacca ttgattcttt tgcattatct caagaaacgt ttactggtgg tgttactcct
      661 gcagcaacat ctaacgataa acactataaa ctgaatgtaa catataaaga taaagcagaa
      721 acgtttactg gcggatttcc agtttatgaa ggcaataagc ctgttttaac tttaaaagaa
      781 ttagattttc gtattcgtca aacattaatt aaaagtaaaa aattatataa taattcttat
      841 aataaaggac aaattaaaat aacaggtgca gacaataact acacaataga tttaagtaaa
      901 aggttgccat caactgatgc aaatagatat gttaaaaaac ctcaaaatgc aaaaattgaa
      961 gttatcctcg aaaaatcaaa ctaacaataa taatggagtt aataaaaata atcgcaaata
     1021 ctatattgac ttcgctcaca tttaaatttc ttattcctcg tatcatgatt cctctgaaag
     1081 gagatgttct aatgagtaag aacatcacga aaaatataat tttaacgaca acattattac
     1141 tattaggtac tgtattacct caaaatcaaa aaccagtatt tagtttttac tctgaagcta
     1201 aagcttatag cattggtcaa gatgaaacta acatcaatga attaattaaa tattacacac
     1261 agcctcattt ttcattttca aataaatggc tatatcaata tgataatgga aacatttatg
     1321 ttgaacttaa gagatattca tggtcagcac atatatcttt atggggcgct gaaagttggg
     1381 gaaatattaa tcagttaaaa gatcgttacg tagatgtgtt tggactaaaa gacaaagata
     1441 ctgatcagtt atggtggtct tatagagaga catttacagg tggcgttaca ccagccgcaa
     1501 aaccttctga taaaacttat aatctttttg tgcaatacaa agataaacta caaacgatta
     1561 ttggtgcgca taaaatatac caaggcaata aaccagtatt aacattgaaa gaaatcgatt
     1621 tccgtgcacg agaagcgtta ataaaaaata aaatattata taacgaaaat cgtaataaag
     1681 gtaagcttaa gatcaccggt ggcggtaata actacactat tgatttaagc aaaagattac
     1741 attcagatct agcaaatgtt tatgttaaaa atcctaataa aataactgtt gacgtcctct
     1801 ttgattagta tatgaaggtg acttatactt catgcacttt aattccaaat cagattattt
     1861 aaatgataat ttttaaagtg tatgatgtat ataataggta aaattttcta tatatttaaa
     1921 tggaattggg agtaggaatg tgacagaaat agtattttat aaaatttatt tcgttgtcac
     1981 tccccaactt gcacattatt gtaagctgac ttttcgtcag cttctgtgtt ggggcccacc
     2041 ccaacttgca ttgtctgtag aatttctttt tgaaattctc tatgttgggg ccccgcctat
     2101 aattgaaaaa tgcttgttac atgggcattt tcattcggtc aactactacc aatataatat
     2161 tgtagagcct aagacattga tttattatgt cttaggctct attccttcat ttaatgatta
     2221 aattattata gcaatacttt attgtcccat gattagtgtt cttttaatga gacatagtaa
     2281 ctataaagtt taataatcgt tctaaatcta gcatcttcta gtttggtttt cccatttctt
     2341 aaatcttgta ctgtttgata tggaactcct gaatttttcg aaattttata gcctgattca
     2401 gactcaaaca aagattgaag agagtttata atattattca actctgtcat ttttaatccc
     2461 c
//
LOCUS       DAKTIP010000267         2440 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.272, whole genome shotgun sequence.
ACCESSION   DAKTIP010000267 DAKTIP010000000
VERSION     DAKTIP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.272"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..348)
                     /locus_tag="O0305_002643"
     CDS             complement(<1..348)
                     /locus_tag="O0305_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275743.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVL"
     gene            complement(362..1153)
                     /locus_tag="O0305_002644"
     CDS             complement(362..1153)
                     /locus_tag="O0305_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCY0275744.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQGDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            complement(1334..1537)
                     /locus_tag="O0305_002645"
     CDS             complement(1334..1537)
                     /locus_tag="O0305_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275745.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(1566..2375)
                     /locus_tag="O0305_002646"
     CDS             complement(1566..2375)
                     /locus_tag="O0305_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0275746.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          749 a          447 c          328 g          916 t
ORIGIN      
        1 taatacgcgc atatcttcta ataatttaaa aagttgttga taaaatttag ggtgtttaaa
       61 agttttatct tgtactactt catttacaat ttgtacatcc atcatatgat aaccgtaagc
      121 agctaacata acatctgttt ttaaaccagc catttcgata tggcttggtc gatgtgacca
      181 aaaccaactc gtaatcaaaa cattttgaac aggattatag taagggaatt tttggatttt
      241 aacttgtgtt tgttcatttt ttaataagag tcgagctaag tcttgcaaca tcattacttg
      301 cttggcatct aactgttcgt cattaaattt tatgaaacga tcactcatgt tttatccctc
      361 tttaaaagtt tagttctaca gcattatata ttgcttgttg ttcacgttca tcttccaatt
      421 tatcaataat tgtacgttta attgcacgtt caactggcat tacagtgatt aaatcacaca
      481 agtctaataa tgcacggata ctagcggctt cttcagaaat ttgtccctgc ttagacatag
      541 tacgtaaatc ttcattaaac ttaataattt gttcgatttg tttatcacct tgtaataaac
      601 tttgctcttt aatcacattt tttaaaatgt ccccatcaat ataatcaacg tgaataacaa
      661 caaagcgatt ttttagtgct tcattcattg gcaaagtacc aacataacct tcatttatcg
      721 ctgctataac gttaaatcct ggtacagctt tgattacttc accagtgtat ggattcgtaa
      781 tttgacgacg ataatctaag accccattta atacaggcaa tgtttcaggt ttagccatat
      841 ttatttcatc aatatataaa atatgcccct ctttcatagc tttaataact ggaccatcta
      901 caaagacaat ttcttgttga ccttccgcat ttgttttaat tgttttaaag cctaataagc
      961 tttctgtatc taaatcaaca gaacaattga cttgatgcat gggtgtatca acaacttcac
     1021 ttaatgtttc tgccaacttt gttttccctg aacctgttgg acctttaagt aaaatatttt
     1081 tatttaaatc aaataatgcc ttcgcatcat tgaaaactgt tgaatctgaa ttcttataat
     1141 gttttagtgc cattttaaaa cgttctcctt taaataaatt aaaaaactaa gacatggaat
     1201 aagttctcca aatgccttag tttactattc tattctgata taaaacgcaa tgcttagtat
     1261 gcgaattgtt tatattttac caaatttact aattgaaaat ctcatattag gcttcatttc
     1321 aataattatg cgtttaaact tctttgaaat atgttttata gtaaccgcca actaatccag
     1381 aattatctat tatttgataa tattcttcag tttcgttgat aacatcatat tgtttgccaa
     1441 ccgttaacat gtcagaaact gtaaatttct ttgcatctgt attaatgact tctactttct
     1501 tgattggtgt acgttctttc caagtttcat gtaacataaa ttttgtcctc gctatcgtaa
     1561 aaaaattatt tttgtaaagt gaatataaac gataatccac tgttttcagt aattctaatt
     1621 gattcaatat catcttgctc aagcgtttga tatggtattt ccacttcatc taagcgcgat
     1681 ttcgcttgat taaagaaatc tgcatctttc gtttcaaact caacttgatc gactgcgcca
     1741 tattccggca ttgtcatttc ggtttctaca ggcattaaat gtatttcgcc acctagacca
     1801 cccgttccaa ctttgaatac ttggacatga tagtcagcat tgtcgaaggg ttggtattct
     1861 gcaaatactt caagtccgaa tatatttgtt aaaatttgac ctgtaatatc tacatgattc
     1921 actttaagaa tcactggtcc taaaccttgc acttgatgta acggattgac cgcactctca
     1981 aaagaaggca tacctaaacc aacgccataa ttattctcgt ttgaatatat tgagaaaata
     2041 tgaccattgt tatcttcgag actgaaatat ttattgccat ttaattcttt aactgttgta
     2101 aatggaatat ccttattcga taatatttcc gcatactcct ctaaacctga gtcagttggt
     2161 gtacgtaatc caatacccac aaagtgagat tcttctaatt gctcacttgg aacttgtata
     2221 aattgaagtc ttgttcctgg gcttatatct gcatcgccga aacgaattga gttttcaaga
     2281 agttcttcat aatttaatcc taatatgtca accatgaaat gttttgtctg ttctatattt
     2341 gttgtaccta atgttatact tctaagtcca cacattatag ccacctctat ctttatcttt
     2401 actattatat tataacttat tttaatcata aattatagaa
//
LOCUS       DAKTIP010000268         2416 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.273, whole genome shotgun sequence.
ACCESSION   DAKTIP010000268 DAKTIP010000000
VERSION     DAKTIP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.273"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            17..223
                     /locus_tag="O0305_002647"
     CDS             17..223
                     /locus_tag="O0305_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275747.1"
                     /translation="MNEIETIISEIEKLLTNNTPYSISKNSGVPRQTVTDLKVGKTKI
                     KEAKFKTIIKLYEYQRTLENKTEC"
     gene            924..1034
                     /locus_tag="O0305_002648"
     CDS             924..1034
                     /locus_tag="O0305_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275748.1"
                     /translation="MKMTIKVRKTNKKEKIEFLIGTFIIILVILGFKIMK"
     gene            1462..1647
                     /locus_tag="O0305_002649"
     CDS             1462..1647
                     /locus_tag="O0305_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275749.1"
                     /translation="MSEYKKKIIELIESNLTGYEISKKTGVSQYVLSQLRQGKREVDN
                     LTLNTTEKLYEYANKVL"
     gene            2159..2263
                     /locus_tag="O0305_002650"
     CDS             2159..2263
                     /locus_tag="O0305_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275750.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
BASE COUNT          995 a          238 c          375 g          808 t
ORIGIN      
        1 gagagtgtga ttaataatga atgagattga aactattata agtgaaatag aaaagttatt
       61 aactaacaat acaccatata gtatttcaaa aaactcaggt gtaccacgtc aaacagttac
      121 tgatttaaag gtaggtaaaa ctaaaataaa agaagctaaa tttaaaacga taatcaagtt
      181 atatgaatat caaagaacat tagaaaataa aacagaatgt taacaaaaat attttgttag
      241 gttaggtgga caagaaatta aatttctaat gatttcatgg ttgtctgtcc acgttttgtc
      301 cacctcaata agataatctc ttagttttag aaattgtaca tggttaaata atcaaaaatg
      361 catattatga aattatcatt gccattaaat cttgaatgat ctttaagcga tgatataaaa
      421 ggctgaacac agtaaataag ttaactttaa tgcagtattc tatactatac atattttgta
      481 ataatgttta tgcacataat ttttcattga caatttttta tattaaattg tgtaattggc
      541 atatatgatg atttgtgata attatattgt aatatagcat ttggttttat gattctaaaa
      601 ttaatagatt tgaaaagtat actttatttc atgaaagttt ttggtacatt tatttgaaaa
      661 tgtttttatt acaaagttac atggctaaat gtttcttaac aaaattaatt tttcgagtgt
      721 aaagaatcgt gtggtaaaca taatttatat agattataag gaggtgaaaa tggtcgagga
      781 agttaaaacg ataaaatagt ttgaatgttt tgatttcaat tctggtagga ctattgaatt
      841 tgcaatctaa taagaatgtg aggtttgatt taatatgaag tcatattctt tgaataaaat
      901 attactttct caccattagt aaaatgaaga tgactattaa agtgcgaaaa acaaataaaa
      961 aagagaaaat agaattttta atagggacgt ttataattat tttagtaata ttagggttca
     1021 aaattatgaa ataaatgtat agtatttgag aagtctatat gactccggaa aaattaaatt
     1081 ctaaagatat ctcaatatct caaatttaat agactactgg agttagtagt ttgaatattg
     1141 aaagacacta atctaataaa aatacataaa aggttaaact ttagaagtgg ctgtaaactt
     1201 caatgaatac taaacattga agttttgtaa aaggcgtaag gtatacaaga caaatcgttt
     1261 atctttgaca aagtaattga tgatgaaaag tcgttaatat gtagaaacaa aaattgaaaa
     1321 tataaaattt aactattatg aagtgatgat agccattaat gaatagaaaa ataataaaat
     1381 catttataga taaaaaatac gttaagttca cagaatttcg aaaaaaagca gaaaacattg
     1441 tacagatatg gagatgaaaa aatgtcagaa tacaagaaaa aaataattga attaattgaa
     1501 agtaatttaa caggatatga aatttctaaa aaaactggag tttctcaata cgtactttca
     1561 caattaagac agggcaaacg cgaagtagat aatctaaccc tgaatacaac agaaaaatta
     1621 tatgaatatg ccaataaagt tttgtaattt aactaatgta taaattaatc aagctatgtt
     1681 tatttgattt aactattaat aaaaatcata tggtgaatgg atattataat aattaaaata
     1741 caaaaatagt agattccaat ttgtgaaaaa ggagtatgtc gtttcattta gaatttattg
     1801 cacgaaatta gcatcgaata agggaaaata agtgttaagt tttaaatgat aaaaaagatt
     1861 ggaatggatc gtcttgaaat gctcccttca aagttttcat tttttcaatg tcgacttcga
     1921 agggggcatt ttcattaaat tgttatagct ttttatattt gtataatgaa catataagtt
     1981 taagaaggtg cgagtgaagg aaataaaaaa gctcaaatgt accaaattgt taatcttaat
     2041 aaatctctac tttataaaga ttgaatggac attcgagcgt taatcagtca ggagggactt
     2101 tccctcctac aatttaataa taatacttgc ttcaccacta tacaaggagt gagttgttat
     2161 gttcaaagtg aattattcga ttttaagtta ttatccagaa tataatatcg cagtaagttg
     2221 gcaacgttta agagaaggaa aaacaataaa aaacaagatt taatactgct gcgtcatgag
     2281 gcgcttgaac attatttgat gaataagtat aatttcaact atgattatgc acataaaatt
     2341 gtatcaaaaa aatacgatta ttcaattttt ataaaaaaga aggtggatta aatgcttaca
     2401 ttaataaaat tgaaag
//
LOCUS       DAKTIP010000269         2384 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.274, whole genome shotgun sequence.
ACCESSION   DAKTIP010000269 DAKTIP010000000
VERSION     DAKTIP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.274"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..828
                     /locus_tag="O0305_002651"
     CDS             <1..828
                     /locus_tag="O0305_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275751.1"
                     /translation="SIIKLLPEKISKLSNGEVIFDGKRIDTLNEKQLPDIRGNDIAMI
                     FQEPMTALNPVFTIKNQLVESIKSHKKISKKEANKLAKDLLKKVGIARQDEILNSYPH
                     QLSGGMRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMI
                     THDLSVVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLE
                     TIEGIVPSIQAFHVNKCRFANRCNKKLDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            831..1811
                     /locus_tag="O0305_002652"
     CDS             831..1811
                     /locus_tag="O0305_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCY0275752.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAQDMCKKSMPELKDIGNEHQVACFYVDKVGDLND"
     gene            1804..>2384
                     /locus_tag="O0305_002653"
     CDS             1804..>2384
                     /locus_tag="O0305_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0275753.1"
                     /translation="MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFT"
BASE COUNT          918 a          326 c          400 g          740 t
ORIGIN      
        1 tcaattatta aattattacc agaaaagata tctaaactaa gtaatggaga agttatattt
       61 gatggtaaac gaatcgatac gctcaatgag aagcaattgc cagatattcg aggaaatgat
      121 attgctatga tttttcaaga acctatgact gctttaaatc ctgtatttac cataaaaaat
      181 caacttgtgg aatctataaa atcacataaa aaaatttcta aaaaagaagc aaataaatta
      241 gcaaaagatt tactaaaaaa agttggaatt gctagacaag atgaaatatt aaatagctat
      301 cctcatcaat tatctggtgg tatgagacaa agagtaatga ttgcaatggc catttcatgt
      361 tctcctaaat tattaattgc tgatgaacct acaacagcat tggatgtcac gattcaagcg
      421 caaatattag acttattaaa agaattgcaa aaggaaacgc aaatggcaat tatgatgatt
      481 acacatgatt tgagtgtagt tgctgagttt tgcgataaag tcttagttat gtatgcaggt
      541 caaattgtag aatttggagg cataaaagaa atactacaca atccgaaaca tccttatacc
      601 caaaaattat tatcaacaat tccaaaactt aaagaagagc agaaacgact tgaaacgata
      661 gaaggaattg tgccatcaat ccaagcattt cacgttaata agtgcagatt tgcaaataga
      721 tgtaacaaaa aactggatat ttgtaataat caatctccta aaatgcatgt ttgtgaagat
      781 gtcattgtac gttgtcattt gtacaaaaat gaatataagg agatataaga atggaaaata
      841 ttttagaagt caaccaaata aaaaaatact acaaaattaa aactggatta ttacaaaaaa
      901 ctcagtacgt taaagctgtt gatgacgtat cgttttcaat aaaaaaagga caaacttttg
      961 gattagtagg agaatcgggt tgtggtaagt caacgttagg taaagtgatt atcaggcttg
     1021 aagatgcaac ttcaggctca ataattgtta atggtgaaga tataacaaga ttacaaggta
     1081 aaaaactcag aaaatcacga caacaatatc agatgatatt tcaagatccg tatgcatcat
     1141 tgaatccgat gcaaatggtt ggagatatca tttcagaacc tattttaaat tataaaaaat
     1201 tgccaaaaga agaaataaaa aaagaagtac tatatttatt aaaatgtgtt ggcctaagtg
     1261 aagatgcata ttataaatat gcacatgaat tttcaggtgg acagagacaa agagtgggaa
     1321 ttgcaagagc attggctttg cgtccgagtt taattgttgc tgatgagcct gtaagtgcat
     1381 tagatgtatc tgttcaatct caagtactga atttattaaa agatttacaa gaacaattta
     1441 acttaagcta tttatttatc gcacatgatt taagtgtagt aaaacatata agtgatgtca
     1501 ttggagttat gtatttaggt catatagttg aaatcgcatc tgataaagaa atttatgaaa
     1561 atcccaaaca tccatataca aaagcgttga tttcatcaat accacaaatt gataaacata
     1621 ataacaatag aattatatta aaaggagaat taccttcgcc aagtaatccg ccgcaaggtt
     1681 gtccttttca tacaagatgt ccaattgctc aagatatgtg caaaaaaagt atgccagaat
     1741 taaaggacat tggtaatgaa catcaagttg cttgtttcta cgtagataaa gtaggtgatt
     1801 taaatgatta aattaatact taaaagatta gggttaatga tcccgttact aattttaatc
     1861 tcaatagttg tattttcatt agcaatcata caaccaggag atccattttc agatctacaa
     1921 aacggtaaaa taaaacaaga agcaataaat gcacaaaggg aaaaattagg tctcaacgac
     1981 tcaataccac atcaatacat tagatgggtc aatcatgtta tacacggtga tttaggtgag
     2041 tcaatcaaat ataaaaggcc ggtaattgat gttattgagg aaagaattcc aaatacaata
     2101 ttactcggtg ctatgtcatt aattattact tatattatct catttgcttt aggaataacg
     2161 tcaggtagat attcttacag tttgacggat tatactgtgc aaatatttaa ttatttgatg
     2221 ttagccattc catcttttat tgcgggagta tttgcaattt ttattttttc ttttgaatta
     2281 caatggttcc cgtttcaagg ttctgttgat attaacctta aagaaggtac ttttgaatat
     2341 tatatgagta aaatatatca cacattttta cctgcattca cttt
//
LOCUS       DAKTIP010000270         2375 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.275, whole genome shotgun sequence.
ACCESSION   DAKTIP010000270 DAKTIP010000000
VERSION     DAKTIP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.275"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..297
                     /locus_tag="O0305_002654"
     CDS             <1..297
                     /locus_tag="O0305_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0275754.1"
                     /translation="NLKDFKDYKNGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYHI
                     PTKQAPELKLKGSGNLKGSSVGSKDLEFTFVENQEENIYFSDSVEFTPSEDDKS"
     gene            294..1618
                     /locus_tag="O0305_002655"
                     /pseudo
     CDS             294..1618
                     /locus_tag="O0305_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1618..1944
                     /locus_tag="O0305_002656"
     CDS             1618..1944
                     /locus_tag="O0305_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275755.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            1947..2234
                     /locus_tag="O0305_002657"
     CDS             1947..2234
                     /locus_tag="O0305_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275756.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT          982 a          320 c          437 g          636 t
ORIGIN      
        1 aatcttaaag attttaaaga ttataaaaat ggtgatattt catataatcc taatgtgcca
       61 agttattctg caaagtatca attgaataat gatgactata atgttcaaca gttaagaaaa
      121 cgatatcata ttccaaccaa acaagcgccc gaattaaaat tgaaaggatc cggcaattta
      181 aaaggctcat ccgtaggatc taaggatcta gaatttacgt ttgtagaaaa tcaagaagag
      241 aatatctatt tttcagattc ggtcgaattt acacctagcg aggatgataa atcatgagtc
      301 aaacggaata tcaaataaaa tctggcaata taaaaggtaa ctctgaagaa acaagtacag
      361 tatctaatat aagttatgaa atagaaaacg caaataacag tggtttaaaa caaaataaaa
      421 ttgataaaca aattaaaaag ttacaagaaa aaaataaatt ccctaaaaat ctttcatatc
      481 ttaaaagtta tacggacccc aaaacaggca cgactacaag cgccttttta aataaagaca
      541 ctggcaaagt tactttaggt atgacaggta ctaatgtaca caaagacgca atattaaaac
      601 aaacatttgg tgttccttct tatcaaggat atatagatgt gagtgaaacg ctaaaggata
      661 ttggggccga tgtcaatatt ggccttcatt ccgtcacaga taaagatcca cattataaaa
      721 atacccaaga ctttatcaaa aatatcaaaa aagactatga tattgatatt attaccggac
      781 attcgctggg cggtagagat gcgatgattt taggtatgag taatgatatt aaacatatcg
      841 ttgtgtataa tccagctcca ttagcgatca aagatgtgag cggcctttat gccgatcaag
      901 aagaacttaa aaaattgatt gaaaagtacg atggtcacat tgtaagattt gtgtctgatg
      961 aagacgaatt agatgcaggt gtccgcaatc atttatatga aactgctgga gaaaaaatag
     1021 tacttaaaaa tggagaaggc catgcaatga gtggtatttt aatgagcaga acacaggcta
     1081 taatcttagc tgaattaaac aaagttaaag gctaccaaga cgaaaataat aaagcattaa
     1141 aatccgttcg taaacaaacg aggcatagat tacataaagt agagacgtta agagcgaatt
     1201 ggattcaaac aacgggtgga tcactctctt cctcccaaca acaattatta gaagctttaa
     1261 cagcactaac cattgccgaa ggcttaaatc aattagtgaa tgaagaaagc caacatttga
     1321 aaaaatgtat cacgcgatgg cacataaatt tggagacaac tggaaaaaag cgcaagaagt
     1381 tggaaatgaa attggtgaaa aattaacctc tgaagaggtt atagatgaat taagaaaagg
     1441 tggcgcgtat gaaagtaaac ttgaaacaga tcccaaaaga aaaattgatg ataagataaa
     1501 gaaattaaat gatgtttata aaaattgtaa tggctatatc gcaaaaatta aacagagtat
     1561 cgaagcaatt gtttctaatg accaaatgtt agcgagccag attgatggga tgatgtaatg
     1621 tttactacgt ataaaaatat taatgaactt gaaaatgcct atgatgaaga aagaaaacaa
     1681 ttgaatgatg cattcaatca aattgatgaa ttaagacatc aaacacgcaa gaaatgtgaa
     1741 caaatgtatg atcatttctt atatctcaaa cataaaatga attatagtga agacgctatg
     1801 atcaggatga cacgtattat agaatctttc gatagagaaa cgaatcaacg tatccgacat
     1861 cacgaaatga aattagaaga ttataaagat gagttaagaa gagaatatct aaaacaatct
     1921 gacagaattg aaggagatga ataagcgtgg gacttgattt tagtggttta ccagatttag
     1981 cagtattgga acaaatgaag gaaaaagaac agattagtga ggttattgcg cctgaacatg
     2041 ttcgtatgca tcatgatcat caaaataagc tgaaaagtga tgagaaaata ttacttgacc
     2101 aaatggtaag tcatttcaaa aaatttgaag atgattttaa aaatgcggca caaggggctt
     2161 gggtgaaaaa tgccacagac gaattaaaag atattagtaa tgatttagaa aaaattcaag
     2221 atattaaagt ataaaaaggt attaagaaac acaataagta tataatccat ttaataataa
     2281 atgaattata tagttcataa tttcgactat aagtggctat tagccattat ttttcgggat
     2341 ctatgtcaaa tcggactaat gaattcaata atgga
//
LOCUS       DAKTIP010000271         2367 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.276, whole genome shotgun sequence.
ACCESSION   DAKTIP010000271 DAKTIP010000000
VERSION     DAKTIP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.276"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            82..852
                     /gene="fabI"
                     /locus_tag="O0305_002658"
     CDS             82..852
                     /gene="fabI"
                     /locus_tag="O0305_002658"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCY0275757.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(1047..2132)
                     /locus_tag="O0305_002659"
     CDS             complement(1047..2132)
                     /locus_tag="O0305_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCY0275758.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
BASE COUNT          848 a          339 c          403 g          777 t
ORIGIN      
        1 gtattgacga ataaaaagtt agttaaaact gggattagat attctatccg ttaaattaat
       61 tattataagg agttatctta catgttaaat cttgaaaaca aaacatatgt catcatggga
      121 atcgctaata agcgtagtat tgcttttggt gtcgctaaag ttttagatca attaggtgct
      181 aaattagtat ttacttaccg taaagaacgt agccgtaaag agcttgaaaa attattagaa
      241 caattaaatc aaccagaagc gcacttatat caaattgatg ttcaaagcga tgaagaggtt
      301 attaatggtt ttgagcaaat tggtaaagat gttggcaata ttgatggtgt atatcattca
      361 atcgcatttg ctaatatgga agacttacgc ggacgctttt ctgaaacttc acgtgaaggc
      421 ttcttgttag ctcaagacat tagttcttac tcattaacaa ttgtggctca tgaagctaaa
      481 aaattaatgc cagaaggtgg tagcattgtt gcaacaacat atttaggtgg cgaattcgca
      541 gttcaaaact ataatgtgat gggtgttgct aaagcgagct tagaagcaaa tgttaaatat
      601 ttagcattag acttaggtcc agataatatt cgcgttaatg caatttcagc tggtccaatc
      661 cgtacattaa gtgcaaaagg tgtgggtggt ttcaatacaa ttcttaaaga aatcgaagag
      721 cgtgcacctt taaaacgtaa tgttgatcaa gtagaagtag gtaaaactgc ggcttactta
      781 ttaagtgatt tatcaagtgg cgttacaggt gaaaatattc atgtagatag cggattccac
      841 gcaattaaat aatatcattc aacagctttg ttcacgttat tatatatgtg agcaaagctt
      901 ttttgctttt ataataatcg gactgatgga aaattatttg atatttcatt tgactgattt
      961 tttatgcaca aaaaaatcgc ctcaaaagct gtagtttcaa cttttgatgc gatagtatct
     1021 tatattctta aaagacatcc acagaattag tccttaacat tactgtttgc tttatcaata
     1081 atgcgttggc ggtatttgaa aatattacta acaaccgttt taagtacagc atataatggc
     1141 actgcaatca gaactaatgt aaagccacct aaatctcctg ctgctaaaat aacaacgata
     1201 attgttaaag gatggatact taaagattta cccattacat ttggtgtaat gatattacct
     1261 tcaagttgat gtgcaattaa tgtaatgata caaacccata taaatgtagt aggactatct
     1321 ataataccga gtattgctgc aggtgcaaat gataaccatg aacctaagaa aggaattaag
     1381 tttgcgacac cagcaaatag tactaataaa ggaatatatg gtaagtcaat aattgaataa
     1441 ccgatatata agaatatacc taaaataaca ctgacagtta cttgaccttg aatgtaagat
     1501 tttaatgtaa agtttaaatc agttaataaa tctacgaaaa atactttacg ttcacctttg
     1561 aaaaatttag caacagctgg gataaatttt tcatggtctt ttaacatata aattaagaag
     1621 aatggaacca taatcaataa gaagatggtt gaaattaatg atgtaatgta ctgtaatgaa
     1681 ttagataaaa tattagtaac gccatcaccc attgatttaa ccatatttgt aattctactt
     1741 gttacatctt caggtaattt atccatttga gctagtgcga atttaataat ttgctctgct
     1801 tctttttgta gggcaggtgt ctgactaatt aaattgttga tatttgaaat gatgattggt
     1861 gcaacaaacg caacaattaa gccgataata gcaaacaaag ctaacatgat tgttgttata
     1921 ctagcccatc ttggaaaacc gactttttca agtaagtttt gaaaaggtag acagatataa
     1981 aataaaaaac cactaattaa aaatggaaga aatacagaac cgatgattgt agctattgga
     2041 gtaaatactt cgtgcacttc cataaatagt ttgatgagaa tgaacagcat aatcagagcg
     2101 atgccagttc ggaaccaaac cttgtttaac ataaacgatg ttcctcctat ttttctaaac
     2161 ttagttatta tttagtatat cctaaatagc agtgcttaaa aaggttaata ttttttaaat
     2221 atacttaaat taaccattca ctttctattt taagatattt actttcaata gtttgtgaga
     2281 cttttcaatg actcgtaagt ctgcaattgg atcaaacagc accagtttat atgaataact
     2341 tattttaata ttaaacgtgt ttaaata
//
LOCUS       DAKTIP010000272         2363 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.277, whole genome shotgun sequence.
ACCESSION   DAKTIP010000272 DAKTIP010000000
VERSION     DAKTIP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.277"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            187..363
                     /locus_tag="O0305_002660"
                     /pseudo
     CDS             187..363
                     /locus_tag="O0305_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(341..442)
                     /locus_tag="O0305_002661"
     CDS             complement(341..442)
                     /locus_tag="O0305_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275759.1"
                     /translation="MQGFKEKHQELKKALCQIGLMRSISEVKQLNIA"
     gene            complement(565..660)
                     /gene="pepA1"
                     /locus_tag="O0305_002662"
     CDS             complement(565..660)
                     /gene="pepA1"
                     /locus_tag="O0305_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCY0275760.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            863..1006
                     /locus_tag="O0305_002663"
     CDS             863..1006
                     /locus_tag="O0305_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCY0275761.1"
                     /translation="MTRERRSFSSEFKLQMVRLYKNGKPRNEIIREYDFTPSTFVNGG
                     YKM"
     gene            1610..1993
                     /locus_tag="O0305_002664"
     CDS             1610..1993
                     /locus_tag="O0305_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275762.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKYKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGAISVISALTGGLVGAGVGGFLGSIAASNIDTSKG
                     IYIKLKTKKNAAGEYVLTGEKWGYQ"
     gene            2004..2180
                     /locus_tag="O0305_002665"
     CDS             2004..2180
                     /locus_tag="O0305_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275763.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            2182..>2363
                     /locus_tag="O0305_002666"
     CDS             2182..>2363
                     /locus_tag="O0305_002666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275764.1"
                     /translation="MKQISIGYEEIYGDRIFHSHFLKEHEVKNWKTVNQIIEKEHLDK
                     NEYEAEPPLFFFSGSL"
BASE COUNT          896 a          314 c          406 g          747 t
ORIGIN      
        1 tattgtgatg aaacaaaaat ataagtcatt agatgagttt aactatgtta taaatatttg
       61 tagtatctat aaaaatctcg acactattaa aatgataaag tgccgaggtt ttcttactta
      121 tttagttaat tcaaagttta tgccagattc ataagaattt gtgacacttt taatagtgta
      181 accattgatt attacaattt atcaaatggt cctttagaag gtataaataa caaaataaaa
      241 ttaattaaac gtacatcttt tggttatgga agttacaatc atttgcgcaa tcgaatatta
      301 ttatgttcaa aactttacgc tccaaaaagt aaaaaggaag ttaagcaatg tttagttgct
      361 taacttcgga tattgaacgc atcagtccaa tttgacatag agcctttttt agttcttgat
      421 gtttctcttt aaaaccttgc atattttaca aaaagaaaaa ttagcagtat aattaagaca
      481 acgaaaataa gtatttactt atacaccaat cccctcacta tttgcggtag tgaggggatt
      541 tttattggtg cggctatatg tcacctattt tgtattgcgt ctacttagcc aataagaaaa
      601 aaacgcaatg gcacagccac tgatgactgg tgctatgatg tgaacgaaaa taagcatcac
      661 cttatacacc tcctctctgc gtctaaattg acgcctgaga gataggcgac tctactatta
      721 tatcatcggc aaatatacaa gcacagtcac ttgcttctga taagttatat gattctagct
      781 gatagattga atcgtctaca cttaattgga caaattctat gagaatagat attgttaatt
      841 taagaaagta ggcgatttta ttatgacaag agaaagaaga tcatttagtt cagagtttaa
      901 gttacaaatg gttagattat ataaaaatgg taagcctagg aatgaaatta tacgcgagta
      961 tgatttcaca ccttcgacgt ttgtaaatgg cggttataaa atgtaggaaa atggataaag
     1021 caacatcaaa acacgggtac attcaatcac caagataact tatcggatga agaaaaagag
     1081 ctgattaaat tacgcaaaga agttcaacat ttaaaaatgg agaacgatat tttaaagcaa
     1141 gtagcgctga ttatggggca aaaatagaag tcattcaaaa gaatgcacat caatattcag
     1201 tatcagcaat gtgtaaagtc ctgataatac taagaagtac ctattatgat tctataaaaa
     1261 gaaaagataa taaaatcact aaagatgatt caaacataga acatgccgtc ataaatattt
     1321 ttaattctaa tagaaaagtc tttggtacaa gacgaattaa aaatcattta aatgacaagg
     1381 gtctcactgt atctggacaa aagataggtc gatcatgaaa aaatctagtt tctgtttata
     1441 cgaaatctaa atacaaaaat catctaaaag aaactaatga aaaacgaatt aaaaatcttt
     1501 attattagct gctggtgtat tatttgttag tccaatttca ttatcttata attcagatgt
     1561 agctcatgct gaagataagt tagaccattc tcaagcaaag gtaatatatt tgagtaacca
     1621 aaatttattt gatgaacttg agaaaaaagg ttataaactg gaagatatat ttacaaaaga
     1681 agaaataaaa aaatataaag ctgaagacca attgagagcg ggtaaaactc aatatgtaga
     1741 aacaggtaaa gatactgcaa cattatatct ttcttctgca tatacaaaaa caatagctgc
     1801 tttaggtgcg ggggctatta gcgttatttc tgcactaaca ggaggtttag ttggcgcagg
     1861 tgttggtggt ttccttggat ctatagctgc atcgaatatt gatactagta agggaatata
     1921 tataaaatta aaaactaaaa agaatgcagc cggggaatac gttctgacag gagaaaaatg
     1981 gggatatcag taagggtgat tttatggata gattaaaata ttcacttaaa gttggaattt
     2041 tagcattatt attattttgt actttaaatt atttagttcc aatgcaaagc aatgcttttt
     2101 caataattat atattcggca atttttgctg tgttacttat gcttttagtt tatatatttt
     2161 taggaatttt aaagaaatga catgaaacaa attagcattg gctatgaaga aatctatggg
     2221 gatagaattt ttcatagcca ttttttaaaa gagcatgaag taaaaaattg gaaaaccgtc
     2281 aatcaaataa ttgaaaaaga acacttggac aaaaatgaat atgaagcgga accgccgtta
     2341 tttttctttt caggatcgtt aga
//
LOCUS       DAKTIP010000273         2319 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.278, whole genome shotgun sequence.
ACCESSION   DAKTIP010000273 DAKTIP010000000
VERSION     DAKTIP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.278"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(6..620)
                     /locus_tag="O0305_002667"
     CDS             complement(6..620)
                     /locus_tag="O0305_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCY0275765.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            complement(637..1707)
                     /locus_tag="O0305_002668"
     CDS             complement(637..1707)
                     /locus_tag="O0305_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCY0275766.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTLIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            complement(1719..2228)
                     /locus_tag="O0305_002669"
     CDS             complement(1719..2228)
                     /locus_tag="O0305_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275767.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
BASE COUNT          739 a          428 c          299 g          853 t
ORIGIN      
        1 actatttaat ttattaattt atttctaagt aataacgttg ataaaataat gcaaaataat
       61 ccaccaataa tgccggcaat aatatctgtt ggataatgta cacctagata tacacgtgat
      121 atggaaatca ataaaatcat agctgcacat aaccctataa gaataccttt tgaattacct
      181 tgatttaatc gatttaatag atagatacca cttccaaaat atgcagttga tcccatagca
      241 tgaccgctag gaaaactaaa tcctgttata tcaattaaac gcagcaatgt aggtctttct
      301 ctatcgaata tattttttaa tgctggattc aaaattccag ataatgccat tgttaatgca
      361 aaaaataatg cttcaatttt gtggcgcttt aacatgagat atgccacaag aagtaatgaa
      421 atacataaca ttgcccagac ttcacctact ttagtagccc caagcatgat agatgtcgta
      481 ataaagctct ctgatgaata tacaaactca taaacttcat tatcaatcca tttcccaagt
      541 cttgattcgt ggaaaaacgc gataattcca aaaaccaatg taaaaacgat gagcaaagag
      601 atacgtttcc attgactcat aaagcattac cccccattaa ttcaatgcgt ctttaatcat
      661 cttatcaaat aaagcttctt tcgtataact ttgttcatac gatttcatat tattgataat
      721 tcgagttctt tcctgttcca tttcatttag ttcttgtaat aaaatttgtg ctgttaattg
      781 ttcttcatca atcgctttag catatccttt atcagcaaaa tgatttgcat tgtcaatttg
      841 gtcgcctcgg gattgatcta aacctaatgg tactaataac attggtatac gtaatgttaa
      901 gaactcataa atcgcatttg atccagctct acttattact gtatccgtaa ttgctaataa
      961 atctgttaaa tcctctttaa caaattcata ttgtatatat cctgattttt taacttgagc
     1021 atcttttaat cctttaccag ttaaatgtat cacttgatat tgttgtaata atgcatctaa
     1081 gttttcgcga ataatgctat ttaatttttt acttcctaag cttccaccca taacgagtaa
     1141 aactttttta ttttcattaa agcctgttaa ttgataacca ttatgtgcat taccattttt
     1201 taaatcttct cgaattgttg ctccaataaa atcagctttc tctttaggta agtagtttag
     1261 cgtttcttca aatgttgtat atattttctt ggcaaattta agtgctatct tattcgctaa
     1321 tcctggtgtt aagtcagatt catgaataag agttggtata tttaatgatt tggctgcaat
     1381 aacaacaggc acagatacaa atccaccttt tgaaaataat agatcaggtt tttctttttt
     1441 caaaacttta cgagcatcaa gaataccttt caatacttta aatacgtctt tggcattttc
     1501 taaagaaata tatcttctta atttaccact cgaaatagga taatacttaa tttctggtag
     1561 ttgtgattca atcatttctc tttcaatacc atttttagaa ccaatataaa gcgcttcata
     1621 accttgtgat aatgcagttg gaattaaact taaatttact gatacgtgtc caactgttcc
     1681 ccctccggta aatgcgattt tcgtcattaa tattacctct attcttttaa atttttataa
     1741 tatgcataaa atggttcacc tttatcatat ggcggatatt ccattaattg ttcaccgacc
     1801 ttatgaaatc caaatttggc aaataaacct tgtgcaggtt tgtttaacgc aaaggtgtcc
     1861 gttaaaataa cttctgcacc acgtgcttta actacatcaa taacataatt gaataattct
     1921 gtagcagctc ctttatattc tttcgaacca gttaatcgat gaataacaaa ggcgccttct
     1981 ctatttactg gccagtcaat gtcatcatac cattctgctt ggtcttggtc gacaacaata
     2041 aagccataaa ttttgtcatt ttcctctaat acgtacaaat aatctttagc aatatcttct
     2101 tcaaaatgtt ctaaaagtgg gtactgatcg tcccattgct cgttgtcgtg ttctttcatt
     2161 aattcttttg cttcttctac tagatttaag atttgatcta aatctgacat tttacctaga
     2221 cggatcatgt acgtcactcc ttatatcatt atgttaaaca tgtttttcag tacatgttaa
     2281 tcttacacac tattaacatg ctttaaacct ttattatga
//
LOCUS       DAKTIP010000274         2313 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.279, whole genome shotgun sequence.
ACCESSION   DAKTIP010000274 DAKTIP010000000
VERSION     DAKTIP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.279"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..108
                     /locus_tag="O0305_002670"
     CDS             <1..108
                     /locus_tag="O0305_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275768.1"
                     /translation="YYKYRKFGILPETEYEINKVKEIEQYVKEQEEAEQ"
     gene            119..619
                     /locus_tag="O0305_002671"
     CDS             119..619
                     /locus_tag="O0305_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275769.1"
                     /translation="MNFEEKLSEMYNKIANEINEMIPVEWEKVYVIAYVDDGGGQVIF
                     YYTKPRNDELYYYSSIVEDYNVLEEIFDDLWMELYRSFKKLRNIFKEESLEPWTSCEF
                     DFTKEDKLKVSFDYIDWKNTEFDQLGQENYYNYRKFGIIPEMEYEMEEVKQIEQYIKE
                     QEEAEQ"
     gene            630..1125
                     /locus_tag="O0305_002672"
                     /pseudo
     CDS             630..1125
                     /locus_tag="O0305_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
     gene            1136..1627
                     /locus_tag="O0305_002673"
     CDS             1136..1627
                     /locus_tag="O0305_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275770.1"
                     /translation="MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFY
                     NYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEF
                     DFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEMEEVKEIEQYIKE
                     QEE"
     gene            1837..>2313
                     /locus_tag="O0305_002674"
     CDS             1837..>2313
                     /locus_tag="O0305_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0275771.1"
                     /translation="MTTKENIDILRKPGAQALSLASLFMILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVIINVLTTITAVFATC
                     NLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTH"
BASE COUNT          937 a          226 c          429 g          721 t
ORIGIN      
        1 tactataagt atagaaaatt tggaatttta ccagaaacgg aatatgaaat taataaagtt
       61 aaagaaatcg agcaatatgt taaagagcaa gaagaagctg aacaatagag gcgataacat
      121 gaatttcgaa gaaaaattaa gtgaaatgta caataaaatt gcaaatgaga tcaatgaaat
      181 gataccagta gagtgggaaa aggtatatgt aattgcatat gtggacgatg gaggtgggca
      241 agttatattt tattatacca agcccagaaa tgatgagctg tattattatt cgagtatcgt
      301 tgaagattat aatgttttag aagaaatctt tgatgattta tggatggaac tttatagatc
      361 atttaaaaaa ttaagaaata tatttaaaga agaaagtctt gaaccatgga catcatgtga
      421 atttgacttt acaaaagaag ataaattaaa agtttcattt gattatattg attggaagaa
      481 tacagaattt gatcaattag gccaagaaaa ctactataac tatagaaaat tcggaattat
      541 accagaaatg gaatatgaaa tggaagaggt taaacaaatc gagcaatata ttaaagagca
      601 agaagaagct gaacaataga ggcgataaca tgattttcga agaaaaacta aatgaaatgt
      661 acaacgagat tgcgaataaa attagtagca tgataccagt agaatgggaa aaggtatata
      721 caatggctta tatagatgat ggaggaggtg aagtattctt taattatact aaacagcgat
      781 gaattgaatt attacaccga tatacctaag gagtataaca tttctgtgca agtatttgat
      841 gatttatgga tggatttata tgatttgttt gaggaattaa gaaatttatt taaagaagaa
      901 ggactagaac catggacatc atgcgaattt gattttacaa gagaaggtaa attaaaagtt
      961 tcatttgatt atattgattg gataaattca gaatttggtc aagtaggtcg acaaaattac
     1021 tataagtata gaaaatttgg aattttacca gaaacggaat atgaaattaa taaagttaaa
     1081 gaaatcgagc aatatattaa agagcaagat gaagctgaac tataggggcg ataatatgac
     1141 tttcgaagaa aaattaagtg aaatgtatag cgagattgcg aataagatta ttagcatgat
     1201 accggtagag tgggaaaaag tatatgcaat ggcatatatc gatgaagaat gtggagaagt
     1261 gttttacaat tatactgaac caagcagtga tgaattattt tactatacaa gtgtgataaa
     1321 gaagtataat ttattgaaat caagctttat ggattcagta tatgagttgc atgatcaatt
     1381 tgaggaacta agagaagtat ttatagaaga aggtcttgaa ccatggacat catgcgaatt
     1441 tgattttaca aaagaagata aattaaaagt ttcatttgat tatattgatt ggataaatac
     1501 agagtttgat caattaggcc gtgaaaatta ttatatgtac aaaaaatttg gtgttttacc
     1561 agaaacggaa tatgaaatgg aggaggttaa agaaatcgag caatatatta aagagcaaga
     1621 agaatagaca atcttaatgt aagattaaac aataaagctt tgtttagtct ttttagcgtt
     1681 taagtaaaaa acaatagaga ccataaagtt gtcatcaaat aataatataa agataatttt
     1741 aggcttttat acttttaatc gaaaagaatc taagtactta taactttaaa tacttaatat
     1801 gctattacaa aatcaatgta taggagatag gtaaatatga ctactaagga aaatatagac
     1861 attcttcgaa agccaggtgc acaagcctta agtttagcat cattatttat gatacttttt
     1921 tcatgtctaa ctttcttttt tggtttagat tatgaaaggt ttccaaatta tttaaagata
     1981 acgacaatta tagaattaat aattatcata attagtttac ttcaatggat tagatttata
     2041 gatttcgaaa aggaaagcgc acagaaatat aagaaaatat atgcccgatt tttagttatt
     2101 ataaatgtgc taactactat caccgcagta tttgcaacat gtaaccttta ttattttgtt
     2161 gctgtacaaa atcattatga cctattcaat tattggttga tgggtacgat ttcaatcata
     2221 attagttatt tgttattagt aattggcgga atgttcacgt tattaaaatt acctaaagta
     2281 acaaaacgtt ggggtggtaa aactaaaaca cat
//
LOCUS       DAKTIP010000275         2308 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.280, whole genome shotgun sequence.
ACCESSION   DAKTIP010000275 DAKTIP010000000
VERSION     DAKTIP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.280"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            371..562
                     /locus_tag="O0305_002675"
     CDS             371..562
                     /locus_tag="O0305_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275772.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(701..886)
                     /locus_tag="O0305_002676"
     CDS             complement(701..886)
                     /locus_tag="O0305_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275773.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(822..1103)
                     /locus_tag="O0305_002677"
     CDS             complement(822..1103)
                     /locus_tag="O0305_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCY0275774.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            1392..2087
                     /locus_tag="O0305_002678"
     CDS             1392..2087
                     /locus_tag="O0305_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0275775.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
BASE COUNT          808 a          343 c          407 g          750 t
ORIGIN      
        1 aaagaacgga agtttaatag cttatatgat tcttggagct aagacagcat gcgttcattc
       61 atgccattat taatataagc accgcaacaa aaaagcttct aatgtgatac aggaacctca
      121 tattccgtat catgttagaa gcttttaatg tctaaagaac atctacattt tatcatattt
      181 tctgacttat taaactttta tataattaaa tatttcttaa ttttccaaaa tagtgataaa
      241 tttgtgaaat acatcacaaa tccctttatt tatttggaaa ttcatgtaat attagacttg
      301 taagaagtta ataaatagag agagacgaga gagtttatat aaatactata taaacattgg
      361 agtgatgatt atgagaaaag agattgaagc gcttattttc tcagacgtaa gtagctatga
      421 tatttacgtg aacactggtg taaatcaagg attaattggt gacatcaaag atggttacct
      481 aactattgat tctatgcctt acattgatgc tgagcgtttg tatcactttg ctatggaacg
      541 taaatcgtta gtcactaact agttcttatt gccaatgatt actaccccta gtcggcggca
      601 attgaagtgt gattgatgta acttgccctc gttggtgagc aattgagggc agaccccttt
      661 aattaagtaa accctaactc cccacaaatc tggaacgata ctaaaagcca cgtcctatat
      721 tggatgtggc tttagtcata cttatattat ttttaaaacg attacctaca agatttacat
      781 ataaaattct atcatgtctg ctatatataa acgaaatgat attagaatcc ttcattttca
      841 acttgtttaa aattacgttt aatgccgtta taagaatata caacattata taattttact
      901 tgggcgtcat ggacacttga gaagggcatt cttacatcgt ccatattttt aacttcgtgt
      961 cgaaatgctt tttcagcttt ttgtctttct gcaagagaca tacttagcca ttcttctttg
     1021 ctaaataaat cttcgaatcg aaaatcaaat gcggttcttt tactttctgc tcttaatgct
     1081 aaaacacgct catgatatgt cacgttttat tcctccctct aatcattcaa tcgaatgata
     1141 aatcacagta ttaactttat tactaatata atgtttttac aataaagaag tgacgatttt
     1201 tcaacatttt taaaaataaa aaatataagc aacgattaat tgtatgtttc gaatggcttg
     1261 taatgtaatt tatctatgaa aatgaagcga gttattatga ccgttatatg aattgtgata
     1321 cgctttttaa aagtaatatg tatttatgat ttaccctgaa aaaataaaaa gaatcaaagg
     1381 agtcgatcat tatgacagta ttaacagata aagtagcagt agttacaggt gcaggtagtg
     1441 gtattggaga agcaattgca acattactac atgaagaagg ggcaaaagtt gtcttagcag
     1501 gtagaaataa agaaaaatta caaaacgtag cgaatcaatt gtcacaagat agtgtgaagg
     1561 tagtgccaac agatgtaacg aataaagaag aagtcgatga attgataaaa attgcacaac
     1621 aaacattcgg tggtttggat attgttatca atagtgcggg gcaaatgttg tcgtctaaga
     1681 ttactgatta tcaagtagat gagtgggata gtatgattga tgtgaatatc aaaggcactt
     1741 tatatacggc acaggctgca ttaccaacta tgttagaaca atcaagtggc catcttatta
     1801 acattgcatc tatttctggc tttgaagtaa cgaaaagtag tacgatttat agtgcgacga
     1861 aagcagcagt tcacactatt actcaaggat tagaaaaaga gttggcaaag acaggcgtta
     1921 aagtaacaag catttctcca ggaatggtag atacagccat aactgccgca tacaatccaa
     1981 cagatcgtaa aaaacttgaa ccacaagata ttgcagaagc agtattatat gcattaacac
     2041 aaccaaagca cgttaacgtg aatgaaatta cagtgcgtcc tgtataaaaa ggtgtttgag
     2101 gtctggggta taggcattta atgtaatagc tatcgatttt gttaatgtta gtaaggcaat
     2161 aattacatga gggcataata ggcaattatt ccttgtacaa ttctcagtaa ttgctgattt
     2221 taacaatcaa gttaatgaag gcgacgatgt tagtgttatg gtttataatc tgtaatgttc
     2281 ataattatag attataaaaa ttttttca
//
LOCUS       DAKTIP010000276         2302 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.281, whole genome shotgun sequence.
ACCESSION   DAKTIP010000276 DAKTIP010000000
VERSION     DAKTIP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.281"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(134..865)
                     /locus_tag="O0305_002679"
     CDS             complement(134..865)
                     /locus_tag="O0305_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCY0275776.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(867..2180)
                     /gene="murT"
                     /locus_tag="O0305_002680"
     CDS             complement(867..2180)
                     /gene="murT"
                     /locus_tag="O0305_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCY0275777.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLYLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
BASE COUNT          691 a          456 c          293 g          862 t
ORIGIN      
        1 attcactaga acaaaaaaac acacatcaat ttggttgttt ccatccaaaa taatgtgtgt
       61 gtttatacat atatgattaa ttactaaact tcatcgttta tcaatattaa atccattgat
      121 gcgatgttca gagttaacga gatttcttct gtctatttgc tctgtctatt aatacttgtt
      181 tcgcttgtat ttccgcttca ttatctattt ctttaggctc aaacggaata cccttacgtt
      241 cacaagcttt ttctaacaga taatcagtga tttcgtaatt tttaggtaaa attggtccat
      301 gtaaataagt acctaataaa tttttataat gaatgccttc ttttttatct tcatcattat
      361 taccataacc aaaagtaaca tgaccaagtg taccgaaatc atgatatgtt ctaccaccgt
      421 gattttcaaa acctacaata gttccaaaag tatcactttc gataacaata tctcctgtta
      481 atcggtttgt ctttgattca gtataaaaat ctaaaatacc taacccttct aattctgtac
      541 catcaggcgt gatatatttt ttccctaaaa attgatagcc tccacaaatc gttaatcccg
      601 gcataccatc ttcaatcgct tctttaagtg gtgtcttaat tttacttaat tcttttgttg
      661 ctaatgcttg ttctctatca cttccaccac cgataaagaa aatatcacat tcatcaaagg
      721 taataccttc tgtttcattg atttctacga cattaacttt aatatttcgt tttttagcac
      781 gttgtcttaa agcaataata tttcctatat cactgtataa attcaattta tctgacataa
      841 aatgataaat agtcaattca tgcatattat gattgacctc cttcaaacga acggtttaat
      901 tgttcaagca taggcgctaa tgatgtatag tttggtattg caactgtgaa acctttataa
      961 tccatagtct ttgccgttgc tttataaata tcacgctcaa ctataattgg tacttcaacc
     1021 tctgctaact tcaatcgcaa ttgaagttct tctgctcgtg tacctgtcac gatgatagct
     1081 tcaatttgtt gcttagataa tttttcaaaa tctgcatcat aaatccatga agtatctcga
     1141 ccatctgcag cgttatcatt tagcgaaata acatacactt tttcgccttc taattgttca
     1201 ccaactgata aacttgcatt cattcctgca ggatttttag ctaaattgat catcgcttct
     1261 tttcgttctt ttttaaagta ctgcatacga ccattgtctg atgtatacgt ttcaaagcca
     1321 tttttaattg tttgttcatt taaccctagc tctcttaaaa cagtatatgc tgctaacgcg
     1381 ttataagcgt taaagtcacc tgcaattttc atatcatatt tttcatcatt gatatttaaa
     1441 tataaaaacg gtgccacatc aaaacttgat atttcatatt ttgcttgctc tcgtttgaaa
     1501 ccacactgac agtgataatg accaatttga ttataatgaa tataatcgta ttgcaataag
     1561 cgaccacagt ttggacaata tctactttca ttcatcgtac tttgttcaaa ttcatgggca
     1621 tgtgctttca taccatagta cacaatcgta tcacttgcga ttttcaaacg actcacaaat
     1681 ggatcatcag catttagcaa taatttgatg cctttattac taattgtctc tgcaatgtta
     1741 ttaaccataa tatcaatttc accgaagcga tccatttgat ctctaaagaa attagtaaat
     1801 accatcattg aaggtgtaac ttcttttaac acacgtggaa tcgaaccttc atcaatttcg
     1861 attaccgcaa ttttagtctt aggtgttgat tgcatgatga atgcagaagt tatacctgca
     1921 gccatattag caccttcatt attgtgtata atttgaatat tatttgcttt taaagtatgt
     1981 ccaattaagt ttgaagtcgt tgttttacca tttgttccac tgataaatac aatatcatca
     2041 acttgctctg ctaattttct taatatatct gtatccactt ttctagcgat ttgtccaggt
     2101 aaatctgttc ctcttttacc tactgctcta cttgctttac gcgccaattt cgctagatgg
     2161 attgccgtcc actgtctcat gtgtttcctc ctcaaatttc cactcgcatc attataacat
     2221 gacaaggcaa cttcaaaaaa gtttctcaat cacaaattga tacagtgttt ctaaattata
     2281 atcattctca tttagataat ta
//
LOCUS       DAKTIP010000277         2267 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.282, whole genome shotgun sequence.
ACCESSION   DAKTIP010000277 DAKTIP010000000
VERSION     DAKTIP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.282"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1184)
                     /locus_tag="O0305_002681"
     CDS             complement(<1..1184)
                     /locus_tag="O0305_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCY0275778.1"
                     /translation="MAKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFL
                     YFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKT
                     NLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLN
                     TKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKS
                     KF"
     gene            complement(1177..2232)
                     /locus_tag="O0305_002682"
     CDS             complement(1177..2232)
                     /locus_tag="O0305_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCY0275779.1"
                     /translation="MVGIALITTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSP
                     DKEEKLAKNNILFFLTWAVPAAISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIP
                     KLTGEIPSYINFGLMNYQNASYLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVF
                     IPGGRGGAILLILYGLFAFILITFKRGIPIAVKSIMYIFALSISSVLIYFLFTKGSNT
                     RTFSYLQGGTLNLEGTSGRGPIYEKGIYFIQQSSLLGYGPFNYYKLIGNIPHNIIIEL
                     ILSFGLLGFFIIMICILLLVYKMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSE
                     FWFVLFYFITKGRRHHG"
BASE COUNT          893 a          343 c          294 g          737 t
ORIGIN      
        1 atgaatttag actttaaacc tataatattt aaaatcacat acttaataat gttgctgaac
       61 agcgttgtcc aaaagaatcc atttagacca aacgcaattg tcattaaaat agttatgaat
      121 ataaatgtaa tcgtgtgaag cgtcatataa ttcgcttgta atgttattga tgcgtgtttt
      181 gtatttaaag tttggatcat aatactcact gcattgaata aaacacctat attacctaaa
      241 ataataaact tcgaatataa acttgaatca atgttataca gtaaagagac aataattatt
      301 gtaattggat aacatataat catgactaaa catagggcag ctatagcaat tagattagtt
      361 ttcaaatatt gcttctttat attgtcactt tcatttacag aaatatatga aagtactaca
      421 ttattaatcg gatatagaaa tgtagctaac attttcccaa taaatgttga aagaaatgat
      481 atagttacag ctgttccacc tataattggt aataagatta atctatctaa atagagattc
      541 aaattattaa ggctatttgt actcagtagc atcacataat cttttaccac attattatct
      601 tcactttgat actcgcctat agttaatccc cgtaatttaa ccaatgtata tatcgttgca
      661 aacaattcac tggtaataaa acaaacaatc cagttttggg ttaaataata tagaaatagt
      721 cctatcagca aacctaaaaa ttgaataaga gcaatataca aaatctgatt atattttaaa
      781 gtcatcctaa aaaatacatt cagataaatc cttaaacaca ttaaaatatt aagtagaatt
      841 aaaaagataa tatcgatgat gttcaaatta aaaaagtaaa gaaatacaat aattaaagct
      901 atactctcaa tcagaattga gattaaaagt atcgatgcaa atttccagta gtaatgattg
      961 gatttgtata gattcatatt aatcaatcgt atattgttaa gcgtattgcc taatacaaca
     1021 ctcgtgattg ttattattgt ataaatcgtt aaaatagaac cgaacgcttc attacctact
     1081 cgttgattaa taattggata agctaaaaat tgtaatccta aagctataag caacgtacca
     1141 attattgtct ttacgctatc cataataaaa actttcttag ccatgatgac gccgtccttt
     1201 tgtaataaaa tagaacaaca caaaccaaaa ttcacttaca actaaataat ttgaactaaa
     1261 cattaataat gtgattggat agattgctat aaacataacg agtaaatcta tagtgtttgg
     1321 atcatagttc ctaatcattt tataaactag tagcaaaatg caaatcatta tgataaaaaa
     1381 ccctaataag ccaaatgata gaatcaactc aataatgatg ttatgtggta tatttccgat
     1441 tagtttataa tagttaaatg gcccatagcc taataacgaa ctttgttgaa taaagtaaat
     1501 acctttttca taaatcggtc ctcttccaga agtaccttct aaattaagtg ttccaccttg
     1561 tagatatgaa aatgttctag tattcgaacc ttttgtaaaa agaaagtaaa tcaatacact
     1621 agatatgctt aatgcaaaaa tatacataat gctttttact gcgataggta ttcctctttt
     1681 aaacgtaata agtataaatg caaataagcc gtaaagaatt aataaaatag cacctccacg
     1741 ccctcctggt ataaacacaa tagggatatc aattattgta aatagaacat atatccactt
     1801 atgtttaact gaacctttca taatgaaata aatgcctaat ccggcagtaa atgctgaaag
     1861 gtacgaagcg ttttgatagt tcataagtcc aaaattgata tagctaggta tctcacctgt
     1921 aagttttggt attaaaatta caaaaataaa tgaaacagaa aatatgaaaa atactaattt
     1981 aaaaaatctt tctaccgtag ccttgtttat atatttaata taaataccac taattgccgc
     2041 tggaactgcc catgttaaaa agaatagaat attattttta gctagtttct cttccttatc
     2101 tggtgaaaaa taataaaaag ctagataaag caaacatata gcaattaata atattaaccc
     2161 tcgcggaatt tcttgggtga caattatctt ataaatagca aacaccgtag taattaatgc
     2221 tataccaacc atggttgagt aatacaccgg ctctataggg aacccta
//
LOCUS       DAKTIP010000278         2261 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.283, whole genome shotgun sequence.
ACCESSION   DAKTIP010000278 DAKTIP010000000
VERSION     DAKTIP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.283"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..176
                     /locus_tag="O0305_002683"
     CDS             <1..176
                     /locus_tag="O0305_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275780.1"
                     /translation="KHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMICA
                     FISRSNDRRKNSK"
     gene            440..820
                     /locus_tag="O0305_002684"
     CDS             440..820
                     /locus_tag="O0305_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0275781.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            817..1713
                     /gene="pmtA"
                     /locus_tag="O0305_002685"
     CDS             817..1713
                     /gene="pmtA"
                     /locus_tag="O0305_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCY0275782.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            1714..>2261
                     /gene="pmtB"
                     /locus_tag="O0305_002686"
     CDS             1714..>2261
                     /gene="pmtB"
                     /locus_tag="O0305_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCY0275783.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLVNYFVLSQS"
BASE COUNT          789 a          288 c          388 g          796 t
ORIGIN      
        1 tgaaacatct aacaaaaata tttgtagtac ttgcaattat tttatttatt attggttatt
       61 atttacaagc aactaatcat gaaagccaag gtataaaatt attattagca gcgattatgt
      121 ttatgatttg tgcttttata agtagaagta atgatcgacg taaaaatagt aaatagaatt
      181 gactgtttaa aacgattgtt tatgttgacg ctacggttgt cacgtagaga ttaaagacat
      241 tcgttttagc agtctttttt attgaagtag acaatgcaag attaaatggt agtgtcattt
      301 cattttattt tattcaactg ctagtaatac aatactcagt gttatttctg aagactggtt
      361 gataatgaga catgcatatt tacaaaactg tgtatattgt gtatattgta tatatacaga
      421 agttgatagg gggacgttga tgaaaataat tttaaaaaac aatagtgatt ttccgattta
      481 tgaacagatt aagcaacaag taaaacaaaa tattttaaag ggacatgttg ctcctggaga
      541 gcatttgccg tcaatgagag aacttgccaa agatcttcaa gtaagtttga ttactaccaa
      601 acgtgcttat gaagatttag agaaagacgg ttttgttaca acaattagag gaaaagggac
      661 ctttgttaag gagcaagata gttctatttt aaaagagaaa caatttttta ccattgaaaa
      721 tttggttaaa gaattggtta atgaagcgca agccatcgaa atgtcacttg aggaactgca
      781 ggatatttta acgttcattt atgaggagga atcatcatga atgccataga attaagtaat
      841 gttaattatt caagtgatca gtttaattta aagaatatct ctttcaaagt tcctcaagga
      901 ttcgtcactg gttttattgg tagaaatggc gctggtaaaa ccacaataat aaggttaatt
      961 atggatttgt atcaaccaca aactggtgtt attagagtat tagaagagga tatggctctc
     1021 aatccgatag agttaaagaa tagaattggg tttgtttact ctgaaaatta ttttaatgag
     1081 agatggacta cgaagcaact tgaaaaaatg attgcccctt tttatcgtaa atgggaccat
     1141 caagtatttg aattttatct tgaaaagttc gatttaccaa tcaataaatc aataaaaaca
     1201 ttttcgactg ggatgaaaat gaaattatca ctagcagttg cattttcaca tcatgctgaa
     1261 ttgtatattt ttgatgagcc tacttcaggt cttgatccct tagctagaaa tgagctattg
     1321 gagataattc agcaagaatt aattgatgaa aataaaacaa tattcatgtc gacacacatt
     1381 atctcagatt tagaaaaaat agctgattat atcattcact taagtgacgg tgaagttatc
     1441 ttaaatggtt caaaagaaca actgttacaa agatatcaag ttgtgagtgg tgctattgag
     1501 gaccttgatg atgaattagc atctctactt atttatgaag aacataagcg tacgggattt
     1561 ataggattaa cagaacatgc gcaagtattt aaagaaattt taggtcataa agtgaatatc
     1621 actacacctt ctattgagaa cttaatggtt tatcttgaaa agagaaaacc taaatatcat
     1681 gaaaacatca aattgatgga agaaggtttt taaatgaagg cattgttaat taggaatttt
     1741 aaattacgac gatatacact tatcatctat gttctattat taaccttata tcctttttat
     1801 ataatgcttg actcaacaaa gttcttttac ttattgcaat cttttataag tccaacaata
     1861 ttgattattt ggattttaga tgctggtcac ttatttagac tcaatcgtag attaggtggt
     1921 aatgattcat attattttta tatgagttta ccagtgtcaa aaaaacaatt gcttaatgcc
     1981 aactatatta catgcattgt tttaacatta attggaacgc ttgttattag tttatatgct
     2041 tatgaagcag atgtgattga accaaattca atctattttt caactgcata tgcatttgtc
     2101 atatctaact tcttgtctat accaattgca tttagtcaat ttacagaatt gcgtagagtc
     2161 aaagtgccat atggtatata cgtgtttact attatcattt tagttccatt tttattttca
     2221 attgcaatag tattggtgaa ttattttgtt ctaagtcaat c
//
LOCUS       DAKTIP010000279         2253 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.284, whole genome shotgun sequence.
ACCESSION   DAKTIP010000279 DAKTIP010000000
VERSION     DAKTIP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.284"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1300
                     /locus_tag="O0305_002687"
     CDS             <1..1300
                     /locus_tag="O0305_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HCY0275784.1"
                     /translation="SASEELYYSVEYKNTATFNKLVKKKSLNVVYNIPELHVAQIKMT
                     KMHANALANYKNDIKYINATCSTCITSEKTIDRTSNESLFSRQWDMNKITNNGASYDD
                     LPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKNLVPLNGFRGTEPEETGDVHDVNDRK
                     GHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGSKKTELLWVSKAIVQAANDGNQVINI
                     SVGSYIILDKNDHQTFRKDEKVEYDALQKAINYAKKKKSIVVAAAGNDGIDVNDKQKL
                     KLQREYQGNGEVKDVPASMDNVVTVGSTDQKSNLSEFSNFGMNYTDIAAPGGSFAYLN
                     QFGVDKWMNEGYMHKENILTTANNGRYIYQAGTSLATPKVSGALALIIDKYHLEKHPD
                     KAIELLYQHGTSKNNKPFSRYGHGELDVYKALNVANQKAS"
     gene            1323..2015
                     /locus_tag="O0305_002688"
     CDS             1323..2015
                     /locus_tag="O0305_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding subunit"
                     /protein_id="HCY0275785.1"
                     /translation="MAKLVTENISKRFKNQDVLKHINITLENNEVYGLLGINGAGKTT
                     LMKIICGILQQDSGEIKLDNRPMTRNDLHKVGSLIETPATYNHLSAQDNLKIVCLNES
                     VDFSEINSVLSLVNLNVDKKKKVKDFSLGMKQRLGIAMALIKKPEILVLDEPSNGLDP
                     YGIQELRELLKLLTEQGTSIIISSHILSEIQVLADHIGIIHEGELKYQQRNNKDENLE
                     EIFFKITKGDYK"
     gene            2012..>2253
                     /locus_tag="O0305_002689"
     CDS             2012..>2253
                     /locus_tag="O0305_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0275786.1"
                     /translation="MIHLKIEGIKFKNSFSMYVLLISPLVFLCFAIFTVLFAKSNTGT
                     ANSVSPYITLLFNIWPIAFIPIVLCMACNSLFKIEM"
BASE COUNT          860 a          302 c          418 g          673 t
ORIGIN      
        1 atcagcgtca gaagaactat attacagtgt tgaatacaaa aatacagcaa catttaacaa
       61 gttagttaaa aagaagtcct taaacgttgt ctataatata ccggaattac atgtggcaca
      121 gattaaaatg acgaaaatgc atgctaatgc tttagcaaac tataaaaatg atattaaata
      181 tatcaatgcc acatgttcaa cttgtattac tagcgagaaa acaatagaca gaacatcaaa
      241 tgagtcatta ttttcaagac aatgggatat gaataaaata accaataatg gtgcatcgta
      301 tgatgatttg ccaaaacatg ctaacaccaa aatagcaatc atagatacag gtgtgatgaa
      361 aaaccatgac gatttgaaaa ataatttctc gactgattct aaaaatttag tacctttaaa
      421 cggttttaga ggtactgaac cggaggaaac aggtgatgtt cacgatgtca atgataggaa
      481 aggacatggc acgatggtgt cgggtcaaac gagtgctaat ggtaagttaa taggtgttgc
      541 accgaataac aaatttacaa tgtatcgcgt gtttggtagt aaaaaaacag aactgctttg
      601 ggtatcaaaa gcgattgttc aagctgcaaa tgatggaaat caagtcatta atattagtgt
      661 tggtagttat attattttgg acaaaaatga ccatcaaaca tttagaaaag atgaaaaagt
      721 agaatacgat gcgttacaga aagcaatcaa ttacgccaag aagaaaaaat ctatcgttgt
      781 tgctgcagct ggtaatgatg gtattgatgt caatgacaaa cagaaactaa aattacagcg
      841 tgaatatcaa ggtaatggcg aagtgaaaga tgttcctgca tctatggaca atgtcgttac
      901 agtaggatct acagatcaaa agagtaatct atctgagttt tccaattttg gtatgaatta
      961 tacagatatt gctgcgcccg gaggatcatt tgcttattta aatcaattcg gtgtggataa
     1021 atggatgaat gaagggtata tgcataagga gaacatttta actactgcca ataacggaag
     1081 atatatttat caagctggaa cttcattagc cacacctaaa gtttcgggag cactagcttt
     1141 aatcattgat aaatatcatc ttgaaaaaca tccagataaa gcgattgaat tgttatatca
     1201 gcatgggaca tctaagaata ataaaccatt tagtagatat gggcatggtg agcttgatgt
     1261 gtataaagca ttaaatgtag caaatcaaaa agcaagttaa taaatcaaag gagtttttga
     1321 ttatggcaaa attagttact gaaaacattt cgaagcggtt taaaaatcaa gatgtattaa
     1381 agcatattaa tatcacttta gaaaataacg aagtttatgg attacttggt attaatggag
     1441 ccggtaaaac gacacttatg aaaattatat gtggcatact tcaacaagat tcaggggaaa
     1501 ttaaattaga taatagacca atgacacgaa atgatttgca caaagttggt tcgcttattg
     1561 aaacacctgc gacatataat catttaagtg cacaagataa tttgaaaatt gtgtgtttaa
     1621 atgaaagcgt tgatttcagc gaaattaata gtgttttaag cttagtcaat ttaaatgtcg
     1681 ataaaaagaa aaaggttaag gacttttctt taggtatgaa acaaagactt ggaattgcaa
     1741 tggcattaat taaaaagcca gaaattttag tattagacga accatctaat ggtttagacc
     1801 catatggaat ccaagaactt agagaacttc taaaattatt aacagaacaa ggtactagta
     1861 ttattatttc aagtcacatt ttatctgaaa tccaagtttt agcagatcat atcggtatta
     1921 ttcatgaggg tgagctaaaa tatcagcaaa gaaataacaa agatgaaaac ttagaagaga
     1981 tattcttcaa aataacgaaa ggtgattaca aatgatacat ttaaagatag aaggtatcaa
     2041 atttaaaaat tctttcagta tgtatgtttt attaataagt ccgctggtat ttctttgttt
     2101 tgctattttc acagtcttat tcgccaaaag taatacggga acagcgaata gtgtgtcacc
     2161 atatataact ttactattta atatttggcc aattgctttc atcccgattg tattatgtat
     2221 ggcttgtaat tcgttattta aaattgaaat gag
//
LOCUS       DAKTIP010000280         2243 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.285, whole genome shotgun sequence.
ACCESSION   DAKTIP010000280 DAKTIP010000000
VERSION     DAKTIP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.285"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1281)
                     /locus_tag="O0305_002690"
     CDS             complement(<1..1281)
                     /locus_tag="O0305_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCY0275787.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKFLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLE"
BASE COUNT          651 a          448 c          339 g          805 t
ORIGIN      
        1 ttccagatat tcatttacca attctatcat attttcagtt attgtttctc ctttgaaaat
       61 aataggaatc cctggcggat acgggacaat atgtcgcgcc agaactttac cttttgcctt
      121 tttcaaatca caccaagtaa catattcaaa gcgttttggt ttatagttac cttcagttgt
      181 taaaagttgt gtttgtttaa ctttagaaac tgattttttc ggtaaaatca tatcttcaat
      241 tttacgtaaa agcgaatcaa ataaatacgt atcatcatga tgccataacg gcaatatcgc
      301 taatgcttga tagtcgtccg ctaattctaa atagatatgt gcattcataa accaattttg
      361 aatatcatga cctgtaaaac cttcatattt tatcagcaac ttcaacggat catcaacttg
      421 aagcatttca aaacccttct tctccaaaca ttcgattaat tgcgctctct tatcaaaaaa
      481 cacggtacta tcatatgttt tatagaactc ggcagctgac tctaaactag ccataatcaa
      541 atacgaagga ctagatgttt ggaagtagct tagatattct ataatagttt ctctataagg
      601 tgcattttta tgaatataaa gtaccgagcc catcgttaaa gctggtaacg ttttatgaaa
      661 agattgaaca acatagtcag cttgataatt taatgtagaa tctggaaatc cttgcaatcc
      721 aaagtgcgcg ccgtgtgctt cgtcaatgag tacaggaata tttaattggt gcaaaaattt
      781 gataacctct tctacattaa atgtttcacc gtaatagtta ggataagtca acacagcaag
      841 tttgtgaccg tcattattca aacggcttaa attaacttta ttataatgat tcgttaacgg
      901 actttgatgc gtttcaataa aatgcccttc ttgttggcta atatcgagcg catgtaacac
      961 agatttatgt acatttcttg ccattaagat atcgcctttt ttctgtgaaa aagactggat
     1021 gacagataat attcctgaag tggtgccatt cactaagaaa taagcatcat aatctgaatg
     1081 tttctccacc tgcttcatac tttccaaaat gacttcttca ggatgatgta aatcatctaa
     1141 tccaggtatt tcagttttat ccattgtcat tgataattga gataaatgac cgatagtcat
     1201 atttttatga cccggaacat gcaaagaaat cgcttcttct tgatttaaac tttctaattt
     1261 atttaaaata ggttgcttca tgatatacgc ttcctttatt tacactgttt tggaattagt
     1321 tactttcaaa agtattaatt atatagtaac acttctttga caaaagttag tgttacttat
     1381 gcaatagctt gtctattgta taataattaa tttccttttt gtacttcgat ttaaaagata
     1441 ttagacataa aatctaaaaa cagcagtaag atgatttatg attaaaaact atcttactgc
     1501 tgttcacttt ttataatact tctgaatgtc ttcacttata cttctagtca cagatttaaa
     1561 taatcaaaag tgcacattat taaaatatca atttcacact caatgcggct catcgcattc
     1621 atttcttgtc tagcaacgtt ctactctagc ggaacgtaag ttagctacca tcctcgctaa
     1681 gaacctttct tgacttgtga caatcgcttg cttctttcct ctccttcggc tctcgcttac
     1741 tcatttagct ctactaaact cgttgcgctc ttttctcgtt tcgtcagatt caaacgtttt
     1801 cacttcgcca agccattttt ctttgtgttt actttttatt ttgacgtttt agacataaaa
     1861 aaagagacct cacggtctca acttgcctgg caacgttcta ctctagcgga acgtaagttg
     1921 gctaccatcg tcgctaaaga cctttcttga cttgtgacaa tcgcttgctt ctttcctctt
     1981 cttcggctct cgcttactca tttagctcta ctaaactcgt tgcgctcttt tctcgtttcg
     2041 tcagattcaa acgttttcac ttcgccaagc catttttctt tgtgtttgct ttttattttg
     2101 acgttttaga cataaaaaaa gagaccttgc ggtctcaatg cggctcatcg catccatttt
     2161 ttgcctggca acgttctact ctagcggaac gtaagttcga ctaccatcga cgctaaggag
     2221 cttaacttct gtgttcggca tgg
//
LOCUS       DAKTIP010000281         2232 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.286, whole genome shotgun sequence.
ACCESSION   DAKTIP010000281 DAKTIP010000000
VERSION     DAKTIP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.286"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            24..473
                     /locus_tag="O0305_002691"
     CDS             24..473
                     /locus_tag="O0305_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCY0275788.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            complement(568..903)
                     /locus_tag="O0305_002692"
                     /pseudo
     CDS             complement(568..903)
                     /locus_tag="O0305_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(1553..2044)
                     /gene="sak"
                     /locus_tag="O0305_002693"
     CDS             complement(1553..2044)
                     /gene="sak"
                     /locus_tag="O0305_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCY0275789.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
BASE COUNT          805 a          372 c          320 g          735 t
ORIGIN      
        1 atttaaggag aattaacatc attatgaaaa agaaattagc aacaacagtt ttagcattaa
       61 gttttttaac ggcaggaatc agtacacacc atcattcagc gaaagctttt acttttgaac
      121 cgtttcctac aaatgaagaa atagaatcaa ataagaaaat gttagagaaa gaaaaagctt
      181 ataaagaatc atttaaaaat agtggtcttc ctacaacgct aggaaaatta gatgaacgtt
      241 tgagaaatta tttaaagaaa ggcacaaaaa attctgctca atttgaaaaa atggttattt
      301 taactgaaaa taaaggttac tatacagtat atctgaatac accacttgct gaagatagaa
      361 aaaatgttga gttactaggt aaaatgtata aaacatactt ctttaaaaaa ggagagtcta
      421 aatcatctta tgtaattaat ggtcctggaa aaactaatga atatgcatac taatagtagt
      481 tacataaatt aaaaggtaga tatttctttt ttatataaag gtttggcaga catttcataa
      541 cttgccaaac ctttatatat ctaattatca aactgcacta aacttaccaa aactgcttat
      601 tctattacct gccttgtcta cctctcctgt cgctatataa cgacgttgtc cactattagc
      661 aatataagta atccatctat agccattaat gcaatatgcg ccgtcatatt tgatagttgc
      721 gttattgggt aatacacctg taattcttga attagttgaa tagccatccc tcacgttatt
      781 acctttaaca ttggcaactg tgtaataacc agtctctttt ttatacggta cattgttttt
      841 atcgagtgta taacctgctg gcactggtga attcttttca tttttagctg gtgttttaac
      901 attactgata cctgatacac acttccaata aaaataacca caccatttaa gatgcggtgt
      961 agcgactgta atatttctat gttgttgaga tatatgtatc gagtgaaggg caaaaaggat
     1021 atcaattgca ggataagtgt taggttacta ggccacttaa caggctatat agttcactcc
     1081 tactatatac agttaattat aacataaaaa gcaccccgta aactattata cgggaatgct
     1141 aaagtcatat atactacgag ggagtagtat gaaaactatg ctctctatca taagaaaaaa
     1201 cacccagtga catgcttggg tgaacaagga tagatgtaaa tagttgatgc atgtgtaaca
     1261 catcataaca aaaaactagc ccgaaagcta gctataacat acaatctaaa aagacgtccc
     1321 ttgaatacgt ctagaaagat tataacataa aaaaataggc aagtaccgaa gtacctgcct
     1381 aaaaaaggat tatccacttt ttcatcctaa ctgatttctc cccataagtc acctaatatc
     1441 tgattaggtg gggaagaacc attcgtgcat gaatgagaat ttgatgaaag ataattttca
     1501 ctacacatat tcaatcaaga cattgctttc tataataaac aactattttg ttttatttct
     1561 tttctataat aacctttgta attaagttga atccagggtt tttaatatgc tctgataaat
     1621 ctgggacaac aaaacctttt tctgttatag ggaaagactt cgtttcttct tttttcttat
     1681 tcttatcata ataagtgact tcgatctttg cgcttggatc taattcaact actctaaact
     1741 ctttatatgc tgtcgcatct aatgcccatt cgacatagta ttcaattttt tcttttgtaa
     1801 gtgtagtccc aggtttaata ggaaactcga cataatgagg ggatagcaat tcatttcctt
     1861 taccatcaac tccagtcaca tttaccatca aatacgggcc tgttggttca aaataactcg
     1921 cgtcatcgcc ttttttatat tttcctttgt cgaatgaact tgatgcactt acctcattag
     1981 taattgaaga aaatgagaat aataacaata aaacagttaa aaataataaa cctcttttga
     2041 gcatggcgct tcctccaaat ataatatata tgtttttatt tatctctttt tatagctaac
     2101 acccttattt aagatgataa atatcaacaa cttcatttta tattgagaaa atattaaaaa
     2161 tcaataaaat attaaaagaa actaaaatta cattaaaaaa taattaacaa atatttaaca
     2221 ttttaaccta ag
//
LOCUS       DAKTIP010000282         2230 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.287, whole genome shotgun sequence.
ACCESSION   DAKTIP010000282 DAKTIP010000000
VERSION     DAKTIP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.287"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            26..934
                     /locus_tag="O0305_002694"
     CDS             26..934
                     /locus_tag="O0305_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCY0275790.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIFIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            complement(1150..1398)
                     /locus_tag="O0305_002695"
     CDS             complement(1150..1398)
                     /locus_tag="O0305_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275791.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDGTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(1414..2169)
                     /locus_tag="O0305_002696"
     CDS             complement(1414..2169)
                     /locus_tag="O0305_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCY0275792.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGEIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
BASE COUNT          758 a          381 c          349 g          742 t
ORIGIN      
        1 aaagtgtatc gaaaggagaa cagacatgca aaaaaatata ttaaaaagtg gtatttcatt
       61 atctgaatta ggtctaggtt gcatgagttt aggtacagat ttaaaaaaag ccgaacaaat
      121 tatagattgt gctgttgaaa atggtatcac ttattttgat acagcagata tgtacgataa
      181 aggtataaat gaatcagttg ttggtaaggc acttcttaaa tatcaacaac gcgatgatat
      241 ttttattggt acaaaagtag gcaatcgttt aacaaaagat ggcagtacaa catgggatcc
      301 gagtaaatcc tatattaaag aggcagttaa aggttcacta aagcgtttag gtatcgatca
      361 tatcgattta tatcaacttc atggcggaac cattgatgac ccattagacg aaacaataag
      421 cgcatttgat gaattgaaac aagaaggaat tatacgtgct tacggtattt cttctattcg
      481 cccaaatgta attgattatt atttaaaaca tagtcaaatc gaaacgataa tgtctcaatt
      541 caatttgatt gataatcgtc cagaatcatt attagatgca attcacaaca atgatgttaa
      601 agtattggca agaggacctg tgtctaaagg attattaact tcaaacagtg ttaatgtgct
      661 cgacaataaa tttaaagatg gtatttttga ttattctcat gatgaattgg gtgaaacaat
      721 agcctctatt aaagaaattg aaagtaattt atctgcattg acatttagtt atttaacatc
      781 acatgacgtg cttggttcca tcattgtagg tgcaagtagc gtcgaccaat taaaagaaaa
      841 tattgaaaac tatcatacta aagttagttt agatcagatt aaaacagcaa gagctcgtgt
      901 aaaggatttg gaatatacca atcatttagt gtagaagtca ttttcagtaa taaaaacagc
      961 agcatgaggc gtttcattat aaaaatgcct tactgctgtt gtttatgtac aattcgctat
     1021 aatttatgat tatgattact cacttatgat agaaattaaa gcgttgtcct cacgcatcag
     1081 tatttagtaa tttcgccttg cggcattgcc ttaagcaaac ttctgccact tcatctctta
     1141 ataattttat taaaacatct ttctatattt cacttcgcat gttgattcat cattattagt
     1201 tattatttgt acacccagca catttccttg caacacaagt agtttgaatt tttcacaagt
     1261 ataatataat gtaccgtctg aaatttggtc tacagaaata tcgcctaaaa tatccagcac
     1321 tgtaaattct tcaaatactg atagttgttc cgcatatcgt acacaaagtc ttaccacact
     1381 ctccgattga cagttcattg ccatcccacc tatttatgct ttatttttaa ataatttagg
     1441 gaaacatcgt tcaaaaaatc taggcgcaat ttgatacatt ttcaacgcat gatgcatcca
     1501 tttaggccga ttaatttcca attgttttgt tttaatgcca taaatgatat cttctgcaag
     1561 ctgattagca tcaagcataa tttcccccat ctttttagca tacttcattg atgggtcggc
     1621 tttttgatga aaaggtgtat caatcgggcc aacattaact gtcatgatat gtaagtttgg
     1681 tgactctagt cttaaagcat tcattaatgc ataaaaccct gctttcgatg ccccataatg
     1741 tgcagcattt gcttgtgtgg aaaatgcagc ttgacttgaa atacctacaa tatgtgcgtt
     1801 agatgttaaa tatggtctca acacagtata taaaacatta aaactaatta aattaagctg
     1861 atacgtttca atcatttctg aaaaactatg gtctgaaata gatttgaaat aacctaaacc
     1921 tgcactataa atgaatccat cgaatgatgt attgtcttca aattgcagtg cctgtatcga
     1981 cttcaaatca tttaagtcac aaggaataac atttatagtt ttccccaatt cctgttcaaa
     2041 gattctagtt gctttatcaa catcacgcac caacaacgtt acatgcactt tattttctag
     2101 taactttcgg acaatcgata aacctaaacc actcgtacca ccagtcacta taaaatgttg
     2161 tcctttcatc aattaacctt ccttttcaat tatatagaat gcaatttatc aactttacat
     2221 aattgagaca
//
LOCUS       DAKTIP010000283         2190 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.288, whole genome shotgun sequence.
ACCESSION   DAKTIP010000283 DAKTIP010000000
VERSION     DAKTIP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.288"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            39..899
                     /locus_tag="O0305_002697"
     CDS             39..899
                     /locus_tag="O0305_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0275793.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEMILYKYSFSTKQMIEFL
                     GIESLKKHLKNSGGIALLPEFIVADELENKSLFQIDVDVDIPNLETTLIINPESNKQV
                     LESFVKDVFL"
     gene            complement(1254..1787)
                     /locus_tag="O0305_002698"
     CDS             complement(1254..1787)
                     /locus_tag="O0305_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCY0275794.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTTNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVLYISQ"
     gene            1878..>2190
                     /locus_tag="O0305_002699"
     CDS             1878..>2190
                     /locus_tag="O0305_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0275795.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKNNTMSILCSELLFNYL
                     SE"
BASE COUNT          857 a          263 c          322 g          748 t
ORIGIN      
        1 aggattcgat aaaagggaac cctaaaggag gtactacttt gaacttaaac attttaaaat
       61 catttttagt aactagtgaa acaaagaacc ttactaaagc atcagagtta cttaactatt
      121 cacagtcaac tgtatctaca catattgaaa aattagaaag gcaattagat gttaaattat
      181 tttatagaaa aaaatatggt atggaactaa cggaagaagg cttagcatac gttaaatacg
      241 ctaaagtgat tttagatagt aatagcgaat acgagagaga aataaaagga ctttacaata
      301 agaaggtaaa tataagtatt aacatgcaag aaagtcagta tttgtatcgc tactataata
      361 agattagtga atggttagct gaacacccat atgtaaactt gaagtttaaa tccgcacatt
      421 ctaatttcta tattaaagaa gaaattgcta atttcaaatc ggatattagc cttatcacag
      481 acgaaaagat tattaatagt aacttaactg ctattcctat aactgaagaa cgtttaatat
      541 ttgttactaa caaagagatg aaagatttca aattaaacga tattcaaaac catacattac
      601 ttgttactga aaaagggtgt agttacagag aacagttaga aatgatttta tataaatata
      661 gcttttcaac aaaacaaatg attgaatttc taggaattga gtcattaaaa aaacacttga
      721 aaaattcagg tgggattgcg ctattgccgg aatttattgt tgcagatgaa ttagaaaata
      781 aaagtttgtt tcaaatagac gtagatgtcg atattccgaa tttagaaaca acattgataa
      841 ttaatcctga atcgaataag caagtacttg aatcttttgt aaaagatgtt tttttataat
      901 tattggtgaa aacgtgtagt tatggtgaaa ctcaaagata ataatttaaa tgagatgtta
      961 atgaaaaagt aattcaatat aaaaacaggt gatttatatc tttaataagg attatttcta
     1021 gttgaaattt caaattgcgg gcattcattt tcaaaatagt aaagaattta atagatgaaa
     1081 acctttgaat catactagtt agtatggatt gattaatatt ttaacctgct aaatgagtag
     1141 cttatgtatt ttaaatagct gtcatctagc aggttcttta ttattaataa gttcagataa
     1201 attattttag tctgttttct aatgttgata ataataactt taaatctgta ctttcattgt
     1261 gagatatata gtacatcata aatttttata tttggattga gactctttgg aagattttca
     1321 ataaatgaat atttcacaaa cttccactta tcaggattat aaattataat ttctgcaact
     1381 gcattatcga agattctaaa caattttttg atttctattt cttttagaga taaagtaggg
     1441 aaaatgtcga tatattcttc aagcgcataa agactatgtt taacagaatc ggttatgttc
     1501 attgaatata tcacaccaat atttatattt tgccatccaa aagaagaaat aacattatca
     1561 taaaatccat tgaatataaa ttcagtcatc ccctctgaac ttctccataa atataatggt
     1621 gaataagtat ttgtaatgca attagtagtt gtttgactta ttaaataagc ttttataatt
     1681 aaatttttaa atccatcagt cttataacca tttaatcgaa ctctatctct aattatttcc
     1741 atattgtaat cactaggcaa cttaacatga tattgcattg catgcattta gcataccccc
     1801 ttttataaaa aggatagcaa taataagtaa aatctcatat tatccaattg tgatatagtt
     1861 atcataaaaa gtgataggtg attaaattga actttaatga tttggaaatt tttataactg
     1921 tatgtgaaga agcatctatc aataaagctg caattaaact tagatatgca caatctaata
     1981 tatctcaaag aattagcaag cttgaaaatg aattaggtgt agttttgctt tttagaaatc
     2041 aaaaaggtgc taaggcaact aaagcaggcg aagaattctt agcgtatagc aaaaaagtat
     2101 taagagatac agagactata aaaaataaaa tgaaaaataa tactatgtct attttatgct
     2161 cagaactgtt atttaattat ttatctgaga
//
LOCUS       DAKTIP010000284         2190 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.289, whole genome shotgun sequence.
ACCESSION   DAKTIP010000284 DAKTIP010000000
VERSION     DAKTIP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.289"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            256..777
                     /locus_tag="O0305_002700"
     CDS             256..777
                     /locus_tag="O0305_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCY0275796.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            complement(947..2113)
                     /locus_tag="O0305_002701"
     CDS             complement(947..2113)
                     /locus_tag="O0305_002701"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCY0275797.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
BASE COUNT          694 a          364 c          414 g          718 t
ORIGIN      
        1 tttaataagt aattataggt ttaataaaat attaaggatt cattgcattt tcaatatttt
       61 aatttaagat ttggtttaca aagtaagagg ttagcacgtg tagtaagata tgtaactgta
      121 tattgcagca aagtgatagc tggtattacg tgtttatata cgtaattgac gacttattta
      181 tttttaaaaa atgtgaacat gaatattttc taatttaaca attaaggcgt atgattagaa
      241 aaagggggga tttttatgga gtataagagt tactatgatt cgcctgtagg acgattggaa
      301 cttttgagtg atggggtaag tctgaccgct gttttgtttg aaaatcaaca aggtgatggt
      361 actagggaag aaaatacgtc tttagcgata tttagagagg cgacacagtg gttagacgcg
      421 tattttaaag gtgacaatcc tgaaatcaca atacctttaa agccaacagg tagtcatttt
      481 caacagtgtg tttggaatga attaagacag gttccttatg ggactctaac aacgtatggt
      541 gctattgcca aaaaagtagg caagctgctt aacaagccaa aaatgtcagc ccaagcggtt
      601 ggaggtgcag tcggaagtaa tccattatct attatcgtgc catgtcatcg tgttgtcggt
      661 aaaacgggta gcttaacagg gttcggtgga acgattaata ataaaattaa gctgttagag
      721 ttggagaata tcgatatgtc aaagctttat gtaccaaaac atagcacgaa gccgtaacgt
      781 ataaaggatc caggcattag gaaaacttca ataatgtgtc aaataggcat aatgaattca
      841 ttcaatacga agtgttataa atagagaaca gcagtaagac gatttcaatt agaagtggtc
      901 ttactgctgc ttttaggttt tattatatgt gttttaaata caccgactac tctggtctgt
      961 gatattcacg aacgttattt tcaatatttg ataagtagaa aatatgacga tcttcatgaa
     1021 cagcatcttg ttcttcgtca aattcaacgt catcaaaacg tttgaagaat gtttcatatg
     1081 catcaacaga tacttcagta cggttattta ataatgcttt atgtgcagct tgatctaaat
     1141 gatctttgta gccttcaact aatgtcgcac tataaaattc accaactgaa cctgagccat
     1201 aactgaataa accgattgtt tcaccagctt gtaaatctcg attttcaagt aatgatatta
     1261 ggcttaaata taatgatcca gtataaatat taccgacata acggttataa tctacagcat
     1321 cttcatatcc tgaacgtaaa cgctcttgag ttgtttcatc agcgttatca atgattgact
     1381 ctaatgcctt tttacccatt tttgtaaatg gaacatggaa gcatagagat gcgaagtcag
     1441 ctagcgactt accttgacgt tttgcgtatt cattccagct ttgttggaat gagcggatat
     1501 aagcatcttt agataatgca ccatcaacta atggatattt atgtccagtt ggacgccaga
     1561 aatcataaac gtcttcagtg taagcaacag catcttcatt taatgccaaa atgcttggat
     1621 tatgtgcaat aaccatcgca actgcgccag caccttgtgt tggctcgccg cctgaattca
     1681 atccataacg tgctgtatct gtagcaataa ctaatacttt ttcattcggt ctagttgcta
     1741 aataatcttt agctaattga attgctggtg ttgcagcata acaagcttct ttcatttcaa
     1801 agcagcgtgc aaaaggttga atacctaata agttgtgaat ttgaacagcg gctgctttag
     1861 cagcatcaac tgctgattca gttgccacaa ttaccatacc aattttcttt ttgtcttcgt
     1921 ctgttataat gtccttagca gcgttagcgc ccattgaaac gatgtcttgg tttacaggac
     1981 taacagccat ttcagtttga ccaattccaa ttaaaaattt gtttgggtct acttggcgtg
     2041 cttctgctaa tttagccatg tctacatagt actttggaac gtaaaagttt attttatcga
     2101 tacctattgt cataaggttt tacatccttt caaatttata aaagcaacac ttgtatcata
     2161 tcagaatatg ctaattttat acaaagaaat
//
LOCUS       DAKTIP010000285         2189 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.290, whole genome shotgun sequence.
ACCESSION   DAKTIP010000285 DAKTIP010000000
VERSION     DAKTIP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.290"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(136..705)
                     /locus_tag="O0305_002702"
     CDS             complement(136..705)
                     /locus_tag="O0305_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0275798.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
     gene            915..1133
                     /locus_tag="O0305_002703"
     CDS             915..1133
                     /locus_tag="O0305_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCY0275799.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(1214..>2189)
                     /locus_tag="O0305_002704"
     CDS             complement(1214..>2189)
                     /locus_tag="O0305_002704"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCY0275800.1"
                     /translation="SNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQ
                     NTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPI
                     VQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALK
                     VNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDW
                     ENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFF
                     GVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
BASE COUNT          743 a          339 c          299 g          808 t
ORIGIN      
        1 tgtatgtgtt tgaataacaa gcacaaatat tattataaca agattaagtt aatttgcaat
       61 agatacctta aaaaaatatg cagaacattt acactatatt tataaaacat tccgcatatt
      121 ttaaaaataa acaatttatt tatcttcaaa taaagaatag cgtgtcaatg cttcaaacac
      181 attcaatgtg gctttattat aatctattgg ttggtcggga ttttttgata tcatgcgaga
      241 ttgtttatct accatgtaat agtaaaaaat ggaattgtta tattgatgcc ataaactgtg
      301 gtatgaaaca tgaagaatga ttgattcatt attaataaat gtcactttac atttacgatt
      361 ttttaattct ttaatacttt cgatataatg catatttaac caaatatttt cattttgtct
      421 gtcagaatgt gttgggaaaa aataagttgg aaataatggt gttagtaaaa taggtggttt
      481 actagaaatg ccagtaatgc ggtttgtctc tgctttcttg ccaagataag tattaccata
      541 aaatttgcaa gagcgttcaa tgattttctg gactttaaaa ggattttgaa tacgcgtttt
      601 gtcaaaccga attatttcac taccgtttct ttgatcgtca tcatcaaatg ctggtcgaat
      661 aaccatgtct cctttgcgta tcacataaat attttgagaa tacataagca aaacctcgct
      721 ttattaagtt ttaaaccatt aatataaaaa tacaattaat gatataaaga tattttataa
      781 taaaattctg agttgtcaag ttattatttt ggtgaaaatg tacaataatt tttaactaat
      841 agtcagaata ttcattgatt tcaaaattag aaaaatgtat acttattata aattaaagaa
      901 aggtagtggt ttctatgaaa tacaatacta atgttaaaca tacaacttta gaagcgtttg
      961 tcacaactgt caatgatttg ggtattgaat taattatcaa tgaagcactt cgagaggtaa
     1021 gaaaacgaca gctcatagaa cttatagatg acgcactcgt caataaagat gaagcagcat
     1081 ttaatcaata tacggcagaa tacaaaaatt tggaggcatt tctcggtgaa taacattgat
     1141 tcgaattcca taacgcaatc ataaaaggac tattcttaac atctcataca tgtaagaata
     1201 gtcctttaat cgtttatgac attaatctaa ttaattcatg tctatctatg tcaccgaaat
     1261 aatgatataa atcatattct gatagtgctt cttctatgtg ctcaaagtcg tgtagacagc
     1321 caactaatgc attttcaaga tcagtgacat ctccgacacc aaagaaatca ccgaatattt
     1381 ttgcatgttc gattttacct cgtttaacat caaacttaat ttgtacaaat cctttttcaa
     1441 atttttcctc gcgttcaaag ttatatttag ggtttctacc ataattccat tcccaagttc
     1501 tatatttgtc gttacttaac ttttcaatat tttcccaatc ttcatccgtt aatttatatt
     1561 cttctacttc agtttctcca aagatagttt tcaagatgat ttttttaaat tcttcaattt
     1621 ctaaagggtc atttaaaaat tcttgaatgt ttgctacccg tttacgaaca gatttaatac
     1681 cttttgattt aatcttagct ggattaactt ttagtgcatt ctgaacttcg tctaaatcac
     1741 tatttaacat caacgtacca tgactaaaca ttctattttt aactttaacc atagcattcc
     1801 ctgagatttt cgcttgtcca acttgaatat cgttacgacc agttaattca gcatttacgc
     1861 ctaaagattg taatgcttga acaattggtt cagtgaattt ttggaaattg tggaaactgt
     1921 taccatcatc atctgttata aaactaaagt ttaaattgcc agtatcatga taaacagcgc
     1981 caccaccaga aattcttctt actacatcga tgttgtgagc atcgatatat gtctgattta
     2041 cttcctctat cgtattttga ttctttccaa caatgataga tggtctattt atgtaaaata
     2101 aaaagtaact ttcttctgct ggtaaatttt ttaaaacata ttcttccatt gctaagttta
     2161 aagttggatc tgtaatatta ttattacta
//
LOCUS       DAKTIP010000286         2181 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.291, whole genome shotgun sequence.
ACCESSION   DAKTIP010000286 DAKTIP010000000
VERSION     DAKTIP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.291"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            103..1380
                     /locus_tag="O0305_002705"
     CDS             103..1380
                     /locus_tag="O0305_002705"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCY0275801.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKKEPRANTQAAEHILQEIRQQ"
     gene            1959..>2181
                     /locus_tag="O0305_002706"
     CDS             1959..>2181
                     /locus_tag="O0305_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275802.1"
                     /translation="MEIIIEIYMYLRDGIKEHFILKKVRKKFKKLNEINSDIQNMYAE
                     NQGLLETDKTIIDKVLSTDVTNSHDINELY"
BASE COUNT          774 a          308 c          430 g          669 t
ORIGIN      
        1 gacaattatt caattgaatt ttaagacgaa tgacgttatt ttgtggtaaa aatagtagtg
       61 attgaaatgt taaaaattat gtgaagtaaa aggagtacgt ccatgcaaaa tttagataag
      121 aattttcgac atttatctcg taaagaaaag ttacaacaat tggttgataa gcaatggtta
      181 tcagaagaac aattcgacat tttactgaat catccattaa tcgatgaaga agtagccaat
      241 agtttaattg aaaatgtcat cgcgcaaggt gcattacccg ttggattatt accgaatatc
      301 attgtggacg ataaggcata tgttgtacct atgatggtgg aagagccttc agttgtcgct
      361 gcagctagtt atggtgcaaa gctagtgaat cagactggcg gatttaaaac ggtatcttct
      421 gaacgtatta tgataggtca aatcgtcttt gatggcgttg acgatactga aaaattatca
      481 gcagacatta aagctttaga aaagcaaatt cataaaattg cggatgaggc atatccttct
      541 attaaagcgc gtggtggtgg ttaccaacgt atagcgattg atacatttcc tgagcaacag
      601 ttactatctt taaaagtatt tgttgatacg aaagatgcta tgggcgctaa tatgcttaat
      661 acgattttag aggccataac tgcattttta aaaaatgaat ttccgcaaag cgacatttta
      721 atgagtattt tatccaatca tgcaacagcg tccgttgtta aagttcaagg cgaaattgat
      781 gttaaagatt tagcaagggg cgagagaact ggagaagagg ttgccaaacg aatggaacgt
      841 gcttctgtat tggcccaagt agatattcat cgtgcagcaa cacataataa aggtgttatg
      901 aatggcatac atgctgttgt tttagcaaca ggaaatgata cgcgtggtgc agaagcaagt
      961 gcgcatgcat acgcgagtcg tgacggacag tatcgtggta ttgctacatg gcgttacgat
     1021 caagatcgtc aacgattgat tggtacaatt gaagtgccta tgacattggc aatcgttggc
     1081 ggtggtacaa aagtattacc aattgctaaa gcttcattag agctactaaa tgtagagtca
     1141 gcacaagaat taggtcatgt agttgctgcc gttggtttag cgcaaaactt tgcagcatgt
     1201 cgcgcgcttg tgtcagaagg tattcaacaa ggtcatatga gtttacaata taaatcatta
     1261 gctatcgttg taggggcaaa aggtgatgaa attgctaaag tagctgaagc tttgaaaaaa
     1321 gaaccccgtg caaatacaca agcagcggaa catattttac aagaaattag acaacaatag
     1381 taaatggcga atagcgattt taaagaaaag tttaagtcag tccatcgtag cgcttagata
     1441 aattaatatg aacaaaaata aaaacttcta tcacacgcat acaggaaatt gaagcgttcg
     1501 tgatagaagt tttttgatta gtaaatcagt ctaatgaaaa gtaaataact tcttcatgag
     1561 acgaattcat tataggattc aaatttggaa cgagtttctt attgtctcga ttttaacagt
     1621 cttagattct tcaagttcct atcattttga tatttgttaa caatatgaac agtagaatgc
     1681 ctcactccac tcctttttta gcaatttctt ctgcagtcat catttattca tcatataaga
     1741 atttaatttc tcatttctta tgacttgctt gtaagtatga aagaacaaca gcattgcatt
     1801 taaatacaat tattattaat tattccacta gcgttaaaaa gaagtaataa taagatactt
     1861 aagaataaat atctgaaaat actaaaaatt ctgaaaaaac ctttcttatt tttaatatat
     1921 tgagtataat gttttttgag agaggagata tgacatttat ggagattata atagagattt
     1981 atatgtattt aagagatgga attaaagagc attttatttt gaaaaaggtt agaaaaaaat
     2041 ttaaaaaatt aaatgagatt aacagcgata tacaaaatat gtatgcagaa aatcaggggc
     2101 tcttagaaac agataaaaca ataattgaca aggtcttaag cactgacgtt acaaatagcc
     2161 atgatattaa tgaattatat t
//
LOCUS       DAKTIP010000287         2160 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.292, whole genome shotgun sequence.
ACCESSION   DAKTIP010000287 DAKTIP010000000
VERSION     DAKTIP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.292"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..889
                     /locus_tag="O0305_002707"
     CDS             <1..889
                     /locus_tag="O0305_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0275803.1"
                     /translation="RSYSLYLWHYPIIVFVNSYYVQGQIPVYVYIIEILLTALMAEIS
                     YRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSIVVLSGQFDALGKQHEAEK
                     KEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLIGDSVMVDIGNVFTKKIPN
                     AQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNGAFTKDQLNELLDSFGKAD
                     IYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASAGHPEYFAYDGIHLEYAGS
                     KALTDLIVKTMETHATNKK"
     gene            1250..2017
                     /locus_tag="O0305_002708"
     CDS             1250..2017
                     /locus_tag="O0305_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0275804.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
BASE COUNT          788 a          326 c          390 g          656 t
ORIGIN      
        1 acgatcatat agcttatact tatggcatta tcctatcatt gtttttgtga acagttatta
       61 cgtacaagga caaataccgg tatacgttta tattatagaa attttgttaa cagcgttaat
      121 ggctgaaatt tcgtatcgct ttattgaaac acctatacgt aaaaaaggat ttaaagcttt
      181 tgcattttta cctaaaaaga aggggcaatt tgctagaaca gtgttagtta tcctattatt
      241 ggttccgtct atcgttgtgc tcagtggaca gtttgatgca cttggcaaac aacatgaagc
      301 cgagaagaaa gagaagaaga cggaatttaa aacaacgaag aaaaaagtcg ttaaaaaaga
      361 taagcaagag gataagcaga cagcgaatag caaagaggat attaaaaagt catcaccact
      421 attaattggt gactcggtca tggtggatat tggtaatgtc tttactaaga aaataccaaa
      481 tgcacaaatt gatggtaaag ttggacggca actcgttgat gctacaccaa ttgtgaaatc
      541 gcaatataaa gactatgcta aaaaaggtca aaaagttgta gtagagcttg gtacaaatgg
      601 ggcatttacg aaagatcaat taaatgaact attggatagt tttggaaaag cagacatata
      661 tttagtttct attagagtac ctagagatta tgaaggtaga ataaataaat taatttatga
      721 ggcagctgaa aagcgctcta atgtacatct agtcgattgg tataaagctt ctgcaggtca
      781 tccggaatac tttgcatatg acggtattca cttagaatat gcaggtagta aagcgctgac
      841 tgatttgatt gtaaaaacga tggaaacaca tgctacaaat aagaaataat ttgatgcact
      901 aaacttttga aatattacat tacttctgat atttattatc aaaaatgatg tatttcatta
      961 aaagtttagt gcttttttat tttcaaatcc catagtaacg gtgcagaaaa agtgttgtaa
     1021 acattctaat tggtatatta cattcaatga agctttatta ggaacagatt acattatgat
     1081 aacaaagccc gcaagacacc taatctctgt tatagtttgt tttgtcgcaa aactataaaa
     1141 gttataattg tttgcatact aaaaaaataa aaaatataaa atttaaaata attgagtcgc
     1201 taatgactat atgtatagag tgttttgatt attgggagga tatttaatta tgaaaaaaat
     1261 cgttacagct acaatcgcta cagcaggact tgccactatc gcatttgcag gacatgatgc
     1321 acaagccgca gaacaaaata acaatggata taattctaat gacgctcaat catacagcta
     1381 tacgtataca attgatgcac aaggtaatta tcattacact tggacaggaa attggaatcc
     1441 aagtcaatta acgcaaaaca acacatacta ctacaacaac tacaatactt atagttataa
     1501 caatgcatct tacaataact actataatca ttcatatcaa tacaataact atacaaacaa
     1561 tagtcaaaca gcaacaaata actattatac tggtggttca ggtgcaagtt atagcacaac
     1621 aagtaataat gttcatgtga ctacaactgc agcgccatct tcaaatggtc gttcaatttc
     1681 taatggttat gcatcaggaa gtaacttata tacttcagga caatgtactt attatgtatt
     1741 tgatcgtgtt ggtgggaaaa ttggttcaac atggggtaac gcaagtaatt gggctaacgc
     1801 agctgcatca tctggctata cagtgaacaa tacaccaaaa gttggtgcta tcatgcaaac
     1861 aacacaaggc tattacggtc atgttgctta cgttgaaggc gttaacagca acggttctgt
     1921 tcgtgtttca gaaatgaact atggacatgg tgctggtgtg gttacgtctc gtacaatttc
     1981 agcaaaccaa gcaggttcat ataatttcat tcattaatca aatgtaaatc aaatgacgtc
     2041 aatattctct aacatgagag tattggcgtt tttgttttat ataaatataa atgagagcgg
     2101 tttattcact gatctttagg gaactaagta ataaagtgat aatttatact atgtcagtat
//
LOCUS       DAKTIP010000288         2102 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.293, whole genome shotgun sequence.
ACCESSION   DAKTIP010000288 DAKTIP010000000
VERSION     DAKTIP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.293"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..896
                     /gene="hlgC"
                     /locus_tag="O0305_002709"
     CDS             <1..896
                     /gene="hlgC"
                     /locus_tag="O0305_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCY0275805.1"
                     /translation="LANPLLENAKAANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNI
                     QFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYV
                     SLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVE
                     QQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSG
                     FNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNR
                     NYTVKYEVNWKTHEIKVKGQN"
     gene            898..1875
                     /gene="hlgB"
                     /locus_tag="O0305_002710"
     CDS             898..1875
                     /gene="hlgB"
                     /locus_tag="O0305_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCY0275806.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(1927..>2102)
                     /locus_tag="O0305_002711"
     CDS             complement(1927..>2102)
                     /locus_tag="O0305_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCY0275807.1"
                     /translation="VLASVLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPL
                     YLTLRKRMAVLLR"
BASE COUNT          820 a          340 c          346 g          596 t
ORIGIN      
        1 ctcttgccaa tccgttatta gaaaatgcta aagctgctaa cgatactgaa gacatcggta
       61 aaggaagcga tatagaaatt atcaaaagga cagaagataa aacaagtaat aaatggggcg
      121 tgactcaaaa tattcaattt gattttgtaa aggataaaaa atataacaaa gatgctttga
      181 tattaaagat gcaaggattc attagctcta gaacaacata ttacaactat aaaaaaacta
      241 atcatgttaa agctatgcga tggccattcc aatataatat tggtttaaaa acaaatgata
      301 aatatgtttc tttaattaat tatttaccta aaaataaaat tgaatctaca aacgtgagtc
      361 agacattagg atacaatatc ggtggtaatt tccaatcagc cccatcactc ggtggtaatg
      421 gatcatttaa ctattctaaa tcgattagct atacacaaca aaattatgta agtgaagtag
      481 aacaacaaaa ctcaaaaagt gttttatggg gcgtcaaagc gaattcattc gccactgaat
      541 caggtcaaaa atcagccttt gatagcgatt tatttgtagg ctacaaacct catagtaaag
      601 atcctagaga ttatttcgtt ccagacagtg agttaccacc tcttgtacaa agtggattta
      661 acccttcatt tatcgccaca gtatctcatg aaaaaggttc aagcgataca agcgaatttg
      721 aaattactta cggaagaaac atggatgtca ctcatgccat taaaagatca acgcattatg
      781 gcaacagtta tttagacgga catagagtcc ataatgcatt tgtaaataga aactatactg
      841 tgaaatacga ggtcaattgg aagactcatg aaatcaaggt gaaaggacag aattgatatg
      901 aaaatgaata aattagtcaa atcatccgtt gctacatcta tggcattatt attactttct
      961 ggtactgcta atgctgaagg taaaataaca ccagtcagcg taaaaaaagt cgatgacaaa
     1021 gttactttat acaaaacaac agccacagca gattctgata aatttaaaat ttcacagatt
     1081 ttaacattta atttcatcaa agataaaagt tatgataaag atactttagt acttaaagct
     1141 actgggaata ttaactcagg ctttgtgaaa cctaatccta atgactatga cttttcaaaa
     1201 ttatattggg gagctaaata caatgtatct ataagctcac aatctaatga ttcagtaaac
     1261 gtcgttgatt atgcaccaaa aaatcaaaat gaagagtttc aagttcaaaa tactttaggc
     1321 tatacatttg gtggtgacat tagtatctct aatggtttat ctggtggact taatggaaat
     1381 acagcttttt ctgaaacaat taattataaa caagaaagtt acagaacaac attaagtcgc
     1441 aacacaaatt ataaaaatgt tggctgggga gttgaagcac ataaaattat gaataatggt
     1501 tggggacctt atggaagaga tagcttccac ccaacatatg gtaatgaact cttcttagct
     1561 ggcagacaaa gcagtgcata cgctggccaa aacttcatag cgcaacacca aatgccatta
     1621 ttatctagaa gtaacttcaa tccagaattt ttaagcgtac tatcacacag acaagatggc
     1681 gctaaaaaat ctaaaattac agtaacttat caacgtgaaa tggatttata ccaaattcgt
     1741 tggaatggct tctactgggc aggcgcaaat tataaaaact ttaaaactag aacatttaaa
     1801 tcaacatatg aaattgattg ggaaaatcac aaagtgaaat tgttagatac aaaagaaact
     1861 gaaaacaata aatagctagt aaaacacggt cgccaacagt aattgtgacg accgtgtttt
     1921 gatttattat cttagtaata ctgccattct ttttctcaat gtgagatata aaggaatagc
     1981 tacaattaaa gtgaatatta cgcctggaat cgcgtttaac aacactaccc acacaggtaa
     2041 atttaaaata atagataata gtagcctaga taccaaactg cctaatacac ttgctaaaac
     2101 ta
//
LOCUS       DAKTIP010000289         2101 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.294, whole genome shotgun sequence.
ACCESSION   DAKTIP010000289 DAKTIP010000000
VERSION     DAKTIP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.294"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(311..1318)
                     /locus_tag="O0305_002712"
     CDS             complement(311..1318)
                     /locus_tag="O0305_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCY0275808.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1334..1951)
                     /locus_tag="O0305_002713"
     CDS             complement(1334..1951)
                     /locus_tag="O0305_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCY0275809.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          723 a          369 c          293 g          716 t
ORIGIN      
        1 catatacaaa tttatattaa ataaagccca atacaattta ggttaattaa acaagttgat
       61 aactatttaa ttattccttc attgaagaat ataaactatt aaatcattat tttgctctta
      121 catatatttt aatgacctaa ctgattatgt tccatggaat acatttataa tatagcctcc
      181 taattaaaat gctttgtctt ggtcattcta cgtaaattct ataaaatatg ttatctactt
      241 acataaaaaa ctgaacttca ataccaccat atgtttgtga tactgaagtt cagtttagtt
      301 ttattttcaa ttagaaaaat aagttaagta tatagaatag taaacctgct aacaatgctg
      361 aaataggtaa tgtaatcacc catgtaatga tcattcgttg cgcagtgctc cattttacac
      421 ctttagctcg gttagaagca ccaacaccta agattgatga tgacacaacg tgagttgttg
      481 ataatgggaa atgtagcgat gatgcaacaa aaattgttaa tgcagatgat aaatcggccg
      541 cagcaccatt tgctggacgt attttcataa tattaccacc tacagttttg ataattttcc
      601 agccaccaat tgcagtacca agccccattg ctgtcgcaca ggcaaatttt acccataact
      661 gtggttcaac actgccatca ttctgtacat tagcgacaat caatgccaac gtaataatac
      721 ccattgattt ttgcgcatca ttcgtaccgt gagagaatga ttgtaacgct gctgtgaaaa
      781 tttggaaaaa tctaaagtta cgattcgctc ttgttaaatt tgcattttta aagataactt
      841 taaaaattga atacatcaag aaaccaacac aaaatgcgat aatcggtgaa acgattaata
      901 caataataat ttttgtgaaa ccttggtaat gtaacactcc aaatgagcct tcagatgcga
      961 ttgctgcacc cgcaattgaa cctataagtg catgtgaaga cgaacttgga attccgtaaa
     1021 accaagtagc taaattccaa ataatagccg caagtattgc agctaacaca acaactaatc
     1081 cattttccaa tttaaatgga tcgacaatgt ctttagtaat ggtgcctgca acgcccgtaa
     1141 atgttaaagc acctataaag ttcatcactg ctgccattaa aattgccgtt ttaggcgtta
     1201 acgctctagt agatacagca gtagctactg cattggctgt atcatggaat ccattgataa
     1261 agtcaaatat cagcgagaaa ataactacag ctatagtgac gatgattata tatgacataa
     1321 atatatactc cccttagcta tttttcataa taatagtttc aaaattattt gctacgattt
     1381 gacatttatc agcgatttct tccatgcttt cataaatatc ttttatttta attaaagtga
     1441 ttggatctgt ttcgctattg aaaatatgtt taattgactg tcttaaaata ccatcacagt
     1501 ttgtttcaaa ttctttaata ttaattgaat gaatacgcat atgtgataat tttttatcga
     1561 ctaataagcc gacagcaagt ttcatttctg caactgcttt ttgaatgtta tcaacaaact
     1621 cagccatata ttcatctgtg tattcgattg aatacatttc aaacatagct gccgtttctt
     1681 caattgcatc taaaacatca tcaattgcat cacataatga taaaatatct tcacgttcaa
     1741 ttggtgtgat aaatgtttga tttaaatcag taattacttg atgtactaat tcgtcaccat
     1801 gtgactcata agttttaatg ttgtctgagt atgcttttaa atctaaatgt gtattgaaat
     1861 ccattttacc gaattcaata gcagcacgat ccagattgaa aaccatctct tctaattgaa
     1921 ccataaactt atcttttttc ttactaaaca tttaaaatcc tccatttaag cgattgtcac
     1981 caatcacatt cagttataat ttgtttcaaa ttaagacaag tgaatttaca aactaatgat
     2041 acaaatttgt tattatcaat cgtcagtata attttagtgt actgatatta atttcaaaaa
     2101 t
//
LOCUS       DAKTIP010000290         2088 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.295, whole genome shotgun sequence.
ACCESSION   DAKTIP010000290 DAKTIP010000000
VERSION     DAKTIP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.295"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            316..975
                     /gene="arlR"
                     /locus_tag="O0305_002714"
     CDS             316..975
                     /gene="arlR"
                     /locus_tag="O0305_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCY0275810.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            972..>2088
                     /gene="arlS"
                     /locus_tag="O0305_002715"
     CDS             972..>2088
                     /gene="arlS"
                     /locus_tag="O0305_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCY0275811.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVK"
BASE COUNT          817 a          270 c          345 g          656 t
ORIGIN      
        1 gataataact attcatttta caatagtgaa aagtcagtat atgacaacaa ttaatattgc
       61 ggtaaggcct tgtgttacag tattctatat ttaagtactg caatcagaat taacagaatg
      121 ccattaactg attattaaat atttgagtta ataaataatt aatgattgta gcttgaaaaa
      181 tttaaaacat ggttattgat ttgtgataaa atttaaacgt aaacaaacta atttaaaaag
      241 caactattgt atagaaaaat acaaaattta aaatatatta ccttattaga aaaagtcgta
      301 atatgaggtg tacaaatgac gcaaatttta atagtagaag atgaacaaaa cttagcaaga
      361 tttcttgaat tggaactcac acatgaaaat tacaatgtgg acacagagta tgatggacaa
      421 gacggtttag ataaagcgct tagccattac tatgatttaa tcatattaga tttaatgttg
      481 ccgtcaatta atggcttaga aatttgtcgc aaaattagac aacaacaatc tacacctatc
      541 attataatta cagcgaaaag tgatacgtat gacaaagttg ctgggcttga ttacggtgca
      601 gacgattata tagttaagcc gtttgatatt gaagaacttt tagcaagaat tcgtgcaatt
      661 ttacgtcgtc agccacaaaa ggatattatc gatgtcaacg gtattacaat tgataagaac
      721 gcttttaaag tgacggtaaa tggcgcagaa attgaattaa caaaaacaga gtatgattta
      781 ctatatcttc tagctgaaaa taaaaaccat gttatgcaac gggaacaaat tttaaatcat
      841 gtatggggtt ataatagtga agtagaaaca aatgtcgtag atgtttatat aagatattta
      901 cgaaacaagt taaaaccata cgatcgtgac aaaatgattg aaacagttcg tggcgttggg
      961 tatgtgatac gatgacaaaa cgtaaattgc gcaataactg gattattgtt accacgatga
     1021 ttacgtttgt cacgatattt ttgttttgtt taattattat ttttttcttg aaagatacac
     1081 tgcataatag tgagcttgat gatgcagaac gaagctcaag cgatattaat aatttatttc
     1141 attctaagcc tgttaaagat atatctgcat tagacttgaa tgcatcttta ggtaattttc
     1201 aagagataat tatttatgat gagcataata ataaattatt tgagacatcg aatgataaca
     1261 cagtgagagt tgaaccaggt tatgaacacc gttattttga ccgcgtaata aaaaaacgct
     1321 ataaaggcat tgaatattta attattaaag aaccaattac aacgcaagat ttcaaagggt
     1381 atagcttgtt aattcattca ctagaaaatt atgataacat cgtaaaatca ttgtatatca
     1441 ttgcgctggc atttggagtg attgcaacaa ttataactgc cacaatcagt tatgtatttt
     1501 caacacaaat tactaaaccg cttgtcagtt tatcaaataa aatgattgag attcgacgag
     1561 atggttttca aaataaattg caattaaata caaattatga agaaatagat aatttagcaa
     1621 atacgtttaa tgagatgatg agccaaattg aagaatcatt taatcaacaa agacaatttg
     1681 ttgaagatgc gtcacatgaa ttacgaacac cattacaaat tattcaaggt catttaaatt
     1741 tgattcagcg atggggaaaa aaagacccag cagtattaga agaatcgtta aatatttcta
     1801 ttgaagaaat gaatcgtatc ataaaattag tcgaagaatt acttgaattg actaaaggag
     1861 atgtaaatga catttcttct gaagcacaga ccgtgcatat taatgatgaa attcgctcgc
     1921 gaatacactc attaaaacaa ttgcatcctg attatcaatt tgatacggat ctgacatcta
     1981 aaaatctaga aattaaaatg aaacctcatc aattcgaaca attattttta atctttattg
     2041 ataatgcaat caaatatgat gtgaagaata agaaaattaa agttaaga
//
LOCUS       DAKTIP010000291         2080 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.296, whole genome shotgun sequence.
ACCESSION   DAKTIP010000291 DAKTIP010000000
VERSION     DAKTIP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.296"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            121..324
                     /locus_tag="O0305_002716"
     CDS             121..324
                     /locus_tag="O0305_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0275812.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(321..557)
                     /locus_tag="O0305_002717"
     CDS             complement(321..557)
                     /locus_tag="O0305_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275813.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            782..1363
                     /locus_tag="O0305_002718"
     CDS             782..1363
                     /locus_tag="O0305_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCY0275814.1"
                     /translation="MAKTLYIMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGIFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1436..2053)
                     /locus_tag="O0305_002719"
     CDS             complement(1436..2053)
                     /locus_tag="O0305_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCY0275815.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
BASE COUNT          759 a          322 c          321 g          678 t
ORIGIN      
        1 tagaatcaga tatcaaaaaa atgtgcaaaa tcacttttgc acatttacaa atactagttt
       61 tctgataaga ttaataataa cactttaaat agcgcttaat aaatgaaggg ggcaagtctt
      121 atgacgtttt acaatttcat catgggtttt caaaatgata acacaccatt tggtatattg
      181 gccgaacacg ttagtgaaga taaagcattc cctcgattag aagaaagaca ccaagtaatt
      241 agagcatatg tgatgtctaa ttacacagat catcaattaa ttgaaactac aaatagagct
      301 attagcttat atatggcaaa ttaatttgag tagtaccaat tatgatgtat tagtgcatcc
      361 caaatatctt ttgttttaaa gtttatttca tcatttctta tcgaaaatgg tgtaataatg
      421 tctttatcta accaagtgtt gataagttca tttggtacac catctaacaa catttcactt
      481 ttactaatta taaaacattc ccagtcaagt gaaacatttt gtggattcac ataattacat
      541 tgattatgat tatccataaa cactcactcc tttaaaattc tgtactcttc attgcgtttt
      601 accccgtcac attatctttt aaactaaaat catcattact tatgaaaaaa atgtacatca
      661 aaagcaaagg ttttcgctac cgaaaaagtt taaataatgg tttaatatat ttggtactca
      721 ttttaataaa aagagaatac attttgagct atcaatactt tttattgaag aggtgttatt
      781 gttggctaaa acgttatata taatgcgcca cggacaaact ttgtttaatt ttaagggact
      841 aattcaggga tttggagatt cgccgctaac agaacttgga attgctcaag ctcaaaaggc
      901 acgtagttat tatgaaacta aggggataaa cttcgattta tatgcatcat caacgcaaga
      961 acgcgcaagt gacacacttg aaaatgttgc acctaaccaa tcgtatcaac gttttaaggg
     1021 actgaaggaa tggcattttg gaatatttga aggtgagtca gtctatctat ttgataatct
     1081 atacaagcct gaggacttat tcggagatcg aattgttcct ttcaaaggag aggcaaggca
     1141 acaagttgaa gaacgcattg tgaaaacttt acatgacatt atgtctcaaa caaagaataa
     1201 tgcattagtc gtgagtcatg gaacaataat gggagtattt ttaagatatt gccttaaact
     1261 agatgaagca ttaaagcata atatcggtaa ttgtaatatc ctgaaatttg aatatgacaa
     1321 tggaacattt aaatttgttg agttaattga tccaaattta taataaataa agcgttatac
     1381 atagacacaa gtatgtcttt tgtataacgc ttttaaaata caactacata acgctttaga
     1441 ataataattg aataagtttt tgtaatatca tcagagcaac aataataatt attatgcttg
     1501 atattttatt gattattgtt aataattttc cggttttatc aattgatcca accatttttc
     1561 ctaaaattgc gagtaaaaag aaccataacc atgaaacgct aatacaagcg attgtaaacg
     1621 caattttatt gctgccacta tataatgcag cactactacc aattactcca attgtatcta
     1681 aaatagcatg tggattgagt aatgaaactg ataaagcaaa acttacttgt ttcattggag
     1741 acataatttg agtttctcca tctgttgagg gtttatcatg ccaaatggtc caagccatat
     1801 acatcaagaa aattaaacca actatataaa taattgcttg aagtacaggt aaagacataa
     1861 taatgataga tactcctacc actgcaataa taataagtaa gctgtctgac aacccggctg
     1921 taattattgc aggcaataca tatctatatt ttggttgatt agctccttgg ttaaaaataa
     1981 atacattttg tgcacctaaa ggtaaaatta gaccaatcgc taagataaat ccatgaataa
     2041 ttgcggttac cattaagttt tgacacccct aaaacataga
//
LOCUS       DAKTIP010000292         2074 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.297, whole genome shotgun sequence.
ACCESSION   DAKTIP010000292 DAKTIP010000000
VERSION     DAKTIP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.297"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            74..847
                     /gene="lpl1"
                     /locus_tag="O0305_002720"
     CDS             74..847
                     /gene="lpl1"
                     /locus_tag="O0305_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl1"
                     /protein_id="HCY0275816.1"
                     /translation="MGYLKRFALYISVMILIFAIAGCGKGNETKEDSKEEQIKKSFAI
                     TLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGHYFVTTFYRNGKLPDEKNYKIEMKNNKIILLDEVKDDKLKQKIENFKFFGQ
                     YANLKELRKYNNGDVSINENVPSYDVEYKMSNKDEIVKELRSRYNISTEKSPILKMHI
                     DGDLKGSSVGYRKLEIDFSKRENSKLSVIEFLSYKPAKK"
     gene            878..1678
                     /gene="lpl2"
                     /locus_tag="O0305_002721"
     CDS             878..1678
                     /gene="lpl2"
                     /locus_tag="O0305_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl2"
                     /protein_id="HCY0275817.1"
                     /translation="MRYLKKLAWFISVIILGIFIIGCDSSSDTGEKAKEDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDSEFKKGDKGMWMIYTDFAKSNKPGVLDNEGMIL
                     NLDRNTRTAKGYYFVDTIYDNHENSYSKNYRVEMKNNKIILLDKVEDQKLKERIENFK
                     FFGQYADFKSLKSYNNGDVSINSNVPSYDAKFKMSNKDENVKQLRSRYNIPTEKAPIL
                     KMHIDGDLKGSSVGYKKLEIDFSKEENSELSVVDSLNFQPAKKNKDDE"
     gene            1697..>2074
                     /locus_tag="O0305_002722"
     CDS             1697..>2074
                     /locus_tag="O0305_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0275818.1"
                     /translation="MWSIKRIGMYTNVIILSVFIIGCDSSSNNAENQREDSKEEQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFEKGDKGTWVLYSAMVSQPKGESLKSRGMIL
                     KLDRNKRTAKGSYIIRELKEDKNH"
BASE COUNT          882 a          203 c          415 g          574 t
ORIGIN      
        1 aatatacaaa ataattaatt atttatatga aaagagaata taaatgaagt ataaaacaga
       61 gagacgtgaa gcgatgggat atttaaaaag gtttgcattg tacataagcg ttatgatttt
      121 aatatttgcg atagcaggtt gtggcaaagg taatgaaaca aaagaagatt caaaggaaga
      181 acaaatcaaa aagagctttg cgataacatt agatatgtat ccaattaaga atctcgagga
      241 cttatatgac aaagaaggat atcgagatag cgaatttaaa aaaggtgaca aagggatgtg
      301 gacgatatat acagatttcg ccaaaagtaa taaaccgggt gaattggatg atgaaggtat
      361 ggttttaaat ctggatagga atactagaac cgctaaaggc cattattttg ttactacatt
      421 ttaccggaat ggtaaactac cagatgaaaa gaattataaa attgaaatga aaaataataa
      481 gattatttta ttagatgaag taaaagatga taagctcaag cagaaaatag aaaactttaa
      541 atttttcggt caatatgcaa atcttaaaga attgagaaaa tataataatg gtgatgtttc
      601 aattaatgaa aatgttccta gttatgatgt tgaatacaaa atgagcaata aagatgaaat
      661 agttaaggaa ttaagaagtc gttataatat ttcgactgaa aaatcaccta tattaaaaat
      721 gcatattgat ggggacctga aaggcagttc tgtaggttat agaaagttag aaattgactt
      781 ttcaaaacgt gaaaacagca aattatcagt cattgaattt ttaagttata aaccagcgaa
      841 aaaatagtat tgataaggaa tattagggtg tgaaaaaatg cgatatctaa aaaagcttgc
      901 atggttcata agtgttatta ttttgggcat ttttataata ggttgtgata gttcaagcga
      961 tactggggaa aaagcaaaag aagattcaaa ggaagaacaa atcaaaaaga gctttgcgaa
     1021 aacattagat atgtatccaa ttaagaatct cgaggactta tatgacaaag aaggatatcg
     1081 agatagcgaa tttaaaaaag gtgacaaagg gatgtggatg atatatacag atttcgccaa
     1141 aagtaataaa ccgggtgtat tagataatga aggtatgatt ttaaatttgg atagaaatac
     1201 acgtacggcc aagggatatt attttgtaga tactatatat gacaatcatg aaaactctta
     1261 tagtaaaaat tatagagttg agatgaaaaa caataaaatt attttattag acaaggtgga
     1321 agatcaaaaa cttaaagaaa gaatagaaaa ctttaaattt ttcggacaat atgccgattt
     1381 caagagtttg aaaagttaca acaatggcga cgtttcaatt aatagtaatg ttccaagtta
     1441 tgacgcgaaa tttaaaatga gtaataaaga tgaaaatgtt aagcaattaa gaagtcgtta
     1501 taacattcct actgaaaaag ctccaatatt aaaaatgcat attgatgggg acttaaaagg
     1561 cagttccgtt ggatataaaa agttagaaat agacttttca aaagaagaaa atagcgaatt
     1621 atcagtagtc gattcattaa attttcagcc tgccaaaaaa aataaagatg atgaatgata
     1681 gggaggatgt gaaacaatgt ggtcaataaa aagaattgga atgtacacaa atgttataat
     1741 tttgagcgtt tttataatag gttgtgatag ttcaagcaat aatgcagaaa atcaaagaga
     1801 agactcaaaa gaagaacaaa tcaaaaagag ctttgcgaaa acgttagata tgtaccctat
     1861 taagaatctc gaggatttat atgacaaaga aggatatcgt gatggtgagt ttgaaaaagg
     1921 cgataagggg acgtgggtat tgtattcagc tatggtatca caaccaaaag gcgagagttt
     1981 aaaatcacga ggaatgatat taaagttaga tagaaataag agaactgcta aaggaagtta
     2041 tattattaga gaattgaaag aagataaaaa tcat
//
LOCUS       DAKTIP010000293         2056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.298, whole genome shotgun sequence.
ACCESSION   DAKTIP010000293 DAKTIP010000000
VERSION     DAKTIP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.298"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            615..809
                     /locus_tag="O0305_002723"
     CDS             615..809
                     /locus_tag="O0305_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCY0275819.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(1042..1893)
                     /locus_tag="O0305_002724"
     CDS             complement(1042..1893)
                     /locus_tag="O0305_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCY0275820.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT          789 a          301 c          323 g          643 t
ORIGIN      
        1 tagagttgtt atagtcgcta cgccactcat actagttact gggtggttgt ttttatgtta
       61 tattataaat gatcaaacca caccacctat taatttagga gtgtggttgt tttcaagggc
      121 aaaaaaaggg cataattttg aaacaaaggg caagggtatg aataataaat tatcttgaaa
      181 attttataaa tgcatatata acaatgtttt gattgccctt gaacttcctt gataatctgg
      241 ctaattattc ctgctaaata aatcatatta atggctcagt aacgtgatgt tgctgggctt
      301 tttaatttaa acaggtattt atatgatatt taggaatgag atgctattac ggataaagta
      361 aaatcctata tgcatgcgtg ctactcattt attcttattt ttaatttcac atggctagtg
      421 actaatacat cataaatcat tagtgattct ttattatttc agtcccactc cctcaagtta
      481 atgatgaatc tatttcgcta gttataaaat gacgtcaaat caatatcaaa aattatttaa
      541 gtaaaatgtt tagataattt ttcagtgggt aagtattata tataacattt aattattccg
      601 aggaggcatt tattatggca gacgaaagta aatttgaaca agcaaaaggt aatgttaaag
      661 aaacagtagg taatgttact gataataaaa atttagaaaa cgaaggtaaa gaagataaag
      721 cttctggtaa agcgaaagaa ttcgttgaaa atgcaaaaga aaaagcaact gattttattg
      781 ataaagtaaa aggtaacaaa ggcgagtaat tttacttttt attcctaata cactgcacat
      841 gccacttagt cggtataacc atcggctagg tggtttttgt attaaaaaag tggataccaa
      901 aatttattaa taattatttt aatgttagaa aaaaactaaa taaaaactcg ctaatgatat
      961 ccaataatat gtatacaaaa cgagacatat attgcatatg attaacgaga tactgaaaat
     1021 attttatcac ccctaaaatg attattcatt ttcagcggta attcgaccta aagtcaaact
     1081 tacaataaaa ccgatgataa atactactaa tgaaacgaac cacatcacga tattagttgg
     1141 taaacctgga aatactgcaa agagggagcc aacaacaaaa ccaatgatta atgcaaaagt
     1201 cattagttta tgatgtgtta ggaaatactg gataattttg cttgaaataa tgaatccagc
     1261 aagcacgcca aatccgactg caagtaatat aggaagacct gcaaagttaa gtttaacaac
     1321 ttcagatatt gctagcatga ccgtaccata gacgccaaat actaataaca taaatgaccc
     1381 tgaaatacct gggagtaaca tagcactaga tgcacacata cctgcaataa aatatttaat
     1441 aataagacta gttgatagag taagtgtttc tccagcatgt ttatcaccat tattcattaa
     1501 tgtaataaca attaagatag cgataccagc tataaccatc atgtaatgtt tagttgtaaa
     1561 tgacgtttta tagttagaaa ttttcaataa atatggaacg ataccaatga ttaatccacc
     1621 aaagaaaaac atagttggaa tatggtgttg gcttaataaa taattaaaaa gattacttag
     1681 tgatcccatt gccagtaaca ttccaattat aatggggatt aaaaatgtaa aacttggcca
     1741 aaaacgtcgt gagaatatgc cgctaattga agcgataaat tgattgtaaa tacctaacaa
     1801 taatgcgata gtcccaccgc taacaccagg taccaagtca ctcgttccca tagcaaaacc
     1861 ttttagaata ttaatccatt taaattgttg catgaataac tcctttcaaa cgattggaat
     1921 aaaatcataa atagcatcat accatattac aaatgtccta gtgaaatgat aacatatttt
     1981 aaattcataa aatccattga gaaattatgt gcacttatta tcatttatat ttttaaagag
     2041 agcggattag agttgt
//
LOCUS       DAKTIP010000294         2026 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.299, whole genome shotgun sequence.
ACCESSION   DAKTIP010000294 DAKTIP010000000
VERSION     DAKTIP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.299"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..456
                     /locus_tag="O0305_002725"
     CDS             <1..456
                     /locus_tag="O0305_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 family protein"
                     /protein_id="HCY0275821.1"
                     /translation="LYKFFKENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSL
                     KEYRKYYEPLIRKNDKEFKEGMERARKEVNYAANTDAVATLFSTKKNFTKDNTVDDVI
                     ELSDKLYNLKNKPDKSTITIQIGKPTINTKKAFYDDNRPIEYGVHSKDE"
     gene            449..2026
                     /locus_tag="O0305_002726"
                     /pseudo
     CDS             449..2026
                     /locus_tag="O0305_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          875 a          224 c          356 g          571 t
ORIGIN      
        1 ttatataaat tcttcaaaga aaatgaaaag aaatatcaat atacaggctt tacaaaagag
       61 gcaattaaca agacacaaaa tgtcggatat aaaaatgaat atttttatat tacatactct
      121 tctagaagtt taaaagaata tcgaaagtat tatgaaccac tgattcgaaa aaatgataaa
      181 gaatttaaag aaggaatgga acgagcaaga aaagaagtga attacgctgc aaatacagat
      241 gctgttgcta cacttttttc tactaagaaa aactttacta aagacaatac agtagatgat
      301 gtaatcgaac taagtgataa attatataat ttaaaaaata aaccagataa atctacaatc
      361 acaatacaaa tagggaaacc cactattaat actaagaaag ccttttatga tgataatcgt
      421 ccaatagaat atggggtgca cagtaaagat gaataaaatt aatgataggg atttaacaga
      481 attaagtagt tactgggttt atcaaaatat tgatataaaa aaagaattta aagttaatgg
      541 aaaaaggttt aaacaagtag acagttataa tgatgataag aatagtaatt tgaatggtgc
      601 tgctgatatt aaaatatatg agttattaga tgataaaagt aaaccaactg gtcaacagac
      661 aataatttat caaggaacat ctaatgaggc aattaatcca aataatccat taaaatcatc
      721 ggggtttgga gatgattggc tccaaaatgc taaattaatg aataatgata atgaaagcac
      781 agattattta aagcaaacag atcaattatc aaatcaatat aaaataaagt tagaagatgc
      841 agatagatta tcaaatagtg attttttaaa aaaatataga atggaatcaa gtaacttcaa
      901 aaacaaaacc attgtggcgg atggcggtaa ttcggaaggc ggtgcaggag caaaatatca
      961 aggagcgaaa catccgaatg aaaaagttgt tgctactgac tcagcaatga ttccttatgc
     1021 tgcttggcag aaatttgcta gaccacgctt tgataatatg attagtttta atagtaccaa
     1081 cgatttatta acatggttac aagatccatt catcaaagat atgccaggaa aacgcgttaa
     1141 cattaatgat ggtgtgccca ggttagatac tttaatagac agccatgtag gttataaaag
     1201 gaagttaaat agaaaagata acacatacga tactgtacca ctaatcaaaa taaagtcggt
     1261 aaaagataca gaaattaaaa atggaaaaaa gtaaaaaaga ctattaacat aacattagat
     1321 atggatgggc gaattccaat aaatgtttgg acaggagatt cgattgcacg ttctggaaga
     1381 ggaactttaa ttaaacttaa tttagaaaat cttgatgcgt tgagtaaact gattactggt
     1441 gaaacaagtg gtatgttagc agaatgcgta atctttttaa atgaaagttt taacatctca
     1501 gaaaatgaaa ataaaaattt tgcagataga aagaaacaat tatcagaagg atttaaggat
     1561 aagattaact tatttcagtt agaagaaatg gaaagaactt taattagtaa aataaactca
     1621 cttgaagaag ttgcagatga aacaatagaa agtattagtg ctgttaaaca cttattacct
     1681 gattttgcat tggatgcatt aaaagaaaga attaatgagt tgtttaaagg tataaaatct
     1741 tttatagaaa aagtgtatga tagtatagat aatgaaattt tagaaatttt caaaaatata
     1801 gatcacgact tcagagatgg agtatctgaa gaaatgatga aacatttgaa agtagtgaaa
     1861 cagaatatag accaaataaa aaatcaaaat gatatttatg gtaggcaaat tgcagatatt
     1921 agaagtatta tgaaacaaca agatgcaaca attttagatg gaaattttca aattaattgt
     1981 agcggcgaaa atatggtaca gggtctagtt ataccttcta attatt
//
LOCUS       DAKTIP010000295         2010 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.300, whole genome shotgun sequence.
ACCESSION   DAKTIP010000295 DAKTIP010000000
VERSION     DAKTIP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.300"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(530..1555)
                     /locus_tag="O0305_002727"
     CDS             complement(530..1555)
                     /locus_tag="O0305_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCY0275822.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            1825..>2010
                     /locus_tag="O0305_002728"
     CDS             1825..>2010
                     /locus_tag="O0305_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275823.1"
                     /translation="MEQIKLKTFTAETLELLEKNINAFLSSEEATNLKLVNITIKEIE
                     ERTFPNNEEEFNAILTLS"
BASE COUNT          721 a          348 c          248 g          693 t
ORIGIN      
        1 ataaagagaa agcggtgcta ttttttgcgc ttttataaca actaaggata caagacacat
       61 aacttcaaca atatcctcat ctaaacctat cgatttccta agctcaattt taaacacaaa
      121 gtttatttga aatgatgcac acctataaaa atcttaatca tcgaaaagtc acaaacatct
      181 ccatattaaa aacttaaata gtgcattcca tttaaactaa acatcgatat tcctaccaat
      241 atagacttag taattcgatt gttaatacat tactttcata aaataactac aaccatcata
      301 atacttctca aatgtcgtca aattaaagta aatacagcac aaattaacat ttttaaatat
      361 ataaaataaa acaagatact caataatctg tagtaccttg ttttggaata atgtaatttt
      421 atatcatcaa cggctacaaa tgagcagttt catatataaa taaacacatc tactcaactg
      481 atcttaatta attaataaat gatttaccag atgacatata acagcattat tagattaatt
      541 ctaatagttt atttaaattt tcttcggttg tcgcccaact ggttgcgaat ctaacaacac
      601 gatgttgatc atcgtatttt tcccaaacag caaatttaac tttttgttct aactctgcta
      661 ttttctcgtt acttaaaata aaaaattgtt gattggttgg agaatcaaag taaagacgat
      721 agcctttatt tttaaacccg tctttcatct tatttgccat ttcgatagca tgtctgctta
      781 tattaaaata taaattgtcc gtaaataatt ctaaaaattg tatgcctgtt aaccgtcctt
      841 ttgctaaaag ggcaccgtga tgcttgattc gagtggtaaa ttgtttcggt tcattatttt
      901 tcgtaaaaac aatggcttcc ccgcataatg cacctatctt cgtaccacct atataaaata
      961 catcacaata tttagcgatg tctttaatag tcatatctga ttggtcactc atcaatccat
     1021 accctaatcg tgcaccatcc ataaataatg gaagctgata ttgcttacat actttgcata
     1081 actcttccaa ttctgattta gagtataatg tgccatattc tgtaggatga gaaatatata
     1141 ccattcctgg gaataccata tggtcctttt taaaatcact tttaaatgtc tccatgtaag
     1201 tttcaacatc tgaagcacta acttttcctt ccttagaggg tatagtaatt actttatgtc
     1261 cactatattc aattgcaccg ccctcatgca cagcaacatg accagtgtct gctgaaatga
     1321 ccccttcgta actttctaac attgaattaa taacaacctg attggtttgt gttccaccta
     1381 ctaaaaaacg aattgtagca tttggacagt caattgtatc tttaatcttt tcaattgcct
     1441 gagctgtgaa ttgatcaaag ccatatcccg aagcttgtac aagatttgta tctactaatc
     1501 gttttaatac tttttcatga gcaccttcta aataatcatt ttcgaatgaa atcactacat
     1561 ttccccctaa aactaatatc aacattttaa taagataaac caatttcaaa actagttcga
     1621 tatttaaaat gtattatgaa tggttaaagt ttgtatcgca ttatcgcgaa gttgaataaa
     1681 tatattatac atcaaacaat acattatgaa aattaattaa ttttttatca cagcgacttt
     1741 tcatgctgga acacatgtat taatgattga attttactgt taaaagttct atgatttagg
     1801 aaatagagaa aagaggggat ttaaatggaa caaattaaac ttaaaacttt tacagctgag
     1861 actttagagt tattagaaaa aaacattaat gcttttttaa gttctgaaga agctacaaat
     1921 ttaaaattag taaatattac tattaaagaa atagaagaaa gaacattccc aaataatgaa
     1981 gaagaattca atgcaatttt aactttatct
//
LOCUS       DAKTIP010000296         1996 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.301, whole genome shotgun sequence.
ACCESSION   DAKTIP010000296 DAKTIP010000000
VERSION     DAKTIP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.301"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0305_002729"
     CDS             complement(<1..338)
                     /gene="deoC"
                     /locus_tag="O0305_002729"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCY0275824.1"
                     /translation="MNSAKLIDHTLLKPESTRTQIDQIIDEAKAYHFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEAVV"
     gene            653..1363
                     /gene="deoD"
                     /locus_tag="O0305_002730"
     CDS             653..1363
                     /gene="deoD"
                     /locus_tag="O0305_002730"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCY0275825.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1484..1927)
                     /locus_tag="O0305_002731"
     CDS             complement(1484..1927)
                     /locus_tag="O0305_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCY0275826.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
BASE COUNT          623 a          351 c          312 g          710 t
ORIGIN      
        1 accactgctt caatgtcttg ttgtacatca tcaaaacgtc catcttttaa tgcgccgatg
       61 ttgatgacca tgtcaatttc atctgcacca ttttgaatcg catcttctgt ttcaaatgct
      121 ttcgtcgcag ttgtcgatgc acctaatggg aatcctatta ccgtacaaac taacacctct
      181 gaatcagcta gtcgctctgc tgcatattta acatgcgttg gattcacaca tacagattta
      241 aaatggtatg ctttcgcttc atcgatgatt tgatcgattt gcgtacgtgt tgactcaggc
      301 ttcaataaag tgtgatcaat caattttgca ctattcattt tctatctcct cctttatggt
      361 tgattataaa aatacggttg taaattagtt gattgagcgt caggttcatt taaatatcag
      421 gttagatgtt cgctttttat gtaaccgcat acatatacta ttacattaat tcatttccca
      481 taaacaaaca atacaattga acgtgatatc ttcattatga acgatgactt gacaacaagc
      541 taatcaggat tatattttta taattcttta attctatagt acaaaaattc gcaaaaaagg
      601 gaaacaaatg ttatcttaaa attattaatg aatattaagg agaagataac aaatgacaaa
      661 aggtacacca catattcaac caaatggagt aaaaattgct aaaacagtat taatgcctgg
      721 cgatccgcta cgtgcaaaat atattgctga taatttttta gaaaatgttg aacaatttaa
      781 cgatgtacgt aacatgtttg gttacactgg tacatataaa ggtaaagaag tttctgtaat
      841 gggttctggt atgggtattc caagtattgg tatttactca tatgagttat acaacttctt
      901 tgatgtagat acaatcattc gtatcggttc ttgtggcgca ttacaagaaa atgttaactt
      961 atacgatgtt attattgcac aagctgcatc aactaattca aattatgtag atcaatacaa
     1021 tattccaggt catttcgcgc ctatcgctga cttcgagtta gtaactaaag ctaaaaatgt
     1081 cgctgaccaa atcggtgcta ctacacacgt aggtaacgta ttatcttctg atacatttta
     1141 caatgccgat ccaacattca atgatgcttg gaaaaaaatg ggtattttag gtatcgaaat
     1201 ggaatcagct ggtttatatt taaatgcgat tcatgctggt aaaaaagcac ttggtatttt
     1261 cacagtaagt gatcatattt tacgtgacga agctactaca cctgaagaac gtcaaaattc
     1321 atttacacaa atgatggaaa tcgctttaga aatcgcagag taacttattt aaattgactt
     1381 taattgctct ttaacaatgc gattaaactc aaaaagccaa cacattctgg gcgtatcccc
     1441 atttatgtgt tggcttttat ttatattatt acttatctgt agattagctt aagtaagatt
     1501 taaacatcca attatgttta tctactgatg tttgcatacc tataaacata tcttctgata
     1561 catcatcgcc agcattacca gcaatttcga ttgcgttttc taattgtttt gagatatttg
     1621 tgaagtcttg tgataattct tcaaccattt gttctgcaga gtaacctttc gcagcttctt
     1681 taacaattga ttgctctaag cattcagtta atgtacctac agggtttcct cctaccgcta
     1741 aaattctttc agctaattcg tctacatatt ggcttgcttc attatataat tcttcaaatt
     1801 taacgtgtaa tgagaagaag ttaggccctt tcacatacca gtggaaattg tgtagctttg
     1861 tgtaagctac tgtccagttt gctacttgtt gattcaattc ttttacaaca tcttgttgat
     1921 tactcatatt taatacactc cttaaaattg tctacgtctt gcttgtttat attaattatt
     1981 atattataat aattct
//
LOCUS       DAKTIP010000297         1996 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.302, whole genome shotgun sequence.
ACCESSION   DAKTIP010000297 DAKTIP010000000
VERSION     DAKTIP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.302"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            565..1434
                     /locus_tag="O0305_002732"
     CDS             565..1434
                     /locus_tag="O0305_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0275827.1"
                     /translation="MEMSRLNLTDDNDIDEIIAWRGEQEELEQKRDTYKKRYHEFEME
                     IARLESLTKDKELLDSDKLKDEYELKKGKMNTLIDEYSAVHYQCQNNINKTQSIVSHI
                     NYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFVLIYYLDQIIAQANLRLATMSDNRY
                     QLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLSGGETFQSSLALALGLSEIVQQQSG
                     GISLESIFIDEGFGTLDQETLETALDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVK
                     SDQYQSSTRFKRN"
     gene            complement(1505..1867)
                     /gene="mscL"
                     /locus_tag="O0305_002733"
     CDS             complement(1505..1867)
                     /gene="mscL"
                     /locus_tag="O0305_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCY0275828.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT          796 a          273 c          315 g          612 t
ORIGIN      
        1 gattttgaca gtattgctaa acgtcaaaat gaaattaaag aaattgaagc aaatatccac
       61 gctataaaat cgaatattgc tgtgcataat tctgaaatta aatttgttaa tgaaaaaata
      121 tcgaatatta atattaaaac gcaaagtgat ttttcacttg aagtattgaa taagcgtctg
      181 ctagaaaatg aaaatgcatt gaataatcaa agagatctta ataaatttat agaacaaatg
      241 aaagaagaaa aagataatct aacgttgcaa attcataata aacaattgcg tctaaataaa
      301 aatgaatctg agttgaaatt atgtcgagat ctcatcactg aatttgaaac actctcgaaa
      361 tataataata tcactaattt tgaggtggat tataagaagt atgttcaaga tgtgaatcag
      421 catcaagaac tctcaaagga gattgaagat aagttaatgc aattgtctca aagaaagtta
      481 attgagcaaa ataatctaaa tcattatgaa aatcaactag aaacttacaa taatgactta
      541 gaattgaatg aacagtctat tgaaatggaa atgtcgaggc tgaatttaac tgatgacaat
      601 gatatagatg aaataatagc ctggaggggc gagcaagagg aattagagca gaaaagggat
      661 acttataaaa aacgttatca tgaatttgaa atggaaatag ctaggttaga atcattaact
      721 aaggataaag agttattgga ctctgataaa ttaaaagatg aatatgagct aaaaaaagga
      781 aagatgaata cactgataga tgaatactct gctgttcatt atcaatgtca aaataatatt
      841 aataaaacac aatctatagt ttcgcatatt aattacttaa atcaagaatt aaaggatcaa
      901 caagaaatat ttcaattggc tgaaattgtc agtggtaaga ataacaaaaa tcttacattg
      961 gaaaactttg tcttaattta ctatttagat caaattattg ctcaagcaaa tctgagatta
     1021 gcaacaatgt cagataatcg ataccaacta attaggcgag aagcggtttc tcatggtctt
     1081 agtggcctag aaattgatgt atttgatttg cattcaaata agtctagaca tattagctcg
     1141 ttatcaggtg gagaaacttt ccaatcgtcg cttgcattag ctttagggtt aagcgaaatt
     1201 gtacagcagc aatcaggagg tatttcacta gaatcaatat ttattgatga aggattcggt
     1261 acattagatc aagaaacgct tgaaacagcg ttagacactt tattaaatct taaatcaact
     1321 ggtagaatgg ttgggattat ttcacatgtg agcgaattga aaaatagaat acctttagtt
     1381 ttagaagtga aatcagatca atatcagagt tcaacaagat tcaaaagaaa ttaaaacata
     1441 aaaaatagag atggtacttt ggaatgtata gaacaacctt agcccatctc tttttattta
     1501 aaaattattt tttctcacgt aataaatctc tgatttcagt taataacaca acattttctt
     1561 ccacaactgc ttcttcttcg gcttcttctt tcttcattaa tgtatttgca atcttaacaa
     1621 agatgaataa agcaaacgcg atgataataa agtcgataac agattggata aataaaccgt
     1681 atttaatacc ccagaatgac cattctttag caaaatcaac tgatccgaaa attttaccaa
     1741 ttaatggcat aatgatattt tctactaatg aagatataat cttgttgaaa gctgcaccca
     1801 tcacaacagc aattgctaaa tctaagacgt taccttttaa ggcgaactct ttgaattctt
     1861 ttaacattac actcaacctc tcttttttaa atgtaacctc aattatacaa caaataaaaa
     1921 aattagaaaa tagattattc aagaaaattt gattatcgca aagacctttg ttatcctata
     1981 aaggtgcgtg agttat
//
LOCUS       DAKTIP010000298         1990 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.303, whole genome shotgun sequence.
ACCESSION   DAKTIP010000298 DAKTIP010000000
VERSION     DAKTIP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.303"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..285
                     /locus_tag="O0305_002734"
     CDS             <1..285
                     /locus_tag="O0305_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HCY0275829.1"
                     /translation="VKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQRDLE
                     KIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            263..577
                     /locus_tag="O0305_002735"
     CDS             263..577
                     /locus_tag="O0305_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCY0275830.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            592..>1990
                     /locus_tag="O0305_002736"
     CDS             592..>1990
                     /locus_tag="O0305_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275831.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIV"
BASE COUNT          770 a          247 c          357 g          616 t
ORIGIN      
        1 gttaaagaaa aagtaatgca atctttaatt caaaaagggt ttgaaatgga aaccattcat
       61 gctgtattga atgaaatgga ttttacacaa gatgaagcgg ttttagacga tttattacaa
      121 cgagatttag aaaaaattta taataaaaat cgaaagaagt acacgcaaca gaaattaatt
      181 tcaaaaacta tcgaaggcct tatgagaaaa ggatataaat atgataaaat taaagctaaa
      241 ttagaagaaa gtggtattgc cgatggaaca gaagaaattg agtgagatgt ctgagccaga
      301 attaagacac gaaatacaac tttataaaga gaaaatgaga aaagctgaga tgaatggtat
      361 tttaaatgaa tatgacgttt atcaaagtaa agtaattgtt gccgaaagtt acttagtaga
      421 cagaaaaaag atagaaattg gtaaaattta taaactgact gatggcagta atcaatattt
      481 taaagtagaa agattaaaag gtatttttgc gtgggggttt agatttaata gcgatgaacc
      541 cgaagaagga ttacctatag cgttattaca attatagaag gatgaaaata gatgggaagt
      601 tcaattgttt taaagttact aaaagtaaca cactactata ggaataaaca gaataagaaa
      661 tggtatttac cttttggata tgatgctgaa gatatcgatt taaacaatat tagtttacat
      721 atttatcaag gagaagcatt aggtataatt ggtgaacctg aatcttccaa agcgttggta
      781 ggtcaattgt tggcaggtgc aattaaacct gataaaggta aagtagtttg tactgaagat
      841 ttgttctacg gatatattga agatcaatcg ttaattcatc aaactgttga agcttataca
      901 gcgcagttag ttcaactatt tccatatgaa attaatgatc ataaagctga acagattatt
      961 caatatgcac atttaggtga ttataaaacg aagccggtta accatatttc gaaagcggca
     1021 tacgctcaat tactattaag tattgcacgc tcatcaaaat caaatattat tattttaaat
     1081 catgttattg actatttaac accacaattt atggaacgtg cgattgaatt aacaaatgat
     1141 tatattgaaa ataatttaac gattgtgtca attggtgatg atattgataa aatttcacaa
     1201 gtgagtaact acatagcttg gttttcacat ggtcaattaa gaatggaagg gtcacttaaa
     1261 caagttattc catcttttaa agaacatgaa cgtgatcgat taagtctaaa ctcaaaagaa
     1321 gaaattgaaa actttgattt agattggaaa aagaatcgta caagaatacc agagatgacc
     1381 tataatttca aacgtgttga gcgctataat catgcaaaac cgcctaagtt tttagtgcgt
     1441 ttttggactt tggcctcagg tactatttta ggcttagcat tgatgatgtt gctcattttc
     1501 aataatatag gaattatttc gataacagat tttacgaatc gtgctacgat gcaaaatgaa
     1561 aataaagatc catatggcga aaagttagct tatggaattg cttttaatgg cagtgtggat
     1621 atgcaagggg ataaacaagt cacaattcca aaatatagtg tagttacaat tactggcgaa
     1681 aatagtaaaa attatcgtgt taccgccgat aataagactt actatgttag taaagataaa
     1741 ttagaatatt ttaacccggc aggtttatat caaacgcata gttttaaaaa attagcacca
     1801 tatatgaaat caaattatag taattactat gcatacttta atagtcaatt acataaaaag
     1861 catagttcag ttataaaaac tttagttcct gatgatgata accgtttcgt tgcatccgtt
     1921 acacaacaac cgatacaatt acttttcaat gataataatc agttatacgg ttttgtttat
     1981 ccaattgtag
//
LOCUS       DAKTIP010000299         1965 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.304, whole genome shotgun sequence.
ACCESSION   DAKTIP010000299 DAKTIP010000000
VERSION     DAKTIP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.304"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            31..>1965
                     /gene="recJ"
                     /locus_tag="O0305_002737"
     CDS             31..>1965
                     /gene="recJ"
                     /locus_tag="O0305_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCY0275832.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVIT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHS"
BASE COUNT          737 a          282 c          370 g          576 t
ORIGIN      
        1 atgtcttttg gaaatgaggc gtaaatatat atgattaaac cgaagtataa gtggaaatta
       61 actaaaccag ctgaatacat aagtgatgaa ttaacatcaa aattaaagtt aacgccaatc
      121 gtaaaaaaaa tattagaaag taaatcgatt attgatgaac aagcaataga atccatcatt
      181 tcagatacag atatcaatca tgatgcactg caattgagcg atatgactaa gactattgaa
      241 agaattaaaa gagcgatagc gaatgatgag aaaatcctag tttatggtga ttacgatgcc
      301 gacggtgtta ccgcaactac aattttagtg ataacattac aacttttagg tgcacaagta
      361 ggatggcata ttcctaaccg ctttaccgaa ggatatggac caaacgaact agcatttaga
      421 aatgctcatg atgaaggcat aacattaatc ataactgttg ataatggcat acaaggtcat
      481 aatgaaatta aaatggttca agatttagga gttgatgtca ttgtaactga tcatcatgaa
      541 attggtagta cattaccaga agcatatgca atcgtacatc caatgcatcc atcgtttaat
      601 tatccgttcc aacaattgtg tggtgcaggt gttgcataca aattagcaca agcattgatt
      661 gaaaatgtac ctgattattt taaagcatta gttgcgatag gtaccattgc ggatttagta
      721 tccttaactg atgaaaatcg atcattagtg aaacaaggat taaaagtctt aaatgatcaa
      781 tgtccgacct ctgttaaagc attgctaaaa gaagcgggat acaatgacaa tattgatgaa
      841 gaaacaattg gttttattat tggacctaga cttaatgctg taggtcgttt agatgacgca
      901 tcattagctt gcgaattgtt gatgactgac gtcgaagaag aagctgcatt tttagcagaa
      961 caagttgaac attttaaccg tgaaagaaaa gatattgtag caactatcac agaagaagca
     1021 atggctatgg cagaaacgaa agttaaaaag ggagatttat ttttactttt agcaaaagaa
     1081 aattggcatg aaggtgtctt aggtattgtt gcttcgaaaa ttgtcgaaac atttgctttg
     1141 ccaacattaa ttttaaatat tgatagggag caaaatcatg caaaaggctc cgccagatca
     1201 attgatcaag tgtcgatgtt tgaaatacta tcagcacatc aagagttaat cgctaaattt
     1261 ggcggccatc atatggctgc aggtatgacg atggatattg aaaatataga atcgttagct
     1321 gaaggtttaa ataaatggat gaaagaatta tctaaaacaa cttcgttaga tcccgtaaag
     1381 caagtagatg tattattaac agaaaatgat ataacgatta aaaatattcg tgatatgaat
     1441 cgtttaagac ccttcggaac agatttttct agacctattt ttgaaatgga tgatttatca
     1501 gtttcttcag ttaaggcgat aggtcaacaa aaaaatcatc taaaattgac acttggtgaa
     1561 agtaatatcg ccgcgctatt ttggcaaaat ggacacttag aacctgagtt acaagatgaa
     1621 cagccaatta atatattagg atctgttcaa atcaacgaat ggaatggtaa tcaatcaccg
     1681 caaataatta ttcaagatat tgcgatgaat gaacagcaaa tattagatta tagaagtaag
     1741 cgaaaaagtt taccttttac agaaaatgat gaaaatattg tcgtgcttat tcatcctaaa
     1801 agtgataaag taaatgcgaa tgaatattat tatggtgaag aaattaaaca acaaactgat
     1861 aaagtagtat taagagattt accaacgtca atggaagact tgtctaattc cttgcaacaa
     1921 ctgcaatttt ctcaacttta tatagttttg caacataatc attcg
//
LOCUS       DAKTIP010000300         1960 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.305, whole genome shotgun sequence.
ACCESSION   DAKTIP010000300 DAKTIP010000000
VERSION     DAKTIP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.305"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            106..1026
                     /gene="rnz"
                     /locus_tag="O0305_002738"
     CDS             106..1026
                     /gene="rnz"
                     /locus_tag="O0305_002738"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCY0275833.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKI"
     gene            complement(1131..1946)
                     /gene="proC"
                     /locus_tag="O0305_002739"
     CDS             complement(1131..1946)
                     /gene="proC"
                     /locus_tag="O0305_002739"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCY0275834.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
BASE COUNT          666 a          308 c          339 g          647 t
ORIGIN      
        1 aactagcaaa gacgaatttt taacagattt tgattcaagt ataaattaaa actaaattga
       61 tacaaatttt atgataaaat gaattgaaga aaaggagggg catatatgga agttacattt
      121 tttggaacga gtgcaggttt gcctacaaaa gagagaaata cacaagcaat cgccttaaat
      181 ttagaaccat attccaattc catatggctt ttcgacgttg gtgaaggtac acagcaccaa
      241 attttacatc atgcaattaa attaggaaaa gtgacacata tatttattac tcatatgcat
      301 ggcgatcata tttttggttt gccaggatta ctttctagtc gttcttttca gggcggtgaa
      361 cagaagccgc ttacattggt tggaccaaaa ggaattaaag catatgtgga aatgtctatg
      421 aatttatcag agtcacattt aaactatcca attacatata ttgaaatcga tgatcatctg
      481 acatatcatc atgatggttt tactgtagag gcgcatttac ttaatcatgg tattccatca
      541 tatggatata gagtaatggc acctgaaact acaggtacaa tcaatgttga agcgttaaaa
      601 aacattggtc ttgaaccagg tcctaaatat caagaagtaa aatctcatga tacatttgaa
      661 cataatggtc aagtttatca atcaaaagat tttagaggtg agtccaaaca gggtccagtt
      721 gtagcaattt ttggtgatac aaaaccatgt tctaatgaac gtgtgatttc tagagatgct
      781 gatgtgatgg tacatgaagc gacctatata gatggtgaaa agcatttagc caacaattat
      841 catcatagcc atattgaaga cgtatttgca ttgataaaag aagccaatgt taaacgtacg
      901 ttgattacac atttgagcaa tcgctataat acagaagata taaatgaaat ttatcaaaca
      961 ttgatacaaa atgaagatac acctaatttt aattttgtaa aagattttga tagttttaaa
     1021 atctaatgac aaagtaataa acaatgcttg ccaaataaca ataaattata aaaataaccc
     1081 gtcaaacaca gtgcttgcga tgatgcatac atgtgttggc gggtttgttt ttattggtct
     1141 tctatattag aaagttcaat actacggtcg acggcagcgt ttagacaatc ttcgaaaata
     1201 gatactaaat catattgtga caatgtatca aggccagctt gtgtcgtacc acctttagag
     1261 gtaatatttt ttcttaattg agccatgctc aaatctgaac gttcaatcat cttacttgta
     1321 cctataataa ggttgcgtat agattcttca acttgttctt tttctagacc aagtttcgta
     1381 ccagctttaa catattgctc gaatacatga tataaaaatg ctgggccgct tccggtgata
     1441 gctgttactt gatgtaaatg atcttctgat acttcaatta cagaaccaaa tgctttaact
     1501 aaatcgttaa tttcatcttt agatttaggg tcaaagttgt ttgaaaaact aatgccagta
     1561 acagagtgtc caacttgcgc atttgtgttt ggcataattc tagcaactgg attttggcat
     1621 tctaattgtt gtttaatata atcaatcgga atacctgcca taattgaaat gaagcaattg
     1681 tcttttgtaa tatgtggttt gatgcgtgtt gctagagcat caaaatcatg tggtttggta
     1741 cctaaaaata cataatctgc atcttttaat aatgtcgcat catcataact atagttaaca
     1801 cctagttttt cagcgaatgc ttttaaagct tgttcattag atttatttgt taaatatata
     1861 tcattggcat ctaagttgct tgagttaata attcctgtaa atatagcttg tgccatatta
     1921 ccagctccat aaaatacgag tttcattaac gtcacatccc
//
LOCUS       DAKTIP010000301         1950 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.306, whole genome shotgun sequence.
ACCESSION   DAKTIP010000301 DAKTIP010000000
VERSION     DAKTIP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.306"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..206
                     /locus_tag="O0305_002740"
     CDS             <1..206
                     /locus_tag="O0305_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275835.1"
                     /translation="YKYFPNNDENIKPGIIQMDIDSLEVINAEKSSLEKNTRDNYFIH
                     AIDRIYINTSKGLFPESELVAWG"
     gene            241..363
                     /locus_tag="O0305_002741"
     CDS             241..363
                     /locus_tag="O0305_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275836.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            339..437
                     /locus_tag="O0305_002742"
                     /pseudo
     CDS             339..437
                     /locus_tag="O0305_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            746..883
                     /locus_tag="O0305_002743"
     CDS             746..883
                     /locus_tag="O0305_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275837.1"
                     /translation="MILIMLDSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKD
                     K"
     gene            945..1205
                     /locus_tag="O0305_002744"
                     /pseudo
     CDS             945..1205
                     /locus_tag="O0305_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1254..1602
                     /locus_tag="O0305_002745"
                     /pseudo
     CDS             1254..1602
                     /locus_tag="O0305_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1613..1807
                     /locus_tag="O0305_002746"
     CDS             1613..1807
                     /locus_tag="O0305_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275838.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
BASE COUNT          819 a          207 c          330 g          594 t
ORIGIN      
        1 catataaata ttttcctaat aatgatgaaa atattaagcc tggtattatt caaatggata
       61 tagatagtct tgaagttatc aatgctgaaa aatctagttt agaaaaaaat acaagagata
      121 attattttat tcatgccata gacagaatat acataaatac tagtaaaggg ctatttcctg
      181 aatctgaact cgtagcatgg ggataaagaa agtttacttt aaaaatttta accttcaaat
      241 gtgaaggtta ttttttattg cccaaaacgt gctgatgaca ttttaaaagc aagtatggaa
      301 tatcagtcga cagactataa acggaggtat atctcatgga aaacaatgat agcaatgaag
      361 tagcttattt gttaaaagat ggtaaattta caaaagtata tgtcaatcaa gatagtgtat
      421 cttttgtgcc agggtaacaa gcaaccgaat tattatttaa cagtaaaccg aattcaattg
      481 ttatgttaca taaccatcct ggacagtcag gattttcaga atatgattta tttacatttt
      541 ttaagcatcc atcaataaaa tcaatgacaa tcgttactaa taagggacaa gtaaaattta
      601 ttaccaagtc aaataggttt catggtaaaa tagtgagtaa attttgtgct aaatatttta
      661 cgcatattaa tatcattaat gatagccata ttgaaaaatt attgaaaaaa ctttatagta
      721 taaatatgat taaatataaa gtgagatgat tttaattatg ttagattcac aattagatgg
      781 aaatgtaact gcaaaagaat tgagtgaatc ttttaaagaa ttagttgaag aatttgaaag
      841 tatagaaaaa gcgaaaaaga attcaaattc aaaagataaa tagcatcctt tttagacaga
      901 taaagagaaa gtggtgctat ttttatacgc ttttttaact atttactgaa aatgcagaag
      961 cacaaaagtt aacatattta aaagaacttg gtgaagatgg cgaatataaa tatgttgcca
     1021 aaatagatag taaaacatct aaattatgtc attcacttaa cggaaaaata tttaaagtta
     1081 aagatatgat accaggtgtg aatgcgccac ctatgcatcc ttggtgtaga agtaccacag
     1141 tgccacatgt tggcaattgg cgggacaagt tctttaaaga gcgtgaaggt aaatatcaag
     1201 tagaagtaaa agaagcataa ttacaggaaa aagctaaaaa ccagatgaaa gaaatgatta
     1261 aaaatggtaa aataaaaata gaaataaatt gcggaaaaat aaaatagata tatgttaggt
     1321 catcacctat ataatgaaaa taaaaaaaga gccattttaa ataataagaa attgcctagc
     1381 tatacaatac tttctataga tttattgaat gaattgttaa gagaaaaaat gtcaacaggc
     1441 aatctaatat taagtgatga gcgatttgat ttgaaagaga ttattaattt taatcaaatt
     1501 attggaaaaa tacatatcga aaatgtgtat attgaaacca gaaagggaaa agtgcattat
     1561 tcgaagacag gtgctcatat agtaccttat attgataagt aggtgaaaag tattgagaat
     1621 tgaagatgca tatcgtaaag atgttattat cacgcttttg aataatgaag aatacgaagg
     1681 gtttgtaact gactatgaaa atgaattcga gagcgaaaca ggaaatcttg ttgtagatat
     1741 acagactgat tttgctgttt attcgtttga tgaaactgag ataaaaagta ttagattatt
     1801 aaaataatta caaaatagca tcctttctac aaaaagctaa aaaagaaatg cttgaaatga
     1861 taagtaatgg taaaatgacg atgaaattga ataatgttaa gcaaaagcgt catattttat
     1921 gcacaaatga atataataat aagaaaaata
//
LOCUS       DAKTIP010000302         1941 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.307, whole genome shotgun sequence.
ACCESSION   DAKTIP010000302 DAKTIP010000000
VERSION     DAKTIP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.307"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..333)
                     /locus_tag="O0305_002747"
     CDS             complement(<1..333)
                     /locus_tag="O0305_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0275839.1"
                     /translation="MKTNILNLDNELENTEYVEGKPYVQYGIKYERNQALRNEAIKIH
                     GTTCKVCGFDFKAKYGDLGEGFIEIHHLKPMFSIKREIKVNPQKDLVPLCSNCHKMIH
                     RNTKKPLTI"
     gene            complement(330..467)
                     /locus_tag="O0305_002748"
     CDS             complement(330..467)
                     /locus_tag="O0305_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001791883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275840.1"
                     /translation="MELKLLNKLDDDQLDLEFLQDNGLKGNVQGPMTLKEPLKSYIQK
                     S"
     gene            523..1841
                     /gene="cstB"
                     /locus_tag="O0305_002749"
                     /pseudo
     CDS             523..1841
                     /gene="cstB"
                     /locus_tag="O0305_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002488497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
BASE COUNT          663 a          310 c          291 g          677 t
ORIGIN      
        1 aatcgttaaa ggttttttag tatttctatg aatcatttta tggcaattag aacatagtgg
       61 gactaaatct ttttgtggat ttacttttat ttctcttttt attgaaaaca ttggttttaa
      121 atgatgaatt tcaataaaac cctcacctaa atcgccatac ttagctttaa aatcaaatcc
      181 acatacttta catgtagttc catgaatttt aatagcttca tttcttaatg cctgatttct
      241 ttcatattta ataccgtact gtacatatgg tttaccttcg acatattcag tgttttctaa
      301 ctcattatct aaatttaaaa tattcgtttt caagactttt gtatataact ttttaaaggt
      361 tcctttaaag tcataggacc ttgaacatta ccttttaacc cattatcttg taaaaattct
      421 aaatctaatt ggtcatcatc aagtttgttt aataacttta attccatgta attaccatag
      481 ttaatataat tatcaccctt gataacaaat tgactatcat caatttatga taaacattta
      541 tctcaagcat cttatttaat cggttgtcaa aaaactggag aagccatgat cattgatcct
      601 atttgtgact tatcttcata tattcgagtt gccgatgaag agagtttaac cattactcat
      661 gcagctgaaa cacatataca tgcaaatttt gtttcaggaa ttagagatgt tgctataaag
      721 ttaaatgcta gtatttatgt atcgggcgaa agtgatgaca tgttaggtta taaaaatatg
      781 cctaaccaca ctcattttgt tcaacataat gatgatattt atgtaggaaa tataaaatta
      841 aaagtgcttc atacacctgg tcacacgcca gaaagtataa gttttttact tactgacgaa
      901 ggtgctggag cacaagttcc aatgggacta ttcagtggtg attttatttt tgtaggagat
      961 atcggtagac ctgatttact agaaaaagct gttaaagtag aaggattatc tgaaataggc
     1021 gcaaaacaaa tgtttaaatc tattgaaagt attaaagact tgccagacta cattcaaatt
     1081 tggcctggtc atggcgctgg tagtccttgt ggtaaatctt taggtgctat tccaacatct
     1141 acgcttggct atgaaaaaca aacgaactgg gctttttctg aaaataatga agctaccttt
     1201 atcgataaac tcatttctga ccaacctgca ccaccacatc attttgcaca aatgaaaaaa
     1261 attaatcaat tcggtatgaa tttatatcaa ccttatacgg tttatccagc tacaagtaca
     1321 aacagattaa cttttgatct ccgcagtaag gaggcttatc atggtggaca tattgaaggt
     1381 acaatcaata ttccatatga taaaaatttc atcaatcaaa ttggctggta tctaaactat
     1441 aatcaagaaa ttaacttgat tggagactat caccttgttt caaaagcaac acataacata
     1501 ctttacaact tattggatat gataatgtgt ctggatatca gttaccacaa gctcaaattc
     1561 aaacacaatc tgttcatagt aaagacataa ctggtaatga agcacatgtc ttagatgtac
     1621 gtaatgataa tgaatggaat aatggccact tatcccaagc ggttcatgtg ccacacggca
     1681 aacttttaga aacagattta cctttcaata gaaacgatgt tatttatgta gactgtcagt
     1741 ctggcattag aagttctata gctattggta ttttagaaca caaaggttat cacaacatta
     1801 ttaatgtaaa cgaaggttac aaagatatat acctttctta ataattatat ttatactaat
     1861 gaagaaggac tataagtagt tagatatagc ttttagatat agcttatagt tcctttttat
     1921 attattggat aaatatctat t
//
LOCUS       DAKTIP010000303         1941 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.308, whole genome shotgun sequence.
ACCESSION   DAKTIP010000303 DAKTIP010000000
VERSION     DAKTIP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.308"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(298..1158)
                     /locus_tag="O0305_002750"
     CDS             complement(298..1158)
                     /locus_tag="O0305_002750"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCY0275841.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            1376..1897
                     /locus_tag="O0305_002751"
     CDS             1376..1897
                     /locus_tag="O0305_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCY0275842.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
BASE COUNT          622 a          339 c          284 g          696 t
ORIGIN      
        1 ccccacttga ccatggtcta aagtctgatt attcaactaa atattgacgt tgttttgaca
       61 attatttagt tttaattcta cggttttcac gtaaactttt ttcataaatt gctcattttc
      121 cttataatta tatcacaatc aattttgata tattttacca tttttacgct ttcattttaa
      181 aatcaaactg ttttatttat tacttatacg acataaaaaa gaagcaaaca ccattttgtc
      241 tgcttctttt aaaattaata tccctacgtt attaataact taaagactaa atattaatta
      301 tttagcgcgg ttagaagtac cgaactcttt aattttacct ttaactgttt ctttgatggc
      361 ttcacgtgca ggtcctaagt atttacgagg atcgtaaact tctttgtcgt tatttaaaac
      421 gtcacgaact gcttttgctg aagcgatttg gttttcagtg tttacgttaa ttttagctgt
      481 accaaatgga attgcttttt ggatatcttt agtcgggata ccagtaccac cgtgtaatac
      541 taatggtaaa cctgtagata aaccgatttc ttccatttct ttaaatccta attttggttc
      601 acctttgtat ggaccatgaa ctgaacctaa tgctggcgct aatgcatcaa taccagtttt
      661 ttcaactagt tcttgacatt ctttaggatc agcataaatg atgccgtctg ctacaacatc
      721 atcttcttgt ccaccaacag tacctaattc agcttctaca gaaacacctt tttcatgagc
      781 gtattcaaca acttttttag ttgttgctac gttttcttcg aatgggctgt gtgaagcatc
      841 gatcattact gatgtgaaac cagcatcgat agcttcttta catttttcaa agcttgaacc
      901 atggtctaaa tggattgcta caggaatagt gatgtttaag tcatgcatta acccttcaac
      961 cattttaaca attgtgtaga aaccgctcat gtaacgagca gcaccttcag aaacacctaa
     1021 aattacaggt gcattttctt cttgtgacgc ttctaaaatt gcttgagtga attctaggtt
     1081 attaatattg tattgaccta ccgcataacc attttctttt gcatcaatta acatttcttt
     1141 cattgaaact aaaggcatga aagttcctcc ttgagtgctt acgcacgtat ttttcaaagt
     1201 aattatatca aaaaaatagc gtcaatgcat tgaaatactg cagattaaat gtgtattttt
     1261 tgaaaaatgc gatagttata ctaaatgcaa acgtatacat ttttatacaa tttaagccgt
     1321 tgcataatat gtagtcatta attacaatat tttataagta gattggagga taaagatgtc
     1381 aaaaatctta aacacacaac taataggtat ctttaatagg ctagaaaaac aatctttaga
     1441 aattcaaatg gccgcccaat gtttaattca agcaattggt ggcgaaggtt atgtttatgt
     1501 aaaaggttat gatgatttac agtttttcga aagtttcatt ctacatagtg atgagcgttt
     1561 aaaatctagc cgaaaacttg acgctattaa agactttaaa gaaattgact caactgatcg
     1621 tgtattatta ttcgcaccat tttataatga tcaagttgca ttagatattc aaaaacttat
     1681 cgacttagat atcgatgtgg tcttaataag caacaaacct aaaacagatg atttccctga
     1741 tcatctcgta cattttatcg atttatctac accaagacct atagtctaca ctgaagatta
     1801 cgataagatt gtccaaccac atgcgatggc attgaattat gtatactatg acatttatac
     1861 acaaatgatt gaaatgacaa gagacttaga attataaaag tcactattgt tatttcatac
     1921 cttgtcacta acataatatt a
//
LOCUS       DAKTIP010000304         1925 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.309, whole genome shotgun sequence.
ACCESSION   DAKTIP010000304 DAKTIP010000000
VERSION     DAKTIP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.309"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            51..1361
                     /gene="sbi"
                     /locus_tag="O0305_002752"
     CDS             51..1361
                     /gene="sbi"
                     /locus_tag="O0305_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein Sbi"
                     /protein_id="HCY0275843.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     SKLNEKDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKVESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYYNTYYKYKGAIDQTVLTVLGSGSKSYIQPLKVDDKNGYLAKSYAQVRNYVT
                     ESINTGKVLYTFYQNPTLVKTAIKAQETASSIKNTLSNLLSFWK"
BASE COUNT          813 a          340 c          251 g          521 t
ORIGIN      
        1 attctaaaag catacaataa atttaatatg taaaaagaaa gggaatacac atgaaaaata
       61 aatatatctc gaagttgcta gttggggcag caacaattac gttagctaca atgatttcaa
      121 atggggaagc aaaagcgagt gaaaacacgc aacaaacttc aactaagcac caaacaactc
      181 aaaacaacta cgtaacagat caacaaaaag ctttttatca agtattacat ctaaaaggta
      241 tcacagaaga acaacgtaac caatacatca aaacattacg cgaacaccca gaacgtgcac
      301 aagaagtatt ctctgaatca cttaaagaca gcaagaaccc agaccgacgt gttgcacaac
      361 aaaacgcttt ttacaatgtt cttaaaaatg ataacttaac tgaacaagaa aaaaataatt
      421 acattgcaca aattaaagaa aaccctgata gaagccaaca agtttgggta gaatcagtac
      481 aatcttctaa agctaaagaa cgtcaaaata ttgaaaatgc ggataaagca attaaagatt
      541 tccaagataa caaagcacca cacgataaat cagcagcata tgaagctaac tcaaaattac
      601 ctaaagattt acgtgataaa aacaaccgct ttgtagaaaa agtttcaatt gaaaaagcaa
      661 tcgttcgtca tgatgagcgt gtgaaatcag caaatgatgc aatctcaaaa ttaaatgaaa
      721 aagattcaat tgaaaacaga cgtttagcac aacgtgaagt taacaaagca cctatggatg
      781 taaaagagca tttacagaaa caattagacg cattagttgc tcaaaaagat gctgaaaaga
      841 aagtggcgcc aaaagttgag gctcctcaaa ttcaatcacc acaaattgaa aaacctaaag
      901 tagaatcacc aaaagttgaa gtccctcaaa ttcaatcacc aaaagttgag gttcctcaat
      961 ctaaattatt aggttactac caatcattaa aagattcatt taactatggt tacaagtatt
     1021 taacagatac ttataaaagc tataaagaaa aatatgatac agcaaagtac tactataata
     1081 cgtactataa atacaaaggt gcgattgatc aaacagtatt aacagtacta ggtagtggtt
     1141 ctaaatctta catccaacca ttgaaagttg atgataaaaa cggctactta gctaaatcat
     1201 atgcacaagt aagaaactat gtaactgagt caatcaatac tggtaaagta ttatatactt
     1261 tctaccaaaa cccaacatta gtaaaaacag ctattaaagc tcaagaaact gcatcatcaa
     1321 tcaaaaatac attaagtaat ttattatcat tctggaaata atcaatcaaa aatatcttct
     1381 ctagttttac atcatttttt aaataatttt cgtaacaaac cgtgattaaa aagaaccgtt
     1441 gattctcaat cgaatctacg gttctttttt cattttccat caattaaatg cttcttcgct
     1501 atttgtcagc ccactttttt acctgcaact tgttaaataa tccttacatc gttaacgaat
     1561 agttcatcat ttagttgaat cagctcaact ttattaactt catattttca caaactattg
     1621 cgcaatccat tccttttcca ctacaagcac cataattaaa caacaattca ataaaataag
     1681 acttgcaaag catagttatg tagctatata aacgcctgcg accaataaat cttttaaaca
     1741 taacataatg caaaaacatc atttaacaat gctaaaaatg tctcttcaat acatgttgat
     1801 agtaattaac ttttaacgaa cagttaattc gaaaacgctt acaaatggat tattatatat
     1861 atgaacttaa aattaaatag aaagaaagtg atttctatga ttaaaaataa aatattaaca
     1921 gcaac
//
LOCUS       DAKTIP010000305         1924 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.310, whole genome shotgun sequence.
ACCESSION   DAKTIP010000305 DAKTIP010000000
VERSION     DAKTIP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.310"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(287..>1924)
                     /locus_tag="O0305_002753"
     CDS             complement(287..>1924)
                     /locus_tag="O0305_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCY0275844.1"
                     /translation="SSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTNNIK
                     MWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFSAGM
                     GIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALALAY
                     SQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQINGGL
                     HYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAALIVG
                     PTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPFVGV
                     FIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQLFG
                     VFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGISQA
                     LIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLGLTL
                     TPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          691 a          378 c          286 g          569 t
ORIGIN      
        1 attgatttat gtcccagcct gagttaattt tctataaaag tatatttaat ttgcgtttat
       61 accgtcaaac ttcactttag ctttgtcaaa cccctttcta ttaagttttc agaaataaac
      121 ctatcttaaa atataaaaaa atcgagaatt cgtagtttaa taacgaaatt ctcgttctta
      181 tccttttgaa tatactcaat tttccacaaa aacaaacaag tagtatatct gttctagcta
      241 ctagaatgac atactacttg ttattaaaat acttaactaa actttattag ttatcttttt
      301 tctctatatt tctacgtgac tgacgctttt caagaatgtc agattcataa tcttcttgtt
      361 gactcttgat atattcttgt aagcgatgtt tattcggagt caatgttaaa cctaggaatt
      421 tacgttcctg gttcgcatcc ttgtagaaac ttaccatcat gagtatgacg acaaaggaga
      481 atgggaatgc acttataatt gcagcacttt gaatcgcatt taaagcttca gcgccgttac
      541 cgccaccagc taataaaagt acaaatgcta ttaaggcctg tgaaattccc caaacaactt
      601 ttaccatact agatggattt aatgaaccaa atgttgtttg cattcctaat acaaatgttg
      661 ctgagtcagc agatgtaata aagaatgatg caattaataa taatgcaatc aacgataaaa
      721 caatgccaaa tggcacatga ttaaacactc caaatagctg tgtttcagga gtcatatcaa
      781 aaatttcttt gtgtttctta cctgtctcga tgcctaatac accaaagaca ctaaaccaaa
      841 caaaactaac aattgctgga actagcaaga caccagaaat gaactctcta attgaacgtc
      901 cttttgaaac tcgtgcaata aacactccaa cgaatggact ccaacttaac caccaacccc
      961 agtaataaag tgtccatgaa gacatccatt cacgtttttg aggatttaaa gctgctgtat
     1021 caaaactatt aaacaagaat gtgtttagta aactacccgt agagctagtt aacatattta
     1081 aaataagaac agttggtcca acaattaaag cagctaccat taaaatagta cctaaaccaa
     1141 tgttcaagtt acttaagtat tgaatacctt tacttaatcc agaccatgca cttgctataa
     1201 ataagatagt aacaacaatg atgataatcg cttgtacaaa cgtattgttt ggaacattga
     1261 acaagtaatg taaaccacca ttaatttgta gagcacccat acctaacgaa acggctaccc
     1321 caacgattgt cgcaaataca gataaaacgt caataaaaat cccaatagga ccttctactt
     1381 tatcacctaa aagaggacgt aaagttctag ataataaacc tggttcacct ttacggaatt
     1441 gcgaatatgc caacgctaac gcaacaacac cataaacagc ccaagcatgg aatccccaat
     1501 ggaaaaatgt tgaacgtaga gcttcagtat aagcttcagt agttttggga tctgctgtag
     1561 gtggcgtagc aaagtgcgcc atcggttcag ctgcaccata aaacaccaaa cctatcccca
     1621 taccagcact aaacaacata gcaaaccatg aaattgtatt aaactcaggt ttgtcatttg
     1681 gtttacctag tttaagtttt ccaataggac taaaaataag gaatatacag aagaacacga
     1741 taatcgtagt aagaataaga taataccaac ctaacttttc tgtaatccac attttaatat
     1801 tattggtaac atagttgaat tgttcaggta aaaatgcacc aagtaatacg actatagcaa
     1861 caacaattgc actatagatg aagactggtg aatacttctt tccatttgga ttctctggtg
     1921 aaga
//
LOCUS       DAKTIP010000306         1908 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.311, whole genome shotgun sequence.
ACCESSION   DAKTIP010000306 DAKTIP010000000
VERSION     DAKTIP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.311"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..613
                     /locus_tag="O0305_002754"
     CDS             <1..613
                     /locus_tag="O0305_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCY0275845.1"
                     /translation="ASKNKKLVRLNYKSEAYVGVWTEESVAVSFLTSRDIPFDKVVKM
                     DVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAVTQEVMTELDKIRIKEFVQDV
                     AKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSIKNRALKVRDEDFEECDLITI
                     EGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLSNELTF"
     gene            complement(743..1546)
                     /gene="coaW"
                     /locus_tag="O0305_002755"
     CDS             complement(743..1546)
                     /gene="coaW"
                     /locus_tag="O0305_002755"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCY0275846.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLEK"
BASE COUNT          667 a          286 c          345 g          610 t
ORIGIN      
        1 cgcctctaaa aataaaaaac tagtacgcct aaattataaa agtgaagcgt atgtaggcgt
       61 ttggacagaa gaaagtgtgg ccgtatcatt tttaacaagt cgtgatattc catttgataa
      121 agttgtaaaa atggacgttg atcgttttgc tacttatgaa ttagatgaat tgtttgatga
      181 acaagaccat attattatga atcaaacaat ggaagaagaa gggcatctac taaacgttgt
      241 agctgttaca caagaagtga tgacggaatt agataaaatt agaatcaaag aatttgtcca
      301 agatgtagcg aaatatgatg aagtatacgg cttaactaaa aaaggtagta agcagtttat
      361 tctcattagt gaaaatgata gcgacgaaaa aaagccgcat attatgcctg tatggagtat
      421 taaaaacaga gcgttaaaag ttcgagatga agattttgaa gagtgtgatt taattacgat
      481 tgaaggttct gttttcggag aatggctaga tgaacttaga gatgatcata aagccgttgc
      541 gatagattta aaaactggcg tggttggtac aattgtttca gcgcaaaaat tgtctaatga
      601 acttacattt taattgaatt gagttgtaag taattaagtg ataataaatg gaacaatacg
      661 tattcaaaac acttagacca taaaataaaa ggccatttat atagcgttta tttaaaacaa
      721 cgcgcatata aatggtcttt ttctattttt ctaaatataa tgcaccaata gcacctgaaa
      781 atgcgccgtt ttcaacatag tacggtttgc aaccgcgtaa cacagtataa tcttccacaa
      841 ctttgcgtaa taaagcgtta ttatgaaatg aagaaccgat ataaacgata ttttcagttt
      901 taaattcacg tgcaacagta atggccattg tcgtaacaac ttcgccaacg acaccaataa
      961 cggctgctaa tttattgcta ggtgtaaaat cagcatctaa atgatgtagt acatgaccaa
     1021 aattagctgc tgttaaatca ccgggaatgg gtggttcggt atctttataa atatgtctaa
     1081 cctttaaatc gatagtgtta cgatcaccgt gttgtgccat gtcagttaac tgtttataat
     1141 cagtgatttg acttagtaaa taaccgagtc cttgaatcat gcctccacct gtaccgatac
     1201 cgcctacacg acgttgtgat tggccgtcga aataatgtag tgacgtaccg gtaccaacat
     1261 ttgcaaaaat ataatctgct aagtcatggc cttgctcttt taacaaaata cctagtcctt
     1321 gagatgcagc atcaaactct acaaaaattt gtgcaggaat gttgatgttt tcagcaatga
     1381 cacctgcatt acctccagtt aagcataatt tttcaatttg ctgttggttt aaccattcca
     1441 caacttgatc aatattttta gttaattcag ttttaaaagt acgttggtta tcttgctctt
     1501 gaacgatttt aattagtgta ccgccagcgt caatgccaac tttcataaga ttcccacctc
     1561 attattaatg tctatcctta aataatagta tagtaaaatg actaaaaaac aagtaataat
     1621 agtaattatt aacaaatttg atgccattgc atttcaacat tgtaagcgta tcgcaattaa
     1681 tgttttacaa acgtggacgt taagttatat atattatttt ctaggaattt tgaagttgta
     1741 taggattgtt agttagtgac gcaatattaa aagtagttcg tacgcagtgt atttgtaagt
     1801 ctctgattaa aatgataagt aatgaggaat agtacattaa ttttgaaatt taaaaaatat
     1861 aaataagtaa tttatttaac ttagagcaaa taatggtatc gtagtgaa
//
LOCUS       DAKTIP010000307         1846 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.312, whole genome shotgun sequence.
ACCESSION   DAKTIP010000307 DAKTIP010000000
VERSION     DAKTIP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.312"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..926
                     /locus_tag="O0305_002756"
     CDS             <1..926
                     /locus_tag="O0305_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCY0275847.1"
                     /translation="ISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFF
                     YSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTL
                     KIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLN
                     GIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDNAI
                     EASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLST
                     LKEIADNADNVLLDTIIENGFFIQKVEIINN"
     gene            945..1661
                     /locus_tag="O0305_002757"
     CDS             945..1661
                     /locus_tag="O0305_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCY0275848.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIVHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            1733..1840
                     /locus_tag="O0305_002758"
     CDS             1733..1840
                     /locus_tag="O0305_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275849.1"
                     /translation="MHCIAGFLILLTKLNMTRELKNDVTSLCIPIPITT"
BASE COUNT          675 a          259 c          287 g          625 t
ORIGIN      
        1 ctattagtat actattctca tatataataa atagagttct caaaaaaatt agcacaccat
       61 atctaatact aaacaaagga tttttaatag ttatttcgac tatcttactg cttacttttt
      121 cattattttt cttttattca caaataaact cggatgaagc taaagtaata aggcagtatt
      181 cttttatttt tattggtatc actatatttt taagtatatt aacatttgtt atttctcaat
      241 ttctccttaa agagatgaaa tataaacgta atcaagaaga aattgaaacc tattatgaat
      301 atacattgaa gattgaagct atcaacaacg aaatgcgcaa gttccgtcat gattatgtca
      361 atatcttaac gacactttca gaatacattc gagaagatga catgcctggc ctacgtgatt
      421 atttcaataa aaatattgta cctatgaaag acaatttaca aatgaatgct ataaaattaa
      481 atggtatcga gaatcttaaa gtacgtgaaa ttaaaggctt aattactgcg aaaattttac
      541 gtgcacaaga aatgaatttt ccgattagta tcgaaatacc cgatgaagta agtagcatta
      601 acttgaatat gatcgattta agtcgcagta ttggtattat tcttgataat gcaattgagg
      661 catcaactga aattgatgac cctatcattc gcgttgcatt tattgaaagt gaaaattcag
      721 taacgtttat tgttatgaat aaatgcgctg atgatatacc acgcattcat gaattgttcc
      781 aagaaagttt ttctactaaa ggtgaaggtc gtggtttagg tctatcaact ttaaaagaaa
      841 ttgctgataa tgcagacaat gtcttattag atacaattat cgaaaatggt ttctttattc
      901 aaaaagttga aattattaac aactagccat aaggatgtga atgtatgaaa attttcattt
      961 gcgaagacga tccaaaacaa agagaaaaca tggttaccat tattaaaaat tatataatga
     1021 tagaagaaaa gcctatggaa attgccctcg caactgataa tccttatgag gtgcttgagc
     1081 aagctaaaaa tatgaatgac ataggctgtt actttttaga tattcaactt tcaactgata
     1141 ttaatggtat caaattaggc agtgaaattc gtaagcatga cccagttggt aacattattt
     1201 tcgttacgag tcacagtgaa cttacctatt taacatttgt ctacaaagtt gcagcgatgg
     1261 attttatttt taaagatgat ccagctgaat taagaactcg aattatagac tgtttagaaa
     1321 ctgcacatac acgcttacaa ttgttgtcta aagataatag cgttgaaacg attgaattaa
     1381 aacgtggcag taattcagtg tatgttcaat atgatgatat tatgtttttt gaatcatcaa
     1441 caaaatctca cagactcatt gtccatttag ataaccgtca aattgaattt tatggtaatt
     1501 taaaagaact gagtcaatta gatgatcgtt tctttagatg tcataatagc tttgtcgtca
     1561 atcgccataa tattgaatct atagattcga aagagcgaat tgtctatttt aaaaataaag
     1621 aacactgcta tgcatcggtg agaaacgtta aaaaaatata ataagataat aaagtcagtt
     1681 aacggcgtat tcaattgtaa atcttgttgg attttaacaa gataactagc aaatgcactg
     1741 tatagctggc tttttaattt tattaacaaa attaaatatg acgcgtgaat taaaaaatga
     1801 tgtaacgtct ctttgtatac ctattcctat tactacataa tttatc
//
LOCUS       DAKTIP010000308         1845 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.313, whole genome shotgun sequence.
ACCESSION   DAKTIP010000308 DAKTIP010000000
VERSION     DAKTIP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.313"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..169
                     /locus_tag="O0305_002759"
     CDS             <1..169
                     /locus_tag="O0305_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0275850.1"
                     /translation="FYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSI
                     KKDIENEGKTM"
     gene            203..790
                     /locus_tag="O0305_002760"
     CDS             203..790
                     /locus_tag="O0305_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0275851.1"
                     /translation="MIIFILITIFAIYYIAMIASLFKSEGFSIIGLILDVVIMGTLIF
                     YYFIGARFVDHDLSNFLMFMDTGSYIFMYFAIKCLWVKPKVVNYLIAKELGESKEVIE
                     EQELDLQTSKIRGIYFFIISVVMLIITKLRMQPELQADAISMNPVFIFVGVIIILIWL
                     VLDIYRKKKYGIFLFKTIVPLVVTTWIIIATIILS"
     gene            complement(933..1826)
                     /locus_tag="O0305_002761"
     CDS             complement(933..1826)
                     /locus_tag="O0305_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0275852.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
BASE COUNT          543 a          251 c          351 g          700 t
ORIGIN      
        1 attttatata agtctaatag ttcctgtaac acagcaagaa gagattttta tagggttaag
       61 tatattcatc aatataattg ttgatgctct tcttgttagg tcatatttta attacgcgtt
      121 atataagagt attaagaaag atatcgaaaa tgaaggtaag acaatgtaaa ggaaagacat
      181 aaaaaataat aggagtctaa ccatgataat ttttatttta ataacaatat ttgctattta
      241 ttatatagct atgattgcta gtttgtttaa aagtgaaggt ttttcaataa taggtttaat
      301 attagatgtt gttataatgg ggacattgat tttttactat tttataggcg ctcgctttgt
      361 tgatcatgat ttaagcaact ttttaatgtt tatggatact ggatcatata tttttatgta
      421 ctttgcaatt aagtgtttat gggtgaaacc taaggtagta aattatttga ttgcaaaaga
      481 attgggtgaa tctaaagagg tcattgaaga gcaggaatta gatttacaga catcaaagat
      541 aagaggtatc tactttttca ttatttcagt agtgatgtta attatcacta aattaagaat
      601 gcaacctgag ttacaggcag atgctatatc gatgaatcct gtatttattt ttgttggtgt
      661 tataatcatt ttaatttggc tagtactgga tatatatcgc aagaaaaaat acggtatatt
      721 cttattcaag acgatagtgc cactagttgt tacgacttgg attattatag ctacaattat
      781 actttcgtaa acgattttga ttaatgggga atgtagagaa catttctttg taattttata
      841 atcgaataac atacaaaata aagcgaccaa ttttgcagtg ttacgaattg taaactgcga
      901 actggtcgct ttttattgat gtcatgatta atttaatgga tgtaattata tgatgaaact
      961 tctgaagcag agatggttct tgatgaaacg atatattcac caatccagtt catttctgaa
     1021 attagaatac ttccatcaat attaactttt tcaacgtagg ctacatgacc aaatggacca
     1081 tttactgttt gtaaaattga tcctcgtgtt gggtgtctat ctactttgaa gccattgctt
     1141 gaagcttggc ctgcccagtt tttagcatct ccccaaaatg tactaatcgt gtgtccatct
     1201 ttggcacgtt tatcaaagac ataccatgta cattgtccag cagtatataa gttgttctta
     1261 cttgtgataa gaggctgatc aatgatttta ccgttaccta atgctaaagg tttaccgtca
     1321 gcggtctttg ctttgtcatt aaattcggcg atttgtaatt cgtcatacaa ttcgtctaaa
     1381 tcgatgcctg taataagccc tttgttatct tttgaaaaag cgttatttaa ttcatcgtca
     1441 ttgtcttcga cattcggtat tgctggtgtc aaaggattgc ttggtgacgt ttgaggcggt
     1501 gtgtgtgaat caattgcgtc attaatgtgc gtatactgac cacttaatga agaatggtac
     1561 tgattgttgt taagatcacg ttgatttgcg tgttgatgat tgtcgtttgt acgtgactgg
     1621 ttttgatgat tgttgtttgg cgtgttgttt ttgtcatatg tataagtata cgcgccggtg
     1681 tctttattca ctttgaactg tgcgtttggg tgtgctttct ttgcttcttc taatgttttg
     1741 ctatcattcg tatatgcttg agccgagtta ggcgacatac taaataaagt aagagttgtc
     1801 atcgtcagta aaattgtttt cttcataata accatttaat ccttt
//
LOCUS       DAKTIP010000309         1843 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.314, whole genome shotgun sequence.
ACCESSION   DAKTIP010000309 DAKTIP010000000
VERSION     DAKTIP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.314"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            66..425
                     /locus_tag="O0305_002762"
                     /pseudo
     CDS             66..425
                     /locus_tag="O0305_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(516..1253)
                     /gene="sdpB"
                     /locus_tag="O0305_002763"
     CDS             complement(516..1253)
                     /gene="sdpB"
                     /locus_tag="O0305_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCY0275853.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            1302..1631
                     /locus_tag="O0305_002764"
     CDS             1302..1631
                     /locus_tag="O0305_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275854.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTDWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINENQVSYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
BASE COUNT          703 a          295 c          224 g          621 t
ORIGIN      
        1 cctcaggagt ctcgccatta atactacgta ttaacatgta attttacttt gaaatactta
       61 aaaaaataaa acactttgcc caacttacac taccaataga aactgctgtt agaattcctc
      121 aaaatgatat ttcgcgatat gttaatgaaa ttgttaaaaa gatagctgat agcgaattcg
      181 atgaattcag acatcatcgt ggcgcaacat cctatcatct aaaaatgatg ttaaaaatca
      241 cctcatattc atatactcaa tctgaatttt ctggccgtag aatagaaaaa ttacttcata
      301 acagtattcg aatgatgtgg ttagctcaag atcaaacacc ttcttataaa actattaatc
      361 tttttagagt gaatcctaat actgatgcgc taattgaatc tttatttatt cagtttcata
      421 ataaaatgca tatcaaaaaa gctgatttct atcaaataat taatagaaat cagctttttt
      481 cattgcctaa aaacttaatg tcccgacctc tttatctacg cataaatact tattactgat
      541 ataacgaaag aaacaaaatt atttgctata tgtaatgcaa ttgttgaacc taggtttctt
      601 ccagatttta aataagtgaa aactaatatg atggatagta tgagatatgg accaaactca
      661 aacggcgact ttgcatcagt cacatgaata aatgcaaata agaacaccga aacaatactc
      721 atagctataa aattaaactt cttacctaat tctccaatta aaatatgtct aaatacgatt
      781 tcttcaacta ttggacctac aatcacaatt aataagaatg ctacaggtaa aaatgcaggc
      841 acttcaaaca ttttatttag ctcaagttca ttggctgttt cactatattg caaatgttta
      901 ggtagaaact gtgtcatata ttcatatgta taaattaaga tgagagcaat aatatacgtt
      961 attgacaatc taagccaata ttttttgata tacgcaaaac cagctcgaag ccttgatggc
     1021 atcactttta aatgaaataa ataaaatgcg ccaatcccaa tcgtatatgc taaagcttgt
     1081 gtgatagtcg ctacaaatat cagattacta tcgatttcat aataaccaaa caaaattggt
     1141 cctatgtaag ctgcaattgt gagtgcataa aatataacac ctataattgg aattataagc
     1201 aaatctctcc atgctatatc tttaaacgtg tatttctttt tttcattttc cactgttata
     1261 tcctttcctg tttaataatt gatttttgga ggtacttcta catgataaac gaaactaagt
     1321 atatgagaca acaaattact aatttgattc aaatcattga tacgattaaa tataatactt
     1381 taatgacaga ttggaacatt caaacgcata tttataaatt taaccaaata gttactaatg
     1441 aattgaataa gttcaaaggc tttgaaacat catatataat aaacgaaaat caagtttcct
     1501 attatgaaat tataacacta cttaataaac gtcccctccg acaagtcgac tatggtaaca
     1561 aaattcaata tcttaatttt tatcatacag aactatctaa cgcattattt gcaattaaat
     1621 ttgcccatta acctattttt cataaaatgt catttaaaca agttatttat taaaattcac
     1681 tttattacat aaattataca attaaaaagt ttcttcaaat tgtaaagatg cattaatcga
     1741 gttataatca taatgattaa gatggtagat ttagtcttat ataactacat ttactaagat
     1801 ttaaatagat gagtacataa tgtataaatt gggaatatac agt
//
LOCUS       DAKTIP010000310         1832 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.315, whole genome shotgun sequence.
ACCESSION   DAKTIP010000310 DAKTIP010000000
VERSION     DAKTIP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.315"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            138..1628
                     /locus_tag="O0305_002765"
     CDS             138..1628
                     /locus_tag="O0305_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HCY0275855.1"
                     /translation="MHRESKYIEYKKSRKGLSNDIWSTYSAFANTEGGTIYLGIEEKK
                     IEDKKVFVSVGVEDPEKMIEDFWNALYGRSKVSQNILSNKDVKIVNIENKACIEIHVP
                     EAPYSKKPIYVDNKKDLVYKRVDDADRIATEEEYKFMIVNSQDDIDTELLDNYDMSDL
                     NHESIENYRKLLLKNTNDERYANMSQLDLMIDLGAYRKDRSSKDKQYKMTTACLLFFG
                     KYNAISDRFPGFQLDYFKKTNYLDTDWKDRISSGDLGNEDLNVYSFFEKVLIKLTDNI
                     EESFSLNDGLTRQNYARDLKVAIREALVNTLMHAYYDTKQSIKIVNCEDFIEFYNPGN
                     MRINKEDFIHGGHSKDRNSILSTLFRRVGYSEKAGSGGPRIFDVVNRHKLKTPEIELT
                     DMDTNVVLWKQDLMKEFEKYPELDKKVIKYIIDYGSISKGEALKMENMTEYQFRNILK
                     KLKDDNLIKKEGEGPATKYVLIESKEADILRTKKVIKSLESFFRNK"
BASE COUNT          742 a          180 c          359 g          551 t
ORIGIN      
        1 cgaaaatcgc gtgaaataat cagaggaatc gtttgaaatc atcgtagtaa tcgcgcgaaa
       61 atcgcgtgaa atactatggt agacggtttg agtaaattaa tggagtattt taatatttat
      121 ggtttggagg aattgcgttg catagagaaa gtaaatatat agagtataag aaatcacgaa
      181 aaggattatc taatgatatt tggtctacgt atagtgcttt tgcaaatact gaaggtggta
      241 ctatatattt aggaattgaa gaaaaaaaga tcgaggacaa aaaagtcttt gtttcagttg
      301 gtgttgaaga tccagagaaa atgattgaag atttttggaa tgcactatat ggaagaagta
      361 aagttagtca aaatatttta tcaaataaag atgttaaaat tgttaatatt gaaaataaag
      421 cgtgcattga aattcatgta ccagaagcgc cttattcgaa gaaaccgata tatgtagata
      481 ataaaaaaga tttagtatat aaaagagttg atgatgctga tagaattgcg actgaagaag
      541 agtataaatt catgattgta aattctcaag acgatataga tacagaatta ttagataact
      601 atgacatgtc tgatttaaat cacgaatcta tcgaaaatta taggaaactt ctattaaaaa
      661 atactaatga tgagagatat gcgaatatga gccaactgga tttaatgata gatttaggag
      721 catatagaaa agatagaagt tcgaaagaca aacagtataa aatgactaca gcatgtttat
      781 tattctttgg taagtataat gcgattagtg atagattccc aggatttcaa ttagattatt
      841 ttaagaaaac aaattaccta gatactgatt ggaaagatag aatatcaagt ggagatttag
      901 gtaatgaaga tttaaacgtg tatagttttt ttgaaaaagt attgataaaa ttaactgata
      961 acattgagga atcatttagc ctaaatgatg gtttgactag acaaaattat gcaagagatt
     1021 taaaagtagc aattcgcgaa gcactggtta atacattaat gcatgcgtat tatgatacta
     1081 agcaaagtat taaaatagtt aattgtgaag attttataga gttttataat ccgggtaata
     1141 tgagaataaa taaagaagat tttattcatg gagggcattc aaaggacaga aatagtatat
     1201 tatcgacgct tttcagaaga gtaggatatt cagaaaaagc tggatctgga ggaccaagga
     1261 tattcgatgt agttaataga cataagctta aaacgcctga aatagaatta acggacatgg
     1321 acactaatgt agtactttgg aaacaagatt taatgaagga gtttgaaaaa tatcctgagt
     1381 tagacaaaaa agtaataaag tatattattg actatggatc aataagtaag ggtgaagcct
     1441 taaaaatgga aaatatgaca gaatatcagt ttagaaatat tttaaaaaaa ctaaaagatg
     1501 ataacttgat aaaaaaagaa ggtgaaggtc cggctactaa atatgtgtta atagaatcaa
     1561 aagaagctga tatattgcga actaaaaaag taattaaaag tttagagtct ttctttagga
     1621 ataaataaaa aaacaagaga taggtgcgaa gtgtttggat tatacaaatg cttcgcatct
     1681 tatatttatt aataaatatc atagaaaacc gtatcattaa ccgatacgca gagatgcggt
     1741 tttttagaca cttcataaag ggattttgaa cgtatcagaa catatgaggt ttataggaat
     1801 tgctgttatg ttttttgatc acatcaataa ac
//
LOCUS       DAKTIP010000311         1831 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.316, whole genome shotgun sequence.
ACCESSION   DAKTIP010000311 DAKTIP010000000
VERSION     DAKTIP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.316"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(67..1704)
                     /locus_tag="O0305_002766"
     CDS             complement(67..1704)
                     /locus_tag="O0305_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCY0275856.1"
                     /translation="MDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRG
                     KFGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANH
                     QITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDAS
                     ELASRYIEEVGDPLQIDIPISKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVV
                     FTSLHGTSVPIVPELLQSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELA
                     NKSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMI
                     QSIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPF
                     VRDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKK
                     INAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEG
                     FIALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
BASE COUNT          597 a          325 c          295 g          614 t
ORIGIN      
        1 ttctagtttc aaaagggctg ataagcataa aatgaaatgt aatatttcga tgtataaaat
       61 ttttaattag ctaaaaatca tcgcattaat tttttgagct acatcatcaa aattcggaca
      121 ttttaacgac acatataatt taattttagg ttcagtacca gaaggacgta aagcgataaa
      181 tccttcgtca aataagacac gaataacatt tgatttagga gaattaatct gcgacgttgt
      241 atctttatcc aaatgataaa cctcgctagt taaataatct tcaattgctt tcactttgag
      301 tccttgaatc tcttgcggtg gatttgaacg gaatttggtc attattgcat taattttctt
      361 tttcccttca aatccttcta gcgtatgcga gaataatgta tcctcatgtc taccaacagt
      421 ttgataaatc tgttctaatt catctttcaa tgttttgcca tataatttta actcagaagc
      481 gtattttata atgagtggca caatttgtac ggcatcttta tcacgtacaa aaggctctga
      541 tagaaaaccg taactctctt caaatgcgaa aatcatattt tgatgatcat ccagttgtct
      601 tatttcctga gcaataaatt taaagcccgt cagcacctct ttgtattcaa cattattata
      661 gcgtgctaat gactttgtta actcactact tacaatagat tgaatcatta agcggtgacg
      721 taattgagac gtttgttgaa tacgataatt aagtaacagc gcaccaattt ggttgccatt
      781 aaaatatgtg atatgaccgt gtgcatcacg ttcagcaata ccaagtcgat ctgcatcagg
      841 gtcagttgat attaataagt ctgcatgact tttatttgca agttcgactg cttgatcaaa
      901 agcgcgatga tcttcaggat tagcactttg aactgagctg aaattcggat caggtttaca
      961 ttgtgcttct acaagattaa attgattgaa attcaaagat tgtaataact caggtacaat
     1021 cggaacactt gtaccatgca aactagtgaa cacaacttgt aaatcagatt taggaatata
     1081 gccaatcata ttttgaatgt gtttcatata gtcatcagtt acagatttcg gaaaaggctt
     1141 gatataagat gtattttgtt tagaaatagg tatatcaatc tgtaatggat cgccaacttc
     1201 ttcgatataa cgacttgcaa gctcagatgc atcagtcgat aattgcgcac catcagaacc
     1261 atatactttg atgccgttat agtctttcgg attatgactt gctgtaatca taatgccagc
     1321 agtagtatta agattgcgca ccgcgaacga taattccggt gtcgttttat atgtatcagg
     1381 taaataaact gttatttgat ggtttgctaa tacattagca attatttggg cgaattcagt
     1441 tgaaagatgt ctaatatcat aatgaatgac tattgttgga ctgtttgttt gggcatttaa
     1501 ataacgcgct aaacctaatg ccaatttttc aatagtaaac ttattaagtc gaccttcacc
     1561 aagaccgaat tttccgcgta tacccgcagt accaaatgtt aatttacttt caaaaccttc
     1621 tcgctgttca atgtcagatt gctgctcata aaaatgttta actaaactat cattagctcg
     1681 ttctatccat aattctttat ccattgttgc taaacatcct ttcaaaatct cagttagact
     1741 taataaaaca tgaaaactaa agcccttaca tttatgtaat gaattataaa gaaatacgcc
     1801 ccaaaagtaa aaaaacacag ccccaagaca a
//
LOCUS       DAKTIP010000312         1823 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.317, whole genome shotgun sequence.
ACCESSION   DAKTIP010000312 DAKTIP010000000
VERSION     DAKTIP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.317"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(118..1137)
                     /locus_tag="O0305_002767"
     CDS             complement(118..1137)
                     /locus_tag="O0305_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCY0275857.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(1211..>1823)
                     /locus_tag="O0305_002768"
     CDS             complement(1211..>1823)
                     /locus_tag="O0305_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0275858.1"
                     /translation="FDSWKSLYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHH
                     LKEAERDLTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPK
                     EGSNLWFDNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKE
                     IKDDHRFYPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK"
BASE COUNT          555 a          340 c          240 g          688 t
ORIGIN      
        1 atttaaaatt atgacaccta aacactttaa ttaaacgtta tcgataatta acctcatata
       61 aaaacccaga ttaataagca aaagacctct gcttactaat ctgggtatca tcaatattta
      121 ttcttgctct tttttgtcct taacttcact tgaattgcgt tcttcagaaa tatcattgtt
      181 gtaaatttga cgtcgctctt caaaattcat tcgacgttga ctaactgcgc taggttgatt
      241 tttcctacgt tctttcattc gttggttata agctttacgt ttttcttttt tcttagcttt
      301 aatttcagct ttttctcgct tttgttgtgc tttcaattct tttggatcaa cttcaggttc
      361 tgcataataa ccatcatctt caatacctag ttcttcagta cttttttgtt tgtatttagc
      421 ctgtctggat aatactgatg gttcttcctc agtgcgctca tcatgattat attcactttc
      481 aactgcacgt ttaggaaatt gatcatattt atctctttcc tgttcttgat catatacagt
      541 atttgattta tccaattctt tttcatattt aggcacatca taataatcat ctttcggttg
      601 ggggcgctca tacacaggtt cacgataatc ataaatcggt ccgttatact cttcataata
      661 ttgtggcgca accatttcaa ctttatcttt tcttataaat agcatcatcg caataataaa
      721 gaataatatc ggaatgatta aagtaacgat aagtaaaact aaaggaatcg ttacaattgc
      781 tgatattaag aataagaaac ctgacaagat tcttatattc atagatatga gagctaaaaa
      841 tgaaatcaac aaacaaacaa taaagtaaac gataatagcc catataccat tttgtaacca
      901 tattacaaat tgtgttgtat tatagccatt tccagctaat aattgctgga taaatgcatt
      961 attgtttaat gtattttcaa gattagcaat cgatgtgtta ttactgaatg aaacaagtgc
     1021 tataaacatc gtaatgacag taagcactaa taacatcacc caacataacc aacctagaac
     1081 tttttcagtt aatctactta ctggacgttt aatttgagta aattgttctc cagtcattcg
     1141 ttacaactcc ttatagtact tatcccgtta ttataactaa atatacagta aataactact
     1201 atttatgatt ttattttaat gacattttga aattcaaaaa gttttcattg tattcactta
     1261 aaacttcagg tcctaaatct ttataaactt caaggcgttc ttgctctttc ttagtcggat
     1321 aaaaacgatg gtcgtcttta atctctttag gcaacaattg tcgagcagcc ttgtttggcg
     1381 ttgcatagcc tacgaattct gtattttgct tgttattttt agcatctaat aaaaaattca
     1441 taaatttata tgcaccctct ttattttgtg ccgtttttgg aattaccata ttgtcgaacc
     1501 ataaattcga tccttcttta ggaataacat aattatattt atccccttct tgcactagag
     1561 gtgctgcaac accactccaa acaaccgcta tgttaccttc attttgttga agcatcatgg
     1621 taatttcatc acctacgaca cctcttactt gtggtgctag tttggttaaa tctcgctctg
     1681 cttcttttaa atggtgcgaa ttacggtcat taagattata cccaagttta ttcaaactca
     1741 tgcctataat ctctctagca ccgtcaacta gtaaaatttg gtttttaaat ttaggattat
     1801 acaatgactt ccaactatca aat
//
LOCUS       DAKTIP010000313         1818 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.318, whole genome shotgun sequence.
ACCESSION   DAKTIP010000313 DAKTIP010000000
VERSION     DAKTIP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.318"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(77..547)
                     /locus_tag="O0305_002769"
     CDS             complement(77..547)
                     /locus_tag="O0305_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCY0275859.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(595..1812)
                     /locus_tag="O0305_002770"
     CDS             complement(595..1812)
                     /locus_tag="O0305_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCY0275860.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT          557 a          288 c          282 g          691 t
ORIGIN      
        1 tcaaaaataa ttagacataa aaaagctata aagatgattg ttgtgaatca tctttatagc
       61 taattaatta ttaatgttat agtgcttctt ttctaataat tgctgtatat tgccaaaaga
      121 ttcgcatttg agcaatattc caattattca ttcccattgt aaatcctgaa ggcttaaata
      181 tattcacatc agttacttct aaagctgctg caactaaata ttgtaatgga atgctaagtg
      241 atttcattgt ttcggtaatg ccattttcat catataccca tgaaaatggt aactctgatt
      301 cgaaaacatc aacactattg aaaatttcag gtagggctaa aatataacat gcaccatcta
      361 caataggatt gtcgatacta tttttgtaat aaattaggag tgcttcatag ttttctcgat
      421 gctcatgatt gacgtaaaaa aattcacttc taaaatgagc catatcttgg ctatgaatga
      481 tttgttgttc taacatatta aacatgccat ttatatttgc aatcttttcg tatgtttttc
      541 tactcatgta tgatgctcct ttcataaata gtaaatgtta atcgtcatat aatattaatt
      601 tacaacacca ttttggttat ttgaagcttg tggcgcttgt tgtgtgccac cttgattttg
      661 atttgagttt tgatctgtag caggttgttg ttgattgctg gaatcactgt tattagttga
      721 atcactgttc tcgttagatg tcttatcttt atctgtcgta tcattttgtt cttttctcaa
      781 taaactatta tctaaaggcg ttaatggtat taatgaacca taatgattaa tgacacgttg
      841 atctaagaaa tcatttttat cattaatagg tgataattct aagtctttac gaagtaagtt
      901 tgcatatttt tgaatgcttt caacacttgg atgataatag taaataccat ttaacatatc
      961 atctttacct tttaattgcg cagttttaat ttcaacatca tttgttaagt acatttttgc
     1021 taaagcttta atttcagagt tagttaaatt atgctttgca tttttaccta caatttgaat
     1081 cacgttatca agtttatcaa tagaatcaac ttcctgtgct ttttggaata aaatcttaat
     1141 taattccatt tgacgttgtc cacgttttaa gtctgaatca tgatgtctag ttctagcaac
     1201 tgctaaagcc tcatcaccat ttaatttttg gtaccctttt ttaattttaa tcttaccagt
     1261 atcatctgtg ttaggttcat ttaagtcgta tggcacatca tagtatatgc caccaagctc
     1321 atttacagcc tcgacaaatg ctttcatatt gactctcaca taataatcaa caggtacatt
     1381 catggtagct tctaccgaat ccattgcggc aattggacca ccatatgcat gtgcatgggt
     1441 aatcttatcg taatagccaa ctttaggaat gtagctgata gtatcacgtg gaatactaag
     1501 cattctaatt tgatgttttg attgattaaa agtagttaaa atcatagcgt ctgatctaga
     1561 gtgttcagca tcctgtcctt tttttcttct tccatcgtta tcatcgatac ctaagaaaag
     1621 aatagagata ggttgttctt cgggattgac tttattatct cttaagttgg attgacgatt
     1681 agcatttttg ctgtcttgag aagattcgaa tgcatcttgg gacgttttaa aaagtaacgt
     1741 agcgaagact attggaacaa caatgagaac caatgctaga aggatcaaaa agtattttaa
     1801 aaatttattc atgattga
//
LOCUS       DAKTIP010000314         1809 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.319, whole genome shotgun sequence.
ACCESSION   DAKTIP010000314 DAKTIP010000000
VERSION     DAKTIP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.319"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            27..>1809
                     /locus_tag="O0305_002771"
     CDS             27..>1809
                     /locus_tag="O0305_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275861.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINNIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHQAFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYEN"
BASE COUNT          721 a          226 c          268 g          594 t
ORIGIN      
        1 acttaaatat agaaagaggt aaagttatgg caaaatcatg cttgcatata cttactaata
       61 atgaatatgc gacaacgcgt tgccaagatg gcatagtctt attttggcca attgacgggg
      121 aaatcgaact acaaaaattt cgtaaaagta aaataattga agatgatata tatattatta
      181 atcatctgga tgtatttagt attaagaata ataaaaaaac gatcatgttg tatttgagta
      241 gcgattggtt tgcggaatta ggctttactt tctttaatta ccactataca gcaaagttga
      301 ttaaatcatc ctataatttg aaatgtctac tattaaaatt gacatatcga taccttgata
      361 atcagcctct taatgacgct gatattagaa aattacagga tattattaaa atcattgcaa
      421 aagaagcaag tatggataaa aagattgcac aaaatcaata tcgatatgcg tattatggtg
      481 atttgcgtga tgagctcgaa tatatttatc aaaatgtaaa tcaacgattg acattaaaaa
      541 gtgtcgctga taaattattt gtctcaaagt caaatttgtc atcacaattc cacttactta
      601 tgggcatggg ttttaaaaaa tatattgata ctttgaaaat tggtaaatcg attgaaattc
      661 tacttactac tgatagtact attagcaaca taagtgaaca tttaggtttt agtagtagct
      721 ccacttactc taaaatgttt aaaagttata tggatataac accgaatgaa tatcgtaatt
      781 tatcaaaata taataaatgt ttaatgctaa agccagaacc actagtaggc aaaatggtgc
      841 aagaagtaaa agaaatcata ttgaattata ttgaacatta taaaaaccac ctaactgatg
      901 ttatacatat tgatgaagac aaatttgaaa cacctaaatt gtttcaaacg gttattcaaa
      961 taaatactta tacagaaatg aaattagttt tcttagaagg aatctttaaa accttattga
     1021 ataagaacag tcaagttgtc tttttcatca tgccatcgat tctaaaaagt aaaaatacca
     1081 tgtccgaaga agaaaaattc acaatcatta aaacaataat tgaaagtgat ctaaagatag
     1141 catttaatat aaataatatt gaaacaactt attttgttga agaagctttt atgagtgttt
     1201 tcagacaaat atctccaaac gaattaagta atcataataa ttacgaagtg cattttgttt
     1261 ttgatttatc attgatggaa attagaacaa tttatcgaat gatattaaaa ttacataaca
     1321 tcatgttgaa tgtgaaatta ggattgaaca ttacctgttt atttgaaaaa ccttcagttt
     1381 ttaaatcact agtatcacaa ataaagcgac ttaaattcga ttcgttaata atagataatg
     1441 caaatttaag tagcccttat ttgatggggg aaagtgatga gttactattg aaaaatattt
     1501 tgcattttaa aaatttaaaa caagtaatta atgaattgga tattgaacaa gaaaagctga
     1561 tttttctaaa tgttgaaaat cataaactgc ttaataataa agaacgagat ctaagtaata
     1621 gtgctccatt aatttataag acattaagtg cgctgtatca caactttgat ggctttggat
     1681 taaacatttt tgacaatcat caagcattta atgcgatgca tctatatgac aaaaatggat
     1741 ttaaaacaac actaggtctt atattagaaa agtttatcga atatgtctcg aaaccaaaat
     1801 acgaaaaca
//
LOCUS       DAKTIP010000315         1805 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.320, whole genome shotgun sequence.
ACCESSION   DAKTIP010000315 DAKTIP010000000
VERSION     DAKTIP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.320"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(74..1165)
                     /locus_tag="O0305_002772"
     CDS             complement(74..1165)
                     /locus_tag="O0305_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCY0275862.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLADLYNAKKLK"
     gene            complement(1626..1730)
                     /locus_tag="O0305_002773"
     CDS             complement(1626..1730)
                     /locus_tag="O0305_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275863.1"
                     /translation="MRSFHVFMLFETSLDNNSDIAHFKLKMEKYSIIK"
BASE COUNT          580 a          319 c          230 g          676 t
ORIGIN      
        1 attgtcgatt caaagataat attctacttc aacatgacat attagttaag tagtctatag
       61 tttccttgga atattatttt aactttttag cgttatataa atcagctaaa ggctttaatt
      121 catcataaaa tgcttggaat tcatctaaat ccatttgttg acccgcatca ctaagtgcaa
      181 cagatggatc tggatgcacc tcagccataa ctccatcagc accaactgct aatgctgctt
      241 tcgcagttgg taacatgata tctttacgac ctgtactatg cgtaacatct accatgactg
      301 gtaagtgtgt accttgtttt aaaattggta ctgctgaaat atctaaagtg ttacgtgtcg
      361 ccttttcata agttcggatt ccacgttcac ataaaataat gttttgatta ccttgtgaag
      421 caatgtattc agctgcataa acaaactctt cgattgtagc agataaacca cgttttaata
      481 gaataggctt tttcgtacgg ccagcttctt ttaataactc gaagttttgc atattacgtg
      541 caccaatttg gaatacgtct aaatactcat cagccacttc aaaatcattt ggatttacga
      601 tttcgctgac aacatttaaa tcatatttat ctttaatctg tttaagtatt ttaagtcctt
      661 caacacctag gccttggaaa tcatatggtg atgtacgtgg tttaaatgca ccgccacgaa
      721 taaatttttc acctttagca tgtaagtttt tagcaacagc ttcaacttgt tcaaatgatt
      781 caactgaaca tggcccaaat acaaatgatt tattgccgtc tccaataatg cccccattat
      841 caaatgttac aatcgtatct tcaggtttca acttacgtga tacatataaa tgtttttcat
      901 tttcagattt ttgtaaatct gtagaggctt tgaaaatttc tttaaataat tgcttaatag
      961 tattatcgtt gaatggtcct ttgttactat cgattaagtc gttaagcatt tctttttcac
     1021 gttgtggatc ataaatacgt gtaccttgtt ttaatttttc ttccccaatt ttttgtgcta
     1081 gttcaccacg tttagataat aagtctaaaa tttgatgatt cagtgataca atctcacttc
     1141 tgtatgattc taatttatta ctcatcctta ctcacctcta ctaattattt caagtatatt
     1201 ccactttaaa gcaataaaat ataatgttaa aaaatattct aacaatatca tatgtatagt
     1261 tcaaccctaa attgacagaa aataatcaat tttctaatta tatgattcga gactgacatt
     1321 tcgattttct cagtcattat tggaaagtta tattctatat tgatatttat tagcaaatgt
     1381 taatcaatta aataagtgtc attattatag cttattttga gtcaacatta ctgcttttaa
     1441 gaaaaatttt cgtttctatc ttctattata aatctaaaat tctcatacat tcaacctaac
     1501 gcttaataaa aattaacaac ctttgtcttt gtatattaat ataaatctat agtgaacatc
     1561 actcaccaac acattcagct atatttaaat tagtgaacgt cattgtacct tctaaaattc
     1621 attgtttatt tgattatcga atatttttcc atcttcagct taaaatgtgc aatatcacta
     1681 ttgttatcta acgatgtttc aaacaacata aatacatgaa atgatcgcat tgcctttcaa
     1741 aataaactga tgatatcaaa aaaggtaagc acctgaatgc ctacctttta ttttgaaatt
     1801 caatt
//
LOCUS       DAKTIP010000316         1804 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.321, whole genome shotgun sequence.
ACCESSION   DAKTIP010000316 DAKTIP010000000
VERSION     DAKTIP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.321"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..180
                     /locus_tag="O0305_002774"
     CDS             <1..180
                     /locus_tag="O0305_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCY0275864.1"
                     /translation="YIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLA
                     LDKAVRDDIYRYLTN"
     gene            486..1259
                     /locus_tag="O0305_002775"
     CDS             486..1259
                     /locus_tag="O0305_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0275865.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
     gene            1371..1804
                     /locus_tag="O0305_002776"
                     /pseudo
     CDS             1371..1804
                     /locus_tag="O0305_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
BASE COUNT          744 a          202 c          327 g          531 t
ORIGIN      
        1 tatatttttg tacagaaaaa agatgatgat ggtatatatt tttattattt aggaactgct
       61 ggatatatag aaggttcaga aaagcaagat aaaatgccga atggttcaaa cgtagtaacg
      121 atggatttag cactggataa agctgtaaga gatgatatat atcgttatct aacaaattaa
      181 tgtaaaccga aaaatgaatg ttagtaaagt tgtatttaaa catcaatttt aataagtaaa
      241 tacattgagt ttcagtgtta ttattttaaa tataagcgca atttatctta attaattagt
      301 tggttaggcc aataaaaatt ataaagatat ttgcgctttt taaataattt gtgatgttga
      361 tatgtgaaaa atacaactaa caattaaaat ccacgaggat ttaatcataa aatttcctct
      421 aatattatta atattggaat ttgaacttaa ctttgcatca aaaataaaaa tggggatgtg
      481 aaatgatgat tcactcaaaa aggttgaggc tctggctgta tttagtatta ttagctgttt
      541 ttataggggc ttgcggaatg aaaaaggaag aaagcagtaa agataaacaa attaaagaaa
      601 acttcaacaa aacgttgagc ctgtatccaa ctaaaaatct tgaagacttt tatgataagg
      661 aaggctttcg agatcaagag tttgaaaagg gcgacaaagg cacttggata attcactcta
      721 aaatgacaat agaaacaaat ggaaagaata tggaatccag aggattggtc ctgtatgtag
      781 accgcaatac cagaacgaca aaaggtgaat ttattgtgag ggaattatgg gaagataaaa
      841 aaggatactc acgtagtaaa gaaaaagaat atccagtaaa aatggaacat aataaaatta
      901 tcccaaccaa accgatagct gatgataaat taagaaaaga aatcgaaaac tttaaattct
      961 ttgtacaata cggtgacttt aaagatatta atgattataa agatggtgac atttcatata
     1021 atccgaatgt gccaagttat tcagcaaagt atcaattaag taatgatgac tacaatgtga
     1081 agcaacttag aaagaggtat gatataccaa ctaagaaagc acctaaatta ttaataaaag
     1141 gcgatggtga tttaaaagga tcatctatag gacacaaaaa tttagagttt acatttatag
     1201 aaaataaaga agaaaacatc tattttacgg atagtattaa tttcaaacct actgaatagg
     1261 gttaatagaa gtttgaaatg aaaatttcat aaagtgttca aaataatttt tcgctttagt
     1321 tgtgtaaatt aaagattgag tcagtattaa aaatataagg tggagaaatg atgattcatt
     1381 caaagaaatt aacgctcggt atatgtttgg tattactcat tatattgatt ggaggttgtg
     1441 tcattatgac aaaaacaaat ggtcgaaacg ctcaaattaa agaaaatttc aacaaaacat
     1501 taagtgtata tccaaccaag aatctcgatg atttttacga taaggaaggt tttcgagatc
     1561 aagaatttga taaaagagat aaagggactt ggattattta ttctgaaatg gttatcgaac
     1621 caaaagggaa aaatatggaa tcgagaggaa tggtgctcta tatcaatcgc aatactagaa
     1681 caacgaaggg taattttatt gtcaccgaaa taactgaaga tagtaaagga tattcacgta
     1741 gtaaagaaaa aaatatcctg tcaagatgga aaataatcga attattccaa caaagcctat
     1801 accg
//
LOCUS       DAKTIP010000317         1781 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.322, whole genome shotgun sequence.
ACCESSION   DAKTIP010000317 DAKTIP010000000
VERSION     DAKTIP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.322"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            71..1781
                     /gene="sasG"
                     /locus_tag="O0305_002777"
                     /pseudo
     CDS             71..1781
                     /gene="sasG"
                     /locus_tag="O0305_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
BASE COUNT          733 a          246 c          366 g          436 t
ORIGIN      
        1 attgtatttg taaaattaca tgtaaattca ctgtaagcaa agtggaaaat atggaaaaag
       61 gagtatgcaa atgagagata agaaaggacc ggtaaataaa agagtagatt ttctatcaaa
      121 taaattgaat aaatattcaa taagaaaatt tacagttgga acagcatcta ttttaattgg
      181 ctcactaatg tatttgggaa ctcaacaaga ggcagaagca gctgaaaaca atattgagaa
      241 tccaactaca ttaaaagata atgtccaatc aaaagaagtg aagattgaag aagtaacaaa
      301 caaagacact gcaccacagg gtgtagaagc taaatctgaa gtaacttcaa acaaagacac
      361 aatcgaacat gaaccatcag taaaagctga agatatatca aaaaaggagg atacaccaaa
      421 agaagtagct gatgttgctg aagttcagcc gaaatcgtca gtcactcata acgcagagac
      481 acctaaggtt agaaaagctc gttctgttga tgaaggctct tttgatatta caagagattc
      541 taaaaatgta gttgaatcta ccccaattac aattcaaggt aaagaacatt ttgaaggtta
      601 cggaagtgtt gatatacaaa aaaaaccaac agatttaggg gtatcagagg taaccaggtt
      661 taatgttggt aatgaaagta atggtttgat aggagcttta caattaaaaa ataaaataga
      721 ttttagtaag gatttcaatt ttaaagttag agtggcaaat aaccatcaat caaataccac
      781 aggtgctgat ggttgggggt tcttatttag taaaggaaat gcagaagaat atttaactaa
      841 tggtggaatc cttggggata aaggtctggt aaattcaggc ggatttaaaa ttgatactgg
      901 atacatttat acaagttcca tggacaaaac tgaaaagcaa gctggacaag gttatagagg
      961 atacggagct tttgtgaaaa atgacagttc tggtaattca caaatggttg gagaaaatat
     1021 tgataaatca aaaactaatt ttttaaacta tgcggacaat tcaactaata catcagatgg
     1081 aaagtttcat gggcaacgtt taaatgatgt catcttaact tatgttgctt caactggtaa
     1141 aatgagagca gaatatgctg gtaaaacttg ggagacttca ataacagatt taggtttatc
     1201 taaaaatcag gcatataatt tcttaattac atctagtcaa agatggggcc ttaatcaagg
     1261 gataaatgca aatggctgga tgagaactga cttgaaaggt tcagagttta cttttacacc
     1321 agaagcgcca aaaacaataa cagaattaga aaaaaagttg aagagattcc attcaagaaa
     1381 gaacgtaaat ttaatccgga tttagcacca gggacagaaa aagtaacaag agaaggacaa
     1441 aaaggtgaga agacaataac gacaccaaca ctaaaaaatc cattaactgg agtaattatt
     1501 agtaaaggtg aaccaaaaga agagattaca aaagatccga ttaatgaatt aacagaatac
     1561 ggacctgaaa caatagcgcc aggtcatcga gacgaatttg atccgaagtt accaacagga
     1621 gagaaagagg aagttccagg taaaccagga attaagaatc cagaaacagg agacgtagtt
     1681 agaccgccgg tcgatagcgt aacaaaatat ggacctgtaa aaggagactc gattgtagaa
     1741 aaagaagaga ttccattcga gaaagaacgt aaatttaatc c
//
LOCUS       DAKTIP010000318         1775 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.323, whole genome shotgun sequence.
ACCESSION   DAKTIP010000318 DAKTIP010000000
VERSION     DAKTIP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.323"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            73..828
                     /locus_tag="O0305_002778"
     CDS             73..828
                     /locus_tag="O0305_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCY0275866.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(1020..1745)
                     /gene="sasD"
                     /locus_tag="O0305_002779"
     CDS             complement(1020..1745)
                     /gene="sasD"
                     /locus_tag="O0305_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCY0275867.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMQDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGVNHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMHSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTNK"
BASE COUNT          550 a          306 c          303 g          616 t
ORIGIN      
        1 ctatatttaa tttttataat ttttccaatt tgagtaaaaa ttattgtatg atgagagtga
       61 ttaagtaact ttatggaggt tgtctacgtg acgcaaacac aaccaaaatt tttaacgatt
      121 tataatacgt tatataaaga aattcaaata ggaaaatatc cgagtggcca tgcattacct
      181 actgaaaagg aactttgtgc gcgctttgat gtcagtcgta tgactttgcg ccaagctata
      241 aaattgttag ccgaagatgg catcgtagag agtacaagag ggaagggaca ctttgttatt
      301 ccacaaacaa atgcccaaca cgcttcaagt atggatcaat ttaagcatcc acttgatcaa
      361 atattgctag ctcgcgtatc tatgtcatca attaattatc gtgttgattt agaaagtgaa
      421 tatacaaatc atttgtttgc aaaccatcct tctgccgtca ttgcaatgga acgctattat
      481 caaaaaaagg acaatcattc taagcaagct gatgcatttt gctttacctt tataccgtta
      541 aatgtcattg acacatttaa agtgaataca caaaacgaag acgacatgaa gacattcgtt
      601 gagcaaacga tttatagcaa tgcatatcaa tcagatttaa aaatgtctat taccaaagcg
      661 ccccacttca aaaatcatag tcacgtgttt gatggtgata cacattgttg gctgattatt
      721 gaaacactgt acgcacaaac accttatcca attatgatta ataagtggta tattccacag
      781 gaaatatctg aattaacact gacacgtata agacaatcgg attattaaaa attattaaaa
      841 tataagaaat tttttgatga ctatttaggg agttggtaag aaatggaaaa gcttgttaca
      901 agcgcaattt cattcagtca actactgcca atataacatt agcgagccta ggacattgat
      961 ttatgtccta ggctcgctca gataatcaca cgatttttct attattaatc gtattatgtt
     1021 tatttatttg ttgaacgtcg tctaaatgct aagaataacc cagatactaa tagtagtgcg
     1081 gcaatacttg cagtaagaat acttgatgtc atactttcac cagcagctgg taaaactttt
     1141 ttgttacttt ggttcatttg atgatttgat gaatgcatat ttgcttttgg actcatcatt
     1201 tgatggtttg atgattgcat atgaccttgt tgcatgtgtg gctgttggtt catgtgtgtg
     1261 ttctgattca tatggccttg ttgactcata tgacctgttt tattttgcac catttcacta
     1321 ccaccatgat taacaccatt tgcatctaca ttcgtttgat aaaaattgcc atcttttgtt
     1381 aaaggtaacg catggccatt catatctcta acagcatgtg gtttaaatac ccctacatta
     1441 gttgtaatta tcacattgta tttatcagca atattttgcg tcgcatcata caatttcata
     1501 ttatagtctt gcatattact gccagcgctt aattggggta aagctttctc taattcagta
     1561 tattgtgctt gcgtcattcc ttgtggtact actccgccat aaggacgatg attttgtaca
     1621 ttttggcttt gtttattcga cacattcatt gaagttgtgt cggcatgcgc attttgaaaa
     1681 ggcatacttg agaatactaa agtgcttgtt acaattaaag aacctactgt tgctaatttt
     1741 ttcattgtct tgtcccctta tattacaatt tgatt
//
LOCUS       DAKTIP010000319         1763 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.324, whole genome shotgun sequence.
ACCESSION   DAKTIP010000319 DAKTIP010000000
VERSION     DAKTIP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.324"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(184..510)
                     /locus_tag="O0305_002780"
     CDS             complement(184..510)
                     /locus_tag="O0305_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCY0275868.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSANVEMFVEYV
                     KQHMIV"
     gene            complement(574..1317)
                     /locus_tag="O0305_002781"
     CDS             complement(574..1317)
                     /locus_tag="O0305_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0275869.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVNLNNK"
     gene            complement(1343..>1763)
                     /locus_tag="O0305_002782"
     CDS             complement(1343..>1763)
                     /locus_tag="O0305_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCY0275870.1"
                     /translation="ISESSKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKH
                     EVQCVICLPAALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDI
                     MQQHSYWKANFIERNIYDHSLMVSSNSIVHLKYHLEI"
BASE COUNT          608 a          300 c          188 g          667 t
ORIGIN      
        1 ttttaaaatt ttaatctata tcaacgtgtt tgatttgata ttttttatat taattggatg
       61 gcttgctcac aaaatttata aaatataaaa aaagaagagc tttaaatcat aacgcatgcc
      121 atttatcttt aaatgcaatg agttatcatt caaactcttc ttatcaacct acatacattg
      181 acattatact atcatatgtt gttttacata ttcaacaaac atttcaacgt tagcactttt
      241 aatatcatgc attgggacat taccaatatt aaatttgaac attatagttt gaccatcata
      301 atctatcttc tctatttcat tataactaat acttctataa taaaattggc cattcatatc
      361 tacattcata attaatcttt catttgttgc tataaatgcc ccttcaaatt cattctcacc
      421 ttgaacttga tactcaatta ttcctagcac tgacggaccc tttttttcag ttggaaataa
      481 atcatttgga ttcacattat ctaaaatcat aatatccctc ccacttaaaa ctataaacta
      541 ttcttcatag gtatatgaaa tacaagtgat taactattta ttattaagat taacttgtat
      601 tcccttttca agataattat aacatgcttg aataagcata gtgactttag aaaacttttc
      661 tcgcttgatt gttacaataa cacgcctttc atctaattca catctctctt ttttaactaa
      721 tcccttcaaa attaaacact ttaaaatcgg gctaaccgca aatatcgggt gatggaattc
      781 accctgtaat tctttaaccg tcaattgacc tttatgcaga tttaatattc ctaaaacgta
      841 taactcttct aatgataatt gacacatatt taaataacac tttagattcc gcaataattc
      901 ttgtgacgac aataaatacg atacatagtc acaactaaaa tcaaatgtat attgaccaaa
      961 ataacgttca atatagtcac taagctcatt attcatatat tctattttat ctatatgcat
     1021 tttattaatc gaaatagtca atttacgttg atccctttga tctcttcctt tcaatagcca
     1081 ttgatgattg tttaaataac ttaacatttg aagaagggcc atcttagatt ggatttcttt
     1141 catttttaat aaactatcta ttgaagcgct attggaatca gaattatcat atttaactaa
     1201 tagatttaat agtttcactt gagtcaaagt gagatgaaat gaatatttca acatatcatt
     1261 aatgactttc aacattagta tgtgtgcagt taaacgctta actttaaatt tagacatctt
     1321 taaaacctct cttaaaccat gcctatatct caagatgata tttcaaatga acaatactat
     1381 tgcttgagac cattaatgaa tgatcataaa tatttctttc tataaaatta gctttccaat
     1441 aactgtgttg ttgcataata tcattcacaa gtacaccatt ttcggaagta tgattatctt
     1501 tatctatact taaaacaatt tgtttagttt tagcatggct aaattgttga agacccttac
     1561 acgataaacg tatagcgtct gaattctcat ttaacaatgc ggctggcaaa caaatgacac
     1621 attgtacttc atgtttgaac ttcgttacaa tcatcgtgtc attttgataa ataacaatcc
     1681 ctcgtaattg attaagtata tttatgataa aaatgaatat tgataatcta gttaaattta
     1741 tttctttgct cgattcactt ata
//
LOCUS       DAKTIP010000320         1753 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.325, whole genome shotgun sequence.
ACCESSION   DAKTIP010000320 DAKTIP010000000
VERSION     DAKTIP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.325"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(99..896)
                     /locus_tag="O0305_002783"
     CDS             complement(99..896)
                     /locus_tag="O0305_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCY0275871.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
     gene            complement(1186..>1753)
                     /locus_tag="O0305_002784"
     CDS             complement(1186..>1753)
                     /locus_tag="O0305_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCY0275872.1"
                     /translation="IEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPH
                     VKEESVHLADMPKVVEVDQALLDKWRTFMNLRDDVNRALETARNEKVIGKSLEAKVTI
                     ASNDKFNASEFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERC
                     WNYSEDLGAVDELTHLCPRCQQVVKSLV"
BASE COUNT          575 a          332 c          231 g          615 t
ORIGIN      
        1 cattgcctaa gaacttaatg tcccagcctc cttcataact tgtataaaaa gatcattaaa
       61 aacgattcat tatatggttc gtccattcaa agtatatact aatcttcaac ttcgatatga
      121 ttaaagcgat aatagatacc atccggacct tgtaaattta aatgtgttgt ttcaggataa
      181 tgaaagtcaa caattgctaa tccatacgta tcaaaatttt ctacacgttt cgttgctgat
      241 acccaatcat tcatggccaa atgttgataa tagccattcg atgctaaaaa tattgaataa
      301 tcatccatat atgccgattg ctctaatcca aaataatcga ggtagtactc ttttacctct
      361 gataaatgaa ttgtcttaat atgcaatgca cctataatag cgtcatctgg aatacctaac
      421 cataacaatt ttgttgcatg actcactaat ctcggtacat ttaatggtct aatatcaatt
      481 tttactaaat tattttgcct cgtccattgc gcttcgtctt tatcaacata aaatttaaaa
      541 ccgtttcctt ccgggtcttc aaaaaataat gataacgaag caggctgtat acctccgtta
      601 actggaatat catattcgct taaatgaatt agtaaattag ctaaatcact aatttgaggt
      661 agtttaattg cgatatgaaa cagtcccgct tcggacatta aaggttcacg tccattttgt
      721 aattcaacaa gtgtaatgac atgattattt tgacctactt catattgtat cgatgttaat
      781 gtctcattta taatatttaa tcctaatatg tcctcataaa atggctttaa ttcattctta
      841 tctctcacat ttaaagtgat accattgaca aagtttgcgt tactgttatg atacattcaa
      901 ttaactccta tcaaattcaa tatctgtgct tacttttact taaaattatc gcgaacataa
      961 ttgagaatag caactataac gcatttatat gcctcagaga atttgtacca ttcctgaata
     1021 atttttattt tagtaaatcg cacatttttt acatatatga ccggtaactt ggtcattttt
     1081 ttattcacaa aaaaactctc ttcatcatgt caacaaaaac tacaacatga ttatgaagag
     1141 agctttaatt tatatcatct gtatgagtac tttatacaat ttcaattata caagtgattt
     1201 tacaacttgt tggcatcgtg gacatagatg cgtcaattca tcaacagcac caagatcctc
     1261 tgaatagttc caacatcttt cacatttttc accatctgca tgttcgatga caatatcacc
     1321 atgttcataa gctgttgcct gatcgtctaa cttatctaca actttaactt gtgacacgat
     1381 aaataattga tgtaatgcat caaatgaagt taagaattca gatgcattaa atttatcgtt
     1441 actagcaatc gtaactttag cttctaatga tttaccaata actttttcat tacgagcagt
     1501 ttctaatgca cggttcacat catcacgtaa attcataaat gtacgccatt tatccaataa
     1561 agcttgatct acttctacaa ctttaggcat gtctgctaag tgaacacttt cttctttaac
     1621 atgtggtgta tgagaccaaa cttcttcagc tgtatgcact aagattggtg ctaacaactt
     1681 cgtcatatca actaaaattt gatataacac tgtttgcata ctacgacgga tatgagaatc
     1741 acgttgttca ata
//
LOCUS       DAKTIP010000321         1749 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.326, whole genome shotgun sequence.
ACCESSION   DAKTIP010000321 DAKTIP010000000
VERSION     DAKTIP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.326"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..766
                     /gene="tagH"
                     /locus_tag="O0305_002785"
     CDS             <1..766
                     /gene="tagH"
                     /locus_tag="O0305_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCY0275873.1"
                     /translation="TKEYRIYRTNKERMKDALIPKHKNKTFFALDDISLKAYEGDVIG
                     LVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTGIENIEFKMLC
                     MGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITVNPDILVIDEA
                     LSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEGGKLKDYGELD
                     DVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            complement(827..1591)
                     /locus_tag="O0305_002786"
     CDS             complement(827..1591)
                     /locus_tag="O0305_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCY0275874.1"
                     /translation="MIVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
BASE COUNT          568 a          298 c          277 g          606 t
ORIGIN      
        1 aacaaaagaa tatcgtattt atcgtacaaa taaagaacgt atgaaagatg cgctcattcc
       61 caaacataaa aacaaaacat ttttcgcttt agatgacatt agtttaaaag catatgaagg
      121 tgacgtcata gggcttgttg gcatcaatgg ttccggcaaa tcaacgttga gcaatatcat
      181 tggcggttct ttgtcgccta ctgttggcaa agtggatcgt aatggtgaag tcagcgttat
      241 cgcaattagt gctggcttga gtggacaact tacagggatt gaaaatatcg aatttaaaat
      301 gttatgtatg ggctttaagc gaaaagaaat taaagcgatg acacctaaga ttattgaatt
      361 tagtgaactt ggtgagttta tttatcaacc agttaaaaag tattcaagtg gtatgcgtgc
      421 aaaacttggt ttttcaatta atatcacagt taatccagat atcttagtca ttgacgaagc
      481 tttatctgta ggtgaccaaa cttttgcaca aaaatgttta gataaaattt acgagtttaa
      541 agagcaaaac aaaaccatct ttttcgttag tcataactta ggacaagtga gacaattttg
      601 tactaagatt gcttggattg aaggcggaaa gttaaaagat tacggtgaac ttgatgatgt
      661 attacctaaa tatgaagctt tccttaacga ttttaaaaag aaatccaaag ccgaacaaaa
      721 agaatttaga aacaaactcg atgagtcccg cttcgttatt aaataaaccg aaaaaaccga
      781 gaatctccat ttaaggattt cctcggtttt atttttgtca tcatgattat ttcgcctttt
      841 ttatttttct ttttgctttg gctattttat acataaatat tggaatactc tttaatctac
      901 caatacgttt ccaatctatt aatgctctat atatccattc aatgtttaat tttctaaaga
      961 tataaggcgc tctcttttta gccccagcaa atacttcaag agaaccacct acgcccatca
     1021 tcactgtaga ttcaaattgg ttttcatgtg tcataatcca ttcttcttgt ttagggaatc
     1081 ccatacctac aaatatgtaa tcaggtttaa acagtttaat tcgtttcact actgtctcat
     1141 cttctaaatc aatataaccg tgatgatgtg caaacgatat gtttggatat ctttgttgca
     1201 atgcatattg tgccgcttct acaacttcat tagttgcccc tagcaaaaat actttttgat
     1261 gatttacatg agcaattttc aaacattcat ccatcaactc aataccaggt atacgatgcg
     1321 ctagaggttg ctttaaacga tgcgaagctt tgactactcc tgtcccatca gcaacaatat
     1381 agctcgcttg atttattaac tctaaatacg cttgatgtgt cgtcgcgtaa ttcactattt
     1441 cagggttggc tgttactata aaaagattat tcgttgattg atttgcaaaa aaggttttaa
     1501 tattttcaac catttgcaac attgttgtat tatcaaaatc gacccctaaa atgtcaactt
     1561 tggctgtatt ggatctttct tcaacaatca taacaggaat ctcctatctt atttcattat
     1621 tatgttagtg taatcaatat taacagtatt tatattcatc ctatttatat cgaaatgatt
     1681 ttgctttatt ttaacacata ttcacctttt caaaaattaa aattagttta actaaggtaa
     1741 gtctaaagt
//
LOCUS       DAKTIP010000322         1739 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.327, whole genome shotgun sequence.
ACCESSION   DAKTIP010000322 DAKTIP010000000
VERSION     DAKTIP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.327"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..514)
                     /locus_tag="O0305_002787"
     CDS             complement(<1..514)
                     /locus_tag="O0305_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0275875.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNNNEASAAAKPLDKSSSSLHH
                     GYSKVHVPYAITVNGTSQNILSSLTFNKNQNISYKDLEDRVKSVLKSDRGISDIDLRL
                     SKQAKYTVYFKNGTKKVIDLKAGIYTADLINTSEIKAININVDTKKQVEDKKKDKANY
                     QVPYTITVNGT"
     gene            900..1150
                     /locus_tag="O0305_002788"
                     /pseudo
     CDS             900..1150
                     /locus_tag="O0305_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187510.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            1472..1651
                     /locus_tag="O0305_002789"
     CDS             1472..1651
                     /locus_tag="O0305_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275876.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
BASE COUNT          617 a          230 c          218 g          674 t
ORIGIN      
        1 ttgtaccgtt cacagtgatt gtgtatggaa cttggtaatt tgctttatct ttctttttat
       61 cttcaacttg ctttttagta tctacgttaa tattaatagc tttaatttca cttgtattaa
      121 ttaaatcagc tgtgtaaata cctgctttca aatcgataac tttctttgtt ccatttttaa
      181 agtaaacagt atatttcgct tgcttcgata gtcttaaatc tatatcacta atacctctgt
      241 ctgattttaa aactgattta actctatcct ctaaatcttt ataactaata ttttgattct
      301 tattaaatgt taagcttgat aaaatatttt ggcttgtacc gttcacagtg attgcatatg
      361 gaacatggac tttagaatat ccatggtgta acgaacttga tgatttatct aatggcttag
      421 ctgcggcaga cgcttcatta ttattaaagt ttgcacctgt tgatgctaaa acacctaatg
      481 ctaaagttgt tgtaatcaat gacttaaatt tcataaatta tctctccttt tttgtgtaat
      541 tcgtatttgc aacttaatta tagccagact ttctctattt tttgaattaa ctgaatatta
      601 ataataaatt atctttaaca atattttttt aacactgtta aaagttcttt taattttgat
      661 taactaatta atttacaata cctaaaatgt tgtttggttt tgtttatacc aagcttcaaa
      721 cttaaatgtc ataacaacat tcatttctta attccgatta gatttgtcga ttatatttac
      781 agcatcttta tactcaaaaa acatttactt aaaaatataa attcgattta ataattaatt
      841 taaatttagt taatcaattt tgcatctatt ttgttgtaag ctatataaaa ggagtgataa
      901 tgatggtgaa aaaaacaaaa tccaattcac taaaaaaagt tgcaacactt gcattagcaa
      961 atttattatt agttggtgca cttactgaca atagtgccaa agccgaatct aagaaagatg
     1021 atactgattt gaagttagtt agtcataacg tttatatgtt atcgaccgtt ttgtatccaa
     1081 actggagact tttaacataa aattacttat cattcaaaaa gtaaaacagc ataatatcaa
     1141 ggtttataac tttatcatta tcaataatac ctcatataaa ataaaatttt agggactttt
     1201 tagggacttt aaatttaaaa ttacaagttt aatagaaaca tcaaaataat cacatgtttg
     1261 tgtggaatgt acaccccaaa agctagactg aaaaatctat tttttgaggt gtatttttat
     1321 aggtaaatat aataaattag agtagacaac tcagaattcc aattttataa taatattgct
     1381 tgacatatca aactagatag tactattttg aatatattat tataatcaaa aattcattgt
     1441 aaacttttag acaaaaggaa gtaataaaaa cgtgaaattt aaaacaacta aagaatgtaa
     1501 aagtaataat atctttaaaa gaagtcaaga aattaataat agagaaagtg aaaagggttg
     1561 tttatggggc attagcatgt tgattctact tttcttattg attctgtttg gaataactgc
     1621 ttgttcatca agcattcact ttattaatta gattttttta cttggaggta tcatgtgaag
     1681 aaccatacaa atataattaa tatcttatta gttatagtca actcattaac tcattttct
//
LOCUS       DAKTIP010000323         1728 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.328, whole genome shotgun sequence.
ACCESSION   DAKTIP010000323 DAKTIP010000000
VERSION     DAKTIP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.328"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            366..1133
                     /locus_tag="O0305_002790"
     CDS             366..1133
                     /locus_tag="O0305_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCY0275877.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHIFKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELDK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
     gene            complement(1181..1654)
                     /locus_tag="O0305_002791"
     CDS             complement(1181..1654)
                     /locus_tag="O0305_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCY0275878.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSICNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
BASE COUNT          606 a          289 c          241 g          592 t
ORIGIN      
        1 atgcatgagc aatgattgct gtaacaaatt ctagcaaagt attcagtttg tattttagaa
       61 ataaaaatga tgatagatgt atgatcgata taaaagattt aatatgcacc aaaatgataa
      121 cgtgagtaat tggtttaatg aaacataaca tgaatgtaaa tttagcttac tgacataaac
      181 actacacaaa catacagtta tataagtccc cttatggaat ttcacattct agtttacata
      241 atatatatta taggaagtta tatgtgtgta acgcaaaagg taccctacat cataatcatt
      301 atctaatatc gtcacataac ttacttatgc tataatcatg gtattatatt gtttggagtg
      361 atttgatgag atttgtcttt gatattgatg gtacgctttg ttttgacggc cgattaattg
      421 accagactat tattgataca ttgttacaat tacaacatga tggtcatgaa cttatatttg
      481 catcagcacg tccgattcgt gatttgttgc cagttttacc atcagtattt catcagcaca
      541 cattaattgg cgcaaatggt gctatgattt cacagcaatc aaagatttct gttatcaaac
      601 caattcatac tgatacatat catcatatct tcaaaataat tcaaaagtat gagttagatt
      661 atattattga tgatgattgg aattatgctg cacaacttga cgctgagaac gcgatttttg
      721 agcgtttaga tccacataag ctggccagtt gtattgatgt tgcaaatatc gacacaccaa
      781 tcaagattat tttattaaat atagacccgg cacaaattac aactatatta gacgagctag
      841 ataaatacca tcaagaattg gaaatgattc accattcaaa tgagtataac attgatataa
      901 cagcgcaaaa tattaacaaa tatactgcat tacaatatat atttgatgca gatgttaaat
      961 atatagcatt tggtaatgac cacaatgata ttgtcatgtt acaacatgct agtagtggct
     1021 atattatagg accatcagaa gcatacacac acgcaatatt gaaacttgat aaaatcaaac
     1081 acatcaataa taatgcacaa gctatttgca aagtcttaaa atcatataaa taaaaacacc
     1141 cctatcaaat gataatcatt atcaatcgat aggggctatt ttaataaaat tcgtcctcga
     1201 acatttcttc ctcttcatct aatccaaata attctgccat ttctccatgt tcaattaaca
     1261 tgtttaaata tgcatcgcgg agttcttctt cactcatatc attaatcatt tctttaagac
     1321 tatcaatcca catatttctg cgtaattgat agtcttcttc aacttcgttt aacatcatta
     1381 tatgtttatt tgctgcttct ggactagctg taaagagtaa tgcaatcata tgtttacata
     1441 tcactcgtct tccatcagca tgaggacaat tacatatgga ttttctagga tgttccatat
     1501 caatataaca acgatatact ttgttgccac tgccctttac ttcagcctca tgctgcgttt
     1561 ctgaaaatga ttttaagtta atgacgcatt cactttgata ataattaaag cctctttcta
     1621 tagaacgaat acttgcaata tcaagtaatc ccattaatga tactcctttt tattattatt
     1681 tttaaataaa gaaaataaaa tagataagtg tctagattaa aatacttg
//
LOCUS       DAKTIP010000324         1711 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.329, whole genome shotgun sequence.
ACCESSION   DAKTIP010000324 DAKTIP010000000
VERSION     DAKTIP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.329"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..512)
                     /locus_tag="O0305_002792"
     CDS             complement(<1..512)
                     /locus_tag="O0305_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HCY0275879.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKT"
     gene            complement(773..1582)
                     /gene="sgtB"
                     /locus_tag="O0305_002793"
     CDS             complement(773..1582)
                     /gene="sgtB"
                     /locus_tag="O0305_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCY0275880.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
BASE COUNT          509 a          287 c          226 g          689 t
ORIGIN      
        1 gttttagata ttttttcagc aatttggata atatcatcaa gttcctgttg ttctctataa
       61 agttctgtaa atatttcaat gatatttggt tctataccaa gttgatatag tttttgttga
      121 taaattttag gtcctttatc tgtcgtgcga atcattgtat tttttaaact ttccgcataa
      181 tcttgatggt cgattaactt ttcgcgataa caatattcaa tcacttcaga aatcgcttgc
      241 tctgatatct cttctttttg taaatattgt ataacttctt tttcagttct ctttttatat
      301 gataaatatt ggattgcttt atttaaacct atgcgataat gatcatactt ttgaatctct
      361 gccatgtcag cagcttcaag ttgttgccct ttttttaaat taaatttgac taatgtatca
      421 atatctatac ccatttcaaa ttgttcgtct aaaaaaagat taaaccgttc tttgtttttc
      481 ttttgaactt ctattttagt aatcttcggc actggatcac cccgttactt tcttaagttt
      541 taatacaata ctcttttatt attcaataag ccacttccta tagcaaatgt ttaactttaa
      601 atatttttcg atgctaacaa aaaatcacac tatcatcttt taaaatgaaa gtgtgattac
      661 aagcaaatct gtaaaattta taaagcagaa acaattcaac tttatcatta tgacatttca
      721 attaaacctt ctacattata gttccaagca tcttacacat gaatgcaagt atttaacgat
      781 ttaattgtga catagcctgt tgatattgtg tttcattgat ataattttgt tgcttcattt
      841 tttctaagtt cgtgcttaca cgttgcgtaa aattctctga catattattg atattatata
      901 cgctaggtgc attgacttta ctagctaaaa tagcgctttg taaaactgtt atgtgagaca
      961 ttgttgtact atttttattc acggttgttc caaagtaatg gtttgctgcg ccctcaagcg
     1021 tatattgatt atccccaaag taaatattat ttaaataaaa gcttaaaatt tcgttcttat
     1081 tatattgttt ttcaactcga tgagctacaa ataattcttt tacttttcta gtaaatgaac
     1141 gatcattatc ataaaaataa tttttgacaa cttgttgtgt aatggtacta ccaccttgca
     1201 catctctgtc gctaatcgtt gaaaataaag ctctagttgt acctttcaaa tcgaatccat
     1261 gatgattgta gaatcgttca tcttccattg aaataaaggc acctttaaca tactctggca
     1321 tgttatcagc tgacacaaaa ctacttttat tttcaatttt tcttagttca tccacattat
     1381 cgcgtgtaga taaaaaatac atgataccaa taaacaatgc gataatgatt agaatggtta
     1441 ataatatttt taatagtatt cgtttacttt ttttcttttt cggcggtttg ccaactggtt
     1501 gataatacgt attatagtga ggttcgtgtt tcatatgctc aaaatgttca tttgagtttg
     1561 agtacctatc gcttcttttc atgcgtttgc tccttctttt aaaactcact tagtatatac
     1621 cttgagttta ccagtactat cacaaatagg ctacactttt tgggaaaatc agtccaaggg
     1681 cttacaatcg tatacgccat catacttact t
//
LOCUS       DAKTIP010000325         1705 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.330, whole genome shotgun sequence.
ACCESSION   DAKTIP010000325 DAKTIP010000000
VERSION     DAKTIP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.330"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            30..995
                     /locus_tag="O0305_002794"
     CDS             30..995
                     /locus_tag="O0305_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCY0275881.1"
                     /translation="MSISEYKNRYLESLYNTFKTELENERTRYFILDTNYTNSDDTEK
                     EFTWDVKQNNKIREGDLFIFRRPTNLSQIAQQFYFFGAGKIEKIERKNNVATAYISKP
                     LLFVDRVLKDNIENLKWEFKERIKEEWEQFFFKNKISNITKNDFLKLLSLSRNVVEIE
                     SNYINNDISLTFKQMADMATRIKKNIYYVGNHEAFVQAKGSAHFEFSKRIKQNYSYRC
                     AITGIKTKDFLVVTHIIPWHENEFIRLDPSNGICLSLFLAKAFKKGFITFSNSYRVVL
                     SKEAEKDAALYEELKIYENQKIELPDCQKPNLKYLDWHREHIFKN"
     gene            1092..1283
                     /locus_tag="O0305_002795"
     CDS             1092..1283
                     /locus_tag="O0305_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275882.1"
                     /translation="MYYLAYGLTTLDNCFKYYQLITQNEHFCYSQQEERESYQQKFQS
                     MINGLKIKIGIQSSNDDAQ"
     gene            1375..1704
                     /locus_tag="O0305_002796"
     CDS             1375..1704
                     /locus_tag="O0305_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275883.1"
                     /translation="MARTYNVIKYVIKKNKDDLKIHGLRLYEFNIKDEKAINISQPIF
                     VNRATLIEKIQNGEAFTHAFRVNHNTYYAGQLISLSLNKNGYINNNFEASRDIIRNIE
                     VSILDYE"
BASE COUNT          688 a          185 c          281 g          551 t
ORIGIN      
        1 ttataattta caagtgagct gatagaaaga tgagtataag cgaatataaa aatagatact
       61 tagagtcact atataacacc tttaaaactg agttagaaaa cgaacgcact cgctatttta
      121 tattagatac caactataca aatagtgatg atactgaaaa ggagtttact tgggatgtaa
      181 aacagaataa taagatcaga gaaggcgatt tgttcatttt tagacggcca actaatttat
      241 ctcaaattgc tcaacaattt tatttctttg gagctgggaa aatagaaaaa attgagcgaa
      301 aaaataatgt agcaaccgct tacatttcaa aacccttact ttttgtggat cgtgttttaa
      361 aagataatat tgaaaattta aaatgggagt tcaaagaaag aattaaagaa gagtgggagc
      421 agtttttctt taaaaataaa atcagtaata ttacaaaaaa tgatttttta aaactactaa
      481 gtttgagtag aaatgtagtg gaaatagaaa gtaattatat taataatgat atttcgctaa
      541 cttttaaaca aatggctgat atggcaactc gaataaagaa aaatatttac tacgtaggga
      601 atcatgaagc atttgttcaa gctaagggaa gtgctcattt tgagttttca aaaaggatta
      661 aacaaaatta tagttataga tgtgcaatta ccgggattaa aactaaagac tttttagtcg
      721 ttacacatat tattccttgg catgaaaatg agtttattag actagatcct tcaaatggta
      781 tttgtttgtc tttattttta gcaaaagcat ttaagaaagg ctttatcact ttttctaaca
      841 gttatagagt tgttttatct aaagaagcag agaaagatgc tgctttatat gaagaattaa
      901 agatttatga aaatcaaaaa atcgaactac cagattgtca gaaacctaat ttgaaatatt
      961 tagattggca tagagaacat atttttaaaa attaattttg ggagaggttg tatgacagat
     1021 attagtcaat tagtagaggt gccatacatc tttcattagt agattgaaaa agagcacttg
     1081 aaacagataa tttgtattat ttagcctatg gattaacaac tttagataat tgttttaaat
     1141 attaccaatt aattacacaa aatgaacatt tttgctattc tcaacaagaa gaaagagaaa
     1201 gttatcagca aaaatttcaa agtatgatta atggtttaaa aattaaaatt ggcatacaaa
     1261 gttcaaatga tgatgcgcaa taagctacaa agtttgattt tttaattgaa cgttatatgt
     1321 ttttaaaaga agaaagagct tcgaattaat atcttaatgg aggaagatta gtaaatggct
     1381 agaacgtata atgtgattaa atatgtcatt aagaaaaata aggatgatct taaaatacat
     1441 ggattaagat tatatgaatt taatataaaa gatgaaaaag ctataaatat tagccaaccc
     1501 atttttgtga acagagcaac attgattgag aaaatacaaa atggtgaagc atttacgcac
     1561 gcctttagag taaatcacaa tacttattat gcaggccaat taatttcttt atcattgaat
     1621 aaaaatggtt atattaataa caactttgag gcctcacgag atattattag aaacattgaa
     1681 gtaagtattt tagactatga ataaa
//
LOCUS       DAKTIP010000326         1698 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.331, whole genome shotgun sequence.
ACCESSION   DAKTIP010000326 DAKTIP010000000
VERSION     DAKTIP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.331"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1568
                     /locus_tag="O0305_002797"
     CDS             <1..1568
                     /locus_tag="O0305_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275884.1"
                     /translation="ALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQKLMRLPLG
                     VVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLIDWRMASALL
                     VTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQSQSSYKKY
                     KDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLNYAEFFLCI
                     VLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQHYEIAFNNV
                     GFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVTSGEITIGG
                     INIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAKLAQCHEFI
                     EKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATVYVDPDNEQKIQE
                     ALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGNYKKMWDTH
                     MHTKDWGINTGHN"
BASE COUNT          613 a          226 c          306 g          553 t
ORIGIN      
        1 ttgctttaat cgcttatatt tcacaagtgt gttgttttgg agcgtcaacg atgctatcac
       61 atattacggc atatgaaatt ttatctgaaa ttcgtaaaaa attagctcaa aaattaatgc
      121 gtctcccttt aggcgtagtg gaatctaaga aaataggtga attaaaaaat atatttgtcg
      181 ataaggttga aacaatagaa ttacctttag cacatatgat tcctgaagtt ataggaaact
      241 tacttttgtc agctgctatc tttttataca taatgctcat tgattggcgt atggctagtg
      301 ccttattagt aacgatacca atttcaattt tcgcttttaa aaaagttatg tctggattta
      361 atgagacata tgctgaacaa atgaaatcga ataattatat gaatagcgcg attgtagagt
      421 ttatcgaagg cattgaagtg attaaaacat ttaatcaatc tcaaagttca tataaaaaat
      481 ataaagatgc agtagataat tataagattc acactttgaa ttggtttaaa aatacatggg
      541 ggtatatgaa tttaggagct agtgtattac cttcaacatt tttagggatt ttaccggtcg
      601 gcatgtattt gatatctatc aaccaattaa actatgcgga atttttccta tgcatcgtat
      661 tatctttagg tgtagtagca ccgattaaga attttactaa ttatgtaaat catttaaagt
      721 ctatacaata cgcattaact gaagtgaatc aaatattaag tctagaagag ttagtattgt
      781 caacaaaatt taagaagcct caacattatg aaattgcttt taataatgtt ggattttcat
      841 ataacaaaga taaggacgat ctggtcttta agcatttatc atttacagta ccagaaaata
      901 atttcacagc tatcgtcggg gcatctggta gcggtaagtc aaccattgcc aagcttatat
      961 cacgatactg ggatgtgact tcaggtgaaa ttacgattgg cggtataaat ataaaagata
     1021 ttgaatcgaa acaacttaac gatttagttg gttttgttgg acaagataac tttttattaa
     1081 atcttacttt taaagaaaat attaaacttg gcaatccaga agctacggat gaggcagttg
     1141 aaaaagctgc caagttagca caatgtcatg aatttattga aaagttgcca gatggatatg
     1201 atacgaatgt tggtacagtg ggagataaat tgtctggtgg tgaaaaacaa cgagtcacta
     1261 ttgcaagaat gatattaaaa gatgcgccta tcattgtatt agatgaagcg actgtttacg
     1321 ttgatccaga caacgaacaa aaaattcaag aagcattaaa tgtgttgacg caggataaaa
     1381 cattgattgt tattgcacat cggttatcta cgattcaaca tgcagatcaa attattgttt
     1441 taggtaaaca acaaatttta gaaaaagggt cacatcactt attgctaaaa ttaaacggta
     1501 attataagaa aatgtgggat acgcacatgc atacaaaaga ttggggaatc aatactggac
     1561 ataattaaac taatgagaaa aggaggtcaa atatgtttca aattacattt aaaattttaa
     1621 actggataag accatataaa gcaagaatga ttttaggatt tagcatgtca tttttaaatg
     1681 ctatttttat tgcgttac
//
LOCUS       DAKTIP010000327         1651 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.332, whole genome shotgun sequence.
ACCESSION   DAKTIP010000327 DAKTIP010000000
VERSION     DAKTIP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.332"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            317..823
                     /locus_tag="O0305_002798"
     CDS             317..823
                     /locus_tag="O0305_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275885.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
     gene            1082..1579
                     /gene="efb"
                     /locus_tag="O0305_002799"
     CDS             1082..1579
                     /gene="efb"
                     /locus_tag="O0305_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCY0275886.1"
                     /translation="MKNKLIAKSLLTLAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVK"
BASE COUNT          632 a          185 c          255 g          579 t
ORIGIN      
        1 aaacaaagaa atgattcata gtagagaagt aattaatatg tataatttta cttataaaga
       61 aataagagat atattctaat tatggttgat atgttatgat taatatataa gttaaaaatt
      121 atactattta attgaattat gcattttcga agtaaacata attcaacact tacattttta
      181 aatagataaa atagtgtaaa atcacgtgcc aagattacta tagagtaggc attgagtatt
      241 attggcaatc acttaagtga ttgttatttt tcgtggtcaa taataaagat ataaataaat
      301 atattgaggt caaacgatga taattaaaat tttaacaatt ctattactac tttgtatatt
      361 gagctatttg gttacaaata gaaagaagcc ttttctgttc ttaaagacac tctttatggg
      421 tgtggtattt atctttatag gatatatttc actggcaata tctgccgtaa ttatttatgg
      481 tattattcaa tttatcacaa ttgattttgg tagttttttc ttaatgggta ttatattgat
      541 cttgatttca agtatattcc aattatttat agttagatta ctttttagaa aaaagaatgt
      601 cgatttgaca gaggttgtcg ttttagagca tttaattcaa tggttcttag tttactttgc
      661 gatctatcaa gcagtaaatg aaaaaatgga cattaatgat attaatatcg acaatttcca
      721 atctgtcttt tttgacgtgt ctaatttgaa tttagtaatt ctaccaacgt taatcattag
      781 ctgggtcaca atatttaact atagaatgag aagttacaaa taaaatctat gagattatac
      841 cttcagacac caacattcaa atggtgtctt ttttgttgtg tggttttatt tttgaaattc
      901 gaaaaagtag aggcatgaat tttttgacta gtgtataagt gctgatgagt cacaagatag
      961 atagctatat tttgtctata ttataaagtg tttatagtta attaataatt agttaatttc
     1021 aaaagttgta taaataggat aacttaataa atgtaagata ataatttgga ggataattaa
     1081 catgaaaaat aaattgatag caaaatcttt attaacatta gcggcaatag gtattactac
     1141 aactacaatt gcgtcaacag cagatgcgag cgaaggatac ggtccaagag aaaagaaacc
     1201 agtgagtatt aatcacaata tcgtagagta caatgatggt acttttaaat atcaatctag
     1261 accaaaattt aactcaacac ctaaatatat taaattcaaa catgactata atattttaga
     1321 atttaacgat ggtacattcg aatatggtgc acgtccacaa tttaataaac cagcagcgaa
     1381 aactgatgca actattaaaa aagaacaaaa attgattcaa gctcaaaatc ttgtgagaga
     1441 atttgaaaaa acacatactg tcagtgcaca cagaaaagca caaaaggcag tcaacttagt
     1501 ttcgtttgaa tacaaagtga agaaaatggt cttacaagag cgaattgata atgtattaaa
     1561 acaaggatta gttaaataaa acttcaatcg ttgctgttat ctggaaataa ttaattaaat
     1621 gttatgttaa tttttgttaa tgaaaaaagt a
//
LOCUS       DAKTIP010000328         1645 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.334, whole genome shotgun sequence.
ACCESSION   DAKTIP010000328 DAKTIP010000000
VERSION     DAKTIP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.334"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(473..1171)
                     /locus_tag="O0305_002800"
     CDS             complement(473..1171)
                     /locus_tag="O0305_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
                     /protein_id="HCY0275887.1"
                     /translation="MIINELKSCKLKFSKQALTFVPIIVTILFILFINWYLNVNLWNG
                     RQISLFTASFNAITSLLISINVYQVINFEENIGHFNHILGKANRLNWLNASMIFTYTI
                     TAICILLASINLLWHSHDMKITLMFIGVSLFFNVIILLLLFIFSIFIKDVMAIVVGVL
                     MFIFNVYFGLEVLGDHSWFYLPITYATRYVYMFIEGSIPVTLTLAIYIIITLLLAVLL
                     FKGFNKWSGRTIKD"
     gene            complement(1168..>1645)
                     /locus_tag="O0305_002801"
     CDS             complement(1168..>1645)
                     /locus_tag="O0305_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HCY0275888.1"
                     /translation="TKEIRAKIFILSIAFLVHCFLVFIIAYIGDLIINPHPVNAMLLL
                     VTILLMYVVSLPLIPLNFLLTRYFGVFVSILINLVLSVICVLFLTLKSLFWVLPWGIM
                     QRIPLITLGILPNGLVVNHNSKYFNDLNALYISIIVSIIIFAIVTFLNNKKSWRLK"
BASE COUNT          639 a          283 c          191 g          532 t
ORIGIN      
        1 ccttaaactc aaatttcctt aattcataaa acattaataa ctaacccttt cttacaaacc
       61 acctagccca taatatatta aaacgtcatt tttatttcaa ttagcggaaa ataaaaaaac
      121 cattccgcat taactttggc aagttggaat ggttaaaaaa atattttaga agccagcgtt
      181 tggcttgctt agacgacatg ttcgcgcatg tcgtcttatt tttacattta tataataaca
      241 cgcaattcaa taaaatgtct aatcaataaa cctcaattac attcaaattc aaaaactttt
      301 cacctaatat tcatcgctta aataaacatt acgtttaatt aaacagacat tgtcaatcgc
      361 gtctaatacc cctgctgtcc gaaaacaaaa ttattttgta ttcatattta actgttctct
      421 ttcctccctt ttcaacgcaa ctaaatttct tttaccgtta gagccccaat ctttaatctt
      481 taattgttct accgctccat ttattaaatc ctttaaagag taaaactgct aatagcaacg
      541 tgataataat atagattgcc aatgttaatg taactggtat actcccttcg ataaacatat
      601 aaacgtaacg tgtagcatat gtgattggta aatagaacca cgaatgatct ccaagcactt
      661 ctaatccaaa ataaacgtta aaaataaaca ttaaaactcc gacaacaata gccattacat
      721 ctttaatgaa aatactaaaa ataaaaagta gcagtaatat aattacattg aaaaacaatg
      781 atacgcctat aaacataagt gttattttca tatcatgtga atgccacaat aaattaattg
      841 atgctaatag aatacatatg gctgtaatag tataagtaaa aatcattgat gcatttaacc
      901 aatttagcct attagctttt cctaaaatat gattaaagtg accaatattt tcttcaaaat
      961 tgataacttg atagacgttt atagaaatta atagcgatgt aattgcatta aaactcgctg
     1021 taaacaaact tatttgtcga ccattccata aatttacgtt taaataccaa tttataaata
     1081 atataaacaa tatggttaca ataatgggta caaatgtgag cgcttgctta gaaaatttca
     1141 acttacatga ctttaattcg ttaattatca ttttaatcgc caacttttct tattatttaa
     1201 aaatgttact atcgcgaaaa taatgatgct aacaataatc gaaatatata aggcattgag
     1261 atcattaaag tattttgaat tatggtttac aactaaacca ttaggtagta taccaagcgt
     1321 aataagcggg attctctgca ttatccccca cggcaacacc caaaataaac tcttcaatgt
     1381 taaaaacaag acacaaatga ctgataatac taagtttatt aatattgata cgaacacacc
     1441 aaagtatcga gttaataaaa agttgagcgg tatcaatggt agagatacta catacatcaa
     1501 caatattgtc accaataaca acatagcatt aaccggatgt ggattaataa ttaggtcacc
     1561 tatataagca ataataaata ctaaaaagca atgtaccaaa aatgctattg ataaaatgaa
     1621 aatctttgct cttatttctt ttgta
//
LOCUS       DAKTIP010000329         1627 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.335, whole genome shotgun sequence.
ACCESSION   DAKTIP010000329 DAKTIP010000000
VERSION     DAKTIP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.335"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            20..1381
                     /gene="dnaA"
                     /locus_tag="O0305_002802"
     CDS             20..1381
                     /gene="dnaA"
                     /locus_tag="O0305_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCY0275889.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
BASE COUNT          622 a          265 c          263 g          477 t
ORIGIN      
        1 agaaagatgg agtaaattta tgtcggaaaa agaaatttgg gaaaaagtgc ttgaaattgc
       61 tcaagaaaaa ttatcagctg taagttactc aactttccta aaagatactg agctttacac
      121 gattaaagat ggtgaagcta tcgtattatc gagtattcct tttaatgcaa attggttaaa
      181 tcaacaatat gctgaaatta tccaagcaat cttatttgat gttgtaggct atgaagttaa
      241 acctcacttt attactactg aagaattagc aaattatagt aataatgaaa ctgctactcc
      301 aaaagaaaca acaaaacctt ctactgaaac aactgaggat aatcatgtgc ttggtagaga
      361 gcaattcaat gcccataaca catttgacac ttttgtaatc ggacccggta accgctttcc
      421 acatgcagcg agtttagctg tggccgaagc accagccaaa gcgtacaatc cattatttat
      481 ctatggaggt gttggtttag gaaaaaccca tttaatgcat gccattggtc atcatgtttt
      541 agataataat ccagatgcca aagtgattta cacatcaagt gaaaaattca caaatgaatt
      601 tattaaatca attcgtgata acgaaggtga agctttcaga gaaagatatc gtaatatcga
      661 cgtcttatta atcgatgata ttcagttcat acaaaacaag gtacaaacac aagaagaatt
      721 tttctatact tttaatgaat tgcatcagaa taacaagcaa atagttattt cgagtgatcg
      781 accaccaaag gaaattgcac aattagaaga ccgattacgt tcacgctttg aatgggggct
      841 aattgttgat attacgccac cagattatga aactcgaatg gcaattttgc agaagaaaat
      901 tgaagaagaa aaattagata ttccaccaga agctttaaat tatatagcaa atcaaattca
      961 atctaatatt cgtgaattag aaggtgcatt aacacgttta cttgcatatt cacaattatt
     1021 aggaaaacca attacaactg aattaactgc tgaagcttta aaagatatca ttcaagcacc
     1081 aaaatctaaa aagattacca tccaagatat tcaaaaaatt gtaggccagt actataatgt
     1141 tagaattgaa gatttcagtg caaaaaaacg tacaaagtca attgcatatc cgcgtcaaat
     1201 agctatgtac ttgtctagag agcttacaga tttctcatta cctaaaattg gtgaagaatt
     1261 tggtgggcgt gatcatacga ccgtcattca tgctcatgaa aaaatatcta aagatttaaa
     1321 agaagatcct atttttaaac aagaagtaga gaatcttgaa aaagaaataa gaaatgtata
     1381 agtaggaaac tttgggaaat gtaatctgtt atataacagc actaatgata acaatcattt
     1441 tttacatttc tatatgctaa tgtggcaaga tgagcaaaac tcattttgtg gataatgttt
     1501 aaaagtcata cacaccatac acaagttatc aacatgtgta taacttcgcc aaatctatgt
     1561 ttttaagact tatccaccaa tccacagcac ctactactat tactaagaac ttaaaaccta
     1621 tataatt
//
LOCUS       DAKTIP010000330         1591 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.336, whole genome shotgun sequence.
ACCESSION   DAKTIP010000330 DAKTIP010000000
VERSION     DAKTIP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.336"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            3..335
                     /locus_tag="O0305_002803"
     CDS             3..335
                     /locus_tag="O0305_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0275890.1"
                     /translation="MRTNDEIITIIKTSMKEQNMSLSELARRVGVAKSAVSRYLNLTR
                     EFPLNRAEDFAKVLGIKTEYLLGFAEREESTKQDTIAAHLDGDFTEEELIEIRKYAEL
                     VRKAHRNQ"
     gene            347..805
                     /locus_tag="O0305_002804"
     CDS             347..805
                     /locus_tag="O0305_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCY0275891.1"
                     /translation="MYLYEKMVIENKEIPIDDGKSLGNFEGLYDNGVILINKNLSETR
                     KAEVLYEELAHHKLTYGNILDQSKWINRKFENYARRHGFISAVPLREIVEAYNYGVRN
                     LYELSEYLQLSEKYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI"
     gene            822..1550
                     /gene="seq"
                     /locus_tag="O0305_002805"
     CDS             822..1550
                     /gene="seq"
                     /locus_tag="O0305_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type Q"
                     /protein_id="HCY0275892.1"
                     /translation="MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPET
                     LQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYM
                     YGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNE
                     YNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKI
                     YNDNKTIDAENFHLDVEISYEKTE"
BASE COUNT          634 a          200 c          284 g          473 t
ORIGIN      
        1 aaatgagaac taatgatgaa ataatcacaa taattaaaac atcaatgaaa gaacaaaata
       61 tgtcactaag tgaattagct cgtcgtgtag gtgtagcaaa atcagcagta tcaagatatt
      121 taaatttaac tagagagttc ccattgaatc gtgctgaaga ttttgcgaaa gtacttggaa
      181 taaaaacaga atatttatta ggatttgctg aacgcgaaga atctacaaaa caagatacta
      241 tcgccgcgca cttagatgga gattttacag aggaagaatt aattgaaatt agaaagtatg
      301 cggagttagt tagaaaagca catcgaaatc agtaaggggt tattgcatgt atttatacga
      361 aaagatggtt attgaaaaca aagagatacc aattgatgat gggaaatctt taggtaattt
      421 tgagggtctc tatgacaatg gggttatttt aattaataaa aatttgtcag aaacacgtaa
      481 agcagaagtg ttatatgagg aacttgccca ccacaagttg acgtatggca acattttaga
      541 ccaatcaaaa tggattaata gaaaatttga aaattatgca cgtagacacg gctttatctc
      601 agctgttcca ttacgtgaaa ttgtagaagc ttataattat ggcgtacgta acttgtatga
      661 gttgtccgag tacctacaac tgagtgaaaa atacatatta gaagcgatag aacaatataa
      721 aaagatatac ggtattggta cccactacgg cgagtattca attacatttg agccgttgag
      781 agtttttaaa tataaagaaa tataacaaag gagaaatgaa aatgaataaa atatttagag
      841 tactcactgt tagcttgttt ttcttcacat ttttaataaa aaacaatcta gcatatgctg
      901 atgtaggggt aatcaacctt agaaactttt atactaatta tcaaccagaa acgcttcaag
      961 gagttagttc tggaaatttt tctacttctc atcaattaga gtatattgat ggaaaataca
     1021 ctttatattc acagtttcat aatgaatatg aagcgaagag attaaaagat cataaagtag
     1081 atatctttgg aataagttac tcaggtcttt gtaatacaaa atatatgtat ggcggaatta
     1141 cgttggcgaa tcaaaattta gataaaccta gaaatatacc tattaatctc tgggtcaatg
     1201 gtaagcaaaa cactatatct acagacgagg tttctactca aaaaaaagag gtaactgctc
     1261 aagagattga tattaagtta cgaaaatacc tacaaaacga atacaatata tatggtttta
     1321 ataaaacaaa aaaaggtcag gaatatggat atcagtcaaa atttaattct ggatttaaca
     1381 aaggaaaaat tactttccat ttaaataatg aaacttcttt tacatacgat ttgttttaca
     1441 ccggaactgg tcaagcagag agttttttaa aaatttacaa tgataataaa actatagatg
     1501 cagagaattt tcatttggat gtagagattt catatgagaa gactgaataa atagaattta
     1561 aggagaaaat gcaatgaaaa aattaatagt t
//
LOCUS       DAKTIP010000331         1578 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.337, whole genome shotgun sequence.
ACCESSION   DAKTIP010000331 DAKTIP010000000
VERSION     DAKTIP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.337"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..85
                     /locus_tag="O0305_002806"
     CDS             <1..85
                     /locus_tag="O0305_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275893.1"
                     /translation="SVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            638..1504
                     /locus_tag="O0305_002807"
     CDS             638..1504
                     /locus_tag="O0305_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCY0275894.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKVI
                     SSVYVHPEPDHL"
BASE COUNT          530 a          226 c          294 g          528 t
ORIGIN      
        1 tagtgttaac tatgtaaaaa agcgtttaat tcagaacatt cagcgtattt taagtaagga
       61 agtactatat ggagaaatgc aatagtttgc taataaatag aggagaaaat tgtctttcga
      121 agagagtttg gtagagtaga aatctcattt aatttgtcat tgatgcactt tttcttctat
      181 ataattagtt cacgacatta gatagcttcg caactcatgc atagggttct aacatagaaa
      241 ctttcgaggc gaaagttaac aaacagggag agttgggtgc ctttctaaca aataaatttg
      301 aagaagacgg aggaacgcag ttggacgtaa aacgtcaact gcataagcct ttctagctca
      361 taaaattcgc agaaaataaa ggaacacatt agatagcttc gctactaatg catagggtcc
      421 caacatagaa actttcgagg cgaaagttaa caaacaggga gagttgggcg cctttctagc
      481 tcataaaatt cgcagaaagg ctaaaaatta tttttagggg ttgcgaatga ataaattagt
      541 ttaattttat aaaaatttat aaaaattaaa aacgttgtaa aattaaaatg aatcattcat
      601 aatattgtta ctatactaat tcaaggtggt tcaacgcatg tctcataatg aaaatcttaa
      661 attggcgcaa cgcggtgctt atttgagttt aattgtatat ataatcttat ctattgttaa
      721 gtatgtcacg ggttttgtct ttaactctgc agctgtaaga gccgatgcat taaacaatat
      781 gacagatatt atcgtttctt tagctgttat tattggattg aaaatttcaa ttaaacctgc
      841 cgatcgaaat catccttatg gccatttgaa gtctgaaaat atttcttcat tattggtgtc
      901 atttgtcatt atgtttgtag gtatccaagt agttattcaa aatgcacctc gtttgttcaa
      961 agaagatgac gttgtaccta acgcaataac aattatcgtc agcttaatca gtggtcttgt
     1021 aatgttgatt gtatttgcag tcaatcaaag actagcgaaa agaacgaaaa gtagttcttt
     1081 aaattcggct gccaaagata atttatcgga tagtttagtc agcattggaa cagcgatagg
     1141 attaattttt acgcaaatcg gtttcccaat tgtcgatatt attttagcaa cattactagg
     1201 cttactaatc gtttatactg gttttggtat ttttaaagaa gcaattttta tgttgagtga
     1261 tggtttcaat gaaactgaat tagaagcgta tcgcaatgat attttagaag ttgatgaagt
     1321 acaagaagtt aaaagtatta aagggcgtta ccatggaagt agtgtgttta ttgatgtcac
     1381 aattgttgta gatgctaacc tttccttagt agaagcgcat caaatttgtg acaatgtcga
     1441 acatcatttg cacaaaaaag ttatttcctc agtatacgtt cacccagaac cagaccatct
     1501 ataatgtatt taaacgtctt catagatata taaatatctg aggaggcgtt tttattacat
     1561 ctatttttag gtttacct
//
LOCUS       DAKTIP010000332         1573 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.338, whole genome shotgun sequence.
ACCESSION   DAKTIP010000332 DAKTIP010000000
VERSION     DAKTIP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.338"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            3..>1573
                     /locus_tag="O0305_002808"
     CDS             3..>1573
                     /locus_tag="O0305_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCY0275895.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNQSDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGN"
BASE COUNT          699 a          166 c          265 g          443 t
ORIGIN      
        1 gaatgaaacc attacattta aagttgaata atttcggccc ctttttaaaa gaagaaattg
       61 atttttctaa aattgataat aatgaattgt ttttaataag tggtaagact ggatcgggta
      121 aaacaatgat ttttgatgca atgacttatg ccttgtttgg taaagcatca actgaacaaa
      181 gagaagaaaa tgatttgaga agtcatttcg ctgatggtaa acagccgatg tcagtaacat
      241 ttgaatttca attaaatcat cgaatttata aagtgcatag acaaggccct tatatcaaag
      301 aaggtaatac aacaaaaacg aacgctaaat ttgatgtatt tgagatggtg gatggcaagt
      361 atgaaattag agaaagtaaa gtaatttcag gtacccaatt cattattgaa ttattaggag
      421 taaatgcaga tcaattccga caattgttta ttttgcctca aggtgaattc aaacgctttt
      481 taatatcaaa cagtcgtgaa aagcaaggga tattaagaac actgtttgac agtgaaaaat
      541 ttgaagctat acgagaaata ttaaaagaag aagtaaaaaa agaaaaagct caaatcgaga
      601 atagatatca acaaattgac cttttatggc aagaaattga atcatttgat gatgacaata
      661 taaaaggctt attagaggtt gccactcaac agatagacaa attgattgaa aatataccac
      721 ttttacaagc taggtcgaaa gaaatactag catctgtaaa tgaaagtaaa gaaactgcta
      781 ttaaagaatt tgaaataata gaaaagaaaa cattagaaaa taatatatta aaagataata
      841 ttaatcaact caacaaaaat aaaattgatt tcgttcaatt gaaagaacaa caacctgaaa
      901 tagagggaat tgaagctaag ttaaagttgt tacaagatat tacaaaccta ttgaattata
      961 ttgaaaatag agaaaaaatt gaaactaaaa ttgctaatag caaaaaagat atttctaaaa
     1021 ccaataataa aatattgaat cttgattgtg ataagcgaaa catagacaaa gagaaaaaaa
     1081 tgttagaaga aaatggagat ttaattgaaa gtaaaatctc ttttattgat aaaactagag
     1141 tattatttaa cgatattaat aagtatcaac aaagttatct caatattgaa cgcttgagaa
     1201 ctgagggtga acaattaggt gatgaattaa atgatctaat taaaggttta gaaacggtcg
     1261 aagattcaat aggtaataac caaagtgatt acgagaaaat tatcgaacta aataatacga
     1321 taacgaacat aaataatgaa attaatataa ttaaagaaaa tgaaaaagct aaagctgaat
     1381 tagataaact attaggtagt aagcaagagt tagagaatca aattaatgaa gaaacatcta
     1441 tattgaagaa tctcgaaata aaattagatc gctacgataa aacaaaattg gacttaaatg
     1501 ataaagaaag ctttataagt gaaattaaat ctgctgtaaa tattggagat caatgtccga
     1561 tatgtggtaa tga
//
LOCUS       DAKTIP010000333         1551 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.339, whole genome shotgun sequence.
ACCESSION   DAKTIP010000333 DAKTIP010000000
VERSION     DAKTIP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.339"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..882)
                     /gene="truB"
                     /locus_tag="O0305_002809"
     CDS             complement(<1..882)
                     /gene="truB"
                     /locus_tag="O0305_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCY0275896.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTK"
     gene            complement(1051..1401)
                     /gene="rbfA"
                     /locus_tag="O0305_002810"
     CDS             complement(1051..1401)
                     /gene="rbfA"
                     /locus_tag="O0305_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCY0275897.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
BASE COUNT          472 a          294 c          180 g          605 t
ORIGIN      
        1 ttttgtaggg tgtaccatat aaattgctaa tactttttct gaatcatcat caataaatac
       61 aatttggtct ttaattttgt tatcaaattc atttttatta aatttctgcc catttaaaat
      121 acgttttttt atgtgcgaat ctttaatttt aatgcttggc aaacccttta atccatattc
      181 taaaggaaac aatttatttt gcaatgaatc ctgctcatga agttctttta tttgttctaa
      241 tgtaaggcta tctttcaaca caaatccacc agactcgatt cgtgttaatt tcgacatatg
      301 tgccggaaag cctaatttca caccaatatc agttgctagc gttctaatat atgtaccttt
      361 accacagatg acgcgtattt taaaatgaca ctcattttct ttaaaatcta attcagatat
      421 acgcccaatg tctttaatat taactttacg ctttggtctt tcaactgttt cattattacg
      481 cgcatattca tataattttt taccatttac tttgacggat gagtacatcg gcggaatttg
      541 ttcaatgata cctttaaaac tttctaacaa tcggtcaata tcgtccttat taaaatctgc
      601 tgagtgtaca ccttttgttt ccaatgtatc acccgtttga tcttcagtcg ttgtacttct
      661 tcctatcgat acagttgctt cataagcttt gcccatatcc ataacataat cactaactct
      721 cgttgcatta cctatacata ccggtaacac gcctgcaact tcgggatcaa gcgtacccgt
      781 gtgacctatt tttttagttt ttaatatttt acgcaatttg aatacaacgt catgacttgt
      841 taaaccgcgc tctttatata ctggtaatat cccattatac atatagtcac cttctttttt
      901 aaaatatatt ttaatagcgt atcaactaaa tttattcata aaaaaactgg aaaatgttaa
      961 ctatttctac acatctatgt attaactgtt taatgttaat ataccgatat taagaaatta
     1021 tcaaacattt tccagatacc taacactaaa ttatctatct tgtttgtgta aatcttgaat
     1081 cattcgttca attttattac catattcgat tgattgatca tattcataca ttaattccgg
     1141 cataatacgt aatcgcattc tagaacctaa ttcagactta atgaagcctt ttgctttatc
     1201 aagtgcttta aatgtatttt ctacttcttt atcgttacct aatacagtta aaaatacttt
     1261 agcctgcgat aaatcatttg ttaaaacaac atctgtaatt gtaataaaac caactcgagg
     1321 atctttgact ttattgttga tgatatccat taattccttc ttcatttgtt caccaacacg
     1381 ctctgctctc atactgctca ttcttgtcac ctctttatct ttaagttcaa ttgttatttg
     1441 cattttttag tgtaacaatg cttaaacaat tattttagct cacacgttat tttaataaat
     1501 taagcctata aaatcaaata aagtttgccg taagataggc atttcaacag a
//
LOCUS       DAKTIP010000334         1531 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.340, whole genome shotgun sequence.
ACCESSION   DAKTIP010000334 DAKTIP010000000
VERSION     DAKTIP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.340"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            156..1310
                     /locus_tag="O0305_002811"
     CDS             156..1310
                     /locus_tag="O0305_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCY0275898.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMNNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPLFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          542 a          235 c          230 g          524 t
ORIGIN      
        1 cattttgaaa agtagttact acagtgtatt tagacatttt tcgtttttca aataaagctt
       61 aagtaattta cccttttttg ctttaaaagt atgttatgat attattaatt tctaaatttt
      121 tagacaatat ttgatttata tttgaggtgt cacaaatgaa actttcttta aattctaatt
      181 ctaaatattt aagagcacca agtattcgcc aattttcaaa tcgtatgaat aatttggatg
      241 attgtgttaa tttaactatt ggccaaccag acttcccgat gcctgatgtt gtgaaaaagg
      301 catatattga tgctattaac aatgacaaga catcatactc tcacaataaa gggttattag
      361 aaactcgcga agcaattagt caatacttca aaaatcgtta tcatttttcc tatgatcctg
      421 aagaaattat tgtgacaaat ggagcaagtg aagcaataga cacaacgtta cgttctatca
      481 tagagcctgg agatgaaatt ataataccgg gaccaattta tgcaggctac ataccactca
      541 tcgaagtact aggtggtaaa ccaatttata ttgatacaac agcaacacaa tttaaaatta
      601 cacctgatgc attagaaagt catatttctc caaagacaaa agctgtcttg ttaaattatc
      661 cgactaatcc aactggcgta gttttaaaaa gaaatgaagt tttaaatatc gtaaatgtat
      721 taaaaaaata tccgttattt attattagcg atgagattta tgctgaaaat acatttagtg
      781 gtaaacatgt atccttcgct gaatttgaag acattcgtga tcaattaata ttaattggtg
      841 gtttaagtaa atcacactca gcaaccggaa taagaattgg ttttctatta ggacctcaat
      901 atttgattga taaattaaca tttatgcacg cctataattg tatttgtgcc aatgttccag
      961 ctcaaatagc atgtataaca gcacttaacg aaggattaga agctcctaaa tatatgaacg
     1021 aagcttatgt agaacgaaga aattatttag tatctgaatt aactaaatta ggttttgaga
     1081 ttaccgctca acctgaaggc gcgttttata ttttcccaag tattaaacat atcaccgatg
     1141 acgatttcga attttgtgtc gatttacttg aatcaacaca tttagcaatc gttccaggct
     1201 catcctttac agaattcggt aaaggatttg taaggatttc ctatgcttat gaaatggatg
     1261 ttttaaaaga aggtatgaag agacttgcta aatatttaaa tactaaataa aaatgatatg
     1321 ttgaactgga cgtcgtgaac taaattcata gtacgttcag ttttttatat tctataagtt
     1381 ttaaataata ctctatagag actgtaaata ctaccgagtt atattcctct cctttaaaca
     1441 cctcactcaa aatgattcta tcaaaaggtt ttaatatgct aacttataat tgttcttcga
     1501 aacttaagca ctcaaataat atatcaccat t
//
LOCUS       DAKTIP010000335         1517 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.341, whole genome shotgun sequence.
ACCESSION   DAKTIP010000335 DAKTIP010000000
VERSION     DAKTIP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.341"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            403..>1517
                     /gene="fmtA"
                     /locus_tag="O0305_002812"
     CDS             403..>1517
                     /gene="fmtA"
                     /locus_tag="O0305_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCY0275899.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNN"
BASE COUNT          619 a          178 c          229 g          491 t
ORIGIN      
        1 aaaatataat tagttgaacg atatttatac aacttcatta ctaatacatt atagccttaa
       61 attgtagtac tttatagtaa aaaatacaaa tgttatgtca aagttccttg taatttgaat
      121 gttttgttaa aatcaaaaag catttttaaa aatataatgc gactttagta tgatgtctta
      181 aaaaatttaa aggcgtaata taaattcgag tgatttatac aaagtgataa ggtataatta
      241 attttgttgt atagataaca tattagaaaa acttattact atgtagcgta aagcataaat
      301 gaatgataag ttaatttttg taagttgtat tattaggcat ctaggttcaa atttttaacg
      361 atttaaatat ataaaatgaa tacattgtga ggcaattatc aaatgaaatt taataaagta
      421 aaactagtta tacatgcgtg tgtactatta tttatcatta tttctatagc gttaattttt
      481 catcgattac agacgaagac acattctata gacccaatac ataaggaaac aaaattatca
      541 gacaatgaaa aatatttagt ggatcgtaat aaggaaaagg ttgcgccgtc taaactaaaa
      601 gaggtatata atagcaagga tcctaaatat aagaaaattg acaagtattt acaaagttca
      661 ttatttaacg gttcagtagc tatatatgaa aatggcaaat tgaaaatgag taaaggttat
      721 ggatatcaag attttgaaaa aggtattaaa aacacaccga atacgatgtt tttaataggt
      781 tcagctcaaa aattttcaac agggttactg ttaaaacagt tagaagaaga acataaaata
      841 aatatcaatg atccagtaag taaatacctt ccatggttta aaacatctaa gcctatccca
      901 ttgaaagatt taatgttgca tcaaagtgga ttatataaat ataaatcctc aaaagattat
      961 aaaaatttag atcaagcagt taaagcgatt caaaaacgtg gtattgatcc taagaaatac
     1021 aaaaagcata tgtataacga tgggaattat ttagtacttg cgaaagtaat tgaagaagtt
     1081 acaggtaaat cttatgctga gaattattat acaaaaatag gagatccttt aaaacttcag
     1141 cacacagcat tttatgatga acaacctttt aaaaaatatc tagcaaaagg ttatgcttat
     1201 aatagtacag gactttcatt cttaagacct aatattttgg accaatacta tggtgcaggt
     1261 aatttatata tgacaccaac agatatgggt aaattaatta ctcaaataca acaatataaa
     1321 ttattcagtc ctaaaataac caatccatta ttacatgagt ttggtacgaa aaagtatcca
     1381 gatgaatatc gatatggttt ctatgctaag ccaacattga atagacttaa cgggggattc
     1441 tttggacaag tctttactgt ttactataat gataagtatg tagttgtact tgcattaaat
     1501 gtaaaaggaa acaatga
//
LOCUS       DAKTIP010000336         1498 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.343, whole genome shotgun sequence.
ACCESSION   DAKTIP010000336 DAKTIP010000000
VERSION     DAKTIP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.343"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(179..1483)
                     /gene="eno"
                     /locus_tag="O0305_002813"
     CDS             complement(179..1483)
                     /gene="eno"
                     /locus_tag="O0305_002813"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCY0275900.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
BASE COUNT          444 a          298 c          237 g          519 t
ORIGIN      
        1 ttattagtct gttatgatta atacttcaaa ataaaaaacc taccacagtc tcaactctgt
       61 ggcaggctat aaatttaagc gtgaacttat ttaagcgtga actcaaaatc tttatttaat
      121 ttataccgtc ataataatcc tcaactaaaa ttatgtcagc atttgattat aaagaaaatt
      181 atttatctaa gttatagaat gatttgatac cgtcatattt agcagtttca aataattcat
      241 cttcgatacg taataattga ttgtatttag caatacggtc agtacgtgat aatgaaccag
      301 ttttaatttg accagcgttt gtagcaacag caatatcagc aattgttgta tcttctgttt
      361 cacctgaacg gtgagaaact actgctgtgt aaccagcttt ttgagccatt tcgattgcat
      421 caaatgtttc agttaatgta ccgatttggt taactttaat taagattgag ttaccaattc
      481 cgttttcaat accttttgct aaaatttcag tgtttgttac gaataaatcg tcacctacta
      541 attgtacacg gtcaccgata cgttctgtaa gttgtttcca accatcccag tcgttttcgt
      601 ccataccgtc ttcaattgta atgataggat atttgtctac taattgttct aagtagtcaa
      661 cttgttctgc agctgtacgt tttgcaccgt gttcgccttc gaacttactg tagtcatata
      721 caccattttc atagaattct gatgatgcac agtcaaatcc taagaatact tcttcacctg
      781 gtttgtaacc agctgcttcg attgcttgga taattgtttc aacagcatct tcagtacctt
      841 caaatttagg agcgaaacca ccttcgtcac ctactgcagt ttctaaacca cgtttgctta
      901 aaattgattt taagttgtgg aaaatttcag taccccaacg taatgattct ttgaacgttg
      961 tagcacctac aggtaaaatc atgaattctt ggaatgcaat tggagcatct gagtgagaac
     1021 caccattaac gatgttcatc attggtactg gtaactgctt accattaaat ccacctaaat
     1081 atttgtaaag tggttgacct aataagtcag ctgctgcacg tgctactgcg atagatacac
     1141 ctaaaatagc atttgcacct aatttacctt tgtttggagt accgtctaat gcgatcatca
     1201 ttttatcaat agatacttga tctaatactg aaaattcacc ttcaataatt tctggtgcga
     1261 tgatttcatt aacgttttca actgctttag taacaccttt acctaaataa cgtgatttgt
     1321 ctccatcacg taattcaaca gcttcgtgtt caccagttga agcacctgat ggtactaatg
     1381 cacgaccaaa tgcgccactt tcagttaata cttctacttc aacagttggg ttaccacgag
     1441 agtctaagac ttcgcgagcg taaacatctg taataattgg catgtttata atctcctt
//
LOCUS       DAKTIP010000337         1496 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.344, whole genome shotgun sequence.
ACCESSION   DAKTIP010000337 DAKTIP010000000
VERSION     DAKTIP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.344"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..586
                     /locus_tag="O0305_002814"
     CDS             <1..586
                     /locus_tag="O0305_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0275901.1"
                     /translation="NQVIIGGDSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKG
                     AILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTF
                     LSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKV
                     LLFLSRNTSSSGIQTEEKPQIENPSNELPLNPLN"
     gene            915..1343
                     /locus_tag="O0305_002815"
     CDS             915..1343
                     /locus_tag="O0305_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275902.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
BASE COUNT          590 a          180 c          247 g          479 t
ORIGIN      
        1 taatcaagta attattggcg gtgattctgc aggtgctcaa ttagctagcc aatttacggc
       61 aatacagacg aatgatcgct taagagaagc catgaaattt gatcagtcat tcaaaccatc
      121 gcaaattaaa ggtgctatac tatttggtgg tttttataat atgcaaacag ttagagaaac
      181 tgagtttcca agaatacagt tatttatgaa aagttatact ggcgaagaag attgggaaaa
      241 gagttttaaa aacatttcac aaatgtcgac agtaaaacaa tcgacaaaaa attatccacc
      301 aacattttta tctgttggag atagcgatcc attcgaaagt caaaatatag aattcagtaa
      361 gaaattacaa gaattgaatg taccagtaga tactttgttt tatgatggta cgcatcattt
      421 acatcatcag tatcaatttc accttaataa acctgaatcg atagataata tcaaaaaagt
      481 gttacttttc ttaagtcgta atacatcctc tagtggtatt caaactgaag agaaaccaca
      541 aatagaaaat ccgagtaatg aattaccgtt aaatccttta aactaatgat aaacagtagt
      601 aatttattgc ttaagcaaca tttaagattt tcaaattaaa aacgaagaat ttaaaacatg
      661 tggtgctaat gtgtaagagt ctgagatata ataaattgtt agcggttctt tatcatttct
      721 atctcactct attatgatgt gacatttatt ttacaaacta atttgttttg aacctgaaaa
      781 taacttttta caactaaatt gtcgaaaaac agtgtgtaca tgatataata aatttataaa
      841 ttgaaaagaa ttcaaaagaa aaattaaaat atagattgag cacgaagtga tttgaaataa
      901 ggttgtgaaa gggaatgaca aggtcagcat taaaaccatt taaaaataaa cgcgttatgg
      961 ttactggacg tatacaacgt gttttgttta aaaattattt agatagacat agcacattta
     1021 agccgaatgt aaggatatta ttaaaagatg tatttgtttc aggtgtatca atagatcatt
     1081 tatggttata tgagacaaat aaatactatg cattggcaat ggaacttatt catcaacgag
     1141 taaaatttag tgcgaatgtt gtaccgtatt acaaaataaa tagaaataat aatttattcg
     1201 tacaagatta tggaattaag cgtaaaggta ggttaattac tgaagaagct tacaatcaaa
     1261 acaatcagta tcaggataag atatatgaaa aattaccgga tatagatttt agactcgaag
     1321 atttttatag taaggaaaac taaacaacat atataaagca ccactaatcg tagttgatag
     1381 ttaatcaatt acacattagt ggtgttttat tttgaattta aatttgataa atgaataaga
     1441 gtgtacgtat ttatttgaga ttagataaca ttgataagta tgagtaatgt attagg
//
LOCUS       DAKTIP010000338         1476 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.345, whole genome shotgun sequence.
ACCESSION   DAKTIP010000338 DAKTIP010000000
VERSION     DAKTIP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.345"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            85..621
                     /gene="amaP"
                     /locus_tag="O0305_002816"
     CDS             85..621
                     /gene="amaP"
                     /locus_tag="O0305_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCY0275903.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            634..873
                     /locus_tag="O0305_002817"
     CDS             634..873
                     /locus_tag="O0305_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCY0275904.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            936..1445
                     /locus_tag="O0305_002818"
     CDS             936..1445
                     /locus_tag="O0305_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCY0275905.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT          569 a          199 c          263 g          445 t
ORIGIN      
        1 atttattatg tttaagaaaa aaactgatgg gtacatattt aatatgtttt taaattaact
       61 ttaactaagg aggtgtcgtt aggggtgaaa cgacttaaaa actttatcct tggcttatta
      121 atagtagtaa tcgtggggtt cctgttattc atgtatatcc aagatggacg tataactgaa
      181 tatcaagact acttccttca atttgaatgg ttccaaccat tacttatttc acttgctgca
      241 ctattgatat taataggtct tatattagta tttagtatct tcaaacctac gcatcgaaaa
      301 cctggattat acaaagactt cgatgatggt catgtttacg tatctcgtaa agctgttgaa
      361 aagacggcat tcgatacggt tgcaaaatat gatcaagtaa gacaaccaaa tgtagttgcg
      421 aaactatata acaaaaaaaa taaatcttat atcgacatta aaactgactt tttcgtacca
      481 aataatgttc aagttcaatc tttaactgaa gcaattcgtt cggatattaa aaagaatgta
      541 gaatatttca cagaaatgcc agtacgtaag ttagaagtta atgtgagaga tcagaaaact
      601 tctggtccac gagtgttgta agggaggtat atcatggcta acaatcataa ccaaaacgga
      661 caagactcta cacaacaagt gattaatttt ttaaaagtat ttaaatggag aatcgttggt
      721 ttcctagcgt ttctattaat cgcgatatta ttcttaacgt taggtttttg gaaaacagta
      781 cttatcatcg ttttatgttt aattggtgta ggtattgggt atatgaaaga ccgtaagcaa
      841 gattttatga attttttaaa tcgatggagt taattcatca atttagaatt taattaaaaa
      901 aatataaaaa ttaacaagta aaaggagaat atattatgac tgtagataac aataaagcaa
      961 aacaagcata cgacaatcaa actggtgtta acgaaaaaga aagagaagag cgtcaaaaac
     1021 aacaagaaca aaatcaggag cctcaattca aaaacaaatt aacattctct gatgaagttg
     1081 ttgaaaaaat tgctggtatc gctgcacgtg aagttaaagg tatcttagat atgaaaggtg
     1141 gcttaactga tacattcact aatgcattct caagtggcaa caatgttact caaggtgtat
     1201 ctgttgaagt tggtgaaaaa caagctgctg tagacttaaa agtaatctta gaatatggtg
     1261 aatcagcacc aaaaatcttc cgtaaagtaa ctgaattagt taaagaacaa gttaaatata
     1321 ttactggttt agatgtagtt gaagttaaca tgcaagttga cgatgtaatg actcaaaaag
     1381 agtggaaaca aaaacacgaa aaaaataacg aaaacaataa ccaagaaaga caaggtttac
     1441 aataagttat tcgataaaaa gggcttggga cattaa
//
LOCUS       DAKTIP010000339         1454 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.346, whole genome shotgun sequence.
ACCESSION   DAKTIP010000339 DAKTIP010000000
VERSION     DAKTIP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.346"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            231..416
                     /locus_tag="O0305_002819"
     CDS             231..416
                     /locus_tag="O0305_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275906.1"
                     /translation="MNKHINKKAAKLLEDYKKVQQRKSKETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
     gene            complement(1069..1419)
                     /gene="scb"
                     /locus_tag="O0305_002820"
     CDS             complement(1069..1419)
                     /gene="scb"
                     /locus_tag="O0305_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-B"
                     /protein_id="HCY0275907.1"
                     /translation="MKFKKYILTGTLALLLSSTGIATIEGNKADASSLDKYLTESQFH
                     DKRIAEELRTLLNKSNVYALAAGSLNPYYKRTIMMNEYRAKAALKKNDFVSMADAKVA
                     LEKIYKEIDEIINR"
BASE COUNT          474 a          224 c          198 g          558 t
ORIGIN      
        1 ctattcattt atgtattcta gtgcttcatt aatattttaa tgcatcagtt taagtaaatt
       61 ccatttggaa aataaaaagc aaatatcatc atcaaattaa catatttaac gttatacttt
      121 cataaaattt gaaacaattg aacatcctta ctttagcttt atatctttat ttcaagtcat
      181 gttttcaact attttaatga attaaacata tattataagg agtcaacatc atgaataaac
      241 atataaataa aaaagccgca aaactacttg aagattataa aaaggttcaa caaagaaaat
      301 cgaaagaaac tgatagaggt tgtttgaaaa gtgtcatcat gcttatcgtc ttatttttct
      361 taattatttt tggtattgtg gcatgttcta caaacgtaaa ctttttcttc cagtaaaaga
      421 gtgagataga aatgataaag aaccactaat gatttattat gtagtggttc tttatcatta
      481 gctgtagcta atgtgtactt aaaaaatgaa tacatgagta aagctcatgc ataagaaata
      541 ctaattgcta aagaaaaagt atttctttat cgatgtccta ccccaacttg cattgtctgt
      601 agaaattggg gttccaattt ctctatgttg gggccccgcc aacttgcaca tttttgtaag
      661 ctgacttttc gccagcttct atattggggc ccccgccaac ttgcattgtt tgttggaatt
      721 ggtgatccta tttctctctg ttgtggcccc acgagtattg aaaaaacttg ttataagctt
      781 attttcattt agtcaactac taccaatata gcactagaac tggaaatgat aatttaatat
      841 tgtacacttt tgattgttta aacatgtaga tattttaaaa aataggagag caaagtaaat
      901 aattgatata gttattttga gagtaatcct aggaactatt gtatttatat ttgtctcccc
      961 tacttttaaa tgtcattcat tatacataag cattttgata tagaatttat cacatatgca
     1021 aattgaaaac aggttaagac cattttttgt ctcaacctgt tttatttatt atctatttat
     1081 aatttcatca atttctttgt atattttttc taatgcaact ttagcatcag ccattgatac
     1141 gaaatcattt ttcttaagtg ccgctttagc tctatattca ttcatcataa tcgtacgttt
     1201 ataatatgga tttaagcttc ctgcagctaa tgcatataca ttcgatttgt taagtaaagt
     1261 tcttaattct tctgctatgc gtttatcatg aaactgactt tcagttaaat atttgtccag
     1321 actacttgca tctgctttat tcccttctat agttgctatc ccagttgatg ataaaagtaa
     1381 tgctaatgtt cctgttaata tatatttttt aaatttcatt ataagctctc cttaaaattg
     1441 atacttactg agta
//
LOCUS       DAKTIP010000340         1434 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.347, whole genome shotgun sequence.
ACCESSION   DAKTIP010000340 DAKTIP010000000
VERSION     DAKTIP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.347"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            83..811
                     /locus_tag="O0305_002821"
     CDS             83..811
                     /locus_tag="O0305_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCY0275908.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKQKDDDHSGLDEV"
     gene            896..1149
                     /locus_tag="O0305_002822"
                     /pseudo
     CDS             896..1149
                     /locus_tag="O0305_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1165..>1434
                     /locus_tag="O0305_002823"
     CDS             1165..>1434
                     /locus_tag="O0305_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275909.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILE"
BASE COUNT          650 a          154 c          241 g          389 t
ORIGIN      
        1 tcaactttgt ttaattactc aaaatgataa gtcatttaga gtttttaaaa taaatttttg
       61 cgaaataaag gagacatgtt aaatgaaaaa agtaatgggg atattattag caagtacact
      121 tatcttaggt gcttgtggac atcatcaaga tagtgcaaaa aaagagagca ctagtcacaa
      181 aaagaaagaa aatgacaatg aagaattaaa tgaagaactt aaagaattta aaagcaaaaa
      241 aaatatggat ataaaaatta aaggcgatac tattgttagt gacaaatttg aagctaaaat
      301 aaaagaaccg tttatcatca atgaaaaaga tgagaaaaag aaatatatcg cttttaaaat
      361 ggaaattact gctaaaaaag acgataaaga tttaaatcca tcttctattt ctcatgacta
      421 tattaatatc actcaagatg ataaaaatac agtaaataaa ttaagagatg gttatctttt
      481 aagtgataaa aaatataaag attggacaga acataaccaa gatcaaatta aaaaaggcaa
      541 aactgcacaa gccatgttta tctatgagtt aagaggtgat ggaaatatta atttaaatgt
      601 ccataaatac tcagaagata aaacagttga ttctaaatca ttcaaattta gtaaacttaa
      661 aaccgaagat ttttctcata gagcggaaac aagagaagaa gtagaaaaga aagaaaaaga
      721 atttgaagaa gagtacaaaa aagaacaaga acgagagaaa gaaaaagaaa agcaaaaaga
      781 tgacgaccac agtggtttag atgaagtata aatttggttt gttgattgtc ttacattcag
      841 ttatttaatt tttagagaaa ctgacttatt tatagcttag ccaacatgat actataaagc
      901 tatgattaaa aataaaaaat ctgtgtttaa tggtgttaaa gaaatcagtc cccgtgccgg
      961 aaaatcataa aaaaaagatg ggataaattt gtaaatcccg cattgaataa tgcattaaaa
     1021 caagaagagg ctacatgggg aaatgtacaa ggtcaagttt cacaagcatt gatgggaact
     1081 ggtattaaag attctactgc aagaagcata gggttttggg tttcccaagt tggtcaagca
     1141 ttaatataaa aagagtgtga taaaatgaat aatgaagaat tagaaatgag attgttatta
     1201 atgaaacaat caatagaaca attacaagaa gaactagcgc ctaacttaaa aactagagat
     1261 ttagtgttat taagatatat gtattcatat aaagaaatta atatgctaga ttcttacttg
     1321 ttccaattgg ctacaaataa agagcaaata acaaaaaaac aatttaaaac aaaattggaa
     1381 aacattagag aagtaccaga aatacctatt agacaagtta atgatatatt agaa
//
LOCUS       DAKTIP010000341         1433 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.348, whole genome shotgun sequence.
ACCESSION   DAKTIP010000341 DAKTIP010000000
VERSION     DAKTIP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.348"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..960
                     /gene="ribF"
                     /locus_tag="O0305_002824"
     CDS             <1..960
                     /gene="ribF"
                     /locus_tag="O0305_002824"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCY0275910.1"
                     /translation="EVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEIAEAR
                     SLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRFANVS
                     VEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIENEKIS
                     TTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYLLPRK
                     GVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERVTVNW
                     HHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            1075..1344
                     /gene="rpsO"
                     /locus_tag="O0305_002825"
     CDS             1075..1344
                     /gene="rpsO"
                     /locus_tag="O0305_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCY0275911.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
BASE COUNT          509 a          212 c          264 g          448 t
ORIGIN      
        1 gaagtgacac atcctataca atctaaacag tatattacag aggatgttgc aatggcattc
       61 ggatttttcg atggcatgca taaaggtcat gacaaagtct ttgatatatt aaacgaaata
      121 gctgaggcac gcagtttaaa aaaagcggtg atgacatttg atccgcatcc gtctgtcgtg
      181 ttgaatccta aaagaaaacg aacaacgtat ttaacgccac tttcagataa aatcgaaaaa
      241 attagccaac atgatattga ttattgtata gtggttaatt tttcatctag gtttgctaat
      301 gtgagcgtag aagattttgt tgaaaattat ataattaaaa ataatgtaaa agaagtcatt
      361 gctggttttg attttacttt tggtaaattt ggaaaaggta atatgactgt acttcaagaa
      421 tatgatgcgt ttaatacgac aattgtgagt aaacaagaaa ttgaaaatga aaaaatttct
      481 acaacttcta ttcgtcaaga tttaatcaat ggtgagttgc aaaaagcgaa tgatgcttta
      541 ggctatatat attctattaa aggcactgta gtgcaaggtg aaaaaagggg aagaactatt
      601 ggcttcccaa cagctaacat tcaacctagt gatgattatt tgttacctcg taaaggtgtt
      661 tatgctgtta gtattgaaat cggcactgaa aataaattat atcgaggggt agctaacata
      721 ggtgtaaagc caacatttca tgatcctaac aaagcagaag ttgtcatcga agtgaatatc
      781 tttgactttg aggataatat ttatggtgaa cgagtgaccg tgaattggca tcatttctta
      841 cgtcctgaga ttaaatttga tggtatcgac ccattagtta aacaaatgaa cgatgataaa
      901 tcgcgtgcta aatatttatt agcagttgat tttggtgatg aagtagctta taatatctag
      961 agttgcgtat agttatataa acaatctata ccacaccttt ttcttagtag gtcgaatctc
     1021 caacgcctaa ctcggattaa ggagtattca aacattttaa ggaggaaatt gattatggca
     1081 atttcacaag aacgtaaaaa cgaaatcatt aaagaatacc gtgtacacga aactgatact
     1141 ggttcaccag aagtacaaat cgctgtactt actgcagaaa tcaacgcagt aaacgaacac
     1201 ttacgtacac acaaaaaaga ccaccattca cgtcgtggat tattaaaaat ggtaggtcgt
     1261 cgtagacatt tattaaacta cttacgtagt aaagatattc aacgttaccg tgaattaatt
     1321 aaatcacttg gtatccgtcg ttaatcttaa tataacgtct ttgaggttgg ggcatattta
     1381 tgttccaacc ttaatttata ttaaaaaagc tttttacaaa tattaacatt tat
//
LOCUS       DAKTIP010000342         1428 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.349, whole genome shotgun sequence.
ACCESSION   DAKTIP010000342 DAKTIP010000000
VERSION     DAKTIP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.349"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1198)
                     /locus_tag="O0305_002826"
     CDS             complement(<1..1198)
                     /locus_tag="O0305_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275912.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYK"
BASE COUNT          558 a          220 c          184 g          466 t
ORIGIN      
        1 acttatattt ccgaaattct tttaataaaa taattaaaat gattacatgt atcgtataaa
       61 caatcaaaaa attacctggt tctattggag taacaatgac tagtgtcgac gccgaaacac
      121 atattgcaat aagtaaggaa tataaagtga tttgtgtttt acgatcatta atagacaaat
      181 aaatacctac aaatatcgaa aacgcaaagt aaccacatac aattacgttc acaaaaccaa
      241 ccaatccaat atctgtattt ttatttaata aaaactgatt gtaaaatagt aaataataaa
      301 gcggtaaagt gataaatcct atcataatga tacgacgctt taatattgtt aaatgtacat
      361 accgatcact tctaagcagt agataaataa taagcgctgc aatcatactt aaaattataa
      421 tctgactgaa aatgacacca tatggaagtg acgttgatat catttctgca aattttgaaa
      481 agataccttg attatttgaa acttgttgat attctgatcc ttcaaataaa attttgcgat
      541 aatttggatt ggaaaacata atgattgtac ctatagtagc aatcataaat gaaaacaata
      601 atttataatt aagtgttcga tttgcagtaa attcatacag aaaagctaaa attaaaatga
      661 tacagtggaa taatgtcaca ttctccataa ataattgacc gaagaagcat aaaacataga
      721 atagtacagt aacacttacc ggctgctttt ctttatatat aattgcatta atacaataat
      781 aaataataaa gagtgaaatt agtgttgatg tcgcataatt ataaaatcct gcaaaccagc
      841 catatgtatc tgcataaata gcacttggta aaattaacat taaagaaaat gctaacaaat
      901 aatagcttgt ccacgcttta caacgtgtaa tgtgcataat catccaaatg atgcccatac
      961 taatgaggcc atatgaaagc caacgtaacc agcttacatg tacagctata atttcaaaga
     1021 tatttccgat atagcgacca ttaagtgatg caaatcctac ttttaaaata tcagtattat
     1081 aattgctaaa ccattgtaaa tcatcgtgca tgagtggtag taagataccc ataaaagtat
     1141 aaaacaataa tatcgcaatt aatatcaaag ttgtcttgtg taattgaatt gttttcactt
     1201 tgctaatcct caaatctagt taaattttcc tcaacttgta ggtcgaaaaa ttaattcaat
     1261 atttttaatg tatttctaat tttcacctat gcatgtttcc tcaatcaaat tagataaaca
     1321 aggtatttaa tattactttc aacaatttat ctaaatcgcc cctcgtcttt ttctatgacg
     1381 aatgattaca cttgtctatt caagtgttta aattaagatt tatttccg
//
LOCUS       DAKTIP010000343         1428 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.350, whole genome shotgun sequence.
ACCESSION   DAKTIP010000343 DAKTIP010000000
VERSION     DAKTIP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.350"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            98..418
                     /locus_tag="O0305_002827"
     CDS             98..418
                     /locus_tag="O0305_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCY0275913.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            415..1056
                     /locus_tag="O0305_002828"
     CDS             415..1056
                     /locus_tag="O0305_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0275914.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQIKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFGLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(1144..1410)
                     /locus_tag="O0305_002829"
     CDS             complement(1144..1410)
                     /locus_tag="O0305_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275915.1"
                     /translation="MKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTASALWFFPG
                     AIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT          576 a          180 c          234 g          438 t
ORIGIN      
        1 tatttgcaac gattcttgtc gttcaaatat tattgcagat ttgcagccta tactatcatg
       61 gccgacgttt taatgaagtt atcaaggagt tttaactatg aaatttatca ttgcaatatt
      121 attaggatta atattatcca ttactattgc ttttacaatc atacatcatc ctatacttga
      181 tcgttttaat cctttcttaa aaacggagta tagttatgcc aaagtgccaa aaggtacgca
      241 acaatatgtt aatattacgg cttatagtga aagaggggaa aagcttgatt ataaattaac
      301 atttaatgga ttttcaccta gtagaacgta tgtcgaaata aagcataaag ggcaatatgt
      361 catatcgatc acatatgttg aaaaagagga tacaccaaaa gaggtaagac aagaatgata
      421 gaattaaaag atttaaccat acaaaaaggt aatacacata ttttgaagaa acttaatttg
      481 aaatttcaat gtggaaaatc atatgcactt attggtaaaa gtggatgcgg taaatctaca
      541 ttattaaata ctattgctgg acttgaaaaa acaggaaaac aatatgtcta ttttaatggt
      601 caattagaac aatttaaatc taatttttac agagataaat taggatattt atttcaaaat
      661 tatggattaa tcgataattt gacagtaaat gaaaatttag atattggatt agcatataaa
      721 aaaataagta agaaagaaaa agaacaaata aagatacgtt atatagaaca gtttggtctg
      781 tcaaacagtt taaaaagaaa agttcatacg ctaagtggag gtgaacaaca acgtgtcgct
      841 ttaattagaa tgatgttaaa agatccgatt gttatgttag ctgatgaacc aacgggtgcg
      901 ttagatccta aaacaggaca gatgattatt caatcattat ttggtttggt cgatgaaaat
      961 aaagtgctga ttttagcaac acatgatatg gctattgcaa atcaatgtga cgaaataata
     1021 gatttagaac agtatagaaa agtagcatct atgtgatatc caaaagataa aattaaaagc
     1081 caatcaatgg gaaatgttga cttcaacact ccgtttgatt ggcttttata ctgtgttact
     1141 aagttatgaa agtaacaacg gcaaactgaa aaaaagcaat ataataatat ttaatacgat
     1201 tagtaataaa tgtaattgat ttttcgtttt aatatagcaa aaaacaatga ctaaagataa
     1261 caacaatatc atgattgcac ctgggaagaa ccataatgct gaagctgtgt accaaatcat
     1321 tggtaacgcc ataataataa aagatataag acaagaaagg ataccaataa tattataagt
     1381 tctagtcaaa attacacctc caattttcac tttaaaatga catgcgta
//
LOCUS       DAKTIP010000344         1426 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.351, whole genome shotgun sequence.
ACCESSION   DAKTIP010000344 DAKTIP010000000
VERSION     DAKTIP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.351"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..370
                     /gene="sigS"
                     /locus_tag="O0305_002830"
     CDS             <1..370
                     /gene="sigS"
                     /locus_tag="O0305_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCY0275916.1"
                     /translation="LIKMWQLSQIYKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDV
                     ILCENNSPTLTEQPTYFNEHDLRLQDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLS
                     LSTIKLIKMSVKRKCQHNFN"
     gene            483..926
                     /locus_tag="O0305_002831"
     CDS             483..926
                     /locus_tag="O0305_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCY0275917.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(913..1356)
                     /locus_tag="O0305_002832"
     CDS             complement(913..1356)
                     /locus_tag="O0305_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275918.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQNVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMFASIYFIKIVTSYFTKKLLEIKFNSK"
BASE COUNT          530 a          237 c          167 g          492 t
ORIGIN      
        1 cttgataaaa atgtggcaat tgagtcagat atataaaccc tcaagcaagc aatctttatc
       61 ctctttttta ttcactcgat taaattttta ccttatcgat ttattcagac aacaaaatca
      121 attaaaagat gtcattttat gtgagaataa ttcaccaaca ttaactgaac aaccaacata
      181 ctttaatgaa catgaccttc gtttacaaga tatcttcaag cttttaaatc aaagagaaag
      241 actatggctc aaactatacc ttgaaggata caagcaattt gaaattgctg aaatcatgtc
      301 attatcgctt tcaacgatta aattaattaa gatgtccgtt aagcgtaaat gccaacataa
      361 ttttaattag tttattgtga aatttcggtc gatgtgttaa tgatgttgaa acaccctaac
      421 atcttcatat aaatttattt taaccattaa attaacaact tattaaacga ggagtactat
      481 atttgcaaga caattctact aaatatctac tttatatcca aactgctact tcaaaccatc
      541 tcgaaacaaa ttgtgtcttt ttacattgcg attacattct taaagttcca attaacaaac
      601 tcgtttcata ttatgcgaaa ttgcatttat catcacaaag tgtgctaatt gagactgcaa
      661 aaaacatact aaatattaat aaactggttc ctatttacat caacccaaaa actatacttt
      721 ttccgttaaa acataaacgt gcaccgatac aaatttatat caatgcacat tatattgttg
      781 gtatgactgc catagagaat tcaacattaa tacattttca agaaggcatt cagctagaag
      841 ttgatgagcc attttctcta gtttcaaaaa aatgtcatga aagtttagct ttgaagcact
      901 tcattgaaaa tactatttcg aattaaattt aatttctaaa agtttcttag taaagtatga
      961 tgtcacaatt tttataaaat atatgctagc aaacattaat aaaatcgctc caataattgt
     1021 taaaataaca acagttgtag ataaatctac cttaggacta acgaaaaatt gtacgccaga
     1081 tagacttgta attagcccaa ataaaaacaa acctaataaa gcaacataag caaacatcat
     1141 cactactaat gtaacaaaca tactataact ttttgtttct ttatttcgaa atacattttg
     1201 tcttaagtaa gcattagcaa aggtaacagg cttatttaag ttaacgctgt catcatcaga
     1261 tatagaagac aatcgttgat ctaatgcttg tttctccttc ttgttcaaaa accataattg
     1321 ctttttaact tttttatcaa atgatgcttt attcataatt aaggatttct ctcctttata
     1381 atattgacgt aattttaaac attagcgtca attgaaagtg tatttt
//
LOCUS       DAKTIP010000345         1422 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.352, whole genome shotgun sequence.
ACCESSION   DAKTIP010000345 DAKTIP010000000
VERSION     DAKTIP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.352"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(3..1346)
                     /locus_tag="O0305_002833"
     CDS             complement(3..1346)
                     /locus_tag="O0305_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0275919.1"
                     /translation="MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRV
                     IQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQL
                     LLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVS
                     SIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT          549 a          243 c          184 g          446 t
ORIGIN      
        1 ttctattctt ttgattgaga tgactgttct ttaaaaaata cgacccataa aacaataagc
       61 ccaatgctaa ttacagcaac agcacttaac acattgtgta agtttggacc aaagattcct
      121 gattgtatcg cataaagata tcccattatt gttgaaccta ttgatgcccc aagattttta
      181 gttaatccat aaaatgacat cattttcttc atttgtaatg ggctagtttc ctcttggaca
      241 atcacactat ctttcgtata aatatatcca aaacttaatc ctgccaaaat taacacaaat
      301 gctataagta ccggcaattt aataccaaat gaaatgataa tactacttac tagtagcaat
      361 gtaaatgata gtaagtaaat aactttcctt gatagttttg cttcaattcg atgtaaatta
      421 aaattcaatg taatccaagc tacagataaa ggaaaaataa ccaatccact ttgcaatgga
      481 gataatccta gttgttcttg aaggtagact ggaatatata aattgaatcc cattaaacaa
      541 atagctgtta gaaggtcagt tataaaaact aaagtaatcg aacgattaaa ttcaaccaca
      601 ggtaaaaatg gtgaagaaac atgtttttct actttaaata aacacattgc cacaacgata
      661 gctaagataa aactcaagaa atttaaaagt aacagctgtt ggtttaataa agcaaacata
      721 atcaatccaa taaatacata aaatagcgtg agccctttag tatcaaattt tgatttagca
      781 actgtttctt cctctggaaa atgaaatgtc catataacta atataatggc aatgatcgca
      841 atcggtatat ttatgtagaa taaccaatgc catgttgcaa attctaatat accaccacct
      901 agaagtggcc caatgatact cgaaataccc caaacactgc caactatgcc cataatttta
      961 tatcgtaacg gaatttcaaa tgctaacttt ggaacaattt gcgataatga catcaataca
     1021 cctgatccaa aaccttgtat cacgcgtgat ataattagca tagtaaaatt agtacttaaa
     1081 ccacacataa aactacctat actaaataaa agtaatcctg caattgccac ataaataatt
     1141 ttaaatctcg aaagtaattc gcccactata ggattggcaa ttactaatgc gataaaatac
     1201 gctgtgaaaa ttaatgaaat taaatttcct gcatttaaat cttgtttaat tgtaggtaat
     1261 gcaagcgaaa taatagatga ttcaatcgct gacataaaca taattaatat taaagctata
     1321 acagttatga ttgactttaa tcgcatgaaa tacctccttc tatatattta tttataagat
     1381 tttatttaca aataattggt tttcatatgt ataaacactt tt
//
LOCUS       DAKTIP010000346         1398 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.353, whole genome shotgun sequence.
ACCESSION   DAKTIP010000346 DAKTIP010000000
VERSION     DAKTIP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.353"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            302..703
                     /locus_tag="O0305_002834"
     CDS             302..703
                     /locus_tag="O0305_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCY0275920.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSITWY"
     gene            663..755
                     /locus_tag="O0305_002835"
     CDS             663..755
                     /locus_tag="O0305_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275921.1"
                     /translation="MLKTVKSTRLLGIKKHTELNSCTPNVRKQC"
     gene            complement(1072..>1398)
                     /gene="ecb"
                     /locus_tag="O0305_002836"
     CDS             complement(1072..>1398)
                     /gene="ecb"
                     /locus_tag="O0305_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCY0275922.1"
                     /translation="KKNFIGKSILSIAAISLTVSTFAGESHAQTKNVEAAKKYDQYQT
                     NFKKQVNKKVVDAQKAVNFFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLVL
                     KYNTLK"
BASE COUNT          497 a          209 c          192 g          500 t
ORIGIN      
        1 tatccaactt agcaaacaaa atgtagagaa atgtaaatat cgttttagca ataaaaatta
       61 ttcaatcata gttgagtgac gtttactact attattatac tatcaacaaa ttcaataata
      121 cctcaaataa aaataatacc atcaaattca tcattattct ttttcgattg cattaaacct
      181 tattaatttg cacttaattt taattaatga agtgttaact ggtaaatgta tgaaaccgtt
      241 tgtaaaatag cagtgtactc gcattagtaa gtacaaattt attttacagg gagaacttat
      301 tatgaaaaaa aatatcacaa aaactattat cgcatctaca gtaatcgctg caggtctttt
      361 aactcaaact aatgatgcta aagctttctt tagttatgaa tggaaaggtt tagaaatcgc
      421 aaaaaattta gcagatcaag ctaaaaagga cgatgaacgt attgataagt taatgaaaga
      481 gtctgataaa aatctaactc cttacaaagc tgaaactgtt aatgatctgt accttattgt
      541 taaaaaatta agccaaggtg atgtgaagaa agcagttgtc agaattaaag atggtggtcc
      601 tagagattac tatacttttg acttaactcg tcctttagaa gagaacagaa aaaatattaa
      661 agttgttaaa aacggtgaaa tcgactcgat tacttggtat taaaaaacat actgaattaa
      721 atagttgtac gccaaacgtt agaaaacaat gctaacgtat tggcgtgctt ttttatttta
      781 agtaacttcc aatttattta gcatctttac gactgtttaa taaagcacgt atgattaaca
      841 cggttgccac tatatccgtt acaattttta tgattgttaa tacattcgat ttccttttca
      901 caatagccac ctcgcttgtt caaaacataa acaactattg cattcacttt taagtaatta
      961 tttatattat ttatcccaat aagctcacca ttcaaataaa cacaatatta taaaataatt
     1021 atacctttgg atatagcaaa aagccacact ctatagcatg gcttctatca attattttaa
     1081 agtattatat tttaaaacta gatcgatttg tctttgtaat tttttctttt cataactgtg
     1141 ttggaaatga attaaattaa cagctctttg tgctttacgg tgtgttgcaa cagttcttgt
     1201 acgtttgaaa aagtttacag ctttttgtgc atccacaact tttttattta cttgtttttt
     1261 aaagtttgtt tgatactgat catatttttt agcagcttca acgtttttag tttgtgcatg
     1321 agattcaccg gcaaacgttg ataccgttaa actaatagca gctatgctta aaattgattt
     1381 cccaataaaa ttcttttt
//
LOCUS       DAKTIP010000347         1373 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.354, whole genome shotgun sequence.
ACCESSION   DAKTIP010000347 DAKTIP010000000
VERSION     DAKTIP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.354"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..195
                     /locus_tag="O0305_002837"
     CDS             <1..195
                     /locus_tag="O0305_002837"
                     /inference="COORDINATES: protein motif:HMM:NF037202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0275923.1"
                     /translation="GKPAIEWIIDQYNVSIDKKSGILDDPNEFSEDPNYILNLLLSVI
                     TVSMKTLGLIDKLPDFEVLD"
     gene            256..999
                     /locus_tag="O0305_002838"
     CDS             256..999
                     /locus_tag="O0305_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275924.1"
                     /translation="MDLLQIEKKGNLTKTVQLMHDEKGSASGESKVNMEFGKQSAVKQ
                     IFDSLLGMSASASASAGINGNITGERVAKTILENTLIYDFLDTVEFRKKKTIIDISEG
                     FKLNIQQDTMTYFATITPIASMFEGSKHLDEEELSFDVSKMNEAIRSIKGYFELIGTR
                     GNENRVFRFNIESFKNNYRIQDLKFMNLTLYSVLVGEISKENLNYDKEFDLNQGAKPS
                     GIGKFKGIRSEATKEDKYQVYDVILAGVK"
     gene            1000..>1373
                     /locus_tag="O0305_002839"
     CDS             1000..>1373
                     /locus_tag="O0305_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0275925.1"
                     /translation="MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTF
                     GTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQ
                     SLNSFHDSEIIEINYEYTTLKK"
BASE COUNT          539 a          157 c          251 g          426 t
ORIGIN      
        1 ggcaagcctg ctattgaatg gattatagat cagtacaacg taagcatcga caagaaatct
       61 ggaatattag atgatcctaa tgaatttagt gaagatccga attacatttt aaatctgctt
      121 ttatcagtta ttactgtaag tatgaaaact cttggattaa tagataaatt accagatttt
      181 gaagtattag attaaggaga ggatatattg acaaatacta tcaaaaaaat aatttatttt
      241 gatgaactat cagcaatgga tttattacag atagagaaga aaggtaacct tactaaaaca
      301 gttcagttga tgcatgatga aaaaggtagt gcaagtggtg aatctaaagt gaacatggaa
      361 tttggtaagc aaagcgctgt aaaacaaatt tttgattctt tacttgggat gtcagcgagt
      421 gcttcggcta gtgcaggtat aaatggaaat ataactggag agcgagtagc caaaacaatt
      481 ttagagaata cattaatata tgattttttg gatacagtag agtttagaaa gaaaaaaact
      541 attattgata tttcagaggg gtttaaacta aatattcaac aagatacgat gacatatttc
      601 gcgactatta cccccatagc aagtatgttt gaaggatcta aacatctaga cgaggaggag
      661 ctatcatttg atgtttcaaa aatgaatgaa gcgattagaa gtattaaagg ctattttgaa
      721 ttaataggaa caagagggaa tgaaaacaga gtttttagat ttaatataga atcttttaaa
      781 aataactata gaatacaaga tttaaaattt atgaatttaa cactctactc agtacttgtt
      841 ggtgaaataa gcaaagaaaa tctaaattac gataaagagt ttgatctaaa tcaaggtgcc
      901 aaacctagtg gaattggaaa gtttaaggga attagatcag aagcaactaa agaagataaa
      961 tatcaagtgt atgatgttat tcttgcagga gtgaaataaa tggaaattca tgtttatacg
     1021 ggaccgcttt gtggatttaa tgaatatatt aattctgaag ttaaagaata tcaccgcttc
     1081 atggaattaa tcagagaata caatatcata gtgcgagcaa gtgatacatt tggaactcat
     1141 gatgttgatg gtatttttga tactgagata gaaaatcttg tgatattttc aaacgacttt
     1201 gcttctgtaa caactcatgt tattactaat tttataaata tcgttacttt aggtagaaat
     1261 attaagaaaa tctatattca gaatcctcca aaaagagtat ttcaatcact aaatagtttt
     1321 cacgattctg aaataataga aataaactat gaatatacaa cattaaaaaa aga
//
LOCUS       DAKTIP010000348         1370 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.355, whole genome shotgun sequence.
ACCESSION   DAKTIP010000348 DAKTIP010000000
VERSION     DAKTIP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.355"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(221..1348)
                     /locus_tag="O0305_002840"
     CDS             complement(221..1348)
                     /locus_tag="O0305_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HCY0275926.1"
                     /translation="MKTRAAVLHEMGAKKPYKESKPLKIEYLELDNPSEHEVLIKIHA
                     AGLCHSDLSVINGNRPRPLPMALGHEASGEVIKVGKAVTRVSEGDHVVCTFIPSCGKC
                     IPCKEGRPALCENGAISNEKGEMLEGGMRLSNDEGKVYHHLGISGFAEYSVVSENSIV
                     KIDKKIPFERAAAFGCAIITGIGAVVNTAQIRSGSNVAVVGLGGIGLNAIIGAKLAGA
                     NEIIALDINEDKFELAKQFGATATFNSSDKDIDEQIKEYIPGGVEYAFETAGVVPAMK
                     VAYQITKRGGTTVTTGLPNPKDNFSFPQVTLAAEERTIKGSYVGSCVPDRDIPRFVNL
                     YNQGRLNIDSLISEVITLDEINEGFDRLSNGEVGRIIMKMH"
BASE COUNT          421 a          288 c          189 g          472 t
ORIGIN      
        1 cttttgcggt gaaggatgct cctatatctt tttttatcta aaaaaactat tagtaattag
       61 ttttaaaaca tttatcttct aatatataga tatgaaatgt aaaaatacaa ttagattggc
      121 agtcacaatg caaatgtaat atttgtcagt aatcttccta ctcaaattgt tattcttttc
      181 atagaaatca agaggaggaa cgacttgaat tctattatat tcaatgcatc ttcattataa
      241 ttctacctac ttcaccatta gacaaacgat caaatccctc attaatttca tctaaagtga
      301 taacctcact gatgagtgaa tcaatattta aacgtccttg gttgtataga ttaacgaacc
      361 ttggtatatc tctgtcaggt acacaacttc ctacatatga tcctttaatg gtacgttctt
      421 cagccgctaa agtaacttga gggaaagaaa aattatcttt aggattaggc agtcctgttg
      481 ttacagttgt cccccctcgt ttagtaatct gataagcaac tttcatagct ggcactacac
      541 ccgctgtttc aaaagcatat tctactccgc caggaatata ttctttaatt tgctcatcga
      601 tatctttatc gcttgaatta aatgttgctg tagccccaaa ttgctttgct aattcaaatt
      661 tatcttcgtt aatatctaaa gcaataattt cattggctcc cgctagttta gctccaataa
      721 tagcattcaa tccaatacct cctaaaccaa caaccgctac attactacca gaacgaattt
      781 gggctgtatt caccacagca ccgatacctg tgataatagc acaaccaaat gcagctgcac
      841 gttcaaaagg tattttttta tcaattttaa ctatagagtt ttcagagaca acagaatatt
      901 cagcaaaacc tgatatcccc aagtgatgat atacttttcc ttcatcatta gataaacgca
      961 tccctccctc taacatttcg cctttttcat tagatattgc tccgttttca cataatgcag
     1021 gacgtccttc tttacatggg atacattttc cacaactggg aataaatgtg cataccacat
     1081 gatctccttc gctaactctt gtaacagctt ttccaacttt aattacttca ccggaagctt
     1141 catgaccaag tgccataggt aaaggtctag gtctgttacc attaattact gacaaatcag
     1201 agtgacataa tccagctgca tgaattttaa ttaatacttc gtgttcgcta ggattatcaa
     1261 gttctaagta ttcaattttt aaaggtttag attctttata gggcttttta gcccccattt
     1321 catgtaaaac tgctgctcta gttttcactt ccaacactcc tttaatttat
//
LOCUS       DAKTIP010000349         1341 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.356, whole genome shotgun sequence.
ACCESSION   DAKTIP010000349 DAKTIP010000000
VERSION     DAKTIP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.356"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            37..642
                     /locus_tag="O0305_002841"
     CDS             37..642
                     /locus_tag="O0305_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0275927.1"
                     /translation="MITYSERHNIPIIEDDIFRDIFFSDPTPSIKTYDKLGKVIHISS
                     FSKTIAPAIRIGWIVASEKIIEQLADVRMQIDYGSSILSQMVVYEMLKNKSYDKHLVK
                     LRYVLKDKRDFMLNILNNLFKDIAHWEVPSGGYFVWLVFKIDIDIKYLFYELLSKEKI
                     LINPGYIYGSKEKSIRLSFAFESNENIKHALYKIYTYVKKV"
     gene            832..1305
                     /locus_tag="O0305_002842"
     CDS             832..1305
                     /locus_tag="O0305_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCY0275928.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
BASE COUNT          492 a          168 c          222 g          459 t
ORIGIN      
        1 acaggtcgtt ctttaacgaa tgagcaaaag aaaaatataa ttacttatag cgaaagacat
       61 aatattccta tcattgaaga tgatatcttt agagatattt tctttagcga tccaactcct
      121 tctatcaaaa cttatgataa attgggaaaa gttatacata taagcagttt ttcaaaaacg
      181 attgcaccag caataagaat aggttggatt gttgcttctg aaaaaataat agagcaattg
      241 gcagatgtaa gaatgcaaat tgactatgga tccagtattt tgtcacaaat ggttgtatat
      301 gagatgttga aaaataagtc ttatgataaa cacttagtaa agttaaggta tgttttaaaa
      361 gataaacgag actttatgtt aaacatcctc aataatttat ttaaggatat agcacattgg
      421 gaggttccaa gtggaggtta ttttgtatgg ttagtcttta aaatagatat agatattaaa
      481 tatttatttt acgaattgtt aagtaaagaa aaaatattaa tcaatccggg ttacatttat
      541 ggcagtaaag aaaagagtat aaggctatct tttgcctttg aatcaaatga aaatattaag
      601 catgcgctct ataaaattta tacatatgtg aaaaaggttt aattaaaaca ataattcgaa
      661 tcattatgtg gcatgttaaa cagctaaata taagctatgc acatttaaca agcggatact
      721 cctgacgcaa ttatagtatc attacatatt gtttaatcgt ttggggtaat gtaattaaaa
      781 ggtaaaatag gcagagtgtt atacataaaa tgtaaagaaa gaggcgatag aatgtctaaa
      841 atcaggtctt ttacaatatt aagtctactt atttacttag ctatgatgtg ctatacagta
      901 gtgacctatc caaaattacc aaccaaagtg cctattcatt ataatttagc aggggatgct
      961 gataattttg ctgataaatg ggtgctgctt ttgattaata gcgcatttat agtgatttgg
     1021 cttatatttt tcattgcagg tagatactat gaacgatttg ccaaatggtc acattataat
     1081 catacaccac gtgaaattcg agcgattaaa ttatttttaa gtacgttaaa tttagagatt
     1141 atgagctata tgtctatctt cacagtatta gaaatttggc aaatacaaca ccatcatcaa
     1201 ttcaatttac tatggtttaa tatgatattt atcattatca ttggtttgac gcttgtcata
     1261 ttttgtttac ttcctacaat tcataaaatg agagattctc aataaaaata taaactctca
     1321 tgatgcagtt ttgaagcgaa t
//
LOCUS       DAKTIP010000350         1338 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.357, whole genome shotgun sequence.
ACCESSION   DAKTIP010000350 DAKTIP010000000
VERSION     DAKTIP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.357"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..517)
                     /locus_tag="O0305_002843"
     CDS             complement(<1..517)
                     /locus_tag="O0305_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0275929.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLDTNKVVKTAKAEE
                     KMTDGQLWKKVKDSLHDSDIILSNEYETINVTYLLSNGYSSSVSAPGNDDGGHLTQSI
                     DFKGLKQIDLTKENVYDDFNKKLDAKNTWNSLTEKLKGLGLLQNGQKVSIYSSDSSSP
                     VSGKVGEGVTSG"
BASE COUNT          496 a          201 c          159 g          482 t
ORIGIN      
        1 caccactagt tactccttct cccactttac ccgagactgg agaagagcta tctgaagaat
       61 aaatagatac tttttggcca ttttgtagta aaccaagacc ttttaacttc tcggttagag
      121 aattccatgt atttttagca tctagctttt tgttaaagtc atcgtagaca ttttccttag
      181 ttaaatcaat ttgttttaat cctttaaagt ctatagattg tgttagatgg cctccatcat
      241 catttccagg agcagaaacg ctgctagaat atccattgct taataaataa gtaacgttga
      301 ttgtttcgta ctcgttactt aaaataatat cagaatcatg taatgaatct ttaacttttt
      361 tccataattg accatctgtc attttttctt ctgctttagc cgttttaaca actttatttg
      421 tatctaatcc taagtatgaa gaatgtaagc ctgttcctaa tgttgttaat actaaagcac
      481 ttgctactaa tgttttacct aaaaattttg tattcatttt tattgctcct ttttttatat
      541 tgtaaacgtt tacaatgaaa atataataat aattttttaa aagaacaatt aactaaatat
      601 caaaaatgta ttaactatct attaactaaa aaatagaata attttttaac atagttttgt
      661 tgttttgaat tataaaaact aaagtaaaaa tgttggatgt gaaagattgt tgatgagaat
      721 tttgagatgt agataaatat caaactagtc tataggtaaa aatttataat taatttgaaa
      781 agaatgtctc aaataatgtt tgaattagca ggtgtcatac taagtaatgt taagtaaaca
      841 ttttatactt tatcagaaca aaaaagacat gcatcaacat gtcagtgaaa ctaatccata
      901 ttatcttaac aacgaaattg aatcgaacct gaaaaacgta tttacaaatc gcaactactc
      961 tatccaaggc aaaatcgcaa aatctgatcc ccgaaataac atatctcgaa aaaaattcca
     1021 taattcaatc atcttcaata tttaacaata cattactagt tccaaatata ctcatacata
     1081 catgtttcat tgattgactg catattgtca taaataatag gggaactaat cgctaataat
     1141 gttgttaaac acaatacata gtaaagtgaa agattataaa tacatgaatt gttcaccatt
     1201 ccgccatata ttttcaatca cctaatttcc gaataaatat tacaatttta attcaataat
     1261 taatatttaa gaccaaaata atcctaaaaa gtcaatttta attgcgtgat attgagtttt
     1321 aagcatattt aaataaat
//
LOCUS       DAKTIP010000351         1328 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.358, whole genome shotgun sequence.
ACCESSION   DAKTIP010000351 DAKTIP010000000
VERSION     DAKTIP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.358"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..136
                     /locus_tag="O0305_002844"
     CDS             <1..136
                     /locus_tag="O0305_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCY0275930.1"
                     /translation="RVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTKKVK
                     "
     gene            144..629
                     /locus_tag="O0305_002845"
     CDS             144..629
                     /locus_tag="O0305_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCY0275931.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            637..>1328
                     /locus_tag="O0305_002846"
     CDS             637..>1328
                     /locus_tag="O0305_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCY0275932.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDMEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQ"
BASE COUNT          481 a          172 c          236 g          439 t
ORIGIN      
        1 tagagttgca caattagtat caattctttt aattttaata agtataagtt taattgtata
       61 tagaaggatt aagtataatc caccgttgta tagcaaagtt ggggcgcttc catggccaac
      121 aaaaaaagtg aagtagtgat agtttgagga aatttttatc aaaaacacat catcatacaa
      181 accctttatg gcgtgtatac cgtcttgtta aattttcgaa agtttttaag aatgtaatta
      241 tcattgaatt ttcgaaattt attccaagta tggtactgaa aagacatata tataaacaac
      301 ttttaaatat taatatcggt aatcaatcgt cgatagctta taaagtaatg ttagatattt
      361 tttacccaga actgattacg attggtagta acagtgttat tggttacaat gtaacaattt
      421 tgacgcatga agcattagtt gatgaatttc gttatggacc agtgacgata ggatctaaca
      481 ctttgattgg tgcaaatgct accattttac ccggtataac gattggtgac aatgtaaaag
      541 ttgcagctgg tacggttgtt tcaaaagata taccggataa tggatttgca tatggcaacc
      601 ctatgtatat aaaaatgatt aggaggtgac aattttatgg cgcaaaagaa taataatgta
      661 attccaatga cttttgatga tgcattttat cgtaaaatgg ctaaacagaa gtttaaacaa
      721 agagaatata aacgagctgc tgaatacttt gaaaaagtgt tagaattgtc acctgatgat
      781 atggaaattc aaattgatta tgcacaatgt ctagtgcaac ttggtattgc taaaaaagca
      841 gaacatttat tttatgacaa tattatttat aataggcatc tagaagatag cttttatgaa
      901 ttgagtcagc tcaacattga agttaacgaa ccaaacaagg cattcttgtt tggtattaat
      961 tatgttattg ttagcgacga ccaagattat agagatgaat tagatcaaat gtttgatgtg
     1021 aaatatcaaa gtgaagaaca aattgaactt gaagctcaat tgtttgtagt tcaaatacta
     1081 ttccaatatc ttttttctca aggtcgatta aaagatgcaa agaattatgt cttacatcaa
     1141 ccacaagaag ttcaagatca tcgtgtagta cgtaatttat tggcaatgtg ttatttatat
     1201 ctcggtgaat atgatacggc taaagcattg tacgaagcac tattacaaga ggatagtaca
     1261 gatatatatg cattatgcca ttatactttg ctactttata acactaagga aaatgaacaa
     1321 tatcaaaa
//
LOCUS       DAKTIP010000352         1324 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.359, whole genome shotgun sequence.
ACCESSION   DAKTIP010000352 DAKTIP010000000
VERSION     DAKTIP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.359"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>1324
                     /locus_tag="O0305_002847"
     CDS             <1..>1324
                     /locus_tag="O0305_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCY0275933.1"
                     /translation="KLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTTVEHAYQLLLDE
                     GYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDYAFNLDEIDTKH
                     FPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGVICDPNQIIIGS
                     STEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDNGINVDEVIKSQ
                     KNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQ
                     GLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIA
                     TFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMHFTITVKNGLTL
                     QECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHVDA"
BASE COUNT          508 a          200 c          197 g          419 t
ORIGIN      
        1 aaattaaaaa aacaaataat tgaaggtcaa tttaaagaag gcgataaatt ttattctaaa
       61 agacaattaa gcaaacattt atctattagc caaacaaccg tagagcatgc atatcaactt
      121 ctattagacg aaggttacat ttattcaaga cctcgctctg gatattttgt gtccgaaatt
      181 gaatctttaa caattttaaa caatcaacct atcccctctc tttttgatga tgattcatat
      241 aaaccaaaag cgagtgatga agcttatgat tatgctttta atttggatga aatagatacg
      301 aaacatttcc ctatagaact tttcagaaag tattccaaag atctttatga tacaaatcat
      361 ttaaatcaat tacgtagagg acattttcaa ggagaattac atttgcggtt tcaactggca
      421 ttttatttat ttactaatcg tggcgtaatt tgtgatccta atcaaattat aattggctca
      481 tctaccgaac aattagtaaa tcaattagtc gatttacttt atacttcaac atttattatc
      541 gaaaaaccta gttatccacc aatcaaaaat attttagata aaaaacaagt tgaatatgaa
      601 caaattgaag ttgaagataa tggaataaac gtagatgaag ttattaaatc acaaaaaaat
      661 atagtatata taacgccttc ccatcaattt ccaactggat atgtgatgga cttaaaaaaa
      721 cgaactcaat taatacaatg ggctcaagaa aaagaagaac gatttattat agaagatgat
      781 tatgattccg agtttagata tttcggcaaa cccattccag caattcaagg tctatattca
      841 agaggagaaa aagtgattta tatcagtaca ttttcaaagt ctatttttcc tagctgtcga
      901 gttgcatata tggtgttacc ctattctata atgaaaaaat atcattctca aaatcacatt
      961 gaaggcaaca ctgtacctgt tcatatgcaa aaccttattg caacgtttat atcttccggt
     1021 ggttttgaaa ggcatttaaa taaaatgaga cgaatttatc gtagaaaact aacctatatt
     1081 ttaaaaagat tgaaacctta caaagagcag cttgatattc aaggtgctga aacgggtatg
     1141 cattttacaa ttacagtaaa aaatggatta acattacaag agtgtcttga tagagcaaac
     1201 aaagtaaagt taaaattaca agtttataat tttgatgatg atcaagacta taagaaaacc
     1261 cctaaattta tattaggctt tggcggtata gacgatgatg ctttaaaacc gcatgtagac
     1321 gctc
//
LOCUS       DAKTIP010000353         1324 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.360, whole genome shotgun sequence.
ACCESSION   DAKTIP010000353 DAKTIP010000000
VERSION     DAKTIP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.360"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            7..1248
                     /locus_tag="O0305_002848"
     CDS             7..1248
                     /locus_tag="O0305_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCY0275934.1"
                     /translation="MQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQLKVHIK
                     EDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSLNQGWS
                     KISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKNDYPSGY
                     SHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDEIQEDS
                     EIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGKVGEKL
                     ALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEKFIEIK
                     TSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYSEFKDV
                     DDLIDKVFNVEAIQYKVFGEK"
BASE COUNT          562 a          150 c          223 g          389 t
ORIGIN      
        1 aactttatgc aggtccttaa attaattaaa gagaaacgta ccaataatgt agttaaaaaa
       61 tctgattggg ataaaggtga tctatataaa actttagtcc atgataagtt acccaagcag
      121 ttaaaagtgc atataaaaga agataaatat tcagttgtag ggaaggttgc tactgggaac
      181 tatagtaaag ttccttggat ttcaatatat gatgagaata taacaaaaga aacaaaggat
      241 ggatattatt tggtatatct ttttcatccg gaaggagaag gcatatactt atctttgaat
      301 caaggatggt caaagataag tgatatgttt ccgcgggata aaaatgctgc aaaacaaaga
      361 gcattaactt tatcttccga actcaataaa tatattacat caaatgaatt taatactgga
      421 agattttatt acgcagaaaa taaagattca tcttatgatt taaaaaatga ttatccatca
      481 ggatattctc atggatcaat aagattcaaa tattatgatt tgaatgaagg attcacagaa
      541 gaagatatgc tagaggattt aaagaaattt ttagaactat ttaatgaatt agcttcaaaa
      601 gttacaaaaa catcctatga tagcttggtc aatagcatag acgaaataca ggaagacagc
      661 gaaattgaag aaattagaac agcacaaaaa gataagacac tcaaggaagt ggaagcacct
      721 aaaggaataa ttccaaaata taaaaaaggt gtatcaaaga ctactaaaaa tgattcagaa
      781 attgaaaaat caaataaaga gaataaatta accggtaaag ttggagaaaa attagcgcta
      841 aattacttta atgagctaat tgataataaa atagacgaag ataagaaaga acagtttagg
      901 aatattttaa atgataatcc aggctctcaa cacggtcatg gctatgattt agtagctttt
      961 gatccaacaa atacagataa agctgtagaa aaatttattg aaattaaaac atctacatct
     1021 tctagtattg aggaaccatt ttttatgtcg ctaaatgaaa tgtttgctat gaaagaatat
     1081 aagcagaaat atttaatatt aagaatattt aatgtttccg gtaaagaacc acaattttat
     1141 tttatagatc catatgcaaa ttattctgaa tttaaagatg tagatgatct cattgacaaa
     1201 gtatttaatg tagaagctat tcagtataaa gtttttggcg aaaaatgatt acttgaacaa
     1261 gagctaaaat aaaattgtga tctaataaaa atagaaggtt ctgttgcaaa gtaaaaaaat
     1321 atag
//
LOCUS       DAKTIP010000354         1307 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.361, whole genome shotgun sequence.
ACCESSION   DAKTIP010000354 DAKTIP010000000
VERSION     DAKTIP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.361"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..328
                     /locus_tag="O0305_002849"
     CDS             <1..328
                     /locus_tag="O0305_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCY0275935.1"
                     /translation="LLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEM
                     IKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSY
                     GNASGL"
     gene            385..>1307
                     /gene="holA"
                     /locus_tag="O0305_002850"
     CDS             385..>1307
                     /gene="holA"
                     /locus_tag="O0305_002850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCY0275936.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKS"
BASE COUNT          547 a          142 c          226 g          392 t
ORIGIN      
        1 tttattaatg ggcgatgcta gtaaaaataa tgaatcttta ctactaaaaa aatataactt
       61 gccggagatt gatattttaa aagtaggaca tcatgggagc aagacaagta gttctaaaga
      121 atttatagag atgattaagc ctaaaataag tttgatttct tctgggaaga acaatatgta
      181 tcatcttcct aatatagaag ttgttaaacg attgcaaagg attcgcagtc gcatttacaa
      241 tagtcaacaa aacggtcaag ttacaattga cttagatgat aatttaaaag ttgattcaag
      301 ctcttatgga aatgcaagtg gtttatagat acagaaacat gatataatct attgatgtgt
      361 caaacgaatg aaagggtgta atgaatgagc gacaatattg tagctattta tggagatgtg
      421 cctgaattgg ttgaaaaaca aagtgcagaa atcatatcac aatttttgaa aagtgataga
      481 gatgacttta actttgtgaa atataattta tacgaaacag agattgcacc aattgttgaa
      541 gaaacattaa cattgccttt cttttcagat aaaaaagcaa ttttggttaa aaatgcatat
      601 atatttacag gtgaaaaagc gccaaaagat atggctcata atgtagatca attaatagaa
      661 tttattgaaa aatatgatgg cgaaaatttg attgtctttg agatatatca aaataaactt
      721 gatgaaagaa aaaagttaac taaaactcta aaaaagcatg caaggcttaa aaaaatagag
      781 caaatgtctg aagaagaaat aaaaaaatgg attcaaagta aattaaatga gaatttcaaa
      841 gatatcaaaa gagatgcatt agatttattt attgagttga caggtattaa ctttaatatt
      901 gtctcacaag agatagaaaa gttgatttta tttttaggcg atagaccaac aattaataag
      961 caggatgtta accaaattat taatagaagt ttagagcaaa atgtattttt actgactgaa
     1021 tacattcaga aaagaaagaa agaacaagca attcatttag taaaagattt aataactatg
     1081 aaagaagaac caattaaatt acttgcacta attacaagta attaccgatt attttatcaa
     1141 tgtaagattc tgagtcaaaa aggatatagt ggacagcaaa ttgctaaaac aataggagta
     1201 catccataca gagtaaaatt agcgttagga caagtaagac attatcaact tgatgaatta
     1261 ttgaatatta tagatgcttg tgcggaaact gattataaac ttaaatc
//
LOCUS       DAKTIP010000355         1289 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.362, whole genome shotgun sequence.
ACCESSION   DAKTIP010000355 DAKTIP010000000
VERSION     DAKTIP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.362"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            384..1184
                     /locus_tag="O0305_002851"
     CDS             384..1184
                     /locus_tag="O0305_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCY0275937.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKLRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYNGNELYNYKSFNRQVFQQVVDYLNMKQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGNDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLKK"
BASE COUNT          455 a          159 c          245 g          430 t
ORIGIN      
        1 taaatcacaa ttgtcaacta gttgtttgtt tttaactttt aaagaatagt tatttagttt
       61 catgtataca actcctatgt ataaagggat ttattacacc gatatttaat tgtattttta
      121 aaaattcttt cacattatgt atagatgtta taaattagta tatcacacta tattttgtct
      181 aaatgataaa tatagtgtga tatttttaca atattctgaa atttatgttc gcctctgaat
      241 gctatatcca gtgtaatgtg ttttgcatat atgaaagcaa tttcaaaatg tgaatatagg
      301 ttcattgtgg tatgacaaac ttcattgctt gtcatgagat ggatataatg aagacgacta
      361 aaagagcaat gaggaggata atcatggtta aggctattgc ggtagatatg gacgggacgt
      421 ttttagattc aaaaaagaca tatgataaac ttagatttga agcgattttt actgaactta
      481 gaaatagaga tattacattt attgctgcga gtggcaatca atatgcgaag ttgaagtcta
      541 ttttcgggga tagagatatg tattttattt ctgaaaatgg tgcagttatt tataatggca
      601 atgagttata taattataaa agctttaatc gtcaggtgtt tcaacaggtt gtcgattact
      661 taaatatgaa gcaaagtatt gatcaactcg tcatctgtgg tttgaaaagc gcgtatattt
      721 taaaacatac ttctgaagcg tttaaagaag atacgagatt ttattatcat cagttaaaag
      781 aaattgacag tctacagcaa ttacctgagg atgattatgt caaaatagca tttaatatta
      841 atcgtgagac gcatccgaat gttgacgaag aagtagcaac gcaattcagc aatgatatta
      901 aacttgtctc aagtgggaac gatagcattg atattattat gccaaacatg actaaagggc
      961 aggcattaaa acgattgtta gataaatggg aaatgtctcc ttcagagctc atggccttcg
     1021 gagatgcgaa taatgataaa gatatgttag cgttcgctaa gcatagttat gtgatggaaa
     1081 atagtcatga tgaagaattg ttcaatattg cctctgctgt cgctccaagt aatgataagc
     1141 agggtgtatt aacgattatc gaacaagaag tgttgaaaaa gtaggtagta tatcatcaat
     1201 gaagcagcta tcatgatact aaatacatgt taaatgaaaa ctttgaatac gaatcatcaa
     1261 atattaatat atctcaaaca ggttaggca
//
LOCUS       DAKTIP010000356         1285 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.363, whole genome shotgun sequence.
ACCESSION   DAKTIP010000356 DAKTIP010000000
VERSION     DAKTIP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.363"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1125)
                     /locus_tag="O0305_002852"
     CDS             complement(<1..1125)
                     /locus_tag="O0305_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0275938.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISIIIPLLSLSNIF"
BASE COUNT          495 a          220 c          159 g          411 t
ORIGIN      
        1 aaatatatta cttaagctta gtaaaggtat aattatactt atcagtccta aagacaatgt
       61 atccgctgca taattcgatg tagatgagat actaaatact ttccccatca aatgatttgg
      121 cgtttttatt tgaatggcaa ctgatcttgt tagtccctct atagattgtc caagtcccaa
      181 taatgttgca cctatatata atatcgccac acttggaaac acattaataa tcgataagcc
      241 aattccccaa actaaaacac caatactaaa tttaaagatt aatcgctttt ctgacagcaa
      301 acccataatc aatgacatta ataaagatgc tatacctaaa catgatgtag ctagtccata
      361 tacaccaacg ccctctttta atatattgga aataaacaat ggtaatacaa cacgccaaag
      421 gccagtatta atcaatatgc aggcaaattg gatgattata ataaatggaa tttctttaga
      481 ttgtttcaag aattcccaag tttcagaaaa atcttctttt gagtgtctat caatcatgtt
      541 gttatttgta tattttaaaa gtgcattaaa aataaatcct aaaaatagca atatactaca
      601 aataaaaaag acgccaacat taccaactag tattacaatg acaccaatta aagcaggtaa
      661 aataatattt gagcctcttt gcaaactatc gattaacgca ttacttgttg ctaaatgctc
      721 ctcatcaata atttcaggaa gaattgccct aaacgcagga tccgtatagc agttaataat
      781 ggtgatagct gtagatatgg ttagaagcgt cagataattt aaatttgatg ttattgcaag
      841 taaaggaata attattataa tcaaacttag tataagatca gatagataaa gtattttctt
      901 tttattatgt ttatcagaat atgcgccacc gaaaatacca aatataataa atggaagtgt
      961 ttgactcata accatcattg ataattttaa agatgattgg tttgtcaatt caacagtaaa
     1021 ccaaattatt tgtaacgaaa acagcacaaa acaactccga cgtaagaaat taccaatcaa
     1081 taaatatgta aagtttctat ttttcaaaac ttctaaatac aacatattta tcacctctca
     1141 taaaaataat tgaatgcatc caccagcttt tttagacctt cttctaaact ctctttatcc
     1201 aaagcgcaat taattctaat ataatttagt cagttaaata tcaattattt cgaaatatac
     1261 atactacttg aaacaccata cataa
//
LOCUS       DAKTIP010000357         1277 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.364, whole genome shotgun sequence.
ACCESSION   DAKTIP010000357 DAKTIP010000000
VERSION     DAKTIP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.364"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            67..1026
                     /gene="hyl"
                     /locus_tag="O0305_002853"
     CDS             67..1026
                     /gene="hyl"
                     /locus_tag="O0305_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCY0275939.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN"
BASE COUNT          483 a          184 c          213 g          397 t
ORIGIN      
        1 tattctaaat tgacatattg attattgttt cctcaaatcg ttttaaaaaa tagaaggatg
       61 atgaaaatga aaacacgtat agtcagctca gtaacaacaa cactattgct aggttccata
      121 ttaatgaatc ctgtcgctaa tgccgcagat tctgatatta atattaaaac cggtactaca
      181 gatattggaa gcaatactac agtaaaaaca ggtgatttag tcacttatga taaagaaaat
      241 ggcatgcaca aaaaagtatt ttatagtttt atcgatgata aaaatcataa taaaaaactg
      301 ctagttatta gaacgaaagg taccattgct ggtcaatata gagtttatag cgaagaaggt
      361 gctaacaaaa gtggtttagc ctggccttca gcctttaagg tacagttgca actacctgat
      421 aatgaagtag ctcaaatatc tgattactat ccaagaaatt cgattgatac aaaagagtat
      481 atgagtactt taacttatgg attcaacggt aatgttactg gtgatgatac aggaaaaatt
      541 ggcggcctta ttggtgcaaa tgtttcgatt ggtcatacac tgaaatatgt tcaacctgat
      601 ttcaaaacaa ttttagagag cccaactgat aaaaaagtag gctggaaagt gatatttaac
      661 aatatggtga atcaaaattg gggaccatat gatagagatt cttggaaccc ggtatatggc
      721 aatcaacttt tcatgaaaac tagaaatggc tctatgaaag cagcagataa cttccttgat
      781 cctaacaaag caagttctct attatcttca gggttttcac cagacttcgc tacagttatt
      841 actatggata gaaaagcatc caaacaacaa acaaatatag atgtaatata cgaacgagtt
      901 cgtgatgact accaattgca ctggacttca acaaattgga aaggtaccaa tactaaagat
      961 aaatggatag atcgttcttc agaaagatat aaaatcgatt gggaaaaaga agaaatgaca
     1021 aattaatgta aattatttgt tcatgtacaa ataaatataa tttataactt tagccgataa
     1081 cttcagaaat gatgtttttc ggctaatttt tatactgaat ttagtttaat taacttatga
     1141 tatttttaat ttaaactatc tacttgattt gctttcctga cttaaaaatt agactaaaca
     1201 tgacactata tgaggtaatt tttaaaataa gaaatttgaa ttccatgttg tcaaaccctt
     1261 tatataaaag ttttgaa
//
LOCUS       DAKTIP010000358         1276 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.365, whole genome shotgun sequence.
ACCESSION   DAKTIP010000358 DAKTIP010000000
VERSION     DAKTIP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.365"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..807
                     /gene="hlgA"
                     /locus_tag="O0305_002854"
     CDS             <1..807
                     /gene="hlgA"
                     /locus_tag="O0305_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCY0275940.1"
                     /translation="IIKRTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISS
                     RTTYSDLKKYPYIKRMIWPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIG
                     GNFQSAPSIGGSGSFNYSKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSA
                     YDQYLFAQDPTGPAARDYFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYG
                     RNMDATYAYVTRHRLAVDRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT          522 a          204 c          177 g          373 t
ORIGIN      
        1 atcatcaaaa gaacacaaga cattactagc aaacgattag ctataactca aaacattcaa
       61 tttgattttg taaaagataa aaaatataac aaagatgccc tagttgttaa gatgcaaggc
      121 ttcattagct ctagaacaac atattcagac ttaaaaaaat atccatatat taaaagaatg
      181 atatggccat ttcaatataa tatcagtttg aaaacgaaag actctaatgt tgatttaatt
      241 aattatcttc ctaaaaataa aattgattca gcagatgtta gtcagaaatt aggctataat
      301 atcggcggaa acttccaatc agcgccatca atcggaggca gtggctcatt caactactct
      361 aaaacaatta gttataatca aaaaaactat gttactgaag tagaaagtca gaactctaaa
      421 ggtgttaaat ggggagtgaa agcaaattca ttcgttacac cgaatggtca agtatctgca
      481 tatgatcaat acttatttgc acaagaccca actggtccag cagcacgaga ctatttcgtc
      541 ccagataatc aactacctcc tttaattcaa agtggcttta atccatcatt tattacaaca
      601 ttgtcacacg aaagaggtaa aggtgataaa agcgagtttg aaatcactta cggcagaaac
      661 atggatgcta catatgctta cgtgacaaga catcgtttag ccgttgatag aaaacatgat
      721 gcttttaaaa accgaaacgt tacagttaaa tatgaagtga actggaaaac acatgaagta
      781 aaaattaaaa gcatcacacc taagtaaaca gttcaatcat cttaaaaaat cctgggacac
      841 ttcatacttg tctcaggatt ttttaacaaa ttgaatcagc cttataacat taaattattt
      901 tatcgtacat taaatttaat aataacaact gatttttata agaataaagt atcgaaccat
      961 agtagataca caaataatac aaatgaaaca atttaacttg aaagcttaaa taaatattat
     1021 caagttaata aacaattaat ttttagatgg attcatcaaa aatcgtaaaa agcacaattt
     1081 gtattttaca aacattaatt aaaaaagaaa gcaagacatt cgtgcaatcg gttaccttaa
     1141 attgtttaca actgtcaaca ataccaaggt tttattaact atatttctca caaaattagc
     1201 ttttagcatt ccaaacaaaa aaggttaaat cgaacggaat tatggcattt ttaacttaat
     1261 tgtaaaaaaa gttgat
//
LOCUS       DAKTIP010000359         1269 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.366, whole genome shotgun sequence.
ACCESSION   DAKTIP010000359 DAKTIP010000000
VERSION     DAKTIP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.366"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(127..819)
                     /locus_tag="O0305_002855"
     CDS             complement(127..819)
                     /locus_tag="O0305_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCY0275941.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFADVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1055..1269
                     /locus_tag="O0305_002856"
                     /pseudo
     CDS             1055..1269
                     /locus_tag="O0305_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          422 a          231 c          177 g          439 t
ORIGIN      
        1 tctttaaaaa tataaatacc acgacatatt gctttaacat tttcatttat aaagcgaaaa
       61 aatgcatcgc tactaagttg aatgtttagt aaggatgcat ttaattcact aaaatgatta
      121 aattacttat atcttttcat ctgattgatt atcgaaattt cttccttcta aacctgctaa
      181 ctcttcttta gaagctgcag gtgctttcat ttcaaatatc tcattcacta ctgtgtaatc
      241 gtaatatcct aatctggcaa taggtttaat cgacttaatg tccaatttac cattatcaag
      301 aataacctta tcgtcaatat gaacttgggc aactcttcct ataacaatat ctacggtaga
      361 tactggatct ccagttggaa tacgaatcgt ttgaacgtac tcacattcaa aatgaactgg
      421 cgattctttt acacgatatc ctggagcttc tatacatttt tcctttgtta catctgcaaa
      481 attaaattca tcctcttctg gtggcaatgc tttcgatgat aaattaactg cttctcttaa
      541 atcatacgtt gccatattcc acacaaacca acctgtctct tcagcatttt tcactgtatc
      601 tttacgttcg tgatcaccaa gaacggattg atttgctgcg aacataacca taggcggatc
      661 ccaagttaag ttttgatact gactataagg cgctaaatta tctttcccat ctttcgatac
      721 agtagagatc caccctattg gacgtggtac tgtactactt ttaaatgggt cgtgcggtaa
      781 accatgactt cttacacctt gttttggcga ataattcata ctatcttcac cccttataag
      841 taattacatt taaggttacg ccctctttac ataagcgtct aatataaata aacaatttat
      901 ttataagtag aaactatata tgacgtggtt gcttataatt tgcgttcttg attcgaaaaa
      961 ttcagataag gatttataca attaatattt atgatatctt ttgtaaattt aattaattat
     1021 agttacttca atcatgatta gtttataata ataaagtgaa attgaaaaag acagctatta
     1081 tgcgatgagc gaaaaacttc aagtaaaaca agaatttaac ggcgttacaa ggcaatactt
     1141 gaatttcaga acagatagta tcgatgatct ggataagttc aaaaaagaat tcaaacctgt
     1201 tatgcactta aaaggtgatg cattcaatca acagttacaa tctttgatta acaaatatcc
     1261 acaaataca
//
LOCUS       DAKTIP010000360         1268 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.367, whole genome shotgun sequence.
ACCESSION   DAKTIP010000360 DAKTIP010000000
VERSION     DAKTIP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.367"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            97..204
                     /locus_tag="O0305_002857"
                     /pseudo
     CDS             97..204
                     /locus_tag="O0305_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            316..1110
                     /locus_tag="O0305_002858"
     CDS             316..1110
                     /locus_tag="O0305_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0275942.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTKTNGRNAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNT
                     RTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLKKEIENFKFFV
                     QYGNFKDINDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDIPTKQAPKLLLK
                     GDGDLKGSSVGSRSLEFTFVENKEENIYFTDSVQYTPSEDTRYESN"
     gene            complement(1114..1236)
                     /locus_tag="O0305_002859"
     CDS             complement(1114..1236)
                     /locus_tag="O0305_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275943.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
BASE COUNT          516 a          155 c          226 g          371 t
ORIGIN      
        1 gttttaacga attttgatgt ttcttgaact atatatcatc tagtcgtcat ttacaattgg
       61 taaatatgac ttcaaactgt atgaaggtga tggcgattaa aaggctcatc cgtaggttct
      121 aaagaactag aatttaactt tataagaaat aaagaagaaa atgtgtattt ttctgatagt
      181 attaatttca aacctactga atagggttaa tagaagtttg aaataaaaat ttcataaagt
      241 gttcaaaata atttttcgct ttagttgtaa atattaaaaa ctgagtcaat attaaaaata
      301 taaggtggag aaatgatgat tcattcaaag aaattaacgc tcggtatatg cttggtatta
      361 ctcattatat tgattgtagg ttatgtcatt atgacaaaaa caaatggtcg aaacgcccaa
      421 attaaagaca catttaatca aacattaaaa ttatatccaa ccaaaaatct cgatgatttt
      481 tacgataaag aaggctttcg agatcaagaa tttaaaaagg gtgataaagg tacttggata
      541 gttaattctg aaatggtaat cgagccaaaa ggtaaggata tggaaacgag aggaatggtg
      601 ctctatatca atcgcaatac tagaaccaca aaagggtatt attttataag tgaaatgaca
      661 gatgacagta acggcagacc aaaggatgat gaaaaaaggt atccggtaaa aatggaacat
      721 aataaaatca taccaacgaa gccactaccg aatgacaagt taaaaaaaga gattgaaaac
      781 tttaagttct ttgtacaata tggcaacttt aaagatatta atgattataa agatggtgat
      841 atttcatata atcctaatgt accaagttat tcggcaaaat atcaattgaa taatgatgat
      901 tataatgtcc aacagttaag aaaaagatat gatattccaa ccaaacaagc gccgaaacta
      961 ttattgaaag gcgatggaga tttaaaaggt tcatccgtag gttctagaag tcttgaattt
     1021 acctttgtcg aaaataaaga agaaaacatt tattttacag attctgtaca atatactcca
     1081 agtgaggata caaggtatga gtcaaactga atatcaaata aaacctggta atataacaag
     1141 taactctgaa gaaacaagtt cgatatctaa agtgagctgt gaaatatagg tagccatttc
     1201 aaaaaattta aaggtgaatt tgataatgta gctcaaggag attgggttaa aaaggcgaag
     1261 aatgaact
//
LOCUS       DAKTIP010000361         1267 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.368, whole genome shotgun sequence.
ACCESSION   DAKTIP010000361 DAKTIP010000000
VERSION     DAKTIP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.368"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(107..1261)
                     /locus_tag="O0305_002860"
     CDS             complement(107..1261)
                     /locus_tag="O0305_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCY0275944.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
BASE COUNT          375 a          278 c          154 g          460 t
ORIGIN      
        1 aacaaaatat cttaacatcc ctataaatcc ccacaaaaaa agggtaaatc cgaacgtcaa
       61 atttcatctc tttatgccaa tcaacgatcg atttaccttt taaaatttaa aatgtatctg
      121 taatactctt agcaatattt gtatatgctg catcttccat aggtggattg tgtaatcctg
      181 cacgcatgtc acgatagtaa cgttgtaatg gtcgattcat ttctaagctt ttagcaccaa
      241 ctattctcat cgctaaatca atcacttcaa gcccttgatt cattaccatc actttactcg
      301 cacttgttgg atttcttatc tgactgtcat ctttatattg ttgataccct tttgctgtac
      361 tccataaaaa ttgtctcgca cataacaata gcgtttccat tttccctaaa ttttgttgca
      421 cagttggcaa tgttgcaatc gttccttcaa tactattagg gctatgttgg attgcaaagt
      481 ctacagcata atttctagca gcttgtgcta tacctaaata acagcttggt atatgcaata
      541 tccaaccatt tggcgcttta cttttctctg tttccactaa atgttttaat ggtactttta
      601 catcatttaa tattaaatca tgactttctg tcgctcgcat acctaatacg tcccaattat
      661 cggcaatttc aacaccaggc aaattcctgt ctactaagaa aaaaccaaca ctttctaatt
      721 cttctatata agcagcaaca ataatatgtg ttagtgcttt actcattgat gtatatgtct
      781 tcacaccatt taaaatatac ccatcatcag ctttaacagc atgtgtactt ggtcttcccc
      841 ctcttgttgg actacccatt tcagcttcac taactgctct attaactaat gcaccattat
      901 taatttcaac agcaaattgc tccaacatat cttgagacca taatttctgt tcataaattt
      961 gtcctacgac actcacatgc caaccaatag ataatgctgt ggcaccatca agttcgccta
     1021 aaaatgattg caaaataacc atgtcttcta tggtcgcacc ttcaccacca tatgcttttg
     1081 gtaacgttaa ttttccataa ccttctttaa ttaaccattc aatattttta tatggaaatt
     1141 ggctatgttg atcattatat tcagctttag ccttaaatgt atctctaatt actttgaatt
     1201 tttcaatcca ttttctttga atttccgttg ttattaaaaa ggaattatcc atatcattca
     1261 tattagt
//
LOCUS       DAKTIP010000362         1256 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.369, whole genome shotgun sequence.
ACCESSION   DAKTIP010000362 DAKTIP010000000
VERSION     DAKTIP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.369"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(207..1193)
                     /locus_tag="O0305_002861"
     CDS             complement(207..1193)
                     /locus_tag="O0305_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCY0275945.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT          396 a          239 c          137 g          484 t
ORIGIN      
        1 ctttttaaaa tgatttgttt ttgttaatta catatattta tcaatttata acactttttc
       61 actttaatat tatcaaaaat ttcacaaaca atctgttcaa tttataatat tgttgaatta
      121 ttacttattt tcaaaataaa aacgagtagt gaaaataatt agaatctaac tattttcata
      181 ctcgtcttaa tatcattatt gaatccttat ttattactat ctataatttc acttacgata
      241 tcatcatatc caggccacgt atatcctgca tagtcaacaa tcagccaacc cgttctagct
      301 ttcccatttg ctttaatcgt ttttgcaatt tcaggattta tatgtgatgc atagttataa
      361 gtactattaa atgcgctgcc tccagacgct acactcaaga aattcacata tacattgtct
      421 ttgttactat ccgttttagc tttagccaat aaatttttaa cagcttcaac ttttttatca
      481 tagtaatctt tatactcatc ttgtactttt aaatttaagc taccattatt aattttcgtt
      541 tcaaatgtcg cattgtctgc ccattgaata cctgacgtgt cagcaccata accacttttt
      601 atgtacgtac cccccattct attgaatagg acaattttac ctttcgtttc ttttaaagta
      661 ggattcgcat tacttcctgt gtaaaaaaga ttctgatatt gcgggttatt ataataatat
      721 tctctaaaaa tttcttcaaa tgtcttcgta actttagaat cgctatcgta gtcctttttc
      781 atagacatca caattgtttc gtttggataa gcactcaagt aatatttagc atcatcgaga
      841 aattttccta attcatgatg caaataaacc atgccgtgat gaactgaaat catattatca
      901 gcacttgctc tacctctaat atcaaaaaac ctgactcccg acttcatttg ggtaaggtaa
      961 tctttatctt gagtctttgc ccaaactgat tttactggat cctttaaagt gaatgagcca
     1021 ctatcatgtg aacccggtat attaatctca gttaaatgtt ttccatcatc aagtttactc
     1081 atccaatttt ctggactttt actcaacgaa tctgacgcat gtgctgaatg ataccaacca
     1141 ctcatcacta ctaaaatgat acttaaaaac aaagtcttaa tacacttttt cataatatca
     1201 acttctttct atatttaata cattaattat acatcttttt taaataaaaa tatgtg
//
LOCUS       DAKTIP010000363         1246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.370, whole genome shotgun sequence.
ACCESSION   DAKTIP010000363 DAKTIP010000000
VERSION     DAKTIP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.370"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(177..780)
                     /locus_tag="O0305_002862"
                     /pseudo
     CDS             complement(177..780)
                     /locus_tag="O0305_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          458 a          203 c          150 g          435 t
ORIGIN      
        1 atattatcat taccatattt attcaacaaa tgtttgtaaa atcctcacta ataaaattaa
       61 tcgatatgac cataattcat tatccaataa atgactataa aaatttcttc tatgccttca
      121 tttgcaacta aatttggaca tcaaaaaaga gcactcctga atgagcactc ccttaattat
      181 ctttccgtta tatttgtatt gtcgacggtt tcgatttctt tatttttatt tgtagctttt
      241 ttcgcaacaa ttaacaacgt tgaaagtacc gctaaagcta aataagcata accgcccgct
      301 tgtttcatta caaaactacc gtaatagtat cttacgccac cttcgtccat ataaattttc
      361 gtagtgtgaa ccaaaactag agataagcct agacaaattg acaaaccaat cgtagtgtaa
      421 accatgtatt ttttaaatgt ctgttttttc tttttttgct tataaaacgg tagaaaacat
      481 aatccatatg gcattaaaaa aatgatacct gcaatgccaa aggtaagtat gacaagccaa
      541 aaaagaaacg ctgatactat ataatataga taaaattgag aaataccagc cgtagccaag
      601 attagaaata aaaaagtagt agcgaattta tatgtctcac ttactttatt aacttggtta
      661 aaaaagttat caattgcttt attctttttt tgaaatatgt actatacctt ctataatcat
      721 ccaaataata ccaccaataa ctaacaaagc aaaaactggg attacaagac taattaacat
      781 atcgttcacc tgctcacctc tattttcaaa caattatgat tgaattgaat cattctaatt
      841 tttgtaaagt tacttgttta atttattata tttattaact ctatgataac aaaaatactg
      901 tttgtgtata acaataaatt gagatactca ataatttgat aatgtgaaat tgatactttt
      961 caaaatagtt gaatatgaca attaatgatt agttttgcaa tggtgctata atcaatattt
     1021 agcagttatc attatgcttg aaagggtggt aaaggcggag aagtcatcca ctttttccta
     1081 tactacaagg acctttactt taaaacaacg aatttacaaa ataaaaggca taatttcttt
     1141 tctgtcacat cgctcattct aactactaga cttttaaaat cgttcctgta atttaaactc
     1201 aatcaacttt caattaatga tgcataatat gattataagt atagta
//
LOCUS       DAKTIP010000364         1246 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.371, whole genome shotgun sequence.
ACCESSION   DAKTIP010000364 DAKTIP010000000
VERSION     DAKTIP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.371"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..1178
                     /locus_tag="O0305_002863"
     CDS             <1..1178
                     /locus_tag="O0305_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCY0275946.1"
                     /translation="LASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSADSESK
                     GGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGDAVGQS
                     QAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDA
                     RFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKMAETKK
                     GVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGPRIMAL
                     EPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSR
                     KFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL"
BASE COUNT          474 a          169 c          242 g          361 t
ORIGIN      
        1 cattagctag tccagagttt gatgatttaa agagaactga tgcaagtcaa gtcgatgggc
       61 aagatgatac ttcagaagat gatgataacg aaagtgaaaa agctgactct aaatctgcag
      121 atagtgagtc aaaaggtggc gcctatttag aaatggagct tcacgaagga caaaatagtg
      181 aaacattagg taatgatgaa gcacgtgaag gcgatgctac agatgatatg actgatatga
      241 tgaccaaaaa gggcaaagga tcaaacgata cgctaaatcg tgaagaaggc gatgctgttg
      301 gtcaaagcca agcatttcaa cttgatggtg tgaacaaaaa tgtagaaata aaatggcaaa
      361 ttcctgaaat tgagccacaa tatgttttag aatatcaaga atcgaaacaa gacgtacaat
      421 atgaaatcaa agacttaatt caaattataa agaaaacgat tgaacgagaa caacgagatg
      481 cacgttttaa tttaacaaaa gggcgattac aaaaggattt aattaattgg tttatcgacg
      541 atcaatataa attgttttac aaaaaacaag atttaagtaa atcatttgat gcgacattta
      601 cattgttaat tgatgcgtca gcaagtatgc atgataaaat ggctgaaacg aagaaaggtg
      661 tagtgttatt ccacgagacg ttaaaagcat tgaacattaa acatgaaatt ttatcattca
      721 gtgaggatgc ctttgattca gacgagcatg cacaaccaaa tatcattaat gaaattatta
      781 actatgatta ctcaaccttt gaaaaagatg gcccacgtat tatggcactt gaacctcaag
      841 atgataatcg tgatggtgtt gctattagag ttgcaagtga acgattaatg cgtcgaaatc
      901 aacatcaacg atttttaatc gtattttcag acggcgaacc gtctgcattt aattatagtc
      961 aagatggtat tattgatacg tatgaagctg tagaaatgtc acgtaaattt ggtattgaag
     1021 tatttaatgt atttttgagt caagatccaa taactgaaga tgttgaacaa acaattcata
     1081 atatttatgg tcaatatgct attttcgttg aaggtgttgc gcatttacct ggacatctct
     1141 ctccattatt aaaaaaatta ctacttaaat ctttataaat aaatttaaaa tatgagttta
     1201 tatgaaattg tgaataaaag tcatcagtta ctatttaaaa agtgga
//
LOCUS       DAKTIP010000365         1241 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.372, whole genome shotgun sequence.
ACCESSION   DAKTIP010000365 DAKTIP010000000
VERSION     DAKTIP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.372"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..1004)
                     /locus_tag="O0305_002864"
     CDS             complement(<1..1004)
                     /locus_tag="O0305_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCY0275947.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     "
BASE COUNT          484 a          223 c          150 g          384 t
ORIGIN      
        1 ttacacaatt catgaacaat aaatgccgat acaaatggta tgacaaaata accaagtgct
       61 aataaaataa tattcattgt tggatcacct gacattcggt taaatgcatt tattgggcca
      121 actaatcctg tatatccaaa tccagcagac aatggtgttc ctttaacctg aagaacgtaa
      181 gcaatgattc cagtaattat tccatttata gtcaatggta tcgaaataat taaatttttc
      241 aagtaaactg ggatcatcat ttttgcagct cctatgagta aaactgcatt tacaccaata
      301 gaattgacac gcaatgagcc aaataagaaa gtaacacagg cagccactat acctaggttt
      361 gctgctccac ttcctagtcc gtttaaacta atcgcagttg caatcgctac taacgatatt
      421 ggtgttacca ttaataatga aaatgccaca ctaataagta tagacattaa caacggattt
      481 aagtctgtaa aagaatgaat tacatttcca attgcttgag taatttttcg aatgtaaggt
      541 aatgtgatta gaccgatacc cccactaacg ataggtacta aaactggtaa tataattaat
      601 tcaaaagatc caagtttgtt ttgtaatacc atatatataa gacatgcaat aataacaacc
      661 aaactcgtat ttataatgtc acctatacct tttaacataa aactattatt gctatataca
      721 acagcacctg aaccaatcat agctgatgta cctactatag cagcacctgc accattaaat
      781 ttaaattgat gagcagctaa aaccccaata ataaatgcca taaatgattg aattagtatc
      841 actaactgat acgttaattc taaaatttca ttaccacttt taaatatttt taatacttca
      901 cctaataaag cattcggaac aagtgcaata acaacaccag caccaataga atttaaaatc
      961 ttactgaaaa actgtttatt atcagcatta tttgcgttac tcataaacga cctccaattt
     1021 gaacattaaa ctcatcatat tatagaaaat taactttaac aatgattagt tggctaattt
     1081 aatctaaata taacaatcca caaattgaat aaagcaaatt taaacaatct actcactaat
     1141 caatgtcatg ttaattttaa aatactacta attacatcct gtgactaata atgtaataaa
     1201 ttgtaaatat taaaatagcc atattactat taagatagat a
//
LOCUS       DAKTIP010000366         1217 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.373, whole genome shotgun sequence.
ACCESSION   DAKTIP010000366 DAKTIP010000000
VERSION     DAKTIP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.373"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            6..1151
                     /locus_tag="O0305_002865"
     CDS             6..1151
                     /locus_tag="O0305_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCY0275948.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGM
                     CTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHI
                     KEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDGTRTSREKLKEYMEIISKYQVRT
                     VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIE
                     TTFLRIGERAGNAPIEEIITILTKKQIESTEFTLPDVYKTSINISKILDFQISENKPI
                     IGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFK
                     SIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            1135..>1217
                     /locus_tag="O0305_002866"
     CDS             1135..>1217
                     /locus_tag="O0305_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0275949.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPT"
BASE COUNT          509 a          136 c          190 g          382 t
ORIGIN      
        1 gtaatatgat tcgaattcaa gataatacaa taagggatgg tatgcaacaa agtaatgttc
       61 gaaaaagtct aattataaaa aaagaagtat tgaaacaaat taacaagtta aatataaatt
      121 ctgttgaagt aggcatgtgt acaactatcg aggatgaatt taatattcat caattcagag
      181 acattttaag tcctgaaaaa gaattagtag tattgactag gcttaatgaa aaagaaataa
      241 aaaaaatagt caaattgaaa attcataatt tagtggtaaa aatactattg ccaatatctg
      301 acttgcatat aaaagaaaag cttaattttt caaataaata ttatattcag aaaatcaaag
      361 actgcttgga tatattaaag aaagataaaa aaggagtaga tatttgtttt gaagatggaa
      421 caaggacttc tagagaaaaa ttgaaagaat acatggaaat tatttcaaaa tatcaagtta
      481 gaacagttac atttgcggac actgtaggat gttcgacacc attagaatac ggagatattt
      541 ttaattactt tgtaaaaaaa tattctaaca taattttttc tgctcattgt cataacgatc
      601 tagggttggc tactgcaaat acattagctg caattttaaa tggtgcaaag caaatagaaa
      661 ctacattttt gagaattggt gagagagcgg gtaatgctcc tattgaggaa ataattacta
      721 ttttgacaaa aaaacaaata gaaagtacgg aattcacttt acccgacgta tataaaacta
      781 gtattaatat ttctaaaatt ctcgattttc aaatatcaga aaacaaacct ataattggtg
      841 aaaatatatt taaacatgaa tcaggaattc atcaagatgg tactaaaaaa aatataaata
      901 tgtatcaata tttagttcct agtgatttag gattgaaaaa ttcacaagtt gttcagtttc
      961 caataagtaa tatttctagt aagaaaatct tgcacaataa atttaaatca atagttaaca
     1021 ctgaagaaat tgatgaaaat atttctttct ataaacttgt gaacaagttt tcacctgaag
     1081 tagcacctga agatacagtg gatttacttc agataataaa aaggaggagt aaagatggaa
     1141 attttaaatg attctgtaaa aagttttgaa aaactttatt tacaattaca agatggtttt
     1201 ccagtgattg ttcctac
//
LOCUS       DAKTIP010000367         1209 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.374, whole genome shotgun sequence.
ACCESSION   DAKTIP010000367 DAKTIP010000000
VERSION     DAKTIP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.374"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..709)
                     /locus_tag="O0305_002867"
     CDS             complement(<1..709)
                     /locus_tag="O0305_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCY0275950.1"
                     /translation="MKRASYSVETKYKAVEMKAAGFPTKEIMKELNIRNRTQVKNWWR
                     WYRNGESYRFSQHVGKQYTYGYRKITALINQCYTSPINHKRVQRVMQKHHLNCRVRPK
                     KTTRIGKPYYKTDNLLQRQFKASCPMEVLTTDITYLPFGHSMLYLSSIMDIYNGEIVA
                     YKIDDKQDQRLVNDTLNQIDIPEGCILHSDQGSVYTSYAYYQLCEEKGIIRSMSRKGT
                     PADNAPIESFHSSLKSET"
BASE COUNT          388 a          202 c          173 g          446 t
ORIGIN      
        1 aagtttcaga ctttagcgag gaatggaaac tttctatcgg ggcgttatcg gcaggtgttc
       61 cctttcggga catacttctg ataatgcctt tttcttcgca taattgataa taagcataag
      121 atgtataaac gctgccttga tcactatgta atatacaccc ctcaggtata tcgatttgat
      181 ttaatgtatc attaactaaa cgttggtctt gtttatcatc tattttatac gccacaattt
      241 ctccgttata aatgtccatt atcgaagata aatacaacat agaatgacca aatggtaaat
      301 aagtaatatc ggttgttaat acttccatgg gacaactcgc tttaaattgt ctttgtaata
      361 aattgtccgt tttataatac ggtttaccta ttcttgtcgt ctttttaggt ctaactcggc
      421 agttcaaatg atgcttctgc atcactctct gtactctctt atgattaatt ggtgatgtat
      481 aacattgatt aatcaatgct gtaatcttac gataaccgta ggtatattgt ttaccaacat
      541 gttgtgaaaa tctataactt tccccatttc gataccatcg ccaccaattt ttcacttgtg
      601 ttctatttct aatatttaat tctttcataa tttcttttgt tggaaatcct gctgctttca
      661 tttcaactgc tttatacttt gtttctactg aataagaagc tcttttcata gaaaaaaaac
      721 acctccgtat gattcatttt aatatgaatt caacgaaagt gtttttatat aattcccact
      781 attggggtca gtctaaaatt aaaattgata acttttttaa taaaatagat acactaattt
      841 taaaacaagg taaaaaaata gaattattaa aacaacgcaa acaaggctta ctacaaaaaa
      901 tgtttgtgta attctgataa cgtcctatta tgttaattga aaatagacaa aaataaattg
      961 agagcatccc ttaccgcaaa gtgaaggatg ctctattttt gttgaaatta atataagctt
     1021 cattctgata gatagtcatt aataattgtt tgcaaatcgt tattaagtac cgttgttaca
     1081 gcgtcatcat tcataaaatc ctcgtagatt ttatcaagaa tttattcctc ttacagacat
     1141 tcgcgagaag tccgttttaa ttttattaga agtaattcag gtttgaacct acctaaatga
     1201 atatatgag
//
LOCUS       DAKTIP010000368         1205 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.375, whole genome shotgun sequence.
ACCESSION   DAKTIP010000368 DAKTIP010000000
VERSION     DAKTIP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.375"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(67..1119)
                     /gene="isdA"
                     /locus_tag="O0305_002868"
     CDS             complement(67..1119)
                     /gene="isdA"
                     /locus_tag="O0305_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCY0275951.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     AKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT          307 a          154 c          236 g          508 t
ORIGIN      
        1 atctatgaca taggttaaat aaaatttaaa ataaaaaata tattaagtta tgagtgtgac
       61 gatgatttat ttagattctt ttcttttgaa aagtaataaa gataatgaac ctaaaagggc
      121 aagtgttgcg aaggcaactg tgctaataaa gttatcaact gaagttaaac cagtttttgg
      181 taattcttta gctttagatg cttgtttagg cgtttcgtta tgttttgtaa ctttgttagt
      241 ttgttgtgat ttattatcac ttacagcttg attgttgctt tcagatttcg ctgttgcaac
      301 atctttaaca ggtgtttgaa ctttattttg ttcttgagca gtttgtgctg ttttaactgt
      361 atgagcagtt tgtgttttag tttgatcttt tgttgtgtca gtacttacaa ctttagtaga
      421 gtgattgtct tcaactttgc ttgttgtagt tacagtaggt ttaacttttt caactttagg
      481 ttgaactggt ttagtttgct cagtaggtgt tttaggttga gctggttttg gttgaaccgg
      541 tttaacattg tttggttttg ctgcgtcagc taatgtagga attgcttttt caaattccaa
      601 atgcgtagta tatctatgat tgtaattaat ttgtggcacg acaatatgta ctttagtagt
      661 taagctctta tatccaggtt caactgcaac attgattgtt ctagtatccg cttttttatt
      721 atcgttaaca acagttgttg ctaattcttg attgtttgca ttgtaaaatt tgtattcttt
      781 ccagaatgat gcattgttta acacggtttg gaaataatat ttattatttt gtttaattac
      841 tttaccaggg tgttgcatat agtcatccat gtgtgacttc tctgaagagc catctttttg
      901 cacttggaaa ttaattggtt gtgatgttgc ttgagaaact tgtgtgcttt gattattagt
      961 tgcgttcgta gcttctgttg ccgcattgac ttgttggctg tctgcgccta tgtatacaag
     1021 ggaacctaaa atgatagatg ctgtacccat tgtaatcttt ttcatagctg atgaacgttg
     1081 ttctgattga tacttactgt ttaaataatg ttttgtcatg ttgttttcct cctaaggata
     1141 caaattatgt gttgttatat gagatgatta accattgcaa ttgataatgg ttatcaatta
     1201 tacta
//
LOCUS       DAKTIP010000369         1174 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.376, whole genome shotgun sequence.
ACCESSION   DAKTIP010000369 DAKTIP010000000
VERSION     DAKTIP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.376"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            186..452
                     /locus_tag="O0305_002869"
     CDS             186..452
                     /locus_tag="O0305_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275952.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            596..904
                     /locus_tag="O0305_002870"
     CDS             596..904
                     /locus_tag="O0305_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275953.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISFINQTAIADKHK
                     NQIAYIFTLCISLSAVFLMSSILSFEYDFIALAFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(1012..>1174)
                     /locus_tag="O0305_002871"
     CDS             complement(1012..>1174)
                     /locus_tag="O0305_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275954.1"
                     /translation="KISLAYNLAIIIGSLIILCILISRGYDIYVILIPILTILASLIN
                     LFCDIKKHK"
BASE COUNT          435 a          174 c          141 g          424 t
ORIGIN      
        1 ttgtaataat ttttcaaaag tatattagaa aattaatttt catgtatttt tcatattaag
       61 cggtaataat ataataatta ataccgcgac ctatttaatt gttttcgcga tgaaaaaggt
      121 ttagaattac aaaaataact attttaaatt tagttacaaa tatattttag aaaggatgtg
      181 aaataatgaa aggctctaaa caaatacttt tgattatggg cattatatct cttattgttt
      241 tatttatttt tacactattc atcatggcgc aatatgcaaa acattatgaa caaaaatccg
      301 acagttccaa cgcacatact ttaaattcat catcagccat aattgagcaa catactatgt
      361 cgaatttagc gtctctagat ttatacgctc ctgttcgtaa cattacttct agtcgtgata
      421 ttcatcccat ttatttcatg acaaaagatt aaaacttcct ttttcataac cccattcatt
      481 caactaataa attaaaaaat gataaaaacg tactgttttt tagaaacata catatataag
      541 aaacgcatcc attactaaaa gacagtacat tatattctat caggaaggtg caacaatgac
      601 cgtcaacatc gtatttagta tcgtattctg cataagtatg gttatattag gcatttatgt
      661 cgcaataact aaagatttca cactaatctc ttttataaat caaacggcca ttgcagataa
      721 acacaagaac caaattgcat atatatttac gctatgtata agtttgagtg cagtattttt
      781 aatgtcttca attctaagct ttgaatatga ttttattgca ttagcattct tatttttaac
      841 gatagcatta ttacttatcg cattattcta cgtttgcttt tataaaatta caaaataccc
      901 ataaatgttt gtgatagtgt gttggttaga tttattgatg cgctgtcagt aagtaattgt
      961 ttgcgaaaaa aatacaacct tgtacgaata caaagttgca ttaatataga atcatttatg
     1021 ctttttaata tcacaaaaca aattgattaa actagctaaa atagtcaaaa ttggaatcaa
     1081 gattacataa atatcataac ctctagatat tagtatacat aatataatta gcgaccctat
     1141 tataattgct aaattataag ccaaactaat ttta
//
LOCUS       DAKTIP010000370         1171 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.377, whole genome shotgun sequence.
ACCESSION   DAKTIP010000370 DAKTIP010000000
VERSION     DAKTIP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.377"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            58..1171
                     /locus_tag="O0305_002872"
                     /pseudo
     CDS             58..1171
                     /locus_tag="O0305_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          469 a          164 c          180 g          358 t
ORIGIN      
        1 ttgggtccct tcgtataatt taataaatac cactaaacta aattaacgag gtgccttatg
       61 tataaaattt ataacatgac ccaacttaca ctaccaatag aaacctctgt tagaattcct
      121 caaaatgata tttcgcgata tgttaatgaa attgttgaaa cgatacctga tagcgaattc
      181 gatgaattca gacatcatcg tggcgcaaca tcctatcatc caaaaatgat gttaaaaatc
      241 atcttatatg catatactca atctgtattt tctggtcgaa gaatagagaa attacttcat
      301 gacagtattc gaatgatgtg gttagctcaa aatcaaacac cttcttataa aactattaat
      361 cgttttagag tgaatcctaa tactgatgcg ttaattgaat ctttatttat tcagttccat
      421 agtcaatgtt taaagcaaaa tcttattgat aataattcaa tttttattga tggtacaaaa
      481 gtagaagcta atgccaatag atatacattt gtgtggaaga aaagtattca aaatcacgaa
      541 tcgaaattga acgaaaattc aaaagcatta tatcgtgact tagttgaaga aaaaataata
      601 ccagagataa aagaagatgg agatagcgat ttaacaatag aagaaataga tttaattggt
      661 agtcatttag ataaagaaat cgaagattta aatcattcta ttgagaacga agattgtgct
      721 caaattagaa aacagacccg taaaaaaaga actgagatta agaagttcaa aaagaaattt
      781 gatgattatt ccgaaagaaa aaataaatat gaagaacaaa aatcgattct taaagataga
      841 aatagttttt ctaaaactga tcatgatgca acttttatga gaatgaagga agaccatatg
      901 aaaaatggcc aacttaagcc aggatacaat ttacaaatag cgacaaattc tcaatttgtt
      961 ttatcctatg acctatttca aaacccgaca gatactagaa ctttaattcc atttttaaca
     1021 atgattcaaa ataccttcgg ttatttgtag ctgatgcagg ttatggtagt gagcaaaact
     1081 atatggccat tatagatgat tttaataaaa cgccacttat tacgtatggc atgtttatta
     1141 aagataaaac tagaaagttt aaaagtgaca t
//
LOCUS       DAKTIP010000371         1168 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.378, whole genome shotgun sequence.
ACCESSION   DAKTIP010000371 DAKTIP010000000
VERSION     DAKTIP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.378"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..383)
                     /gene="sarU"
                     /locus_tag="O0305_002873"
     CDS             complement(<1..383)
                     /gene="sarU"
                     /locus_tag="O0305_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCY0275955.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKY"
     gene            712..1068
                     /gene="sarT"
                     /locus_tag="O0305_002874"
     CDS             712..1068
                     /gene="sarT"
                     /locus_tag="O0305_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCY0275956.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKDLSKKGYLNKCRDPHDSRNVIIVVSVKQ
                     HNYIKNLLSEININET"
BASE COUNT          446 a          128 c          150 g          444 t
ORIGIN      
        1 tgatatttta tgtagtctct ttccttatta aatcctttaa ttattttatc taactcatta
       61 ataacacaag caatcttttt acgttgtatt ggagtaactg aaacaaaaat acggcgttca
      121 tctgcttcgt ttctagactt atttataaat ccttttttag atagtgactt aatgttcttt
      181 acaacatcag attgtttgta aagtatgtca ccaatgattt cttttagtga aatactgtgt
      241 tcatttttgt aataaaaata tgctaatatc aacaattctt ttaatgataa tttatattgt
      301 tgctttaact cttgagtgag aaaaaatatg tacgcttcta catttatgaa tttattgacc
      361 ttttcgaaag tttgatagtc caccttacat tctcctacta ttttctgatg tcccaatact
      421 ttatattatt taaagtaata taaaagcaat ctcttttaaa gacatgttaa cctaaatatt
      481 ttatatataa tatatatata tataaaatat aaataactaa aaatcacaga ataacaaatg
      541 acaaattcat ttattatcca aaaataaaaa caattgaaca aaatcaaaat atagtgttat
      601 gattcatttg tgtttatagc tagcaccaca aactaaatat atttggagtt attactagtt
      661 aaagacaaat attatttgac gtgacaatta aaagtaaggg atgaagactt gatgaatgat
      721 ttgaaaagca agagcaatat taaattaatg aaaagagtat taactacata tgagctgcgt
      781 aaatatttaa aaaaatattt ttgtcttaca ttagataatt atttagtatt agcatattta
      841 gatgtattta aaaatgatga aggtaaatat tttatgagag acattataag ttatatcggt
      901 atagaccaat ctagaattgt taaaagcgta aaagatttat caaaaaaggg ttacttgaat
      961 aaatgtagag acccacatga tagtagaaat gtaatcattg ttgtttctgt aaaacaacac
     1021 aattatatta aaaatctttt gagtgaaata aatattaatg aaacataaag tatgtttcga
     1081 gattttaata tcttggtgca gttaaatgta ttttttaaat gcgttaaggt attttttatt
     1141 ttagaaattc aatgctattg agttgatg
//
LOCUS       DAKTIP010000372         1152 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.379, whole genome shotgun sequence.
ACCESSION   DAKTIP010000372 DAKTIP010000000
VERSION     DAKTIP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.379"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            167..268
                     /locus_tag="O0305_002875"
     CDS             167..268
                     /locus_tag="O0305_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275957.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            284..736
                     /locus_tag="O0305_002876"
                     /pseudo
     CDS             284..736
                     /locus_tag="O0305_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(830..>1152)
                     /locus_tag="O0305_002877"
     CDS             complement(830..>1152)
                     /locus_tag="O0305_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporadically distributed, TIGR04141 family
                     protein"
                     /protein_id="HCY0275958.1"
                     /translation="INALKYPESYNFEELIEAIPDNSENIFNDIQRSTNFSIVWILPI
                     SSIDNMPIKDMVHTSNVINKNFQLTNLGSVLLKNKLVEWSLYLKDQRINPIIYMETPT
                     EDRN"
BASE COUNT          426 a          161 c          175 g          390 t
ORIGIN      
        1 cctaacataa tacaaaaatg gattatcagt attttaatcg tttttgctat agttcaactc
       61 attttataaa actaatcctt ttaactcaga cagcaatttc ccgaaaaata atacctctta
      121 tttaaacaaa tatgtatgca gttcggattt atataaataa aaatttttga tgaaatatcc
      181 atatatgctt ataaatataa atttatcaac tataactaaa aatgataaaa gatttaacgc
      241 cactgatgac cgaattaata ttatttaagt ttttatcaaa cgcatgagta aagtagatgc
      301 acattgggaa ttaatcgaag ctattaaaaa tttacgtgac gaaattgcac caaacacatt
      361 gttaacaatt aacgatgata ttcccgatag aaaaacagga cttgaacttg cagaaaaata
      421 tggtattgat ggcatcatga ttggtagagg cattttccac aatccattca cttttgaaaa
      481 agaaccacgc gaacacacaa gcaaagaact attagatttg ttgagattgc atctatcatt
      541 gtttaacaaa tatgagaaag atgaaatacg acaattcaag agtttgcgta gattctttaa
      601 aatctatgtg cgtggcataa gaggcgctag cgaacttcgc catcaattga tgaacacaca
      661 atcaattgca gaagtacgag cactactaga tgaatttgaa gcccaaatgg acgaagacgt
      721 taaaattgaa ttatagtatg agtaataagg gaaaggagga atttaagttt cctcctttgt
      781 ttgtgtgaaa gtcttttagg tatgaactag ggtgatttaa aataaaagtt taatttctat
      841 cttcagttgg cgtttccata taaataattg gattaattct ttgatctttt aaatagagag
      901 accactctac taatttattt ttaagtaata ctgaacctaa attagttaat tgaaagtttt
      961 tatttattac gttactagta tgaaccatat cttttatggg catattatca atagatgata
     1021 tcggtaaaat ccatactata ctaaaatttg tactccgttg tatatcatta aatatatttt
     1081 ccgagttatc aggaatagct tctattaatt cttcaaaatt atatgattct ggatatttta
     1141 aggcgttaat tg
//
LOCUS       DAKTIP010000373         1135 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.380, whole genome shotgun sequence.
ACCESSION   DAKTIP010000373 DAKTIP010000000
VERSION     DAKTIP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.380"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(423..1067)
                     /locus_tag="O0305_002878"
     CDS             complement(423..1067)
                     /locus_tag="O0305_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCY0275959.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
BASE COUNT          428 a          169 c          134 g          404 t
ORIGIN      
        1 ttttgttagt tatatattca ttttcaattt aaaaataaac acttatttaa atcacattct
       61 acttttgaga tgagctcaaa ctaacattaa attgtttata ttatctatac ttcttcctat
      121 taatgtttcg attttaaaat taatacgtag ctatacttta cttttctaat aaaagtgtaa
      181 ttagaaaggc aagtacacct gtttaaagtt tatcttctaa gatttgttta ctttagaact
      241 atagcagtta atcatttcaa tgattaataa tatctttaca attaaggtaa tatattttta
      301 ttgctttatc agtaaaatct cagtctaagt ttctaactta tgacaatcgc tccagtttaa
      361 ggttgatatt cattattgat tattccaaaa ctttatacaa atttctttat taatttaact
      421 gtttatcttc tgaacatatt agcaaggtaa agtatatcct taataatatt caacaaatta
      481 ataaatagat taaatcccat ttctcttggt gaatagtcac ctctttttaa acgattaaaa
      541 tcatacaaag tataaagtag aaataacaac agactaacga cggtaataat agtgtaaaaa
      601 ataggatttt gaagaaaaat accaattaga ctcgcaataa ttaatgttat taatgtaacg
      661 aataaatatt tgcctatact cgatgcatct ccaacgaaga aataaccaat aataccaaat
      721 gctataaatg caaaaattgc taatgcgata tttttataga aaatatctgg tcctaaattt
      781 tgtaaatacg tggtaaacgt tgcgtatgac aataagccaa tcactacagc gtaaatatgt
      841 gaaataatta aaccgaaccg tctcgctctt tcaaaaacaa gtgttgctaa aataataatc
      901 agtaatccaa atgacaaggg ttgtcgccaa cttaatggta aaaactgacc tagatagcat
      961 cctatgccaa atataatcca ataatacata aaataaagcc acactttact tattgattga
     1021 gcatgctggt tttgatgata atatgaatga tttgtatttt gcgacaaaat aattcctcct
     1081 tctaccgtcg ttttatgcgt caaattttac agtaaatttt gcttcatcaa aagaa
//
LOCUS       DAKTIP010000374         1123 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.381, whole genome shotgun sequence.
ACCESSION   DAKTIP010000374 DAKTIP010000000
VERSION     DAKTIP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.381"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..549)
                     /gene="agrB"
                     /locus_tag="O0305_002879"
     CDS             complement(<1..549)
                     /gene="agrB"
                     /locus_tag="O0305_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCY0275960.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFF"
     gene            785..919
                     /locus_tag="O0305_002880"
     CDS             785..919
                     /locus_tag="O0305_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCY0275961.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
BASE COUNT          435 a          139 c          161 g          388 t
ORIGIN      
        1 aaagaaaata ggtaataatg taatagcttc tattatgatg cctaattgaa tgaattgggc
       61 aaatggctct ttgatgataa gtgtgataat gaaaagggtt aaactaacaa taatcgcata
      121 atattttttt cgtttaataa gtcgcacagg aatgggcttc tttttagttg ctgcaggagc
      181 atatactgag attacaccta aagaaataac tgttaaaata atcataatta aaaagttaat
      241 atgaaaattt actattacta aaggtaaaag tataaatagt ataatacttt ctacataaca
      301 ccaaaaagaa gaaggtgcat gtgcaccatg tgcatgtctt cttattaaat aaaatgttaa
      361 attcgtaatt aacgtaaaca gaaaaatgtt taaaatatag gcaatagtat acataacaat
      421 taatttacct atatttttag ctaagacctg catccctaat cgtacttgca aaaattgaat
      481 atgatctaag ttatttctct tttgaagata cgtggcaaac tggtcaattt tattatcaaa
      541 ataattcaat tttacaccac tctcctcact gtcattatac gatttagtac aatcttttat
      601 cattatattg cctaactgta ggaaataaat acttaactgt taaatgtaat ttgtatttaa
      661 tattttaaca taaaaaaaat tacagttaag aataaaaaac gactagttaa gaaaaattgg
      721 aaaataaatg cttttagcat gttttaatat aactagatca cagagatgtg atggaaaata
      781 gttgatgagt tgtttaattt taagaatttt tatcttaatt aaggaaggag tgatttcaat
      841 ggcacaagat atcatttcaa caatcggtga cttagtaaaa tggattatcg acacagtgaa
      901 caaattcact aaaaaataag atgaataatt aattactttc attgtaaatt tgttatcttc
      961 gtatagtact aaaagtatga gttattaagc catcccaact taataaccat gtaaaattag
     1021 caagtgagta acatttgcta gtagagttag tttccttgga ctcagtgcta tgtatttttc
     1081 ttaattatca ttacagataa ttatttctag catgtaagct atc
//
LOCUS       DAKTIP010000375         1121 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.382, whole genome shotgun sequence.
ACCESSION   DAKTIP010000375 DAKTIP010000000
VERSION     DAKTIP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.382"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            114..914
                     /locus_tag="O0305_002881"
     CDS             114..914
                     /locus_tag="O0305_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCY0275962.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSSHTVQAGESLNI
                     IASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQN
                     LYTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPG
                     PYGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          391 a          196 c          177 g          357 t
ORIGIN      
        1 acttattttt gataatatgg aaatcgtaat ataaaacgaa aacttaattt actatataaa
       61 ttgtcttaat aatttttaaa agtagtaaaa cataatttta aggaggagtc cctttgaaaa
      121 aattagcatt tgcaataaca gcaacatctg gtgcagctgc atttttaacg catcatgatg
      181 cacaagcttc tacacaacat acagtacaat ctggtgaatc attatggagt attgctcaaa
      241 aatacaacac ttcagtagag agtattaaac aaaataacca attagataac aacttggtat
      301 tccctggtca agttatctca gtaggtggaa gtgatgcaca aaatacgtca aacacttctc
      361 cacaagctgg ttcagcatca tcatctcata ctgtacaagc tggtgaatca ttaaatatca
      421 ttgctagcag atatggtgtt tcagttgatc aattaatggc agccaataac ttacgtggtt
      481 atttaattat gcctaaccaa acattacaaa ttcctaatgg tggatcaggt ggtacaacac
      541 caacagctac aacaggtagc aatggcaatg catcatcttt taatcaccaa aatttataca
      601 ctgctggtca atgtacatgg tacgtatttg accgtcgtgc tcaagctggt agtccaatta
      661 gcacatattg gtcagacgct aagtattggg ctggtaacgc agctaatgat ggttaccaag
      721 taaacaacac accatcagtt ggttcaatta tgcaaagcac acctggtcca tatggtcatg
      781 ttgcttatgt tgaacgtgtc aatggtgatg gtagtatctt gatttctgaa atgaattaca
      841 catatggtcc atacaatatg aactaccgta caattccagc ttcagaagtt tctagctatg
      901 cattcatcca ttaattaaat aaattgtact gatatatact agcaattcac atcatgtgag
      961 attgctagtt ttttattttt gaaaaaaatt ttcattttgg tacaaaaaat tatctcaccc
     1021 ttccctatca tacatattta tattttgtat gaatggtagt taggtaaaaa ttaacaacct
     1081 acctatttga ttaaattaaa tgcataatat attaaaaatc a
//
LOCUS       DAKTIP010000376         1101 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.383, whole genome shotgun sequence.
ACCESSION   DAKTIP010000376 DAKTIP010000000
VERSION     DAKTIP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.383"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            63..899
                     /locus_tag="O0305_002882"
     CDS             63..899
                     /locus_tag="O0305_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275963.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
BASE COUNT          362 a          140 c          172 g          427 t
ORIGIN      
        1 ggatattgta ttgccaattt gaaagaagac aaatggattt atattgaatt gtaggtgtaa
       61 agatgctaga taatattatt ttatatttta aaaacttacc gcatactaaa aggtatgtaa
      121 cagaacgttt aaaacaaagt tggaaaagtt ttctaatcgt attagctgca tgtttaatac
      181 ttattattgc aagtgaaacg ctattttcct ttagtcattt gaccgatgtt aaagaggtga
      241 gatggttatt tagaatcatt gtattgattg tttttgcggt agtgatgttc acaatttata
      301 tctcttatca tcattatatg aatgactttt tagttactaa gttatttaat atttccgcag
      361 cgacgccagt agttattatg tctattttaa gttttattat gctagtcatt ttgacgatga
      421 ttagtgcatt ggttaaacca gtaaacttcg aaacatcata tatagcgcta ttctatttta
      481 ttgtgatggc aactattttt gtaggattaa tatccgttac ttttggtctt attcgactgt
      541 taactgaaaa aataaatatc atattttatg gggtttgtgt attgtgtttc ttattgttac
      601 caattatttt cataccaaat ccaaatcatg tatttatcaa tcacatccta atgctaaatc
      661 caatgtatta cattgttaat ggtatagcac aatctatcat atttggtata tcaagtatgg
      721 aaaacattcc atatcatttt tactttattt tattcttatg tttaatagct gcagtaaatt
      781 tcgtattagc aaggtatacg acacacgcaa tttataataa aacatctaaa gtgacacaaa
      841 ctgataatca acaggatgtt tcaaatgata gtacagacga ggcggatact tcttcatgat
      901 gtttagctga cctgttgtgt aaattaatct gtgagaatca gaaacaatta gaatatcatc
      961 tagatattgt tttcctatac tgattttgat atttcgagtc attaaaggta gtttgttagc
     1021 aacaatataa attttagatt ttgtaaaagc attcatagtt ttacattaaa aaaccggaca
     1081 gagtaaaaat cgttttgatt t
//
LOCUS       DAKTIP010000377         1090 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.384, whole genome shotgun sequence.
ACCESSION   DAKTIP010000377 DAKTIP010000000
VERSION     DAKTIP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.384"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(82..1050)
                     /locus_tag="O0305_002883"
     CDS             complement(82..1050)
                     /locus_tag="O0305_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCY0275964.1"
                     /translation="MKPFESHNKQLKILRRRGMEVPSSAKRDLENENYYNIINGYKDL
                     FLELDVNGNFLVPDKYKQGTHFKEVFSLYKLDRKFRNVLLEYLLVFETHIKSRISYYF
                     SEKYREPHSYLYFKNYSSDTSKTDSIVKMVATFSSVMSNRKNKPLKHYINTHNGVPLW
                     ILVNYLTLGNVSKMYSNLDDDLRLEVAKDYKRKLERDYKTRVQITPSDVDSILQQAHM
                     FRNVCAHEERLYDYKIDRAKSRANIFANYNKIYDKEYVPTMNGSYVFDLLISLCLFLN
                     KHDYIKLMKNMDKLISNYSHSFYTITIDDLYTKMNFPDQTKILDML"
BASE COUNT          367 a          179 c          122 g          422 t
ORIGIN      
        1 atccctcact gcaacacagg gcgtttctca gcgtcttaaa ataaaaaaac gccactcgta
       61 agtgacatta aaaaatatct tttataacat atccagtatt tttgtttgat ctggaaaatt
      121 catttttgta tataggtcat ctatagtaat tgtatagaaa gaatgtgaat aattacttat
      181 tagtttatcc atatttttca tcaattttat gtaatcatgt ttattcaaaa atagacatag
      241 tgaaatcaac aaatcgaata cataactacc attcattgta ggaacgtatt ctttatcgta
      301 tattttgtta taattggcga atatattagc tctactttta gccctgtcta ttttataatc
      361 atacaatctt tcttcatgcg cacacacgtt acgaaacata tgtgcttgtt gtagtatact
      421 gtctacatct gatggagtta tttgaacacg tgttttataa tctctttcca atttcctttt
      481 atagtcttta gcaacttcca atcgaagatc atcatccaaa ttggaataca tttttgaaac
      541 attacctaaa gttaaataat tcaccaatat ccatagtggc actccattat gagtattaat
      601 ataatgtttt aatggtttat tttttctatt actcataacc gagctaaatg tagcaaccat
      661 tttcacgatg ctatctgtct tacttgtgtc agatgaataa tttttaaagt ataaatatga
      721 atgtggttct ctatattttt cgctaaaata atatgaaatt cttgatttaa tatgagtttc
      781 aaatactaac aaatactcta ataaaacatt cctaaatttt ctatctagtt tgtataaaga
      841 aaagacttct ttaaaatgag taccttgctt atatttatca ggaaccaaaa aattaccatt
      901 aacatctagt tctaaaaata aatctttata accatttatg atattataat aattttcatt
      961 ttctaaatct cttttagcac tactcggtac ttccattcct cttcttctta gaattttcaa
     1021 ttgtttatta tgactttcaa atggcttcat aaatttaccc ctataaaagt agccataacc
     1081 cgaatagagt
//
LOCUS       DAKTIP010000378         1066 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.385, whole genome shotgun sequence.
ACCESSION   DAKTIP010000378 DAKTIP010000000
VERSION     DAKTIP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.385"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..212)
                     /locus_tag="O0305_002884"
     CDS             complement(<1..212)
                     /locus_tag="O0305_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275965.1"
                     /translation="MTFEEKLSQMYNKIASEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDI"
     gene            complement(224..724)
                     /locus_tag="O0305_002885"
     CDS             complement(224..724)
                     /locus_tag="O0305_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275966.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEIVF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVVFDYVDWMNSEFGPIAKENYYMYKKFGVLPETEYEINKVKEIDQYVKE
                     QDEAEL"
BASE COUNT          341 a          208 c          103 g          414 t
ORIGIN      
        1 aaaatatctt ttgagacatt gcaatcttta ggtatgtctg aataataatt taattcatca
       61 ctacctggtt tagtataatt aaaaatgact tccccagctt gatcagttac ataagctatt
      121 gtaaatactt gctcccattc tactggtatc attccactga tctcacttgc aattttattg
      181 tacatttgac ttagtttctc ttcgaaagtc atgttatctc cccctatagt tcagcttcat
      241 cttgctcttt aacatattga tcgatttctt taactttatt aatttcatat tccgtttctg
      301 gtaaaacacc aaactttttg tacatataat aattttcttt tgctattgga ccaaattctg
      361 aattcatcca atcaacataa tcaaatacaa cattcaattt accatctctt gtaaagtcaa
      421 attcacatga tgtccatggt tctaaatctt cttctttaaa tgcgtttctt aacttcttaa
      481 acaatctata taaatccgtc cacaaatcat aaaatacttt ttcagagaca ttatactctc
      541 tagggatata tgtgtaataa ttcaattcat cgctacctgg tttagtataa ttaaaaacga
      601 tctctccacc tctatcattt acataagcta tcgtaaatac ttggtcccat tctactggta
      661 tcatcccact aatctcattc gcaatcttgt tgtatatttc acttagtttt tcttcgaaag
      721 tcatgttatc gcccctatat tttcaaaata gatattggtt tcatttccgt taaataaaaa
      781 gcactaaaca acacctctac gtcgcttaat gcttcttcaa attaatatta ctcattttaa
      841 acctaaatac actaatggaa ctaaaataac tgcaactatt gttacgataa ctggaattct
      901 aaataatttc gagtctttgc agtattatat tatttgtaaa taatagccct aaaactccta
      961 ctcattattt tgtctttcta tttatttaac tatttataat tatagcaaaa aagatattaa
     1021 tagtatatac ttttttgcca tttcgtaaat aaaatttacc agcacc
//
LOCUS       DAKTIP010000379         1064 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.386, whole genome shotgun sequence.
ACCESSION   DAKTIP010000379 DAKTIP010000000
VERSION     DAKTIP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.386"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            32..250
                     /locus_tag="O0305_002886"
     CDS             32..250
                     /locus_tag="O0305_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCY0275967.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            639..>1064
                     /locus_tag="O0305_002887"
     CDS             639..>1064
                     /locus_tag="O0305_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCY0275968.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYI"
BASE COUNT          363 a          175 c          166 g          360 t
ORIGIN      
        1 atggctcaag tatattaatc gaggtgaggt attgagcttt tacgaattta tgcaaagttt
       61 tcttggtgat gacacaccat taggtgaatt ggttgattgg attaatcaag atagcaattt
      121 ccctagagaa gtgaagagcc atagtgaaat attgtcatat tttcgaacaa atccatgtcc
      181 tgaaagcatc tcagtacctg taattaaacg ggcactatca gttttcaacc aattcacaaa
      241 tgtacaatga tacatgaata catggagtca catcatctca tgttagcgct ctgattgcag
      301 ggcgctattt ttaatgttat tgctacattt agtatattgt gattgctatt ttctttcagt
      361 tatagaacca tgtacctttt ttttaaatca ttttatattt tcccactaat atccgctgtt
      421 aatcaatcct gacatccttg tatcactatg acaattaatt gttaaataca tgaatttcta
      481 cattttatga aaaaatccat ttttattaca attcaacact ttatatgaca acttcattac
      541 agttactttt attgttgatt gcttacattg ttttctaaaa aaaatttgtt atcataatta
      601 acgttgaata aagaaaaaaa ttaacttggg agataaaaat ggaatataaa aagatactaa
      661 ttcgtttatt aattgctttt gcagtacttt tctcagcaga tttcacttat caatccgtcg
      721 aacaaacgca tcaatcgcat gccgcagtta attattatag taaaaaccaa tgtacatggt
      781 gggcatttaa acgtcgcgca caagtcggta aacctgtttc taatagatgg ggcaatgcta
      841 aaaattggta ttacaatgca cgtaaatcaa aatatgcgac tggtcgtaca ccaagaaaat
      901 ttgctgtcat gcaatcaact gcaggatatt atggacatgt cgcagttgtt gaacaagtat
      961 ataaaaacgg tagtattaaa gtttcagaat acaactttta tcgcccatta aaatacaata
     1021 cacgtgtact aagcaaaaag gcagcacgta actttaacta tatt
//
LOCUS       DAKTIP010000380         1060 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.387, whole genome shotgun sequence.
ACCESSION   DAKTIP010000380 DAKTIP010000000
VERSION     DAKTIP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.387"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(171..>1060)
                     /gene="sasG"
                     /locus_tag="O0305_002888"
     CDS             complement(171..>1060)
                     /gene="sasG"
                     /locus_tag="O0305_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCY0275969.1"
                     /translation="KERKFNPDLAPGTEKVTREGQKGEKTITTPTLKNPLTGEIISKG
                     ESKEEITKDPVNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTGKVIE
                     EPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTITTPIT
                     VNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSGPVNP
                     NNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQKGLI
                     FSSIIGIAGLMLLARRRKN"
BASE COUNT          224 a          206 c          169 g          461 t
ORIGIN      
        1 tatgaaaata ttatcaaaaa aatcaaattt ataacaaaaa tacacccctt aaagttaggt
       61 ctttcaatcc aacttttggg gtgtatatca ttctcatcat attctaggtt gtttttaaca
      121 aactaaatat agtgaatgca aatcaactat tatttaaatt atgaattatt ttaattcttt
      181 cttctacgag ccaataacat taatccagca attccaatta tactactaaa gatcaaacct
      241 ttttgcgtgc tttctaaacc tgtttttggt aattctgctc gttttttctc ttgattagct
      301 actgattctt tagcaatttt agatttttta actttatcat ttttatccat tgaatgaact
      361 gggccatttg gttttgctct gtctttcgat aatcctggat tgttaggatt tactgggcca
      421 cttggatgag ttggtctgct cggcttctct gggttttcag gtccttttgg atcttttggt
      481 ttctctccac cgaactctac aatcttatct actggttgtt ttgtgatctc ttctgttggt
      541 tgaccctcgc caactttttc acctgttaat gggttcactg tgattggtgt tgtgattgtc
      601 ttacttcctg gttgtccttc ttgtttcact cgctcttcac caggttgtaa ttttggatta
      661 aactcacgtt ttgtttcaaa cggtatctct actgtttttg tttctggtgt acccgttttt
      721 ggtccgtgtt taatcacatc atccactggc tcttcgatca cttttcctgt gtctggattc
      781 ttgattcctg gtttacctgg tactttttcc gtttgatctg tgggtaagtt tggatcaaag
      841 atatctttat gaccttgcgg tattttctcg ccaccgaatt ctgttaattc attaactgga
      901 tcttttgtga tttcttcttt cgattcacct ttactaataa tttctccagt taatggattt
      961 tttagtgttg gcgtcgttat tgtcttctca cctttttgtc cttctcttgt tactttttct
     1021 gtccctggtg ctaaatcagg attaaattta cgttctttct
//
LOCUS       DAKTIP010000381         1056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.388, whole genome shotgun sequence.
ACCESSION   DAKTIP010000381 DAKTIP010000000
VERSION     DAKTIP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.388"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..326
                     /locus_tag="O0305_002889"
     CDS             <1..326
                     /locus_tag="O0305_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0275970.1"
                     /translation="IPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEFD
                     FTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKEQ
                     DEAEI"
     gene            337..825
                     /locus_tag="O0305_002890"
     CDS             337..825
                     /locus_tag="O0305_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0275971.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAVEKYVKE
                     QE"
BASE COUNT          422 a           99 c          206 g          329 t
ORIGIN      
        1 atatacctaa ggagtataac atctctgtgc aagtatttga tgatttatgg atggatttat
       61 atgatttgtt tgaggaatta agagatttat ttaaagaaga aggacttgaa ccatggacat
      121 catgtgaatt tgactttaca agcgaaggta aattaaaagt ttcatttgat tatatagatt
      181 ggataaatac agagtttgat caattaggcc gtgaaaatta ttatatgtat aaaaaatttg
      241 gggttttacc agaaatggaa tatgaaatgg aagaaattaa agaaatcgat caatatatta
      301 aagagcaaga tgaagctgaa atatagggga gataacatga ctttcgaaga aaagctaagt
      361 caaatgtaca atgaaattgc aaatgaaatc agtggaatga taccagtaga atgggaaaaa
      421 gtatatacaa ttgcctacgt agatgatgaa ggtggagagg ttgtttttaa ttatactaaa
      481 ccaggaagtg aagatttgaa ttattattca gatattccta aagattgcaa tgtctcaaaa
      541 gatattttta agaattcatg gtttaaagtt tatcgaatgt ttgatgagtt aagagaaact
      601 tttaaaaaag aagatttaga accgtggaca tcatgtgaat ttgactttac aagaaaggga
      661 aatttaaaag tatcatttga ttatatagat tggattaaat taggttttgg cccatcagga
      721 aaggaaaact actatatgta caaaaaattt ggtattttac cagatatgga atatgaaatg
      781 gaagaaattc gagcagtaga gaagtatgtt aaagagcaag agtagcagac atgttataaa
      841 agactgtgca aaatcaccct cgttttacat ttgattcaaa gaagaaggta aaagataaga
      901 ttatttgcaa cttaaaaagt caattagctt atcggtatcc atacatcatg gataaatgag
      961 ttcaactaat taacaaatca cgatataaat ttaaaattgt cattaattga aaaggttatt
     1021 gtagtgtatt ttataaagtt gatgtacatc gactta
//
LOCUS       DAKTIP010000382         1056 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.389, whole genome shotgun sequence.
ACCESSION   DAKTIP010000382 DAKTIP010000000
VERSION     DAKTIP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.389"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     misc_feature    <1..122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            160..660
                     /locus_tag="O0305_002891"
     CDS             160..660
                     /locus_tag="O0305_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCY0275972.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            703..>1056
                     /gene="rplL"
                     /locus_tag="O0305_002892"
     CDS             703..>1056
                     /gene="rplL"
                     /locus_tag="O0305_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCY0275973.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGAT"
BASE COUNT          379 a          154 c          221 g          302 t
ORIGIN      
        1 cagtaggagt tatttataac ttaaaattta tcctgccgag gctaaaattg acttgaacgt
       61 gatgatctat gatctttcaa gcactttttg ccgtgggtag aaagtgcttt ttttattaat
      121 tttaaaaaaa gcaccaaaaa tttaaatgga ggtgtctgaa tgtctgctat cattgaagct
      181 aaaaaacaac tagttgatga aattgctgag gtactatcaa attcagtttc aacagtaatc
      241 gttgactacc gtggattaac agtagctgaa gttactgact tacgttcaca attacgtgaa
      301 gctggtgttg agtataaagt atacaaaaac actatggtac gtcgtgcagc tgaaaaagct
      361 ggtatcgaag gcttagatga attcttaaca ggtcctactg ctattgcaac ttcaagtgaa
      421 gatgctgtag ctgcagcgaa agtaatttct ggatttgcta aagatcatga agcattagaa
      481 attaaatcag gcgttatgga aggcaatgtt attacagcag aagaagttaa aactgttggt
      541 tcattacctt cacacgatgg tcttgtatct atgcttttat cagtattaca agctcctgta
      601 cgcaacttcg cttatgcggt taaagctatt ggagaacaaa aagaagaaaa cgctgaataa
      661 tttttagcgt aaaaaaatta aaaataatgg aggaattata aaatggctaa tcatgaacaa
      721 atcattgaag cgattaaaga aatgtcagta ttagaattaa acgacttagt aaaagcaatt
      781 gaagaagaat ttggtgtaac tgcagctgct ccagtagcag tagcaggtgc agctggtggc
      841 gctgacgctg cagcagaaaa aactgaattt gacgttgagt taacttcagc tggttcatct
      901 aaaatcaaag ttgttaaagc tgttaaagaa gcaactggtt taggattaaa agatgctaaa
      961 gaattagtag acggagctcc taaagtaatc aaagaagctt tacctaaaga agaagctgaa
     1021 aaacttaaag aacaattaga agaagttgga gctact
//
LOCUS       DAKTIP010000383         1052 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.390, whole genome shotgun sequence.
ACCESSION   DAKTIP010000383 DAKTIP010000000
VERSION     DAKTIP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.390"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..530
                     /locus_tag="O0305_002893"
     CDS             <1..530
                     /locus_tag="O0305_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275974.1"
                     /translation="YFLPMINWFIPKKYAKISREEFESLNIVKPAKNNTFWPVAGFAV
                     LLTTLTRKYIYLLNIHLEKEIVILTCCMILLGVFALFIYINTKLKLHIFDKNKSNNEK
                     IILIPTFKNICLSLFAYILFGGLSTMALSMLVTSSPQNIIEFLALIGMTACFFLLNMS
                     SVLDKKIHVILKTNK"
     gene            587..703
                     /locus_tag="O0305_002894"
     CDS             587..703
                     /locus_tag="O0305_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275975.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQIKNN"
     gene            726..>1052
                     /locus_tag="O0305_002895"
     CDS             726..>1052
                     /locus_tag="O0305_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275976.1"
                     /translation="MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFI
                     PKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIA
                     ICCITFI"
BASE COUNT          398 a          133 c          149 g          372 t
ORIGIN      
        1 cttatttttt gcccatgatt aattggttta ttcccaaaaa gtacgcgaaa attagtagag
       61 aagaatttga aagtttaaat attgtcaaac ccgctaaaaa taatactttc tggcctgttg
      121 caggatttgc agtgttatta acaaccttaa caagaaaata tatctatttg cttaacatcc
      181 atttagaaaa agaaatagtt atattaacat gctgtatgat acttctaggt gttttcgcat
      241 tgtttatata tataaataca aaattgaagt tacatatttt tgataaaaat aaaagtaata
      301 acgaaaagat catattaata cctacattta aaaatatttg tttatcctta tttgcttata
      361 tattatttgg tggattgtca acaatggctc tgagtatgtt agtaacttca tcccctcaaa
      421 atataataga atttcttgct ttaattggca tgactgcatg cttctttcta ctgaatatgt
      481 catcggttct agataaaaaa attcatgtta ttttaaaaac aaataagtag taaaattgat
      541 taacttaggt agtatcggat acttaaatgt tggttcataa aaagcaatga ttttaaatcg
      601 aggagctatc ttagaacagg gaaataaaac agccaaagtt ataaaaagtg aattaataac
      661 taattatatt atgttagcca cgcttcaaat aaaaaataat tagaataagg tgggattgat
      721 aatcaatgct atgcgaatct aaaatcatca ataaaaaccc caaatataga attattaaat
      781 ataatgatga gtacttaatg atcgatataa taagcacttg gattagttta ttttttcctt
      841 ttattaattg gttcatcccc aaaagatacg tcaaaattag tagagaagag tttgaaaatt
      901 taaatattgt gaaacctgct aaaaagaatg ttttttggcc agttgcaggt atctctactt
      961 tattcgcagt tacattaaga aagtatacac atttacttga cactcaactt gatagaaaat
     1021 tagttattgc tatatgttgt atcacattta ta
//
LOCUS       DAKTIP010000384         1028 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.391, whole genome shotgun sequence.
ACCESSION   DAKTIP010000384 DAKTIP010000000
VERSION     DAKTIP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.391"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..863
                     /locus_tag="O0305_002896"
     CDS             <1..863
                     /locus_tag="O0305_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCY0275977.1"
                     /translation="EHFIMRRSAVEILFATIGLIIGLLISVMVSFILESIGNSIFNHF
                     IPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATPKIIDTSAIIDGRI
                     LEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDILNELYDLDYPTKVI
                     HPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNVNDLSEAIKPNVHQ
                     GDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEVVSLLQTSSGRIVF
                     AKKIEDTVSL"
BASE COUNT          381 a          132 c          174 g          341 t
ORIGIN      
        1 tagaacattt tattatgcgt agaagtgctg ttgaaatact attcgcaaca ataggtttaa
       61 taatcggatt acttatttct gttatggtgt cgtttatatt agaatcaatt ggcaactcta
      121 tttttaatca tttcattcct gtcataatta cgatattact atgttatttc ggtttccaat
      181 ttggccttaa aaaacgagat gaaatgttaa tgtttttacc tgagaatata gcgcgttcca
      241 tgtcacaaca tactaaaagt gctacgccaa aaattatcga cacaagcgca attattgatg
      301 gtcgtatttt agaagtcatt cgttgcggtt ttatcgatgg caatatttta attccacaag
      361 gtgttattaa tgaattacaa attgttgcag attcaaatga cagtgttaaa cgtgaaaagg
      421 gtaaaagagg cttagatatt ttaaatgaat tgtatgattt agactatcct acaaaggtta
      481 tacatccaac taaaacacat agtgatattg atacgatgtt attaaaatta gcaaaacaat
      541 atcatgcaag tattataacg acagatttca acctaaataa agtttgtcat gtacatggta
      601 tcaaagcatt aaatgttaat gatttatcag aagcaatcaa acctaatgta catcaaggtg
      661 atcaactgca tattttactg acaaaaatgg gtaaggagcc tggtcaggca gtaggatatc
      721 tagatgatgg tacgatggta gttgttgata atgctaaaaa tcttattggc agtcatgtca
      781 atttagaagt agtcagctta ttgcaaacat cttcaggaag aattgttttt gctaaaaaaa
      841 tcgaagatac agtatcatta taaaaactgt agttgacgaa aacaaaatag atattaaaag
      901 gtatatgaaa tggcgaagtt tgtaatttga agtataacta taaataaatg caaaacatat
      961 tagttatcaa aaaatacaaa tagtgctaat atatcattga aacaagttca ttaagtgtta
     1021 aattttag
//
LOCUS       DAKTIP010000385         1019 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.392, whole genome shotgun sequence.
ACCESSION   DAKTIP010000385 DAKTIP010000000
VERSION     DAKTIP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.392"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(209..844)
                     /gene="sdpA"
                     /locus_tag="O0305_002897"
     CDS             complement(209..844)
                     /gene="sdpA"
                     /locus_tag="O0305_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCY0275978.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIVVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQAIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
BASE COUNT          402 a          134 c          140 g          343 t
ORIGIN      
        1 tattagagta caaaatatac aagttgtact tatatctagg tatgtgactt ttgattttaa
       61 gcgtaaagta aaagttttta tgtttgtatt agttctaaat tattttgcac ttgatgacac
      121 gtatttttga gtttaagtgg tgactcaagt tcaataaaat aaatatcaga tagataattt
      181 gaaaataata tgaaaggtta tctcaaaatt atctccatat tataaagagc agagctaaca
      241 agttatttaa gaagtgcatg aatattacaa cttctaatcg ttttgttttt atataaatta
      301 ggccaaaaat caaaccagaa tataagtaag gtaaataacc aatccaagtg tcagattcgt
      361 gtaatgatgc aaaaactaaa ctagacacaa ttaaccctaa aaacaagtgt tttctgaaga
      421 taacccttat tatcataccg cgaaaaacaa tttcttccac aatagcgggg atgatggtaa
      481 cagtgaaaat agaaatatag aatggtgtat ttcgtattgc ttgctctaat tgttgttcat
      541 ttgccggtac gtcatgaaaa tataaataaa gattatctaa cccataaatt aatagtgcac
      601 cgcctataat ataaataact tgagacaatg ttaatttttt aaaagagaat aaattgattt
      661 tgaatagcct caatagaatt aaacctacta atacaattag catgctataa agtaaatgta
      721 cttcactgac aactatgccg ataaataaaa tgatagtaat aaaaaatgct gccattaaag
      781 ttaaaaaata atttcctcgt gtaaccgata aatcatcgta aaaaaagtct ttgaattttt
      841 gcatgctaaa tcctttcttt tcatttttta aaattatttt gaatgcaata tgattaaggc
      901 tagattcata atatatgaaa catatttacg attaaaattt atcattgtac ctacctaaaa
      961 agcattagtt tgaaaaatta gcagactgta ttttagcgtc aaatcaccat aaatgaaat
//
LOCUS       DAKTIP010000386         1014 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.393, whole genome shotgun sequence.
ACCESSION   DAKTIP010000386 DAKTIP010000000
VERSION     DAKTIP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.393"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            84..995
                     /locus_tag="O0305_002898"
     CDS             84..995
                     /locus_tag="O0305_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCY0275979.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
BASE COUNT          445 a          107 c          166 g          296 t
ORIGIN      
        1 gtattactaa caggcgttga ctaattcttt taatatttta taattgaaaa tgaatatcaa
       61 ttgaaaaaca aaaggatgat attatgaaaa gattaattgg aattttatta tgtaatttat
      121 ttattttgac tgcgtgttca gcatctgtcg ataaaacaag taactcgaca aagactacag
      181 actataaaat tgaaaatggt gaaacactga aagtaccaga gaaacctaaa agagttgctg
      241 tattaactgg attttatgtt ggtgatttta taaagttagg aatcaaacca attgctgttt
      301 cagatataac taaagattct tcaattttaa aaccttattt aaaaggggtt gattatattg
      361 gagaaaatga tgttgaaaga gttgctaaag caaaaccaga tttaattgtt gtagatgcta
      421 tggataaaaa tataaaaaaa taccaaaaaa tagcgccaac aatcccatat acatacaata
      481 aatacaatca taaagaaata ttaaaagaaa taggcaagtt gactaataat gaagataaag
      541 ctaaaaaatg gattgaagag tgggacgata aaactagaaa agataaaaaa gaaattcaaa
      601 gtaaaattgg tcaagcaaca gcatctgtgt ttgaaccaga tgaaaagcaa atatacatat
      661 ataactctac atggggtcgt ggtttggata ttgttcatga tgcattcggt atgccaatga
      721 caaagcaata taaagataaa ttacaagaag ataaaaaagg ctatgcttcc atttcaaaag
      781 aaaatattag taaatatgct ggtgattata tatttttaag taaaccttcg tacggaaaat
      841 ttgattttga aaaaacacat acatggcaga atattgaagc tgtaaaaaaa ggacatgtaa
      901 tttcatataa agcagaagat tattggttca cagatcctat tacattagaa catttgagaa
      961 gtaaattaaa aaaagaaatt ttaaataaaa aataatagaa ataagttgta aaaa
//
LOCUS       DAKTIP010000387         1009 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.394, whole genome shotgun sequence.
ACCESSION   DAKTIP010000387 DAKTIP010000000
VERSION     DAKTIP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.394"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>1009)
                     /locus_tag="O0305_002899"
     rRNA            complement(<1..>1009)
                     /locus_tag="O0305_002899"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          230 a          298 c          196 g          285 t
ORIGIN      
        1 taaaacgatt ataggtggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
       61 cctcagctta ggacccgact aacccagagc ggacgagcct tcctctggaa accttagtca
      121 atcggtggac gggattctca cccgtctttc gctactcaca ccggcattct cacttctaag
      181 cgctccacat gtccttacga tcatgcttca acgcccttag aacgctctcc taccattgtc
      241 caaaggacaa tccacagctt cggtaatatg tttagccccg gtacattttc ggcgcagtgt
      301 cactcgacta gtgagctatt acgcactctt taaatgatgg ctgcttctaa gccaacatcc
      361 tagttgtctg ggcaacgcca catccttttc cacttaacat atattttggg accttagctg
      421 gtggtctggg ctgtttccct ttcgaacacg gaccttatca cccatgttct gactcccaag
      481 ttaaattaat tggcattcgg agtttgtctg aattcggtaa cccgagaggg gcccctcgtc
      541 caaacagtgc tctacctcca ataatcatca cttgaggcta gccctaaagc tatttcggag
      601 agaaccagct atctccaggt tcgattggaa tttctccgct accctcagtt catccgctca
      661 cttttcaacg taagtcggtt cggtcctcca ttcagtgtta cctgaacttc aacctgacca
      721 agggtagatc acctggtttc gggtctacga ccaaatacta aacgccctat tcagactcgc
      781 tttcgctacg gctccacatt tactgcttaa ccttgcatca aatcgtaact cgccggttca
      841 ttctacaaaa ggcacgccat cacccattaa cgggctctga ctacttgtaa gcacacggtt
      901 tcaggttcta tttcactccc cttccggggt gcttttcacc tttccctcac ggtactggtt
      961 cactatcggt cactagagag tatttagcct taggagatgg tcctcccag
//
LOCUS       DAKTIP010000388         1006 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.395, whole genome shotgun sequence.
ACCESSION   DAKTIP010000388 DAKTIP010000000
VERSION     DAKTIP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.395"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(19..>1006)
                     /gene="brnQ"
                     /locus_tag="O0305_002900"
     CDS             complement(19..>1006)
                     /gene="brnQ"
                     /locus_tag="O0305_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0275980.1"
                     /translation="IFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGY
                     LDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKT
                     NQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMA
                     STGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFII
                     ANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSV
                     ISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
BASE COUNT          363 a          166 c          151 g          326 t
ORIGIN      
        1 cctcttttat attattcgtt attcctgttg atatttaatt ggatcttgtt ttacaaatat
       61 gccgactaga tagcctaata tcgttgcaat aattgctact gggaaccact ctaaagaata
      121 cgctcttaga ggcaatgatt ctataaagtt aattttcagc caacctaact tactaataac
      181 actgaaaatc gacaatataa atacgataat aactggaatt tgttgtgaaa tgcgttttgt
      241 cggtatgaat ttggcaatta aaattaataa tacaacagtt attgctactg ggtatacaat
      301 gcttaatacc ggaattgaca ttgagataac agcatttaaa ccttggttag caataataaa
      361 actcattaaa atgaaaacta atacaaatgc tttgtatgat actttaggta cgattctatg
      421 gaaatattca gaaactgcaa caataagccc gcatgctgta gttagacatg ccagcgccac
      481 aatgatgccc aataaatatt ttccgaatga accaaatcct gttgaagcca ttgtcgttaa
      541 taaatatgtc ccaatgtttc gatctttgga tttcaattga tctaacgtca tgtcacttac
      601 tggcatatga ttaccaatat aacctaatga aatatatatg aaaattaaag ctacggctgc
      661 aattaaacca gcagtcaaag tttgtttgaa tatttgattt gttttagtaa tgcctgttag
      721 ttttactgca ttaacaacaa tcattgaaaa agcaattgct gcaatggcat ccattgttaa
      781 atagccttgt gtaaagcctt cagcaaaact tgaaaaatta gaatgatata gtgcttcatt
      841 gccctttcca gcactattac cgctaaagtc taagtatcct ttaataatca tcgctaaaat
      901 agtaatcaat aataatggtg ttaataatga accaatacga tcgattaact tagatggatt
      961 taaacaaata tacaaaacga ctatgaagta gataatcgta aatata
//
LOCUS       DAKTIP010000389          995 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.396, whole genome shotgun sequence.
ACCESSION   DAKTIP010000389 DAKTIP010000000
VERSION     DAKTIP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.396"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            83..796
                     /locus_tag="O0305_002901"
     CDS             83..796
                     /locus_tag="O0305_002901"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCY0275981.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT          382 a          128 c          192 g          293 t
ORIGIN      
        1 aatcattttt tacaaatggt tgtaagaaaa agacatgcag atgttgttaa aattttaata
       61 agaatcaagg aggctatatt atatggctaa actaaatgta gaagtatttg cggacggtgc
      121 agatattgaa gaaatgaaag cagcttataa aaacaaacaa gtggatggtt ttacaacaaa
      181 tcctagttta atggctaaag cgggcgtaac agattacaaa gcttttgctg aagaagctgt
      241 gaaagaaatt ccagatgctt caatttcatt tgaagtattt gcagacgatt tagaaactat
      301 ggaaaaagaa gcagcaattt taaaacaata tggcgaaaat gtatttgtta aaattcctat
      361 tgtaaataca aaaggtgaat caacgattcc tttaattaaa aaactttcag ctgacaatgt
      421 gagattaaac gttacggctg tttacacaat tgaacaagtt aaagaaataa ctgaagcagt
      481 aactgaaggt gtgccaacat atgtttcagt atttgcagga cgtattgcag atacaggcgt
      541 agatccatta ccattaatga aagaggctgt aaaagttacg catagtaaag acggcgttaa
      601 attattatgg gcaagttgcc gcgaattatt caatgtgatt caagctgatg aaattggtgc
      661 agatattatt acatgcccag cagatgttgt gaaaaaagtg aatacaaact taggtcgcga
      721 tattaacgaa ttatcagtag atacagttaa aggctttgcg aaagatattc aatcttcagg
      781 tctttctatt ctataatata taaatatatt taaaccctca taggcattgt gcttatgggg
      841 gtttttccat catagttatt aatgaagaag aagtcaaaat tcagctaagt aaaagctcga
      901 gttataaaaa agtctaaatt gattcaacag gagtagaaaa ggaaattagt cttgcatatt
      961 ttagaaaatg aataaaaaca gttactatat gcaac
//
LOCUS       DAKTIP010000390          986 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.397, whole genome shotgun sequence.
ACCESSION   DAKTIP010000390 DAKTIP010000000
VERSION     DAKTIP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.397"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            309..863
                     /locus_tag="O0305_002902"
     CDS             309..863
                     /locus_tag="O0305_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCY0275982.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NELWKNVRDALKEANIIDKTDNENVKVTYKLANGGENSIEGTANLNELSNSNNKPINS
                     DSVKSIDLSRKNPNGDRFDANDAWKKLTDKLKEKQIVKDGDTVTIHSKDEKDPKISAK
                     VGEVYNDNKRLMLFKKDIDKITIN"
BASE COUNT          424 a          118 c          147 g          297 t
ORIGIN      
        1 catgtaatta taacataaaa aaatagacaa gtaccgaagt acctgcctaa ataacaacaa
       61 gattaacatg tgaataatgg aaataaaaag tcagcccgaa ggctaactta cgaatagatg
      121 aaaatttgaa cacattgctg tgtctaaaat gattatagca taaataacga atatttccag
      181 ctcaaaatta atatattgta ataataatat tttatatctt tgttaataat tatttaattg
      241 atttacataa ataataattg taaaattact ttgtaatcga ttgcaaataa attataggag
      301 aaaataaaat gaataaaaaa ctattaacaa aaacattgat agcaagtgct ttagttttaa
      361 caacagtagg ttcaggtttt cattcttctt caaattataa tggtattaat aacgttgcaa
      421 aagctgagca aacgacagat aacgaattgt ggaaaaatgt aagagacgct ttaaaagaag
      481 cgaatattat cgataaaaca gataatgaaa atgtcaaagt gacttataaa ttagcaaatg
      541 gcggtgaaaa cagtattgaa ggtaccgcca atttaaatga acttagcaat tctaataaca
      601 aaccgatcaa tagcgatagt gtaaaaagca ttgatttgtc aaggaaaaat cccaatggag
      661 atagatttga cgcaaatgat gcatggaaaa aattaactga taaattaaaa gaaaagcaga
      721 ttgtgaaaga cggtgataca gtaactattc atagtaaaga cgaaaaagac cctaaaatct
      781 cagctaaagt cggtgaagtc tataatgata acaaacgatt gatgttgttt aaaaaggata
      841 tagataaaat cactataaac tagttataga taaagacagg ttactttttt aatgtaactt
      901 gtcttttttt aagaggcagt atctcaaata ttaatttata atttctaaat ctatatctat
      961 actttatcta tttctaaatc tatatc
//
LOCUS       DAKTIP010000391          970 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.398, whole genome shotgun sequence.
ACCESSION   DAKTIP010000391 DAKTIP010000000
VERSION     DAKTIP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.398"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(1..392)
                     /gene="tnpA"
                     /locus_tag="O0305_002903"
                     /pseudo
     CDS             complement(1..392)
                     /gene="tnpA"
                     /locus_tag="O0305_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(696..950)
                     /locus_tag="O0305_002904"
     CDS             complement(696..950)
                     /locus_tag="O0305_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275983.1"
                     /translation="MLIKIRGGNMKRDFFEKWSFILNLILFCAFLFLVVLMFYKDVDL
                     IYIFCTLLISLLPLLNIIKRIGWEINQSSMIYEVILAVID"
BASE COUNT          310 a          184 c          116 g          360 t
ORIGIN      
        1 taattgatta cgaatataat tttcaatcac ttttttattt ctacctactg tatccacata
       61 aaatccttta caccaaaact ttctatttcc atatctatac tttaagttag catgtctatc
      121 aaatatcatt aaattacttt tccttttaaa tagccaacaa atgatgatac cccaagtttg
      181 ggtggtatac taactaacat atggatatga tctttacata cctctgcttc aattatctct
      241 acaccttttc tttcatataa ttgacgtaat ataatcccta tatctttttt tatttttcca
      301 tatatcgctt gtcttctgta tttaggtgca aagacaatat gttacttaca attccatttc
      361 gtatgtgcta aactgtttgt gtcagatgac attaaatagc atctcctcgt gttgattatt
      421 ttggttggct gaccaatatt tactctaaca tgtagagatg catttttttg acaatggtag
      481 aaccttttct ggggagtggg acagaaatga tattttcgca aaatttattt cgccgtccca
      541 ccccaacttg cattgtctgt agaaattggg aatccaattt ctctttgttg gggccccgcc
      601 ccaactcgca ttgcctgtag aatttctttt cgaaattctc tgtgttgggg cccctgacta
      661 gaattgaaaa aagcttatta caagcgcatt ttcgttcagt caattactgc caatataact
      721 tcgtagatca tagaagattg atttatttcc cagcctattc tttttataat atttagcaaa
      781 ggtaataatg atattaataa tgtacaaaaa atataaatca aatcgacatc cttataaaac
      841 atcagaacca ctaaaaacaa aaaagcacaa aataaaatta aatttaaaat aaacgaccac
      901 ttttcaaaaa aatctctttt catatttcca cccctaattt taataagcat tattttatat
      961 tctcttttaa
//
LOCUS       DAKTIP010000392          957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.399, whole genome shotgun sequence.
ACCESSION   DAKTIP010000392 DAKTIP010000000
VERSION     DAKTIP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.399"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            86..586
                     /locus_tag="O0305_002905"
                     /pseudo
     CDS             86..586
                     /locus_tag="O0305_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(646..>957)
                     /locus_tag="O0305_002906"
     CDS             complement(646..>957)
                     /locus_tag="O0305_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCY0275984.1"
                     /translation="YLYPVKFREKTRIGRFFNITNWYEEIHSTDEKYVIATIKNIKNK
                     DLIDYSLYIKKGLVEPFIVFYNQENIFYISNAVIDIISVSKDTLDYIKSEFNDHCASP
                     Y"
BASE COUNT          330 a          152 c          151 g          324 t
ORIGIN      
        1 cctattattt aaaacgtcgt gaagaaaaag gtaaataaat aagccttatg tgctaaactc
       61 aatatgtatt tcctaccaaa gaaaacataa ggagtttgga catcttgaag gagatactat
      121 tgttggtcgt cgtcatggta gtgcaatcat tactttggta gaaagagtat ccaagatgat
      181 tatcacctta cgacccttag gtcgtaaggc aactgatata gaaaacgctc tacactcaat
      241 gttttcatct ttaccttctc atatatttaa atcaattact tttgattgtg gtaaggagtt
      301 ctcaaactgg aaaacattat gtaactgtca cgatatatct atattttttg ctgatccagg
      361 cacgccatca cagcgaggtt taaatgaaca ttctaacgga atacttagac gcagtggatt
      421 agataaagaa ctagatttca atttagtaac agacgatcat atcatcagtg ttgctcaaaa
      481 aatcaatcat cgtcctagaa aatctttagg ttatagaaca ccattggaag tatttatgag
      541 tttcatcgaa gatgataaac ttgtccagct taatttgaca atctaaagat ttataaaaaa
      601 gccgttggtc tcttcgaaac tacggctttt ctctgtgcta taaaatcagt atggcgaagc
      661 acagtgatca ttgaattctg actttatata atcaagagta tctttggata cactgatgat
      721 atctataaca gcattgctaa tgtagaatat attttcttga ttatagaata caataaatgg
      781 ctccactaaa ccttttttaa tatataaaga ataatcaatt aagtctttgt tttttatatt
      841 tttaatagtt gcaataacat acttctcatc ggtagaatgg atttcttcgt accagttagt
      901 aatattaaaa aaacgaccaa ttcttgtttt ttctctaaat ttaacaggat ataagta
//
LOCUS       DAKTIP010000393          957 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.400, whole genome shotgun sequence.
ACCESSION   DAKTIP010000393 DAKTIP010000000
VERSION     DAKTIP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.400"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            123..614
                     /locus_tag="O0305_002907"
     CDS             123..614
                     /locus_tag="O0305_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCY0275985.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVMTNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKNIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            614..766
                     /locus_tag="O0305_002908"
                     /pseudo
     CDS             614..766
                     /locus_tag="O0305_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
BASE COUNT          367 a          110 c          187 g          293 t
ORIGIN      
        1 gggtaccggc gggggccctt cgcttgcaac gcggataaac ttttatgaaa gggggataac
       61 ttgatttatc cctttttcag atacttataa atattaaatt cgatatgtaa aggtgtggtt
      121 atttggtatc tgactttgaa agcaatgata aaatcactga agtagaactt ttaatgcatt
      181 acaatccgaa agtcattaat agcaaaatta aagcgatgag atcacaaata gaaagcttat
      241 atcacttgaa catgaaccat gtgatgacga atgataacga tatgctggta tctgtatcat
      301 atccattaga taaattagta ctttatatta tagaagagaa agataaactt gaatattaca
      361 tgaaaacagc tcaggatagg ctaaatttgt ttaaaaatat catcaaaaac tattctgaaa
      421 atgaacaaca ggatgttatg aggtacatgt taagtagtgg gaaagttaaa aatgaaggag
      481 tcattgaacg tttaaaagta gatatttata aagttgaaag tagaaagcgt caagaacgac
      541 aaaaccagcg agaagaatta catcgaatag agtttaataa gcatttagaa caagttaaaa
      601 aagaactttc atagataagc atgatattaa cgggaatatt cctatattca ttaatattgg
      661 tgaatgggac ggagatgatg aggaattaga taaggcagtg caagatgtat ctaacgctaa
      721 ccataatcat actgtgattg tggatgatat accgttagag gattgaagaa aatgaagcta
      781 tgctatttat aaattaatac taattagttt gataccatag cttatttact gagaaagtag
      841 acttaaatgt aacaacacca gtgtttattg ataatattgg tgggtttgag gagtagcaat
      901 aaaataaagg aggtaattgt gtaaaatatc tctttttgtt atttcttatt tatttac
//
LOCUS       DAKTIP010000394          925 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.401, whole genome shotgun sequence.
ACCESSION   DAKTIP010000394 DAKTIP010000000
VERSION     DAKTIP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.401"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>925
                     /locus_tag="O0305_002909"
     CDS             <1..>925
                     /locus_tag="O0305_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0275986.1"
                     /translation="LYASVLGATLRIDICEIHIEIANYIYEEIFDKEMVYPHILMRDY
                     ARQTIEYISLSKDISNINLEKIRPPYKSNWYKKEYSNLNIDDYIKSLKNKLDSHLHFS
                     IDKIKNSMTTEYGRGTGAYGDFGRYVFGYAVRNWVKGFKSDQDLSNIALMRIFEMGYD
                     AKLHGEFDMWVNRYDNFNNSIERISKKYQWIAYYEILAKLVDKFPDVQYSGLWDDYIR
                     DIDPTLLLLEIDKESKILVPSPLPSHQSNEWVKNTKVFDETKLFLEIDIDNHRYICLS
                     SKFNFEKREKEIPFEDRDSCYFLAMGYFYNK"
BASE COUNT          365 a           95 c          155 g          310 t
ORIGIN      
        1 gattatatgc ttctgtttta ggggcgacac ttcgaatcga catttgtgaa atacatatag
       61 agattgctaa ttatatttat gaagaaatat ttgataaaga aatggtatat cctcatattt
      121 taatgagaga ttatgctagg caaacaattg aatatattag tttatcaaaa gatatatcta
      181 atataaattt agaaaaaatt aggccacctt ataaaagtaa ttggtataaa aaagaatatt
      241 cgaatttaaa tatagacgat tacataaaat ctttaaaaaa caaattagat agccatttac
      301 atttttctat cgataaaatt aaaaactcaa tgacaacgga atatggaaga ggaactgggg
      361 catatggcga ttttggcaga tatgtatttg gttatgctgt acgaaactgg gtaaagggtt
      421 ttaaaagtga tcaagactta agtaatatag cattaatgag gatttttgaa atgggttatg
      481 atgcaaaatt acatggtgaa tttgatatgt gggttaatcg ttatgataac tttaataatt
      541 caattgaaag aattagcaaa aaatatcaat ggattgctta ctatgaaatt ttagctaaac
      601 ttgtagataa atttcctgat gttcaatatt ctggactatg ggatgattat attagagata
      661 ttgatcctac tttactatta cttgaaattg ataaagaatc aaaaatacta gtaccctcac
      721 cacttccttc gcatcaatcg aatgaatggg taaaaaatac caaagttttt gatgaaacaa
      781 aattattttt agaaatagat atagacaatc atagatatat atgtttaagc tcaaagttta
      841 attttgaaaa gagagagaaa gaaataccat ttgaagatag agactcatgt tatttcttag
      901 cgatgggata tttttataat aaaga
//
LOCUS       DAKTIP010000395          921 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.402, whole genome shotgun sequence.
ACCESSION   DAKTIP010000395 DAKTIP010000000
VERSION     DAKTIP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.402"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>921
                     /locus_tag="O0305_002910"
     CDS             <1..>921
                     /locus_tag="O0305_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCY0275987.1"
                     /translation="KGYIENDILDKMKSKYAKNIYLNFKKIFSNLEQSVNNYLELEEE
                     FEEKKKYLSEIYELIDEINIDPYIFLTEHNECNIYKISENEKLELQTYMSKIEKVLLK
                     YQTYLKETCKECLFYPYLLVQGEAGIGKSHLLAHLSKKLRDENHIIYLFLGQFFTKNE
                     DPWHQILNDLEVTNSVDNFLRSISNKAKETKKRAFIIIDALNEGEGKRLWGNYFQSFI
                     NHIKKYSNIALIFSIRTPFEDVILPKNAIQDNNIVVFQHEGFSKEENYNPIVSFCDFY
                     GLELPKLPILNPEFNNPLFLKLMCEYCVNKF"
BASE COUNT          379 a           99 c          134 g          309 t
ORIGIN      
        1 aaaggttata tcgaaaatga tattttagat aagatgaaat caaaatacgc aaagaatatt
       61 tatttgaatt tcaaaaaaat attctctaat ttagaacaat cagtcaataa ttatttggaa
      121 ctggaagaag agtttgaaga aaagaaaaaa tacttaagtg aaatttatga attgatagac
      181 gaaattaata ttgaccctta tatttttctg acagaacata atgaatgtaa tatatacaaa
      241 ataagtgaga atgaaaaatt agaacttcaa acttatatga gtaaaatcga aaaagtatta
      301 ttaaaatatc aaacgtattt aaaagaaaca tgtaaagaat gcttgtttta tccttatttg
      361 ctagttcaag gtgaagctgg cataggaaaa tcacaccttt tagcgcatct atctaaaaaa
      421 cttagagatg aaaatcatat tatttatctg tttttgggac aattttttac taaaaatgaa
      481 gatccatggc atcaaatact taatgattta gaagttacaa attctgttga taatttttta
      541 aggtcaataa gtaataaggc caaagaaaca aaaaaaagag cttttattat tattgatgcg
      601 cttaatgaag gtgaaggtaa aaggttatgg ggaaattatt ttcaaagctt tatcaaccat
      661 ataaagaagt attctaatat agctttaata ttttctatta gaaccccttt tgaagatgtt
      721 atattaccca aaaacgcaat acaagataac aatattgtag tatttcagca tgaaggtttt
      781 agtaaggaag aaaactataa tccaattgta tctttttgtg atttttatgg attagagcta
      841 cctaagttac ctatattaaa tccagaattc aacaatccat tatttttaaa actaatgtgt
      901 gagtactgtg taaataaatt t
//
LOCUS       DAKTIP010000396          921 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.403, whole genome shotgun sequence.
ACCESSION   DAKTIP010000396 DAKTIP010000000
VERSION     DAKTIP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.403"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            42..>921
                     /locus_tag="O0305_002911"
     CDS             42..>921
                     /locus_tag="O0305_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCY0275988.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSL"
BASE COUNT          278 a          133 c          170 g          340 t
ORIGIN      
        1 atgctacaaa atagagaaaa ataaatgggg cgtttattaa catggataca aaagacttta
       61 aacgtttaga aaaaatgtat tccccgcgat acttacctgg attagatgga ttgagggcat
      121 tcgcagttat aggaatcatt atttatcact tgaatgcaca atggttaagt gggggctttt
      181 taggagtaga tacattcttc gttatttcag gttatttaat aacaagtttg ttgataagtg
      241 agtattatcg gacgcaaaaa atcgatttgc tagagttttg gaagcgacga ttgaaacgac
      301 tcattccggc agtgttattt ttaatctgtg tcgtgcttac gttcacactg atatttaaac
      361 cggaattgat tatacaaatg aaacgagatg ctattgcagc tatattctat gtttcaaact
      421 ggtggtacat ttcacagaat gtagattatt ttaaccaatt tgctattgaa ccactcaaac
      481 atttatggtc tttagccatt gaagaacaat tttacttgct tttcccattg gttatcacgt
      541 tcttattaca tagatttaaa ccgagaaata ttattcaaac gctatttatt gtatcgttga
      601 tttctttagg acttatgata gtgattcatt tcatcactgg agataattca cgtgtgtatt
      661 ttgggacaga tacacgactg caaactttat tgcttggttg tatattagca tttatttggc
      721 ctccgtttgc tttgaaaaaa gatatttcta aaaagattgt cgtatcatta gatattatag
      781 ggatatctgg ttttgcggtt ctaatgactt tgttctttat agttggagac caagatcaat
      841 ggatctataa tggaggattt tacattatat catttgcaac tttattcatt attgcaattg
      901 cggtacatcc ttctagttta t
//
LOCUS       DAKTIP010000397          919 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.404, whole genome shotgun sequence.
ACCESSION   DAKTIP010000397 DAKTIP010000000
VERSION     DAKTIP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.404"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>919
                     /locus_tag="O0305_002912"
     rRNA            <1..>919
                     /locus_tag="O0305_002912"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          247 a          210 c          274 g          188 t
ORIGIN      
        1 cactgccgag aaaagcctct agatagaaaa taggtgcccg taccgcaaac cgacacaggt
       61 agtcaagatg agaattctaa ggtgagcgag cgaactctcg ttaaggaact cggcaaaatg
      121 accccgtaac ttcgggagaa ggggtgctct ttagggttaa cgcccagaag agccgcagtg
      181 aataggccca agcgactgtt tatcaaaaac acaggtctct gctaaaccgt aaggtgatgt
      241 ataggggctg acgcctgccc ggtgctggaa ggttaagagg agtggttagc ttctgcgaag
      301 ctacgaatcg aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa cgatttgggc actgtctcaa
      421 cgagagactc ggtgaaatca tagtacctgt gaagatgcag gttacccgcg acaggacgga
      481 aagaccccgt ggagctttac tgtagcctga tattgaaatt cggcacagct tgtacaggat
      541 aggtaggagc ctttgaaacg tgagcgctag cttacgtgga ggcgctggtg ggatactacc
      601 ctagctgtgt tggctttcta acccgcacca cttatcgtgg tgggagacag tgtcaggcgg
      661 gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgctcaaa ggttccctca
      721 gaatggttgg aaatcattca tagagtgtaa aggcataagg gagcttgact gcgagaccta
      781 caagtcgagc agggtcgaaa gacggactta gtgatccggt ggttccgcat ggaagggcca
      841 tcgctcaacg gataaaagct accccgggga taacaggctt atctccccca agagttcaca
      901 tcgacgggga ggtttggca
//
LOCUS       DAKTIP010000398          910 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.405, whole genome shotgun sequence.
ACCESSION   DAKTIP010000398 DAKTIP010000000
VERSION     DAKTIP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.405"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..260
                     /locus_tag="O0305_002913"
     CDS             <1..260
                     /locus_tag="O0305_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275989.1"
                     /translation="LKIYDDNLDNENRVILVPTFKDGSFIVFTYLLLGGCSILFLIWL
                     MTIKPQNLLVFIMWIIITIFFFLISMGSISNKKVYAKLKKQ"
     gene            386..>910
                     /locus_tag="O0305_002914"
     CDS             386..>910
                     /locus_tag="O0305_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275990.1"
                     /translation="MEILLCEVRVVNKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFIN
                     WLIPKKYVQISREDFDNLNIVKPVKNKALWPAIGSILLFGTMFRDKIYIPDSHLEKNC
                     VIIICSVLLLSILVFYIYLNQKVKLSIYNDRSSNGKIMIFPSFKNLCFVLFSYFFCGG
                     LSIMFLDVLISLSIQ"
BASE COUNT          329 a           99 c          138 g          344 t
ORIGIN      
        1 aactaaagat atatgatgat aatctagata atgaaaatag ggttatatta gtgcctactt
       61 ttaaagatgg aagttttata gttttcacat atctattatt aggaggttgt tctatattat
      121 ttttaatatg gctaatgact ataaaacctc aaaatctact ggtatttatt atgtggatta
      181 ttattacaat ttttttcttt ctaataagca tgggctcaat tagtaataaa aaggtttatg
      241 ccaaacttaa aaagcaatag aaattactaa acttagattg tagttcgtaa gttaagtaat
      301 aaacaggaaa atagtatgtt actaaaatac aaaacactta taaagtatct gaaagtgtat
      361 tagatataaa accaacgaga cggaagtgga gatattgctt tgcgaggtta gagttgtaaa
      421 caaaaatccc agatatagaa ttattaaata taaaaacgac tatctgatga ttgatttagt
      481 aagcacctgg ttagttttgt tctttccgtt tattaattgg ttaataccaa aaaaatatgt
      541 ccaaatcagc agagaagatt ttgataattt aaatattgtc aaaccagtta aaaataaagc
      601 tttatggcca gctataggta gcatactttt atttggcact atgtttagag ataaaatata
      661 tatacctgat tctcatttag aaaaaaattg cgttattatc atttgttcgg ttttattatt
      721 aagtattcta gtgttttata tatatttaaa tcagaaagta aagttatcta tctataatga
      781 tcggagtagc aatggaaaaa ttatgatttt tccatcattc aagaatcttt gctttgtact
      841 attttcttat tttttctgtg gtggattatc aatcatgttc ttagatgttt tgattagttt
      901 atctatccaa
//
LOCUS       DAKTIP010000399          903 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.406, whole genome shotgun sequence.
ACCESSION   DAKTIP010000399 DAKTIP010000000
VERSION     DAKTIP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.406"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..682
                     /gene="sarS"
                     /locus_tag="O0305_002915"
     CDS             <1..682
                     /gene="sarS"
                     /locus_tag="O0305_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCY0275991.1"
                     /translation="KVKPEVDMTIKEFILLTYLFHQQENTLPFKKIVSDLCYKQSDLV
                     QHIKVLVKHSYISKVRSKIDERNTYISISEEQREKIAERVTLFDQIIKQFNLADQSES
                     QMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVEFTILAIITSQNKNIVLLKDLIETI
                     HHKYPQTVRALNNLKKQGYLIKERSTEDERKILIHMDDAQQDHAEQLLAQVNQLLADK
                     DHLHLVFE"
BASE COUNT          356 a          145 c          106 g          296 t
ORIGIN      
        1 aaaagtcaag cctgaagtcg atatgactat aaaagaattt atattactga cttatttatt
       61 tcatcaacaa gaaaacacac ttccatttaa gaagattgtt tcagatttat gttataaaca
      121 atcggattta gtacagcata taaaagtact tgtgaaacat tcatatatta gtaaagttcg
      181 aagtaaaatt gatgagcgta atacttacat ttcaatatct gaagaacaac gagagaaaat
      241 tgcagaacgt gttacattgt ttgatcaaat cattaaacaa tttaaccttg cagatcaaag
      301 tgaatcacag atgataccaa aagatagtaa agaatttcta aacttgatga tgtatacaat
      361 gtatttcaag aatattatca aaaaacatct aacattaagt tttgtagaat tcacaattct
      421 agctattatc acttctcaaa ataaaaacat cgttcttctt aaagatttaa ttgaaacaat
      481 ccaccataaa taccctcaaa ctgttagagc tctcaataat ttaaaaaagc aaggctatct
      541 aataaaagaa cgctcaactg aagatgaaag aaaaatttta attcatatgg atgacgcgca
      601 gcaagaccat gctgaacaat tattagctca agtgaatcaa ttattagcag ataaagatca
      661 tttacatctt gtttttgaat aatatctcta ttacgcaagt gtgctgtatt ctaaagtgca
      721 cttgtgtttt ctatttttta ataaaacctc agcacataat gaacaacttt ctattttcta
      781 tatcacttaa aaccatttcc gaaattaaac ctcagcacat tcaaagcccc actttattct
      841 taaaaatatt ttttaactca tatgtattaa accgctttca ttataaaaaa tatctctata
      901 ttt
//
LOCUS       DAKTIP010000400          901 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.407, whole genome shotgun sequence.
ACCESSION   DAKTIP010000400 DAKTIP010000000
VERSION     DAKTIP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.407"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..901
                     /locus_tag="O0305_002916"
                     /pseudo
     CDS             1..901
                     /locus_tag="O0305_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT          350 a          101 c          153 g          297 t
ORIGIN      
        1 aaacaagaat taatatcaat taatagtaga aaaattgata gagcacttaa ttctttacca
       61 tatgcagcta gagaacaata ttttaatgat ttattaatcg atgaattaca aagtactaat
      121 gaaattgaaa atgtatttag tactaaacaa gagattgcac atgcgttaaa taaccaagca
      181 tcagaatttc ttaagttcag aggcctcgtg gatcaatata aagagataga acttaataaa
      241 aaaattaaag ttgataatgt aagagacatt agagcgattt atgataaatt agtttcaaat
      301 gaaattaacg aacaagataa gttagatgga gagctatttc gtaaaaattt tgtcggtgtg
      361 catgatgggt caacgaataa atatatacat gttgggttac aacctgaaac caaaattgtt
      421 gaatttatag gtgaaatgct aacattttta aaatattttg atgcgcctca gccgttcaaa
      481 atcatggcta gtcattatct gtttgaatat atacatcctt tttatgatgg caatggtaga
      541 gttggaagat ttataattgc caagctcttg agtgattatt atgataatta tactgctttg
      601 acattttcat atgttataaa tagaaataaa tcgaaatatt acaaggcgtt tatgactgct
      661 tcaaatcatt tgaattgtgg tgatttgacg gaatttattg acacaatgct tgagctattg
      721 atagcaggac aagaaagaat acttgatgag ttaataccaa aaatggatgc gactgaaaaa
      781 ttaacgcttt atttaactag tcattataaa cagatagatt atgaattttt gtatctcctt
      841 tcaatggata aattgtttgg aaataaaaga aatagataac attaattgat ttagaaaaca
      901 t
//
LOCUS       DAKTIP010000401          893 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.408, whole genome shotgun sequence.
ACCESSION   DAKTIP010000401 DAKTIP010000000
VERSION     DAKTIP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.408"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..341
                     /locus_tag="O0305_002917"
     CDS             <1..341
                     /locus_tag="O0305_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0275992.1"
                     /translation="YSSLIKELARTGVNWKWQMLVTLLFVIPCHELYMRTVLQKELTH
                     FSIPKWVAILITAICSSSLFIYLDNWWIVTFIFVAQVILSLSYEYTRRIATTSVAQIV
                     AIILLLIFNA"
     gene            574..719
                     /locus_tag="O0305_002918"
                     /pseudo
     CDS             574..719
                     /locus_tag="O0305_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
BASE COUNT          290 a          107 c          150 g          346 t
ORIGIN      
        1 gttatagttc attaattaaa gaattagcaa gaacaggtgt caattggaag tggcaaatgt
       61 tagtaacttt actttttgtc attccatgtc acgagttata tatgagaact gttttacaaa
      121 aagaattaac acatttttca ataccgaaat gggtagctat attaataact gcaatttgtt
      181 ctagttcatt atttatttat ttagataatt ggtggatagt aacatttata tttgttgcac
      241 aagtgatatt atcattaagt tatgagtata ctcgacgtat agcaacaaca tccgttgcac
      301 aaattgtagc tattatattg ttattaattt ttaatgctta aataagcttt tcttagacgt
      361 atgtgttcta agaaaagctt ttttgcttaa gaatagcaat acgaagattc gaggcatgct
      421 tcattatagt ggtaatattt tttaacagca agcggttatt attgagatta ggttttagtc
      481 agtttttatt actaaatatt ttataaaaat ggtataatat ttttgagcaa gttgaattga
      541 tggtggctat ctgagtaaag gggggtggtg cctatggcat tacttaacac ttttgaaagg
      601 aaaagcctat tgtgatatct attgcaaacg cattacattt aatgttaagt ttcggtatgt
      661 ttatcgtcac tttcattggt atagtagtag caataataaa tttaagcaat aaaaaataac
      721 catcatttca aaactttgac atagaatgat ggttaaagaa aatctcattt agtcaccgtc
      781 tttttaacgg gctcattagg cgacatgttc ctgcatgtcg tctttttaca tttttatagt
      841 aacatatctt ttatttaatt gtctattttt attagatgtg taacaaaatt ttg
//
LOCUS       DAKTIP010000402          878 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.409, whole genome shotgun sequence.
ACCESSION   DAKTIP010000402 DAKTIP010000000
VERSION     DAKTIP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.409"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            123..878
                     /locus_tag="O0305_002919"
                     /pseudo
     CDS             123..878
                     /locus_tag="O0305_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          362 a          120 c          130 g          266 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 ataggtatac atttgtgtgg aagaaaagta ttcaaaatca cgaatcgaaa ttgaacgaaa
      601 attcaaaaac attatatcgt gacttagttg aagaaaaaat aataccagag ataaaagaag
      661 atggagatag cgatttaaca atagaagaaa tagatttaat tggtagtcat ttagataaag
      721 aaatcgaagg tttaaatcat tctattgaga acgaagatag tactcaaatt agaaaacaga
      781 cccgtaaaaa agaactaaga ttaagaagtt caaaaataaa tttgatgatt attccgaaag
      841 aaaaaataaa tatgaagaac aaaaatcgat tcttaaag
//
LOCUS       DAKTIP010000403          874 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.410, whole genome shotgun sequence.
ACCESSION   DAKTIP010000403 DAKTIP010000000
VERSION     DAKTIP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.410"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..290
                     /locus_tag="O0305_002920"
     CDS             <1..290
                     /locus_tag="O0305_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease
                     C-terminal domain-containing protein"
                     /protein_id="HCY0275993.1"
                     /translation="ALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQE
                     DGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS"
     gene            312..830
                     /locus_tag="O0305_002921"
     CDS             312..830
                     /locus_tag="O0305_002921"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCY0275994.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
BASE COUNT          335 a          107 c          167 g          265 t
ORIGIN      
        1 aagcattaat aactaaacaa gaaacaaata tccagaaaga gggtatgtta ttgtgtcaac
       61 atttaagtgt gaaaccagat acacttaaat tcatgttgaa agttttctta gacttaaaat
      121 ttgtaacaca agaagatggt ttaattcgaa tcaatcaaca acctgataaa agatcgattg
      181 attccagcaa agtatatcaa ttaagacaac aacgtatgga tgttgaaaag caattattat
      241 atcaagattt ttcagaaata aaaaattgga taaagtcaca attgtcgtga gcaatttagg
      301 aggaaatatt aatggattta aagcaatacg tatcagaagt tcaagattgg ccgaaaccag
      361 gtgttagttt caaggatatt actacaatta tggataatgg tgaagcatat ggctatgcaa
      421 cagataaaat tgtagaatac gcaaaagaca gagatgttga tatcgttgta ggacctgaag
      481 cgcgtggctt tatcattggc tgtcctgtag cttattcaat ggggattggc tttgcacctg
      541 ttagaaaaga agggaaatta cctcgtgaag tcattcgtta tgagtatgac ctagaatatg
      601 gtacaaatgt tttaacaatg cacaaagatg caattaaacc aggtcaacgt gtgttaatta
      661 cagatgattt attagctact ggtggtacga ttgaagcagc aataaaatta gttgaaaaat
      721 taggcggtat cgtagtaggt attgcattta taattgaatt gaaatattta aatggtattg
      781 aaaaaattaa agattacgat gttatgagtt taatctcata cgacgaataa taaataatat
      841 aattttatca aatgaaatcc ttcatcaaat gtat
//
LOCUS       DAKTIP010000404          850 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.411, whole genome shotgun sequence.
ACCESSION   DAKTIP010000404 DAKTIP010000000
VERSION     DAKTIP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.411"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            84..701
                     /locus_tag="O0305_002922"
     CDS             84..701
                     /locus_tag="O0305_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0275995.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVIRTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            776..>850
                     /locus_tag="O0305_002923"
     CDS             776..>850
                     /locus_tag="O0305_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCY0275996.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFM"
BASE COUNT          323 a           94 c          143 g          290 t
ORIGIN      
        1 gatacaaaag ggcacaagtg ttttgagaaa cagaaacgat aatatagctg tgtgaatttg
       61 ttattaatag gaagtgataa ttattgcttt gtgaaaccga aattatcaat aaaaatccga
      121 aatacagagt tattaagtat gatgatgagt atttaatggt cgatgtaata agaacttggc
      181 ttgtatactt ttttccattt attaattggt tcattccaaa aaggtgcgcc aaaattagta
      241 gagaagagta tgaaaaactg aacactgtta agccagttaa aaataaaaat ttttggcctg
      301 ttgtaggagg aacaatccta ctaggtgcta cttccagaaa gtacatacac ttacttaata
      361 ttcaattaga aaaaagatcg gttatattta tatgcttcgt tgtatttcta tgtattttaa
      421 tcttttttgt gcttctgaat cgaaaattaa aattgaaagt tttcgataat aaaaaagaag
      481 aacaaaaaat aattttagta ccaacattaa aaaatgctgt tcttatatta tatggctatt
      541 tgttgattgg aggcatgtca atattagctt taagcatgtt gctgacatta gaaaaccaga
      601 atttaataac ttttatagct tggggtatgg ggttaatgtt gtttttcttg atgaatataa
      661 cactcattgt caataaaaca gtcaaagtta taaaaaggtg aataaataac taattatatt
      721 gtattggccg ttcttcaaat aaaaaataat ttgaataagg tggaagtgat aaacagtgct
      781 ttgcgaatct agagtcatta atcaaaaccc taaatataga attattaaat acaataatga
      841 atatttcatg
//
LOCUS       DAKTIP010000405          849 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.412, whole genome shotgun sequence.
ACCESSION   DAKTIP010000405 DAKTIP010000000
VERSION     DAKTIP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.412"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            57..656
                     /locus_tag="O0305_002924"
     CDS             57..656
                     /locus_tag="O0305_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCY0275997.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT          325 a          128 c          151 g          245 t
ORIGIN      
        1 aacacacccg aaattaatta ttataaaagt atattcataa aaggaggaat atacttatgg
       61 catttaaatt accaaattta ccatatgcat atgatgcatt ggaaccatat atagatcaaa
      121 gaacaatgga gtttcatcac gacaaacatc acaatacgta cgtgacgaaa ttaaacgcaa
      181 cagttgaagg aacagagtta gagcatcaat cactagcgga tatgattgct aacttagaca
      241 aggtaccgga agcgatgagg atgtcagtcc gtaataatgg cggtggtcat tttaaccatt
      301 cattattctg ggaaatacta tcacctaatt ctgaagaaaa aggtggcgta atagatgaca
      361 tcaaagcgca gtggggcact ttagatgaat ttaaaaatga atttgcaaat aaagcaacaa
      421 cattatttgg atcaggttgg acttggttag ttgttaatga tggcaaatta gaaattgtga
      481 caacgccaaa ccaagataat ccattaacag aaggcaaaac accaatctta ctatttgatg
      541 tttgggagca tgcctactat ctgaaatatc aaaataaacg tccagactat atgactgcat
      601 tttggaacat tgttaactgg aaaaaagttg atgaattata ccaagcagca aaataatata
      661 acttaatata attgaggtgg agcatctaca aggtgttcca cttttttgta cttaatatct
      721 ttacttattt gtttttgtgt aatgggatag cacgtaaaag tggaaaaata ataagataaa
      781 tttcaactcg aaatacctta ttattgttga ctataacgat gacctaaata ttatatacat
      841 tagacaaca
//
LOCUS       DAKTIP010000406          841 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.413, whole genome shotgun sequence.
ACCESSION   DAKTIP010000406 DAKTIP010000000
VERSION     DAKTIP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.413"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            23..220
                     /locus_tag="O0305_002925"
     CDS             23..220
                     /locus_tag="O0305_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0275998.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT          346 a           88 c          142 g          265 t
ORIGIN      
        1 aaaatttcgc ctcatctaat ttatgaattg ctatgatgaa atattcaata caatcaaaga
       61 attgatagaa aacaaagaga tatcgagtta tcaaattaat aaagatactg ggataagtta
      121 cggtaatatt aatgctatgc gccgtagaga aagaagaata gaaaatttaa gcttaaagaa
      181 tgcaaagatc ttatatgaat atgcgaaaaa ggtattgtaa tataagcatt gagttcaata
      241 ggcatacaat aactgcccaa actagtgact gagtgggtgc ttttatagta ttaaaagttt
      301 tctaaaagtg aaagaaatca tgtttagtga tttacttatg tttttcaaaa tagtataata
      361 attttatata atatatagag cttcagtata ccttttgagt atatatggag ttctttcttt
      421 ttatacaaga tgtatatgta taaatggaaa tatttaatac attacataaa aacctttagg
      481 tgcacttaga gggatgttaa tcataaaaaa agagattttg aaaaatatag gctattatac
      541 tttagaatta caaattataa atcttgccag tggatatggg agttcaacat gtcacttacc
      601 ccaatttgtg gaatgttaaa taactataaa gaggaaagga gaaaaaatga taagaatggt
      661 taaagaaaaa gaacgaatag caaaattaaa tttcgaatag ctgtgacgag cttaataatt
      721 caagtactta attttatcaa atcgttcttt taaaaaggta aagggcaaaa gacctttacc
      781 acattaaata gggaggtggt atttatgaca agagaattaa ggaaaaaatt aactctctat
      841 c
//
LOCUS       DAKTIP010000407          841 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.414, whole genome shotgun sequence.
ACCESSION   DAKTIP010000407 DAKTIP010000000
VERSION     DAKTIP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.414"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            364..>841
                     /locus_tag="O0305_002926"
     CDS             364..>841
                     /locus_tag="O0305_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0275999.1"
                     /translation="MKLRALEYSDLLFVHELNNEYSIMSYWFEEPYESLTELQHLFDK
                     HLLDESERRFIVEDENQVVGIVELVEINYIHRNCEIQIIIKPEFSGKGYAKFAFEKAI
                     IYAFNILNMHKIYLYVDADNKKAIHIYESQGFKTEGLLKEQFYTKGKYKDAYFMSLL"
BASE COUNT          339 a           82 c          131 g          289 t
ORIGIN      
        1 taaaatcata atttagaagt gatttggttt tcaagtgaat ttaaggtgtg aagattaaaa
       61 aatatattaa agaaatactt gataaaattt atgaagattt tatgaataac gacgctatca
      121 caaccttact taacaatgat ttacagacga ttattaatca atatctatca aaataaaaac
      181 tatatactga taaaaatagg gcatctttca ctgagtaata aatcagagat attagagaat
      241 aataagaaaa tgagaattag tatgtagtga attgttaaaa tagatttatt catagtgaat
      301 aatgacttat tcgttattta tatttataat gcaaatatat aagaaaattt tgggagtggg
      361 attatgaaac taagagcatt agagtatagt gatttacttt ttgttcatga attaaataat
      421 gaatattcta ttatgtctta ttggtttgaa gagccttatg aatccttaac ggaacttcaa
      481 catttatttg ataagcattt attagatgaa tcggaaagac gttttattgt tgaagatgaa
      541 aatcaagtcg taggtattgt tgaattagta gaaattaatt acatccatcg taattgtgaa
      601 atacaaataa ttattaaacc tgagttcagt ggtaaaggtt atgcaaaatt cgcattcgaa
      661 aaagcgataa tttatgcatt caatatttta aatatgcaca agatctatct atatgttgat
      721 gcagataaca aaaaagctat tcatatatac gagtcacaag gatttaaaac agaaggatta
      781 cttaaagagc aattctacac taaaggtaaa tataaagatg cctattttat gtcattattg
      841 a
//
LOCUS       DAKTIP010000408          828 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.415, whole genome shotgun sequence.
ACCESSION   DAKTIP010000408 DAKTIP010000000
VERSION     DAKTIP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.415"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(119..>828)
                     /locus_tag="O0305_002927"
     CDS             complement(119..>828)
                     /locus_tag="O0305_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCY0276000.1"
                     /translation="TDAKKQMSIDLYKHTSLENNKPTVMLYYGPSGVGKTELAKEIAK
                     KYKGNITRIQFSMMQNDESFNYIFGDEHAKVSLSKDLVDRETNVVLIDEFDKVLPIYY
                     NVFYQMFDEGIIEDANYKVDVSNCIFILTSNFNSIEEAIVNIGEPVFSRIDSCIKFLY
                     LTQEEKQKVIENKLNEILSSLNEKEKRIVTAYNLLEKYKETEIDIDNIRYLKKIITND
                     IYTIIFEDELFNTDIID"
BASE COUNT          282 a          117 c           77 g          352 t
ORIGIN      
        1 tttatatatt aattaaattt taaaattatt aaactcttaa cttatactac ttacaacctt
       61 acatagatag tactttatta atagataact tatactaaaa aaatataaag catataaatt
      121 aatctataat atccgtatta aataattcat cttcaaaaat tattgtataa atatcatttg
      181 ttattatttt ctttaaatac ctaatgttat cgatatctat ttctgtctct ttatatttct
      241 ctaacaagtt atatgcggta actattcttt tttctttttc atttagagat gatagtattt
      301 catttaattt attttctatt actttctgct tttcttcctg tgttaaatat aaaaatttaa
      361 tacaggaatc tattctagaa aataccggtt ctccaatatt aactattgct tcttcaatac
      421 tattaaaatt agatgttaga atgaaaatac aatttgaaac gtctacttta taattggcat
      481 cttcaataat accttcatca aacatttgat agaaaacatt gtaatagatt ggtaatacct
      541 tatcaaattc atcaattaaa acaacattag tttcacgatc tactaaatct tttgacaatg
      601 atactttggc atgttcatct ccaaagatat aattaaaaga ttcatcattc tgcatcatag
      661 aaaattgtat tctagtgata ttacctttat atttttttgc tatttctttg gctaattctg
      721 tttttccaac acctgatgga ccatagtaaa gcattacggt cggtttatta ttttctaagg
      781 aagtatgttt atataaatct atgctcattt gttttttagc gtctgttt
//
LOCUS       DAKTIP010000409          803 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.416, whole genome shotgun sequence.
ACCESSION   DAKTIP010000409 DAKTIP010000000
VERSION     DAKTIP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.416"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..193
                     /locus_tag="O0305_002928"
     CDS             <1..193
                     /locus_tag="O0305_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCY0276001.1"
                     /translation="TCVPTLLINGEHDPLIKDKNHFKLEAHFLNVTKKIFEHSGHAPH
                     IEEPEAFMNYYLKFLKSVS"
     gene            complement(444..803)
                     /locus_tag="O0305_002929"
     CDS             complement(444..803)
                     /locus_tag="O0305_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCY0276002.1"
                     /translation="MNDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYISENKEK
                     EYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRKKIESL
                     LSRVNKRITEANNEIEL"
BASE COUNT          273 a          113 c          115 g          302 t
ORIGIN      
        1 tacatgtgta cctactttgt taattaatgg ggaacatgat cctttgatta aagataagaa
       61 tcattttaaa ttggaagcgc attttttaaa tgttacgaaa aaaatcttcg aacattcagg
      121 acatgcaccg catattgaag aaccagaagc atttatgaat tattatttaa aatttttaaa
      181 aagcgtatca taatatgtga tatataaacc tagggcataa agtccttagg caatgtgaaa
      241 aagctgatta ctattcatta tttgatagaa atcagctttt tttgaaatgt atttgatata
      301 tactgctcgt tatgcggcta tcttccttat attaagtgcc attagtgcaa aacctcttaa
      361 caattaggta aaaagagcat aaaaaaagga agtttaatag aatgtatcat ctatcaaact
      421 tcaccaaatt gcgctaaaca aaattatagt tcaatttcgt tgtttgcttc agtgattcgt
      481 ttatttactc gactcaataa tgattcgatt tttttacgtt gttgtgcatt aacaagaatt
      541 aatacagttc tttcatcatg ctcattacgt tttttatcga agtaatcttc ttgagataaa
      601 attttaactg ctttaacaac ttgtggttgt ttgtagttta aatgattaat aatatcttta
      661 agatagtatt ctttctcttt gttttcgctg atgtatgtca atacagcgaa ttcttcaaag
      721 ctaattgaaa attccttttt aattaaactt tttaatttgt cagcataagt gaccattgat
      781 aacaactcaa agcaatcatt gat
//
LOCUS       DAKTIP010000410          798 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.417, whole genome shotgun sequence.
ACCESSION   DAKTIP010000410 DAKTIP010000000
VERSION     DAKTIP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.417"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            272..685
                     /locus_tag="O0305_002930"
     CDS             272..685
                     /locus_tag="O0305_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCY0276003.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
BASE COUNT          321 a           97 c          133 g          247 t
ORIGIN      
        1 atgttacaga attaaaactt tatatgagtt ttccagtggt atgttgaata agacgaatga
       61 ccaaacgcta agttaataat aagaaataag attacactgt agattgagaa atttaaagta
      121 aataaaaaat acattataca ataaatataa aactgaaaaa taaattttat ggcaatttcg
      181 tgtcgcgagg acttatcaag tgacaacctt gtttaaatgt ttttaaatta ggaaaaatga
      241 ctataagtac tattaaagga gcgaataaat tatgccaatc gtatattatg atggaaactg
      301 tatatattgt tataactatg tcatttggtt aattcaacat gacctaccaa gaaattatca
      361 atttgccacg ttaaaaggtg aagttggcca acaatttttt aaacaacatc cagaagcggc
      421 aaataaaaat agtgtgattt tacagaaagg tgatcgtata tattttgaat cgcaggcgat
      481 tattaaacta atcacagcac tacctaatcg aactaaatta ttgggcgtag gtttatggct
      541 tgtacctaaa ccagtccgta atttcggtta tcgaatgttt gctaataata gaaatagaat
      601 gtggaaaaca acatggcacc aaccaaatga ttatgaaaaa tcgttctttt tagatgataa
      661 tgcgaaagta aaacttactg attgataaaa catacttgct aattgataat ggatatatta
      721 gatgatgaat taaaatttag gcatttaaat agcggaacac tttacggtta gattagaata
      781 attataaaaa agagagca
//
LOCUS       DAKTIP010000411          795 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.418, whole genome shotgun sequence.
ACCESSION   DAKTIP010000411 DAKTIP010000000
VERSION     DAKTIP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.418"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..223
                     /locus_tag="O0305_002931"
     CDS             <1..223
                     /locus_tag="O0305_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0276004.1"
                     /translation="LEPWTSCEFDFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKK
                     FGIWPEKEYAINWVEKIKDYVKEQDEAEL"
     gene            234..734
                     /locus_tag="O0305_002932"
     CDS             234..734
                     /locus_tag="O0305_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCY0276005.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPDSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEF
                     DFTRDGKLNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKE
                     QEEAEQ"
BASE COUNT          317 a           79 c          169 g          230 t
ORIGIN      
        1 gcttgaacca tggacatcat gcgaatttga ctttacaaga gatggcaaat tgaatgtatc
       61 ttttgattat attgattggg tgaattcaga atttggacca atgggaagag aacattatta
      121 tatgtataaa aaatttggaa tttggcctga aaaagaatat gccataaatt gggttgaaaa
      181 aataaaagat tatgttaaag agcaagatga agctgaacta taggggagat aatatgactt
      241 tcgaaggaaa actaagtcaa atgtacaacg agattgcgaa tgagattagt gggatgatac
      301 cggtagaatg ggagcaagta tttacaatag cttatgtaac tgatcaagct ggagaagtca
      361 tctttaatta tactaaacca gatagtgatg aattaaatta ttattcagac atacctaaag
      421 attgcaatgt ctcaaaagat atttttaaga attcatggtt taaagtttat cgaatgtttg
      481 atgagttaag agaaactttt aaagaagaag ggcttgaacc atggacatca tgcgaatttg
      541 actttacaag agatggcaaa ttgaatgtat cttttgatta tatagattgg ataaatacag
      601 agtttgatca attgggccgt caaaattatt atatgtacaa aaaatttggg gttataccag
      661 aaatggaata tgaaatggaa gaagttaaag aaatcgaaca atatattaaa gagcaagaag
      721 aagctgaaca atagaggtga taacatgact ttcgaagaga aaataagcaa attatataat
      781 gagattgcga atgag
//
LOCUS       DAKTIP010000412          773 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.419, whole genome shotgun sequence.
ACCESSION   DAKTIP010000412 DAKTIP010000000
VERSION     DAKTIP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.419"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..187
                     /locus_tag="O0305_002933"
     CDS             <1..187
                     /locus_tag="O0305_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCY0276006.1"
                     /translation="KQLRKRYDIPTSKAPKLLLKGSGNLKGSSVGYKDIEFTFVEKKE
                     ENIYFSDSLDYKKSGDV"
     gene            239..>773
                     /locus_tag="O0305_002934"
     CDS             239..>773
                     /locus_tag="O0305_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0276007.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVNSQMAIQNKGEALKIKGMLLKIDRNTRSA
                     KGFYYTNEIKTEKYEVAQDNQKKYPVKMINNKFISTEEVKEENIKKEIENFKFFAQYS
                     NFKDLMNYKDGDISYNPE"
BASE COUNT          340 a           74 c          147 g          212 t
ORIGIN      
        1 aaaacaatta cgcaaaagat atgatatacc gacgagtaaa gctccaaagt tattgttaaa
       61 aggttcaggg aatttaaaag gctcatcagt tggatataaa gatattgaat ttacgtttgt
      121 agagaaaaaa gaagaaaata tatactttag tgatagctta gattataaaa aaagcggaga
      181 tgtataatca tggctcaatc agaatatgaa atcaatcccg gaaaaagaga gtgatgaaat
      241 gataaaacgt gtaaataaat tagtgcttgg tattagtctt ctgtttttag tcattagtat
      301 cactgctggt tgtggcatgg gtaaagaagc ggaaataaag aaaagttttg aaaaaacatt
      361 gagtatgtat ccgattaaaa atctagagga tttatacgat aaagaaggat atcgtgatga
      421 tcaatttgat aaaaatgata agggaacatg gattgtaaat tctcaaatgg caattcaaaa
      481 taaaggagaa gctctaaaaa taaaaggcat gcttttgaag atagatagaa atacaagaag
      541 tgcaaaagga ttttactata ctaatgaaat aaagacggag aaatacgaag tagctcagga
      601 taatcaaaaa aaatatccag ttaaaatgat taataataaa ttcatttcta ctgaggaagt
      661 taaagaagaa aacataaaaa aagaaatcga aaactttaag ttttttgcgc aatatagcaa
      721 ttttaaagat ttaatgaatt ataaagatgg agatatatca tataatccag agg
//
LOCUS       DAKTIP010000413          750 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.420, whole genome shotgun sequence.
ACCESSION   DAKTIP010000413 DAKTIP010000000
VERSION     DAKTIP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.420"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..179
                     /locus_tag="O0305_002935"
     CDS             <1..179
                     /locus_tag="O0305_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276008.1"
                     /translation="NGKGSVTFNKKDAEVIDFSLSEIENEEYFMLYRNKSFSVVRDFI
                     EKQEFPENYKIAWY"
     gene            255..503
                     /locus_tag="O0305_002936"
                     /pseudo
     CDS             255..503
                     /locus_tag="O0305_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          298 a           88 c          130 g          234 t
ORIGIN      
        1 gtaacggtaa aggttcagta acttttaata aaaaagatgc agaggttata gatttctcat
       61 tatctgaaat agaaaatgaa gaatatttta tgttatatcg taataagtct ttttctgtag
      121 taagagactt tatcgagaag caagaatttc ccgaaaatta taaaatagca tggtattaaa
      181 gcatcctttc tacacagata aagagaaagc gatgctattt ttatatgctt ttttcattat
      241 tcgttgaaaa agaagagtca caaaagttaa catatttaaa agaacttggt gaagatggcg
      301 aatataaata tgttgccaaa atagatagta aaacatctaa attatgtcat tcactcaacg
      361 gaaaaatatt taaagttaaa gatatgatac caggtgtgaa tgcgccacct atgcatcctt
      421 ggtgtagaag taccacagtg ccacatgtcg gcaattggcg agacaagttc tttaaagagc
      481 gtgaaggtaa atatcaagta gaaggttgtt ttattgaatc aggtgcactt aataataaaa
      541 gtgatgaata cggtatcaaa aggaacagac atgctcaaat ttattataat tcagttagaa
      601 atcgagataa acagatagag atttctaaaa ttgctaaaaa tactaatata aataaaaatt
      661 taattcaaag agtttatgaa catatatttg aaaataagta tttattagaa agtggattta
      721 aacaatttga tcctgacttt tatatggctc
//
LOCUS       DAKTIP010000414          745 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.421, whole genome shotgun sequence.
ACCESSION   DAKTIP010000414 DAKTIP010000000
VERSION     DAKTIP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.421"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..208
                     /locus_tag="O0305_002937"
     CDS             <1..208
                     /locus_tag="O0305_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276009.1"
                     /translation="NIGQNSGNINHLISKELRDKYEWNPSLLSKIDNYLKPAIEIKEH
                     NFSDNSLNINVTFNALYIIKIGKK"
     gene            354..713
                     /gene="sarX"
                     /locus_tag="O0305_002938"
     CDS             354..713
                     /gene="sarX"
                     /locus_tag="O0305_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCY0276010.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDD
                     ISKFNALFEDVEQFLNI"
BASE COUNT          310 a           74 c          115 g          246 t
ORIGIN      
        1 aaacattgga caaaatagtg gcaacattaa tcatttgatt tctaaagaat taagagataa
       61 atatgaatgg aaccctagtt tactatctaa aattgacaac taccttaaac cagcaataga
      121 gattaaagag cataatttta gtgataattc cttgaatatt aacgttactt ttaatgcgtt
      181 atacataatt aaaataggaa aaaaataaca ccttgatatg tattgcaaaa tttaatttgc
      241 atttgttggt aattttgaca acgtattaaa aacaaatgag aaagacaatg gctgaaaaat
      301 ttaagataat aatgacagaa gcattgtctt tatatatttg ggggtgcaac attttgaata
      361 ctgagaaatt agaaacattg cttggcttct ataaacaata taaagcatta tctgaatata
      421 ttgataaaaa atataagttg tcgctaaatg atttagcagt cttagattta acgatgaagc
      481 attgcaaaga tgaaaaagta cttatgcaat catttttaaa aactgcaatg gatgagctag
      541 atttaagtag gacaaaatta ttagtttcta taagaagact aattgaaaaa gaaagactta
      601 gtaaagttag atcatctaaa gatgagcgta aaatttatat ttatttaaat aatgatgata
      661 tatctaaatt taatgcttta tttgaagatg tagaacaatt tttaaatatt taattgaaat
      721 tgagtgtcga aagcatagaa tttgc
//
LOCUS       DAKTIP010000415          741 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.422, whole genome shotgun sequence.
ACCESSION   DAKTIP010000415 DAKTIP010000000
VERSION     DAKTIP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.422"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            57..407
                     /gene="scn"
                     /locus_tag="O0305_002939"
     CDS             57..407
                     /gene="scn"
                     /locus_tag="O0305_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCY0276011.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
     gene            complement(460..720)
                     /locus_tag="O0305_002940"
     CDS             complement(460..720)
                     /locus_tag="O0305_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276012.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
BASE COUNT          333 a           95 c           99 g          214 t
ORIGIN      
        1 ttttttttag tcagtcatta aaataaatta gtactaatta ctaaggagaa taaaaaatga
       61 aaattagaaa atctatactt gcgggaactt tagcaatcgt tttagcatca ccactagtaa
      121 ctaatctaga taaaaatgag gcacaagcta gcacaagctt gccaacatcg aatgaatatc
      181 aaaacgaaaa gttagctaat gaattaaaat cgttattaga tgaactaaat gttaatgaat
      241 tagctactgg aagtttaaac acttattata agcgaactat aaaaatttca ggtcaaaaag
      301 caatgtatgc tcttaagtca aaagacttta agaaaatgtc agaagcaaaa tatcaacttc
      361 aaaagattta taacgaaatt gacgaagcac taaaaagtaa atattaaaaa aaccaccctt
      421 ttacgggtgg ttttaatttt ctagataata taaaagtgtt cataaataaa acagtatagg
      481 caaacaataa agtattgaaa aaagtaagtt taatatgaaa attgttaaat gaacgacatc
      541 ttttgttttt ataaatatca agaaaataat caaactcaaa ataaataacg taactgtagt
      601 cataggcgtc catacataat cagcattagt cattaagaat ggtgcagcca ttatgaaaaa
      661 atttataatg cagatgaaat agacaattag actataaatt agataaataa caatacacac
      721 ccttcataaa taaataattt a
//
LOCUS       DAKTIP010000416          739 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.423, whole genome shotgun sequence.
ACCESSION   DAKTIP010000416 DAKTIP010000000
VERSION     DAKTIP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.423"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..542)
                     /locus_tag="O0305_002941"
     CDS             complement(<1..542)
                     /locus_tag="O0305_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCY0276013.1"
                     /translation="MKKNIMNKLVLSTALLLLETTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIR"
BASE COUNT          241 a          116 c          116 g          266 t
ORIGIN      
        1 tgacgaatac ggaaatctaa ttcttttaaa gttaatactg gtttatttcc tttgtaaaat
       61 tcatgtccac cgatgatagt ttgttgttta tcactatatt ttaaaaatag tctataaggc
      121 ttatcagatg aagtagctgc tggcgtaaca ccaccagtaa atgtttcatc ataagtccag
      181 taaccttcaa ctgtgtcctc atctttagtt ccaaatatat caacgtattt atttcttaac
      241 tgattaatgt ttccccaact ctcggatcca aacacttgaa tatgactata ccaaacccac
      301 gtttgcaatg ttgcatgaat gctaccattg ggcttttgcc ataaccattt tccagataat
      361 gaaaaatgcg gctgagtgta atatttgatt agttcattga tattagtctc gttttcactg
      421 atattatagg cttttgcttc agatgaaaaa ctgattggtg ttttaggaag ttgtgttgat
      481 gtagtttcta aaagtaacaa tgctgttgat aaaactaatt tattcatgat gttctttttc
      541 atatgaaaat ctcctttgcg tgaattaccc aaagtatata agctattaca ccgattcgga
      601 attaaataaa agctaaaact atgttaaata aacttaaaca gttagtagtg ttatttaagc
      661 aaaacttatc atttttaagt tttggacaga aacagtatct aataaagtag gcgggagtta
      721 tatattaaaa acgatacgt
//
LOCUS       DAKTIP010000417          731 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.424, whole genome shotgun sequence.
ACCESSION   DAKTIP010000417 DAKTIP010000000
VERSION     DAKTIP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.424"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            351..443
                     /locus_tag="O0305_002942"
     CDS             351..443
                     /locus_tag="O0305_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276014.1"
                     /translation="MFIFKTTSKSHFHNNVKSLECIKIPINKNR"
BASE COUNT          271 a          115 c           68 g          277 t
ORIGIN      
        1 tctccaacca atctatttac attagtgtgt aatactaata aatcacgtgc atttacgctc
       61 actagctttc atctcctcat ttttgctttt caaatttatt aagtatgaat aaaaaataca
      121 tccgaacaaa caaaatctct atattctaat tcgatttaca tagattatac actaaaaaaa
      181 tgattctcaa tgcaaatgaa tgtgtctctc atagtctaaa aactcgtatg tttcgaattt
      241 tatgattcat tgataatagt accagttaaa agaactaaga ttgtaattat ccccccttaa
      301 aattcaagaa tatattttct taatctatca gcaagaggtt atatttcaaa gtgtttattt
      361 ttaaaacaac atctaaaagc cattttcata ataatgttaa atcattagaa tgtataaaga
      421 ttccaattaa taaaaataga taaaatgcaa taaagttcca aatacttatt attattcatc
      481 acaaattacg tgatgccctc tacaacacta aatcaaggat gatatttatt ttatatacat
      541 tagcattcat tgtttgctta cccacattat cctgtttagt cttttttata actctagtct
      601 tgaatgtcat atttacaaag aaaaaaacac taaaatattt aaaactaaca ctatttattc
      661 actttgttct tttatagcac cttttatgtt tattttcttg tattcaataa atagtatgga
      721 gatgataatt t
//
LOCUS       DAKTIP010000418          729 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.425, whole genome shotgun sequence.
ACCESSION   DAKTIP010000418 DAKTIP010000000
VERSION     DAKTIP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.425"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>729
                     /locus_tag="O0305_002943"
     CDS             <1..>729
                     /locus_tag="O0305_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCY0276015.1"
                     /translation="RDNWVTNFSNEKALVNAKLLVDNYNSEIDRLIDILDSRERINLV
                     NKDETFISWTRGLTQKFSKGKNISINPERIVKFMHRPFTKKWIVYDKNIMEMPSRYYN
                     IMENTGQVIYIQGQGMNKEFSAMITDILPNFQFIGNGKGFATYKGKDSLRLVDNISNS
                     FKKKINLNSEEIVYYIYAILHHKYYVNKYSSDLSKGFPRIPILKDVYGFVEIGRELVE
                     LHLNYEKQLNWDGVEIIYNNMNPN"
BASE COUNT          300 a           70 c          128 g          231 t
ORIGIN      
        1 aagagataat tgggtaacaa atttttcgaa tgaaaaagca ttagtaaatg caaaattact
       61 tgtagataac tataattctg aaatagatag attaattgat atcttagatt caagagaacg
      121 tataaattta gtgaacaaag atgaaacatt tattagttgg acacgaggac ttacccaaaa
      181 attttctaaa ggaaaaaata tttcaattaa tccagagaga attgttaaat ttatgcatag
      241 accatttaca aaaaaatgga tagtgtacga caaaaatatt atggaaatgc caagtagata
      301 ttataatata atggagaata ctggacaggt gatttatata caaggacagg gaatgaataa
      361 agaattctcg gctatgatca cggacatatt acctaatttc caatttattg gtaatggcaa
      421 aggctttgct acatacaaag gtaaagatag tttgaggctt gtagataata tttctaattc
      481 ttttaaaaag aaaataaatc ttaattcaga agagatagta tattatatat atgcaatttt
      541 gcatcataaa tattatgtga acaaatattc aagtgatttg agtaaaggtt ttccgcgaat
      601 accgatatta aaggatgtat atggatttgt tgaaattggt agggagttag tggaattaca
      661 tttaaattat gaaaaacaac tcaattggga tggtgtagaa ataatttata ataatatgaa
      721 tccgaatta
//
LOCUS       DAKTIP010000419          726 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.426, whole genome shotgun sequence.
ACCESSION   DAKTIP010000419 DAKTIP010000000
VERSION     DAKTIP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.426"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>726)
                     /locus_tag="O0305_002944"
     CDS             complement(<1..>726)
                     /locus_tag="O0305_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCY0276016.1"
                     /translation="FPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQ
                     TEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFS
                     IKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQ
                     KIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIE
                     KYLKERNERSDKGQMLSVTINSGI"
BASE COUNT          207 a          142 c           91 g          286 t
ORIGIN      
        1 aatgccacta tttatagtta ctgaaagcat ttgccctttg tcagaacgtt cgtttctctc
       61 ttttaaatat ttttctattt tgctattttc ccaatctgga taatcttcac cattctcatc
      121 tttgaatcgc agttcctgtg agaaaatttt ctgcatatag ccttttttct gttgttgaag
      181 taattcaagc ttttgttctt ctaattcaat ttgtcggtcg agtttgctga agaacttgcc
      241 tattttttgc tgttcttcta aacttggata tttaattaga atagtaaaaa aatcattcac
      301 agaaacattt aataatccgt gatttcttgc accctcaact gcaattccag aaacttctct
      361 ataccagtgt gtcgaatcaa aatatgcttc catgaagtct ttagacattt cacttttaat
      421 agaaaaacaa atatacaaag aggacaatac accactatca tatctagtta atcttttaat
      481 agcccctaat gggtatccat tagaataact tttgttatac gcgaattctc cattctttat
      541 tagtgtataa ttttctagat ttttcgacga aactgattta ctaaaatatt ctgtttgatc
      601 aattaaacct aactgtccgg atattgttaa aggctttttc gattctaagt ttttattttt
      661 cctaattact ctatctgtaa gatcccctaa ctgcttctct tcccattcgc cttcaaaccc
      721 tgggaa
//
LOCUS       DAKTIP010000420          719 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.428, whole genome shotgun sequence.
ACCESSION   DAKTIP010000420 DAKTIP010000000
VERSION     DAKTIP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.428"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            121..225
                     /locus_tag="O0305_002945"
     CDS             121..225
                     /locus_tag="O0305_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276017.1"
                     /translation="MYFKNSSLNYIPTTFGGEPKKKHFPKMESASSEP"
     gene            complement(216..288)
                     /locus_tag="O0305_002946"
     tRNA            complement(216..288)
                     /locus_tag="O0305_002946"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(253..255),aa:Lys,seq:ttt)
     gene            complement(292..366)
                     /locus_tag="O0305_002947"
     tRNA            complement(292..366)
                     /locus_tag="O0305_002947"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Gln,seq:ttg)
     gene            complement(376..459)
                     /locus_tag="O0305_002948"
     tRNA            complement(376..459)
                     /locus_tag="O0305_002948"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(423..425),aa:Tyr,seq:gta)
     gene            complement(489..564)
                     /locus_tag="O0305_002949"
     tRNA            complement(489..564)
                     /locus_tag="O0305_002949"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(529..531),aa:Val,seq:tac)
     gene            complement(572..643)
                     /locus_tag="O0305_002950"
     tRNA            complement(572..643)
                     /locus_tag="O0305_002950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Glu,seq:ttc)
     gene            complement(645..719)
                     /locus_tag="O0305_002951"
     tRNA            complement(645..719)
                     /locus_tag="O0305_002951"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(685..687),aa:Asn,seq:gtt)
BASE COUNT          223 a          170 c          160 g          166 t
ORIGIN      
        1 agaaatttca acaactttaa agcacgtata atgatgattt tcagcttgta caaaggagaa
       61 aaaaagaaga caaccaagcc caataatgga ctggccgcct aataataaaa gctctaaaag
      121 ttgtatttta aaaatagttc tttaaattat atacccacca catttggtgg agaacctaaa
      181 aaaaagcact tcccaaaaat ggaaagtgca agtagtgagc catagaggat tcgaacctct
      241 gaccctctga ttaaaagtca gatgctctac caactgagct aatggctcta atggctgggc
      301 tagctggatt cgaaccaacg agtgacggag tcaaagtccg ttgccttacc gcttggctat
      361 agcccaatat atagatggtg gaggggggca gattcgaact gccgaacccg aaggagcgga
      421 tttacagtcc gccgcgttta gccacttcgc tacccctcca gcttattcat ataatttaat
      481 aatcaaaatg gtggagaatg acgggttcga accgccgacc ctctgcttgt aaggcagatg
      541 ctctcccagc tgagctaatt ctccaaaata atgactccta cgggactcga acccgtgtta
      601 ccgccgtgaa agggcggtgt cttaaccgct tgaccaagga gccatggctc cacaggtagg
      661 actcgaacct acgaccgatc ggttaacagc cgatagctct accactgagc tactgtgga
//
LOCUS       DAKTIP010000421          715 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.429, whole genome shotgun sequence.
ACCESSION   DAKTIP010000421 DAKTIP010000000
VERSION     DAKTIP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.429"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            347..715
                     /locus_tag="O0305_002952"
                     /pseudo
     CDS             347..715
                     /locus_tag="O0305_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          289 a           71 c          120 g          235 t
ORIGIN      
        1 tatcattatg catattacaa tgtactttgt accatttgaa tctcggaaaa tgccactatt
       61 tatgacgata atttttacaa tctaaaagca gtaaagactc ataatataac caaaaacatt
      121 aataaaagtt tgttaaatga taaggggatg agtattggtg aatttactca aaaggtttaa
      181 gggaaaaatc ctacctggag agattcaaaa actaaatgga ctatttccaa aaataaaggg
      241 caatcacatg gtggttctta ttggaagtta ataaataaca aaggaaaaag aatagcttct
      301 ttaactaaag aaggaaaaat cttaagggaa taaggtgttg taaattatgt gctttgatat
      361 aaataatttg gatataaaaa agttgaattt tagaaaggta aaaaacgcaa ttcatttaag
      421 tttaagtggc gataaatacc agtttttgga ggacggtaaa actatcaata atacttattt
      481 ttagctgtat atgataatgc aataaacatt tttgaagatt tgttttcgcc ttctgattct
      541 ataaatttgg ttcatgtagt atatgtttat agtgaacctt ataagaaaac aaatgttata
      601 aaaaaattta catcgttaac aatagatgaa ttgtattact ttaaagaata taaaaaggag
      661 aaggatgata atattttgtg cgaggagttt tcatataagt gttccataaa agata
//
LOCUS       DAKTIP010000422          709 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.430, whole genome shotgun sequence.
ACCESSION   DAKTIP010000422 DAKTIP010000000
VERSION     DAKTIP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.430"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..125
                     /locus_tag="O0305_002953"
     CDS             <1..125
                     /locus_tag="O0305_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0276018.1"
                     /translation="KSVKKYEEVLKVFDDDEMAVIIEFLNRSIEELKKEDGLND"
     gene            118..>709
                     /locus_tag="O0305_002954"
     CDS             118..>709
                     /locus_tag="O0305_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCY0276019.1"
                     /translation="MINSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAVTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIE"
BASE COUNT          235 a           85 c          128 g          261 t
ORIGIN      
        1 aaaaatcggt aaaaaaatat gaagaggtac ttaaagtctt tgatgatgat gaaatggcag
       61 ttattataga gtttttaaac cgtagtatag aagaattaaa aaaagaagat ggtttaaatg
      121 attaatagta atatcagatt atatattcta ggatttttag catttttcgc atcattgatt
      181 caaaacatat acacgcctat tattccgaga ctatatgacg attttcaagt accactattt
      241 tggataaatg cgacagttgg cggttttata tttatcgttg cagttatgca aattgtactt
      301 ggtagaagta tagattctcg tgattcgaaa aaagtacttt taacgggatt gggaatagta
      361 atcatatcta gctttatttg tgcagtgaca cataacttta tattatttgc tatatcaagg
      421 ttatttcaag caattggttg cggaattatt cctctcgtga ctttaacttt attagccaaa
      481 ctaagtacag ataatggtag agctcaagct atggcgaact atcaaatatt tttatcatgt
      541 gcaccagctt tggcaccaat cttagggagt actttaggtg caagatggga ttatattggt
      601 atttttagtt ttttgctcgt tatatctatc gtattatttt tgattatctt ttttattgat
      661 attccaaatg ttgaaaaagg gattgtaaaa ttaactgaaa aaattgaag
//
LOCUS       DAKTIP010000423          709 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.431, whole genome shotgun sequence.
ACCESSION   DAKTIP010000423 DAKTIP010000000
VERSION     DAKTIP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.431"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>709
                     /gene="fmhC"
                     /locus_tag="O0305_002955"
     CDS             <1..>709
                     /gene="fmhC"
                     /locus_tag="O0305_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCY0276020.1"
                     /translation="SVLDLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETN
                     RFYKLFQMAEEKHGFHFMNEDYFKRMQEIYKDKAMLKIACINLNEYQDKLKIQLLKIE
                     NEMMTVNRALNENPNSKKNKSKLNQLNMQLSSINNRISKTEELIFEDGPVLDLAAALF
                     ICTDDEVYYLSSGSNPKYNQYMGAYHLQWHMIKYAKSHNINRYNFYGITGVFSNEADD
                     FGVQQFKKGFNAHVEELI"
BASE COUNT          301 a           64 c          116 g          228 t
ORIGIN      
        1 ttctgtattg gatttaaaag ataaagatga gaatcaactt ttaaaagaaa tggaatacca
       61 aactagaaga aatataaaaa agactattga gattggtgtt aaggttgaag atttatctat
      121 tgaagaaaca aatcgatttt ataaattgtt tcaaatggct gaagaaaaac atggttttca
      181 tttcatgaat gaagattatt ttaaacgaat gcaagaaata tataaagata aggcaatgtt
      241 aaagatagct tgtataaatc ttaatgaata tcaagataaa ttaaaaatac aattattgaa
      301 aatcgaaaat gaaatgatga ctgtgaacag agcattaaat gaaaatccaa attctaaaaa
      361 aaataaatca aaattaaatc agttaaatat gcaattatct agtattaata atagaattag
      421 taaaaccgaa gaactaatat ttgaagatgg acctgttttg gatttagctg ctgctttatt
      481 tatatgtact gatgatgaag tttattatct atcaagtgga tcaaatccga aatataatca
      541 gtatatgggt gcatatcatc tacaatggca tatgataaaa tatgcaaaat cacataatat
      601 taataggtat aatttttatg gaataacagg cgtctttagt aatgaggcgg atgattttgg
      661 tgttcaacaa tttaaaaagg gttttaatgc acatgttgaa gaattaatt
//
LOCUS       DAKTIP010000424          700 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.432, whole genome shotgun sequence.
ACCESSION   DAKTIP010000424 DAKTIP010000000
VERSION     DAKTIP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.432"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..402)
                     /locus_tag="O0305_002956"
     CDS             complement(<1..402)
                     /locus_tag="O0305_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276021.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVVSEIFKIIGLLLLLYQNQYLILVVAQILLG
                     LSYSMMAGVDTAIIKRNITNEKYVQNKSNSYM"
     gene            complement(395..>700)
                     /locus_tag="O0305_002957"
     CDS             complement(395..>700)
                     /locus_tag="O0305_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HCY0276022.1"
                     /translation="IAIGCVQNKTMRRFISYINSPIAITSANISGTADDILITENEAI
                     KHMGENVRYMLRSQNKTNYKTSSTIIKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
BASE COUNT          239 a          107 c           73 g          281 t
ORIGIN      
        1 catatagcta tttgacttat tttgtacgta tttctcattt gttatatttc ttttaattat
       61 tgcggtatca acacccgcca tcattgagta acttaaccct aataatattt gtgccactac
      121 taaaattaaa tattgatttt gatataataa caacaataaa ccgatgattt tgaatatttc
      181 actaacaact aatttattag aatcttttaa gttacaaatt ttaaaacact tctctttgta
      241 tagagagaat aaaaatgctg caatgccata agacgccatt aatatttcta attgtattat
      301 ggaataacct tgaatcaaaa agtaaattaa taaaaacggt aaataaaagt atgctcgtga
      361 aaaaattcgg tataaataaa tcccaagtat taacttattc ataaataata cctgtaccta
      421 gtctttcttt tatttcttca aactttatat ctccttctct taataattca attttattat
      481 ctgtcacttt aataatcgta ctagatgttt tatagttagt tttattttga cttcttagca
      541 tatatcttac attttcgccc atgtgcttaa ttgcttcatt ttcagttatt aaaatatcat
      601 cggcagttcc agatatattt gctgaagtaa ttgctatagg tgaattaata tatgaaataa
      661 atcttctcat cgttttattt tgtacacatc ctatagctat
//
LOCUS       DAKTIP010000425          698 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.433, whole genome shotgun sequence.
ACCESSION   DAKTIP010000425 DAKTIP010000000
VERSION     DAKTIP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.433"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            56..676
                     /locus_tag="O0305_002958"
     CDS             56..676
                     /locus_tag="O0305_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276023.1"
                     /translation="MKFTEVEVIEHLVKAYKEAGKPTYPHENLYRGRNHSISGIGEDL
                     LGAYLISRLEGVQIFIDQPLSMIDKSLSTRYPDLLICEDNEIKNILEVKMDLGYQRKD
                     FIDYCRKKEEWISNIVGKQCVLSRKREDKIPMNIADDIKFHVVIYSENNGPKRFDEEI
                     MPIVNETCPHIEVYVLTSGQHPNLVNVNLEGININKDEFEILVNAL"
BASE COUNT          264 a           76 c          136 g          222 t
ORIGIN      
        1 tgtgatatac tttgtttgtt gaaatataga gtatctatag atagggtgat tgagtatgaa
       61 attcactgaa gtggaagtta tcgaacatct tgtaaaggca tataaagaag caggaaagcc
      121 tacttatcct catgaaaatt tatatcgagg acgtaatcat agtatttcag gtattggaga
      181 agacttgctg ggtgcttatt tgattagtag attggaaggt gtccaaatat ttattgatca
      241 gcctttatct atgattgata aatctttaag tacaagatat ccggatttat taatttgtga
      301 agataatgaa attaaaaata tactagaagt taaaatggac ttaggatatc aaagaaaaga
      361 ttttatagat tattgccgaa agaaagaaga atggatttca aatatcgtag gaaaacagtg
      421 tgtattgtct agaaagagag aagacaaaat tcctatgaat atagctgatg atattaaatt
      481 tcatgttgtg atttacagtg aaaacaatgg accgaagcgg tttgatgaag aaatcatgcc
      541 tatcgttaat gaaacatgtc cacatattga agtatatgtc ctaacaagcg gtcaacaccc
      601 taatttagta aatgttaatc ttgaaggtat taatattaat aaagatgaat ttgaaatatt
      661 agtaaatgcg ttataaaaaa atagagcatc ctccacgt
//
LOCUS       DAKTIP010000426          692 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.434, whole genome shotgun sequence.
ACCESSION   DAKTIP010000426 DAKTIP010000000
VERSION     DAKTIP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.434"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            16..692
                     /locus_tag="O0305_002959"
                     /pseudo
     CDS             16..692
                     /locus_tag="O0305_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          273 a           98 c          113 g          208 t
ORIGIN      
        1 tataaaaatt ataacatgac cccaacttac actaccaata gaaacttcgt tagaattcct
       61 caaaatgata tttcgcgata tgttaatgaa attgttgaaa cgatacctga tagcgaattc
      121 gatgaattca gacatcatcg tggtgcaaca tcctatcatc caaaaatgat gttaaaaatc
      181 atcttatatg catatactca atctgtattt tctggtcgta gaatagagaa attacttcat
      241 gacagtattc gaatgatgtg gttagcccaa gatcaaacac tttcttataa aactattaat
      301 cgttttagag tgaatcctaa tactgatgcg ttaattgaat ctttatttat tcagttctat
      361 agtcaatgtt taaagcaaaa tcttattgat gataattcaa tttttattga tggtacaaaa
      421 gtagaagcta gtgccaatag atatacattg gtgcggaaga aagtattcaa aatcacgaat
      481 cgaaattgaa cgagaattca aaaacactat atcgtgactt agttgaagaa aaaataatac
      541 cagagataaa agaagatgga gatagcgatt taacaataga agaaatagat ttaattggta
      601 gtcatttaga taaagaaatc gaagattgtg ctcaaattag aaaacagacc cgtaaaaaag
      661 aactgaaaga tgagacagta gatgctcatc tt
//
LOCUS       DAKTIP010000427          677 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.435, whole genome shotgun sequence.
ACCESSION   DAKTIP010000427 DAKTIP010000000
VERSION     DAKTIP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.435"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>677
                     /locus_tag="O0305_002960"
     CDS             <1..>677
                     /locus_tag="O0305_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276024.1"
                     /translation="ILMKYLENEINFKEVWKKYIDELFKKLNVQKLLIHKKIESLISS
                     YDKKISDDCSMYSKVFEQYKKNVVLDKVYYSEVYKYSDNLIKVFSEDVWNIMGYKKYN
                     EYFTNRDDTTLKITNLYIGQSFGTEGNRNVIDRIGKGHEKLQEIMTYQPENRKTVLIF
                     YKIQPDNIYITNQYGKEAVSRLKRILEQSIPNKEYIDLSEISLITFFKPEYNKQHVNE
                     EFNSLSSS"
BASE COUNT          297 a           60 c           98 g          222 t
ORIGIN      
        1 attttaatga aatatctaga aaatgaaata aatttcaaag aagtatggaa aaaatatatt
       61 gatgaattat ttaaaaaatt aaatgttcaa aaattgttaa ttcataaaaa aatagaaagt
      121 ttaatatcct cgtatgataa aaaaatatct gatgattgta gtatgtactc aaaagtattt
      181 gaacaatata aaaaaaacgt tgtattggat aaagtatatt actctgaagt ttataaatat
      241 agtgataatt tgataaaagt attctctgaa gacgtttgga acataatggg gtataaaaaa
      301 tataatgaat attttacaaa cagagatgat actacattaa aaattactaa tttatatatt
      361 gggcaatctt ttggtacaga gggtaataga aatgtcattg atagaatagg gaaaggtcat
      421 gaaaaattac aagaaattat gacttatcag ccagaaaata gaaagactgt cttaatattt
      481 tacaagatac agcctgacaa tatttatatt acaaaccaat atggtaaaga ggctgtgtct
      541 cgattaaaaa gaattttaga gcaaagtata cctaataaag aatatattga tttatcagag
      601 atatcattaa ttacattttt taaaccagaa tataataaac aacatgtgaa tgaggagttt
      661 aattctttat cttcttc
//
LOCUS       DAKTIP010000428          665 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.436, whole genome shotgun sequence.
ACCESSION   DAKTIP010000428 DAKTIP010000000
VERSION     DAKTIP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.436"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..625
                     /gene="sek"
                     /locus_tag="O0305_002961"
     CDS             <1..625
                     /gene="sek"
                     /locus_tag="O0305_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type K"
                     /protein_id="HCY0276025.1"
                     /translation="YTKKDFVDLKDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSN
                     FKGKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRNIPINIWINGNHKTISTNKVS
                     TNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNN
                     NDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLDVDISYKETI"
BASE COUNT          273 a           83 c           95 g          214 t
ORIGIN      
        1 ttatacaaaa aaagactttg tagatttaaa agatgtaaaa gacaatgata ctcctatagc
       61 taatcaacta caattttcaa atgtatctta tgatttaatt tcagaatcaa aagattttaa
      121 taaatttagt aatttcaagg gaaaaaaact tgatgttttt ggtattagtt ataatggcca
      181 gtgtaacaat aaatacatat atggtggagt cacagctact aacgaatatc tagataaacc
      241 tagaaatatc cctataaata tatggatcaa tggaaatcac aaaactattt ctaccaataa
      301 agtttcgaca aataaaaaat ttgttaccgc tcaagagatt gatgtcaaat taagaaagta
      361 ccttcaagaa gaatacaaca tttatggaca taacggcact aaaaaaggag aagaatatgg
      421 tcataaatca aaattttatt ctgggtttaa tattggtaaa gtaacgttcc atttaaataa
      481 taatgacact ttttcatatg atttattcta cacaggagat gatgggttac caaaaagttt
      541 tttaaaaatt tacgaagaca ataaaactgt agagtctgag aaattccatt tggatgtcga
      601 tatttcttat aaagaaacga tataaatcta ttaattatgt cataatcatt attttccggg
      661 tagca
//
LOCUS       DAKTIP010000429          663 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.437, whole genome shotgun sequence.
ACCESSION   DAKTIP010000429 DAKTIP010000000
VERSION     DAKTIP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.437"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>663)
                     /locus_tag="O0305_002962"
     CDS             complement(<1..>663)
                     /locus_tag="O0305_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCY0276026.1"
                     /translation="WFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSY
                     LSISRTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIVYVIHPINADWLL
                     GSSPYTSLENEGQSIFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYL
                     LHGLIIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRP
                     SQ"
BASE COUNT          260 a           86 c           96 g          221 t
ORIGIN      
        1 aactgtgatg gtctttgtaa attaattatt ggatttgtcc atttacaaac aaagtttgta
       61 gataatacat atacgatgat tacagatata cttattaaat aaagataagt cattaatgat
      121 ataggattat cgaatgggta ccattcaaat cctctaacaa tgccaataat taaaccatgt
      181 aataaatata cgtatagcgt acgactacca atataagtat ataatttttt ctttgttgac
      241 attaaattta gaaacgcagt cattgcgatt aatataattc catataatat aagtcgttta
      301 aaaggactga atatactctg tccttcattt tcaagtgaag tatatggtga acttcccaat
      361 aaccaatctg cattgatagg atgaatcacg taaacgataa aaaacaaaat aaaggtaatg
      421 atagatactg gtattagttt tttattttta aaaatagccg tatgtttttt ggtgaaaatg
      481 taacctagat aaaatattgg gaaaaatacg attgtccttg aaatgcttaa gtagctatcg
      541 atgttatctg aaaaacctgc tccaatagat ataataattg aaactgatag cactttatat
      601 ggattaaatc ttctaactat tactaaaatg acatgaaaga aaaatagcgt gatcaaaaac
      661 cat
//
LOCUS       DAKTIP010000430          633 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.438, whole genome shotgun sequence.
ACCESSION   DAKTIP010000430 DAKTIP010000000
VERSION     DAKTIP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.438"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(110..>633)
                     /locus_tag="O0305_002963"
     CDS             complement(110..>633)
                     /locus_tag="O0305_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCY0276027.1"
                     /translation="VVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQI
                     ALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNRI
                     ISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAKV
                     VLNANDIFEDYYI"
BASE COUNT          203 a           99 c           95 g          236 t
ORIGIN      
        1 ttttatatct aaacaaatca ttttccctgt ctatttttta tagattttat taaaaaataa
       61 attacaaagg gaaacaaaag aaatatgaag caactcgtta tttttagttt taaatatagt
      121 agtcttcaaa tatatcatta gcgtttaata ctaccttagc accttcttgg atacgtaata
      181 aattaccttt tgtcatagga ttaaacatag atccaggtaa aacataaaca tttctatttt
      241 gctctaatgc aaaatctatc gtgatgtgac tgccactttg ttccttagcc tcagtaatta
      301 aaaccccttt tgacaaaccg ctgataattc tattgcgctc aggaaatcta tatttagcga
      361 ttggtgtatg tggtggatat tcagatataa ctaaaccttt ttcttctatt ttatttctta
      421 atgctaatgt acttttggga taatgtgttt gatggccaaa ggctaaaact gcaattgtag
      481 ggagattgta ttttaaagct atttgatgtg ccattgcatc agctccttga gcaaggccgg
      541 aaacaattgt taaatatttg cttttatcat ttgataataa aaattctaaa gactgttggg
      601 tataacttgt agaatctctt gcacctacta ctg
//
LOCUS       DAKTIP010000431          631 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.439, whole genome shotgun sequence.
ACCESSION   DAKTIP010000431 DAKTIP010000000
VERSION     DAKTIP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.439"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            351..>631
                     /locus_tag="O0305_002964"
     CDS             351..>631
                     /locus_tag="O0305_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0276028.1"
                     /translation="MKREVILKLQQFIIERENADKRRQYKKENEELDLSLTQFHIIEL
                     IDNNDKVNNKFLSEMLNVSKPAITKSIKKLLAKDLVVESHNEFNKREVN"
BASE COUNT          272 a           56 c          111 g          192 t
ORIGIN      
        1 aaaatatgga tttaaaaaac tttaataata taaaacataa atatgaatac gagtattttt
       61 gcagcatgca aatggcattg ctacatttat taaaatgcgc aatttgttat gagtaacttt
      121 tttggatagt aggcacatta aagtgtcgaa aacaatttgt gtggaagaca gggtaaattc
      181 tgcttattta aaatgatagt ttgtattata ggtggatttg tgaaatgcgg aataaatggt
      241 gcataactaa gtaaatatat gttaattaca acaatatttg acaaaaatgg gagtgacaac
      301 aataatatag ttaaccggtt aactaatgaa gaggaggaat aatagtggat atgaagagag
      361 aagttatttt aaaattacaa caatttatta tagaaagaga aaatgccgat aaaagaagac
      421 agtataaaaa ggaaaatgag gaactagatt tatctctaac acaatttcat atcatagagt
      481 tgattgataa taatgataaa gtgaataata aatttttgtc tgaaatgtta aatgtatcaa
      541 aaccagcaat tactaaatcg ataaaaaaac ttttagcgaa agacttagta gttgaatcgc
      601 acaatgaatt taacaaaaga gaagttaatt a
//
LOCUS       DAKTIP010000432          623 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.440, whole genome shotgun sequence.
ACCESSION   DAKTIP010000432 DAKTIP010000000
VERSION     DAKTIP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.440"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(204..557)
                     /locus_tag="O0305_002965"
     CDS             complement(204..557)
                     /locus_tag="O0305_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCY0276029.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
     gene            complement(554..>623)
                     /locus_tag="O0305_002966"
     CDS             complement(554..>623)
                     /locus_tag="O0305_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="may be involved in chromosome condensation;
                     overexpression in Escherichia coli protects against
                     decondensation by camphor; overexpressing the protein
                     results in an increase in supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="camphor resistance protein CrcB"
                     /protein_id="HCY0276030.1"
                     /translation="VTSYVFGILLCYVGIKLGGDLS"
BASE COUNT          233 a           93 c          100 g          197 t
ORIGIN      
        1 ttttttaggg atttatgtcc cactcccttt attttcgact agtttggaga atttattgac
       61 attcacatta tttaaacggc aacaaagatt attgaaattt ggtaggcatt atatggtgct
      121 taaaaatttg catgaaaatt aaaaaatgct tcgttcagga aggtgtcgta atttacctat
      181 ttgctgaatg aagcatttta tttttaaata tgatagccaa tataacaagc tataaatcca
      241 atgatgaatt gtaaaagtga ataattgaga aaaaggttaa tattaaattt tggcgtcatc
      301 attagtgtaa gttctttggc taacgttgag aaagttgtta agccacctaa aaaaccggta
      361 acaaagaacg cagggaacca tgagattgaa attgataagc ctatagttaa tccaattaaa
      421 aaactaccaa ctagatttac tatcaatgtt gcgataggta actttgaagt aaatttatga
      481 ttaaaataat cagtaatggc acttctagca attgcgccaa aaccgccgcc aatcatgact
      541 aaaatgattg atatcatgat aaatcaccac ctagttttat accgacgtaa cataacaaaa
      601 taccaaagac ataacttgtt aca
//
LOCUS       DAKTIP010000433          621 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.441, whole genome shotgun sequence.
ACCESSION   DAKTIP010000433 DAKTIP010000000
VERSION     DAKTIP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.441"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(333..566)
                     /locus_tag="O0305_002967"
     CDS             complement(333..566)
                     /locus_tag="O0305_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276031.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          219 a           94 c           66 g          242 t
ORIGIN      
        1 agctatcttt agttttaata taaactatta ggataatcga cgtaagaaga atcatatata
       61 ttaatgatga agaagtccat acaaaatccg catcatttgt tgttaataat gggactataa
      121 ttaatccgaa attaatcatg catgctatat atactataat gttatacaca atgttaattt
      181 ttgttcacca ccttatactt ctattttaaa aacttcttta taatgatata ttgtttaatg
      241 ttgaaataat tagattatct aattttcatt tgctttacat gtaaaaggct atatatagta
      301 tgctctttat gattctaaat gctttttaat atttaatgct catcaacatt tggattttga
      361 atattcaatt caaaaacttt attagctacg tcaattgtaa aatcagaacc atagttgaca
      421 tgagctactt ttaattttcc atctaaataa tagattgcga ttgcaacatc gtaaaattcg
      481 tcaatgacaa ataaactctt ttcgtttgtt acaacctcat gctctcctga gtatacaacg
      541 ttaatttccc aatcattaaa aaccatttgt taacctcctt gaacatttaa attgattcaa
      601 cttaagttta acttattcat a
//
LOCUS       DAKTIP010000434          610 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.442, whole genome shotgun sequence.
ACCESSION   DAKTIP010000434 DAKTIP010000000
VERSION     DAKTIP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.442"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            20..>610
                     /locus_tag="O0305_002968"
     CDS             20..>610
                     /locus_tag="O0305_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCY0276032.1"
                     /translation="MTTFDAKKLKKEYLDWYNQTLEFSNLSNNVVRIDTPFKDNSLDN
                     LIIYALYDQSRDMITLTDDGYTIFDLENNGISLNKSKKRKKIFEEHLSAYGIKYNDKT
                     HEIFVQTNFKNFNKSKHNLLQCLIFVNDMYLLSNPKSQNIFTEDVANKLDEHNIYYGR
                     DLPIIGSSGVVHNFDFFISAKKNQKEKFINAISNPNN"
BASE COUNT          244 a           77 c           81 g          208 t
ORIGIN      
        1 tgaataaagg agggataata tgactacatt tgatgctaaa aaattaaaaa aagaatatct
       61 tgattggtat aatcagacct tagagttttc taatttatca aacaatgtag taagaataga
      121 tactcctttt aaagataatt ctttagataa tttaataatt tacgctttat acgatcagtc
      181 cagagacatg attacactga cagatgacgg ctatactata tttgatttag aaaataatgg
      241 tatttcttta aataaatcaa aaaaacgtaa aaagattttt gaagagcacc tttcagctta
      301 cggtattaaa tataacgata aaactcacga aatttttgtt caaactaact ttaaaaattt
      361 taataaatcg aaacataatt tattacagtg ccttatattt gttaatgata tgtacttact
      421 ttctaatcct aagtcacaga acatatttac agaagatgtt gcaaacaaat tggatgaaca
      481 taacatttat tacggaagag atttacctat tataggaagc agtggtgttg ttcataattt
      541 cgactttttt attagcgcta agaaaaatca aaaagaaaaa tttatcaatg ctatttctaa
      601 ccctaataat
//
LOCUS       DAKTIP010000435          609 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.443, whole genome shotgun sequence.
ACCESSION   DAKTIP010000435 DAKTIP010000000
VERSION     DAKTIP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.443"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            70..288
                     /locus_tag="O0305_002969"
     CDS             70..288
                     /locus_tag="O0305_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276033.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            420..>609
                     /locus_tag="O0305_002970"
     CDS             420..>609
                     /locus_tag="O0305_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCY0276034.1"
                     /translation="MTFEEKLSEMYSEIANKISSMIPVEWEKVYAMAYIDDGGGEVFY
                     YYTEPGSNELYYYTSVLNK"
BASE COUNT          239 a           56 c          121 g          193 t
ORIGIN      
        1 atctttttta ctataattat aaatagttaa ataaattgaa agacaaaata atgagtagga
       61 gttaatgaga tggaaaacca aaaacaaggc aatggcttaa aaattgcaac atgggtattt
      121 attgtattaa cagtagttac accgctattt ggtattggaa gtattgtttg tagtattaat
      181 tataaaaaat acgatgctga aaaaggttcg aagttattgc aaattgcaat tatcgtaaca
      241 ataattgctt ttgttttaaa tttattagca tatttaggtt taagataagt aaaacgaatt
      301 tgaagaagca ttaagcgaca tttgggtgtt gtttaatgct ttttatttaa cggaaataag
      361 tgtagagaat aaattaatag tttaatcaag agatatttcg aacacatagg ggcgataaca
      421 tgactttcga agaaaaatta agtgaaatgt atagcgagat tgcgaataag attagcagca
      481 tgataccggt agagtgggaa aaggtatatg caatggcata tatagatgac ggaggaggag
      541 aagtgttcta ctattacaca gaacctggaa gtaatgaatt atactactat actagtgtat
      601 taaataaat
//
LOCUS       DAKTIP010000436          571 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.444, whole genome shotgun sequence.
ACCESSION   DAKTIP010000436 DAKTIP010000000
VERSION     DAKTIP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.444"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..507
                     /locus_tag="O0305_002971"
     CDS             <1..507
                     /locus_tag="O0305_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCY0276035.1"
                     /translation="KTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEGTKY
                     LAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTSGLL
                     AEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKIDQTS
                     INLIGRKI"
BASE COUNT          215 a           73 c          102 g          181 t
ORIGIN      
        1 aaaactgaat ttaaagggaa caatatacca tatgaatacg cagcaggtgc agatgtgagt
       61 gattctatta acgggaatcc aattaagtca tttccatttg aagtaattga attaccggaa
      121 gggactaaat atcttgcttg gtctttaatt gactatgatg caattcctgt atgtggcttt
      181 gcttggattc attggagtgt agctaatgta agtgttagtg gcaattcaat ttctataaaa
      241 gcagatttat caagaacaaa gggcgactat gtacaaggta aaaatagctt tactagtggg
      301 ttgttggctg aagatttttc agaaatagaa aatcactatg taggacctac accacctgat
      361 caagatcatc aatatgaatt aacagtttat gcgttagatc attctttaaa tttgaagaat
      421 gggttctact tgaatgaatt tttaaaagaa gtaaatcaac ataaaattga tcaaacaagt
      481 attaacctta taggaagaaa aatttaatac taaatatctc atcaatataa aattgttcaa
      541 ttaaaagtac aaagaaacaa aggttttaat t
//
LOCUS       DAKTIP010000437          558 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.445, whole genome shotgun sequence.
ACCESSION   DAKTIP010000437 DAKTIP010000000
VERSION     DAKTIP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.445"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(30..536)
                     /locus_tag="O0305_002972"
     CDS             complement(30..536)
                     /locus_tag="O0305_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0276036.1"
                     /translation="MAHIIRRVSIKDVENFISMLANIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
BASE COUNT          178 a           93 c           98 g          189 t
ORIGIN      
        1 ttctgataca agattatgat aaaatagctt taattaagaa ttttagccat catatagtca
       61 tcataatatt taccatcgat aaataactta tcttttaaaa cgccttcgat ttgaaaatcg
      121 gcacttttaa aaagctcgag ggcaggttgg ttattgagtg gtacatttgc ttcaattcgg
      181 tgtatttgat tgtttaaaca ccaagccata atggcatcaa gaagtgcttg gccaattcca
      241 cgatgttgat ataatttctt tacacctaaa tcaattttag caacatgttt aatgcgttga
      301 aatggtgtcg tattaacaaa ggcaaagcca acgagttgtt catcactttc agcaacgaag
      361 atgactttat gcggagaagt gatatattct tctaattgtt tactagccga tgtgacgcta
      421 ggatcatatt ctcctggtgt gtagaacata tacggagatt cgtcgtatat gttcgctaac
      481 attgaaatga aattttctac atctttgata ctaactctac gtataatatg ggccataaaa
      541 agcctccagt attttgaa
//
LOCUS       DAKTIP010000438          556 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.446, whole genome shotgun sequence.
ACCESSION   DAKTIP010000438 DAKTIP010000000
VERSION     DAKTIP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.446"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            147..323
                     /locus_tag="O0305_002973"
     CDS             147..323
                     /locus_tag="O0305_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCY0276037.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            338..>556
                     /locus_tag="O0305_002974"
     CDS             338..>556
                     /locus_tag="O0305_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCY0276038.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIIL"
BASE COUNT          189 a           57 c           96 g          214 t
ORIGIN      
        1 tttgctcaaa atgccaatta tatactcaca aatcattcta aatatcggct taaagttaca
       61 ttttattatt tttgattgtt aatagtttat ttgttaagtg aattttagta caatataatt
      121 tagttatgat ttaaggagga cttaaaatga ctgttgcaga agtgggtaac attgttgagt
      181 ttatggatgg attaagaggt cgtgttgaaa aaatcaacga taactctgtt attgttgact
      241 taacaattat ggaaaatttt aatgaccttg atttaccgga aaaaactgtt atcaatcata
      301 aacgatataa gattgttgaa taagaaggta agttataatg aataaaatct cgaaggcttt
      361 aacttggttt attataagtt tcattatatt tcatctcata ttatttatta tgtggggcga
      421 acaccaagaa tactggtatt tatatacagg tataatgcta attgctggta tcagttatgt
      481 attttatcaa agagatattg aatctaagcg gttgcttaca tcaattggtg ttggtattat
      541 tacggcaatt atttta
//
LOCUS       DAKTIP010000439          545 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.448, whole genome shotgun sequence.
ACCESSION   DAKTIP010000439 DAKTIP010000000
VERSION     DAKTIP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.448"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>545)
                     /locus_tag="O0305_002975"
     CDS             complement(<1..>545)
                     /locus_tag="O0305_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276039.1"
                     /translation="IKWPIIMTGIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLP
                     EEKFWILHYSFLRALILGFFIGFIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLT
                     KYLNYKFVSEICLVIFLIIYTYQSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRV
                     MLNYAETLYFIFNIAFLLMGG"
BASE COUNT          221 a           77 c           54 g          193 t
ORIGIN      
        1 taaccaccca taagtaaaaa tgcgatatta aaaataaaat ataacgtttc tgcataatta
       61 agcatcactc ttaagttact actgctagaa agtttattta tagtttgtgg acgagttaac
      121 cctgaagaaa tacctaaaaa tatcatagaa atagtaactg ctatgaaact ttgatatgta
      181 tatattatta aaaatattac taaacaaatt tctgacacaa acttataatt caagtattta
      241 gttaaataac gtgaagatac aaaacccata actgtgtaac aagttaatac tgaaataaat
      301 tgtaaattat tcagttttaa atcattatat atattaattg gaataaatcc tataaaaaat
      361 cctaatatta acgctcttaa aaaagaataa tgcaatatcc aaaacttctc ttctggtaga
      421 aatttcttta tctttccctt tgtttctcct attaaattta attccctatt ttcaactaat
      481 gtgcatcgaa taattataat tgttagaatt gaaaatatac cagtcattat tataggccat
      541 ttaat
//
LOCUS       DAKTIP010000440          521 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.449, whole genome shotgun sequence.
ACCESSION   DAKTIP010000440 DAKTIP010000000
VERSION     DAKTIP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.449"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..450)
                     /locus_tag="O0305_002976"
     CDS             complement(<1..450)
                     /locus_tag="O0305_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0276040.1"
                     /translation="MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDK
                     LPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYV
                     FGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAI"
BASE COUNT          217 a           96 c           50 g          158 t
ORIGIN      
        1 tatggcatta actgcaggat taggattttc actatcaaat tcgtttttat aaaataaaaa
       61 taaacctaac caagaaataa ataacatcaa gataatacct agacaataat tgaaaacatc
      121 aaatccaaaa ccgactacac caaaaacgta aatagataca aaaagcaatg gcaaagtata
      181 tcccacaata acgttaagtg tgagatatct aaccacatat cttttttcaa ctttgtaatt
      241 ttcatttttc tcttgtatgt acatcataat caaaattaca gctgtaaagg ccataaacac
      301 cagtaaatac ataggcaatt tatcatatag tttagcgtag aaaaacataa tcgcgtcaaa
      361 aaataatgaa actgcaacta caaatactcc caatacagtc aagtatggtc tttttatatt
      421 ctttacaaaa tcatcgactt tcaattccat aaattcataa tctccccaat cttacttcaa
      481 tatttttata agttaaataa atcttcttcg ccaaaaaaat g
//
LOCUS       DAKTIP010000441          520 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.450, whole genome shotgun sequence.
ACCESSION   DAKTIP010000441 DAKTIP010000000
VERSION     DAKTIP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.450"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..264)
                     /gene="crcB"
                     /locus_tag="O0305_002977"
     CDS             complement(<1..264)
                     /gene="crcB"
                     /locus_tag="O0305_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCY0276041.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFD"
BASE COUNT          187 a           78 c           87 g          168 t
ORIGIN      
        1 atcaaacata tgtattaatt ctaattgaaa tgttgaaaaa gtcgttaaag caccaagaaa
       61 accagtcgta atagcttttt ttagggtcgg atggtttgaa aaaaatgcaa ttgttaaggc
      121 tgttagcaat cccattacaa aggcaccagt caaattggct atcagtgttc cgattggaaa
      181 acctccgtca gtattcagaa aagaaatgag gtaacgtaat aaagcgccta aagcaccacc
      241 gataaaaata tatacatatt gcatttggtt cacctcgaaa agaagtagtt tgaatttaaa
      301 aaagaggttt tggcaacgcg acgacaaaaa ttgtcgatgc attatcaaac ctcattatat
      361 gttatatctt gttgtataac tatagcgatt agatgcatag ttatgatttc gaaaatctaa
      421 tattttttat acgcaacaac gttatcaaat tgttttactc attatagcat gatacattgt
      481 attgttttgt attaaggcta caatgacata ttaacttttt
//
LOCUS       DAKTIP010000442          513 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.451, whole genome shotgun sequence.
ACCESSION   DAKTIP010000442 DAKTIP010000000
VERSION     DAKTIP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.451"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            292..>513
                     /locus_tag="O0305_002978"
     CDS             292..>513
                     /locus_tag="O0305_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCY0276042.1"
                     /translation="MKKEKRLNLILTVIQQNQFNKKQQIVDYMARHFGVYYSLTTISR
                     DLQELEIYKIPVENKKYIYKKINQTNQLSA"
BASE COUNT          205 a           66 c           53 g          189 t
ORIGIN      
        1 tactttttat ttcaatgcct ttttccaaac ttcttttatg agatattgtg gttattacct
       61 gacatttaca atttacacat ttcgctatta ttacgcaaat tattttgatt ttataattat
      121 gatgactata atctattcta attaatctct ccttttaggc ataaaataat ctacttataa
      181 ttaaagcatc cactatttca aaattatttg taaattgaga tttataagtg atttttatac
      241 agttatttaa aatctatttc ataatcaaaa ataataaagg gggtaataaa aatgaaaaaa
      301 gaaaaacgtt taaatcttat cttaactgtt atacaacaga atcagtttaa taaaaaacaa
      361 caaatagtgg attacatggc aagacatttt ggagtttact atagcttgac aactatttct
      421 cgtgacttac aagaattaga aatttacaaa atccctgttg aaaataaaaa gtatatttac
      481 aagaaaataa atcaaacaaa tcaattaagt gca
//
LOCUS       DAKTIP010000443          505 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.452, whole genome shotgun sequence.
ACCESSION   DAKTIP010000443 DAKTIP010000000
VERSION     DAKTIP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.452"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            1..505
                     /locus_tag="O0305_002979"
                     /pseudo
     CDS             1..505
                     /locus_tag="O0305_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT          213 a           47 c           87 g          158 t
ORIGIN      
        1 tttagaacta agtaatatga tactcttgtt agatgatatg ctcgacgtca atatatatat
       61 ataaattctt ttaaaaataa tacattagag acatttgatg cgactgatcc tgaagttgat
      121 tacatcactg aaataggaga ttacttatta aaatatgacg ttaattctat aaatgatgtc
      181 agaattgaag tgatagataa tttggggcat tcaaataata tgctactaaa aacgatacta
      241 catgcagaag ttgaaatgga agatggaaag aaatatttat tacaaaatgg gaaatggggt
      301 tattttaata gagaattttt tgaccttttg aatgatcatt tgaatgagat agaaattagg
      361 tataacacac taactcccac aggtttagta tttaaagaag gagaagaagg atatataaaa
      421 gaaatagtag gaagattgcc agaagaatat ttaatgttac ataaaaaatt cataaaacca
      481 ataaataaga attttatagt aaaag
//
LOCUS       DAKTIP010000444          489 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.453, whole genome shotgun sequence.
ACCESSION   DAKTIP010000444 DAKTIP010000000
VERSION     DAKTIP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.453"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          176 a           84 c           91 g          138 t
ORIGIN      
        1 acgaagttat attggcagta gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca
       61 attctagtca ggggccccaa cacagagaat ttcgaaaaga aattctacag gcaatgcgag
      121 ttggggtgag ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
      181 tggggatggg ccccaacaaa gagaaattgg attcccaatt tctacagaca atgcaagttg
      241 gggtgggacg acgaaataaa ttttgcgaaa atatcatttc tgtcccactc ccatcaaaag
      301 aatgacattg aaaaaaatat taaagtttgg tgccataatt ttctttctgc tcaatgaaat
      361 aatacgttta aaaattgaat tgaatcattc gcgaaattga taattattct caattaataa
      421 aaaatactta ttacacctta cctgtatgat aagttttgct tatatactct gattaaaaag
      481 tcaaaacct
//
LOCUS       DAKTIP010000445          487 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.455, whole genome shotgun sequence.
ACCESSION   DAKTIP010000445 DAKTIP010000000
VERSION     DAKTIP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.455"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>487
                     /locus_tag="O0305_002980"
     CDS             <1..>487
                     /locus_tag="O0305_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0276043.1"
                     /translation="FSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTMLQF
                     AIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSALA
                     NIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAIIL
                     LY"
BASE COUNT          153 a           63 c           81 g          190 t
ORIGIN      
        1 tttagtttac taacttttcc attagatatt attaatgaaa aacaaaatga atggcgccaa
       61 agattaatgg taacaccatt tacttttact agttattata tttcaaaagt agtgaaaact
      121 atgctgcaat ttgcaatagc gatattagtt atttttatgg ttggacattt ttataaaggt
      181 gttgcaatga gtgcagttca atggttagag tcaggaatat ttttatggtt aggtgcgtct
      241 ctattaataa cttttggcat attattttct ttgttaaatg atattcaaaa aacaagtgct
      301 ttagctaata tcgtaacaat tggtttagca gtattaggtg gattgtggtt tccgataaac
      361 acatttccaa attggcttca acatgttgct catgttttac cgagctatca tttgcgtaaa
      421 ctaggtgtag atattgcttc aaatcatcat atcaatttaa tatcatttgc tataatactc
      481 ttgtatg
//
LOCUS       DAKTIP010000446          475 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.457, whole genome shotgun sequence.
ACCESSION   DAKTIP010000446 DAKTIP010000000
VERSION     DAKTIP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.457"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          204 a           67 c           56 g          148 t
ORIGIN      
        1 agaaaaacaa aaaaaggtaa gcaccgaaat gcttacctac ttcccataaa caatataaca
       61 catatacatt gatttggaaa gcgcaaaaat aaatttaata tactagcccg aaggggagca
      121 atacataaaa aatgaaaggc gctccttgaa aacgcccaag gtaatattaa cataaaatgg
      181 ctactattgc attatctaat tttatttaat taaaacaaat atatatagca caaaaaaact
      241 agcccgaagg ctagcttatg aatagatgaa aatttgaaca cattgctgtg tctaagataa
      301 atatagcaca tattaattac tattaataga aaaattatag cgttttttag attatttcaa
      361 tttatcgaga ccaaattcat caaaacacta attgaatttt aatttttagt taaaaataat
      421 taatctttta ttaatttcaa gttaattaat atatttcgtc gattagatta aactc
//
LOCUS       DAKTIP010000447          474 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.458, whole genome shotgun sequence.
ACCESSION   DAKTIP010000447 DAKTIP010000000
VERSION     DAKTIP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.458"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>474
                     /locus_tag="O0305_002981"
     CDS             <1..>474
                     /locus_tag="O0305_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0276044.1"
                     /translation="TFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASIN
                     KSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHI
                     KLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHG"
BASE COUNT          165 a           74 c           68 g          167 t
ORIGIN      
        1 acattctttt tattagaaca gtttaatact atgcaacata tgggactagt tgaaaaaatc
       61 ggaaattctt ttttccaatc agtaacaaca cgaacagcgg gttttaacag tatagatata
      121 gcaagcatta acaaatctac cgcattaatg ttaatgctac ttatgtttat tggtggtgcc
      181 cctctcagtg cagctggagg aattaaaata actacttttg cagttgcgtt tatttttgta
      241 ctaaattata tacgtaaaga aaataatgtt tcagtattca ataaagaaat atctgacaaa
      301 catataaaac tatctattgt taccattaat atctcatttc tatttatcag catcattact
      361 tttatattat cgataattaa tccgaacata tcattaatca agttattatt cgaagtggtt
      421 tctgcattcg gaacagtagg gttaagtatg aaccttacca cagaatatca tggt
//
LOCUS       DAKTIP010000448          474 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.459, whole genome shotgun sequence.
ACCESSION   DAKTIP010000448 DAKTIP010000000
VERSION     DAKTIP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.459"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            148..>474
                     /locus_tag="O0305_002982"
     CDS             148..>474
                     /locus_tag="O0305_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0276045.1"
                     /translation="MLCETENINKNPKYRIIKYKDEYLMIDLVSTWLALFFPMINWLI
                     PKKYVKISEKDFETLNIVKTAKINSFWPVAGSTVLFGVMLRRYSHLFIVKYEYSIVIL
                     ICCIIIL"
BASE COUNT          181 a           56 c           75 g          162 t
ORIGIN      
        1 cgcgttataa tgaaaaataa ttgattacat ttaaaatatt ctaaatgttg tcgacacaat
       61 ccttttaaga cgctagtaga atttaaatga cttctaatgt atatgaaagt gtatcaatat
      121 aaaaccaatt gaaaagaagt ggagacattg ctttgtgaaa ctgaaaatat taataagaat
      181 cccaaatata gaattatcaa atacaaagat gaatatttga tgattgattt agtaagtaca
      241 tggttagcac tctttttccc aatgattaat tggctgattc caaaaaagta cgtcaaaatc
      301 agcgaaaaag attttgaaac tttaaacatt gtgaagacag ctaaaatcaa ttctttttgg
      361 ccagtggcag gaagtacggt cttatttggt gttatgttaa gaaggtattc ccatttattt
      421 atcgttaaat atgaatatag tatagtaatt ttaatttgtt gcatcataat acta
//
LOCUS       DAKTIP010000449          467 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.460, whole genome shotgun sequence.
ACCESSION   DAKTIP010000449 DAKTIP010000000
VERSION     DAKTIP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.460"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>467)
                     /locus_tag="O0305_002983"
     CDS             complement(<1..>467)
                     /locus_tag="O0305_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HCY0276046.1"
                     /translation="LFSESGHHFDAILFLKEDTTLQNNVYIIPFGQAYHDINNLIDYD
                     FGIDFAERAIKNEDIVNKNVNFFQQNRLKEIVNYRRNSVDYVRPSESYISVQGHPQNP
                     QIFGKTMTCGTSISLRVPNRKQQFIDKISVIIKEINAIINLPQKISEFPRIVT"
BASE COUNT          153 a           68 c           61 g          185 t
ORIGIN      
        1 aaagttacta ttctaggaaa ttcactaatt ttttgaggaa gattaataat agcgtttatt
       61 tctttgatta tcacactaat tttatctatg aattgctgct ttctattcgg tacacgcaat
      121 gaaatacttg taccacaagt cattgttttt ccaaaaattt gaggattctg tggatgtcct
      181 tggactgata tataagattc tgaaggtcta acgtaatcta cactattcct tctataatta
      241 acaatctctt taagcctgtt ttgttgaaaa aaattaacat ttttattaac tatgtcttca
      301 tttttgattg ctctttctgc aaaatcaatt ccgaagtcat aatcaatcaa attatttata
      361 tcatgatatg cttgtccaaa aggtataata tatacattat tttgtaatgt tgtatcttct
      421 ttgagaaata atattgcatc aaaatgatga ccggattctg aaaataa
//
LOCUS       DAKTIP010000450          456 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.462, whole genome shotgun sequence.
ACCESSION   DAKTIP010000450 DAKTIP010000000
VERSION     DAKTIP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.462"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(80..>456)
                     /gene="icaR"
                     /locus_tag="O0305_002984"
     CDS             complement(80..>456)
                     /gene="icaR"
                     /locus_tag="O0305_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCY0276047.1"
                     /translation="IMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNTPEEF
                     SGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQKFGA
                     VEESKSQFKDEVYSLLNIFLKK"
BASE COUNT          162 a           69 c           46 g          179 t
ORIGIN      
        1 actttatata gatttaaaaa aataatctca tgtctttttt tacaaaagta agttaattat
       61 tacaaactag taacaaaaat tatttcttca aaaatatatt tagtagcgaa tacacttcat
      121 ctttgaattg acttttactt tcttccactg ctccaaattt ttgcgaaaag gatgctttca
      181 aataccaact ttcaagaaac agcaatatta aattctgaaa gtcttctttt gtcatcttta
      241 tctttgattc atcatagaat tttgctatct ctttacttaa tgattgattt aaatcttgta
      301 tttgtccgta aatatttcca gaaaattcct caggcgtatt agataattga acgtacattc
      361 taatatacct ttcttcgatg tcgaaaataa actcaaataa gaattgatat aaagcatcaa
      421 ttgaatagtt cgatttattt tgattcatca taataa
//
LOCUS       DAKTIP010000451          454 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.463, whole genome shotgun sequence.
ACCESSION   DAKTIP010000451 DAKTIP010000000
VERSION     DAKTIP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.463"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..425
                     /locus_tag="O0305_002985"
     CDS             <1..425
                     /locus_tag="O0305_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCY0276048.1"
                     /translation="SFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKEMQNV
                     PYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKSAVYT
                     ITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT          181 a           70 c           79 g          124 t
ORIGIN      
        1 aatcatttat aactgtaact ttggcactgg gcatgatcgc aacgactggc gctactgtgg
       61 caggtaatga ggtatctgca gcagaaaagg acaaactacc ggcaactcaa aaagctaaag
      121 aaatgcaaaa tgttccatat acaattgcag tagatggcat tatggctttc aatcaatctt
      181 acttaaattt accaaaagat agccaattat catatttaga tttaggaaat aaagttaaag
      241 ctttgttata tgatgaacgc ggtgtaacac ctgagaagat tcgaaatgca aaatctgccg
      301 tttacacgat tacttggaaa gatggtagta aaaaagaagt ggatcttaag aaagatagct
      361 acacagcaaa cttgtttgat tcaaattcaa ttaaacaaat tgatattaat gtaaaaacta
      421 aataaacact taaaattatc aaattcaaca tgaa
//
LOCUS       DAKTIP010000452          454 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.464, whole genome shotgun sequence.
ACCESSION   DAKTIP010000452 DAKTIP010000000
VERSION     DAKTIP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.464"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>454
                     /locus_tag="O0305_002986"
     CDS             <1..>454
                     /locus_tag="O0305_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCY0276049.1"
                     /translation="YQYKKGQVIYHSTDQIKFVYFLVNGCILHESSNITGDNYLRLSK
                     DENIFPMNFIFNETPAPYEICTALTDCKILTLPKDLLEYLCRKHNEIFESLFKKLNET
                     IQFQVEYIMALRANSAKERIERILQILCLSIGDDNGEFYELKQIMTVQL"
BASE COUNT          184 a           53 c           63 g          154 t
ORIGIN      
        1 tatcaatata aaaaaggaca agtcatatat cattcaactg atcaaataaa atttgtatac
       61 tttttagtaa atggttgtat tttacatgaa tcttctaata ttactggtga caattattta
      121 agattaagta aagacgaaaa tatatttcca atgaacttca tatttaatga aacccctgca
      181 ccatatgaaa tatgtacagc tttgacagat tgtaaaatat taactttacc gaaagattta
      241 cttgagtatt tatgtagaaa gcataatgaa atatttgaaa gtctcttcaa gaaacttaat
      301 gagactattc aatttcaagt agaatatatt atggcgttaa gagctaattc agctaaagaa
      361 agaattgaaa gaatactaca aattttatgc ctttcaattg gggatgataa tggagaattc
      421 tatgaattaa aacaaattat gactgttcaa ttaa
//
LOCUS       DAKTIP010000453          449 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.465, whole genome shotgun sequence.
ACCESSION   DAKTIP010000453 DAKTIP010000000
VERSION     DAKTIP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.465"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            130..>449
                     /locus_tag="O0305_002987"
     CDS             130..>449
                     /locus_tag="O0305_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCY0276050.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTIT"
BASE COUNT          127 a           69 c           72 g          181 t
ORIGIN      
        1 tttgatttaa acaacacatg tgttttgttt ctgctatgta caacatattt catatagact
       61 ctttattatt ttttgtgcta aaatatttac ttgtaaatta tttcgtgttt taaaaaaagg
      121 agtaactcta tgaataaaaa tacatgggtc attgggttta cgctcttcgc tatgtttttt
      181 ggcgcaggca atctaatttt tcctcctaat ctcggattgg atagtggtca atttttctgg
      241 cccgccattt tagcgtttgt tctaactggg attggtttac cattattagg tgtgattgta
      301 ggtgcacttg ataaagaagg atatattggc gcattaaata aaatttcacc taaattttca
      361 atattgttct taatcatcat ttatttgact ataggaccac tttttgcaat acctagaact
      421 gcatctacat cttttgaaat gacaattac
//
LOCUS       DAKTIP010000454          447 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.466, whole genome shotgun sequence.
ACCESSION   DAKTIP010000454 DAKTIP010000000
VERSION     DAKTIP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.466"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          121 a           94 c           59 g          173 t
ORIGIN      
        1 ttcaataaag tatataaaag agcagtaaga tcattttcaa ttagataccg tcttactgct
       61 cttctctatt tatacaatat tttgttttcg atgatatcat tttaaattta tataaacaca
      121 aaattaaagt tgtacatact tagacaatcg aaagtgtaca ttattaaatt atcatttcca
      181 gttctactct agcggaacgt aagttggcta ccatcctcgc taagaacctt tcttgacttg
      241 tgacaatcgc ttgcttcttt cctctccttc ggctctcact tactcattta gctctactaa
      301 actcgttgcg ttcttttctc gtttcgtcag attcaaacgt tttcacttcg ccaagccatt
      361 tttctttgtg tttgcttttt attttgacgt tttagacata aaaaaaagag accttgcggt
      421 ctcaatgcgg ctcatcgcat ccatttt
//
LOCUS       DAKTIP010000455          434 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.467, whole genome shotgun sequence.
ACCESSION   DAKTIP010000455 DAKTIP010000000
VERSION     DAKTIP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.467"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..424)
                     /locus_tag="O0305_002988"
     CDS             complement(<1..424)
                     /locus_tag="O0305_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCY0276051.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLT"
BASE COUNT          122 a           90 c           61 g          161 t
ORIGIN      
        1 aggttaactt ttttgtcatt tcaatatgct cgatgccttc ctcaagaaat atattacctc
       61 tcattttaaa gtttaaactt tcataaaatg ggatagcatg acattgggca ttcatagtag
      121 ctacgtaaaa accttcatct ttagctaatg attctacagc ttgcataagc attctaccca
      181 ttccttgtcc acgatgtgat ttcatcacag ctactcgttc tattttgaca gttgtttcat
      241 taatagggcg tattcgagca gtggcaactg gctgtccatt atcatatcca atgaggtgaa
      301 tagattcaga ttcatattca tcaatttcac tttcctcagg gacgccttgt tcttctacaa
      361 acactttctt tcttatatag aagcaatctt ctaacatctt ttgattgttt acttttgaaa
      421 acataaaatt cctc
//
LOCUS       DAKTIP010000456          428 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.468, whole genome shotgun sequence.
ACCESSION   DAKTIP010000456 DAKTIP010000000
VERSION     DAKTIP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.468"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            164..>428
                     /locus_tag="O0305_002989"
     CDS             164..>428
                     /locus_tag="O0305_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCY0276052.1"
                     /translation="MNKKYLMIVIIILILILGGIFLKMKYDEKKYYDEQKERITIYMK
                     YNVKGYKNISFANFKENPMDGYSISGYINNDKKLSFTAGIRSVD"
BASE COUNT          183 a           38 c           66 g          141 t
ORIGIN      
        1 agtaaaccaa atcatgattt aagattataa gaaagaaaaa ttgactaatg ttaaaaccaa
       61 caatcatatg ggttgtaacg tttatacaat catcgtaaaa ctcaatatgc ttaatttatt
      121 caaaatgttc gtcaataagt acaatgaaaa ggagattgat tttttgaata aaaaatatct
      181 tatgattgta attataattt taatattgat tctaggcgga atctttttga agatgaaata
      241 tgacgaaaag aaatattatg atgaacaaaa agaaagaata acgatttata tgaagtacaa
      301 tgtgaaaggt tataaaaata taagcttcgc taattttaaa gaaaacccaa tggatggtta
      361 ttctattagt ggttatataa ataatgataa aaagttatca tttacagctg gtataagatc
      421 tgttgatg
//
LOCUS       DAKTIP010000457          427 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.469, whole genome shotgun sequence.
ACCESSION   DAKTIP010000457 DAKTIP010000000
VERSION     DAKTIP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.469"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            192..427
                     /locus_tag="O0305_002990"
                     /pseudo
     CDS             192..427
                     /locus_tag="O0305_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          168 a           63 c           62 g          134 t
ORIGIN      
        1 aattttctgt gttggatccc ttcgtataat ttaataaata ctactaaact aaattaacga
       61 ggtgccttat gtataaaaat tataacatga cccaacttac actaccaata gaaacttctg
      121 ttagaattcc tcaaaatgat aatacgcgat atgttaatga aattgttgaa acgatatctg
      181 atagcgaatt cgatgaattt atatgtccta ataacaaaag aataggtttt aaaagatatg
      241 cataccgtaa tgatagatat agttttaaac gtgacttcaa gctatatgaa tgtgatgact
      301 gttcatcatg ttctttgaga catcaatgca tgaagccaaa ttcgaaatcc aataagaaaa
      361 ttatgaagaa ttataattgg gaatacttta aagcccaaat taatcaaaag ctttctgaac
      421 cagaaac
//
LOCUS       DAKTIP010000458          425 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.470, whole genome shotgun sequence.
ACCESSION   DAKTIP010000458 DAKTIP010000000
VERSION     DAKTIP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.470"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            20..>425
                     /locus_tag="O0305_002991"
     CDS             20..>425
                     /locus_tag="O0305_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCY0276053.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITTIIELIIIVISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITVVFALC
                     NLYYFAAVQNHYDLFNYWLMGSISIIISYLLLV"
BASE COUNT          146 a           44 c           63 g          172 t
ORIGIN      
        1 taatgtatag gagatagata tgactactaa agaaaatatt gatactcttc gaaagccagg
       61 cgcacaagcc ttaagtttaa tatcattgtt tttgatactt ttttcatgtt tgactttctt
      121 ttttggttta gattatgaaa ggtttccaaa ttatttaaag ataacgacaa ttatagaatt
      181 aataattatt gtaattagtt tacttcaatg gattagattt atagatttcg aaaaagaaag
      241 tgcacagaaa tataaaaaaa tatatgctcg atttttagtt gttataaatg tgttaactac
      301 tatcactgta gtatttgcac tgtgtaatct ttattatttt gcagctgtac aaaaccatta
      361 tgatttattt aattattggt tgatgggttc gatttcaatc ataattagct atttattatt
      421 agtaa
//
LOCUS       DAKTIP010000459          419 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.471, whole genome shotgun sequence.
ACCESSION   DAKTIP010000459 DAKTIP010000000
VERSION     DAKTIP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.471"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            46..>419
                     /locus_tag="O0305_002992"
     CDS             46..>419
                     /locus_tag="O0305_002992"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; lysine and threonine sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCY0276054.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNA"
BASE COUNT          154 a           51 c           74 g          140 t
ORIGIN      
        1 aactgaatac aattttctga ttttttagaa aggttggcat gcactatgaa agtttccaaa
       61 ttcggtggta gctccgtctc caatgctact caaattaaaa aggttttaaa tattgttaat
      121 tctgatccag agcgaaagat tgttatcgtt tctgctccag gtaaaagaca tgataatgat
      181 attaaaacaa ctgatttgtt aatcagatta tatgaaaagg tcattaatca tcttgattat
      241 catgataaaa aaagagaaat tattcagcgt tatgatgata ttgtaaaaga attgcaaatg
      301 gatgaaagta ttttacggac gatagatgtg actttggaac attatattaa tcaattaaaa
      361 aatgaaccaa agagactatt agatgcatta ctttcttgtg gtgaagattt taatgcgca
//
LOCUS       DAKTIP010000460          418 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.472, whole genome shotgun sequence.
ACCESSION   DAKTIP010000460 DAKTIP010000000
VERSION     DAKTIP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.472"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>418
                     /locus_tag="O0305_002993"
     CDS             <1..>418
                     /locus_tag="O0305_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCY0276055.1"
                     /translation="LGIVTVTLLTLVFLNRKISMKNRFLIMVTWNIDEPGGVIKLIKH
                     LAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSAFNNAGFALFKNNLIDYSS
                     DPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH"
BASE COUNT          133 a           50 c           67 g          168 t
ORIGIN      
        1 tctgggtatc gtgaccgtaa ccctattgac actagtattt ttaaatagaa agatatcaat
       61 gaaaaataga ttcttgatta tggttacatg gaatattgac gaacctggtg gtgttattaa
      121 gctaattaaa cacttggcta tttatagttt agtcactgaa ttaattggta tgatttgttt
      181 gtgtttatct tttataccaa aatttggtat aggcaaaggt ttatttttaa gcttattcac
      241 atcagtatca gcttttaata atgctggatt tgcccttttt aagaataact taatagatta
      301 ttctagtgat ccaattgtca ttattacaat ctcaatactc ataatatttg gaggtattgg
      361 acattttgtc gtgatagact ttattaattg taaaaaattg agtaaattat ctttacat
//
LOCUS       DAKTIP010000461          417 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.473, whole genome shotgun sequence.
ACCESSION   DAKTIP010000461 DAKTIP010000000
VERSION     DAKTIP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.473"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(30..>417)
                     /locus_tag="O0305_002994"
     CDS             complement(30..>417)
                     /locus_tag="O0305_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276056.1"
                     /translation="FKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFGDT
                     FLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLMLT
                     DFQKENYNKYIQGQKQRVIFENGHFV"
BASE COUNT          140 a           77 c           34 g          166 t
ORIGIN      
        1 cctcttactc taattatatt cactatcaat tagacaaaat ggccattttc aaatatcacg
       61 cgttgtttct gaccttgaat atatttatta taattctctt tttgaaaatc agttaacatt
      121 aatttagatg taccgtattt taacactttt tgcattgttt ctattctcat ttttctaaat
      181 aaccatccat cttttaacac aatacgatta acagcatcat atgataattc tactgtttct
      241 ttaatttcaa atgtcttgaa tgaaataatc gtgcacatta aaaacgtatc accaaagtaa
      301 taaacatcta aatcatcacg tttatgttgt ccaactaaca aacgaccata ttcgaactct
      361 ttttctggat atttcaattc taaaaaacta ataatctctt cttcttttaa tttgaat
//
LOCUS       DAKTIP010000462          413 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.474, whole genome shotgun sequence.
ACCESSION   DAKTIP010000462 DAKTIP010000000
VERSION     DAKTIP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.474"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            2..>413
                     /locus_tag="O0305_002995"
     CDS             2..>413
                     /locus_tag="O0305_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCY0276057.1"
                     /translation="MTAGCGIGKEAEVKKSFEKTLSMYPIKNLEDLYDKEGYRDDQFD
                     KNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTTNGYYYVDVTKDEDEGKPHDN
                     EKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFV"
BASE COUNT          177 a           48 c           81 g          107 t
ORIGIN      
        1 tatcactgct ggttgtggca taggtaaaga agcggaagtt aagaaaagct ttgaaaaaac
       61 attgagtatg taccctatta aaaatctaga ggatttatac gataaggaag gctatcgtga
      121 tgatcagttt gataaaaatg ataaaggtac atggattata aattctgaaa tggttattca
      181 acctaataat gaagatatgg tagctaaagg catggttcta tatatgaata gaaataccaa
      241 aacaacaaat ggttactact atgtcgatgt gactaaggac gaggatgaag gaaaaccgca
      301 cgacaatgaa aaaagatatc cggttaaaat ggtcgataat aaaatcattc caacaaaaga
      361 aattaaagat gaaaaaataa aaaaagaaat cgaaaacttt aagttctttg ttc
//
LOCUS       DAKTIP010000463          413 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.475, whole genome shotgun sequence.
ACCESSION   DAKTIP010000463 DAKTIP010000000
VERSION     DAKTIP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.475"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            157..>413
                     /locus_tag="O0305_002996"
     CDS             157..>413
                     /locus_tag="O0305_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCY0276058.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLL"
BASE COUNT          138 a           44 c           71 g          160 t
ORIGIN      
        1 tgatgactaa acaattttat aaattctgtg aagtatactt atatataaga taatcaactt
       61 caacgtatat caccatatta ttttatactc aatgatgtga atggatttaa gtggattgat
      121 attaaagtaa aagttatgga atattgaggt gtgaatatgg agttcttatt attaattgtc
      181 gtagccggac tgtattatat tatatattta actgctgtga tgtattctga aaaaatagta
      241 gtattgccta taatcatcta tgccattgtg tttgtaataa ttggtatcac ttatatcttt
      301 ataggcgaca gctatgatca attaacaaat ttcaatgtga ttttgtatat ggggagtttg
      361 ttttatgcat ggatggctat tagaaatctt tggaacagac cactattact aaa
//
LOCUS       DAKTIP010000464          409 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.476, whole genome shotgun sequence.
ACCESSION   DAKTIP010000464 DAKTIP010000000
VERSION     DAKTIP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.476"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>409
                     /locus_tag="O0305_002997"
     CDS             <1..>409
                     /locus_tag="O0305_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCY0276059.1"
                     /translation="KDFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSK
                     FENNRQTFDKIYNILNIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNA
                     LDKKTVNEIYEFLNDFIQSHQTKTIINITHNLSD"
BASE COUNT          175 a           58 c           41 g          135 t
ORIGIN      
        1 taaagatttc ttactaaata atagtggaaa tatccctaag tccatttctc aaaatgtaac
       61 cttaatatca agttcatcaa atattcctaa tgatataaca aaagattttt tattatcatt
      121 gttaaaatca aaatttgaaa acaatcgaca aacattcgat aagatttata acatactaaa
      181 catcgaagca ataccgtcta acgtactact aaaaaacttg agtgatggtc aaaaacaaaa
      241 gcttaaatta ttaagtttct tattggaaga tcatgattta atcatattag atgaagttac
      301 aaacgcttta gacaagaaaa cagttaatga aatttatgaa tttttaaatg attttattca
      361 aagccatcaa actaaaacta ttatcaatat tacacataat ttatccgat
//
LOCUS       DAKTIP010000465          406 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.477, whole genome shotgun sequence.
ACCESSION   DAKTIP010000465 DAKTIP010000000
VERSION     DAKTIP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.477"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>406)
                     /locus_tag="O0305_002998"
     CDS             complement(<1..>406)
                     /locus_tag="O0305_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMEK domain-containing protein"
                     /protein_id="HCY0276060.1"
                     /translation="IRLNTKIEELGKINILNNHIYSEYFFRDLLNIVYGYSLENHNKK
                     QKNAPAFDLIDNTNKIIIQVTATCKKQKIEDTLKKEYLTNKMEEGYRLKFIFIGNQNN
                     NIKNKNFSNPHNILFDSKKDIILTQDLCEEFLN"
BASE COUNT          130 a           51 c           39 g          186 t
ORIGIN      
        1 attcaaaaac tcttcgcata agtcttgagt taaaataata tcttttttac tatcgaataa
       61 tatattatgt ggatttgaaa aattcttatt tttaatatta ttattttgat ttccaataaa
      121 tataaacttt aatcgataac cttcttccat tttatttgtt aaatactctt tttttagagt
      181 gtcctctatt ttttgctttt tacacgttgc ggtaacttga ataataattt tattcgtatt
      241 atctataaga tcaaatgctg gtgcattttt ttgtttttta ttatgattct caagtgaata
      301 cccatataca atatttaaca ggtctctaaa aaaatattcg gaatatatat gattatttaa
      361 aatatttatc tttcctagtt cttcaatctt agtattaagt ctaatt
//
LOCUS       DAKTIP010000466          393 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.478, whole genome shotgun sequence.
ACCESSION   DAKTIP010000466 DAKTIP010000000
VERSION     DAKTIP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.478"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(38..>393)
                     /locus_tag="O0305_002999"
     rRNA            complement(38..>393)
                     /locus_tag="O0305_002999"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a          112 c           77 g          107 t
ORIGIN      
        1 gtaagtaaaa gtgattttgc ttcgcaaaac atttattttg attaagtctt cgatcgatta
       61 gtattcgtca gctccacatg tcaccatgct tccacctcga acctattaac ctcatcatct
      121 ttgagggatc ttataaccga agttgggaaa tctcatcttg aggggggctt catgcttaga
      181 tgctttcagc acttatcccg tccacacata gctacccagc tatgccgttg gcacgacaac
      241 tggtacacca gaggtatgtc catcccggtc ctctcgtact aaggacagct cctctcaaat
      301 ttcctacgcc cacgacggat agggaccgaa ctgtctcacg acgttctgaa cccagctcgc
      361 gtaccgcttt aatgggcgaa cagcccaacc ctt
//
LOCUS       DAKTIP010000467          383 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.479, whole genome shotgun sequence.
ACCESSION   DAKTIP010000467 DAKTIP010000000
VERSION     DAKTIP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.479"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>383
                     /locus_tag="O0305_003000"
     rRNA            <1..>383
                     /locus_tag="O0305_003000"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           80 c          116 g           79 t
ORIGIN      
        1 caaccgtgga gggtcattgg aaactggaaa acttgagtgc agaagaggaa agtggaattc
       61 catgtgtagc ggtgaaatgc gcagagatat ggaggaacac cagtggcgaa ggcgactttc
      121 tggtctgtaa ctgacgctga tgtgcgaaag cgtggggatc aaacaggatt agataccctg
      181 gtagtccacg ccgtaaacga tgagtgctaa gtgttagggg gtttccgccc cttagtgctg
      241 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga
      301 attgacgggg acccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa
      361 ccttaccaaa tcttgacatc ctt
//
LOCUS       DAKTIP010000468          382 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.480, whole genome shotgun sequence.
ACCESSION   DAKTIP010000468 DAKTIP010000000
VERSION     DAKTIP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.480"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>382
                     /locus_tag="O0305_003001"
     rRNA            <1..>382
                     /locus_tag="O0305_003001"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          107 a           84 c          112 g           79 t
ORIGIN      
        1 aaaagtgaaa gacggtcttg ctgtcactta tagatggatc cgcgctgcat tagctagttg
       61 gtaaggtaac ggcttaccaa ggcaacgatg catagccgac ctgagagggt gatcggccac
      121 actggaactg agacacggtc cagactccta cgggaggcag cagtagggaa tcttccgcaa
      181 tgggcgaaag cctgacggag caacgccgcg tgagtgatga aggtcttcgg atcgtaaaac
      241 tctgttatta gggaagaaca tatgtgtaag taactgtgca catcttgacg gtacctaatc
      301 agaaagccac ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttat
      361 ccggaattat tgggcgtaaa gc
//
LOCUS       DAKTIP010000469          363 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.482, whole genome shotgun sequence.
ACCESSION   DAKTIP010000469 DAKTIP010000000
VERSION     DAKTIP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.482"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            105..>363
                     /locus_tag="O0305_003002"
     CDS             105..>363
                     /locus_tag="O0305_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276061.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVI"
BASE COUNT          116 a           46 c           49 g          152 t
ORIGIN      
        1 taaaaatgac tttagcaaac attgtgtcta caagttaata aaactattgt tttatgattg
       61 tttatgatac gatatgtaaa tgataatcat ccaggaggtc tagcatgtct cgttcaaaaa
      121 aatactttta cttatctagc ttaatgatta ttttaagctt tttctttaat acaaataacg
      181 ttttcctaag tggacttttt aattctttta ttaaattaat acttttctgc agtgttatta
      241 actcaattgt actaattttg tctataattt ttgcagatcg ttcaattaaa tcactaaagc
      301 ctgatgcaga ttggattaga attgcgagta aaagtttgcc ttggattatt ctaattgtta
      361 ttt
//
LOCUS       DAKTIP010000470          354 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.484, whole genome shotgun sequence.
ACCESSION   DAKTIP010000470 DAKTIP010000000
VERSION     DAKTIP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.484"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..279)
                     /locus_tag="O0305_003003"
     CDS             complement(<1..279)
                     /locus_tag="O0305_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002434158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276062.1"
                     /translation="MENTNSNMHKNDNSYVLSALSYLSIFFAPVILPLFVWILADEPT
                     SEHGKKAFINHIMTWVSFFIGRLAFIFSKEVFDKPLDHQLLIFSILLII"
BASE COUNT          139 a           52 c           41 g          122 t
ORIGIN      
        1 aataattaaa agaatgctaa atattaataa ttgatgatcc aaaggtttat caaagacttc
       61 tttagaaaaa ataaatgcca atctacctat aaaaaaacta acccaagtca taatatgatt
      121 aatgaatgct tttttgccat gttctgatgt tggttcatca gcaagtatcc atacaaataa
      181 aggtaaaatg acaggtgcga aaaagatact taaataactc aatgctgata aaacgtaact
      241 attgtcattt ttatgcatat tactatttgt attttccact tttttcacct tctttacttt
      301 tctttactat tataaaaaca ttaatagcga gctattacaa cagttataag agaa
//
LOCUS       DAKTIP010000471          342 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.486, whole genome shotgun sequence.
ACCESSION   DAKTIP010000471 DAKTIP010000000
VERSION     DAKTIP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.486"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          132 a           45 c           50 g          115 t
ORIGIN      
        1 attaataatt ttacgtgctc gtcgtcaaga ctatttaaat caagtttagc catgtctgtt
       61 cctgtatagg cattaatttt taatactttc ttatttgaaa aaagcaaaaa agataggtga
      121 gatgcatacg caaccaaaag tgtataatat caatttacta cctcgcaata taattttata
      181 caatgtataa taagatttaa agatgtagct catcaacatt ttcatagggg atttttaaaa
      241 ttaagtattg aaaaataaaa tatttatatt ataatataaa taagtcaacg accatgcgtg
      301 gacagtaaaa cgaagtacat gcgaatgtag tagccgttta ct
//
LOCUS       DAKTIP010000472          328 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.487, whole genome shotgun sequence.
ACCESSION   DAKTIP010000472 DAKTIP010000000
VERSION     DAKTIP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.487"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            96..178
                     /locus_tag="O0305_003004"
     tRNA            96..178
                     /locus_tag="O0305_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Leu,seq:gag)
     gene            201..274
                     /locus_tag="O0305_003005"
     tRNA            201..274
                     /locus_tag="O0305_003005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Gly,seq:tcc)
BASE COUNT          109 a           59 c           68 g           92 t
ORIGIN      
        1 gtaaaaaaac gattaaaagt tattgacaaa tatttttaaa cataatataa taaataacgt
       61 tctgaaaaac gaagatgttt taaatgccta tatccgcggt cgtggcggaa tggcagacgc
      121 gctaggttga gggcctagtg ggagaaatcc cgtggaggtt caagtcctct cggccgcatc
      181 aaaattctta atttaaataa gcgggtgtag tttaatggca aaacctcagc cttccaagct
      241 gatgttgtgg gttcgattcc catcacccgc tccatagata attttaatga acattgaaaa
      301 ctgaatgaca atatgtcaac gttaattc
//
LOCUS       DAKTIP010000473          320 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.488, whole genome shotgun sequence.
ACCESSION   DAKTIP010000473 DAKTIP010000000
VERSION     DAKTIP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.488"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            163..>320
                     /locus_tag="O0305_003006"
     CDS             163..>320
                     /locus_tag="O0305_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCY0276063.1"
                     /translation="MFKNILLPYDFENDFSAIPDYLEKVTDEDSVVVIYHVVTENDLA
                     ISVKYYNK"
BASE COUNT          136 a           30 c           50 g          104 t
ORIGIN      
        1 aaataggttt atacaaagaa ttacaaaaat aatataaaaa atagaatcta atacatgata
       61 aggaatatat ttgaataatt tgaatatgag aataagcata catatttagg gtatatataa
      121 gtaatagaca tctaaataat aagtaatagg aggctggtgt agatgttcaa aaatatatta
      181 ttaccctatg atttcgaaaa tgattttagt gctatccctg actatttaga aaaagtcacc
      241 gatgaagatt cagttgttgt aatttatcac gttgtaacag aaaatgatct tgcaattagt
      301 gtcaagtatt ataataagca
//
LOCUS       DAKTIP010000474          318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.489, whole genome shotgun sequence.
ACCESSION   DAKTIP010000474 DAKTIP010000000
VERSION     DAKTIP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.489"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..213)
                     /locus_tag="O0305_003007"
     CDS             complement(<1..213)
                     /locus_tag="O0305_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCY0276064.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLE"
BASE COUNT          114 a           41 c           41 g          122 t
ORIGIN      
        1 ttctaaatag tatggtttat caatattttt cgcgaaataa ccagatataa acaaaaatgt
       61 tggcatatgg aaactataaa taactaggta taatgctgat aaatatttat cgccactagt
      121 gtagggttgt aacatatgtc caaatacgac taataatatt aaaattgctc ttgcgttgtc
      181 aaaaaaataa tctctctctt ttaatgaagt catattttat ctcctttatg tccataagac
      241 tcttactaat ttattatatt ttgtattagt tatgcaacaa atttgtctat tgtgtcgaat
      301 agtattgtaa aaattata
//
LOCUS       DAKTIP010000475          307 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.490, whole genome shotgun sequence.
ACCESSION   DAKTIP010000475 DAKTIP010000000
VERSION     DAKTIP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.490"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>307
                     /locus_tag="O0305_003008"
     CDS             <1..>307
                     /locus_tag="O0305_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCY0276065.1"
                     /translation="LFLPMINWLIPKKYVKISKKEFDDLNIVKPVKNKAFWPVAGSTI
                     LFGVTFRKYIPSLNIQLEKNMVIVICCAIFLGVLILFLFLNRKLRLEIYNNNSSKGKI
                     "
BASE COUNT          110 a           33 c           48 g          116 t
ORIGIN      
        1 ttatttcttc ctatgattaa ttggttaatt ccaaaaaaat atgtcaaaat cagtaaaaaa
       61 gaatttgacg atttaaacat tgtcaaacct gttaaaaata aagctttttg gccagttgca
      121 ggtagtacta ttttgttcgg agttacgttt agaaaatata ttccttcact taatattcaa
      181 ttagagaaaa acatggtgat tgtaatatgt tgtgcaatat ttctgggtgt tttaatactt
      241 tttttatttc tgaatcgtaa gctaaggttg gaaatttata ataataactc tagtaaaggg
      301 aaaataa
//
LOCUS       DAKTIP010000476          299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.492, whole genome shotgun sequence.
ACCESSION   DAKTIP010000476 DAKTIP010000000
VERSION     DAKTIP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.492"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
BASE COUNT          109 a           35 c           47 g          108 t
ORIGIN      
        1 tactctatag aaggaaaagt tatccactta tgcacattta tagttttcag aattgtggat
       61 aattagaaat tacacacaaa gttatactat ttttagcaac atattcacag gtatttgaca
      121 tatagaggac tgaaaaagta taattgtgtg gataagtcgt ccaactcatg attttataag
      181 gatttattta ttgattttta cataaaaata ctgtgcataa ctaataagca agataaagtt
      241 atccaccgat tgttattaac ttgtggataa ttattaacat ggtgtgttta gaagttatc
//
LOCUS       DAKTIP010000477          278 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.496, whole genome shotgun sequence.
ACCESSION   DAKTIP010000477 DAKTIP010000000
VERSION     DAKTIP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.496"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(24..>278)
                     /locus_tag="O0305_003009"
     rRNA            complement(24..>278)
                     /locus_tag="O0305_003009"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           69 a           76 c           63 g           70 t
ORIGIN      
        1 agatgttccg aatatatcct tagaaaggag gtgatccagc cgcaccttcc gatacggcta
       61 ccttgttacg acttcacccc aatcatttgt cccaccttcg acggctagct cctaaaaggt
      121 tactccaccg gcttcgggtg ttacaaactc tcgtggtgtg acgggcggtg tgtacaagac
      181 ccgggaacgt attcaccgta gcatgctgat ctacgattac tagcgattcc agcttcatgt
      241 agtcgagttg cagactacaa tccgaactga gaacaact
//
LOCUS       DAKTIP010000478          276 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.497, whole genome shotgun sequence.
ACCESSION   DAKTIP010000478 DAKTIP010000000
VERSION     DAKTIP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.497"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(24..128)
                     /locus_tag="O0305_003010"
     CDS             complement(24..128)
                     /locus_tag="O0305_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276066.1"
                     /translation="MLNIKAIKPSEKGIKVLSHQFKQVSKTQLNYFFN"
BASE COUNT           93 a           41 c           28 g          114 t
ORIGIN      
        1 attttttgct gttttcatcg tcattagtta aaaaaataat ttaactgagt ttttgagacc
       61 tgtttaaatt gatgactcaa aacctttatc cccttttcac tcggtttaat tgctttaata
      121 tttaacacag tctcattgta attttgctac taagtttgaa atatttcgat tcgaatgtga
      181 aacatcattt attattactt tcgacagcta gaaaaatatt gttaaaccaa aactataatt
      241 accactttta tatagaacta tatataaatt ttaact
//
LOCUS       DAKTIP010000479          265 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.498, whole genome shotgun sequence.
ACCESSION   DAKTIP010000479 DAKTIP010000000
VERSION     DAKTIP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.498"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..>265
                     /locus_tag="O0305_003011"
     CDS             <1..>265
                     /locus_tag="O0305_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276067.1"
                     /translation="ISFKLGIKYLLTIKRGNIEKDRFSISIIFKDTYHTLVRIDINGG
                     THDNPDGTIAPKSHIHIYNDKYDKKDRFAYEINLKDFPDIYNLY"
BASE COUNT          108 a           37 c           41 g           79 t
ORIGIN      
        1 ataagtttta agttaggaat aaagtactta ttaacaataa aaagagggaa catagaaaaa
       61 gataggtttt caatttcaat cattttcaaa gatacctatc acaccctagt tagaatagat
      121 attaacggtg gtactcacga taatccagat ggaacaatcg ctccgaaaag tcatattcac
      181 atatataatg ataagtatga taaaaaggat aggtttgctt atgaaattaa cttgaaagat
      241 ttccccgaca tctataactt gtata
//
LOCUS       DAKTIP010000480          262 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.denovo.499, whole genome shotgun sequence.
ACCESSION   DAKTIP010000480 DAKTIP010000000
VERSION     DAKTIP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.denovo.499"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            complement(<1..>262)
                     /locus_tag="O0305_003012"
     CDS             complement(<1..>262)
                     /locus_tag="O0305_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0276068.1"
                     /translation="KINDSNYRLSNVDTMKVTLYSNGSNYDKEALLINKDEFCPLRKI
                     TLDNKLDSQRVMEIDSLAAIINLVKQGKGKALLPMTFENKRDI"
BASE COUNT           79 a           47 c           26 g          110 t
ORIGIN      
        1 ctatatctct tttattctca aaagtcatag gtaataaagc ctttcctttt ccttgtttaa
       61 ctaagtttat tattgctgcc aatgaatcaa tttccattac ccgttgtgaa tcaagcttat
      121 tatctaaagt tattttcctt aaaggacaaa actcatcttt atttattagt aaagcctctt
      181 tatcataatt acttccatta ctgtaaagcg ttactttcat agtatcaaca ttactaagtc
      241 tataatttga gtcgttaatt tt
//
LOCUS       DAKTIP010000481         6868 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.137, whole genome shotgun sequence.
ACCESSION   DAKTIP010000481 DAKTIP010000000
VERSION     DAKTIP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.137"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..83
                     /locus_tag="O0305_003013"
     CDS             <1..83
                     /locus_tag="O0305_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCY0276069.1"
                     /translation="FVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(255..1226)
                     /locus_tag="O0305_003014"
     CDS             complement(255..1226)
                     /locus_tag="O0305_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCY0276070.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKD
                     MLALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEY
                     AQVPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGG
                     VGSVAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLD
                     TVGSNVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIR
                     EDIWSKLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
     gene            1350..1769
                     /locus_tag="O0305_003015"
     CDS             1350..1769
                     /locus_tag="O0305_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCY0276071.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLIN
                     SNLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWP
                     TLEKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
     gene            complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0305_003016"
     CDS             complement(2295..3140)
                     /gene="blaZ"
                     /locus_tag="O0305_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCY0276072.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALN
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            3247..5003
                     /gene="blaR1"
                     /locus_tag="O0305_003017"
                     /pseudo
     CDS             3247..5003
                     /gene="blaR1"
                     /locus_tag="O0305_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
     gene            4993..5373
                     /gene="blaI"
                     /locus_tag="O0305_003018"
     CDS             4993..5373
                     /gene="blaI"
                     /locus_tag="O0305_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCY0276073.1"
                     /translation="MTNKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            5637..6215
                     /locus_tag="O0305_003019"
     CDS             5637..6215
                     /locus_tag="O0305_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0276074.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG
                     LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNG
                     TPIKTIAEQWQVSRTTIYRYLNKLEAKENKNK"
     gene            complement(6438..6623)
                     /locus_tag="O0305_003020"
     CDS             complement(6438..6623)
                     /locus_tag="O0305_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276075.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTIL
                     ILLTSIITVVSVNDEDT"
BASE COUNT         2573 a          950 c          945 g         2400 t
ORIGIN      
        1 ttttcgttca catcatagca ccagtcatca gtggctgtgc tgttgcgtat tatacttatt
       61 ggcttagtaa acgcaacaaa taattaatag gcgactttat aagccagcat cgaaaaaatc
      121 cccacactac tgcaatagtg tggggattgg tgcattaatt gttaatcatt ttcgttatat
      181 aaattataac actggttagt agagaaatgc aaaatgattg aattaatagt aaatgtatta
      241 atgaatactc acacttatac ttttaaaata gttcttccta aatgttgtcc ttttaaaagc
      301 tgatctatcg ttttatagaa ttcttcaaat ccaatttcat tgtatagaag tgaatttcct
      361 atattccatt catttgctaa cttactccat atatcttcac ggatattatg atcaacatat
      421 acagaatcaa tgccaattaa tttaatacct ctaagaataa acggtaatat attagcgtta
      481 agtgaatttc ctgctacatt tccacataca gtagcaacac cttgataatt tagacgtttt
      541 aaaccgtata atgttacatt actccccact gtatctaaga aataatcaaa aatacctttt
      601 ttaagcggtt tattatcatc ttcaattatt attacttgtt ttgcacctaa tttttttgcg
      661 atttctattt gcttttcttt tcttacgata gcagtgatat tttcatatcc aattttatgt
      721 aaaatttgta tagcaacact gcccacacca cctgtagccc cagacactaa aatttcaggt
      781 tgtgatttga tattcattcc atatttttct agttcaaaaa ttgataatgc tgccgtaaaa
      841 ccggctgtgc catataccat agactcttta atagacattt cttggggaat aggaacaatc
      901 cactctccag gcacttgtgc atattctgaa aatccacctg tatgtgtcac gccaacatca
      961 taaccagtaa ctaatacatt atcgccttct ttaaacttat cattcgaact tgattcaaca
     1021 atacctgcaa aatctatacc aggaatcata ggataatctc taattacacc gcctttatat
     1081 tgtaaagcta gcatatcctt ataatttatt gaagagtata ccacttttac taatacatca
     1141 cccttcgata aatgagattc atcaactttt tctactttat aactaacatt tgatgcactt
     1201 tttctcacta ctaccgcatt gaacatttat tagccttctt tcacaattgt tttgtagaag
     1261 tatgtttttg ttgcaaaact attttcctac aaaacttatc atgatatgat aagcgagtca
     1321 atactaatga tttaagagag gttttagttg tggaattaga taattgtata aattatttgt
     1381 tgagtacttc tcagaataga gtttttaaac attttaaaag tttattaagt ccctataata
     1441 ttactccagc agaatatggt gttttgaatt gcttgattaa tagtaatttg aatacaccta
     1501 aagcgattgg taatacttta aatttagaac cttcaacaat atctggaata ttagataaga
     1561 tgtccaaaaa ggatctaata acacgtcaaa ctgataatga aaatagaaga actgtgctaa
     1621 taaacagtac aaataaagca aaagacttat ggccaacatt agaaaagaaa gcctatcaac
     1681 ttaataatca ttacttcgct aatttaacta atgaagaaac tgtagtgttt aaaaagatat
     1741 tactaaagat aaataaaact acgttttaat ttttacgtta taaaaaagtt gaagtgcgaa
     1801 ttataatcga gtaaatataa atgatagctt agcttaatta tatattaaag ccaactatac
     1861 ttttattccc gaaaaaccta ctatccaaat ttctaagata ctttagcctc tataataaac
     1921 atattgtaat ataatttatg tattatttag gtttacccta atcgaacaat aaaaaatact
     1981 tgtaaaatgc tattgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
     2041 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
     2101 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
     2161 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
     2221 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
     2281 atgcatttag aatattaaaa ttccttcatt acactcttgg cggtttcact tatcaactta
     2341 tcatttggct tatcactttt attgtcttta ttcgtaaaaa tgactaaaac aataggttca
     2401 gattggccct taggataaac aaaagcaaca tcatttctag aagcatatgt tattgcttga
     2461 ccacttttat cagcaacctt atagtctttt ggaacaccat ctttaattaa agtgtctccg
     2521 tttttattat taaacattaa atcaagtaag aaatttttat tttttttgct taattttcca
     2581 tttgcgataa gtttatttaa agtcttgccg aaagcagcag gcgttgaagt atcttttttg
     2641 ctctttggtg aatagtaatt taattctatc tcatatctaa ctggatttgt tactttatct
     2701 cctagttctt ttagacgttg tttaactttt ttgattccac cgatttcttt tataatttta
     2761 ttgtttgctg tattatcact atatgtcatt gaagcctcaa taagttcttt taaagtgata
     2821 tcttttccta catatttttc taaaatagga gaataagcaa ctatatcatc tttgttaata
     2881 tgtacttttt tatttaactt attataaggt acttgttcta acaaaatagc actatttatc
     2941 gcttttgaag tcgaagcata ggcaaatctc ttatctgaat taaattttac ttccttacca
     3001 cttttagtat ttaaagcata aacaccaata tgagcattat attttttttc taaatcattt
     3061 aactctttgg catgtgaact gtttgaatta catgcactta aaactaaagc aattacaatt
     3121 aaaaatatta actttttcaa aataaaccct ccgatattac agttgtaatt tttataaatc
     3181 aataatatta caattgtaat attgatgtca attgtttatt ataaatattt gaaagaaggt
     3241 gtcaaaatgg ctaaattact aataatgagc gtagtaagct tttgttttat atttctattg
     3301 ttagtatttt ttaggtacat attaaaacgc tattttaatt atagtttaaa ttataaagtt
     3361 tggtatctaa ctatgcttgc tggattaatt ccttttatcc caattaaatt ctcttttata
     3421 aaatttaata atgtgaataa tcaagcgccc acagttgaaa gtaagtcaca cgatttgaac
     3481 cataacataa ataccaccaa acctattcaa gagttcacaa cagatatcca taagtttaat
     3541 tgggattcaa ttgataatat ctgcacagtt atttggatag ttttagttat tattttaagt
     3601 tttaaatttt tgaaagcctt attatatctt aaatatttaa agaaacagtc actttatcta
     3661 aacgaaaatg aaaaaaataa aatagatacg atacttttca accatcaata taaaaaaata
     3721 ttgtgattcg aaaagcagag gctattcaat ctccaataac tttttggtat gggaaatata
     3781 ttattttgat tcctagttca tattttaaaa gtgtaattga taaaagacta aaatatataa
     3841 tactgcatga atatgctcat gctaaaaata gagatacttt acatttaatt atctttaata
     3901 tcttctctat tattatgagt tacaatccat tagtacatat tgtaaaaaga aagataattc
     3961 atgacaatga agtagaagcc gatagatttg tccttaataa tattaacaaa aatgaattta
     4021 aaacttatgc ggaatctatt atggactccg tattaaaaac cccattttcc aacaaaaata
     4081 tattaagcca ttcatttaat ggtaaaaaat cattactcaa aagtagatta attaatataa
     4141 aagaagccga tctgaaaaag cagtcaaaat taattctaat ttttatttgt atttttactt
     4201 tttttatcat gataattcaa agccagtttt taatggggca atccttaact gattataatt
     4261 ataaaaagcc cttacaaagc gattaccaaa ttttagatga aagtaaaaat tttggttcaa
     4321 atagtggatc tttcgttatg tacagcatga agaaagacaa atactatatt tataatgaaa
     4381 aagaaagtag aaaaagatat tctcctgatt caacttacaa aatttattta gcactgtttg
     4441 gacttgaccg tcatattata agtgataaaa attcacgtat gtcatggaat cataaacatt
     4501 atcttttcga gtcttggaat aaggaacaag atttaaatac agcaatgcaa aattcagtta
     4561 attggtactt cgaacgtatt agtaatcaaa taccaaagaa ctatactgcg gctcaactca
     4621 agcaattaaa ttatggtaat gaaaatttgg gaagttataa aagctattgg atggaagata
     4681 gtttgaaaat atctaatctt gaacaagtaa tagtttttaa aaatatgatg gaacaaaata
     4741 accattttag taaaaaagca aagaatcaat tatcttcttc attattgatt aagaaaaatg
     4801 aaaagtatga actgtatggg aaaacaggta caggtatagt aaacgggaag tataataatg
     4861 ggtggtttgt aggttacgta attacaaatc atgataagta ttattttgct acacatttat
     4921 cagatggaaa cccgtctggg aaaaatgctg aattaataag tgaaaaaata ttaaaaggaa
     4981 tgggtgtttt aaatgaccaa taagcaagtt gaaatatcta tggctgaatg ggatgttatg
     5041 aatataatat gggataaaaa atcagtatca gctaatgaaa ttgtagttga aattcaaaaa
     5101 tataaagaag ttagtgataa aacgattaga acattaatca caagactata taaaaaagag
     5161 attataaaac gatacaaatc agaaaatatt tatttttact catcaaatat taaagaagac
     5221 gatattaaaa tgaaaactgc taaaaccttt cttaataaac tgtatggagg ggatatgaaa
     5281 agtttagtgc tgaattttgc gaaaaatgaa gaattaaata acaaagaaat tgaagaattg
     5341 cgagacattt taaatgatat tagtaaaaag taattagagg tatgtttcaa acataataca
     5401 tatgtaaacc atacttgact cttattaaat tagttttgtt aatgacattt aaaatatatt
     5461 attaaagatg agagtgtcca gaaaataacc attttctgta cactcttgtt ttttgtctaa
     5521 ataagtaagt attacataga attatgtgtt caaaaaccca acaaaaaatc tatgttcaca
     5581 aaagacctcg taatcaagtt atggtacact aaaggtgtat ataggaggtc tgtattttga
     5641 aaataggtta tgcaagagtt tcaactggat tacaaaattt gaatttacag gaggatcgat
     5701 taaatacata tggttgtgaa aagatattta atgaccatat gagtggttca aaaagtaagc
     5761 gacctggttt agataaagca attgaatttg cgaggtcagg agatacgatt gttgtttgga
     5821 gattagatag actaggacgg aacatggagg atttaatcac gttagttaat gaacttaacg
     5881 aacgaggtgt gagttttcat agtttagaag aaaatataac gatggataaa tcaagttcta
     5941 caggacaatt attatttcat ttattcgcag catttgcaga gtttgaacgt aatttaattt
     6001 tagagcgatc atcagccggt cgaattgcag cacgtgctag aggccgatat ggaggtagac
     6061 ctgaaaaatt aaataaacaa gatttaaact tacttaagac actttatgat aatggtacgc
     6121 caattaaaac aattgcagag cagtggcaag tttcacgtac aactatttat cgttatctca
     6181 ataaattaga ggcaaaagag aataaaaaca agtagagcaa ctaatctagc ttactataat
     6241 gaatctacta aataatttta tatatcatta acatacaaag actttgattc aatgtcactc
     6301 gatgtaaaag agacgttctg acagcgtcta aaatctcata atattttcct gttatgagat
     6361 tttaataata acacaagaaa aaagcgtaca aattcgtacg cttttttaat ataatacctg
     6421 tttcactaat gaacgtttta agtatcttca tcattaactg aaactacagt aataatactc
     6481 gtcaacaata ttagtatagt taaaatagaa agacttagga ctggcataac cgaactaaaa
     6541 ttatatttga aatataaaag tgctaatgaa gagagaaatg taaaaataat aaataccaaa
     6601 gaccattttt ctaatttatt cattggcttc acctctttga acttttatat aatcatagga
     6661 gatattttta ctacgattta taaaaaataa gaagataatc attattaaag aaacgattaa
     6721 tataatgttg aatcctatag tatcacctaa ttcgtttaca cctttgccta atatcggaga
     6781 tacaaagctt tgtattaata atgaaagaga tgcccatata gtaaatgagg ttctgttgca
     6841 aagttgaatt tatagtataa ttttaaca
//
LOCUS       DAKTIP010000482         6633 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.143, whole genome shotgun sequence.
ACCESSION   DAKTIP010000482 DAKTIP010000000
VERSION     DAKTIP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.143"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            458..1924
                     /gene="msr(A)"
                     /locus_tag="O0305_003021"
     CDS             458..1924
                     /gene="msr(A)"
                     /locus_tag="O0305_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCY0276076.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIGHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETVGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLKNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRQYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALDRSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSLHDIT"
     gene            2023..2922
                     /locus_tag="O0305_003022"
     CDS             2023..2922
                     /locus_tag="O0305_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="HCY0276077.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVE
                     WVLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATID
                     PEIQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRR
                     MNKVKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDW
                     TEATHSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIA
                     EFAMESGESAYETMALKELGMKE"
     gene            3408..4016
                     /locus_tag="O0305_003023"
     CDS             3408..4016
                     /locus_tag="O0305_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCY0276078.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRP
                     VFQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGN
                     PLLDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQ
                     KRVIYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
     gene            4262..4600
                     /locus_tag="O0305_003024"
     CDS             4262..4600
                     /locus_tag="O0305_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276079.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRK
                     MFIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYY
                     TTNKKYTSKG"
     gene            4605..4790
                     /locus_tag="O0305_003025"
     CDS             4605..4790
                     /locus_tag="O0305_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCY0276080.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTL
                     AFKLARLFDTTVDELFS"
     gene            complement(5017..5760)
                     /locus_tag="O0305_003026"
     CDS             complement(5017..5760)
                     /locus_tag="O0305_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCY0276081.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI
                     EKIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEK
                     QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST
                     NTRQNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPA
                     NLNDNDSDIEEKSEETEPKKGFWSRLFGS"
     gene            6186..>6633
                     /locus_tag="O0305_003027"
     CDS             6186..>6633
                     /locus_tag="O0305_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCY0276082.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILR
                     DRLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGL
                     NKPNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFENR"
BASE COUNT         2523 a          946 c         1023 g         2141 t
ORIGIN      
        1 tgttaaaatt atattataaa ttcaactttg caacagaacc gtattttatg agtacactct
       61 aaattaagga gatgtataat caatctaaaa ttgtattttt atacttttgt tatataatct
      121 ttctacttag agatatattg taaaaaaacc aacaaaatgt tatattaaca ttactatttt
      181 atcgatgaag gaggattaaa atgacagctt ctatgagact caaataaacc tttttgaata
      241 caaaactaaa ttaattcaaa aaggcttgtc cgcaatacac actcatagga tgacttatgt
      301 cattttttat ccttcgatag gaataataaa cgctttcaaa tactaaaaca tcataataaa
      361 tttttaagtg tttgaaagtg taatgtgaat gagacgggag tagcctgtct catttttttg
      421 cgtacacgtc ctttaaatat acaaggagtg tataaatatg gaacaatata caattaaatt
      481 taaccaaatc aatcataaat tgacagattt acgatcactt aacatcggtc atctttacgc
      541 ttaccaattt gaaaaaatag cacttattgg gggtaatggt actggcaaaa ccacattact
      601 aaatatgatt gctcaaaaaa caaaaccaga atctggaaca gttgaaacgg ttggcgaaat
      661 tcaatatttt gaacagctta acatggatgt ggaaaatgat tttaacacgt tagacggtag
      721 tttaatgagt gaactccata tacctatgca tacaaccgac agtatgagtg gtggtgaaaa
      781 agcaaaatat aaattagcta atgtcatatc aaattatagt ccgatattac ttttagatga
      841 acctacaaat cacttggata aaattggtaa agattatctg aaaaatattt taaaatatta
      901 ctatggtact ttaattatag taagtcatga tagagcactt atagaccaaa ttgctgacac
      961 aatttgggat atacaagaag atggcacaat aagagtgttt aaaggtaatt acacacagta
     1021 tcaaaatcaa tatgaacaag aacagttaga acaacaacgt caatatgaac agtatataag
     1081 tgaaaaacaa agattgtccc aagccagtaa agctaaacga aatcaagcgc aacaaatggc
     1141 acaagcatca tcaaaacaaa aaaacaaaag tatagcacca gatcgtttaa gtgcatcaaa
     1201 acaaaaaggc acggttgaga aggctgctca aaaacaagct aagcatattg aaaaaagaat
     1261 ggaacatttg gaagaagttg aaaaaccaca aagttatcat gaattcaatt tcccacaaaa
     1321 taaaatttat gatatccata ataattatcc aatcattgca caaaatctaa cattggttaa
     1381 aggaagtcaa aaactgctaa cacaagtacg attccaaata ccatatggca aaaatatagc
     1441 gctcgtaggt gcaaatggtg taggtaagac aactttactt gaagctattt accaccaaat
     1501 agagggaatt gattgttctc ctaaagtgca aatggcatac tatcgtcaac ttgcttatga
     1561 agacatgcgt gacgtttcat tattgcaata tttaatggat gaaacggatt catcagaatc
     1621 attcagtaga gctattttaa ataacttggg tttaaatgaa gcacttgatc gttcttgtaa
     1681 tgttttgagt ggtggggaaa gaacgaaatt atcgttagca gtattatttt caacgaaagc
     1741 gaatatgtta attttggatg aaccaactaa ttttttagat attaaaacat tagaagcatt
     1801 agaaatgttt atgaataaat atcctggaat cattttgttt acatcacatg atacaaggtt
     1861 tgttaaacat gtatcagata aaaaatggga attaacagga caatctcttc atgatataac
     1921 ttaaaaacta tacactaatt tatattgaat attaggttca tttatatcaa tataaaaaat
     1981 acgttcacaa gaaaaatata atagaaataa ggatccaata aaatgactcg acataatgaa
     2041 attattaaat gtgcagaaaa atatcaatta cacatccaac ctcaaacaat ctcattgaat
     2101 gaatcgggac ttgattttca agttgcattt ggaaaagata aacatggagt agaatgggtt
     2161 ttgagactac caagaagacc tgacgtttat aaacgaacaa aacccgaaaa acaaacggta
     2221 gacttcttac agaagaatgt ttcatttgaa ataccgaagt ggaaagtaca tgcaaaagac
     2281 cttattgctt acccaaaact tacaggtaaa cccgcagcca caatagatcc agaaatacaa
     2341 aattatgtat gggaaattga acacaaacca ttaccagaaa actttattaa cacattagct
     2401 gaaacactcg tagatttaca caacatacca gaagaaaaca ttaacgttca gcatataaat
     2461 atcaaaacca tacaagaaat aaaaaatgac tttcaaagaa gaatgaataa agttaaagaa
     2521 acttatggcg tatcagatga attatggaac agatggaaac aatggttaga aaacgacgaa
     2581 ctatggcctc gacatgcgac catgatacat ggggacttac atccaggaca tataatggta
     2641 gataaccaag caaacgtcac aggtctcata gactggactg aagcaaccca ctccgaccca
     2701 tcaatggact ttataggaca ccatcgtgta ttcgacgacg aaggattaga gcaacttata
     2761 acagcatacg gtaaagctgg aggtgaaata tggccacgaa tgaaagagca tataatagaa
     2821 ctcaatgcag tattcccaat gtttatcgct gagtttgcta tggaatcagg agaatcggcg
     2881 tatgaaacga tggcattgaa agagttaggt atgaaagagt agaacttata ttaaaagagg
     2941 cccattttaa agacgaaggc aatcctgatg aaattttaaa actgcgttta attagtgatg
     3001 gtctttggtt ttctataatg tattaaatat ctggacatgg aacaaatgca acaaatggaa
     3061 agaatcgttt tcgaaatttc taactcctta aatcaggagg ataaataaac ggtcattttc
     3121 attgaaagca cttatataaa tttcatcaat tttaatacct accgtaaata catttcataa
     3181 gtattaaaca gatgattaaa atttttagtt aatttaaata tttatgatag agatgacctt
     3241 caaatgaacg gttctgttac aaagaaaaaa tctatattta tttttagtaa gttttaatta
     3301 tagatatgta cactttgagt acaccctaac catacaaaaa gaattgttga aattataaca
     3361 attaaaacgt atgattaggg tgtatattta ttatggagtg aagatgaatg attataggtt
     3421 atgcgagagt atcatcgata gatcaaaatt tagaaagaca attggataat ttaaagacgt
     3481 ttggtgtgga gaaaatcttt acagagaaac agtcgggaaa gtcagtggaa aatagacctg
     3541 tatttcaaga agcactcaat tttgtcagaa tgggagatcg attcgttgtg gaatctattg
     3601 atcgtttagg acgtaactat gatgaaatta ttgaaactgt gaattattta aaagaaaaag
     3661 acgttcagtt aatgattacc agcttaccta tgatgaatga agtaattggc aatccattac
     3721 tggataagtt tatgaaagat ttaatcattc aaatattagc aatggtttca gaacaagaga
     3781 gaaatgaaag taaacgtcga caagcgcaag ggattcaagt tgcgaaggaa aaaggcgtat
     3841 ataaagggcg ccctttactt tattcaccaa atgctaaaga cccacagaaa cgtgttattt
     3901 atcatcgcgt ggtggaaatg ttagaagaag gcaaagcgat tagtaagatt gcgaaagaag
     3961 ttaatatcac aagacaaaca gtttatagaa ttaaacatga taagggatta tcttgataaa
     4021 ttgattgtca atgcaaatga gcgtctctca cagtgtctca aattccataa catattattg
     4081 aattgagatt ttaataatta tacaagaaaa aagagtacga gttcgcagct ttctatatag
     4141 taattgtttt actaataagg tgttttaaaa tatattataa tgtaaaaagt atttgacatt
     4201 tgtatcaaaa atatttacac tcataaatgt caaatacttt ttacatttat gaggtgttat
     4261 aatgaattta attcctttta ttattatttt aattttttct tgtttatgga cggctataac
     4321 taacaaatat ttaccttcag aaacattaga taaaaaagga caacaagatc gatacgatga
     4381 aagacaacgt aaaatgttta ttgaaatact ggctaaaagt tttatttgga ttgtatattg
     4441 tatgttattt acactagtgc ttaaatttac aggtttgtca ccaagtattg aaggatcttg
     4501 gttttctcaa tatccagaaa ttttctttat aattgtagca ctatttctta tgctgtttaa
     4561 ttactatact acaaataaaa aatatacatc taaaggttaa attaatgaaa aatagaatta
     4621 gagaatatag aaaacaaaaa ggaatttctc aagaaacttt aagtaaagaa ttaaaagtgt
     4681 ctaggcaaac tattaatgca atagaaaaca ataaatatga tcctacattg acattagcat
     4741 ttaagttggc aagattgttt gatacaactg tggatgaatt attttcttaa ccattcattt
     4801 atcacaaaaa gagagtgcga attcgcactc tctttttgtg tagtagtact attaaatttt
     4861 gtctcaataa cgaacgttta taacgcaatc atgatgagag tattaataca gttaattatt
     4921 tgaaatactt tagtttacat aatcgatgat taccagaagt tctcactata taaaagacac
     4981 taaaatttta taacttttag tgcctaatca attattttag ctaccaaaca aacgactcca
     5041 aaaccccttt ttaggttccg tttcttcact cttttcttct atatcactat cattgtcatt
     5101 tagattagct ggaacgtctt ctttttttgc ttcttcacct tgtgtacttt cgtcgctttg
     5161 acttccagat atatccttat attgaatctc tgtttcttta gtttcttcct gatctctgtt
     5221 aatatttaca ggattagtag tatatgtttt ttcttgtgca tcaacgtttt gtctagtgtt
     5281 agtagatgta tcaaagggat aattaaggtg tttttctttt tctaattgat tttctaattt
     5341 ttgaatcttt ttattactct ctaatgccag aacttgctga ttttcaagca attttgatct
     5401 ttcttgcaac tcttttctgt aaaattcaat tgttgtttca tgacgacttt cttgtttttc
     5461 aatttgatta tttaaagtat caatctgatt tttaaggatt tctactattt gagcatcgct
     5521 tttcgattca aaattaatat tatccgaccc ttttttttgt ttgtcttcag tagtactttc
     5581 aaaaccatat ttctttttat tttcatttac tctttgaatg attttttcta tatcggtatc
     5641 ttctttaata aacgaagtgt tttcctgttt tattgtctct atattcaatc gcttaatatt
     5701 attaaaaatc gtttgcttac taacccctaa ttcttcactc aactttttca cacttttcat
     5761 atcatcaacc ctttataaaa tgtttataaa cccttgataa aatattataa gcttataatg
     5821 cttaataata aagtgattaa taaataaaaa taaacatcca tcaacctttt tatcaagggt
     5881 ttatgaaagg tttatgatat ttatcaaggg tttataaacc cttgataaag ctatattttc
     5941 ccttttataa gtttatttaa agtgctataa taaacttaat ataacaaaaa ccccaactac
     6001 tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat ataattttcg
     6061 atacttgtat tatataatga ataatatctt agtgcaagca cctaaatcat taaataaatg
     6121 taaagctaaa taaaaacgtt ttattacctc ttaccaaact aaaatatgga aagaggtttt
     6181 ttgtcatgca aaatcaatat tttacagtgc aagaaaatta caaagaaaga ttttatcaaa
     6241 tccctaaagt attctttacc agtgaaaatt ataaaaatct aactaatgat atgaaaatag
     6301 cctatgctat cttacgtgat cgcttaaatt tatctattaa aaatagctgg gttgatgaag
     6361 atggtaacat ttactttgtt tattcaaatg aaaagctaat ggaaatctta aattgtaaaa
     6421 aagaaaagtt aacaaaaata aaaaaagggt tagaaaacga tggtctactc attcaaaaac
     6481 gacgtggttt aaataaacca aatattctat atttaatgaa acctattgtc acagaacgtg
     6541 atatttacaa aatagaaaaa gaagaaaacg atgttgaacc ttacggcgag aaggaagttc
     6601 gaaaatcgaa cgtccagaag ttcgaaaatc gaa
//
LOCUS       DAKTIP010000483         5318 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.176, whole genome shotgun sequence.
ACCESSION   DAKTIP010000483 DAKTIP010000000
VERSION     DAKTIP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.176"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..254
                     /locus_tag="O0305_003028"
     CDS             <1..254
                     /locus_tag="O0305_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCY0276083.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..505)
                     /locus_tag="O0305_003029"
     CDS             complement(245..505)
                     /locus_tag="O0305_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCY0276084.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(477..1463)
                     /locus_tag="O0305_003030"
     CDS             complement(477..1463)
                     /locus_tag="O0305_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCY0276085.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="O0305_003031"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="O0305_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCY0276086.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..4043)
                     /locus_tag="O0305_003032"
     CDS             complement(3615..4043)
                     /locus_tag="O0305_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCY0276087.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(4140..4883)
                     /locus_tag="O0305_003033"
     CDS             complement(4140..4883)
                     /locus_tag="O0305_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCY0276088.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1871 a          858 c          785 g         1804 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata atttaaatga tattgaaagt gtatgcatgc cagatgcaat gataccttta
     3901 aatctacttt gttctgcttt ttctttatct atatgcatat attgaggatc aaaagttgtt
     3961 gcaaattgga taatttcttc ttctgtaata tgaaggcttt ttgttttgaa tgtttctcct
     4021 actataaaat catcgtattt catatatgtc tctctttctt attcaaatta attttttagt
     4081 atgtaacatg ttaaaggtaa gtctaccgtc actgaaacgt aagactcacc tctaactttc
     4141 tattgagaca aatgcaccat tttatctgca ttgtctgtaa agataccatc aactccccaa
     4201 ttagcaagtt ggtttgcacg tgctggtttg tttacagtcc atacgttcaa ttcataaccc
     4261 gcttctttta ccatttttac ttttgcttta gtaagtttgg catcttcagt gtttactatt
     4321 ttagcattac agtaatctaa aagtgttctc cagtcttcac gaaacgaagt tgtatggaat
     4381 ataactgctc tgttatattg tggcatgatt tcttctgcaa gtttaacaag cacaacatta
     4441 aagcttgaaa tgagcacttc ttgattctga tttaagtttg ttaattgttc ttccacttgc
     4501 ttaaccatac ttttagaaag tgctagtcca ttcggtccag taataccttt taattctaca
     4561 tttaaattca tattatattc atttgctatt tttactacat catcgaaagt tggcaaatgt
     4621 tcatctttga atttttcacc aaaccaagat cctgcagaag catctttaat ttcatcataa
     4681 ttcaattcag ttatttcccc ggacatattt gtagtccgtt ctaaataatc atcatgaatg
     4741 ataatcagtt gttcatcttt tgtaattgca acatctaact ccaaccagtt tataccttct
     4801 acttctgaag cagctttaaa tgatgcaatt gtattttccg gagctttact aggtaatcct
     4861 ctatgtccat atacagttag catattacct ctccttgcat ttttattttt ttaattaacg
     4921 taactgtatt atcacattaa tcgcactttt atttccatta aaaagagatg aatatcataa
     4981 ataaagaagt cgatagattc gtattgatta tggagttaat ctacgtctca tctcattttt
     5041 aaaaaatcat ttatgtccca agctccattt tgtaatcaag tctagttttt ctgtacccct
     5101 tatctgcaat tttacttagg attgctttta acttacccct tatcagcaat tttactgaga
     5161 actgctttta acgcacctct tatctgcaat tttgcctaga actgctttta acgtacctct
     5221 tatctgcaat tttactgaga actgctttta acttacccct tatcagcaat tttgcatgga
     5281 attgctttta acgtacctct tatctgcaat tttactta
//
LOCUS       DAKTIP010000484         3299 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.233, whole genome shotgun sequence.
ACCESSION   DAKTIP010000484 DAKTIP010000000
VERSION     DAKTIP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.233"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            39..416
                     /locus_tag="O0305_003034"
                     /pseudo
     CDS             39..416
                     /locus_tag="O0305_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            413..1294
                     /locus_tag="O0305_003035"
                     /pseudo
     CDS             413..1294
                     /locus_tag="O0305_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1410..2339
                     /locus_tag="O0305_003036"
     CDS             1410..2339
                     /locus_tag="O0305_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCY0276089.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGA
                     GKTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLT
                     INAKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILD
                     EPTNGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEI
                     MYEDLKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINR
                     LLVLNNIDVFQMRVIEDNLETYFEDLVGGGTIG"
     gene            2332..3069
                     /locus_tag="O0305_003037"
     CDS             2332..3069
                     /locus_tag="O0305_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCY0276090.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKV
                     PTPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFL
                     VSKIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSP
                     PIIILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFL
                     LSLIIIIVTSIFSVIILFSYFKKTDIS"
     gene            3083..3286
                     /locus_tag="O0305_003038"
     CDS             3083..3286
                     /locus_tag="O0305_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCY0276091.1"
                     /translation="MGFIEQIKAFILGVVEGLTEFAPVSSTGHMIIVDDMWLNTADDL
                     GSASATTFKVVIQLGGSVAKLNL"
BASE COUNT         1201 a          433 c          587 g         1078 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca gccatttaat atgactgtta
       61 caattactaa gattcaagca ctattacgcc gcacgtatga tttcaccact caagttaata
      121 atttaacagt agatgattgc gagttactac ttgatgcagc aaagcttaaa tttaatgaag
      181 aaaccataga cctgacacac actgaattgc aaatcatgca tagcttattc cagaacaagg
      241 gcaaattcgt cagtcgtaat gcgttaatcg aaaaatgctg ggaatcagaa cacttcattg
      301 atgacaatac gcttgctgtc aacattacac gtttacgcaa aaagctacaa aaactaggac
      361 ttacagattt tatcgaaacg aagaaaaatg tcggatatcg tgtaggtgac gtatgaattt
      421 cttgaaatca atccaacgtg aaatttatat tgtcatcctt acttgtgctt tattctactt
      481 tatattattc atgtataatg tgccgatgca agcatatatg attggtctag gtattgtatt
      541 gtttgtttca atgatttact ggggtatcca ttacatgaac tttaaaaaag aataaacact
      601 acaggaacag aacgaagatt taagaatcga aaaccataaa cttcgtaatg actttattaa
      661 ctaccaaagt gagttagagt catatttttt aatgtgggtg catcaaatca agacaccgat
      721 tacagcatca aagttactgc ttgaagatcc tgatgaacac gtgatttcta acgtgcgtaa
      781 tgaagtctta caaattgata attacacgaa tcttgcactg aactatttga aactcatgaa
      841 ccaaaagaca tacatggtct ttatggaagt gagtgtagat gaattaattc gaccactgat
      901 taaacgttat gcgatacagt ttattcatca tcaaacaaag ttgcattatg agaaaagtga
      961 agcggttata ttaacagatg ccaaatgggc atcaattatg attgagcaaa tattaaacaa
     1021 tgcattaaaa tatgcacgtg gcaaagatgt gtggattacg tttgacgaaa atacaaagaa
     1081 attatcaatc cgagataacg gtatcggcat taatgcgagt gatttaccga aaatctttga
     1141 taaaggtttc tcgggctata acggtagaat aagtgagaaa tcaagcggta ttggtttata
     1201 tattgtcagc acaatctcaa agcgcttagg ccataaagta agtgctacat ctacattaaa
     1261 cgatggttca atattcacca ttcaatttaa ataactccaa tctttcattt ttgtaagatt
     1321 agcggctgtt tttgtaagaa tagataaagg cagccttttc taatcgcgcg taagataaag
     1381 gtaacaacaa actaagaaag gtgccagagg tgaaaaattt gaataatata attgaaataa
     1441 ataacttaac aaagcaatat ggaagtcaga tagcagttaa taatttgagt ataactgttg
     1501 aaagtggaca aatttatggt tttttaggaa gaaatggtgc aggcaaaaca actactataa
     1561 gaatgctttt aggcttaatt aaacctacga aaggtaatat taaaatattt gaaaaagagt
     1621 tcgaaagtaa taaaactgaa attttaaaaa atattggtgc tatggttgaa acgccaggtt
     1681 tttatagtaa tttgacagct aaagagaact taactataaa tgctaaaata ttaggtatac
     1741 aaaaagaaaa tgcaattgaa gaagtgttag aaatagtagg actagcttct gaaaaagtaa
     1801 aattgtttaa aaattattct ttaggtatga agcaacgttt agggattgcg agagctttat
     1861 tgcacaatcc cgaattattg atacttgatg agccgacgaa tggattagat cctattggca
     1921 taaaagaaat tagaaagttg attaaggatc tggctcaatc tagaaatata accattttaa
     1981 tttcaagtca tatattgtca gaaatagagc agttagtaga caaggtgggt attattcata
     2041 acggagtttt gttagaagaa attatgtatg aagatttaaa aaaaaagaac agacaattta
     2101 taagtatatc agtatcagat gaagaaaaag ttgcatgttt gttggaaact aacttcggaa
     2161 tttatgacta taaaattaaa gaaaatggcg aatttaaaat ttattcacat attgatagac
     2221 aacaagaaat taatagatta ttagtattaa acaatattga tgttttccag atgagagtga
     2281 tagaagataa tctagaaact tatttcgaag acttagtagg aggtggcaca attggataat
     2341 ctactccgta cagaatttat taagttaaaa agatcatata tgtttctaat aagtatttta
     2401 ggtacatgcg tagcaccttt tatggtggtt gtagcttctt ttattagtat gcggacgaaa
     2461 gtaccgactc ctaatattgt atttaaacag ttatttgaag aagtcagttt atatcatatg
     2521 ctaattattg gacctcctct atatggggtt ataatagcct atttgtttgg aagagaatat
     2581 atagaagata ctttaaaaaa cttattaatt atacctatat ctagagaaag ttttttagta
     2641 agtaaaataa tattattatt cttgtggatt caaacactat ctgttattgc atggttgcta
     2701 actttaatgc taggtatgct attgcatttt caagggttaa gccagagttt aataattgat
     2761 tcattcttta catttgttat tggcggagtg ttattatgga tattatctcc accaataatt
     2821 attttgtcga tatattttaa aaattatgtt cccacaattg ttttaaccat aataattact
     2881 ttgataaatt taatgactgc taattctgaa catagaggat tatttccttg ggctgcagtt
     2941 ggagacatat ctagaggaac attacctaat acttatccat ttctattatc tttaattatc
     3001 ataattgtta catctatatt cagtgtaatt atattattct catactttaa aaaaactgat
     3061 ataagttaaa aggaggagaa aaatgggttt tatagagcag attaaagcat ttattttagg
     3121 tgtagttgaa gggttgacag aatttgctcc agtatcatca acaggtcata tgattattgt
     3181 tgatgatatg tggttaaaca ctgctgacga tttaggaagt gcgtctgcaa ctacttttaa
     3241 ggtggtaata cagttaggag gttctgttgc aaagttgaat ttataatata attttaaca
//
LOCUS       DAKTIP010000485         3226 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.236, whole genome shotgun sequence.
ACCESSION   DAKTIP010000485 DAKTIP010000000
VERSION     DAKTIP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.236"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            122..757
                     /locus_tag="O0305_003039"
                     /pseudo
     CDS             122..757
                     /locus_tag="O0305_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            754..1284
                     /gene="sat4"
                     /locus_tag="O0305_003040"
     CDS             754..1284
                     /gene="sat4"
                     /locus_tag="O0305_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HCY0276092.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFEKAVFWYLRF"
     gene            1377..2171
                     /locus_tag="O0305_003041"
                     /note="aph(3')-IIIa"
     CDS             1377..2171
                     /locus_tag="O0305_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCY0276093.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2447..3018
                     /locus_tag="O0305_003042"
                     /pseudo
     CDS             2447..3018
                     /locus_tag="O0305_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT         1078 a          508 c          736 g          904 t
ORIGIN      
        1 attagtgatt agctatattt tttactttgc aacagaacca tcatttttat taaaatactc
       61 ttttgtaaaa tctttctgct cttcaatgta tttaatttct ttacctctaa atctattata
      121 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
      181 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
      241 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
      301 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
      361 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
      421 gatatcatgg aaggtcggca tcgaaacagg ctttaaatga agtgtaggca agaactataa
      481 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
      541 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
      601 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
      661 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
      721 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
      781 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
      841 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
      901 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
      961 actgataaga taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg
     1021 cgaaaaaatt ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg
     1081 gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac
     1141 ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat
     1201 aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaaagct
     1261 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     1321 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     1381 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     1441 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     1501 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     1561 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     1621 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     1681 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     1741 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     1801 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     1861 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     1921 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     1981 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     2041 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     2101 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     2161 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     2221 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     2281 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     2341 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     2401 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     2461 cagagtaacc tctattctta ttcagctgca atcaaaaaaa taattcctgc caaagaaatt
     2521 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     2581 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     2641 cttctgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     2701 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     2761 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     2821 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     2881 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     2941 ccggaaagca gaatgataga gcctatatca ctggggtttt atgaacagaa ctggtactta
     3001 atcggttttg caggctaaga aatgaatata ggaattttcg tgtggatcgg attaaaagca
     3061 tttgcataga agaagagctc tgtcaaagcc atgatggctc tttggaacaa atattaaaac
     3121 aaatgctggt tctgttgcaa agtaaaaaaa tatagctaac cactaattta tcatgtcagt
     3181 gttcgcttaa cttgctagca tgatgctaat ttcgtggcat ggcgaa
//
LOCUS       DAKTIP010000486         1647 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.333, whole genome shotgun sequence.
ACCESSION   DAKTIP010000486 DAKTIP010000000
VERSION     DAKTIP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.333"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            <1..855
                     /locus_tag="O0305_003043"
     CDS             <1..855
                     /locus_tag="O0305_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCY0276094.1"
                     /translation="AIVKYNSFTKAAQFLHISQPSLTATIKKMEADLGYDLFTRSTKD
                     IKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTNQWIANLIRKH
                     HSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPLYEESYILLAP
                     KETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETDRFESAVGFVH
                     LGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQVHTFVQQCQDY
                     LYGLLEAL"
     gene            977..1396
                     /locus_tag="O0305_003044"
     CDS             977..1396
                     /locus_tag="O0305_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB1/FosB3 family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCY0276095.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLENRLNYYKEAKPHMTFYK"
BASE COUNT          638 a          262 c          232 g          515 t
ORIGIN      
        1 gctatcgtga aatataatag ttttactaaa gctgcacaat ttttacatat tagccagcca
       61 tctttaactg ctacgattaa aaaaatggaa gcagatttag gttatgactt atttacacgt
      121 tcaacaaaag acatcaagat taccgaaaaa ggaatacagt tttatcgtta tgcgagcgaa
      181 ttagttcaac aatatcgatc cacgatggaa aaaatgtatg atttaagcgt tacatcagaa
      241 ccaaggataa aaattgggac tcttgaatct acgaatcaat ggattgcgaa tttaattcga
      301 aagcaccatt ccgactaccc tgaacagcaa tatcgtttat atgaaataca tgataaacat
      361 caatctatag agcaattact gaattttaat attcatttag ctataacaaa tgaaaaaata
      421 acccacgaag atataagatc cattccttta tatgaggaat cttacatttt attagcaccc
      481 aaggaaacat ttaaaaatca aaattgggta gatgttgaaa atttgccact catattacca
      541 aacaaaaatt ctcaagtgcg caaacactta gatgactatt ttaatagaag aaatattcgt
      601 ccaaatgtcg ttgtagaaac agatcgattc gaatcagcag ttggatttgt tcatctcggc
      661 ttaggttacg ctatcattcc gagattttat taccaatcat ttcacacgtc taatttagaa
      721 tataaaaaaa ttcgtccaaa cttaggccga aaaatttata tcaattacca taaaaaacgc
      781 aaacactccg aacaagtaca tacattcgta caacaatgcc aagattattt atatggactt
      841 ttagaggctc tttaacttaa gttattagag cctcttatgc agttgcgcag atcatcgtat
      901 aaaaattaat gacgtcattt caaaaatcga tacaaaaata atttattata aaaattctaa
      961 gaaagaagtg aagcagatgt taaaatctat taatcatata tgcttttcag tcagaaattt
     1021 aaacgattca atacattttt atagagatat tttacttggg aaattgctat tgactggtaa
     1081 aaaaactgct tattttgagc ttgcaggcct atggattgct ttaaatgaag aaaaagatat
     1141 accacgtaat gaaattcact tttcatatac acatatagct ttcactatag atgacagcga
     1201 atttaaatat tggcatcaga ggttaaaaga taataacgtg aatattttag aaggaagagt
     1261 tagagatatt agagatagac aatcaattta ctttaccgac cctgatggtc ataagctaga
     1321 attacatact ggcacacttg agaacagatt aaattattat aaagaggcta aaccacatat
     1381 gacattttac aaataaggtg tcattataaa aaggcctctt gaactccgtt aaaattttaa
     1441 ttaattatta tataataaga gaacttttca aacaatacag ttgttatttt tgctatttca
     1501 acaaacataa ataagcagta agatgactac aacttaagag tcttcttact gcaattattt
     1561 ttcaaatata tcaacgttaa tataacttct attaagaaat actcacattc tgccctgcaa
     1621 tgcaaatctc gtcacatata aatattt
//
LOCUS       DAKTIP010000487         1513 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.342, whole genome shotgun sequence.
ACCESSION   DAKTIP010000487 DAKTIP010000000
VERSION     DAKTIP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.342"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            20..1372
                     /gene="tet(38)"
                     /locus_tag="O0305_003045"
     CDS             20..1372
                     /gene="tet(38)"
                     /locus_tag="O0305_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCY0276096.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT          470 a          222 c          257 g          564 t
ORIGIN      
        1 gtcattggtg tagatgatta tgaatgttga atattctaaa ataaagaaag cagtacctat
       61 tttattattc ttatttgtat tcagtttggt tatagacaac tcatttaaat tgatttctgt
      121 agccattgct gatgacttaa acatatctgt aacgacagta agttggcaag cgacattagc
      181 cggtttagta attggtattg gcgctgtagt atacgcttca ttatctgatg ccattagtat
      241 acgcacacta tttatttatg gcgtgatatt aatcattatc ggatcaatta ttggttacat
      301 tttccaacat caattcccat tacttttagt tggacgtatt attcaaactg ccggtttagc
      361 tgctgcagag acattatatg tgatatatgt tgcaaagtat ctttctaaag aggaccagaa
      421 gacttacctt ggcttaagta cgagcagtta ttccttgtca ttagttatcg gtacattatc
      481 aggtggattt atttctacgt atttacactg gacaaatatg tttttaattg cattaatcgt
      541 agtatttacg ttgccattcc tatttaaatt attaccaaaa gaaaataata cgaataaagc
      601 tcatttagat tttgttggct taattctagt ggcaactatt gctacaacag tcatgctgtt
      661 tattacgaac tttaattggt tatatatgat tggtgcctta attgcgatta tcgtttttgc
      721 gctatatatt aaaaatgcgc aacgtccatt agtaaataaa tcatttttcc aaaataaacg
      781 ttatgcttca tttttattta tagtatttgt aatgtatgct atccaattgg gttatatttt
      841 tacgttccca ttcataatgg agcaaattta tcatctgcaa ctagacacaa catcactgtt
      901 attagtaccg ggttatatag tagcagtcat tgttggtgca ctaagtggta aaatcggcga
      961 atatctgaat tcaaaacaag cgattatcac agcaattatt ttaatagcac tgagcttgat
     1021 tttacctgca tttgcagtag gtaatcacat ttcaatcttc gtcatttcta tgatattctt
     1081 tgcaggtagc tttgctttaa tgtatgcacc tttacttaac gaagccatta aaacaataga
     1141 tcttaatatg acaggtgtgg ctattggttt ttataattta attattaatg tggcggtatc
     1201 tgtaggtatt gcgattgctg cggctctaat cgattttaaa gcattaaatt tcccaggcaa
     1261 tgatgcatta agttcacatt tcggtattat tttaattatt ttaggtttaa tgagtattgt
     1321 cggattagtt ttattcgtca gcttaaatcg ttggacacaa tctgaaaaat aaatagatat
     1381 aaattcgcga gatatattcg tatttatagt aaaattaaat aaagagatta tataacacga
     1441 ggagtagtaa gtatgaaatt tgagaaatat atagatcaca ctttattgaa gcctgagtca
     1501 acacgtacgc aaa
//
LOCUS       DAKTIP010000488          722 bp    DNA     linear   BCT 08-DEC-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate 3257B
            SAMN04868227-rid16189993.guided.427, whole genome shotgun sequence.
ACCESSION   DAKTIP010000488 DAKTIP010000000
VERSION     DAKTIP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04868227
            Sequence Read Archive: SRR5415240
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-DEC-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001516813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001516813.1
            Genome Coverage       :: 86x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/08/2022 18:24:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,982
            Genes (coding)                    :: 2,911
            CDSs (with protein)               :: 2,911
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 32 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04868227-rid16189993.guided.427"
                     /isolate="3257B"
                     /isolation_source="nasal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /geo_loc_name="USA: TX"
                     /collection_date="2010"
                     /collected_by="EBrown"
     gene            34..513
                     /locus_tag="O0305_003046"
     CDS             34..513
                     /locus_tag="O0305_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCY0276097.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            528..>722
                     /locus_tag="O0305_003047"
     CDS             528..>722
                     /locus_tag="O0305_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCY0276098.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPA"
BASE COUNT          264 a          113 c          130 g          215 t
ORIGIN      
        1 aaaaaatgaa atgatagaag aaggaggata attatgactt tatccattct agttgcacat
       61 gacttgcaac gagtaattgg ttttgaaaat caattacctt ggcacctacc aaatgatttg
      121 aagcatgtta aaaaattatc aacaggtcat actttagtaa tgggtcgtaa gacatttgaa
      181 tcgattggta aaccactacc gaatcgtcga aatgttgtac ttacttcaga tacaagtttc
      241 aacgtagagg gcgttgatgt aattcactct attgaagata tttaccaact accgggccat
      301 gttttcatat ttggagggca aacattattt gaagaaatga ttgataaagt ggacgacatg
      361 tatattactg ttattgaagg taaattccgt ggtgatacgt tctttccacc ttatacattt
      421 gaagactggg aagttgcctc ttcagttgaa ggtaaactag atgagaaaaa tacaattcca
      481 catacctttc tacatttaat tcgtaaaaaa taagggggaa aacgaccatg acaaaacaga
      541 ttatagtaac agactcaaca tccgatttat ctaaagaata cttagaagca aacaacattc
      601 atgtaattcc tttaagttta actattgaag gagcttcata cgttgaccaa gtagatatta
      661 catcagaaga atttattaat catattgaaa atgatgaaga tgtaaagaca agtcagccag
      721 cc
//
